LOCUS       DAJTUP010000001        95171 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTUP010000001 DAJTUP010000000
VERSION     DAJTUP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95171
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.001"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(91..1341)
                     /gene="icd"
                     /locus_tag="OO468_000001"
     CDS             complement(91..1341)
                     /gene="icd"
                     /locus_tag="OO468_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR6257270.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1513..2166
                     /gene="rluE"
                     /locus_tag="OO468_000002"
     CDS             1513..2166
                     /gene="rluE"
                     /locus_tag="OO468_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR6257271.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2176..2637
                     /gene="nudJ"
                     /locus_tag="OO468_000003"
     CDS             2176..2637
                     /gene="nudJ"
                     /locus_tag="OO468_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR6257272.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2691..3797
                     /gene="mnmA"
                     /locus_tag="OO468_000004"
     CDS             2691..3797
                     /gene="mnmA"
                     /locus_tag="OO468_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR6257273.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3833..4474
                     /gene="hflD"
                     /locus_tag="OO468_000005"
     CDS             3833..4474
                     /gene="hflD"
                     /locus_tag="OO468_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR6257274.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4478..5848
                     /gene="purB"
                     /locus_tag="OO468_000006"
     CDS             4478..5848
                     /gene="purB"
                     /locus_tag="OO468_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR6257275.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6017..6688
                     /gene="phoP"
                     /locus_tag="OO468_000007"
     CDS             6017..6688
                     /gene="phoP"
                     /locus_tag="OO468_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR6257276.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6688..8148
                     /gene="phoQ"
                     /locus_tag="OO468_000008"
     CDS             6688..8148
                     /gene="phoQ"
                     /locus_tag="OO468_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR6257277.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8945..9226)
                     /locus_tag="OO468_000009"
     CDS             complement(8945..9226)
                     /locus_tag="OO468_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257278.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9240..10901)
                     /locus_tag="OO468_000010"
     CDS             complement(9240..10901)
                     /locus_tag="OO468_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR6257279.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10885..11211)
                     /locus_tag="OO468_000011"
     CDS             complement(10885..11211)
                     /locus_tag="OO468_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR6257280.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11267..11449)
                     /locus_tag="OO468_000012"
     CDS             complement(11267..11449)
                     /locus_tag="OO468_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257281.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11433..11873)
                     /locus_tag="OO468_000013"
     CDS             complement(11433..11873)
                     /locus_tag="OO468_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6257282.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11873..12169)
                     /locus_tag="OO468_000014"
     CDS             complement(11873..12169)
                     /locus_tag="OO468_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR6257283.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12166..12504)
                     /locus_tag="OO468_000015"
     CDS             complement(12166..12504)
                     /locus_tag="OO468_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR6257284.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12501..13712)
                     /locus_tag="OO468_000016"
     CDS             complement(12501..13712)
                     /locus_tag="OO468_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR6257285.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13714..14286)
                     /locus_tag="OO468_000017"
     CDS             complement(13714..14286)
                     /locus_tag="OO468_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR6257286.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14326..15483)
                     /locus_tag="OO468_000018"
                     /pseudo
     CDS             complement(14326..15483)
                     /locus_tag="OO468_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15784..16008)
                     /locus_tag="OO468_000019"
     CDS             complement(15784..16008)
                     /locus_tag="OO468_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257287.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16150..16311)
                     /locus_tag="OO468_000020"
     CDS             complement(16150..16311)
                     /locus_tag="OO468_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR6257288.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16321..16626)
                     /locus_tag="OO468_000021"
     CDS             complement(16321..16626)
                     /locus_tag="OO468_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257289.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16623..16901)
                     /locus_tag="OO468_000022"
     CDS             complement(16623..16901)
                     /locus_tag="OO468_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257290.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17190..18938)
                     /locus_tag="OO468_000023"
     CDS             complement(17190..18938)
                     /locus_tag="OO468_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6257291.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18935..19234)
                     /locus_tag="OO468_000024"
     CDS             complement(18935..19234)
                     /locus_tag="OO468_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6257292.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19240..19473)
                     /locus_tag="OO468_000025"
     CDS             complement(19240..19473)
                     /locus_tag="OO468_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257293.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19475..19695)
                     /locus_tag="OO468_000026"
                     /pseudo
     CDS             complement(19475..19695)
                     /locus_tag="OO468_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19688..19909)
                     /locus_tag="OO468_000027"
     CDS             complement(19688..19909)
                     /locus_tag="OO468_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257294.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19899..20099)
                     /locus_tag="OO468_000028"
     CDS             complement(19899..20099)
                     /locus_tag="OO468_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR6257295.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20229..20408
                     /locus_tag="OO468_000029"
     CDS             20229..20408
                     /locus_tag="OO468_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257296.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20533..20862
                     /locus_tag="OO468_000030"
     CDS             20533..20862
                     /locus_tag="OO468_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257297.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20912..21100)
                     /locus_tag="OO468_000031"
     CDS             complement(20912..21100)
                     /locus_tag="OO468_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6257298.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21475..22704)
                     /locus_tag="OO468_000032"
     CDS             complement(21475..22704)
                     /locus_tag="OO468_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6257299.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22953..24074
                     /gene="roxA"
                     /locus_tag="OO468_000033"
                     /gene_synonym="ycfD"
     CDS             22953..24074
                     /gene="roxA"
                     /locus_tag="OO468_000033"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR6257300.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24123..25349)
                     /gene="pepT"
                     /locus_tag="OO468_000034"
     CDS             complement(24123..25349)
                     /gene="pepT"
                     /locus_tag="OO468_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR6257301.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25599..26735
                     /gene="potA"
                     /locus_tag="OO468_000035"
     CDS             25599..26735
                     /gene="potA"
                     /locus_tag="OO468_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR6257302.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26719..27576
                     /gene="potB"
                     /locus_tag="OO468_000036"
     CDS             26719..27576
                     /gene="potB"
                     /locus_tag="OO468_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR6257303.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27573..28367
                     /gene="potC"
                     /locus_tag="OO468_000037"
     CDS             27573..28367
                     /gene="potC"
                     /locus_tag="OO468_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR6257304.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28364..29410
                     /gene="potD"
                     /locus_tag="OO468_000038"
     CDS             28364..29410
                     /gene="potD"
                     /locus_tag="OO468_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR6257305.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29566..30387)
                     /gene="cobB"
                     /locus_tag="OO468_000039"
     CDS             complement(29566..30387)
                     /gene="cobB"
                     /locus_tag="OO468_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR6257306.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30403..31314)
                     /gene="nagK"
                     /locus_tag="OO468_000040"
     CDS             complement(30403..31314)
                     /gene="nagK"
                     /locus_tag="OO468_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR6257307.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31343..32587)
                     /gene="lolE"
                     /locus_tag="OO468_000041"
     CDS             complement(31343..32587)
                     /gene="lolE"
                     /locus_tag="OO468_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR6257308.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32587..33288)
                     /gene="lolD"
                     /locus_tag="OO468_000042"
     CDS             complement(32587..33288)
                     /gene="lolD"
                     /locus_tag="OO468_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR6257309.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33281..34480)
                     /gene="lolC"
                     /locus_tag="OO468_000043"
     CDS             complement(33281..34480)
                     /gene="lolC"
                     /locus_tag="OO468_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR6257310.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34742..35815
                     /locus_tag="OO468_000044"
     CDS             34742..35815
                     /locus_tag="OO468_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6257311.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35943..39389
                     /gene="mfd"
                     /locus_tag="OO468_000045"
     CDS             35943..39389
                     /gene="mfd"
                     /locus_tag="OO468_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR6257312.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39532..40494
                     /gene="ldtC"
                     /locus_tag="OO468_000046"
     CDS             39532..40494
                     /gene="ldtC"
                     /locus_tag="OO468_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR6257313.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40577..40789)
                     /locus_tag="OO468_000047"
     CDS             complement(40577..40789)
                     /locus_tag="OO468_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6257314.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41074..41706
                     /gene="comR"
                     /locus_tag="OO468_000048"
     CDS             41074..41706
                     /gene="comR"
                     /locus_tag="OO468_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR6257315.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41768..42307)
                     /locus_tag="OO468_000049"
     CDS             complement(41768..42307)
                     /locus_tag="OO468_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6257316.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42534..43838)
                     /gene="ndh"
                     /locus_tag="OO468_000050"
     CDS             complement(42534..43838)
                     /gene="ndh"
                     /locus_tag="OO468_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR6257317.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44246..44788)
                     /locus_tag="OO468_000051"
     CDS             complement(44246..44788)
                     /locus_tag="OO468_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6257318.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44811..45836)
                     /gene="nagZ"
                     /locus_tag="OO468_000052"
     CDS             complement(44811..45836)
                     /gene="nagZ"
                     /locus_tag="OO468_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR6257319.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45847..46671)
                     /gene="thiK"
                     /locus_tag="OO468_000053"
     CDS             complement(45847..46671)
                     /gene="thiK"
                     /locus_tag="OO468_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR6257320.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46652..47293)
                     /gene="lpoB"
                     /locus_tag="OO468_000054"
     CDS             complement(46652..47293)
                     /gene="lpoB"
                     /locus_tag="OO468_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR6257321.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47308..47685)
                     /locus_tag="OO468_000055"
     CDS             complement(47308..47685)
                     /locus_tag="OO468_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR6257322.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47688..48047)
                     /gene="hinT"
                     /locus_tag="OO468_000056"
     CDS             complement(47688..48047)
                     /gene="hinT"
                     /locus_tag="OO468_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR6257323.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48380..50565
                     /gene="fhuE"
                     /locus_tag="OO468_000057"
                     /pseudo
     CDS             48380..50565
                     /gene="fhuE"
                     /locus_tag="OO468_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50625..52058)
                     /gene="ptsG"
                     /locus_tag="OO468_000058"
     CDS             complement(50625..52058)
                     /gene="ptsG"
                     /locus_tag="OO468_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR6257324.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52353..53150)
                     /locus_tag="OO468_000059"
     CDS             complement(52353..53150)
                     /locus_tag="OO468_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6257325.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53161..54165)
                     /gene="holB"
                     /locus_tag="OO468_000060"
     CDS             complement(53161..54165)
                     /gene="holB"
                     /locus_tag="OO468_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR6257326.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54162..54803)
                     /locus_tag="OO468_000061"
     CDS             complement(54162..54803)
                     /locus_tag="OO468_000061"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR6257327.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54793..55815)
                     /gene="yceG"
                     /locus_tag="OO468_000062"
     CDS             complement(54793..55815)
                     /gene="yceG"
                     /locus_tag="OO468_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR6257328.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55818..56627)
                     /gene="pabC"
                     /locus_tag="OO468_000063"
     CDS             complement(55818..56627)
                     /gene="pabC"
                     /locus_tag="OO468_000063"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR6257329.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56747..57988)
                     /gene="fabF"
                     /locus_tag="OO468_000064"
     CDS             complement(56747..57988)
                     /gene="fabF"
                     /locus_tag="OO468_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR6257330.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58075..58311)
                     /gene="acpP"
                     /locus_tag="OO468_000065"
     CDS             complement(58075..58311)
                     /gene="acpP"
                     /locus_tag="OO468_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6257331.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58522..59256)
                     /gene="fabG"
                     /locus_tag="OO468_000066"
     CDS             complement(58522..59256)
                     /gene="fabG"
                     /locus_tag="OO468_000066"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR6257332.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59269..60198)
                     /gene="fabD"
                     /locus_tag="OO468_000067"
     CDS             complement(59269..60198)
                     /gene="fabD"
                     /locus_tag="OO468_000067"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR6257333.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60214..61167)
                     /gene="fabH"
                     /locus_tag="OO468_000068"
     CDS             complement(60214..61167)
                     /gene="fabH"
                     /locus_tag="OO468_000068"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR6257334.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61235..62305)
                     /gene="plsX"
                     /locus_tag="OO468_000069"
     CDS             complement(61235..62305)
                     /gene="plsX"
                     /locus_tag="OO468_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR6257335.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62386..62559)
                     /gene="rpmF"
                     /locus_tag="OO468_000070"
     CDS             complement(62386..62559)
                     /gene="rpmF"
                     /locus_tag="OO468_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR6257336.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62611..63132)
                     /gene="yceD"
                     /locus_tag="OO468_000071"
     CDS             complement(62611..63132)
                     /gene="yceD"
                     /locus_tag="OO468_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR6257337.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63331..63915
                     /gene="yceF"
                     /locus_tag="OO468_000072"
     CDS             63331..63915
                     /gene="yceF"
                     /locus_tag="OO468_000072"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR6257338.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64027..64986)
                     /gene="rluC"
                     /locus_tag="OO468_000073"
     CDS             complement(64027..64986)
                     /gene="rluC"
                     /locus_tag="OO468_000073"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR6257339.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65559..68750
                     /gene="rne"
                     /locus_tag="OO468_000074"
     CDS             65559..68750
                     /gene="rne"
                     /locus_tag="OO468_000074"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR6257340.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68947..69889)
                     /gene="flgL"
                     /locus_tag="OO468_000075"
                     /pseudo
     CDS             complement(68947..69889)
                     /gene="flgL"
                     /locus_tag="OO468_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69901..71543)
                     /gene="flgK"
                     /locus_tag="OO468_000076"
                     /pseudo
     CDS             complement(69901..71543)
                     /gene="flgK"
                     /locus_tag="OO468_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71609..72550)
                     /gene="flgJ"
                     /locus_tag="OO468_000077"
     CDS             complement(71609..72550)
                     /gene="flgJ"
                     /locus_tag="OO468_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR6257341.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72550..73647)
                     /gene="flgI"
                     /locus_tag="OO468_000078"
     CDS             complement(72550..73647)
                     /gene="flgI"
                     /locus_tag="OO468_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR6257342.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73659..74357)
                     /gene="flgH"
                     /locus_tag="OO468_000079"
     CDS             complement(73659..74357)
                     /gene="flgH"
                     /locus_tag="OO468_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR6257343.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74410..75192)
                     /gene="flgG"
                     /locus_tag="OO468_000080"
     CDS             complement(74410..75192)
                     /gene="flgG"
                     /locus_tag="OO468_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR6257344.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75330..76081)
                     /gene="flgF"
                     /locus_tag="OO468_000081"
                     /pseudo
     CDS             complement(75330..76081)
                     /gene="flgF"
                     /locus_tag="OO468_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76101..77240)
                     /locus_tag="OO468_000082"
     CDS             complement(76101..77240)
                     /locus_tag="OO468_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR6257345.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(77265..77960)
                     /gene="flgD"
                     /locus_tag="OO468_000083"
     CDS             complement(77265..77960)
                     /gene="flgD"
                     /locus_tag="OO468_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR6257346.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77972..78375)
                     /gene="flgC"
                     /locus_tag="OO468_000084"
                     /pseudo
     CDS             complement(77972..78375)
                     /gene="flgC"
                     /locus_tag="OO468_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78379..78795)
                     /gene="flgB"
                     /locus_tag="OO468_000085"
     CDS             complement(78379..78795)
                     /gene="flgB"
                     /locus_tag="OO468_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR6257347.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78950..79609
                     /gene="flgA"
                     /locus_tag="OO468_000086"
     CDS             78950..79609
                     /gene="flgA"
                     /locus_tag="OO468_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR6257348.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79685..79978
                     /gene="flgM"
                     /locus_tag="OO468_000087"
     CDS             79685..79978
                     /gene="flgM"
                     /locus_tag="OO468_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR6257349.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79983..80399
                     /gene="flgN"
                     /locus_tag="OO468_000088"
     CDS             79983..80399
                     /gene="flgN"
                     /locus_tag="OO468_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR6257350.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80439..81974)
                     /gene="murJ"
                     /locus_tag="OO468_000089"
     CDS             complement(80439..81974)
                     /gene="murJ"
                     /locus_tag="OO468_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR6257351.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82174..83007)
                     /locus_tag="OO468_000090"
     CDS             complement(82174..83007)
                     /locus_tag="OO468_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6257352.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83009..83656)
                     /locus_tag="OO468_000091"
     CDS             complement(83009..83656)
                     /locus_tag="OO468_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR6257353.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83667..84251)
                     /gene="rimJ"
                     /locus_tag="OO468_000092"
     CDS             complement(83667..84251)
                     /gene="rimJ"
                     /locus_tag="OO468_000092"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6257354.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84487..85695
                     /gene="mdtH"
                     /locus_tag="OO468_000093"
     CDS             84487..85695
                     /gene="mdtH"
                     /locus_tag="OO468_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR6257355.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85759..86406
                     /gene="grxB"
                     /locus_tag="OO468_000094"
     CDS             85759..86406
                     /gene="grxB"
                     /locus_tag="OO468_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR6257356.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86540..87100
                     /locus_tag="OO468_000095"
     CDS             86540..87100
                     /locus_tag="OO468_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6257357.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87206..88252
                     /gene="pyrC"
                     /locus_tag="OO468_000096"
     CDS             87206..88252
                     /gene="pyrC"
                     /locus_tag="OO468_000096"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR6257358.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88326..88571
                     /gene="dinI"
                     /locus_tag="OO468_000097"
     CDS             88326..88571
                     /gene="dinI"
                     /locus_tag="OO468_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR6257359.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88861..89115
                     /gene="bssS"
                     /locus_tag="OO468_000098"
     CDS             88861..89115
                     /gene="bssS"
                     /locus_tag="OO468_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR6257360.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89230..90348
                     /gene="solA"
                     /locus_tag="OO468_000099"
     CDS             89230..90348
                     /gene="solA"
                     /locus_tag="OO468_000099"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR6257361.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90396..90509
                     /locus_tag="OO468_000100"
     CDS             90396..90509
                     /locus_tag="OO468_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR6257362.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90770..91336
                     /locus_tag="OO468_000101"
     CDS             90770..91336
                     /locus_tag="OO468_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR6257363.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91340..91915
                     /locus_tag="OO468_000102"
     CDS             91340..91915
                     /locus_tag="OO468_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR6257364.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91957..93009)
                     /locus_tag="OO468_000103"
     CDS             complement(91957..93009)
                     /locus_tag="OO468_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR6257365.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93234..94154
                     /gene="lpxL"
                     /locus_tag="OO468_000104"
     CDS             93234..94154
                     /gene="lpxL"
                     /locus_tag="OO468_000104"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR6257366.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94326..94523
                     /locus_tag="OO468_000105"
     CDS             94326..94523
                     /locus_tag="OO468_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR6257367.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94547..>95171
                     /locus_tag="OO468_000106"
     CDS             94547..>95171
                     /locus_tag="OO468_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6257368.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLS"
BASE COUNT        22886 a        25238 c        23484 g        23563 t
ORIGIN      
        1 aaatatgctc gatatgctat taataatatc cacataaata aaacaacggg cgtgttatac
       61 gcccgtttaa atatttaaca catgtagtga ttacatattc ttgatgatcg catcaccaaa
      121 ctctgaacat ttcagcagtt tagcgccttc catcagacgc tcgaagtcat aggttacggt
      181 cttggcgttg atcgcgcctt ccataccttt aacaatcagg tctgcggctt cagtccagcc
      241 catatggcgt aacatcatct cagcggagag aataatagag ccagggttta ctttgtcctg
      301 accggcgtat ttcggcgcag taccgtgggt ggcttcaaac agggcgcatt cgtcaccgat
      361 gtttgctcca ggggcgatac cgataccgcc aacctgcgct gccagagcgt cagaaatgta
      421 gtcaccgttc aggttcatac aggcgataac atcatattca gccggacgca gcaggatctg
      481 ttgcaggaat gcatcagcaa tcacgtcttt aatgacgatc tctttgccgg tattcgggtt
      541 tttaactttc agccacgggc cgccgtcgat cagttcaccg ccaaattctt cacgcgccag
      601 ctggtagccc cagtctttaa acgcgccttc ggtgaacttc atgatgttgc ctttgtgcac
      661 cagggtcaca gagtcacgat cgttagcaat tgcgtattcg atcgctgcac gaaccagacg
      721 tttggtgcct tcttcagaac acggcttaat accgataccg caatgttccg ggaagcgaat
      781 tttcttcacg cccatctctt cacgcaggaa tttaatcact ttctcggcgt cggcagagtc
      841 agctttccat tcgatacccg cataaatgtc ttccgagttt tcacggaaga taaccatatc
      901 ggtcagttca gggtgtttaa ccgggcttgg agtgccctga tagtaacgta ccggacgcag
      961 gcagatgtag agatccagtt cctgacgcag ggcaacgttg agagaacgaa taccgccacc
     1021 aaccggagtg gtcagcgggc ctttaatggc aacgcgatat tcacgaatca gatcaagggt
     1081 ttcagcaggc agccagacat cctgaccata aacctgtgtg gatttttcac cggtgtaaat
     1141 ttccatccag gagattttac gctcgccttt ataggctttc tcgactgcag cgtcgaccac
     1201 tttcagcatg gctggggtta catctacacc gattccatca ccttcaatgt aagggataat
     1261 cggattttca ggaacgttga gtttgccgtt ttgcagggtg atcttcttgc cttgtgccgg
     1321 aacaactact ttactttcca ttcacctctc cttcgagcgc tactggtttg ctcgtctgcc
     1381 accacgttgc atatgcgttt gcgtcctgcg atacggatgc tttagagcaa ttttttgtta
     1441 atgatttgta attgacttgt caatactacc ctattgtttg ccgtcatgaa agatgcgcgt
     1501 tattaggcta taatgcggca attcataatc tctgaaaata ccatgcaaaa aacttctttt
     1561 agaaatcacc aggttaagcg attcagctcg caacgttcta ccaggcgtaa acatgaaaac
     1621 cagcccacgc gtgtgatcct cttcaataaa ccctacgatg ttcttccgca gttcaccgat
     1681 gaaaccggac gcaaaacatt aaaagaattc atcccggttc agggcgttta tgcagcaggt
     1741 cgccttgacc gcgatagcga agggttgctg gtgctgacca ataacggcgc gttgcaggcg
     1801 cgtttaaccc agccgggtaa acgcaccgga aaaatctatt atgtgcaggt ggaaggtatt
     1861 cccacacaag acgcacttga agccttgcgc aatggcgtaa ccttaaatga tggccctacc
     1921 ctgcccgcag gcgcggagct ggttgacgaa cccgcgtggt tatggccgcg gaatccacca
     1981 attcgtgaac gcaaaagtat tcccaccagc tggctgaaga tcaccttata tgaaggacgt
     2041 aatcgccagg tgcgccgcat gaccgcccat gttggtttcc ccacgctgcg actgattcgc
     2101 tatgcgatgg gagattactc tttggataat cttgccaatg gcgaatggcg agaagtgaca
     2161 gattaaggat gcaaaatgtt taaaccgcac gttaccgttg cttgcgtggt gcacgcagaa
     2221 ggcaaatttt tagtcgttga agagacgatt aatggtaaag cgttatggaa ccaacctgcc
     2281 gggcatctgg aagccgatga aaccttagtg gaagccgccg cccgtgaact gtgggaagaa
     2341 accggcatca gcgcgcagcc gcaacacttt attcgtatgc atcagtggat tgcgccagat
     2401 aaaacgccgt ttttgcgttt cctctttgcc attgagcttg agcaaatatg cccgacccag
     2461 cctcatgaca gcgatatcga ctgctgccgt tgggtcagcg ccgaagaaat tttacaggcg
     2521 tcaaatcttc gttcgccgct ggtggcggaa agtattcgtt gttatcaaag cgggcaacgt
     2581 tatccgctgg agatgattgg cgattttaac tggcctttta caaagggtgt catctaaaag
     2641 gctgcgtgat agaatacgcc gccttgaagt tcaatgtcgt gagtgatcca atgtctgaaa
     2701 ccgcaaaaaa agtaatcgtc ggcatgtccg gcggtgtcga ttcctccgtt tctgcctggc
     2761 tgttgcaaca acagggatat caggtcgaag gcctgtttat gaagaactgg gaagaagacg
     2821 acggtgagga atattgcaca gcagcagcgg atctggctga tgcccaggcg gtctgcgaca
     2881 agctcggcat tgaactgcac accgttaact ttgctgccga gtactgggac aacgtcttcg
     2941 aactgttcct tgccgaatat aaagccggtc gcacgccgaa tccggatatt ctgtgcaaca
     3001 aggagatcaa atttaaagcc ttcctcgaat ttgccgccga agatttaggt gccgattata
     3061 tcgctaccgg tcattacgta cgtcgggccg atgtcgatgg caagagccgc ctgctgcgtg
     3121 gtctggacag caataaagac cagagctact tcctttatac gctcagccat gagcagattg
     3181 cgcaaagcct gttcccggta ggcgaactgg aaaaaccgca ggtgcgtaag attgctgaag
     3241 atcttggtct ggtcaccgcg aagaaaaaag actctaccgg catttgcttc attggcgaac
     3301 gtaaattccg cgagttcctg ggccgttatc tcccggcgca accgggcaaa atcattaccg
     3361 tcgatggcga tgaaattggt gagcaccagg ggctgatgta tcacactctc ggtcagcgta
     3421 aaggtctggg tatcggtggc accaaagaag gtaccgaaga accgtggtat gtggtggaca
     3481 aagacgtcga aaacaacatt ctggttgtcg ctcagggcca tgaacacccg cggctgatgt
     3541 ctgtcgggtt gattgcccag cagctgcact gggtcgatcg cgaaccattc accggcacta
     3601 tgcgttgcac ggtaaaaacc cgctatcgcc agaccgacat tccgtgcacc gtcaaggcgc
     3661 tggacgacga tcgcattaaa gtgattttcg atgagccggt tgccgccgtg acgccgggcc
     3721 agtctgccgt cttctataac ggtgaagtgt gcctcggtgg cggtattatt gagcagcgtc
     3781 tgccgctgcc ggtctgatta ttatctttac ttaaaacaag gaagcagtga acgtggcaaa
     3841 gaattactat gacatcaccc tcgccctggc cggtatttgt cagtcggcac gcctggtgca
     3901 acaactcgct caccaggggc attgtgatgc cgatgcgcta cacgtctcac tcaacagtat
     3961 tattgatatg aaccccagct cgacgctggc ggtttttggc ggtagcgaag ccaacctgcg
     4021 cgtcgggctg gaaaccctgc tcggcgtgct caatgccagc agttgccagg gcttaaacgc
     4081 cgaattaacc cgctacacac tcagcttgat ggtgcttgag cgcaaactct cctcagcgaa
     4141 aggcgcgctc gacactctgg gcaaccggat caacggcctg caacgccagc tcgaacactt
     4201 cgatttacag tccgaaacgc tgatgagcgc gatggctgct atctatgttg atgtgatcag
     4261 cccgcttggc ccgcgcattc aggtcaccgg ttcccctgct gtactgcaaa gcccacaagt
     4321 gcaggcgaaa gttcgcgcaa ccctgctggc aggcattcgc gccgccgtgc tctggcacca
     4381 ggtcggcggc ggacgtctac aactgatgtt ttctcgtaat cgcctgacca ctcaggcaaa
     4441 acaaattctt gctcatttaa ccccggagtt gtgatctatg gaattatcct cactgaccgc
     4501 cgtttcccct gtcgatggac gctacggcga taaagtcagc gcgctgcgcg ggattttcag
     4561 cgaatatggt ttgctgaaat tccgtgtaca agttgaagta cgttggctgc aaaaactggc
     4621 cgcgcacgca gcgatcaagg aagttcctgc ttttgctgcc gacgcaatcg gttaccttga
     4681 tgcaattgtc gccaatttca gcgaagaaga tgccgcacgc atcaaaacca tcgagcgtac
     4741 cactaaccac gacgttaaag cggttgagta tttcctgaaa gaaaaagtgg cggagatccc
     4801 ggaactgcac gcggtttctg aattcatcca ctttgcctgt acttcggaag atatcaataa
     4861 cctctcccac gcattaatgc tgaaaaccgc gcgtgatgaa gtgatcctgc cgtactggcg
     4921 tcaactgatt gatggcatta aagatctcgc cgttcagtac cgcgatatcc cgctgctgtc
     4981 tcgtacccac ggtcagccag ccacgccgtc aaccatcggt aaagagatgg caaacgtcgc
     5041 ctaccgtatg gagcgccagc accgccagct taaccaggtg gagatcctcg gcaaaatcaa
     5101 cggcgcggtc ggtaactata acgcccacat cgccgcttac ccggaagttg actggcatca
     5161 gttcagcgaa gagttcgtca cctcgctggg tattcaatgg aacccgtaca ctacccagat
     5221 tgaaccgcac gactacattg ccgaactgtt tgattgcgtt gcgcgcttca acaccattct
     5281 gatcgacttt gaccgtgacg tctggggtta tatcgccctt aaccacttca aacagaaaac
     5341 cattgctggt gagattggtt cttccaccat gccgcataaa gttaacccga tcgacttcga
     5401 aaactccgaa gggaatctgg gcctttccaa cgcggtattg cagcatctgg caagcaaact
     5461 gccggtttcc cgctggcagc gtgacctgac cgactccacc gtgctgcgta acctcggcgt
     5521 gggtatcggt tatgcgctga ttgcgtatca atccaccctg aaaggcgtga gcaaactgga
     5581 agtgaaccgt gaccatctgc tggatgagct ggatcacaac tgggaagtgc tggcagaacc
     5641 aatccagaca gttatgcgtc gctatggcat cgaaaaaccg tacgagaagc tgaaagagct
     5701 tactcgcggt aagcgcgttg acgccgaagg catgaagcag tttatcgatg gtctggcgtt
     5761 gccagaagaa gagaaagccc gcctgaaagc gatgacgccg gctaactata ttggtcgagc
     5821 tatcacgatg gttgatgagc tgaaataagc ctcaaatcag tgccggatgg cgatgctgtc
     5881 cggcctgctt attaagatta tccacttttt cttttttcac tttacctccc ctccccgctg
     5941 gtttatttaa tgtttacccc cataaacaca taatcgcgtt acactatttt aataattaag
     6001 acagggagaa ataaaaatgc gcgtactggt tgttgaagac aatgcgttgt tacgtcacca
     6061 ccttaaagtt cagattcagg atgctggtca tcaggtcgat gacgcagaag atgccaaaga
     6121 agccgattat tatctcaatg aacatttacc ggatattgcg attgtcgatc tcggattgcc
     6181 tgacgaggac ggtctgtcac tgattcgccg ctggcgtagc aacgatgttt cactgccgat
     6241 tctggtatta accgcccgtg aaagctggca ggacaaagtc gaagtattaa gtgccggtgc
     6301 tgatgattac gtgactaaac cgtttcatat tgaagaggtg atggcgcgaa tgcaggcatt
     6361 aatgcggcgt aatagcggtc tggcttcaca ggtcatttcg ctccccccgt ttcaggttga
     6421 tctctctcgc cgtgaattat ctattaatga cgaagtgatc aaactgaccg cgttcgaata
     6481 caccattatg gaaacgttga tacgcaataa tggcaaagtg gtcagcaaag attcgttaat
     6541 gctacaactc tatccggatg cggagctgcg ggaaagccat accattgatg tactgatggg
     6601 acgtctgcgc aaaaaaattc aggcacaata tccgcaagaa gtcattacca ccgttcgcgg
     6661 ccagggctac ctgttcgaat tgcgctgatg aaaaaattac tgcgtctttt tttcccgctc
     6721 tcgctgcggg tacgttttct gttggcaacg gcagcggtag tactggtgct ttcgcttgcc
     6781 tacggaatgg tcgcgctgat cggttatagc gtcagtttcg ataaaactac gtttcggctg
     6841 ttacgtggcg agagcaatct gttctatacc cttgcgcagt gggaaaacaa taagttgcat
     6901 gtcgagttac ccgaaaatat cgacaagcaa agccccacca tgacgctaat ttatgatgag
     6961 aacgggcagc ttttatgggc gcaacgtgac gtgccctggc tgatgaaaat gatccagcct
     7021 gactggctga aatcgaatgg ttttcatgaa attgaagcgg atgttaacga taccagcctc
     7081 ttgctgagtg gagatcattc gatacagcaa cagttgcagg aagtgcggga agatgatgac
     7141 gacgcggaga tgacccactc ggtggcggta aacgtctacc cggcaacatc gcggatgcca
     7201 aaattaacca ttgtggtggt ggataccatt ccggtggagc taaaaagttc ctatatggtc
     7261 tggagctggt ttatctatgt gctctcagcc aatctgctgt tagtgatccc gctgctgtgg
     7321 gtcgccgcct ggtggagttt acgccccatc gaagccctgg caaaagaagt ccgcgaactg
     7381 gaagaacata accgcgaatt gctcaatcca gccacaacgc gagaactgac cagtctggta
     7441 cgaaacctga accgattgtt aaaaagtgaa cgcgaacgtt acgacaaata ccgtacgacg
     7501 ctcaccgacc tgacccatag tctgaaaacg ccactggcgg tgctgcaaag tacgctgcgt
     7561 tctctgcgta gtgaaaagat gagcgtcagt gatgctgagc cggtaatgct ggagcaaatc
     7621 agccgcattt cacagcaaat tggctactac ctgcatcgtg ccagtatgcg cggcgggaca
     7681 ttgctcagcc gcgagctgca tccggtcgcc ccactgctgg acaatctcac ctcagcgctg
     7741 aacaaagtgt atcaacgcaa aggggtcaat atctctctcg atatttcgcc agagatcagc
     7801 tttgtcggtg agcagaacga ttttgtcgag gtgatgggca acgtgctgga taatgcctgt
     7861 aaatattgcc tcgagtttgt cgaaatttct gcaaggcaaa ccgacgagca tctctatatt
     7921 gtggtcgagg atgatggccc cggtattcca ttaagcaagc gagaggtcat tttcgaccgt
     7981 ggtcaacggg ttgatacttt acgccctggg caaggtgtag ggctggcggt agcccgcgaa
     8041 atcaccgagc aatatgaggg taaaatcgtc gccggagaga gcatgctggg cggtgcgcgg
     8101 atggaggtga tttttggtcg ccagcattct gcgccgaaag atgaataaat atgtcctttt
     8161 accactacat caagtcattg attcgatcaa acaattttca ttgaaaaagt gccaaaaatc
     8221 gcaaattgac tacaccatta actacaccgt tcggtgcact gtatgaaaca acgtggaaca
     8281 aatagacaca agaaatatac aggcgggtaa tctttccagg gggaagcgca ccaattcatg
     8341 aggggcgtta atgtcgatat ggggatcccc ataacggggc taccggattt tttttccggt
     8401 taacctttaa tcaggctggt gggttttaca tacccttgat caccgtaatg atgatccaga
     8461 tgtgcacctt ttccaccaat ggcggaaaag ctccaaccgt aacgatgatc gctatggttg
     8521 cctcgatggt ctgcgtggtc attatgaccg ttcagccagt gaccttaatc agaatttttc
     8581 gctaaaaaat gacgttggcc acgtcatccg gaaagcacaa aatcacgtcg atttttaatg
     8641 aacggttaaa ccgtcttttt gtcacggtgt gtcactggtt gtcactctcc gtcacgattg
     8701 aggtggtggt cattctgccc gaccgcgcat ctttgcgcta tcgggaatat caaacagtta
     8761 cctcgccgac ttcctcaaat tgaggtttcc gaaaatatca gagcgggtta gcgattaagc
     8821 tgacgagtaa ttaaactgcg aaaatttcgt agtttctgaa tatattccgg cctgctggtt
     8881 aacagtcctc agatgtgagg gatgtagcca gcgcaatttt gcgctttgct ttaatctcag
     8941 tgagttaccg ccgtaaccgc tccggcttct tccagtggta cgtgattttc tccttctccc
     9001 gatacagttc aacgcggcga ttgtaggcca gcatttccag aacgcggatc cgtatgtcgc
     9061 gcatatccgc atcattaagc tggataccat cacggcgcat cacctcagca acaacacgcg
     9121 cataattttc ggctgtcacg ctgtccggct gcgtggtctg ttcgtcagcc tgctggctga
     9181 ttccagagac gcggcggatt aatcgcagta tttcggcttc tgtcatgctg ctgacctcat
     9241 tacaccccgc taaattcttc cagtttctgg cggtggctgt cgcttatatc aaaagcaaaa
     9301 tcctcatgct ctgcctggaa tgtaccaaac gccatcagcg ccgctacgct cgggtctatc
     9361 ttgttcggtg attttttctt gttcggcttg atattggcgt tcgcgtcact ctgcatcacg
     9421 acgttactca tcgaccagga caacaccgga tcgccacgat gcacaatcac cctgcggtta
     9481 acaaaaactt cgaacgattt cgccgccgga ctgaatctga ggtaggtttg cgggaacggc
     9541 tccacctcaa aaccagcccc ctgtaattgc gtcctgagat gcgtggcgtt ccacgtatca
     9601 aagcccacca gcctgatatt aaatttctcc gcatcctgca tgatgtcatc tctgatccgg
     9661 tcgtaatcaa tgcagtcacc tggcgttgtg cgtatccagc ccgctttagc ccactggcga
     9721 tagacagcgc ggtttttatt ggcggggttc tgtagctgga actccggcag ataatgacgg
     9781 gaaatcagca taatggtttt accgaccgga aaggcatagc atacgctgga aatatcgctg
     9841 gttgatgata agtccagccc cgcgtaacac tcctgaccgt gtaaatcttc ctccgtgaac
     9901 gttccggcac actcagccca tgcaccatta cccatccacg gggtagtccc ctgacaccag
     9961 atattaaatc gctttgtcat catctccacc cactgcgacg gaataccccg cgctttctgg
    10021 atggttgagg ccagtttttc acgatccacg gaaacatcaa tattagggtt cgcctttatc
    10081 cacatcgccg gatcatcaac ctcgttttcg tcgtccagtt cgtaaatcag tacaaaaatt
    10141 gaatcgttaa cctcttcgcc gtccaggatc tggcagcaat aatcatagtg ctgtttacag
    10201 gctgaaacga cgttactgcc cgatgtggta atggcaaata acagcccttc gggacgtgcg
    10261 cccatcccca gctcaagcgc ggaataaacg ccgttatcgg ggtgtaggtg gtattcgtcc
    10321 acgatggaaa gactggggtt tgtcccctcg atggttgccg cttttgctgc cagcggcttt
    10381 aacaggctgt tgcttttcgg gtgtatcacc ttatgcgcct gaatattcac ccgcctgcgt
    10441 aacggtcggg ataaaaggca catctgacgc gcattatcaa acacgatccg cgcctggtca
    10501 cggctcaccg ctgcggtgta gatatcctgc tgaccgttct ccataatcag aaaccagtta
    10561 gccagaatcg cggcggtcgt ggatttcgca tttttgcgcg gcacttcgat aaaggcgctc
    10621 gtgtatttgc gccgtccggt ggccttaacc ttaaagccga ggatgcacgc aaaggcgaac
    10681 tgctgccacg gctccagctc aatgggtctg ccacgcatcg gtccttttac gtgcgggcac
    10741 accctggaaa aggcaataaa ccgctccaca acctcacgat cgaacgtgta aagggggctt
    10801 ttaaggtccg aaaagtaccg tttaacagcc tgttttaacc gtttacaggc cggaattttg
    10861 cccgttttta cgtcttctgc gtacttattc caggcggtca agctcgtcct cttcttctgt
    10921 ttccggtgga tttttacggc ggcttatcgg gtcaaaaccg agcaaggagg ccatttttat
    10981 catcactctt tcagcgtcgg attttgcgct taatgcgggg tttctgctct cgccgccctg
    11041 actgttaaca atgctgaacc cgcgcgccgc aaggtctgca acggctttcc ggtaaatgga
    11101 gtagttgacg caatacagtt ccagattgct ccagtcggcg ggggtcaggt ctccccgttc
    11161 cgcaagctgc cgcgattttt cccgccactg cttcacggcg atatcatcca ggtaggcggg
    11221 gtcgggtttg tcctgaaaac gccccccacc ccgtcataca gcctcatcag cgattgcgga
    11281 aacattccat aacctcgcgg tcacggtcgg ttaatcgctt cgctgtgatg cgttctgcgc
    11341 gtcctgacgc tttatcttta tgccctgttt cctgtgtctt ccatgcgtca cgctgcctta
    11401 taagtccacg gataaggcgg ttttgttccc gctcattcat cagcgtcata catccagtta
    11461 ttgcggttag cagcccgttc ttcctcttca cgaaatccac ctgcggcacg cttgcttttt
    11521 gttgccggat caagccattt cgttttctgg ttatgacacg cctggcacaa tggttgatgg
    11581 ttccattcgg gccagaagag aacatcatca ccgccattaa tcgggataat gtgatccacc
    11641 accacggcgg gcgtatatat ccccttctca aggcatcgca cgcataacgg gtttttactc
    11701 agatacatgg cgcggtattt gtcccactgt ctggaatatc cacgcgcgcg gcggtgtcct
    11761 cgtctggcat cctctgcacg ccatgccgcc cgcctgtgct cctcacactt gccggattta
    11821 acgcgcctgt tacagcctgg ttctgtgcat cgtcttaatg gttgccacgg catcagtacc
    11881 cccccacatc acggtaagcc gtccagagtg cgcctatcgt catgggtacg cgtgtttttg
    11941 cgttatccgc gacaatctgg cgattttcat acaggtgagc gataaacatc atgcagccaa
    12001 tctttatggc tggcgtgaac gccagcccgt catcaaagcg cctgcctatg tgtatctggc
    12061 acgcctcaag cgatgcggca atgtatccgc tgattaactg gtcttcatcg tcgccatcga
    12121 tgcggcaatg gagtttcact tcttccaggg tgataagttc ttctgtcatt tttccgcgcc
    12181 ctcacgacaa agaatttcaa ggcgtgtcct ggcggcatcc ggcagcggct gcccgatgat
    12241 attcagtaca cgccccgcca gcggccccgt actgaccttt atccggctgg tggcgttgat
    12301 gtccttccgg taccgtatcc agatccttac ggtaccaatc gccagctctg cgcccgatga
    12361 aatggcctcc ttgctgctga ccatgttcac gcttgcccag agtgtgtgac cgtcctccca
    12421 cgtttcgagt atttcgccgg ttatatccct ggtctgtttc agggtctgaa tcgttatcct
    12481 gtcacgcagt cgccctatgt tcattccggt ttttctccct cgctgatttt tacttcctgt
    12541 ttccatgcct ggctgaactc atcaccacca tcacgcggtg acagcccttc acgttcgcgg
    12601 gcttcgttcg gacacataac gtcggatttt atgccgcgct cataggtggc aaagcgttcg
    12661 ccaggtgtgg cccgtaacag gtccgcgctg tcaaactcca cctgataccg gataccaggt
    12721 acaggcgagg ccaccagcaa ggcgtttttt atctgctgct caaagttcgc cagccacggg
    12781 cgcatcgtca tggtgagaaa tgcgcggctt gcctcgctga agttgctgta ggtgctgttg
    12841 ctgtattcct gcaaaaaaat cggcgagacg ttgaacattc gggcgatgtc ttcaatggtg
    12901 aagcgacggg aggccagcca ttcggcatcc tgattactca tgcccagctg ctgatagctc
    12961 atcccccctt caaggatggg cgtttttccg gcgtttctgg cccctttgta gcgttccagt
    13021 gcggctaatg cctgtttgcc cttcacgccg tccagccatt cgcctgacgt gataacccct
    13081 gccgccatca tgccatcacg catcacgctc gcgccgtggc gttgttgggc cagcccaagc
    13141 cccagcgatt cgcggcagat ggttacaggt gagcgcccca taaaaccgtc atccgtggag
    13201 taacgaaggt ggagaacctc ccacggtaaa tagttgcgga tgtttccggt gtaggcgtca
    13261 gtgatgcagt agcgccagtt atgatcacct gtctgctcca cgttcaccga ctgcggcggg
    13321 taaggatgta aagccgccgg aaaaccatca cgcccccact gaataaccgc ataagcatta
    13381 ccacttaaca ggcagtagcg gatcatcatt cgcttaaact ggtagggtgt ctgccacgcg
    13441 ttcgggcgct cgttgaggat gtgatcgacc ggatgagaat caagccattc gcgggcctcc
    13501 ttccccttct cattgcgtac caggtacagg taacacggca tggtagccac cgcgtcagag
    13561 atgaccgtga cggcgttcat gacggcgggg agtgattccg ctgtcccaga tgatacgtac
    13621 tcacccgccc ccgtgttcga tgtgccggcc agcgccataa attcatcaag cgtcatgctg
    13681 cgctgctctt tttttcgtct gaaaggccac atatcacacc cccgctaaat ccgcccacca
    13741 gcggcgatta tccgcacgcg gcattttttc ggggtgctgc tcatacaggg aacggcgggc
    13801 cagctccacg ccggaatcgg ggtaagccgg taccgatgta acggtaattt cgtacagttc
    13861 cgccaccagc acggtgcgca cgcatggatc tgttgtggta tcccatacat ccttacggga
    13921 gcgaaagcca aagctcatcc ccgagatgtc cccacgctta accagttcga taacgtcgcg
    13981 ccctgtactg gtatccggtg gggtcagttc aaagcgtaac cctgtttcgt cctcttccag
    14041 tttcagcgtg ccggaacggg tgcgccccag taacatgctg tggtcatgct catacaggcc
    14101 gcgaacgtca ttacccgccg caagccattc agtaaacgcc ccccgctgga atttttcgta
    14161 aaactcaccc cataacagtt ctgaaagctt atcccagcga acaacgtagc ccgtcagcgt
    14221 accggctccg ctggtggtga tttccgatga ccggatttcc atactcttca taattttttc
    14281 acccataaaa cactaaaggg gctttttagc cccttctgta tgctgttaat cgtcgtcttc
    14341 cggcagtgcc agaatcttga tcgcgttcga atccaccacg ccaccgccta aatatttttg
    14401 cgtgaaaatt ttgatgaatc ctggttctgt caggttgtcc ggtctggtgc gaacacctgt
    14461 ttcgtgatca acaatgtagt aaccgcgttt gaagtcaccc agggcaataa cgttatcagg
    14521 cataaactcc agatattcga ccggaaggcc cagcaacgta tcaggatcac ccgcctgtaa
    14581 acggtcgcgc cagatataat caccgttcgc gttcttcacc ttctgaagtt ttgccgccgt
    14641 cgtggagtta accactcaga ccgcgttttt gcggtatttt ttacgtaatg caaatttcag
    14701 gtcgatcagc gggtccgcag atgtccacgc cagagattcg gaaggtttaa ttacctgcaa
    14761 cgtaccaaaa tcacgctctt tgtcgttctt ctctgcacgg ggtacggata aaaaaccttt
    14821 tgcttttttg tcaccgtcgc ccacaaccag atcgctttct tcggtttccg tgaaggtgtc
    14881 gccaatctca cccgtcagcc atgaaaggat gtccacatcg gaaaaatcca cgatttcctg
    14941 tgtggtgcgc gggtacgcat agaccggata cagcttaatg ctcacctcgt taatctgcgg
    15001 ggtgctggtc tgttcgcgtg ccttaccctc ttcaccgtgg ttaacggtcg caccgccagc
    15061 ggaaacaagc tgcttaaact cgttgctgct gattttcttc acggtacaga tgcgacgcat
    15121 ggtggattca tccgccagca ttcgcatgat ttcggtgttc agttcaggga taacggtata
    15181 accaccatca gcgggaacgc ctgtacttaa tgcgcgggtt tcaccggtca gaatgtagtt
    15241 acgtagttct gcggggtcag tggtctggct gcttttacct ggcttgctgc gctcttcgtc
    15301 tgcaatggct tcaaggcggg aaatgtcttt atcgagggat tcagctttag cgcgtaattc
    15361 gtcaaatttt gcgccctcag catcgttaag actgcggttt tctttttcag cgttctccag
    15421 catgtcgcgc atctgatttt taatggcggt tttctgctgg cgtaattcga ttattctctg
    15481 cataaaaaaa gtcctggggt taagtaagga actccaggac gcggcaaaca ctcaaccgtt
    15541 tttcataagg aaatcagtaa tcgcaccgat cgttttcccg cctggtaatg aatattggcg
    15601 agcacattaa caggcgggaa agtggcccca gcgtcctggc accacgggcg agaataatca
    15661 tgattcagtt cgggtaaaat atgccgatcc gctcagtgaa caacgtggaa caaccacgaa
    15721 caaataattt acaaaaaata acaaaaagcc ggattgctcc ggctgtatgt gctgtatggg
    15781 tgctcagtct ctgatccctt tcagaaattc tatcaatgca tctgtctgtt caggatttac
    15841 tgccagcatt tcaccggata gcgcgcaacg aacaaaacca tgatgatcct tttcaatcag
    15901 cgcccccgtt tccagaaatg cgcggtaatc agtgatattc atcgtctcca tgttgtcagc
    15961 atggtattta tcacgctgtt taagtatctc atcaaatttc atcggcattg cgttttcctc
    16021 tgttgtgtct gtttgtttca gatagtaact atgccggacc gtgacgaaaa gccagtagtg
    16081 ctccataccg tttcaaatga cgcaaaaaaa gccggattgc tccggctgcg tggtgtggtt
    16141 ctgatattcc tactctgcta atttgctcag ccccatgtct gcgtaagtgc gatttactgc
    16201 atttctcagg tctgcgtagt tctccgttgg cggctccggt gtgcccttgc tgctctgttt
    16261 taccagcggg tcgcccacca gctgcccgta agctgcaatt tgtgctgtca tattgccacc
    16321 tcagtgaagc gatccggcat tgttcttttt catctcttcc atcatttttt cagtgagcat
    16381 tacgcactga aaccccatgt ttttgatgtc tacgggtttc atgcctttgt cagtggatat
    16441 cacaggcaga atatcctgat acacatcgcg gtcagggtaa ctaagcacac aaagctccca
    16501 tgatgccacg ggtgaggatc cttcgtctgt ttcaacccac cagcctggtg tagctgcgct
    16561 tatttgcaga atcgtgatcc gtccgttgtg cttgtaaaat ttttcaccaa attttggttc
    16621 gctcatgatt ccacctctcc ggttttaacg ttgattgttg ttacctgttc cgcttcggca
    16681 atctcccgct ctgtcagcgt ggcaaaattt gccgccgtcg tggtcatgaa tgcgcttatc
    16741 agttcgggat gtgctttcgc gtatccttcc cccgcgtggc ggtctatcgt tctgattgcc
    16801 acctttaaag cgtgctctgt catgtctaac gcgcgatatt tcggttctgt tctgtctctg
    16861 cgttttttga atgatttatt aaatttccct gaagtgtgca tatttatttt taccccctcg
    16921 tttaaaaagt tttgagttgt gcctcccctt gtctacctta tctaccttag tggccctcat
    16981 gccagtaatg gcgctgcttt cagcggggta gagtgctttt atccactatc taccccgtgt
    17041 ctacctccct gtctgattca ggtaaaatca ggtagagagg gtagatagtg ggtagacagt
    17101 aaaaaaaggc tatctaccta acttaatgca ctgaattaaa tgtatttttc tttactcagg
    17161 tagacagggt agacagcaat tacaaaaaat tataaaaacg cgtcgcactc gtctgttgtt
    17221 attgcgttag tctgcgttac tcccttaact ttccgcgtaa tatattcatg tccgtaaact
    17281 ttcgcggctg gcttcatagc cttgccaaag tcatttacgt ttagcggttt gctcctgcct
    17341 gcgtacgcca taaacgccag atagacgcgg taaaggctgt ttctggtcgt gtacttcact
    17401 gaatcgccac cgccgcccat catcaggccg cgtgcttcct ccagaaaatt caggaactgg
    17461 caaaactcaa taaccggatc cgtctgttgc tttattgcca gagcttcatc accgtcacgc
    17521 tgttccagta gtaaagcccg tgctttctca gagtcggtaa agttcgccag caatcggcgg
    17581 ataatgacag ggatttcagc cgcaatcttt tccggtagct ccctgtcttt ttcggcctca
    17641 ctaacgatat tgtcgaaacg gaaaatcacg cgacgacgtg ccacacctcc ggcccgttcg
    17701 gtgaatatca tcgggttatt gttggtcgcc agcaccaccg ccctgattac agccgtgaaa
    17761 cgcttttcgt atttcgggtt aatttccacg gggtcaccgc ccgtgatttt cttgatgccc
    17821 gttccttcgc ctgtatattt cggctggtca gccaggacga taagacgact cccgacaacc
    17881 tgcgcacgtc caccagcatc atcaagcgat gtcatttcag cgcttaccgt gttctgtttc
    17941 cctgccagaa ggctggctat gtgtgtgaat gtacttttac cgctcccgcc gtctccggtg
    18001 gcctcaataa acatctgcca gtcgtaccgg ttcgccataa tcatgtacag cgcggcacat
    18061 atacgcatca tcttgcgctg gtcttttccg gctgcgtgct caagccattt atgaaagttt
    18121 agcgcgttat cgcggatgtt ctcccctggt gctggtggcg tgtactcaat gccgttgtgc
    18181 gtggtgatcc agttctccgg cgtgtgcggg gaaaattccc ccgttttcag gtcaagcgca
    18241 ccattggcga acggcagcaa atcgccggac ggctcgccca ttggttcggc aataactttt
    18301 aacgcttcca cggcgttatt gattacgcgc ttgctgaaag tggccctgtg ctctgaatag
    18361 atcaccacca tttcgcggct aagttccatt gtgctgaccg gacaccatac cccgccgcgc
    18421 catacgtgaa cgatttcact ttcctgatgc acacaaacgc catcaaagcg ctcggcaagc
    18481 agctgcgcgc gctcactgtc tgccatttgt gccagttgcg tatttttctg ctccggcatg
    18541 gtaagccctg cggcaatatt ttcacgctca gcattcagat agcgccgcca gttttcacac
    18601 ttctggacgt gcattccttc ggggtaaaaa tttgcgtcct gaatatctgc cgccgccagc
    18661 ttctgaccaa tctttttggt ctccaccaaa tccagttctc cggcctggta cagccttacg
    18721 cgtttttttc catccggaac aattttcagt gcatcaagtt cggcaagctg atttggccca
    18781 agccacacag gcggcacatt atcgccggat gcggggccat cctgctcctg ccattgtttc
    18841 gcgtgcgacc aggcatcact acccgcaaag ataattacct ctgtgtcttt gtgttttatt
    18901 ccgcgtggct gtttttttac gttcggtgcc agtttcattt tttacccctg aatacgttaa
    18961 gcatcttttt tatttcctga atattggcgc gtgctttctc cctgctggtt ggtttactgc
    19021 ggggcgctgc ctgtaccagc gtgaaatcac gccggaattg ataaacaggc atcacgcaat
    19081 catattcgta accttcacgg cggtaggtga tgcgccgttc tgccacgcct ttaatcgtta
    19141 ccatgccgcc gtactggtcg cggtaaatat caccgcgcgt aaatttaggg tgagtgttgc
    19201 cactggcagt taagccagaa tatttaagtt tcattatttt tattctccgg tgtgttgttc
    19261 tttatatctg tcgtgcaata gatctatttc ttgcagttcc attattacag gctcaagaag
    19321 cgttattaat gccgtggcaa ttcttgattt ttgtttgtcg cgttcattgt cgccaagtgt
    19381 ttcaagccat atgcgcaata tttccagcat gttttcactg tgagaaagtg caagaaatgc
    19441 gcggtctatt gtttcgtggt aaatatcacg catattaatc cccgtccgtc gtttttctta
    19501 aaataacctc tgtcacgaaa tcagcataat cggcggcgat attcagtaca tcaagcccgt
    19561 tgatttatat tctctcgtgg aaagtaagaa aaaagccgct ctaataagtt ctggcattga
    19621 cgaaagcgca tcagccgcat catcaggaac gccggaaaat tcctgtttca gggaattaaa
    19681 acgatcatca cgcataaccc cccccatttt cacaatcggc aacaagaata tttttagcgt
    19741 cattcagtga ccgcgttgcg atgtatcgga tacattccag ggcgaactgt gtccggtctt
    19801 tctgttcttc tccctgcgca agctctgccg tgcattcaat atcaataagc gcgtgcatca
    19861 gcgtagtgag tgcggcggct gctgcgttcg gtgtggtttt attgtgcata ggtttccccc
    19921 tgacgaatac gggcggcgaa tacagcaaca caaccggacg ggcaacggct acgcgcttcg
    19981 cgttccgtcc aagcggttac gtggatgatt tgggattctc cggcactcag tgccagaaaa
    20041 cgccacacaa aggccgtttg tgtgtgtaca aggtgtggta tatgatttac ggcaaccata
    20101 acggctccta gtttacgttg ttggttagaa gccccgttac tgctcctaca gtgcggggct
    20161 ttgctttttg tgttgattgt gtgtaatgtg tcactacaca taaatacatt acctgaggtg
    20221 taattaacgt gtcaacacac aaaaacgaaa ggcgtggtaa tcctccgttc cagttccgtt
    20281 tagatcctga cttacgggaa aagatggaaa aggcacaaca acaggacggc gatgattctc
    20341 ttgctgcctg gataaagcga ataatccgca aagaattaca gcaacgcggc atagaaccca
    20401 aaggataaaa actatcagcg ccgtggtgtg aggtactacg gcgcattgct ttacaggcgc
    20461 acacaatgac caacgaagaa tcaaccgaaa aaacatcacc tgcacgaaaa agacgacgca
    20521 gaaagaaaga gcatgaatca gaacgattcg cgccgtgttc ttttgccctt gagaagtacc
    20581 ttaaagaata ctcatcaaag gagagaactt cgcagttatg gcaacgcact gaaccagact
    20641 gattgcattg cccaccagcc tgatagcggc tatcatcccc gtgcttatgt ttgggatcac
    20701 acacacaaac ggcgcagcgg gttatctgtt cagaaaggcg gctccatttc ggggccgctt
    20761 tttttatgcc tgaaaaaccc caattttgtg gttttccagt ttcaccaggg cgaacgaatc
    20821 cccgcccacg ttctggcgta tattcaatct tcatggttat agctctgtgt tcagatgatt
    20881 gatgtgtggc ggctgcgtgc cgccagcgtg attaatgaac tgccttgcag ctatccttcc
    20941 aggccagaac ctcggataaa gaccagccaa cggaacgacc gccaagttta cgacgtgatg
    21001 ggaattgtcc ggccttttcc aggcggtagc ggcatgagcg gctaaggcct gttagctttt
    21061 cgcattcttt ttcacgtata aaccgatcag tgcttaacac tattgccccc tttcgtttct
    21121 taaagagtta tttcgtgttc tattgcgttg ggatgtgtct gattgtgtca ggatgattca
    21181 gagttggcaa atgttgaggc cgtatggttt acagaaagag gaataatcag gataaaatcc
    21241 tttaaattca tgttaataca aaggcataaa atattgtttc atgccttttt tctcgctatt
    21301 taaagagtga ttcgctagtg tataaaaaat cagtaacaca ttaaaaatca gctacttata
    21361 aatctgtaca ctttttcacc tcttgttcgt agttgttccg cattgttgct cattgttgca
    21421 cgttgtatct gttcgcatat ccagtatgcg catactgaaa aaacacgaaa aaaattattt
    21481 tcttctggct actggtagcg tggttacgtt ttcatgtgtt cccgccagta tccctaaccg
    21541 ctccgtccac atatccagcg cattgcgttt agcatccaga taacgggaat gattataaac
    21601 tcgctgcatt cctggcatct ggtggcctgt aagctgctcc acgacgtgag gatccacgcc
    21661 taaatcgttc agcatcgttg taaaggtgcg ccggatgtca tgtagtgacc agtgagggtg
    21721 atttagcctc ctgtgcgcta atctgccgta ctgcgacacg cttgtttcct gtttcacttc
    21781 ccccagcaat aagcccgtgt gcctgttctg ctccaccagc tgcgtgacga acggcagtat
    21841 tgcttccggt atgggccgga atattgccac cttcgttttg ctgtgttctt tcggcacggt
    21901 ccagagcatt tcggtaaaat cccactcgct gatctccgat aacctcagtt ctaccgtccg
    21961 gcatccgaac acaatcagga ggcggattaa cgcgatgtag tagggggaga atattttttt
    22021 gtccagtgcc tgcaataatt cgcccagttc tttggtgctt aagacacgct cgcttatatc
    22081 cggttttttc ccaacgtccg ccacactcat atcatcaaga acgttgctga ttgcatagcg
    22141 ccgcctccgg cagaacttaa gcgcctgttt gcacgtctgt agcaagaatc cggcagtaac
    22201 aggcgttcgc tttgccacct ggtcaaaaca ggccagccag tgccgtagct cgcatttatc
    22261 cagcggcata gcaccaatgt gctgtattac gtgattatta aggcgctttt tcagggcggc
    22321 ataatccacg cggttttcct ttgcgtacga ctcaagccag taggtgagcg catcgccaac
    22381 cgttaccggc tttaacgctt cctgtacggt gtaattaagc tcatgacgtg gatttttccc
    22441 ctctgccagc catgcgcgac actgggcggc tttttccctg gctgatttca ggctcagatc
    22501 aggataattt cccagcttaa tgcgttccgg tggtgccccc cttcccgttc cggccctgta
    22561 agtgaaatac caggttaaaa ggccactggt tgaatgcctg acgctcaggt ttccaccgtc
    22621 attaagaaag gctgtttttt gggcgggtgt gccgttgatt ttcctcagct gtgtatcgct
    22681 cagtttgtta agtgctctgc tcataatttt gattccggtc atcacaatta actacaccat
    22741 taactacacc gatcgttgca caaagggcta caacgtgaaa caagctggaa caaggaaaat
    22801 cacaaatctg tttataatca aaaacataat gaacaatctg aaacattatg aaacggcaaa
    22861 aaacactaat tgataaaata tgtccatact tcacgcatta cgttaagcat ccgttataat
    22921 cggttgcaga taccagcctg tggatgctta acatggaata ccaactcact cttaactggc
    22981 ccgattttct tgaacgtcac tggcagaaac gcccggtggt gttaaaacgc ggctttaata
    23041 attttattga cccgatctct ccagacgagt tggcgggtct ggcgatggaa agcgaagttg
    23101 acagtcgact ggtcagtcac caggatggca aatggcaggt cagccacggc ccgttcgaaa
    23161 gctacgatca tctcggtgaa accaactggt cattactggt acaggcagta aaccactggc
    23221 atgagccgac cgccgcgctg atgcgaccgt tccgtgaact accggactgg cgtattgatg
    23281 atctgatgat ttctttttct gtacctggcg gcggcgtcgg cccgcatctc gatcagtacg
    23341 acgtgtttat cattcagggt accggacgtc gtcgctggcg agtgggcgaa aagctgcaaa
    23401 tgaaacagca ctgcccacat ccggatctgt tacaggtcga tccgttcgaa gccatcatcg
    23461 atgaagagct ggagcctggt gatattcttt atattccgcc aggattcccg catgaaggct
    23521 acgcgctgga aaatgcgatg aactattccg tgggttttcg cgcgccaaat acgcgggaat
    23581 taattagcgg atttgccgat tatgtgctgc aacgtgaact gggcggcaac tactacagcg
    23641 atccggatgt tccacctcgt gctcatcctg cggatgttct gccgcaagag atggataaac
    23701 tgcgtgagat gatgctcgaa ttgatcaacc agccggaaca ctttaagcaa tggtttggcg
    23761 agtttatatc ccagtcacgt catgaactgg atatcgcgcc gccggaaccg ccttatcagc
    23821 cagatgaaat ctacgatgcg ctgaaacaag gtgatgtgct ggtgcgcctg ggtggtctgc
    23881 gcgtattgcg cattggcgac gacgtgtatg ccaatggtga gaagatcgat tccccgcacc
    23941 gtccggcact ggatgcactc gccagcaaca ttgcgctgac tgcggagaat tttggcgatg
    24001 cgctggaaga tccgtcattc ctcgcgatgc tcgcggcgct ggtcaatagc gggtattggt
    24061 tcttcgaagg gtaagtttgg atttaagccg gatgcagcat cacatgccgc atcccttttc
    24121 gcttacttcc gttgcgccgt taactcggca atacggacga tcacctgcac cgctttttcc
    24181 ataccttcca gaatcacaaa ctcatgctta ccatgatagt tgtaaccgcc agtgaacagg
    24241 ttcgggcaag gtaatcccat aaacgacaac tgcgcgccgt cggtaccgcc gcggatcggt
    24301 ttcagttccg gttcaatatc gcagtcacgc atcgcctgct gggcgatatc gagaatatgc
    24361 ggatgctcaa ccactttctc gcgcatattg tagtaactgt cttcaatcac caattcaatg
    24421 tagcaatcag gatgtaaccc tttgcccact tttttggcga tctccatcat tttacgttta
    24481 cgcgcttcaa actgtttacg gtcgaaatca cggatgatgt agtgcatatc ggcccgttca
    24541 acggtgcctt tcatgctcgc caggtgatag aaaccttcat agccttctgt catttccggg
    24601 ctttcatccg ccggaacttc cgcatgaata cgtgccgcca gcgacagcgc atttaccatc
    24661 actcctttcg ccgtgcccgg atgaacattg ttaccgacaa tttttatatt gaccgatgcg
    24721 gcgttgaagt tttcaaactc cagttcgcct acgccaccac cgtcaacagt gtaagcccag
    24781 cgggcatcga aggcatcaac atcaaaatgt ttcgcccctt tgcccacttc ttcatccggg
    24841 gtaaaggcga cgcgaatatc accatgcgga atgtttttct gttgcaatac cgccagcgcg
    24901 gtcatgattt ctgcaatacc tgctttgtca tcagcaccta acaaggtttt accatcggtg
    24961 gtaatcaacg tctgacccag tagctgatgc agcaccggga acataaccgg tgataaaact
    25021 tcatcgccga tacccagcgc aatatcgcca ccgcgatagt tttcaacaat ttgcggatta
    25081 acatttttgc cgctgcaatc cggtgaggta tccacatgag aaataaagcc aatcgccggg
    25141 atatcgccag ggacgttagc cggtaacgtc gccatcaaag tgcccttctc acttaaggtc
    25201 acattgataa gccccatttc ttcgagctgc tctttcagca gatgcaataa cttccattgg
    25261 ccttccgtgc tgggaacctg tctcacccct gcttttgatt gggtatccag agacacgtag
    25321 ttcaaaaatc gctcaagtag tttatccatg tagtcaccct cactttttgt gacaacatta
    25381 ttaagaagca agaaaagaca aatattgcgt caggtcactt ttacccctgc aagcgggaat
    25441 atttatcagc attaccttaa tgaatataaa gctaagccag taattcacaa caaagattgg
    25501 cgattcaagc ggtttgccgt agaattaccg ccctcattag gagtcaccaa ggtggttaac
    25561 cacaaacccc gcatcggtaa gccatccgtt gcgtttacat gggacagagt aaaaaattga
    25621 ataaacaacc gagttcgctt tcaccgctgg tgcaattggc gggaattcgc aaatgctttg
    25681 atggtaaaga ggtcattccc cagctggatc tgactatcaa caatggcgag ttcctcacgc
    25741 tgcttggccc ttctggctgc ggtaaaacaa ccgttcttcg cctgattgca ggtctggaaa
    25801 ctgttgattc cggacgcatc atgctggata acgaggacat cacccacgtt ccggcggaaa
    25861 accgctatgt gaacactgtt ttccaaagct atgcactttt cccccacatg accgtgttcg
    25921 aaaatgtggc ctttgggttg cgcatgcaaa aaacccccgc tgctgaaatt acgccccgcg
    25981 tgatggaagc cctgcggatg gtgcagctgg aaaccttcgc tcaacgtaaa ccgcatcagc
    26041 tctctggtgg tcaacagcaa cgcgtcgcca ttgctcgagc ggtggttaac aagcctcgtc
    26101 tgttgttgct ggatgagtcg ctctcagcgc tggattacaa actgcgtaag caaatgcaga
    26161 acgagctgaa agcgttacag cgtaagcttg gcattacatt cgttttcgtg actcacgacc
    26221 aggaagaagc actcaccatg tcggacagga ttgtggtgat gcgcgatggt cgcattgagc
    26281 aagacggcac gccgcgtgaa atctacgaag agccgaaaaa cctgtttgtt gccgggttca
    26341 ttggcgaaat taatatgttt aacgccacgg tgatcgagcg actcgacgag cagcgcgtac
    26401 gcgccaacgt tgaaggccgc gaatgtaata tctacgttaa cttcgccgtt gaaccggggc
    26461 aaaaactgca tgttctgctg cgcccggaag acttacgtgt tgaagagatt aacgacgaca
    26521 accacgctga agggctgatt ggttacgttc gtgagcgtaa ctacaaaggc atgacgctgg
    26581 agtcggttgt tgaactggaa aatggcaaga tggtgatggt cagcgaattc ttcaatgaag
    26641 acgatcctga ctttgaccac tctctcgacc aaaaaatggc cattaattgg gtagaaagct
    26701 gggaggtcgt actggctgat gaagaacaca agtaagttcc agaatgtagt gattgtcact
    26761 attgtcggtt ggcttgtgtt gtttgtcttt ctgcccaacc tgatgatcat tggcaccagc
    26821 tttttgaccc gcgacgacgc cagtttcgtc aaaatggtct ttacactgga taactacacg
    26881 cgtctgctcg atccgctcta ttttgaagtg ctattgcact cgctgaatat ggcgctgatc
    26941 gccaccctcg cctgcctggt gctgggctat ccatttgcct ggtttctggc gaagctgcca
    27001 cacaaggtgc gtccgctgct gctgtttctg ctgattgttc cgttctggac caactcattg
    27061 attcgtatct acgggctgaa aattttcctc agcaccaaag gctatctcaa cgagtttttg
    27121 ctctggctgg gcgttatcga cacaccaatc cgtatcatgt tcacgcccag tgcggtgatt
    27181 atcggtctgg tttacattct gctgccgttt atggtgatgc cgctgtactc cagtatcgaa
    27241 aaactggata agccgttgct tgaggcggcg cgcgatctcg gtgccagcaa gttacagact
    27301 tttatccgca tcattattcc actgacgatg ccggggatta ttgccggatg tctgctggtg
    27361 atgctgccag cgatgggcct gttctatgtg tccgacctga tgggcggtgc gaaaaacctg
    27421 ctgatcggta acgtcatcaa ggtacagttc ctcaatattc gtgactggcc gtttggtgca
    27481 gccaccagca ttacgctgac tatcgtaatg ggcctgatgt tgctggttta ctggcgcgct
    27541 tctcgtttgc tgaataagaa ggtggaactc gaatgatcgg tcgactgctt cgcggcggtt
    27601 ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt gtgaactcct
    27661 ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg tatagcctgc
    27721 tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg gcggtgtttt
    27781 cggcgacgtt tgctacgctt atcggttcac tgacggcagt tgcgctgtac cgttatcgct
    27841 ttcgcggtaa gccgttcgtt agcggaatgc tgtttgtggt aatgatgtca ccagatatcg
    27901 tgatggcaat ttcgctgctg gtgctgttta tgctgctggg tattcagctt ggcttctggt
    27961 cgctgctgtt ctcgcatatc accttctgcc tgccatttgt ggtggtgacg gtgtattcgc
    28021 gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt gccagcgaat
    28081 ttaccattct gcgaaaaatc attctgccac tggcaatgcc agcggtggcg gcgggctggg
    28141 tgttaagctt taccctgtcg atggacgatg tggtagtttc ttcattcgtc accggaccaa
    28201 gttatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta tcgccggaag
    28261 ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggttatt gccagccagc
    28321 ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa aatggtcacg
    28381 ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg ccgatgacaa
    28441 caacacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc ttgaacagtt
    28501 caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg aaaccatgta
    28561 cgccaagctg aaaacgtaca aagacggtgc ctatgacctg gtggttcctt caacctatta
    28621 cgtcgataaa atgcgtaaag aggggatgat ccagaagatc gacaagtcga agttaacgaa
    28681 cttcagcaat ctcgatccag acatgctcaa caagcctttt gacccgaata acgactactc
    28741 cattccgtat atctggggtg cgacggcgat tggtgttaac ggtgatgcgg tggatccgaa
    28801 atctgtcacc agctgggccg atctgtggaa gccagagtac aaaggcagcc tgctgttgac
    28861 cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag ctgggctact ccggtaacac
    28921 caccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga tgccaaacgt
    28981 cgcagcgttt aactccgata acccggctaa cccgtacatg gaaggcgaag ttaacctcgg
    29041 catgatctgg aacggttctg ctttcgttgc acgccaggcg ggtacgccta ttgacgtggt
    29101 gtggccgaaa gaaggcggca tattctggat ggacagcctg gcgatcccgg caaatgccaa
    29161 aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg tggcaaaaca
    29221 ggttgctgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc tgttaagtcc
    29281 agaagtggca aacgataaga cgctctaccc ggatgctgaa accattaaaa atggcgaatg
    29341 gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga agctgaaagc
    29401 aggacgttaa ttcagcaagc gggcggtggt attaccgccc gttttttagt tagcaaggat
    29461 gtggctaatg agtcaggaca gtaaagtttt atcccgctct ttcgtcggta ttgtggtccg
    29521 cttgtaggcc tgataagcgt agcgcatcag gcaatgcttc ctgctttata atccctccag
    29581 caacttttca acaaattctg gcaccacctg gcttgccggg ccgtaatatt tctcggcaaa
    29641 ttcattacca acctgactcg gttcaagatt cagttcaacg gtgtgagcac catgtagttt
    29701 ggcttcgtgt acaaacccag ccgccggata gacatgcccg gaagtaccaa tggcaataaa
    29761 gacatccgcc atcgacaacg ccatataaat ttcatccatg ccgagtggca tttcgccaaa
    29821 ccaaacgacg tgcgggcgca agggtgccgg gaactggcaa caatggcatt tatcttctgg
    29881 cgtaacgtct ccggtccagt cgagaacctg accactttgc gaacaacgaa ctttcagcaa
    29941 ttccccatgc atatgaatga cattggtatt acctgcgcgt tcatgcaggt tgtctatatt
    30001 ctgcgttacc agcaaaaagc gatcgccgag agcgtcttgc agtttagcca gcgcaagatg
    30061 cgcggcgtta ggctgaattt ctggctgctg cagctgtcga cgacgggcgt tataaaacgc
    30121 ttgcaccagt tcaggatcgc gatcgaaacc ttccggcgtt gccacatctt caacccgatg
    30181 ttcttcccac aggccatctg cggcgcgaaa ggtacgaata cctgattccg cagaaattcc
    30241 tgcccctgtc agtacgagta ctcttggttt ttccattgct tccggcacca ctttatctct
    30301 gaaaaaaata cgctgacgca aacgctcgcg caggcgacgt ttatttttac gaaaacgact
    30361 taaccgatga ccccgacgcg acagcatagc aacctctgtt gtttaatcgg ttagatgtag
    30421 gaaggccgca ccacgcattc ctcccgcatc accgtggcgc gcgcgttcaa tgcgtggaac
    30481 acgagctaca ggtaagagat gacaaggcaa cctgtccgcc agttgcgttg tgattgccgg
    30541 gaaattcgat aagccaccac caatgacgac caggtcaggg tcaacaatgg tcaggatatt
    30601 tcccagagaa accgctaata aatccagata acgctcaacg tgcgcccttg cctgctcatc
    30661 gccttgatca taaagcgcaa taatttcggg agcctgcaac ggttgatgat aatagtgttg
    30721 atacagccac gcaaaaccgc gaccagacag ataattttca atgcagccat gctgaccaca
    30781 gccgcagcgg cgtaacggga aatccaaccc catcatggtt aacgcatcaa ccggcagacg
    30841 catatggcca aactcgccgg taatgtagct tttaccagta atcggtttgc cgttgaaaac
    30901 cagcccgccg ccaacgccgg tgccgagaat taaccccatc accagtggat attgcgtaaa
    30961 ttcatcatcc caggcttctg aaagggcaaa acagttggca tcgttatcaa ggcgtacatc
    31021 gcgatcaaga cgtgcgctca ggtcggcacg cagcggtttg ccgctggcag cggggacatt
    31081 agcggcatac agcttaccat cttctgtttc cggcattccc ggaataccga tgccgacaga
    31141 acctttacag ccaaaacgtt ggtcagcttc agctaccagc tcacacactg catctaaaaa
    31201 tgcgtcatag ctgtcacgcg gtgtcggcac ccgcttttcc cactgcaact gccgaccgct
    31261 atcaaacacg ccaagcgcaa tttttgttcc accaatatca aacccgtaat acattgccgc
    31321 tccttgtttt aatgtactgc ctttactggc cgctaaggac tcgcgcaggg tcaatattac
    31381 tggcgcgccg cgccggatac caacttgcca aaagactcag caacaatgct gtgaccagta
    31441 cgtagaagac gtccagccag tgcaattccg atggcaggaa gtcaataaaa taaatatcgc
    31501 tggagaggaa ctgatgaccg atcagctttt caatccactc aataatcggg gtaagttgca
    31561 gtgaaacaac tacgccgata accaccccac acaggctgcc gaacagccct gccagcaatc
    31621 cataccagac aaagatggcg cgaattaaac catctttcgc ccccagcgtt cttaatactg
    31681 cgatatcgcc actcttgtct ttcaccgcca tcactaaggt ggagacgatg ttgaaacagg
    31741 ccacgccaat caccagtacc atcgccagat acataatggc gcggatcatc tggatatcgc
    31801 gatacatata gccgtaagta ccaatccaac ttttaatata aacatagctg ttggtcactt
    31861 cacccgcatc gcgtaccagc ttattggcgt tgaaaacatc cgtcatttta agggcaatac
    31921 ctgacacgct ggaacccata tcaagatatt gttgggcatc cgccagcggg atcatggcaa
    31981 aactgtgatc gagttgacca ctcaactgca aaataccggc aatgtgcaaa cgcacacgtt
    32041 ttggctgcat cagtttatgc tcgggattcg agttggggat catgatcgac acccaatcgc
    32101 cctgcttcac tttcagcgca tccgccacgc ctttgccgat gataatttgc tgttcgcccg
    32161 ctttaaaatt gcgccacgca tccccctgaa caaacgaggg taatgcgctc agacgctgtt
    32221 cctgttgtgg gttaacgccc ttcacctgga ttgcgcgcag attcgctcca ctttccacca
    32281 gaccggtgaa attgatatac ggcgcagcgg cggcaatacc cggcactttc tgcacgttat
    32341 ccagtgcttc ctgccagtta gtccatggct gatttaccgc ttcgatttcg ccatgcggca
    32401 ccaccgccag aatgcggtta ttcagttcgc gttcaaagcc gttcatcgcg cttaagccga
    32461 cgatcaatac cgccacgccg agggcaatgc caatggtaga aatgacagag attagcgaca
    32521 ccatgccgcc gcgtcgccgt ccgcgactaa aacgcaggcc aatcaataac gataaaggca
    32581 tcgccattac tccgccccca tcaggctcag ttccgccgtc agacgtccat cacgcatttc
    32641 cagttggcgg ctcatacgtt tcgccagttg caggtcgtga gtgaccacca ggaaagcggt
    32701 gccctgcaag cgattcaact ccccaagcaa ctggaagatg ctgtcggcgt tacgcgcatc
    32761 gaggttaccg gtaggttcat ccgccagtac cagacgcgga ttattgacca gcgcacgggc
    32821 aatcgctaca cgctggcgtt cgccgccaga aagttcagat gggcggtgat tcgcacgatg
    32881 ctccagcccc accgctttta acatctccag tgcacagctg ttgatttcag cgggcttttt
    32941 cttgccaatc agcagtggca tagccacgtt ttccagggca gtaaaatccg gcagcaggtg
    33001 gtgaaactga taaataaagc ccagcttctg gttgcgcagt tctgctttcg ccgctgaaga
    33061 cagtttgctc atcggctgac cgttaaagat cacatcgccg gaggttggcg tatccagccc
    33121 gcctaacagg tgcagcaagg tacttttacc ggaaccagag ctaccgacaa ttgccatcat
    33181 ttcaccttcg cccacgctga aactgacatt gagcagcaca tcggtttgca cactgccttc
    33241 ctgatagcgt ttgcacaggt tgtcgcattg caacaggatc ttattcataa cgtaaagcct
    33301 cagcgggttg tgtggcggca gcgcgccatg aagggtaaag cgtagacagc agcgcgatag
    33361 ccatcgccac cagcgcaata acaatgacct gtaaaggttc gatagccacc ggcagcgccg
    33421 cgccatcaag caggacgccg attatcggca tcagattatt taactggctg gcaagcagtg
    33481 cgccaagcgc cgctccgagg attgcaccga taatcccggc gctagcccct tgcaccataa
    33541 agaccatcat gatttgtcgc ggagttaagc cttgcgtttg caggatcgcg acttcgccct
    33601 gcttctccat caccatcagc cccagcgagg taataatgtt aaacgccgca acagcgacaa
    33661 tcaggctcag cagtaagccc atcatatttt tttccatgcg tacggcctgg aacagctcgc
    33721 ctttacggtc acgccagtcc tgccatttgc tgccttcagg cagtttttgc tgacttaaag
    33781 agtcaactct cagcggctca tccagccaca aacgccagcc ggtaatattg gctgccggat
    33841 aacgcatcag acgcgaggca tcctcaatat tcaccagcat ttcatagcca tcgacttcac
    33901 tgttggcggc gaaagtgcca atcacattga acaggcgctg acttggaata cgccccatcg
    33961 gcgtgaactg gctggcagat ggcaccatca cgcggatttg atcaccgcga ttgacgccca
    34021 actgtgaggc aagttgttcg ccgaggatga cattatattt ccccggctcg aggtcagttt
    34081 gtttcacatt gaccagatac ggcgttagtg gatctttttg cgccgggtcg ataccgagca
    34141 tcaccccgac cgccacgctg cgcgcgcttt gcagtaccac atcaccggta gtaataggtg
    34201 cgacgcgatt aacgccgtcc agtttgactg ccgtttccgg gagttgctgc gggttaagag
    34261 agccatgctc agaagagaga attgcctgtg gcatcaggcc aaggatgttg ttttgcagct
    34321 cgcgctcaaa gccgttcatc actgacaata ctgtgaccag cgccatcacc ccgagggtaa
    34381 tgccgatggt agaaagccag gagacgaaac gaccgaagcg atccactgca cgcccacgca
    34441 tgtaacgcag gccaataaat agagcgacag gttggtacat gaaatccgtc tggttgctgt
    34501 agcaaagaca cgagtatata agcgaaagca agatgggtaa acgtctgaac tgtaagtgat
    34561 cgtcagggct aatctgaaaa gcccccgtaa atcaatcggc taaattgcca tattttatca
    34621 tcaatcttgc cgcctgatct taatctgaaa aatcatccaa tacagacaga tacccattac
    34681 acctcgggtc ataaccagca ggatcaaccg catgtaaatt aaaaaagagc gtgttgttct
    34741 gatgaaacaa aaagagctat ggattaacca gatcaaaggg ttatgtattt gtctggtggt
    34801 gatttatcac tcggtcatta ccttttatcc gcatatgacc acttttcagc atccgttatc
    34861 ggaagtcctg agcaaatgct ggatctattt caatctttac cttgccccct ttcgtatgcc
    34921 ggtttttttc tttatatccg gctatttgat tcgccgctat atcgacagcg taccgtgggg
    34981 aaattgtctc gataaacgga tctggaacat cttctgggtg ctggcgcttt ggggcgtggt
    35041 gcagtggctg gcgttaagtg cgctgaacca gtggctggca cctgagcgcg atttaagcaa
    35101 tgcctccaat gccgcttatg ccgattccac cggtgagttc ctgcacggga tgatcaccgc
    35161 cagcaccagc ttgtggtatc tgtatgcgtt aattgtctat ttcgtggtat gtaaaatttt
    35221 tagccgtctg gcgctgccac tattcgcctt gtttgtactg ctgagtgtgg cggttaattt
    35281 cgttcccacg ccgtggtggg gaatgaacag tgtgatacgc aatttgcctt attacagcct
    35341 tggcgcatgg tttggcgcaa caataatgac ctgtgttaaa gaggtgccgt tgcgccgcca
    35401 tctgctgatg gcttctttgc ttaccgttct ggcggtcggt gcctggttgt ttactatctc
    35461 gctgctgttg tcgctggtat cgattgtggt gatcatgaag ctgttttatc agtacgagca
    35521 acgattcggt atgcgctcca ccagcctgct gaatgtgatt ggctccaaca ccattgctat
    35581 ctacaccacc catcgcattc tggttgaaat attcagctta actctgcttg cgcaaatgaa
    35641 cgcagcacgc tggtcgccgc aagtcgaact gacactcctg ctggtttatc cctttgtcag
    35701 tttgttcatc tgtactgttg cgggcttgct ggtaagaaaa ctttcacagc gtgcattcag
    35761 cgatctgttg ttctccccgc cttcactgcc cgctgccgtc agttactctc gttaaaccat
    35821 caccccagcc ggggcgattc aatttgctaa accatgtcga ttacggataa tgaacagcat
    35881 tgcttatcag agatatgccc ccatatgttg aggcatatcc taacgagaat ctgacaaccg
    35941 ttatgcctga acaatatcgt tatacgctgc ccgtcaaagc gggtgagcag cgtctgctgg
    36001 gcgagttaac cggcgcagcc tgtgcaacgc tggtagcgga aattgccgaa cgtcacgccg
    36061 gtccggtggt actcattgca ccagatatgc aaaatgctct gcgtttgcat gatgaaatca
    36121 gccagttcac cgatcagatg gtgatgaatc tggcggactg ggaaactctt ccctacgaca
    36181 gtttttcgcc tcatcaggac attatctcct cgcgcctttc caccctttac cagctaccga
    36241 cgatgcagcg tggcatactg attgttccgg tgaatacgct tatgcagcgc gtttgcccac
    36301 acagttttct ccacggtcat gcgctggtga tgaaaaaagg tcagcgcctg tcacgagatg
    36361 cattacgaac ccaactggac agcgccggtt atcgccatgt tgaccaggtg atggagcacg
    36421 gcgaatacgc cacgcgcggc gcgttgctgg atctcttccc gatggggagt gagctgcctt
    36481 atcgtcttga tttctttgat gatgaaatcg acagcctgcg ggtgtttgac gtcgacagcc
    36541 agcgcacgct ggaggaagta gaagcgatca atctgctgcc cgcgcacgaa tttccgaccg
    36601 ataaagcggc aattgaactg ttccgcagcc agtggcgcga taccttcgaa gtgaagcgcg
    36661 atccggaaca tatttaccag caagtgagta aaggcacatt acctgctggg atcgagtact
    36721 ggcagccatt gttcttcagc gaaccactgc caccgctgtt cagttatttc cctgccaata
    36781 ccttgctcgt gaatactggc gatctggaaa ccagtgccga acgtttccag gctgacacgc
    36841 tggcgcgttt tgagaatcgc ggcgtcgatc cgatgcgccc gctgttgcca ccacaatcgc
    36901 tctggctgcg ggtggacgag ctcttctcag agctgaaaaa ctggccccga gtgcagctaa
    36961 aaactgaaca tttaccgaca aaagccgcga atgccaattt aggtttccag aaactgccag
    37021 acctggccgt tcaggcgcaa caaaaagcgc cgctggatgc gctgcgtaag ttcctcgaga
    37081 ctttcgacgg tccggtggtg ttctcggtag aaagtgaagg tcgccgtgaa gcgctgggtg
    37141 aactgctcgc acgaattaaa attgctccgc aacgcattat gcgtcttgat gaagccagcg
    37201 accgtgggcg ttatctgatg attggcgctg ccgaacatgg ttttgtcgat aaggcgcgta
    37261 atctggcgct gatctgcgaa agcgatctgc tcggtgaacg tgttgcccgc cgtcgtcagg
    37321 attctcgccg caccatcaac cccgatacac tgatccgtaa ccttgcggag ctgcatattg
    37381 gtcagccggt ggtccatctg gagcacggcg tcggtcgcta tgccggaatg accacgctgg
    37441 aagctggcgg cattactggc gagtacctga tgctcaccta tgccaacgac gccaaactgt
    37501 atgttccggt gtcgtcactg catctgatta gccgttacgc aggtggcgcg gaagaaaacg
    37561 ccccgctgca taaacttggc ggcgatgcgt ggtcacgcgc gcggcagaaa gcggcggaaa
    37621 aagtgcgtga tgtggcggcg gaattgctgg atatctacgc gcaacgcgcc gccaaagagg
    37681 gcttcgcgtt taaacacgat cgtgagcagt atcagttatt ctgcgacagc ttcccgtttg
    37741 aaaccacgcc ggatcaggcg caggccatta atgcggtact tagcgacatg tgtcagccgc
    37801 tggcaatgga tcgtctggtg tgcggcgatg ttggctttgg taaaacagaa gtggcgatgc
    37861 gcgccgcttt cctggcagta gataaccaca agcaggtagc ggtgttggtg cctaccaccc
    37921 ttcttgcgca gcagcattac gacaacttcc gcgaccgttt cgccaactgg ccggtacgta
    37981 tcgaaatgat ctcccgtttc cgcagcgcca aagagcagac gcaaatcctt gcggaagtgg
    38041 cggaagggaa aatcgatatt ctgatcggta cgcacaaact gctgcaaagt gacgtcaagt
    38101 ttaaagattt aggcctgctg attgtcgatg aagaacaccg cttcggggtg cgtcataaag
    38161 agcgcattaa agcgatgcgc gcgaacgtgg atattctgac gcttactgca acgccgatcc
    38221 cacgtacgct gaatatggca atgagcggaa tgcgtgacct gtcgattatc gccacgccgc
    38281 ccgcccgtcg tctggcagtt aaaacctttg tccgtgagta tgacagcctg gtggtccggg
    38341 aggcgatcct gcgtgaaatt ttgcgcggag gacaggttta ttatctctac aatgatgtgg
    38401 aaaacattca gaaagccgcc gaacggctgg cagaactggt gcctgaagca cggattgcca
    38461 tcggtcacgg gcagatgcgc gagcgcgaac tggaacgggt gatgaatgat ttccatcatc
    38521 aacgtttcaa cgtgctggtt tgtaccacca ttatcgaaac cgggatcgac atcccgacag
    38581 ccaacaccat tatcattgaa cgtgcggatc acttcggtct ggcgcagctg caccagttac
    38641 gcggtcgcgt cggacgttca catcatcagg catatgcatg gctgctgacg ccgcatccaa
    38701 aagcgatgac taccgatgca caaaaacgtc ttgaagcgat tgcctcgctg gaagatctcg
    38761 gggcaggttt tgcgctggca acgcacgatc tggagattcg cggcgcgggt gaactgcttg
    38821 gcgaagaaca aagcggctca atggaaacca tcggtttctc gctgtatatg gagttgctgg
    38881 aaaacgccgt cgatgcactg aaagccggac gcgagccgtc gctggaagat ctcaccagcc
    38941 agcaaacaga agtcgagctg cggatgccgt cgctattgcc agatgatttc atccctgacg
    39001 tgaacacgcg tctgtcgttc tacaaacgta ttgccagcgc caaaacggaa aacgaactgg
    39061 aagagatcaa agtcgagctt atcgatcgct tcggcctgct gccagatccg gcgcgtaccc
    39121 tgctggatat tgcccgtctg cgccagcaag cgcagaaact ggggatcagg aagctggaag
    39181 gtaatgagaa aggcggggtg atcgaatttg ccgagaagaa tcacgttaat ccggcctggt
    39241 tgattggttt gctgcaaaaa cagccgcagc attaccgcct tgatggtccg acgcgcctga
    39301 aatttattca ggatttgagt gagcggaaaa cgcgtatcga atgggtacgc cagtttatgc
    39361 gtgaactgga agagaacgcg atcgcttaat gtaaatcggg ggcgtttctg cgcccccatc
    39421 tgcaacattt acaaattctt tgcactttcc tgcactatcc gacactgtca ccatccataa
    39481 ttcaggctta tctgtttatt acaataacct tatatttatt atgtattttt ggtgatgatc
    39541 aaaacgcgtt tttctcgctg gctaacgttt tttacgttcg ccgctgccgt ggcgctggcg
    39601 ctaccggcaa aagccaacac ctggccgctg ccgccagcga gcagtcgtct ggttggcgaa
    39661 aacaaatttc atgtggtgga aaatgacggt ggttctctgg aagccatcgc caaaaaatat
    39721 aacgtcggct ttctcgctct gttacaagct aaccccggcg ttgatcctta cgtaccgcgc
    39781 gcgggcagcg tgttaacgat cccgttgcaa accctacttc cagacgcgcc gcgcgaaggc
    39841 attgtgatca acattgcgga gctgcgtctc tattactacc cgccgggtaa aaattcggta
    39901 accgtgtatc caataggtat tggtcagtta ggtggtgaca cgctgacacc gacaatggtg
    39961 accaccgttt cagacaaacg tgcaaaccca acctggacgc caacggcaaa catccgcgcc
    40021 cgttataaag cacagggaat tgagttgcct gcggtagtgc cggctggacc ggataaccca
    40081 atgggccatc atgcgattcg tctggcggcc tatggcggcg tttatttgct tcacggtacg
    40141 aacgccgatt tcggcattgg catgcgggta agttctggct gtattcgtct gcgggatgac
    40201 gatatcaaaa cactctttag ccaggtcacc ccaggcacca aagtgaatat catcaacact
    40261 ccgataaaag tctctgccga accaaacggt gcgcgtctgg ttgaagtaca tcagccgctg
    40321 tcagagaaga ttgatgacga tccgcagctg ctgccaatta cgctgaatag cgcaatgcaa
    40381 tcatttaaag atgcagcaca aactgacgct gaagtgatgc aacatgtgat ggatgtccgt
    40441 tccgggatgc cggtggatgt ccgccgtcat caagtgagcc cacaaactct gtaaatcctg
    40501 gataaaaaaa atccccgcga cacggcatgt cgcggggaaa aaatcagcag tagcaggcat
    40561 taatgagggt taatgcttat ttataaatta ctgctgttcc atggagggta ttcggaccgg
    40621 ttacagaagt aatacggaaa gattttgcgc ccatctcatc cgctttttgc gccagctgct
    40681 cttccagcga tcccagattt gtccccgcgt tagcactgat agtaccgact ttttgttggc
    40741 cttctggcgt tgactgaact tcgacagccg caaagttggc aaatgacatg gagcttaaaa
    40801 tcgccgcagc gatgaggttt ttacgttttt cataatagtg gccttatgca gatgaatgac
    40861 gtcgcaagaa attacttaac gatcgataac taaatgatag atgtgatctg gatcacatac
    40921 aagatatttt ttataacaat cattaattaa tttaaaaatc tttaaatttc agtcaaatag
    40981 aaagcaatta tttttatggg atctggactg gtgaacggag gtgattattt ttataataac
    41041 ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac acaatgtgta
    41101 aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga taaggccatg
    41161 aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt cgaagcaacc
    41221 ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt atttcgtgcc
    41281 gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct gttttgtgaa
    41341 gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa ctgctttacc
    41401 agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct ctccccagat
    41461 tcaggagata tagccaatac gttgaaatca cgccatgcga tgcaagagcg cactttgcag
    41521 cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga cgtgacacat
    41581 ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg cgaaggtgca
    41641 tcgctggaaa aactgatgca gattgccgga acgactttgc gtttatggcc cgaactggtg
    41701 aaataagtcg caaaaaaggc ccctcagcct gagcgaatga ggggccaaat tgcagagtac
    41761 agccttgtta tactttgtta ttcaaaatca gttgcccgtt actatctagc gggatctgcg
    41821 tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta taggtcacat
    41881 cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg
    41941 tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat ccaccaccca
    42001 gcgcccccac aacagtggcg acatctttac cgcgaccacc accaaactga tgccctatca
    42061 cgccaccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc tgcaccggtc
    42121 gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc gtttccttga
    42181 ttggggttgc agaaacaacc tgagcgtatt gcgggccccg ttcaaacacg ttcagactgg
    42241 ccactgccgc tacgcccaac gcagctgcga caccaatccc gatacccgcc aacattgatt
    42301 tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga aggagaaaag
    42361 cagctatcag acagaagatg aaaagcgcgg gaatgactgg aaatacttaa ggattcggag
    42421 gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac
    42481 catttgtcca gggcggataa ggcgtttata ccacatccgc cagtgtacgt cgattaatgc
    42541 aacttcaaac gcggacggat aacgcggtta atactcccca ccagcatcat taatccggtt
    42601 ttaaagtaac catgcagcgc aatctgatgc attcggtata gcgagatata tacaaagcgc
    42661 gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt tacccatcag gctaccaacg
    42721 gtggagaagt tcgacagcga taccagcgaa ccatgatctt tatactgata atttttcagc
    42781 ggcttaccgt tcatctgcgc cagaatgttg ttcattgcgc aagtcgccat ctggtgtgca
    42841 gcctgagcac gcggcggaac aaagccccct tccggacgcg ggcatgacgc gcagtcgcca
    42901 atagcgtaaa tgtctggatc gcgagtggtt tgcagcgtcg gttccaccac cagctggttg
    42961 atacggttag tttcaagacc accgatatct ttcaggaagt ctggcgcttt gatcccggct
    43021 gcccatacca tcagatcagc ctcaatatat tcgccatctt tagtgtgcag gccgccttca
    43081 tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc caagtttcgt tagctcgttg
    43141 tgggccgcag cagagatacg tggcggtaat gcaggcaaaa tacgttctcc cgcttctacc
    43201 agcgttacgt tcagggcttc gttggtcagg cctttgtaac cgtagctgtg cagttgcttg
    43261 atcgcgttgt gcaattcagc ggagagttct acacccgtcg cgccgccgcc gacaatcgca
    43321 atgttcactt tgccatttgc gcccaggttg gcagagtatt tcaggaacag attcagcatc
    43381 tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga aaatgcagtt ctctttgacg
    43441 cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca tgaccagggt gtcataggcg
    43501 attttacgtt ccggaaccaa cagttcacct ttctcgtcgc gtagttctgc aatagtgatt
    43561 gttttcgctt cacgatcaat atcaatgacg gaacccaact ggaactggaa accatgattg
    43621 cgcgcatggg ccagatagct caacgcatcg acgccttcat caagcgagcc agtcgccact
    43681 tcgtgcagca gtggtttcca caggtggctg tggttacgat cgaccagcgt aattttggct
    43741 tttttcttac gtcccagctt atgccccagc tgtgttgcca tttccagccc accagcaccg
    43801 ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg tgcccccctt aaatttatta
    43861 accaattgtt aattaatggt tattaacata gctcttaatt aacaacaggt tacagcaatg
    43921 aaaatgttct tcgctattga gaataacatg aatggtgcat tggtcatacc aaaattgatg
    43981 ttaatcaagt tttgttgctt aaaagatagg caactttacc gggggagatc aaaaaataga
    44041 gtttattgtt tattatttga gcaaattcgg aagggtaata tagggtgaag cgtatatcga
    44101 gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc aggcattgat gctcgactgt
    44161 cggatgcggc gtggacgcct tatccgactt acagaattcg gagcgcatca ggtaccggca
    44221 aggttacctg atgcgacgag ggcatttacc cgagggtttt gaacgcttta atgcgctgta
    44281 aatgcgggga gatattcttg aatttgtgcg tctgctcttc gtcccagaca atctcgtaat
    44341 aatgatgcaa ctcttcagat gtccgctggc tgttaagtgc ttcatcatta cgcgacaaaa
    44401 taaccagaca acgatcgcga ttcttctcgc ggaagttggt cacacactta gtggcaatat
    44461 cggcatactc ttccgggcga tcaatcttgc cttccatgtt ctcataaggg aacaaattag
    44521 ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat ccgttccgcc cagtatccgc
    44581 ccaggccaac gccgcaaatt agcggacgct cgtcaacgtt cagttgcaac attttgtcca
    44641 cttctttaag cagatgctgc atatcatgtt tcggatgccg cgtactgtag cttatcaagc
    44701 gtacatccgg gtcaataaac tgcaattgta agactttctc gtggttaccc ggactgttag
    44761 agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca taagccaggg ttagtgacct
    44821 actttctctt cctgccagcg ttcatgtaac tgattcagac gggtgctgat cgctttccag
    44881 cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt tatgatacaa acgtgtaaca
    44941 cgttctgcct tgatcggtga cagattatct aacacgctga cggccccttt acgattattg
    45001 cagaccagga tcatatcgca acccgcatcc agtgatgcct gcccgcgttc ggcataactg
    45061 cccataatcg cggcaccttc catcgataaa tcgtcagaga aaatcacgcc gtcaaacccc
    45121 agttcctgac gcaaaacggt tttcagccag tagggagaac cgctcgccgg acgcggatca
    45181 acatcactgt agatcacatg cgcaggcata atggcgtcga gtttattttc gcggattaag
    45241 gaactgaaga ccgacatatc tttagcgcga atctccgctt gtggacgtgg atcgcacggt
    45301 gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg ggaagtgttt cccggtcgtt
    45361 ttcattccgg cttcatgcat accatcaata aaccggctgg caattgccag ggctttttgt
    45421 ggatcggcat gataagaacg ctcgccaatc gccgcgctga tatgcccgac atccagcact
    45481 ggcgcaaagc tgatatcaat atccatagcg atcatttcgc tggccatcag ccaacccgcc
    45541 tcttgcgcca gtttgccgcc ctcttccatt cccaacagcg cagcaaagga ttgtgctgcc
    45601 ggtaagcggg taaaaccttc gcgaaaacgc tgcacgcgtc caccttcctg atctaccgcc
    45661 accaccagat gattgcgcga tgctgcgcgg atctggcgca ccagttcacg taactgggca
    45721 ggatcatgat agttacgcgt aaagagaatc agccctccca ccagcggatg cgccagtatt
    45781 tcacgctctt ccgcgtccag ttcgtaacct tcgacatcca acattactgg acccacactg
    45841 ctctccttat tgttttatta atagctgccg ccaggtgtca tcggccagcc tgataaattg
    45901 ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct cctttgagca tcagcagcca
    45961 gggaaaccat cgcctgacct gacgccataa ttgcgccgga taaatcttcg cgcgagtggc
    46021 atagtcattg accaattgcc ggtgctgttc agtattttcc acccacaccg ccgccagttc
    46081 cagcgcgata tcaccatctc cagcatactc ccagtcgatg agttttaacc ctgatgcgct
    46141 atgcactaaa tttccggcgt ggacatccat atgcaatgga cttaagcgta aaggtcgtgg
    46201 ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa cccacagtcc gccgcgccgg
    46261 atcgctttgc tgccagtaca gttccagtaa gggcaacagc gttattcgcc agccaaaacg
    46321 tggttgttga tgtagataat acagcaagcc tgccagttcg ttggtatccg gcaaatacgt
    46381 ttttaccgca ccgggcagat agtcgactac catccagtca cggagatata aatgcggctt
    46441 cggtgcaatg cttgcgggta gttgtgataa agcccgatac tggcgcaaga acgcggactg
    46501 cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc tggccctgat gttcaatgag
    46561 aaaactaccg ccactaagcc cactattaaa cgtcgtgacg ggatgaaact gcgggaaaaa
    46621 gcgcgacagc aattcgtcgc gcgtgatggg attattgctg cgaaacggca cctttacctg
    46681 accagataat ttcgcccgtc tgcaccagca tcagctgcat ttgtagggtc ggagcgttaa
    46741 cgttgccaga ggcgcaggag tacagcacgt aatgagcgcc gacattgcgg gcaatgccta
    46801 tggctttact acgggtgcct aaactgtcct gcggcgacaa acctaactgt tgcttagcca
    46861 tcgacagttg ctgggcggaa accagggtaa atttcccgtt attagccagc gcatttcgca
    46921 gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt acggttgtta acgctatcaa
    46981 ccagcaggac gctacccgca gtcaccccgt cagccccaag catcttactg accatcggct
    47041 gcattgcgcc attccagtca taatggcgaa tatgcggcgc aggcggtgcc gttcgatctt
    47101 cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac cgagggcact gtggggacag
    47161 gctgttgtgg ctcggctggt tgttccggcg ctggtttcac ttcttctacc ggtgcaggtt
    47221 cacgttgccc cacacacccg gcgagaaaca tcgccagcgc ggtaatcaag gcgtagcgac
    47281 tcatttttgt catcaagatt caccccttta caaatataga taaagtctga ctttgtgcgc
    47341 ccccagaaaa ttggcgctgc cgtacagcgt taccgccgaa tgtgcgggaa tggtaacgct
    47401 gcgtggcctt tccagaggat gcatctccag ccctctggcg tcataccagt aaaaacgata
    47461 atgaacggta acgggttctt gcctttcgtt ataaatcgtt gaggatgctg aaggttgaat
    47521 atcagacgtc gaaaggacgg gcttttctgc actgatgccc gcagccaata aagatgactc
    47581 catcaccagc gattgctcat cattcaccgg aatttccgga tgtgaacgac agcccaccag
    47641 cagtaacaac accagagaca ccagcccaaa gcatcctttt ctcatcgtta cagaccttta
    47701 tgcgccagca ttggtcccag cggacggcca cccaacaagt gcatatggat gtggtaaacc
    47761 tcttgtccgc catggcggtt ggtattcatg atcagacgat agccatcttc ggcaatacct
    47821 tcttgctcag caatttttgc cgctacggtg atcatgcgtc ccagcgcctg ctcatgctca
    47881 gctgacacgt cgttcacagt cggaatgaga atattcggaa tgatcagaat atgcgttggc
    47941 gcttgcggcg aaatatcgcg aaacgccgtt accagatcat cctggtagac gatatcggag
    48001 gggatctcac gacgaataat tttgctgaat atagtttctt ctgccacgac attttccttt
    48061 ttcaaatagc catgcgttgc gccatgctac gccgcgccac actgcgagta tagagtatgg
    48121 tcgagttaaa cctgctcttt caatcttaac tcacgatttc ttaagcaaaa aatcgttttg
    48181 ctgccgtata tatcgccatt attcccattt ctgcctgcga atgcgctgaa aagacattct
    48241 ctgtttggtt tacacgacaa atatgaatgc gtatatttct catttgcatt tgcagcaaaa
    48301 ttattcgcac actaaatata accgctcatc accctcctcc ggcatgagcc tgacgacaac
    48361 ataaaccaag agatttcaaa tgctttcaac acaatttaac agggataatc aatatcaagt
    48421 catcaccaaa ccgtcactac ttgccggttg catagcactg gcactattac cttctgccgc
    48481 ttttgctgca ccagccactg aagaaacggt gattgttgag ggttcagcca cagctccaga
    48541 tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg ggtaccaaaa tgcagatgac
    48601 tcaacatgat attcctcagt cggtcactat tgttagccag cagcggatgg aagatcagca
    48661 gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg atcagcaaaa gtcaggcgga
    48721 ttccgatcgt gctctttatt attcccgcgg attccagatc gataactata tggttgatgg
    48781 tatccccacc tattttgaat cgcgctggaa tctgggcgac gcactttctg atatggcact
    48841 gtttgaacgc gtagaagtag tgcgtggcgc gacaggactc atgaccggga cgggtaatcc
    48901 atctgcggca attaatatgg ttcgaaaaca cgcgacctgt cgtgaattta aaggcgatgt
    48961 ctcggcggaa tacggtagct ggaacaaaga acggtatgtg gcggatttac aaagcccact
    49021 caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc taccagaata acgactcatg
    49081 gctggaccgc tacaatagtg aaaagacctt cttctcgggc attgtcgatg ctgatttagg
    49141 cgatcttacg acgctttcag ccggttacga atatcagcgc attgatgtta atagcccaac
    49201 ctggggcggt ttaccgcgct ggaatactga tggcagcagc aacagttacg atcgcgcacg
    49261 cagtaccgca cctgactggg cgtacaacga taaagagatc aacaaggtct ttatgaccct
    49321 gaagcagcgg tttgctgata cctggcaagc gacactgaat gccacccact ctgaagtcga
    49381 atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac aaagcggatg gtatgctggt
    49441 tgggccatac agtaattatg gacctggctt tgatcatgtc ggcggcaccg gttggaacag
    49501 tggcaaacgt aaagttgatg cgctggattt gttcgctgac ggtagttatg aattgtttgg
    49561 tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa caaaacaatc gttcagttca
    49621 tgggccaaca tcttcccgga tgaaattggc agtttctaca actttaatgg caatttccca
    49681 caagccgact ggtcatcaca gagcctggcg caggacgata ccacacatat gaaatcgtta
    49741 tatgctgcca ctcgtgtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat
    49801 accaactggc gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttac
    49861 gctggtctgg tgtttgacat caatgacaac tggtcgacct acgccagcta tacctctatt
    49921 ttccagccgc aaaatgatcg tgacagttca ggcaaatatc tggctccaat caccggtaac
    49981 aactacgagc tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc
    50041 atcttccgta ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc
    50101 aacggcgaaa ccgcctataa agcggtggat gggacagtca gtaaaggggt ggaatttgaa
    50161 ctcaacggcg caattaccga caactggcag ctgacatttg gcgcaacgcg ctatcttgca
    50221 gaggataacg aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc
    50281 accagctatc ggttgcctgt catgccagag ttgacagtcg gcggtggtgt taactggcaa
    50341 aatcgcgtgt ataccgacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc
    50401 tacgcgctgg tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg
    50461 aacgtcaata acctgttcga caaaacctac gataccaaca tggaaggttc tatcgtctac
    50521 ggcgcaccgc gtaatttcag cattaccggc acgtatcaat tctgaataac acctgtaaaa
    50581 aaggcagcca tctggctgcc ttagtctccc caacgtctta cggattagtg gttacggatg
    50641 tactcatcca tctcggtttt caggttatcg gatttagtac cgaaaatagc ctgaacacca
    50701 gaaccagcaa ccactacgcc cgctgcgccc agtttcttca ggccagcctg atccacttta
    50761 gacacatcag caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt
    50821 tctttaccac caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgttgctttt
    50881 gcatcttcag tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg
    50941 cggaagatgg tgtagtaaac aatcgcataa ccgataccga cgatcggtaa cagccacagt
    51001 ttgctgctgt taccagacag aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg
    51061 tcacgcatcc ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg
    51121 atgtacagga tcggcgcaac gaacatgaag aagaactcga tcggctcggt gataccggtc
    51181 aggaacgagg tcagcgccgc ggagatcata ataccgccca ctttcgcgcg gttttctggt
    51241 ttagcagagt gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag
    51301 ccaccagaca gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg
    51361 aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag
    51421 atgtggtgca gaccaaacgg taccaggcaa cgttcgatga aaccgtaaat gccaaacgca
    51481 actaccgggt tctggtaagc agcccactga gagaaggtct ggattgcaga accaatcggc
    51541 ggccaaatga aggacagcac aacgccagta aagatggcag ccaggccaga aatgatcggc
    51601 acaaagcgtt taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg
    51661 ttaaacatgt acgctgcaat cgcaccggag ataatccctc cgagtacgcc agtatccgcc
    51721 aagtgtttag aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc
    51781 atggttttaa ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta
    51841 ttggtaaagc cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa
    51901 ccgcctgctt ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa
    51961 ccgacgccca gcagaatacc tgcgataggt agtacggata ccggcagcat cagcgattta
    52021 ccgacctttt gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat
    52081 gggtgctttt tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc
    52141 atctaagcgc cctttattta ttacacagag taaaataatt cagtgccaat ttgtttgacg
    52201 gctatcacgt ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc
    52261 acaatatttc aaacgcggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt
    52321 agccattaat tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg
    52381 tcgatgtgaa acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca
    52441 acacctttca acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct
    52501 tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taatcgatcc
    52561 agggggacat aacgcgcagc atcgcgcagt tgctccgcat tacggaaggt cacaatgccg
    52621 gaaaaggaga tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta
    52681 aaacagtgta gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta
    52741 tcggcacggg cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg
    52801 atatgatgga tgaaggactc ttgctgacgt actttagttt ccggtgtgta ataataatcc
    52861 agcccggttt cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct
    52921 acatcgtagg gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca
    52981 cgttcgccta ccagatcccg catatgtaaa taacccggta atgttgtggc gactgccaga
    53041 caaaatttca catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa
    53101 gattcataat ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc
    53161 ttaaagatga ggaaccggta acacaacgcc cggttgcagg taatgctcaa tacgcagtaa
    53221 aagatcggtg atgagaagct cgcggttgat gcctgtgaca gacattaact gttcacgaat
    53281 gtggcaaaca tcccccagta tagcctgcag gcacgaggga gaaagatggt ttgccagttc
    53341 ggcgaccagg cccggcacat caacattggt cacctgcgca gcgccatgat ggcgttttag
    53401 cgcatccatc agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt catgattaag
    53461 ggccgccagc agcgaatacc agtcgcccga tggcacgcta tatgccaacg cctgacacaa
    53521 tgtttcacga gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc
    53581 ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag
    53641 ccaagtcacg gcgtactgtt ctggaggcgg cgcaaggtaa tgtaaccgac aacgactacg
    53701 taatgttgcc agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg
    53761 tggctcttca agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc
    53821 ggttacccag acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac
    53881 ctcacgtacc gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta
    53941 atcgggatgc gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg
    54001 gccctgcggt tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc
    54061 catgcccggt aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc
    54121 taccagtttt tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc
    54181 acccagtggg tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca
    54241 tcaatggtat gaatactttt atcttgtgca gccagttcca gatagcgggc gcgggtgcga
    54301 ttaaagaaat cgaaagactc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt
    54361 tttaagccaa cttccggggt aacatcgaga tagagcgtta agtctgggcg aaaatccccg
    54421 agaacagcat cacgcagtgt tgccagcata tgttggtcaa taccacgtcc tccgccctga
    54481 tacgcctgag tggaaagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca
    54541 ggtttgatga ccgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct
    54601 ttatcggtaa tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt
    54661 tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc
    54721 tcgagcgtct caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca
    54781 atgacgatat acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga
    54841 cttgttatga ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa
    54901 atagagatac ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt
    54961 agctatcgca cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc
    55021 caggtctgca cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggttgg
    55081 gtcggtctgc aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc
    55141 gcgttcactg gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctgatt
    55201 tttatcttta taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac
    55261 cattttcttg tgaactcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcaa
    55321 ggtgtctggc cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc
    55381 tacggtggcg tacttatcgt cactcagcgt gtgcttgata tacggagcct cacgcaattg
    55441 cttgaggtag tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt
    55501 accgctttcc aacaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt
    55561 cccggcttta aagtgagaaa gatccggttc gatacgcagc agccattgaa aaacccgtgg
    55621 gcgattgatg atcttatcag cataaagctg ttcaccgagc gccagacgtc cggtccctgg
    55681 cttcagggta aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac
    55741 cttccagacg cccacaccag cggcgatacc cagtaccacc agcaataaca agataatcaa
    55801 taacactttt ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac
    55861 gttgctgacg aaaaggtgac atcgccacag gcacgtacgg gcatcactgg cattaacgca
    55921 ttacaaataa ccatctcatc tgcctgcaac gcctcctcca gcgaggcttg cacttcgaca
    55981 agctgataag aggattgtgc cagtaaacgg atacagaatt gtcgcataat gccgttaaca
    56041 cctgcctgat ccagtcgcgg cgtataaact acattgccct tccgccagaa caaattagcc
    56101 gcacagcatt ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc
    56161 tgctcaagat gagagcgaat caatacttgc tcaagccgat taagatgttt aatacctgca
    56221 agatgaggat tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc
    56281 aaacggtcgt aatgtgcagg ataagccgta acggagagaa tccgcgttgc tggtccgctg
    56341 ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagcacacca
    56401 ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag
    56461 gaaatcatca accgctgaca agcatcctgt agtcgcagga tatgcgccga taacaaactg
    56521 actttaccgt cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga
    56581 tcgcttactg ccagcgattc ctgcttataa ccgttaatta agaacatacc ggctccttat
    56641 cagagaattg ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga
    56701 aaaaaggccc gcaagcggac cttttatatg ggtggaaaat gacaacttag atctttttaa
    56761 agatcaaaga accattagtg ccaccgaagc cgaaggagtt acacagcgtg tattccattc
    56821 cgctgacctg acgtgcttca tgcggtacga aatccagatc gcaaccttca tccgggttat
    56881 ccaggttgat ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt
    56941 ctactgcacc cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca
    57001 cacggcttgc agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag
    57061 ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat
    57121 cacgcagagc atttgccatt gccagagctg cgcctgcgcc attttctggc ggtgacgtca
    57181 tatgataagc atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc
    57241 gttttttcgc gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga
    57301 agccatcacg ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag
    57361 ataatgcacg tgccgcgcca aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc
    57421 cacctgcaac catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt
    57481 tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca
    57541 tgatagtcag atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga
    57601 tcttacgtgg accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc
    57661 caatcccgga gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc
    57721 cagaatcctg catggcctga acgccagcga caattccata ttgaatgaag gcatccatct
    57781 tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa
    57841 atttcgttgc ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg
    57901 caagcagagc tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc
    57961 cggtcacaac tacacgacgc ttagacacgt ttgtcctcca aggagggaaa aatgattcta
    58021 gtgggacaaa aagataaaac tcaggcggtc gaacgaccgc ctggagatgt tcacttacgc
    58081 ctggtggccg ttgatgtaat caatggcagc ctgaacggtg gtgattttct cagcttcttc
    58141 gtccggaatc tcagtatcaa actcttcttc cagagccatt accagctcaa cggtgtcaag
    58201 agaatccgcg cccaggtctt caacgaaaga agcattgttg gtaacttctt cctgcttaac
    58261 gcccagctgt tcgccgataa ttttcttaac gcgttcttcg atagtgctca tactcttaaa
    58321 tttcctatca aaactcgctt tcgcgatggt tttcgtagtg tataaaatgt tgaaaaattt
    58381 gcaactaaat cccggcaggt cttaccacga ttttacgtta ttttgaggcc ttacccccca
    58441 ataacgcaaa tcattttgca ctaattatta ctcataacca cgcagactgc gcgcaacatg
    58501 agcattttgt gcaaatcgcg gtcagaccat gtacatcccg ccgttcacat gcaaagtttc
    58561 acccgtgatg taagctgctt cgtcggatgc caggaatgca accgcgttgg cgatttcctg
    58621 tgcgccgccg aggcgacccg caggaacctg cgccaggata cccgcacgct ggtcatcgct
    58681 cagcgcacgt gtcatgtccg tttcaataaa gcccggagca acaacgttta cagtaatacc
    58741 gcgtgacgca acttcgcgcg ccagtgattt actgaagccg atcaagcccg ctttcgccgc
    58801 agcgtagttg gcctgaccgc catttcccat ggtaccaacc acagaaccga tagtgataat
    58861 acgaccatga cgctttttca tcatagcgcg cattaccgct tttgacagac ggaaaacaga
    58921 tgaaaggtta gtttcgataa tatcgttcca ctcttcatct ttcattcgca ttaacaggtt
    58981 atcacgagtg ataccggcat tattgaccag gatatccact tcaccaaatt ctgcgcgaat
    59041 tttttccaga acagattcga tagatgccgg gtcggtcaca ttcaacatca gacctttgcc
    59101 gttggcacct aaataatcac tgatcgcctg agcgccattt tcactggtcg cagtgccaat
    59161 aactttcgcg ccacgggctg cgagcgtttc agcaattgcg cggccaattc cgcggcttgc
    59221 accggttacc agtgcgattt ttccttcaaa attcatgatt ttcctctttt aaagctcgag
    59281 cgccgctgcc atcgctgaag gttcgttcag cgccgaggcg gtcagggtgt cgacaatgcg
    59341 tttcgtcagg ccagtaagca ccttgcccgg gccgacttca tagagatgtt ctacgccttg
    59401 cgctgccatg tactcaacag acttcgtcca ctgaaccggg ttatacaact gacgtaccag
    59461 tgcgtcacgg atggcatcac cattggtttc gcatttcaca tcaacgttat tcacaacaga
    59521 aactgttggt gcgttaaagg tgattttcgc taattctact gccagtttgt cggctgctgg
    59581 tttcatcagc gcacagtgag acggtacgct cactggtaac ggcagcgcgc gttttgcgcc
    59641 agcagcttta caggcagcgc cagcacgctc aaccgcttct ttatgaccgg caataaccac
    59701 ctgtcccgga gagttaaagt ttaccggaga aacgacctga ccttctgcag cttcttcaca
    59761 cgctttcgca ataggcgcat catccagacc gatgattgcc gccatagcgc ccgtgccttc
    59821 cggtacggct tcttgcatga acttaccgcg catctcaacc agacgcaccg catcagcgaa
    59881 atcaatcacg ccagcgcaaa ccagcgcgga gtattccccc aggctgtgac cggccatcat
    59941 tgccggtgct ttaccgccct gctgctgcca tacgcgatac agcgcaacag atgcagtcaa
    60001 cagcgctggc tgggtttgcc aggttttatt cagttcttca gctgacccct gctgggtcag
    60061 cgcccacagg tcgtagccca gcgccgcaga agcttcagca aacgtttctt cgacaattgg
    60121 atagctcgcc gccatatcag ccagcattcc aacggtttga gaaccctgtc cagggaacac
    60181 aaatgcaaat tgcgtcatgt tttaatcctt atcctagaaa cgaaccagcg cagagcccca
    60241 agtgaatcca ccgccaaagg cttcaagcag aaccaactgc cccggcttaa tgcgcccgtc
    60301 gcgtacagct tcatccagcg cgcacgggac agaggccgca gaggtattac cgtggcgatc
    60361 cagcgtcacc acgacattgt ccatcgacat accgagtttt ttcgccgttg cactgataat
    60421 acgcaggtta gcctgatgcg gaaccagcca gtccagttga gaacggtcaa gattattcgc
    60481 cgccagcgtc tcatcaacga tgtgcgccag ttccgttacc gcaaccttga agacttcgtt
    60541 gcccgccatc gtcagatgaa ttgaattctc tggattcacg cggtcggcgt ttggcagcgt
    60601 cagcaattca ccataactac cgtcggcatg cagatgggtg gaaatgattc ccggctcttc
    60661 agaggcagcc agcaccgcag cgcccgcgcc atcgccaaaa ataataatag tcccacgatc
    60721 tgttggatcg caggtgcgcg ccagtacatc ggaaccgacg accagagcat acttcaccgc
    60781 cccagatttc acgtattgat cggctacgct taatgcatag gtgaaacctg cacaggctgc
    60841 tgcaacgtca aatgccgggc aacctttaat gtccaacatg ctttgaatct gacaagctgc
    60901 gctcgggaaa gcgtgcgtag cagaagtcgt tgccacaacg atcaggccaa tctggtcttt
    60961 ctcaatgccc gccatctcaa ttgcgcgtgt cgccgcttca aagcccatgg ttgaaacggt
    61021 ttcgtttggc gcggcaatgt ggcgttcgcg gataccggta cgagtgacaa tccactcgtc
    61081 agaggtgtcc accatttttt ccaaatcggc gtttgtccgc acttgttcgg gcagatagct
    61141 gccagtacca ataatcttcg tatacatgta cgctcagtca cttttcggtt atataccgtc
    61201 acttgcaaac tgcgagttcg ctggcagcgt cctgctaccg cagagttccg cttttgccac
    61261 cgtccagcag ctcaaaacca gctgggtata cagattccag gcgagcggca attcgctgag
    61321 gaacttgtcg ctgcaccgcc tgcactgcct gttcaatcgc gaccgcaaaa gctcgctgat
    61381 tggctgcacc atgacttttt atcaccgtgc cgcgcaatcc taacagacag gcgccgttat
    61441 actggtcggg gttgaggtga ctgaatcgcc tcgtcaggct cttttgtagc caacgcttta
    61501 ataacagtag ccaccacgac cgttttttcc cttcaccctg agatttcagc agagaaagga
    61561 acatcctgac aacaccttcc atcgtcttta acgtgacatt tcctgtaaag ccgtcacaaa
    61621 ccagcacatc tgtcttgcca gttaacaact cattggcttc aagatagccg atataattga
    61681 tagaagggat tgttttaagc accgctgagg catcccgaat actgtcaaga ccctttactt
    61741 cttcttcacc aatattgagc aacgccacgc gaggattggg aatttccacc acctcttcag
    61801 ccagaactga gcccataatg gcaaattgca ccaacattgt gctgtcacaa tcgacgttgg
    61861 cccctaaatc gaggaccacc gttttgccct tttgctgatg tggtaatacc gtcaccagcg
    61921 ccggacgctc aatcccctcc aggggcttga gtaataattt tgccagcccc atcagtgccc
    61981 cggtattacc ggcactgaca caggcttgcg ctcgaccttc tttcaccagc tccagggcca
    62041 tacgcattga actcccacgg ctggcgcgga tagcttgcga aggccgggca tcactggcga
    62101 taactgactg cgcaggaata atctgcagac gcgaacgttg ttcaaagtca gctttagcaa
    62161 gtaatggcgt gatggcgtcg ggattgccga ctaaaagaag agtgagttgc gaattagaat
    62221 tcagtgcctg caatgctgca ggcactgtca cggaagggcc aaaatcccct cccatgacat
    62281 ctaacgccag ggttagacgt gtcaaggtat cgtcgccgcc cggtttggtc tttccccagt
    62341 tgcctgggga aaatcctcac taagcttcat cacgcagacg cgtgattact tagcgatgac
    62401 cttgcggccg cggtagtaac cgtcggcagt gatgtggtga cgcaggtgtt tttcaccaga
    62461 agttttgtct acagacaggc tggtgactgc ggtcagcgcg tcatgggaac gacgcatgcc
    62521 acgtttggaa cgggttggtt tattctgttg tacggccatg gaccttactc ctcaattacg
    62581 gtttatcccc gatccaacgg ggagcaccaa ttacttacgc tttaagctgg ctaatacggc
    62641 aaatgggttt ggcttttgca cttcttcagg cagttcacca aagaccatgt ccgcttcgga
    62701 cacttcacag tgttcagaat catgcaccgg aactaccggc aaggcgagga tgatttcatc
    62761 ttcaaccatt gcaagcagat cgatttcacc gaattcgtta acctcaatcg gttcatacgc
    62821 ttccggcagt gcttcagcct gttcgtctga acgcacagga ctaaaacaat acgttgtgta
    62881 gacctgatga gtaaacggct tcccgcaacg ctgacactcg agcgttaccg tcaccttcgc
    62941 atcgccgttt aacactgcga gacgttggtt atcgatagcg aacgacatgg agcattccac
    63001 atcactgtcc acactgacta cggattcggc gacgcgctca acctgatcag gggtatagat
    63061 accctggtaa tcaaggcgtt tttgagccgt acgaaccgga tcgagagtca ggggtaattt
    63121 taccttttgc atagggcgcg catattaact ttgtaacgtc atagagtcaa agaaaaaggc
    63181 agcctgtggt tgccttttgc caataattcg cacacattgc gggttactgc tttattttcg
    63241 ttcagtgctg ataacgaaca tcaacctgca aaacgaagcg catagtttaa aatggctatc
    63301 atcaatacgc tatatctgat ggaaaaaaat atgcctaaac ttattttagc ctccacctcg
    63361 ccatggcgtc gcgctcttct ggaaaaactg caaatctctt ttgaatgtgc agcacctgag
    63421 gtcgacgaaa ccccacgcag cgacgaatca ccgcgacagt tggtgcttcg actggcacaa
    63481 gaaaaatcgc aatctctggc gtcacgttat ccggatcatt taattattgg ttcagaccag
    63541 gtatgtgttc ttgatggcga aatcaccggt aaaccgttaa cggaagaaaa tgcccgtctg
    63601 caattacgca aagccagcgg caatatcgtc accttctata ctggactggc gttgtttaat
    63661 tcggcgaatg ggcatctaca aacagaagtg gagccttttg acgtccattt ccgtcaccta
    63721 agcgaggcgg agattgataa ttacgtgcgt aaggagcatc ccctgcactg cgcgggtagc
    63781 tttaagagtg aaggatttgg cattacgctg tttgagcgtt tagagggacg tgaccctaac
    63841 acgctggttg gtttaccgct aatcgcgctg tgtcagatgt tacgtcggga agggaaaaac
    63901 ccgctgatgg gatagttgtt tgcgccggat gcggttacgt cgcatccggc aatcggtgca
    63961 ggcggcctga tgcggcgtaa acgccttatc aggccaaccg cccattatca ggcttatatg
    64021 cttgtattag cgcgcgttac gcagcttttg caaacaacgc ttcaaacctt catccatcgg
    64081 cgcttcgata cgcatcacct caccggtccc cggatgggta aacttcaacg ctgcggcgtg
    64141 caggaacaga cgatttaatc ccgtgcctgc ttcagtgagc tgtctgtcaa attcacggtc
    64201 accgtagcga tcgtcaaagg caatcggatg acccgcatac tgtgtatgca cacggatctg
    64261 atgagtacgt ccggttaccg ggctacaacg caccagggtg gcaaatgcat agcgttcttc
    64321 cactttaaag cgtgtttccg acggtttgcc ttcctgactg acacgcacga tacgttcgcc
    64381 gctttgcaga atatttttca ataacggcgc ttgaacgctc ttcacatgcg actgccactg
    64441 accgcgcacc agcgccaggt aatctttttg cattcctttt tcacgtaatt gctcatgcag
    64501 agaacgcaac gccgagcgtt ttttcgctac cagcaacaca cctgaagtgt cccggtcgag
    64561 acgatgaacc agttcaagga accgcgcttc cgggcgcaac gcccgcaaac cttcgataac
    64621 gccgaagctt aaaccactgc cgccatgtac cgccgtaccg gaaggtttat tcagcaccag
    64681 gatatgatca tcttcatata agatgacgtc cgccagcgcc gccacctttt gcagatgtgg
    64741 cgaaaccgcc tcttcttccc gctcagcaac gcgaaccggt gggatacgca cctcatcacc
    64801 cgcttcgagt ttatattcag gcttaatacg ttttttgttc acccgcactt cacctttacg
    64861 caaaatacgg taaatcatac tttttggtac gcctttcaat tgggtacgca aaaagttatc
    64921 gatacgttgc cccgcttcgt cagcggtgat agcaacaatt tttacggatg gagtctctgt
    64981 tttcatggtg ggcgattcta attagccaac aggattcgcg ccactcattt ttctatgctt
    65041 atttttagtt ttgcacctta ttacttcact acgtgatcac tttattgatg gttattaaac
    65101 caatcaccag caagaagtga aaaaactgtg agtaagcggg tgataaatgg taaaagtcat
    65161 cttgctataa caaggcttgc agtggaataa tgaggccgtt tccgtgtcca tccttgttaa
    65221 aacaagaaat tttacggaat aacccatttt gcccgaccga tcatccacgc agcaatggcg
    65281 taagacgtat tgatctttca ggcagttagc gggctgcggg ttgcagtcct taccggtaga
    65341 tggaaatatt tctggagagt aatacccagt ctgtttcttt gataattgcg ctgtttttcc
    65401 gcatgaaaaa cgggcaaccg acactctgcg cctctttgag ctgacgataa ccgtgaggtt
    65461 ggcgacgcga ctagacacga ggccatcggt tcacacccgg aaaggcgtta ctttgcccgc
    65521 agcttagtcg tcaatgtaag aataatgagt aagttacgat gaaaagaatg ttaatcaacg
    65581 caactcagca ggaagagttg cgcgttgccc ttgtagatgg gcagcgtctg tatgacctgg
    65641 atatcgaaag tccagggcac gagcagaaaa aggcaaacat ctacaaaggt aaaatcaccc
    65701 gcattgaacc gagtctggaa gctgcttttg ttgattacgg cgctgaacgt cacggtttcc
    65761 tcccactaaa agaaattgcc cgcgaatatt tccctgctaa ctacagtgct catggtcgtc
    65821 ccaacattaa agatgtgttg cgtgaaggtc aggaagtcat tgttcagatc gataaagaag
    65881 agcgcggcaa caaaggcgcg gcattaacca cctttatcag tctggcgggt agctatctgg
    65941 ttctgatgcc aaataacccg cgcgcaggtg gcatttctcg ccgtatcgaa ggcgatgacc
    66001 gtaccgaatt aaaagaagca ctggcaagcc ttgaactgcc ggaaggcatg gggcttatcg
    66061 tgcgcaccgc tggcgtaggc aaatctgctg aggcgctgca atgggattta agcttccgcc
    66121 tgaaacactg ggaagccatc aaaaaagccg ctgaaagccg cccggctccg ttcctgattc
    66181 atcaggagag caacgtaatc gttcgcgcct tccgcgatta tttacgtcag gatatcggcg
    66241 aaatccttat cgataacccg aaagtcctcg aactggcacg tcagcatatc gctgcattag
    66301 gtcgcccgga tttcagcagc aaaatcaaac tgtacaccgg cgagatcccg ctgttcagcc
    66361 actaccagat cgagtcgcag atcgagtccg ccttccagcg tgaagtccgt ctgccatccg
    66421 gtggttccat tgttatcgac agcaccgaag cgttaacagc catcgacatc aactccgcac
    66481 gcgcgacccg cggcggcgat atcgaagaaa ctgcgtttaa caccaacctc gaagccgccg
    66541 atgagattgc tcgtcagctg cgcctgcgtg acctcggcgg cctgattgtt atcgacttca
    66601 tcgacatgac gccagtacgc caccagcgtg cggtagaaaa ccgtctgcgt gaagcggtgc
    66661 gtcaggaccg tgcgcgtatt caaatcagcc atatttctcg ctttggcctg ctggaaatgt
    66721 cccgccagcg cctgagccca tcactgggtg aatccagcca tcacgtctgc ccgcgctgct
    66781 ccggtaccgg taccgtgcgt gacaacgaat cgctgtcgct ctctattctg cgtttgatcg
    66841 aagaagaagc gctgaaagag aacacccagg aagttcacgc cattgttcct gtgccaatcg
    66901 cttcttatct gctgaatgaa aaacgttctg cggtgaatgc gattgaaacg cgtcaggacg
    66961 gtgttcgctg cgtgattgtg ccaaacgatc agatggaaac cccgcactac cacgtgttgc
    67021 gcgtgcgcaa aggggaagaa acgccgacct taagctacat gctgccgaag ctgcatgaag
    67081 aagcgatggc gctgccgtct gaagaagagt tcgctgaacg taagcgtcct gaacaacctg
    67141 cactggcaac ctttgccatg ccggatgtac cgccagcgcc aaccccagct gaacctgccg
    67201 cgactgtcgt agccccagca ccgaaagctg caacggcaac accagcagct cctgcacaac
    67261 ctgggctgtt aagccgcttc ttcggcgcac tgaaagcgct gttcagcggt ggtgaagaag
    67321 ccaaaccgac cgagcaacca acaccgaaag cagaagcgaa accggaacgt caacaggatc
    67381 gtcgcaaacc tcgtcagagc aaccgccgtg accgtaatga gcgccgcgac acccgtagtg
    67441 aacgtactga aggcagcgat aatcgcgaag aaaaccgtcg taatcgtcgc caggcacagc
    67501 agcaaactgc cgagacgcgt gagagccgtc agcaggctga ggtaacggaa aaagcacgta
    67561 ccaccgacga gcagcaagtg ccgcgtcgtg aacgtagccg ccgccgtaac gatgataaac
    67621 gtcaggcgca acaagaagcg aaggcgctga atgttgaaga gcaatctgtt caggaaaccg
    67681 aacaggaaga acgtgtacgt ccggttcagc cgcgtcgtaa acagcgtcag ctcaatcaga
    67741 aagtgcgtta cgagcaaagc gtagccgaag aagcggtagt cgcaccggtg gttgaagaaa
    67801 ctgccgctgc cgaaccaatt gttcaggaag ctccagctcc acgcacagaa ctggtgaaag
    67861 tcccgctgcc agtcgtagcg caaactgcac cagaacagca agaagagaac aatgctgata
    67921 accgtgacaa cggtggcatg ccgcgtcgtt ctcgccgctc gcctcgtcac ctgcgcgtga
    67981 gtggtcagcg tcgtcgtcgc tatcgtgacg agcgttatcc aacccagtcg ccaatgccgt
    68041 tgaccgtagc gtgcgcgtct ccggaactgg cctctggcaa agtctggatc cgctatccaa
    68101 ttgtacgtcc gcaagatgta caagttgaag agcagcgcga acaggaagaa gtacaagtgc
    68161 agccgatggt gactgaggtc cctgtcgccg ccgctgtcga acctgttgtt agcgcgccag
    68221 ttgttgaaga aatggctgaa gtcgtagaag cccccgtacc ggttgccgaa ccgcaaccgg
    68281 aagtggttga aacgacgcat cctgaagtga ttgccgccgc ggtaactgaa cagccgcagg
    68341 tgattaccga gtctgatgtt gccgtagccc aggaagtcgc agaacacgca gaaccgatgg
    68401 ttgaaccgca ggaagagacg gcagatgcag atattgaaga agttgccgaa actgctgagg
    68461 ttgtggttgc tgaacctgaa gttgttgctc aacctgccgc gccagtcgtc gctgaagtcg
    68521 cagcagaagt tgaaacggtg gccgcggttg aaccagagat caccgttgag cataaccacg
    68581 ctaccgcgcc aatgacgcgc gctccggcac cggaatatgt tccggaggca ccgcgtcaca
    68641 gtgactggca gcgccctact tttgccttcg aaggtaaagg tgccgcaggt ggtcatacgg
    68701 caacgcatca tgcctctgcc gctcctgcgc gtccgcaacc tgttgagtaa taattagctc
    68761 aaagtaatca agccctggta actgccaggg cttttttatt tcatgtttga atcaacatat
    68821 taaaattcat caaatggcat ccttgctaac caacaatgca aaataggcaa aaaaaatgcc
    68881 cgccccggcg atcccagagc gggcagacaa aacataccca gtttcatgat atgttttaaa
    68941 gcgagcttat ttgttgagct ggaagagcga caatccctgc atatcggtaa atgctttata
    69001 cgatgcctgc aatgccgttt gctgcatgat gtaagatgaa atagttgcat tccagtccac
    69061 atcaaccaga tcgctcatct gctgcgtttg ccctaaagcg cgatcgctac ctaaggaatc
    69121 cagcgactcc agtttgttca gctgcgtgcc taattccgcg cgaacggtaa ggacattatt
    69181 cagcgagttt ttcagtccac ggttggtttt atccaatgcc gcagcggcga tttctttatc
    69241 cgcttcgcta tccgcgaccg gcgttttcag ggctgcgatg gcgctatcca gcatggcaaa
    69301 aagattggtt tcagaagcgc taccgtctgg ttccgctacc gcgttactgg taatactgtc
    69361 gaaaattttg tcgcccgtat gtcctatcac catcgaacgc gaagcatcga cctgttgctt
    69421 aatactttct gttccgccaa cataatcacc gttagcctca ctaaacggcg cagtctctgt
    69481 tttatatgta gcgcccatta ccgtcagtgg tgttcgccag attcagcaac tggtcacgaa
    69541 gcccctgaat atccgtagcc agcgaggccc ggtcatcgtc actcaaggtg ccattgctgg
    69601 cgtagacaat tttttcctga gcattctgga tagcagtggt gacctggcta agtacactct
    69661 cttccagtga cactttttga gtggcgaaag tacgtgccag cgtgtactgg ctgttttgcg
    69721 cctgtgcctg ggagagaaca acagcttgtg atgcagcaat gggatcgtct gaagggttaa
    69781 cgactcgctt acccgtcgac atctgctcgc cgtacttcat ccattctgcc tgagaattgg
    69841 tgatgccgcg catgttttgc tggtacatca tctgggtact gaaacgcatt ttatctcccc
    69901 ttagcgaatg ttaatcagcg catcaaaaat cgcgtttgcc gtctgcagaa cctgcgcatt
    69961 cgccaggtaa tactgctgaa aacgttgcag atttccgtac tcctcatcga gattgacacc
    70021 ggaaatcgac tgctgctgat tggaaagctg cgtcaccaca ttaccttgcg tggtgctact
    70081 ggttttcaac gtcgcggttt tattaccgat atcactcact aacgaggcat aagcgtcgtt
    70141 aaaggatttc gcaccgccca ccgttttact gttgctttgc agatccagca gggcctgacc
    70201 gttgcggttg tcgctatcac ccgcatcttc ttcgctcgcc atcgcgattt tcgcttcgtc
    70261 ggtgattaat acatccatgt tgacgatggc gtcacttact ggtttcagcg tgaagctgtc
    70321 gttaacggca ggcgttcctg taaacgtcaa ctccagacca tcaaatgcca ctttaccgtt
    70381 ggcatctggt gtcgccgtaa aagtggtatt gctggcaagg cgggtgacct gccactgatt
    70441 attatcgaac gagattttgt aatctgtcgc cagtaccacg gaggcatcag ttaccgtggc
    70501 accgatcgca acgtcacctt tgtttttcgt gttttgcaga accgcgggct taccgatagt
    70561 aaagaaatct tcaccagcat cgccgttagc atcaaatccg gctttgtgtt ggctgttgaa
    70621 agcctcggca aatgccagcg ccagttgtcc aagcgtatta cgcgtctggt ccagatcctg
    70681 agaacggaat gtcagaatgc cgcccagcga cccggtattc agtaatttct ccgggatctc
    70741 aatattgcct gccgtcccat caacgtaagc gacagtcgta cgagaagggt cggcgctgga
    70801 aggaactgcc gccagttgcc gcgccgtact tccctgaacc agtgagtaac cattggcatc
    70861 gtgatgttat aagtaccgcc atcctgaacg ctgacttcta cgccaacaat ctggtttaat
    70921 tcgctcacca gttgatcgcg ttgatccagc agattgttag gtgacgcccc tgcccccacg
    70981 cctgtcaggc gcgagatttg atcgttcagg ctggcaattt gtttagcgta attgttgatc
    71041 tgatcaacgc tggcacctat cgcgatattg acctgtttgt cctggtcgcg cagatattga
    71101 tcggtggttt taaactgatt caccaaccct tctgatttcc caatcagcgc ctggcgcgcc
    71161 gccgggtctt ccgcgttact caccagcgtt tgcaggctgg tgaagaaatc ctgcatctgt
    71221 gttgccagcg aagaggtact ggtggagagc atattgtcga ttttcgacat ctgctcatag
    71281 cgggcggtca ggccgctact ttgcgtctgc gcagcacgta actggttggt aataaacgta
    71341 tcatactcac gctgcacacc agaaacgtag acgccattgc caacccagcc gccagcgccc
    71401 aacgtgctat tggcctgcgc cataatagtg gtttggcggg tatatccggc aacgttatag
    71461 ctggagatat tattacttgc cgtatttaac gccgcctggg ccgcgttcag tccgctcatg
    71521 gcgttattga tcaagctgga catggaggtt ccttataagc cttcaattac agagtattat
    71581 cggcagcgac ccgccggact tgagttattc agaacagatt atcaatgttc atgctgtagg
    71641 ttttgctcac cttgtcgctt atcgatttca tctgctggat catgttggtg agtttacggg
    71701 catagtgagg atcggtagca tagcccgcgt cctgtaggac ctgcgccccc tgttccgcac
    71761 tcgcggcggt cgtcacggcg gcgtagcgcg ggttacgtgt taacagccca acgtaatccg
    71821 acaatgcttc cagatacgag ctgtagaccc gaaacttcgc ttttactttc ttcgcttcgc
    71881 cgttttcata ttcagtcgtg gtgatttcag tgacttgccc tttccagttg ccagaggctt
    71941 tgacaccaaa caggttatag cttggctcgc cgttttcgcg gcggatttgc cgttgccccc
    72001 aaccagattc aagcgcagcc tgagcgagga tcaaatgatg tggcacaccg ctttgctggc
    72061 ttgccagttg ggcgggcagc gagagttgcg cgaggaatgc tctactgtca cccggcagcg
    72121 aatcatcgta gttacgtggt acggcctttt gcaccagctg cgaaagcgtc tggttttgat
    72181 aacgcaccac ggtttcgagc gggaatttca tcggtgctgc tggcgtggac tcctctggca
    72241 atggttgttc tggcgtcatc tgtttaacca tcatctctgc aagccccaga cctttgcccg
    72301 ccgtcatctg ttgggcaatt tgctggtcat acatactggt atacagacga gtgtgctcgc
    72361 tgctgaacag gccatctttt ggtaaagcgt cgcgcatgct tttcaacatc atctgcacga
    72421 acatcccttc cacctgacgg gccaccggac ggatatttgc cgccggatct tcgctcgctt
    72481 tcgcctttag ttcgttgagt gattgcgcat cccaggccgc acttgccagt agtttgctgt
    72541 cgctgatcat cagatgattt ccagttttgc ccgcagacat cccgcacttt gcattgattg
    72601 caaaatagac atcagatcca tcggcgtagc gcccagcgca ttgagcgcac gcaccacgtt
    72661 attgaggctg gcgctggaac gtacgctttg cagcgaaccg ccgctctggc gtaaatcgat
    72721 ctgcgtttgt ggagtaacaa cagtctgtcc accaccaaac ggtgtatctg gctggctgac
    72781 attggcctga cgattaactg ttactgagag atttccctgc gctaccgcgc agctgtcgag
    72841 ggtcacttcg cgattcatca ccaccgaacc ggtgcgcgag ttaatcacta ctttagcgtc
    72901 ctgcggggtg acattaacct gcatattctg gatatcggca aggaaacgga cctgggaact
    72961 gttgccactt ggtacgcgca cctgaatagt ccgcgcatct aacgcggtgg cgctgccata
    73021 tccacgcacg cggttgatgg tgtcagcgat ttgctgcgcc atgctgaaat cttcgtcgtt
    73081 aagttgcaaa ttaagggtat tcccgacgcc aaactggctg ggcaattcac gttcaataac
    73141 cgcaccattg gtgatccgtc caccgttcag ttggttaacc tgaacactgc taccgcctgc
    73201 ggaggctcct gcgccgccaa ccagaatatt gccctgcgcc agcgcataca cctgactgtc
    73261 aacgccctta agcggagtca tcaacaacgt acctccacgc aagcttttgg cattacccat
    73321 ggaagaaacc accacatcga tggtttgccc ctgacgtcca aacggaggaa gtgacgctgt
    73381 caccattacc gcagcgacgt tttttagctg catattggtg cccgtcggaa cggtaattcc
    73441 cagctgtgag agcatgttat taagcgtttg tgtggtaaac ggcgtctggg ttgtctggtc
    73501 accagtgcca tccagcccca ctaccaagcc atagccaatc agtgagtttt gccttacccc
    73561 ctgaacactg gtgagatcgc gaatacgctc agcctgagcc gccgtcgtaa ccagtagaag
    73621 aattaatgca gagagaaatt taatcaccac agcctcactt acattggcga caggttaagg
    73681 aagaaacgct gcaaccagcc catattttgc gcttcgttaa tgtagccatt gcctacgtat
    73741 tcaatacgcg catccgccac ctgagtagac ggtacggtat tgctgccgct gatagtgcgt
    73801 ggattaacca cgcccgagaa gcgaataaat tcggtaccct gattaatggc aatctgtttt
    73861 tcacccacca catgcaggtt gccgttgacc agtacctggt caaccgtcac cgtcaacgtg
    73921 ccgctaaagg tattgctggc attggccccg ccctttccgt tgaacgtgtt accaccggag
    73981 gcttcgacat cggcacgagc gttaccaaac agcccctgca aatagcgcgg cacagtatca
    74041 aagccaaaat tagttttacc gtcacggctg gcattcgcag aggagctttt gctggcgctg
    74101 acgttctcct gcaacacgat ggtcagcgta tcgccaatat tgcgtggtcg acgatcttca
    74161 aacagcggtt gatagccata gttaatcggc tgagcagact ggaaaataga accgttggcg
    74221 acgggcgtcg gaccgggaac cggttgtgca ctggtcgccc cctgcaccag cggcgtggag
    74281 ggtatccagg cgcagccggt tagtgaaagc accaacaagc tggaaatggc ataagtatgc
    74341 gcagcgtttt tttgcatggc tatcatcttc aaaaatcagt aaaccggtga acctgccacc
    74401 ggttatgcct tagagttgcg tcagtttttg cagcatctga tcggtggtgg acaccgcttt
    74461 actgttgatt tcgtaagcgc gttgcacctg aatcatattg accagttctt ccgccacgtt
    74521 gacgttagac gtttcaacat acccttgata cagcagtccc gcgccgttca ggcccggcgt
    74581 gctttcgttc ggtgcaccgg aggattgcgt ttcggtgtag aggttttcgc caatgctctc
    74641 cagtccggta tcattcataa aggtggtgag attgagctgc ccaacctgaa ccggagctgc
    74701 ctggccttgt tgggttacgc tgaccacgcc atcacgaccg atggtgatac ttaacgcatt
    74761 cgccggaatg gtgatcgctg gctgcacctg aaaaccacca gccgtcacca gctgcccgtt
    74821 ctgatccacc tggaaagagc cgtcacggat ataggctgat gagccatctg gcaacattac
    74881 ctggaaaaag ccctgccctt taatcgcgac atctttgctg ttattggtct gcgacagatt
    74941 gccctggctg tgtaagcgtt cagtggcgac cgggcgtacc cccgtgccga tttgtaatcc
    75001 ggagggtaag gtggtttgtt cggaagactg tgcccccggc tggcgaatgg tttgataaag
    75061 cagatcttca aacaccgcgc gctgacgctt aaaaccgtta gtactgacgt ttgccaggtt
    75121 gttggcaatg acgtccatat tggtttgctg ggcgtcaagg cccgttttgg cgatccataa
    75181 tgaactgatc atataatgtc ctgtagtgga ttgctgacaa gcagagcaga agcacgcgac
    75241 agtgtgaatt tcccctcacc ctaaccctct ccccaaaggg gcgagggaat agtccgcgcg
    75301 cttttattgc aatgtcatca atcgttcaat taactcatcg acagcagttg gttggcacgg
    75361 cctgcgttat catcgacgct gctgatcacc ttcatctgca tttcaaaacg ccgcgcgctg
    75421 gcaatcatgt cgctcattgc cgcaacggca ttgacgttac tgccttccag aacccccgac
    75481 atcacacgca aggttggatc tgcctgcagt accggccctc gcgtggcctg ggtttctgcg
    75541 cttaaacgaa aaatgccgtc gtcaccgcgc tgcacttcgc tgcccgtggc tttcaccagt
    75601 ttcagacgcc ctactggcgc aactgtattt gccggatcgc ccggattgag cgccgagatt
    75661 gtgccatcgg cagcaatcgt gatttccgcc ccttccggca cagcaattgg ccccgcctcg
    75721 cctatcaccg gatgcccctg aattgtcagt tgcccggtgg gatcaacctg aatgctgcca
    75781 ttacgcgtat acccttcgct gccgtcagcg gtctgcacgg ccagccatcc tgctgcaacg
    75841 caacgtccag cgggcgagag gtgtaatcca tcttgccggg cgtcatgtct gcacccggtg
    75901 ttgacgccgt taccagcgtg cgcgtgggca gtgaaagccc ttccaccggc actgcgcgca
    75961 aggcgtttaa ctgcgcgcga aaaccgggcg tagaggcatt ggccagatta ctggcggtta
    76021 ccgcctgttg attcagcgtc tggctggctg ctcccatcgc agtataaatt gcgtgatcca
    76081 ttgagctatc ccgtcagcga ttagcgtaag ttaacccgcg tgttgaggat ctggtcctgg
    76141 gttttgatgg tctgggcgtt agacttatag ttacgctggg caacgatcat attgaccagt
    76201 tctttactga gatcgacgtt ggacgcttcc agcgcaccgt tggtcagagt gccaaagttt
    76261 cccgtcccgg ctgtccccaa cagcgccacg ccagaagatt gcgtcgcaga ccagacgttg
    76321 tcgccttcgg atgccagacc ttcgttgttg gcaaagttcg ccagtacaat ctgccccagc
    76381 agttgggttt gttcgttgga attgttgccg acaaccgtac cgtcatcatt gatttgataa
    76441 ctcaccagat cgcccggttt gtagccattc tgggtggttg ccacaatgtt gttagcgccg
    76501 gttgcgatat tattcgccat cgcaccatcc actaatgtgc cattagcatt aaattccagt
    76561 gttgtcgctg tcttcgcaat gctgtttgga tcactgctat cctgggtgta gacctgccag
    76621 ttattatccc cggtcttcac aaaatagacg ctcatgtcat gagcattacc ctgactgtcg
    76681 aaaacagtca ccgaaccttt tttgttatag ctatccgcat tgctggcgct gaatggcgta
    76741 acagtaggaa gcggatcact ggaattcagg ttgatctgca tcgacgccgt ggtggtagtt
    76801 ttcgctgcca tcagggtatt cgggatcgaa atattggtcg gattcgcccc ttgctgaata
    76861 gtcggcggcg taccggttac cgggtaaccc gtcagctgta aaccttgcgt attcaacagg
    76921 ttacggtttt catccagctt aaattgtccg ttacggctgt agaacaccga accgttgctg
    76981 tctaccagac ggaaaaaacc gttctggctg atagcaacgt ccagacctcg cccggtgttg
    77041 gtggtcgtgc catcggtaaa gtcctgagtg ataccggcaa cttttacccc cagtcccact
    77101 ttcgaaccgg caaacatatc ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg
    77161 gagttggcga tattgttgcc aataacatcg aggttggtgg cggcagcgtt taatccgcta
    77221 accgcttgag aaaaggccat gactgactcc tgaaatgtga aggcttaaat tatctgccgt
    77281 acttcgtcga gggtggtggt gccgtaagtg ccgagatcca gcgtattacc gctgttgccg
    77341 cggatcacac cctgcaccag agcaaactgc agcggctggg caaccagttg tgtaccaccg
    77401 ttactggcgc taatcgctac attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac
    77461 gaaccgtccc aggtgaaact gtgaactccg gcggtcagtt caccaatatc aatggtgcgc
    77521 acaaccgcgc catttttatc ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc
    77581 tcaacaccaa acggcgtggt cgtggtcaca gccccttctt cactgccggt tcccgcaaga
    77641 acagttgtgc cggggatcat cacgccgtga ccgatcaggt tactggcctg taacgactgg
    77701 ctgttatcaa tctgtccgga aatagatccg agcgtggtat tgagtttttc aatcccgctg
    77761 accgtgctga tttgtgccaa ttgcgacgtc agctcgttgt tttccattgg attggtcggg
    77821 tcctggtttt tcagctgcgc caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg
    77881 ttgctgcccg tgagcgaact actgctggtg gtactgacgc cggtatttgt cggatcggtg
    77941 gtggttaccg caatggacat agctttctcc tttattgacc gagcgtaagg gttttcagca
    78001 tcatgctttt caccgtgttg agcacttcaa cattggcctg atagctgcgt gacgccgaca
    78061 tggtgttaac catctccccg acgacatcaa cgttcggcat ttttatgtag ccctttgcat
    78121 ctgccagcgg attacctggt tcataaacca gtttgtccgg ggcctgactt tcttaacatc
    78181 ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg ttaacctgga ataccacctg
    78241 ttttgcccga tatggctgtc catcaggacc ggtcacgcta tcagcatttg ccagattact
    78301 ggccgccacg ttcaggcgct gggactgggc agttaacgcc gacccggcga tatcaaaaat
    78361 attcagcagt gccatccgtt aatttccgct ctgtaaaacg ttcatcatgc ctttgatttg
    78421 cccgctcaac gcgctaaggc tcatctggta ttgcaggctg ttatcggcaa actgggtgcg
    78481 ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc tggtccggaa tacggtattg
    78541 cagttctgcg gtaggaggcg tcagcgcctg cgccgggatg tgctgcgttg aggtcatcgt
    78601 cagtgcaacc acactggttg catcccgtcc acgttgcatg acttttttaa gttcactggc
    78661 aaaatcgata tcgcgcgcct gataaccagg ggtatcggca ttggcgatgt ttgctgccag
    78721 cacttcctga cgctgggcgc gcagattgag cgcctcttgt tgaaaacgta aggcggcgtc
    78781 gagcttatcg agcatatctc ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct
    78841 ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc gctatttatg ccgttgatgg
    78901 tcattgcgga caggtacaat tcacgttgta gaaatggctg ggggcgaaaa tgctgacaat
    78961 aaaacgtagc gtggcgatca tcgcgatact gttcagtccg ttaagtacgg cgagcaatct
    79021 cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg ggggtaagtg atgaggttcg
    79081 tgtttctatt cgtacagcgc ccaatctact accgccatgc gagcagccat tgctttcgat
    79141 gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg gcacgctgcg gtaacgacaa
    79201 acgatattta caggttaatg tgcaggccac aggaaattat gtggttgccg cgatgcctat
    79261 tgtgcgggga ggaaagctgg aagctggcaa tgtcaaactg aaacgtggac gcctggatac
    79321 cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt gatgccgtta gcctgcgcga
    79381 tctatcaccc gatcaaccta tccagttaac ccagtttcgc caggcatggc gggtaaaagc
    79441 gggacaacgc gtcaatgtga tcgccagcgg tgatgggttt agcgccaacg cagaaggtca
    79501 ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg cgcatggtat cggaacaggt
    79561 agtcagcggc gttgttgatg cagatgggaa tattcttata aacctgtaag ctgttaaaga
    79621 ttacccgtcc cttgccgata aataagcaac acatgataaa agcgccctca atgaggaata
    79681 aaccatgagt attgatcgca cttcgcctct gaagcctgta agcaccgttc aaccgcgcga
    79741 aaccaccgac gcgccggtaa cgaacagccg ggcggcaaaa acaaccgcct ccaccagcac
    79801 cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa
    79861 tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac ggtgaactaa aaatggacac
    79921 cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa gacttgcaga gtaactgatc
    79981 gtatgacacg tcttgcagag atcctcgacc agatgtccgc tgtgcttaac gatctcaaaa
    80041 cggtaatgga tcaagagcag caacatctct ctatggggca gatcaacggc agccagttgc
    80101 aatggattac agaacaaaaa agctcactgc tggcgacgct ggattacctc gaacagttac
    80161 gcaagaaaga acccaataca gcaaatagcg ttgatattag tcaacgctgg caggaaatta
    80221 ctgtgaaaac gcagcaacta cgccaaatga atcaacataa cggctggtta ctggaaggac
    80281 agattgagcg caatcaacag gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctat
    80341 atggggcgaa cggtcagacc tcaacacccc atcgcggcag taaaaagatt tcgatctgaa
    80401 tattatctcc ggcctgcact gcaggccgga atgcattgtt acaccgtccg gcgggcaaat
    80461 tctttaactt tgaagcccag taccgccagt gcagcgaagt acgcggcaat ccccgccagc
    80521 acgaccgcca ttaaacgcag taagcgccag ggcatggtac ccaatgacca ctccggcatg
    80581 atatgtaaca tacctaaaag cacgccagac atcaccagta ccgccaccac cagacgcaac
    80641 agaaacgcca tccagccggg ttgcggagta aagattttct gcttacgcaa ctgccagtaa
    80701 agcagcgaag cattcagaca cgccgccaga ccaatagaaa gtgacagccc ggcatgtttc
    80761 aacggaccaa taaacgccag gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt
    80821 ttcactggcg ttttaatgtc ctggcgggaa taaaagccag gagccaacac tttcactaca
    80881 atcaggccaa tcaaacccac cgagtaggca attaacgccc gctgggtcat cagcgcatca
    80941 aacgcggtaa atttaccgta ctggaacagc gaaacggtta acggacctga aagaatgccc
    81001 aacgcaaccg cactcggcag tgccaacagg aaacaaagac gcaaccccca gtccatcaaa
    81061 cggttgtatt catcatgatt gccactggca aaacttttcg acagcgacgg cagcaaaatg
    81121 gtgccaagcg ccacgcccag cacaccggag ggaaactcca ttaagcggtc ggcgtaatac
    81181 atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa tggtgttgat gattaaggag
    81241 atctggctca cagagacgcc aaggatcgcc ggtcccatct gtttcaccac gcgcattgct
    81301 ccagcatcgt ggaagttaat gcgcggcagg accagcatgc cgatcttctt caggtgcggt
    81361 agctgataca ccagctgcag aatgccgccg accgtaacag cccacgccag cgccagcacc
    81421 ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa tcatgctgat gttaagcagt
    81481 gttggcgcaa acgccggaat cgagaagcgg ttccacgtat tcagaatcgc tcccaccagc
    81541 gacgccaggg agatcagcaa gatataggga aaggtaatct ttagtagctg gctggtcagg
    81601 gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca ccatgatcac ccacggtgcg
    81661 gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg tcagcaggcc agaaacataa
    81721 gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt tatattccgc cagaatcggt
    81781 acaaatgcct gggaaaatgc cccttcggca aagatacggc gtaacaagtt aggaagttta
    81841 aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa agattctggc gacaattgcg
    81901 tctcgtgcga agccaagcac acgcgaaaac atggtcatcg agctgacggc ggccagcgat
    81961 tttaataaat tcatcggtgt tctaatccgg acccacgggc aaaacgcctg caaggcaggc
    82021 gctgaaagag gcgaatagtc tacctagatt atggtgaatt gctaccgcca gatgttacag
    82081 ggtttattca ctcatcgcat cgcgccagat cttgtcaacg atacgttgcg ccagcactgc
    82141 ctattcgccg gcggtttgcg gaactgtctg attttacacg cattcaatga agtgccgcgc
    82201 acagccgaca aatccacgtt gctcaagagt gctctgccaa ccaggaatcg gtttattcac
    82261 tacaccctgc ccgcgctcct cacgccattc gcgcatatcc gtaatgtcga tgagcgcacc
    82321 gtcagtcacg gcctgcacgg tttcacgctg acttccggca cggcgatgca tacaagtggt
    82381 gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac agcatttcgc cagcgtcgtt
    82441 agtcagtagc gtaccgccat ccagagaggc tttgccgccc gacaaccaca gcgcggtatc
    82501 caccacatgc agataatcat ccagcaacgt gaaataaaga tcgtgtggcc cgacgctatt
    82561 gctacgatgt ttatccattc ttagcgaggc tgcggtggcg agttgcgttt ttaactcacc
    82621 gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac gtcagttttt tccgcgccgc
    82681 cagttccacc agcctttcag catcgcgcag attttctgcc agcggtttat cgacacagac
    82741 atgtaccccc gcattgagta acgtactgac cacgtcaaag tggctggcgg tgctggagtg
    82801 cacaaaaacc gcatcacaac tggcggcaag gctggataac gaatcggcat aaggaatgcg
    82861 ccagctgtca caaattggca gggctttggc gcgcgtaggc gaccaggctc cttgtaacgt
    82921 ccagtcagac gctgccgcca gcaccggtaa ccacgctttt tgcgcaatgc cgcctaatcc
    82981 cactacgccg atacgtaatt ttttcacttt aatctcccag atgggccagc aacgaatcaa
    83041 gacgttgttt cagttctgcc acttcgattt ccagggcttc gacgcgggtc tgtaaatcac
    83101 cgtcaaccat gttcgacata gccgttaccg ccggctgatc ttcaacctca ccactgaaaa
    83161 gatgcatgta gcggctttcg cgtttacccg gttcgcgggc cagacgcacc acaaaaggac
    83221 catcttcgcg attcgccagt tgttccagcg tcgactccac ttccgccata tcgctgaatt
    83281 catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt ctgggcacca cgcaataaca
    83341 acgtggtgat taacgccact tctgcggcgc tcagtttcag atcgccaaat tctgaattac
    83401 aaaaacgttg ctcatatttg gtgacccgat taccaaaacc gctcactgtg cgtagataat
    83461 gacgtttgac cagattatcc agctgttcct gcacttcgga ttcactcaga ttcatgaccg
    83521 gttcacggtt cgttttctga ttacaggccg tgactacacc attgactgag agcgggtatt
    83581 gctccggcgt ggtcacctgc ttttccagca agcaaccaat tacgcgagct tccagggcag
    83641 taagttgata tttcatgaga ttctccttag cggccgggcg tccagtctgg ggtagttaat
    83701 gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca gatagtcttt cgcatagcct
    83761 tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc gtttattgtg cggcatataa
    83821 ttagccataa tgcgatgaat atgttgggtg cgctgcatat aacgaatggc tgcggtcagg
    83881 gcttcaaaca tgagtccttt gccctgccat ttttgcccaa tcgaataacc gagatagcag
    83941 gcatgaaaag agccgcgaac aacattggaa aaattggcaa cgccaataat ctctttttcg
    84001 tccgggtcga ataagccaaa gtagaaagct gaaccttgtt tatgaaattc gttaatcatc
    84061 cctagcctgg cctgccagcc tgatggataa cagtggcttt cgtcgcgcac tggctcccag
    84121 ggcttgagga aatggcgatt ctctgcgtaa taatccgcca gacgccaggc atcacgatca
    84181 tgcaccagac gcacgaccag tcggtctgtg gttaagcgca cttttggcac gttactgcga
    84241 tagccaaaca ttctatacct actccttccc gtaacgtctt taatacgctg taagtattac
    84301 tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc cattttctgc attattcaca
    84361 tatttgataa aaactctcta tcaaagcgac gttttgataa aaaaatactg tccccgaagt
    84421 gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc tcatctttta tttcccggga
    84481 ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta aatatttcct gctcatcgat
    84541 aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt
    84601 gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc tcggtctacg ccagtttatt
    84661 cagcaaggtc tgggtatttt cggcggcgca attgccgacc gctttggtgc caaaccgatg
    84721 attgttaccg gtatgctgat gcgcgccgcc ggattcgcca caatgggtat cgcccacgaa
    84781 ccgtggctat tgtggttttc atgcctgctc tcgggactcg gcggcacgtt gtttgatccg
    84841 ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac aacgtggtcg ttttttctcg
    84901 ctgttgatga tgcaggacag tgccagtgcg gtcattggcg cattgttggg gagctggctg
    84961 ttgcaatacg actttcgcct ggtctgcgcc acaggggccg ttttgtttgt gctatgtgcg
    85021 gcgttcaatg cgtggttgtt accggcatgg aaactctcca ccgtacgcac gcccgttcgc
    85081 gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca cctatgtcct gacgctggcg
    85141 ggttactaca tgctggctgt acaagtgatg ctgatgctgc caattatggt caacgacgtg
    85201 gctggcgcgc cctctgccgt taaatggatg tatgccattg aagcgtgtct gtcgttaacg
    85261 ttgctctacc ctatcgcccg ctggagtgaa aagcattttc gtctggaaca ccggttgatg
    85321 gctgggctgt tgataatgtc attaagcatg atgccggtgg gcatggtcag cggcctacaa
    85381 caacttttca ccctgatttg tctgttttat atcgggtcga tcattgccga gcctgcgcgt
    85441 gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg gcagctatat ggggtttagc
    85501 cgtctgggtc tggcgattgg cggcgctatt ggttatatcg gtggtggctg gctgtttgac
    85561 ctgggcaaat cggcgcacca gccagagctt ccgtggatga tgctgggaat tattggcatc
    85621 ttcactttcc ttgcgctggg ttggcagttt agtcagaaac gcgccgcgcg tcgtttgctg
    85681 gaacgcgacg cctgatttga tactcccccc tgactcctcc atactgaaag tgaactgaca
    85741 gccaaatgga ggagtcacgt gaagctatac atttacgatc actgccctta ctgcctcaaa
    85801 gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat tacatgttct gctcaacgac
    85861 gacgcagaaa cacccacccg gatggtcggt caaaaacagg ttcccattct gcaaaaagat
    85921 gacagccgct atatgccaga aagcatggat atcgttcact atgtcgataa actcgacggc
    85981 aaaccgttac tgaccggcaa acgttcccct gccattgaag agtggctgcg caaggtcaat
    86041 ggctacgtca acaaactgct gttgccgcgt tttgccaaat cggcatttga tgagttttct
    86101 actcccgccg cgcgcaaata tttcgtcgac aagaaagagg ccagcgcggg taattttgcc
    86161 gacctgctgg cccactctga cggtctgatt aagaatatca gcgatgattt gcgtgcgctg
    86221 gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac tttcggaaga tgatattcag
    86281 ctatttccgc tactgcgtaa tctgacgctg gtagccggaa tcaactggcc aagccgcgtt
    86341 gctaattacc gcgataatat ggcgaaacag acacaaatca atttgttatc atcaatggcg
    86401 atttaatcct gacccgccgc ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt
    86461 tctgctggcg agtttatcgc tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga
    86521 tttcatgtcg aggactacca tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg
    86581 cctgctcgtt ggctgtaatc aactcaccca atacaccatc accgaacaag aaattaacca
    86641 gtcgcttgcg aaacataata atttctcaaa agatatcggt ttacccggcg tggctgacgc
    86701 ccatattgtt ctgacaaact tgaccagcca aattggtcgc gaagagccga ataaggttac
    86761 cctaaccgga gacgccaatc tggacatgaa ctccctgttc ggtagtcaga aagcgaccat
    86821 gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa
    86881 agagatggaa gtggtcgatg cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac
    86941 gttgcttccc tatttgaacc aggcattacg caattacttt aaccagcaac ctgcttacgt
    87001 cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa aaactggcga aaggcattga
    87061 agtgaagcca ggcgaaattg tcattccgtt tacggattaa tgacgagggc gcattcgcgc
    87121 cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat
    87181 cccttcagcc ggagcataga gattaatgac tgcaccatcc caggtattaa agatccgccg
    87241 cccagacgac tggcaccttc acctccgcga tggcgacatg ttaaaaactg tcgtgccgta
    87301 taccagcgaa atttatggac gggctatcgt aatgcccaat ctggctccgc ccgtgaccac
    87361 agttgaggct gccgtggcgt atcgccaacg tattcttgac gccgtacctg ccgggcacga
    87421 tttcacccca ttgatgacct gttatttaac agattcgctg gatcctaatg agctggagcg
    87481 cggatttaat gaagccgtgt tcaccgctgc aaaactttac ccggcaaacg caaccactaa
    87541 ctccagccac ggcgtgacgt caattgacgc aatcatgccg gtacttgagc gcatggaaaa
    87601 aatcggtatg ccgctactgg tgcatggtga agtaacacat gcagatatcg acatttttga
    87661 tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg cgccagcgcc tgactgcgct
    87721 gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc gactatgtcc gtgacggaaa
    87781 tgaacggctg gctgccacca tcactccgca gcatctgatg tttaaccgca accatatgct
    87841 ggttggtggc gtgcgtccgc acctgtattg tttacccatc ctcaaacgca atattcacca
    87901 acaggcattg cgtgaactgg tcgccagcgg ttttaatcga gtattcctcg gtacggattc
    87961 tgcgccacat gcacgtcatc gcaaagagag cagttgcggc tgcgcgggct gcttcaacgc
    88021 cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag atgaatgctc tgcagcactt
    88081 tgaagcattc tgttctgtaa acggcccgca gttctatggc ttgccggtca acgacacatt
    88141 catcgaactg gtacgtgaag agcaacaggt tgctgaaagc atcgcactga ctgatgacac
    88201 cctggtgcca ttcctcgccg gggaaacggt acgctggtcc gttaaacaat aaaaatcgta
    88261 gcttcctgtt gtcattaggt tattttacct gtataaataa ccagtatatt caacaggggg
    88321 ctattatgcg aattgaagtc accatagcga aaacttctcc attgccagct ggggctattg
    88381 acgccctggc tggcgaactt tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc
    88441 acgtatcggt acgttatgcc gcagcgaata atttatcggt tattggcgca acaaaagaag
    88501 ataaacagcg cattagcgaa attctccagg aaacgtggga aagcgccgat gactggtttg
    88561 tcagcgaata atatgcagtg atttttgttt ttgccggatc gccccggctt ttttatttta
    88621 cttacgaatt ttgagtacaa aatttctaca cttcctcaaa ataattgcac aatatgctaa
    88681 aaaaatatga atgacatgct gttttattgt tcatataaat cttaaaagca gaaatttatc
    88741 tgttcccccc tttaaaaatt cggtgaatac ccttacttat tggtatactg aagttgcttc
    88801 agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta gggggccatg
    88861 atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga catcagcacc
    88921 gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa taagtccgag
    88981 caggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc cagacaattt
    89041 atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc ggtaaacgag
    89101 tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc ggtgccttta
    89161 ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca tgagagcaga
    89221 gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg cgctgccgcc
    89281 gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca tatgccaccg
    89341 catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta tggtgaaggc
    89401 gaaaagtatg tcccgttggt cctccgcgcg caaacgctgt gggatgaact ttcccgccac
    89461 aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc ggctgactcc
    89521 gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt tgaaaagctc
    89581 gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa ctacatcggc
    89641 ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac ctggatccaa
    89701 ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc aattcgtcat
    89761 gacgatgatg gcgtaactat tgaaacggtt gacggtgagt atcaggcgaa aaaagcgatt
    89821 gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca gcccgtacgt
    89881 aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa attcccggcg
    89941 tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga aaacgacgcg
    90001 ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga acgtgttccg
    90061 tttgcggaag tggccagtga tgggtcagaa gccttcccgt tcttgcgcaa tgtattgccg
    90121 ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc tgacgaagat
    90181 tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg cctgagtggg
    90241 cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc gcaagaccaa
    90301 aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc atcattacgg
    90361 cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg cactatctca
    90421 tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg ttggcaatta
    90481 tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact gacaaatatt
    90541 taatctccct ttcatttagc gtgaaaaatt gaatgagatt attcctgctt ctacagcact
    90601 caccagacat ctctgatgct gaacccacta aacttatgaa gattctttca ttattaattc
    90661 aaaattattg aaataaaaat aaatatcatt caatttgtta tttttgtgtt gcaattcatt
    90721 ctccgacacg ccattttctt tctctcgcaa attcgtattg gatgcactta tgtcattcac
    90781 aaatacccct gaacgctatg gtgttatatc tgcggccttc cattggctaa gcgcaattat
    90841 tgtctacggc atgtttgctt taggtctgtg gatggtcacg ctcagttatt acgatggctg
    90901 gtatcacaaa gcacccgaac tgcataaaag catcggtatt ttgttaatga tggggctggt
    90961 tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt ccgctgccga gttattcgcc
    91021 catgactcgt cttgccgcaa aagctggaca tctcgcattg tgcctattgc tgttcgccat
    91081 cggcatcagc ggctatctga tctcaactgc cgatggtaaa ccgatcagcg tttttggctg
    91141 gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag gcagactttg ccggtgccct
    91201 gcatttttgg ctcgcgtgga gcgtcgtagt actgtctgtt atgcacggat ttatggccct
    91261 gaagcatcat ttcatcgata aagacgacac tctgaagcgc atgctgggaa agtcgtcatc
    91321 tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg cgtccctgat
    91381 gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag gtcagcacgc
    91441 ctttgttaac ttccgcatac agcatcttgg ctatagctgg ttatatggca cctttaaaga
    91501 tttcgacggt acttttacct ttgatgaaaa aaatccggct gccgataaag tgaatgtgac
    91561 aattaacacc accagcgtcg atactaacca cgccgaacgc gataaacatc ttcgcagtgc
    91621 ggattttctc aataccacaa aatatccaca ggcaacattc acctccacca gcgtgaagaa
    91681 agacggtgac gaactggata ttaccggcga tctgacgctg aatggcgtaa ccaaacccgt
    91741 cacgctggaa gcgaaattaa ttggtcaggg cgacgaccca tggggcggta aacgtgcagg
    91801 ctttgaggcc gaaggcaaaa ttaagctcaa agacttcaat atcaagacag atttaggtcc
    91861 agcttctcag gaagtggatc tgattatttc agtggaaggc gtacagcaga agtaagagtg
    91921 aaatgttgcc ggatggcacg catccggcaa tgatatttat tctgttggat caggaatacc
    91981 cagtgttgta ttcagacgtc cacgagactt attaaagatc ttattgccat tttcacgtcc
    92041 tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc aaatctcact
    92101 acaacaacct ttgtattttt ccgcgcatac tggacactga ataaacagca ggtggcagcc
    92161 atcattttta cagttggtat ggctgtcgca cggcgcgccg cactggtggc aatgcgcgat
    92221 aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt tgccaataaa
    92281 acgcaccggc aagccctgct cgcgcgcctt acgggcgtat tcaataattc cgccctcgat
    92341 atgccagact ttgttgaatc cgttatgttt catccaggca ctggcctttt cacaacgaat
    92401 gccgccggtg cagtacatga cgattttttt atctttatgt gcctgcatca tctcgactgc
    92461 ttttggcagc tgctcacgga aggtatctgc cggaatttcg agcgcgtttt caaagtgccc
    92521 cacttcatac tcatagtggt tacgcatgtc gataaatagt gcatcgggat cgtcaagcat
    92581 ggcgttcact tccgccgctt gcagatactc accaacattg ctggcatcaa agtgaggatc
    92641 gtcaataccg tcggcaacaa tgcgatcgcg taccttcatg cgcagtaccc agaaggattt
    92701 cccgtcatca tccaacgcga tattcaggcg taaaccctct aaagccgggt cgaaggcata
    92761 gagctgcgcg cgaaatgttt caacattgct cgcaggtacg ctgatttgcg cgttaatgcc
    92821 ctcatgcgcc agatacactc gcccaaaaac attcagcgcg gtaaacagct gatataaagc
    92881 gtcacgggtc gccttaggat cggcgatgtg gaaatacttg taaaacgaaa tggtggttcg
    92941 cggttcgctc tcagccaaca ttttggcttt taacgcgtcg ttggaaatgc ggttgtgtaa
    93001 cactggcatg gtgtacggtt cctgcgagat gggaaagtaa aaatccgcgg catgatatag
    93061 caattatcga taattaacat ccacacattt tacgctacat ttgcgcatta aaaattattt
    93121 gttatttaca agcgcggcaa tttcgcccag tcttcagcca caaattttgg ttgcgggcga
    93181 aaaaatgcga caatacatac aattgcccgt ataggttgaa aaacaggatt gatatgacga
    93241 atctacccaa gttctccacc gcactgcttc atccgcgtta ttggttaacc tggttgggta
    93301 ttggcgtact ttggttggtc gtgcaattgc cctacccgat tatctaccgc ctcggttgtg
    93361 gattaggaaa actggcgtta cgttttatga aacgacgcgc aaaaattgtg catcgcaacc
    93421 tggaactgtg cttcccggaa atgagcgaac aagaacgccg taaaatggtg gtgaagaatt
    93481 tcgaatccgt tggcatgggc ctgatggaaa ccggtatggc gtggttctgg ccggaccgcc
    93541 gaatcgcccg ctggacggaa gtgatcggca tggaacacat tcgtgacgtg caggcgcaaa
    93601 aacgcggcat cctgttagtt ggcatccatt ttctgacact ggagctgggc gcgcggcagt
    93661 ttggtatgca ggaaccgggg attggcgttt accgcccgaa cgataatcca ctgattgact
    93721 ggctacaaac ctggggccgt ttgcgctcaa ataaatcgat gctcgaccgc aaagatttaa
    93781 aaggcatgat taaagccctg aaaaaaggcg aagtggtctg gtacgcacca gatcatgatt
    93841 acggcccgcg ctcaagcgtt ttcgtcccgt tatttgccgt tgagcaggct gcgaccacga
    93901 ccggaacctg gatgctggca cggatgtccg gcgcatgtct ggtgcccttc gttccacgcc
    93961 gtaagccaga tggcaaaggg tatcaattga ttatgctgcc accagagtgt tctccgccac
    94021 tggatgatgc cgaaactacc gccgcgtgga tgaacaaagt ggtcgaaaaa tgcatcatga
    94081 tggcaccaga gcagtatatg tggttacacc gtcgctttaa aacacgcccg gaaggcgttc
    94141 cttcacgcta ttaaatctcc catgccggat gcttcagaat ggcatccggc attaccacag
    94201 caaatccccc tgatttagcg ataaaagctc tctggattgc gccccctgga agtcgggcgc
    94261 ataattagtg tgtttatctt ttcttcttat gttcaccgcg cctggcgcac caacagcgga
    94321 ttgctatgtc accctgtgaa aatgacaccc ctataaactg gaaacgaaac ctgatcgtcg
    94381 cctggctagg ctgttttctt accggggccg ccttcagtct ggtaatgccc ttcttacccc
    94441 tctacgttga gcagcttggt gttaccggcc actccgccct gaatatgtgg tccggtattg
    94501 tcttcagtat aatgggttcc taaacacatc attttaggta ttgaatatga gacgggcaat
    94561 cagctacatc agattttcaa gtgaacgaca actgaaaggg gactctgtac gccgacaatc
    94621 taagctggtt actgactggc ttgataagaa ccctgagttt tacttggatt catccttgag
    94681 ctttaaagat ttggggaaaa gtgcttttag tggtaagcac ctgaaaggtg gtctaggtga
    94741 ctttttaacc gctatcgaga aagggcttgt taaagctggt gatacgctac taatcgagag
    94801 tttagaccgt ctgtcacgtc aggatattga tattgcttct gagctattac gccgtatctt
    94861 aagggctggt gtagatgtag tcacactgag cgatggagaa cattacacac gagaaagttt
    94921 aaaagacccg ttagccctga ttaaaagcat tctgataatg cagcgggcgc atgaggaaag
    94981 tttgagaaag tctgagcgtg tccaagctgc atggaaccgt aaaaaagaac tcatctccga
    95041 aggtatcaag gtatctaggc gttgtcctgc atggttaagg ctgaatgatg accgtcgaac
    95101 attcacaatc attcctgata aagtggaagt tgttaaacga gcatttgacc tgagattaca
    95161 gggtttaagc t
//
LOCUS       DAJTUP010000002        93220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTUP010000002 DAJTUP010000000
VERSION     DAJTUP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 93220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.002"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            102..227
                     /locus_tag="OO468_000107"
     ncRNA           102..227
                     /ncRNA_class="other"
                     /locus_tag="OO468_000107"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            347..688
                     /locus_tag="OO468_000108"
     CDS             347..688
                     /locus_tag="OO468_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR6257369.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(679..1605)
                     /locus_tag="OO468_000109"
     CDS             complement(679..1605)
                     /locus_tag="OO468_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6257370.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1695..2693
                     /locus_tag="OO468_000110"
     CDS             1695..2693
                     /locus_tag="OO468_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR6257371.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2690..2908)
                     /locus_tag="OO468_000111"
     CDS             complement(2690..2908)
                     /locus_tag="OO468_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR6257372.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2910..4925)
                     /gene="ligA"
                     /locus_tag="OO468_000112"
     CDS             complement(2910..4925)
                     /gene="ligA"
                     /locus_tag="OO468_000112"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR6257373.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(4996..5982)
                     /gene="zipA"
                     /locus_tag="OO468_000113"
     CDS             complement(4996..5982)
                     /gene="zipA"
                     /locus_tag="OO468_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR6257374.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6212..6973
                     /gene="cysZ"
                     /locus_tag="OO468_000114"
     CDS             6212..6973
                     /gene="cysZ"
                     /locus_tag="OO468_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR6257375.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7158..8129
                     /gene="cysK"
                     /locus_tag="OO468_000115"
     CDS             7158..8129
                     /gene="cysK"
                     /locus_tag="OO468_000115"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR6257376.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8513..8770
                     /gene="ptsH"
                     /locus_tag="OO468_000116"
     CDS             8513..8770
                     /gene="ptsH"
                     /locus_tag="OO468_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR6257377.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8815..10542
                     /gene="ptsI"
                     /locus_tag="OO468_000117"
     CDS             8815..10542
                     /gene="ptsI"
                     /locus_tag="OO468_000117"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR6257378.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10583..11092
                     /gene="crr"
                     /locus_tag="OO468_000118"
     CDS             10583..11092
                     /gene="crr"
                     /locus_tag="OO468_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR6257379.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11134..11985)
                     /gene="pdxK"
                     /locus_tag="OO468_000119"
                     /gene_synonym="thiJ"
     CDS             complement(11134..11985)
                     /gene="pdxK"
                     /locus_tag="OO468_000119"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR6257380.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12090..12464
                     /locus_tag="OO468_000120"
     CDS             12090..12464
                     /locus_tag="OO468_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR6257381.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            12497..13220
                     /locus_tag="OO468_000121"
                     /pseudo
     CDS             12497..13220
                     /locus_tag="OO468_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(13424..14335)
                     /gene="cysM"
                     /locus_tag="OO468_000122"
     CDS             complement(13424..14335)
                     /gene="cysM"
                     /locus_tag="OO468_000122"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR6257382.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(14469..15566)
                     /gene="cysA"
                     /locus_tag="OO468_000123"
     CDS             complement(14469..15566)
                     /gene="cysA"
                     /locus_tag="OO468_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR6257383.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(15556..16431)
                     /gene="cysW"
                     /locus_tag="OO468_000124"
     CDS             complement(15556..16431)
                     /gene="cysW"
                     /locus_tag="OO468_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR6257384.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(16431..17264)
                     /gene="cysT"
                     /locus_tag="OO468_000125"
     CDS             complement(16431..17264)
                     /gene="cysT"
                     /locus_tag="OO468_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR6257385.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(17264..18280)
                     /gene="cysP"
                     /locus_tag="OO468_000126"
     CDS             complement(17264..18280)
                     /gene="cysP"
                     /locus_tag="OO468_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR6257386.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(18584..19375)
                     /gene="ucpA"
                     /locus_tag="OO468_000127"
     CDS             complement(18584..19375)
                     /gene="ucpA"
                     /locus_tag="OO468_000127"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR6257387.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(19562..20362)
                     /gene="murR"
                     /locus_tag="OO468_000128"
     CDS             complement(19562..20362)
                     /gene="murR"
                     /locus_tag="OO468_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR6257388.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            20526..21422
                     /gene="murQ"
                     /locus_tag="OO468_000129"
     CDS             20526..21422
                     /gene="murQ"
                     /locus_tag="OO468_000129"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR6257389.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            21426..22850
                     /gene="murP"
                     /locus_tag="OO468_000130"
     CDS             21426..22850
                     /gene="murP"
                     /locus_tag="OO468_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR6257390.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(23030..23929)
                     /gene="yfeX"
                     /locus_tag="OO468_000131"
     CDS             complement(23030..23929)
                     /gene="yfeX"
                     /locus_tag="OO468_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR6257391.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(24025..24600)
                     /locus_tag="OO468_000132"
     CDS             complement(24025..24600)
                     /locus_tag="OO468_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR6257392.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(24661..25110)
                     /locus_tag="OO468_000133"
     CDS             complement(24661..25110)
                     /locus_tag="OO468_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR6257393.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(25097..25522)
                     /locus_tag="OO468_000134"
     CDS             complement(25097..25522)
                     /locus_tag="OO468_000134"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR6257394.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            25736..26605
                     /gene="amiA"
                     /locus_tag="OO468_000135"
     CDS             25736..26605
                     /gene="amiA"
                     /locus_tag="OO468_000135"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR6257395.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            26609..27508
                     /gene="hemF"
                     /locus_tag="OO468_000136"
     CDS             26609..27508
                     /gene="hemF"
                     /locus_tag="OO468_000136"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR6257396.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(27514..28566)
                     /gene="eutR"
                     /locus_tag="OO468_000137"
                     /pseudo
     CDS             complement(27514..28566)
                     /gene="eutR"
                     /locus_tag="OO468_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(28612..29112)
                     /gene="eutK"
                     /locus_tag="OO468_000138"
     CDS             complement(28612..29112)
                     /gene="eutK"
                     /locus_tag="OO468_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR6257397.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(29125..29784)
                     /gene="eutL"
                     /locus_tag="OO468_000139"
     CDS             complement(29125..29784)
                     /gene="eutL"
                     /locus_tag="OO468_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR6257398.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            30966..31757
                     /locus_tag="OO468_000140"
     CDS             30966..31757
                     /locus_tag="OO468_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257399.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            32477..32872
                     /locus_tag="OO468_000141"
     CDS             32477..32872
                     /locus_tag="OO468_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6257400.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            32890..33153
                     /locus_tag="OO468_000142"
     CDS             32890..33153
                     /locus_tag="OO468_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257401.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            33176..33718
                     /locus_tag="OO468_000143"
     CDS             33176..33718
                     /locus_tag="OO468_000143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257402.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            33857..34144
                     /locus_tag="OO468_000144"
     CDS             33857..34144
                     /locus_tag="OO468_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257403.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            34183..34389
                     /locus_tag="OO468_000145"
     CDS             34183..34389
                     /locus_tag="OO468_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257404.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            34701..35186
                     /locus_tag="OO468_000146"
     CDS             34701..35186
                     /locus_tag="OO468_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6257405.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            35167..35445
                     /locus_tag="OO468_000147"
     CDS             35167..35445
                     /locus_tag="OO468_000147"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257406.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            35438..36898
                     /locus_tag="OO468_000148"
     CDS             35438..36898
                     /locus_tag="OO468_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6257407.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            36932..37741
                     /gene="murI"
                     /locus_tag="OO468_000149"
     CDS             36932..37741
                     /gene="murI"
                     /locus_tag="OO468_000149"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6257408.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(37860..38554)
                     /locus_tag="OO468_000150"
     CDS             complement(join(37860..38308,38308..38554))
                     /locus_tag="OO468_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6257409.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(39073..40446)
                     /gene="eutA"
                     /locus_tag="OO468_000151"
     CDS             complement(39073..40446)
                     /gene="eutA"
                     /locus_tag="OO468_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR6257410.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(40421..40900)
                     /gene="eutP"
                     /locus_tag="OO468_000152"
     CDS             complement(40421..40900)
                     /gene="eutP"
                     /locus_tag="OO468_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR6257411.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40913..41248)
                     /gene="eutS"
                     /locus_tag="OO468_000153"
     CDS             complement(40913..41248)
                     /gene="eutS"
                     /locus_tag="OO468_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR6257412.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(41541..43820)
                     /gene="maeB"
                     /locus_tag="OO468_000154"
     CDS             complement(41541..43820)
                     /gene="maeB"
                     /locus_tag="OO468_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR6257413.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            44109..45059
                     /gene="tal"
                     /locus_tag="OO468_000155"
     CDS             44109..45059
                     /gene="tal"
                     /locus_tag="OO468_000155"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6257414.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            45079..47082
                     /gene="tkt"
                     /locus_tag="OO468_000156"
     CDS             45079..47082
                     /gene="tkt"
                     /locus_tag="OO468_000156"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6257415.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(47185..48228)
                     /locus_tag="OO468_000157"
     CDS             complement(47185..48228)
                     /locus_tag="OO468_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR6257416.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(48354..48929)
                     /gene="nudK"
                     /locus_tag="OO468_000158"
     CDS             complement(48354..48929)
                     /gene="nudK"
                     /locus_tag="OO468_000158"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR6257417.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(48997..50976)
                     /gene="aegA"
                     /locus_tag="OO468_000159"
     CDS             complement(48997..50976)
                     /gene="aegA"
                     /locus_tag="OO468_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR6257418.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            51182..52899
                     /gene="narQ"
                     /locus_tag="OO468_000160"
                     /pseudo
     CDS             51182..52899
                     /gene="narQ"
                     /locus_tag="OO468_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            53101..56214
                     /gene="acrD"
                     /locus_tag="OO468_000161"
     CDS             53101..56214
                     /gene="acrD"
                     /locus_tag="OO468_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR6257419.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(56313..56372)
                     /gene="ypfM"
                     /locus_tag="OO468_000162"
     CDS             complement(56313..56372)
                     /gene="ypfM"
                     /locus_tag="OO468_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR6257420.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            56753..57109
                     /locus_tag="OO468_000163"
     CDS             56753..57109
                     /locus_tag="OO468_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR6257421.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            57113..58240
                     /gene="dapE"
                     /locus_tag="OO468_000164"
     CDS             57113..58240
                     /gene="dapE"
                     /locus_tag="OO468_000164"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR6257422.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            58268..58468
                     /locus_tag="OO468_000165"
     CDS             58268..58468
                     /locus_tag="OO468_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR6257423.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(58578..59276)
                     /gene="ypfH"
                     /locus_tag="OO468_000166"
     CDS             complement(58578..59276)
                     /gene="ypfH"
                     /locus_tag="OO468_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6257424.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(59350..61365)
                     /gene="tmcA"
                     /locus_tag="OO468_000167"
     CDS             complement(59350..61365)
                     /gene="tmcA"
                     /locus_tag="OO468_000167"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR6257425.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(61380..62243)
                     /locus_tag="OO468_000168"
     CDS             complement(61380..62243)
                     /locus_tag="OO468_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR6257426.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(62411..63124)
                     /gene="purC"
                     /locus_tag="OO468_000169"
     CDS             complement(62411..63124)
                     /gene="purC"
                     /locus_tag="OO468_000169"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR6257427.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(63337..64371)
                     /gene="bamC"
                     /locus_tag="OO468_000170"
     CDS             complement(63337..64371)
                     /gene="bamC"
                     /locus_tag="OO468_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR6257428.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(64388..65266)
                     /gene="dapA"
                     /locus_tag="OO468_000171"
     CDS             complement(64388..65266)
                     /gene="dapA"
                     /locus_tag="OO468_000171"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR6257429.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            65412..65984
                     /locus_tag="OO468_000172"
     CDS             65412..65984
                     /locus_tag="OO468_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR6257430.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            65984..66454
                     /gene="bcp"
                     /locus_tag="OO468_000173"
     CDS             65984..66454
                     /gene="bcp"
                     /locus_tag="OO468_000173"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR6257431.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            66707..67324
                     /gene="hyfA"
                     /locus_tag="OO468_000174"
     CDS             66707..67324
                     /gene="hyfA"
                     /locus_tag="OO468_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR6257432.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            67324..69341
                     /gene="hyfB"
                     /locus_tag="OO468_000175"
                     /pseudo
     CDS             67324..69341
                     /gene="hyfB"
                     /locus_tag="OO468_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            69352..70299
                     /gene="hyfC"
                     /locus_tag="OO468_000176"
     CDS             69352..70299
                     /gene="hyfC"
                     /locus_tag="OO468_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR6257433.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            70316..71755
                     /gene="hyfD"
                     /locus_tag="OO468_000177"
     CDS             70316..71755
                     /gene="hyfD"
                     /locus_tag="OO468_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR6257434.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            71767..72417
                     /gene="hyfE"
                     /locus_tag="OO468_000178"
     CDS             71767..72417
                     /gene="hyfE"
                     /locus_tag="OO468_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR6257435.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            72422..74020
                     /gene="hyfF"
                     /locus_tag="OO468_000179"
     CDS             72422..74020
                     /gene="hyfF"
                     /locus_tag="OO468_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR6257436.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVL
                     MAKPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLP
                     AQRSTWHDFLPSGTASVSEKHSER"
     gene            74010..75724
                     /gene="hyfG"
                     /locus_tag="OO468_000180"
                     /pseudo
     CDS             74010..75724
                     /gene="hyfG"
                     /locus_tag="OO468_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            75734..76279
                     /gene="hyfH"
                     /locus_tag="OO468_000181"
     CDS             75734..76279
                     /gene="hyfH"
                     /locus_tag="OO468_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR6257437.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            76276..77034
                     /gene="hyfI"
                     /locus_tag="OO468_000182"
     CDS             76276..77034
                     /gene="hyfI"
                     /locus_tag="OO468_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR6257438.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            77027..77440
                     /gene="hyfJ"
                     /locus_tag="OO468_000183"
     CDS             77027..77440
                     /gene="hyfJ"
                     /locus_tag="OO468_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR6257439.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            77470..79299
                     /gene="hyfR"
                     /locus_tag="OO468_000184"
     CDS             77470..79299
                     /gene="hyfR"
                     /locus_tag="OO468_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR6257440.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            79300..79482
                     /locus_tag="OO468_000185"
     CDS             79300..79482
                     /locus_tag="OO468_000185"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR6257441.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            79504..80376
                     /locus_tag="OO468_000186"
                     /pseudo
     CDS             79504..80376
                     /locus_tag="OO468_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(80470..81531)
                     /locus_tag="OO468_000187"
     CDS             complement(80470..81531)
                     /locus_tag="OO468_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6257442.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            81744..83207
                     /gene="bepA"
                     /locus_tag="OO468_000188"
     CDS             81744..83207
                     /gene="bepA"
                     /locus_tag="OO468_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR6257443.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            83228..83587
                     /gene="arsC"
                     /locus_tag="OO468_000189"
     CDS             83228..83587
                     /gene="arsC"
                     /locus_tag="OO468_000189"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR6257444.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(83720..84466)
                     /gene="hda"
                     /locus_tag="OO468_000190"
     CDS             complement(83720..84466)
                     /gene="hda"
                     /locus_tag="OO468_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR6257445.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(84516..85805)
                     /gene="uraA"
                     /locus_tag="OO468_000191"
     CDS             complement(84516..85805)
                     /gene="uraA"
                     /locus_tag="OO468_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR6257446.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(85891..86517)
                     /gene="upp"
                     /locus_tag="OO468_000192"
     CDS             complement(85891..86517)
                     /gene="upp"
                     /locus_tag="OO468_000192"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR6257447.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            86841..87878
                     /gene="purM"
                     /locus_tag="OO468_000193"
     CDS             86841..87878
                     /gene="purM"
                     /locus_tag="OO468_000193"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR6257448.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            87878..88516
                     /gene="purN"
                     /locus_tag="OO468_000194"
     CDS             87878..88516
                     /gene="purN"
                     /locus_tag="OO468_000194"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR6257449.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            88688..90754
                     /gene="ppk1"
                     /locus_tag="OO468_000195"
     CDS             88688..90754
                     /gene="ppk1"
                     /locus_tag="OO468_000195"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR6257450.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            90759..92300
                     /gene="ppx"
                     /locus_tag="OO468_000196"
     CDS             90759..92300
                     /gene="ppx"
                     /locus_tag="OO468_000196"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR6257451.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(92339..>93220)
                     /gene="pdeF"
                     /locus_tag="OO468_000197"
     CDS             complement(92339..>93220)
                     /gene="pdeF"
                     /locus_tag="OO468_000197"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6257452.1"
                     /translation="IYLLLGELNTVAELSIVTNAPENMQRCGAMYLQRELKDKVAMMN
                     RLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELIGPGSFLPVAHEFGLSSS
                     IDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLI
                     FEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSF
                     IRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKP
                     QPLIDTLNEIEPIRESA"
BASE COUNT        22557 a        24337 c        23497 g        22829 t
ORIGIN      
        1 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
       61 acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg cgatactcaa
      121 agattcgaac ctgcagcagg tttgagttga gcgcagcgaa acaacggagc cgctcgcggc
      181 gacggcccga agggcgagcg aagcgagtca tcctgcacga cccaccaatg caaaaaagcg
      241 ccctaaaggc gcttttttgc tatctgcgat ttgtaagatt gatttttctt ctcaagttcc
      301 ttgttcttct accgatatta cctcgtacag tgacgaggac tgaacaatgg ccactatctc
      361 ttcaacttca attccttcca tacaaacaca gagtagtaac agggcatcac aaggtagcga
      421 tgttgcttca caaatcgcgc ggataagtca gcaaatcatc aaactcactc aacaaattaa
      481 agagattgta gacactagcg gcagtgctga agacaagcaa aaacaggccg aattgattca
      541 acagcaaatc accctactgg aaacacaact ggcgcagttg cagaaacagc aggcagaaaa
      601 ggcgcaggag aaagagcagc gtttgtctct gaatgtatcc cttctaaatc ctgttgaaaa
      661 aacgactcat atcgatatct atatctgata caacggatcc ccttcccgac cagccattag
      721 cgcattgccc tggtctcgta ccacgtccca taacgcttca gcggcggtgg aaagtgaacg
      781 gttcttcctg cgcaccagca tcaactggcg ttcaaccacc ggcgtaatgc gcttaaccac
      841 cagtgggcta ccttcaggca atggtagcgc cagtgcagga agaatactga tgccgatgcc
      901 tgcggctacc atcgggaaca gcgtcgccgg atgtccaatc tcctgcacaa tattcgcctg
      961 aataccattg cgcgccagcg ctgcgtcaat cagcggtcgg ctgcctgacg cgtaatcctg
     1021 caacaccaat ttcgctccct gtaatgcctg ccagggtaca taatcttcca cggccaaagc
     1081 gctatcgcga tggcaaagca gaaagaaagg ctcggaaaga atcgcttcac attgcagatc
     1141 gcccactggg cctggatcga tgacgatgcc aaagtcgaca tctccctggc gaatactttc
     1201 catcacccac tgctgcggac gatcatgcag gacaaactga atatctggat agcggcgatg
     1261 gctttcagca atgcattgcg gaataagatg cgcggaaatg gtctggctgg cagcgacccg
     1321 aacttttccg ctcagttgtt gccccatacg cccggtatcg cgcaacgtgc tgttaagttc
     1381 atccagcagt cgctcaagac gcaaagccaa ctgctgccct gcatctgtaa gcaccacttc
     1441 acgcgtggtt ctgtccagca ggcgaacacc agtatgattt tccagctcct tcacactgtg
     1501 acttactgcc gactggctca agccgatacg ctctcctgca cgactaaaac ttttctcctg
     1561 cgctactgtg acgaaaacct ttaattgttt taaagaataa ttcatctgtt ttcttcatga
     1621 atggatgcaa taaatcaatt ttatttctca aactggaaga agcacaatag aaccatcgat
     1681 catctggagt ctttatgaaa ctttttcgta tcctcgatcc tttcacctta accctgatca
     1741 cggtggtgtt gctggcctct ttctttccgg ccagaggcga tttcgtcccc ttctttgaaa
     1801 atctgaccac cgcagctatt gccctgctgt tctttatgca cggcgcgaag ttgtcgcgtg
     1861 aggcgattat tgctggcggt ggtcactggc gactgcattt gtgggtaatg tgcagcacct
     1921 tcgtgctgtt tccgattctg ggtgtactgt ttgcctggtg gaaaccggta aatgtcgacc
     1981 cgatgctcta ctccggtttt ctctacttgt gcattctccc ggctaccgtg cagtctgcaa
     2041 tcgccttcac gtcaatggcg ggcggtaacg tcgcggcggc ggtttgttct gcgtcagcat
     2101 ccagcctgct ggggattttc ctttcaccat tgctggttgg tctggtgatg aatgttcacg
     2161 gtgcaagggg cagccttgag caggtcggta aaattatgct gcaactgctg ctgccgtttg
     2221 tgttggggca tctttcccgg ccgtggattg gtgactgggt gtcgcgcaat aaaaaatgga
     2281 ttgcgaaaac tgaccagacg tccattctgt tggtggttta tacagcgttc agcgaagccg
     2341 tcgttaatgg tatctggcat aaagttggct ggggatcatt gctgtttatc gtggtggtca
     2401 gctgcgttct tctggctatc gtgattgtag ttaacgtctt tatggcacgc cgactgagct
     2461 tcaataaggc agatgaaatt actatcgtct tttgtggttc gaaaaagagt ctggcaaatg
     2521 gcatcccgat ggcaaacatt ctgttcccca catcggtgat cggtatgatg gtgctgcccc
     2581 tgatgatttt ccatcagatc caattgatgg tctgtgcggt gctggcgcgt cgatacaaac
     2641 gccagaccga acagttacag gcgcagcagg aaagcagcgc cgataaagct taaagcggac
     2701 gcttcagggg ctggaccagt tgcgtcagcc cctcggtttt aatcagcagc gtgatttgca
     2761 ttagctcacc gagcttccct gccgggaact catctttgcg ggcaaaccac agcagatact
     2821 cctccggcag gtcaattaag cgacgccctt tgtatttgcc aaacggcatt atcgtattgg
     2881 ctatttcaat cagctgctct ttttccatct cagctaccca gcaaacgcag catttccgct
     2941 tcgtcgatga cttcaatgcc cagttcctgc gccttcgcca gtttagatcc tgcagcttca
     3001 cccgctatca ccagatcggt tttcttcgac acgctgcccg cgactttcgc gcccagttcg
     3061 accagtcgag ctttagcgtc atcacgcgac atctggctta agctgcccgt aagcaccacg
     3121 gttttaccag caaacgggct gtcaatctct tccgcgttga taacgatcgg cgcaggccag
     3181 tgaacacctt ccgccaacag ctcgctgatg acattgcggt tgctttcttc ggcaaagaag
     3241 ttgtgaacgt gggatgcaac gacaatgcca acatcaggca ccttttgcag ctcttcaatc
     3301 gaagcggctt ccagcgcttc cagcgtgccg aaatatgccg ccagacctgc tgcggtggcc
     3361 tcgccgactt cacggatgcc aagtgcatag aggaagcgag caaaggtggt ttctttcgct
     3421 ttttccagcg cgttaaccac gttttgtgcc aattttggcc ccatacgctc cagtccggtc
     3481 agtttgcctg cggtgagttt gaacagatct gccggagtat ggacatattc tttttcaacc
     3541 agctgatcga tgattttgtc gcccattccg tcaacatcca tcgcacggcg ggaaacaaag
     3601 tgtttcagcg actctttacg ctgcgcaccg caaatcaggc caccggtaca gcgggcaacc
     3661 gcttcacctt ccacacgctc aacgtcagaa ccacataccg gacaatgcgt cgggaataca
     3721 acctcacggg tatcttccgg gcgttcagaa agcacgacgt taaccacctg cgggatcacg
     3781 tcgccagcgc ggcgaatcac cactttatcg ccaatgcgta aaccaagacg ttcgatttca
     3841 tccgcattgt gtaaggttgc gttactcacc agcacgccag caacatggac aggttccaga
     3901 cgcgcaacag gcgtaatcgc gccagtacgc ccaacctgaa actcgacgtc acgcacaaag
     3961 gtcatctgct cctgcgccgg gaatttaaac gcaaccgccc agcgcggggc acgcgcgaca
     4021 aagccaagct gctcctgctg ttccagtgag ttgaccttaa tcaccacgcc gtcgatatca
     4081 aagcccagcg tcgggcggtc ttcttccact ttgtgataga acgccagcac ttcttccgcc
     4141 gattcacaaa gcgttacccg atcgctgacc ggcacccccc actttttaaa ttgcagtaaa
     4201 cggccaagat gagtatccgg cagctcgcca ccttccagaa caccaacgcc atagcagaaa
     4261 aaagtgagcg gtcgcttcgc tgtaatacgc ggatcaagct gacgcagtga accagctgcc
     4321 gcattacgtg ggttagcaaa aactttcccg cccgtgcgtc gcgcatcttc gttaatcttt
     4381 tcgaaccccg cctgcggcag gaacacttca ccacgcactt ccagacgcgc cgggatattc
     4441 tctccgtgca gcttcagcgg aatggcgcga atagtacgca cattagacgt gatatcttcc
     4501 ccggtggtgc catcgccacg ggtcgcggca ctgactaaaa cgccattttc atacagaata
     4561 ctgacggcaa ggccatccag cttcagctca cagcaccagg tgactttctc gttgtttttc
     4621 agacggtcct gcacacgttt gttgaaagca agaaagcttt cttcatcaaa aacgttatcc
     4681 agtgacagca ttggtacttc atggcgtatc tggctgaaag ctgccagcgg cgcagcacct
     4741 acacgttggg taggcgaatc aggcgtaatc agttctggat gtttggtttc cagctcgcgc
     4801 agttcgcgca tcagcctgtc gtattcagcg tcgggaattt ccggcgcatc catcacatga
     4861 taaagatatt catgatggca aagcgtcgtt cgcagttctg tcagttgttg ttcgattgat
     4921 tccatatcgc accatcaatg ctaaaaaccc ccgacaagcg ggggttcgaa gaggagttaa
     4981 tttgccttaa gtgtatcagg cgttggcgtc tttgacttcg cggatgatgt cctggtactc
     5041 gcgcaatttc tgcggagtca tcatacggcg ctgatcgtca agcacgacac cgcccacttc
     5101 atcggcaata tgctgcgcag attgcagcat cagcttgaag ttctgcagct cgtcaccgta
     5161 agacggtacc tgcataaaga tagtgacacc cggagtagtg aaatccttca tttcaggatc
     5221 aaaggttccc ggtttcacca tattcgccag gctgaataac gccgggccgc tgccatccgg
     5281 gctaagatga cgatggtaaa tattcatatc gccaaaaatg aagcccgctt gttgaatgct
     5341 gttaagaagc agttcaccgt ttagctcgct accgtgatgc gccgcgacgt tcatgataat
     5401 caccgcttct ttgcgcttcg gtttatccat aactggagcc ggttccgcta caggctcagg
     5461 ctgtggtgcc gctacgggtt ctgcaggctg gaaagcctgt tgtgccggtt gcggtgctga
     5521 atgcacaggc tgcggcgctg gcgcgacctg tggcgaaact ggctgctgca acggctgttc
     5581 aggctgcggc tgataggcag gctgctgcac cggctgcgct ggacgcggag catgttgcgg
     5641 cggtacctgc gcttcaggcg gctgctggac cggttgacgc ggctgcgcag acgcataagg
     5701 cggtaggtac tggtgttgcg gcgacggacg agcagcctca tgctcctgag cgttagccgg
     5761 ggcatgattc acgcggtgaa cacgaacctc accaacgccc tcatcatctt cgacatcctc
     5821 gtcataagaa tcgtcgtcac gttttgactt cattcgtttt aatggccgat cgcggaacat
     5881 agaagatcgt tctttacggc tggtccagaa accatgtacc agtaaagcga ttatggcgat
     5941 cgcgccaaca atgattaata tcagacgcaa atcctgcatc attatattct ctgttgttct
     6001 aacaccttgc caccacggca aacatttact cactaagagt atttgccgat tacctcaagt
     6061 gcaagtgcac tattaacttt cacagcacaa agatagatga aatcgtgctt tttgctgttt
     6121 tttcgaacat atcctaactg tccattgcgc aattacccgg taaaatacgc agaattttcc
     6181 tgggattggt caaaaggagc tcatcctgat tatggtttca tcattcacat ctgccccacg
     6241 cagcggtttt tactattttg cgcaaggctg gaagctcgtc tcgcaacctg ggattcggcg
     6301 tttcgttatt ttaccgctgc tggtcaatat tttgttgatg gggggcgcat tctggtggct
     6361 ctttacacag ctcgatgtct ggatcccgac tttcatgagt tacgttccgg actggctaca
     6421 atggctgagt tatctgttgt ggcctctggc tgtcatctct gtgctgttag tgtttggcta
     6481 tttcttctcc acgattgcta actggattgc cgctccgttt aacggtttat tggctgaaca
     6541 actggaagca cgattgactg gcgctacacc gccagatacc gggattttcg gtatcatgaa
     6601 agatgtgccg cgaatcatga aacgcgaatg gcaaaaattt gtctggtatc tgccgcgcgc
     6661 aattgtatta ctaattcttt acttcatccc tggtattggg caaaccgtcg cgccggtact
     6721 gtggttcctg tttagcgcct ggatgttagc catccagtat tgcgattacc ccttcgataa
     6781 ccacaaagtg ccgtttaaag agatgcgcac cgccctgcgc acacgcaaaa tcaccaatat
     6841 gcagtttggt gctttaacca gcctgtttac gatgatcccg ctgcttaatc tgttcatcat
     6901 gcccgttgcc gtttgtggcg cgacggcgat gtgggtcgat tgctatcgcg ataaacacgc
     6961 gatgtggcgg taacaatcta ccggttattt tgtaaaccgt ttgtgtgaaa caggggtggc
     7021 ttatgccgcc ccttattcca tcttgcatgt cattatttcc cttctgtata tagatatgct
     7081 aaatccttac ttccgcatat tctctgagcg ggtatgctac ctgttgtatc ccaatttcat
     7141 acagttaagg acaggccatg agtaagattt ttgaagataa ctcgctgact atcggtcaca
     7201 cgccgctggt tcgcctgaat cgcatcggta acggacgcat tctggcgaag gtggaatctc
     7261 gtaaccccag cttcagcgtt aagtgccgta tcggtgccaa catgatttgg gatgccgaaa
     7321 agcgcggcgt gctgaaacca ggcgttgaac tggttgaacc gaccagcggt aataccggga
     7381 ttgcactggc ctatgtagct gccgctcgcg gttacaaact caccctgacc atgccagaaa
     7441 ccatgagtat tgaacgccgc aagctgctga aagcgttagg tgcaaacctg gtgctgacgg
     7501 aaggtgctaa aggcatgaaa ggcgcaatcc aaaaagcaga agaaattgtc gccagcaatc
     7561 cagagaaata cctgctgctg caacaattca gcaatccggc aaaccctgaa attcacgaaa
     7621 aaaccaccgg tccggagatc tgggaagata ccgacggtca ggttgatgta tttattgctg
     7681 gcgttgggac tggcggtacg ctgactggcg tcagccgcta cattaaaggc accaaaggca
     7741 agaccgatct tatctctgtc gccgttgagc caaccgattc tccagttatc gcccaggtgc
     7801 tggcaggtga agagattaaa cctggcccgc ataaaattca gggtattggc gctggtttta
     7861 tcccggctaa cctcgatctc aagctggtcg ataaagtcat tggcatcacc aatgaagaag
     7921 ccatttctac cgcccgtcgt ctaatggaag aagaaggtat tcttgcaggt atctcttctg
     7981 gagcagctgt tgccgcagcg ttgaaactac aagaagatga aagctttacc aacaagaata
     8041 ttgtggttat tctaccatca tcgggtgagc gttatttaag caccgcattg tttgccgatc
     8101 tcttcactga gaaagaattg caacagtaat gccagcttgt taaaaatgcg taaaaaagca
     8161 cctttttagg tgcttttttg tggcctgctt caaactttcg cccctcctgg cattgattca
     8221 gcctgtcgga actggtattt aaccagacta attattttga tgcgcgaaat taatcgttac
     8281 aggaaaagcc aaagctgaat cgattttatg atttggttca attcttcctt tagcggcata
     8341 atgtttaatg acgtacgaaa cgtcagcggt caacacccgc cagcaatgga ctgtattgcg
     8401 ctcttcgtgc gtcgcgtctg ttaaaaactg gcgctaacaa tacaggctaa agtcgaaccg
     8461 ccaggctaga ctttagttcc acaacactaa acctataagt tggggaaata caatgttcca
     8521 gcaagaagtt accattaccg ctccgaacgg tctgcacacc cgccctgctg cccagtttgt
     8581 aaaagaagct aagggcttca cttctgaaat tactgtgact tccaacggca aaagcgccag
     8641 cgcgaaaagc ctgtttaaac tgcagactct gggcctgact caaggtaccg ttgtgactat
     8701 ctccgcagaa ggcgaagacg agcagaaagc ggttgaacat ctggttaaac tgatggcgga
     8761 actcgagtaa tttcccgggt tcttttaaaa atcagtcaca agtaaggtag ggttatgatt
     8821 tcaggcattt tagcatcccc gggtatcgct ttcggtaaag ctctgcttct gaaagaagac
     8881 gaaattgtca ttgaccggaa aaaaatttct gccgaccagg ttgatcagga agttgaacgt
     8941 tttctgagcg gtcgtgccaa ggcatcagcc cagctggaaa cgatcaaaac gaaagctggt
     9001 gaaacgttcg gtgaagaaaa agaagccatc tttgaagggc atattatgct gctcgaagat
     9061 gaggagctgg agcaggaaat catagccctg attaaagata aacacatgac agctgacgca
     9121 gctgctcatg aagttatcga aggtcaggct tctgccctgg aagagctgga tgatgaatac
     9181 ctgaaagaac gtgcggctga cgtacgtgat atcggtaagc gcctgctgcg caacatcctg
     9241 ggcctgaaga ttatcgacct gagcgccatt caggatgaag tcattctggt tgccgctgac
     9301 ctgacgccgt ccgaaaccgc acagctgaac ctgaagaagg tgctgggttt catcaccgac
     9361 gcgggtggcc gtacttccca cacctctatc atggcgcgtt ctctggaact gcctgctatc
     9421 gtgggtaccg gtagcgtcac ctctcaggtg aaaaatgacg actatctgat tctggatgcc
     9481 gtaaataatc aggtttacgt caatccaacc aacgaagtta ttgataaaat gcgcgctgtt
     9541 caggagcaag tggcttctga aaaagcagag cttgctaaac tgaaagatct gccagctatt
     9601 acgctggacg gtcaccaggt agaagtatgc gctaacattg gtacggttcg tgacgttgaa
     9661 ggtgcagagc gtaacggcgc tgaaggcgtt ggtctgtatc gtactgagtt cctgttcatg
     9721 gaccgcgacg cgctgcccac tgaagaagaa cagtttgctg cttacaaagc agtggctgaa
     9781 gcgtgtggct cgcaggcggt tatcgttcgt accatggaca tcggcggcga caaagagctg
     9841 ccatacatga acttcccgaa agaagagaac ccgttcctcg gctggcgcgc tatccgtatc
     9901 gcgatggatc gtaaagagat cctgcgcgat cagctccgcg ctatcctgcg tgcctcggct
     9961 ttcggtaaat tgcgcattat gttcccgatg atcatctctg ttgaagaagt gcgtgcactg
    10021 cgcaaagaga tcgaaatcta caaacaggaa ctgcgcgacg aaggtaaagc gtttgacgag
    10081 tcaattgaaa tcggcgtaat ggtggaaaca ccggctgccg caacaattgc acgtcattta
    10141 gccaaagaag ttgatttctt tagtatcggc accaatgatt taacgcagta cactctggca
    10201 gttgaccgtg gtaatgatat gatttcacac ctttaccagc caatgtcacc gtccgtgctg
    10261 aacttgatca agcaagttat tgatgcttct catgctgaag gcaaatggac tggcatgtgt
    10321 ggtgagcttg ctggcgatga acgtgctaca cttctgttgc tggggatggg tctggacgaa
    10381 ttctctatga gcgccatttc tatcccgcgc attaagaaga ttatccgtaa cacgaacttc
    10441 gaagatgcga aggtgttagc agagcaggct cttgctcaac cgacaacgga cgagttaatg
    10501 acgctggtta acaagttcat tgaagaaaaa acaatctgct aatccacgag atgcggccca
    10561 atttactgct taggagaaga tcatgggttt gttcgataaa ctgaaatctc tggtttccga
    10621 cgacaaaaag gataccggaa ctattgagat cattgctccg ctctctggcg agatcgtcaa
    10681 tatcgaagac gtgccggatg tcgtttttgc ggaaaaaatc gttggtgatg gtattgctat
    10741 caaaccaacg ggtaacaaaa tggtcgcgcc tgtagacggt accattggta aaatctttga
    10801 aaccaatcac gcgttctcta tcgaatctga tagcggcgtt gaactgttcg tccacttcgg
    10861 tatcgacacc gttgaactga aaggcgaagg cttcaagcgt attgctgaag aaggtcagcg
    10921 cgtgaaagtt ggcgatactg tcattgaatt tgatctgccg ctgctggaag agaaagccaa
    10981 gtctaccctg actccggttg ttatctccaa catggacgaa atcaaagaac tgatcaaact
    11041 gtccggtagc gtaaccgtgg gtgaaacccc ggttatccgc atcaagaagt aattcttgcc
    11101 gcagtgaaaa atggcgccca tcggcgccat tttttatgct tccgccagcg gcggcaaaat
    11161 caattcatcg ctctcatgct gctgggtgta gcgcatcact tccagtacgc gcaaccccgc
    11221 acggtgcacc gcatcgttta acgccttccc tttcagcaaa ccactgataa gctgagcaca
    11281 aaacaggtcg ccagtccctt tcaggtcggt ttttacccgt gaatgggaaa tgacattcac
    11341 gctgtcggca ctgaccacca ccacctgcat ctcctgattt tcttcattac cggaggcgct
    11401 ggtaatcacc acccatttta atgtgtctga aagcagactt tttgcggcag caatggcact
    11461 gtcgagatcg cggcaatttt taccggtcag gatttccagc tcaaagatat tgggggtaat
    11521 tccctgcgcc agcggcagta aatattgtcg atacgcctcg ggaaggtcag gtttgacata
    11581 aattccgcta tcaatatcgc caatcaccgg atcgaccatg atcaataggt caggatggtc
    11641 tttgcgtagc gcagtcagcc actcggcaag gattttgatt tgcgatgccg ttcccatata
    11701 gcccgtggtt acagcacgaa gttggcgcag cgcatcacgc tcctgaagcg cacgcaaata
    11761 gccgctaaac cattcgtccg gaatcgcacc accgtagaaa gtgtcataat gcggcgtatt
    11821 gctcagcaat accgtcggca cggcaaagac attcaagccg ttctgtttta tagcaggcac
    11881 cgcaatgctg ttgcctacgc ttccgtacac tacctgcgac tgcacggcga cgatatccgc
    11941 ctgcagcgcc ctactcttat cgttaaacaa caacaaacta ctcatttaat tttttctcct
    12001 tgccgatgat cctcatcgta ttccaaccga aactttacct gattctggca gtcaaatcgg
    12061 ctattacaaa acaaagataa ggtaattcag tgaagaaaat catttgtctg gccattacgc
    12121 tgctaatgac actccccgct tacgcgaagt taactgttca tgaagaagcc cgcatcaacg
    12181 ccatgctgga gggattagca cagaaaaagg atttgatatt tgtgcgcaac ggtgatgaac
    12241 atacctgcga tgaagcggtt tcccacctgc gtctgaagct cggcaatacc cgtaaccgca
    12301 ttgacactgc cgagcagttt attgataagg tagcttcgtc gtcatcgatt accgggaagc
    12361 cgtatatcgt gaagatcccc ggtaagagcg atgagaacgc acagcctttt ttacatgcgt
    12421 taattgcgca gacggataaa acggtgcctg cggaaggaaa ttaatccgct ttggtaaggc
    12481 atttacagga ggtaacatga aaaaatgctt tatttatcac gatgaaaaat cgaataaatt
    12541 ttggtggata gattacgaag gggatagttt agctgtcaac tatggcaagg taggtagtat
    12601 tggtaaattc catacaaaag aattcgataa tgaagaacag tgtctgaaag aagccagtaa
    12661 attgattgcc gcaaaaatga aaaaaggata tcaagaaact ccagacttta acttcatgga
    12721 tctctactat tttgatgatg aagagattgg gttacatctt aaaacgtcac acccaaactt
    12781 ccagtgccat tttactgatc cactttatat gtgttgctgg gatgaagaat ctccttttgg
    12841 tagcgatgaa ggtgctgatg ctctaaacga acttgaaaat agcctccgtt ctggactgtg
    12901 ctgatttccc tcaaatgtta attgaaacta tgtggggtat gaaatacatc gctatggaca
    12961 gtattcttga agaggatgtg cgtgcgcaat tactagccga tgaaatgagc tctatccaga
    13021 gcaatatgat tacctacgcg actgcattcg gtcagatcaa agtcatgggt aaaatctccc
    13081 ataaacttaa aaagatggga ctcaatgcac tagcgcgtca tcagcttacc gcaaaaattc
    13141 ttcaatgggg tgacggtcag gactcaccaa tacttcaaaa aatgattgat gaccttactg
    13201 cttttccaca cgaaaattaa aaactgcatt tgtcggcagc aacaactgcc gacaaatcag
    13261 attattgaca aagtgcactt tgtttatgcc ggatgcggcg taaacgcctg atccggcata
    13321 aaaatattgc taattcaata tattgcagaa acattgtagg cctgataagc ggagcgcatc
    13381 aggcagtttt gcgtttgtca tcagtctcca atgctattaa tccttaaatc cctgccccct
    13441 gactaaaatg ctcttcccca aacactccgg tggagaggta gcgatcgcca cgatcgcaga
    13501 tgatcgctac caccaccgca ccagggttag cttttgccac ccgcagtgct ccagcaaccg
    13561 cgccgcctga gctgacgcca cagaatattc cttcccgcac cgccagttcg cgcatggtgt
    13621 tttccgcatc gcgctgatga atatccagca cctcatccac cagcgcagcg ttgaaaatcc
    13681 ccggcagata ttccgcaggc cagcggcgaa tgcccggaat actgctgcct tcttccggtt
    13741 gcaggccaac aatggtcacc ggtttggatt gttcgcgcat aaagcatgaa acgccggtga
    13801 tagtgccggt cgtccccatg ctggagacaa aatgagtgat gcgcccgccg gtttgctgcc
    13861 agatttccgg cccggtggtg gtgtaatgcg cataagggtt atcgggatta ttgaactgat
    13921 cgagcagctt tccttcgcca cgattcgcca tctccagcgc cagatcgcgc gcaccttcca
    13981 tgccctgctc tttggtgaca agaatcagtt ccgcaccata agcacgcatc gccgcacggc
    14041 gttcctggct catgttgtcg ggcatcagca atttcatgcg atagcctttc agcgcggcaa
    14101 tcattgccag cgcaatgccg gtgttaccac tggtggcttc gattaagaca tcacccggtt
    14161 taatttcccc gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga tctttcaccg
    14221 aacctgccgg gttattgcct tccagtttta accacacttc actgccgtta tccggcccca
    14281 ttcgctgcaa cttcaccaga ggcgtattgc ctattgtttg ttctaatgta ctcacaatct
    14341 ctatccatac gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggc ctacaggtta
    14401 caaaccttgc cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt taacattcac
    14461 tcaacctatc aggcgctttg tgcgagagca agttcctcat cgcgggtttc aatacgctcg
    14521 tcgccgttat acagccgcgc atgttgcaga ccaacgaata aacgctcgcc atgctgcggg
    14581 gcatcgtcgc catgcatcac gaccgtcagc ggttcgttgt accaccccag cggctgcacc
    14641 actaattggg tgtagtgacc tttcgggctg gcttccagta cctgtaccgg cagcggcgaa
    14701 tcgagactgg tacggcggct gatatccact tcccacgggc gcaggaagag atccaccggc
    14761 ccctgatacg caggtgtgta acccagcggc cagcgatgcg cgccaacatg gaactgcccg
    14821 ccgcgaatgg ttccctgcag gcggttcact tcgcccataa attcgagtac aaaacgggtc
    14881 gctggttcgc gccatacctg attcggcgca tcagcctgtt caatattccc ctgactcatc
    14941 accactacac gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac aaacacgctg
    15001 gtgaatttca gctcttcatg cagttgacgc agccagcgac gcagctcttt acgcacctgc
    15061 gcatccagcg cgccgaacgg ttcatcaagc agcagaattt gcggttctac agccagcgcg
    15121 cgcgccagcg ccacacgctg tttctggccg ccggaaagct gcgccggata acgatccgcc
    15181 agatgcgcaa gctggaccat ttccagcaat tttgtcactt tcgctttgat ggctgcggca
    15241 ttcgggcgct cgcgacgcgg cagcaccgtc aggccaaaag tgatattgtc gaataccgtc
    15301 atatggcgga acagcgcgta atgctggaac acgaaaccga ctttacgatc gcgcgcgtgc
    15361 aggcgactca cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg ctccagcccg
    15421 gcgataatcc gcagcagcgt ggttttcccg gaaccggacg gccccagcaa cgcgaccatc
    15481 tgaccagaag gaatatccag tgagatatcg ttcagcacct gggtgcgacc aaaagacttc
    15541 ttgatattgg caatctcaat gctcatgatg ctcctcctgc tgcgcgcgtt tttcctgatt
    15601 ctccaggcgc cactgcaaca tactctttaa aaacagggtg ataatcgcca tcaacgtcaa
    15661 cagcgcggcg gcggtaaagg agccgacggt gttgtagtcc tgctccaaca attcaatctg
    15721 taacggcagc gaaagtgttt cgccgcgaat cgagccggaa accaccgaca ctgcaccaaa
    15781 ctcgccaatc gcgcgggcgt tggtcagcac cacgccgtaa agcagcgccc agcgaatatt
    15841 cggcaatgtg acgcgacgga acatctgcca gccggacgcg ccaagcaaaa tcgccgcttc
    15901 gtcttcctgg ctgccctggc ttaacatcac cggcaccagc tcgcgcacca caaacggaca
    15961 cgtcacaaag atggtgacca gcaccattcc gggccaggag aacataattt gcagattatg
    16021 ttcatccagc cagccgccga gcgggccgtt ggagccgtag aacagcagat acaccagacc
    16081 ggcaaccacc ggcgatacag caaacggaat gtccagtagc gtcagcagta actggcgtcc
    16141 cgggaagtta aagcgcgtca ccagccaggc cagcagaatg ccgaacacca ggtttaccgg
    16201 tacggcaatc agcgcgatca tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc
    16261 cagattctgt aaaaccggca tcagcccctt gctgaatgcc tgcacgaaga tgtaaatcat
    16321 cggcaccagc aggatgaacg ccgaaaccag catcccgatg ccaatcagaa accatttgcc
    16381 ccagttaatc gggcgcgcgt cataacgctt caattgggta acttccgcca ttaatgacct
    16441 accacacgcc gaccaaagcg actttgcaga gtgttaattg agaacagcag cagcagagat
    16501 gccgcgagga tcaccgaagc aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc
    16561 acaaaaatca tcagcgacgt cacttccgtc ttccacgcga tatttccggc gataaaaatc
    16621 accgcgccaa attcaccaag actacgggta aacgacagcg ccacgcccgc caccagcgcc
    16681 ggagaaagct ccggcagcac cactttgcag aaactctgcc agcgcgttgc accaagcgtt
    16741 tccgccgctt cttcatattc cgggcctaac tcttccagca ccggctgcac ggtacgcacc
    16801 acaaacggaa tgctggtaaa ggccatcgcc accgcaatac caagccaggt ataggtgact
    16861 ttgatatcaa acttcgccag ccattcaccg taaaaaccgt ttacggaaaa gagcgaggcc
    16921 agcgttaaac cggcgactgc cgttggcagc gcaaagggta aatccatcag cgcatcaagc
    16981 agcgtgcggc ccggaaagcg ataacgtgtt aaaatccacg ccatcagcag accgaaaacg
    17041 ccgttaaaaa tcgatgccac aaacgccgac agcagcgtca ctttgtaggc cgcgaccacc
    17101 tgcgggttgg tgatcacctc ccagtactgc gcccagctca tctcagaaag ctgcatcacc
    17161 agcgcagaga gcggcagcag caatatcagg cacacaaaca gcagactggt gccaaggctt
    17221 aaagtaaagc ccggcagcac gcgtcttgaa gaaactgcaa acattactta cgccccgccg
    17281 ctaacagctt gtctaactcg ccgccgctgg tgaagtgggt tttcatcact tccggccatg
    17341 agccaaattt gtcttccacg cggaacagtt cggtctgcgg gaatttatct ttcagtttgt
    17401 ccatgacttc cgggttattc acacggtagt aatagtcggt gatgatggtc tgcgcctgtg
    17461 ggctgtagag ccagttcagg taggcttttg cggctttttc cgtaccgttg gcctgcacat
    17521 ttttatcgac ccacgccacc gggaattccg ccagaatatt ggttttcggg atcaccactt
    17581 caaagccctg tgcttcatac tgtttacgga tgttgttcac ttccgattca aagctaatca
    17641 gcacatcgcc caagccacgc tcggcaaaag tggtggttgc gccacgaccg ccggtatcga
    17701 acacttcaac gtttttcagg aactgggtca taaactgttc ggttttggct ttatcgccac
    17761 cgtcagcttt atccgctgcg ccccatgccg ccagataggt ataacgcgcg ttacccgacg
    17821 ttttcgggtt cgggaaaatc agcttcacgt cggagcgcac caggtcgttc cagtcgtgga
    17881 tattcttcgg gttgccctta cgcaccagga agcccatggt ggagtagaac ggcgagctat
    17941 tattcggcag gcgcgactgc cagtcagccg ggatcagctt gcctttgtcg tgcaggattt
    18001 gtacgtcggt cacctggtta taagtgacaa cgtctgcttt taagccctgc aaaatcgcca
    18061 gcgcctgttt tgatgacccg gcatgagatt gttttatcgt cagtttgtcg ccgccgttat
    18121 cttttgccca ttgctgttca aacggcggat tcagggcggc aaacagctcg cgggagacgt
    18181 cataagaact gttcagcagt tccgttgcct gtacatggcc cgccagcagc agagaagcga
    18241 ccagcgcgag tgagttcttt ttcagtaagt taacggccat tgcgcaccct tataaattta
    18301 atgactttct aatagccatc atatttataa cggacgtgaa aggagtaacg gttttatata
    18361 ccgtttggtg atttggaagt taaaaaggga ataagaactt ggttcaacag gcgtaattgt
    18421 tgcaggcagt ttggacacgg acagcgcgga gaaaccggag cgtacacata gtacgtgagg
    18481 atttcgagca ctgcccgggg ccaaaatgac aaatgaaata gcctggtgaa cttagttcaa
    18541 gacgaaatcc tccccacaca tgcagggagg aaacagaggt gaatcagata ccgacgctaa
    18601 ccgtctccgg cagtgtgctg ccgccatcaa tcacattctg tgtaccggtt aaatagctgg
    18661 attcatccga tgcgaggaag gccgccagtt cgccgacttc cagcggatcg gcgaggcgac
    18721 acagcgggat tgcttttgcc atttcagtca gcaccgattc tggatcttcc gggttcgact
    18781 ggcgggcaat gctttccgcc attggcgtgc ggacgtatcc cgggcagatg gcgttaacgc
    18841 gaataccgga ttgcgcgtac tccaccgcca gcgatttagt caggccaaca atcgccgctt
    18901 tcgttaaggc atacgccgtt tcgccaggat cggccaccat atcaccagtg actgaagaca
    18961 tcatcacaat gcgaccatct ttgcgcgcaa tcatctccgg cagcaccgcc ttcgtgacgt
    19021 tccatacgcc tttaatattg atatcaatat ggaaatcgcg atcttcatcg ctcatatcga
    19081 ggaaactgcc cagacgacaa acgcctgcgt tattcaccag gatatcaatg cgcccttctt
    19141 tttccttcgc gcgtttgata gctgcggcta ccgacgccgg gtcacgcaca tcggcgacaa
    19201 ccgccgtaca gcgatgacca cgaccacaca gttcgtccgc tagcttttcg atctcagggg
    19261 agatatccag caagattagg ttcgcgccat gacgtgcaaa agttctggca attccttcgc
    19321 caattccctg caatgcgccc gtaatcagtg ctgtcttgcc cgtgagttta cccattttaa
    19381 tgctctcctt gttaatacag agcgttacac tccattccct ctgaaaatac aggataaaca
    19441 aaatattgct gcggatcagc tcactaattc aggaaaatct caatccagtc gctgctttat
    19501 gcatcatttc aggcgttgtg ttagctcact gctgcgctga atcattttca atgacgccac
    19561 atcattgatg ttgcacaaga ccaacaaaca gtaaatcggt aacagaattt tgcgccgtgc
    19621 gggtagacat cgacgagcta cgccattcgg tttcaccgga aacggtatcg agcgtgaaat
    19681 gcgccagccg ccgcagcgga gagtcagcca gagaagtaat ggcaatcacc gttgccccct
    19741 gctttcgtgc cgcttctgcg cacaagacaa tctctttttt actgccgctg taagagatag
    19801 cgatttgtac atcgcctttt ttcaacgcct gggaaacggt ggcttgtacg tgcgtgtcgg
    19861 cttcgcaggc cacgcgatag ccaattttca tcagtttgaa cgacagatcg cgccccacca
    19921 gcgccgatcc gcccaggccc gttatctgga taaacggtgc tttactgatg acctcaatga
    19981 ttttttgcag ccgcgcgtag tcgaacaacg cacaggtttg ctccagcgcc agctcttttt
    20041 cgcgattcag tttgcgggca atcacttcca gcgaatcatc actggtaatc gaactgtgca
    20101 ggtgcagcgc cgtggcattc gttttctccc ggctggcact gtattcgcca attaacgcca
    20161 tacgcaattc agtaaatcct tgcgcgccga gcttctgggc aaatttcaca atgctcgact
    20221 ggctgatacc gagctgtttc gccatctgcc gtgaagagac ggatttcagc tcgctcactc
    20281 tggcccgcag aaagtcagca attttttgct cgttcccggt aaattcactt tccgcattgc
    20341 gtattttcgt caggtacaac atcttccact ctcctgggct ggcagcaaaa attccgctta
    20401 agttcttatg acgctcttca cactctgcga gtgtagactc aatgattcct ttaagtctgt
    20461 caattcggga tatatgattc catacatctg caattatttg atcgtaaata gtaaggtcac
    20521 caccgatgca actagaaaag atgattactg aaggctcgaa caccgcctcg gctgaaattg
    20581 accgcgtatc gacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccgc
    20641 ctgccgttga tcgcgtcctg ccggatatcg ccgcggcgat cgatgttatc cacgcccagg
    20701 tcagcggcgg cggtcgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc
    20761 tggatgccag cgaatgtccg cccacctacg gcgtgaaacc gggtctggtg gttggtttga
    20821 ttgctggagg cgaatatgcc attcagcacg cggtggaagg agctgaagat agccgggaag
    20881 gtggtgttaa cgatctgaaa aatattaatt taacggcaca ggatgtggtg gttggcattg
    20941 ctgccagcgg tcgcacgccg tatgtgattg ccggactgga atacgcacgc cagctcggct
    21001 gccgcacagt gggaatttcc tgtaatccgg ggagcgccgt ttcaaccacc gctgagtttg
    21061 ccattacacc gattgtaggt gccgaagttg ttaccggttc ttcgcggatg aaagcaggta
    21121 cagcgcagaa actggtgctc aatatgcttt ccaccgggct gatgattaaa tccggcaaag
    21181 tgttcggcaa cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg
    21241 tcaatatcgt taaaaacgcc accggatgta atgccgaaca agcggaagcg gcgttaattg
    21301 cctgcgagcg caactgtaaa acggccattg tgatggtgct gaaaaatctc gatgccgcag
    21361 aagctaaaaa acgcctggat caacacggcg gctttattcg tcaggtttta gacaaggaat
    21421 aacccatggc caaagagatc agcagtgaac ttctgaacac cattcttacc cgtgtcggcg
    21481 gaccgggaaa tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg
    21541 acagttcact ggttgatccc aatatcaaaa cgctggaagg cgtgaagggc gtcattttga
    21601 ccagcgacca ggtgcaggtc gtttttgggc ctggtaaagc gcatcgtgcc gcaaaagcga
    21661 tgagcgagct gctgggcgat gcaccggtac aggatgccgc agaaatcgcc gcccagaaca
    21721 aacgtcagtt aaaagccaaa caaacctccg gcgtgcaaca atttctcgcc aaattcgcca
    21781 ccatcttcac gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga
    21841 cgttaattgc cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgacgcgc
    21901 tgaattttat gaaggtgttc agcaaaggtt tgttcacctt cctagtgatt ctggtgggct
    21961 ataacgccgc ccaggcattt ggcggcacgg gcgtcaatgg cgcaattatt gccgcactct
    22021 ttttgctcgg ttataacccc gctgcaacca ccggttacta cgccggtttt cacgatttct
    22081 ttggtctgcc catcgatccg cgcggcaata ttatcggcgt gctgattgcc gcgtgggcct
    22141 gcgcccgcat tgaaggcatg gtgcgccgct ttatgccgga cgatctcgac atgctgctga
    22201 cctcgttaat caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg
    22261 gtggctggtt attcgaaggt atgtcatggc tgtttatgca cctgaacagt aatccgttcg
    22321 gttgtgcggt tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct
    22381 ttattcctgt ttacctcgcg ttaatggaca gccagggatt caacagctta tttcccatcc
    22441 tttcaatggc aggcgcgggc caggtgggcg cggcgctggc actctactgg cgggcgcaac
    22501 cgcacagtgc gctacgcagt caggtacgcg gggcgattat tcccggcctg ctgggcgttg
    22561 gtgaaccgct gatttacggt gtcaccctgc cccgcatgaa gccgtttgtt accgcctgtt
    22621 taggcggcgc ggcgggcggt ttgtttatcg gattgatagc ctggtggggt ctgccgatgg
    22681 gcttaaacag cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcac
    22741 aaggcatcct tccggcaatg gcggtttatg ctggcgggat tctggtggca tgggtttgcg
    22801 ggtttatttt caccacgctc tttggctgcc gtaacgtcaa tctggactga tattatgaaa
    22861 cggacaatgc tctatctttc tctgctggct gttagttgta gcgtcagcgc cgcaaaatac
    22921 cctgttctga cagaaggtag ttgatcaagt atatgcggcg ttaaagcagt aataaaaagg
    22981 ccagtcagta atgttactgc ctggccttta atcaatgaat cagaaacgct tacagcgcca
    23041 tcaacttgtc cagcgacggt gcgaaataat agccgccggt taccggtttg gtgaaacgca
    23101 acatcgcatc acgtttgcca tcggtatcgc caaacatgct cagcagttgc tgctcaatgt
    23161 tatgcagacg cgcgcagtag gcgcagaagt acagaccgtg agtgccactg gcggtgccgt
    23221 acggcaggct ctggcgaaca atcttcagcc ctttgccatc ttctttcaga tcaacgcggg
    23281 tgaggtgaga ggtttccgga cgttcgtcgc cgtcaatctc ttcgttggcc tctttggtgc
    23341 gcccgatcat catctcctga tcgtgaacgc tcatccggtt gagctgcttc aggttgtgct
    23401 cccaacgctg cacaaacaca tagctgccgc cggaatccac gccgtctttg ataaccgcca
    23461 cttcgcggcg cgtctcttca cccgccgggt tttctgtacc gtcaacaaag ccgctcaggt
    23521 cacgctcttc cacccaacgg aagccgtgga tctcttcttt cacttcaatg cagtcaccaa
    23581 aggcttccat cgccgcctga gcgacagaga agtttacgtc gtgacgcaga gaaagaatgt
    23641 ggatcaacac atcgaactgg gtcgtcggcg caaggccttt accgtagccc ggaaaatctt
    23701 tcagctcttc tgccccaacg ccgccgctca gagcgcgcca ggtgttgtta ccaaaggcaa
    23761 ccaccgcacc aagatgcgcg tccgggaatt tcgcttcaaa agttgccagt ttgtcggcaa
    23821 atgttttact ggccgcacgc agggcgtcaa cttccccttt cacgttggct tcgatccaaa
    23881 tcgccgcgcg gcaatgttct ggcaaaatgc cactctgaac ctgagacatt gttcctcctg
    23941 aaaataagaa tgccacgcaa tccgtggcat tgttgagcgc tattttacgt gttttttcgc
    24001 gacggcggtt tgttcagacg caaattaacg ccgccagata attttactga ctttccagtt
    24061 tttcagggta tcgtccgaag gcattaaccc ttcaggacca cgccattccc cgctgaactg
    24121 gtagctaata tgttgactgc cttcggcttt acattcgacg gcacgattat catcaccatc
    24181 agctttttgg caattgccaa atgctttgct gtaaaggtcg ctaaacggtg taccgatttt
    24241 aacaccggtg tcagcaggaa tatcgctatc cagcacgtca atgcgactga tcgtgccttg
    24301 atcgccgtta atcaccatcg ccacgttgtc gcctttcatc acttcaaaaa agcgcaccac
    24361 gttgccgttc gtggttttca ttccgctgcg caggcgataa tcgccatcaa gcgcatcagc
    24421 aatggcttgt tcttgcagtg gtgtggacgc cgttaattca cccacgccct gctcgctcac
    24481 tttggtggac gatccaaacc agttccacgg gttagcggca gaccagttaa ctgacgacat
    24541 cgtggaacag ccggtcagca tcaacggcat agcgcataac attaaacgca gcgatttcat
    24601 gtcacttcct ttggttattc aataacgttg cttggagtgc aaattcacca aaaagtgccg
    24661 ttattctttt tcttcattaa aacaagcgcg taaacgtcga ttggtcagca gccagattaa
    24721 cgccacaata tccgccacta ccagcgccag accgataccg ctaacggatt caccgttcag
    24781 ccacagccac ggttgccagc aaagtaaaac cacctgcgcc aacagcaaca gaaaatagag
    24841 cacatgccag gtgcgaggaa acgaagcccg ccgaccgctc aacaaaaacg ccagcaccgc
    24901 cggaataccg ggaatcagcc ccagccagaa attatcgtga tcgggataaa acagatttag
    24961 cagcgcagta ccctgctcac gcgacgcacc ggcaatgaca aacagcaccc aggttcgcgc
    25021 ctgaagcaat agcacaagcc agaagagcaa gggtaaacgc aggcgtccgt gcgcatcata
    25081 atggacagga tggaactcag tactcttcat cttcaatcaa acgcttaccc agactcagca
    25141 cgtcggcgtg ttcatatccc aggcgttcat acattccgag caccatgtcg ttatcttccg
    25201 gcacgttgat ctgaattttc gggcagccgc gagcaatcag ctttttctcc agccggttaa
    25261 gcaacgcatt ggcaattcca cgcccacgaa actctggatg cacgccaaga taataagcag
    25321 acccgcgatg cccgtcataa ccgcccatca ccgttccgac cacttcaccg tttacctcag
    25381 cgaccaaaaa caaactgacg tcatggttca tcttacgctc gatgtccatt tccggatcgt
    25441 tccacggacg cagcaagtcg caacgctccc aaagggtgat gacctcttcg aaatcttcct
    25501 ggcgaaatac gcgtatctcc atggtattcg ttaccttttt gcgggttaaa aggctgatta
    25561 tggcgtgaac ggtcgaatta gccaatatct gacgaaaatc ggttgaaaaa gtggcataat
    25621 ggggagttgt caactattga aatgaaaagt aaaacaattc tcaacagcaa accgtcgtaa
    25681 cggattacgc gatacgatat aacatctgga actttattat tacaactcag gccgtatgag
    25741 cacttttaaa ccactaaaaa cactcacttc gcgccgccag gtgctgaaag ccggtttagc
    25801 tgccctgacg ttgtcaggaa tgtcgcaagc cattgccaaa gacgaacctt taaaaaccag
    25861 caacggacac agcaagccga aagccaaaaa atctggcggc aaacgtgtcg ttgttctcga
    25921 tccaggccac ggcggaattg ataccggagc gatcggacgc aacggttcga aagaaaaaca
    25981 tgtggtgctg gcgattgcta aaaacgtccg ttccattttg cgtaatcatg ggattgatgc
    26041 gcgtttaacg cgttctggcg atacgtttat cccactttac gatcgcgttg aaatcgccca
    26101 taaacatggc gcagatctgt ttatgtcaat tcatgccgat ggctttacca acccgaaagc
    26161 tgccggtgct tcggtatttg ccctctctaa ccgtggggca agtagcgcaa tggcgaaata
    26221 cctgtctgaa cgcgaaaacc gcgccgatga agttgccggt aaaaaggcga ctgacaagga
    26281 tcacctattg cagcaagtac tgtttgatct ggtgcaaacc gatactatta aaaacagcct
    26341 gacgcttggc tcgcatattc tgaaaaagat taagccggtg cataaactgc acagccgcaa
    26401 caccgaacaa gccgcgtttg tggtgttgaa atcgccgtcg gttccttcgg tgctggtgga
    26461 aacctcgttt atcaccaacc cggaagaaga gcggctgtta ggcacggcgg cgtttcgtca
    26521 gaaaatcgcc acagcgattg ctgaaggcgt gatcagttat ttccactggt tcgacaacca
    26581 gaaagcacat tcgaaaaagc gataagttat gaaacccgac gcacaccagg ttaaacagtt
    26641 tctgctcaac cttcaggata cgatttgtca gcagctgacc gccgtcgatg gcgcagaatt
    26701 tgtcgaagat agttggcagc gcgaagctgg cggcggcggg cgcagtcggg tgttgcgtaa
    26761 tggtggtgtt ttcgaacagg cgggcgtcaa cttttctcac gtccacggtg aggcgatgcc
    26821 tgcttccgcc accgctcatc gcccggaact tgccgggcgc agtttcgagg cgatgggcgt
    26881 ttcactggta gtgcatccgc ataacccgta tgttcccacc agccacgcga atgtgcgttt
    26941 ttttattgcc gaaaaaccgg gtgcagagcc cgtttggtgg tttggcggcg gcttcgattt
    27001 aacccctttc tatggttttg aagaagacgc cattcactgg caccgcaccg cccgtgacct
    27061 gtgccagcca tttggtgaag acgtttatcc ccgttacaaa aagtggtgcg acgattactt
    27121 ctacctcaaa catcgcaacg aacagcgcgg cattggcggg ctgttctttg atgatctgaa
    27181 cacgccagat ttcgaccact gttttgcctt tatgcaggcg gtaggcaaag gctacaccga
    27241 cgcttattta ccaattgtag agcgacgtaa agcgatggcc tacggcgagc gcgagcgcaa
    27301 ttttcagctc taccgtcgcg gtcgttatgt cgagttcaat ctggtctggg atcgcggcac
    27361 gctgtttggc ctgcaaactg gcgggcgcac cgagtcgatc ctgatgtcaa tgccgccact
    27421 ggtacgctgg gagtatgatt atcagccaaa agatggcagc ccagaagcgg cgttaagtga
    27481 gtttattaag gtcagggatt gggtgtaact ccctcacccc cattcccgca tccgctgatg
    27541 caacgtcaac gacggcttct cggcaaacag ctgctggtaa tccgtggcaa attgccccag
    27601 atgccagaat ccccactgca tggcggcgtc ttttaccgtt gtgctttgcg accacgggct
    27661 tatcagttcg cggcgtacga cgttcaggcg aatgcgtttc agccacgcgt ttgggccaat
    27721 gcctaaaata gcgtgaaacg cgttttgtag cgtgcggcga ctgacatgca gttgattaca
    27781 caaatccagc accgtcaccg gttcggacat gttttccagc acatattcac gggcgcggga
    27841 aagcaaccga cggtaactct gatgactgat gctttccgcc gtcaccattg gttgcgcttc
    27901 ttccagcatc gcccccatcg ccattagcaa attatccccc agcacttttc gcactgcagg
    27961 ctgatggaga ttttccgggt tctcgcaaaa cgtcgccagc gcctgttgga caaagcccca
    28021 cagcgcggct ttatgctgct ctttcacttc cagcgccgac tggctacgca gcatatgtaa
    28081 tacccgatcc ggattgtgca aaaagttagc ctgacaggtg atgacatctt cagacagcac
    28141 tacgcccagg atcgtataat cgtccggcgt acttaattca aactcggttc caccggggcg
    28201 cgtcgctatt tctgcgcttc ccagacattg cgaaccgata aatccctgct caccgcgcgt
    28261 cgccggaatg ccaaaccaga acgagttcgg ccagaccagg cacgactgac gcagcgccag
    28321 accggtgtat tcacgaaaaa cctgaatatc atcgagtaga atttccgtaa attcaccatg
    28381 aaacttgccc ggatgcagct gatcgtaaat ctgctgctag gcggtaatcg tcaaagcatg
    28441 ttcatagaca tccgttgtct gtcgttgatg aacattatcc acttcgacct tcggcgtgag
    28501 cttcaggttt tcgggtaagg gttcatgata aagatggtgc aaattggctg tacgggtctt
    28561 tttcatgatg ttaatgccgg gtgttgtagg acacccggca cctccgacag gttaatgggg
    28621 cttgagacga taacgactac tgcgtttacg taacgtcccg gcagaaaaga gctgttcgag
    28681 cgcatttctg gctttttcca gcggccagcc aaagtgggcg gccacttctc ctgccgtcat
    28741 tccctgacat actgatgtta acagcgccaa caactcgtca gcagattccg cgacgataac
    28801 tggcgcgtcg ggttccttta cgggttgctt cggctggcgg ttaaagccag taaccagcca
    28861 ctgggtgtca tcgtctggcc gaccgatctc cttacggctg atgacacgcc cggtacgcat
    28921 cgcggcagca caacctgcgt ccagcgccgc acgacacgcc gccagatcgc cttccaccac
    28981 cagcgttaag cgaccagggt caagcacttc gtgactgagc agacgaacgt tagctgcttt
    29041 gagcatggca tccgctgcat ctatcgcggc gaccatgccg tccacttcca gcaatcccag
    29101 tgcattgatc atcggcaacc tccgttacgc acgctggatt ggattacgcg cgatttccag
    29161 cactgcatcg gtaaaggcgt tacaggctgc tttacacgcg gcctggctac cggttaaaaa
    29221 tgctgccgag tagttggttt cagacggtgg cgggacgtag gtcaccagct gtacgtcggc
    29281 agatttcaac gcggcatcaa tgccgtaggt cgcttccagc ggcggtgcca ccagatacgc
    29341 catcggatcg ccaagcgtga tcccggcggt tgatgaaaga taagaaccgg tacgcgaaac
    29401 cacatgcgcc aggaaggcgg tgttttctgc gtcattggcc cactggaaag ctgcgccatt
    29461 ttcaatatgt gcaaccatcg catccagacc ggcacgcact tccgccgggt ttggcccacc
    29521 aagcataatc agcacttcac cggcagtcgg tgacgggccg tgtgccgctc ccgcatacag
    29581 cgagcgacca tataccactt cgaccatcgc ttgtttggtc gcttcgtcag ccgcaatgta
    29641 ggttacgtcg tcagaatcgg cagaaatcag cccgaggcta cgaatatgcg gcggcaattt
    29701 tagttcacgc gcaaattcag cgttaacgga ggcaatcacc cgcatggcgg ttaccgacgg
    29761 tcgaatcaaa tctaaagctg gcatgatgcc tccttatcgg gtcatgttga tgccggatgc
    29821 tttctgctcc agcatacgtt tcgccaaatc cacaatgacg gcacagcagc acgaaagaga
    29881 agcaaaagtc agctacctgg tacactaggc agcgggtcag caaagggggg caaatgcccc
    29941 ttttttcatg tcagataatc agatcattac ctcaataaac taataatcac atcgcggtta
    30001 caaccagttt gatagtgata gctcaaaccc agacgtggcg ctggctgcaa gcaaaataag
    30061 ccgcaaaaat gcgaccccat gtttactagc cgcaaaaatg cggtcctatt tgccagaacc
    30121 ttttattatt caattggtta gtgcaagaaa aagcgtgatt actgttcaag aagtatccag
    30181 aaaggtcatg attttgacgc tgaaaaaaaa taggtgggca tttttgccca ctcactagcc
    30241 gcaaatttgc ccactcacta gccgcatttt tgcggcccaa agtaataaat acagagaaat
    30301 aataaataca gatcagcgcc aagatttttc gtcttttcgg cggaatcatg agactagtgc
    30361 ttacgtcctg ataacctcca gagcagacct taacttctgt gcagcaggac tacgctcatt
    30421 gccgctcggc taggtcaaat cctcacgata aagctgttcg gattgtgacc ataatgttaa
    30481 aggactcttt aaatcattcc ttgacgaatt aactagctgg cttaagcgga atagcatgca
    30541 ggataaaaac aaaaccagct tagcagtggg gagagcttcg cttcgctcgt ctcctggggc
    30601 caatgctcaa tgaaacgacg taaggcacgg agcgctcacc ataggtagac acataaacat
    30661 ctgagagcgc accttctatc gcgctacgcg ctcaataaaa accaacgaca ccgccacact
    30721 gcaagaccaa tggtttttat cgcgtataca gaccagacag gaaacagcag caaagcagcg
    30781 tatagccagg tgcagacagc acactcacac gacgcacagc aggcttaagt gatcactcag
    30841 gaaactttat ccccgccgcc gatcaccgcg aactatcatg accgcgccaa attcgaaagg
    30901 aatggcccat agaacgcgat gaacacacat aagcgctaca cttacttaac gtcgccacag
    30961 agcgattgag agcgcttaaa atcgatgcga tttcattaaa tttttctcac tcgcctgaat
    31021 ggtggcgatc aggactgatc gcaacattgc cccgcctcga tattgagcgc ataatagata
    31081 catacattaa gcggaaaaga gataagttta ttgtgaaaat cagggggtta catatcatgc
    31141 gcatatcaga acaacaacgg cagatcctcg actatctggc aaaaaacgaa tcaatcaccg
    31201 cacgacaagc cgcagatctc gtttatggtg gcaatgttac ccgcgtccag gttgacgccg
    31261 cccgccgctc actcatgacg atggttagta atgggctaat caggaaacaa ggccgcgctt
    31321 tcgtagctga aagacatatc aatcacgctt atagccagga cgttgaagaa ttgcgccgcc
    31381 agatcctttg ggtattcagc aaacagggtc aggagtattt cctttctgaa cacccaaatt
    31441 tcgccgccaa agagttaaca gtaagcggat tgtcgtgcgc actggattat cttgtcggtg
    31501 aaggttgcgt tacttacaag cagctatggc acgccctgaa tacgcttgta gaagatggtt
    31561 tactcattgc cacgcgtgag aacatcgaat actggataat gccgcgttca ggtcgccgca
    31621 atcatgcacg gtatatttca ggcaacagat acagaacgcc ggactttaat ccgacacagg
    31681 aagatcggga ccgtgccgaa atgatgtgca gggaacagga gaaaagaatt atggataagt
    31741 tattttcgat gcagtgaaca caagcgggcg caatgcccgt ttttattgcc ttattccacg
    31801 cctgacgaat caatttgatc acacctgaga aaatgcatag ccagaagtta aggccgttat
    31861 attgcgattg ggagcgtttt aaacggtatc aatttaatta attctgatgt ggtgatttgc
    31921 aggcaaaaaa aacccgccgg agaagcggga aatagagaca tatcgttgta gttattaaca
    31981 gatcactttg aggtcgaggg cggctaaccc tcgctaatat tatcccgcta ttaccttgcg
    32041 ggtgcaaata atcttttatt gctttgatga tttaataaat agcacttttt gctaacaaaa
    32101 accgctgtca atatatggcc aaaaatgtca acagaaaaaa taagatttat aatattaagt
    32161 atcacatggg taatgggttc agggaatcgg aatccaccgc ctcacggaaa ttcaaaattt
    32221 gcttcttctt tcctccgtgg gcggcttctt tttcgtatcg aggtatttat gaaagcaata
    32281 acattagaac agttcgcagc aatggcgaat gtatcgacca gaacgctgag gcgctggcta
    32341 aaaaaaccat caaccggagt ccctttgcca tttaccccac gcggtactcg ccaattattc
    32401 tgggagcacg aatgcaaaga atggttgtac agaacagaac gataataatc acgcatcaca
    32461 ttttgaggta aacatcatga ccaatatcga aagattagaa cgctgtgccg atattatgcg
    32521 acgcagatgg atttatgacc ctgaagcggg tttattgttt agccgtgaaa caaaagacgt
    32581 tatcaaaggc agcatgacca ataaagggca tttggttgta acggtacatg aaggtaaatt
    32641 caaagtgaca ctcacctatc agaaagcctg ctatgtatac acatacggcg cttatgacga
    32701 gacattatat gaagtgcacc acgttaattt gaacaaacag gataaccgca tcaagaatat
    32761 tcgcctgatg ccgaaagaaa agcaccgccg gatacataaa aacgtccgca aacttattcg
    32821 gctgggccac acacaatggc ttcaaccgca attaattgaa caatcactat aaacacagga
    32881 gtaaatacca tgcattcatt agacagttat tttcagcgca cgacagcccc taaatcagcc
    32941 gcccaggaac gccgtgagga attccatgag aaagtgatgc gttctgctga ttatattgcg
    33001 gacaagttcg tggaaacggt tcgtccactt gttgatgaag tagctgataa acttcagtct
    33061 gaaatgccgg aagatatgga aggcactgca aagcggcgac tcatttgcga attaagtcgt
    33121 cgttttggcg tgtcaataag cgcttttaaa taatcgaaaa aaggggggaa agataatgga
    33181 cgcatttgag gtactggcaa ataaaggcgc tgaactggta gcacagcgtg ataaaaccgc
    33241 caacgaaggg gaacgcgcac tgctaaataa acagattaaa gcgatccgta tggcccagtt
    33301 taaattaatc agtaatgaag ttatcgaaac aattaaaccg cagatcccac aaatcgttgc
    33361 tgacgctatt aaagccgccg gactggagaa acgcattgcc gggctaaaaa cgaacgcaga
    33421 gaaaatggag atgtacaaac agagcggtgc tccgaatccc gatgagtact tctcaccgga
    33481 tgttgagttt atggcgcagg ttgaggaacg tctgggtagc tgtctcaccg aggaacagcg
    33541 tcgctatttt gatggagtgg atagttcggc aggtattgac ctgaactcgt atttcggtcg
    33601 tgaaattgag cattttgatg ccgccccgtc tatgccggag ccagaaccag aaatggccac
    33661 cgatcctgaa gctgagcaac ggaaacgcgt attcaatgcg atagggattc ggtactgatc
    33721 ggataaaaga aagccgggaa ttaccccggc tgacttaaca atgtgaccaa tgaacaaatc
    33781 ggacaagaca aacaggataa gcagtctcga aacaatgttc gggaactata ttagcacaag
    33841 tccggagaaa atcatcatgt tggcactacg catcatgcag ggcatcgcta agaccctcgc
    33901 agaacatgtc ttagacctga aacattcgcc actgagcaaa caggccatga agcgccaaac
    33961 gcttcgtctg tgggctgaat attcgcttgg aactatcaat aaaatcatcg acatgaaatc
    34021 ggggccgagc aaccagagcg cggaggaaat ggagttcatc aggcgtttaa ttctcattcg
    34081 cagagacatt catagccagt tacacagcgt gggaatcgat attaatgatg ggacgggcga
    34141 ttaattttcg gacaagtact atgcgcgcgc gaggagctga acatgaagca agatattgca
    34201 gacaggctgg agatactcga aggccagcgg gcagaagcga aacaactacg taagcaggcc
    34261 agaagagcgc acaggaacaa cgaagcagag ttactaacca aatacatcag tttcaccaat
    34321 tactgcatct acgagtgtta caaggaagat gcagaggact ggctggattc attgccagaa
    34381 cagtactgag cagaataaca ccacgtcatg ttgttctgag gtgagcatat cacctctgtt
    34441 caggtggcca aatttagtgt gccacttcag catcaccgtt gaaatcacaa taacgatttt
    34501 tttgtaaata tgctctcaca tatgtaggaa tagaccaggt tggcattgaa acacatctac
    34561 catgaccata ataaagtaat tttttgatat gtttatggtt ttatattaat taaaatatat
    34621 ctaaatttga tagttttaaa attgcaaaat ccagattgtg tttgtaatgt ttaatctgat
    34681 tgtattgagt ggcgtttatt atgaaaaata aatcaggtaa ttcttgttat agagaagcag
    34741 tcgatattca tagctcactt gagctttcat ttatattgca taaatatcat ttgcgtgtag
    34801 atgagaagaa atctcgaaat gtattcagcg ccatacgtga tgagctcatc cttgatgggg
    34861 attcacgaca aaacttagct actttctgtc aaacatggct tgatgatgaa atccgtgagt
    34921 tgcttaattt agctattgat aaaaacatga tttataaaga tgaatatcca caaatggcgg
    34981 aacctgaagg tcgctgtgtg cgtatgcttt ccgattcgtg gagtttcccc gattcgcgac
    35041 acaccttagg ttgctcaacc attggttcat ctgaagctgc tatgctcggc cgcttggcgt
    35101 taaaatggca gtggtgtaaa aaacgcgaag tacaaggtaa atcaaccgaa cctgatttgc
    35161 gatcctgtgc aaatatgctg gcataaattc tgccgttact gtgatgtgaa attgtgtgaa
    35221 attccacttg atgatcatca cctgataatg aatgctaaag aggttttgaa gcatgtcgat
    35281 gaaaacacta ttggtgtcgt tacgacttta ggtattactt atttttgtaa agcagattgc
    35341 gttagtgttt ttaagataag tgttaaagtt aatattagtt tttatattta catcgtaatt
    35401 atatgtttct attttaaaaa tgttcgagag tataaaaatg tctaatgcta atatcgcacc
    35461 caaaagtaag aaaatgacag tggggacact cgctatcatg aatataactg ctgtagtaag
    35521 tctacgattt ataccttctg aagcagaata cggcttaggt gccatttttt actatacctt
    35581 tgctgcaatt atgtttttag tacctgttgc ctttgttgcg gctgagctag ctacaacata
    35641 tccacaaaaa gggggggcat ttcgatggat aggagaagca tttggggctc gctggggttt
    35701 tgtaggaatg cttatgacct ggctacaagt catcccctac tttccaacag tactgacatt
    35761 tggtgcagtc tcggttgcat ttattgatgt tgatatgggg gtggcagaat caattgccag
    35821 taataaatgg tatattttat ttttcgtact atttgtttac tggggagcta tttgtgttgc
    35881 attacgtggc gttggtattt tctcgcaggt atctaagtgg tgcggtatta tagggacaat
    35941 tattccagct attatccttg ttattcttgg tttctcctat ctgtggttca gcggtaaacc
    36001 agcggtgatc ccattaggtt ggggtgatct tttccctaat atagctaact tccagaatat
    36061 tgttcttgcc gccagcatat ttcttgctta tgcagggatg gaaatgaatg cggtccatgt
    36121 taacgattta gataatccaa ctaaaaaata cccaattgca attaccatcg cttctatagg
    36181 tactattgca atattcctgc tcagtacttt aggggtcgcc tttattattc ctacagataa
    36241 aattagtcta actcaaagtt tgttggttgc ctacgatatg ctgttcgaat gggctggagt
    36301 gccatggctt ggctctgtga tggcatttat gctagcaatt ggcgtactgg gtggggtggt
    36361 tacttggatt gcagggccta atacaggtgt attagcgatc gcgaaagctg gttatttacc
    36421 taagtttttc caaaaaacca atcgtcatgg aatggggcac catctaatgt ttgtacaagg
    36481 tataattgtc tcagttcttt ctgttacttt tgtgataatg ccttcagttc aagcagcttt
    36541 tcagatttta tcacagttaa cagttatgct ctatttagtt atgtatatgc tcatgttcgc
    36601 tagtgctatc tatctacgac attctcaacc tgatacaaaa cgcccatatc gagcccctgc
    36661 tttatttttc tgggcaacac ttggattctt agggtctttt ctggcttttg tattaagttt
    36721 tattccgccc gagcaaatcc caactggtag tccaaccagc tatgttcttt atttgttagt
    36781 attagttgcc ctatttttat taattccaat attaatttac gccttccgaa aatctgactg
    36841 gaaagatcca aactctgatt tcgaaccatt ttcttgggaa aaaagaacta gtaattaaat
    36901 tttccataag ctacaaaaga tgaggttaaa tatgtttagt gctgaatctc caattggtgt
    36961 aatagactca ggtgtcggtg gattgacatc ggtaaaggaa ataattaatt tgcttcctgg
    37021 tgaagatatt atctattgtg gagataatat gaatgcacct tatggcaacc gttctgccga
    37081 tgatatcatc agtttgacta aaaaaatgct gactttttta cagtcaagga atgtcaaact
    37141 agttgctgtc gcatgtaata ctatatcctc aactttggaa tccgaagaat acagcggata
    37201 tgctaagagc tttccgtttc ctatcttatc catcattgaa cctgcagtag aagatgtgat
    37261 tcgccagcaa tataaaaatg ttggtattat cgctaccgag tttactatta aaacggggtg
    37321 tcacaaagag ctaataaaaa aattgaattc aacaataaat gtatttggag aaccaagcaa
    37381 aaatcttgca atgttaattg aggaaggtaa tcttaatgct cctgcaatct tgaatgacat
    37441 caaaaaacat gttaaccatt taatttccct ccatccagta aatgaaatta ttctcggctg
    37501 tactcactat ccaattgttc agaatttttt tgaagcagtt gcaccggata taaaatttat
    37561 taatcctgca catgatcagg ctatctctat taaaaatcac ttgggacagc taaatttatt
    37621 aaactcctca aatataggaa ctttgcaaat aaatactagt ggaagtatgg agatctacaa
    37681 aacggtacta ggggaattga gcattacgaa acctcataca ttcagtatca ggcagtttta
    37741 aatttatccg gtacaccgta aaatcccgtc cttctgaact gcaccccaaa agttggacac
    37801 caactgagta aggtgcattt tatggacaaa cccagttaca tactgggtag tgcatccaat
    37861 tagtagaaca tgtgtttttc gataaacgct ccgatcactt tttcgtggat ctcaactgag
    37921 cgtgagaagc agattgtttt acgacccaac cgcttaatgc gggtgcgtag cgtcagatta
    37981 ttacgctcaa tgcgttgggt gaatattttg ccggtcagat gcttattctt cggcacctcc
    38041 cggccatagc tgccccagtc atcgctggtc agcatgccga tgttgaaggg tgtaagcagt
    38101 gccagtagct cccggcacgt ttgatcggtt cggggaccaa aagtgtaggc cagtacaccg
    38161 cctgttttgg tgttgtacgc gtacccgagc caatgttgcc gggctttact gccaacgtag
    38221 ctccattgct catcaagctc gcagataaac gccacatcag catgggcaac aggcgaagaa
    38281 gttattcgct ttggcgcgag ttttttaaag tccggatgac ggtgttacta ccaattttca
    38341 gtgtcctggc ggtatcgcga accccggcac cattaaaggc catttcagtt atcagctctt
    38401 tcatacccgg cttacgtgct tgataagtat aagtgaactg aaacacacgg tggcagtcac
    38461 ggcagcgaag tctgtcacgg cctttagggt tctgaccatg gcggtaaacc tgagctgact
    38521 gacaacgggg acaatgaata ttaacgctgg tcatgagata acctcaaaag cccgtattat
    38581 acatcagatt caactaatta gaggcatcac ccatccagca ttataaaaca tactaacaat
    38641 aactaaatta taataatcac aacttacaat caaataataa atgcataaga ttaacaatga
    38701 ttttttcggg ttatatttat ctaaaaaatc agaacattat gtaacttatt attttctaag
    38761 cgaaaattta ttgtgcggtc agactaacaa acttgcgtac cacccgaaga acatgcgaag
    38821 atttaagtat tctttctttt ttggttttta tttttcattt tctcttttct tctctttccg
    38881 gttctgttct ggttttggtt ttttcctttt tatttttatt ctttcttcag ggttatgcta
    38941 aaacaccaaa acatttttca ccacaaaagc atccaatcat cttgccagac caatcacctt
    39001 aaccggatac actgccggat ataatcccac taacacccag acaccaagca ccatacctga
    39061 taactaccgt attcaggaag gaaatgcgag tgatttcacc gtcaccggca caaccgatcc
    39121 gccaaaaaga ggcgtaccaa tgtcgatata gtcccccgcg cggacaatca cttcgtcaat
    39181 gactgccaac gggagttgtt gtagctgtgg gcgcaacaac atgcccagag ctttaccaaa
    39241 gtcctgcccg gccaccacca gcaggggatg cggattcgga aaacgcgcga cgaaatcgac
    39301 cagcgcgttg atgaccgtca gaaccgcggc gtaacgcaca ggcagcgagg cgggaagcgc
    39361 cagcacgtac acgtcagttt tgggatcaag atccagctga agcagcgcct gttgccaggc
    39421 actcaccaga tccgtttcat caatcgggat cgccaccggc aaattgcgca gcggcagttg
    39481 tacgccctcc agccagattg tgctgccaga gagcgaaagg gtatgtgcac ccgcgccaat
    39541 caccgtggag cgtacggttt gcgccggaaa ctgcacattc atctcacgca ggcgcggatg
    39601 gtcatgcagc gccgttgcca gaagtgggcc aatatcggca aaacagaacg ggttggcggg
    39661 ctggtggcga taacattcgc ccacgcctcc agaaagcgta atgatttcgg gcgtaacacc
    39721 tgcgggcagc aaaccggttt gcatcaatgc ctgcgcgagc ggcgaaagcg ttccgtcaat
    39781 cacttcgaca atcagcgccg ccatccgccg ggtaacctgc accagctgcg cgccggtcag
    39841 cgaacgggcg tcagtgcctg caccgaagca ctcatccaca atcatctgcc ccggtttatg
    39901 agcgtaaacc acgcgcccct ggctgtcggt ttccagcagg cgaccaccga cgttgagaca
    39961 ggcagtgccg ctgatttttc cggcatcgaa cagggcgtag ttcgcggtgc caccgccgat
    40021 atcgatattc agtacccgac acagccgttg ttcagaaagg gtttgcgccc cggctccgtg
    40081 accggcgatc acggattcga ggtgcggccc ggcgctggcg acgacaaaat cgcccagcga
    40141 ctgtttttcc ttcatcactt tttccatcag ctgtgcgacc aggctttcgg taaactggcc
    40201 ttccggcagc tgggcaatga tggtttcgcg gatgcgctgg ctttcggttt tgtcggctgg
    40261 cacgctggcg ggcacggacg ccgttaccgg gatcgattca tcacattcgg tgatggtaaa
    40321 acccagtaga tccgccactt cacgggcttc cggggtaata atgctggcgc gcagaaccac
    40381 ggacattgcc tgttcgccgc gcgcgtgtgc ttcacgaata tcattcgctg tgatgagttt
    40441 tttcacctgc ttcctcctgt ttggtgagag atgccagata atccaccagt tgctgcacgc
    40501 tttgcgggtc gtggctgttt agctcgaaaa tcggctcttc gaaacccgtc tcaagcaata
    40561 actttcgcgt tgcggcgacg tcagcatctg gcatgtccgt tttactgatg acggcgattt
    40621 gccgtttgct gacgccaata tccagcaacc cggcaggcaa ccgactttcc gggtcatttg
    40681 cgccatggac atagatcagc atgtctacat cctgtagcgt ggtaattaag gcgtgatacc
    40741 agcggggatg gctaaaatat tcacccggcg tatcaatatc gcccttatca ttaaattcca
    40801 cggccagtgt ttttctggcg agggtataat tcccctgcaa cgcattaaat aacgttgttt
    40861 ttcctgcccc gacgctaccc acaaacgcaa ttcgtttcat gaatgcctcg tattaacttt
    40921 tggtcatttc acaaagcgta taatttaata agcgacctaa accgctgacg gtttgtgata
    40981 acgcttcttc tactgcgccc accgagccat aaatcaccaa tgcaccgctg aagcggtcga
    41041 gaaaaccgat atgcacatca gcggctttca gcgctaaatc accggcgatc atcgccgttt
    41101 cgccgggagt tagcgtcata atgccaattg cgcccgcatc cggaacgccg attttcttcg
    41161 ccagttcctc accgggatgc gcgatgagat gcgccagtgt gacctgtttg ccgggcacaa
    41221 attcctgaat gatgcgttct ttatccatga gtcgccgcct gtgaaaaact ttcgtgtatc
    41281 gtgacaattt ttcgtcagag taaataacaa aattcggcaa gcgcgtttaa aaggtgaggt
    41341 agatcaataa gaatggagag atatttactg ttatgaataa actttcatat gcgacgcatg
    41401 aatataaata aaataatatc gagatatttt atatactgat ttttttatta ttcgcaaact
    41461 cagtaataag ctttgataat atatttcagg aaaaccgctt accctgaata ttcagggtaa
    41521 gcgtgagagt taagaatgaa ttacagcggt tgggtttgcg cttcaaccac ggccagcgcc
    41581 accatattga cgatacgacg caccgatgcg atcggcgtta acacgtgaac cggtttcgcc
    41641 acgcccatca gcaccggacc gacagtcaca ccttccgagc tggaaacgcg cagtaagttg
    41701 taactaatgc gggcagcttc catgttcggc atcaccagaa tattggcaga acctttcaaa
    41761 gggctgtccg gcatacggtc gttgcgaatc gcttccacca gcgctgcatc gccgtgcatt
    41821 tcaccatcaa tcatcagatc tggcgcacga tccctgacca gttccagcgc ctgacgcatt
    41881 ttgctcgacg acgggcagtc agaagaacca aagttggagt gcgacaacag agcaacgcgc
    41941 ggctcaatac caaaacgacg gacagtttct gccgccatca aggtgatctc cgccagctct
    42001 tctgcgtctg gttcgtcatt gacgtaggta tcggcaataa aggtgttacc actcggcagc
    42061 agcagcgcat tcattgcacc tgcggtgtga acgccatcgc gataaccaaa gacatttttc
    42121 accacactaa aatgttcatg ataatcgccc accgtaccgc agatcattgc atcggcttcc
    42181 ccacgctgaa ccatgatcgc gccgatcact gtcgggttac tgatcagcgc acgctgcgcc
    42241 tgttcctgag tgacaccgcg acgcttcatg atctggaagt attcggtcca gtactcttta
    42301 aagcgcggat cggattcgtt attgacgatt tcaaaatcaa cgcccgcttt gatctgcaag
    42361 cccagtttct gaatgcgcat ttcgatcacg ttcggacgac cgataaggat cggtttcgcc
    42421 agtcccagcg ttaccagttc ctgagtcgca tgcagaacgc gcgcctcttc cccttccggc
    42481 agaactacgc gcttcggcgc tttgcgagcc tgggagaaaa tcggcttcat aaacaggttg
    42541 gttttgtaaa cgaactcagt cagcttgtcg atgtagacgt cgaaatcagc aatcggacgc
    42601 gtcgccacgc ccgactccat cgccgctttt gcgaccgcag gagcgatctt aacgatcaag
    42661 cgcggatcaa acggttttgg aatgatgtat tccggaccaa agctcagatc ctgaacgcca
    42721 tacgctgaag ccaccacttc actctgttct gcatgggcca gttctgcaat cgcgcgtacc
    42781 gccgccagtt tcatctcttc gttgatggcg gttgcgccaa cgtccagcgc gccacggaag
    42841 atgaacggga agcaaagaac gttgttcacc tggttcgggt agtcggaacg gccggtacag
    42901 atgatggcat ccggacgcac ttctttcgcc agcggcggca gaatttccgg ttccgggttc
    42961 gccagcgcca ggatcattgg tgcccgagcc attttcttca ccatttcctg ggtcagcact
    43021 ttcgggccgg aacagcccag gaaaatatcc gcaccttcaa tcacatcatc gagggtacgt
    43081 ttgccgtcat ccaccaccgc atatgcggct ttggtttccg ccatatttgg ctcacggccc
    43141 tgatagataa cgccttttga atcgcaaacc acgatgttat gtttttgcag ccccagcgct
    43201 accagcaggt tcatacaggc gattgctgcg gcacccgcgc cggaaaccac catccgcacg
    43261 tcggagatgt ttttctccac cacgcgcaag ccgttgagga tggcggcagt gctgatgatt
    43321 gccgtgccgt gctgatcgtc gtggaatacc ggaatattca tccgctcgcg cagtttctgt
    43381 tcaatataga aacattctgg cgctttaatg tcttcgaggt tgatgccgcc gaaggtcggt
    43441 tcgagcgcgg cgacgacttc aataaatttg tccgggtcga gttcatcaac ttcaatgtca
    43501 aatacatcaa tcccagcgaa tttcttaaat agaacgccct tgccttccat caccggtttg
    43561 cctgccagcg cgccaatgtt gcctaacccc agcaccgccg taccgttaga gatcaccgca
    43621 accaggttac cgcgcgcggt atatttgtag gcttttagcg ggtctttttc gatttcaaga
    43681 caaggtgcgg caacgcctgg tgagtaggcc agcgccagat cgcgctgcgt tgccagaggc
    43741 ttggttggag aaacctggat tttccctgga actggaaatt catggaaatc aagtgcactt
    43801 tgttttaact ggtcatccat ttggttgttc ctttcacgta acgttcacaa ataaagtgtg
    43861 tgggcaacag cccctgccca caacgtggcg cacattatta ccctgccgga gtctacagac
    43921 tttgagcaag tccaaactct caccattaat ataatgtttt ggtaataatc ctataacact
    43981 gatgttacct gcttaatcca gcaataccat gcctgtctgc tatgcttttt tgatgcgttt
    44041 agcgaaattt ctcagaagtg tgaattaacg cactcatcta acactttact tttcaaggag
    44101 tatttcctat gaacgagtta gacggcatca aacagttcac cactgtcgtg gcagacagct
    44161 gcgatattga gtccattcgc cattatcatc cccaggatgc caccactaat ccttcgctgt
    44221 tactcaaggc tgccggatta tcacaatatg agcatttaat agacgatgct atcgcctggg
    44281 gtaaaaaaaa tggcaagacc caggaacaac aggtggtcgc agcgtgtgac aaactggcgg
    44341 tcaatttcgg tgctgaaatt ctcaaaatcg tacccggttg cgtgtcaaca gaagttgatg
    44401 cacgcctctc ttttgataaa gaaaagagta ttgagaaggc gcgccatctg gtggacttgt
    44461 atcagcaaca aggcgttgag aaatcacgca ttttgatcaa actggcttcg acctgggaag
    44521 gaattcgcgc ggccgaagag ctggaaaaag aaggtattaa ctgcaacctg acgctgctgt
    44581 tttcttttac ccaggcgcgg gcctgtgcgg aagcaggcgt ttttctgatt tcgccgtttg
    44641 tagggcgtat ttatgactgg tatcaggcac gcaagccgat ggacccgtat gtggtggaag
    44701 aagatccggg cgttaaatcg gtgcgcaata tctacgacta ctataagcaa caccactatg
    44761 aaaccattgt gatgggcgcg agcttccgtc gcaccgaaca aatcctcgcc ttaaccggct
    44821 gcgatcgact gactatcgca ccgaatttgc tgaaggagct gcaggaaaaa gtttctccag
    44881 tggtacgtaa attaatcccg ccttctcaga cgttcccacg cccagcaccc atgagcgaag
    44941 cggagttccg ttgggagcac aatcaggatg cgatggcggt agaaaaactg tctgaaggca
    45001 ttcgtctgtt cgctgttgat caacgcaaac tggaagatct tcttgccgcc aaactataaa
    45061 ccagccacgg agtgttatat gtcccgaaaa gaccttgcca atgcgattcg cgcactcagt
    45121 atggatgctg tacaaaaagc caattctggt catcccggcg cgccgatggg catggctgat
    45181 attgccgaag tgctgtggaa cgattttctt aaacataatc ctaccgaccc aacctggtat
    45241 gatcgcgacc gctttattct ttccaacggt cacgcgtcga tgctgctcta cagtttgcta
    45301 catctgaccg gttacgacct gccgctggaa gaactgaaga acttccgtca gttgcattcg
    45361 aaaaccccag gccacccgga gattggctat acgccaggcg ttgaaaccac caccggcccg
    45421 cttggacaag gtttggcgaa cgccgtcggg ctggcgatag cagagcgtac actggcggcg
    45481 cagtttaacc agccagacca tgagatcgtc gatcacttca cctatgtgtt tatgggcgac
    45541 ggctgcctga tggaaggtat ttcccacgaa gtctgttcgc tggcaggcac gctgggacta
    45601 ggcaagctga ttggttttta cgatcacaac ggtatttcca tcgacggtga aacagaaggc
    45661 tggtttaccg acgatacggc aaaacgtttt gaagcctatc actggcatgt gatccatgaa
    45721 atcgacggtc acgatccgca ggcggtgaag gaagcgatcc ttgaagcgca aagcgtgaaa
    45781 gataagccgt cgctgattat ctgccgtacg gtgattggct ttggttcgcc gaataaagca
    45841 ggtaaggaag aggcgcacgg cgcaccactg ggggaagaag aagtggcgct ggcacggcaa
    45901 aaactgggct ggcaccatcc gccatttgag atccctaaag agatttatca cgcctgggat
    45961 gcccgtgaaa aaggcgaaaa agcgcagcag agctggaatg agaagtttgc cgcctataaa
    46021 aaggctcatc cgcaactggc agaagagttt ccccgacgga tgagcggtgg tttaccgaag
    46081 gactgggaga aaacgactca gaaatatatc aatgagttac aggcaaatcc ggcgaaaatc
    46141 gctacccgta aggcttcgca aaatacgctt aacgcttacg ggccgatgct gcctgagttg
    46201 ctcggcggtt cggcggatct ggctcccagc aacctgacca tctggaaagg ttctgtttcg
    46261 ctgaaggaag atccggcggg caactacatt cactacgggg tgcgtgaatt tggcatgacc
    46321 gctatcgcca acggcatcgc gcaccacggc ggctttgtgc cgtataccgc gacgttcctg
    46381 atgtttgttg aatacgcccg taacgccgcg cggatggcgg cactgatgaa agcgcggcag
    46441 attatggttt atacccacga ctcaattggc ctgggcgaag atggtccgac gcaccaggct
    46501 gttgagcaac tggccagcct gcgcttaacg ccaaatttca gcacctggcg accgtgcgat
    46561 cagatggaag cggcggtggg ctggaagctg gcggttgagc gccacaacgg accgacggca
    46621 ctgatcctct caaggcagaa tctggcccag gtggaacgta cgccggatca ggttaaagag
    46681 attgctcgtg gcggttatgt gctgaaagac agcggcggta agccagatat tattctgatt
    46741 gccaccggtt cagagatgga aattaccctg caagcggcag agaaattagc aggagaaggt
    46801 cgcaatgtac gcgtagtttc cctgccctcg accgatattt tcgacgccca ggatgaggaa
    46861 tatcgggagt cggtgttgcc ttctaacgtt gcggctcgcg tggcggtgga agcaggtatt
    46921 gccgattact ggtacaagta tgttggtctg aaaggggcaa ttgtcgggat gacgggttac
    46981 ggggaatctg ctccggcgga taagctgttc ccgttctttg gctttaccgc cgagaatatt
    47041 gtggcaaaag cgcataaggt gctgggagtg aaaggtgcct gatggtgatc gccggatgct
    47101 gtatgttccc ctcaccctaa ccctctcccc agaagggcga ggggacggtc cgtgcgcttt
    47161 ttagggccgc atctgcgatt ttgtttaacg ggtgatccac aacgtgggcc tggcgtctgg
    47221 cccatgccag ttatcgcagg taggttctgc ggcgtaacgc accaggcgaa aacgctgacc
    47281 gtcaaagcgc cagcgcgcct gaatgccaca atcgcttaat ccgcgccctt tcgctaaggt
    47341 caccagttca cgcgatttct catcaaatgt tgcgttcatc agttccagtt cattcgtctc
    47401 ctggccgttg ttgaacggca aacgcaaccg aaccgggcgc gaggctagcg gctttttacg
    47461 cgacacaatc catgccaaat caatggtgtt ataggcccct gcctcacagc taatcatcat
    47521 cagtgcttta tcatcggtca gcgcagtgac attcacctca cgacgtaacg gatcaagcga
    47581 gcagcgcaga ccattcatcc gccagtttcc ataatccagc aaatcgttac gttcttcgag
    47641 tgagagtggc gtcggcgttg ggttaaccac cgcgacctct ttcagcgcag gcgcgggcgg
    47701 tacgctgaga ggcggttcgt cccctttctt gatccacgcg gtttcactgc caacgcgctt
    47761 ttgctgagca tcaataaaca acaacgccgc ttttaagccg ctcagagaaa tggtctgatt
    47821 gccgtcgcgt aaggtgatcg ccttcccttc ctgaattatc tgcaaaaacg cggtgatggt
    47881 tgccgtatca tcggttacta ataaccatgg tgaaatccgc cacttgtcac cacttaatgc
    47941 caacggctca ccatctaata gcagccgtgg cgctatctcc ccttcagacg catccggcga
    48001 cttcaaccca ccgcgctcaa tacgtaaaac ggcatcggta tgcgccccag cgctgcggct
    48061 cagggtcatc accagtccat tatgatcgcc cgtattgcgc gccacacaaa aattttgatt
    48121 attacaggtg acctgccagt cggaaaacgc ccgttgcgct ggtgccgccc acaccaaaga
    48181 cgtcggcaac aaagcgaaaa aaaagagaag gaaaatgcga tagcgcatgg acggtacgac
    48241 cccagaagtt caaacaaaca gcacaagtcg tatcttcgtt gcgcaggtag cgcggctcaa
    48301 tcggatttgt cggattgagc caataataaa caacgaaata ttattgctat ttttcagtcc
    48361 attaaatgtg acatttgcaa atagttaagc aataacaccg tcttaccgtc acgtatctcg
    48421 ccggttttga tcatctccag cgcctggctg aacggcagct cgagcacttc aatatcttca
    48481 tcttcaacac cgccaccggc gttagcgcgc tgattgtcac tgtattcggc gataaaaaag
    48541 tggattagct cagtcacacc gccaggcgac atatacagtt caaataattt gcgcacttcg
    48601 cccacttcat agcccgtctc ttcaatcgct tctttacgaa tgcacacttc cggttcgtcg
    48661 ttatccagca gcccggcgca ggtttcaatc agctgcccgc tttcattgcc attaacccag
    48721 gtagcgacac ggaactgacg aatcagaacc acgctctttt tcttcgcgtt gtacaggagg
    48781 atcgtcgcac cattaccgcg atcgtaaact tcacgtttat ggcggataac ttcgccatct
    48841 ttgcgcgtga gatcgtaagt aatgttgtgc agggtgaaat agttatcgga gagaattttg
    48901 tctttaatga gggtgatttg ttgcgtcata ccgactccac agcgcgaaat gaacaattat
    48961 attacgctgt gaagtcgggt ttgtctgcgc aggctatcag tgagatttga ctgattttac
    49021 ccccagccag tcaataatcc cctgtgccgc atgacgtcct tctgccattg cggtaaccac
    49081 cagatccgca ccacgcacgg cgtcgccacc ggcgaagatt ttcggattgg tggtctggta
    49141 acggtactgg ctttccacat ccgcgatgat gcggccccat ttgtctaccg ttacaccgtg
    49201 cgactccagc cacggcatcc cgtgtggatt gaagccaaac gccataatca ccgcatcggc
    49261 tggcatgaca aattcactgc cctcaatcgg tacaggtcgc cgacgcccct gggcatccgg
    49321 ctcgccaaga cgcgtgcgca ggaaacgaat tccgcaaacg tgaccttgct cattcagctc
    49381 aagcgccacc ggctggacgt taaattcgaa gttggccccc tcttcgcggg cgttcttcac
    49441 ctctttcttc gagcctggca tgttagcttc atcacgacga taagcgcagg tgacgttact
    49501 cgcgccgtgg cgcagcgcag tacgcacaca gtccatcgcg gtgtcgccgc cgcccagtac
    49561 cacgacgtta agtccggcgg tattgataaa cggctcttcc ggtagctctt cgagccccat
    49621 cacctgttta gtgttggcaa tgaggaacgg cagcgcgtca taaacgcccg gcgcatcttc
    49681 attgggtaaa cccgctttca tggaacggta agtgcctacg ccaacgaaga ccgcgtcgta
    49741 ttgttccaaa agcaaatcca aagagacatc tttacccact tcacaattga gttcgaagtg
    49801 aatccccatc gcgctgaaga tttcccgacg gcgtgccagc agggatttat ccagtttgaa
    49861 agaaggaatg ccgaaagtga gcaagccacc gatttctgga tggcgatcgt acaccgtcac
    49921 cgccacgcca ttgcgggtca gaacatccgc acaggccagc cctgccggac ctgccccgat
    49981 aatcgccacc cgcttgtcca ctttggtgac atgacttaag tcaggacgcc aacctttcgc
    50041 caacgcctga tctgaaatat agcgttcaat gttgccgata gttaccgcgc cgtgctcatc
    50101 gcgaatagta caggcacctt cacacaaacg gtcttgcgga caaacgcgtc cggtaatttc
    50161 cggcagggtg ttggtctggt gagaaagctc gacggcggcg tcgatgtttc cggctttcac
    50221 cagttcaatc cactgcggaa tatggttatg cagcgggcag gtccattcac aaacgctgtg
    50281 ctcgccgaac ttaaggcagc gcgaggcttc ccgttgtgcc tggtcggcgc gaaatggcag
    50341 ataaatttca tcaaaaccgg ttttgcgcgc ttcaatcgcc agtttatccg gctcgccacg
    50401 cgcgggcgtt gcctgcattt gttcgacttt actcattacc ggcatttctt gtgcagcggt
    50461 actggcatgc cacggttgat gctcctgacg tgcggtgcgc aagcggcggg atttcgccat
    50521 gccggagagt gcgacgtcag tgaccagttg cagcgcgtcc gccgggcaat tctcgacaca
    50581 ggcaggaccg ttttcgcgcc ctgcacaaag gtcgcattta tgcgccgtgg cttttacttt
    50641 tcctgccgcg acgggtgtca ggacgatttg catcgtacca aacggacagg ccaccacgca
    50701 ggatttacag ccaatacact tttgctgatt gacctgaatg ctgtcatcaa cgtggctgat
    50761 tgcgccatta gggcagctac gggcgcaggg cgcatcttca caatggtgac aggtcactgc
    50821 actacgttgc tgctgatgtt tgataaccgt aattcgggga tgaaaatggt gttggctcag
    50881 gacatgttgc tcatcattgt gagccatgac acaggcgatt tcacaagcat gacaacccag
    50941 acattgctga ctgttggcca taataaaacg attcataacg accttctttt ttggttgtaa
    51001 aaaccttatt ctttatatga gtgttgttat tacccgactt acaggggaac cggcaatgtt
    51061 catgtgccca gaataagtaa ctatttcgct ataaactgtg gcagatcaaa taatcccatc
    51121 actgactaaa ttgcgtttca gcgaactgga acattaatga ttttttgtgg agaagacgcg
    51181 tgtgattgtt aaacgacccg tctcggccag tctggcccgg gccttttttt acattgtgct
    51241 gctgtcgatt ctttccacgg gtatcgctct gctaactctg gcgagcagtt tgcgcgacgc
    51301 tgaggctatc aatattgccg gatcgctgcg tatgcagagt taccgcctgg gctacgactt
    51361 gcaaagtggc agtccacaac tcaatgcaca tcgccagttg tttcagcagg cactgcattc
    51421 accggtatta accaacctca acgtctggta tgtgccagaa gcagtaaaaa ctcgctatgc
    51481 gcatctgaat gccaactggc tggagatgaa taatcggctc agcaagggcg atttgccgtg
    51541 gtatcaggcc aatattaata attatgttaa tcagatagac ctgttcgtgc tggctttaca
    51601 gcactacgct gaacgcaaaa tgctgctggt ggtggcgatt tccctggctg gcggtatcgg
    51661 tattttcacg ctggtctttt ttactttgcg ccgcatacgc catcaggtgg ttgccccgct
    51721 gaatcagctg gttaccgcca gtcagcgtat tgaacacggg cagttcgact cgccgccgct
    51781 ggataccagt ctgccgaatg agcttggtct gcttgcaaaa acctttaacc aaatgtcgag
    51841 cgagctgcat aaattgtacc tttcgctgga ggcgtcagta gaagaaaaga cccgcgatct
    51901 ccacgagacc aagcgtcgtc tggaggtgtt gtatcagtgc agtgttccca ggcgctgaac
    51961 accagccaga ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa
    52021 gcagctgaat atctggagct gaacgttggg gataactggc ggattagcga agggaagcca
    52081 aaccctgagc tgccaatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa
    52141 ctgcactggc aaaatagtca cgtttcatca tcagaaccgc tgcttaacag tgtttcatcg
    52201 atgctgggac gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg
    52261 atggaagaac gtgcgaccat cgcccgcgaa ttgcacgact cgctggctca ggtgctttct
    52321 tacttacgta tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca
    52381 caaagtatca tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag
    52441 ctgttgacta ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa
    52501 atgctggata cgttacaaaa tcaaaccagc gctaaactga ccctcgactg ccgtctgcca
    52561 accctggcac tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg
    52621 ctgaatgcga tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg
    52681 gacggcaatc acacggttta tatccgtgat aacgggattg gtatcggtga accgaaagaa
    52741 cccgaaggtc attatggact gaatatcatg cgcgaacgcg cggaacggct gggcgggacg
    52801 ctgacttttt cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctccgca
    52861 gagggtgagg ggaaggggaa cacgccggct gaggtgtaaa taattaccct cacaccggcc
    52921 ctctcccttt aaaggcgaga gggaaaaacg tgcccatatt gacctgtaag cgagtgtgtc
    52981 agcaatctca tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg
    53041 ccgctacagt gaagcaagtc aagcctacaa caatacgcag aaacacgagg tcctctttta
    53101 atggcgaatt tctttattga tcgccccatt tttgcctggg tgctgacaat cctgttgtgt
    53161 ctgacaggta ccctggcgat tttttcattg cccgttgaac aataccccga tctcgcgcca
    53221 ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc
    53281 gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctgatgta tatgtcatct
    53341 cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg
    53401 gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag
    53461 gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaacat tctgaccatt
    53521 gccttcgtct ctaccgatgg ctcgatggat aaacaggata ttgctgatta tgttgccagt
    53581 aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg
    53641 caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct
    53701 aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt
    53761 ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg
    53821 caaacaccag aacagttccg cgatatcacc ttgcgggtga atcaggacgg ctcagaggta
    53881 aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc
    53941 ttcaatggta agccagcctc cgggttgggg gtaaaactgg cctccagcgc aaacgaaatg
    54001 gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcacgga
    54061 ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg
    54121 gtgaaaacgc tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa
    54181 aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc
    54241 tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg
    54301 gcgatcggtc tgctggtgga tgacgccatc gtggtggtgg aaaacgtcga acgtattatg
    54361 agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg
    54421 gcactggtcg ggattgcgat ggtgctttcg gcggtatttg taccaatggc cttcttcggc
    54481 ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg
    54541 tcagtactgg tagcgatgct cctcactccg gctctgtgtg ccacgctact taagccactg
    54601 aaaaaaggtg agcatcacgg gcaaaaaggt ttttttgcct ggtttaacca gatgtttaac
    54661 cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg
    54721 attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg
    54781 tcgttcttac cgctggaaga ccggggcatg tttactacct cggtacaatt gcccagcggt
    54841 tcaacccaac aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat
    54901 gaaaaagaca acatcatgtc ggtgtttgcc accgttggtt ctggccctgg gggtaacggg
    54961 caaaacgtgg cgagaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc
    55021 ggcacctcgt ttgccattat cgagcgtgca acgaaggcgt ttaaccaaat taaagaagct
    55081 cgcgttatcg ccagcagccc accagcaatt agcggtcttg gtagttctgc aggttttgat
    55141 atggagttgc aggaccacgc tggagcgggt cacgatgcgc tgatggcagc acgtaatcag
    55201 ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgat
    55261 gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgat
    55321 atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt
    55381 atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg
    55441 ccagatgaca tcaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct
    55501 gctttcgcga cctcacgctg ggaaacaggc tcgccgcgtc tggaacgcta taacggttat
    55561 tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat
    55621 attatggaat cgttagtgaa gcagctgcca aacggctttg gtctggagtg gacggcgatg
    55681 tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg
    55741 gtggtattcc tgtgtctggc ggcattgtat gaaagctggt cggtaccgtt ctcggtaatg
    55801 ctggtcgtgc cgctgggggt aatcggcgcg ctgctggcaa cctggatgcg cgggctggaa
    55861 aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc
    55921 atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccatgacct gtttgaagcg
    55981 acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc
    56041 ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg
    56101 ggtactggcg taatgggcgg gatgatttca gctactattc tggctattta cttcgtgccg
    56161 ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata
    56221 aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc
    56281 gggttcttgc tgtttatact ttgtctcagg aattacttac gaagcataac ttcgataaag
    56341 tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt
    56401 ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc
    56461 gggggacaaa ccttatgtaa atactatggt cctgcggtga taatttgtat gtgatacaca
    56521 gcaacatttc gagatattca tacggcatct aatactgatt taattctggt taaaatacag
    56581 acagataaca agatgaatat tcttaatgtt tacgttaaaa atgtttaata ttatttaata
    56641 gttgttaatt tgaatacttc gataatgtta tattttctga taatcatttg caggcaaaat
    56701 gttttcaccc ttaaatgagt atttattctc ataaatcgaa aaaggattca ttatggttac
    56761 actttacggc atcaaaaatt gtgacaccat taaaaaggct cgccgttggc tggaagccaa
    56821 taacatcgac tatcgttttc atgattaccg cgtcgatggg ctggacagcg aattattgaa
    56881 cggttttatc aacgaattag gctgggaagc gttactcaac acccgtggta caacctggcg
    56941 taaactggac gaaactaccc gcaataaaat caccgatgcg gcctctgcgg cggcattaat
    57001 gactgaaatg cctgcaatta tcaaacgtcc attgctctgc gcgcctggta agcctatgct
    57061 gctgggtttc agtgattcca gttatcagca atttttccat gaggtgtagt ctatgtcgtg
    57121 cccggttatt gagctgacac aacagcttat tcgccgccct tccctgagtc ctgatgacgc
    57181 aggatgtcag gctttgttga ttgaacgttt gcaggcgatc ggttttaccg ttgaacgcat
    57241 ggattttgcc gatacgcaga atttttgggc atggcgtggg caaggtgaaa cgttagcctt
    57301 tgccgggcat accgacgtgg tgccgcctgg cgacgccgat cgttggatca atccgccgtt
    57361 tgaacccacc attcgtgacg gcatgttatt cgggcgcggt gcggcagata tgaaaggctc
    57421 gctggcggcg atggtggtag cagcagaacg ttttgtcgca caacatccca accatacggg
    57481 gcgactggca tttctgatca cttctgatga agaagccagt gcccacaacg gtacggtaaa
    57541 agtcgtcgag gcgttaatgg cacgtaatga gcgtctcgat tactgcctgg tcggcgaacc
    57601 gtcgagtatc gaagtggtag gtgatgtggt gaaaaatggt cgtcgcggat cgttaacctg
    57661 caacctcacc attcatggcg ttcaggggca tgttgcctac ccacatctgg ctgacaatcc
    57721 ggtacatcgc gcagcacctt tccttaatga attagtggct attgagtggg atcagggcaa
    57781 tgaattcttc ccggcgacca gtatgcagat tgccaatatt caggcgggaa cgggcagtaa
    57841 caatgttatt ccgggtgaac tgtttgtgca gtttaacttc cgcttcagca ccgaactgac
    57901 tgatgagatg atcaaagcgc aggtacttgc cctgcttgaa aaacatcaac tgcgctatac
    57961 ggtggattgg tggctttccg ggcagccatt tttgaccgcg cgcggtaaac tggtggatgc
    58021 ggtcgttaac gcggttgagc actataatga aattaaaccg cagctactga ccacaggcgg
    58081 aacgtccgac gggcgcttta ttgctcgcat gggggcgcag gtggtggaac tcgggccggt
    58141 caatgccact attcataaaa ttaatgaatg tgtgaacgct gccgacctgc agctacttgc
    58201 ccgtatgtat caacgtatca tggaacagct cgtcgcgtga tgagtggttc tgcaagagga
    58261 aataagcatg gactggctgg ctaaatattg gtggattctg gtgattgtct ttttggtagg
    58321 cgtcctgctg aacgtgatta aagatctcaa gcgcgtcgac cataagaaat ttctcgccaa
    58381 caagccggag cttcccccgc atcgtgattt caacgataag tgggacgatg acgacgactg
    58441 gccgaaaaag gatcaaccga agaagtaagc tgagtgctca accgtaggcc ggataaggcg
    58501 ttaacgccgc atccggcaat ggtgaacgat ccctgatgcg acgctcacgc gtcttatcag
    58561 gccaggtaat ctgtctttta catcatttca atcacatcat catcgccagg cttaccgcca
    58621 cttagcgctt ggtcgaaata atgtttcgga atggtataac gcaagtgatc cagggcaaat
    58681 tgcatactgc gattgtcgat cgcatggcct aaatcctcca cgatatccag cgtcacgtca
    58741 ccaccggcac tgattaacgc ttcctgcgcc gcaactgcat gcgccagatc gataaccgga
    58801 tcttcaccac catgaatcaa gtgaattgtc gtggctgtag acgcggtctc cggcaggctc
    58861 gcatagcgtc cgttaaaggc aatgacgcgc gaagcaaggc ccggttcggc tttaatgctc
    58921 tccagcgcca taatcgcgcc ttgtgagaaa ccaattagcg ccgtggcgtt agcccctact
    58981 ccgctctgtt tctgccagta gcgtaccgtt tcaataaacg tcggcatgat ggcatccaca
    59041 cgcgcctggc gattatcttc cgtaataccc tgcactgaaa accactgacg ccccgccgga
    59101 ttaccgctcg gctccgcgcc accgacactc accaccagcg catcaggaaa cagcggtgca
    59161 aaccagctgc ctatttcccc cattgccacc gggttatccc cgacaccgtg gaaaagcagt
    59221 aacaactgtt gtgcaggttt atccgggctt tgaacaacaa aatggtcatg tttcatggcg
    59281 aactccttaa ctgatgtcat taattttacg ccgctgactc acaatgacca tgggagaaaa
    59341 ttgaaggagt cagtgaaaaa attgccattg cgttatgcga tcgcgcagac gctcagtgcg
    59401 aacctcattt agtgcgaaca gcgcctgcgc ggcctcttcc cgctggcgaa ccagtaacat
    59461 cttgcggcct gaaagtttaa gtgtggtaca taactgcgcg tcactggcgt ttttctgtaa
    59521 acgcccacgc agcgccggta atgccagttc actggtttgt agcagacgca ataagcaacc
    59581 taacgatgtt aatagcggac gatgagcgaa agcaaaaccg gccagttcaa gccagtcgtc
    59641 gtcagaaagg atggcatcgt ttagtggatc aacaggaagc atttcgccat tccactgcgc
    59701 gagagcttgc gcatcgcgac gtaaacggta atgctcacgt tcagccagct gtttacccgc
    59761 atcactcatc ggtaacagcg ccatcgccgt atagcaaccg ctgctggctt cccgatgatt
    59821 acccatccgc accagcacaa aaccgcagcg ttgccagaaa cgccataact ccccagtgta
    59881 accaaaactc accgaaagat agtcgaggtc atgcgtatat tgcaaagctc cagcaataag
    59941 ttgtcgccct gtgccttccc gctgacgagc cggatgaact gctatccggc taacccgcct
    60001 tccgcgcaat gtcgccgcca gtggattgct gccgtgcgcc gccagcgact gggccaccag
    60061 attaccccgc gggcgacgaa aacctgccca taccgcctga ctgagttctt gagataatcc
    60121 gccctcatcc accagccaca acgccccggc aatctcgttt tcgccagccg cctgtaaaaa
    60181 atgttgccct ggtgcatcca tcatccggcg taaatccagc ggcgaggtcc ggtagtgcgc
    60241 accagacaat aactgataaa cctttaacgg cgtttctggc tcgcttcgcc ataacgtttg
    60301 ttcaaatgcg gaaatgacga tattgccttg tggtgtatgg gtgaagtttt catcgttaaa
    60361 aaccagtgcc tcactaacca ttttttccag cgggcatccc tgtgcccagc ggatcggctg
    60421 ttgcagttca aaacggtgta aatgcggaaa gcgagcgcaa aatttcagca aaaaaccacg
    60481 tccggtacct tcgtagccct gcaccgtagt ggttaacaac gttcgaggaa aacgcgatac
    60541 cagttgatgc aacaacggcg caggtatggc tgcggcttcg tcgaccacca gccagtcggc
    60601 ttgctcatcg ctggctaaca aggcatccgg tgcaataaag cgaaacttct cgcccgcaaa
    60661 ttgtgccagt acatccgttg ccgcttttgc gggcgcggtg acaatcgcac tgcccgcaat
    60721 acgagaaatg agttgccctg ccaacgccga cttaccgcgc ccacgcgcag ccgttaccgc
    60781 tgccacgccc ggcggcatgg tcagtagctg ctgtaagagt tgctgttgtt ctggttgtgg
    60841 tgcgccggtc gcggggtgcc agtcagtacg gggagtaaaa tgcgccaacg agaacggctg
    60901 gttttgccgc cagaggatag cgtcgttatc cgccgtaagt acgcgtttga gatgctggac
    60961 aaaatgcggc gtcgcaatag ggtcagggca atcactccag cgcagcgagt cggcatcagg
    61021 ttggttttcc cactcttccc atacagggag taacaaaacc agccagcttc ccgctttcaa
    61081 cgttccgcta agtgctgcaa aggcagcggc atcaaagccg tggcgggcgt cgaataccgc
    61141 atgccggaac tcgcgcccaa gtaaagtttg tagcgccgag ggtgaacagt ggttttcagc
    61201 atctagctgc ggcgaaatcc acagccagtc gccaggtaag gcatcacgca acttaagagc
    61261 atgatcaaaa caccaaccct cttccccgct caacaccagc aagcggcaga tcccttcacg
    61321 tttcatttgc gctgttaatg tgtaaagcgc agtcagttca gccatccctg ccccggaaat
    61381 taaatgcttt taccaaaagt attgcattgt gccggatcgc cgctgtcgaa accacgttta
    61441 aaccagctgt agcgttgctg agaagtgcca tgtgtgaaac tgtctggtac tactcgcccc
    61501 tgactttgct gttgtaaacg gtcatcgccg atggcctgcg ccgcattcag cgcctcttcc
    61561 agatcgccag tttccagaac gccttgctgc tgcatactat gcccccagac accggcaaaa
    61621 cagtcggcct ggagttccat acgcacagat aagcggttta cttccgcctg cgtcgcgttt
    61681 tgttgcagtt gacgaacttt cggctcgatg cctaacagtt tctgcacatg atgaccgact
    61741 tcatgggcga taacgtaccc ctgggcaaaa tcgccatccg cgccaagttt gtctttcatg
    61801 tcatcataga aggagagatc gatataaact gtgccatccg ccgggcaata gaacggcccc
    61861 ataatggact ggcccgcccc gcagccggtg cgcgtcattc cacggtacat gaccagcttc
    61921 ggttgctgat aggtcttacc catcttttcg aactgttgtc cccaggtgtc ttccgtggtt
    61981 gcctgaatca ccgaggtgaa ttttgcggct tcgtcttcat tagggctaat tgaccgcgtt
    62041 gattgttgtt gagaaaccgg ctgcccggtc atcaacccgg ttaaatcaac gccatagtag
    62101 cctgcaacca gcacgactat cagtaaaatc agcccgcctt taccgctcgg cagacggaaa
    62161 ccgggaccgc ccattgacgg accgccagag ctgttgcgcc tgtcttcaac attgtcactt
    62221 tcacgtcgcc cttgccaacg catagatacc tcaacaatat attcattata gaaatgatcg
    62281 taggtggttc agaggaagat taccacagga aagcgggatg aaatggcgga ggaatgagcg
    62341 ttcaatgcat atattccaag agcggttgtc ttgccgtgcc agctgcacgg caagatgatg
    62401 aacagaaaaa tcagtccagc tgtacaccca ggcggcgggc gacggcttca taggcttcga
    62461 tcaggccacc gaggctctgg cggaaacggt ctttgtccat tttctccagt gtttctttgt
    62521 cccacaggcg gctgccgtcc ggggagaact catcgcccag taccacttca cctttgtaca
    62581 gaccaaactc cagcttgaag tcgaccagaa tcagaccagc atcatcgaac agttttttca
    62641 gcacgtcgtt cgctttgtag gtcagctctt tcatacgcgc caggttctct ttgctcaccc
    62701 agccaaaggt ttcgcagtaa gattcgttga ccatcggatc gtgcatggca tcgtttttca
    62761 ggaacagatc gaacagcggc gggttcagct caataccttc ttcgattcca agacgtttca
    62821 ctagagagcc agcagcacgg ttacgcacga cacactcaac cggcaccata tccagctttt
    62881 tcaccagaca ttcggtatcg gagagcagac gctccatttg agtcgggata cccgcttcag
    62941 ccagtttgct cataatgaag tagttgaact tgttgttcac catacctttg cgatcaaact
    63001 gctcaatgcg cgcgccatcc cctgctgacg tatcattgcg gaattcgagc accaacaggt
    63061 ccgggttttc cgtgctgtat acggttttcg ctttaccacg atacaactca gcttgctttt
    63121 gcatctttat cactcctggg tgtgaattaa cgttttaaaa tcttttgctg tctggtgtgc
    63181 cggatgtttt gtcggatgcg gcgtgaacgc cttatccgaa ctacgtccga caaacaaatt
    63241 tcgtgcgaat taccgctaaa atcgcgtttt cctgccgacg cacacgtttg cgtatcatat
    63301 cagaaaaaag ggccggatga ttccagccct gtatttttac ttgctaaacg cagcctggaa
    63361 gacagctacc agcgcgtcgt tctgactctg agtcagagta tgacctttcg gatcgatgaa
    63421 ctgtaggctg ctgcggttat ctaaatcgcc aacctgcagt ttatagtcac cggatgccag
    63481 gcctggatcg ctcgcgccca gttcctgcca gtcgctgtca gacagcggct tataagttac
    63541 ggccatgttg ccctgcgaac gggtgctgtc ggtcactttc atgcccactt tttccagcgc
    63601 cgctggcaga cgttgccaaa ccacattgaa cggcccgcgt acgaccagca ttggtaaacc
    63661 ggtgtcatca gctgcacttt gtacgtccat agtggtggag gcacgatttt gcgcagcgtt
    63721 cgcggcgtca gtggcagatt tatccagacc ggcggaaata acgttcatca tctccgtgct
    63781 gtaacgctgc atggaagccg cgtctgcaac cggtttgccc gcctgttcca ggttcagcag
    63841 tttaaccgta accgcctgct gataaccctg cggcttaaca gagatttgat aacgaccacg
    63901 atactgctcg tcttcgtcca gacggttcca ttgtacccaa tcggtggtca gtgtctgacc
    63961 agcatcatca cgttgggtga tggtgtagtt tttcgcctgc agcacgctaa ccacctgcgg
    64021 ccacagagta ttgccacgac cattttccac cagcaatgaa gcggtatcgc ccgtgaactg
    64081 ggtacgcgcg ccagaaacca gtgccaacgg ctgggctggt ggacgaatgt ccagcgcctt
    64141 accgacagca ccactaccgt tggtcaccgg gattgcataa tcaccggagg tcaccggcaa
    64201 aatcattcca gccggggcat gaagctccgc aagcggtgcc gcttccaggt aggcttcatc
    64261 accactgacc tgacgcttat agcgtgagtc agaactacag gcagcgagta ataaaacaag
    64321 cgaaacaccc gcaacctttg ccaggcgcga cttttgaaca gagtaagcca tcaaatctcc
    64381 ctaaacttta cagcaaaccg gcatgcttaa gcgccgctct gaccgtctca cgaccactgt
    64441 cggtgattgg tgtcattggc aggcgcagcg tatcggtcgc cacaagaccc agttccttac
    64501 atgcccattt caccgggatt ggattgggtt cgacaaatag tttgttgtgt aatggcatca
    64561 gacgctgatt aataacgcgt gcctcggcaa aatgcccttc tgctgccagt ttgcacatct
    64621 gggccatatc acgcgctgcg acgttagccg taacggaaat aactccatga ccgcccaatt
    64681 gcatgaagtc cagcgcgctc gcatcatcgc cgctcagcag aacaaaatca tctgaaacca
    64741 gctctttgat ctggtttaca cgcgttaagt tccctgttgc ctctttgatt ccgataatat
    64801 tttttacttt cgccagacgg cccaccgttt ccgggagcag atcgcagcca gtacgggacg
    64861 gcacattata cagaatttgc ggcaggtcag tatgctcagc gatggctttg aaatgctgat
    64921 acaaaccttc ttgcgacgga cgattgtagt aaggggttac cgtcaggcag ccgacgatac
    64981 cactgtcatt gaagcgctgc gtcaggctaa tggcttccgc agtagcgtta gcgccggtcc
    65041 cggcaattac cggaatgcgc ccatcagcca gatccagcgt catcatcacc acatcagcat
    65101 gttcgtcatg atttaaggta gcggactcgc cagtggtgcc aacagaaacg atcgccgaag
    65161 taccgctggc gacatgataa tcaatcagtt ttttcaagct agcccgacag acattacctt
    65221 tttcatccat cggagtaaca atcgcgacaa tacttcccgt gaacatgggc catcctctgt
    65281 gcaaacaagt gtctcaatgg tacgtttggt atggcattaa aagcaagcag acagaaccgt
    65341 tctgattgtt gtatgcatga tttttttatg ctttccttaa gaacaactca ccccttaaag
    65401 gaataaccag tttgacactg tcatcgcaac attatctggt gatcactgcg ttgggtgccg
    65461 atcgccctgg aattgtgaac accatcaccc gtcatgtcag tagttgcggc tgtaatattg
    65521 aagacagtcg cctggcgatg ctgggagaag agttcacgtt tatcatgctg ctttccggtt
    65581 catggaatgc catcactctg attgaatcaa cgttaccgtt gaaaggtgcc gaactggatc
    65641 ttttaatcgt gatgaagcgc acgacggcgc gtccgcgtcc gccaatgcca gcatctgtct
    65701 gggttcaggt cgatgttgca gactccccgc atttaattga acgcttcaca gcacttttcg
    65761 acgcgcatca tatgaacatt gcggagctgg tgtcgcgcac acaacctgct gaaaatgaac
    65821 gggctgcgca gttgcatatt cagataaccg cccacagccc cgcatctgcg gacgcagcaa
    65881 atattgagca agcgttcaaa gccctatgta cagaactcaa tgcacaaggc agtattaacg
    65941 tcgtcaatta ttcccaacat gatgaacagg atggagttaa gtaatgaatc cactgaaagc
    66001 cggtgatatc gcaccgaaat ttagcttgcc ggatcaagac ggagaacaag ttaatttgac
    66061 cgacttccag ggacagcgtg ttctggttta tttctacccg aaagccatga cccccggctg
    66121 taccgtacag gcctgcggct tacgcgataa catggatgag ttgaaaaaag cgggcgttga
    66181 tgtgctgggt atcagcaccg ataaacccga aaaactctcc cgttttgcgg aaaaagagct
    66241 gcttaacttt acgctcctgt ctgatgagga ccaccaggtg tgcgaacaat tcggcgtctg
    66301 gggtgaaaag tccttcatgg gcaaaaccta cgatggtatt catcgcatca gcttcctgat
    66361 tgacgctgat ggcaaaatcg aacatgtctt tgacgatttc aaaaccagca atcaccacga
    66421 cgttgtgctg aactggctga aagaacacgc ctgattactt tgctccattc cgtgctggct
    66481 gcgcttgcgg ccagcatacc tcacttctcg tgatcaagat cacattctcg ctttcccctg
    66541 cgacacgggt gtcgaatcca ttttttgctg aacgttaatg accatcattt ttgtaccgtt
    66601 cagaatccag ttaatacata acttattgaa tagattgagt taatcagaat ggcatccttt
    66661 atacaatatg aaatgcaatg tttcatatca ttttcaagga gccgacatga accgctttgt
    66721 ggtggccgaa ccactgtggt gtacaggatg taatacctgt ctcgctgcct gttcggacgt
    66781 gcataaaacg caaggtttac agcaacaccc gcgcctggcc ctggcgaaga cgtcaacaat
    66841 cactgcccct gtcgtgtgtc atcactgtga ggaagcccct tgcctgcagg tctgcccggt
    66901 caatgccatc tctcagaggg atgatgcgat ccaactcaac gaaagcctct gtattggctg
    66961 caagctttgc gccgtggtct gcccatttgg cgcaatcagc gcttcaggaa gccgtccggt
    67021 gaatgcccat gcgcaatatg tttttcaggc tgaaggctca ctcaaagacg gcgaagaaaa
    67081 cgtgccgccc caacatgcgt tgctgcgctg ggaaccgggt gtccagaccg tcgcggtgaa
    67141 atgcgacctg tgtgatttct tgccagaagg tccggcctgc gttcgcgctt gcccgaatca
    67201 ggcgttacgg ctgatcaccg acgatagcct gcaacgtcag atgaaagaaa aacagcgcct
    67261 tgccgcaagc tggtttgcca atggcgggga gtctcccctt tccctcactc aggagcaaca
    67321 ctaatggatg ccctgcaatt attaacctgg tcgctgatcc tctatctgtt tgccagtctg
    67381 gcttcgctgt ttttactcgg tctggacaga ctggctatta agctttccgg catcacatcg
    67441 ctggtgggcg gcgtgattgg catcatcagc ggaattacgc aattacatgc tggtgtaact
    67501 ttagtcgccc attttgccac cccttttgac tttgccgatt taaccctgcg aatggatagc
    67561 ctctcggcat ttatggtgct agttatctcc ttgctggtgg tggtttgttc gctctattca
    67621 ttgacttata tgcgcgaata cgagggcaaa ggcgcggcgg cgatgggctt ctttatgaat
    67681 cttttcatcg catcgatggt tgccctgctg gtgatggaca acgctttttg gttcatcgtg
    67741 ctgtttgaaa tgatgtcgct gtcttcctgg tttctggtca ttgccaggca ggataaaacg
    67801 tcgatcaacg ctggcatgct ctactttttt atcgcccacg ccggatcggt gctgattatg
    67861 atcgccttct tgctgatggg gcgcgaaagc ggcagcctcg attttgccag tttcgcacgc
    67921 tttcactttc tccggggctg gcgtcggcgg tgtttctgct ggcctttttc ggttttggcg
    67981 cgaaagccgg gatgatgccg ttgcacagct ggttgccgcg cgctcaccct gccgcaccat
    68041 cgcacgcttc ggcgttgatg tctggcgtaa tggtcaaaat cggtattttc ggcatcctga
    68101 aagtagcgat ggatctgctg gcgcaaacgg gtttgcctct gtggtggggc attctggtga
    68161 tggcgatcgg cgcaatctcc gcgctcctgg gcgtgctata tgcgctggcg gaacaggata
    68221 tcaaacggct gctggcctgg agtaccgtcg aaaacgtcgg cattattttg ctggcagtcg
    68281 gtgtggcgat ggacggtctg tcactgcacg acccgctgct caccgtggtt ggactgctcg
    68341 gcgcactgtt tcatctgctc aaccatgcgc tgttcaaagg gctgctattt ctcggcgcgg
    68401 acgcgattat ttcgcgtttg catacccacg acatggaaaa aatgggggca ctggcgaaac
    68461 ggatgccgtg gacagccgca gcatgcctga ttggttgcct cgcgatatca gccattcctc
    68521 cgctgaatgg ttttatcagc gaatggtaca cctggcagtc gctgttctca ctaagtcgtg
    68581 tggaagccgt agcgctacaa cttgcgggtc ctattgctat ggtaatgctg gcagtcactg
    68641 gtgggctggc agtaatgtgc ttcgtcaaaa tgtacggtat tactttctgt ggtgcgccgc
    68701 gcagtacaca cgctgaagag gcacaggaag tgccaaatac gatgatcgtc gccatgctac
    68761 tgctcgcggc actctgcgta ttaattgcgc ttagtgccag ttggctggca ccgaagataa
    68821 tgcatattgc ccatgcgttt accaataccc ctcccgtcac tgtcgccagc ggaatagcac
    68881 ttgtacccgg cacgtttcat acacgggtca ctccttcatt actgttgctg ttactactgg
    68941 cgatgccttt gctgccaggc ctttactggc tgtggtgtcg ttcgcgccgc gcagcgtttc
    69001 gtcgcacagg agatgcctgg gcatgcggct acggctggga aaacgcgatg gctccgtcag
    69061 gcaatggcgt gatgcagccg ctgcgtgtgg tcttctctgc gttatttcgt ctacgacaac
    69121 agctcgaccc tacgctgagg ctaaataaag gtcttgcgca cgtcaccgcc agggctcaga
    69181 gcacagaacc cttctgggat gagcgggtga tccgccccat cgtgagcgcc acccaacggc
    69241 tggtcaaaga aatacagcat ctgcaaagcg gcgactttcg tctctattgc ctgtatgtgg
    69301 tcgccgcact ggttgtgctg ctaatcgcta ttgccgtcta aggaaatcac catgagacaa
    69361 actctttgcg acggatatct ggtcattttt gcgttagcac aggccgtgat tctgctgatg
    69421 ctaaccccac tttttacggg tatttcccgg cagatacgcg cgcgtatgca ctcccgccgc
    69481 gggccgggga tctggcagga ttatcgcgat atccacaaac tgtttaaacg ccaggaagtt
    69541 gcgccgacat cttcaggtct gatgttccgc ctgatgccgt gggtattaat cagcagcatg
    69601 ctggtgctgg cgatggcctt accactattt attaccgttt ccccttttgc gggcggcggc
    69661 gatctgatca cccttatcta tcttcttgcc ctgtttcgtt ttttctttgc tctttccggg
    69721 ctggataccg gaagtccgtt tgcgggagtc ggtgccagtc gcgagttgac gctcggcatt
    69781 ctggtcgaac caatgcttat tctctcactg ctggtattgg cgctgatagc aggttccacg
    69841 catatcgaga tgatcagcaa tacgctggct atgggctgga actcgccgct aaccaccgta
    69901 ctggcgttac tggcctgtgg ttttgcctgc ttcattgaga tgggaaaaat tccctttgat
    69961 gttgctgaag cagaacagga attacaggaa ggcccgctga ccgaatattc cggtgccggg
    70021 ctggcgctag cgaaatgggg gctggggctg aaacaggtcg tgatggcagc actgtttgtg
    70081 gccctgtttc tgccctttgg gcgcgcgcaa gaactttctc tcgcctgcct gctgacttca
    70141 cttgtcgtta cgctgctcaa ggttttgctg atttttgtac tggcctcaat cgcagaaaac
    70201 acgctggcac gcgggcgttt tttactcatt caccatgtga cctggcttgg cttcagcctt
    70261 gctgcgcttg catgggtctt ttggttaacc ggtctgtaag gagcactgac ggaatatgga
    70321 aaatcttgct ctgacgacgt taatgctgcc ttttatcggc gcactggtcg tttcgttttc
    70381 gccacaacgt cgggccgccg aatggggggt tttgttcgcc gcgctgacca cgctgtgcat
    70441 gttgtcgctg atctccgcgt tttatcaggc cgataaagtt gccgtcacgt tgacgttggt
    70501 caacgtgggg gatgtggcat tatttggcct ggtcattgat cgcgtgagta cgctgattct
    70561 gtttgtggtg gtgtttctcg gtttgctggt cacgatctac tccacgggtt atctgacgga
    70621 taaaaatcgc gaacacccgc ataacggcac gaatcgttat tacgcatttt tgctggtgtt
    70681 tatcggcgcg atggcgggac tggtactctc ctcgacgctg ctcggtcagt tgttgttttt
    70741 tgaaattacg ggcggctgct cctgggcgtt gatcagttat taccagagcg ataaagcgca
    70801 gcgttcagca ctaaaagcgt tgcttatcac ccatatcggt tcgttggggt tgtatcttgc
    70861 cgccgccacg ctgtttttgc agaccggaac gtttgtgctt agcgcgatga gcgagttaca
    70921 tggcgacgca cgttatctgg tttatggcgg catcctgttt gccgcgtggg ggaaatcggc
    70981 ccagctaccg atgcaagcgt ggctaccgga cgcaatggaa gcgccaacac cgatcagcgc
    71041 ctatctccac gccgcatcga tggtgaaagt gggcgtttac atttttgccc gcgccattat
    71101 cgacggcggc aatatcccgc atgtgattgg cggcgttggc atggtcatga cactggtcac
    71161 cattctttat ggctttctga tgtatttgcc acagcaggat atgaagcggt tgctggcctg
    71221 gtcgaccatc actcaacttg gctggatgtt cttcggcttg tcgctctcca tcttcggctc
    71281 gcggctggcg ctggagggta gcatcgccta catcgtcaac cacgcgttcg ctaaaagcct
    71341 gtttttcctt gtagcaggtg cgctgagtta cagctgcggc acgcgcttgt tgccgcgtct
    71401 gcgtggcgta ttgcacaccc tgccgttgcc aggcgtgggt ttctgcgtgg cagcgctggc
    71461 aattaccggt gtgccgccgt tcaacggctt cttcagtaaa ttcccgctgt ttgccgccgg
    71521 ttttgcgttg tcagtggagt actggatcct gctgcccgcc atgattctgc tgatgattga
    71581 atcggtcgcc agtttcgcct ggtttattcg ctggtttggt cgcgtcgtgc cgggcaaacc
    71641 gagcgaggcc gtcgccgatg ccgcaccgct gccagggtca atgcgcctgg tgttgattgt
    71701 actgattgtg atgtcgctga tttccagcgt aatcgccgcg acctggttgc agtaaggaga
    71761 tgatgaatga ccggttctat gatcgtaaat aatctggcgg gactgatgat gctgacatcg
    71821 ctgtttgtga ttagcgtcaa aagctatcgc ctgtcatgcg gattttacgc ctgccagtcg
    71881 ctggtgctgg tgtctatttt cgccactctc tcgtgcctgt tcgccacaga gcaactgctg
    71941 atctggtccg ccagcgcctt tatcaccaaa gtgctgctgg taccgttaat catgacttac
    72001 gctgcacgaa atattcccca gaacatcccg gaaaaagcgt tattcggtcc ggcaatgatg
    72061 gcactgctcg cggcgttaat tgtcctgctt tgcgcatttg tcgttcagcc cgtgaagcta
    72121 ccgatggcta ccgggctgaa accggcgctg gcggtagcgt taggtcattt tctgcttggc
    72181 ctgctgtgca ttgtcagcca acgcaatatc ctgcggcaaa tttttggtta ctgcctgatg
    72241 gaaaacggct cccatctggt gctggcgctt cttgcctggc gagcaccgga actggtggaa
    72301 ataggtatcg ctaccgacgc catcttcgcc gtcattgtga tggtgttact ggcaagaaaa
    72361 atatggcgta cccacggcac gctggacgtg aacaacttga ccgcgctgaa gggataatga
    72421 gatgagttat tctgtgatgt tcgctttact cctgctcacg ccgctgcttt tttcgctgct
    72481 ctgttttgcc tgccggaaac gggggctctc tgcgacttgt acggtgactg tattacatag
    72541 cttagggatc acactgctgc tgattctagc actctgggtg gtccaaactg ccgctgatgc
    72601 aggagaaata ttcgctgcgg gactgtggct tcatattgat ggtctgggcg gtttgttcct
    72661 cgccattctt ggtgtgattg gctttctcac cggtgtttac tcgattggct acatgcgtca
    72721 tgaagtggca cacggcgagc tttcacccgt tacactgtgc gattactacg gtttcttcca
    72781 tctgtttttg ttcaccatgc tgctggttgt taccagcaat aacctgattg tgatgtgggc
    72841 ggcgatcgaa gccaccacct taagctcggc gtttctggta ggcatttacg gtcagcgttc
    72901 atcgctggaa tcagcatgga agtacatcat tatttgtact gttggtgtcg cttttggtct
    72961 gttcggtacc gtgctggtat acgccaacgc cgccagcgtt atgccgcagg cagaaatggc
    73021 gatattctgg agcgaggttc ttaagcaatc gtccttgctt gacccaacat taatgctgtt
    73081 ggcctttgtg tttgtgctaa ttggctttgg taccaaaacc gggctatttc ccatgcacgc
    73141 ctggctgccg gatgctcaca gtgaagcgcc gagtccggtc agcgccctgc tctccgccgt
    73201 attgctgaac tgcgcgctgt tggtgctgat tcgctattac atcattattt gccaggccat
    73261 cggcagcgat ttccccaacc ggttgttgct catcttcggc atgttgtcgg ttgccgtggc
    73321 ggcatttttc attctggtac agcgggacat taagcgtctg ctggcgtact ccagcgtgga
    73381 gaacatgggg ctggtcgcgg tggcgctagg cattggcggg ccgctgggaa tttttgccgc
    73441 gctgctgcac accttaaacc acagtctggc aaaaacgctg ctgttctgcg gttccggcaa
    73501 tgtactgctc aagtacggca cgcgcgatct caacgtcgtc tgtgggatgc tcaaaatcat
    73561 gccatttacc gccgtgctgt ttggcggcgg tgcgctggcg ctggcgctgg cgctggcgct
    73621 ggcagggatg ccgcccttca acatttttct tagcgaattt atgaccgtta ccgcaggact
    73681 ggcacgtaat cacctgctga ttatcgtcct gctgttattg ctgttaacgc tggtgctggc
    73741 gggcctggta cggatggctg cgcgggtgtt aatggcgaaa ccgccgcagg ccgttaaccg
    73801 gggtgatctc ggctggttga ccacctcgcc aatggtgatt ctgctggtca tgatgctggc
    73861 gatgggaacg catattccac aacctgtcat caggatcctg gcgggcgctt ccactatagt
    73921 cctctcaggg acgcacgacc tgcctgcaca acgtagcacc tggcatgatt ttttgccttc
    73981 aggcaccgca tctgtttcgg agaaacacag tgaacgttaa ttcatcgtca aatcgtggcg
    74041 aagcgattct cgccgccctg aaaacgcagt tccccggcgc ggtgctggat gaagagcgac
    74101 aaacgcctga acaggtcacc attacggtga aaatcaatct gctgcctgac gttgtacatt
    74161 atctttatta tcaacatgat ggctggcttc cagtcctgtt tggcaacgac gagcggacac
    74221 ttaacggtca ttacgcggtt tattatgccc tttcaatgga aggggccgaa aaatgctgga
    74281 tcgtggtgaa ggcactggtc gatgccgaca gtcgggagtt tccgtcagtc acaccgcgcg
    74341 tccctgccgc ggtctggggc gagcgagaaa ttcgcgatat gtacgggctg attccggttg
    74401 gcctgccgga tcagcgtcgc ctggtgttgc ccgatgactg gccggaagat atgcatccgc
    74461 tgcgcaaaga tgcgatggat tatcgactgc gccctgaacc gacgacagat tccgaaacgt
    74521 atgcgtttat caatgagggc aacagcgatg cgcgggtgat ccctgtcggc ccgctgcata
    74581 tcacctccta tgaaccgggt cacttccgct tgtttgtgga tggcgagcaa attgtcgatg
    74641 ctgattaccg cctgttttat gtccatcgcg gcatggagaa actggcagaa acgcggatgg
    74701 gctacaacga agtgaccttc ttatcggacc gcgtgtgtgg gatttgcggt tttgcccaca
    74761 gtgtggccta taccaactcg gttgaaaatg cactggggat tgaggtgccg caacgagcac
    74821 atactattcg ctcgattctg ctggaagtcg agcggctaca cagtcatttg ctcaaccttg
    74881 gcctctcctg ccattttgtt ggttttgata ccggctttat gcaatttttc cgcgtgcggg
    74941 aaaagtcgat gacgatggcg gaattgctga ccgggtcgcg taaaacctac ggtctgaatc
    75001 tgattggtgg tgttcgccgc gatattctca aagagcaacg tctgcaaacg ctgaaactgg
    75061 tgcgcgagat gcgcgccgac gtgtcggagc tggtagagat gctgcttgct acgccgaata
    75121 tggaacaacg cactcagggc attggcattc tcgaccgaca aatcgcccgt gattatagcc
    75181 ctgtagggcc gctgatccgc gcagtggttt tgcccgtgat ttgcgctttg atcaccccta
    75241 cgccgactac ggcaatattc caaaaacact gtttaccttt accggcggcg atgttttctc
    75301 ccgcgtgatg gtccgtgtca aagagacgtt tgattcgctg gcaatgctgg aatttgccct
    75361 cgacaacatg ccggataccc cactgctgac cgaaggcttt agctataaac ctcacgcatt
    75421 cgcgctgggc tttgttgaag cgccacgcgg tgaagacgtg cactggagca tgctcggtga
    75481 taaccaaaaa ttgttccgct ggcgctgtcg tgccgccacc tacgccaact ggccggtgtt
    75541 gcgttacatg ctgcgcggca ataccgtttc tgacgcaccg ctgattatcg gtagccttga
    75601 tccctgctac tcctgtaccg accgtgtgac gctggtagat gtgcgcaagc gccagtcaaa
    75661 aaccgtgctg tataaagaga tcgaacgcta cggcattgat cgtaaccgtt cgccgctgaa
    75721 gtaaggacag aagatgctga agttactgaa aactattatg cgcgccggaa ccgcgacggt
    75781 gaaatatccc ttcgcgccac tggaggtcag cccaggcttt cgcgggaaac cggacctgat
    75841 gcccagccaa tgtattgcct gcggtgcctg cgcctgtgct tgtccggcaa atgcgctgac
    75901 tatccagacc gacgaccagc aaaattcgcg cacctggcag ctctatctgg ggcgttgtat
    75961 ttactgcgga cgttgtgaag aagtgtgccc gaccagagcc atccagctta ccaataactt
    76021 tgaactgacc gtcacccata aagccgatct ctatacccac gcgacgttcc atctacaacg
    76081 ttgcagccgt tgcgaacgcc cgtttgcccc gcaaaaaacc gtcgcactgg ctgctgaatt
    76141 gttggcacag caacaaaatg ctccacaaaa ccgcgaaatg ttatgggcgc aagcgagcgt
    76201 ctgcccggag tgcaaacaac gcgcaacgct gatcaacgac gatacagatg taccgctggt
    76261 ggctaaggag cagctatgag tccagtgctt acgcaatatg tcagccagcc catcacgctg
    76321 gacgagcaaa cgcaaaagat gaagcggcat ttgctacagg atatccgtcg ctcggcttac
    76381 gtttatcgca tcgattgcgg cggctgcaac gcctgtgaaa tcgaaatttt tgctgccatc
    76441 acccccgtat tcgacgcaga acgttttggc attaaggttg tttcatcacc gcgtcacgcc
    76501 gatattttgt tatttactgg cgcagtcacc cgggcgatgc gtatgcctgc acttcgggcg
    76561 tatgagtctg cccccgatca taaaatctgt gtttcctacg gcgcgtgcgg tgtcggcggc
    76621 ggtattttcc acgatctcta cagcgtctgg ggcggcagcg acaccattgt ccccattgat
    76681 gtttggatcc ccggctgccc gccaacaccg gccgccacca ttcacggttt cgccgtggcg
    76741 ctcggtttgc tgcaacagaa gattcacgcg gtggattatc gcgatcccac cggggtgact
    76801 atgcaaccgt tgtggccgca gatcccgcca tcacagcgta tcgccattga gcgagaagcg
    76861 cggcggctgg cgggctatcg tcaggggcga gaaatttgcg atcggctcct gcgccattta
    76921 agcgacgatc ctacaggaaa tcgggttaac acctggttgc gcgatgccga cgatccacgt
    76981 ctcaatagta tcgttcagca actctttcgc gtactccggg ggttacatga ctgaagagtg
    77041 cggggaaatt gttttctgga cgctgcgaaa aaagtttgtc gccagtagcg acgagatgcc
    77101 ggaacacagc tctcaggtaa tgtattactc gctggctatc ggtcatcacg ttggcgtgat
    77161 tgattgtctg aatgtcgcct tccgctgccc actgacggaa tacgaagatt ggcttgcact
    77221 ggtcgaagag gagcaagccc gacgtaagat gctgggggtg atgacttttg gtgagattgt
    77281 tattgacgcc agccacaccg ccctgttgac ccgggcattc gcgccactgg cagatgacgc
    77341 gacgtctgtg tggcaagcgc gtagcattca attcattcat ctgttggatg aaattgtgct
    77401 ggaaccggct atctatctga tggccagaaa aattgcgtga gaaggatttc tcattaataa
    77461 ggactgttga tggctatgtc agacgaagcg atgtttgccc cgccgcaagg aataacaatt
    77521 gaagcggtaa acggaatgct cgcggagcgg ttagcgcaga aacacggcaa ggcgtcttta
    77581 ttacgcgcct tcatcccgct gccgccgccg ttcagcccgg tacaacttat tgaactgcat
    77641 gttctcaaaa gcaacttcta ttaccgctac catgatgatg gcagcgatgt gacggcaaca
    77701 acagagtatc agggcgagat ggtcgattat tcgcgttacg ccgtccttct cggcagtagt
    77761 ggaatggcgg agctacgctt tattcgtacc cacggcagcc gttttactcc ccaggattgc
    77821 acactgttta actggctggc acgcattatc accccggttc tgcaatcatg gctcaatgat
    77881 gaagcacagc aggtggcgct gcgtttgctg gagaaagatc gcgatcatca tcgggtactg
    77941 gtggatatca ctaatgcagt gctgtcacat cttgatctcg acgatctgat cgctgacgtc
    78001 gctcgtgaga tccatcattt tttcggtctg gcttcagtca gtatggtact gggcgatcat
    78061 cgaaagaacg agaagttcag cctgtggtgc agcgatcttt ctgcctcaca ttgtgcgtgt
    78121 ctgccacaca atatgcctgg cgacagtgta ttgctgacac aaacgctaca aacccgacaa
    78181 ccgaccttga cgcaccgtgc agatgatctg tttctctggc aacgcgaccc gttattactc
    78241 ttacttgcat ctaacggctg cgaatctgcg ctccttatac cgcttacctt tggcaaccat
    78301 acaccgggtg cattgttgct ggcgcatacc tcttccactc tctttagtga ggaaaactgc
    78361 cagctactac aacacatagc cgatcgcatc gctattgccg ttggcaatgc cgatgcctgc
    78421 cgtagaatga ccgatttgca ggaaagtttg cagcaagaaa accaccagct tagcgagcag
    78481 ctcctttcga atctgggcgt cggtgacatt atctatcaaa gccaggcaat ggaagacctg
    78541 ctccagcagg tagatattgt ggcgaagagc gacagtacgg tgttgatttg cggtgaaacc
    78601 ggaaccggca aagaggtgat cgccagagcg atccatcaac ttagcccgcg acacgacaag
    78661 ccgctggtca aaatcaactg cgctgccatc cccgccagtc ttctggaaag tgagttattc
    78721 ggtcatgaca aaggggcgtt tactggtgcg attaataccc atcgtggtcg ttttgaaatt
    78781 gccgatggcg gcacgttgtt tctcgatgaa attggcgatc tgccgttaga acttcagcct
    78841 aaactgctgc gcgtattgca ggaacgggag attgagcgtc tcggcgggag tagaacgatc
    78901 ccggtaaatg tcagagtcat tgccgccacc aaccgtgatt tgtggcaaat ggttgaagat
    78961 cgccagtttc gcagcgatct cttttatcgc ctgaatgtct tcccactgga attgcctccg
    79021 ctgcgcgacc gtccggaaga tatccctctt ttagcaaagc atttcacgca aaaaatggcg
    79081 cgccatatga atcgttcaat tgacgccatc ccgaccgagg cgctacgcca gttgatgtcg
    79141 tgggactggc cgggcaacgt gcgcgagctg gaaaacgtga ttgagcgagc ggtactgctg
    79201 actcgcggta acagtctgaa tttacatctt aatgtccgac aaagccgttt actgccgacg
    79261 ctaaatgaag attcagcgct tcgcagttca atggcgtagt tgctgcaccc gacgacgcca
    79321 gagaatgacg aagaagaacg tcagcgcatt gttcaggtat tgcgagaaac caatggcatt
    79381 gttgccgggc cccgtggcgc ggcgacacga ttagggatga agcgcaccac gctgctgtca
    79441 cgaatgcagc gtctgggaat ctcggttcgc gaggtgttgt aatctgcttt tgcaggagta
    79501 tgcatgagaa acaaactctc tttcgacttg cagttgagcg ccagaaaagc ggcaatcgct
    79561 gaacggattg ccgcccataa aattgcccgc agtaaagtgt cggtcttttt aatggcgatg
    79621 tccgctggcg tgtttatggc gatgtccgct ggcgtgttta tggcgatcgg atttactttt
    79681 tacctttccg ttatcgccga tgccccgtct tcataggcat taacccatct ggtgggcggc
    79741 ctttgcttta cacttggctt tattttgctg gcggtttgcg gcaccagcct gttcacctcg
    79801 tcggtaatga cggtaatggc aaaaagtcgg ggcgttatta gttggcgaac ttggctgatt
    79861 aacgcacttc tggtggcctg cggtaatctg gcaggtattg cctgtttcag tttgttaatc
    79921 tggttttccg ggctggtgat gagtgaaaac gcgatgtggg gagtcgcgat tttacactgc
    79981 gccgagggca aaatgcatca tacatttact gaatctgtca gcctcggcat tatgtgcaat
    80041 ctgatggttt gcctggcgct gtggatgagt tattgcggac gttcgttatg cgacaaaatc
    80101 gtcgccatga ttttgtccat caccctgttt gtcgccagtg gctttgagca ctgtatcgcc
    80161 aatttgtttg tgattccgtt cgccattgcc attcgccatt tcgcccctac ctccttctgg
    80221 caactggcgc acagtagcgc agacaatttt ccggcactga cggtcagcca ttttattacc
    80281 gccaatctgc tcccggtgat gctgggtaat attatcggcg gtgcagtgct ggtgagtatt
    80341 tgttatcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc gaaacagttc
    80401 gtcgggctat ttatttacgt caggaatcct gaaaaatcag cccggcgaaa cagttcgtcg
    80461 ggctgaagat tattcttgcg cgatttgccc atcgggccag gcgtggatca cggctttgat
    80521 cagcgtcgcc aatggaatgg cgaagaatac gccccagaat ccccacaaac caccgaagat
    80581 caccaccgat aaaataatca ccagcggatg caggttaacc gcttcggaga acaacaccgg
    80641 tactagcagg ttgccgtcca gcgcctgaat aatcagatac accgcgaagc agctccagaa
    80701 ttcagtacct gcgccaaact ggaatagcgc cacgccaacc accggaatgg tcaccacaaa
    80761 tgcgccaatg tacggaataa gaaccgagaa gccgaccagc actgccagca gcagcgaata
    80821 gttcagcccg aagagcaaga accccagcca ggtggcgatc cccaccacga tcatctccag
    80881 cactttgccg cggatatagt tggtgatttg ttgattcatc tccttccaca cctgtcctgc
    80941 cagcccacgg ttacgcggca gcacccggcg aacggcgttc agcatctgct ctttgtcttt
    81001 cagcaggaag aagaccatca atggcaccag caccagatag acggctatgg tcagcaaacc
    81061 gaccagcgag gcgagggaaa ttttcaccac cgaatcgccc atggtcagca tccgactgcg
    81121 catattttcg gccattgcat caataatgcc cgcgtccatt aacaccggat agcggcgcgg
    81181 caacgtggcg gcaaagtcag aaagcttatt gagcatcccc ggcatatcgc ggattaagta
    81241 gatgccctgt tgccaggcga taggcagtac cacgaacgcc atcagtagca atataccgac
    81301 aaaaaccacc aatacaatcg acgtcgccca gcggcgggag cagccaatag attgcaggcg
    81361 cacggttggc cattccagca aataagccag cacaatagcc accagcaacg gagcaagcag
    81421 gccactaaag aaaaagataa tgccaaatcc ggcaactaaa ataaccagca aggcaatcgc
    81481 ttccgggtcg ctaaaacggc ggcgatacca ttgcatcaac atttcgagca taaagacctt
    81541 ccctgaacct caagagcggg attgcgatcc gcaattgtat cgaaatgtca caaaaaagac
    81601 ttcgcttttt atgacggatt caggaaactg aaaagtcatt tgagtgggct aatcttcgcc
    81661 gttacactca aaggcggcga ggtgggaacg atatttcaca gtatcggtca aatgactatc
    81721 ttcagaaata caggatagag gttatgttca ggcagttgaa aaaaaacctg gttgcaaccc
    81781 tcattgctgc tatgaccatt ggtcaggtag ccccggcgtt tgccgacagc gcagacacct
    81841 tgccggatat gggaacctcc gcaggaagca cgctttccat tggtcaggaa atgcagatgg
    81901 gcgactatta tgtccgccag ctacgcggca gcgcgccgtt aattaatgac ccgctgttaa
    81961 cgcaatatat taattcgctg gggatgcgtc tggtttcgca tgccaattcg gttaagacac
    82021 cgtttcattt ttttctgatc aacaacgacg aaattaacgc ctttgctttc tttggcggca
    82081 acgtggtgct gcactctgcc ctgttccgtt attccgataa cgaaagtcaa ctggcttcag
    82141 ttatggcgca cgaaatctcc cacgtcaccc aacgtcacct ggcgcgagcg atggaagatc
    82201 aacaacgcag cgcgccgctg acctgggtcg gcgcgttagg ttctatttta ctggcgatgg
    82261 ccagtccgca ggcggggatg gcggcgctga ccggtacact ggcgggaacg cgccagggga
    82321 tgatcagttt cacccagcaa aatgaacagg aagcggaccg cattggtatt caggtgctgc
    82381 aacgctcggg attcgatcca caggcgatgc cgaccttcct ggaaaaattg ctcgatcagg
    82441 cgcgttactc ctcgcgcccg ccggaaattt tattgactca cccgttgccg gaaagtcgtc
    82501 tggcagatgc ccgcaaccgt gctaatcaga tgcgcccgat ggtggtgcag tcgtcggaag
    82561 atttctatct ggcgaaagcg cgcacactgg ggatgtataa ttccggacgt aaccagctca
    82621 ccagtgattt gctggatgaa tgggcgaaag gaaacgttcg tcagcaacga gcggcgcaat
    82681 atggtcgtgc tttacaggcg atggaagcca ataaatacga cgaggcgcgt aaaacgctgc
    82741 aaccgttact ggcggcagaa cctggcaacg catggtatct cgatctggct accgatatcg
    82801 atcttgggca aaacaaagcc aatgaggcaa tcaatcgcct gaaaaatgcc cgcgatttac
    82861 gcaccaatcc ggtgttgcag ctcaacctgg cgaacgctta tctgcaaggc ggtcaaccac
    82921 aagaagcggc caatattctg aatcgctaca cctttaataa taaagatgac agcaacggct
    82981 gggatttgct ggcacaggcg gaagccgcgc taaataaccg cgatcaggag ctggctgcgc
    83041 gagcagaagg ttatgcgctc gccggacgac tcgatcaggc catttcgctg ttgagtagcg
    83101 ccagttcgca ggtgaaatta ggcagcctgc aacaagcgcg ttacgatgcg cgcatcgacc
    83161 agttgcgcca gctgcagaaa cgctttaagc cttataccaa gatgtaattc gcattaaagg
    83221 aagaacaatg accaaacagg taaaaatcta ccataaccca cgctgttcaa agagccggga
    83281 aacgctgaac ctgctgaaag aaaacggcgt ggagccagaa gtggtgctct accttgagac
    83341 acccgccgat gcggcaacgc tgcgcgattt gctgaaaatg ctggggatga atagcgcccg
    83401 tgaattgatg cgccagaaag aggatcttta taaagaacta aacctggctg acagttcgct
    83461 tagcgaagag gcgctgattc aggcaatggt ggataatccg aagctgatgg agcgcccgat
    83521 tatggtcgcg aatggcaaag cgcggattgg tcgtccaccg gagcaggtac tggaaatcgt
    83581 gggttaagat gtgctccatc gctagttgaa gcacattgcc ggatgcgacg cttgccacgt
    83641 cttatccggc ctacgaatac tatggattaa cttcgcgtag gtcaggcgtt cgcgccgcat
    83701 ccgacaataa acaccttatc tacaacttca gaatttcttt cacaaacgga atggtcagct
    83761 tacgttgcgc ggtaatcgac gcacgatcca actgatccaa cgtcataaat agcgtgcgca
    83821 tttctctgtc gagccgcttc agcaagaaac gccccacatc ttccggcagt tcaaaaccac
    83881 gcaaacgcgc gcgtaactgt agcgcctgca acttatcttc atcagaaagt ggctgcaatt
    83941 tgtagatctg cccccagtcg agacgcgacg cgagatccgg taatcccaga ttcaactgcc
    84001 gcggtggacg atcgccggtg atcaacaacc gtgttttgcc cgattccaga attcgattgt
    84061 agagatcgaa aatcgccatc tcccacaact catcgcctgc aatacactca atgttgtcga
    84121 tacagaccag cgacaaatgc tccataccgt cgagcacttc cggaacaaac caggtgcgtt
    84181 tatccagcgg gacatagccc accgcatcgc cacgctgcga caattccgcg caagccgcgt
    84241 gcagcagatg gctgcgcccc gcgccttcgc gtgcccagag atagatgtaa ccgctatgtt
    84301 cctgacgcag cacgttttgc agcgcggcca gtaaagagga gttatccccc ggccagaaac
    84361 ttgcaaaggt ttcgtcgtca ggaagataaa gtggcaaaga gagctgtgcc ggtgtgttca
    84421 gagatacctc aaccaggatt tcacaaaatc gcgagaagtt taccacagaa tcccatgatg
    84481 tttgaaccgg gcagcaacac tgcccggtcg gtacattatt tgtctgttat atccgcgtct
    84541 tctgcgtcca gcaccacttc ttcagggcgc agcacactga tcagtttgaa aatcaaactt
    84601 aagccgatac cgacgatggt cgccagcgcc atacctttta actccgccgc gccgatgttt
    84661 accttcgcgc cactgacgcc gatgatcaaa atcacggaag tcaggatcag gttctgtgct
    84721 ttgttgtaat ccactttcga ttcgatcaaa acacgaatac cggaagcacc aatgacacca
    84781 taaagcagca gcgaaacgcc gcccatcacc ggcaatggga tcatctggat agcggcagcc
    84841 agtttaccga cgcaggaaag caggatagcg aaaatcgccg ccccgccgat aacccaggta
    84901 ctgtaaacac gggtgatcgc catcacgcca atgttttctc cgtaagtggt atttggcgta
    84961 gagccaaaga agccggagat cacggtcgac aggccgttag caaacatcga acggtgcaga
    85021 cctggatcgc gcagcagatc ttttttgacg atattagccg ttactaccag gtgccctacg
    85081 tgttcggcaa taaccactaa cgccgctggc agaatagtca gaatggcaaa ccactcgaag
    85141 cgcggcgtat agagggttgg cagtgcaaac cagtgagcat taataatcgg cgtggtatcg
    85201 acaattccca ttgcgaaaga gagcgcgtac cccaccagca cgccaattaa aatcgggata
    85261 attgccagga aaccacgaaa cagcacggaa cctaaaaccg tgaccgccag ggtggtaata
    85321 gagatgatga tggttttgga gtttggcgtt tgcccttcag ccgggagtaa acccgccata
    85381 ccggcagcta cgcccgccag ctccagaccg atgacggcaa cgattgcgcc cattgccgca
    85441 ggtggaaaca gcacgtccaa ccagccggtc cccgctttct tcacgataaa agaaaccagg
    85501 cagaacagca cgccgcacat aataaagccg cccagcgcga cttcataccc taacggcaac
    85561 agtaacaata ccggtgaaat aaaggcaaag ctggaaccaa gataagccgg aattttccct
    85621 ttacagatga agagatacag cagcgttcca ataccgttaa ataacagtac agtcgccggg
    85681 ttaatatgaa ataagacggg caccaggacg gttgcaccaa acatggcgaa caaatgttgc
    85741 aaactaagcg ggattgtctg taaaagtggc ggtctttcac tcaccccgat agcacggcgc
    85801 gtcatagtat tatcctctgt attatgtgtt ataggcgctt tactcaaaaa aaagccgact
    85861 cttaaagtcg gctttaatta tttttattct ttatttcgta ccaaagattt tgtcaccggc
    85921 atcgccgagg cccggaataa tgtatccgtg ctcgttcagt ccctggtcaa tcgatgcagt
    85981 atacagttcg acgtccgggt gcgctttttc cagcgcagcg ataccttctg gcgcagcgac
    86041 cagcaccaga actttgatgc tgctgcagcc cgcttttttc agcaggtcga tggtcgcgat
    86101 aacggaacca ccggttgcca gcattgggtc aacgatcagc gccatacgct catcgatgtt
    86161 agaaaccagt ttctggaagt acggtaccgg ctccagcgtt tcttcattac ggtacatacc
    86221 gacaacgctg atgcgcgcgc tcggaacgtt ttccagcaca ccgtccatca taccaagacc
    86281 cgcacgcaga attggcacaa cggtaatttt cttacctttg atctggtcga tttctaccgg
    86341 gccgttccag ccttcgatag ttactttttc cgtttcgagg tcggcggtcg cttcgtaagt
    86401 cagcaggcta cccacttcgg aagcgagttc gcgaaagcgc ttggtgctga tatcttgctc
    86461 acgcatcagt cccagcttgt gtttgacgag tgggtgtttg acttccacga tcttcatact
    86521 ctttctcctt tgaggggcag ccacaaaaaa atcgacggat tatacctcct ttcttcaggg
    86581 cggcaatatt attttcgttg actttagtca aaatgataac agtttgagat aaagttattt
    86641 tatattcaaa tggttatgaa agaagattat tccatccgaa aactaacctt tgccctggca
    86701 caagtcttct ttcgccgcgc gcccggggaa aagacgtgca aaaaggttgt gtaaaacagt
    86761 ctcgcaaacg tttgctttcc ctgttagaat tgcgccgaat tttatttttc taccgcaagt
    86821 aacgcgtggg gacccaagca gtgaccgata aaacctctct tagctacaaa gatgccggtg
    86881 ttgatattga cgcgggtaat gctctggttg gaagaatcaa aggcgtagtg aagaaaacgc
    86941 gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc gctgtgtgca ttgccgcaaa
    87001 aatatcgtga acccgtgctg gtttccggca ctgacggcgt aggtaccaag ctgcgtctgg
    87061 caatggactt aaaacgtcac gacaccattg gtattgatct ggtcgccatg tgcgttaatg
    87121 acctggtggt gcaaggtgcg gaaccgctgt ttttcctcga ctattacgca accggaaaac
    87181 tggatgttga taccgcttca gcggtgatca gcggcatcgc ggaaggttgt ctgcaatcgg
    87241 gctgttctct ggtgggtggc gaaacggcag aaatgccggg gatgtatcac ggtgaagatt
    87301 acgatgtcgc gggtttctgc gtgggcgtgg tagaaaaatc agaaatcatc gacggctcta
    87361 aagtcagcga cggcgatgtg ctgattgcac tcggttccag cggtccgcac tcgaacggct
    87421 attcgctggt gcgcaaaatt cttgaagtca gcggttgtga tccgcaaacc accgaacttg
    87481 atggtaagcc attagccgat catctgctgg caccgacccg catttacgtg aagtcagtgc
    87541 tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca cctgaccggc ggcggcttct
    87601 gggaaaacat tccgcgcgta ttgccagata atacccaggc agtgattgat gaatcttcct
    87661 ggcagtggcc ggaagtgttc aactggctgc aaacggcagg taacgttgag cgccatgaaa
    87721 tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc cctgcctgct ccggaagtgg
    87781 acaaagccct cgccctgcta aatgccaacg gtgaaaacgc gtggaaaatc ggtatcatca
    87841 aagcctctga ttccgaacaa cgcgtggtta tcgaataatg aatattgtgg tgcttatttc
    87901 cggcaacgga agtaatttac aggcaattat tgacgcctgt aaaaccaaca aaattaaagg
    87961 caccgtacgg gcagttttca gcaataaggc cgacgcgttc ggccttgaac gcgcccgcca
    88021 ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt gacagtcgtg aagcctatga
    88081 ccgggagttg attcatgaaa tcgacatgta cgcacccgat gtggtcgtgc tggctggttt
    88141 tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc gggcgtttgc tgaacattca
    88201 cccttctctg ctgccgaaat atcccggatt acacacccat cgtcaggcgc tggaaaatgg
    88261 cgatgaagag cacggtacat cggtgcattt cgtcaccgat gaactggacg gtggcccggt
    88321 tattttacag gcgaaagtcc cggtatttgc tggtgatacg gaagatgaca tcaccgcccg
    88381 cgtgcaaacc caggaacacg ctatttatcc actggtgatt agctggtttg ccgatggtcg
    88441 tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa cgtctgccgc cgcagggcta
    88501 cgctgccgac gagtaatgcc cccgtagtta aagcgccagc tctgccgctg gcgtttttca
    88561 attcacctgt aaatcgcaag ctccagcagt ttttttcccc cttttctggc atagttggac
    88621 atctgccaat attgctcgcc ataatatcca ggcagtgtcc cgtgaataaa acggagtaaa
    88681 agtggtaatg ggtcaggaaa agctatacat cgaaaaagag ctcagttggt tatcgttcaa
    88741 tgaacgcgtg cttcaggaag cggcggacaa atctaacccg ctgattgaaa ggatgcgttt
    88801 cctggggatc tattccaata accttgatga gttctataaa gtccgcttcg ctgaactgaa
    88861 gcgacgcatc attattagcg aagaacaagg ctccaacgct cattcccgcc atttactggg
    88921 caaaattcag tcccgggtgc tgaaagccga tcaggaattc gacggcctct acaacgagct
    88981 attgctggag atggcgcgca accagatctt cctgattaat gaacgccagc tctccgtcaa
    89041 tcaacaaaac tggctgcgtc attattttaa gcagtatctg cgtcagcaca ttacgccgat
    89101 tttaatcaat cctgacactg acttagtgca gttcctgaaa gatgattaca cctatctggc
    89161 ggtggaaatt atccgtagcg atagcatccg ttacgcgctg ctggagatcc catcagataa
    89221 agtgccgcgc tttgtgaatt taccgccaga agcgccgcgt cgacgcaagc cgatgattct
    89281 tctggataac attctgcgtt actgccttga tgatattttc aaaggcttct ttgattatga
    89341 cgcgctgaat gcctattcaa tgaagatgac ccgcgatgcc gaatacgatt tagtgcatga
    89401 gatggaagcc agcctgatgg agttgatgtc ttccagtctc aagcagcgtt taactgctga
    89461 gccggtgcgt tttgtttatc agcgcgatat gcccaatgcg ctggttgaag tgttacgcga
    89521 aaaactgact atttcccgct acgactccat cgtccccggc ggtcgttatc ataattttaa
    89581 agactttatt aatttcccca atgtcggcaa agccaatctg gtgaacaaac cactgccgcg
    89641 tttacgccat atttggtttg ataaagccca gttccgcaat ggttttgatg ccattcgcga
    89701 acgcgatgtg ttgctctatt atccttatca cacctttgag catgtgctgg aactgctgcg
    89761 tcaggcttcg ttcgacccga gcgtactggc gattaaaatt aacatttacc gcgtggcgaa
    89821 agattcacgc atcatcgact caatgatcca cgccgcacat aacggtaaga aagtcactgt
    89881 ggtggttgag ttacaggcgc gtttcgacga agaagccaac attcactggg cgaagcgcct
    89941 gaccgaagca ggcgtgcacg ttatcttctc tgcgccgggg ctgaaaattc acgccaaact
    90001 gttcctgatt tcacgtaaag aaaacggtga agtggtccgt tacgcacaca tcgggaccgg
    90061 gaactttaac gaaaaaaccg cgcgtcttta tactgactat tcgttgctga ccgcagatgc
    90121 gcgtatcacc aacgaagtac ggcgggtatt taactttatt gaaaacccat accgcccggt
    90181 gacatttgat tatttaatgg tgtcaccgca aaactctcgc cgtctgttat atgaaatggt
    90241 agaccgcgaa atcgccaacg cgcagcaagg gctgcccagt ggtatcaccc tgaagctaaa
    90301 taaccttgtc gataaaggcc tggttgatcg tctgtatgcg gcctccagct ccggcgtacc
    90361 ggttaatctg ctggttcgcg gaatgtgttc gctgatcccc aatctggaag gcattagcga
    90421 caacattcgt gccatcagta ttgttgaccg ttaccttgaa catgaccggg tttatatttt
    90481 tgaaaatggc ggcgataaaa aggtctacct ttcttccgcc gactggatga cgcgcaatat
    90541 tgattatcgt attgaagtgg cgacaccgct gctcgatccg cgcctgaagc agcgggtgct
    90601 ggacatcatc gacatattgt tcagcgatac ggtcaaagca cgttatatcg ataaagaact
    90661 cagtaatcgc tacgttcccc gcggcaatcg ccgcaaagta cgggcgcagt tggcgattta
    90721 cgactacatc aaatcactcg aacaacctga ataaccctat gccaatacac gataaatccc
    90781 ctcgtccgca ggagtttgct gcggtcgatc ttggttcaaa cagttttcac atggtcatag
    90841 cccgtgtggt agatggtgcc atgcagatta ttggccgcct gaaacagcgg gtgcatctgg
    90901 cggacggcct ggggccagat aatatgttga gtgaagaggc aatgacgcgc ggtttgaact
    90961 gtctgtcgct gtttgccgaa cggctacaag ggttttctcc tgccagcgtc tgtatagtcg
    91021 gcacccatac gctgcgtcag gcgctgaacg ccactgactt tctgaaacgc gcggaaaagg
    91081 tcattcccta cccgattgaa attatttccg gtaatgaaga agcccgtctg atttttatgg
    91141 gcgtggaaca tacccaaccg gaaaaaggtc gcaaactggt tattgatatt ggcggcggat
    91201 ctacggaact ggtgattggt gaaaatttcg aacctattct cgttgaaagc cgccggatgg
    91261 gttgtgtcag ctttgcccag ctttattttc ctggcggggt catcaataaa gagaattttc
    91321 agcgcgctcg catggcggca gcacaaaaac tggaaacttt aacctggcaa ttccgtattc
    91381 agggctggaa cgttgcaatg ggcgcttccg gtaccataaa agccgcccat gaagtgttaa
    91441 tggaaatggg cgagaaagac gggataatta ccccggaacg tctggaaaaa ctggtaaaag
    91501 aaattttacg tcaccgtaat ttcgcatcgc tgagtttacc gggtctttcc gaagagcgga
    91561 aaacagtctt cgttcctgga ctggcgattt tatgtggtgt gtttgatgct ttagccatcc
    91621 gtgaactgcg cctttctgac ggggcgttac gcgaaggcgt actgtatgaa atggaaggac
    91681 gtttccgtca tcaggatgtg cgtagtcgca ccgccagcag cctcgccaac cagtatcaca
    91741 tagacagcga acaggcccga cgagtgctgg ataccactat gcaaatgtac gaacagtggc
    91801 gggaacagca accgaagctg gcgcatccgc aactggaggc gctactgcga tgggccgcca
    91861 tgctgcatga ggtcgggttg aatatcaacc acagcggttt gcatcgccac tccgcttata
    91921 ttctgcaaaa cagtgacttg ccgggtttta atcaggaaca gcagctgatg atggcgacac
    91981 tggtgcgcta tcaccgtaaa gcgattaagc tcgacgatct gccgcgcttt accttgttta
    92041 agaagaaaca gttcctgcca ctgatacagc tattgcgcct tggcgtatta ctaaacaatc
    92101 aacgtcaggc aaccaccaca ccgccaacat tgacactgat tactgatgac agtcactgga
    92161 cactgcgttt cccgcatgac tggtttagtc agaatgcgct ggtactgctt gatctggaaa
    92221 aggagcaaga atactgggaa ggcgtggctg gctggcggtt gaaaattgaa gaagaaagta
    92281 ccccagaaat cgccgcttaa tgctttgcgg gccgacgaga aatgtcggcc cgcattattc
    92341 aggcactttc gcgaatgggt tcgatttcat tcagcgtatc aattaacggt tgcggcttac
    92401 caataagata accctgcata taatcgatcc ccaaagagag caccgcctcg cggatctctt
    92461 cgttttcaac gtactctgcc actacccgca ttttcttcat tcgcgccaga tggcaaatcg
    92521 acgccacaat ctgataatcc agactatttg acacaatatt gcggataaag ctgccgtcaa
    92581 ttttaagcag atcggcattc acatttttaa gccgtgcata gctggcgtag ccggtgccga
    92641 aatcatcaat cgcaatctgg cagcctaatt cctgaagatg ctgcaaggtg atttgcgcct
    92701 gcttaacatt ggtcagagca ttactttcgg tgacttcaaa aataagttgc cacgcttcaa
    92761 tctgatattt agccagcaac tgactgactt caacaggaaa acgagcctga cataccgagg
    92821 ttggagacag attaatagca aaacggtgag cgggcatctt cgctctgttt tcagccataa
    92881 attgcagcgt atgctcaatg acccacatgt cgatactcga cgataaacca aattcgtgcg
    92941 cgaccggcaa gaagctaccg gggccgatca gttcatcatt ctcacctttc atgcgcagaa
    93001 gaatttcatg gtaaacatca ccacgcatac cggtaatcgg ctgggccatc aggaaaaaat
    93061 ggttgtgttc cagcgcctgc tgtagtcgat tcatcatcgc gactttatct ttcaattcgc
    93121 gttgcaaata cattgccccg caacgctgca tattttccgg ggcgttggtc acgatggaaa
    93181 gttcggcgac cgtatttagc tctcccagca gcaggtagat
//
LOCUS       DAJTUP010000003        72981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTUP010000003 DAJTUP010000000
VERSION     DAJTUP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.003"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO468_000198"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO468_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6257453.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO468_000199"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO468_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR6257454.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO468_000200"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO468_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR6257455.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO468_000201"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO468_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR6257456.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO468_000202"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO468_000202"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR6257457.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO468_000203"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO468_000203"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR6257458.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO468_000204"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO468_000204"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR6257459.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO468_000205"
     CDS             complement(4598..5320)
                     /locus_tag="OO468_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6257460.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO468_000206"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO468_000206"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6257461.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO468_000207"
     CDS             6520..6738
                     /locus_tag="OO468_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR6257462.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO468_000208"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO468_000208"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6257463.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO468_000209"
     CDS             complement(7472..7672)
                     /locus_tag="OO468_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR6257464.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO468_000210"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO468_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO468_000211"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO468_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR6257465.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO468_000212"
     CDS             10101..12014
                     /locus_tag="OO468_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6257466.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSSEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO468_000213"
     CDS             12014..13036
                     /locus_tag="OO468_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6257467.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO468_000214"
     CDS             13030..13248
                     /locus_tag="OO468_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR6257468.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO468_000215"
     CDS             13302..14171
                     /locus_tag="OO468_000215"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR6257469.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO468_000216"
     CDS             complement(14226..14630)
                     /locus_tag="OO468_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR6257470.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO468_000217"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO468_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR6257471.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO468_000218"
     CDS             15615..17705
                     /locus_tag="OO468_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR6257472.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO468_000219"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO468_000219"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR6257473.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO468_000220"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO468_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR6257474.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO468_000221"
     CDS             complement(19673..20275)
                     /locus_tag="OO468_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR6257475.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO468_000222"
     CDS             complement(20265..20432)
                     /locus_tag="OO468_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR6257476.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21127)
                     /gene="ppiA"
                     /locus_tag="OO468_000223"
                     /gene_synonym="rotA"
     CDS             complement(20537..21127)
                     /gene="ppiA"
                     /locus_tag="OO468_000223"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR6257477.1"
                     /translation="MFKSTLAAMAAVFALSALSALSALSPAAMAAKGDPHVLLTTSAG
                     NIELELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPP
                     IKNEADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVK
                     GMDVADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21398..22579
                     /gene="tsgA"
                     /locus_tag="OO468_000224"
     CDS             21398..22579
                     /gene="tsgA"
                     /locus_tag="OO468_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR6257478.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22841..25384
                     /gene="nirB"
                     /locus_tag="OO468_000225"
     CDS             22841..25384
                     /gene="nirB"
                     /locus_tag="OO468_000225"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR6257479.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25381..25707
                     /gene="nirD"
                     /locus_tag="OO468_000226"
     CDS             25381..25707
                     /gene="nirD"
                     /locus_tag="OO468_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR6257480.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25833..26639
                     /gene="nirC"
                     /locus_tag="OO468_000227"
     CDS             25833..26639
                     /gene="nirC"
                     /locus_tag="OO468_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR6257481.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26658..28031
                     /gene="cobA"
                     /locus_tag="OO468_000228"
     CDS             26658..28031
                     /gene="cobA"
                     /locus_tag="OO468_000228"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6257482.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28278..28445
                     /locus_tag="OO468_000229"
     CDS             28278..28445
                     /locus_tag="OO468_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR6257483.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28740..30077
                     /gene="frlA"
                     /locus_tag="OO468_000230"
                     /pseudo
     CDS             28740..30077
                     /gene="frlA"
                     /locus_tag="OO468_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30098..31120
                     /gene="frlB"
                     /locus_tag="OO468_000231"
     CDS             30098..31120
                     /gene="frlB"
                     /locus_tag="OO468_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR6257484.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31170..32000
                     /gene="frlC"
                     /locus_tag="OO468_000232"
     CDS             31170..32000
                     /gene="frlC"
                     /locus_tag="OO468_000232"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR6257485.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31997..32782
                     /gene="frlD"
                     /locus_tag="OO468_000233"
     CDS             31997..32782
                     /gene="frlD"
                     /locus_tag="OO468_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR6257486.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32882..33613
                     /gene="phoB"
                     /locus_tag="OO468_000234"
     CDS             32882..33613
                     /gene="phoB"
                     /locus_tag="OO468_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR6257487.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33773..34858)
                     /locus_tag="OO468_000235"
     CDS             complement(33773..34858)
                     /locus_tag="OO468_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR6257488.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34870..36174)
                     /locus_tag="OO468_000236"
     CDS             complement(34870..36174)
                     /locus_tag="OO468_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR6257489.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36186..36539)
                     /locus_tag="OO468_000237"
     CDS             complement(36186..36539)
                     /locus_tag="OO468_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR6257490.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36550..37428)
                     /locus_tag="OO468_000238"
     CDS             complement(36550..37428)
                     /locus_tag="OO468_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR6257491.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37425..38651)
                     /locus_tag="OO468_000239"
     CDS             complement(37425..38651)
                     /locus_tag="OO468_000239"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6257492.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38652..39815)
                     /locus_tag="OO468_000240"
     CDS             complement(38652..39815)
                     /locus_tag="OO468_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR6257493.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39899..40261)
                     /locus_tag="OO468_000241"
     CDS             complement(39899..40261)
                     /locus_tag="OO468_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR6257494.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40278..41183)
                     /locus_tag="OO468_000242"
     CDS             complement(40278..41183)
                     /locus_tag="OO468_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257495.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41453..42457)
                     /gene="trpS"
                     /locus_tag="OO468_000243"
     CDS             complement(41453..42457)
                     /gene="trpS"
                     /locus_tag="OO468_000243"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR6257496.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42450..43208)
                     /gene="gph"
                     /locus_tag="OO468_000244"
     CDS             complement(42450..43208)
                     /gene="gph"
                     /locus_tag="OO468_000244"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR6257497.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43201..43878)
                     /gene="rpe"
                     /locus_tag="OO468_000245"
     CDS             complement(43201..43878)
                     /gene="rpe"
                     /locus_tag="OO468_000245"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR6257498.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43896..44732)
                     /gene="dam"
                     /locus_tag="OO468_000246"
     CDS             complement(43896..44732)
                     /gene="dam"
                     /locus_tag="OO468_000246"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6257499.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44839..46131)
                     /gene="damX"
                     /locus_tag="OO468_000247"
     CDS             complement(44839..46131)
                     /gene="damX"
                     /locus_tag="OO468_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR6257500.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46223..47311)
                     /gene="aroB"
                     /locus_tag="OO468_000248"
     CDS             complement(46223..47311)
                     /gene="aroB"
                     /locus_tag="OO468_000248"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR6257501.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47368..47889)
                     /gene="aroK"
                     /locus_tag="OO468_000249"
     CDS             complement(47368..47889)
                     /gene="aroK"
                     /locus_tag="OO468_000249"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR6257502.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48290..49528)
                     /gene="hofQ"
                     /locus_tag="OO468_000250"
     CDS             complement(48290..49528)
                     /gene="hofQ"
                     /locus_tag="OO468_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR6257503.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49440..49846)
                     /gene="hofP"
                     /locus_tag="OO468_000251"
                     /pseudo
     CDS             complement(49440..49846)
                     /gene="hofP"
                     /locus_tag="OO468_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49836..50276)
                     /gene="hofO"
                     /locus_tag="OO468_000252"
     CDS             complement(49836..50276)
                     /gene="hofO"
                     /locus_tag="OO468_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR6257504.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50260..50799)
                     /gene="hofN"
                     /locus_tag="OO468_000253"
     CDS             complement(50260..50799)
                     /gene="hofN"
                     /locus_tag="OO468_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR6257505.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50799..51578)
                     /gene="hofM"
                     /locus_tag="OO468_000254"
     CDS             complement(50799..51578)
                     /gene="hofM"
                     /locus_tag="OO468_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR6257506.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51698..54250
                     /gene="mrcA"
                     /locus_tag="OO468_000255"
     CDS             51698..54250
                     /gene="mrcA"
                     /locus_tag="OO468_000255"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR6257507.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54415..54975)
                     /gene="nudE"
                     /locus_tag="OO468_000256"
     CDS             complement(54415..54975)
                     /gene="nudE"
                     /locus_tag="OO468_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR6257508.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55295..57430
                     /gene="igaA"
                     /locus_tag="OO468_000257"
     CDS             55295..57430
                     /gene="igaA"
                     /locus_tag="OO468_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR6257509.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57495..58163
                     /gene="yrfG"
                     /locus_tag="OO468_000258"
     CDS             57495..58163
                     /gene="yrfG"
                     /locus_tag="OO468_000258"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR6257510.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58174..58575
                     /gene="hslR"
                     /locus_tag="OO468_000259"
     CDS             58174..58575
                     /gene="hslR"
                     /locus_tag="OO468_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR6257511.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58600..59469
                     /gene="hslO"
                     /locus_tag="OO468_000260"
     CDS             58600..59469
                     /gene="hslO"
                     /locus_tag="OO468_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR6257512.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59613..61364)
                     /locus_tag="OO468_000261"
     CDS             complement(59613..61364)
                     /locus_tag="OO468_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR6257513.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQGRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61743..63365
                     /gene="pckA"
                     /locus_tag="OO468_000262"
     CDS             61743..63365
                     /gene="pckA"
                     /locus_tag="OO468_000262"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR6257514.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63441..64793)
                     /gene="envZ"
                     /locus_tag="OO468_000263"
     CDS             complement(63441..64793)
                     /gene="envZ"
                     /locus_tag="OO468_000263"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR6257515.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64790..65509)
                     /gene="ompR"
                     /locus_tag="OO468_000264"
     CDS             complement(64790..65509)
                     /gene="ompR"
                     /locus_tag="OO468_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR6257516.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65737..66213
                     /gene="greB"
                     /locus_tag="OO468_000265"
     CDS             65737..66213
                     /gene="greB"
                     /locus_tag="OO468_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR6257517.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66310..68631
                     /locus_tag="OO468_000266"
     CDS             66310..68631
                     /locus_tag="OO468_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR6257518.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69069..69296
                     /gene="feoA"
                     /locus_tag="OO468_000267"
     CDS             69069..69296
                     /gene="feoA"
                     /locus_tag="OO468_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR6257519.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69313..71634
                     /gene="feoB"
                     /locus_tag="OO468_000268"
     CDS             69313..71634
                     /gene="feoB"
                     /locus_tag="OO468_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR6257520.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71634..71870
                     /gene="feoC"
                     /locus_tag="OO468_000269"
     CDS             71634..71870
                     /gene="feoC"
                     /locus_tag="OO468_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR6257521.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72073..72960
                     /gene="rpnA"
                     /locus_tag="OO468_000270"
     CDS             72073..72960
                     /gene="rpnA"
                     /locus_tag="OO468_000270"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR6257522.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17594 a        19777 c        18457 g        17153 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcagt gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt
    21121 tgaacatgag atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat
    21181 agccttcaag aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc
    21241 tgacgatcac tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga
    21301 ttgcaataaa acatttaaac agatcataaa atcacctaaa atcgcccgct cgcggcatat
    21361 cccgctatgc cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa
    21421 gctcacatgg attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg
    21481 gatggtgatg ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac
    21541 cttcaccttc ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga
    21601 aatcgtcccg ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc
    21661 cggtttgatg ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt
    21721 ggtcagcggc attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg
    21781 tcagcgcggt tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt
    21841 cccaatgatc gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg
    21901 catcgggctg gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcact
    21961 gggcaaacgt gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt
    22021 actgtttctc tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg
    22081 ggtgcctgag tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag
    22141 taacttctgg atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt
    22201 tgatttgcaa cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt
    22261 taacaccgga acaccagtac atatggcgtg gtcaattctc gctctgggct tcttctccag
    22321 cgcgatctat accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa
    22381 actggttaac tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac
    22441 cggcacgatt gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta
    22501 cgctgtcgtc tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa
    22561 caccctgacc tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc
    22621 tctcttcccc cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc
    22681 acaaacatga aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat
    22741 accccttaag gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat
    22801 cgttaaggta ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc
    22861 aattatcggt aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga
    22921 tgcggccaac tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt
    22981 acacctctca tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg
    23041 cttctacgag aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg
    23101 tcaggagaag gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat
    23161 ggcaaccggt tcctacccgt ggatcccacc aatcaaaggt tctgatactc aggactgctt
    23221 tgtctatcgc accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg
    23281 cggtgccgtt gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt
    23341 aggtattgaa acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca
    23401 gatgggtggc gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag
    23461 caaaaacacc cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc
    23521 cgacggcagc gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga
    23581 taagctggca acccagtgtg gtctggacgt tgctccgcgt ggggggattg tcattaatga
    23641 ttcctgccag acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa
    23701 ccgtgtattt ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat
    23761 tctgggtagc gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg
    23821 cgtagacgta ggtggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctatgt
    23881 ttacctcgac gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac
    23941 cctgctcggt gcggtactgg tgggcgatac cagcgactac ggcaacctgc tgcaactggt
    24001 gctgaacgct atcgagctgc cggaaaaccc ggattctctg attctgccgg cgcactcggg
    24061 tagcggtaag ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg
    24121 cttcgacgtc actaaaggtg atctgattgc tgccatcaac aaaggctgcc acacagttgc
    24181 ggcgctgaaa gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac
    24241 tcaggtactg aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga
    24301 acactttgct tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac
    24361 cttcgaagaa ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac
    24421 cgtcggttcg ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc
    24481 gctgcaggat tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt
    24541 gatcccgcgt tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat
    24601 cgcgcgtgaa tttaatctct acaccaaaat caccggctcc cagcgcctgg cgatgtttgg
    24661 cgcacagaaa gacgatctgc cggaaatctg gcgtcagctg attgaagctg gcttcgaaac
    24721 cggtcatgcc tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg
    24781 ccgctacggt gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg
    24841 catccgtacg ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga
    24901 agctcagggt aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg
    24961 cggtaacggc ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga
    25021 aacgctgatc aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact
    25081 gacgcgtacc gcgccgtggt tagaaaacct cgaaggcggc atcgattacc tgaaagcggt
    25141 gatcattgac gacaaactgg ggctgaacgc tcatctggaa gaagagatgg cgcgcctgcg
    25201 tgaagcggta gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt
    25261 caaacacttc atcaacagcg acaagcgtga cccgaacgtg cagatggtgc cagagcgcga
    25321 acagcaccgt ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc
    25381 atgagccagt ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc
    25441 gcactgttag gtgacaagca ggtagcgatt ttccgcccgt atcacagcga ccaggtgttt
    25501 gcgatcagca acattgatcc gttcttcgag tccagcgtgc tatcacgcgg gctgattgcg
    25561 gaacaccagg gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt
    25621 gatggcttgt gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa
    25681 gacggcgtgg tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt
    25741 tgcatggtcc tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta
    25801 ttttttgttt ttatttttta aaggataatc aaatgtttac agacactatt aataagtgtg
    25861 cggctaatgc tgcgcgcatt gcacgcctgt cggcaaataa cccgctcggc ttttgggtca
    25921 gctccgccat ggcgggcgca tatgtgggtc ttgggatcat cctgattttc acgctcggta
    25981 atttgctcga cccgtccgta cgtccgctgg tgatgggcgc gacctttggt atcgccttaa
    26041 cgctggtgat tatcgccggt tctgaactgt tcaccggaca caccatgttc ctcacctttg
    26101 gggtaaaagc gggcagcatc agccacgggc aaatgtgggc aatcctgccg caaacctggc
    26161 tgggtaacct ggtcggttcc gtcttcgttg ccatgctcta tagctggggc ggcggtagcc
    26221 tgctgccggt agataccagc atcgttcact ccgtcgcgct ggctaaaacc actgcaccgg
    26281 caatggtact gttcttcaaa ggtgcattgt gtaactggct ggtttgcctg gcaatctgga
    26341 tggcgctgcg cactgaaggg gcggcgaaat ttatcgctat ctggtggtgt ctgctggcat
    26401 ttatcgcgtc cggctacgag cactctatcg ctaacatgac gctgttcgcg ctctcctggt
    26461 tcggcaacca cagcgaagcc tacacgctgg cgggtattgg tcataacctg ctgtgggtga
    26521 cgctgggtaa tactttatca ggtgccgtat tcatgggatt gggttattgg tatgctacgc
    26581 cgaaagcgaa tcgtccggtt gcggacaaat ttaatcaaac tgaaacagct gccggttaat
    26641 tactaagggg tttttacgtg gatcatttgc ctatattttg ccaattacgc gatcgcgact
    26701 gtctgattgt cggcggtggt gatgtcgcgg aacgcaaagc aaggttgctg ttagacgcag
    26761 gcgctcgctt aacggtgaat gcattagcgt ttattccaca gttcaccgca tgggcagatg
    26821 caggcatgtt aaccctcgtc gaagggccat ttgatgaaag ccttctcgac acctgctggc
    26881 tggcgattgc agcgacggat gatgacacgc ttaaccagcg cgtcagcgaa gccgctgaag
    26941 ctcgtcgcat cttctgtaac gtagttgatg cgccgaaagc cgccagcttt attatgccgt
    27001 cgattattga ccgctcaccg ctaatggtag cggtctcctc tggcggcacc tctccggttc
    27061 tggcgcgcct gttgcgcgaa aaactcgaat cactgctgcc gttgcatctg ggccaggtgg
    27121 cgaaatatgc cgggcaatta cgcgggcgag tgaaacaaca gttcgccacg atgagtgagc
    27181 gtcgccgttt ctgggagaaa ttgttcgtta acgaccgcct ggcgcagtcg ctggcaaaca
    27241 acgatcagaa agccattact gaaacgaccg aacagttaat caacgaaccg ctcgaccatc
    27301 gcggtgaagt ggtgctggtt ggtgcaggtc cgggcgatgc cgggctgctg acgctgaaag
    27361 gactgcaaca aattcagcag gcagatgtgg tggtctacga ccgtctggtt tctgacgata
    27421 ttatgaatct ggtacgccgc gatgctgatc gcgttttcgt cggcaaacgc gcgggatacc
    27481 actgtgtacc gcaggaagag attaaccaga tcctgctgcg ggaagcgcaa aaaggcaaac
    27541 gcgtggtgcg gctgaaaggt ggcgatccgt ttatttttgg ccgtggtggc gaagagctgg
    27601 aaacactgtg caacgcgggt attccgttct cggtggttcc gggtattacc gcagcttctg
    27661 gttgctctgc ctattcgggt attccactca cgcatcgcga ttatgcccag agcgtacgct
    27721 taattaccgg acacttaaaa accggtggcg agctggactg ggaaaacctg gcggcagaaa
    27781 aacagacgct ggtgttctat atggggttga atcaggccgc gactattcag caaaagctga
    27841 ttgaacacgg tatgcctggc gaaatgccgg tggcaattgt cgaaaacgga acggcagtca
    27901 cgcagcgcgt gattgacggt acgctcacgc aactcggtga acttgctcag caaatgaaca
    27961 gtccatcgct aattattatt ggtcgggttg ttggcctgcg cgataaactg aactggttct
    28021 ccaatcatta atttaacccg ggccagagaa ttctggcctt cttaatgact ctttttatat
    28081 attcagcaaa taaaacataa ccccttaata aatattatct gctaatgggt tatattcacc
    28141 gttacagcga tttacaaaat taagtcattc gctcactgac gccaattaaa tattcctgta
    28201 tcgttcttaa cgagcagtct gcttatattc atataatcaa tgaatattaa ttaatatata
    28261 atacataggg aatgtaaatg aacaaattta ttaaagttgc actggtaggt gcagtactgg
    28321 ctacgttaac tgcatgtact ggtcatattg aaaaccgtga taagaactgc tcttacgact
    28381 acctgctgca cccggcaatt tctatttcta aaatcattgg cggttgcggt cctactgcac
    28441 agtaagtctc tgacagaaat cggctaacac cggtttaatt gcccgacgcc ctctggattt
    28501 tccgagggcg tatttgtttc tatgtccttc ttgcccccgc ccgtaaaaat attttcactt
    28561 taaattcaat tttataacta catccattag ccacagcgcg atcctgtgcg aaattttgtg
    28621 atcttcctcc acatcacata acataatatg ttgttatatt catcatgcat tgtcatgtta
    28681 ccttttaaat ggctgcaaac tctcccctac aacgtattgg acaagaaaaa ggtatcgcta
    28741 tgggaagcca ggaactccaa cgcaagctcg gattttgggc cgttcttgca atcgccgtcg
    28801 ggacaaccgt cggctccggt atttttgtat ctgtgggtga agtggcaaaa gcagcgggca
    28861 cgccgtggct tacggtgctc gcgtttgtca ttggcgggtt aattgtgatc ccgcaaatgt
    28921 gcgtctatgc ggaactatcc accgcttatc cggaaaatgg cgcagattat gtttatctga
    28981 aaaatgccgg aagccgaccg ctggctttcc tctccggctg ggccagcttc tgggccaacg
    29041 atgcgccgtc attgtcgatt atggcgctgg cgattgtcag caatcttggc tttttaacgc
    29101 ctatcgatcc gttgctcggt aaatttatcg ccgccggatt aattatcgcc tttatgttgc
    29161 tacacctgcg ctccgttgaa ggcggcgcag cgtttcagac gctaattacc atcgccaaaa
    29221 ttatcccgtt cactatcgtc attggccttg ggatcttctg gtttaaagcg gagaattttg
    29281 ccgcccttac caccactgcg attggcgcaa cgggcagctt tatggcgctg ctggcgggga
    29341 tctctgccac cagttggtcg tataccggca tggcctctat ctgttatatg accggcgaaa
    29401 ttaaaaaccc cggaaaaacc atgccacgag cgctgattgg ttcctgtctg ctggttctgg
    29461 tgctctacac cctgctggcg ctggtgattt ccggcctgat gcccttcgac aaactcgcca
    29521 attctgaaac gccgatttcc gacgccctga cctagatccc cgcactcggc agcaccgctg
    29581 ggctctttgt tgccatcacg gcgatgatcg tcattcttgg ttcgctttcc agctgcgtga
    29641 tgtaccagcc gcggctggaa tacgcgatgg cgaaagacaa cctgttcttt aaatgcttcg
    29701 gccatgcgca tccgaaatac aacacgccgg atgtctccat catcctgcaa ggggcgctgg
    29761 ggatcttctt catcttcgtt tccgatctca ccagcctgct gggttatttc accctggtga
    29821 tgtgtttcaa aaataccctc accttcggct ccatcatctg gtgtcgtaaa cgcgacgatt
    29881 acaaaccgct gtggcgtact ccggctttcg ggctgatgac caccctcgcc attgcgtcaa
    29941 gcctcattct ggtcgcctca acctttgtct gggcaccgat tcccggcctt atctgcgccg
    30001 ccatcgttat tgctactggt ctgcctgctt acgccttctg ggcgaagcgt agccgccagc
    30061 tcaacgcttt gtcgtaatgt tatctgaaga aaataaaatg ttggatattg ataaaagcac
    30121 cgtggacttt ctggtcaccg aaaatatggt tcaggaagtg gaaaaagttc tcagccatga
    30181 cgttccgctg gtgcacgcca tcgtggaaga gatggtgaag cgcgacattg atcgtattta
    30241 tttcgttgcc tgcggctcgc cactcaacgc ggcgcaaacg gcgaaacatc tggcggatcg
    30301 cttttccgat cttcaggtct acgccatttc cggctgggag ttctgcgata acaccccgta
    30361 tcgcctcgac gatcgttgcg cggtgattgg cgtttctgac tacggtaaaa cggaagaggt
    30421 aatcaaagcg ctggagctgg gccgggcctg cggagcactc actgcggcgt tcaccaaacg
    30481 cgcggacagc ccgattacct cggcggcgga atttagcatt gattatcagg ccgactgtat
    30541 ctgggaaatt cacctgctgc tctgctacag cgtggtgctg gagatgatca cccgcctcgc
    30601 gccgaacgcg gaaatcggca agatcaaaaa cgatctcaag cagttgccga atgcgctcgg
    30661 tcatctggta cgcacctggg aagaaaaagg ccgccagctt ggtgaactgg ccagccagtg
    30721 gccgatgatt tataccgttg ctgcgggtcc gctgcgtccg ctgggttaca aagaaggcat
    30781 tgtaacgctg atggaattta cctggacgca cggctgcgtg attgagagcg gagagttccg
    30841 ccatggcccg ctggagattg tcgaaccggg cgttccgttc ctgttcctgc tcggcaatga
    30901 tgaaagtcgc cacaccaccg aacgcgccat taactttgtt aaacagcgta ctgacaacgt
    30961 gatcgtcatc gattacgccg aaatttcgca agggctgcac ccgtggctgg caccgttcct
    31021 gatgttcgtg ccaatggagt ggctctgcta ctacctgtct atttacaaag atcacaaccc
    31081 ggatgaacgc cgctattacg gtggtctggt ggaatattaa tccctctccc ggcccggcaa
    31141 cgggccggat ttacgcaagg agttacccga tgaaaacagg tatgtttacc tgcggccacc
    31201 agcggctgcc tattgaacac gcatttcgtg atgcaagcga gctgggttac gacggcatcg
    31261 aaatttgggg cggtcgcccg cacgcgttcg cgccggactt aaaagcgggc ggcatcaaac
    31321 aaatcaaggc gctggcgcag acgtatcaga tgccgattat cggttacacg ccagaaacca
    31381 acggctatcc gtataacatg atgctgggcg atgaacatat gcgtcgtgaa agcctggaca
    31441 tgatcaagct ggcgatagat atggcaaaag agatgaacgc gggttatacg ctgatttctg
    31501 cggcccacgc gggctatctc acgccgccta acgttatctg ggggcggctg gcagagaact
    31561 taagcgaact gtgtgagtac gcggaaaaca tcggcatgga tctgatcctc gaaccgttaa
    31621 cgccgtatga atcgaacgtc gtgtgtaatg ctaatgatgt gcttcatgcg ctggcgctgg
    31681 tgccttcgcc gcgcctgttc agcatggtcg acatttgcgc gccgtatgtc caggcggaac
    31741 cggtgatgag ttatttcgac aaactgggcg ataaattacg tcatctgcat attgtcgaca
    31801 gcgacggggc cagcgacacg cattacattc ctggagaagg caaaatgccg ctgcgggaac
    31861 tgatgcgcga tattattgat cggggctatg agggttactc tacggtggag ctggtgacga
    31921 tgtatatgaa cgagcccaga ctctatgccc gccaggcgct ggaacgcttt cgcgcgctgc
    31981 tgccggagga tgagagatga aaaccctggc gacaatcggc gataactgcg tcgatatcta
    32041 cccgcaactg aataaagcgt tttctggcgg taatgcggtc aatgtggcgg tgtactgcac
    32101 tcgctacggc atacagccgg gatgcattac ctgggtgggt gacgatgact acggcacaaa
    32161 gctgaagcag gatctcgccc gcatgggcgt cgatatcagc catgtccata cgaaacacgg
    32221 cgttaccgca caaactcagg tggaactgca cgacaatgat cgcgtttttg gcgactacac
    32281 cgaaggcgtg atggccgact ttgccctgag tgaagaggat tacgcctggc tggcgcagta
    32341 tgacattgtg cacgcggcaa tctggggaca tgcggaagac gcattcccac agctgcacgc
    32401 tgcgggcaaa cttaccgctt tcgacttctc cgacaagtgg gacagcccgc tctggcagac
    32461 actggtgccg catctcgatt ttgcctttgc ctccgcaccg caagaagacg aagcgctgcg
    32521 tctgaagatg aaagcgattg ttgcccgtgg cgcaggaaca gtgattgtca cgctgggtga
    32581 aaacggcagc attgcctggg atggcgcgca gttctggcgt caggctcctg aaccggtgac
    32641 ggttatcgac accatgggtg ccggagattc gttcattgcc ggattccttt gcggctggtc
    32701 tgcggggatg acattaccgc aggcgatggc gcagggaacg gcgtgcgcgg cgaaaaccat
    32761 tcagtaccac ggtgcctggt aggtataacg ttggcgtgag catcttcacg ccaacgtgct
    32821 gttacttgcc ggaaaacgac cctataatcc gagtaattca ttctttattt cagggtcgat
    32881 tatgtcagct acggaccgct actctcatca actcctctac gctaccgtcc gccagcggct
    32941 gctggatgat atcgcgcagg gggtttacca ggccgggcaa cagatcccta ccgaaaacga
    33001 gctttgtaca caatataacg tcagccgcat taccattcgc aaagccatca gcgacttagt
    33061 ggcagacggc gtactgatcc gctggcaggg aaaaggcacc tttgtacaaa gccagaaagt
    33121 tgaaaacgcc ctgcttactg tcagtggttt taccgatttt ggcgtctcac aaggcaagtc
    33181 gacgaaagag aaagtgatcg aacaggaacg ggtcagcgcc gcgccgtttt gcgaaaagct
    33241 gaacatcccc ggaaacagcg aagtgttcca tctctgccgg gtgatgtatc tcgataaaga
    33301 gccgctgttt attgatagtt catggatccc gctgtcgcgt tatcctgact ttgatgagat
    33361 ttacgtcgaa ggaagctcca cctatcagtt atttcaggag cgttttgaca cgcgagtggt
    33421 cagcgacaaa aagaccatcg atatctttgc cgccacccgc ccgcaggcaa aatggctgaa
    33481 atgcgaactg ggcgaaccgt tgtttcgcat cagcaaaatc gcctttgatc agaatgacaa
    33541 accggtgcac gtctccgaac tcttctgccg cgccaatcgc atcaccttaa cgattgataa
    33601 taaaagacat taaccgtagg ccggataaga tgcgccagca tcgcatccgg cgatgctggc
    33661 gcactgaatt ttacatcccg cacgttcccc tcaccctaac cctctcccca aaggggcgag
    33721 gggaccgttc agtgcggaat ttagcttgat tgccgaacgg tgctatccag cattaaatac
    33781 tcgcaatact ctcacgcaaa atccgcagca ccgtctcttc accgctgcga ttcgggttaa
    33841 tgcgaatcgc acaatgttcg agttgtggat tcgcctggcg aaacgttcct gaaagtcgat
    33901 agaagagcgg cgggatttca tatttcgact ctgcaccgac cgggtaaggc aacgcgccac
    33961 gcttttgcgc ttcttccagc accctggcgg caatcagctg atgaaactcg acaatcaaca
    34021 ccttcgactg cgcattggca atgacggcgc ttttcacttc cgccaccgcg ccgccgttaa
    34081 gcaaagccag caaccgttcg gatactcccg cctgcaccgc atgcatcact ggcgcgaaca
    34141 ccagaccacg caagacttcc agcgcctgtg agccctggat ctggctaccg ccggagtaaa
    34201 gcgtggcgcg aatccgacta ataacatcgg catcgccaac cactgcacca acgccctccg
    34261 gcccaaacag cttgaagcag gagaatgtcg agacattcgc gccgcattca cagccgattc
    34321 gcgctacctt catcaccgca tagttatcat cggttaacgc tggaacacct gccgcgcgca
    34381 acgttgccag cacatctgcc agcacatagc tgtcctgcgg ctgctggcgc gtatgctgca
    34441 ccagcgcggc atcaggttgt tgctcgtcga cgacctgctt cagtgccgac aggtcattga
    34501 aatcagcagt aataagcgtc agccccatct gctcaataat aacccgtgtc gtcgggtaaa
    34561 caggcgcgtc atgcaccaga agacgctgcc ccggtttgag caaagccgcc agcccggcgc
    34621 gaatcgcgcc agtccccgcg ccctgcacca gcgccgcggc ctgtgcgtga aatgcatcag
    34681 ccagcacctg ctccacccgt tgggtaatac gcggttgatt cagacttgga gttaacccta
    34741 aatcaccgcc agcaagaaac tcgctgcctg gaaaatggcg gcaaatagtg tccaccagcg
    34801 caaactgctt ttgctgcgct tcagcaagag tcaggctttg caaaggaaac gtcttcatag
    34861 gtgccttcct taagccggaa cgaacaggcc aagccagtaa agaacattca gcagaatacc
    34921 ggtgatcatt actgccacga ccggtgccgc cattttctgt accggacggc ccagggattc
    34981 gttgaggaag taaatggcaa ccgcgataga gaatccggta taacccgcca tcttaattgc
    35041 tgcgaaaatc gaaccgacca gcagcgccac ttccatcagc atattcatgg cgttacggat
    35101 gttatccgac gcattacgca ccgacgggta gcgtcccagc catttgccga tcgaacgaag
    35161 cagcaagact tccgccgaaa taaccactgc gcctaatacc gctgcaacca tcggattcgg
    35221 cgagagatag cccaccgcat aaacaaaggt aaagcccgca actgcataca cacccgttgc
    35281 taaggcggtg gtggcaatca acggcacaaa ccccagtccg cgcataaatt ctgccagagc
    35341 cgcctgatta atcagcgttt gcgattgttc cggcgtcacg cctgcggaat acgctttctc
    35401 cagtgtgaag atcgaaactt cgctaccagc aaaaatcttc atactggcaa cggcggcaat
    35461 caatgctccc acgatggcga tataaggtaa gtttttgata atccgcgacg tgcgctcttc
    35521 aaacaccgaa agcccgctgg catcgatgtc attttcatca cgatgacgca ggtcgtgagt
    35581 tatcgcgatc cccagcagca tcaccatgcc aataaagatt tcgatggatt cagggttaag
    35641 atgtgggaaa tagcgcacga ctaccacacg ggtcatcaga acaaccacgg cggcgaccag
    35701 actttgcttc cagccaaact ggtagaaaat cgccaccagc gggaacagtg caaaagcaga
    35761 aaccaccggc gagcttaatt ccccgaggct acctaataca tccaccggca gcgcggtcag
    35821 cagctggtta actggcaaca ggcaagtaag gatcaacacg ccccagatag cgccaagacc
    35881 aaacgccatc aggctgttta tcgccagtac gccgaggata tcggtaggaa gaaagagtaa
    35941 ccatgcgttg agcagcccgg ttttcagggt aaaagagatc cccaccgagg ccacaaaacc
    36001 aatgctcaga ccaaaagcga tactccccgc ctcgcgacgg ttcatatagc cttcaatcag
    36061 ttgcggcagg atcgggtgga tgccgtcatg aaaaacagcc gccgagcgat gctccagaag
    36121 cgatgtcata cccgtcaggc acgccaccac gataatctga atatacagat ccatagtcgt
    36181 gccttttatt tcagatggtt aatcaacatc ggaatcgcgt gttcgacgtg ctcaacggaa
    36241 aggccaaacg ccactttccc ttcagcgatc attttggcga tatgctcgtc tttcgcttta
    36301 atgcctggtt tggcaatggt gcaacttttg ttatagccaa tcaccgcgat ggcaatcgac
    36361 aatgccgcgc ccgcaccggt attacacgcg ccaatgtaat aatctagttg cccggatttc
    36421 accttcattg ccgcttccat gtcgttgtga atgaaaactt caaaacagcc aggagccgtc
    36481 gcttcaatgg tttttttaat ctgctcacgc tgtaagcctg caacgccaat ctttttcatg
    36541 agtctgtcct tattggaaaa attgagaggg attttcacgt aacatcacat ccacatcggc
    36601 ctgactgaat cctgactggc gcaattgcgg aataaaggtg gtcaataaat agtcatagcc
    36661 ataaccacca ttggctttta aatgggagcg gcgcgtaata tccatcgaca gcatgacgcg
    36721 gttcagcaac ccacggtcac gtagcgcgtg gagcatcgca atgcgctttt cgtccgggta
    36781 gtaactgttc ttaccaatgg tgtcgaactg cacgtacgcg ccgagatcga tcatcttcaa
    36841 aatgttgtcg aggttgtctt tcagatcgca gtgaccaacg gtgacgcgcg aaagatcaac
    36901 tccgtgggct tgcaacagag ccagttgttc cagccccatg gtgctgaacg acgtatgcgt
    36961 ggagatcggg cgtccggtct ggttatgcgc cagcgcagcg gcaataaata ccttctcttc
    37021 cagcggcgta atctttcctt cgctggagcc gatctccgcg atgatcccgg ctttcagctc
    37081 cgtgccatcg ataccctgtt caatttcatc gaccatctcc tgcgccagtt cctgcacgct
    37141 gcgggtcacc acatgttccg ggaaaaacgc atcctggtaa taaccagtac aggccaccac
    37201 gttgatcccg gtttcgcgca ttacatcgag cataaattgc gcattgcgcc ccatgtaacg
    37261 gttggtcatc tcaatcacat tacgtacacc ccgggtcatc aggtcgttca tctcctggca
    37321 aatgaacgca tactgatcaa ggcggcagtc cacgttgttt ttaaagccgg agagatcaat
    37381 atgcagatgc tcatgggcca gggtgtaacc cgtcggatca aaactcatag ggtgtctcct
    37441 gcaaaccgga gggaggaaag aaaagagcga ccattttgtg gcggtggcgc acggaaaaat
    37501 tcacacacgg tagctcccac atcagaaagc gtggtgcgca caccgagctg cgtggcgacc
    37561 agcccttgct gataaaccag cactggcacc acttcacggg tatggtggct gtgaccaatg
    37621 gtcggatcgt taccgtgatc cgccatcacg accaggcaat catctggctg catcgcctca
    37681 acaagccggg caaggttacg gtcaacgacc tgcaaacgtt cggcataacg tgcgacgtct
    37741 tctgcatgac cagcgaggtc ggtttcctgg atattgatgc aaataaacgc cgtcggatgg
    37801 gtgttaaatt cgtcgagggt gatatccata atccgctggc tatccaccag gttttgccag
    37861 ctcacaccat aaggattgct gacgatatct gccaccttac ccaccagcac ggttggcacg
    37921 cccgcttcat acagtttttg cggcacctgc actttttcat caacaccgta gcccatatgc
    37981 acgacctgga agccgttgtc ataagcgcca gaacgcggcg cattaatacc aataaagcgc
    38041 ccttctttgc tttctgcggc atcgagaata cgctgactgt tggttaacag gccgccaaat
    38101 gtaatgaccc gaccgacctg tacctggtca cgcacgatac gaccaatttt gattgcgtcg
    38161 tcaaaagaga tcacagagag attggcggta atgttataga cttggcctaa atccgcctcg
    38221 agattatcgc caatcgcaac cgcctgattg accgacagaa attgcagatc atcgccacgg
    38281 cgctccacct gccagccagc ggaaactaat gcctgctcaa cacggtcaat cacatcgcga
    38341 aaaggcatcc gcagcggcgg taacgggcgc gtgcctaaaa tttcctgatg ccccataaag
    38401 gtatcgccac cttcatgttg cagctctgcc acgccccagg ttgcggaatc tgacggctgc
    38461 atatcgcctg gcgcataacc caatgtgttg attagcccca gcttctccag cgttggtagc
    38521 tgcaaatgcg gcaactggct caggatgtga ccacaggtat tcgccccggc atcttgcggg
    38581 cgcaccagcg tgacatcttt cattgcccct acgccaaagc tatcaatcac taacaccaca
    38641 aatcgcgcca tttatgcctc cagagggttg ccgaggctgt cataacgacc aactatttct
    38701 ggttcgccgc gctgaatacc ggacaccagc accacatcgc tacgggtgac aaaaatctgc
    38761 gtgcgaaaac agagcaccac tgcactgctc accggatact cgcctgccag cggcagggtg
    38821 tagtcgatac tgctgtcatc cacagttttg agattggttt cagtaatctt ttgattttct
    38881 ggcgtaaaca ccagcgcatg ttgcgcatga ccacgacggt agtaaccgcc gccgtagcag
    38941 tagctgtcgc cacggaaatg atgggagatt tcacttaacc agagcatcgc gatacgttca
    39001 ggctgatcgc cctgctggtt tgccggaata gtaccggtca gcgcatgacc gggttcggca
    39061 tgagtcgcac cgtattccgc cagcaatggc agcgaagtgc agctggtcgc tgaaggtacg
    39121 ttcagttgct caatcgcaat accagatttc gccagttgat cccgtgcttg taccagcgtg
    39181 tgaagattcg gtgttggcaa aactttccca gcagcctcat cccaaagcag gcaagggaaa
    39241 tgggttagtc cggctaaatg cagccccggc agtttcttaa tttcagcaat cacctcgtgc
    39301 agcgaatgct gtacaaaacc gctctcctgc cccggataaa gaaaatcatc gtcgctataa
    39361 actttgagca gcacaggctg gacccgtccg gccttaaccg ctgccgcaga aacttcccgc
    39421 gctttgtcga gagtaaacac ggtgatgatg tcggtgccct gttcaacggc gtcagcaacc
    39481 tgatgacaag ggatttgtac cagatgcccc tgatgcgcca ctggcaaacc agcgcggcgc
    39541 atgactcgcg cctctttgta atccaccgcc acaatgccgc tgtagcctaa tgccagcaat
    39601 ttttccgcca gccacggatt acgaccaaat tgcttggtca tcagatacag ttcaatgccg
    39661 taaagccgcg ccgtctcaat gagccgctta ccgttttcca gtatctgatc cacgtcgatc
    39721 acccagctgt ccggggcgat tttgccctgc tgccacaaac ttagtgcggc ggaaatcagc
    39781 gccgggttct ggcgtttcaa tgcttctaca aacatcttgt tgcctcgttt ctgttccttg
    39841 aattaaatat tcattttttt gaatatttag gttgtgcgac gtggatttgt cgggagcttc
    39901 aaacctcttc gttagcagcc atccataatc cataaagatt ggccagaaaa tagccttctt
    39961 cacacgggtt aacttccaga gcgaattcct tcaacaactc ctgatgcaat tgcacaacgg
    40021 cttgccagtg gctggattgc gccagttccg ccagcaactc gttatccagc ggctctattt
    40081 cttcaccacg gcgactgcgc atcagtgcac tcgccatatg tgtcatcgcc atcgttcctt
    40141 gctcactgcg caccggcaga tggcactctt tttccagtac gttgacgacc tgcatcatgc
    40201 ctttgcagac gtccttatca ataacgcctg cctcgcaaag caggttgagt ctggtttcca
    40261 tatcagatgc ctttcgttta ataactgggt tcctgttccc cactcaccac ccgttgttga
    40321 tgggcaagca gggcgtgttt atctacaaca gcacgcagca attgttgcat tgggtaatca
    40381 tcgactcgcg tcaggaccac ggcttcggtg gcctgtaaaa atcgcggatc gtctgttagc
    40441 ggcgtcgcct ctaatcccag catggtcagt tcgttttccg ccaccacgtt ccagatcacc
    40501 gcatcgacat cgcctttaac aatgcgttgt aaactctcgt gataagagag atcgactcgt
    40561 tccacatcac tatcgccaaa aaagacatcg gtcatgattt tctgatccgc cgaacgacta
    40621 tcgagaccta ctcgcttaac atttccagac tcacctttgc ggcatatcag ttgatgttca
    40681 ccaacatacg tatgcgggcc aagctcgagg gcgatgcaca aattgttttg gctgagataa
    40741 ctttccgccg ccaggcggga aactactgcc atgtcataga caccgttaag taaacactcc
    40801 acgcgaatat ccgcgccgcg catatgcgca tagtaaaaag gaatgccatc aaactgggct
    40861 ttcaatccgc tcgctaaacc ttcgtacaag cgggtatagg gcaaaggcat cgcacatacc
    40921 acgttgttga tatccacatg actcagcaat gctttgttat ccatctcgac aagataactg
    40981 ccattgcgcc cacggcgttc aatccgtatc gctccgcttg attccagcgt tttcaacgcg
    41041 gcctgcgtca ggccaaccga tgaacggcat tcagttgcca gctcatctat ggttttcagt
    41101 cgattaccgc atttttcacc caacaaataa cgggccagtg tcgtgatgac gacgccttct
    41161 tttttgataa acgttcgacg cataatatat tttcaataaa atgaatattt atatcttcat
    41221 taaaatgaag ataagtggcg acgacgagat gtggaaacgg cgaggcgctt cacatttttt
    41281 cgggttgttg cgaaaagagg ataaaaaaac cgggtttccc cggtttcaga gtgatgacga
    41341 agtgatttat tcgacaatgc gagcaaaggg taattcccac tggcgcttgc ctgctcccct
    41401 caccctaacc ctctccccaa aggggcgagg ggaccgtccg agcgttttga tattacggct
    41461 tcgccacaaa accaatcgct tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg
    41521 ctttttctgc gccgtctttc atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt
    41581 gatagcgttc ctgcaattca gtcagcatac cggaaacggc atcagccact tcacctttca
    41641 gatgaccata catcttgcct tcgaactgtt tttccagttc tgggatgctc tggcccgtta
    41701 ccgcagaaag gatatccagc aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa
    41761 ctaccggcgg ctcgtcggag tcagtgaccg cgcgtttgat tttcttcact accgatttcg
    41821 gatcttccag caggccgata acgttattgc ggttatcgtc agacttggac atcttcttgg
    41881 tcggctccag cagcgacatt acgcgcgcgc cagatttcgg aataaacggc tccggcacct
    41941 taaagatctc gccatacagc gcgttgaaac gctgggcgat atcgcgactc agttccaggt
    42001 gctgtttctg gtcttcaccc accggtacca gattagtttg atacagcagg atgtccgcag
    42061 ccatcagcac cggatagcca aacagaccag cgttgatgtt ctcggcataa cgcgcagatt
    42121 tatctttaaa ctgcgtcatg cgactcagtt cgccgaagta ggtatagcag ttcagtgccc
    42181 agcctaactg cgcatgttct ggtacgtggg actgaacaaa aatggtgctt ttctcaggat
    42241 cgataccaca agccagatac aaggccagcg tatccaacgt cgctttacgc agcttctgtg
    42301 catcctggcg cacggtgatc gcgtgttggt caacgataca gtaaatgcaa tggtagtcat
    42361 cctgcatgtt tacccactga cgcagcgcac ccatgtagtt accaatggtc aattcacctg
    42421 agggctgtgc gccactaaaa acgatgggct tagtcatttt tcgattcctg attttcgcta
    42481 tgcggaagcc ctaatgcggg cagaaggtca tttatagact gataaattac atcaggctgg
    42541 ctgagatcga tagcctcgcc gtagttgtat ccgtaggtta agccaactga tgggcaacct
    42601 gccgcttttg ccgcctgaat atcattccgt gagtcgccga caaacagcat ctgttgtggg
    42661 gcaattccca tccgctcagc caccagtaac agcgggtccg gatgcggttt tttgttttgc
    42721 acatcatcgc cgccgataac cacgctaaag tatttggcaa tatctaaggc ttcgagcagc
    42781 ggcgcgacga acggcgtcgg tttgttggtg accaagccta gcggcaggcc tttagcctgc
    42841 aacgcgccca acgtatcggc aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg
    42901 ccatagtagc gatcgaacag tttacgcaga atacgtacct gttcttctgc cggaatgtcg
    42961 tcatcaacgg gcggtttccc cattgtttta cgcagagtcg cacgttcctg acgcgcccag
    43021 gtcaatgcgc gctccatcag aacatctgcg ccgttaccaa tccaggtaat aacgcgttct
    43081 tcacctgcga cgggcaactc cagcgcatac agcgccatat ctaccgcagc agcaagacca
    43141 ggagcactgt cgaccagcgt accatcaaga tcaaaagcga cgccgcgaat atcttcaaac
    43201 ttattcatga cttacctttg ccagttcact gcgcatttca tcaatgactt ttttatagtc
    43261 tggctggtcg aagattgccg aaccggcgac gaacatatcc gcgcccgccg cagcgatttc
    43321 gccaatgttg ttcaccttca cgccaccgtc cacttccaga cgaatgtcaa agccagactc
    43381 gtcgatacgg cgacgtactt cgcgcagttt atccagtgtt tgaggaatga aagactgacc
    43441 gccgaaacca gggttgacgg acatcagcag gatcacatcc agcttatcca tcacgtaatc
    43501 cagatagctc agaggtgtcg ccgggttaaa taccagaccc gctttacagc cattttcttt
    43561 aatcagttgc agcgtgcggt caacatgctc ggaggcttct ggatgaaagg taatgatgct
    43621 ggcaccagcg gcagcgaaat caggcacaat gcgatcgacg ggtttcacca tcagatgtac
    43681 gtcgataggg gcggtaatgc cgtagttacg caaggatttc agcaccattg gcccaatcgt
    43741 cagattggga acatagtggt tatccatgac gtcaaaatgc acgacatcag cgccagctgc
    43801 cagggctttt gcggtatctt cacccaggcg ggcaaaatca gccgacagaa ttgagggggc
    43861 aatcaaatac tgtttcatcc gcttctcctt gagaattatt ttttcgcggg tgaaacgact
    43921 cctggtttgt acaaagccag cagttcgtcc acctttttac gtgtgccgcc gttgctgctt
    43981 atactgcgtc gaactttgac gacatgcaat tttgcgcgct gataccactc acgcgttaac
    44041 atcgtatcgt gattggagat cagcactgga atatggcgct caaccagacc ttcggcgatc
    44101 tccgccagat gcgcttgttg ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg
    44161 gcggtcgcag acagcggtgc ataaggcgga tcgcaataga cgacggatgc atcatctgcg
    44221 cgctccatgc tatcggcgta agactcacaa tagaaaaagg cattctgcgc tttttcagcg
    44281 aagtgataca actctgcttc cgggaaatag ggttttttgt agcggccaaa cggcacgtta
    44341 aactcaccgc gcagattgta acgacacagg ccgttgtaac cgtagcggtt caaatataaa
    44401 aacagtaccg cccgacggaa cggatcctgg cttttgttga actcttcgcg gaactgatag
    44461 taaacctcgg cgcaatttgt ttcgggaaca aacagctcgc gtgcggcctg tacgtactca
    44521 tcagtacgca tcttcacaat gttatagaga ctgatcaggt cgctattgat atcggcaagg
    44581 atataacgag aaaagtcggt gttgagaaac accgacccgg cacctacaaa aggctcaacc
    44641 agacattcgc ccttgggcaa atgccgttta atatcatcaa gcaggggata cttgccccct
    44701 gcccacttca aaaaagcgcg atttttcttc atgccgatta actaattaca ccttctccgg
    44761 ctgtggagaa agctccgaca gcatccagcg cttcaggcag ctcccgcaac gataaaccat
    44821 tgcgggagat aaccttgatt acttcagatc ggcctgtacc tgacgcagcg gtttcgccca
    44881 cgggtttttg gcctgaacat ctgctggcaa tgtagatacc gcttttttcg cctcttcttt
    44941 cgaagcgtac acgccagaaa ccaggacata ccacggctga ccattacgcg tcgtttcata
    45001 gacaacgtag tttttcagat tctctttctt cgcccaaccg ttcaggttgt cgtagttaga
    45061 ggaactgctc agctgcagag tgtaatggct ggacggtgcc gatttcaacg aaccaacatt
    45121 acctgcggtc ttccctccag cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg
    45181 tactggagcc gtagtcgccg tctcttttgg tgctggcgta gaagtcgcag ccggtgcctt
    45241 cgtgctcgcc acaggggcag ctggttcagg ttcagtacgc ttcggcgtct gtgctaccgg
    45301 cttcggctcc gttttcacgg ttgcttgcgg ttttttcggt tcaatcaccg cctgctgacg
    45361 aactgggcgc gtagtggacg gacgttcagc ggtttgcgtt ttcgccgtgt cacgcgatgc
    45421 attgccattg cgaacaggcg caaccgttgc gggttcagtc ggcaacgtgg aattgaccgc
    45481 cacattgttc agctgttgct gattttgtgg ctgggtcagc gcattgttca ggtcaccctg
    45541 cacttcaaca cgttgttgac catccgttgc caccggggtt tgcccttgag tcggcgtaga
    45601 agagatcggt ggcagagaaa catcctgctg agtattttcc gcagacgtgg ttcccggcgc
    45661 tggctgcacg ccattcgcct gatcggtcgc attgccagca agatcaatac tcttctcgcc
    45721 agacgcggtt tgatcgctgg aagtggtcga gggggctttt agcgcagaac cgataccgat
    45781 gatcaacagc agtagaacca gaatgccgac gcccatcatc atatactgac gagaagcggg
    45841 tttactggct gcttttttgc gcttacgcgg acgacgctct acgcgctctt catccacggt
    45901 ttcatcttcg gattcgtcaa tttcttcttc gatttccggt tcctcattgc gctctttttg
    45961 cgcacgagtc ggacggcgat cgtcagtgtc atcaagttca atatcatcaa aattgatctg
    46021 cggttcgcca cgttcagtac gctcagaacg ttcagaagat tgacgagaac gaccagtacg
    46081 acgatcgctg ggatcgggtt tcagctcgtc ttctggtttg aattcatcca tttaacaccc
    46141 cactaaaagg ttaatgctta ccacgttgca attaacctga agctaataga ccgcttgata
    46201 agcggcctga cctttcttgt tgttacgctg attgacaatc ggcaatggcg ttaagaacaa
    46261 gctcgtgcga aacgccgctg cgaacttcac tcttaccaat tgccaacgga agaattaagc
    46321 gcatctctcc cgcaaggact ttcttgtcac gcagcatatg cggtaaatac gcctgcgcgg
    46381 acatttcgcg cggcccattg accggtaacc cagcccgcgt gagcagggtt ataatacgct
    46441 gcgtttcggc agaactaaac tgcccgagac gttccgacgt ccgcgccgcc atcaccatac
    46501 ccgcagcgac cgcttcacca tgtaaccaat tgccataccc catttcagct tcaatggcat
    46561 gaccaaaggt gtgtcccaga ttcagtaaag cacgtaaccc ggtttcgcgc tcgtcggcgg
    46621 cgacaacttc tgccttcagt tcacaacaac ggcgaataca gtacgccatt gccggaccgt
    46681 ccagacgcaa caacgcatcc agattctctt ccagccagtt aaaaaacgca ccgtcaagaa
    46741 taatgccgta tttgatgact tctgccagcc ccgacgctaa ctcacgcggg ggaagcgttt
    46801 tcagacagtc gagatccacc accactgaag caggctggta gaacgcgcca atcatgtttt
    46861 taccgagggg atggttgacc gcagttttgc cgccaacgga ggaatcgacc tgcgacagta
    46921 acgtcgtcgg gacttgaatg aaacgaacac cgcgctgata actcgccgcc gcgaagccgg
    46981 tcagatcgcc cactacgccg ccgccaagcg ccaccagcgt agtatcgcga ccatgcggct
    47041 tttgtaacaa cgccgtaaag acggtatcga gtacagctag gcttttatac tgctcgccgt
    47101 cagggaggat aacgctatcg acgttaacac ccgcctgttc aagtacgccg cggaccttat
    47161 cgagatacag aggagccagg gtttcgctgg tgaccaacat gacctgctcg cccgatttca
    47221 gcggtaagaa tgaagctggt tcattaaaca aaccagatgc gatggtaatt gggtaactac
    47281 gttccccgag agtaacgaca atcctctcca taacgcgaca tccaccttaa ttactgtacc
    47341 cgcagacgag tgtatataaa gccagaatta gttgctttcc agcatgtgaa taatctggtt
    47401 tgcaaccact ttagcgcttt gatcatcagt acgaatggtc acgtcggcaa tctcttcata
    47461 cagcggattg cgttcattgg ccaacgcttc cagaacttca cgcggcggtg tttcaacgtg
    47521 cagcaacggg cgttttttat cacgctgcgt gcgtgcaagt tgcttttcga tggtcgtttc
    47581 aagataaacg acaacgccac gagcggaaag acggttacgc gtttcacggg atttcacaga
    47641 gccgccgcca gtagccagca caataccctg tttctcggtc aactcattga tgaccttttc
    47701 ttcgcgatcg cggaagcctt cttcgccttc taaatcgaaa acccagccca catcagctcc
    47761 ggttcgtttc tcaatctctt gatcggaatc gtaaaattcc atattgagtt gttgagctaa
    47821 ctggcgccca atagtgcttt ttccggcacc cataggccca accagaaaga tattgcgttt
    47881 ctctgccatt ttttcggtac tactaagact attcgttaat gataaacccg cttcgctcag
    47941 agagcgccgc aggacatgaa ctgaaacctc ataagatatt gcgagagtca gactgaaaat
    48001 tatctcaata ctccagcggg tttggcaact gaataaatca cccatccctc tgcatatctg
    48061 gtcgctgcaa gcgcgctgcc ttgctaccac cgctctggcg ataaatcacc gggtaagatt
    48121 agcgtaaaaa agacagcaaa atgccgcctg aatgataaat catcatcatg gaacgcaggc
    48181 agcgaagagc accaactctc aaatcggtac tccttgtatg ctaaatacct gcgcgcgtca
    48241 aatagatgaa acacgttcag cataaaaaca ttaccgcttt tacggctgtt tactcactgg
    48301 aaaccagtcg tggcgtgata aacaccacta actcgcgtcg ttcatcttct tttccgtcat
    48361 gacgaaataa ttgcccgaac cagggaatgt cgccaagcaa cggtacgcta tcctgacccg
    48421 atttattttt acgggtaaaa atgccgccca gcgccaacgt ttctccgctt tttacctcga
    48481 cctgcgtttc gatctcctgc ttatcaatcg ccagcacttc gccatcggcc tgctgtagca
    48541 cctgccccgg aacgttctgg ctgatgtgta atttcagccg gatgcgacct ttttgtaaca
    48601 ccgtgggcgt gacctccatc cccaggacgg cctctttaaa ttccaccgac gtcgcgccac
    48661 tttccccgct ggaaacctga tatggaattt cgctctcctg tttaatgctg gcaggctgaa
    48721 gatgtgaggc cagcagacgc ggactggcga taatatccag ctgctgtttt tgttcgagcg
    48781 cggaaagctc aagatccagc aagcgtccgt tgatgcgccc aatgttaaaa ccgacatgcg
    48841 ttgtcgccgt cgctacggag aggtcgctac caagcgtggt gacttgccca acgccaccag
    48901 cgtgttgcgc atcggccagc gtccatttca cgcctaactc acgcaaactt ttttcattaa
    48961 tggtgacaat atgcgccgac agctcaacct gcccgaccgg cagatccatt tgcgctaccc
    49021 actgttcaag cgcgcttaac gccgttttgt tatctcgtag caaaaggcga ttggtgcgtt
    49081 tatcgacggt catactccct ttggcactca gtagcttctc ccccgctttc gccagttctc
    49141 ccgcgtcggc gtattgcagg gttatactgc gattttccag cggcagattt gcctgcgccc
    49201 gcgcctgctc cgcctcctgg cgggcgatat tgtcattctg ccaggcaatg gaatgcactg
    49261 agagaatgtt gccttcctgc cgcgttatca gtccggcgct tttcactaca gtttgtagtg
    49321 cctgcttcca gggaacatct gttagatgta acgacaccgt accgctgacg tctggcgaca
    49381 cgaccaggtt caacttctcc tgttcagcca gcgcctgcaa cacctgagct accggaacgt
    49441 catccaccat cagcgtcact ttttgcggct ttgccgcctg tacgccgggt atcagcatca
    49501 acagtagtgc ggctatccat tgcttcattt gtatctcctt gccgttgcca caaccattgt
    49561 ggcggttcgc agtttgtccc ggtacccagc gttagtacgt ctggcgtcag ctgtaaaatt
    49621 gtccagccgt tttccagcac atcgttttgc tgcacccgtc gccatttctt ttgcccgtct
    49681 tttattacac cgatgatgcg ctcgcctcgc cctaccatcc cctgatagcg ccactggcta
    49741 agttcgctaa tccggcatat agatcttccg gcgatttaaa agggtcacgc ataccggtta
    49801 aaaggcacaa tgcaatacct gccaacaacc agcgtttaac cctcatgcgg catctccagt
    49861 tgtagcgtga acaaaagatc atcaccttcc acgcttaacg aaaaacggct cacgctgacg
    49921 ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc
    49981 aactcacctc cctgcgcgga tggatgccag gaaaccagtt gcgcgccgga taactgaaaa
    50041 tccagtggcg aaaagggcag cgttttttcc tcgctgaagg gagtggtgtc taccaggcga
    50101 tacagtgcgg cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct
    50161 tcatggtgtg tcgatgatag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag
    50221 caaaactggc ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct
    50281 aaccttcctt gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc
    50341 tccccgctga ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa
    50401 cgcggtaatg cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt
    50461 tgtcagccag gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc
    50521 gcgggtaaat tgtcgctggc gctgccgttg cgaacgctgt tcgcgtaact gctgttgctc
    50581 cagcaaacgt ggcttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa
    50641 aactgcgtct atgcgcgctt cggcgctgcc tgtcagacgc agtattagcg ttatcccgac
    50701 ggccagcagc agaggcgcaa cgaacatcag caaccagaaa cgcagaaaag cggtccggcg
    50761 ttgctgtcgc cagggcaaaa aattaattgg cggattcatc agtacgcctt cccaagcgcc
    50821 agccccagcg cgatggcaaa gtctccacca ggcggcggta gcggcggctg acgaacagaa
    50881 acggcctccc aggggtcaaa tccgccttcg ccacatatcg cgacgctgtc gggatcaacg
    50941 gataacgctg ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag
    51001 ctatagcgcg tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga
    51061 gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcactaaca
    51121 tgaacacgca acctttctgc cagcgtaagc agcgttgcca gctctttgct ttgcgcggca
    51181 gtcacgttat aagcggggct gagtgagtct tcgctgtaat cgaagcgcag ggagtccgga
    51241 tccatatcca gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcacca
    51301 agggacatcg acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac
    51361 ataatgtgat gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagctgc
    51421 tgcgcatcaa caatccgccc atctttgata atgtcgtgcg ccagcggcaa ccgccaccag
    51481 cgttgcaaaa agcattcttt tgcgccccgc acgatcgcaa ccgctgccgc ttcttgctgt
    51541 tgtaaatgca aaccaatttg ccagatctta aatgtcattg tgatgatctc cttatcaccc
    51601 gtcactctga cgggtatatc aatgcgtctg gcttgccttt atactaccgc gcgtttgttt
    51661 ataaactgcc caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt
    51721 gatccttgca gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta
    51781 catcgagcca caactgccgg atgtggtgac attaaaagat gttcgcctgc aaattccgat
    51841 gcagatttac agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc
    51901 ggttacgttg gatcaaatcc caccggagat ggtgaaagcc tttatcgcga cggaagacag
    51961 ccgcttctac gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc
    52021 gctgttctcc ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa
    52081 tttcttcctc agtccagaac gcacgatgat gcgtaagatt aaggaagtct tcctcgcgat
    52141 tcgcattgaa cagttgctga cgaaagacga aatcctcgag ctttatctga acaagattta
    52201 ccttggttac cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt
    52261 cgaccaactg acgctgaacg aaatggcggt gatagccggg ttgccgaaag cgccttccac
    52321 ctttaatccg ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg
    52381 gatgctggat gaagggtata tcacccagca acagttcgat cagacacgca ctgaggcgat
    52441 taacgctaat tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt
    52501 gcgccaggag atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta
    52561 caccaccatc acccgcaaag tgcagcaggc cgcgcagcag gcggtacgta ataacgtgct
    52621 ggactacgac atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga
    52681 gtcggcgtgg gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc
    52741 gctgctgcct gccgcagtca ccagcgccaa tcctcaggga gcgacggcga tgctggcgga
    52801 cgggtcgacc gtcgcattga gtatggaagg cgttcgctgg gcgcgtcctt accgttcgga
    52861 tactcagcag ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat
    52921 ctgggttcgt caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc
    52981 gctggtgtcg atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt
    53041 caatcagagc aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa
    53101 accgttcctc tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga
    53161 tgtgccaatt tctcgctggg atgcaggtgc cggttctgac tggcagccga agaactcacc
    53221 accgcagtat gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt
    53281 gatggtacgt gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt
    53341 cggcttcccg gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt
    53401 caccccaatg caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctagtgga
    53461 cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc
    53521 gaaagtagcc tgcccggaat gcgatattcc agtgatttac ggtgatacgc agaaatcgaa
    53581 cgtgctggaa aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc
    53641 tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca
    53701 ggagtacgca ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa
    53761 caccaatatc tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt
    53821 gcagcgtcgc gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt
    53881 ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg
    53941 taatctcggt catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg
    54001 cggtgccaag agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt
    54061 gccggagcag ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac
    54121 cgggcagtta gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc
    54181 gacacaacag gcggtgcacg aggtgggaac gaccattatc gataatggtg aggcacagga
    54241 attgttctga ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt
    54301 ttatgccgga tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat
    54361 tgcaggaacc ccataagcct gataagcgtt gtgcatcagg caaacttcac gcatttacac
    54421 tcgcccctgc cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc
    54481 gttgaagtca gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg
    54541 tagcggctct ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat
    54601 attcattttg ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc
    54661 gttcgcacca aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc
    54721 acccggatca attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta
    54781 ttcgcggatc aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt
    54841 ggttggacgc attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct
    54901 ctcgacggta aacagtcggg aacgggctac agtttcaaca ttcagaatgg tgggtttttg
    54961 taatgatttg ttcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg
    55021 acacgctttc ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatc
    55081 gtcctcaaat caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatattc
    55141 cgccttcaga gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt
    55201 taagctccac gcttgccgat agccaaccgc agaatcatgt attgtgtccg gtgcgactga
    55261 ccacgcctga cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc
    55321 tgctttgctg gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg
    55381 tcagctgccc tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga
    55441 acgtagcgcc gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc
    55501 aacggaagct agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat
    55561 gatgctgaca cacgctatca cacgttacgg catctctacc gacgatccga ataaatggcg
    55621 ttactacctc gattcggtag aagtccacct gccccccttc tgggaacagt acatcaacga
    55681 tgagaatacc gttgaactga ttcataccga ttcgctgccg ttggttattt cactcaacgg
    55741 tcatacgctg caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc
    55801 cacgcaggcg tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa
    55861 agaaacgcat gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat
    55921 cgtcgcctcc ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg
    55981 gctggcaggc ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc
    56041 ggcaaaatcc tccctgcgag aaattcattg tctgcgcggt acaccccgtc gttggggatt
    56101 gtttggcgaa aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt
    56161 ctatcccgca cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga
    56221 tatctatctc gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt
    56281 aaaaaacttt ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct
    56341 agtgctgttt atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc
    56401 atggatgaaa ggcgcacaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg
    56461 cgtgcgggta ggcgatacat tgcgtattag cggtacggga atgtgtaata ttcgcacttc
    56521 cggaacctgg agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat
    56581 ctggaatgac gcccgctcat taccattacc agaatctgaa ctggtcaaca aagcgacggc
    56641 attgactgaa gcagttaatc gccagctgca ccctaaaccg gaagatgaat ctcgcgtcag
    56701 tgcctcatta cgttcagcga ttcaaaaatc cggcatggta ttgcttgatg attttggcga
    56761 cattgtactg aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa
    56821 tgcgctggtc aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc
    56881 cgggaagctc gatggcgtga atgtgttatt acgcccggtg agtgctgaat cgctggataa
    56941 cctggtggca acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc
    57001 actaaacagt ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt
    57061 tgatcagccc tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc
    57121 tttccagaaa ctggcacaaa tgctgatgca tacgccgttt aacgccgaag gtatcgtcac
    57181 aaaaatcttc actgacgcca atggtacgca gcatattggc cttcatccga tcccggatcg
    57241 ttccggcctg tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc
    57301 catttacaat ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgga
    57361 gattcaggcc tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct
    57421 tatcgaataa cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc
    57481 tggagatttc cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg
    57541 atatggacgg cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc
    57601 ctgaaacatg gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc
    57661 agcaatatca cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc
    57721 aactgggtct ggatatctgt gcgatgacca ccgagatggg accgcgcgcc gtactgcgtg
    57781 aagataccat tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca
    57841 ccaatgctca tccgcacaac ctggcggtaa aacttgagca tacgggtctg gacgcacacc
    57901 ttgatttatt actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc
    57961 atgcggtggc cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg
    58021 aagcgattct cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc
    58081 ctgattccgg gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc
    58141 gcctgatccc ctcgctaatg tgaaggagac gccatgaaag agaaacctgc tgttgaggtt
    58201 cgactggata aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa
    58261 atgattgaag gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc
    58321 gagctgaatg ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag
    58381 gcgattactg aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg
    58441 gaaagtgtag agaaacgcga aaaaatggcg atggcacgta aacttaatgc cttaaccatg
    58501 ccgcacccgg accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac
    58561 ggcgacagtg aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta
    58621 catcgctatc tgtttgaaaa ttttgccgtt cgcggcgaac tggtaaccgt ttcggaaacc
    58681 ctgcaacaga tccttgagaa ccacgattat ccgcagcccg ttaaaaacgt gctggcagaa
    58741 ctgctggttg cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta
    58801 cagctgcagg gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa
    58861 atgcgcggtg tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg
    58921 gtcggcaatg gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc
    58981 gtagttggtc tggaaggtga taccctggcg gcctgcctag aagattactt tatgcgttct
    59041 gaacagctgc cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca
    59101 ggcggtatgt tgttgcaggt aatgcctgcg caaaataccc aacaggacga ctttgaccac
    59161 ctggcgacgc taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa
    59221 gtgttgtggc gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag
    59281 ttcaaatgca cctgctcgcg tgaacgttgc gccgatgcgc tgaaaacgct gcctgatgaa
    59341 gaagttgata gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt
    59401 aaccactatc tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca
    59461 gatccgtaag ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgact
    59521 taatggcctg atgtgctgcg cttaccagcg cctatgaaat aaatcgcaaa ttattttggt
    59581 cagatacagc gtcgtatccg ataaaagcgt tgtcacttac ttaagtttat ttccagcgtt
    59641 tcaccatcta ccgtcagatc gcgccacgct ttcggcctcg tccccagttt tccatccttc
    59701 gcagctgtga gtaatttttc tttcgttatt tcgttcggaa gtaaactcat atcaagcgcg
    59761 tcccattctt ttctgtctga gtcaaagcca tagacaatga ctctgtcatc attaaaagca
    59821 aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag tacgcaggcg
    59881 tctttttcaa tacaggtcat cacgttatag cgaccctgga ttaaggccga ccagaatgtc
    59941 gcatccggtt taacagaacc aggggcaatt aacacatttt cggcgaatac tttttccgat
    60001 acctgttcct gaagatgttg ctctccatct aacgccatca gcagatcccg cgcgcgtttc
    60061 gggtctttca taaacccggc atcgcttttc agcgactcaa gcgccgcacg tccatagcga
    60121 ccgctctgtt cgagcatgta gatagttacc tggtctggcg tgtttttgcc gctctgataa
    60181 cgcgccatat gactgttcac gctaatacgc atactgtcca gcaccggcga attaagaagc
    60241 accagtatca ccaacaccaa taacgaaacc gcaaggttca ctttgccctg aagaacaagg
    60301 ggattttgcc cattacgcca gacgatactg acaaaatacc ccagcgacca caccagtaac
    60361 accagctccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg agcgacccgc
    60421 agccataacc cccaggtggc gacgaacaca tacagcggag caaccagcaa agcggtttta
    60481 atcaggcaac gcaacggccc cgtccaggga agtgacgctt tttgcggatc gcggacaata
    60541 gccattaaga tcaattgcaa aaaggccagc gttaacagca acccggcggc ggagatgtgg
    60601 cgagaaatcg cgctcaggcc cgtaaacggc agggtgatga taaacatcag ggttagcaat
    60661 gatactaacg gcagcaaccc cgtggcgatt agcgtgaaca acttttgaat agagtcgatt
    60721 aaacgtgact gtgtacgcgc gaggatcacc gccagtgcgg taaccagacc aaatgtaaga
    60781 tacataaacc agtcggttgc aaaaaagagc gtattgaaaa acgtgatgcc aacaagttta
    60841 aacaactcac cccacagtaa aagcaccagc cacgtcaggc cattggagag aaaaatcacc
    60901 agtaatataa gtacgttatg ccatactgac tggtaaaagt agctatagcg gctactgcca
    60961 ttgcggatac gaaggctttg ttgtatccac ggtagcaaca acattgccat caacagcaga
    61021 tagcagccaa aatcccaaat cgccttttca gctctccagg ggttcatgcc atcagtttgc
    61081 cacttcagcc aaccgctcat ccctgtcgtg gcaataaaca ccagcgccag ccatccccaa
    61141 aggcgcttct gtttaaaaga gatcacggag aagagtaaaa cggatgaaaa cgcgacggtc
    61201 gcaggcacgc cataaacaat ccaactgtga ttttttccgg caagccaggc gataagcagg
    61261 tagcaaacca gcccctgaag taatccggta gcaatcatcc cctgaagtaa tccggtagca
    61321 atcatccccc agcgtgttgc cggtgaaagt tcaacgttat ccatgttggt tatccagaat
    61381 caaaaggtgg gttaattatc gcatccgggc agtagtattt tgcttttttc agaaaataat
    61441 caaaaaaagt tagcgtggtg aatcgatact ttaccggttg aatttgcatc aatttcattc
    61501 aggaatgcga ttccactcac aatattcccg ccatataaac caagatttaa ccttttgaga
    61561 acattttcca cacctaaaat gctatttctg cgataatagc aaccgtttcg tgacaggaat
    61621 cacggagttt tttgtcaaat atgaatttct ccagatacgt aaatctatga gccttgtcgc
    61681 ggttaacacc cccaaaaaga ctttactatt caggcaatac atattggcta aggagcagtg
    61741 aaatgcgcgt taacaatggt ttgcccccgc aagaactcga ggcttatggt atcagtgacg
    61801 tacatgatat cgtttacaac ccaagctacg acctgctgta tcaggaagag ctcgatccga
    61861 gcctgacagg ttatgagcgc ggggtgttaa ctaatctggg tgccgttgcc gtcgataccg
    61921 ggatcttcac cggtcgttca ccaaaagata agtatatcgt ccgtgacgat accactcgcg
    61981 atactttctg gtgggcagac aaaggcaaag gtaagaacga caacaaacct ctctctccgg
    62041 aaacctggca gcatctgaaa ggcctggtga ccaggcagct ttccggcaaa cgtctgttcg
    62101 ttgtcgacgc tttctgtggt gcgaacccgg atactcgtct ttccgtccgt ttcatcaccg
    62161 aagtggcctg gcaggcgcat tttgtcaaaa acatgtttat tcgcccgagc gatgaagagc
    62221 tggcaggttt caaaccagac tttatcgtta tgaacggcgc gaagtgcact aacccgcagt
    62281 ggaaagaaca gggtctgaac tccgaaaact tcgtggcgtt taacctgacc gagcgcatgc
    62341 agctgattgg cggcacctgg tacggcggcg aaatgaagaa agggatgttc tcgatgatga
    62401 actacctgct gccgctgaaa ggtatcgctt ctatgcactg ctccgccaac gttggcgaga
    62461 aaggcgatgt tgcggtgttc ttcggccttt ccggcaccgg taaaaccacc ctttccaccg
    62521 acccgaaacg ccgcctgatt ggcgatgacg aacacggctg ggacgatgac ggcgtgttta
    62581 atttcgaagg cggctgctac gcaaaaacta tcaagctgtc gaaagaagcg gaacctgaaa
    62641 tctacaacgc tatccgtcgt gatgcgctgc tggaaaacgt caccgtgcgt gaagatggca
    62701 ctatcgactt tgatgatggt tcaaaaaccg agaacacccg cgtttcttat ccaatctatc
    62761 acatcgataa catcgttaag ccggtttcca aagcgggcca cgcgactaag gttatcttcc
    62821 tgactgctga tgctttcggc gtgttgccgc cggtttctcg cctgactgcc gatcaaaccc
    62881 agtatcactt cctctctggc ttcaccgcca aactggccgg tactgagcgt ggcatcaccg
    62941 aaccgacgcc aaccttctcc gcttgcttcg gcgcggcgtt cctgtcgctg cacccgactc
    63001 agtacgcaga agtgctggtg aaacgtatgc aggcggcggg cgcgcaggct tatctggtta
    63061 acactggctg gaacggcact ggcaaacgta tctcgattaa agatacccgc gccattatcg
    63121 acgccatcct caacggttcg ctggataatg cagaaacctt cactctgccg atgtttaacc
    63181 tggcgatccc aaccgaactg ccgggcgtag acacgaagat tctcgatccg cgtaacacct
    63241 acgcttctcc ggaacagtgg caggaaaaag ccgaaaccct ggcgaaactg tttatcgaca
    63301 acttcgataa atacaccgac acccctgcgg gtgccgcgct ggtagcggct ggtccgaaac
    63361 tgtaatgatt tgaagctgga gaatatctat ccagtatctt atagaaagca aaacgggagg
    63421 caccttcgcc tcccgtttat ttacccttct tttgtcgtgc cctgcgcccg cgttaccggc
    63481 actggcagcc aggcgcgaat ggaaagcccg ccccgctcgc tggtgccaag ctccagcatc
    63541 ccgttatggt tatccacgat acgctgcaca attgccagcc ctaatcccgt gccgctaatg
    63601 gtgcgcgcac tgtcgccgcg gacaaacggc tggaacaggt gcttacgttg ttccggcgca
    63661 attcccggac cgtcatcttc cacctggaac caggcgcgat tcggctccgt tccgctgctg
    63721 actttgatcc agccattgcc ataacgggcg gcgttgacca ccatattcgc caccgcgcgt
    63781 ttgatcgaca gcgggtgcat tttcacttca atgctgccgg ggtaaagcgc ggtttcaatt
    63841 tcccgctcat agccactttc ggcagcaatc acctcaccga gcactgcatt aagatccgcc
    63901 atttccatcg gcatctcctg cccggtgcgc aggtagtcga taaactgctc aatgatggcg
    63961 ttgcactctt cgatatcttt attgatcgat tctgccagat agccatcctg ctcgctcatc
    64021 atctcagtcg ccaggcgaat acgcgtcagc ggcgtgcgca agtcgtgact tacccccgcc
    64081 atcagcagcg tgcggtcatc cgccagttgc ttaacaccag ccgccatatg gttaaaggca
    64141 cgggtaacgg aacgcacctc cgaagcgcca tattcacgca gcggcggcgg aataatccct
    64201 ttaccaacct gcaaggctgc gtgttcgaga tcgaccaacg gtcggttctg gatacgaata
    64261 aacagccacg ccccgcctat cgccaatagc ataatcgcca gcgtatagcg gaacagcgga
    64321 gagaaatcgc cctgatgaat ttcggtcagc ggcacgcgta cccagatatt gggcgacagc
    64381 caggttttca gccagacgac aggcgaactt ttgttgacct caacgcgcac ttccgtcggg
    64441 ccgcccagtt gctgtgccat ctgatggctt aagaattcat agtgttgcgc ccaacgcaga
    64501 cctgcctctt cggcagcctc gttggagtag agagagatcc ccagctcacg gtagatctcc
    64561 cgacggaaag cgggaggcac aaccaactgc gtgccgtcct ccagttgcag tttgtcggtc
    64621 atcaacatac gcacttcgta cgcgaggact ttattaaact gctggaggct cggcaaaatc
    64681 gcgaagttca gcaccaccag ataagtcgtc accaggctgg cgaacagcaa ggtgacgatg
    64741 agcaataacg tacgggcaaa tgaacttcgt ggcgagaagc gcaatcgcct catgctttag
    64801 agccgtccgg tacaaagacg tagcccaggc cccaaacggt ctgaatgtaa cgcggatgcg
    64861 ctggatcttc ttctaccatg cggcgcagac gcgaaatctg cacgtcgatg gagcgttcca
    64921 ttgcggaata ttcacgacca cgggcaaggt tcatcagctt atcgcgggag agcggctcac
    64981 gcggatggct gaccagtgcc ttcagtaccg caaactcacc gctggtgagc ggcatcggct
    65041 catcttcgcg gaacatttcg cgcgtaccga ggttaagttt gaacttaccg aaagcaatta
    65101 ccgcctcttc ctgtgacggt gcgcctggca gttcgttcgc ctgacgacgc agcaccgcac
    65161 gaatacgggc cagcagttca cgcgggttaa acggttttgg aatgtagtcg tcagcgccaa
    65221 tctccaggcc tacgatacgg tccacttctt cccctttcgc cgtcaccata atgatcggca
    65281 tcgggttgct ctgactacga agacgtcggc aaatcgacaa gccatcttca ccaggtaaca
    65341 ttaaatccag taccataaga tggaaagatt cacgagtcag caggcgatcc atctgttctg
    65401 cattagcgac gcttcgaacc tggaagcctt gttcggtgag ataacgttcc agcagcgcac
    65461 gcaggcgcat gtcgtcatcg accaccagaa tcttgtagtt ctcttgcatt gtttgtactc
    65521 ccaaaggttc gcaacaattt gtaagcgtgt attcttaaaa aagctcacgt tcgtcaccag
    65581 ctaaatctgg tatgaatttc agcctaaatt gttacaaagc atattaaaca gcagcttaag
    65641 tatacaattt attcggcgaa acattattga ttctgttgat atgatcacgt tatacccaat
    65701 gtgcgcatta tcaaacagac aaagggaatc aacgagatga aaacgcccct ggttacccgg
    65761 gaagggtatg aaaaactcaa acaagagctt aattatctct ggcgtgaaga acgcccggag
    65821 gtcacaaaaa aggtgacctg ggccgcaagt ctgggcgacc gcagcgaaaa tgctgactat
    65881 cagtataata aaaagcgtct gcgtgaaatc gaccgtcgcg tgcgctatct cactaaatgc
    65941 ctggaaaatc tcaaaatcgt cgattactcc cctcagcagg aaggcaaagt cttttttggc
    66001 gcgtgggtgg agattgaaaa cgacgatggc gtgactcacc gtttccgtat tgtcggctac
    66061 gatgaaattt ttggccgtaa agattacatc tctatcgatt ccccgatggc ccgcgcattg
    66121 ctgaaaaaag aagtcggcga tctggcggtg gtgaataccc ctgccgggga agcgaactgg
    66181 tatgttaatg ctatcgagta cgtgaaaccg taaggaagag tcttaacctc ctgccgatgg
    66241 ctggcatttt tgccagccag tccgtataac tatcccctga tttttgatcc gaaaagatga
    66301 actcaaacca tgatgaatga ttcgttctgc cgcattattg cgggtgaaat tcaggcgcgc
    66361 ccggaacagg ttgacgctgc cgttcgcctg cttgacgaag ggaataccgt gccgtttatc
    66421 gcacgttatc gtaaggaaat caccggcggt ctggatgaca cgcagctgcg taatctggaa
    66481 acgcgtctga gctatctgcg cgagctggaa gagagacgtc aggcgatcct caaatccatt
    66541 tccgagcaag gcaaactcac cgatgatctg gcgaaggcca tcaacgccac cctaagcaaa
    66601 accgaactcg aagacctcta cctgccctac aaacctaaac gccgcacccg cgggcaaatc
    66661 gccattgaag cagggcttga gccgttggct gacctgctgt ggagcgatcc gtcacacacg
    66721 ccagaagtcg ccgctgcaca atatgttgat gccgataaag gcgtggcaga taccaaagcc
    66781 gcgctggatg gcgcgcgcta tatcctgatg gaacggtttg ccgaagatgc cacgctgctg
    66841 gcgaaagtgc gtgattatct gtggaagaac gcgcatttgg tttctacggt ggtgagcggt
    66901 aaagaagagg aaggggcgaa attccgcgac tatttcgatc atcacgaacc gttgtccacg
    66961 gtgccttctc accgcgcgct ggcgatgttc cgtgggcgta acgaaggcgt actccagctt
    67021 tcgctgaatg ccgatccgca gttcgatgag ccgcccaaag agagctattg cgagcaaatc
    67081 atcatggatc accttggcct gcgcctgaac aatgccccgg cggatagctg gcgcaaaagc
    67141 gtagtgagct ggacctggcg catcaaggtg ctgatgcatc tggaaaccga actgatgggt
    67201 accgtgcgcg aacgcgcgga agatgaagca atcaacgtct ttgcccgtaa cctgcacgat
    67261 ctgctgatgg cggcccctgc cggactgcgt gcaacgatgg gcctcgatcc gggtctgcgt
    67321 accggggtaa aagtggcggt ggtcgatgcc actggcaaac tggtggcgac cgataccatt
    67381 tacccgcaca ccggacaagc cgcaaaagca gcgatgaccg ttgctgcctt gtgtgaaaaa
    67441 cataacgttg aactggtggc gatcggcaac ggtacagctt cccgcgaaac tgaacgtttc
    67501 tatctcgacg tgcagaagca gttcccgaaa gtgaccgcac agaaagtgat cgtcagcgaa
    67561 gctggcgcgt cggtttactc agcttccgag ctggcagcgc aggagttccc ggatctcgac
    67621 gtttcgctgc gtggtgcggt atctatcgcc cgccgtttgc aggatccgct ggcggagctg
    67681 gtgaaaatcg atccgaaatc tatcggcgta ggtcagtatc agcatgacgt cagccagacg
    67741 caactggccc gcaaactgga tgcagtagta gaagactgcg taaacgccgt tggcgtcgat
    67801 ctcaacaccg cttctgttcc gctattaacc cgcgtggcgg gcctgacgcg catgatggcg
    67861 caaaacatcg tggcctggcg cgatgagaac ggccagttcc agaaccgtca gcaactgctg
    67921 aaagtcagcc gtctggggcc gaaagccttc gagcagtgcg cgggcttcct gcgcattaac
    67981 cacggtgata acccgctgga cgcctctacc gttcatccgg aagcctatcc ggtggtggaa
    68041 cgcattctgg cagcaacaca gcaggcactg aaagatctga tgggtaacag cagcgaactg
    68101 cgtaacctga aagcgtctga ctttactgat gaaaaattcg gtgtgccgac agtaactgac
    68161 atcatcaaag agctggaaaa accgggtcgc gatccgcgtc cggaatttaa aaccgctcag
    68221 tttgccgatg gcgtcgagac aatgaacgac ctgcaaccgg gtatgatcct cgaaggcgca
    68281 gtgaccaacg tcaccaactt tggcgcgttt gtcgatattg gcgtgcatca ggacggcctg
    68341 gttcacatct cttcattgtc gaacaagttt gtggaagatc cgcataccgt ggtgaaagcg
    68401 ggcgacattg tgaaggtgaa agtgctggaa gtggatcttc agcgtaaacg tatcgccctg
    68461 actatgcgtc tggacgagca gcctggcgaa accaactctc gtcgcggcgg cggtaatgaa
    68521 cgcccgcaaa acaaccgccc ggcagccaaa ccacgcggtc gtgaagcgca gcctgccggt
    68581 aatagcgcga tgatggatgc gctggcggca gcgatgggta aaaaacgtta aacgcccgga
    68641 ctggccccta aacctgcgcg ataaggcgtt cacgccgcat ctggcaacga acgctttgcc
    68701 tgacataaaa gtgccggaga atatctccgg cacttttatt ccacagccaa actcacaata
    68761 tactccgtta atatttatca cttcattaac aactgaaacc ttaattaaac attagccagt
    68821 ccgggtaact cactattcga attatatttt cgctgcgata taaccttgag ccacatcaac
    68881 attgagtcag attattattc aaaccaacat tcgcacacat tttaagtatt gctgatagaa
    68941 accattctca ttatcattgt gttgttgatt atttaatctc tcctttgttg gcaaatcatc
    69001 tggtctcatg tcgcggtcaa acgccccatg aggtagttat ccagttaatg agaaacaagt
    69061 aggcacctat gcaatacact ccagatactg cgtggaaaat cactggcttt tcccgtgaaa
    69121 tcagcccggc atatcgccaa aaactgcttt ctctaggcat gttacctggc tcctcttttg
    69181 atgtggtgcg cgtcgctcca ctcggcgacc ccattcatat cgaaacccgt cgtgtgagcc
    69241 tggtattacg caaaaaagat ctggccttat tagaagtgga agcggtttcc tgttaatacg
    69301 gtgataacaa caatgaaaaa attaaccatt ggcttaattg gtaatccaaa ttctggcaag
    69361 acaaccttat ttaaccagct cactggcgca cgtcagcgtg taggtaactg ggctggcgtt
    69421 accgtcgaac gtaaagaagg gcaattctcc accaccgatc atcaggtcac gctggtggac
    69481 ctgcccggca cctattctct gaccaccatc tcatcgcaga cctcgctcga tgagcaaatc
    69541 gcctgtcact acattttgag tggcgacgcc gacctgctga ttaacgtggt ggatgcgtct
    69601 aaccttgagc gtaacctgta cctgacgctg caactgctgg aactcggcat tccctgcatc
    69661 gtggcgctga acatgctcga cattgccgag aagcaaaata ttcgtattga aattgatgct
    69721 ctatcggcac gtcttggctg cccggtgatc ccgctggttt caacccgtgg tcgcggtatt
    69781 gaagcgctca agctggcgat tgatcgctat aaagctaacg agaatgtgga actggtgcat
    69841 tacgcacagc cgctactcaa cgaagcagat tcactggcaa aagtgatgcc ttccgacatc
    69901 ccgctgaaac aacgtcgctg gctgggcctg caaatgctgg aaggcgatat ctacagccgc
    69961 gcctacgccg gtgaagcgtc gcagcatctg gatgccaccc tcgcccgtct gcgtaatgaa
    70021 atggatgatc cggcgctgca cattgccgat gcgcgttacc agtgcattgc tgccatctgt
    70081 gatgtggtaa gcaacaccct gacggcagaa cccagccgtt tcaccactgc ggtagataaa
    70141 atcgtgctca accgtttcct cggtctgccg attttcctct ttgtgatgta cctgatgttc
    70201 ctgctggcta tcaacatcgg cggggcgtta cagccgctgt ttaacgtcgg ctccgtggcg
    70261 ctatttgtgc atggtattca atggattggc tacacgctcc acttcccgga ctggctgact
    70321 atcttcctcg cccagggcct gggtggcggt attaacaccg tgctgccact ggtgccgcag
    70381 atcggcatga tgtacctgtt cctctccttc cttgaggact ccggctatat ggcgcgtgca
    70441 gcgtttgtga tggaccgcct gatgcaggcg ctgggcttgc cggggaaatc ctttgtgccg
    70501 ctgatcgtcg gtttcggttg taacgtaccg tcggtaatgg gcgcacgtac gcttgatgca
    70561 ccgcgtgaac gtctgatgac catcatgatg gcaccgttta tgtcctgcgg cgcgcgtctg
    70621 gctatcttcg cagtattcgc ggctgccttc ttcgggcaga acggtgccct ggcggttttc
    70681 tcgctgtata tgctcggtat cgtgatggcg gtgctgactg gcctgatgct caagtacacc
    70741 atcatgcgcg gtgaagcgac gccgtttgtc atggagctgc cggtctacca tgtaccacac
    70801 gttaaaagcc tgattatcca gacctggcag cgtctgaagg gcttcgttct gcgtgctggt
    70861 aaagtgatta tcatcgtcag cattttcctg agcgctttca acagcttctc gttgagcggg
    70921 aaaatcgtcg ataacatcaa cgactcggcg ctggcttccg tcagccgggt gataacccct
    70981 gtcttcaagc cgattggcgt acatgaagat aactggcagg caacggttgg cctgtttacg
    71041 ggcgcaatgg caaaagaagt ggtggtgggt acgctcaaca ccctctacac cgcagaaaat
    71101 attcaggacg aagagttcaa tccggcggaa ttcaacctcg gtgaagagct gttcagtgcg
    71161 gtagatgaaa cctggcagag cctgaaagac accttcagcc ttagcgtact gatgaacccc
    71221 attgaagcta gcaaaggcaa cggtgaaatg ggtaccgggg cgatgggcgt gatggatcag
    71281 aaattcggta gcgcagctgc cgcatacagc tacctgattt tcgtcctgct gtatgtacca
    71341 tgtatctcgg tgatgggagc catcgcccgt gaatcaagcc gtggctggat gggcttctcc
    71401 atcctgtggg ggctgaatat cgcttactca ctggcaacat tgttctatca ggtcgccagc
    71461 tacagtcagc acccaactta cagcctggtg tgcattctgg cggtaatcct gtttaacatc
    71521 gtagctatcg gtctgctgcg ccgcgcgcgt agccgggtgg atatcgaact gctggcaacc
    71581 cgcaagtcgg taagcagttg ctgcgcagcc agcaccaccg gtgattgcca ttaatggctt
    71641 cacttattca ggtacgtgat ttgctggcgt tacggggccg tatggaagcg acccagataa
    71701 gccagacatt gaacactccg cagccaatga ttaacgccat gctgcaacag ctggaaagca
    71761 tgggcaaagc cgtgcggatt caggaagaac ctgacggctg cctctctggc agttgtaaaa
    71821 gctgcccgga aggcaaagcc tgtctgcgcg agtggtgggc gctgcgttaa ccatactcca
    71881 tcgcctgttt tgaaaagccc ggtatgcgtt tgcatccggg cttttttgcg tgcggctttc
    71941 cataaaaatg caattcttgc agcgcaacgt aagttcctct gaaagcgtct cgcagggatg
    72001 aaaactcgct aatgcacaga tgtggagtgg cgcgtagagt cgcggcattc aaacaacagg
    72061 tgaaggaacg ccatgagtaa aaagcagagt tccaccccac atgatgcgct gttcaaactc
    72121 tttttacgcc aaccggagac ggcacgcgat tttcttgcgt ttcatttacc ggcacccatt
    72181 cacgcgctct gtgatatgaa aaccctcaag ctggagtcga ggagctttat tgatgacgat
    72241 ctgcgtgaaa gctattccga tgtgctgtgg tcggtgaaaa cggaacaagg accaggatac
    72301 atctattgtc tgattgaaca tcaaagcacc tcaaacaaac tgatcgcatt tcgcatgatg
    72361 cgttacgcta ttgccgcaat gcaaaatcac cttgatgctg gatacaaaac gttgccgatg
    72421 gtggtgccat tgttgtttta ccacggtatt gaaagcccct atccctattc gctgtgttgg
    72481 ctggattgtt tcgccgatcc caaactggca aggcagcttt atgcctccgc atttccgctg
    72541 attgatgtca ccgtcatgcc tgatgatgaa atcatgcagc accgacgtat ggcgctgctg
    72601 gagttaattc aaaaacatat tcgtcaacgc gatctgatgg ggctggtaga gcaaatggcc
    72661 tgcttattaa gtagtggata cgctaatgac agacaaatca aagggctgtt taattacata
    72721 ctgcaaactg gcgacgctgt acgttttaac gattttatcg acggcgttgc cgaacgttca
    72781 ccgaaacaca aggagagttt aatgactatt gcggaaagat tgcggcagga gggggaacaa
    72841 tccaaagccc tgcatatagc caaaataatg cttgaatccg gagtccctct tgcagacatc
    72901 atgcgattta ccgggctgtc agaagaagag ttggctgcgg cgagtagact ggccccctga
    72961 atctccagac aaccagtatc a
//
LOCUS       DAJTUP010000004        71408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTUP010000004 DAJTUP010000000
VERSION     DAJTUP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.004"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(868..2454)
                     /locus_tag="OO468_000271"
     CDS             complement(868..2454)
                     /locus_tag="OO468_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR6257523.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(2809..4980)
                     /gene="glcB"
                     /locus_tag="OO468_000272"
     CDS             complement(2809..4980)
                     /gene="glcB"
                     /locus_tag="OO468_000272"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR6257524.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5002..5406)
                     /locus_tag="OO468_000273"
     CDS             complement(5002..5406)
                     /locus_tag="OO468_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR6257525.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5411..6630)
                     /gene="glcF"
                     /locus_tag="OO468_000274"
                     /pseudo
     CDS             complement(5411..6630)
                     /gene="glcF"
                     /locus_tag="OO468_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6641..7693)
                     /gene="glcE"
                     /locus_tag="OO468_000275"
     CDS             complement(6641..7693)
                     /gene="glcE"
                     /locus_tag="OO468_000275"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR6257526.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7693..9192)
                     /gene="glcD"
                     /locus_tag="OO468_000276"
                     /pseudo
     CDS             complement(7693..9192)
                     /gene="glcD"
                     /locus_tag="OO468_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9443..10213
                     /gene="glcC"
                     /locus_tag="OO468_000277"
     CDS             9443..10213
                     /gene="glcC"
                     /locus_tag="OO468_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR6257527.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10337..11478)
                     /locus_tag="OO468_000278"
                     /pseudo
     CDS             complement(10337..11478)
                     /locus_tag="OO468_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            11836..13566
                     /locus_tag="OO468_000279"
     CDS             11836..13566
                     /locus_tag="OO468_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR6257528.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13563..14477
                     /locus_tag="OO468_000280"
     CDS             13563..14477
                     /locus_tag="OO468_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR6257529.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14510..14758
                     /locus_tag="OO468_000281"
     CDS             14510..14758
                     /locus_tag="OO468_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6257530.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            14758..15929
                     /locus_tag="OO468_000282"
                     /pseudo
     CDS             14758..15929
                     /locus_tag="OO468_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(15965..17044)
                     /gene="lptG"
                     /locus_tag="OO468_000283"
     CDS             complement(15965..17044)
                     /gene="lptG"
                     /locus_tag="OO468_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6257531.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17041..18111)
                     /gene="lptF"
                     /locus_tag="OO468_000284"
     CDS             complement(17041..18111)
                     /gene="lptF"
                     /locus_tag="OO468_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6257532.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18142..18702)
                     /locus_tag="OO468_000285"
     CDS             complement(18142..18702)
                     /locus_tag="OO468_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6257533.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18714..19538)
                     /locus_tag="OO468_000286"
     CDS             complement(18714..19538)
                     /locus_tag="OO468_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257534.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19538..20887)
                     /locus_tag="OO468_000287"
     CDS             complement(19538..20887)
                     /locus_tag="OO468_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR6257535.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(20933..21691)
                     /locus_tag="OO468_000288"
     CDS             complement(20933..21691)
                     /locus_tag="OO468_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257536.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21723..22436)
                     /locus_tag="OO468_000289"
     CDS             complement(21723..22436)
                     /locus_tag="OO468_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6257537.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22610..23302
                     /locus_tag="OO468_000290"
     CDS             22610..23302
                     /locus_tag="OO468_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR6257538.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23351..24850)
                     /gene="pitB"
                     /locus_tag="OO468_000291"
     CDS             complement(23351..24850)
                     /gene="pitB"
                     /locus_tag="OO468_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR6257539.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25142..27001)
                     /gene="gss"
                     /locus_tag="OO468_000292"
     CDS             complement(25142..27001)
                     /gene="gss"
                     /locus_tag="OO468_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR6257540.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27206..28072
                     /gene="yghU"
                     /locus_tag="OO468_000293"
     CDS             27206..28072
                     /gene="yghU"
                     /locus_tag="OO468_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR6257541.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28195..28443)
                     /gene="hybG"
                     /locus_tag="OO468_000294"
     CDS             complement(28195..28443)
                     /gene="hybG"
                     /locus_tag="OO468_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR6257542.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28577..28918)
                     /gene="hypA"
                     /locus_tag="OO468_000295"
     CDS             complement(28577..28918)
                     /gene="hypA"
                     /locus_tag="OO468_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6257543.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(28911..29399)
                     /gene="hybE"
                     /locus_tag="OO468_000296"
     CDS             complement(28911..29399)
                     /gene="hybE"
                     /locus_tag="OO468_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR6257544.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29392..29886)
                     /locus_tag="OO468_000297"
     CDS             complement(29392..29886)
                     /locus_tag="OO468_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR6257545.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(29886..31589)
                     /gene="hybC"
                     /locus_tag="OO468_000298"
     CDS             complement(29886..31589)
                     /gene="hybC"
                     /locus_tag="OO468_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR6257546.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31586..32764)
                     /gene="hybB"
                     /locus_tag="OO468_000299"
     CDS             complement(31586..32764)
                     /gene="hybB"
                     /locus_tag="OO468_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR6257547.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(32754..33740)
                     /gene="hybA"
                     /locus_tag="OO468_000300"
     CDS             complement(32754..33740)
                     /gene="hybA"
                     /locus_tag="OO468_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR6257548.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(33743..34861)
                     /gene="hybO"
                     /locus_tag="OO468_000301"
     CDS             complement(33743..34861)
                     /gene="hybO"
                     /locus_tag="OO468_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR6257549.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35050..35337)
                     /locus_tag="OO468_000302"
     CDS             complement(35050..35337)
                     /locus_tag="OO468_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR6257550.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35456..36343)
                     /locus_tag="OO468_000303"
     CDS             complement(35456..36343)
                     /locus_tag="OO468_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6257551.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36500..37540
                     /gene="gpr"
                     /locus_tag="OO468_000304"
     CDS             36500..37540
                     /gene="gpr"
                     /locus_tag="OO468_000304"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR6257552.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37580..38074)
                     /locus_tag="OO468_000305"
     CDS             complement(37580..38074)
                     /locus_tag="OO468_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR6257553.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38265..39149
                     /gene="yghA"
                     /locus_tag="OO468_000306"
     CDS             38265..39149
                     /gene="yghA"
                     /locus_tag="OO468_000306"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6257554.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39421..39846)
                     /gene="exbD"
                     /locus_tag="OO468_000307"
     CDS             complement(39421..39846)
                     /gene="exbD"
                     /locus_tag="OO468_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR6257555.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(39853..40587)
                     /gene="exbB"
                     /locus_tag="OO468_000308"
     CDS             complement(39853..40587)
                     /gene="exbB"
                     /locus_tag="OO468_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6257556.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            40839..42026
                     /gene="metC"
                     /locus_tag="OO468_000309"
     CDS             40839..42026
                     /gene="metC"
                     /locus_tag="OO468_000309"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR6257557.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42166..42825
                     /locus_tag="OO468_000310"
     CDS             42166..42825
                     /locus_tag="OO468_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6257558.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(42865..43764)
                     /gene="yqhC"
                     /locus_tag="OO468_000311"
     CDS             complement(42865..43764)
                     /gene="yqhC"
                     /locus_tag="OO468_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR6257559.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            43958..45121
                     /gene="yqhD"
                     /locus_tag="OO468_000312"
     CDS             43958..45121
                     /gene="yqhD"
                     /locus_tag="OO468_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR6257560.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45226..46053
                     /gene="dkgA"
                     /locus_tag="OO468_000313"
     CDS             45226..46053
                     /gene="dkgA"
                     /locus_tag="OO468_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR6257561.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46253..47179
                     /locus_tag="OO468_000314"
     CDS             46253..47179
                     /locus_tag="OO468_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR6257562.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47230..47487
                     /gene="yqhH"
                     /locus_tag="OO468_000315"
     CDS             47230..47487
                     /gene="yqhH"
                     /locus_tag="OO468_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR6257563.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47530..49749)
                     /locus_tag="OO468_000316"
     CDS             complement(47530..49749)
                     /locus_tag="OO468_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR6257564.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(49860..51272)
                     /gene="ftsP"
                     /locus_tag="OO468_000317"
     CDS             complement(49860..51272)
                     /gene="ftsP"
                     /locus_tag="OO468_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR6257565.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51347..52084)
                     /gene="plsC"
                     /locus_tag="OO468_000318"
                     /gene_synonym="parF"
     CDS             complement(51347..52084)
                     /gene="plsC"
                     /locus_tag="OO468_000318"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR6257566.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52318..54576)
                     /gene="parC"
                     /locus_tag="OO468_000319"
     CDS             complement(52318..54576)
                     /gene="parC"
                     /locus_tag="OO468_000319"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR6257567.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55122..55604)
                     /locus_tag="OO468_000320"
     CDS             complement(55122..55604)
                     /locus_tag="OO468_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR6257568.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55657..56049)
                     /gene="ygiW"
                     /locus_tag="OO468_000321"
     CDS             complement(55657..56049)
                     /gene="ygiW"
                     /locus_tag="OO468_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR6257569.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56201..56860
                     /gene="qseB"
                     /locus_tag="OO468_000322"
     CDS             56201..56860
                     /gene="qseB"
                     /locus_tag="OO468_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR6257570.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            56857..58131
                     /gene="qseC"
                     /locus_tag="OO468_000323"
     CDS             56857..58131
                     /gene="qseC"
                     /locus_tag="OO468_000323"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR6257571.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58177..58509)
                     /locus_tag="OO468_000324"
     CDS             complement(58177..58509)
                     /locus_tag="OO468_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR6257572.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            58828..59409
                     /gene="mdaB"
                     /locus_tag="OO468_000325"
     CDS             58828..59409
                     /gene="mdaB"
                     /locus_tag="OO468_000325"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR6257573.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59440..59754
                     /locus_tag="OO468_000326"
     CDS             59440..59754
                     /locus_tag="OO468_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR6257574.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(59802..61694)
                     /gene="parE"
                     /locus_tag="OO468_000327"
     CDS             complement(59802..61694)
                     /gene="parE"
                     /locus_tag="OO468_000327"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR6257575.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(61723..62304)
                     /gene="yqiA"
                     /locus_tag="OO468_000328"
     CDS             complement(61723..62304)
                     /gene="yqiA"
                     /locus_tag="OO468_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR6257576.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62304..63131)
                     /gene="cpdA"
                     /locus_tag="OO468_000329"
     CDS             complement(62304..63131)
                     /gene="cpdA"
                     /locus_tag="OO468_000329"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR6257577.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63156..63578)
                     /locus_tag="OO468_000330"
     CDS             complement(63156..63578)
                     /locus_tag="OO468_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR6257578.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63579..64208)
                     /gene="nudF"
                     /locus_tag="OO468_000331"
     CDS             complement(63579..64208)
                     /gene="nudF"
                     /locus_tag="OO468_000331"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR6257579.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64413..65894
                     /gene="tolC"
                     /locus_tag="OO468_000332"
     CDS             64413..65894
                     /gene="tolC"
                     /locus_tag="OO468_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR6257580.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66042..66713
                     /locus_tag="OO468_000333"
     CDS             66042..66713
                     /locus_tag="OO468_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR6257581.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            66719..67879
                     /locus_tag="OO468_000334"
     CDS             66719..67879
                     /locus_tag="OO468_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6257582.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(67917..68705)
                     /gene="ygiD"
                     /locus_tag="OO468_000335"
     CDS             complement(67917..68705)
                     /gene="ygiD"
                     /locus_tag="OO468_000335"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR6257583.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            68848..69621
                     /gene="zupT"
                     /locus_tag="OO468_000336"
     CDS             68848..69621
                     /gene="zupT"
                     /locus_tag="OO468_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR6257584.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69679..69849)
                     /locus_tag="OO468_000337"
     CDS             complement(69679..69849)
                     /locus_tag="OO468_000337"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257585.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70110..70763)
                     /gene="ribB"
                     /locus_tag="OO468_000338"
     CDS             complement(70110..70763)
                     /gene="ribB"
                     /locus_tag="OO468_000338"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR6257586.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(70867..71015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71137..>71408
                     /gene="ubiK"
                     /locus_tag="OO468_000339"
     CDS             71137..>71408
                     /gene="ubiK"
                     /locus_tag="OO468_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR6257587.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTCL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPET"
BASE COUNT        17809 a        19034 c        17652 g        16913 t
ORIGIN      
        1 cagggctatt tttagcagga aatactcagg tgtcaacatt cgaactccaa ctctgaaata
       61 ttcatataag ttaatgattt taatgatttt aatgatttta atgattttaa tgattttttc
      121 atttattcac acctaataaa agtattcaaa ttcagatgat ttttctgctc aacagacaca
      181 aacaggcaat aatactctcc aaagcgaaca atatttggca ttacgattta atgcttcata
      241 atcaccaaat tatttaacca taaattttct tatttctaac aaaatgattg gtgtgttgtt
      301 ggagttaaaa tacatacata atattaacat ctgccaacat ttcactttcc gcaagacgga
      361 tgatttcgaa tcctcatcca cgttacccta tcccaaatat ctttattaat aggcagtaac
      421 gaacactgtt atagcaagat taacttatta acctaacggc atcttttatg taacaagtgg
      481 agataaattg cttaaaattg atcctcgaca aatcttaaaa gttctcatta ttctgcaaaa
      541 tcagctttgc gaataatctt atgtttttgg gagttttggg aattttgatc tactcattgt
      601 tctagtgaaa aatataaata agagaaaaaa cgccattcgt catgaaagca acagatgaag
      661 caatgtaata acatactaaa attgatctca tgaaagtggg tactttataa ccagcacaga
      721 agccaagttg tataaatatt aaacaattcg catcgtaaaa cggagactac gtataaagaa
      781 gccaatcgta aaaacggttc cctggcgaga taaacgacag acaaatggaa agccgggtga
      841 tatttacctt catccggctt tattgattta cgagactaac atcccggtaa acacatacgc
      901 ctgcagcagg gtgataacgc tggcaaaaat cagactgtgc ttcacggtgt agcggaacag
      961 ttcagattct cggcccacca tgcccgtcgc ggcgcaggcc acggcgatag attgcgggga
     1021 gatcatcttg ccagttacgc cgccgctggt atttgctgcc accagcagag tgtcagagac
     1081 gttgatttgc tgcgccgtgg tcgattgcag tgaaccaaac agggcgtttg aagaagtgtc
     1141 cgacccggta aggaatacgc ccagccagcc gagaaacggt gagaaaaacg ggaacatcac
     1201 gcctgtacct gccagtacca gcgccagcgt ggtggacatg ccggaatagt tggtgacgaa
     1261 ggcgaatgcc agcaccatgc caatcgacaa tatcggccac ttcagactaa ttagcatttc
     1321 ggcaaagacg ccaatccctt tcttgatgcc cacgccgagg atgaagatag agataatcgc
     1381 cgcaataaaa atagcggtgc cgccagccga gagagggtcg aatttaaaca ccgcatccat
     1441 tggcgttggt tgggcgacaa tgggtgccgc tttcaacact tgttgatgca aatgagggat
     1501 ctggaaatta atcaccagtg aataaaacgc gccgcccgga gcaaataacg ctttaaacgg
     1561 cttcatggtc cagatggtga ccagcaccgt taagattaaa aacggtgacc acgcacgaat
     1621 gatttgcccc agactatatt ctgaaggcac gggaccgcca gaagatggct tatttaccac
     1681 catcgcacct gcggattgcc ccatgctgat tgccgtttcg gtatttttcg gccgccagac
     1741 tttaaggaat aaagcgagtg agacgatact caccagcgcc gaagtaatat ccggcagttc
     1801 cggaccaata tagttagagg taaagaactg agtgacagcg aagcttcccc cagcaaccag
     1861 cgccgctggc cacgtctctt tcaccccttt ccagccgtcc atcattgcta ccagccagaa
     1921 cggcacaaga accgacagga acggtaactg acgtcccgcc attgcgccaa tgtggaacgg
     1981 atcgattccc gttacctgac cggcgaccag aatcggcacg cccaacgcac caaacgccac
     2041 cgtcgcacca aagccagcag ctccttccag caacgcacca aaggagaaac caatcagtaa
     2101 cacctgcaaa cgctgatcgt cggtgatgga gataaccgag ctgcggataa tatcgaactg
     2161 cccgctggca acggttaatt tatacaggaa caccgccgcg acaataatcc acgcaattgg
     2221 ccataatcca taaataaagc catagcccgc agcagcaaat gccatatcaa tcggcatttt
     2281 aaaggcgaat atcgcaatca ggatagataa tataagggtt attgctccag caacatgtcc
     2341 tttcagacgt aataccgcga gtgcaacgaa gaagaatatt atcgggatca gggcgaccag
     2401 agcggatagc cccagtcctc ccatcggcat atacatttgg gtccaggtaa ccatattgtt
     2461 cagtctctta ttatctttat atgcttgata tacttaaggt tgtaataagc aaaagaggac
     2521 tgaactgtaa aatataggcg ttatacttta cagcaacagt acgccgctaa cgcaattgct
     2581 acctctggca taacaagtat atcgggtaag ggtttctgtt ccgcacacgc aggcgcagag
     2641 tatcgttaag atgtccatat tgttgtttta ggcccgctag taatgcgcta cgggtattta
     2701 atattgttaa accctgataa tcgctccggt tatttccggg ataaatgtac taccgcagtt
     2761 actatcatag ccccgacaat aaaacttgcc ggggcttttt tgacgctatt aatgactttc
     2821 tttttcgcgt aaacgccagg cgtgtaataa cggttcggta taaccgtttg gctgtttcac
     2881 gccgaggaag attaaatcgc tggcagcttt aaaagcacac gagttagcga aattccccgc
     2941 catcggacga taagccggat cgccagcatt ttgctgatca accactttcg ccatattctc
     3001 cagcgacgcc tgcacctgtt ctttggtcag aataccgtga cgtaaccagt tggcgatatg
     3061 ctggctggag atacgcagcg ttgcgcggtc ttccatcagc gccacgttgt gaatatccgg
     3121 cacttttgaa caaccaatcc cctgctccac ccaacgcacc acgtacccca gaatcccctg
     3181 cacgttgtta tccagctctt gttggatctc ttgcgccgac cagttagcgt tttcagccac
     3241 cggaatagtc agcagatcgt ccagcagcgg ttcaaattca gcactgaact cggtctgggc
     3301 aatgttggct tgcacgctct gtacgttggt atggtggtag tgcagcgcat ggagcgtagc
     3361 agcggttggt gacggaaccc aggctgtgtt tgccccggca cgcagttggt cgcccttctg
     3421 gctgtacatg tctgccatca ggtccggcat tgcccacatg cctttaccga tttgcgcttt
     3481 accgcgcagc ccacagaaca gaccggaaag cacgttatta cgttcgtagg ctttgatcca
     3541 cggcgtcgat ttcatctggt ttttacgcag catcgggcca gcttccatca ccgaatgcat
     3601 ttcatcgccg gtacggtcga ggaaaccggt attgatgaac gccacgcggt tgcgcgcctg
     3661 agcgatacag ctacgcaagt tcagcgaggt ccggcgttct tcatccataa tgcccatttt
     3721 cagggtattc ggtgccatac cgagcattgt ctcaatgcgg gtaaacagtt tgttggcgaa
     3781 cgccacttcc tgcggaccgt gcattttcgg tttcacaata tagacgctgc cagtgcgcga
     3841 gtttttctgc acttttaaat catagagggc aatcgcgcca gtcatgacgc catcaagaat
     3901 accttccggg atttcattgc cttcgctgtc ccaaatcaca ggaatggtca tcaaatgacc
     3961 cacgttgcgg ataaacagca acgagcgtcc gtgcagagaa atttcagagc catcggcggc
     4021 ggtgtactga cgatcgtcat tcagtttacg tacgatttgc cgaccgtttt tctccatttt
     4081 ctcttgcaga gtcccctgca tcaggcccag cagattgcgg tacagcagga ttttatcttc
     4141 cgcatcaacc gccgcgaccg aatcttcgca gtcgagaatg gtactgatag cagcttcgac
     4201 gataacatcg ttgatgtgcg ccggatcgtc tttgccaatc cgcccgttgg catcaatttg
     4261 cagttcaata tgcaggccgt tatttttcag caaaatgcag gtcggcgcag cggcatcgcc
     4321 acggtaaccg acaaactgtg ctggagtacg taacgtggtt tctttaccat ttttcaactg
     4381 gatgcgtaat tgtttatcga ccaccttaaa cgccaccaca tcctgatagc tgccgttttc
     4441 cagcggtagt gattcatcga ggaaacgtcg aacccaggcg ataacctgct caccgcgctg
     4501 cggatcgtag ccactgacca tcgccccttc ctgcgggatg atgtcgctgc cgtataacgc
     4561 atcgtacagt gagccccagc gagcgttcgc cgcgttcagc gcgtagcggg cgttcattgc
     4621 cggaaccacc agctgtggcc ccgcctgact ggtgatttcg ctgtcaatgc ccgtggtttc
     4681 caccgtcacg cgctccggtt gcggcaccag gtagcccagt tcacgaagga aagatttata
     4741 ggccgcttta tcttttaccg gccccggatt gctgcgatgc cactcatcaa gcgccgcctg
     4801 aatgcgatcg cgttctgcca gcaactgacg attttctggt gccagatcgt gaacgatctc
     4861 atcaacattg tgccagaatg cagcagcgtc cagttccacg cccggcaaaa cttctccatc
     4921 cacaaaacgt ttaaaattgg cgtcaatgcg taaacggccc tgggttatgg tttgactcat
     4981 tgtttctctc ctcgttttcg cttatttcgc caacaccgct gctgccactt tcgcgacctg
     5041 cgcatcctgt gctccggtta aaccagaaac gcccacggca ccaataattt gcccatcaac
     5101 aacaaccggt acgccgcctt ccagcgacgt cagtaacggc gcagtcacga acgcggtacg
     5161 tccgttgttc accatctctt catagccctt agtttcacga cgccccagcg cggcggtacg
     5221 cgctttctcc tgggagatgt aagccgcaat cggcgcgcaa tcgtccatgc gacttaacgc
     5281 cagcagatga ccgccgtcat cggcaacagc aatggaaaca gaccagttat ttttctgcgc
     5341 ctcttcctga cctgcggcaa taattgcact cgccatttgc tggctaagaa tgactttagt
     5401 tttcattttt ttattccttt tcaagggctt gttctacaat ttcaatccag tgacgcacag
     5461 aggtacgacc ggcgctcgcc agatgcgtct ggcaaccaat gttggcggtg acgatcattt
     5521 ccggtttgcc gctttccagc gcattcattt tgttatcccg cagctggcgt gccagatcgg
     5581 gatgcgttaa cgcatatgtt cccgctgaac cgcagcacag atggctgtcg ggaacgtccg
     5641 ttaaggtaaa tccaagacga agtaacactt tttccacttc gccgttcagc ttttgcgcat
     5701 gttgtagggt acacgggcag tggaaggcca gttttttatc gccgcgaatt gccagttttt
     5761 ccagcggttc ctcgcgcaga agttcgacta aatcgaccgc cagttcactg acctgacgcg
     5821 ctttatcggc atataacgca tcgtttttca gcatctgccc atactctttg acgaacgcgc
     5881 cgcaaccgct ggcggtttgc aaaattgcct cggcacctgc ttcaatcgcg ggccaccagg
     5941 catcaatatt attgcgcgcc cgtgccaacc ctttctcctg cgcattaaga tgatagtcca
     6001 ccgcgccaca acagcctgct tcgttagctg acatgacgct gatccccaga cgatccagca
     6061 ctcgcgcagt tgccgcgttg gtgttgggcg aaagcgtagg ctgggcgcag ccttccagca
     6121 ttaaaacccg acgcttatgg cgcagcggcg gacgcggttt agctttcacc gtttcagcag
     6181 gcagttttgc tctgacctgt tccggtaaaa acggtcgcag caccagccct acctgcgtca
     6241 gcgcacggaa gaccgccgga cgcggcacta cctggcgcaa tccttcgcgc agcattcgct
     6301 ccggcagtgg gcgtttcact ttctgctcga caatatcacg cccgatatcc agcaaattgt
     6361 gatagcgcac accagaagga caggtggttt cacaattgcg gcaagtgagg cagcgatcga
     6421 gatgctcctg tgttttaagc gtgacttcgt tgccttccag cacctgttta atcagataga
     6481 tgcgcccgcg cggcccgtcc agttcatcgc ccagaagctg ataggttggg caggttgcgg
     6541 tacaaaatcc gcagtgaaca caggcgcaca ggatgctgtc ggcttccagc gcgcgttctg
     6601 ccgcatctct tcggttaatc gggtttgcat agcctgctcc tcaaagttcc gcgtacatgc
     6661 gaccggggtt aaacacgccg caagggtcga gctgctgttt aagctgctgg tgatagcgga
     6721 ataaaggagc cgatagcggg gcaaagccac catctccggc actaaagcgg gtcgcatgac
     6781 cgccagcgtt gcgggcgatg cgatggattt gattgtcatc ggctgtcgat ttcagccagc
     6841 gtaacgcccc gccccagtcg atcagttgct cgccgggtaa atccatcatc ggcgcatcgc
     6901 tgggtaatga aatgcgccat aaggtacctg gtaacgagaa gaacggcagt tgttgttcac
     6961 gcaattgctg ccagaactga cccacaacct cttcgccacc cagcagttca cgcgctgctt
     7021 ttaccgatcc ttcgccgccc tcaaggcgga tccacaacgc attgtcgaag taacataagc
     7081 cactaatggg taatggctgg agttgccact cggcgatttc actcatggct tcttgcaggc
     7141 tgatttcccg acgcaggctc agagaggcac gcggtcgcgg taacactttc attgagattt
     7201 cagtgagcac gccaagacaa ccgtagcttc cggccattaa ccgtgagaga tcgtatccgg
     7261 caacgttttt catcacttcg ccaccaaaac gcagatgttt tccagtgccg gtaatgatgc
     7321 gcgtgccgag gacaaaatcg cggaccgaac cgctccacgg gcgacgcggc cccgccagcc
     7381 cgcaggcgac catcccgccc caggtggctt cttcaccata atgcggcggc tcgcagggaa
     7441 gcatttgccc ggcgctctcc agcgccgctt caatcgccac cagcggcgtt ccggcacgag
     7501 cggttatcac cagctcggtc gggtcgtaat taacaatgcc gcgatgacaa cgcacatcca
     7561 gcgtttgccc ggtgactggg cgacctaaaa aggctttgct attgctgccc tgaatcacca
     7621 gcggcgtttt atcgctaatt gcctgattca cctgctccag cagcgcctga ctgtaatcac
     7681 actcgcgtag catcaaaagc gctccagttc aggaaaaggt aaatgaccgt gatgcacatg
     7741 catggcacca aattcagcac agcggtgtag cgtgggaatg tttttcccag ggttcagcaa
     7801 accatcgggg tcaaacgccg ccttgaccgc atggaaggtc gtgatttcat cgctgttgaa
     7861 ctgggcgcac atttgattga ttttttctcg cccgatgcca tgttccccac tgatgctgcc
     7921 gccaacttca acgcagagtt cgaggatctt cccgcccagc tcttccgcgc gggcaaattc
     7981 accaggttcg ttggcatcga aaaggattaa cgggtgcatg ttgccatctc cggcatgaaa
     8041 gacgttggca acacgtaaat catattgctg cgataaacgg gcaatgcctt ccagtacgcc
     8101 aggcagggcg cgacgcggga tggtgccatc catgcagtag taatccgggg agatacgtcc
     8161 taccgccggg aacgcatttt tgcgaccggc ccagaaacgt acgcgctctg cttcgtcctg
     8221 tgccaaacgg acgtcagtcg cgcccgcttt caacaagatg tagttaaccc gctcgcagtc
     8281 ttcctgtacg tcagactcca cgccgtccag ttcgcataac aaaatcgctt cggcgtcgac
     8341 gggataaccg gcatgaataa aatcttccgc cgcgcggatc gacaggttat ccatcatctc
     8401 cagcccgccg gggataatgc cattggcgat gatgtcacca accgcaagtc cggctttttc
     8461 taccgagtca aagctggcta acagaacccg cgccacgggc ggcttcggca gcagttttac
     8521 cgtcacttcg gtggtcacgc cgagcatacc ttccgatccg gtgaacagcg ccagcaggtc
     8581 aaaaccaggt gaatccagcg cgtccgatcc cagcgtcagc gcctcgccgt ccagcgtttg
     8641 cacttcaatt ttcagcaggt tatgtacggt cagaccatat ttcaggcagt ggacgccacc
     8701 ggcattttcc gccacattgc cgccaattga acaggcgatt tgtgaggaag gatccggcgc
     8761 gtaatagaga ttatgcggcg caacggccta ggagatcgcc aggttacata cgcccggctg
     8821 cacgcgcgcg cggcgaccaa cggggttaat gtcgaggctc tctttaaagc gcgccatcac
     8881 caacaacaca cctttttcca gcggcagcgc gccaccagaa agcccggtgc ctgcaccacg
     8941 ggtcaccacc ggtacacgca ggcgatggca gacagccaga atcgctgtca cctgttccat
     9001 ttgcttaggc agaacaacca gtaatggacg cgtgcgatac gcgctcaacc cgtcacactc
     9061 gtaaggaatg atctcctcat cggtatgcag gatctcaagt ccagggacat gctcacgcag
     9121 tgccatcagt accgatgtgc ggtcgacatc gggtaaagcg ccatcaagac gctcttcgta
     9181 caagatgctc atgagtaggc ttcgctttgt tgtgttgtgt ggcagctgat ttttgcgcgc
     9241 tgcttctgtg aacagttatt aagcgggctt ttcgttttcg tctatctctt tagctaccgg
     9301 tcagaccatt ttttttccag ctctgtgacc ttgtcttggt taacttaatg ttaaattgat
     9361 gtaacataat cacttgcgtg atgtgcgtgt tttgcgagtt aagaacagaa aaattggtcc
     9421 tacctgtgca cgaggtccgg gaatgaaaga tgaacgtcgc cctatttgcg aagtggttgc
     9481 agagagtatc gaacggttaa ttatcgacgg cgtactgaag gtcggtcagc cgcttccctc
     9541 ggaacgtcga ctgtgtgaaa agctcggctt ctcacgctcc gcactgcgtg aagggctgac
     9601 cgtgctgcgc gggcgcggga ttattgaaac ggcgcagggt cgcgattctc atgtcgcacg
     9661 gcttaatcgg gagcaggaca ccagcccgct gatccatctg ttcagtacgc agccgcgaac
     9721 gctgtacgat ctgctcgacg ttcgcgcatt actggagggc gaatcggcaa ggctggcggc
     9781 aacgctggga acgcaggctg attttgttgt gataacccgc tgttatgaaa aaatgctcgc
     9841 cgccagtgag aaccacaaag agatttcgct gatcgaacat gcgcagttgg atcacgcttt
     9901 ccatctcgcc atttgtcagg cttctcacaa tcaggtgctg gtgtttacgc tgcaatcatt
     9961 gaccgatctg atgtttaatt cagtgtttgc cagcgtaaat aatctctacc atcgaccaca
    10021 gcaaaaaaag cagatcgatc gccagcatgc gcggatctac aacgcggtgt tgcagcggct
    10081 gccgcacgtc gctcagcgcg cagcgcgcga tcatgtacgg accgtgaaaa agaatctcca
    10141 cgatatcgag ctggaaggcc accatttgat tcgctcggcg gtgccgctgg agatgaacaa
    10201 ggtgggaatg tgagcagagt tttaccctat ccccaaggcg aggggaccgc ccctgcactt
    10261 aacaggatct ggtgttaaat ctgttcatct ataccctctc ccgaatgacc gcccctgcgc
    10321 taaacaggat ctggtattaa atttgctttt catacaatcg gtaacgcttg tacggctccg
    10381 ccccaatgcg ttccagcatg ttattcatgc ctgtattggt ttcgaggatc catgacatct
    10441 ccagcgcatc gatcttccgg cgggcaaacg gatcgcgtaa ggcttcaatc aataacagcg
    10501 caatcaccgg gccgatgcgg ctgaactgat actcgtcgcg cacgcccatc agcggtactc
    10561 gcgcagttcg cacaccgctg actttcaaac gccacagcaa ttttgcccag ccgaagggaa
    10621 agagcgatcc gttcagatcg gcaatcgcct cgttgatgtt aggcaagcca caataaacgc
    10681 gcagggtgca gaatcaatct cagcgatgta gatcatatcg tccggcacca gatatttaag
    10741 ttggtcgccc atggtcgcga attcatgttc ggtaaacggc acaaatcccc agttgtgctg
    10801 ccagccagag ttgaaaatct cacgcaggat ctgcatctct tcggcaaacc gctgacgatt
    10861 gatgcagcga atggtcacct ttttgcgcac ctgatccatc agttttttta gcgccggaga
    10921 gaaagtgaga tcggttcgct gcatccacca cgccagtaaa tcaatgcctt tgtgataacc
    10981 cagttgttca atatgcgcgg cataccacgg tttgccgtgt ggcatcatcg cacagggtgg
    11041 tgtgtcaaaa ccttcaatca gtaatccgct ttcctgattg atattcaggc tgaaaggccc
    11101 gctgatctta cttgcacctt gtgacttcaa ccacgcttcc gctgcgccaa acaacgcggc
    11161 aaaatcctgc ggatcatcaa tggcgtcaat catgccgaaa tgaccggtat cttcaccgta
    11221 acgctcacgg tgcaaggtat ctatttgcgc ggtaatacgc ccaactatct gccccgcttt
    11281 ttttgccacc cacgcctgcc agatgatatg gtccgtcccc ggatttttcg cagacaaatg
    11341 ctcgttgcgt tcaatgaata aagggggtat ccagtttgga tcgtcgggat aaagtgatga
    11401 cggaaaagcg ataaatgcct taaggtcatt tttattaatt actttttcaa ttttaatcat
    11461 cagcagatcg cattccatat ctctgtggat aacaaataat taatggtgaa aaacaggttg
    11521 caacagcaac gagcggcatc ttaataatca actcaatgaa tacaaacgac gaataaatag
    11581 tattaattaa tgattatgat aaggtcagag gctacacaga aacgcctcaa tgccttttgt
    11641 caatttaagg cgaccggaca aggcttcttc ttaatatatt cataaattaa atatcattca
    11701 taataaaaaa aacttaaact cattatttaa atcaatgttc attttcacac cgatgaaaat
    11761 actatctcat ttatctttta tgcctatata atccaccgca ttattgtcat tttatttttg
    11821 atatccaatt ttttcgtggt gtatatgtct aataaaatct ttacgcattc cctacctatg
    11881 cgctatgccg attttccaac gctggttgat gctttggact acgccgctct gagtagagcc
    11941 ggaatgaatt tttatgacag acgttgccaa cttgaagatc aactggaata tcagacatta
    12001 aaaacgcgtg ccgaagctgg tgcgaagcgg ttgttatcgc tgaacctgaa aaaaggcgaa
    12061 cgcgtggcgc tgattgccga aacgagtagc gggtttgtag aggctttttt tgcctgtcag
    12121 tatgctggct tagtcgccgt cccgttggcg attccaatgg gcgtcggtca gcgggattcc
    12181 tggagcgcca aactccaggg tttactggca agttgccagc ccgcagccat tatcactggt
    12241 gatgagtggt tgccactggt caatgccgcg acgcataaca accccgaatt acatgtttta
    12301 agccacgctt ggtttaaggc attaccggaa gccgatgttg cgctccagcg tccagttccg
    12361 aacgatatcg cctacctcca gtacacctcc ggcagcaccc gttttccccg tggcgtcatt
    12421 atcacccatc gcgaagtgat ggctaatcta cgtgctataa gccacgatgg cattaaatta
    12481 cgccctagcg accgctgcgt ctcctggcag cctttctacc atgatatggg actggtcggc
    12541 tttctcctgc cccccgtcgc cacgcaactt tcagtagatt atttgcgcac tcaggatttt
    12601 gccatgcgtc ctttgcaatg gcttaaattg atcagtaaaa atcgcggcac cgtttccgtt
    12661 gcgccgccgt ttggctatga attgtgccaa cgccgcgtga atgaaaaaga tctcgctgaa
    12721 ctggatcttt cctgctggcg cgtcgctggt attggtgctg agccgatctc cgcagaacaa
    12781 ctccatcaat tcgctgaatg tttccgtcag gttaactttg acaataaaac tttcatgccg
    12841 tgctacggac tggcagaaaa tgcgctggct gtcagcttct ctgatgaagc ctccggggtt
    12901 gtggttaacg aagtggatcg cgacatcctc gaatatcagg gtaaagccgt cgcgccgggt
    12961 gcagagacac gcgccgtatc gactttcgtc aactgcggca aagcgttgcc ggaacatggt
    13021 attgaaatcc gcaatgaagc aggtatgccg gtcgcggaac gtgtggtagg ccatatttgc
    13081 atctccggtc ccagtctgat gagcggttac tttggcgacc aggtttcgca agacgagatt
    13141 gccgcgacgg gctggttaga caccggcgac ctcggttatc tgctggacgg ttatctgtat
    13201 gtcaccggac gcattaaaga tctgattatt attcgtggcc gtaatatctg gccgcaggat
    13261 attgaatata tagcggaaca ggaaccggaa attcattctg gcgatgcgat tgcttttgtt
    13321 accgcccagg aaaaaatcat tttgcagatc cagtgtcgga tcagcgacga agaacgtcgc
    13381 gggcagctta tccacgcgct ggcggcacgg atccaaagcg aatttggcgt gaccgcggct
    13441 atcgatctgt tgccgcccca cagtattccc cgaacgtcct ccggcaagcc tgcccgtgcg
    13501 gaagcgaaaa aacgttatca gaaggcttat gctgccagtc ttaatgtgca ggaatccctg
    13561 gcataaatca aactgtcgcg gtgacgggcg ctaccgggtt tatcggtaaa tatattatcg
    13621 ataacctgct cgcccgcggc tttcacgttc gcgcattgac gcgtactgcc cgcgctcacg
    13681 tcaatgataa tctcacctgg gtgcgcggtt cgctggaaga tactcattca cttagcgagc
    13741 tggttgccgg agccagcgta gtggtccatt gcgccgggca agtgcgcggg cacaaagaag
    13801 agattttcac ccgctgtaac gttgacggca gcctgcgcct gatgcaagca gcaaaagaga
    13861 gcggcttttg ccaacgtttt ctgtttatct cttcgctggc ggcgcgccat cccgagctct
    13921 cctggtatgc aaaatccaaa tacgtcgccg agcaacgact ggcagcaatg gcggacgaaa
    13981 ttacgctggg cgtttttcgc ccgacagccg tgtatggtcc cggcgataaa gagttaaaac
    14041 cgctgtttga ctggatgcta cgcggcctgc tgccacgact tggtgcgcca gatacacaac
    14101 tctcttttct tcacgtcacc gattttgcgc aagcagtggg tcagtggtta agcgccgaaa
    14161 ctgtacagac gcaaacctat gaattatgcg atggcgtcgc cggtggctat gactggcaac
    14221 gcgtacgaca gcttgccgcc aaagcccgtt gtggttccgt gcgaatggtt gacattcctc
    14281 tgccggtact cacaggcctt gcggatatca gtaccgcgtt gagtcgcctg gcaggtaaag
    14341 aacctatgct gacccgctcg aaaattcgtg aattaaccca cgccgactgg tcggcaagta
    14401 ataaccgtat ttctgaagat atcaattggt ttcccgggat tagcctggaa cacgcattac
    14461 gcaacgggct attttgattg tcttgtttta aaggtgtgtt aacgtttcta tggtaaatcg
    14521 tgaaatagta atggattaca tcctttcctg tttacaggat ttggtcgaaa acggtgtgga
    14581 aattaagccc gacagcgatc tcgtcaacga tcttggcctg gagtcgatca aagtgatgga
    14641 tctcttaatg atgctggaag acagattcga tatttccatt cctattaata ttctgctgga
    14701 tgtcaaaacc cctgcgcaat taatggaaac tctacttcca tggctggaga ataaataatg
    14761 gggctatacg ataaatatgc gcgccttgct ggcgagcgtc tgcaatttag tgacaacggg
    14821 ttaacaccgt ttggcacctg tattgatgaa gtgtattccg ccaccgaagg ccggatcggc
    14881 aataaaaaag tgattctggc cgggaccaat aattatctcg gtttaacctt taatcatgat
    14941 gccatcagcg aaggtcaggc cgcgttagcg gcccaaggca ccggcaccac cggttctcgt
    15001 atggcaaacg gcagttatgc gccgcacctg gcgttagaga aagagattgc cgagtttttc
    15061 aatcgcccta ccgctatcgt ttttcaaccg gttataccgc taatttaggc gtcatcagcg
    15121 cacttgccga ccataacgcc gtggtgttac tggatgccga cagccacgcc agtatttatg
    15181 acgtctgttc tctcggtggc gcggaaatta ttcgtttccg ccataacgat gctaaagatc
    15241 tggaacggcg catggttcgc ctcggtgaac gggcgaaaga ggcgattatc atcgttgagg
    15301 gcatctacag catgctcggt gacgttgcgc cgctggcgga aattgttgat atcaaacgtc
    15361 gtctcggtgg ttatttgatt gtcgatgaag cgcattcgtt tggcgtgtta ggcgcaacgg
    15421 ggcgcggact ggctgaagct gtcggcgttg aagatgatgt ggatattatc gttggtactt
    15481 tcagtaaaag tctggcctcg attggcggtt ttgctgtcgt ttcggaagcg atggaagtgc
    15541 tgcgctacgg cagccgcccg tatattttta ccgcctctcc ttctccgtcc tgcattgcga
    15601 ccgtgcgctc gtcgttaaga actattgcca cacaaccaga gctgcggcaa aagctgatgg
    15661 ataacgccaa ccatctgtat gacggtctgc aaaaactggg ttatgagtta agttcacata
    15721 ttagcccggt agtgccagta ataatcggtt cgaaagagga aggtttacgc atctggcgcg
    15781 agcttatctc gccgggcgtg tatgtgaatc ttatcctgcc gcctgccgcg cctgcaggca
    15841 tcacgctgct gcgctgtagc gtaaatgctg cccacagtcg tgagcaaatc gatgccatca
    15901 ttcaggcatt tgctactctg aagcagtaat gtgagcccgc cgtggtgttt gcgccacggc
    15961 gggtttaatg ttgtctgtca taccaataca ccaggctcaa cgccaccaac acaaacgcta
    16021 ccggtaaacc gagcgcggta acaggtgcgc ttaacgcaaa tagcaatccc agattaacca
    16081 tgatttgata gctgatccag gtgagtaagc ccacgatgac gcctacagcg agacggctac
    16141 ccatcccagg cgaacgcggg gcactaaagg taaacggcac agccagcaaa atcatcgcca
    16201 gggtcaggat gggttgcccc agtttttccc acagtgcaag gtggtattcg ctgctgggtt
    16261 gcccggtttc ctgcaaataa tggatgtaat gattaagctg acggacagaa aaagtattcc
    16321 ccggcattga taactcttca agattcatac tggtgaaggc cgattgccag gcaagattat
    16381 ctaatgtttc cagcgtttct ttgccattca gccatttttt atgattcaca ccatgcagga
    16441 cccacgtttt gtcgtcttca atggtggcac tgcgtgcata aatgtaggat tcaagcgaga
    16501 gatcgtcgcg ataatgaaat atctccacgc ccaccggctg gccctgctcg ttcagcgatt
    16561 tcaccgtcac aaattcatta ccacgcctgg cccacagcat attgccggta atgtcatcgt
    16621 cctcccccaa cgcggtggcg gtcgatttga tttgcaacgc ctgttgctgc aatggcgacg
    16681 ccacccactc atctatcgcg cctaacgaaa cagtccacaa tatcccggca accagcgcca
    16741 ccagtgcaat acggaagata gaaaaccccg tgctgcgaat ggcggtaagc tcactgtttt
    16801 tcgataactg ccccagtcca acaataccac ccaataacgc gataaacggc gaaagttcga
    16861 ccagtgtgcg cggtaaggtc attagcacca ccagcaccgc ctgagtccag cgataaccgc
    16921 ccgggctgac accatccagt tcgttaatca ggttgaaggt ggtaaaaagc ggcagcaata
    16981 gccctgcagc ggcggcaaaa ccgagaaaga gatgacggat taaatagcga ctgaaaacat
    17041 tcatcgggaa aatttctgca gcaaagagaa gtcacgtgcg accagtatca gcaggcccat
    17101 tagcatgagt ccgggcacta accacaaacc aggaatgagg ggaatcgcgc cgttagccac
    17161 cagtgtacgg cagacgtcgc cgccataaaa aatggcaata aacagcaacg tcagtggcaa
    17221 taacgttgaa aatcgccctt gccgcggttt tacccgactt aatgaaatgg ccagcagcgc
    17281 catcaacaat gcggtcaggc cacggctttg tcgccattgc agttcggcat gatcggcagg
    17341 aaaggcggag cgcgccagct ccgtgaccga tttcgctttg cgtttgacgt tcgggctttg
    17401 atccagcgga ttcagatgta attgcagatt acggtagatc tgttcgttgt cgtcacgccc
    17461 ctgatgatcg agaagatagg cagtgccgtt gtgcaacata acggtcggtt tctcgggtga
    17521 tgggtcaacc acatcaaccg aaccggcgcg gaaaatacgg gttcgattgg cagtagaagt
    17581 ataaatcagc gcgtcagtca ggcgattatt atcctgatca accgtctgcg aaaggatcat
    17641 tcgtccgtta tcgttagtgt taaatttctt tgcccgcaac tggcgaacat ccagctccga
    17701 ctgcgactgc tgctccagtt gataaatctg cgcataagcc cacggacgac catacatcga
    17761 cagaagggtg acaaaaatac ttagcggtat cgccagataa agaacggctt tgtacaagcg
    17821 tcccggactg ccccccgccg cagagatagc ggtaatttcc gagtcggtat acatctgccc
    17881 tagcgtcacg ccaactgaca catacagacc aacaggtaac aacatctcca gtgcaatcag
    17941 tactttgtag aaaacgatat ccagcacaac atcaagagct aatgtcccat ttgccgcttc
    18001 ggtcagataa cgctgtgcgg agtagctggc gaaaataaag atcagaaaac cgacgataat
    18061 cagcaccaga cggcgcgtac cacgcatgat gtagtgttca accagcttca tctcactaac
    18121 gccctcaatc ctttgagttt tttattcatt gatcagtttc tgcgccaacg caatgacatg
    18181 atctacctct tccggcgtca ggctgccgtc ctttgcccac tgaatttgcc cggctttatt
    18241 cagcaccaga atggttgaac tctgctccgg caagcgccag gcaacgcgtc cctgtccatt
    18301 gccgtcaata acgaattgcg cccacggata gcggcgttta tttttttcga ttttgccaac
    18361 aacaaaatag ccagtaccaa agatggcatc atcggtgttc acaatggtgg tgggctgaaa
    18421 acgatcctgc ggaaagtttg ccgcttcaac cgctttaatt aacagtgagt tctttttctt
    18481 cgccgactta cgcccggcga tatactgaat aatgcggact tttcccgcta attgtgtgct
    18541 gttccacttt tggtagtgaa tttcgtcgtc gctattcaga agcagctcgc cgccttcctg
    18601 gatataaaca ggggtaaccg ttttgccagt gacgaaattg tgtgcctgca cacccggggc
    18661 taagagcatg ataatcaacg caataatcaa acgagatgac atcattgctc cggctatagc
    18721 ggaaaacgcg aaatcagcag ctctaagcgc tgacaaatat cgctatccag cgccaacggt
    18781 gcataatacg actggcggtt ttccatatca tccgccacca tgtcgtcgtg tttatctccc
    18841 atcaggccat taatggcggc gaggaaacgg gaaatctgta acgcctcttt attttcagga
    18901 tcaagcgatc ttaaggtgtc gtaataagaa acctgctggc tgatgatttt tcgcacatca
    18961 tgaaattccc tgccggttag tcgcggtttg gcaataatct ctttcagggt atgcatttgc
    19021 ttttgccgat aggcaataaa actgcttacg ctttctaact ggctatggcg taatgaatag
    19081 ttcaccaacg accagaccgg agagcttaaa taaatccgta acaggttgcc gtaatacgac
    19141 acaatatttt tcttaccgtt acgaaagccg tcctgaaaac gccccagaaa aacggtcgtt
    19201 ttgccgaata aaaatccggc ctgatgcttc tttaaataca gccgttgcgc aaaacgcagg
    19261 tgcttgtagc gcgctcgcat gtatttaaat tgtttgcgct catcagcagt cagctcatcg
    19321 cctttggctt gtttgcgaac taaacgcagt aattcctgac gatcaacgcc attgagccag
    19381 aattgcaggc ataacgcgta attatcatga atgatttcgg tggtacaact tagtgcaata
    19441 gtcggcggca aactaacctg tgcatcaacg acatcatctt cttcaatcgc aacaaataga
    19501 cgctcgctcg cctcaagcgg gaaatatttt ctcatcatca ggatttctct ccggctgcgt
    19561 tataaagcaa ggaatggcgc tgaaaaaatg cctttaaggt tggaattaac gagagcgcca
    19621 cgtccagaca ttgcccaccg aagtaagcaa acgctgctcc cagcacacca aacagatgac
    19681 agaacatgaa cagcaatacg atgtagccga ttgaggcgat ggtttgcgcc acgagaaaag
    19741 cgcgctgttt tcccgccatc aatagcaatg actcctgcgg gaagcccagc atcgaaatca
    19801 caattgcgcc cagcatcacc tgaatcaggt catacgcttc gagatactta acgccaaaca
    19861 ccagcgaaat gagcggcttg ccgacgatca acaccgcgag cgccaccaga ataccgattc
    19921 cgcccgccag taaaccggac ttcacaccta acaaccacgg tctggtggtg cgcggatcta
    19981 aacgcatcac ctctgggtaa aaacttttac ccagcaaacc cgctggcgtt ccggcggcgt
    20041 cgaagaatgt catggcgatt ttaaataacc cggcggcagc gggtcctaac acgatcccca
    20101 ccaacactgt gctgcacgag ttacgcgccg accagatgga gtgggcaatg ttggttgacc
    20161 agacaaaact ccacgcgcct ttaatgtgtc gggcagactc aaacagattc aatttgaagg
    20221 cgttatggat atttctacgg cgtaattcgc gcgcggcaaa ccaccagtac atggtgccgc
    20281 caaccagatt cgacacgtac caggcaataa caaaacccac aaaaccaaag tcaaaatacc
    20341 aggctgcgac gctccccgct gcgcgcagaa aaggtttcgt cgcctgctgt acagcaatca
    20401 aatcaaagcg atctaccgca cgcagaatgc cggtcggcgt ggaggaagcc attgaaggaa
    20461 tgagcgtgca atacagcgcc gccagccaaa agctttggtc atccagacct aatgaatggg
    20521 aaaggaatgg cagtaaggca atgccgccga caatcgccac cgcgccgctg acgatatcca
    20581 gcgagaagga aaatgagacg acattgcgaa attgctgcgg attattgttg gtcaaggctg
    20641 gcgttccgta ctgaaccacc agctgccatg tctgaaactt aataaaatcg ctgatcgact
    20701 tggcgtacga ttgcacaatc accagtacac caaacatggc gggcgtcatc cctttaccgg
    20761 cacacgagag cgccaacagc cccagcaagg cgctctcgac attactggag cctaaccagg
    20821 cgctattgcg aataatggtg cgaaacgcgc catctgcaaa ccaatgtttg atgttaaaac
    20881 ccgccaattc agcctgacct tatcgttgat agtaaaaaat atcccgccag ccttaagtta
    20941 aacttcggcg gtcagaaacg atggcaacca gagaaaccgc cttctgtacc tgttccagca
    21001 cttcgctgta gggcgctctg gaatcaatct caagaatttt tgtgccgtta tagccaatcg
    21061 tcgacatgac gccgattttg tcctgcagct cggcatactc atggtcaggc ttgcgggaga
    21121 tggcagtatc aatatcaatc cccaaacgaa tgattaattc aggacggtat tgcgccattt
    21181 tttggtataa actccgttcg cgctgcgcca gaaacatgct gattttcccg gtcgcacgtt
    21241 cgacgccgat ccccggtccg tcataataaa agcccgaaat ttcagcctgc gggaagcgat
    21301 cgctgaccac cagaacgcca ctttgcgcca gtcgctgaac cttgcgtaga ttcgccattc
    21361 ttcgcagcga gaagcagtac ataatcaccg ccgcccatag cgccggagat ttggttttca
    21421 tgctttgggt tttcgaggat ttggccgcca atcgccgttc cagccagacg ccaaccaacg
    21481 gcaatcgttt gattttgtcg ccgtcttcgc cggagagcag ccccagatag cggcgctcgg
    21541 tttgccagtg ttgttgcagc gatttcacca ggtcggtggt cagcgtggat ttaccggtgc
    21601 catcacaccc gaccaccgca atcagccccg gaatgtagtt gggctgcggc gcggttgtac
    21661 tattaacagt ttgtgtttgt agtgcatcca ttcgttaaat catcccctat ccattttggt
    21721 gatcaggaaa gcgcggcgtg aattgcgcgt agtgacgctt gcataatttc atcggctggt
    21781 tgccgaccat ccagttcaag gatcttggcg ccattgaatg tcagttgcgg cgtaacggcg
    21841 attttttcct gtaacgctgc cagttggtgg tcaggtttac gcgcaaacgc ggtttgttca
    21901 tcaatgccaa gacgaatcaa caatacgggt aaataagacg ccatccattg gtacagcttc
    21961 aactcgcgct gccttaacat ttttatccaa ccgttaccgc ccgtggtttt tgccaattgc
    22021 gggccatcaa agcgaaaacc cggcacttca acctgcgggt agcggtcggt gatgagcaga
    22081 tagccttgct ggcttttaca caacattttg cgaaacttgt acgcccgcca gcaggaaagc
    22141 agatagataa ccagcgcagt aatattgcca ggcggagtta agggcttttc gtgtacatgt
    22201 gccgctttac ttcgcagata acacccacaa ggtgcgccaa taacagggag ctgtgaaatc
    22261 cattcgccaa ttcgcccgga cgattgcccg agataaatgt gttctgttgg cattcttgct
    22321 gccagttcat ttaccaggct tgccgtgagg gtcgatttac ctgaaccatc acatcctaca
    22381 atggcaataa cacgcactgg cgtggaatta attattgaca tattacgttg attcacgaaa
    22441 aacccggcag taaataatgt attgaatatt acgtggtccg tgctcagaat atccgttcaa
    22501 ccttattgtg acaatgacta attgtgacat tgacctgaat tacgctttca taaaaacata
    22561 ttaaccaaat aaatattttt aatggatatt taaattaaag gatatattca tgcagtcaat
    22621 aacacctcca ttaattgccg ttattggtag cgatgcttca ggcaagtcaa cggtgtgtga
    22681 acatcttatt accgttgtcg aaaaatatgg tgctgccgaa agagttcatt taggaaaaca
    22741 ggccggaaat gtcggtcgtg cagtgacaaa attacctttg atgggaaaat ccttacataa
    22801 aacaattgaa cgaaatcagg tgaaaacagc aaaaaaattg cctggaccag ttccggcgct
    22861 ggtaattaca gcgtttgtcg cccgtcgctt actgcgcttt cgtcatatgc ttgcctgtcg
    22921 tcgtcgcggg ttaattgttc taaccgaccg ttatcctcag gaccaaattc ctggcgctta
    22981 cgatggtacg gtgttcccac ctaacgttga aggtggtcgt tttgtctcat ggctggcaag
    23041 ccaggaacgt aaagcgtttc actggatggc gagccataag cctgatctgg tcatcaaact
    23101 caatgttgac cttgaagttg cctgtgcacg taaacccgac cataaacggg aatcgctggc
    23161 gaggaagatt gccataacgc cacagttaac ctttggtggt gcacaactgg ttgatatcga
    23221 tgccaatcag ccactggaac aggtgttggt tgatgcagaa aaagcgatta cggattttat
    23281 gaccgcgcgt ggttatcact agtcaaaaat ggaaatgccc gatcgccagg accgggcatt
    23341 ttcaggaagg ttaaatcaac tgcaatgcta tccagtacag cccaccagaa agaaaaattg
    23401 ccgccggtaa agtaaacacc cacgccatca ggatgctggt taccgtttta cgctgtaacc
    23461 caccgccgtc caccaccatc gtccctgcta ccgcagacga gaggacgtgt gttgtggaga
    23521 caggcatccc aatataactg gcaaggccga tagacactgc cgccgtcatt tgtgccgcca
    23581 tgccttgcgc atacgtcatg ccgcgcttac caatcttctc accgatggtc atcgctacac
    23641 gacgccagcc aatcatggtg ccaatgccga gcgccagtgc taccgccatg atgatccaca
    23701 ccggagcgta ctcaatggtg cttaacatat cgctgcgcag ttttttcagc aggttctggt
    23761 cttctttact gacgcctggc agtttcgcca gcttcgcgga ggtatcggag atgcacagca
    23821 taatgcggcg cagctggctg cgctgactca cgcttaacgg ctcgtaactt tccatattgc
    23881 ctggcagcat cgttttaacg cgcgcaatcg catcaaaggt atttgccgga tgacagtgaa
    23941 actctgttac ttgcgtgcca tcagtcgatg ctgcaggcaa tggaggttcc atcgcaatca
    24001 acttctgcgg cagttcagga tgctgttgca ggtagtgttc gaagttggta acggcatcgc
    24061 gggtacgggt aatttcatag ccggacgcat tcatattgac gacgaagcca gcaggggcaa
    24121 tccccaccag taccagcatt accaggccga tccctttctg cccgtcgttc gcgccgtgcg
    24181 aaaacgccac gcccgcagcg gaaacaatca gcgcaatacg cgtccagaat ggcggtttac
    24241 gtttgccctt tttctttttg cgatcttccg gaatgcggtg aatacggtca cgctttttcg
    24301 tcccactcca gaaacggcgc agcaggaaaa tcagaccacc cgcaatgacc aggccgacga
    24361 taggggaaac aatcagcgag gagaaaattt tggttacttc acgcaggttt aacgcatcca
    24421 tcaccgatga gccggttaac agcgcgttgg ttaaaccgat gccgataatc gcaccaatca
    24481 aggtgtgcga actggaggcc ggtaaaccaa agaaccacgt tcccaggttc cagataatcg
    24541 ccgccagcag catggaaaag accatcgcca aaccgtgagt tgaccccata ttcagcaaca
    24601 aatcgattgg taacatatgg acaatggcat aggcaacgct aagtccgccc aataacacgc
    24661 caaaaaagtt aaaaaatgcc gccatcacca cagcaagttg tggctgcatg gcacgagtat
    24721 aaataacggt tgccaccgca ttcgccgtgt catgaaaacc attgattgct tcgtagaaca
    24781 acacaaatgc cagagcaagt aataacaaaa gccccgtata tatatcaagg ccaacaaata
    24841 aatttagcat atattagatt acgccatttt gaatttacga acggacgcat tatcagtgac
    24901 tttaacggca tgggcaaagt gaaatatcat tttttaacga taaaaagagt gttatttctt
    24961 gtgtatcaga caataagcat tcattcaaaa cctttagaaa taatcaaaac acaggtattc
    25021 cactggttaa tatattcaca tatgaaatga ataaatattg gaatatataa atattgaata
    25081 ttttgattaa tccccgtact aattattctt cataatcagt acggggttgc tacaacatgt
    25141 attacttttt caccacaatt aacggttcaa tatcactctc ttttttgatg accagtgatt
    25201 catcaccgcg caaacacgtc ccaccgtagt tgccgccaac ggtgaaggta catacctgaa
    25261 tgtatttacc gtccactttc ggcaaacacc acagttgctg atagatgttt ttctgctcgg
    25321 caaatttacc gctggttttg tccagcacct cttcatgatg gctgaccagg tcgatattgc
    25381 taccacagcg accggcgatc ggtttcactg cgtaacctgt tttcaccaat tcatcattaa
    25441 cagtgaaatc ggtatccagc aggtaacgat ggtgcgggaa cagcgaccag aggatcggca
    25501 gaatcgcttt gttgccgggg atcaccgtcc acagcggctc aaagaccagc acttccgggc
    25561 gcagcaatac gtcgataaga cgcacttcgt tttgcggatg accggtacgg attggcaccg
    25621 cagcaaactc acggtcgcta acttcacgaa tctgatcaaa cgcggtttcc cacgcccagg
    25681 ttttccacac gcagttaacc agtcgccctt ccccatcaat cagttgcccg gcagcatccc
    25741 agcccagttc atccagcccg cgcaagatac gcgtttcaaa gcccgcctgg tgcagcgcct
    25801 gctccataaa ctgcgcgtga tagttttcct cgatatcttt gtcctgcatg atatggacaa
    25861 acggacgtgc acgactgtgt ttccaggcac cagccagttc gttaatcagc ccttccgccg
    25921 gattgaagcc gttgccttta tagccctgct ccgcccaacg ttcgaggatc aagcccgctt
    25981 cggtatgaca ggaggcggag tcggcgttgt attcgtaaac cttcaggcca cgctcatcca
    26041 tgcagaaatc catacgacca gtgatcatat ggtgacggcg acgctgccag gagagacgca
    26101 aacgtggcca gaggattttc ggaatgtcga acagcgccag caggttgtca tctttcagca
    26161 ccttgtcggt tgcgtgaaga tacatcagat gcagctcgtt ggtggcttta attagctcct
    26221 gctcggcact ctcggtaatg gtgtagtaat gatacggatc ctgattgatc acctgaccgt
    26281 tggcctgcac ataggcgttt tgtagcggat ctttttcatc cagccattta ccgtcaaact
    26341 ggcctttgtt ttccagacgc gctccactga ttttcagcag ctcgcctgca atttccggct
    26401 gcggtaagct gtattcagta tcttccgtct ggatcatcca gcccagaatg gtggtgtcat
    26461 caaaagtgtc tttaagggta tagcagccgt tttcgaccac catctccagc tcgcgcgtcc
    26521 actgttgccc ttgcggcaac ggggaatgaa tcacgttctg ttccgcaata cggactttgt
    26581 tgccatgcaa ttgggtaatg atggcgacat ggccagtgtc tttaaattca ccgcctttat
    26641 cccagataag aagcgcaccc gcgaccggcg cacgcggcga gccgttagga aatgcctgca
    26701 atggcaggat gttgtcatta accacttctc gcaggaagcg cagcgagaaa atctcccacg
    26761 ccatacccac gtcagtaaag accacaccgt aattcagaaa gagaaaacgg cgagcaaatt
    26821 caacgcattg ccacttgtgg cccatatatt cgtcgtcgat atagctacgg aatacggcgt
    26881 catcttcgta ttcctgcgga tcgagagaac tgtaatctga agagtagatt gctaccccac
    26941 ctggggcgta gcccaataat gtcccgaacg gggcatcctg gctggtcgtt cctttgctca
    27001 tcactttacc tttaacaata caacctaagc agttggcgta attgttgtgc tctgattacc
    27061 tgctcagcga cattaaccgg acagaggtca gactaatcat acacctcatc gcagcggctg
    27121 gcgcaaaggg ttaaaaaatt cacattctgt acagcaagtg acctgctaca ctgcttcaac
    27181 actaccactc agaaggcaac tcactatgac agacaatact tatcagcccg cgaaagtctg
    27241 gacgtgggat aaatccgctg gcggcgcgtt cgccaatatc aatcgcccgt tttctggtcc
    27301 gacgcatgaa aaaacgctgc ccgttggcaa acatccattg caactttatt cgctgggaac
    27361 gccgaacggt cagaaagtaa cgattatgct tgaggagcta ctggcgctgg gcgttactgg
    27421 tgcagagtac gacgcctggc tgattcgtat tggcgatggc gatcaattct ccagcggctt
    27481 tgtcgaagtg aacccaaact cgaagatccc ggcgctgcgc gatcatacgc ataatccgcc
    27541 gatccgcgtg tttgaatctg gttcgatcct gctttatctg gcggagaaat ttggctactt
    27601 cctgccgcag gatctggcaa aacggactga aacgttgaac tggctgttct ggttacaggg
    27661 tgccgcgccg ttcctcggcg gtggttttgg tcacttttac cattacgcgc cggtgaaaat
    27721 tgagtacgcc atcaaccgct ttaccatgga agccaagcgc ctgctcgacg tgctggataa
    27781 gcaactggcg cagcataagt ttgttgcggg cgatgagtac accattgcgg atatggcgat
    27841 ttggccgtgg tttggcaacg tggtgctcgg cggcgtgtat gatgccgcag agtttctgga
    27901 tgcgggaagt tataaacatg tgcaacgctg ggcgaaagaa gtgggcgaac gtccggcagt
    27961 gaaacgtggg cgtattgtta accgcaccaa cgggccgctg aacgagcagt tgcatgagcg
    28021 ccatgacgcc agtgattttg agacaaatac agaagataag cgtcaggggt aagggttggt
    28081 gttcgtcgca gcaagccatc caggccggat aaggcgttca cgccgcatcc ggcaatcgtg
    28141 cacaatgcct gatgcgacgc tgccgcgtct tatcaggcct acaaaagcta atcatcacgc
    28201 gctggtaatg tcgtaatcca tttggcgcag tgcgtctaat gtggctttgg cttcatcttc
    28261 gtcgatgatg ctcatggcaa atccgacgtg taccagcacc cactggccca gcagatcggc
    28321 agggttacct tcgcaaatca gggcgatatt cacgtcgcgc ttgataccac atacttcaac
    28381 ctgcgcaagc tggtgaatat cttcaccgac agccagcacc tggcctggaa cgccaataca
    28441 cattgttaac tccggttatt tcgattgccg gacaaacgcg aaaaagaacc tttagcgggt
    28501 gttccctctc accctaaccc tctccccgga ggggcgaggg gaccgtccgt gcgcttttta
    28561 ggcgttgtcc tcagtcttat tcaacttcaa tacttttgac gatcagcgaa tcgccggtat
    28621 cgacacgcaa ccgctcgccg tgacagagcg gacactgcgc atcgtgctga tgaatctcca
    28681 ccacctggct gcaatcccag caccaggcct gggcgggttt atagacgata tgtaaatcgc
    28741 acccttgcgc caccgttccg tggcagacaa tttcaaaact aaaacggacg gcgctctcct
    28801 caacgcagga gagcgcgcca atttccagcc acacggcggt gacgcgctta acatcgtgct
    28861 gctccgcctg ccgttggata atttcaacgg cgctctggca aagcgacaac tcatgcattt
    28921 tcgcccctcc tgcggccaaa cagcagggcg cgacgccctg cgtgtggtac atccggattg
    28981 gtgactggca acgaaaggat cattcgtgcg cagtcatccg tcaggcgttg gccttcttca
    29041 atcgacatgc tgtgcgaaag cggcgacatc agcgaacagg agaggtattg cgaaacaccg
    29101 tccagttcgc caacggtaaa ggtcatggtg ccatacggca gttgcagacc aattttttca
    29161 ctcactttgc gcagcggcca gagttgatcc gggccgggga agataactgc actcagcatc
    29221 cacggggtga tcacacaccc cgtccactga ccttcgaaca gcgtaaaatc agaaacatac
    29281 accggcattg aaggatgcag aaaagagaga tcgtgcatcg aacgccgggc aatttcttca
    29341 aacgctgcct gtacttgcgc cttcggggag gtctggaaac ctgctatctc ttcagtcatg
    29401 aatcgcctcc cgtgggatgg cttccacgcc tgattcgcgc agcgcagcca gaacctgctc
    29461 aagcgcaggt tcaatcattg cttcaaccgt cggcgttaag ccgatgtgtg gctccagcga
    29521 ttccgggatc acgccgacca gggtcagctt tttcggaaac tcaccggtga agcgcagggc
    29581 cgacaatacg tcggccaggc caagctgatg cggagagatt ttgttggtaa acaacgccgg
    29641 aacttcttca tcccgcagga tcatcatcgt tcccggagtg ttctttttcg acacaatggc
    29701 atccgcaata atcagatgat cgcgatttgc catgtcgcca agcagctcca ttcccgccgt
    29761 gccgccatcg aggatctcaa cataatccgg cagaatgtat cgttgctcta acgcttcgac
    29821 aatccgcacg ccgatggctt catcggtcag caaaatattg ccgaccccta agactaaaat
    29881 acgcattaca gaaccttcac tgaaaccact tcgttgccgt cggcatccac tacgtgtacc
    29941 gcacaggcca tgcacgggtc gaaggagtga atggtacgca ccacttccag cggtttattc
    30001 ggatcggcaa ccggtgtacc caccagcgac tgctcgtaag gaccgacgtc atcattgaag
    30061 ttacgcggac cagagttcca ggttgatgga acaaccgcct ggtagttgct gatgataccg
    30121 tctttaataa ccatccagtg agagagcata ccgcgcggcg cttcgaggaa gccaacacct
    30181 ttgaactcac ccgttgccgg aatgttcggc ttcacaaagg tggtgtgatc gcctttgccg
    30241 atattggtga tcagtgcact gtattggttt tgcaggatat cctgcaattc acagcagtga
    30301 acggtacgac caataatacg gcccagcgtg gagtgcagct gtgccacttc cagcgtgttg
    30361 ccagtcagtt tctgataaat cgcaacgatt tcattcagtt tgttctgggt agattcgcga
    30421 cctgccgcca gtttcaccag catattagcc agcggaccca cttctaccgt tttgccgtag
    30481 aaagtcggtg atttcaccca ggaatatttc ccgtcgtcag accaaccatc ataagccgga
    30541 atggtggtac cttcccacgg cgcctgtggc gcttcgtctt tataccagga gtgcttcgcg
    30601 ctttcctgaa tgcctttgat caggtattca tcggaatgag aagtgatcgg acgatacgag
    30661 gacagatccg cattctcaat gtagcctccc gggaacagga agctgccgtt tttgctgtcg
    30721 gtcgggaatt ccggcacgct caggtagttc accgcacctt taccgcgcgt cagccattcc
    30781 gggtagaacg cggcgataac cgcggtatca accttataaa cctgctcaac aaagtcgctc
    30841 agtttgtcga tgaaagactt gatgtacatc aggcgctcaa ggttcaacac gcccaacccg
    30901 tcgaggttga ttgggttcgc gacaccaccc acagccaggt tctgaatgtg cggcgtttta
    30961 ccgcccagca gcgccacgac gcggttagcg tcacgctggc actccagcgc ctgcaggtag
    31021 tgcgctaccg caatcaggtt cacttccggc ggcagtttca tcgccgggtg accccagtag
    31081 ccattggcga aaatacccaa ctgaccgctg gcaaccagat ctttgatctt gttctgaact
    31141 ttagtgaact cttccggact gttcagatgc caggtcgaaa cgcctttcag catttcggag
    31201 gctttggttg ggtcagcttg cagtgcagaa gtgatgtcca cccagtccag cgccgaaagc
    31261 tgatagaaat gaacgatatg gtcatgcgtg gtgtgcgcag ccagaatgat gttacggatg
    31321 tattgcgcgt taaccggaac gtcgatattc agcgcacttt ctgccgcacg aacggaagac
    31381 agcgcgtgag tggtagtaca tacgccacag atacgttgca caatcatcca tgcatcgcgc
    31441 ggatcgcggt ttttcacgat ctcttccatg ccgcgccaca tggtaccgga agcccatgct
    31501 ttcgaaacga cgccattttc gatttcgcaa tcgatgcgta aatgcccctc aatacgggtt
    31561 accggatcaa tagtaattct ctggctcatg ctttgctcgc ctcatgacga ttatgatcgt
    31621 tttgttttaa aggaggaagt atcggcagta gacgaatgag tacgatgtaa gcgcaaatct
    31681 caatagccac aaaaccaata gaaatcaaca gttcttccca ggtcgggaag taggcgtaac
    31741 cgccgcccgg gttgaatgcc accaacgaat aggtcagacg ccaggttgca caacctaaca
    31801 gtgcgctcag tgctgacagg aacagcatac gggaatcatt acgcagcttc gccacacgca
    31861 gaacgaccag cgggaagagc atcagcagga cttcaatcca gaacatcacg gagtagaagt
    31921 caccggcaaa cgctaacgac agcttgtcgc gatagataag ctcgccaaag cgcagcccga
    31981 tgaaaatcgc cagcaacaca ctgatggtgt tggtcagctt gacgaacagg ctcttttcat
    32041 ccggaccgtt gccacgcaga cccgcctgca ccagtgaacc ttcaaagatg acaatcgaga
    32101 agcccatgat gaacgccgtc agcagcgaga acagcggcaa catttcatag ctctgccaga
    32161 gcggatgcac cttgtagccc gccgagatca tcagcgaccc cattgaagac tggtgcatgg
    32221 tcggcagcag cgcaccgagc gcgatgatga agaacatcac cttgttcaga cgttgtagcg
    32281 acaccttcca gcccagacgt tcaaacagtg ccggagcaaa ctccagagcc atcacgccga
    32341 tgtagatggt catacagacc gccgtctcga acagtaccga gttcacgttg aagtgacccg
    32401 gaatgtagaa gtacggcagg ttccagtagc gacccacgtc gatagtgatc gacaagccac
    32461 ccagtgagta accaaacaga ctcgccaaca gcgccggacg caccagcgga tggtattgcc
    32521 cacggttaaa gacgtatacc gcccacgcca gcgcccagcc gccacaggca aagccggtgc
    32581 caatcagcag gtcaaacgcg atccacacgc cccacgggaa gccgccgttc aggtcagaga
    32641 cagagcccag accgaacacc agacgcttca caatcaggag catacagatg acgattaacg
    32701 gtccaaaaat catgaccggt ttactgatga ttttgccgcc cagcggttgt ggatcatgac
    32761 tcatgatcgt ctcctccgtc gtgatggtcg tttttggtgt tgcgacgaac cagcacggtt
    32821 aagcccgcca gcacagccag tggtagcatc atgcctttat acagggtgtg ttgaatattt
    32881 tcggaacgcg caccggtaga aagatcgtcc agtttcggca ggtcgagatt ttcataaggc
    32941 acacccgtca gtaccagaac ctgagtaccg ccgccctctt tctcgccgta cagatgcgga
    33001 taatatttcg gcaccgtatg caggtaagtg tcgccagatt tcagcgtctg acgcggatag
    33061 tggtattcgc tgccaggctt cagcgccaga cgttttttcg cctccgccat cagctcttca
    33121 cgcgtaccga aaatcaccgc gcccgccggg cacacttcta cgcagccagg cagaccgcct
    33181 ttatcgagac gttccacacc tttctggttg cacagctcgc acttatgcag cgcaccaaac
    33241 gggttgttgt agtcgtactt cggcacgttg tacggacagg cgaccatgca gtaacggcaa
    33301 ccggtgcaca cgtctttgtc gtaatggaca atgccggttt tcggatcttt tttcagtgca
    33361 gagaccgggc acacagagac acagttcgga tcgacgcagt gcatacactg tttcttaatg
    33421 tacgcgtagc cgttctcctc ctggtctttg ttgacccctg tgccgctggt ccacacctga
    33481 atgatgttat tggtatacgg cgacagtttg tcgttgttcg accaggtctg ttccccttgc
    33541 gggttacgtt cagggaagtt gatatcctga cacttggtga cgcaagcctg gcagcctacg
    33601 cacaaggtcg agtcgtacaa catccccagc gatcccggaa ttggcgggcg gttttcagca
    33661 gccgcatgac tgacagacgg cagcgcgccc gtcagcaatg ccccgcagga ggctgcttta
    33721 ataaaattac gtctgttcac ggttattctc cccgtgagtc agcgttatct ttcttttgct
    33781 gacgacccag ttcacgcacc gccatcacgc tgacaccggc aaccagccca accacaccgc
    33841 cgagcaaacc aatagcgcct gcagagacgt tgccgccctc tttagcgtta acatccggtt
    33901 tctgtgaacg cggagtctga ttttcgacgt tggcaagctg atggatgcct ttatggaagc
    33961 cgataccttt ttcgttacag ccatagcacg ggtgaccaat cgccaccggc cacacaccgc
    34021 caacatcgca gaattgcagc gttgagcagt tgccgtaagt ttctggccct ttacagccga
    34081 ggtggtacaa gcaccagcct tcgcggtggc cttcatcacc gaactctttg gcaaaacgac
    34141 cagcatcgaa gtgcgggcga cgttcgcagt gttcgtgaat cagacggcca taggcgaagg
    34201 tcggacggtt tttgtcatcc agtttcggcg gtttgccgta agtgatgatg tgcgcaacgg
    34261 tcgcgaggaa gttgtgcggg ttcggcgggc agcccggaat attgataacg gttttgcctg
    34321 gcagaacttc ttgcaagctg actgcgccag ttgggttaac tccagctgcg gcaacaccgc
    34381 cccacgcgga gcaggaacca atagcgataa tggctgctga gccttccgcc gctttgcgga
    34441 tgtgatccac aatcggctca ccggcaacca tgcaataaat accgttatct tttaatggga
    34501 tggaaccatc caccactaac acatactgcc ctttgtactt ctcgagagcg ttatgtttgt
    34561 tctcttcgac ctgatgaccg aaggcggcgg aaagcacttc gtgatattcc agagagatag
    34621 tctccagcac gaggttttct accgttggat gcgttgcacg aagcagagat tccgtacaac
    34681 cggtgcactc ctgcgcgcca atccagataa ctggcggacg ctgcgggtta gtaaccgatt
    34741 cggccatctc tgcagcggct ttgctactta accccatggt ggcggctaat gctgcacaaa
    34801 gcttcatgaa atcacgacgg ttaatgccgt gagaatggat gagggtgtta tctccagtca
    34861 tttatagtta ttccgttgcg agaacctggc atatattttg cctcaatcgc aaaatcaata
    34921 atgcgatcga tgcgccattt accacacatt tattatggtt atcgatatca tgatactgcg
    34981 gcggggcgct aaaacgaagg gaagaagcat tagtgtaatt aattaaagca agataatacg
    35041 tatgtttgat caattttcga ggcgataacc ggccataaaa aagcgcaacg tagaacagga
    35101 attattctca cgaaagaaat ccattaccat ctctttatcc agtccatagc ggcgcgtcag
    35161 aatacccgct tcccaggcgc taagctggcg atctccggtc ttttcgtaca tcctgtgtga
    35221 gtagcctaat acaaaaccgc gtttataatc ggcacagaat tttgtcacat taaccgcact
    35281 gtcggcatgc gttgctttta atcccgccat taagccttta ccaaaatggt tattcatcgt
    35341 tcacctcatt cattatatat cgccatatat aacgatattt taggcaaaga acgacttttg
    35401 cgcacgctat tttttcatac ttgtttaaga cgtaatcccg taagagataa acctatcagg
    35461 agagatattt atcgaaccat ttcagtgtcc tttgccaggc aagatcggcg gcagatttgt
    35521 cataacgggg cgtggaatca ttatggaatc cgtgattaac ccccggatag atataagcct
    35581 cataaacctt attattgtct ttcaacgccg cctcgtaagg aggccagccc tcgttgattc
    35641 gggtgtccag ttccgcgaag tggagtagta aaggcgcttc aatcttcgcc acatcggcag
    35701 tgggtgcctg acgaccataa aacggcaccg cgcaggccag ttccggatac gcgacagccg
    35761 ccgcgttcga tacgccaccg ccatagcaaa atccggtaat acccacttta cctgtcgctt
    35821 gcggatagcg ttgcataaac tcaattgcgg caaagaaatc attcatcagt ttggttggat
    35881 caacctgctg ttgcagctca cgacctttat catcatttcc cggataacct ccaacggaac
    35941 ttaatccgtc aggtgccagg gcgatatacc ccgccttcgc cactcgccgt gccacatctt
    36001 cgatatacgg attcagtcca cgattctcat gcaccacccc cacggctggc gttttgccgc
    36061 tcatttttgc gggtttcacc agataacccc gtacctcgcc gtgaccattt ggcgaaggat
    36121 acgtgatgta ctcagcaaca atctccgggt cggtaaactc tacctgagtc gccagcgcat
    36181 aatttggctt gagcaaatca aacaacgcta atgccgtcat cccgcccacc gcatacttcg
    36241 ccgcaaggtt gaggaactca cgtttcgaaa ttttcccgtg aacgtaatag tcgtagtaat
    36301 ccaacaactc ttgtgggaaa tctttggcgg ttaaacgcgg catcgttgca ttcctcaact
    36361 ggtgtttttt taagcaaagc ataagcgcgt gtttttgccc agtttttcgt cactctgtga
    36421 gccagactac gggatacgcg ctggcgaatc gctaaactag aaacattgtt tcgaaattga
    36481 acggtggaaa ggagaggtca tggtctggtt agcgaatccc gaacgttacg ggcagatgca
    36541 gtaccgctat tgcggaaaaa gtggtttacg cctgcccgca ttatcgctcg gtttatggca
    36601 caatttcggt cacgttaacg cgctggaatc acagcgtgca atcctgcgta aagcgtttga
    36661 tttaggcatt acgcactttg atttagccaa caattacggg ccgcctccag gaagcgcaga
    36721 agagaacttt ggtcgcctgc tgcgcgagga ttttgctgct tatcgcgatg aactgattat
    36781 ctctaccaag gctggctacg atatgtggcc cggcccgtac ggctctggcg gttcacgtaa
    36841 atacctgctc gccagcctcg accaaagcct gaagcgtatg gggcttgagt atgtcgatat
    36901 cttttactct catcgcgtcg atgaaaatac gccgatggaa gaaaccgcct ctgcgctggc
    36961 tcatgcggta caaagcggta aggcgctgta tgtcgggatc tcctcttact cgccagagcg
    37021 gacgcaaaaa atggtcgagt tgctgcacga gtggaaaatt ccgcttttaa ttcatcaacc
    37081 ttcgtacaat ttactgaacc gctgggtgga taaaagcggc ctgctggata ccctgcaaaa
    37141 taacggcgtg ggctgtattg cctttactcc tctggctcag ggattgctga ccggaaaata
    37201 tctcaacggc attccgcaag attcacggat gcatcgtgaa gggaataaag ttcgtggtct
    37261 gacgccgaaa atgcttaccg aagccaacct caacagcctg cgcttattga atgaaatggc
    37321 acagcagcgt ggacaatcaa tggcgcaaat ggcgttaagc tggttgctga aagatgatcg
    37381 cgtgacgtcg gtattgattg gtgccagccg cgcggagcaa ctagaggaga acgtgcaggc
    37441 gctgaataat ctgacattta gcaccgagga gctggcgcag attgatcagc atatcgccga
    37501 tggcgagctg aatctgtggc aggcgtcgtc cgataaatga cctgttaata acgggcgcgc
    37561 gaaccgcgcc cgaatcagat cagtgattat gacgagtcag tcggtcaaga tagcccatca
    37621 caaatgcaga aagcacaaac gtcagatgga taatgacgta ccacatcagt ttgttatccg
    37681 ggacattttt cgcatccata aagacgcgca gtaagtgaat ggaagaaatt gccacaatcg
    37741 acgctgctac tttgtttttc agcgacgttg cgtccatttt ccccagccac ttcagcttct
    37801 ctttgttctc ggagatatcc agctgtgaga cgaaattctc ataaccggaa aacatcacca
    37861 tcaccagcaa accgccaacc agcgtcatat ccaccagcga cagcaacacg aggatcaaat
    37921 ctgattccgc catcgagaag atattcggca gtacgtgaat aatctcctgg aagaacttca
    37981 gcgccagggc aactaacgcc agcgaaaggc caaagtacac gggggcaagc agccagcgag
    38041 aagcatacat tgcattttca agaaaacgtt ccatacattc ctgtcgtgta gttaaaacgg
    38101 gcaacagtat atcgcaattg agtaaacagc acgcaacagc aaatcaacat atacgttaaa
    38161 cctttgtcgc ataattctga ggtttaggga tcttcctggt tcccttatca acgcatcgag
    38221 agcgtctacc cttaaaggac gttgaacctg aagggagaaa aacgatgtct catttaaaag
    38281 acccgaccac gcagtattac actggtgaat atcccaaaca gaaacaaccg acgccaggca
    38341 tccaggcgaa gatgacaccg ataccggatt gcggcgagaa aacctatgtt ggtagcggtc
    38401 gcctgaaaga tcgtaaagca ctggtgacag ggggcgattc cggaataggt cgcgctgccg
    38461 ccatcgctta cgcgcgtgaa ggggctgacg tggcgatcag ttatcttccc gtggaagaag
    38521 aagacgctca ggatgtgaaa aagatcattg aagaatgcgg acgcaaagcc gttctgctgc
    38581 caggcgattt aagcgatgag aaattcgccc gttcgctggt tcacgaagcg cacaaggcgt
    38641 taggcgggct ggatattatg gcgctggtcg ccgggaaaca ggttgccatt ccggatattg
    38701 cagacctcac cagcgaacag tttcaaaaga cctttgccat taacgttttc gcgctgttct
    38761 ggctaaccca ggaagcgatc cccctgctac cgaaaggtgc aagtgtcatc accacttcgt
    38821 caatccaggc ataccagcca agtccgcatt tactggacta tgcggctacg aaggcggcga
    38881 ttctgaacta cagccgtggc ctggcaaaac aggtcgcgga gaaaggtatt cgggtgaata
    38941 ttgtcgcgcc aggccctatc tggacggcac tgcaaatttc cggcggacaa acgcaggata
    39001 agatcccgca gtttggtcag aaaacgccga tgaagcgtgc ggggcaaccg gcggaactgg
    39061 cccctgtata tgtttatctg gcaagtcagg agtcgagcta cgtcaccgca gaagtgcacg
    39121 gcgtgtgcgg cggcgagcat ttaggttaaa aaaatgcccg gttgtgaaaa gcaaccgggc
    39181 atcattgtga attcactctt acctgatgtg gcgaaaacgc cttatccgcc tacaaacgca
    39241 tgaagattca acatgttgca atagattttg taggcctgat aagcgtagcg catcaggcat
    39301 tgagcaccta atgccggatg cgtcgtgaac gccttatccg gcctacaaac gcacgaagat
    39361 tcaacatgtt gcaatagatt ttgtaggcct gatgagcgta gcgcatcagg caattctact
    39421 ttacttcgct ttggcggttt cttcgccgac cagacctatc ttcaggtaac ccgcctgatg
    39481 cagcgtatcc attaccttca tcaacgtctc gtaatcgacg gttttgtccg ctcggaagaa
    39541 gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca ttgtttcatc
    39601 ggtgaccgga tcgttaccga taaacatcga gttgtctgcc ttcaccgaca gataaaccgg
    39661 tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aagttcacct tcacatctac
    39721 cgtcgctaac ggtgccgcca ccataaagat aatcagcaga accaacatca cgtcgataaa
    39781 cggcgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt taagatgcat
    39841 tgccatcgga cattatcctg cgcgtaattt ttgtgcgaca cgaaccggat gcgcagcggc
    39901 gctggcttcc agatctaggt cacggctttg cagcaacaat acctgcgctg caacatcacc
    39961 cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa cgaccgcagg
    40021 aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcgatac ccggcgcaac
    40081 gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca tgatgcccca
    40141 gaccgtacca aacagaccaa cgaacggaga aatcgcgccg atggttgcca gatagccgtt
    40201 accgcggccc atttgacgac ccactgcggc gacccgacgc tccagacgga agctggtacg
    40261 ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat tctgcgcttc
    40321 attgagcaaa tgcaggctta agcttttgct accaaaatca gcggcgatat cgttggcctg
    40381 gtttaaggaa cgtgcttcag ccagcagttg ctgctcgcgc ttaaggcgac gcttctgatt
    40441 gaagaactct acgctcttac tgaagaagat tgcccaggtg actacagagg ccaaaataag
    40501 cccaatcatc acgcacttaa cgacaatatc ggcgtactga tacatacccc agacggaaag
    40561 gtccgtctgc attaaattat tacccactgt gtatctccag gacgcaagtc acaaaatctg
    40621 cgcataataa tatcaaaacg acgtcgaatt gatagtcgtt ctcattacta tttgcatact
    40681 gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtcacc agcacgccat
    40741 ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat attcatgcta
    40801 gtttagacat ccagacgtat aaaaacagga atcccgacat ggcggacaaa aagcttgata
    40861 ctcaactggt gaatgcagga cgcagcaaaa aatacactct tggcgcggta aatagcgtga
    40921 ttcagcgcgc ttcttcgctg gtatttgaga gtatggaagc caaaaagcac gcgacgcgca
    40981 atcgcgccaa tggcgagttg ttctatggac ggcgcggaac gttaacccat ttttccttac
    41041 aacaagcgat gtgtgaactg gaaggtggcg caggctgcgc gctatttccc tgcggggcgg
    41101 cagcggttgc taattcgatt cttgcttttg tcgaacaggg cgatcatgtg ttgatgacca
    41161 acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa ctgggcgtaa
    41221 cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg cagccaaaca
    41281 ctaaaatcgt gtttctggaa tcgccgggtt ccatcactat ggaagtccac gacgttccgg
    41341 cgattgttgc cgccgtgcgc agtgtggtgc cggatgccat cattatgatc gacaacacct
    41401 gggcagccgg tgtgctgttt aaggcgctgg attttggcat cgatgtttct attcaggccg
    41461 ccaccaaata tctggttggg cattcagatg cgatgattgg cactgccgtg tgcaatgccc
    41521 gttgctggga gcagctacgg gaaaatgcct atctgatggg ccagatggtc gatgccgata
    41581 ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgt caacatcatg
    41641 aaagcagtct gaaagtggcc gaatggctgg cagaacatcc gcaagttgcg cgagttaacc
    41701 accctgccct gcctggcagt aaaggacacg aattctggaa acgagacttt acaggcagca
    41761 gcgggctatt ttcctttgtg cttaagaaaa aactcaatga tgaagagctg gcgaactatc
    41821 tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa tcgttgatcc
    41881 tggcaaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc gattttagcg
    41941 ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt gccgatctgg
    42001 acgccggttt tgcacggatt gtataacatt gccacttttg gacaattttg cagacatttt
    42061 attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg attcaggagt
    42121 acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc tgttattcaa
    42181 gatatcatcg ctgcgctctg gcaacacgac tttgccgcgc tggcggatcc tcatattgtt
    42241 agcgttgttt actttgtcat gtttgccacg ctgtttttag aaaacggcct gctgcccgcc
    42301 tcatttttgc caggcgacag cttgttgata ctggcaggcg cattgattgc ccaaggggtt
    42361 atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg ctgctggcta
    42421 agttatattc aggggagctg gttagggaat accaaaacgg tgaaaggctg gctggcacag
    42481 cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg tctgctggcg
    42541 ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat ggcgggaatt
    42601 tccggtctgc caaaccgccg cttccagttt ttcaactggt taagtggatt gctgtgggtc
    42661 agcgtggtaa ccagttttgg ctatgcctta agtatgattc cgttcgttaa acgccatgaa
    42721 gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc tggcttgtta
    42781 ggcacgctgt ttgtggtgat taaaaaaaaa tactgtaacg cctgacgatt ttccccgttc
    42841 ccggctgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc ttgccgcatc
    42901 ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgcg atgcgctttc
    42961 atagccgacg cgcatcgctg ctgcgctggc cttcatgccg tcatggatga tcatcatccg
    43021 cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg tgacagactt
    43081 aaaattatgg tggaacgccg atacgctcat gttggcttct gccgccagtt gctcgacgct
    43141 caggttttcg gtgtatttat tctcaattcg tttcagcacg cggctaatca gactgaagtg
    43201 agtctggcga ctgaccagcg ccagtaacgc gccgccgcaa ggtccggtca gcacgtagta
    43261 cagaatttcg cggatgatct gtttgccgag aatacgcgca tccagtggtc gctccatcac
    43321 gtcgagtaac cgctccgccg cgcataaaat ctcttctgat aacgtggcgg agttaatccc
    43381 gctggctgcc atcgacggct ggaaatgctc atcttcgcca atgtccatca acagttcctg
    43441 taactgcaaa atatcgacat tgagacgcaa ccctgccagc ggcacctctg acgtggcata
    43501 ggtttcgcac tcaaacggca acggcaccgt cagcagcagg tattcattgg catcataacg
    43561 aaacacgcgt tcattgatat aaccgatttt atgcccggaa aagagaatta tgatgccagg
    43621 ctcgtacatc accggtgtac gtgcgaaagg cgtctcgcca tacaacaaac gcacatcggg
    43681 caacagttct gacaaactat tttctttatt tttcagttta ttaactttat ccgccagcaa
    43741 gcgacaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc ggcaatttga
    43801 ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag acattggcag
    43861 aaatgagcat tgagagccag ggcgctggcg atcacaatga aaaacatcag gcagatcgtt
    43921 ctctgccctc atattggccc agcaaaggga gcaagtaatg aacaacttta atctgcacac
    43981 cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac aaattcctca
    44041 cgatgctcgc gtattgatta cctacggtgg cggcagcgtg aaaaaaaccg gcgttctcga
    44101 tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta ttgagccaaa
    44161 cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaga aagtgacttt
    44221 cctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg ccgcagcggc
    44281 taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggta aagagattaa
    44341 aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag aatccaacgc
    44401 aggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt ctgcacatgt
    44461 tcagccggta tttgccgtgc tcgatccggt ttatacctac accctgccgc cgcgtcaggt
    44521 ggctaacggc gtagtggacg cctttgtaca caccgtggaa cagtatgtta ccaaaccggt
    44581 tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctaa tcgaagatgg
    44641 tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca tgtgggcggc
    44701 gactcaggcg ctgaacggtt tgattggcac tggcgtaccg caggactggg caacgcatat
    44761 gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac tggctatcgt
    44821 cctgcctgca ctgtggaatg aaaaacgcga gaccaagcgc gctaagctgc tgcaatatgc
    44881 tgaacgcgtc tggaacatca ctgaaggttc cgacgatgag cgtattgacg ccgcgattgc
    44941 cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg actacggtct
    45001 ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca tgacccaact
    45061 gggcgaaaat catgacatta cgttggatgt cagccgccgt atatacgaag ccgcccgcta
    45121 agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa gttcacaaca
    45181 cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc taatccaacc
    45241 gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt ctggcaagca
    45301 agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta tcgctcgatt
    45361 gataccgccg cggcctacaa gaacgaagaa ggtgtcggca aagccctgaa aaatgcctca
    45421 gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca caagcgcccc
    45481 cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga cctctactta
    45541 atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg catgatcgaa
    45601 ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat ccatcacctg
    45661 caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga acttcatccg
    45721 ctgatgcaac aacgccagct acacgcttgg aacgcgacac acaaaatcca gaccgaatcc
    45781 tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat tcgcgatctg
    45841 gcagataaat acggcaaaac cccggcgcag attgttatcc gctggcatct ggatagcggc
    45901 ctggtggtga tcccgaaatc ggtcacacct tcacgtattg ccgaaaactt tgatgtctgg
    45961 gatttccgtc tcgacaaaga cgaactcggc aaaattgcaa aactcgatca gggcaagcgt
    46021 ctcggtcccg atcctgacca gttcggcggc taacgtgcaa attctcccgg tggcggtaat
    46081 gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc cgttgttact
    46141 cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg aagctcttaa
    46201 aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac agatgaaaat
    46261 aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcgcagc ctccttcaca
    46321 gaccgtagaa caaacagtcc ggcagattta tcagaactat aaatcagatg ccagtacgcc
    46381 ttatttcggt gaaaccggag agcgggcgat aacttcagcg cgtattcaac aggcgcttac
    46441 cctgaacgac aatcttacac taccgggcaa tattggctgg ctggattatg atccggtttg
    46501 tgattgtcag gattttggcg atctggtgtt agaaagcgta gcgataaccc aaactgacgt
    46561 cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gatgataaag aaaagaccac
    46621 acagacactg aaaatggtgg cggaaaatgg tcgttgggtc attgatgata ttgtcagcaa
    46681 tcatggcagc gtcttacaag cagttaatag cgagaatgaa aaaacgctgg ccgctttagc
    46741 ttcgttgcag aaagaacagc cagaatcctt tgttgctgaa ctctttgaac atattgctga
    46801 ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg ttaatgcctt
    46861 ctataaaacc accttcaaga cggccaataa tcccgatgaa gatatgcaaa tagaacggca
    46921 atttatttac gacaatccga tctgttttgg cgaagagtcg ctattttcac gcgttgatga
    46981 aattcgagtc ctggagaaaa ccgccgattc cgcccgcatt catgttcgtt ttacgctgac
    47041 caatggcaac aatgaagagc aagagctggt tttacagcgg cgcgaaggta agtgggaaat
    47101 cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg caaaaaccgc
    47161 cgccagatta aagcaatgag ctgaattaaa taacaattag ccggaacaat aaataaaagg
    47221 gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac ctgcttgctc
    47281 agtggctgcg tcaatgagca aaaggtcaat caactggcga gcaatgtaca aacattaaat
    47341 gccaaaatcg cccggcttga gcaggatatg aaagcactac gcccacaaat ctatgctgcc
    47401 aaatccgaag ctaacagagc caatacgcgt cttgatgcac aggactattt tgattgcctg
    47461 cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcatgt cagttgccgg
    47521 ggattttttt taacgtccaa ccgccgcttt agggcgtttc ttcgcaccag cattcaccgg
    47581 acgagattgc gtagacgacg ctttttttgc cgtagcaggc gtctgacgct gagtcgccat
    47641 cggcgtatgt ttagtcaacg ccggacgggt attgcggttc tggcgacgag cttcacgcat
    47701 ctcttcaatg gttggcgcag gcactaagca atcgcgacgg ctgccaatca gatgcttttt
    47761 gcccatcgct tccagcgcct ggcggattaa cggccagttt gccggatcgt ggtaacgcaa
    47821 caacgcttta tgcaaacgac gctgtttgtc gcccttcggt acgaagacgc cttcgctctt
    47881 ataaccaatc ttcgccagcg gttttttccc ggtgtaatac atggtggttg agttcgccag
    47941 cggcgatggg tagaagttct gtacctggtc gagacggaaa cgatgctttt tcagccacag
    48001 cgccaaattc accatatctt catcacgcgt accggggtgc gcagagatga aatacgggat
    48061 cagatactgc tctttacctg cctgtttcga gtaggtatcg aacagctctt taaagcggtc
    48121 atagctgccc atgcccggct tcatcatctt cgacaacggc ccttcttcgg tatgttccgg
    48181 agcaatcttc agataaccgc cgacgtgatg ggtcgccaac tctttgatat agcgcggatc
    48241 ttccacggcg atgtcataac gtacgcctga ggcgataagg atttttttaa tgcctttcag
    48301 atcacgcgca cggcgataga ggttgatcgt cggttcgtgg ttagtgtcca tgtgcggaca
    48361 aatatccgga taaacgcacg acaaacggcg acaagtttgt tcagcgcgtg gcgatttgca
    48421 gcgcaacata tacatgttgg cagttggccc accgagatcg gaaatcacgc ccgtaaaacc
    48481 tggaacggtg tcgcggatcg cttcgatctc attaatgatc gaatcttccg aacggctctg
    48541 aataatgcgc ccttcgtgct cggtaataga acagaaagaa cagccgccaa agcagccgcg
    48601 cataatgttg accgaaaaac ggatcatttc gtaagccgga atacgggcat tgccataggc
    48661 cggatgtggc acgcgcttgt acggcagcgc aaaaacgctg tccatctctt cggtagaaag
    48721 cgggatagca ggcgggttga tccacacata gcggtcaccg tgtttttgca tcaatgcgcg
    48781 ggcgcagcct gggttggttt cgtggtgcag aatgcgcgaa gcatgggcgt acagcacttt
    48841 atcgcccttc actttctcga aagaaggcag caatacgtag gttttttccc acggtttctg
    48901 gcgcggtggc tgcacgatta cagctttcgc ttcctgcttt ttcggcgcaa caggtttgtt
    48961 atccgcacac ggcaaatctt cgccatacgg atgcgggatt gggtcgattt tccctagggt
    49021 atcaagacgg gtggaatcca caccgctcca gccaggcagc gcctctttta cgataattgc
    49081 ggtattacgc acatcgcgga tttcactaat cggctcgccc atcgccagac gatgcgctac
    49141 ttccaccagc ggacgttcac cgttaccaaa catcagcatg tcggctttcg aatctaccag
    49201 aacggaacgg cgcacggtat cggaccagta atcataatgc gcggtacggc gcaggctagc
    49261 ctcaataccg ccgaggatca ccggcacatc tttccacgcc tctttacaac gctgggtata
    49321 aaccagcgtg gcgcgatccg gtcgcttacc cgcgacatta tctggcgtgt aggcatcatc
    49381 atgacgtaaa cggcgatcgg cggtatagcg gttgatcatc gaatccatgt tgccagcagt
    49441 aacaccaaaa aacagattcg gtttacccag acgcataaag tcgtctttgc tgctccagtc
    49501 cggctgggcg atgatcccga cgcgaaagcc ctgcgcttcc agcatacgac cgcaaatcgc
    49561 catcccgaag cttgggtgat cgacatacgc gtcgccagta accaaaatga tgtcgcagct
    49621 atcccagcca agttgatcca tctcttcacg agacatcggc aaaaacggtg ccggtccaaa
    49681 acaggcggcc cagtactgcg gccaggagaa caggtcgcga tccggttgga tcagggagat
    49741 agagctcatt ttgcttccag aaatgataaa aaaataatca aaggccgggg attataagcc
    49801 ggaacgaaag agaaatcgaa aggtattcca tactcgccct cctcgggcga gtatgaagat
    49861 tacggtaccg gattgaccaa cagttgccca atcgagccac ggtccgccat ttccagcgtc
    49921 tgactgttga agtagaacgg gaagtgcgcc caggaaggct gaccgaaata aacaagcagc
    49981 tccacttgtc cgtcaaccca aacggtatct ttccagcctc tgtcttccgg gaacggcatc
    50041 gcgccattca cgttacggat ctggaacatt acgccttcaa tatggaacgc ttgcggctcg
    50101 tccgcgcgta ccgtccagcg ttcccacgtt ccttgctgcg cggtgacatc aatacggttg
    50161 acgtcccaca gctgtccatt aatacccggg tcatcaccca gactgatatc gcgactgcga
    50221 attggcgaac cagccatgat ttcagttggc agcaagcgca tcggaagact gtctgtgacc
    50281 agcggcagaa ggccggttgg gcgcagcgtt agcaccaagg tagaaaccag aatactggat
    50341 ggctcaaaga agccgcgaat acgatcaaca atgctcgccg cttcaccaca ggtgatcgac
    50401 acttcatcgc cgttgctcat atccaccaga atctcgcggc gctcgcccgg tgccagcgaa
    50461 agttgcttca ccgatacagg agcaggcagg aatccctgat cgccagaaat cacatgtaac
    50521 gggcgaccat cgctcatctg tagttgatag cgacgagagt tcgacgcgtt cagcagtcgc
    50581 aagcgcaccc agccacgcga gacttcaacg tacgggcttt gtacaccgtt aaccagcagc
    50641 gtatcaccaa caaagccgcc gcttcccggt tcgttgtatt ctggcgtacc aaagttatcc
    50701 agccgtttat cctggataat gaccggaaaa tcatccacac cataatggtt ggggataggc
    50761 agcgacttgc tgacttcatc ttccaccagc cacattccgg caaggccgtt atagacctgc
    50821 tgagccgtgc ggttgggagt attggcgtga taccacagag tagctgcgtt ctggcgaata
    50881 ggcagtacgg gtgcccagtc agcgtttggc gacatcatcc gtgccggacc gcccatcagc
    50941 gggcctggta cctgtagccc ggccaccgtc attgagacat tttctgtcag gcggttgctg
    51001 taaataagct taacatcgtc gcccttccag acgcggatag tcggccccag gtaacgacca
    51061 ttgattcccc agaccgacgc gcgtgtccct ggcgtaaatg accagtgcgc acgttgtaca
    51121 gtcataaaca gcggttgccc acggcgagat tcaagtagcg gcggaacggg tagcggttgc
    51181 tgttgcccgg ctgcgctggc cttcagggga acagcgcctg cacaaagtgc aatccccgat
    51241 gcctgaatga actgacgccg actgagtgac atatttgctc catgtaaaac tggctaaagt
    51301 aataccgtgc aggaaagtgt tccccgcaaa aatacagatt gccgacttaa acttttccgg
    51361 cggcttcgcg ttctgcgact tctttatcga gctcggcgat tttttgttcc attatcgaac
    51421 gacaatgggc agccagctca cgaacctgat ctttgccata ctgactgacg tcaattggcg
    51481 gcagcatttc gacaatcacc agaccgttgt gcagtcgatt aagattaatc ttattcgaag
    51541 ttgtagagac gcacacggga ataatcggga cgcccgccgc aattgccgcg tgaaatgctc
    51601 cagtcttgaa cggtagcagg ccgcgaccac ggctgcgggt tccttccggg aacatccaga
    51661 tggaaatacg gcgttttttg aaatgattca cgacttccgc aatggtgccg tgagctttag
    51721 tgcgattgtt tctgtcgatc aataagttgc cggttaacca gtacaactgc ccgaagaagg
    51781 ggatccacag caagctcttt ttacctaccg tcaccgtcgg cggttgcaca atgttcgatg
    51841 ctgtcaccat gtcatagttg ttctggtggt tagcgatata gatagcattg ccgtagcttt
    51901 cagcatctgc aggtttgcgg cactcaactt tcaggccgaa cagtggcgca agacggccaa
    51961 acatatgccc aaaggtggcc acatgtttcg ggttacgcgg gctgaaaagg cagtaaatgg
    52021 agccgaatac acagactaag atgctgtaaa tcacggtaat aataagacga aagatatata
    52081 gcataacacc ctctgaaggt ctgagagact ggcattgtac gtcacctgaa atcaggttag
    52141 ggcattgtta aaagccgctc tctggaaaag cgtattgtta atcgcaacgc atgaataaac
    52201 aacggtttca agggaatttt tatcgtttcc gggggttgaa tacgctgccg gatgacgact
    52261 taacgtttca tccggcgttc cttgctagcg ggaggaaaca gcgccctccc cggcatatta
    52321 ctcttcgcta tcaccgctgc tggcacggcg aggagagtcg atctcaacac gatcgatacg
    52381 ctgcaaaccg cgcatcaacg taccgcggcg tccacgttcg ccagtgactt tctgtagctc
    52441 ttccggacgc agtttaattt tgcgtttccc aacatgaatg gtcagcgtgc tttgcggcgg
    52501 cagaacgtac agttgcgcca gaccatcctc gccacgcgcg gcttctgccg atggaatgtt
    52561 gataatcttg ttgcctttgc ccttcgacag ctgcggcaga tcgcttaccg ggaacatcaa
    52621 catacggcct gcctgagtga ttgccagcag catatcggaa gcatcttcaa tcaccaccgg
    52681 cggcataaca tgggcatttt ccggtaaggt gatcaaagcc ttacccgcac ggttacgcgc
    52741 taccagatcg ttaaaggtgc agacgaaacc gtaacccgca tcggaagcca tcagcagttt
    52801 ctgatcgtcg ctttccatca gcatatggtc aacggtcgcc ccaggcggca acgttaattt
    52861 gccggtgagt ggttcgccct gaccacgcgc cgacggcagc gtaatcgggt cgatggcata
    52921 gctacgaccg gtggaatcaa caaacactac cggttggtta ctcttacctt tcaccgccgc
    52981 tttgaagcta tcacccgctt tataattcag gcccggcgcg tcgatatcat ggcctttagc
    53041 gctgcgtacc cagcccatct gcgacagcac aatggtgaca ggttcagacg gcagcatgtc
    53101 gtgctcgctc atcgctttcg cttcttcgcg ttcctgcaac ggcgaacgac gatcgtcacc
    53161 gtaggcttgc gcgtctgcct gcagttcttt cttcagcagg ttattcattt tacgctcgga
    53221 agccaaaatg ccctgcaact ggtcgcgctc tttttccagc tcactctgct caccgcgaat
    53281 cttcatctct tccagtttgg caagatgacg cagtttcagt tcgaggatcg cttccgcctg
    53341 ggtttccgta aggccaaacc gcgacatcag cgccggtttc ggttcatctt cgttacgaat
    53401 gatctcaatc acttcgtcga tgttgagaaa cgccaccagc aaaccttcca ggatatgcag
    53461 gcgcttgagg actttctcca gacgatagtt cagtcggcgg cgcacggtat cgcgacggaa
    53521 caccagccat tcggagagga tttccagcag gtttttcacc gccggacgac catccagacc
    53581 gatcatattg aggttaatgc gatagctctt ttccagatcg gtggtagcga agaggtggtt
    53641 catcacctga tccatatcca cgcggttgga acgcggcaca atcaccagac gggtcgggtt
    53701 ctcgtggtca gattcatcgc gcagatcgtc aaccatcggc agctttttgt tgcgcatttg
    53761 cgcagcaatt tgctccagta cgcgcgcacc tgaaacctga tgcggtaatg cgctgataac
    53821 caccgcgcca tcttctttct tccacaccgc gcgcatacgc actgaaccac gtccgttctc
    53881 gtagatttta cgaatctcgg cgcgcgaagt gataatttcc gcttcagtcg gataatccgg
    53941 cccctgcacg atatccagca gctgatcgag cgtagttttc ggctggtcga ttaatgcgat
    54001 tgccgcctga gctacttcac gcaggttatg cggtggaata tcggtcgcca tgccgacggc
    54061 aataccggtg gtgccgttaa gcaaaatgtt tggcagacgg gcaggtagca ttttcggctc
    54121 ctgcaaagtg ccgtcgaagt ttggcaccca gtcagccgtc ccctgcccca gctcgctcaa
    54181 tagcagctcg gaatatttcg acaaccggga ttcggtgtaa cgcattgccg cgaacgattt
    54241 cggatcgtcc ggcgcgcccc agttcccctg accatcaacc agcggataac ggtaagagaa
    54301 cggctgcgcc atcaggacca tcgcttcata acaggcgcta tcgccgtgcg gatggtattt
    54361 acccagtacg tcaccgacgg tacgggccga ttttttaaat ttggcgctgg cattcaggcc
    54421 cagttcagac atcgcataca caatgcggcg ctgaacaggt ttcagaccat caccaataaa
    54481 cggcaacgca cggtccatga tcacgtacat ggagtagttt aagtaggcgt tttccgtaaa
    54541 ttcatgtagc gcaaggcgct ctgccatatc gctcattaat tctgattcct caacttattc
    54601 gccagccttc aaaggggcaa tattgccgga gatactacat tatctgccgc gtcgagtcac
    54661 aggagatcag cgtgaatagc gccgccggac gtgctccgac ggcgataaag gcatcaatgt
    54721 gccttgatat acaactcttt gctgtagact tgatcggcat agagggcagc acaaggttgc
    54781 gcccatgcca caccactcgg catgggggtc cgctccgggc gattcgagaa gttgaggtta
    54841 tgcgagtcca gggaagccta aagcatcaaa gtagtcgact gtaagtatac ggttcagccc
    54901 agtttagctg tgtctccacc agtgccccgc gcctcctgcc ataagtgcgg cttgctcatg
    54961 gatatccccg agacggctta cggcgcaccg gataataaat ttcgcgtata catggtattc
    55021 cttaccggag aaattataag cccaacaaca atacgcctgt gacattgttc gctcaaactt
    55081 atcgcgagtg atttggctca cactccggta aagattcccc atcacgccaa cggcacataa
    55141 acatccgttt ccagtcgctg ctctgtcacc ccttcggcaa gattggtgta gtgaaacaga
    55201 atcggtgctt tacgcatttt ctcgccgctt gcaggcagcc agtggcgaat aatgccccat
    55261 atcgtgtgtg aaatatcgtc cagctcgcca acgtggcggg ccacggcata acgtccaccg
    55321 gtaagttcac cattgctcac accataacga ttatcgggaa tcggttcgct aacgctgccg
    55381 cagatatcaa agcgaaacgc ttccggtgcg gtggtggcag gggcgtccca ggcgacgcca
    55441 aaagtctggc tttggttaac aggagaaagc cccgtctctt tacgccacat gataaatttc
    55501 gccacactgt agttgagcaa ctcagggcta cagcggtgcg gcaacatcgc cacagggatt
    55561 gatgggaaat cgataatgtt tacatccagt gtcaggtttg tcattttgcc tccctgcgaa
    55621 attattcccg ggaacgcgtc ccgggagcgg taacaattac ggatttactt tgcggatctg
    55681 tttgacgtca atttcaacag aattccagtc tttatcgact tcaccctgaa tctcaaccgt
    55741 atctttcggc gtcaccgtca caccgttcca gcgtttgtga tcgatatcaa cattgatagt
    55801 accgctggca tctttaaaca cgtagagatc gtcagagatg cgttcaacga tattgccgcg
    55861 cagggttacc caggtgtcgt cacgcaggga ttttgcgctt tctacagtcg ttacgctgcc
    55921 gttcggcccc tgaaatcctc cggcctgact ttgcgttacc gatgggccag aaaaaccgcc
    55981 ctgctctgct gccatcaccg gtgcactgca cagggccatt actgcgatta ctgctgcgaa
    56041 ttttttcatg tttattactc cctttaatgt ctgtttccga gcatttaaca agatagtcct
    56101 taacaacttc ttaagggaaa aaaataaaat ttaatgctgt acagagcgcg ttacaacacg
    56161 gtttactggc agcaaatacg gttatcgcag ggatgaaaaa atgcgaattt tactgataga
    56221 agatgacatg ctgattggtg acggcatcaa aactggcctt agtaaaatgg gttttagtgt
    56281 cgactggttt acacaaggtc gtcagggaaa agaggcgctt tatagcgcac cttatgatgc
    56341 ggtgatcctg gatttaacct taccaggcat ggatggtcgc gatattttgc gcgaatggcg
    56401 agagaaaggt cagcgtgagc cggtactgaa cctgaccgcg cgtgatgcgt tagcggaacg
    56461 tgtagaaggg ctgcgtctgg gagctgacga ttatctgtgt aaaccttttg cgttgataga
    56521 agtcgccgcc aggctggagg ctctgatgcg ccgaaccaac ggccaggcca gcaacgagct
    56581 gcgccacggt aacgtcatgc tcgaccccgg caaacgtatc gccacgctgg ctggcgaacc
    56641 cttaacactg aaaccaaaag aatttgccct gctggaatta ctgatgcgta acgctggtcg
    56701 ggtactgccg cgcaaactga ttgaagagaa actgtatacc tgggacgaag aggtcaccag
    56761 taatgccgtt gaagtgcatg tgcatcatct gcgacgcaaa ctcggtagtg attttattcg
    56821 taccgtgcat ggtattggct acacattagg tgagaaatga aatttaccca acgtcttagt
    56881 ctgcgcgtca ggctgacgct aatcttttta attctggcct cggtgacctg gctgctttcc
    56941 agctttgtcg cctggaaaca aacaacggat aacgtcgatg aattgttcga cacccaactg
    57001 atgctgtttg ccaaacggtt aagtacgctc gatctcaacg aaatcaacgc ggcggatcgc
    57061 atggcacaga cgccaaataa attaaaacac ggtcatgttg atgacgatgc gctgaccttt
    57121 gccatcttta cccacgacgg cagaatggtc cttaatgatg gcgataacgg agaagatatt
    57181 ccctatagct atcaacggga aggttttgct gacgggcaac tggtcggtga agacgatcct
    57241 tggcgttttg tctggatgac ctcacctgat ggcaaatatc gcatcgttgt tggccaggaa
    57301 tgggaatacc gtgaagacat ggcgctggcg attgttgccg ggcaattgat cccgtggctg
    57361 gtcgcactgc cgattatgtt aatcatcatg atggtactac tgggtcgtga actcgcgccg
    57421 ctgaacaaac tggcgctggc actacgtatg cgtgaccctg actcggaaaa accactaaac
    57481 gcgactggcg tacccagcga agtgcggcca ctggttgagt cgctaaatca actgttcgcc
    57541 cgcacacatg cgatgatggt tcgagaacga cgctttacct ccgatgcagc tcacgaacta
    57601 cgtagcccgt taacagcgct gaaagtgcaa accgaagttg cgcagctctc tgacgatgat
    57661 ccgcaggcgc ggaaaaaagc actgctccaa ttacattccg ggatcgatcg cgctactcgt
    57721 ctggttgatc aactgctcac gctatcgcgg ctggactcac tggataacct tcaggacgtc
    57781 gcggagatcc cgcttgaaga tctcctacaa tcgtcggtga tggatattta ccacacggcg
    57841 cagcaggcga aaattgacgt gcgactgaca ctcaatgccc acggcatcaa acgcaccgga
    57901 caaccgctat tgctaagttt gttggtgcga aatttgctgg ataacggccc cggtgtgaca
    57961 ccagaggcac tggcgcgaat tggcgaacgc ttctatcgcc cacccggaca aaccgctacc
    58021 ggcagtggac ttgggctatc gattgtccag cgaatcgcca agctgcatga catgaatgtt
    58081 gagtttggga atgcggaaca aggtggattt gaggcgaagg taagctggta acttctctgt
    58141 ctatgagagc cgttaaaacg actctcatag attttactaa tagcaaaata taaaccgtcc
    58201 ccaaaaaagc caccaaccac aaaccagaca aataacgagc gagaatacat atagccaaaa
    58261 ctcgaacatt tcccgcataa agagttttct taagataaga ataataagtg gcgtaagaag
    58321 aaaaaatgct gcaatcattg cttgcaatat acgatcttcg cctgaaacgg aaggtatact
    58381 gcaaatattg cctcccgact caagcatcca gtcatactta ttgaaagcaa aaaaagaatc
    58441 tattaaccaa taagtaagca ttatatatgg caatgcttca aatattgttt taattttctg
    58501 ctttaacata tatcagtacg ctcataaaaa tccatttata attatcaaca tgacacgttg
    58561 atattttcat cttttactta ttcaaagcca tattttcaat ccccaaaaaa accgaaccaa
    58621 acgtgtaaat tattccggtg cgagtaagaa ttatgaggaa tggctatcag tattgtcatt
    58681 ttcagaaaat atttatcctg catcggtgag tcagagctag atcagacttt tgctaaattc
    58741 gcaaaagact ttgcacattt tgctaatttc acagtaccgc tctgtgacgt actatagtcg
    58801 acaaacgtct caccttgaga ttaaaaaatg agcaacatcc tgattatcaa cggcgcgaaa
    58861 aaattcgccc actccaatgg tcaactgaac gacaccctga ccgaagtcgc ggatggcaca
    58921 ctgcgcgacc tcgggcatga tgtccgcatc gttcgcgccg acagcgacta caatgtcaaa
    58981 gcggaagtac aaaactttct ctgggctgat gtggtgatct ggcagatgcc aggctggtgg
    59041 atgggcgcgc cgtggacagt gaaaaaatat attgatgatg tattcaccga aggtcacggg
    59101 actctgtatg ccagcgatgg tcgtacccgc aaagatccgt cgaaaaaata cggttccggc
    59161 agcctggtac aggacaaaaa atatatgctt tctctaacct ggaacgcacc aatggaagcc
    59221 ttcaccgaaa aagatcagtt cttccacggc gttggcgttg acggtgtgta tctgccgttc
    59281 cataaagcaa accaattcct cggtatggaa ccgctgccga catttatcgc taatgacgtg
    59341 ataaaaatgc ctgatgttcc ccgctatact gaagaatatc gcaagcatct tgtggaaatt
    59401 tttggttaac tagagctcag gctttagaag gagttaacca tgcttaccgt aatcgcagaa
    59461 atccgtactc gtcctggtca acatcaccgt caggcggtat tggatcagtt tgctaaaatc
    59521 gttccaaccg tactgaaaga agaaggttgc cacggctatg cgccaatggt ggattgcgct
    59581 gctggcgtga gtttccagtc tatggcaccg gattctatcg tgatgattga gcagtgggaa
    59641 agcatcgcgc accttgaagc gcatctgcaa accccgcaca tgaaggcgta cagcgaagcc
    59701 gtaaaaggcg acgtgctgga gatgaatatc cgtattctgc agccagggat ttaatcctgc
    59761 cttgtttgcc cggccatcct gaccgggcaa tgttctttcc tttaaacctc aatctccgcc
    59821 atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg agcgtttctt cgccagcagc
    59881 atatccatca tcgcgtcagt acgctgatcg tcttcatcat cgatagtcaa ctgcaccaga
    59941 cggcgagtgt tcggatcaag cgtggtttca cgcaattgca tcgggttcat ttcccccaga
    60001 cctttaaaac gctggacgtt cggcttgcct ttcttgcgtt ttaattgctc aagtacgccc
    60061 tctttctctt cttccgtcag cgcgtaataa acctctttcc cgagatcaat acggtagagc
    60121 ggtggcagtg cgacgtaaac gtgaccgtgt ttcaccaacg cgcggaagtg ttttacgaac
    60181 aaagcgcaga gcagcgtggc aatgtgcaga ccatcagagt ccgcatccgc gaggatacag
    60241 attttgccat aacgaagctg gctcagatcg tcgctgtcag gatcgatacc gatcgctacc
    60301 gaaatatcat gcacttcctg cgaggccagc acttcgtcgg aagaaacttc ccaggtgtta
    60361 aggatcttac ctttcagcgg catgatcgcc tgatattcac gatcgcgcgc ctgcttggca
    60421 gatccgcctg cggagtcacc ttccacgagg aacagttcgg tacggttaag gccctgcgcg
    60481 gtacaatcag ccagtttgcc aggcagcgcc gggccgctgg tcagcttttt acgcaccact
    60541 tttttagccg cacgcatacg acgctgggcg ctggaaatcg ccatctctgc cagcaactcc
    60601 gccgcctgaa cgttctggtt cagccacagg atgaaggcat ctttcaccac gcccgaaacg
    60661 aatgccgcac actgacgcga agagagacgc tctttggtct gcccggcaaa ctgcggatcc
    60721 tgcattttaa ccgacagaac ataggcgcag cgatcccaga tatcttccgc cgacagcttc
    60781 acgccgcgcg gcagaatgtt gcggtattca cagaactcac gcatcgcgtc caacaggccc
    60841 tgacgcagac cattaacatg ggtaccgccc tgcatcgttg ggataaggtt gacgtagctt
    60901 tcggtcagca gttcaccgcc ttccggcagc cacagtagcg cccagtccac agcttcagta
    60961 tcgccagcga aattaccgat aaacggtttt tctggcagcg tcggtaaacc gtttaccgct
    61021 tccgccaggt aatcattcag accgtcctga tagcaccagc gctgttcggt gttgttgatc
    61081 tcatctttaa aggtaatctc aacgccgggg cacagtactg ctttggcctt cagcacatgc
    61141 gtcaggcgtg aaacagaaaa acgcgggctg tcaaagaagg tttcatccgg ccagaagtgc
    61201 acgctggtac cggtattgcg tttaccgcaa gtgccgacaa cctgtaaatc ctgcaccttt
    61261 tcgccatttt caaaggcgat gttataaacc tgaccatcgc ggcgcacgtt aacttctacg
    61321 cgcttcgaca aggcgttaac taccgaaatc cccacgccat gcaggccgcc agagaactgg
    61381 taatttttgt tagagaattt accgcctgca tgcagacggc aaagaatcag ttcaaccgcc
    61441 ggtactcctt cttccgggtg aatatccacc ggcatcccgc gcccatcgtc aatcacttct
    61501 aacgactggt cagcatgtaa gattacatcc acgcgttttg cgtgacccgc cagtgcttca
    61561 tcgacactgt tatcaataac ttcttgcccc aaatggttag ggcgagtggt atcggtatac
    61621 atccccggac ggcggcgaac cggctcaagc ccggtgagta cctcaatggc atcagcgtta
    61681 taagtttgcg tcatggttta agttagcaat tcgagttgat cgtcagagat ggtgcagacc
    61741 aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc gtgaatgcgt ggttgccgcc
    61801 ttctatgaca gtctggcggc aggaagcata gtacgccacc gcctggcggt aatccagcac
    61861 ttcatctccc gtctgttgca gcagccagat caaatccggc gcttccagcg ggtcaatctg
    61921 catgactttg agatcgtaaa tatggcgtga ctctagcaca tattgctgcc cggtgtaggg
    61981 gttctcgttc tgaccgagat agtccgtcag caattcaaac gggcgtaccg ccgggtttac
    62041 caccactgcg ggcagcataa aacattgcga caaccaggtg gcgtaatatc cccccagtga
    62101 cgaaccgaca atacccagcg aatcgccgcc atgttccagg acaatggatt ccagcagttc
    62161 tgccgcgtcg gaaggatacg gcggcaactg cggaatgatc atctcaacgt cagggtgatg
    62221 ttccgccagc cagtttttta acaagctcgc ttttgcagag cgcggcgagc tgttgaaacc
    62281 gtgtaaataa agaagcgtag acatcagtag ccttctgaag cggtatcagg ttggaaacgt
    62341 gtgtccgcca ggcgatgcac ctcggtggtc agcgtgccat cagcatgtaa ctcgagagta
    62401 cgccagccgg gcgcgatagt atccagcgta aagttggaac agtgcggctt aaactgcaca
    62461 caggtcgacg gcgttgccag caggcggcga ccgttccagt cgagatccag ctcctgatga
    62521 atatgaccgc acagcaagta tttgacgtgc ggaaacttcg ccagcacggt atccagttcg
    62581 cccgcgttac gcagactgtg ttgatcgagc caactacaac ccgcaggtag cggatgatga
    62641 tgcagcagca gcaacgtatg gcgttctggc gcatcggcca gtttacgttc cagccactca
    62701 agctgaaact cgctcagctc accgtgcggc acgccaaaca cctggctatc cagcaacagg
    62761 atttgccatt gctcaccaat aaacacccgc ttcgccgggg agatacccgc atcctgtaac
    62821 gcgctgtaca tcgcgggctg gaaatcgtgg ttgcccggca gccagacgca gggcgcacga
    62881 aaacttgcga tgccttcagc gaaatgctga taggccgcag aggattgatc ctgcgctaaa
    62941 tcacctgtcg cgacaatcag gtcgaattcg tgctggtgtg gacgaatcgc ctccagcacc
    63001 gcctggtagc tttcccaggt gtttacccct aacagggctt cgtgcttttg tgcaaacagg
    63061 tgagtatcgg taatttgtaa aatccttcct ctggcctcac cagccagagg aagggttaac
    63121 aggctttcca aatggtgtcc ttaggtttca cgacgctagt aaaccggaat cgccatcgct
    63181 ccatgtgcta aacagtatcg caaccagtcc gctaaaaact ggttaatttg atgcttttcg
    63241 tcgcgttgat gcaacttttt attaggataa tcataccgcg ctttgaagcg aaaaatctgc
    63301 tggcttgaac acacttcagc caccatcgcg tcatgataca gacgcaccgt cattgacgga
    63361 aggctccagt aactgatcgc gggcgcagtc tgttctattg tcaccagggt agtgtatcgg
    63421 gtcgattcca caatcgtcag ccgatattgt gcgtttgcca cctgatagct tacagtttcg
    63481 ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg aaaagttcat ctcgcacagg
    63541 cgcatcattt caggaaagtc aggtgtgtaa cgcttcattt atgcccactc attttttaac
    63601 gcttgatgat gcagctgcag ccattgcaaa gcgatgaccg acgctgcgtt gtcgattttc
    63661 ccctcttcta cccactggta tgcctgttcc cggcttacca catgaacgcg aatatcttcg
    63721 ttttcatcag ccagaccatg aataccgctt gcggtcgtgg cgtccacttc gcccaccata
    63781 attgacgaac gctcactggt gccccccggg cttgccagga aacttaacac cggtttggtc
    63841 cgtttgacta tcagtcccgc ctcttcaatc gcttcgcgac gggcaacatc ttccacactt
    63901 tcaccctctt caatcatccc ggcaaccatc tccagtagcc agggggtttc gctggtgtcg
    63961 tacgcggcaa tccgaatctg ctcaatcagc acaacttcat cacgcactgg gtcaaagggt
    64021 agcaagactg cggcgtgacc gcgctcaaaa atttcccgcc gtacctcatg actcatttgc
    64081 ccgttgaata gacgatgacg aaatctataa agatctaatg aaaaaaagcc gcgataaagt
    64141 gtttctcgtg caataatttc tacatcgttt ttgccaaatg taacgggcag gttgtctggc
    64201 ttaagcattg ttaatgtcct agcactaata gtgaattaaa tgtgaatttc agcgacgttt
    64261 gactgccgtt tgagcagtca tatgttaaat tgaggcacat taacgcccta tggcacgtaa
    64321 cgccaacctt ttgcggtagc ggcttctgct agaatccgca ataattttac agtttgatcg
    64381 cgctaaatac tgcttcacca caaggaatgc aaatgaagaa attgctcccc attcttatcg
    64441 gcctgagcct ttctgggttc agttcgttga gccaggccga gaacctgatg caagtttatc
    64501 agcaagcacg ccttagtaac ccggaattgc gtaagtctgc cgccgatcgt gatgctgcct
    64561 ttgaaaaaat taatgaagcg cgcagtccat tactgccaca gctaggttta ggtgcagatt
    64621 acacctatag caacggctac cgcgacgcga acggcatcaa ctcgaacgcg accagtgcgt
    64681 ccctgcagtt aactcaatcc atttttgata tgtcgaaatg gcgtgcgtta acgctgcagg
    64741 aaaaagcagc agggattcag gacgtcacat atcagaccga tcagcaaacc ttgatcctca
    64801 acaccgcgac cgcttatttc aacgtgttga atgctattga cgttctttcc tatacacagg
    64861 cacaaaaaga agcgatctac cgtcaattag atcaaaccac ccaacgtttt aacgtgggcc
    64921 tggtagcgat caccgacgtg cagaacgccc gcgcgcagta cgataccgtg ctggcgaacg
    64981 aagtgaccgc acgtaataac cttgataacg cggtagagca gctgcgccag atcaccggta
    65041 actactatcc ggaactggcg gcgctgaatg tcgaaaactt taaaaccgac aaaccacagc
    65101 cggttaacgc gctgctgaaa gaagccgaaa aacgcaacct gtcgctgtta caggcacgct
    65161 tgagccagga cctggagcgc gagcaaattc gccaggcgca ggatggtcac ttaccgactc
    65221 tggatttaac ggcttctacc gggatttctg acacctctta tagcggttcg aaaacccgtg
    65281 gtgccgctgg tacccagtat gacgatagca atatgggcca gaacaaagtt ggcctgagct
    65341 tctcgctgcc gatttatcag ggcggaatgg ttaactcgca ggtgaaacag gcacagtaca
    65401 actttgttgg tgccagtgag caactggaaa gcgcacatcg tagcgtcgtg caaaccgtac
    65461 gttcctcctt caacaacatt aatgcttcta tcagtagtat taacgcctac aaacaagccg
    65521 tagtttccgc gcaaagctca ttagacgcga tggaagcggg ctactcggtc ggtacgcgta
    65581 ccattgttga tgtgttggat gcgaccacca cgctgtacaa cgccaagcaa gagctggcga
    65641 atgcgcgtta taactacctg attaatcagc tgaatattaa gtcagccctg ggtacgttga
    65701 acgagcagga tttgctggca ctgaacaatg cgctgagcaa accggtttcc actaatccgg
    65761 aaaacgttgc cccgcaaacg ccggaacaga atgctattgc tgatggttat gcgcctgata
    65821 gcccggcacc cgtcgttcag caaacatccg cacgcactac caccagtaac ggtcataacc
    65881 ctttccgtaa ctgatgacga cgacggggct tcggccccgt ctgaacgtaa ggcaacgtaa
    65941 agatacgggt tatctgccgc attcttcccc cttctcgctt caatttcgac cagccatcct
    66001 ctattctgat gggtatttac cactggtccc ggaagacaaa aatgaaacgg acaaaatcca
    66061 tacgccacgc atcgttccgc aaaaactgga gcgcacgcca tctgacacca gtcgctctcg
    66121 cggttgccac tgtttttatg ctggctggct gtgaaaagag tgatgaaaca gtgtctctct
    66181 atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa aagcgccgaa tgtaccaccg
    66241 cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc gaaatacgcc acccgtgaag
    66301 actgtgttgc tgaatttggt gaaggtcagt gtcagcaagc accagcccag gctggcatgg
    66361 caccagaaaa ccaggcgcag gctcagcaat ccagcgggag tttctggatg ccgctgatgg
    66421 ccggttacat gatggggcgt ctgatgggcg gcggcgcggg atttgcacag cagccgctgt
    66481 tctcctcgaa aaacccggcc agtccggctt acggtaaata taccgacgcg acgggtaaaa
    66541 actatggcgc agcccagcca ggccgcacca tgaccgtacc gaagacggca atggcaccaa
    66601 aaccggcgac caccactacc gttacccgtg gcggttttgg tgaatctgtt gccaaacaaa
    66661 gcactatgca gcgtagcgca accggcacct cttctcgttc aatgggtggc tgataccgat
    66721 ggaaagagtc agtattaccg agcgcccgga ctggcgtgag aaagcccacg aatacggttt
    66781 caattttcac accatgtacg gcgagccgta ctggtgtgaa gatgcttact acaagttgac
    66841 cctcgcccag gttgaaaagc tggaagaagt caccgccgaa ctgcaccaga tgtgcctgaa
    66901 agtggtggaa aaagtgatcg ccagcgatga gctgatgacc aaattccgca taccaaaaca
    66961 cacctggagt tttgtgcgtc aatcatggct gactcaccag ccatcgcttt attcgcgtct
    67021 tgatctggcg tgggatggca ctggcgaacc taaacttctg gaaaataacg ccgatacgcc
    67081 aacgtcacta tacgaggcgg cgttctttca gtggatctgg ctggaagatc agcttaacgc
    67141 cggtaacttg ccggagggca gcgaccagtt taacagtctg caagaaaaac tgatcgatcg
    67201 cttcgttgag ctgcgtgaac agtatggctt ccagttgctg catctcacct gctgtcgcga
    67261 cacggtggaa gatcgcggaa ccattcagta tttgcaggac tgcgcaaccg aagctgaaat
    67321 cgccaccgag ttcctctaca tcgacgagat aggtttaggc gaaaaaggcc agttcacgga
    67381 tttacaggat caggtgattt ctaacctgtt caagctttat ccgtgggaat ttatgctgcg
    67441 cgaaatgttc tccaccaagc tggaagatgc tggcgtacgc tggctggaac cggcgtggaa
    67501 gagcattatc tccaacaagg cacttctacc gctactgtgg gagatgttcc cgaatcaccc
    67561 gaacctgctg cccgcttatt ttgcggaaga tgatcatccg caaatggaaa aatatgtggt
    67621 taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc attgagaacg gcaaaaccat
    67681 tgaagcagcg gaaggtccgt atggcgaaga agggatgatt gttcagcaat tccacccgtt
    67741 accgaaattc ggcgacagct atatgctgat tggtagctgg ctggtgaacg atcaacctgc
    67801 cggaattggc attcgtgaag accgtgcgtt gatcacccag gatatgtctc ggttttatcc
    67861 acatattttt gttgaataag ccacgatacc ggatggcact cgccatccgg taattgttag
    67921 cctatctgca ccgacagcat acttaaactg cccatttcta taccatcgac cggaatggta
    67981 attggctcct gcccatccca cacacctaac acatacaaca acggcagata gtgctctggc
    68041 gttgggttcg ataacgcgcc accttcatgg tcgagataat tcaccagagg atgctgctct
    68101 accggccctt gccacgtcag attcgctttc acatactcat taaacgacgt cgcccacgga
    68161 tacggtgaac tatcaccgtg ccacttcact gtgcgcaggt tatgcaccac gttaccgctg
    68221 gcgaccaaca ttattccttc atcacgcagc gccgcgagtt tgcgccccat ttcgaaatgc
    68281 caggcggcag gtttgctact gtcgatactc aactgcacca tagggatatc ggcgtcagga
    68341 tacatcttaa tcagcacgcc ccacgagccg tggtcaaagc cccaggcttc tttatccagc
    68401 gtcaccggga tcggcgctaa cagctcaacc agacgctgtg ccagcgcagg cgaacccgga
    68461 gcaggataat gcgtatcgta cagcgcctgc gggaagccac caaagtcatg aatcgtgggc
    68521 ggcgtctcca tcgcggtcac tcctgttcca cgggtaaacc agtgagccga aaccaccaca
    68581 atcgcttgcg ggcgtggcaa tgtcatcccc aacttctgcc agctgcgggt atacaaatta
    68641 tcttccagca cgttcatcgg actaccgtga cctaaaaaca atgctggcat acgtgttgaa
    68701 gacatgatga tatccttaac taaaggtgtc attttgatat cctcacaata cgcttgttcg
    68761 gcggagtaag aacccggata acaatgatga tgatcatcag ttattttgac gatctgcctg
    68821 aaggtgaaga tttataagga gttgtcgatg tcagtacctc tcattctgac catactggcg
    68881 ggggcagcca cgtttattgg cgcgtttctc ggcgttctcg gacaaaaacc ctcgaaccgc
    68941 ttactggcgt tttcgctagg ttttgcggcg gggatcatgt tgctcatctc attaatggaa
    69001 atgcttcctg ccgcactagc agctgaagga atgtcgccgg tgttgggtta tggaatgttt
    69061 atcttcggtc tgcttggcta ctttggcctg gaccgcatgt tgccgcatgc gcatccgcag
    69121 gatttaatgc aaaaatcggt gcagctgttg ccaaaatcga tcaagcgcac agccattctg
    69181 ctcactctcg gcatcagtct gcataacttc ccggaaggga ttgccacctt tgtcacggcg
    69241 agcagcaacc tggagctggg atttggcatc gcactggccg tcgcgttgca caatatccct
    69301 gaaggtctgg cagtggcagg cccggtttat gcggcaacgg ggtctaaacg caccgcgatt
    69361 ctgtgggcgg ggatttctgg actagcagaa attcttggtg gtgtgctggc gtggttaatc
    69421 ctcggtagca tgatttcccc ggtggtcatg gcggcaatca tggcggcggt tgcaggaatt
    69481 atggtggcgc tctcggttga tgaattaatg ccgctcgcca aagagattga ccctaataat
    69541 aaccccagct atggcgtact atgtggaatg tcagtgatgg gattcagttt agtgctgcta
    69601 caaacggcgg gaattggtta aaaacaacat cagaagttgt ccagcacatg gacaacttct
    69661 gccagcttca cgaaagcctt attctacctt ttcggctgtc tcttctctcg tactgtttaa
    69721 gtatttgttc cgcgcttcaa gcagtgcgtc tctgtcctgg cgaaacgccc gacaataacg
    69781 tttcaaatgc aggaaataac ccaccacaac cagagcaatc aatacaatcc agtaccaggc
    69841 gataaacatc cttttaccct taatatttaa caatactaaa catttagcgc ataaatttca
    69901 catatccttt ttcggatata ttcatatggt cgcataataa cggaacaatt atatgatttt
    69961 gatcataatg aaaaagaatg cctgatatta atgaaatttt ctcatagaaa gagaagcgga
    70021 attaacatct tgcatagcag ggcaaaataa aaaccgaagc cagctacaac ttcggtttct
    70081 tgattaaggc agtaattaag cagcggtttt cagctggctt tacgctcatg tgcctgacgg
    70141 tatgccacca ggtcttcaat agtcacgagc gccatattgt gtttattggc aaactcaatg
    70201 cactctggtg cacgcgccat cgtgccatcg tcattagtca gctcacacag cacaccagcc
    70261 ggtttaaagc ctgccagcgt catcaaatca atagtcgctt cagtatgacc gccacgcgtc
    70321 agtacaccac ctgcctgcgc gcgaagtggg aaaacgtggc ccgggcgatt cagatcagaa
    70381 ggttttgcgc catcggcaat cgctgcgcga acggtcgtaa tacgatcagc ggcagaaaca
    70441 ccggtagtca caccttcagc cgcttcaatg gtcacggtaa aaccggtgcc ataggcgctg
    70501 gtgttatttt ctaccatcat tggcagatca agttgtttac ggcgatcttc agtgatgcac
    70561 aggcaaacaa taccgctacc gtggcgaatg gtcagcgcca tctgctcaac ggtcatggtt
    70621 tctgccggga agatcatatc gccttcgttt tcacggtctt catcatcaag caccattaca
    70681 ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac gttcgaaagg cgtaccaaaa
    70741 gaggaaagta gcgtctgatt catggtaaaa aaacctcact aaaattatgg ttaccagaat
    70801 cagggcagtc ttaggagtgg cggcatatag ccaaaataac gtgagcgggt ccatgcccga
    70861 cagaatcgtt actctctccc atccggactc taaccgtcgg ccccggaatt acaccggatc
    70921 tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt tcaactgagt tgatttaccg
    70981 ccggtgggga atttcgcccc gccctgagaa taagcggatt cactataacg ctaatgatta
    71041 gcggcagcaa cgcatagctt cacataattc tggtttatga cttaccctta tcgcactaca
    71101 atggtactca acacctatca gtacagggaa actacaatga ttgacccgaa aaaaattgag
    71161 caaatcgctc gccaggttca cgaatcaatg cctaaaggaa tcagggagtt cggggaagat
    71221 gtggagaaaa aaatccgcca aaccctacaa gctcagctga cgtgcctcga ccttgtaagc
    71281 cgtgaagagt tcgacgtcca aacgcaagtc ctgttacgga ctcgtgagaa actggcgctg
    71341 cttgaacaaa gaatcagcga actggaaaat cgcagtactg agatcaaaaa gcagccagat
    71401 ccagagac
//
LOCUS       DAJTUP010000005        66961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTUP010000005 DAJTUP010000000
VERSION     DAJTUP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.005"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO468_000340"
     CDS             complement(66..1070)
                     /gene="cra"
                     /locus_tag="OO468_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR6257588.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1152..1238)
                     /locus_tag="OO468_000341"
     CDS             complement(1152..1238)
                     /locus_tag="OO468_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR6257589.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO468_000342"
     CDS             complement(1250..1741)
                     /gene="ilvN"
                     /locus_tag="OO468_000342"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6257590.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO468_000343"
     CDS             complement(1744..3468)
                     /gene="ilvI"
                     /locus_tag="OO468_000343"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR6257591.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3587..3631
                     /locus_tag="OO468_000344"
     CDS             3587..3631
                     /locus_tag="OO468_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257592.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO468_000345"
     CDS             complement(3786..4730)
                     /gene="leuO"
                     /locus_tag="OO468_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR6257593.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5389..5475
                     /gene="leuL"
                     /locus_tag="OO468_000346"
     CDS             5389..5475
                     /gene="leuL"
                     /locus_tag="OO468_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR6257594.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5568..7139
                     /gene="leuA"
                     /locus_tag="OO468_000347"
     CDS             5568..7139
                     /gene="leuA"
                     /locus_tag="OO468_000347"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR6257595.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7139..8230
                     /gene="leuB"
                     /locus_tag="OO468_000348"
     CDS             7139..8230
                     /gene="leuB"
                     /locus_tag="OO468_000348"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR6257596.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8233..9633
                     /gene="leuC"
                     /locus_tag="OO468_000349"
     CDS             8233..9633
                     /gene="leuC"
                     /locus_tag="OO468_000349"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR6257597.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9644..10249
                     /gene="leuD"
                     /locus_tag="OO468_000350"
     CDS             9644..10249
                     /gene="leuD"
                     /locus_tag="OO468_000350"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR6257598.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO468_000351"
     CDS             complement(10298..11476)
                     /gene="setA"
                     /locus_tag="OO468_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR6257599.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO468_000352"
     CDS             complement(11578..11709)
                     /gene="sgrT"
                     /locus_tag="OO468_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR6257600.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11798..13453
                     /gene="sgrR"
                     /locus_tag="OO468_000353"
     CDS             11798..13453
                     /gene="sgrR"
                     /locus_tag="OO468_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR6257601.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13487..13586
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13617..14600
                     /gene="thiB"
                     /locus_tag="OO468_000354"
     CDS             13617..14600
                     /gene="thiB"
                     /locus_tag="OO468_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR6257602.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14576..16186
                     /gene="thiP"
                     /locus_tag="OO468_000355"
     CDS             14576..16186
                     /gene="thiP"
                     /locus_tag="OO468_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR6257603.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16170..16868
                     /gene="thiQ"
                     /locus_tag="OO468_000356"
     CDS             16170..16868
                     /gene="thiQ"
                     /locus_tag="OO468_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR6257604.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16982..17746)
                     /locus_tag="OO468_000357"
     CDS             complement(16982..17746)
                     /locus_tag="OO468_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6257605.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO468_000358"
     CDS             complement(17832..18710)
                     /gene="araC"
                     /locus_tag="OO468_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR6257606.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19049..20749
                     /gene="araB"
                     /locus_tag="OO468_000359"
     CDS             19049..20749
                     /gene="araB"
                     /locus_tag="OO468_000359"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR6257607.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20760..22262
                     /gene="araA"
                     /locus_tag="OO468_000360"
     CDS             20760..22262
                     /gene="araA"
                     /locus_tag="OO468_000360"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR6257608.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22462..23157
                     /gene="araD"
                     /locus_tag="OO468_000361"
     CDS             22462..23157
                     /gene="araD"
                     /locus_tag="OO468_000361"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR6257609.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23232..25583
                     /gene="polB"
                     /locus_tag="OO468_000362"
     CDS             23232..25583
                     /gene="polB"
                     /locus_tag="OO468_000362"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR6257610.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25748..28654
                     /gene="rapA"
                     /locus_tag="OO468_000363"
     CDS             25748..28654
                     /gene="rapA"
                     /locus_tag="OO468_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR6257611.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28666..29325
                     /gene="rluA"
                     /locus_tag="OO468_000364"
     CDS             28666..29325
                     /gene="rluA"
                     /locus_tag="OO468_000364"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR6257612.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO468_000365"
     CDS             complement(29442..30257)
                     /gene="djlA"
                     /locus_tag="OO468_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR6257613.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30512..32866
                     /gene="lptD"
                     /locus_tag="OO468_000366"
     CDS             30512..32866
                     /gene="lptD"
                     /locus_tag="OO468_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR6257614.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32919..34205
                     /gene="surA"
                     /locus_tag="OO468_000367"
     CDS             32919..34205
                     /gene="surA"
                     /locus_tag="OO468_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR6257615.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34205..35194
                     /gene="pdxA"
                     /locus_tag="OO468_000368"
     CDS             34205..35194
                     /gene="pdxA"
                     /locus_tag="OO468_000368"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR6257616.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35191..36012
                     /gene="rsmA"
                     /locus_tag="OO468_000369"
     CDS             35191..36012
                     /gene="rsmA"
                     /locus_tag="OO468_000369"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR6257617.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36015..36392
                     /gene="apaG"
                     /locus_tag="OO468_000370"
     CDS             36015..36392
                     /gene="apaG"
                     /locus_tag="OO468_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR6257618.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36399..37241
                     /gene="apaH"
                     /locus_tag="OO468_000371"
     CDS             36399..37241
                     /gene="apaH"
                     /locus_tag="OO468_000371"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR6257619.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO468_000372"
     CDS             complement(37319..37798)
                     /gene="folA"
                     /locus_tag="OO468_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR6257620.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO468_000373"
     CDS             complement(37989..39851)
                     /gene="kefC"
                     /locus_tag="OO468_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR6257621.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWVKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO468_000374"
     CDS             complement(39844..40374)
                     /gene="kefF"
                     /locus_tag="OO468_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR6257622.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40422..41816)
                     /locus_tag="OO468_000375"
     CDS             complement(40422..41816)
                     /locus_tag="OO468_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6257623.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO468_000376"
     CDS             complement(41875..42162)
                     /gene="fixX"
                     /locus_tag="OO468_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR6257624.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42159..43445)
                     /locus_tag="OO468_000377"
     CDS             complement(42159..43445)
                     /locus_tag="OO468_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6257625.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43496..44437)
                     /locus_tag="OO468_000378"
                     /pseudo
     CDS             complement(43496..44437)
                     /locus_tag="OO468_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44452..45222)
                     /locus_tag="OO468_000379"
     CDS             complement(44452..45222)
                     /locus_tag="OO468_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR6257626.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45578..47092
                     /gene="caiT"
                     /locus_tag="OO468_000380"
     CDS             45578..47092
                     /gene="caiT"
                     /locus_tag="OO468_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR6257627.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47123..48265
                     /gene="caiA"
                     /locus_tag="OO468_000381"
     CDS             47123..48265
                     /gene="caiA"
                     /locus_tag="OO468_000381"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR6257628.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48394..49611
                     /gene="caiB"
                     /locus_tag="OO468_000382"
     CDS             48394..49611
                     /gene="caiB"
                     /locus_tag="OO468_000382"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR6257629.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49685..51238
                     /gene="caiC"
                     /locus_tag="OO468_000383"
     CDS             49685..51238
                     /gene="caiC"
                     /locus_tag="OO468_000383"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR6257630.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51347..52132
                     /gene="caiD"
                     /locus_tag="OO468_000384"
     CDS             51347..52132
                     /gene="caiD"
                     /locus_tag="OO468_000384"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR6257631.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52138..52728
                     /gene="caiE"
                     /locus_tag="OO468_000385"
     CDS             52138..52728
                     /gene="caiE"
                     /locus_tag="OO468_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR6257632.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52814..53209)
                     /gene="caiF"
                     /locus_tag="OO468_000386"
     CDS             complement(52814..53209)
                     /gene="caiF"
                     /locus_tag="OO468_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR6257633.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53471..56692)
                     /gene="carB"
                     /locus_tag="OO468_000387"
     CDS             complement(53471..56692)
                     /gene="carB"
                     /locus_tag="OO468_000387"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR6257634.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56710..57858)
                     /gene="carA"
                     /locus_tag="OO468_000388"
     CDS             complement(56710..57858)
                     /gene="carA"
                     /locus_tag="OO468_000388"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR6257635.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58314..59135)
                     /gene="dapB"
                     /locus_tag="OO468_000389"
     CDS             complement(58314..59135)
                     /gene="dapB"
                     /locus_tag="OO468_000389"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR6257636.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59302..60216)
                     /gene="rihC"
                     /locus_tag="OO468_000390"
     CDS             complement(59302..60216)
                     /gene="rihC"
                     /locus_tag="OO468_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR6257637.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60282..61232)
                     /gene="ispH"
                     /locus_tag="OO468_000391"
                     /gene_synonym="lytB"
     CDS             complement(60282..61232)
                     /gene="ispH"
                     /locus_tag="OO468_000391"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR6257638.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61234..61683)
                     /gene="fkpB"
                     /locus_tag="OO468_000392"
     CDS             complement(61234..61683)
                     /gene="fkpB"
                     /locus_tag="OO468_000392"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6257639.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61770..62264)
                     /gene="lspA"
                     /locus_tag="OO468_000393"
     CDS             complement(61770..62264)
                     /gene="lspA"
                     /locus_tag="OO468_000393"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR6257640.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62264..65080)
                     /gene="ileS"
                     /locus_tag="OO468_000394"
     CDS             complement(62264..65080)
                     /gene="ileS"
                     /locus_tag="OO468_000394"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR6257641.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65123..66064)
                     /gene="ribF"
                     /locus_tag="OO468_000395"
     CDS             complement(65123..66064)
                     /gene="ribF"
                     /locus_tag="OO468_000395"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR6257642.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66072..66290)
                     /locus_tag="OO468_000396"
     CDS             complement(66072..66290)
                     /locus_tag="OO468_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR6257643.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66393..66656
                     /gene="rpsT"
                     /locus_tag="OO468_000397"
     CDS             66393..66656
                     /gene="rpsT"
                     /locus_tag="OO468_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR6257644.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16240 a        18232 c        17243 g        15246 t
ORIGIN      
        1 gatgtccagt cccgtactct acgcgccaga gggaaattca cctggcgcgt atttttgttc
       61 gcggattagc tacggctgag cacgccgcgg cgatagagat tacgtttaat gcgcgttaaa
      121 ccaggttttg gcttacgcgg ttcgtccagg cttgccagga caatctccag cacacgctct
      181 gcgacatcgc ggtgacgttg agccactgcc agcaccggac actgtaagaa gtcgagcagt
      241 tcgttatcgc caaaggtggc aattgccagg tcagaaggca gtttgccgtc gcgacgcagc
      301 gtgacatcca tcactccttg caacaacgca aacgacgttg tgaacagcgc ctgcggcatc
      361 ggatgcgttt ccagccattt ttcgaataac tgggcagccg cctcccgctc atagctgttg
      421 gcatacagga aatgcacttc gcgcggatca tctttccaag cagtacggaa accttgttca
      481 cgcaggaagc tgacagaaag ctccggcagc gcgccaagat aaagcaccgt ctcggcggga
      541 aacttacgta actcttccgc cagcatttcg gcatcatcct gatcggcacc aaccacgctg
      601 gtgaaatgtt cacgatcgag ggcgcggtcc agcgcgacaa tcgggaacgg gtcgttagcc
      661 cagcgttgat aaaaaggatg ctcaggtggc aacgacgtcg aaacaataat ggcatcaacc
      721 tgacgctgta aaaggtgctc aatgcagcgc atttcgttgt ctggctgatc ttctgagcag
      781 gcaatcagca gttgataacc ccgttgccgc gcctggcgtt caagatagtt agcgatgcgg
      841 gtatagctgg tgttctccag atcggggatc acaagaccaa tagaacgtgt gcgtccagca
      901 cgaagcccag ctgccacggc gttcgggtgg taattgtgct cacgcaccac agccatgact
      961 ttttcaacgg ttttgtcgct cacacggtat tgcttcgctt tgccgttaat aacatagctt
     1021 gcagtggtcc gcgacactcc cgccagccga gcgatttcat ccagtttcac aattgcccct
     1081 tgcgtaaaat gtaaaaacca tagccattgt actggtatgg gttaaattca ttaacatcta
     1141 agcgcagaaa attaactgcg gcaactgctt ttattccagg ttcccactga tttcgcaaaa
     1201 aaagcccaac gtgacatgtt gggctgcaaa ttgcgcactg agatcattct caacgcatta
     1261 ttttatcgcc gcgcgaaagt ccgaccacac cagagcgagc aacttccaca attttcgcca
     1321 catcgcgaat cgatgctaaa aatgcatcaa gcttatcgct ggtgcctgct aattgaacgg
     1381 tataaagcga gggggtgaca tcgataattt gcccacggaa tatttccgta ttacgtttca
     1441 cttcgtcacg cccgtaaccg ctggcctgaa ttttcaccag catgatttcc cgctcaacat
     1501 gcgcgccctg ccccaactca ctcacgcgca agacatcgac cagcttgtgt aattgctttt
     1561 cgatctgctc aagtactttt tcatcgccca cggtctggat ggtcatacgc gataatgtcg
     1621 gatcgtcggt tggcgcaacg gtcaggcttt caatgttgta gcctcgctgg gaaaaaaggc
     1681 caatcacgcg ggataacgcg cctgattcat tttcgagtaa gactgataat atccggcgca
     1741 taatcaggtt ctctccgttt tgcttaacca catttcatcc attccgcccc cgcgaatctg
     1801 catcgggtag acgtgctcac tgccatcgac ggtaacatca acaaacacca ggcgattatt
     1861 gcgcacctgt tccagcgcct cgctaagttt gctttccagc tcatgcggat gagagatctg
     1921 gatcccgacg tgaccatagg cttccgccag acggacgaaa tcgggtagtg attgcatata
     1981 agattgtgaa tgacggccgg aatagatcat gtcctgccac tgtttcacca tccccagata
     2041 gcggttattg agattcacca ccagtacggg caactcgtat tgcaacgcgg tagacagttc
     2101 ctggatgttc atctgaatac tgccgtcgcc agtgacgcaa accacggttt cttctggcaa
     2161 cgccattttg acgcccagtg ccgcaggtaa accaaaaccc atcgtgccga gaccgccgga
     2221 attgatccag cgacgcggtt tgtcgaatgg ataataaagc gcagcaaaca tctggtgctg
     2281 cccgacatcg gacgtcacgt aagcgtcccc attagtcaac cgccaaagag tctcgatcac
     2341 cgcctgcggt ttaatctttt cactgtgagt gtcatatttc aggcactgaa gagcgcgcca
     2401 ctgttcaatc tgctgccacc agtcgcggat ctcatccagc ggttgatggg cggattcttg
     2461 cgacaggagt tcaagcattt gttcgaggac ctggcgagca tcccccacaa tcgggatatc
     2521 cgcagtcacg gttttagaaa tggaagtagg atcaatatcg atatgcagaa cagtggcatt
     2581 tgggcagtac tttgccagat tgttcgtcgt tcggtcatca aatcgtaccc cgacggcgaa
     2641 aatcacatcc gcgttatgca tcgtcatatt ggcttcgtag gtaccgtgca ttcccagcat
     2701 gcccagtgcc tgacgatgcg ttgccggaaa cgcccccatt cccatcaatg aggaaacaac
     2761 gggcagattc aacgcctcca ccgtttcttt caactgctga tggcagcccg ccatgattgc
     2821 cccaccgcct acgtagacaa ccggtttttt tgccgctacc agcgtttgca gagcacgctt
     2881 aatttgccct ttatgtccgg tagtagtggg attgtaagaa cgcatactga ccgactcagg
     2941 gcagacatag ggtaatttgt tcgccggatt aagaatatct ttcggtaaat caacgaccac
     3001 tggtcctggg cgaccacttg ccgccagcca gaaagccttt ttcagcacct gcggaatgtc
     3061 ttccgtttgc ttaaccagaa aactgtgttt aaccaccggt cgcgaaatcc ccaccatgtc
     3121 gcactcctga aaggcatcgt aacctatcaa cgaggtcgct acctgcccgg aaaggacaac
     3181 taatggaatg gaatccatat aagcggtggc gatgccggta atcgcattgg tcgcccttgg
     3241 acccgacgtt accagcacga cgccgacttc cccggtcgca cgcgccaggc catcggccat
     3301 atgcaccgcc gcctgctcat gacgaactaa tacatgatca ataccaccca cggtatgcaa
     3361 tgcatcgtaa atatcaagga ctgcgcctcc gggataaccg aatacttgtt taacgccctg
     3421 atcgataagc gatcggacga ccatctcggc tccagacaac atctccatgg cctgcctcac
     3481 tgtttgacgg aaaaaatgtg taaaacacat taaccgtccg atcttgctta agcaattggc
     3541 agccagagac ataaacgcag ggtaataaga ataaacagga ggaaaggtga aaagaaacga
     3601 taaaatcctc cattacgaag gattaaattg ataataaatc ggctgaatcc cacaacttac
     3661 agcaatttct ctattttttt aattaatcct aaaatagaga gtccacacat tcagaataaa
     3721 attctacatt caataaaaaa tgcagaataa accagacatt catgtctggc ctattctgca
     3781 ttcagttagc gtttgcaaat tgagactaat tgttcttcca tccactgatg gcctttatcg
     3841 cgcccggcag cttcatgcca agagagataa caggttctgc tgttttgttt taacggcagc
     3901 ggtaatacct gtaattctaa ggattcagcg aactcttcag ccagccaacg cggcgcaata
     3961 gcgaccaaat gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac
     4021 gcgatactgg cttgcttatc taccgtgtca taccaaggtt gactaaatga cgcgaaacga
     4081 tcgagcgaaa ccgccgcatg ttgttcgtta taaacatcat gtttcagtaa cgggccttta
     4141 attgttggat gatttttgct ggctaccagc accatttcat ctttaaataa tggcacgctg
     4201 gtaaattcag gacgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc
     4261 agctgatgtt cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca
     4321 atctgctcaa tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca
     4381 caaagatgaa atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt
     4441 tgtactagtt gcaatgcctg gcgaactgaa ccaaaaagtt gaaatgcgcg agcagtcggt
     4501 tgaataccac ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca
     4561 acagcgttac tgaccgcagg ttgcgacata cccagaacat gagcggcacg ggtaatgttt
     4621 tgctcctgca tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt
     4681 ggcttgctta actccgccgt ctctgaatga tctgtttgta cctctggcat acttaactcc
     4741 actgtcacac ttaactccct ttcccttatt ggaatgcaga gaaataatcc tgaagaatat
     4801 gatttatcat gcatataaaa taagaaaaag caaaatgagt aaaattcgga aaacataaag
     4861 atacgggttc tgataaaaac agaatccatt gccgtggaat gagtcattta ccacagcata
     4921 ataatccata atgttttgtt gcgaaaacaa tctaatcata actaccgcga atactcaatc
     4981 atctacaaaa tggattaaat gtgcttattt aataattaat ttatatattt aatgcattaa
     5041 ttcttaacat taattgttca ataatattca ccaaatcaat atcaaaaaaa atcgcaaaac
     5101 atataattca atacaaatca tcaggatagg ttttgcaacg cgtgcatttt gtcccctttt
     5161 tcctcgttga ttagatgcaa aaatttatgc tgaaatatgt caaccgatga aaagcgtcgg
     5221 tagttaagca gaaattaata tcgcttactt taaccaccgt agcacaatta gctaatttta
     5281 cggatgcaga actcacgctg gcgggacgtt tttattgcgt cagggttgac atccgttttt
     5341 gtatccagta actctaaaag catatcgcat tcatctggag ctgatttaat gactcacatc
     5401 gttcgcttta tcggtctact actactaaac gcatcttctt tgcgcggtag acgagtgagc
     5461 ggcatccagc attaagccag cacgcagtca aacaaaaaac ccgcgccatt gcgcgggttt
     5521 ttttatgccc gaagcgaggc gctctaaaag agacaaggac ccaaaccatg agccagcaag
     5581 tcattatttt cgataccaca ttgcgcgacg gtgaacaggc gttacaggca agcttgagtg
     5641 tgaaagaaaa actgcaaatt gcgctggccc ttgagcgtat gggtgttgac gtgatggaag
     5701 tcggtttccc cgtctcttcg ccgggcgatt ttgaatcggt gcaaaccatc gcccgccagg
     5761 ttaaaaacag ccgcgtatgt gcgttagctc gctgcgtgga aaaagatatc gacgtggcgg
     5821 ccgaatccct gaaagtcgcc gaagccttcc gtattcatac ctttattgct acctccccaa
     5881 tgcacatcgc caccaagctg cgcagcaccc tagacgaggt gatcgaacgc gctatctata
     5941 tggtgaaacg cgcccgtaat tacaccgatg atgttgaatt ttcttgcgaa gatgccgggc
     6001 gtacacccat tgccgatctg gcgcgagtgg tcgaagcggc gattaatgcc ggtgccacca
     6061 ccatcaacat tccggacacc gtgggctaca ccatgccgtt tgagttcgcc ggaatcatca
     6121 gcggcctgta tgaacgcgta cctaacatcg acaaagccat tatttccgta catacccacg
     6181 acgatttagg cctggcggtc ggcaactcac tggcggcggt acatgccggt gcacgccagg
     6241 tggaaggtgc aatgaacggg atcggcgagc gtgccggtaa ctgttcgctg gaagaagtca
     6301 tcatggcgat taaagttcgt aaggatattc tcaacgtcca caccgccatt aatcaccaag
     6361 agatatggcg caccagccag ttagttagcc agatttgtaa tatgccgatc ccggcaaaca
     6421 aagccattgt tggcagcggc gcattcgctc actcctccgg tatccaccag gatggcgtgc
     6481 tgaaaaaccg cgaaaactac gaaatcatga caccagaatc tattggtctg aaccaaatcc
     6541 agctgaatct gacctctcgt tcggggcgtg cggcggtgaa acatcgcatg gatgagatgg
     6601 ggtataaaga aagtgaatat aatttagaca atttgtacga tgctttcctg aagctggcgg
     6661 acaaaaaagg tcaggtgttt gattacgatc tggaggcgct ggccttcatc ggtaagcagc
     6721 aagaagagcc ggagcatttc cgtctggatt acttcagcgt gcagtctggc tctaacgata
     6781 tcgccaccgc cgccgtcaaa ctggcctgtg gcgaagaagt caaagcagaa gccgccaacg
     6841 gtaacggtcc ggtcgatgcc gtctatcagg caattaaccg catcactgac tataacgtcg
     6901 aactggtgaa atacagcctg accgccaaag gtcacggtaa agatgcgctg ggtcaggtgg
     6961 atattgtcac caactacaac ggtcgccgct tccacggcgt cggcctggcc accgatattg
     7021 tcgagtcctc cgccaaagcc atggtgcacg tactgaacaa tatctggcgt gccgcagaag
     7081 tcgaaaaaga gttgcaacgc aaaactcaac acaacgaaaa caacaaggaa accgtgtgat
     7141 gtcgaagaat taccatattg ccgtattgcc gggagacggt attggtccgg aagtgatgac
     7201 ccaggcgctg aaagtgctga atgccgtgcg caaccgcttt gcgatgcgca tcaccaccag
     7261 ccattacgat gtaggcggcg cagccattga taaccacggg caaccactgc cgcctgcgac
     7321 ggttgaaggt tgtgagcaag ccgatgccgt gctgtttggc tcggtaggcg gcccgaagtg
     7381 ggaacattta ccaccagacc agcaaccaga acgcggcgcg ctgctgccgc tgcgtaagca
     7441 cttcaaatta ttcagcaacc tgcgcccggc aaaactgtat caggggctgg aagcattctg
     7501 tccgctgcgt gcagacattg ccgctaacgg cttcgacatc cagtgcgtgc gcgaactgac
     7561 cggcggcatc tatttcggtc agccaaaagg ccgcgaaggt agcggacaat atgaaaaagc
     7621 gtttgatacc gaggtgtatc accgttttga gatcgagcgt attgcccgca tcgcgtttga
     7681 atctgctcgt aaacgtcgcc acaaagtcac ctcaatcgac aaagctaacg tgctgcaatc
     7741 ctctatttta tggcgtgaaa tcgtcaacga gatcgccacg gaatacccgg atgtcgaact
     7801 ggcgcatatg tacatcgaca acgccaccat gcagctgatt aaagatccat cacagtttga
     7861 cgttctgctg tgctccaacc tgtttggcga cattctgtct gacgagtgcg caatgatcac
     7921 tggctcgatg ggcatgttgc cttccgccag cctgaacgag caaggttttg gactgtatga
     7981 accggcgggc ggctcggcac cagatatcgc aggcaaaaac atcgccaacc cgattgcaca
     8041 aatcctgtcg ctggcactgc tgctgcgtta cagcctggat gccgatgatg cggcttccgc
     8101 cattgaacgc gccattaacc gtgcattaga agaaggcatt cgcaccgggg atttagcccg
     8161 tggcgctgcc gccgttagta ccgatgaaat gggcgatatc attgcccgct atgtggcaga
     8221 aggggtgtaa tcatggctaa aacgttatac gaaaaattat tcgacgctca cgtagtgtac
     8281 gaagccgaaa acgaaacccc gctgttatat atcgaccgcc acctggtgca tgaagtgacc
     8341 tcaccgcagg cgttcgatgg tctgcgcgcc cacggtcgcc cggtacgtca gccgggcaaa
     8401 accttcgcca ctatggatca caacgtctct acccagacca aagacattaa tgcctgtggt
     8461 gaaatggcgc gcatccagat gcaggagctg attaaaaact gcaaagaatt tggcgtcgag
     8521 ctgtatgacc tgaatcaccc gtatcagggg atcgtccacg taatggggcc ggaacagggc
     8581 gtcaccttgc cggggatgac cattgtctgc ggcgactcgc ataccgccac ccacggcgcg
     8641 tttggcgcac tggcctttgg tatcggcact tccgaagttg aacacgtact ggcaacacaa
     8701 accctgaaac agggccgtgc gaagaccatg aaaattgaag tccagggcaa agccgcgccg
     8761 ggcattaccg caaaagatat cgtgctggca attatcggta aaactggcag cgcaggcggc
     8821 accgggcatg tggtggagtt ttgcggcgaa gcaattcgtg atttaagcat ggaaggtcgt
     8881 atgaccctgt gcaatatggc gatcgaaatg ggcgcaaaag ccgctctggt tgctcctgac
     8941 gaaaccacct ttaactatgt caaaggccgt ctgcatgcgc cgaaaggcaa agatttcgac
     9001 gacgccgttg cctactggaa aaccctgcaa accgacgaag gcgcaacttt cgataccgtt
     9061 gtcactctgc aagcagaaga aatttcaccg caggtcacct ggggcaccaa tcccggccag
     9121 gtgatttctg tgaacgacaa tattcccgat ccggcttcgt ttgccgatcc ggttgaacgt
     9181 gcgtcggcag aaaaagcgct ggcctatatg gggctgaaac cgggtattcc gctgaccgaa
     9241 gtggctatcg acaaagtgtt tatcggttcc tgtaccaact ctcgcattga agatttacgc
     9301 gcggcagcgg agatcgccaa agggcgaaaa gtcgcgccag gcgtgcaggc actggtggtt
     9361 cccggctctg gcccggtaaa agcccaggcg gaagcggaag gtctggataa aatatttatt
     9421 gaagccggtt ttgaatggcg cttgcctggc tgctcaatgt gtctggcgat gaacaacgac
     9481 cgtctgaatc cgggcgaacg ttgtgcatcc accagcaacc gtaactttga aggccgccag
     9541 gggcgcggcg ggcgcacgca tctggtcagc ccggcaatgg ctgccgctgc tgctgtgacc
     9601 ggacatttcg ccgacattcg caacattaaa taaggagcac accatggcag agaaatttat
     9661 caaacacaca ggcctggtgg ttccgctgga tgccgccaat gtcgataccg atgcaatcat
     9721 cccgaaacag tttttgcaga aagtgacccg tacgggtttt ggcgcgcatc tgtttaacga
     9781 ctggcgtttt ctcgatgaaa aaggccaaca gccaaacccg gacttcgtgc tgaacttccc
     9841 gcagtatcag ggcgcttcca ttttgctggc acgagaaaac ttcggctgtg gctcctcgcg
     9901 tgagcacgcg ccctgggcat tgaccgacta cggttttaaa gtggtgattg cgccgagttt
     9961 tgctgacatc ttctacggca atagctttaa caaccagctg ctgccggtga aattaagcga
    10021 tgcagaagtg gacgaactgt ttgcgctggt gaaagccaat ccggggatcc atttcgacgt
    10081 ggatctggaa gcgcaagagg tgaaagcggg agagaaaacc tatcgcttta ccatcgatgc
    10141 cttccgccgc cactgcatga tgaatggtct ggacagcatc ggcctgaccc tgcagcacga
    10201 cgacgctatt gcgtcttatg aagcaaaaca acctgcgttt atgaattaga ccccttgccc
    10261 ggtcaaatga ccgggctttc cgctatcgtc cacgtcatca aacgtcttta acctttgcgg
    10321 ttaaaaataa tgcgacaaca gaaataatcg caattaccca gtagacagca aagtgccccc
    10381 aactttgtgc aattgctccc tgaataacac cagccagaat taccccggta gagatagtgt
    10441 tggtaaataa ggtggtagct gcccccgctc ttccaggcat taaatcctga aaccatagca
    10501 tcccaatacc cgcaacaatg ccgataaata cagcgttaaa aagttgcagc gtcatcaacg
    10561 ccatacggct atgaaagaaa atcaatccag tgtaaaacag cactcctgcc accactgcaa
    10621 tgaccatcat tcgccgctta ccataacgtt tgacatagta gccagccaga atcattgctg
    10681 gtatttccag tccagctgcc gtccccatca ggaaacccgc cagtttgtct ggcaatccta
    10741 aatcgctact gatccacaat ggcatatcaa taatgtacat agtgttgcag gtccacatta
    10801 acgtcgaggc gacaaataac atccgtacgt tactatcctg ccagtcacct tgcattgata
    10861 aagcattttc cgacggcagt tctacccgcg ccacagacag aagcataaat gcaatcaata
    10921 ccagactgag tgtgaatatc ccggcggcaa tcgaaaacat cactgtaaag ccgtaattca
    10981 acgccagcat aaaggccagc ggtggaccga ttacccatgc cagagaaagc tgcgcacgca
    11041 tcaccgagct aaacatcacc acttctcgcg ccgagttatc cgcgtattcc cgcgccagag
    11101 caaataactg tggcattgcc gtattggcca gagatgccag aagcacacca caggtgataa
    11161 gcgtcagata atgacgatta aatgcaaaca gtagcgcatt gccaatagcc atcaaacagc
    11221 aaaatataat cagttttcgc cgatcgccct gactgtcaga acgttttgcc aaccagaggc
    11281 ttacgccgat cccagcaata gcattcaccg tataaaagag tccgatccag aaaggttgcg
    11341 cgccaacctc acgactcaga aataagctca atgtaggagc ctgtagcgcc ccggccaccc
    11401 ccatcataaa agcgaccagc ataaatgccg cgtaaacacc gttcatacgg cgagccatcg
    11461 tcattatcca gatcatacgt tcccttttta gcgcggcgag aataaaaaaa accagcaggt
    11521 ataatctgct ggcgggtgat tttacaccaa tactcagtca cacatgatgc aggcaagtca
    11581 actttcagaa ttgcggtcat cccactgcat cagtccttca agcatggtta atcgttgtgg
    11641 gacgcttaac caacgcaacc agcacaactt cgctgtcgcg gtaaaatagt gctgataaaa
    11701 ctgacgcatg gggcacctcc ttgcttcatc gttaggaatt attgacttaa tatagggaaa
    11761 ataaaattgc tgtcttttgc gcaggagttc cccttttatg ccatctgctc gtctgcaaca
    11821 acagttcatc cgcctgtggc aatgctgcga gggtaaatcg caggacacaa cgctcaacga
    11881 actggcagcg ttattgagct gctcgcgtcg tcatatgcgt accctgctca acaccatgca
    11941 ggatcgcggc tggctgacgt gggaagcgga agtcgggcgc ggtaaacgct cgcgtctgac
    12001 attcctctat accgggctgg cgcttcagca acagcgggcg gaagacctgc tggagcagga
    12061 tcgtatcgac caactagtgc agttggttgg cgacaaagcg actgtgcggc aaatgctggt
    12121 ttctcatctg ggccgcagct tccgccaggg gcggcacatc ctgcgcgtgc tctactatcg
    12181 tccgttgcgt aatctgctac ctggcagcgc attgcgccgt tccgaaaccc atattgcccg
    12241 gcaaatcttc agttcgctaa cgcgcataaa tgaggaaaat ggggaactgg aagcagacat
    12301 cgcccaccac tggcagcaaa tatcaccgct tcactggcgt ttctttttgc gtccaggagt
    12361 ccattttcat catggtcgtg aactggaaat ggacgacgtg atcgcctctt taaaacgaat
    12421 caatacgctg ccgctctatt cgcatattac ggacattgtg tcgccgacgc cctggacgct
    12481 ggatatccat ctcacgcagc cggaccgctg gttgccgtta ctgctggggc aagttccggc
    12541 gatgatcctg ccgtgcgaat gggaaaccct cagtaacttt gccagccatc ctattggcac
    12601 cggtccgtat gcggtgattc gcaacagcac caatcaactg aaaattcagg cattcgatga
    12661 cttcttcggt taccgggcat taatcgacga agttaacgtc tgggttctgc cggaaattgc
    12721 cgacgagcca gccggtggtc tgatgctaaa aggtccacag ggcgagaaaa aagagattga
    12781 aagccgcctg gaggaaggtt gctactattt actgttcgac agccgcaccc atcgcggggc
    12841 gaatcagcaa gtcagggact gggtaagcta tgtgctttct ccaactaatc tggtctattt
    12901 cgctgaggaa cagtaccagc aactgtggtt cccggcttat ggactgctcc cccgttggca
    12961 ccatgcccgc accataaaga gcgaaaaacc ggctggcctg gaaagcctca ccctgacctt
    13021 ttatcaggat cacagtgagc atcgggtgat tgccgggatc atgcagcaga ttctggcaag
    13081 tcaccaggtc acgctggaaa tcaaagagat cagctacgat cagtggcatg aaggagagat
    13141 cgagagcgat atctggctta atagcgccaa ctttacgctg ccgctggatt tttcgctgtt
    13201 cgcgcacttg tgcgaggtgc cgctgctcca acactgtatt ccgatcgact ggcaagccga
    13261 cgccgcccgc tggcgcaatg gcgaaatgaa cctggcgaac tggtgccagc aactggtcgc
    13321 cagtaaagca atggtgccgc ttatccacca ctggctgatc attcagggac aacgcagtat
    13381 gcgcggcctg cgtatgaata ccctcggctg gttcgatttt aaatcagcgt ggtttgcgcc
    13441 accggatcca tgattgctgg tagataacaa aatcactaca ctaacgccgt tctcaacggg
    13501 gtgccacgca aacgcgtgcg ctgagaaaat acccgtcgaa cctgatccgg ataacgccgg
    13561 cgaagggatt tgaggctcct tctcaagtcc tttgccactc ttttttgagg tgcaaagtgt
    13621 taaaaaaatg tcttcccctg ctgttgctgt gcacagcgcc cgttttcgct aaacccgttc
    13681 tgactgttta tacctacgat tccttcgccg ccgactgggg gcctggtcca aagatcaaaa
    13741 aagcttttga agccaactgt aactgcgaac tgaaactggt ggcgctggaa gatggcgttt
    13801 cgcttctcaa ccgtctacgg atggaaggca aaaatagtaa agccgatgtg gtgctggggc
    13861 tggataacaa tctgttagac gccgccagta aaaccggact atttgccaaa agcggtgtgg
    13921 cagcggatgc cgttaacgtt cccggcggct ggaataatga cactttcgtc ccgtttgatt
    13981 acggctattt cgccttcgtc tatgacaaga acaaactgaa aaacccgccg caaagcctga
    14041 aagaattggt tgagagcgat cagaactggc gggtgattta tcaggatccg cgtaccagca
    14101 cgccggggct gggtctgctg ttgtggatgc aaaaagtcta tggcgatgac gccccacaag
    14161 cctggcaaaa actggcgaag aaaaccgtca cggtcaccaa aggctggagc gaagcctacg
    14221 gcctgttttt aaaaggtgaa agcgatctgg tactgagtta caccacctct ccggcttatc
    14281 acattctcga agagaaaaaa gataactacg ccgccgcgaa ctttagcgaa ggtcactatc
    14341 tgcaggtgga agtcgccgcc cgcaccgctg ccagcaagca gccggagctg gcgcaaaaat
    14401 tcctccagtt tatggtttct ccggcttttc agaatgcgat cccaaccggt aactggatgt
    14461 atccggtggc aaatgtcacg ctgcctgccg ggtttgaaca attgaccaaa ccggccacca
    14521 cgctggagtt cacaccagct gaagtggcgg cacaacgtca ggcatggatt agcgaatggc
    14581 aacgcgccgt cagccgttaa ttcccggctg gttaattcca ggtgtaagcg ccgccacgct
    14641 ggtggtagcg gttgcgctcg cggcgtttct cgccctgtgg tggaacgcgc cgcagggtaa
    14701 ctgggtggca gtctggcagg acagctacct gtggcatgtg gtgcgcttct ccttctggca
    14761 ggcgtttctc tcggcgctac tctctgtcgt acccgcgata ttcctcgccc gcgccctcta
    14821 tcgcaggcgc tttccgggtc ggctggcgct gttgcgtctg tgcgcaatga ccttgatcct
    14881 cccggtgcta gttgctgttt tcggcattct tagcgtctat ggtcgtcagg gctggctggc
    14941 atcgctctgc caatcgctcg gtctggagtg gaccttttcg ccctacggcc tgcaaggcat
    15001 tttgctggcg cacgtatttt ttaatctgcc gatggcgagc cgcttattac tccaggcact
    15061 ggaaaacatt cccggcgaac aacgtcagct tgccgcccag cttgggatgc gcggctggca
    15121 ttttttccgc ttcgtcgaat ggccgtggct acggcgacaa atcccgccgg ttgccgcgct
    15181 tatctttatg ctctgtttcg ccagcttcgc caccgtgcta tcgctgggcg gcggtccgca
    15241 ggcaaccact atcgagctgg ctatttatca ggcgctgagt tacgactacg atcctgcccg
    15301 cgcggcgatg ctggcgctgc tccagatggt gtgttgcctt gggctggtgc tgttgagtca
    15361 gcgactaagt aaggccattg cgcccggcac cacgctgctg caaggctggc gcgatccgga
    15421 cgatcgtctg catagccgca tttgcgacac ggtgttaata gtgctggcgc tgctgctgtt
    15481 gctgccaccg ttgctggcgg tgatcgtcga tggggtaaat cgccagttgc cggaagtgct
    15541 ggcacaaccg gtgctgtggc aggcgctgtg gacctcgttg cgtattgcgc tggcggcagg
    15601 tgtattgtgc gtagtgctga ccatgatgct gctatggagc agtcgcgaac tgcgggcgcg
    15661 gcagaaaatg ctggcgggtc aggcgctgga gatgagcggc atgttgatcc tcgccatgcc
    15721 ggggattgtg ctggctaccg gcttcttttt actgctcaac aacactatcg gcctgccaca
    15781 atctgctgac ggcattgtga ttttcaccaa tgcgttaatg gcgatccctt atgcgctgaa
    15841 agtactggaa aacccgatgc gcgatatcac cgcccgctac agtatgttgt gtcagtcgct
    15901 gggcattgaa ggctggtcgc gcttaaaagt ggtcgagctg cgcgccctga aacgtccact
    15961 ggcgcaggcg ctggcttttg cctgcgtgct gtcgattggt gattttggcg tagtggcgtt
    16021 gttcggtaac gatgatttcc gtaccctgcc gttttatctc taccagcaaa ttggctccta
    16081 tcgcagccag gacggcgcgg tcaccgcgtt aattctgcta ctactctgtt ttctgctgtt
    16141 taccgtgatt gaaaaactac cggggcgaaa tgttaaaact gactgatatc acctggcttt
    16201 atcaccattt gccgatgcgt tttagcttaa cggtagaacg cggcgagcag gtggcaatcc
    16261 tcgggccaag cggcgcgggt aaaagtaccc tgctgaattt gattgccggt tttctgacgc
    16321 cagccagcgg ttcgctgact atcgatggtg ttgatcacac cacgacgccg ccgtcacgcc
    16381 gtccggtgtc gatgctgttt caggagaaca acctgttcag ccatctgacg gtcgcgcaga
    16441 acatcgggct ggggctaaat ccggggttga aactgaacgc ggcacagcag gagaaaatgc
    16501 acgcgattgc ccgtcagatg gggattgata atttaatggc gcggttaccg ggcgagcttt
    16561 ccggcggtca gcgacagcgc gtggcgttag cgcgttgtct ggtgcgcgaa cagccgattt
    16621 tattgctcga tgaaccgttc tctgcgctcg atccggcgtt acgtcaggag atgctgacgc
    16681 tggtgagcac gagctgccag cagcaaaaaa tgacgctatt gatggtgtcg cacagtgtgg
    16741 aagatgcggc gcggatcgcc acacgctcgg tggtggtcgc cgacgggcgc atcgcctggc
    16801 agggtaagac tgaagaattg ttgagcggta aggcgagtgc ttcggcaata ttggggatta
    16861 cgggttagta tgcactgtca gatgcggcgt gaacacctta tccgacctac ggacagtgcg
    16921 ttttgtaggc ctgataagac gcggtcagcg tcgcatgagg caacccgcac aagacaatcc
    16981 cctaaacccc aaccacttta cgcaaaatat cgatatacac cggcatcaac gggtggcgaa
    17041 ttaacaccac cagcgccacc acgccgatgg cagaaatcaa cggcgtcagc cacaacaaac
    17101 ggccgcgcga caaataatga cttaaacgat cggttgcttt accgctgcgc cataaccgcc
    17161 agcacagcca gccaccaacc cacaaaaata ccgccgtcgc cagcagcaac catttaaact
    17221 cgccgctctg cattccggct ggaatatcga tcgccgcgcc cgccagaatc ccaggcagga
    17281 agtaaaacgg tggccacagc aggcagccga taatattcgg cgtaatgaat tttgccaccg
    17341 gcagatccag cattcccgcc accattggca ccagcggacg cgtcgggcca acaaagcgac
    17401 cgaccagaat ggtgaacatg ctgtgttgat gcaacgcatg ttcagtttta tcgagtaatg
    17461 ctttgttttt cttcagaaat gaccagcgat gcaacggctt tttaaaacgc cagcccagcc
    17521 agaaggagat ccagtcgccc agcagacaac cgacaatccc tgccagccaa gcgtgccaga
    17581 aacttaactc gccgctgcca atcagcgctc ccagccccgc catcagcacc gtaccgggta
    17641 gaatcaaacc gaccagcgcc agcgactcca gaaaggccac caacaccacc gccatcaatg
    17701 aatacacggt agattgggta ataaagtgtt ccagcaatgc ttgcataatg tgcctgtcaa
    17761 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
    17821 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
    17881 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
    17941 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
    18001 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
    18061 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
    18121 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacacga
    18181 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
    18241 tcaagcagat tcatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
    18301 atttgcccaa acaggtcgct gaaatacggc tggtgcgctt catccgggcg aaagaaaccc
    18361 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
    18421 taaacccact ggtgatacca ttcgcgagcc tccggatgac aaccgtagtg atgaatctct
    18481 cctggcggga acagcaaaat atcacccggt cggcagacaa attctcgtcc ctgatttttc
    18541 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
    18601 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
    18661 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
    18721 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
    18781 gtcttttact ggctcttctc gctaacccaa ccggtaaccc cgcttattaa aagcattctg
    18841 taacaaagca ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
    18901 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
    18961 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
    19021 tacccgtttt tttggatgga gtgaaacgat ggcgattgca attggcctcg attttggcag
    19081 tgattctgtg cgagctttgg cggtggactg cgccagcggt gaagagatcg ccaccagcgt
    19141 agagtggtat ccccgttggc aaaaagggca attttgtgat gccccgaata accagttccg
    19201 tcatcatccg cgtgactaca ttgagtcaat ggaagcggca ctgaaaaccg tgcttgcaga
    19261 gcttagcgtc gaacagcgcg cagctgtggt cgggattggc gttgacagta ccggctcgac
    19321 gcccgcaccg attgatgccg acggtaacgt gctggcgctg cgcccggagt ttgccgaaaa
    19381 cccgaacgcg atgttcgtat tgtggaaaga ccacactgcg gttgaagaag cggaagagat
    19441 tacccgtttg tgccacgcgc cgggcaacgt tgactactcc cgctatattg gcggtattta
    19501 ttccagcgaa tggttctggg caaaaatcct gcatgtgact cgccaggaca gcgccgtggc
    19561 gcaatctgcc gcatcgtgga ttgagctgtg cgactgggtg ccagctctgc tttccggtac
    19621 cacccgcccg caggatattc gtcgcggacg ttgcagcgcc gggcataaat ctctgtggca
    19681 cgaaagctgg ggcggcttgc cgccagccag tttctttgat gagctggacc cgatcctcaa
    19741 tcgccatttg ccttccccgc tgttcactga cacctggact gccgatattc cggtgggcac
    19801 cttatgcccg gaatgggcgc agcgtctcgg cctgcctgaa agcgtggtga tttccggcgg
    19861 cgcgtttgac tgccatatgg gcgcagttgg cgcaggcgca cagcctaacg cactggtaaa
    19921 agttatcggt acttccacct gcgacattct gattgccgac aaacagagcg ttggcgagcg
    19981 ggcagttaaa ggtatttgcg gtcaggttga tggcagcgtg gtgcctggat ttatcggtct
    20041 ggaagcaggc caatcggcgt ttggtgatat ctacgcctgg tttggtcgcg tactcggctg
    20101 gccgctggaa cagcttgccg cccagcatcc ggaactgaaa gcgcaaatca acgccagcca
    20161 gaaacaactg cttccggcgc tgaccgaagc atgggccaaa aatccgtctc tggatcacct
    20221 gccggtggtg ctcgactggt ttaacggtcg tcgcacgcca aacgctaacc aacgcctgaa
    20281 aggggtgatt accgatctta acctcgctac cgacactccg ctgctgttcg gcggtttgat
    20341 tgctgccacc gcctttggcg cacgcgcaat catggagtgc tttaccgatc aggggatcgc
    20401 cgtcaataac gtgatggcgc tgggcggcat cgcgcggaaa aaccaggtca ttatgcaggc
    20461 ctgctgcgac gtgctgaatc gcccgctgca aattgttgcc tctgaccagt gctgtgcgct
    20521 cggtgcggcg atttttgctg ccgtcgccgc gaaagtgcac gcagacatcc catcagccca
    20581 gcaaaaaatg gccagtgcgg tagagaaaac cctgcaaccg cgcagcgaac aggcacaacg
    20641 ctttgaacag ctttatcgcc gctatcagca atgggcgatg agcgccgaac aacactatct
    20701 tccaacttcc gccccggcac aggctgccca ggccgttgcg actctataag gacacgataa
    20761 tggcgatttt tgataattat gaagtgtggt ttgtcattgg cagccagcat ctgtatggcc
    20821 cggaaaccct gcgtcaggtc acccaacatg ccgagcacgt tgttaatgcg ctgaatacgg
    20881 aagcgaaact gccctacaaa ctggtgttga aaccgctggg caccacgccg gatgaaatca
    20941 ccgctatttg ccgcgacgcg aattacgacg atcgttgcgc tggtctggtg gtgtggctgc
    21001 acaccttctc cccggccaaa atgtggatca acggcctgac catgctcaac aaaccgttgc
    21061 tgcaattcca cacccagttc aacgcggcgc tgccgtggga cagtatcgat atggacttta
    21121 tgaacctgaa ccagactgca catggcggtc gcgagttcgg cttcattggc gcgcgtatgc
    21181 gtcagcaaca tgccgtcgtt accggtcact ggcaggataa acaagcccat gagcgtatcg
    21241 gctcctggat gcgtcaggcg gtctctaaac aggatacccg tcatctgaaa gtctgccgat
    21301 ttggcgataa catgcgtgaa gtggcggtca ccgatggcga taaagttgcc gcacagatca
    21361 agttcggttt ctccgtcaat acctgggcgg ttggctatct ggtgcaggtg gtgaactcca
    21421 tcagcgacgg cgatgttaac gcgctggtcg atgagtacga aagctgctac accatgacgc
    21481 ctgccacaca aatccacggc gaaaaacgac agaacgtgct ggaagcggcg cgtattgagc
    21541 tggggatgaa gcgtttcctg gaacaaggtg gcttccacgc gttcaccacc acctttgaag
    21601 atttgcacgg tctgaaacag cttccaggtc tgcccgtaca gcgtctgatg cagcagggtt
    21661 acggctttgc gggcgaaggc gactggaaaa ccgccgccct gcttcgcatc atgaaggtga
    21721 tgtcaaccgg tctgcagggc ggcacctcct ttatggagga ctacacctat cacttcgaga
    21781 aaggtaatga cctggtgctc ggctcccata tgctggaagt ctgcccgtcg attgccgtag
    21841 aagagaaacc gatcctcgac gttcagcatc tcggtattgg tggtaaggac gatcctgccc
    21901 gactgatctt caatacccaa accggtccag cgattgtcgc cagcctgatt gatctcggcg
    21961 atcgttaccg tctgctggtt aactgtatcg acacggtgaa aacaccgcac tccctgccga
    22021 aactgccggt ggcgaatgca ctgtggaaag cgcaaccgga tctgccaact gcttccgaag
    22081 cgtggatcct cgctggtggc gcgcaccata ccgtcttcag ccatgcgctg aacctcaacg
    22141 atatgcgcca gttcgccgag atgcacgaca ttgaaatcac ggtgattgat aacgacaccc
    22201 gcctgccagc gtttaaagac gcactgcgct ggaacgaagt gtattacgga tttcgtcgct
    22261 aagtagccac atcaggtatg taacgcctga tgcgacgctg acgcgtctta tctggcctac
    22321 acgctgcgat tttgtaggcc ggataagcaa agcgcatccg gcattcaacg cctgatgcga
    22381 cgctggcgcg tcttatcagg cctacgcgct gcgattttgt agaccggata agcgaagcgc
    22441 atccggcacg aaggagtcaa catgttaaaa gatctcaaac gccaggtatt agaagccaac
    22501 ctggcgctgc caaaacacaa cctggtcacg ctcacatggg gcaacgtcag cgccgttgat
    22561 cgcgagcgcg gcgtctttgt gatcaaacct tccggcgtcg attacagcgt catgaccgct
    22621 gacgatatgg tcgtggttag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc
    22681 tcctccgaca cgccaactca ccggctgctc tatcaggcat tcccctccat tggcggcatt
    22741 gtgcatacgc actcgcgcca cgccaccatc tgggcgcagg cgggtcagtc gattccagca
    22801 accggcacca cccacgccga ctatttctac ggcaccattc cctgcacccg caaaatgacc
    22861 gacgcagaaa tcaacggcga atatgagtgg gaaaccggta acgtcatcgt agaaaccttt
    22921 gaaaaacagg gtatcgatgc agcgcaaatg cccggcgttc tggtccattc ccacggcccg
    22981 tttgcatggg gcaaaaatgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaggtc
    23041 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
    23101 ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga
    23161 ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata ctcaagcctg gttttttgat
    23221 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
    23281 aagggacaga agtctccttc tggctggcga cggacaacgg gccgttgcag gttacgcttg
    23341 caccgcaaga gtccgtggcg tttattcacg ccgatcaggt tccccgcgct cagcatattt
    23401 tgcagggtga acaaggcttt cgtctgacac cgctggcgtt aaaggatttt caccgccagc
    23461 cggtgtatgg cctttactgt cgcgcccatc gccaattgat gaattacgaa aagcgtctgc
    23521 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
    23581 agcggtttat cacctcaccg gtgtgggtcg agggtgatat gcacaatggc actatcgtta
    23641 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctatagata
    23701 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggctgc gggcagcgca
    23761 tcgtttatat gctggggccg gagaatggcg acgcctcctc gcttgatttc gaactggaat
    23821 acgtcgccag ccgcccgctg ctgctggaaa aactcaacgc ctggtttgca aaccacgatc
    23881 ccgatgtgat catcggctgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg
    23941 ccgagcgtta ccgtcttccg ctgcgtcttg ggcgcgataa tagcgagctg gagtggcgcg
    24001 agcacggctt taaaaacggc gtcttttttg cccaggctaa aggtcggcta attatcgacg
    24061 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaactgtcg
    24121 ctcaggagct attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa
    24181 ttgaccgccg tttcgccgaa gataaacctg cgctggcaac ttataacctg aaagattgcg
    24241 agctggtgac gcagatcttc cacaaaactg aaatcatgcc atttttactc gaacgagcaa
    24301 cggtgaacgg cctgccggtg gatcggcacg gcggttcggt ggcagcgttt ggtcatctct
    24361 attttccgcg aatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc
    24421 acgccagccc tggcggctac gtgatggatt cacggccagg gctttatgat tcagtgctgg
    24481 tgctggacta taaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
    24541 ggctggttga aggcatggcg caacctgatc cagagcacag taccgaaggt tttctcgatg
    24601 cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc
    24661 gcgataaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
    24721 atgcctttta tggcgtgctc ggcaccaccg cctgccgctt cttcgatccg cgcctggcat
    24781 cgtcgatcac catgcgtggt catcagatca tgcggcagac caaagcattg attgaagcgc
    24841 agggctacga cattatctac ggagataccg actcgacttt tgtctggcta aaaggtgcac
    24901 attcggaaga agaggcggcg aaaatcggtc gtgcactggt gcagcacgtt aacgcctggt
    24961 gggcggaaac gctgcaaaaa caacggctga ccagcgcatt agaactggag tatgaaactc
    25021 atttctgccg ttttcttatg ccaaccattc gcggagctga taccggcagt aaaaagcgct
    25081 acgccgggct gattcaggag ggcgacaagc agcggatggt gtttaaaggg ctggaaaccg
    25141 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct
    25201 tccgcaacga gccatatcag gagtacgtcc gccaaaccat cgacaaactg atggcgggtg
    25261 aactggatgc gcggctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
    25321 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaat caaaagcgcg
    25381 gtcgcccctt gcaatatcag aatcgcggca ccattaagta cgtatggacc accaacggcc
    25441 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
    25501 tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct acacttatga
    25561 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
    25621 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    25681 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    25741 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    25801 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    25861 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    25921 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    25981 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    26041 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    26101 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    26161 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    26221 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    26281 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    26341 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    26401 aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc
    26461 tcagcacgat gcttacaacc cgtttgacac cgaacagctg gtgatttgct cgctggattt
    26521 tgcccgtcgt agcaaacagc gcttggaaca tctctgtgaa gccgaatggg acctgctggt
    26581 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    26641 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    26701 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    26761 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    26821 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    26881 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    26941 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    27001 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    27061 gccgctaccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    27121 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    27181 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    27241 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    27301 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    27361 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    27421 ggtactgctg tgctcagaaa tcggttctga aggacgtaac tttcagttcg ccagtcacat
    27481 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    27541 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    27601 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    27661 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccgga
    27721 tcaaaccgaa ggctttgacg atctgatcaa aagctgccgc gagcaacatg aagcgctgaa
    27781 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    27841 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    27901 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    27961 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    28021 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    28081 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
    28141 cagcacgatt tcactgttaa aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    28201 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    28261 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    28321 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    28381 cgccgtgcag caggatgttc acgctatcct tcaactgggt gaagcgcaga tcgagaaatc
    28441 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
    28501 gtctcgtctg gaagctctgc gtgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    28561 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggtt ggcgtctgga
    28621 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    28681 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca tattatggtg
    28741 gtcaacaagc cgagcggttt gttgtcagtg ccgggtcgtc tggaagagca caaagacagc
    28801 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    28861 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    28921 cagttccgcg agcgcgagcc gaaaaagcag tatgtggccc gcgtctgggg gcatccatcc
    28981 cccgcagaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    29041 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    29101 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcaattgcgt
    29161 gtgcatatgc tggcgctggg gcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    29221 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    29281 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    29341 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    29401 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    29461 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    29521 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    29581 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    29641 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    29701 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    29761 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    29821 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    29881 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    29941 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    30001 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    30061 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    30121 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    30181 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    30241 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    30301 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    30361 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    30421 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    30481 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    30541 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    30601 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    30661 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    30721 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    30781 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    30841 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    30901 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    30961 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    31021 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    31081 ccgcgaagaa caagttgctg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    31141 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    31201 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    31261 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    31321 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    31381 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    31441 ttggttattc tactggaacc actccggggt catggatcag gtgtggcgtt tcaacgtcga
    31501 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    31561 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    31621 caccgtttca accaagcagt tccaggtttt cagcgaacag aacaccagta gctactcggc
    31681 agagccgcag ttagacgtta attactacca gaatgatgtt ggtccgtttg atacgcgtat
    31741 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    31801 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    31861 agcgaagttt ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    31921 cacgaccaag ctggacgaat ccgttaaccg cgtaatgccg caattcaaag ttgacggcaa
    31981 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    32041 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    32101 gtctctgctg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    32161 ccgtattgcc tccgctaacc aagtgacgac cggtgtcaca tctcgcatat atgatgatgc
    32221 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    32281 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    32341 cgacacctac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    32401 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    32461 tctggtacag ctgaattacc attacgccag cccggaatat attcaggcta cgctgcctaa
    32521 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    32581 caggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    32641 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    32701 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    32761 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    32821 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    32881 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    32941 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    33001 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    33061 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    33121 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    33181 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    33241 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacactt
    33301 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    33361 gtcgcatcac catcctgcca caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    33421 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    33481 cctctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    33541 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    33601 gcggccagat ggggtggggc cgtattcagg agttgccggg gatcttcgcc caggcattaa
    33661 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    33721 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    33781 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgc gtgaaactgg
    33841 agcaaattgc tgctgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    33901 tctcgcagga tccaggttct gctaaccagg gcggtgatct cggctgggct acaccagata
    33961 ttttcgatcc agccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    34021 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    34081 aaaccgacgc tgcgcagaaa gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    34141 aagaagcagc aagctggatg caggaacaac gtgccagcgc ctacgttaaa atcctgagca
    34201 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    34261 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    34321 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    34381 ttcccccaac tcccctgcac aaccgcaaac tacgggcaca ttaacgctac ttcctgtcgc
    34441 gctacgtgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    34501 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    34561 ccccgtgcat aaaggcgtca ttaacgacgc aagcattccg tttaccggtc ataccgagtt
    34621 tttcgaagag cgttcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    34681 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    34741 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    34801 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    34861 tacggaagag atagacacca ttattccggt gctcgacgag ctgcgggcgc aggggatgaa
    34921 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    34981 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    35041 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    35101 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    35161 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    35221 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    35281 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    35341 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    35401 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    35461 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    35521 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    35581 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    35641 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    35701 atcccggtac tggaagttcc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    35761 gtcgtgcgcc tggtccctca tgcaacgatg cctcacccgg ttaaagatat tcgcgtgttg
    35821 agccgcatca ccaccgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    35881 aacctgttta gcgtcgaggt gttaaccgga atggggatcg acccggcgat gcgagcggaa
    35941 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    36001 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    36061 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    36121 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    36181 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    36241 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    36301 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    36361 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    36421 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccccg
    36481 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cggcccgggt tcgctggatg
    36541 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    36601 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    36661 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctctgc
    36721 tgcaaatcga cgaagagaaa aagctggtga tggcccacgc agggatcacg ccgcagtggg
    36781 atctgcagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctgtcg agtgactcct
    36841 atcccttctt tcttgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    36901 gggggctggg aagactgcgt tttatcacca acgcctttac ccgtatgcgt ttttgcttcc
    36961 cgaacggtca actggatatg tacagcaaag aatcgccgga agaagcccct gccccactga
    37021 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    37081 gggcatcgct ggaaggcaaa ggtacgccgg aaggtattta cgcgctggat accggctgct
    37141 gctggggtgg gtcattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    37201 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatacaggaa
    37261 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaactt
    37321 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatcg gcgtcgtgga
    37381 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    37441 ccacttctgc gtcgatatgc gtcagataca gtttttgcgc ttttggcagg aactgctcat
    37501 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcaatggctt
    37561 catccaccga tttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    37621 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    37681 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    37741 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    37801 agatttcccg ataaaaaaat tgtcgccact atacgtaaag cgtaaaccgt cgtcgactgg
    37861 tgcgaggatg atgttgagga aaattttata ttctgctggc gagtccacgc tctccccctg
    37921 gactcgccgc attacaatga aacaaaaaca aacagttagc tgtaaagtgt gatttacgtc
    37981 actctttatt aagatgaggg tttcgtttcc ggttcatccg ccatgttgcc ggtatgttta
    38041 ccttcttctg ttccctgcca gccatgtcgt tgaattaatg acagatgttc gcggtcctcg
    38101 gtgatgatct cacttaacat cgcgctggtg cgtttataga ccgccgcgcg ggctttggtg
    38161 tcattctcaa ccattgccat ctcttccacc atctgaatat taaagcggcg gaacacatcg
    38221 gcacgttctc gcgcttcata cggccccaga cctaaacttt ccagcgccag acgcccggtt
    38281 ttcagcgcac cttcaaaggt ttcacgctcc ggcttttcaa cgcctgcctg acgcaaacga
    38341 atgtagtgat cgacatcgcg ggcgcgggca ataatctgca aatgcgggaa atgttctttc
    38401 accatctccg tcagttgcag gttggtttgc ggatcgtcaa tagcgttaat cagcacttcc
    38461 gctttcgccg ctccggcaga ttccagtaaa tccatccgcg tggcatcgcc ataaaacact
    38521 ttcataccaa atttacgcag cgtttcgata tggtccggat cgtgatcgag caccaccatt
    38581 ttcaccccgc tggagagcag taaacgtccg gtaatctgtc caaaacgacc gaatccggcg
    38641 ataatcacgc gcggctgttc ttcatcgatc tcatcggctt cacgcgcctc ctccgtagaa
    38701 gattgctcaa ggcgattgag gatcaccagc agaatcggcg ttgctgccat cgacagcgcc
    38761 accgccaggg tcagcgattt cacccactcc ggctccagca cattcgccat ctgcgccgca
    38821 ccaaatacca caaaggcaaa ctcactgccc tgccctaaca acaccgcaaa ccagcgacgc
    38881 tgtttatttg gcacctgcaa cggtcgggca atcagccaca gcatggcgat tttgatgatg
    38941 aggaaaccga gcagcaaaat gacaatgcgc aatgggtttt caatcagcgt gccaaagtct
    39001 atcgacatgc caacaccgat gaaaaacagc cccaacagca aacctttaaa cggttcgata
    39061 tcgctttcca acgcatgacg gtattccgag cttgccagca gtacgcccgc cagaaacgcg
    39121 cccatcgcca tcgacaagcc gacctcttcc agcagcaaac caaagccaaa cacaaggaat
    39181 aacgccacgg cactaaacac ttcccgcaag ccagagcggg caacaaaacg cagcgccgga
    39241 cgcgtaacat agcgccccag caataccacc agtaccagcg cacccgccac ttttaacgcc
    39301 gagagagcaa atgcgcccat cgtcgtcgag gcactgctcg ccgccagtag cgggatcatc
    39361 gccaccagcg ggattgccgc gatatcctgg aacagcagca ctgcaaaggc actgcggccc
    39421 atttgcgtca ccatcagatt gcgttcattc atcgcctgca tggcaatcgc cgttgatgag
    39481 agcgccagcg tcataccgat caattccgcg acctgccagc gcagcccaag taacatacag
    39541 aacagcccca gcaggccacc gcaaatcacc atctgcaatg cgccaccgcc gaacactgcc
    39601 gcacgcagct tccacagcct ttgtggatcg agttcgaggc cgataataaa cagcatcagc
    39661 accaccccaa tctcggcaaa gtgcagaata gattcggcat cggtcaccag tcgcagcccc
    39721 cacgggccaa taatgcagcc ggcgatcagg tagccaagta ccgatcccag accaagacgt
    39781 accgcaatgg gtacaatcag cgccgccgat ccgaggtaga tcagcgcctg aaccagcgta
    39841 tgactatcca tgatgggcct cctgccattc cagcagacgt tgcttatagt ggcgcgcctg
    39901 cccttcgagg gtttcgtcgt cacaaataaa ggtgcagtgc atggcaaacg gtggcagcca
    39961 gttcagcccg cagtagattg ccgtcgcctg tagcggctgc gacagcacat caaagcccgg
    40021 atgcgcacca atttcaaaat ggctttcccc gccgccggtc gtcaccgccc acagcaaatg
    40081 tttgccatgc agcgccgtgc cgccgtgacc gtaagcccag ccgtgcgaca aaactttatc
    40141 gatccaaagt ttaagaagcg gaggaatgct gtaccactgc atcgggtgct gccagacgat
    40201 cagatcggcg cgagagagcg cctcctgctc ggcggcaata tcgatattga agtcaggata
    40261 gagttgataa agagagcgaa tttcgacgcc ttccagcgtc cttgcctgtt caagcatccg
    40321 tttattcgca tgggaatgat gcggatacgg atgcgcataa atgatgagga tcatagttat
    40381 tagcccgcgt gtttacgcct ttgttttccc acaaagtgta gtcatatatt cacgcggctt
    40441 atagtcaata atattgattc agaaaagaga gaaatctgaa caaataatcc cattctctgc
    40501 ccgcggatcg tcatattgct ggtctgcgcc agtgacattc ctcgagtctc cggggcaaac
    40561 gctacggaaa tcaacaagcc aaacagcgag atacccgccc ccattagcat cgtgttactg
    40621 ataccgtaat tattgataaa tatcggcagt gcccaggtcg aaacaatggt gccaatacga
    40681 cttaaggaca taatcacgcc cacggcagag gcgcggatat ccgtcgggaa gagttcatta
    40741 ggatagagcc actgcaaatt acccggcccg ccagagaaaa aggcatacac cgcaaaggcc
    40801 atcactacca gccagatccc catatccggg attagcccca aaaccgccaa cgccagcgtc
    40861 atcatggcaa agctgccaat caacaatgga cgccgtccgg cagtgtttag ccacagcatc
    40921 ggcggaatac agccgagcat aaagaacagg ctaatcacca cattccctag tgccgcgttt
    40981 ttgccaaccc ccaatcccaa cgaaccaacg atttgcgggc caaaggtgta aatggcgaac
    41041 attgggatca cctggcaggt ccagatggcg gcaacaaaca gaacaaaagg aaaatggcgg
    41101 cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct cttcatcgaa agccaccggt
    41161 tcgccaaaca gttttatcat catctcttcg cactctttta ctcgcccttt gcgtaataac
    41221 cagcggggag attctggcag ttcgaatcga ccaatcaaaa tcaacaaaca agggatcgcc
    41281 gcgctaccca gcatccagcg ccaaccacct tccacatcat aaagccagta gccgaccaga
    41341 tcggcgcagg tcgcgccgac ataccacatc gcggcaataa agctgatgga aaacgcccgc
    41401 tgacgggtac tggagaactc ggtgatcatc gaagtggcga tgggataatc tgcaccgatg
    41461 acaatgccga tgagtacccg catcaccaac agttcgacgg gggatgaaac acacatcgtc
    41521 gccaccgata tcacgccgat ggcgatgata tcaatgagga acattttgcg ccgtccgact
    41581 ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca gcccggcgag cgttcccgcg
    41641 cccagcaagc caatccagtc agcgtccagt ttcagcgccg gagtcagttg ctccagcgcc
    41701 acgccaatca ttaccagtac ataaccatcc agaaacggac caccgcttcc ccacagcaaa
    41761 atgcggcggt gaatagagga gaatttgaga tcgtcaaagt ttctggacgg ttgcatgggt
    41821 tattcctgtt tttttgcaat gagagtcagt acggcgttac gtatcaaacc aacatcagcc
    41881 gtaacggaac tccacaccaa aggtgccgcg cgggtattcc cactgttcca gcgccgaccc
    41941 cagccccaga atgcgacagg tgccgcactc cagacatccg gcgtaatcga agcgcacact
    42001 gccgtcatcc tgcttcttgt acagacctgc ggggcacgct ttcaccagca gctccagcac
    42061 ctgtttatca gcatcggcct tcacaacaat gtgcggatgc tcttcatcga cattgaattt
    42121 attgacgccc agtttgacgt ccacattgac gggagaagtc ataaaacggt cactcctttc
    42181 atgccatcct tgatcagatt gatgaagccc actttcttgc cgtggcggag gattttcttg
    42241 cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt cacgcgccac gcccaccgcc
    42301 agttccgggt tgccgctaaa catgcgtggg ttatcaagga acgccggtag tttctggtac
    42361 atgcgcatat cgcgcagcgg gccactctcc agatgctgac gatattccgc gagtttttgc
    42421 ttactgaaat cgtcgctttt catcgctgaa agcacggttt ttgctgcagc ttccccggcg
    42481 gcaatcgcca gatccatacc gcgaatggta aaaccgaggt tcatacacat tccggcggca
    42541 tcaccggcaa tcaatacgcc gtcaccgacc agctccggca gcatgttgat gcctgcttcc
    42601 ggcactacgt gagcggaata ttccaccagc ttgccgcccg cgatcagcgg tgcaacggcc
    42661 ggatgctgtt tgaaatcttc cagcatttgc ggcaccgatt ttttcgcgtc atgcagatga
    42721 tgcagaccac aaaccagccc cagcgacagg gtattttcat tggtataaag gaagccgccg
    42781 cccatcaggc catcggtggg tgatcccgca aacagacaag ccgcgccctg attaccctgc
    42841 aactgaaaac ggtcttcaat taccgacttc ggtaactcga tcagttcctt cacgccaacc
    42901 gccacatccg tcggtttaac gcgttttgcc atccccaact tttcggcaag gatggagttc
    42961 accccatcag caaggagcac cgttttcgct tcaatcacat caccatcggc ttctacaccg
    43021 acgactttgc catcgcgctg tacgaggtta tcgacgcgga tcccggtaat taactgcgcg
    43081 cccgcttctt cggcctgctc catcaaccag gcatcaaatt tactgcgcaa aacggagtaa
    43141 gaacgctggg atggcgaggt ttcgtcaccg ttgcagtagt ccatagtcat cgctgacttt
    43201 tccgtcataa acgcgagttt ttcatgggtg atcaggcgtt ctacgggggc ggaatcggcg
    43261 aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt aacgttcttg
    43321 gcacctgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg ggcgagcacc
    43381 agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa gatatcttcg
    43441 gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga gtggatcaac
    43501 gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca atgccgtaat
    43561 ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg gtttgcgacg
    43621 cgttagcgcc aaccatgtgc tggatctacc cggagatccc caccgccagg tacagttcag
    43681 gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat ttttcgtttt
    43741 ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc tgttctgcca
    43801 gcgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga cgggctttgt
    43861 cgagatcgac gctgttgctc tggcgcgcct gggttgccgt gcgggtaatc gccacagccg
    43921 gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc tgagccgcat
    43981 cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt tcttcgccaa
    44041 tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt acgctgacgg
    44101 tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc gccagtaatt
    44161 tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg cgaatagtgt
    44221 cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg tttaatttcc
    44281 agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg aggacaaagg
    44341 cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga cgagaggggg
    44401 tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta attaaatgac
    44461 tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt caatcacgat
    44521 gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg cgtccacggc
    44581 gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg cgccgagaat
    44641 ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa caaccgcagg
    44701 cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg tgagggtatc
    44761 tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga ggatttcgcc
    44821 caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac agaggatcag
    44881 atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt gcggcagtgc
    44941 ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg atagcacatc
    45001 tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg tcacctgcgc
    45061 ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat cgtattggct
    45121 tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga ccgcaatatc
    45181 ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc cagaaatcat
    45241 gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct ttaatttgga
    45301 aataaaactt taatcaccaa tattgaaagt cagatgctat ttttttaccc acaaaagaat
    45361 ataacaaact gattaataac caaaattaac gaaaaacgcc ctctgttcac tcgaaacaaa
    45421 aatgtgatac caatcacaga atacagctta ttgaataccc attatgagtt agccattaac
    45481 gcgtccacga tgttaataat aattatatta aatgttaaca aaaataaaac aaacgggaac
    45541 gcaaaataaa tattcgtttt cacagtggaa ttaactcatg aagaatgaaa agagaaaaac
    45601 gggaatagaa ccgaaggttt tctttccgcc attaataatc gtcggcatac tttgttggct
    45661 tacagtcaga gatctggatg cggcgaatgt cgttattaat gctgtattca gttacgtcac
    45721 caatgtatgg ggatgggcat ttgaatggta tatggtggtg atgcttttcg gttggttctg
    45781 gctggtgttt ggcccgtatg ccaaaaagcg tttaggtaac gaaccgccag aatttagcac
    45841 cgccagttgg atctttatga tgttcgcctc ctgtacgtct gctgccgtac tgttctgggg
    45901 atcgattgag atctactact acatctccac cccgccgttt ggcttagaac cgaactcgac
    45961 aggagcgaaa gagttggggc tggcttacag cttgttccac tggggacctc tgccgtgggc
    46021 cacttacagc ttcctttcag tcgccttcgc ttacttcttc tttgtccgca aaatggaagt
    46081 gattcgcccc agctcgacac tggtgccgct ggcaggtgaa aaacacgcca aagggttgtt
    46141 cggtaccatc gtcgacaact tctatctcgt cgccttgatc ttcgcgatgg gtaccagtct
    46201 gggccttgcc acgccgctgg tgaccgagtg tatgcaatgg ttgtttggca ttccgcatac
    46261 cctgcaactg gacgctatca tcattacctg ctggattatc ctcaacgcca tttgcgtcgc
    46321 ttgcggtctg caaaaagggg tacgtatcgc cagtgacgtg cgtagttacc tgagcttcct
    46381 gatgctgggt tgggtgttca tcgtcagcgg tgccagcttc atcatgaact acttcaccga
    46441 ttcggtgggg atgttgctga tgtatctgcc gcgcatgttg ttctataccg atcccatcgc
    46501 taaaggcggc ttcccgcagg gctggaccgt gttctactgg gcatggtggg tgatttacgc
    46561 catccagatg agtatcttcc tcgcccgcat ctcccgtggt cgtactgtgc gtgaactgtg
    46621 cttcggcatg gtgatggggc tgacagcatc cacctggatc ctgtggaccg tgctcggtag
    46681 taacactctg ctgttgatgg ataaaaacat catcaacatt ccaaatctga tcgaacagta
    46741 cggtgtggcg cgcgccatca tcgaaacctg ggccgctctg ccactcagca ccgccaccat
    46801 gtggggcttc ttcatcctct gctttattgc caccgttacg ctggttaacg cctgctctta
    46861 taccctggcg atgtctactt gccgcgaagt acgcgatggt gaagaaccac cgctgctggt
    46921 gcgtatcggt tggtcaattc tggttggcat tatcggtatt gttctgctgg cgctcggcgg
    46981 cctgaaaccg attcaaaccg ccattatcgc cggaggatgc ccgctgttct tcgtcaacat
    47041 tatggtgacg ctctccttta ttaaagacgc gaaacagaac tggaaagatt aattaacccc
    47101 caaaatatca agaggttgaa agatggattt taatttaaat gatgagcagg aactgtttgt
    47161 cgccggtatc cgcgaactga tggccagcga aaactgggag gcctattttg ccgagtgcga
    47221 ccgtgacagc gtctacccgg aacgttttgt caaagcactg gcggatatgg gtatcgacag
    47281 tctgctgatc cctgaagagc acggtggtct ggacgcgggg tttgttactc tcgccgccgt
    47341 gtggatggag ctgggacgtc tgggggcacc aacctatgta ctgtaccagc tgccgggcgg
    47401 gttcaatacc ttcctgcgcg aaggcacaca agagcagatc gacaagatta tggctttccg
    47461 cggcaccggt aagcagatgt ggaactcagc gattaccgaa ccgggcgcgg gctccgacgt
    47521 gggtagcctg aaaacgactt atacccgtag aaatggtaag atttatctta atggtagtaa
    47581 gtgttttatt accagtagcg cctacacccc gtacatcgtg gtgatggcgc gcgacggggc
    47641 ttctccggac aaacctgtct acaccgaatg gtttgttgat atgagcaaac cgggcatcaa
    47701 agtgaccaaa cttgagaagc tcggtctgcg tatggatagc tgctgtgaaa tcacctttga
    47761 cgatgtggaa ctggacgaga aagacatgtt cggtcgggaa ggtaacggct ttaaccgcgt
    47821 caaagaagag ttcgaccatg aacgtttcct ggtagccctg accaactacg gtacggcgat
    47881 gtgcgccttt gaagatgctg cgcgctacgc caaccagcgc gtgcagtttg gcgaggctat
    47941 tggtcgtttc cagttgattc aggaaaaatt cgcccacatg gcgatcaaat taaactccat
    48001 gaaaaacatg ctgtatgaag cagcgtggaa agcagacaac ggcaccatca cctctggcga
    48061 tgcagcgatg tgcaaatact tctgcgccaa tgcggcattt gaagtggtgg atagcgcaat
    48121 gcaggtgctg ggcggtgtcg ggattgcggg caaccaccgc atcagccgct tctggcgtga
    48181 cctgcgtgta gaccgcgttt ccggaggatc tgacgaaatg cagatcctga cgctgggtcg
    48241 tgcggtgctg aagcaatacc gctaagtccg tgcgctgcct gatgcgacgc tgacgcgtct
    48301 tatcaggcct acgggagcac gaatgtaagc cggataaggc gtttacgccg catccggcag
    48361 tcatgcgcta cataaatttt tcaggagcta ataatggatc atctacccat gccgaaattc
    48421 ggaccgctgg ccggattgcg cgttgtcttc tccggtatcg aaatcgccgg accgtttgcc
    48481 ggacaaatgt tcgcagaatg gggcgcggaa gttatttgga tcgagaacgt cgcctgggcc
    48541 gacaccattc gcgttcaacc gaactacccg cagctctccc gccgcaattt gcacgcgctg
    48601 tcgttaaata ttttcaaaga tgaaggccgc gaagcgtttc tgaaattaat ggaaaccacc
    48661 gatatcttca tcgaagccag taaaggtccg gcctttgccc gtcgtggcat taccgatgaa
    48721 gtactgtggc agcacaaccc gaaactggtt atcgctcacc tgtccggttt tggtcagtac
    48781 ggcaccgagg agtacaccaa tcttccggcc tataacacca tcgcccaggc cttcagtggt
    48841 tacctgattc agaacggtga tgttgaccag ccaatgcctg ccttcccgta taccgccgat
    48901 tacttttctg gcctgaccgc caccacggcg gcgctggcag cactgcataa agtgcgtgaa
    48961 accggtaaag gcgaaagtat cgacatcgcc atgtatgaag tgatgctgcg tatgggccag
    49021 tacttcatga tggattactt caacggcggc gaaatgtgcc cgcgcatgag caaaggtaaa
    49081 gatccctact acgccggttg cggtctgtat aaatgtgccg acggctacat cgtgatggaa
    49141 ctggtgggca ttacccaaat tgaagagtgc tttaaagata ttggcctcgc acatctactc
    49201 ggtacgccgg aaatcccgga aggcactcag cttatccacc gtatcgaatg cccttacggc
    49261 ccactggtag aagaaaaact cgatgcctgg ctggcggcac ataccatcgc agaagttaaa
    49321 gaacgctttg ccgaactgaa tatcgcctgt gccaaagtac tgaccgtacc ggaactggaa
    49381 agcaatccac agtatgttgc ccgtgaatca atcactcagt ggcaaacgat ggatggtcgc
    49441 acctgcaaag ggccgaacat catgccgaaa ttcaaaaata accccggaca aatctggcgc
    49501 ggaatgccct cacatggcat ggacacggct gccattttga aaaatatcgg ctacagcgaa
    49561 aacgacattc aggagttggt cagcaaaggt ctggccaaag ttgaggacta aacgcgtggc
    49621 gtcagtcggc ttgcctgact agcgcccgcc caataacaaa cgaacaaaaa tggatagagg
    49681 tgcaatggat atcattggcg gacaacatct acgtcaaatg tgggacgatc tggcggacgt
    49741 ttacggtcat aaaacggcgc tgatttgtga atccagcggc ggagtcgtta accggtatag
    49801 ttatcttgag ttaaatcagg agattaaccg cacggcaaac ctgttttata cgctggggat
    49861 tcgcaaaggc aacaaggttg cactacatct cgacaactgc ccggaattta tcttttgctg
    49921 gttcgggctg gcaaaaattg gcgcgattat ggtgccgatt aacgcccgcc tgttacgcga
    49981 ggaaagcgag tggatcttgc aaaatagcca ggcgtgcctg ctggtgacca gtgcgcaatt
    50041 ttatcctatg tatcaacaga ttcagcagga agatgccact caattgcggc acatttgcct
    50101 gacagatgtg gcacttcccg ctgatgatgg agtgagttca tttactcaac tgaaaaatca
    50161 acaacctgcc accttgtgct atgcaccgcc gctatcgact gacgatacgg cggaaattct
    50221 cttcacctcc ggcaccacct cccgaccgaa aggtgtggtg attacccatt acaacctgcg
    50281 cttcgctgga tattactccg cctggcagtg tgcactgcgt gacgatgacg tctacatgac
    50341 ggtaatgcct gcgtttcata tcgattgcca gtgtactgcg gcgatggcgg cgttttctgc
    50401 cggggccacc tttgtgctgg tcgagaaata cagcgcccgc gccttctggg gacaggcgca
    50461 gaagtaccgc gccaccatta ccgaatgtat tccgatgatg atccgtacgt tgatggtgca
    50521 gccgccttca gcgaacgatc ggcaacaccg cctgcgggaa gtgatgtttt atctcaactt
    50581 gtcggagcag gaaaaagacg cattttgtga acgcttcggc gttcgcttgc tgacgtctta
    50641 tgggatgacg gaaaccattg tgggcattat cggcgatcgc cctagcgata aacgacgctg
    50701 gccgtcgatt ggtcgggcgg ggttttgcta cgaagcggag atccgcgacg atcacaatcg
    50761 cccgctcccg gcaggtgaga tcggtgaaat ctgtattaaa ggcgtaccag ggaaaaccat
    50821 cttcaaagag tattttctca acccgaaagc cactgcgaaa gtgctggaag ccgacggctg
    50881 gctacatacc ggcgataccg gataccgcga cgaagaaggc tttttttatt tcgtcgatcg
    50941 ccgctgcaat atgatcaaac gcggtggcga gaatgtctcc tgcgtggagc tggaaaatat
    51001 catcgccacc catccgaaaa ttcaggacat cgtggttgtg ggtattaaag attcgattcg
    51061 cgatgaagcc atcaaagcat ttgtggtgct gaatgaaggt gaaacattga gcgaagagga
    51121 attttttcgc ttctgcgaac aaaatatggc gaaatttaaa gtgccctctt atctggagat
    51181 cagaaaagat ctgccacgta attgctcggg gaaaataatt agaaagaatc tgaaataaat
    51241 gaaacgtcag ggaactaccc tgccagctaa taatcacgcc ctaaaacaat atgcattttt
    51301 tgcagggatg ttgtcgtccc taaaaaagca aaaatggaga aaaggaatga gtgaatcatt
    51361 acatctgacc cgcaatggat caattctgga aattaccctt gatcgcccaa aagcgaatgc
    51421 tattgatgca aaaaccagct ttgaaatggg cgaagtattt ctaaatttcc gtgacgatcc
    51481 gcaattacgt gtcgccatta ttaccggtgc cggagagaaa ttcttttccg cgggctggga
    51541 tttaaaagcg gcagcagaag gcgaagcacc ggatgctgac tttggcccgg gtggttttgc
    51601 gggattaacc gaaattttca atctcgacaa accagttatc gcagctgtga acggctacgc
    51661 ctttggcggc ggtttcgaac tggcgctggc ggcagatttt attgtttgtg ccgataacgc
    51721 cagcttcgcc ctgccggaag ccaaactggg catcgttcct gacagcggcg gtgtgctgcg
    51781 tctgccgaag atcctgccgc ctaccatcgt caatgaaatg gtgatgaccg gcagacgaat
    51841 gggcgcagaa gaggcgctgc gttgggggat agtcaaccgc gtggttagcc aggcggaact
    51901 gatggataac gcccgcgaac tggctcagca gctggttaac agcgccccgc tggcgattgc
    51961 ggcgctgaaa gagatctacc gcaccaccag cgaaatgccg gtagaagaag cgtatcgcta
    52021 tattcgcagc ggcgtgttga aacactatcc atcggttctg cattcggaag atgccattga
    52081 agggccgctg gcgtttgccg agaagcgcga tccggtgtgg aaaggacgtt aacaaccgtg
    52141 agctattacg cctttgaggg gttaattccg gtggttcacc cgacggcgtt tgtccatccc
    52201 agtgccgtct tgattggcga tgtgattgtg ggagccggtg tctacatcgg cccactcgcc
    52261 tcactgcgtg gtgactacgg gcggttgatc gtgcaagcgg gagccaatat tcaggatggc
    52321 tgcattatgc atggctactg cgacactgac accatcgttg gggaaaacgg ccatatcggg
    52381 catggagcga tcctgcatgg ttgtgtgatt ggtcgcgatg cattggtcgg gatgaacagc
    52441 gtaattatgg atggcgcggt cattggcgaa gagagcattg ttgccgcaat gagctttgtc
    52501 aaagcaggct ttagtggcga gaaacgccag ttgttgatgg gtacgccagc ccgcgctgta
    52561 cgcagtgtta gtgacgacga gttacactgg aaacggttga ataccaaaga gtatcaggat
    52621 cttgtcgggc gctgtcatgc atcgttacat gaaacgcagc cactgaggca aatggaggaa
    52681 aatcgccccc gtttgcaggg gacgacggat gtgacgccta aacggtgatc ttgccggatg
    52741 cgacctggtt gctggataag atgctacaac atcgtatctg gcaagcagga cgcccggtat
    52801 caacgagata aaattaacga cgcatactct ttgacaacat catctgccac ttctgctcgc
    52861 ggttaagttc cgtgagcgga acggcaggaa tacgcggtgt ctttcttacg ccgactaatt
    52921 tttcccgact gttattgcgc aggcgcgcgt aaatttgctc atcgatatcg accactttaa
    52981 ccagtcgctg acactggcat ccccgccctt ccagcttatt agggatcatc ttaacttcgc
    53041 agcttatttc tgtgacttcc gacagaatat aagtcagggt attaaccgcc ttgctgtgtt
    53101 caatatcgaa atgaatagag atctctcttg ctatcaccca ccgattttca gccatcatcc
    53161 attcggcgat taacaagtag agtggttttt caacatatcc ttcacacata cgaactccag
    53221 tttcacaaat taaaagtcta gctatgactc gttttgaagc aattaaacaa ggccatatta
    53281 aaattgtgga tatatcgata gtatgcaact tcaccgtcga taagtgtgag ctaaatcctg
    53341 cttatgtcat caaaaatatc gattccccaa aagatttact taatgggcaa aaaaaaacgg
    53401 tcctcatcag agaaccgtat tagttattcg atcaaattag cggatgaaaa atatccgcca
    53461 cgacacgcta ttatttgatc tgtgcgtgca tttcctgcac cgaaattact ttttcagtcg
    53521 catcggcatt cagcgccatc gcggtagcga aaccaccgtt cagggtggtg tcataatgca
    53581 ctttatattg cagcgcactg cgacggatca cgcgggagtc ttcaatcgca cgacggcctg
    53641 aggtggtgtt gatgatgtag gtatattcgc cattcttgat acggtcctga atgtgcggac
    53701 ggccttcatg caccttgttt accagacgcg gattgatacc cgcttcgccc agcacaatcg
    53761 ccgtgccgtg ggtcgcatcc agctcgaagc cctgtttcag cagttttgcc gccaggtcca
    53821 ccacgcgttc tttatcgcct tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg
    53881 tggagttgct gcccagctgc gctttggcga acgcttcagc gaaggtgcgg cccacgccca
    53941 tgacttcccc ggtagagcgc atttctggcc ctaacagcgg gtcaacgcct gggaatttat
    54001 tgaacggcag caccacttct ttcaccgagt agtacggcgg aataacttct ttggttacgc
    54061 cctgctcagc cagcgatttg ccagccatca cacgcgccgc cacttttgcc agcggtacgc
    54121 cggtggcttt ggagacgaac ggaacagtac gcgccgcacg cgggtttact tcaatcaggt
    54181 aaacttcgtt gtttttcacc gcaaactgca cgttcatcag accgcgcacc tgcaattcga
    54241 aggccagttt ctgcacctgc tggcgcatca catcctgaat ttcctgactt aaggtgtacg
    54301 ctggcagcga acatgcggag tcaccggagt gcacacccgc ctgctcgatg tgctccatga
    54361 tgccgccaat cagcaccatt tcgccgtcgc agatggcatc cacgtcaact tctactgcgt
    54421 catcaaggaa atgatccagc aacactggcg catcgttaga cacgctaacc gccgtctgga
    54481 agtagcgacg caggtcagct tcgtcataga cgatttccat cgcccgaccg ccgagaacgt
    54541 aagacggacg caccaccagc gggtagccaa tctcttttgc cttctcaact gccatttcaa
    54601 tagcagtaac ggtggcgttc gccggttgtt tcagtttcag acggtcaacc gcatgctgga
    54661 agcgttcacg gtcttccgca cggtcgatag catccgggct ggtgccgata accggtacgc
    54721 cagcggcttc cagtgcacgc gccagtttca gcggggtctg accgccgtac tgaacgataa
    54781 cgcctttcgg cttctcaata cgcacgattt ccagcacatc ttccagagtt accggctcga
    54841 agtagaggcg atcggaagtg tcgtagtcgg tagagacggt ttccgggtta cagttaacca
    54901 taatggtttc gtaaccgtct tcacgcagcg ccagcgaggc gtgtacgcag cagtagtcga
    54961 attcgatacc ctgaccgata cggtttggac cgccgccaag caccatgatt ttttcacggt
    55021 cggtagacgg attcgcttcg cactcttctt cataagtgga gtacatgtaa gcggtgtcgg
    55081 tggcaaactc tgccgcacag gtatccacgc gcttgtagac cgggtgcagg tcatattggt
    55141 cacgcagctt acggatttcc gcttcgcgta cgcccgccag ttttgccaag cgcgcatcgg
    55201 caaagccttt gcgtttcagc tggcgcagga attcagcgtt caggccagtg atgcccactt
    55261 ccgccacttt ctcttccaga cgcaccagtt cttcaatctg taccaggaac cagcggtcaa
    55321 tgttagtcag gttgaagacg ccgtccacag acaggcccgc gcggaaagca tcggcgatgt
    55381 accagatacg ctctgcgcct gcgtctttca gttcgcgacg gattttggtt aacgcttccg
    55441 ggtcatccag gctcactttc gggtcgaagc cagttgcgcc cacttccagg ccgcgcagcg
    55501 ctttttgcag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca accgatttca
    55561 tctgagtggt cagacggtcg ttagcaccgg cgaatttttc gaagttgaag cgaggaattt
    55621 tagtaaccac atagtcgatg gacggctcga aggaggccgg agtacgtccg ccagtgatgt
    55681 cgttcatcag ttcgtctagg gtgtaaccca ccgccagttt cgccgccact ttagcaatcg
    55741 ggaaaccggt cgctttcgac gccagcgccg aagaacggga cacgcgtggg ttcatttcga
    55801 taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacattggaa ccaccggttt
    55861 caacgccgat ttcacgcagc accgccatcg aggcgttacg catgatttga tattctttgt
    55921 cggtcagcgt ttgggctggc gcgacagtga tggagtcacc ggtgtggatg cccatcgcat
    55981 caaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcacgc accacttcca
    56041 tctcgtactc tttccagccg atcagcgact catcaatcag caactctttg gtcggagaga
    56101 gatccagacc gcgggcgcaa atttcttcaa actcttcacg gttataagcg ataccgccgc
    56161 cgctaccgcc catggtaaag gatgggcgaa taatgcacgg gaagcccacg tcagcggcaa
    56221 cagccagcgc ttcttccatc gtgtgtgcga taccggaacg cgcggtttcc agaccaattt
    56281 tcttcatcgc tacgtcgaaa cgacggcggt cttctgcttt atcaatcgca tcggcagtgg
    56341 caccaatcat ggtgacaccg aactcttcca acacgccctg acgttccagc tccagcgcgc
    56401 agttcagcgc cgtctgaccg cccatggttg gcagcaccgc gtccgggcgc tctttttcaa
    56461 taatcttgcg taccacttcc cagtgaatcg gctcgatgta ggtcgcatcg gccatttccg
    56521 ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa ccctcttcgc
    56581 gcagggcttt acatgcttgc gcgccagagt agtcaaactc acacgcctga ccgataacaa
    56641 tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc atggctcttt
    56701 tactcctgat tacttagcgg ttttacggta ctgctcaatt aacttgataa agtggtcgaa
    56761 caacggcgcg gcgtcgtgtg gaccagggct ggcttcaggg tgaccctgga agctgaacgc
    56821 cggcttatcg atgcgatgaa tgccctgtaa cgtaccgtcg aacagggatt tatgcgtgac
    56881 acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat
    56941 catcaccacg tttttctcca catctttaac cggatggttg ccgccgtggt gaccaaattt
    57001 cattttgaca gtcttcgcac cgctcgccag cgccagcagc tgatgaccga gacagatgcc
    57061 gaataccgga atatcggttt cgaggaactt ctggatggcg gtaatggcgt aatcgcacgg
    57121 ggccgggtcg ccaggaccgt tggagaggaa gatgccgtct ggattcattt tcagcacatc
    57181 ttccgcagaa gtttgcgccg gaacgatggt caggcgacag cctctatcca ccagcatccg
    57241 caggatgttg cgcttggcac caaaatcata agccacgacg tggaacggca gctcgtcttc
    57301 ttttttcgct tctggcaggc cgccggtcaa cgtccagctc ccttgtgtcc agctataggc
    57361 ttctgcggtg gtcacttctt ttgccagatc catgccgttc agacctggga acgcgcgggc
    57421 tttttctaac gccagcgccg catccgggtt atcgcctgcg ataatgcagc cattctgtgc
    57481 gcctttctcg cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa tcgccacgat
    57541 gttatggcgc ttcaggtaag aagagaggtc ttcggtatta cggaagttgc tggcaatcag
    57601 cggcaggtcg cgaatcacca gaccttgtgc atgtacctga gaagattctt catcggcgtc
    57661 attagtgccg acattgccaa tatggggata agtaagagta acgatttgac gagaatagga
    57721 aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga cttccccaac
    57781 cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa
    57841 tagcgctgac ttaatcaaaa caccctccag agaatactca ctcactttat ttgcatatta
    57901 attcacagtg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg
    57961 cggcattctg gagatatggg cgctaaaagt caacttaatg gcaggatttt ttatattatt
    58021 ttgtggctca tgcatgtttt acaggcatta agcgacaaaa acatcatcta acctgttctg
    58081 gaaaacgctt gcgcagcaag aagattgcat gaaataaaaa cgagcaggaa aaagtggacc
    58141 aaatggtcaa aatttacatt aaaacaacaa aaatagaaga cgaaacatgt aatttttggc
    58201 agttatagaa aatttaaaag cataacacca aaaataaaag ggctaaaaat taaagccctt
    58261 tataattaat aaattaaatg tgttactttt gcatcataat aaacattttg tggttacaaa
    58321 ttattgagat caagcacatc tcgcatatca aaaagaccac tttccttacc actcaaccac
    58381 aaagccgatc ttaccgcacc gttagcaaat gtcatgcggc tggacgcctt atgggtgatc
    58441 tccagacgct cgccaatatc ggcaaacatc gcggtatgtt cgccaacgat gtcacctgca
    58501 cgcacggtag caaaaccaat ggtacctggc acacgttcac cggtatggcc ttcacgactg
    58561 tagaccgcgc aatctttcag atctttatca agggcgtggg cgatcgcctc tcccattgcc
    58621 agtgcggtgc ctgacggcgc atcaacttta tgtctatgat gtgcttcaat aatttcgata
    58681 tcggtgtagt cacccatcac tttggctgct ttctccagca gcttaagcat gacgttaacg
    58741 ccaacgctaa aattggcggc aaagacaatc gcaatatcgg cggcggcgtc acgaattgcc
    58801 tgtttaccgg cttcgtcaaa ccccgtagtg ccgatcacca tccctttgcc atgctgccga
    58861 caaaaagcga gatggttcag cgtaccttcc ggacgggtaa aatcgataaa cacatcaaaa
    58921 tcatctttta tcgcatcaag actgctttgc acggtaacgc ctgttttccc tgcaccagcc
    58981 agctcaccgg cgtcgctgcc cagtaaagaa gatccttcgc gctccagcgc cgcgcccagc
    59041 tgcacgcctt ctaatgccag tgccgcctga atcaactggc ggcccatacg ccccccggct
    59101 cccgcgatgg caacgcggat gtttgcatca tgcatagcta ttctcttttg ttaatttgca
    59161 tagaccgttt tcagagtaac cagcgcatcg ctacgccgcc agccaaaaac gctgataatt
    59221 agaaattaat gataaacagg gagggatatc agtgaaaggc atgagtcgat gaatgactgc
    59281 atgccgataa catgtgacag gttacggcac cagagccagc acctcagcca cccactgttg
    59341 gaagcctttt acattcagat ccaatgccac ctgtacattg gctggtttgc ccaggcaacc
    59401 gtcgatatca accaccgtcg tgcctgaggt aaattcgcct tgagtttcta ccgcgacaaa
    59461 acacggcttg agggtgaata agtccgggcg caccagccag gcgatggcgc agagatcgtg
    59521 cattcgcaga ccgctttgca tactgccgct acggtagtgg ctaaacaggg cgtggagcat
    59581 ttttccggtg cgattcaact ccggtaaagt agcgagatag tcaggggtta atattgcctg
    59641 attggtgaca tccagaccgc acatgacaat ttcgatgccg ctacggaaga cacaggcagc
    59701 cgcttctgga tcggcagcga tattaaactc ggcatttggt gtacagttgc cgcgaccagc
    59761 agaaccgccc atgatcacca gacgacgaat atacggttta cattccgggc attgtaaaag
    59821 taacagcgca atattggtta acgggccgat ggccaccagg gtaacgggct ctggtgcacg
    59881 catcagggca tcccgaatcg ccagaaacgc cggtatcccg agtggctttc ggttgtgctc
    59941 aacaaagtcg tagccagcca ttcccgattc gccgtgcaca gatgctgcat cacgcggtgc
    60001 gcgtaccagc ggcacagcgg ctccctgggc gagcggaatc tccgcattcc agaaatgcag
    60061 cagttgcagg gcattgcggg tagttttctc aaccgagaca ttacccgcga cggtggtcat
    60121 cagttgcagg tcgagttcgg gggcaaaaat cgcggcggca atggcgacgg catcgtcaat
    60181 gccggggtca gtatcgagga agataggtaa acgcatgttt tcttcataaa aaatgccggt
    60241 aacaagaccg gcattttcgc ataacttagg ctgctaatga cttaatcgac ttcacgaata
    60301 tcgacacgca gctctttcgg cacttcgaaa acaatatttt cttcacggcc ttccagcgga
    60361 atggcttcac caccacccaa ctgctgcaaa cgtgccacca cattctgcac cagaatatcc
    60421 ggagccgatg cgcccgcagt cacgccgacg catttaacct ctttcaccca ctcttcctgg
    60481 atatctttcg catcgtcaat caaaaacgcg tgtttgccca tacgctgggc cagctccgcc
    60541 agacggttgg agttggagga gtttttcgaa ccgaccacca acacaacttc cgcctgttct
    60601 gccagggcgc gtaccgcttc ctgacggtta gtcgtggcgt agcagatgtc atctttgcgc
    60661 ggaccgacaa ttttcgggaa gcgtttacgc agcgcgtcga tcacatcaga cgtgtcatcc
    60721 accgacagcg tggtttgggt cataaaggag agcttctctt cgtttttgac cgtcagtttc
    60781 cacacatcgt ctggcgattc gactagatac attccccctt cggggttgct gtactgcccc
    60841 atcgtccctt ccacttccgg gtgcccggcg tggccgatga gaatagattc ttcgccacgg
    60901 cgactggcgc gggcaacttc catatgcact ttggtcacca gcggacaggt ggcatcaaac
    60961 accgtcaaat cgcggctttt cgcttcgtta cgaaccgcct gagaaacacc gtgtgcggag
    61021 aaaatcagga tcgcgccgtc cggcacttcg ctaatctgct caataaagat agccccacgc
    61081 tcgcgcaagc tatcgaccac gtagcggtta tgcaccactt cgtgacggac atatatcggt
    61141 gcgccgtaaa tggccagcgc gttttcaaca atgctgatag cgcggtctac cccggcacaa
    61201 aaaccacgcg ggttggccaa caggatctgc atgttacgcc tccagtgccg gatcgatttc
    61261 cagcacttca atatcaaaat gaacggtctg cccggccagc ggatggttga aatcaacggt
    61321 aatggagtcg ccgttaattt cgcggatcac accaggcatc tcactgccat ccattgcggt
    61381 aaaaagcatg attgcgccaa tttctggctc gcctgcatcc ataaattcac ggcgggagaa
    61441 gtactgaatc aggtccggtg acggcacgcc aaacgccgca tctggctcca gcgagaaggt
    61501 ggttttgtcg cccactttca gccccagcag gtgttgctcc agcccttcag aaagagaagc
    61561 atcgcccagg cggaacagcg ccggtttacc gttgttgcga gtagactcgg cggtggtgcc
    61621 atcgtcgagt tttagcgtga agtgcaccag gacggcgcta ttgctctgta cagattcaga
    61681 catgcaggtt tgctcttatt tttgaggatt tttgccggat gcggcctacg atttcgtagc
    61741 aatctgtagg ccgcatccgg caggatttat tattgttttt tcgctttgga aggtaaaaac
    61801 ccttccagca cgatcagtgc cgcaccgaca caaatggcag tatcggcaag gttgaaggtg
    61861 gcgaagtgcc agtcgccgac gtagaagtcg atcatatcga caacgaagcc gtgccacagg
    61921 cggtcgaaca ggttgcccag cgcgccgcca ataatcagcg cgtaagcgat attgtttagc
    61981 ttctgcgtgg ccttcgagcg atacatcatc actgccagga gcacgctaat accaatcgca
    62041 ataccggcaa agaaccaacg ctgccagccg ccgctatcgg caaggaaact aaacgccgcg
    62101 ccatagttac gcgcataatg cagattaagc gacgggaaca gcgggaccgt atcccccaga
    62161 gcaaagttct ggaggatcag gtatttgctg cccagatcga taatcagcac gactaccacc
    62221 agccacagcc agcgtagccc tgttgaacag atcgattgac tcatcaggca aacttacgtt
    62281 tttcaccgtc accggcgacg ttgctgacac agcggccgca gatttctgcg tgttccgcca
    62341 ccttgccgac atcctgggtg tagtgccagc agcgtgggca cttctcacct tcggctttac
    62401 tcaacgcgac tttcagccct ttgagtactt cgctctgctg agcatcagca ggtgcgtcgt
    62461 tatagtctgc aacggtagcg ccggaggtca acaggacaaa tcgtaattca tcgcccagcg
    62521 cggtcagttt cgccgccagt tccggttctg catacaaggt tactgccgct tccagcgagc
    62581 cacccacttt cttgtcggca cgcgcttgct caatgacttt gttcacttcg ccacgcactt
    62641 tcaacagctc gtcccagaac gcatcgttca ttgcttcact gtctgccaga ccaaacaggc
    62701 cttcgtacca ctcaccggtg aagacgtatt tttcacgttc gcccggcaga tagccccaca
    62761 cttcatcagc ggtgaaggag aggattggtg ccatccagcg caccagcgct tcagcgatgt
    62821 gatacagcgc agtctggcag ctacgacgcg ccacgctatc cgctttggcg gtgtactgac
    62881 ggtctttgat gatgtcgagg tagaaggaac ccatctcaac ggagcagaag cgcatcagac
    62941 gctgtaccac ttcgtggaaa tcgtatgctt cgtacgcctt gaggatgtct tcctgtgccg
    63001 ctttcgcaca acctacggcc cagcgatcca gtaccaccat ctcttccggt ttcaccatat
    63061 cttttgctgg atcaaaacca ttcaggtttg ccagcaggaa gcgcgcggtg ttacggatac
    63121 gacgatagct atcggcagca cgtttcagga tctcgtcaga aacggccatt tcaccggtgt
    63181 agtcggttga tgccacccac agacgcagaa tatccgcgcc cagtttgttc atcacatcct
    63241 gcggcgaaac ggtgttgccg atggatttag acatcttgcg accctgacca tccacggtaa
    63301 agccgtgggt cagtacctga cgatacggcg ctttgccttt catcgcggtg gagatcatta
    63361 gggaagacat gaaccaacca cggtgctggt cagaaccttc cagatacatg tccgctgcgt
    63421 gaccggcaaa ttccggacgc acgtcaacaa cagaagagtg ggtagatccg gagtcaaacc
    63481 atacgtcgag agtatccggc actttcacat actggtcagc ttcgtcgccg aggatctctt
    63541 tcgcatcgag atcccaccac gcctggatgc cgtcaacttc aacgcgttta gccacttctt
    63601 ccatcagttc gagggtacgc ggatgcagct cttccgtgtc tttgtgcacg aacagtgaca
    63661 tcggtacgcc ccaggtgcgc tgacgggaga tacaccagtc aggacggtta gcaaccatcg
    63721 actcgatacg cgcctggccc cagtccggga gccactgcac gcctttgatc tctttcagtg
    63781 actgcgcacg cagacctttc tgatccatgc tgacgaacca ctgcggtgtc gcgcggaaga
    63841 tgatcggcgt tttgtgacgc cagcagcacg gatagctgtg ctgcattttc tcaacgtgca
    63901 gcagcgcgcc tttttcctgc agcagcgcaa cgacgatgtc gttcgctttg aagacgttca
    63961 cgccatccag cgtcggataa gtgcccggca gataagtgcc gtccgggcca accgggttag
    64021 cggtttccag gccgtatttc tgaccgatca catagtcgtc cgggccgtgg ccaggcgcgg
    64081 tgtgaacggc accggtaccg gcatccaggg taacgtgatc gccgaggatt gccggaacgt
    64141 cgaagcccat aaacggatgg gtaaagcgca gcagctcaag ctctgcacct tttaccgtgc
    64201 cgagaatggt gtaatcggtc acgccgatac gctgcattac gctttcaacc agatctttcg
    64261 ccagaatcac ggcctgaccg tcgatctgca ccagcgcata gtcgaaatca ggtgcaatag
    64321 agattgcgcg gttcgcaggc agagtccacg gcgtagtggt ccagatcacc agcgagattg
    64381 ggccgttaac gttgcttacg ccaaatttcg ctttcagcgc atcctgatcg accgcctgga
    64441 aagcgacgtc aatggacgga gaagttttgt cgtaatactc aacttccgct tctgccagtg
    64501 cagaacggca gtctacgcac cagtgcaccg gcttcgcgcc tttgtgcagg tgaccattgc
    64561 cgatgatttt gcccagcgcg cggatgatgt tggcttcagt tttgaagtcc atagtcagat
    64621 acgggtgcga ccagtcgccc agcacgccca gacggataaa gtctttgcgt tgaccgtcaa
    64681 cctgggtcgc cgcgtattcg cggcacttgg cgcggaactc ggcggcggtg aatttctcac
    64741 ccggcttacc gtattcttgc tctactttca gctcgatcgg cagaccgtgg cagtcccagc
    64801 caggcacata cggcgagtca taaccggaaa gccctttgga cttcacgata atgtctttca
    64861 gaatcttgtt aaccgagtga ccaatatgaa tgctgccatt cgcataagga gggccatcat
    64921 gcagaatgaa ggtttttttg ccttttttag ccgcacggat gatgccgtac agatcatcat
    64981 cagtccaacg cgccagcatt cccggttcgc gcttggcgag atcgccacgc atcgggaacc
    65041 ctgtttccgg caaattcagg gttgatttat agtcactcat cagattctcg gttccgtgtt
    65101 tcggtttgat tacataacag gcttaagccg gttttgttag cccaaaaaat tcgcgggcgg
    65161 ttaattcatc acgcgcaatc tgcgctttca gttcgtccag cgacgcaaat cgctgctcat
    65221 tgcgtatttt tttacgcagc actacttgta tatggcgacc gtaaaggtcc attgcaacat
    65281 ctaacaaatg cacttccagc tgctggcgaa taccggcaac cgttgggcgt gttccgatgt
    65341 ttgccacgcc gggtaacggc ttttcaccga ggcccagcac ttctaccgca taaacccctt
    65401 tcaccgggga aacctgacgg cgtagcggta cattcgccgt cgggaaacct atagtgcgcc
    65461 ctaattcatc accgtggact acacgcccgg agatggcaaa cgggtgcccc agtaaacttt
    65521 ctgccagagc cagattgtca tcagccagcg cctgacgcac ggcggtgctg ctgatacgca
    65581 caccaccttc gcaaaaagtt tgcgtactgg tgatatcgaa gccgtattcc atgccagctt
    65641 tctgtaataa caagaaatcg ccttcacgac cagcgccaaa gcggaaatca tcacctacgg
    65701 caagaaattt tacgcgcaaa tgcttcacca aaagatcgct gatgaaattt tgcgcggtta
    65761 acgccgcaaa acgcctgtcg aaacgcacgc acagcacgta atcaacgcca cactctgcaa
    65821 ggtaacgcag tttttcccgc agccgggtca gtcttgccgg ggctttatcg gtagcaaaca
    65881 gttccagtgg ttgaggttca aaaagcatca ccatcaccgg taagttgcgc ttgcgccctt
    65941 cttcctgcaa gccctgtaac agcgcgcgat gaccgcgatg cacgccgtcg aaattaccaa
    66001 tagtcagcac acacccttct tgcggggcct ggctgagatt atgtatgccg cgtatcagct
    66061 tcatgtctgg ctcaaaacag tgaaaatcgt ccgagtatac cttgtacagc ggtcaaggtt
    66121 aaccggcgat tgagtaccga aatcagaaag aagtggcttt tcattgccat ggagcaaatc
    66181 acgggaagaa actgaccgcc tgctgcaatt tttatcgcgg aaaagctgta ttcacacccc
    66241 gcaagctggt agaatcctgc gccatcacta cgtaacgagt gccggcacat taacggcgct
    66301 tatttgcaca aatccattga caaaagaagg ctaaaagggc atattcctcg gcctttgaat
    66361 tgtccatata gaacacattt gggagttgga ccttggctaa tatcaaatca gctaagaagc
    66421 gcgccattca gtctgaaaag gctcgtaagc acaacgcaag ccgtcgctct atgatgcgta
    66481 ctttcatcaa gaaagtatac gcagctatcg aagctggcga caaagctgct gcacagaaag
    66541 catttaacga aatgcaaccg atcgtggacc gtcaggctgc taaaggtctg atccacaaaa
    66601 acaaagctgc acgtcataag gctaacctga ctgcacagat caacaaactg gcttaatcgc
    66661 caatttgctg aagctttgtg aaaaagcccg cgcaagcggg tttttttatg cctgctgctt
    66721 ttgtgatgcg tgagagaaca cactgccggt tgccagatag tcaaaagaga catgccgttg
    66781 tactatctga actcttcgtg gcttaagaat cgtagaacca tcggctgata cattctgaac
    66841 aagaatatct actcaatctc cagactcccc cacaaagaat atggatattg tgatacacat
    66901 tgaggtatta aacttaagac cactctattt ttgaattaaa ttcctatata ctttaaccat
    66961 t
//
LOCUS       DAJTUP010000006        66599 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTUP010000006 DAJTUP010000000
VERSION     DAJTUP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.006"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..547
                     /locus_tag="OO468_000398"
                     /pseudo
     CDS             1..547
                     /locus_tag="OO468_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            650..973
                     /locus_tag="OO468_000399"
     CDS             650..973
                     /locus_tag="OO468_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR6257645.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1040..2497)
                     /gene="nuoN"
                     /locus_tag="OO468_000400"
     CDS             complement(1040..2497)
                     /gene="nuoN"
                     /locus_tag="OO468_000400"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR6257646.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2504..4033)
                     /gene="nuoM"
                     /locus_tag="OO468_000401"
     CDS             complement(2504..4033)
                     /gene="nuoM"
                     /locus_tag="OO468_000401"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR6257647.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4197..6038)
                     /gene="nuoL"
                     /locus_tag="OO468_000402"
     CDS             complement(4197..6038)
                     /gene="nuoL"
                     /locus_tag="OO468_000402"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR6257648.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6035..6337)
                     /gene="nuoK"
                     /locus_tag="OO468_000403"
     CDS             complement(6035..6337)
                     /gene="nuoK"
                     /locus_tag="OO468_000403"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR6257649.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6334..6888)
                     /gene="nuoJ"
                     /locus_tag="OO468_000404"
     CDS             complement(6334..6888)
                     /gene="nuoJ"
                     /locus_tag="OO468_000404"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR6257650.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6900..7442)
                     /gene="nuoI"
                     /locus_tag="OO468_000405"
     CDS             complement(6900..7442)
                     /gene="nuoI"
                     /locus_tag="OO468_000405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR6257651.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7457..8434)
                     /gene="nuoH"
                     /locus_tag="OO468_000406"
     CDS             complement(7457..8434)
                     /gene="nuoH"
                     /locus_tag="OO468_000406"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR6257652.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8431..11157)
                     /gene="nuoG"
                     /locus_tag="OO468_000407"
     CDS             complement(8431..11157)
                     /gene="nuoG"
                     /locus_tag="OO468_000407"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR6257653.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11210..12547)
                     /gene="nuoF"
                     /locus_tag="OO468_000408"
     CDS             complement(11210..12547)
                     /gene="nuoF"
                     /locus_tag="OO468_000408"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR6257654.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12544..13044)
                     /gene="nuoE"
                     /locus_tag="OO468_000409"
     CDS             complement(12544..13044)
                     /gene="nuoE"
                     /locus_tag="OO468_000409"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR6257655.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13047..14849)
                     /gene="nuoC"
                     /locus_tag="OO468_000410"
     CDS             complement(13047..14849)
                     /gene="nuoC"
                     /locus_tag="OO468_000410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR6257656.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14943..15605)
                     /gene="nuoB"
                     /locus_tag="OO468_000411"
     CDS             complement(14943..15605)
                     /gene="nuoB"
                     /locus_tag="OO468_000411"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR6257657.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15621..16064)
                     /gene="nuoA"
                     /locus_tag="OO468_000412"
     CDS             complement(15621..16064)
                     /gene="nuoA"
                     /locus_tag="OO468_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR6257658.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16695..17633)
                     /gene="lrhA"
                     /locus_tag="OO468_000413"
     CDS             complement(16695..17633)
                     /gene="lrhA"
                     /locus_tag="OO468_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR6257659.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18552..19769
                     /gene="alaA"
                     /locus_tag="OO468_000414"
     CDS             18552..19769
                     /gene="alaA"
                     /locus_tag="OO468_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR6257660.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            19853..20452
                     /gene="yfbR"
                     /locus_tag="OO468_000415"
     CDS             19853..20452
                     /gene="yfbR"
                     /locus_tag="OO468_000415"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR6257661.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20511..22343)
                     /locus_tag="OO468_000416"
     CDS             complement(20511..22343)
                     /locus_tag="OO468_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR6257662.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22430..23080)
                     /gene="hxpA"
                     /locus_tag="OO468_000417"
     CDS             complement(22430..23080)
                     /gene="hxpA"
                     /locus_tag="OO468_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR6257663.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23091..23585)
                     /locus_tag="OO468_000418"
     CDS             complement(23091..23585)
                     /locus_tag="OO468_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR6257664.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23668..24123)
                     /locus_tag="OO468_000419"
     CDS             complement(23668..24123)
                     /locus_tag="OO468_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR6257665.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24461..25663
                     /gene="ackA"
                     /locus_tag="OO468_000420"
     CDS             24461..25663
                     /gene="ackA"
                     /locus_tag="OO468_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR6257666.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            25738..27882
                     /gene="pta"
                     /locus_tag="OO468_000421"
     CDS             25738..27882
                     /gene="pta"
                     /locus_tag="OO468_000421"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR6257667.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28072..29592
                     /gene="yfcC"
                     /locus_tag="OO468_000422"
     CDS             28072..29592
                     /gene="yfcC"
                     /locus_tag="OO468_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR6257668.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29625..30167)
                     /gene="yfcD"
                     /locus_tag="OO468_000423"
     CDS             complement(29625..30167)
                     /gene="yfcD"
                     /locus_tag="OO468_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR6257669.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30225..30776)
                     /gene="yfcE"
                     /locus_tag="OO468_000424"
     CDS             complement(30225..30776)
                     /gene="yfcE"
                     /locus_tag="OO468_000424"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR6257670.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(30832..31476)
                     /gene="yfcF"
                     /locus_tag="OO468_000425"
     CDS             complement(30832..31476)
                     /gene="yfcF"
                     /locus_tag="OO468_000425"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR6257671.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31612..32259
                     /gene="yfcG"
                     /locus_tag="OO468_000426"
     CDS             31612..32259
                     /gene="yfcG"
                     /locus_tag="OO468_000426"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR6257672.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32316..32678
                     /gene="folX"
                     /locus_tag="OO468_000427"
     CDS             32316..32678
                     /gene="folX"
                     /locus_tag="OO468_000427"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR6257673.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            32699..33592
                     /locus_tag="OO468_000428"
     CDS             32699..33592
                     /locus_tag="OO468_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR6257674.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33640..34530)
                     /gene="rpnB"
                     /locus_tag="OO468_000429"
     CDS             complement(33640..34530)
                     /gene="rpnB"
                     /locus_tag="OO468_000429"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR6257675.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(34727..35500)
                     /gene="hisP"
                     /locus_tag="OO468_000430"
     CDS             complement(34727..35500)
                     /gene="hisP"
                     /locus_tag="OO468_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR6257676.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35508..36224)
                     /locus_tag="OO468_000431"
     CDS             complement(35508..36224)
                     /locus_tag="OO468_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6257677.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36221..36907)
                     /gene="hisQ"
                     /locus_tag="OO468_000432"
     CDS             complement(36221..36907)
                     /gene="hisQ"
                     /locus_tag="OO468_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR6257678.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(36997..37779)
                     /gene="hisJ"
                     /locus_tag="OO468_000433"
     CDS             complement(36997..37779)
                     /gene="hisJ"
                     /locus_tag="OO468_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR6257679.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38000..38782)
                     /gene="argT"
                     /locus_tag="OO468_000434"
     CDS             complement(38000..38782)
                     /gene="argT"
                     /locus_tag="OO468_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR6257680.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39048..39617)
                     /gene="ubiX"
                     /locus_tag="OO468_000435"
     CDS             complement(39048..39617)
                     /gene="ubiX"
                     /locus_tag="OO468_000435"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR6257681.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(39712..41229)
                     /gene="purF"
                     /locus_tag="OO468_000436"
     CDS             complement(39712..41229)
                     /gene="purF"
                     /locus_tag="OO468_000436"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR6257682.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41266..41754)
                     /gene="cvpA"
                     /locus_tag="OO468_000437"
     CDS             complement(41266..41754)
                     /gene="cvpA"
                     /locus_tag="OO468_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR6257683.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42013..42675)
                     /gene="dedD"
                     /locus_tag="OO468_000438"
     CDS             complement(42013..42675)
                     /gene="dedD"
                     /locus_tag="OO468_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR6257684.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42665..43933)
                     /gene="folC"
                     /locus_tag="OO468_000439"
     CDS             complement(42665..43933)
                     /gene="folC"
                     /locus_tag="OO468_000439"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR6257685.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44003..44917)
                     /gene="accD"
                     /locus_tag="OO468_000440"
     CDS             complement(44003..44917)
                     /gene="accD"
                     /locus_tag="OO468_000440"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR6257686.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45073..45732)
                     /locus_tag="OO468_000441"
     CDS             complement(45073..45732)
                     /locus_tag="OO468_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6257687.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(45815..46627)
                     /gene="truA"
                     /locus_tag="OO468_000442"
     CDS             complement(45815..46627)
                     /gene="truA"
                     /locus_tag="OO468_000442"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR6257688.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46627..47640)
                     /locus_tag="OO468_000443"
     CDS             complement(46627..47640)
                     /locus_tag="OO468_000443"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6257689.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(47706..48842)
                     /gene="pdxB"
                     /locus_tag="OO468_000444"
     CDS             complement(47706..48842)
                     /gene="pdxB"
                     /locus_tag="OO468_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR6257690.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            48941..49936
                     /gene="flk"
                     /locus_tag="OO468_000445"
     CDS             48941..49936
                     /gene="flk"
                     /locus_tag="OO468_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR6257691.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(49933..51111)
                     /locus_tag="OO468_000446"
     CDS             complement(49933..51111)
                     /locus_tag="OO468_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6257692.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51366..52607)
                     /gene="fabB"
                     /locus_tag="OO468_000447"
     CDS             complement(51366..52607)
                     /gene="fabB"
                     /locus_tag="OO468_000447"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR6257693.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            52766..54772
                     /gene="mnmC"
                     /locus_tag="OO468_000448"
     CDS             52766..54772
                     /gene="mnmC"
                     /locus_tag="OO468_000448"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR6257694.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(54893..55171)
                     /locus_tag="OO468_000449"
     CDS             complement(54893..55171)
                     /locus_tag="OO468_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR6257695.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55205..55753)
                     /gene="epmC"
                     /locus_tag="OO468_000450"
     CDS             complement(55205..55753)
                     /gene="epmC"
                     /locus_tag="OO468_000450"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR6257696.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(55753..56562)
                     /locus_tag="OO468_000451"
     CDS             complement(55753..56562)
                     /locus_tag="OO468_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR6257697.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56562..57386)
                     /gene="mepA"
                     /locus_tag="OO468_000452"
     CDS             complement(56562..57386)
                     /gene="mepA"
                     /locus_tag="OO468_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR6257698.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57390..58475)
                     /gene="aroC"
                     /locus_tag="OO468_000453"
     CDS             complement(57390..58475)
                     /gene="aroC"
                     /locus_tag="OO468_000453"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR6257699.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58510..59442)
                     /gene="prmB"
                     /locus_tag="OO468_000454"
     CDS             complement(58510..59442)
                     /gene="prmB"
                     /locus_tag="OO468_000454"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6257700.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59608..60159
                     /gene="smrB"
                     /locus_tag="OO468_000455"
     CDS             59608..60159
                     /gene="smrB"
                     /locus_tag="OO468_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR6257701.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60263..61153)
                     /locus_tag="OO468_000456"
     CDS             complement(60263..61153)
                     /locus_tag="OO468_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR6257702.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61140..61664)
                     /locus_tag="OO468_000457"
     CDS             complement(61140..61664)
                     /locus_tag="OO468_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6257703.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61661..62131)
                     /locus_tag="OO468_000458"
     CDS             complement(61661..62131)
                     /locus_tag="OO468_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6257704.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62128..62676)
                     /locus_tag="OO468_000459"
     CDS             complement(62128..62676)
                     /locus_tag="OO468_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6257705.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62681..63403)
                     /locus_tag="OO468_000460"
                     /pseudo
     CDS             complement(62681..63403)
                     /locus_tag="OO468_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63423..64145)
                     /locus_tag="OO468_000461"
     CDS             complement(63423..64145)
                     /locus_tag="OO468_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6257706.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64205..65359)
                     /gene="yfcU"
                     /locus_tag="OO468_000462"
                     /pseudo
     CDS             complement(64205..65359)
                     /gene="yfcU"
                     /locus_tag="OO468_000462"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(65308..66063)
                     /locus_tag="OO468_000463"
     CDS             complement(65308..66063)
                     /locus_tag="OO468_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6257707.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66145..>66599)
                     /locus_tag="OO468_000464"
     CDS             complement(66145..>66599)
                     /locus_tag="OO468_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6257708.1"
                     /translation="IEDSVCSIVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQD
                     CVFDTQETMTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGI
                     DQKIVKDTSTNKGKAKQTLNFNAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16564 a        18062 c        16618 g        15355 t
ORIGIN      
        1 cgacacgtaa gtcctgagca attagaaaaa acagttcgtt atcttacaca aactgtgcat
       61 ccacgtaatg ccggcaatat tgataatctg aataggtccg cagagtatat aaaagagttc
      121 tttgtcagta gcggtgccag agttaccgcg cagggcgtcc ccgattggct attacgactc
      181 tgcgcctgga agccagaatt acccttaccc ggcaatgtcc tggctttatc ccgatcgggg
      241 agactttatt gctgtggtcg gcagaatgca ggatatcaac gccgttcgtc aggtaaaagc
      301 ggcattgttg tcatcccggg atttatctgt ttattctatg aatgccccag ggtttattcc
      361 cggtattgat ttttctgatc acctcaatta ttggcaacac gatattcccg ccgtaatgat
      421 tactgacacc gctttttatc gtaataaaca ataccacttg cccggtgata ccgcagacag
      481 attgaattat cagaaaatgg ctcaggtggt agatggtgtt ataaccttgt tatacaacag
      541 taaataataa aaatttctcg aggcgggtcc agaaacagcg acatgtacat tttattttcc
      601 ctcgtctgtt ttaagatgcg aaacataaaa ttcaatggag gaaaaataaa tgggaatgat
      661 tggctatttt gcagaaattg actcagaaaa gattaaccaa ctgcttgagt ctactgaaaa
      721 acctttgatg gataatatac atgacacgct ttcaggatta cgccgactgg aatcactgga
      781 tcgaagtgag ctgcgtaaac agttttccat taagcgactc aatgaaatgg agatttatcc
      841 gggagtgacc ttttcagaag agttagaggg gcaactcttt gcctcgatca tgcttgatat
      901 ggaaaagctg attagcgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt
      961 tattgttggg tgatggtgtc agagcgccaa aaaaaaccgc cgaacatgtc ggcggctttc
     1021 tgacttacaa actaacagtt tacatcagcg gcattgctaa ccgcacaata ctaatcagcg
     1081 gttgtggcca tacacccagc accagtacca acagtgcaga aatcagcacc acgataccgc
     1141 ccgcgctgta ctgccagttt gacggtgcat cgcgacccgg ttgttccggt gcgtgcaggt
     1201 acaggctcac cgccacgcgc aggtagtagt agaggccgat tgccgaaccg acaaccacgg
     1261 cacccaccag ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccga
     1321 taaagcccag cgtcatcggg ataccggcca gagacagcat catcaccgtc atcactgccg
     1381 cgaggatcgg acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc
     1441 cacgatacgg gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat
     1501 aaccggccag gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca
     1561 gcgccaccag cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatgt
     1621 tggtctggct caacgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca
     1681 gtactacgcg aatcgcttcg ctgtcaccca ccggtgcgta gaggaacaga cgcatcacca
     1741 caccgaagat agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc
     1801 cctggtatac gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc
     1861 caacaatcat catgccgaaa cctgccagca acagcggctc gttgagcata ccgtcgccga
     1921 ggtttttacc cagcgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac
     1981 cgaacagcag gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca
     2041 gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt
     2101 caataccgag gaacagagac gccagatggt ttgcattcgc cagcaggatc ccgcccagcg
     2161 cggcaattaa caccaacagg tagaactcat ccttgttgtc gttatagcct tcaagccacg
     2221 ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg
     2281 cgaaaccatc aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccaacaaacc
     2341 agagcgaaac cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat
     2401 tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt caagccgacg atcagcaacg
     2461 gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag
     2521 taacggaatt aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa
     2581 tcggctgcgg atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca
     2641 gctcacgcag cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga
     2701 agtaagcgcg atgcaacatc gccagcgaat aaacagatgc aaagaccagc ccaaaggtag
     2761 agataacggt aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga
     2821 cgaagttacc ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacagtgctg
     2881 gcagccattt catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac
     2941 gttcataaag ctgaccgcag agaataaaca gacccgccgc cgacaagccg tgcgcaatca
     3001 tctggattac cgcgccctgg taggccaact ggctgccggt gtagattgca atcagcacga
     3061 agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgagcgaagg
     3121 tcatccacgc accgtagaag atgccgataa cacccagcca catagcgatt ggcgcgaact
     3181 ctgccgacgc gttcgggaac agcggcaggg agaaacgcaa caaaccgtaa gcggcggttt
     3241 tcagcaagat ccccgcgagg tccacggaac cggcggtcgg agcctgggag tgcgcatccg
     3301 gcagccagcc atgcagcgga accaccggca ttttgactgc gaaggcgatg aagaagccca
     3361 gcatcaacag atattccaca ccactggaca ttggcgtatt cagcagctct tcatagttga
     3421 aggtccagac gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg gcgatcaaca
     3481 tcaccagacc actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt
     3541 taccgtcaga ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca
     3601 tttcccagaa gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc
     3661 ccaggatcca catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg
     3721 aacatagtac cgccagcaca ccgagcagac cggtcagcac gaccatcagc agcgacagac
     3781 cgtcaatggc gagatgaata gagataccaa aacgcgggat ccacggcatg tcgaattcag
     3841 actgccactg cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt
     3901 gcagcgacag cgccagcgtc aatcccatgg tgatcagcgc gatccagcgt ggcaccttga
     3961 cgccaaagcg ttcggtctgc cagcacagga agccgccaat aaagggaatt aatattagcc
     4021 agggtagtaa catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa
     4081 cgaattctca cgacagaaaa ttctcttcgg actgggggta gtgtcggatg cgccgcgaag
     4141 gcagcttatc cgacctacgt tcttatatgc ccaggggcaa tcccacaatc cttaactcaa
     4201 cgcagtacca tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac
     4261 cagcgcagat aaccgttctc gcttaacagc agacctttac ctgcaaagcg ggaaagaacc
     4321 gccgggatgt tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccagg
     4381 aacggcttga cgaacacttt gtcgtacagc cagtcaaagc cccaagcgtt gtaccaccag
     4441 gtactcagca ggcggcccgg cgcactgttg gcgatggagg tcaccagaga acgtttaccc
     4501 agccacagcc aggctgccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc
     4561 agggtcaaca tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgcc ctgcagcggc
     4621 ggtacaatca gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg
     4681 tgagttaccc ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa
     4741 atcatacgga aggtgtagag cgaggtcata aacgcaccga ccagacctgc caccatcaga
     4801 ttgatatgac cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca
     4861 gtgaccagcg gcagtgccga cagtgctgcg ccgcccacca ggaagcagag ataaaccagc
     4921 ggaatagatt tacgcagacc gcccatcttg aagatgttct gttcgtgatg gcaggccaga
     4981 atgacggaac cggatgccag gaacagcagc gctttaaaga acgcgtgagt catcaagtgg
     5041 aaaatcgccg catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc
     5101 atggtagagt aagcgagaac acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc
     5161 agcagcagcg taaccgcccc gacaataccc accagatgca gaacttccgg cgtcatcagg
     5221 aacaggccgt gggtacgggc gatcaggtag acacccgcgg ttaccatcgt tgcggcgtgg
     5281 atcagcgcgg agacaggcgt cgggcccgcc atcgcgtcag caagccatgt ctgcaacggc
     5341 aactgcgcag atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg
     5401 ttattgccgt cagcaaagtg cgctggtgcc agttccacca tatcgcggaa gttcagggtg
     5461 cccagttcgt tgtaaagaat gaacagtgcg aaagcgagga aaacgtcacc cacacgggtc
     5521 acgacgaacg ctttcattgc tgctgcgcca ttcttcggat cggtgtaata gaaaccgatc
     5581 agcagatagg agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttatcg
     5641 gcgagaacca gaactaccat gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag
     5701 tagccctctt caccgcgcat gtaccaggag gcgtacatgt gaataaggaa acccacacca
     5761 gtgaccaccg agagcatggt cagcgacagg ccgtccagca ccaggttaaa accgatgtta
     5821 aagtcgccta ccgacatcca cgtccacagc ggctggctgt atgcctgctc gccgttagcg
     5881 aagaaatcaa cgccgataaa ggcggttacc agcgccgcca ggcccacaga gccaacgccg
     5941 acgatcgccg aaacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca
     6001 atcaatggca aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat
     6061 cgatgttcag gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg
     6121 cttctgccgc cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc
     6181 agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa
     6241 tcagcataaa cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga
     6301 ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt
     6361 cgcgctgtcg tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccgacgtg
     6421 gaaggccaca accagacccg cgagcagcag catagaagcc agttccaccg ccagtacgta
     6481 aggcccgaac agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg
     6541 atcgttaaca ccgaggatgg cgtaaacaat caccaccagc atgatggccg acaaaattgc
     6601 cgggccaatc cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc
     6661 caggttgagc atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat
     6721 aatttccagc gcaccggcga agtaagcgcc tagtgagaag aacaccccgg aaatcgccag
     6781 cagcgaaata atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa
     6841 ggtcgcaagt atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct
     6901 tacggtaaca ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct
     6961 ttgccgtcga ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga
     7021 ccggagatca gcagatcctc tttctcgtaa accagatcct ggcgcttgta ttcccccatt
     7081 tcgaaatccg gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag
     7141 aaaatgcagc gtgagaagtt aatgcggaaa aattccggat accagcgacc gtctttggtt
     7201 tctgcttttt gcagcgagat acagccgacc gggcaggcta ccgcacagag gttacaggct
     7261 acgcaacgct cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg ataacggggc
     7321 ggcagataga ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg
     7381 ccgatcatcc agatactacg aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc
     7441 atggtcttat tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt
     7501 tgatcagcgt cagcggcagg cagattttcc agccgaagga cattacccgg tcataacgcg
     7561 gacgcggtaa cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcagcg
     7621 cgaaccagat gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca
     7681 tcaatgcaga gatggtcaca atcccgatgt actcacccac gaagaacaga ccgaacttca
     7741 taccggaata ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa
     7801 acgggtgacg gtgacacacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt
     7861 gcgggatgac gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac
     7921 cggcctgcgc caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct
     7981 gcgcagaagc acgcatcgca cccagcagcg agtatttgtt gttacttgac cagcccgcaa
     8041 acagcaccgc gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc ccgatgttca
     8101 ggtcggcaac cacccaaccc ggactgactg gcacaattgc aaaggccagc agcagcgagg
     8161 taaaggcaat catcggtgcc agggtaaaga tgacgcgatc cgagaatttc gggatccagt
     8221 cttctttaaa gaacattttg atcatgtccg caaccagctg gagcgaaccg ccccagccaa
     8281 cacggttagg tccataacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca
     8341 tgaatgcccc acaggtgaca accaccagca ggatcaccac cgctttgagg atggtcagca
     8401 ggatctcaat cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag
     8461 atgcgcgcca gccagcaccg gagcaatgcc ggacataccc atcggcaagc ccacctgccc
     8521 tgccgtcagt ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa
     8581 ggagacgcgt gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat
     8641 gtacggctgc ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc
     8701 aaacaggtgg tagtacggcg cgatacgcca tttcccgtcc tgcggctgga agcgtgccgg
     8761 tacgctggtg aagtaatcca gaccgttttc gctggtttca aacagacgca cgcccggatc
     8821 gccaaagcgc agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt
     8881 ccagcccggc gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc
     8941 ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct
     9001 gatattggcg cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg ccagtttctg
     9061 cccacgaata cggaatgtcg catccggcgc agcatctttg atacccgcca gttctgggat
     9121 tttcgccaca acagcgtcaa ttacatggtc gagctgcgtc cagtccactt cacggctcag
     9181 caaggtgctg tgcagcgagt gcagccagcg ccagctttcc agcatgacag ttttgctgtc
     9241 gtaataagcc ggatcgtaaa cctggaagaa acgttgggcg cgaccttcat tattaattac
     9301 cgtaccgtcg ctttcagcaa agctggcaga ggaaagcacc agatgggcgt tttccatgat
     9361 cgctgtgcgt tgatgatcaa ccaccatcac cagcggcgct ttagccagcg cagcattcac
     9421 gcgggtagca gaagcgtaac gatgcagatc gttttccaac accaccaccg cgtcggcgcg
     9481 tccggtttcc agttcggtta acgcttcttc aagcgaaccg ccacccataa tgcccagccc
     9541 catgctgttg acggaacggg caatcatggt gataccaacg tcagcgccgc gacctttcag
     9601 ggctttcgcg acgttagccg cagcctgaat cacctctatg ctaccggcgt tcgtcccgga
     9661 gataatcaac ggtttcttcg cacctgccag tgcctgcacg atgacgtcga ttttgttttg
     9721 cagctcaggt tcgataccgt caaccgctgg tgcggagtta tccagcgcat gggcgatggc
     9781 aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc
     9841 cagacgagtg tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt
     9901 gaggattgcc gcaatctgcc agtcagccac tttctgtgct gcagccattt cgcgcgcttt
     9961 acctttcacc gcctgacgca ctgccagtgc gacgcgcgcg ccggtctggg taacgtcttc
    10021 gcccagcacc agtaccgcat cgtaagattc gatttcgcgc agagccggag tataaatgcc
    10081 gccttcacgc agcactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc
    10141 ggtgtagaag ttttcttcgc ccaccagttc acgcagcgca aagttgcttt ccacgctggc
    10201 acgcggagaa ccaataccga tcactttctt cgactgacgc agaatatctg ccgcgccctg
    10261 cattgcctgt tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg
    10321 cggacgatcc ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta
    10381 gtggtttacc gtaccgttgt aacggttttc gatacgacgc agttcgccgt aacgttcacc
    10441 ggggctgatg ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc
    10501 ccatttacgg ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat
    10561 ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta
    10621 gacgttgtcg tgcgcgccat aaacgcccag atctgtaccg tcagcgtaat ctttgtagta
    10681 acgcacacag cggtagcagg cgatgcagcg gttcatttcg tgagagatga atggccccaa
    10741 atcctgatta cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat
    10801 cacggtcata tcctgaagat ggcagttacc gccttcttca cataccggac agtcgtgcgg
    10861 gtggttggtc attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc
    10921 aatggaaata aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg
    10981 cgtgtcttcc gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc
    11041 cagcgccgga tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag
    11101 caggttgtcc gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag
    11161 catgcttcca gttttctcgg tcagggaccg agcgttaatc gaaattcggt taccagcgct
    11221 ctttcagcag gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga
    11281 ttcccgcctc aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac
    11341 caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg
    11401 tttcgatatc gcccggctgg ccttcgccac gctccagcgc acgcagaatt ttcacgctcc
    11461 acggcagacc gtcgcggcac ggcgtacacc agccgcagga ctcacgggcg aaaaactctt
    11521 ccaggttacg caccagcgac accatgttga tctcatggtc aaccgccatc gccagcgccg
    11581 tacccagacg gctgcccgct ttaccgatac tttcgaattc catcggcaga tcaaggtgcg
    11641 cttcggtcag gaagtcagtc cccgcgccgc ctggctgcca ggctttaaat ttcagaccat
    11701 cacgcatacc accggcgtaa tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt
    11761 cccacagtcc cggatttttc acccgaccgg agaagcccat cagcttggtg ccagcatctt
    11821 tacttttcga gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac
    11881 acagggtttc gacgttgttg acacaggtcg gtttgcccca tacgccggag gttgccggga
    11941 aggggggttt cgagcgtggg ttagcacgac gcccttccag ggagttgatt aacgctgttt
    12001 cttccccgca gatgtagcgc cctgccccgg tatggacgaa cagttcgaag tcgaaacctg
    12061 ttcccataat gtttttgcca agcaagcccg cttcagtggc ttcggcaatg gcgcggcgca
    12121 gatttactgc cgcttcgata tattcgccac gcaggaagat gtagccacgg taagctttca
    12181 gcgcaaacgc ggagatgagc ataccttcca ccagcaggtg cggcagttgc tccatcaaca
    12241 ggcggtcttt ataggtgccc ggctccattt catcggcgtt acacagcagg taacggatgt
    12301 tcatggattc gtctttcggc atcaggctcc acttcaggcc agtcgaaaag cccgcgccgc
    12361 cgcgcccttt cagaccagcg tcttttacct gattaacgat ttcgtccgga gacagcccgg
    12421 tcagcgcctt acgcgcccct tcgtaaccgt ttttgctgcg gtattcgtcc agccacactg
    12481 gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt
    12541 ttttcattta taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt
    12601 gtcctcatcg atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag
    12661 cagcgtaaag cggccatcaa atgtcgtttg ccctggtttg atgttcagct ttttctcgag
    12721 cgccgcctga ataccctgat acccgttgat atgacagacc acgctgtcgc aataacggat
    12781 cacatggcga ccaaccggct ggcggaagat ctgactgtag aacgttgcca caccttcgac
    12841 gtcgcttgcc ggaataccca gcacatcggc gatcgcgtgg atcgcaccat ccggcaccca
    12901 gccacgctgc ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta
    12961 gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt
    13021 ttgtggttgt tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata
    13081 ctgcccagat aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc
    13141 tgcaaatgcg cataactcgg cgtgcggata cgagtgcgat aactcatggt gctaccgtcg
    13201 ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg
    13261 gcaggcatca ccggccccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt
    13321 tgcagcgtgc gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct
    13381 tccggcatgt tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact
    13441 ttcagcatta cgcgggtgta gcagtcagaa acaccaccac caaccgggat ttcaaagtcg
    13501 aagttttcat agccagaata aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca
    13561 cgcaggcccg cgccagtggt gccccactcc agcgcctctt tcgcgccata ggcggcaacg
    13621 ccctgggaac gacctttcag aatggtattt tgcagcgccg ctttctcgta agacgccaga
    13681 cgtttcggca tccagtcgag gaactcacgc agcagacgat cccagccgcg cggcaggtcg
    13741 tgcgctacgc cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc
    13801 accagatcgt aaattttctg acgatcggta aaggcgaaga acacgggcgt cattgcgccg
    13861 acgtcctgaa taaaggtcga gatgtacagc aggtggctgt tgatacggaa cagttcggag
    13921 agcataacgc gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc
    13981 gccagcacgt aaggcatttc gttaacgcag ccgccgaggt attcaatacg gtcggtatac
    14041 ggaatgtagc tgtgccagga ctggcgctcg cccattttct ccgcaccacg gtggtggtag
    14101 ccgatgtctg gtacgcagtc gacaatctct tcgccgtcga gttgtaaaac gatacggaaa
    14161 gccccgtgcg ccgacgggtg gttcggaccg aggttgagga acatgaagtc ctcattttcg
    14221 gtgccgcgtt tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc
    14281 tgtttggctt tggtcagctc aaacggcgag aattcggtag cgcgtgccgg gtaatcttta
    14341 cgcagcgggt gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg
    14401 tcgaaagtaa tgccaaacag atcccaggtt tcacgctcat accagttagc gttcgggaac
    14461 agtttggtga aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg
    14521 tcgcggttac gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac
    14581 ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa
    14641 ggtttcggca gtttctttaa gaaatcgcca acttccagta attgttcacg cttgatccac
    14701 acaacgggaa ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg
    14761 cgcagttcgc caattaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc
    14821 gcggttaagt cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag
    14881 taagcttcgc gaaatagatt taggaataag cgaagaaaaa tccctttgcc gacaggcgca
    14941 aattaaatct cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt
    15001 tcgcgctctg attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc
    15061 ggacgacgtt ctttgccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca
    15121 ggacgcggcg ggcagcccgg gatatacaca tcaaccggga tgaatttatc gacgccctgc
    15181 acaacggaat aaatatcgta cataccacca gagttggcac aggcacccat tgagataacc
    15241 cattttggtt ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag
    15301 caggttcctg caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg
    15361 ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag
    15421 gaaagaccga agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg
    15481 agcttgccca taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc
    15541 tgcttttgca gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa
    15601 tccatcttaa tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc
    15661 atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa
    15721 accagacctg ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg
    15781 ctttcgcgga tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg
    15841 aagaacatgg ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag
    15901 tcgataccgg attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac
    15961 caaccgccta ccagcatcag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat
    16021 gcccagtgat gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa
    16081 gtagcgccag attcactgct cttcacggca gatggacgcc acatcgattc atggggagga
    16141 ataaaaaaaa ccttacaata actgtagaaa ttcttttata cagctaattg atgtggtctt
    16201 ttactccttt ctataacctt ttgtcaactt taacaaaagc ttcttcacat tagtttacat
    16261 aatatcaaca ccattagcat ttaatgccct ttcaccccag atccttgacg actccaggat
    16321 aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt
    16381 gacaggaatt tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg
    16441 cttttgttat tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt
    16501 agcaggtgat ttttttcagg cgattatttg tgcattcggg acgtgaatct ctggtgattg
    16561 aaaaataaac agttttgtac gttctgcact atgcgaggaa ggatttttac taaaaaaaag
    16621 ccgctggggt ttaaaacacc cccagcggct cgttttttac actattgtct caggaattat
    16681 ctatcgtccg tcgattactc gatatccctt tcaatcaaca atgaatcatc cccttccgga
    16741 gcagacattg ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc
    16801 tgggccagct cattattact ggacgggtca tagcacagca aatattcggt atcaggtagc
    16861 ggcggtaagc catctactcc actcagcaca cgcaggtccg ggctcatcat ctcaaccggc
    16921 cttgccgtca cgccaagacc ggctttcact gccgcacgaa cggccggaag cgtcgaggcg
    16981 acataagcca gtcgccatgg aatatctact ttattaagcg tcgccagcac catatcgcga
    17041 aacggacttg gatcatccag caatacaaga gggatcggct cgcctttttg caatacgtat
    17101 tccgctgcgc agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa
    17161 ggacgatggg tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag
    17221 gcattacgtt taacgcggac atccagcgct aatttcggat aaaccgaact cacgcgattt
    17281 aacaggaaag gtaagatcgt atcggcagat tcatctgaag caccgatagt taacacgccc
    17341 tgaagattac tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg
    17401 gcgtaaccaa gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg
    17461 aacagttctt tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga
    17521 gtacgacaca cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat
    17581 gttctcagca gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat
    17641 cacttactgg cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc
    17701 atttgtaatg tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata
    17761 tgagttgcac taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag
    17821 aatatatctt cagggatcgc atatctatta agttactcac tcttttctat ttatgacatg
    17881 cgcgtgtttg tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt
    17941 catacgcgaa cacgtacaaa cagcaatggt gaagcggcat caagagcagg gatccgtcat
    18001 ttatgcgagc attttacccc aaaaaccttt tatttataag ggtcattgcg aattatctga
    18061 tgcaaagtta tgttatgtta ggcaaagtaa tcttctatta ttaataagca catcaaaacc
    18121 tttttgaata ttaaataata attaattagc atcatcctca ttcattaatt ccgcttaaca
    18181 atagtttcac accttacccc cattgcgaca cctcggaaat catcacgctg ttaaactctc
    18241 cgtaatgcgg aaatatcatc atcgcgtttc attagtgaat tcttaaatga ggcattttca
    18301 cacaattatc ttacagataa aaaaaccaga cttgcaatta agaattagaa caattcacta
    18361 tataacattg catgtaaagc atataagcct aattatttgt cattattaag ttattaacag
    18421 cacaatcgag ccttcccctc tggcaaaatc ttattctgca gaccttcaaa acaccgtcct
    18481 gggggagtac attgttctaa gctgacttcc acggcaggga gtggcgataa cagcaaaaaa
    18541 ggtcaagatt catgtccccc attgaaaaat ccagcaaatt agagaatgtc tgttatgaca
    18601 tccgtggtcc ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac aaggtactga
    18661 aactgaacat cggcaaccca gccccattcg gttttgacgc gccagatgaa atcctcgttg
    18721 acgtgatacg caacctgcct accgctcaag ggtattgcga ttccaaaggg ctttactccg
    18781 cgcgtaaagc catcatgcag cactaccagg ctcgtggcat gcgtgatgtt accgtggaag
    18841 atatttacat cggcaatggt gtatcggagc ttatcgttca ggcaatgcag gcattgctga
    18901 acagcgggga cgaaatgttg gttcctgcac cagattaccc actctggacc gcggcggttt
    18961 cgctttccag cggtaaagcg gtgcattatc tttgcgatga atcctctgac tggttcccgg
    19021 acctcgatga tattcgcgct aaaattacgc ctcgtacgcg tgggattgtt attatcaacc
    19081 caaataaccc aaccggcgcg gtatattcca aagagctttt aatggagatt gtggagattg
    19141 cacgtcagca taatctcatt atcttcgccg atgaaattta tgacaaaatt ctctacgacg
    19201 atgctgagca tcactcaatt gcgccgctgg cacctgacct gctgaccatt acctttaacg
    19261 gactgtcgaa aacgtaccgc gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc
    19321 cgaaaaaaca cgccaaaggc tacatcgaag gtctggaaat gctggcttca atgcgcctgt
    19381 gtgctaacgt tcctgcgcaa cacgccattc agaccgcgct gggtggttat cagagcatca
    19441 gtgaatttat tacccctggc ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga
    19501 tcaacgatat tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga
    19561 aaatcgacgc caaacgcttt aacattcatg acgatcagaa aatggtgctg gatttcctgt
    19621 tgcaggaaaa agttctgttg gtgcaaggga cggcattcaa ctggccgtgg ccggatcact
    19681 tccgcattgt cacgctaccg cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc
    19741 gtttcctttc tggttatcat cagctgtaat cttaatttca ctgccggaga ttgcatccgg
    19801 cagtgttatc ccgccacaat gacctgatga tgtcatcata cgtaaggtca ctatgaaaca
    19861 gagccatttt tttgcccatc tctcccgcct gaaactcatt aaccgctggc cgctaatgcg
    19921 caacgtgcgg acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc
    19981 gctggcagct atcaaaaatc ggaaatttgg cggtaatgtc aacgccgaac gtatcgcttt
    20041 actggcgatg taccacgatg cctcagaagt gctcaccggc gatctcccta ctccggtgaa
    20101 atacttcaat gcgcaaatcg ctcaggagta caaggctatt gaaaaaatcg ctcagcagaa
    20161 actggtcgat atggttccgg aagaactgcg ggatatcttt gcgccgttaa ttgacgagca
    20221 tgcatatagc gatgaagaaa aatcgctggt gaaacaggca gatgcactgt gtgcatatct
    20281 gaaatgtctg gaagaactcg cggccggaaa taatgaattc ttgctggcaa aaacgcgact
    20341 ggaagcgacg cttgaagcgc gtcgcagcca ggagatggac tacttcatgg aagtatttgt
    20401 tcccagcttc catctttcgc tcgatgagat tagccaggat tcaccgctgt aagcagccgg
    20461 agtctgcgtc gcatcagtca ataagcgccg gatgcgacat caggctcttg tcaaaacgga
    20521 aacagcatcg ggatcatcac cacacaaacc gccatcacga taatggtgaa cggtaccccc
    20581 aacttcacaa agtcactgaa gctgtaattt cccggaccta aaaccagggt gttaacaggt
    20641 gaagacaccg gtgtcataaa ggcggcggat gctgccatcg ccacgaccat cgcaaatgga
    20701 taaggcgaca ctcccatcgt tttggcagca gccagcgcaa tcggagccat caacaccgcc
    20761 gtcgcggtat tagagataaa cagcccaata accgccgaca agacaaacaa acaccccagc
    20821 atcatatgtg gcccataacc gccgccaata tccatcagcc ctttcaccgc cagcgcgaca
    20881 cctcccgttt tctgtaatgc cacagcaaac ggcatcatcc caacgatcaa aataatgctc
    20941 ggccagtgaa tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc
    21001 aggcaggcga tgatagcggc aacaggatta ggaatttcat ttgtcagcat taacgccacc
    21061 attagcacca gacagaaaat ggcatggggt gcctggctgt gcgcgggcga tgcttcactc
    21121 acctcttccg gtaagttcag cgctacgaag tcgcggccct gtttggccag cataccgatc
    21181 agtttccagt tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc
    21241 gaaccttcca gcgccacgcc attgcgcttg agccccacca cattcagtcc gtagcgggta
    21301 cgaaaaccaa tttcgcgcac cgatttacca atcagttctg actcaggaat taatgaaatc
    21361 tctgccatgc ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc
    21421 tccagcaatt gctcactaca aaattgccgg agatcgacat cagccgcaga catatcaata
    21481 agcaaaacgt cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga
    21541 aaacgccgcc agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga
    21601 tcatccagcc gttgacctat cattggcgat ccggggcgaa tagccagacg tcgcgcacgc
    21661 ccggtcagtc gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc
    21721 tgcggggtct gggtatcccc tttcagcatg aaacgcatca ctaacatata caaaataccc
    21781 agcaccagca cgaccaggcc aataggtgtt acgctaaaga aactgaagcc gtgatagcct
    21841 tcacgcagca attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg
    21901 ccgctaatca gcccggcaaa acttaacggc atcatcagac gtgacggcga agtttgcata
    21961 cgcatggcaa cgcttaacac cacgggaata aagatagcga caacgctggt tgaactcata
    22021 aacgcgccaa gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg
    22081 cccgccactt tgaccagcca tgttcccatt acggtggcaa caccggtacg gaccaaacca
    22141 tcgccaataa taaacaaggc ggcaatcagg acaacgttag gatcagaaaa gccggaaaat
    22201 acttcgggga ccgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc
    22261 gcatccatac gcactctgcc cgtcgcaaac aagacgatgg caacggccag taatgaaaga
    22321 acccaaatca attcaccgtt cacaacttat ccttgttaat tgagggggat gacttgattc
    22381 tgccataaaa aagccccgac gagacggggc taaatcatga tcaggtgttt cactgaataa
    22441 taacatcgcc atttggctgt ttggtcacag taatttgctc cagactgtgg aggaccaaat
    22501 cgacctcatt caggcgcggg gtatctgccg gagcgttaac cgcaatgaca tggcaacccg
    22561 ccgccaggcc agaaagcacg ccagcgggag catcttccac caccacacac tcctgcggcg
    22621 caagccccag cagctgcgca cctaacagat acgcatcagg ttctggtttt ccgcgcttca
    22681 ctcgctcagc ggttacaaac acctctggtg cgggaagccc cgctatttta tggcgcgctc
    22741 gcgctaccgg catggagcca gaagtcacaa tggcccacgg aatacctgct ttattcaaat
    22801 gattgagtaa ggcgattgcc ccaggaagcg cggtaatacc ttcggtttcc gtggcctcga
    22861 tttgctccag acgcgtaaac tcggcggcaa tatcagcctc ggatttgccc gccataaaat
    22921 ggcgcaaaga ggtgatcgcc tgtttaccgt gaatgaaagc cagcacctct tccggcgcta
    22981 acccatgacg tctggcccag ttgctccacg cccgttctac cgcaggcagg gaatccacca
    23041 gcgttccatc aagatcaaac agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg
    23101 ataatttgat taatttcgtt ggcgctcaaa tggtactgac gcgggcaggc atgccacaca
    23161 ttaagcatgc gctgatattt ttcccacatt ggcgtctggg cgttaaaacc atgagtccca
    23221 gcgtcaaaat gggtatagcg cccttccaca ttaaccataa agcggacata accgaggtaa
    23281 cgtgcttcag tggcggcgtc aaagccgagg aaggtgacac gacgttcatc gatggattgc
    23341 tggtcctgca aattagacca agaaacatac aacgcatgat acatatccat aatgtcgatg
    23401 atagtgcggc aggtttcttc tttcagctcg ccaaactcgc gatccaattc acgcatctgt
    23461 aatccgtaac cacgctcaat aattgtttgc aggcgacggt aacgttcagc atttgccgga
    23521 tcgagcatag tcatcatctt gtactgatta gacaaaataa gacgttgcgc gttggtcatt
    23581 tccattgttg actcctgtat cactctacta cggtgaaaaa aaagaaggct gagtatgcct
    23641 tcttttatat gcgtaatcag gggtcaatta caaatcatca aggaaagttt tatccagttg
    23701 tttgaaggcg cgcttaagcg tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg
    23761 tgccaacacc tgtccagact cctgcaattt accgcgaact tcataaaacc agttaaggat
    23821 tgcagggggt aatggcgtta cagaacgctt gcccagccac cacaatccct gcatgggtaa
    23881 acttaaggcg aacagtgcag tggcaactgc cggcccaagc agaccgccca gggcaatctg
    23941 ccagcagaga gtaaatacgg cgatcggcgg cataaaacgg atcgcataac gcgtcatctt
    24001 gataacgcga ttttcgacaa agaccggagc aaggcgtttt tccagtggcc acgtctttga
    24061 gtaatgctgt ccgcggcgaa acaagctaaa aaaattaaca gaacgattat ccggcgttga
    24121 catgcttcac ctcaacttca catataaaga ttcaaaaatt tgtgcaaatt cacaactcag
    24181 cgggacaacg ttcaaaacat tttgtcttcc atacccacta tcaggtatcc tttagcagcc
    24241 tgaaggccta agtagtacat attcattgag tcgtcaaatt catatacatt atgccattgg
    24301 ctgaaaatta cgcaaaatgg catagactca agatatttct tccatcatgc aaaaaaaatt
    24361 tgcagtgcat gatgttaatc ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc
    24421 ctgacgtttt tttagccacg tatcaattat aggtacttcc atgtcgagta agttagtact
    24481 ggttctgaac tgcggtagtt cttcactgaa atttgccatc atcgatgcag taaatggtga
    24541 agagtacctt tctggtttag ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa
    24601 aatggacggc aataaacagg aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct
    24661 caactttatc gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc
    24721 tatcggtcac cgtatcgtac acggcggcga aaagtatacc agctccgtag tgatcgatga
    24781 gtctgttatt cagggtatca aagatgcagc ttcttttgca ccgctgcaca acccggctca
    24841 cctgatcggt atcgaagaag ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc
    24901 tgtatttgac accgcgttcc accagactat gccggaagag tcttacctct acgccctgcc
    24961 gtacaacctg tacaaagagc acggcatccg tcgttacggc gcgcacggca ccagccactt
    25021 ctatgtaacc caggaagcgg caaaaatgct gaacaaaccg gtagaagaac tgaacatcat
    25081 cacctgccac ctgggcaacg gtggttccgt ttctgctatc cgcaacggta aatgcgttga
    25141 cacctctatg ggtctgaccc cgctggaagg tctggtcatg ggtacccgtt ctggtgatat
    25201 cgatccggcg atcatcttcc acctgcacga caccctgggc atgagcgttg acgcaatcaa
    25261 caaactgctg accaaagagt ctggcctgct gggtctgacc gaagtgacca gcgactgccg
    25321 ctatgttgaa gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg
    25381 ccaccgcctg gcgaaataca tcggtgccta cactgcgctg atggatggtc gtctggacgc
    25441 tgttgcattc actggtggta tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg
    25501 caaactgggc gtgctgggct ttgaagttga tcatgaacgc aacctggctg cacgtttcgg
    25561 caaatctggt ttcatcaaca aagaaggtac ccgtcctgcg gtggttatcc caaccaacga
    25621 agaactggtt atcgcgcaag acgcgagccg cctgactgcc tgatttcaca ccgccagctc
    25681 agctggcggt gctgttttgt aacccgccaa atcggcggta acgaaagagg ataaaccgtg
    25741 tcccgtatta ttatgctgat ccctaccgga accagcgtcg gtctgaccag cgtcagcctt
    25801 ggcgtgatcc gtgcaatgga acgcaaaggc gttcgtctga gcgttttcaa acctatcgct
    25861 cagccgcgta ccggtggcga tgcgcccgat cagactacga ctatcgtgcg tgcgaactct
    25921 tccaccacga cggccgctga accgctgaaa atgagctacg ttgaaggtct gctttccagc
    25981 aatcagaaag atgtgctgat ggaagagatc gtcgcaaact accacgctaa caccaaagac
    26041 gctgaagtcg ttctggttga aggtctggtc ccgacacgta agcaccagtt tgcccagtct
    26101 ctgaactacg aaatcgctaa aacgctgaat gcggaaatcg tcttcgttat gtctcagggc
    26161 actgacaccc cggaacagct gaaagagcgt atcgaactga cccgcaacag cttcggtggt
    26221 gccaaaaaca ccaacatcac cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa
    26281 cagggtcgta ctcgcccgga tctgtccgag attttcgacg actcttccaa agctaaagta
    26341 aacaatgttg atccggcgaa gctgcaagaa tctagcccgc tgccggttct cggcgctgtg
    26401 ccgtggagct ttgacctgat cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg
    26461 accatcatca acgaaggcga catcaatact cgccgcgtta aatccgtcac tttctgcgca
    26521 cgcagcattc cgcacatgct ggagcacttc cgtgccggtt ctctgctggt gacttccgca
    26581 gaccgtcctg acgtgctggt ggccgcttgc ctggcagcca tgaacggcgt agaaatcggt
    26641 gccctgctgc tgactggcgg ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt
    26701 gctttcgcta ccggcctgcc ggtatttatg gtgaacacca acacctggca gacctctctg
    26761 agcctgcaga gcttcaacct ggaagttccg gttgacgatc acgaacgtat cgagaaagtt
    26821 caggaatacg ttgctaacta catcaacgct gactggatcg aatctctgac tgccacttct
    26881 gagcgcagcc gtcgtctgtc tccgccagcg ttccgttatc agctgactga actggcgcgc
    26941 aaagcgggca aacgtatcgt tctgccggaa ggtgacgaac cgcgtaccgt taaagcagcc
    27001 gctatctgtg ctgaacgtgg tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc
    27061 aaccgtgttg cagcgtctca gggtgtagaa ctaggtgcag gcattgaaat cgttgatcca
    27121 gaagtggttc gcgaaagcta tgttggtcgt ctggtcgagc tgcgtaagaa caaaggcatg
    27181 accgaaaccg ttgcccgcga acagctggaa gacaacgtgg tgctcggtac gctgatgctg
    27241 gaacaggatg aagttgatgg tctggtttcc ggtgctgttc acactaccgc aaacaccatc
    27301 cgtccgccgc tgcagctgat caaaactgca ccgggcagct ccctggtatc ttccgtgttc
    27361 ttcatgctgc tgccggaaca ggtttacgtt tacggtgact gtgcgatcaa cccggatccg
    27421 accgctgaac agctggcaga aatcgcgatt cagtccgctg attccgctgc ggccttcggt
    27481 atcgaaccgc gcgttgctat gctctcctac tccaccggta cttctggtgc aggtagcgac
    27541 gtagaaaaag ttcgcgaagc aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc
    27601 gacggtccgc tgcagtacga cgctgcggta atggctgacg ttgcgaaatc caaagcgccg
    27661 aactctccgg ttgcaggtcg cgctaccgtg ttcatcttcc cggatctgaa caccggtaac
    27721 accacctaca aagcggtaca gcgttctgcc gacctgatct ccatcgggcc gatgctgcag
    27781 ggtatgcgca aaccggttaa cgacctgtcc cgtggcgcac tggttgacga tatcgtctac
    27841 accatcgcgc tgactgcgat tcagtctgca cagcagcagt aatctcgtca tcatccacag
    27901 ctttgcgctg cggatatctg aaccggaaat aatcactatt tccggttttt tattctctta
    27961 atttgcatta atcctttctg attatcttgc ttaactgcgc tgcatcaatg aattgcgcca
    28021 tctcactttg catacttacc actttgtttt gtgcaaggga atatttgcgc tatgtccgca
    28081 atcactgaat ccaaaccaac aagaagatgg gcaatgcccg atacgttggt gattatcttt
    28141 tttgttgcta ttttaaccag tcttgccacc tgggtagttc cggtggggat gtttgacagt
    28201 caggaagtgc agtatcaggt tgatggtcaa acaaaaacac gcaaagtcgt agatccacac
    28261 tcatttcgca ttctgactaa cgaagcaggc gaacctgagt atcaccgcgt acagctgttc
    28321 acgacgggcg atgaacgccc gggcctgatg aacttcccgt ttgaagggtt aacctcagga
    28381 tcgaaatacg ggacagccgt tggcatcatc atgtttatgc tggtgattgg cggcgcgttt
    28441 ggcattgtga tgcgtacagg aaccattgat aacggtatcc tggcgcttat tcgccatacc
    28501 cgcgggaatg aaattctctt tattcctgcg ctgtttattc tgttttcact tggcggcgcg
    28561 gtatttggta tgggagaaga ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc
    28621 cggctgggct atgacagtat taccaccgtc ctggtgactt atattgccac gcaaatcggt
    28681 tttgccagtt cgtggatgaa cccgttttgt gtggttgttg ctcaggggat tgccggcgtt
    28741 ccggtgcttt ctggctccgg gttgcgcatc gtggtgtggg ttatcgccac tctgattggc
    28801 ctgatcttta ccatggtgta cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg
    28861 catgagtccg accgcttctt tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc
    28921 tttggtgact ggctggtatt gattgtcctg acagccgtaa tggtctgggt gatttggggc
    28981 gtgatcgtta atgcctggtt tattccagaa attgccagcc agttcttcac catgggtctg
    29041 gtgattggca tcatcggcgt tgttttccgc cttaacggca tgacggttaa taccatggct
    29101 tcatccttta ccgaaggggc gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc
    29161 aaagggattt tgctgctggt tggtaatggt gaagcaggta atgccagcgt gttaaatact
    29221 atcctcaaca gcattgccaa tgccattagc ggtctggaca acgcggtcgc ggcctggttt
    29281 atgttgctct tccaggctgt atttaatttc ttcgtgacgt ccggttctgg tcaggcggca
    29341 ttaactatgc cgttactggc accgcttggc gatctggtcg gtgttaaccg tcaggttacc
    29401 gtgctggcgt tccagtttgg tgatggcttc agccacatca tttacccaac ctcagcttcg
    29461 ttaatggcaa cgctcggtgt gtgccgggtg gacttccgta actggctgaa ggtgggcgcg
    29521 accctgcttg gactgctgtt tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg
    29581 ggctaccact aaaaatgtta agagccgcaa ttgcggctct tttttcattc tgccgtttca
    29641 gtctctacgg cttcattttt ggcattgcgc ttcatccaca acgccagcgc tttcagcgag
    29701 tctggagtga actcatcgca gcgtgcggtg atttcttccg gcgtcagcca gcaaactcca
    29761 ctgacttcat cttcctgtag ggcgaaggga ccgtgagaga cgcaactgaa caacgcgccc
    29821 cagacacggc aatttttatc ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg
    29881 gcaatgccca actcttcttc cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc
    29941 tgaactacac cgcctgcggt cgcatctaac atgccgggta aaaagtcttt tgtctcggta
    30001 cgacgctgga ccagaatttt gcccatgcca tcatgcacga cgatgtaagt tgcacgatga
    30061 cgcagacact gtgcccgcat ttgttcccgg ctggcttgtg caatgacttc gttctcttca
    30121 ttgacaatat ccacccattc agtacttgcc aaacgacgct gttccattat caggaaacct
    30181 tctttttctg gcgctcttac ggcgcatttt gagttgtggg taaattacgg attaatcgcg
    30241 acctgcgcaa tgatactttg atcattgagt gcgattacac taagtacgtc attatccagc
    30301 atgccataac ccgccggatt accgcctttc ggaatactca ccgagccggg gttgaagtgg
    30361 aaaatctccc cccgctgttc tgccactggc agatgggtat gaccgtacac cagcacatcg
    30421 ttctggttta aagcaggtag attttccggg ccaaaaagat gaccatgcgt caaaaacaga
    30481 cgttgttttt ccagtaatac ctgttgccac ggcgcggtta tcgggaaatg cagcatcatt
    30541 tgatccactt cgctgtcgca gttgccacgc acagcgataa ccttatgtgc cacttcatta
    30601 agccgttcag cgactttggc tggcgcgtaa ccctccggta aagcattacg cgggccgtga
    30661 ttcaacacgt cgccaaggat caccagccac tgggcaccgc tttggacaaa caactccaca
    30721 acacgttccg tcgccggtaa cgacccatga atgtccgatg caaacatcag tttcatcact
    30781 cactcctcgt cgaaaaaacg taagctatga tactggattc tgtcgttgct atcagccaga
    30841 ttgcttcgcc gaaagtgcaa taaaacgctg gacagacgct cgctgccact ggaatgtcgc
    30901 ataatccacc aggcgttctg gcacctcatc gccatgtagt accaggcggt taatcattag
    30961 cgccagatca gtatcagcaa tgcaccattc accaaataaa ttcggctgac ccagtgctaa
    31021 caaatgttct gccatcgcga acagtttctc tgcactggct tttccctcgg ccgttagtgg
    31081 cgcttttttc gcccccgcaa agacaacatc cgtcggacgc tcttcgcgga tgggcatcag
    31141 atcgctgcgc agccaggcct gaatctgtcg tgcacgcgca cgattttcta aatcaagcgg
    31201 ataaatacgt tcccaggtcg gtggcgcaaa tcgatcttcc agatactccg caatggcaga
    31261 agattcactc aactcaaaat catcgatttg taataacggc acacggcgtg tctgaccgta
    31321 accttgccac gtcggctgca aatgttcacc gctgtcgagg tcgatggtct tgatatgaaa
    31381 cgacaggcct ttttcctgca acgccaccca ggcggataac acataagggg agaaaaagtg
    31441 ggcatctgac caaagcgtga tagcgggttt actcattatg tcctcggcaa taaagggggc
    31501 tggctatcaa actatagagc ctttttgtca ctgtcacgtg acgaatactc acgtaagtcg
    31561 ccgcgctttc tatacttgtg gtgttcagat gattaaacaa ccggagctgc aatgatcgat
    31621 ctctatttcg ccccgacacc caatggtcac aaaattacgc tgtttctcga agaagcagag
    31681 cttgattatc gcttgattaa ggtagacttg gggaaaggcg gtcagtttcg tccggaattt
    31741 ttgcgcattt cgcccaacaa caaaattccg gcaattgttg atcattctcc tgccgatggc
    31801 ggcgaaccgt taagcctctt tgaggctgct gccattttgt tgtatctggc tgagaaaaca
    31861 ggactctttt tgagtcatga aacgcgtgag cgcgccgcca cattacagtg gttattctgg
    31921 caggtaggcg gactggggcc gatgcttggg caaaatcatc attttaatca cgcagccccc
    31981 caaaccattc cttacgctat tgaacgttat caggttgaaa ctcagcgtct ttaccatgta
    32041 ctaaacaagc ggctggaaaa ctcgccctgg ctgggaggcg agaactacag cattgcggat
    32101 attgcctgct ggccgtgggt taatgcctgg actcgcctgc gaattgacct cgcaatgtat
    32161 ccggcagtca agaactggca tgagcggatc cgttcgcgcc ctgccaccgg gcaggcactg
    32221 ctaaaagcac aactcggtga tgagcgttcg gatagttaac agaaacaggt tctcgtgtat
    32281 tatttcatcc taagtaaaac aacggagaac ctgcaatggc acaacctgcc gctattattc
    32341 gtataaagaa ccttcgtttg cgtacgttta tcggaattaa ggaagaagaa attaacaacc
    32401 gtcaggatat tgttatcaat gtgacgatcc actaccccgc cgataaagcg cgcactagcg
    32461 aagatatcaa cgatgcgctg aattatcgca ccgtaacgaa aaacattatt cagcatgtag
    32521 agaataaccg tttctctttg ctggaaaaat taactcagga tgtgctcgat atcgcacgtg
    32581 aacatcactg ggtgacgtat gctgaagtgg agatcgataa actgcacgcg ctgcgctacg
    32641 ccgattcggt atccatgacc ttaagctggc agcgttaatc gccatatcgg gaggctgcat
    32701 gaacatagtg atcaccggag ggacgggatt aattggtcgc catttgattc cgcgtttgct
    32761 ggagctgggc catcaaatta cggtagtgac gcgtaacccg cagaaagcca gttccgttct
    32821 cggccctcgg gtgacactat ggcaagggct tgccgatcaa agcaacctca acggcgttga
    32881 tgcggtaatc aacctggccg gagaaccgat tgctgataaa cgctggactc acaagcaaaa
    32941 agagcgtctc tgccaaagcc gctggaatat cacgcaaaaa ctggtcgatt tgattaatgc
    33001 tagcgacacg ccgccgtcgg tactcatttc cggctcggca acgggctatt atggcgactt
    33061 aggtgaagtg gtggttaccg aagaggaacc gccgcataac gaatttactc ataaactctg
    33121 cgcccgctgg gaagagattg cctgccgggc gcaaagtgac aaaacgcgag tgtgcctgct
    33181 gcgaaccggc gtggtactgg caccggacgg cggtattctc ggtaaaatgc tgccaccgtt
    33241 tcgtcttggc ctgggcgggc cgattggttc cggtcggcag tatctggcct ggattcatat
    33301 tgatgatatg gtcaacggca ttctctggct gctggataac gagctgcgcg ggccatttaa
    33361 tatggtttcg ccctacccgg tacgcaatga acaatttgcc catgcgctcg gtcatgcgct
    33421 gcatcgcccg gctattttgc gcgtccccac aaccgccatt cggctgttaa tgggcgaatc
    33481 ttcagtactg gtattaggtg gacaacgcgc gctgcctaaa cggctggaag aagcgggttt
    33541 tgcgtttcgc tggtacgatt tagaagaggc gctggcggat gtcgttcgct gatgtggttt
    33601 acagcaaaca tccgccagtt aactccctgt gttacaggat tagtggcttt gcgcgataag
    33661 atcgtctggt gaaagtcggg tcaccatcat aactaactct ctgtctaaac ctctatccag
    33721 catctcctga gcaatacgca gggcttcttc gtgtttgccc tgcattgcgc cttcttcacg
    33781 taatctgtca gcaatggtca tcagtttctc cttttcttgt ggtgcgcgtt ctgttatctc
    33841 accaataaat gcacggaaac gctgggcatc ccctgtttgt aatacgtaat taaacagggc
    33901 ttttagctgt ctgtcattag tgttccctgt aactaacagc gaaacaattt ggtcgactaa
    33961 tcccaacaga tcgcgctgac gaatatgttt ctgaattaac tccaacagcg ccattttgcg
    34021 gtgttgcata atctcgtcat ccggcaccac ggtaatatcc accaacggaa acgctgatga
    34081 atatattttg cgggctatag caggctcggc aaattcatca agccagcaga gtgaataagg
    34141 ataaggactt ctgcaaccat gataaaacag catcgggatc accaatggaa gctctttata
    34201 gcccgcatca agatggtttt gcattgccgc aatggaataa cgcatcatgc gaaaagccat
    34261 taattcttcc ggcttacttt ggtgctctat cactacataa atataaccca ctccctcctg
    34321 cgttttcaca gaccacaaga ggtcggaata atattgccgc aggtcttcat caataaaact
    34381 gtttggttct agtttaagcg tcgttaaatc acacagtttg cgcagcggcg cgggaagatg
    34441 aatattaata aaattccgcg cggtgtctgg atggcgtaaa aaagatttaa ataccgcatc
    34501 atgcggcgtg gaagttgtcg atattgtcat ggcgttccgt cacccctcaa atttaagatg
    34561 acgcgacaat aaccacgatt aacacccaat gcagcaggca gtttttatct ttggacagcg
    34621 aattcagaag tatttttagc ggactgcaac ttttacattt cagcggaagc tgactcgcaa
    34681 attccccttg aaaatactcc ggcagcgtta gccaccggag aggaaattat ttcagcgatc
    34741 ccttaaggaa ctgttgcaga cgagggcttt gcgggttgcc aaataactgc tccggcgcgc
    34801 cctcttcttc tattttcccc tgatggagga aaatgacgtg agtagaaaca tggcgagcaa
    34861 agcccatttc gtgagtcact accaccatgg ttttcccctc ttctgccagt tgctgcataa
    34921 tacgtaacac ttcgcctacc agttcaggat cgagcgccga ggtaggttca tcaaacagta
    34981 aaacttctgg ttccatcgcc agcgcccgcg cgatagaaac acgctgttgc tgaccgccgg
    35041 aaagatgcac cggatatttc ccctgcgcac gttcgtctat cccgactttc gccagatact
    35101 tcaccgcccg ctcgcgcgct tcctgcttgc tcaggcccaa cacctgaatc ggcgcttcca
    35161 tgacgttttc caacaccgtc atatggctcc agagattgaa gtgctggaat accatcgtca
    35221 ggcgtgtgcg cagtaagcgc agttgatttt tatcggcgac tttgagttga ccgtctttgt
    35281 cgcgcaccag attgatcgtc tggccattga ccacgatcga tccttcactc ggtttttcga
    35341 ggaagttaat gcagcgcaga aaggtacttt tccccgatcc cgacgatccg atgatgctta
    35401 ttacatctcc ggcattcgct tgcagtgaga cccctttcag cacttcgtgt tcgccatagc
    35461 gtttgtgcaa atcgataacg tttaatttat tctcggacat cgtattctca gtgcgttgaa
    35521 gaaggtttca catgctgcaa ccagcgtttt tccgctctgc gaaagaggct gatcaggaca
    35581 taagagatga ttaaatagag caccgcggca atgccgaagg cggtaaaagg ttgatacgtg
    35641 gcggcgttaa tatcgcgggc tattttcagc agatccggca cagtggccgt aaacgccagc
    35701 gcagtagagt gcagcatcag gatcacttcg ttgctgtatg ccggtaacgc aatacgcagc
    35761 gcagaaggca aaataatgca gcgatacatt ttaaaggtcg agaagccata ggctctggcg
    35821 gcttcaattt ccccatgcgg aaccgaacgg attgccccag caaaaatctc ggtagtgtaa
    35881 gcgcaggtgt taagcgtcag cgccagcacg gtacagttca ggccactgcg gaagaaagcg
    35941 ttaaggaatt cggttccctt aacaatctca agcgtgtaca tgccggaata gaacaccagc
    36001 aactgaacat acagcggcgt accgcgaaaa atataggtaa ataaccagat tggaaactgg
    36061 atgtatttat tactggagac acgaccaatc gccagaaaca gcgccaggac tccgcctatc
    36121 actaccgaca aaataagcag ccacagagtg atcgccacac cagtaaagcg ataaccgtcg
    36181 gtccacagca gcggtttcca gtattcatgt aagatttcga tcacaggtca gccctcttca
    36241 cacccacgga gtagcggcgc tcaaggaaca gcagcacacc attggaaacg gtggtgaaaa
    36301 ccaggtaaat cacgccacag acgatggcga aatagaacgg ttcccaggta cttttgcctg
    36361 ccagctgcgt ggctttgacc acatcttcca ggccgagtaa cgaaaccagt gcggtagatt
    36421 tgaggatcac ctgccagttg ttgccaatgc ctggcagggc gtaacgcatc atcgccggaa
    36481 acatgatccg ccgaaacact tgcccgcgag taaaaccgaa cgccgtcgcc gcctctatat
    36541 gccctttcgg cactgccata aacgcgccac ggaacgtttc ggtaaaataa gcaccgtaga
    36601 taaaaccgag agtgataata ccagcgacca tcggatcgat atcaatctgc ccgacgccca
    36661 tcgcctccgt caccatgttt agcgcaatct gcaaaccata gaaaatcagc agcatcaaca
    36721 ccaagtccgg cacgcctcga atcagcgtgg tgtacccttc gaaaatcagc cccgaaagcc
    36781 gattttgcga gagcttaccg ccagcgccaa ttaaaccgat gattacagcg agcactacag
    36841 agctgatagc cagctccagc gtgacgagcg caccctgtaa aataacacct gaaaacccat
    36901 acaacatgct gcctgtcctg tcgtgtgtgg tgaattaccg cctcttgtct ccctccggtt
    36961 acccgaaggg agcgggcttt ctttcaccga tggcgattaa ccaccataaa catcaaaatc
    37021 gaagtacttt ttcgctaatt tctcgtaagt accgtcagcg cgcatttcgg caaaggcttt
    37081 gttcagtgcc tcgcgcagtt cgttatcttc tttacgcagg cccatgccgg tccctacgcc
    37141 aaacagtttt tcatctttaa cagacggacc accgaatttg taatctttac cgacgggttg
    37201 tttgaggaaa ccttcgctgg cagcgacctc atcctggaac gcggcatcaa tacgtccggc
    37261 agtcaggtca gaataaatgt tgtcctgccc ctgatacgag acgatttcaa tgccttttgg
    37321 tgcccagtgt tcattaccga acgtctcctg ggtggtgccc tgcaatacgc ctacccgttt
    37381 gcctttcagc gactcgactg tcggctgaat gtcagaattt ttcgccacca ccagacgaga
    37441 atcggcagcg tacagtttgt cggtgaaggc aatttcttgc tgacgttttt ccgtaatgga
    37501 aagcgatgac atgatggcgt caatcttctt cgcttttaag gacgggatta acgcatccag
    37561 cgggttttcg acaaacgtac attgcgtatt gatgcgcttg cacaactcct tcgccagctc
    37621 gatatcgaaa ccaaccagtt cgccttgtga attttttgat tcaaatggcg cataggtcgg
    37681 gtcggtaccg atgcggatgt tttgcggaat tgcagcaagt gccgcagttg cgctggagaa
    37741 ggccagaacc agagagagcg atagcaccag ttttttcata atgtcctcaa ctgacagtct
    37801 ttttataggg aatatttaca ggtttgttac acttatcgtg ccataaaatt gaccatcaag
    37861 gcaaaatatc ccgccccgtt agacgtatta tttttcatta aaaatgctta agtatgcatt
    37921 tcaatgcacc acgatagtgc aacaaacctg gcgtgcacca tgatgatcat tccatcaggt
    37981 acagcttccc agcgacgtat cagtcaccgt agacattaaa gtcgaaatac tttttcgcca
    38041 tcttgtcgta ggtgccgtcc tgacgcagct cgccaagcgc cttattgaag gcagccgtca
    38101 gttcagcata atctttacgt agccctacac cggtgccatc accgaagtat tttttgtctt
    38161 ttactgatga gccagcaaaa gcgaaatctt taccagcagg ttgcttgagg aatccttcgc
    38221 tggcagcaac ttcatcttgt aacgcagcat ccagacgtcc tgcagccaga tcggaataga
    38281 ccaaatcctg gttggcatag gccaccacat ccacgccttt actacgccag gtctcgttag
    38341 cgtaagcttc ctgggttgat ccctgcagca caccaacatg tttacctttc agtgaatcca
    38401 gcgttggctg aatcggtgaa cctttggcag caatcaaacg agaatctgcg gcgtacagct
    38461 tgtcggagaa ggcaatctcc tgctgacgtt tatcggtaat ggaaagcgac gaaataatag
    38521 cgtcgatttt tttcgctttc agtgagggga tcagcgcgtc aaagtcactg gcaacccagg
    38581 tacatttcac ctgcatccgt ttgcacatct cgttaccgag atcgatatca aagccaacaa
    38641 aatcaccttt agcatctttc gacgagaaca gcgcataggt ggtatcggtt ccgatacgta
    38701 ccgtctccgg tagcgccgca tagctggaag ccgctgcgga gagaccgact aataaagaca
    38761 gagcgagaat cgacttcttc atacataacc ctcaagtgaa aatggctttt ttatgttgtg
    38821 tattgtgttg tgtgtttgca ggctctttca tgcaggtctt atgccatctt gacgacaaca
    38881 gtaacattca acgttaaata tgttaataag acattgcatt attgtcctga agttgaagat
    38941 agcaggtata gcggttgaat cgcagcgttt cgattgaatg gcagaaacaa aatgtcgagg
    39001 atttgatcgc ggttgcaaaa ttgccctgaa acagggcaac agcggagtta tgcgccctgc
    39061 cagcgggcaa agagatcttc aggaagggtt attgcaaact ggtcaagaac acgattaacc
    39121 gtctgattta tcacatcatc aagggattgc gggcgatgat aaaacgccgg aacgggaggc
    39181 ataatcaccg caccgatttc tgccgcctga gtcattaaac gcagatggcc taagtgcaat
    39241 ggtgtttcac gcacgcagag caccaacggg cgacgctctt tcagcaccac atctgccgca
    39301 cgggtcagta agccatcagt atagctatgg acaatgccgg acagggtttt gattgaacag
    39361 ggtaaaatca ccatccccag cgtctggaaa gaaccggaag agatgctggc ggaaatatcg
    39421 cgcgcatcga gcgtgacatc agctaatgcc tgcacttcgc gcagggaaaa atccgtttcg
    39481 agggataagg tctggcgcgc cgcctggctc atcaccagat gcgtttcgat atctgtgaca
    39541 tcgcgcagaa cctgtaataa gcgcacgcca taaatcgcgc cgctggcacc gctgatgcct
    39601 acaatgagtc gtttcataaa tagttgccct gtcagacttg caggcagact ttgcagtatt
    39661 tcggcgggag ttgcaagtca gggtgccaga ccggcaccct cagcgaaggc atcatccttc
    39721 gttatgcatt tcgagatttt ccacttcgtt ctgacgttgc actgctttgg cgtcatcatt
    39781 acgtaacgta tcgaggaaat cgaggtagcc ctgatcaaca tctttggtga cgtagacgcc
    39841 gttgaacacc gagcattcaa actgctggat atccggattt tcagcgcgaa cggcgtcgat
    39901 cagatcgttc agatcctgga aaatcaaccc gtcagcaccg atgatctggc gaatttcatc
    39961 aacttcgcga ccgtgagcga tcagttccgt ggcgctcggc atatcaatac cataaacgtt
    40021 cgggaagcga atttccggtg ccgcagaagc gaggtacact ttcttcgctc cggcttcgcg
    40081 tgccatctcg ataatctgct cagaagtggt gccacggacg atggagtcgt cgaccagcag
    40141 gacgttttta tcgcggaact cggcgcggtt ggcattcagt ttacggcgca cggacttacg
    40201 acgcagctgc tggcccggca tgataaaggt gcggccaaca tagcggtttt taacgaagcc
    40261 ctggcggtac ggtttgccca gaatacgagc aatttccagc gcgatatcac acgaggtttc
    40321 tgggatcggg atcaccacgt cgatatccag atcttcccat tcgcgggcaa ttttctcgcc
    40381 cagtttcgtg cccatattca cacgcgcgct gtaaacggaa attttgtcga taaacgagtc
    40441 cgggcgggca aagtatacat actcaaacag gcacggattg ctgaccggat tgtcagcaca
    40501 ttgacgggta aacaactgcc cttcttcagt gatgtaaatc gcttcgcccg gcgcgacgtc
    40561 acgcaggaaa tcaaagccca gcgtatcgag cgctacgctt tcggaagcga ccatatattc
    40621 tgtacggttc tcgtcaatat cacgttttcc cagtaccagc ggacgaatcc cgtttggatc
    40681 gcggaaagca accataccgt ggccgataat catcgccaca caggcatacg cgccgcggat
    40741 taagcggttt gtggcagcaa tggcagcgaa aatattgtcg gcttccagcg ggtagtggcg
    40801 gaagttgtcc agctcgctgg cgaagatatt aagcagaatt tccgagtcgg aagtggtgtt
    40861 gatgtggcgg cgtttttctt caaacagttt tttacgcaac tcgtgagcgt tggtcagatt
    40921 gccgttgtgg gcaagcgtaa tgccatacgg ggagttaacg taaaacggct gcgcttcaga
    40981 ggcgctggag ctgccagccg tagggtaacg cacatgacca atgcccatat tgccctgcaa
    41041 acgctgcata tggcgagctt caaatacatc gctcaccagc ccgttcgctt tacgcaaacg
    41101 gaagcagtta ttggcatcta tggtgatgat gccggcggca tcctgaccgc gatgctgaag
    41161 caccgttaag gcatcataaa tcgactggtt aaccggcata acaccggcga taccgacaat
    41221 accgcacata cgtctttttc ctcgttaagc cacatctcag agcacttacg ctctgggcaa
    41281 gaaacttgac gagctttgca gataatcaaa aaaccatctg atgataaaac tgaattgcgg
    41341 gatcagctgt gatttgctcc agtcttcact tttcgacacc ccggtaaagg agtcgagaaa
    41401 gaagagaatg gcggcaacaa tcaacacacc gcgcaacgca ccgaaacaga cgcccagcac
    41461 ccgatcggtg cctgacaacc ccgttttctc caccaactgg cctatcacga agttcacgat
    41521 agcaccaacg atcagggtag cgataaacag taccgcgatg gcaatcccat ttcgaaccag
    41581 ttcgtcttca aagcccgtaa accagactga caggtaagtg tagtaatgac tggcaacaaa
    41641 gaaagcacaa ccccatgtca ccagcgataa cgcttcacga acaaagccgt ggatcaggct
    41701 aaccagagag gaaaaagcaa tcaccgcgat tatggcgtaa tcaatccaga ccatatgtgt
    41761 cccacgattt tacgccctgt catcctgttc ggggcgcatt ctaacagaaa aagaaaacgt
    41821 ttgcgtaggg atttccttcc cgcgcatcaa taaaaatggc gctgaaaaaa tattcaacgc
    41881 catcgacttt ttatgccttt gcggcatcgt gcaatgcgtg tcggatgcgg cgtaaacgcc
    41941 ttatccgacc tacggttcta acactgccca agcctgataa gacgcgccag cgtcgcatca
    42001 ggcaagactg tattaattcg gcgtatagcc cattaccacg ccacttaagc cagaaagttg
    42061 cttcaactca cccagcgaac ctttcagctt atctttcgag gcatccggcc caaccagaat
    42121 acgggtaatt ttaccctgca ctggcgtgga tggcgacgta taaacccgat aaccggcacc
    42181 gcgcagctta ccgacaatct cattcacttt atcggcattt ttcagcgcac ccagttgcac
    42241 aacataggct ttacccgttg gcgctgcttt ttcttccacg accggcttcg gttctggctt
    42301 cggcgcaggt ggtacttcaa cctttggttt aggcggctcc accggtttcg gcttcggtgg
    42361 ggcgaccggt gcaggttccg gttcaaactc ggtgttattg gctgcaatag tggccggatc
    42421 gagcgacggt gctgcggcat cacctgcccg cacctcttcc gctgcgcctt ccggcggctg
    42481 cgtcggcaac gcctgggtgg cggctggcat catatcaggc tcatcacgat cgcccgcttt
    42541 cggcaccagc gggatagccg cgaactcatc ctgataatgt tttttctgcc cgtccagcag
    42601 ccctggaagt acaatcaccc ccagcgccac cagcacgatc gtgcccacta accgattctg
    42661 aaacttactt gccaccgctt ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt
    42721 ggaaagaacc acacaccagc acggtgtctt ccgctttagc gtccgccatt gcggcatccc
    42781 atgcctgcgc aacgctatca aatgatttgc cgttacccaa atgctcaagc agttgttctg
    42841 ccgtggcacc gcgcggccct tccagtggcg cacaatacca gtcatcaacc acgcttttca
    42901 accaggccag agttccggca atatctttat catgtagcat accgataacc gccagcacgc
    42961 gcccgttttt cggtagcgct ttcatacgcc cggtgagata ttccgccgca tgtggattat
    43021 gcgcgacatc aaaaataacg cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa
    43081 ttgcgctggc aatcccgtcg cgaatggcat tttcactgac ttccagcccg ctggcacgca
    43141 gtgccgccag cgccgttgcg gcattcggtt gcgggacaag cggcaacggc agattttcca
    43201 gcgtgccgtg agcatcgcta aacgcccagt catgatcggt gacggaatag ttccactcaa
    43261 cgccccgacg ttgtaacagt gcaccttttt cctgcgccac atcagcaatg gtagaaggca
    43321 tttccggctc accgacaatt gccggttttt cgctgcggaa gatgcctgct ttctcgagac
    43381 caatactttc gcgatctgga cccagccagt cggtatgatc cagcgcaata ctggttacta
    43441 ccgcgacatc ggcatcgaca atattggttg cgtccagacg accgcccagc cctacttcca
    43501 gaatcaccac gtcaagttgt gcctgcttga acagccacaa cgccgacagc gtaccgtact
    43561 cgaaataggt cagggaaata tcaccgcgtg ccgattcaat ctccgcaaaa gaggcggtgt
    43621 gggccgattc cggcaattcc tggccctgca cacgtacgcg ctcggtataa cgcaccagat
    43681 gaggcgaact gtagacgccc actttgtacc ctgccgccat cagaatcgac tccagcgtac
    43741 ggcaggtggt gcctttgcca ttcgtacccg caacggtaaa cacaaatggc gctggtttca
    43801 ggacgccaag acgcgccgcg acctggctca cgcgctcaag gccgagatcg atagttttac
    43861 tgtgcaggtt ttccagataa gaaagccacg aagccagagg cgacgcggct tgaggagtgc
    43921 gtttgataat catggtatcc gctgattcgt tacggtgaga atagcaaaag ggcagagcca
    43981 gtggccctgc ccttatcagt tatcaggcct caggttcctg atccggtacc gggggtacca
    44041 ctacgccttc acgcggcgct tcaggattcg gcgctggcag attcatcaac ttcgccagaa
    44101 tgctcgccag tttcaggcgc atttccggac gacggacgat catgtcgatc gcgcctttct
    44161 cgatcaggaa ttcactgcgc tggaatctag gcggcagttt ttcgcgaacg gtctgttcga
    44221 taacacgcgg accggcaaag ccgattaacg ctttaggttc agcgatgttg agatcgccca
    44281 gcatggcgaa acttgcagaa acaccgccca tcgtcgggtc ggtcagcacg gagatgtacg
    44341 gcaagccgcg ctcctgcatt tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg
    44401 acatcagtgc ttcctgcata cgtgcgccac cagaggcgga gaagcagatc agcgggcagt
    44461 tatcttcctg cgcctgctca acggcacgca cgaaacgtgc acccacaaca gaccccattg
    44521 aaccgcccat aaaggcgaac tcgaatgccg cagcgacaac cggcattcca tacagagtgc
    44581 ctttcatcac caccagcgca tctttttcgc cggtttcttt ctgcgcagat gccagacggt
    44641 ctttatactt cttggagtca cgaaacttca gctcatcttt cggctcaagc tcgctaccca
    44701 gctccacaag gcttccttca tctaacaggc tatgcaggcg attacgcgct gtcatacgca
    44761 tgtgatggtc acacttcgga cagacctcaa gattacgttc cagctcagcg cggtataaaa
    44821 cctgaccgca gctatcacac ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg
    44881 gagtaatgtt gcttttaatt cgttcaatcc agctcattag ggacctttct gtctgaacct
    44941 ggttcgatgc cagttttatc tttggggacg cataatgcca tttttgcccc caacagacca
    45001 tgaatgttgc acattaaaac ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg
    45061 cggagttact ttttattttg cggcgcgtgc cgcagcgcgt ttgtgacgga ttatttcgat
    45121 gacgcccggc aaaatagaaa ccacaataat cccgacgatc agcagcttaa ggttatcctg
    45181 aaccatcggg attgtaccga agaaatagcc tgcgtaggta aaaagcagta cccacaacag
    45241 tgcgccgatc acgttataag cagcgaaatg acggtacgac atgtggccca ttcccgcaac
    45301 aaacggggcg aacgttctga cgatcggcac aaaacgggcg agaataatcg ttttgccgcc
    45361 atgtttctca taaaactgat gggttttgtc gagataactg cgacggaaaa ttttcgaatt
    45421 gggattactg aataactttt caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc
    45481 cacaatcgcg gcgatcagca tcagcactac catcatatgg acgttgagat cgttggtttc
    45541 cagtgatgcc aacgctccgg caacaaacag caatgaatca cccggtaaaa acggcattac
    45601 caccagaccg gtttcacaga acaaaattaa gaacaaaatg gcataaaccc agacgccgta
    45661 ctccgcgact agctctgcca gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata
    45721 aatcaggtcc atatttgcct atgccttgta ctcgtcatct ttcaggctgt aactacgttg
    45781 gctacagcct gaaatgttcc gagcattatt ctcgttagtc cgccagaaat agcgggccca
    45841 ttggcggttt tggaagatca taccggtcag ggtaatccac cgcgaccaga tacagccctt
    45901 ccgcttttgc cgttgctgcc gccagcgttc tgtcctttgc cgccagcaac tctgctatcc
    45961 agctctccgg ctggttgtgg gcaccgactt ccatcaggct gccgacaata ttcctgacca
    46021 tatgatgtac aaaggcattc gctttgatat caaccaccac ataaggaccg tgacgcgtga
    46081 cattaatatg cataacgttg cgccacgggg ttcgggactg gcactgcacc gcacggaacg
    46141 aggtgaaatc attctcgccc agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca
    46201 gcggttcgta aaaatgggtt acccctttac tcagtaccgc cgggcgcagc cgatgattgt
    46261 agatgatgta gcgataacgg cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta
    46321 cagctttaac ccaacgcacg gcgatgtcac caggtaaatt cgcatttacg cccaaggtcc
    46381 acgccgcgtc tttgcgctgc gcggttgttt cgaaatgcac cacttgcccg gtaccgtgta
    46441 cccctgcgtc agtacgcccg gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga
    46501 gcgccttttc cagcttctcc tgcacactgc ggacttcatt ctgccgttgc cagccgtaat
    46561 acttactgcc gtcgtactca atgcccagcg caattttata aactggcagt tgttgctggt
    46621 cggacattag tacagatact cctgcaccag tttctcggcg atttttactg ccatcagcgc
    46681 gccgccaaag cgaacgttat cggccaccga ccagaactgg acttgctccg gcataccgta
    46741 gtcattacgc acgcagccaa cagaaagatg cggcgtaccc gaagcatccc ctacctgagt
    46801 cgggaattcg ttctcttcag agagcacaat atcttcgccc tgagcaaacg catcacgcgc
    46861 ttcttctgcc gccagcggac gcagtgcttc aaagttgacc atctgggcat gaccgtagaa
    46921 taccggtgcc tggacgacgc ttgccgaaat catcagccct tcgtcctgca ggattttgcg
    46981 tacttcgtca acgatacgac gttcttcacg cacgctacct tcgctatccg gcaacaacgg
    47041 cagcatattg aacgccagtt gacgaccgaa gaaatcttct tcgtcaatcg gaatgccgtt
    47101 gagcaatttc gcactctgcc ccgctaacgc atcgaccgct tttttgccct gggcggaggc
    47161 tgaaatcagg ctggtaacac tgatacgcga caaaccgccc tgatcgatta acggtttcag
    47221 tgccgccagc agctggctgg tcagactgtc tggtacggcg atgacattcc ggttgcggta
    47281 atcggtcagt acaaacgggt ttacttccgg caccaccagc ggtacgtcgg gttcgagagc
    47341 aaacagtccg ctgctgtcga tcaccaggca acctgagttg gtcgcttctt caacccaggc
    47401 agcggtagct tctttgcctg cgacaaaaaa tgccagctgc gcctgcgtcc agtcgaattc
    47461 agcggcatcc tgcacggtga ttgttttacc accaaagcgc agttgttcgc ctgcgctttc
    47521 gttacgtgcc agtgcataaa tttccccaac cgggaactga cgttcagcca gcgtttcaag
    47581 cagggcttcg cccatagcgc cagttgcgcc caggacggca atgttccagc cttcagacat
    47641 ggtggtttac tccagaaata gcaaagctcc ctgccaatgt tacagcagag agcatgaaga
    47701 agagattaac gtgccggatg atgaacggcg ttaaaaccca gtttacacag caatgatgcc
    47761 gcactggcgt catcacaaat tacatacaga gatgaccatt cacggcgctc aagatagttt
    47821 ttgcgcagtt tatcgaactc acccggtatc ccggcgactt tacgcagcgg tgcgtcatcg
    47881 cggcgcacat catacaccaa atgcaccagc cttttcagcg tcggttgatc gagcggacca
    47941 tgcagcgtaa tgcggccaaa ctcaggcgcg ggcagcaacg tatccagcgc aacgtgttgt
    48001 tcatgcccaa taaacttgct ataggcttca aacacttgcg tcgtgccacg cgctttaccc
    48061 tccagggtat agcctgcgat atgcggcgtg ccgatatcca cttttgtcag caattccaca
    48121 ttaagttccg gctcgccttc ccagacatcc agcaccacgc ttaacttctg gccttcattc
    48181 aggcaggtca gcagcgcggt attatcgacg actgcgccac ggcaggcgtt aatcagaatc
    48241 gctccgggct tcaggctacg gatcagtttt tcatccgcca gatgtagcgt tttgtacgga
    48301 ccatctttaa agagtggcgt atggaaagtc agaatatccg cgcgctggac taactcatcc
    48361 agcgagcgga aatcaccctc atccccacgg tcggcgcgag gcggatcgca aagtaaggtt
    48421 ttaatcccta acgcttccag tcgcgcctgc aatcgacgtc caacgttacc aacgcccacg
    48481 atccccaccg tacggtcgtg cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag
    48541 gagaaaacat attccaccac cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt
    48601 cccgcctgct tcaaccatgc ttcatcgaca tggtcggtcc ccgcagtggc agttccaaca
    48661 aatttaatgg gttttcctgc cagcaaagat tcattcactt tcgtgaccga acgcaccatc
    48721 agcgcatccg cgtctgccag ttgagcgacg gggattggac gcccgggaac cgcgatcacc
    48781 tcacccaaac ggctaaataa gtcgcgggca taaggcatat tttcatcaac aaggattttc
    48841 acgtttgtgt tacctgtatg agacgagagt taaccggaca agtatgccat aatctcgcgg
    48901 ccaggcatac ttgcgaagat ttcaggtata aggatacgta atgatacaac ctatttccgg
    48961 ccctcctcct gggcagccac caggtcaggg agataacctg ccgtctggcg cgggcaatca
    49021 gcctttatcc agtcagcaac gtacttcgct ggaaagctta atgacgaaag tgacctcact
    49081 gacgcaacaa caaagagcag aactgtgggc gggtatcagg cacgatattg gtctgtcggg
    49141 agattcaccg ctgctttcgc gtcacttccc tgccgctgag cataatctgg cgcaacgtct
    49201 gctggccgcg caaaaaagcc attctgcccg ccagctttta gcgcaattag gggagtattt
    49261 acgtctgggg aatcatcgtc aggcggtcac ggattatatc cgtcataact ttggtcagac
    49321 gccgctgaat cagctctcac cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg
    49381 gaagatggtt attccgcaac cacagcagcg cgaggcgacc gaccgtcctt tattaccggc
    49441 ggagcacaat gcgctaaaac agctggtgac caaacttgcg gcggcaacgg gggaacccag
    49501 caaacagatc tggcaatcga tgctggaact ttccggggtg aaagatggcg agttaattcc
    49561 agcgaaactg tttaaccatc tggtgacctg gctacaggcg cgtcagacgc taagccagca
    49621 aaatacgccg acgctggaat cactacagat ggcgctaaaa caacctttag atgccagtga
    49681 actggcggcg ttatcggcat atatccagca aaaatatggc ctttctgcgc aatcatcgct
    49741 ttcttctgcc caggccgagg atattcttaa tcagctttat caacggcggg ttaaagggat
    49801 tgatccgcgt gatatgcaac cgctgcttaa tccttttcca ccgatgatgg acacgttgca
    49861 aaatatggca acgcgtcccg cgctgtggat actgttagtc gcgattatcc tgatgctggt
    49921 ctggctggtt cgttaacccc gacgaaatga cagtatcgtg acaataatac ccagcaccgc
    49981 agagatcgcc ccggcaagaa ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc
    50041 cagcggcccg gagacgccga gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt
    50101 gccgcgaact tgtgagggga cgcgtttaac cacctccacg cccagcgcag gaaagataag
    50161 cgaacatccg gctccggtta acgccgcgcc cgctaatgcg acccatgcac ctggggcttg
    50221 ccagagcagc aacaagccca ccgtttctac aagcagagag acaatcgcca ctttcacgcc
    50281 gccaaaacgg tccggcatcc agccaaacat gacgcgcatc acgacaaatg cgccgccaaa
    50341 cgcggtaaga gtaaagcccg ccatcgccca tcctttgctg gcaaagtaga gcgaaacgaa
    50401 agtcccgata accgcaaaac caacgccttg tagtgccagc cctaaccctg gtttccagat
    50461 aagcccgaca acgctccaca gcgatggacg ttctcccgcc agggccggta ctttgcgcac
    50521 tgtgccgtta caggcccacg ccagtaaggg taataccatt gtggtgatcg ccagtgcggc
    50581 aaaaccgtaa tggctatgaa tcaacaggcc aagcggcgca ccaacagcga gggcaccgta
    50641 aatcgccatt ccgttccatg acatcacttt gccagagtgt tttggcccta cgatgcctaa
    50701 cccccaggtc agagcgcctg tcagtaactg gctttcacca aacccaagaa tcaaacgccc
    50761 gatgaccaac agggcaaatt tgaacggtgc ggagacaggc aaaatcgccg ccagcagcaa
    50821 cgcgccgcca gccagaccac aagctaacat cccctgaagc gccgaacgtt ttgcgccata
    50881 ttgatcggcc agtcgcccgg cgtaaccacg cgtcagcacc gtagccagaa actgaatccc
    50941 gacggcaatg ccgaccatgg tattgccata gcccagatca tgatgaacaa acagcgggat
    51001 aaccggcaac ggcaacccta cggtcatgta ggtgagaaaa accgcaaaag agatgcggaa
    51061 gagcgaaaaa ctggcagaag atcgtgtttc ggtttggctt acagcagtca tgcattactc
    51121 cagaatgcag cgcaaggcga ggagtatccc cgtctcatct ctctggtttc agggttacgg
    51181 tgcgttggca ggatttaacg cgtacgtctt ttcagaagga aatcgacaaa gcgggaagtt
    51241 tgcctagaac tggtggcgat tgtcaatgat gtgaaaaagg gaaccatcag gttccctttt
    51301 gcgttagtgc cggaggccgc attggcgcgt aacgtcggat gcgacgctgg cgcgtcttat
    51361 ccgacctact ccggcctact gcgaattaat cttttcagct tgcgcattac cagcgtggcg
    51421 ttggtgccgc cgaagccgaa gctgttagac ataacagtgg tcagttcgcg atcggtcgtt
    51481 tcggtcacga tgttcagacc cgcagcctgc tcgtccagct cttcaatgtt gatgctcggg
    51541 gcgataaagc cgtgttccag catcagcaga gagtagatag cttcctgcac acccgcagcg
    51601 cccagtgagt gaccggtcat ggctttggtt gcagaaatcg ccgggctctt atcgccgaac
    51661 acttcacgga tagctgccag ctctttcacg tcgccaaccg gagtcgaagt accgtgagag
    51721 ttcaggtaat cgattggggt atcaacgcca tgcatcgcca tctgcataca acgtactgcg
    51781 ccttcgccag acggagcaac catgtctgca ccatcagagg ttgcgccgta gccaacgatt
    51841 tcagcataga tgtaagcacc acgcgccagc gcgtgttcca gctcttcaac cactaccata
    51901 ccgccgccgc cagcgataac gaaaccgtca cggtgagcgt cgtaagtacg ggaggctttt
    51961 tccggggtgt cgttgtattt agtagatagc gcacccatcg cgtcgaattc gcaggccatt
    52021 tcccagcaca gctcttcgcc gccgccagca aacacgatgt cctgtttgcc cagttggatc
    52081 tgctctactg cgttaccgat acagtgtgcg gaagtcgcac acgcggagct gatggagtag
    52141 ttaacgccat gaattttaaa cggcgtggcg agacaggcag aaacgccgga tgccatcgct
    52201 ttagtgacca catacgggcc aaccgctttc aggccgcgcg ggccgcgcat tgcgtcagcg
    52261 ccgaacacct ggaaacgcgg ggaaccgccg ccggaacctg caatcaggcc aacgcgcggg
    52321 ttattctggt aagcttccgg agagaggccc gcatcagcga ttgcctgctc catagaaagg
    52381 aatgcataaa tggatgcgtc gctcataaag cgcacaactt tgcggtcaat gaggccagtg
    52441 gtatccagtt ttatgttgcc ccagacgtgg ctacgcatgc cggaatcctt cagctcctga
    52501 gagaaagtga tccctgaacg tccttcacgc agagatgcca ggacttcctg ctggttatta
    52561 ccgatgctgg aaacaatgcc caggccagta atcactgcac gtttcattca atacctctgt
    52621 aagtcgcaca tagagtaagt ttcgaatgca caatagcgta cacttgtacg ccgaacaagt
    52681 ccgatcagcc atttaataga gatatttgcg cagccttaca cacatcgcta agatcgagcc
    52741 accgcctgta agacgagtaa cttacgtgaa acactactcc atacaacctg ccaacctcga
    52801 atttaatgct gagggtacac ctgtttcccg agattttgac gatgtctatt tttccaacga
    52861 taacgggctg gaagagacgc gttatgtttt tctgggaggt aaccaattag aggtacgctt
    52921 tcctgagcat ccacatcctc tgtttgtgat agcagagagc ggcttcggca ccggattaaa
    52981 cttcctgacg ctatggaagg catttgatca gtttcgcgaa gcgcatccgc aagcgcaatt
    53041 acaacgctta catttcatta gttttgagaa atttcccctc acccgtgcgg atttagcctt
    53101 agcgcatcaa cactggccgg aactggctcc gtgggcagaa caacttcagg cgcagtggcc
    53161 aatgcccttg cccggttgcc atcgtttatt gctcgatgaa agccgcgtga cgctggattt
    53221 atggtttggc gatattaacg aactgaccag ccaactggac gattcgctaa atcaaaaagt
    53281 agatgcctgg tttctggacg gctttgcgcc agctaaaaac ccggatatgt ggacgcaaaa
    53341 tctgtttaac gccatggcaa ggttggcgcg tccaggcggc acgctggcga catttacgtc
    53401 tgccggtttt gtccgccgcg gtttgcagga agccggattc acgatgcaaa aacgtaaggg
    53461 ctttgggcgc aaacgggaaa tgctttgtgg ggtgatggaa cagacattac cactcccctg
    53521 ttccacgccg tggtttaatc gtacgggcag cagcaaacgg gaagtagcga ttatcggcgg
    53581 tggtattgcc agcgcgttgt tgtcgctggc gttattacgg cgcggctggc aggtagcgct
    53641 ttattgcgcg gatgaagccc ccgcgctggg tgcttccggc aatcgccagg gggcgctgta
    53701 tccgttatta agcaaacacg atgaggcgct aaaccgcttt ttctctaatg cgtttacttt
    53761 tgctcgtcgg ttttacgacc aattacccgt taaatttgat catgactggt gcggcgtcac
    53821 gcagttaggc tgggatgaga aaagccagca taaaatcgca cagatgttgt caatggattt
    53881 acccgcagaa ctggctgtag ccgttgaggc aaatgcggtt gaacaaatta cgggcgttgc
    53941 gacaaattgc agcggcatta cttatccgca aggtggttgg ctgtgcccag cagaactgac
    54001 ccgtaatgtg ctggaactgg cgcaacagca gggtttgcag atttattatc aatatcagtt
    54061 acagaattta tcccgtaagg atgactgttg gttgttgaat tttgcaggag atcagcaagc
    54121 aacacacagc gtagtggtac tggcgaacgg gcatcaaatc agccgtttca gccaaacgtc
    54181 gactctcccg gtgtattcgg ttgccgggca ggtcagccat attccgacaa cgccggaact
    54241 ggcagagctg aagcaggtgc tgtgctatga cggttatctc acgccacaaa atccggcgaa
    54301 tcaacatcat tgtattggtg ccagttatca tcgcggcagc gaagatacgg cgtacagtga
    54361 ggacgatcag cagcagaatc gccagcggtt gattgattgt ttcccgcagg cacagtgggc
    54421 aaaagaggtt gatgtcagtg ataaagaggc gcgctgcggt gtgcgttgtg ccacccgcga
    54481 tcatctgcca atggtaggca atgttcccga ttatgaggca acactcgtgg aatatgcgtc
    54541 gttggcggag caaaaagatg aggcggtaag cgcgccggtt tttgacgatc tctttatgtt
    54601 tgcggcttta ggttctcgcg gtttgtgttc tgccccgctg tgtgccgaga ttctggcggc
    54661 gcagatgagc gacgaaccga ttccgatgga tgccagtacg ctggcggcgt taaacccgaa
    54721 tcggttatgg gtgcggaaat tgttgaaggg taaagcggtt aaggcggggt aatctgctct
    54781 cgcagtgttt gccggatgcg gcgtgaacgc cttatccaac ctacgtgtga cgtgtaggcc
    54841 tgataagacg cgataagcgt cgcatcaggc atggtgctcc agacgccgca acttacagct
    54901 gtgacgcctg ctgaaacagg ttttcccaca tatcggtcac cagcgcctgg tcacgcggcg
    54961 acaattcacc ggcaccaatg gctttttcca gactctggct aacggtcgta tgcaccgcct
    55021 gagcggagtg gtcatcacca ctttccagtt ctgcgatggc taatgtcagg tggccacgca
    55081 aatacccact ggcaaacaac tcatcatcac tggcatggtc aaccatgccg tcgattaatg
    55141 ccagaatgcg tgattcaaac tccgcgatca tcttctttcc tcattgtaaa acgtggcgct
    55201 ggcttcagtt gagcgcttcc ggccacggga actgttccgc cgtaagttcc ggcgtgtgat
    55261 aataattttg tagtgcttta atgaagcgtg ccgggcgttc cggaatgcca tttgtcagat
    55321 aatccatcac ctgcgcatgt acccgccgtt gaaacaccac acgatccggt tcgaaatccc
    55381 cttccagatt gtcgcaactg acgttaaacg gatatccagc cgccacgcag aataaccagt
    55441 cgaacgcctg cggcttcact tcaacatctt caaactggct ttgcgtctgg gcatcgcgtc
    55501 cgtccgggca ataccagtag ccgaaatcaa ccagctcacg gcgcgctttt ccggcaatac
    55561 accagtgcga aatctcatga attgcgctgg cataaaagcc atgagcaaag acgattcggt
    55621 tatacggtac ttccgcatca gcaggaagat agatcggttc gtcgtcgcct ttaatcagac
    55681 gggtattaaa ttcatcggca aagcagctat taaaaatttc aatcaactgc tcgtagtggt
    55741 gtgtactgtt cattagttca tccccaacca gtggaggatc tcctgtccgt ggctatcgta
    55801 aagtaatttg gcactcatca ccgccgagac gataacaatc atcgggcgga tcagcttttg
    55861 tcctttgctc aacaccaacc gtgaacccat gcgcgcgccg aggaactgcc ccaccagcat
    55921 gacaaagccc gtcgcccaaa tcactttgcc gccgagaata aacagcagca gaccgccaat
    55981 gtttgacgtt gcgttgagta atttggcgtg agccgtggct ttggcgaggt tgaatccgca
    56041 cagcataacg aaggccagcg cgtaaaacga tccggcagcc gggccaaaga atccgtcata
    56101 aaaaccgacg cagccaccgg caattaacgc gaagggtaaa ccgtacatcc ggcgctggcg
    56161 atcttcttca cccagctttg gcatcagcaa gaaatagagg ccgatacaaa tcaccagaat
    56221 gggcaaaatc tgccgtaaga catcagcctg aacgtactgt accaacagtg cgccgctcat
    56281 tgagccgaca aaggtcatgg caatattgag tttctgatcg cttaaactaa ccactttgcg
    56341 gcgaataaag tagatagtgg cggaaataga gccgccgcag gcttgcagtt tattggttgc
    56401 cagcgcgtta gcgggagaca ttcccgcagc catcaatgcc ggaatggtga gtaacccacc
    56461 gccaccggca atcgagtcga taaatcccgc cagcatggcg acaaaaaaga gaactcccag
    56521 caacagcgga gaaaccataa acaggctatt aaacgtttcc attagatcac gtgctcatcc
    56581 agtagcgcct ggcaggaagg cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt
    56641 cccggttttg gaggttcaaa ccagctttgc agttctgccc cgcaaccatc gcctgatggc
    56701 ggtaaaggtt gatcttcaca ctccagacta tcggcagggc aacgtaatcg tacatgcata
    56761 tgcgcgcgat gctggaacca gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca
    56821 tcaaggcaaa gttgttgttt aatcgccgga ttaacaaaaa tgcgcgtgac gtctttgtcc
    56881 tgggcggcga gtttgatcaa gctgaaaatt tctggcttcc acagcgtgga gacaacgtgt
    56941 ttaccgtcgc gggaaactaa gtccagtgct tgcgggcgca agagctgcgc ggaggtccag
    57001 cgagttttcg gcagttgcag aaagatatcg acatccagtc cggtctggtg gctggcatga
    57061 ccgccgttga aacgcccacc agcgggcatc cccatatcgc caatcagcac cgtacccatg
    57121 cccagattgc tcacctggct actcagacgc tggataaaca tcaccagatc cgggtgaccg
    57181 aaatagcgac gctgatcggt acgcatgacc tgataatgtt cggactgtat cggcaacgtg
    57241 tcagcgccga caatacagcc attagaaaaa ctgcctatcg attgtgcgct acccggcaca
    57301 ggttgggtta ttttttgcca cggcgtcgct gccaggctag cactactggc aagcagagcc
    57361 agcagcgcaa tcgcggtttt gttcattttt taccagcgtg gaatatcagt cttcacatcg
    57421 gcattttgcg cccgctgccg taacaggtga tccattaaaa cgatcgccag catcgcttca
    57481 gcgatcggca ctgcacggat cccgacacag ggatcgtgac ggcctttggt gatcatctca
    57541 acttcttcgc caaagcggtt aatagtgcgc cccggcacgg taatgctgga ggtcggtttc
    57601 agcgccatat gagcaatgat ttgctgcccg ctgctgatac cgccgaaaat gccgcccgca
    57661 tggttgctct ggaaaccgtc tttggtgatt tcgtcgcggt tctggctgcc gcgcagcgcc
    57721 actacgtcaa aaccgtcgcc aatttccacg cctttcaccg cgttgatgct catcagcgca
    57781 tgggcgatgt cggcatccag gcggtcaaag accggctcgc caagtccggc ggggacacca
    57841 ctggcaacaa cggtgacttt cgcgccgatg gagtcgccct cttttttcag cgcgcgcatc
    57901 agttcatcta acgcgtcgat tttgtcgggg tccgggcaga agaacggatt ttgttcaacc
    57961 agcgaccagt ctttgatttc cagcggaatg tcgcccatct gggtcaggca gccgcggatt
    58021 tcaataccaa atttctcggc gagatatttt ttggcaatcg cccctgccgc cacgcgcatg
    58081 gcggtttcgc gggcggaaga acgtccaccg ccgcgataat cgcgcagacc gtatttttgt
    58141 tcgtaggtgt aatcagcatg gcctggacgg aagacgtctt taattgcgct gtaatcctga
    58201 gaacgctggt cggtattttc gatcaacaag ccaatgctgg tgccagtagt aacgccttca
    58261 aaaacaccgg agagtatttt gacctgatcc ggctcgcggc gctgggtggt atagcgcgat
    58321 gtcccagggc gacgacgatc aaggtcatgt tgcaggtccg cttccgtcag cggaatgcct
    58381 ggcggaacac catcgacgat gcagccgagc gccagcccgt gcgattcgcc gaaggtggtt
    58441 acgcgaaaga gttgtccaat tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg
    58501 cgtgtttact taatctttat aaatcgcgaa atgttctcgt gcggcaataa gctgctcttt
    58561 agtgagcata aacacaccat cgccgccgtt atcaaactcc agccaggtga acggaacatc
    58621 gggatattgt tccataagat gtaccatgct gttgcccact tcacaaatca acacgccatc
    58681 atcagcaagg taatctgccg cgttaccgag aatgcgacgc gtcagtttca ggccgtcggt
    58741 gccagatgcc aggcccagtt ccggctcgtg acggtattcg tttggcaggt cggacatatc
    58801 ttccgcgtcg acatacggcg ggttagtgac aatcagatcg tattgcactt tcggcaaatc
    58861 acggaacaga tcggaacgaa tcggaatgac gttgtggatc agaccgtgtt cttcgatgtt
    58921 ctgttcggca accgccagcg cgtcgggaga gatatctacc gcgtcgactt ccgcttccgg
    58981 gaaggcataa gcacaggcaa tggcgatgca gccgctaccg gtacacatat ctaaaatatg
    59041 ctgcggttgc ttgctgatga gtccggcaaa tttattgttg atcagttcac caatcggcga
    59101 gcgcggcacc agcacgcgtt catcgacgta aaattcatga ccgcagaacc aggctttgtt
    59161 ggtcaggtaa gccaccggaa tgcgctcgtt gacgcggcgg atcacgcgtt caacaatacg
    59221 gtgtttttcg ctggaggtca gacgcgcggt gcgcatatct tccgggatat ccagcggcag
    59281 gtagagcgat ggtaacacca gctgtacggc ttcatcccac gggttatcgg tgccgtgacc
    59341 gtaccagata tttgccgcgc tgaagcggct taccgaccag cgcaatatgt cctgaatggt
    59401 ttgcagctca tttactgctt catcaacgaa aattttatcc acgtattcct ccagggcatg
    59461 atcgcaataa tttcggcggc tagtttgcca tgaagatgac gataaatcag cattcacgcg
    59521 cggtgagtga ggaaaaatac gtttaaaacg atcgattgtg ctacgagtcg ggtaaactgt
    59581 aggaaaatta gaaatagaga cagataaatg aaaaagaaaa caacacttag cgaggaggac
    59641 caggctctgt ttcgccagtt gatggcgggg actcgcaaga ttaagcagga cacgattgtc
    59701 caccgaccgc agcgtaaaaa aatcagcgaa gtgccggtga aacgcttgat ccaggagcag
    59761 gctgatgcca gccattattt ctccgatgag tttcagccgt tattaaatac cgaaggtccg
    59821 gtgaaatatg ttcgcccgga tgtcagccat tttgaggcga agaaactgcg ccgcggcgat
    59881 tattcaccgg aattgttttt ggatttacac ggtctgacgc agctgcaggc caagcaggaa
    59941 ctgggcgcgt tgattgccgc ctgtcgccgc gaacatgtgt tttgtgcctg cgtgatgcat
    60001 gggcacggga agcatatttt gaagcaacaa acaccgctgt ggctggcaca acatccgcat
    60061 gtgatggcat ttcaccaggc accgaaagag tacggcggtg atgcggcttt gttggtgttg
    60121 attgaagtgg aagagtggtt gccgccggag ttgccctgaa tgctggcagc aaatgccgga
    60181 gcagcgtaaa cgccttatcc ggcctacaaa tatgtgataa ttcaataaat tgcaattcac
    60241 ttcgtaggcc tgataagacg catcagagca atactacgaa cttcagttca ttcaatacat
    60301 actgaaaacg acacgtcgaa tcaatcggtt gcccactact ggtattcaca ttcaccacaa
    60361 tatcgtgtag tactttatcc gttaccggat gacgagttgg cgtaaccgtt accgttgatt
    60421 tatcacaacc gtaagaggta aaggttaaca attctgatac cgcgcggcag tatgctcctc
    60481 tgccatcatt atcagaaaaa ttactgccat cacagagtct gccgccttcc atcaggtagg
    60541 tcgaaagcgt ggtggtcact gttgaccccg tggactgaat tcgtaacgcc ttattatggc
    60601 tgacataatt ttcactccag gagatatcgt atgaagcgac cggtatcgtt gggcatattg
    60661 tgttatgcac tgtaggggcc gtatagcttc caaatccggg taaatctgta taggaataaa
    60721 aggatgcgat ccaagggcaa ccataatcat cagatgcaac ggtaatttta aacgtcatat
    60781 atgcacaacg aaaaattttc gtatctatgc cagggcaatt tgagttatta attgtatcaa
    60841 agtcagtcag taccaaagtt ggagctgaac ccgacccaga ccaggaaata acccgcaacg
    60901 tatcgccttt atacgcaaaa gaatcatatt tcccatatgc aacctccttt ggcgttatca
    60961 ccgtaacatt gaaaacactg ctcgctacag caccggttac cttgtccatg gcataataaa
    61021 ctctcacact gtcaaaactc gcactcataa gtttatctcc agcataactc ttagatgcgc
    61081 cgacaacact cagaacgagc agcattaggc tcgcgaaaat tatggtccat tttttcattt
    61141 cagaaatact caaaggttgc cgtcactgtg gcactaaagt ctccggacgt tacatctcgc
    61201 ccgctctttg cctgtaacca ggtattaaaa tttaggctgt tatttccctg attcagcggg
    61261 agcgtgacgc ctgttgtatt gttgattggc accgatgtgc ctgccgcagt ttcaatacca
    61321 atcccagcgc cggatgctga tgacgaggag tcaaaggcca gaaagccggg tagagcacta
    61381 tcttctgttc ctgtcaacgt tactttgaca ttaaacaatg tagaactatg acaatctttc
    61441 aattgaaaca ccaatggcac tcgctctgac tgcccaaggt tcattaaatc actggcggca
    61501 atggcaggaa aatgtacttc tgccaactgg ctaccattaa tcactggcgt acacgattta
    61561 ctgattaaat tgccggtaaa gtgcaaatta tctcctgcta ttgtggttcc actgcccagc
    61621 aacatcaggc tactaataaa aaacaacgta cgctttttct tcattggtaa tctacctgta
    61681 acgtggccca ggcctcaaag tccgcctctg gcagaatgac accactcttt tttactggca
    61741 cagccgttaa caccggtaaa tcggtttcat tcaccacaag tggcgtattg atcgtaaaac
    61801 tctgccctgc ctgtttgagc tgaatgccta aaccagtgat attgctgctt actgcagcat
    61861 tatcaaaatc gcttggtgtg ccactccagc tcaaggtcat ttccattaat gggtcgcgaa
    61921 ccgccgtatc gcaggtaatg gaatacggca cggtttgctc ataattgctg ccattaatgt
    61981 catcaataag cacatcctga aaagttattt caatggtttg agcgttgcta atcgtgcagt
    62041 taggcggctg cactaaataa ccgtgaaaag agacgtttga gagtgccatc gagcaaaagc
    62101 cccacagcaa aataagcgag atccgtttca ttggtaatcc accacaattg tggcactggc
    62161 gttaaattca gctgccgcta acgttgtacc actctgtttc accggcacag cttccagaac
    62221 gggaactccc gaggaaaaat taaatggcaa ccatgagcca gaggttaagt cgagtattgt
    62281 gtgatcacta cttttctgaa cccgaatacc aaacccagga atacccgtgc tgataactgt
    62341 ttcaccgtta atcacgaccg ttgtcgcctg catctgtaga cgcaggtcat ccattgccag
    62401 attgttacaa acaagcgagt agtcaatggg acgtttataa tcaaccctat tgatcttatt
    62461 aatgaataca ttaccaaact caactgccgc accatttacc gtacaaggag gaggggcatc
    62521 gacaagcacc cgtaaattca tacttactga cttactcgct gcaactgcaa agccgctacc
    62581 aacacaacat atcagtaacc cagttaccgc ccagacagaa caagatcctt tcatactggc
    62641 tctcccggcc tcagcctttg cggctctgct cttcatcgac ctagcaactg ttaccgttgc
    62701 aagtgaaaaa gagcggcatc cgagcgccat agtcgttgac ataggtcagt accggcacgc
    62761 tacccatctt cacattcagc ggtgcagagg ttttcggcga catcactatt ggcgagaaac
    62821 cggctgccgg attgccattt ttctgtgtgc tggcattact gaaaatgacg taatacgacg
    62881 tcgggttatt gacggtaaat tcctgaccgt tgcgggttaa agtaatctta tattgctagg
    62941 ggtttttcat gctgacgttc tccagcgcct tcggtcgcca gaacaactta atacgcgttt
    63001 gtagtgctat ttgcagcgtg ttggctttgt ttgattttgg cgggatctca cgcacgttga
    63061 agtagaacag gctttcacga tcggcaggca atttgttgat atccggcatt ccctgcactt
    63121 ttacctgccc gttcatcatt gaatcgatgc gttgcaccgg tggcagaaca gttaacggtg
    63181 aactgatctt gttacccttt tcatcttcga tccagctttg ggccagataa ggcagtttcg
    63241 gatcgttatt gcgcaacgtc acgctgattg atttgtcgct ttcattaaat atcaaacggg
    63301 tacgatctgg cgttacagaa gcctgggcag acaaactggc agcgctaagc agtaacgcta
    63361 gcgtcgtagc acccaatttg gcagagcaaa gtaaatcaga catggatcat tccttaatca
    63421 cgttattggt tagctgaaac agaaacaggg ggattggtgg gtgtcacctg ttgcgtctgc
    63481 tcctgaatga caggctttat gtcatcagga acaaccggcg cgacgttgcc tttatgctgg
    63541 catggcagta acaagccgtt aaacagatcg gcaggcagcg ggtccggcag gttgatatcg
    63601 caatgcgcaa caccactcca gaacacactc atgtgttcgc caggtttcac tccagccaga
    63661 taaacattgc cgtcatcatc gacaaggccc actgtctgct cgttatcatt ttttacttct
    63721 gcgccaaacg gaggatggct gccgtcttgt aaacgcagca ccgccatcgc tttttgacca
    63781 ctgatgacgg caaatttgcg gtagccaatc gccccttcag ttagcgtggc ttgtaccacc
    63841 gactgggttg cttcggcgtt ttccggcagt ttgttgaggt cgatatacgc ctgattgcgg
    63901 taatagttat tgacgtcaga aacgacggct ttaccaaaca tattggtata aacagcagca
    63961 ccgttacctt ctaccggaac atcggcaacg ccatcggcat caatcaacaa gcgtgtcccg
    64021 cccatattct gggtacggtg aagtgcgcca ccgtgggcag taagcgttgc gccgccctgt
    64081 aacgacaagc ccgcagaggt gtattgccct tcatggtagt tcgcactgag gtcaacctgc
    64141 gccagcgaac catcatggct gtaatagccg tcaacgctgg tgtgtttgtc actggtgccg
    64201 acgttcaact gatagtgagt cgcgtcatcg acacggctga aataaccgac ctgactgctg
    64261 tccgtcccac tgccatagta ccgttatagc taacggtact gttgtcgccc cacggcatac
    64321 tgagcgaaat gtacatgcct ttgtcggcct ggttgtcata ctcgtaacgg tagccagtca
    64381 tcgagatgct gacattacga atactgccca tattgaaata gtgcgagagc atgatgttgt
    64441 agtttgtctg ctcctcgcga tcccagtagg tatggcgggt atagttgaga taaaccgaaa
    64501 cacccgcatc gcggaagttc tggttataag tggcggtgta catctctttg tcgttgcccg
    64561 tgcgtaccat tccgctgtca ctggcatcca gatactcgct catggtcata aagttctctt
    64621 ccgagaagcg atatccagca aaagtaacgc ggctgttgag ctggtcaaag tctttggaat
    64681 aactcacacg gaaggagtta ccgtccagcg aacctttgcc ataagcggtg tctttatcca
    64741 gtttggtgtg cgagtgagta acatcaaacg cgaccgcgcc gaatgtagac aaatcgcgac
    64801 cgacaccaag cgccgcagac tgatagtttt catctcccag tgcgccgcca tataacgacc
    64861 agccgttagc gatcccccag gaagcttcag caccagaaaa aaattcaccc tcgacatggt
    64921 gtccccactc ttgcggacgg cccatcatga tcttatagcg aacctgacct ggacgagtga
    64981 ggtatggcat cgaggcggtg ctgatgtcat attcctgcac ctggccgttc tgttcttcaa
    65041 tgcgaatatg caacgtaccg gagacagaat caccaagatc ctgaatacga aacggtccag
    65101 ccggaacctg cgtttcgtaa atcacacgcc ccatctggct gacggtcact tttgctgtgg
    65161 tgtgtgccac gccggaaatg tctggcgcgt agccgcgcag attgggaggc aacatttgat
    65221 cgtcagtact gacactgcca ccaacatagt taaaaccatc aaaaatatcg gatctgaggt
    65281 aatcctcgcc cagcgccagt ttggctttta atgatggtaa cgcccgcctg gcatagtagc
    65341 gactccactc ccattttttt gagtttcatc gccgctgaat tcatcgtcat cattactgcg
    65401 agtatgttga tagttagtct gccagtcagc acgcatacgc cacggcccta ggttaacccc
    65461 aaccgtcccg ttgccgctga tctcgttact atcatcaccg ccattttctt cgtgccgggt
    65521 ttgtgcgttg atgctgtagt ccgcgacgat cccggagatg ccgtcatccc aacgtgaagg
    65581 cggatccctg tcgggataag tatattcgag gtaagcctgt ggcagtgaaa tgactaatgc
    65641 ggactggctt aaatcagcct taatttccat gccttccagt tgaccggatt tcaggcattt
    65701 agcatcgtgg ctccattgca gatttttcgc cacgtcttct tttaaaccaa actgcgctac
    65761 cagttccggt gtcagacaag catagctttt gctcgcgtca tcttcaccag cataccagta
    65821 aatatcgtac tcttccgcca atggctgttt atttagttga acctgtaaat tgtattttcc
    65881 gggctcaaca tatccctggc tggaaaaacg cttcatatca atttttgtgt cgccttttaa
    65941 ttccagaaaa cgggaatcaa actgaatgtc gtcttcagcc cagacactgc tataactccc
    66001 tgacattgcc agcgcaatgc accacggaag aatccgtaat cgaaaaagag aatggtcagg
    66061 catacacgtt tctatccgtt tttattcttg ttcggtcaaa atacatttaa atcccccata
    66121 gcagtgtcgc tatgggggta tgccttacag gtaagtaatc tggaaggtgg tatttgcttc
    66181 aaaattaccc agatctggcg catcagctgc gccgaccagc caggcgttaa agttcagtgt
    66241 ctgcttcgct ttgcctttgt tggtagaagt gtctttcact attttctggt caatacccat
    66301 gccgcttttg taagaggtac ccagagagtc accgatcgcc aggctaacat tgttaaatgc
    66361 cgcaccggta tcggtattga aaatggtgta ataattgccg ctatttgcag aagaaacggt
    66421 accagtaaag gtagtggtca ttgtttcctg agtatcaaat acgcaatctt gcagacgaat
    66481 ctggaaactt ttcggcgtgg tggtgccgtt atttttcagt ttttcggcac cgatatcacc
    66541 catatcaact tccagtttgt gatcgtccgg aacgatagaa cacactgagt cttcaatgg
//
LOCUS       DAJTUP010000007        66525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTUP010000007 DAJTUP010000000
VERSION     DAJTUP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.007"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(22..2469)
                     /gene="glgP"
                     /locus_tag="OO468_000465"
     CDS             complement(22..2469)
                     /gene="glgP"
                     /locus_tag="OO468_000465"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR6257709.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2488..3921)
                     /gene="glgA"
                     /locus_tag="OO468_000466"
     CDS             complement(2488..3921)
                     /gene="glgA"
                     /locus_tag="OO468_000466"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR6257710.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3921..5216)
                     /gene="glgC"
                     /locus_tag="OO468_000467"
     CDS             complement(3921..5216)
                     /gene="glgC"
                     /locus_tag="OO468_000467"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR6257711.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5234..7207)
                     /gene="glgX"
                     /locus_tag="OO468_000468"
     CDS             complement(5234..7207)
                     /gene="glgX"
                     /locus_tag="OO468_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR6257712.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7204..9390)
                     /gene="glgB"
                     /locus_tag="OO468_000469"
     CDS             complement(7204..9390)
                     /gene="glgB"
                     /locus_tag="OO468_000469"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR6257713.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9663..10766)
                     /gene="asd"
                     /locus_tag="OO468_000470"
     CDS             complement(9663..10766)
                     /gene="asd"
                     /locus_tag="OO468_000470"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR6257714.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10958..11551
                     /gene="yhgN"
                     /locus_tag="OO468_000471"
     CDS             10958..11551
                     /gene="yhgN"
                     /locus_tag="OO468_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR6257715.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11608..12948)
                     /gene="gntU"
                     /locus_tag="OO468_000472"
     CDS             complement(11608..12948)
                     /gene="gntU"
                     /locus_tag="OO468_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6257716.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12952..13479)
                     /gene="gntK"
                     /locus_tag="OO468_000473"
     CDS             complement(12952..13479)
                     /gene="gntK"
                     /locus_tag="OO468_000473"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR6257717.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13618..14613)
                     /gene="gntR"
                     /locus_tag="OO468_000474"
     CDS             complement(13618..14613)
                     /gene="gntR"
                     /locus_tag="OO468_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR6257718.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14837..15532)
                     /gene="yhhW"
                     /locus_tag="OO468_000475"
     CDS             complement(14837..15532)
                     /gene="yhhW"
                     /locus_tag="OO468_000475"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR6257719.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15655..16692)
                     /locus_tag="OO468_000476"
     CDS             complement(15655..16692)
                     /locus_tag="OO468_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6257720.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17025..17513
                     /gene="yhhY"
                     /locus_tag="OO468_000477"
     CDS             17025..17513
                     /gene="yhhY"
                     /locus_tag="OO468_000477"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6257721.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17572..19314)
                     /gene="ggt"
                     /locus_tag="OO468_000478"
     CDS             complement(17572..19314)
                     /gene="ggt"
                     /locus_tag="OO468_000478"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR6257722.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19434..19874
                     /locus_tag="OO468_000479"
     CDS             19434..19874
                     /locus_tag="OO468_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR6257723.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19861..20604)
                     /gene="ugpQ"
                     /locus_tag="OO468_000480"
     CDS             complement(19861..20604)
                     /gene="ugpQ"
                     /locus_tag="OO468_000480"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6257724.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20601..21591)
                     /locus_tag="OO468_000481"
                     /pseudo
     CDS             complement(20601..21591)
                     /locus_tag="OO468_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21593..22438)
                     /gene="ugpE"
                     /locus_tag="OO468_000482"
     CDS             complement(21593..22438)
                     /gene="ugpE"
                     /locus_tag="OO468_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR6257725.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22435..23322)
                     /gene="ugpA"
                     /locus_tag="OO468_000483"
     CDS             complement(22435..23322)
                     /gene="ugpA"
                     /locus_tag="OO468_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR6257726.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23420..24736)
                     /gene="ugpB"
                     /locus_tag="OO468_000484"
     CDS             complement(23420..24736)
                     /gene="ugpB"
                     /locus_tag="OO468_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR6257727.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25133..25846)
                     /gene="livF"
                     /locus_tag="OO468_000485"
     CDS             complement(25133..25846)
                     /gene="livF"
                     /locus_tag="OO468_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR6257728.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25848..26615)
                     /gene="livG"
                     /locus_tag="OO468_000486"
     CDS             complement(25848..26615)
                     /gene="livG"
                     /locus_tag="OO468_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR6257729.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26612..27889)
                     /gene="livM"
                     /locus_tag="OO468_000487"
     CDS             complement(26612..27889)
                     /gene="livM"
                     /locus_tag="OO468_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR6257730.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27886..28812)
                     /gene="livH"
                     /locus_tag="OO468_000488"
     CDS             complement(27886..28812)
                     /gene="livH"
                     /locus_tag="OO468_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR6257731.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28860..29969)
                     /gene="livK"
                     /locus_tag="OO468_000489"
     CDS             complement(28860..29969)
                     /gene="livK"
                     /locus_tag="OO468_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR6257732.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30393..30776
                     /gene="panM"
                     /locus_tag="OO468_000490"
     CDS             30393..30776
                     /gene="panM"
                     /locus_tag="OO468_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR6257733.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30975..32078)
                     /gene="livJ"
                     /locus_tag="OO468_000491"
     CDS             complement(30975..32078)
                     /gene="livJ"
                     /locus_tag="OO468_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR6257734.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32349..33203)
                     /gene="rpoH"
                     /locus_tag="OO468_000492"
     CDS             complement(32349..33203)
                     /gene="rpoH"
                     /locus_tag="OO468_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR6257735.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33448..34506)
                     /gene="ftsX"
                     /locus_tag="OO468_000493"
     CDS             complement(33448..34506)
                     /gene="ftsX"
                     /locus_tag="OO468_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR6257736.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34499..35167)
                     /gene="ftsE"
                     /locus_tag="OO468_000494"
     CDS             complement(34499..35167)
                     /gene="ftsE"
                     /locus_tag="OO468_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR6257737.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35170..36666)
                     /gene="ftsY"
                     /locus_tag="OO468_000495"
     CDS             complement(35170..36666)
                     /gene="ftsY"
                     /locus_tag="OO468_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR6257738.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36816..37412
                     /gene="rsmD"
                     /locus_tag="OO468_000496"
     CDS             36816..37412
                     /gene="rsmD"
                     /locus_tag="OO468_000496"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR6257739.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37402..37671
                     /locus_tag="OO468_000497"
     CDS             37402..37671
                     /locus_tag="OO468_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR6257740.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37674..38033)
                     /locus_tag="OO468_000498"
     CDS             complement(37674..38033)
                     /locus_tag="OO468_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR6257741.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38174..38800
                     /locus_tag="OO468_000499"
     CDS             38174..38800
                     /locus_tag="OO468_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR6257742.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38874..41072
                     /gene="zntA"
                     /locus_tag="OO468_000500"
     CDS             38874..41072
                     /gene="zntA"
                     /locus_tag="OO468_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR6257743.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41174..41419)
                     /gene="tusA"
                     /locus_tag="OO468_000501"
     CDS             complement(41174..41419)
                     /gene="tusA"
                     /locus_tag="OO468_000501"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR6257744.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41640..42305
                     /locus_tag="OO468_000502"
     CDS             41640..42305
                     /locus_tag="OO468_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR6257745.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42378..42935
                     /gene="dcrB"
                     /locus_tag="OO468_000503"
     CDS             42378..42935
                     /gene="dcrB"
                     /locus_tag="OO468_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR6257746.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42939..44156)
                     /locus_tag="OO468_000504"
     CDS             complement(42939..44156)
                     /locus_tag="OO468_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6257747.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44290..45339
                     /locus_tag="OO468_000505"
     CDS             44290..45339
                     /locus_tag="OO468_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR6257748.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45394..45981
                     /gene="acpT"
                     /locus_tag="OO468_000506"
     CDS             45394..45981
                     /gene="acpT"
                     /locus_tag="OO468_000506"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR6257749.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46092..47666
                     /gene="nikA"
                     /locus_tag="OO468_000507"
     CDS             46092..47666
                     /gene="nikA"
                     /locus_tag="OO468_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR6257750.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47666..48610
                     /gene="nikB"
                     /locus_tag="OO468_000508"
     CDS             47666..48610
                     /gene="nikB"
                     /locus_tag="OO468_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR6257751.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48607..49440
                     /gene="nikC"
                     /locus_tag="OO468_000509"
     CDS             48607..49440
                     /gene="nikC"
                     /locus_tag="OO468_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR6257752.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49440..50204
                     /gene="nikD"
                     /locus_tag="OO468_000510"
     CDS             49440..50204
                     /gene="nikD"
                     /locus_tag="OO468_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR6257753.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50201..51007
                     /gene="nikE"
                     /locus_tag="OO468_000511"
     CDS             50201..51007
                     /gene="nikE"
                     /locus_tag="OO468_000511"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR6257754.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51013..51414
                     /gene="nikR"
                     /locus_tag="OO468_000512"
     CDS             51013..51414
                     /gene="nikR"
                     /locus_tag="OO468_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR6257755.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51534..51893)
                     /locus_tag="OO468_000513"
     CDS             complement(51534..51893)
                     /locus_tag="OO468_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR6257756.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51890..52165)
                     /locus_tag="OO468_000514"
     CDS             complement(51890..52165)
                     /locus_tag="OO468_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR6257757.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52224..53348)
                     /locus_tag="OO468_000515"
     CDS             complement(52224..53348)
                     /locus_tag="OO468_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6257758.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53348..56083)
                     /gene="rbbA"
                     /locus_tag="OO468_000516"
     CDS             complement(53348..56083)
                     /gene="rbbA"
                     /locus_tag="OO468_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR6257759.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIQARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56080..57147)
                     /locus_tag="OO468_000517"
     CDS             complement(56080..57147)
                     /locus_tag="OO468_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6257760.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57513..59135)
                     /locus_tag="OO468_000518"
     CDS             complement(57513..59135)
                     /locus_tag="OO468_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6257761.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59397..60986)
                     /locus_tag="OO468_000519"
     CDS             complement(59397..60986)
                     /locus_tag="OO468_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR6257762.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61371..62402
                     /locus_tag="OO468_000520"
                     /pseudo
     CDS             61371..62402
                     /locus_tag="OO468_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62489..62965)
                     /locus_tag="OO468_000521"
     CDS             complement(62489..62965)
                     /locus_tag="OO468_000521"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257763.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62958..63722)
                     /locus_tag="OO468_000522"
     CDS             complement(62958..63722)
                     /locus_tag="OO468_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR6257764.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63968..64729
                     /locus_tag="OO468_000523"
                     /pseudo
     CDS             63968..64729
                     /locus_tag="OO468_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64836..65336)
                     /locus_tag="OO468_000524"
     CDS             complement(64836..65336)
                     /locus_tag="OO468_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257765.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65487..>66525)
                     /locus_tag="OO468_000525"
     CDS             complement(65487..>66525)
                     /locus_tag="OO468_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6257766.1"
                     /translation="TISLSQPLGNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGI
                     TFSINWAKNDLLTSHKWKVDRKLSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVS
                     LSGQNSGGVWWDVATNITNAHQSQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGA
                     NLSGGILITRDGLTFGQNVDGTLALIEAPGATGVNVNGWPGLSTDFRGYAIQPVQPYR
                     RDDVILDEKTIGKNYDLPQTSQLVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPF
                     GAVISYSKDTENMAGIVGEDGIAYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSA
                     SGLYQIAATCL"
BASE COUNT        16170 a        17859 c        16321 g        16175 t
ORIGIN      
        1 ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga tatgatcagc
       61 gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc
      121 ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct gacaatcgac
      181 atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca gcgaatcaac
      241 cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag
      301 cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg
      361 cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag caccgacatg
      421 atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac cgttaagcgc
      481 aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct gttcagacag
      541 atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga tgaacacgac
      601 tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa
      661 atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa aaatattcac
      721 gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg taatcacatg
      781 caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta catcgaacaa
      841 cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca gacgcttttt
      901 gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac agtgttgttg
      961 cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt cgtccagcac
     1021 ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac cgttggtgac
     1081 gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt gcaccatcag
     1141 attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg ccagccaggc
     1201 catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt
     1261 atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat
     1321 ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt ccagcgcctc
     1381 gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa acgcgtcgtc
     1441 ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagtaccgg
     1501 atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg ttttatgcaa
     1561 ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt attcctgacg
     1621 caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc gagatacgtt
     1681 ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga atttaccgag
     1741 gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg cgtcggtgtc
     1801 gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt caatccagcg
     1861 cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc gcgtgttgtg
     1921 gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact ctttctggct
     1981 accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc
     2041 cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc ccaggccacc
     2101 gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta accccatcgc
     2161 ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggagagcgt
     2221 acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg
     2281 ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc
     2341 gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa acatcagctt
     2401 gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg
     2461 agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga
     2521 cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca
     2581 cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca gcgaccaggc
     2641 attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca
     2701 gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt
     2761 caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc
     2821 cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg
     2881 accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc
     2941 gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca gcaccaggtc
     3001 gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac
     3061 cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt ccaacgtatc
     3121 gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac
     3181 gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat
     3241 accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga ccgccgtaat
     3301 gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga attccagccc
     3361 atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat
     3421 gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc cgcgcgccgc
     3481 cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat caggacgcca
     3541 gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa
     3601 tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata
     3661 gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt
     3721 gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca cgccacggcg
     3781 aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc
     3841 gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga acatctctga
     3901 acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg
     3961 cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg
     4021 ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac gcagcggcgc
     4081 agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag
     4141 aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc
     4201 acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc
     4261 acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac
     4321 atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta agcttccagc
     4381 gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg
     4441 aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag
     4501 tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag atagtcggcg
     4561 tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt
     4621 gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg
     4681 ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct
     4741 ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc
     4801 aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg ggtgaccgca
     4861 tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg
     4921 acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga
     4981 gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt gatgcagtta
     5041 gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc
     5101 ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat
     5161 ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact
     5221 aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacaa
     5281 ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag
     5341 gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga
     5401 gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat
     5461 atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc tccaccaaag
     5521 cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg
     5581 cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg ttattgccat
     5641 gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg gagagcaaca
     5701 acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca agagtaccgc
     5761 ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt
     5821 ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt
     5881 catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat
     5941 cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgtagccaga
     6001 aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc
     6061 ccacctgata accactagga gcgatatccc acggttcagc aattaacttc acctgcgaga
     6121 gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac
     6181 ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat
     6241 aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg agcgtgttgc
     6301 cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttat
     6361 cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca
     6421 cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact
     6481 catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac atcgccaccg
     6541 ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca ctggcaaact
     6601 gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag ttgatcatca
     6661 ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc gggtgcaagt
     6721 acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc gtgcgcggcg
     6781 gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg
     6841 cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaact
     6901 ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc
     6961 cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc gggcgcgcat
     7021 ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag cgatgttcct
     7081 ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag aaaagtgtga
     7141 agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca atggcgagtt
     7201 gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg tcaggcttag
     7261 tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc cattgcctgc
     7321 attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt tacctggctg
     7381 attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg caacgataat
     7441 ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac
     7501 caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt tatggtggcg
     7561 gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc
     7621 ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc
     7681 gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat agtaagcgcg
     7741 caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg attttttacc
     7801 gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagcat
     7861 cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct tcatgtagtc
     7921 cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc
     7981 ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca ccgcaccgga
     8041 aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc
     8101 gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt cacgataaat
     8161 catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag
     8221 cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga tcagcgtgtt
     8281 ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg tgccatcaaa
     8341 ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga gaatcacgtt
     8401 cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag taccaaaacg
     8461 gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga agggatgctc
     8521 gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg
     8581 atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac gccaggaacc
     8641 caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc
     8701 agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg
     8761 cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc
     8821 gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga tgccgctctc
     8881 tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc caaccaccga
     8941 gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc catccatagt
     9001 atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag ataatagcca
     9061 ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca ggttttgctg
     9121 accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacgcc
     9181 gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg
     9241 ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt
     9301 ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt
     9361 aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc
     9421 atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact
     9481 ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattca
     9541 gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc
     9601 gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa
     9661 gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca cagcagctgg
     9721 tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg caggcggcct
     9781 accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc
     9841 cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc ttcgacggtc
     9901 ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc
     9961 ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg
    10021 gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc
    10081 cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta
    10141 cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt
    10201 tcatctgcca catggccata cagatggccc atctgggtta ataactcacg catatgtcgc
    10261 gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac aagatcattg
    10321 gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa
    10381 gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg gtcaagaatg
    10441 atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta accttgccat
    10501 ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca
    10561 atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt gccagtggtt
    10621 ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac agggcgaatg
    10681 gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc
    10741 cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata
    10801 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca aagtcgcaag
    10861 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
    10921 gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
    10981 agttttattg atcctgatta tggatccgct cggaaacctg cctattttca tgtccgtact
    11041 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
    11101 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgcg
    11161 agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca ttaagatgat
    11221 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc catttatcgt
    11281 gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga tgttgttgtc
    11341 tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg
    11401 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
    11461 gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc agatgttcct
    11521 cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agagcccggt
    11581 agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa tcatcccaac
    11641 gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
    11701 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa cgtggctgac
    11761 aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac cagagtagtt
    11821 cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta cggtggcagc
    11881 cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa tcggcaggcc
    11941 catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
    12001 gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
    12061 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
    12121 taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta
    12181 agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag
    12241 cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt cgccgagatg
    12301 cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga agttacccca
    12361 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
    12421 gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag gcaccaggaa
    12481 cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
    12541 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa
    12601 cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc ggctgtgacc
    12661 gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat ataagatctt
    12721 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
    12781 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
    12841 ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
    12901 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
    12961 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
    13021 caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
    13081 catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa tcacatcaaa
    13141 atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata
    13201 gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct gcatagcaaa
    13261 cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca gcggttcgcc
    13321 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc
    13381 atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg atacgcccat
    13441 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
    13501 gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga caatatcaga
    13561 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta
    13621 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
    13681 gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt
    13741 cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt ggaaaccggc
    13801 aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg ccgcgccgac
    13861 cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc gccgcgcctg
    13921 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatatgg
    13981 caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
    14041 gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa tggcggtagt
    14101 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc acggcgactg
    14161 gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
    14221 cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca tggattcgag
    14281 acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct gataaccgtg
    14341 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
    14401 aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg gcgcacgatt
    14461 gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
    14521 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
    14581 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
    14641 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
    14701 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg
    14761 gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
    14821 aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
    14881 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg gtcgaggctt
    14941 tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg cgttcagcgg
    15001 caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt tccatatcct
    15061 gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt ttgccctgca
    15121 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca ggcatgatcc
    15181 agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
    15241 ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta ttgcccatgc
    15301 tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    15361 ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
    15421 agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc caggagtcca
    15481 gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc
    15541 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
    15601 tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttaatt
    15661 cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt
    15721 ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg tttgatacaa
    15781 cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
    15841 accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcccggttc
    15901 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
    15961 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    16021 tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt
    16081 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    16141 aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct gcggcagtcc
    16201 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt cgacaatctc
    16261 tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat caaagcgacg
    16321 attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca gctctttcgc
    16381 ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg cttccagcgc
    16441 gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca acttaacatc
    16501 gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt aaatgggagc
    16561 ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc tatccttgcg
    16621 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    16681 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    16741 cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata aattgagaac
    16801 gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag caatgtgagc
    16861 aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa taataatcat
    16921 tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg catttgaaag
    16981 tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt gagatagtaa
    17041 tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc cagccggagg
    17101 tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag gagcgactcg
    17161 ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc gtgggccatc
    17221 tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt ggtatctgtg
    17281 tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag atgattgaaa
    17341 tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc gataacgcgc
    17401 cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt aagaagtacg
    17461 cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa tagagaattt
    17521 gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat attaataccc
    17581 cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac cgtccggccc
    17641 aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct gaccttttgc
    17701 ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct cgtccggcaa
    17761 ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc cataatcgat
    17821 gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg ggctaccggt
    17881 aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg acagcgggcg
    17941 tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt tcggtacgcc
    18001 cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct cacccgcgac
    18061 aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt taccgtcttt
    18121 atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct tgccagggcg
    18181 aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg atttggcata
    18241 ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc caagatattc
    18301 cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg catcggcgct
    18361 gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt gtacgatatg
    18421 gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat aatcgccgct
    18481 tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga tcaagccacc
    18541 gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga attcgtccgg
    18601 gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca gcgtgtcgcc
    18661 ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt gattcggcaa
    18721 cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga taaaaccatc
    18781 gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc cgtatttatc
    18841 cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat gcgaagtgag
    18901 tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg tcgctttggc
    18961 gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc ggattaacat
    19021 aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg cgtagcccac
    19081 cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc ccacctgagt
    19141 ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat ggaagacatc
    19201 ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc taaaacaact
    19261 tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat
    19321 tctccagaga ttaaggggca accccagcta agcctggtat ataactctga attaatcatc
    19381 gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac
    19441 gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc
    19501 tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga
    19561 tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc
    19621 agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt
    19681 cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga
    19741 tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga
    19801 ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa
    19861 ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac
    19921 gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa ccagaatccg
    19981 taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg
    20041 aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag
    20101 caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc
    20161 aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac
    20221 cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat
    20281 cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa
    20341 cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc
    20401 cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc
    20461 gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac
    20521 gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat
    20581 gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc
    20641 tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc
    20701 agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag
    20761 ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc
    20821 gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg
    20881 gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt
    20941 gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc
    21001 ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg
    21061 cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa
    21121 caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc
    21181 gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc
    21241 ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc
    21301 catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat
    21361 cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc
    21421 ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg
    21481 cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta
    21541 ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct
    21601 cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg
    21661 ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag
    21721 tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca
    21781 acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca
    21841 gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt
    21901 cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa
    21961 acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct
    22021 tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg
    22081 tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa
    22141 atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc
    22201 tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt
    22261 tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg
    22321 cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga
    22381 tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc tcaatcattg
    22441 gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa
    22501 catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg
    22561 gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc
    22621 gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact
    22681 caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc
    22741 gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag
    22801 aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc
    22861 gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc
    22921 gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata
    22981 cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc
    23041 cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt
    23101 cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc
    23161 gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc
    23221 gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata
    23281 aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac
    23341 tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac
    23401 gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt
    23461 tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc
    23521 tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt
    23581 tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt
    23641 ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat
    23701 aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaact
    23761 tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc
    23821 cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc
    23881 cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca
    23941 tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga
    24001 agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt
    24061 tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac
    24121 cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc
    24181 acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg
    24241 gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg
    24301 aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac
    24361 cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt
    24421 catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct
    24481 gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt aaattctgtt
    24541 cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt
    24601 gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg
    24661 tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat
    24721 aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt
    24781 tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt
    24841 tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat
    24901 gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac
    24961 tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat
    25021 tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca
    25081 tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc
    25141 ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca
    25201 ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt
    25261 tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa
    25321 tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg
    25381 gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc
    25441 ccgcccgctg aacacggcgc tcatgcaaac gtggaaacag ttcatacacc cactttatgc
    25501 gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg
    25561 tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt
    25621 tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc
    25681 gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga
    25741 gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc
    25801 cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct
    25861 aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt
    25921 ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg
    25981 tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc
    26041 agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc
    26101 ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta
    26161 ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg
    26221 tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc
    26281 ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg
    26341 aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt
    26401 aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg
    26461 gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt
    26521 tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc
    26581 atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt
    26641 ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca
    26701 tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt
    26761 cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc
    26821 cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga
    26881 caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg
    26941 taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt
    27001 cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa
    27061 acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg
    27121 gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac
    27181 ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc
    27241 cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa
    27301 cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg
    27361 ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat
    27421 tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca
    27481 gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca
    27541 gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca
    27601 gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg ggcagaataa
    27661 acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga
    27721 aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga
    27781 ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa
    27841 agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac
    27901 ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa
    27961 tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc
    28021 caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc
    28081 ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg
    28141 accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat
    28201 cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg
    28261 acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa
    28321 ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg
    28381 gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt
    28441 caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag
    28501 gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct
    28561 ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc
    28621 catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc
    28681 gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat
    28741 cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa
    28801 ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat
    28861 cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc
    28921 ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct
    28981 tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc
    29041 agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct
    29101 gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata
    29161 gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca
    29221 ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc
    29281 cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg
    29341 gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg
    29401 gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt
    29461 ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc
    29521 gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata accgcgttgg
    29581 gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata
    29641 tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg
    29701 ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc
    29761 acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga
    29821 cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca
    29881 acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct
    29941 gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt
    30001 gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc
    30061 ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt
    30121 tcagtcgatt aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc
    30181 ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt
    30241 atgctggaca tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt
    30301 aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact
    30361 ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa
    30421 actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct
    30481 ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg
    30541 cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg
    30601 aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc
    30661 ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg
    30721 cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt
    30781 caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg
    30841 cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt
    30901 tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag
    30961 ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg
    31021 ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta
    31081 tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg
    31141 cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc
    31201 tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc
    31261 ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg
    31321 ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc
    31381 aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt
    31441 ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac
    31501 accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg
    31561 tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat
    31621 ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag
    31681 ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct
    31741 tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta
    31801 atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg
    31861 tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca
    31921 accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt
    31981 gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg
    32041 atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg
    32101 gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac
    32161 ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct
    32221 attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga
    32281 ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat
    32341 agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg
    32401 cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg
    32461 tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc
    32521 gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca
    32581 aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg
    32641 tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt
    32701 acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac
    32761 cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg
    32821 gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc
    32881 cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct
    32941 ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca
    33001 taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct
    33061 tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac
    33121 atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct
    33181 aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt
    33241 ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag
    33301 accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga
    33361 catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt
    33421 gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac
    33481 cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca
    33541 ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc
    33601 caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc
    33661 agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac catccgtcgc
    33721 accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct
    33781 gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg
    33841 cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc
    33901 gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg
    33961 aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc
    34021 cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag
    34081 atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc
    34141 gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt
    34201 cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat
    34261 ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc
    34321 gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt
    34381 tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga
    34441 tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt
    34501 attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac
    34561 gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa
    34621 actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt
    34681 cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt
    34741 gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat
    34801 ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg
    34861 ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca
    34921 tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc
    34981 cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg
    35041 tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga
    35101 acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc
    35161 gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg
    35221 acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc
    35281 agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc
    35341 ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct
    35401 ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg
    35461 gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc
    35521 ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga
    35581 ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac
    35641 ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt
    35701 accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt
    35761 gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac
    35821 tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg
    35881 aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac
    35941 gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt
    36001 tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct
    36061 gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc
    36121 tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc
    36181 ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc
    36241 ttctgccgct tcgacaatct ctacggtttc cgcttcagcc tgccactctt ctgacgaaac
    36301 ctcttcggcg ttgacgtctt ccggcaacgg cagctcttca cgttcgatag ccactggctc
    36361 cggcgtttct tctacgaccg gttccggctg tgcaacaact tccgcttcag gctgcgcttt
    36421 ttcactttca acaacctgtt cagtgacttc cacaacgtcg gcagcaaaag tttccgcctc
    36481 ggcttcagta tgcgcctgcg gctgctcttc aacggcttgt tcagaagcct tcgcaggctc
    36541 ttgcgcctga acgatttctt ctacaaccgg ttgttcattc tgaacttctg tctctttttc
    36601 cggggtctgc tctttttgac caaagcccag ccaggaaaaa aagccacgtc ttttttcttt
    36661 cgccatttgc gactacactc ctcgctgttg cttcatggca cagcgttaac gctatgtaca
    36721 tagcagctaa aaaaatgatg aaatagtcta tcacttaact taattcacat caccgcctgc
    36781 aagtatgtgt tatctggcgg attgagcaat ttatcatgaa aaaaccgaat cattccggca
    36841 gcggccaaat ccgcattatt ggcgggcagt ggcgaggccg taaactcccg gttcctgata
    36901 gcccaggtct gcgccccacc accgaccgcg tacgcgaaac gttgtttaac tggctggctc
    36961 cggtcattgt tgacgcccaa tgtctggatt gcttcgccgg gagcggcgcg ctggggctgg
    37021 aagcgttatc gcgctacgct gcgggggcaa cgttgattga gatggatcgc gtggtttctc
    37081 agcagttaat taagaatctg gcgacactaa aagcaggcaa tgcacgcgtg gtgaacagca
    37141 acgcaatgtc attcctggcg caaaaaggca caccgcataa tatcgtgttt gtcgatccac
    37201 cgttccgccg tggcttgtta gaagagacga taaatttact ggaagataac ggctggctgg
    37261 ctgacgaagc cctgatttat gtcgaaagcg aagtcgaaaa cggtctgccc actgttccgg
    37321 caaactggtc attacatcgg gaaaaagtgg cgggtcaggt ggcttatcgg ctgtatcaac
    37381 acgaagcaca aggagaaagt gatgctgatt aatattggtc gtttgttaat gctctgcgtt
    37441 tggggatttt taatcctcaa tctggtgcat cccttcccgc gcccgctaaa tattttcgtt
    37501 aacgtggcgc tgatttttac cgtgctgatg catggtatgc agctggcgct attgaaatcc
    37561 actttaccga aagatggccc gcagatgacc accgccgaaa aggtgcggat tttccttttc
    37621 ggcgtgtttg aactgctggt ctggcagaag aaatttaaag ttaaaaaata accttattgt
    37681 tcgccaacaa agctaacaaa gcgcgttcct ttatagctca gcgtaccttt atcgcccact
    37741 gtcagggcgt ggtactgctg ggcgtcgagg cgaaacgtct gctccattcc tccgctttgc
    37801 ggtttgaagc ttgcctcata gcgcatactg gtgcctgccg gagtcacttc ctgctggcgc
    37861 gaacggcgat cgttaatcgg tttttcccgc ttgttgctca ccaccaccag cttttgctgg
    37921 agcggagcca tatcattatc agctttttcc cgtcgctgtt gcataaaacg aaacgatgcg
    37981 gcgacgacaa ttaagccaat gataacaata aagaaaagag gtggtttact catctttatc
    38041 cctcatcgga aaatgcggaa ttaagcatac cctgccagtt atggtgttgt catccgtcca
    38101 ccctcgccac taaactggaa gcaagaccgt aggcattctg cttacgaaaa aataacgaat
    38161 tcaaggaact aagatgcttt ggtcgtttat cgctgtctgt ctttccgcat ggctatctgt
    38221 ggatgcatcg tatcgtgggc caacctggca acgctgggtg tttaaaccgt taacccttct
    38281 tctcctgctg ttactggcct ggcaagcacc gatgttcgac gccattagct atctggtgct
    38341 ggcgggactg tgcgcctcac tgctgggcga tgcgctaacc ctgttgccac gtcaacgtct
    38401 gatgtacgcc atcggcgcgt ttttcctctc gcacctgctg tacaccatct atttcgccag
    38461 tcagatgacg ctctctttct tctggcctct gccactggtg ttgctggttc tgggtgcgct
    38521 gctactggcg attatctgga cgcggctgga agagtaccgt tggcctatct gcacgtttat
    38581 cggcatgacg ctggtgatgg tgtggctggc aggtgaactg tggttcttcc gtccgaccgc
    38641 tccggcgctc tctgcgtttg ttggcgcttc gttgctgttt atcagtaact ttgtctggct
    38701 ggggagccac tatcgccgac gcttccgtgc ggataacgcg attgctgcgg cctgctactt
    38761 tgccggtcac ttcctcatcg tccgctcact gtatctctga taaaacttga ctctggagtc
    38821 gactccagag tgtatccttc ggttaatgag aaaaaactta accggaggat gccatgtcga
    38881 cacctgacaa tcacggcaag aaagcccctc aatttgctgc gttcaaaccg ctaaccacgg
    38941 tacagaacgc caacgactgt tgctgcgacg gcgcatgctc cagctcgcca acgctctctg
    39001 aaaacgtctc cggcacccgc tatagctgga aagtcagcgg catggactgc gccgcctgtg
    39061 cgcgcaaggt agaaaatgcc gtgcgccagc ttgcaggcgt gaatcaggtg caggtgttgt
    39121 tcgccaccga aaaactggtg gttgatgccg acaatgacat ccgtgcccag gttgaatctg
    39181 cggtgcaaaa agcgggctat tccctgcgcg atgagcaggc cgccgacgag ccacaagcat
    39241 cgcgcctgaa agagaatctg ccgctgatta cgcttatcgt gatgatggca atcagctggg
    39301 gtctggagca gttcaatcat ccgttcgggc aactggcgtt tatcgcgacc acgctagttg
    39361 ggctgtaccc gattgctcgt caggcattac ggctgatcaa atccggcagc tacttcgcca
    39421 ttgaaacctt aatgagcgta gccgctattg gtgcactgtt tattggcgca acggctgaag
    39481 ctgcgatggt gttgctgctg tttttgattg gtgaacgact ggaaggctgg gccgccagcc
    39541 gcgcgcgtca gggcgttagc gcgttaatgg cgctgaaacc agaaaccgcc acgcgcctgc
    39601 gtaacggtga gcgggaagag gtggcgatta acagcctgcg gcctggcgat gtgattgaag
    39661 tcgccgcagg tgggcgtttg cctgccgacg gtaaactgct ctcaccgttt gccagttttg
    39721 atgaaagcgc cctaaccgga gaatccattc cggtggagcg cgcgacgggc gataaagttc
    39781 ctgcaggagc caccagcgta gaccgtctgg taacgctgga agtgctgtca gaaccgggtg
    39841 ccagcgccat tgaccggatt ctgaaactga tcgaagaagc cgaagagcgc cgcgccccca
    39901 ttgagcggtt tatcgaccgt ttcagccgta tttatacgcc agcgattatg gccgtcgccc
    39961 tgctggtaac gctagtgccg ccgctgctgt ttgccgccag ctggcaggag tggatttata
    40021 aaggtctgac gctgctgctg attggttgcc cgtgtgcgtt agttatctca acgcctgcgg
    40081 cgataacctc cgggctggca gcagcagcgc gtcgtggggc gttaattaaa ggcggtgcgg
    40141 cgctggaaca actaggtcgt gttacccaag tggcgtttga taaaaccggt acgctgaccg
    40201 tcggtaaacc gcgcgttacc gcgattcatc cggcaacggg tattagtgaa tctgaactgc
    40261 tgacactggc ggcggcggtc gagcaaggcg cgacgcatcc actggcgcaa gccatcgtac
    40321 gcgaagcaca ggttgctgaa ctcgccattc ccaccgccga atcacagcgg gcactggtcg
    40381 ggtctggcat tgaagcgcag gttaatggtg agcgcgtgtt gatatgcgct gccgggaaac
    40441 atcccgctga tgcatttgct ggtttgatta atgaactgga aagcgccggg caaacggttg
    40501 tgctggtagt acgtaatgat gacgtgctgg gtatcattgc attgcaggat accctgcgcg
    40561 ccgacgctac aactgccatc agtgaactga acgcgctggg ggttaaagga gtgatcctca
    40621 ccggcgataa tccacgcgca gcggcggcaa ttgccgggga gctggggctg gagtttaaag
    40681 cgggcctatt gccggaagat aaagtcaaag cggtgaccaa gctgaatcaa catgcgcccc
    40741 tggcgatggt cggtgatggt attaacgacg cgccagcgat gaaagctgcc gccatcggga
    40801 ttgctatggg tagcggcaca gacgtggcgc tggaaaccgc cgacgcagca ttaacccata
    40861 accacctgcg cggcctggtg caaatgattg aactggcacg cgccactcac gccaatatcc
    40921 gccagaacat caccattgcg ctgggactga aagggatctt cctcgtcacc acgcttttag
    40981 ggatgaccgg gttgtggctg gcagtgctgg cagatacggg tgcgacggtg ctggtgacgg
    41041 cgaatgcgtt aaggttgttg cgcaggagat aaggcaaacc gatcgcaaca ttgagcgcga
    41101 tcggtcccct cgcccctctg gggagagggt tagggggtga ggaaaaggcg gcatcgaagc
    41161 caatcagccc ctatcaaccg cctttacgaa tcaaataacg ataaggcagt ccatccgtct
    41221 ctttcgcaac cagttcgtgt tccataaagg tacaaaaccc aggaatatcg cgggtagtgg
    41281 ccggatcgtc ggcgataatc agcaacgttt cgccaggctg catattgcgc acggttttgc
    41341 gcaccatcat caccggttcc gggcagcgca ggccaagcgc gtcgagtgtg tggtcagggc
    41401 tggaaaagag atcggtcatt ttcttctcat cacttaaaaa aacggcgcta gtttacgccc
    41461 tgtgagtccg taagcaaacc aggttaacga ttgcgtgaaa attaaccatt gcattgtcaa
    41521 cgtaaagcag tatcatgcgg cggctcgaaa aaagggtaag cacgttatta tgttaaggta
    41581 acagacgtgt cgtacgtatt gggttccctc accccaatgg ttaatcaaaa aggtacaata
    41641 tgaacgtttt ctcgcaaact caacgctata aggcgttgtt ctggttatcg ttatttcatc
    41701 ttctggtgat cacctccagt aactatctgg ttcagcttcc cgtctccatt tttggtttcc
    41761 ataccacctg gggcgcgttt agctttccgt ttatttttct tgctaccgac ctgaccgtgc
    41821 gtatttttgg cgcaccgctg gcccgacgca ttatcttcgc ggtaatgatc cctgcgttat
    41881 taatctccta cgtcatctcg tcgctattct atatgggttc ctggcaggga ttcggcgcac
    41941 tcgcccactt caacctgttt gtcgcccgta tcgccaccgc cagtttcatg gcctacgcgc
    42001 tggggcaaat cctcgacgtg cacgttttta accgcctgcg tcagagtcgc cgctggtggc
    42061 tggcaccgac agcgtccaca ctgttcggta acgtcagcga cacgctggcc tttttcttca
    42121 ttgccttctg gcgtagcccg gatgccttta tggctgaaca ctggatggaa atcgcgctgg
    42181 tcgattactg tttcaaagtg ttaatcagta tcgttttctt cctgccaatg tatggcgtat
    42241 tactcaatat gctgttgaaa agactggcag ataaatccga aatcaacgct ttgcaggcga
    42301 gttaaaggtt cgttatcaga gttgtgataa gatggatgaa tgagccgtta tggccgttta
    42361 tcgaaaggaa gaagtcaatg cgcaatctgg ttaaatatgt cggaattggc ctgctggtta
    42421 tggggcttgc ggcctgtgat gataaagaca ctaacgctac ggcgcagggt tcggttgcgg
    42481 aaagtaacgc taccgggaat cccgtcaacc tgcttgatgg caagttaagt ttctcgctgc
    42541 cagcggatat gaccgaccag agcggtaagc tgggaacgca ggccaataac atgcatgtct
    42601 ggtccgacgc caccgggcag aaagcagtca tcgtcatcat gggcgatgat ccgaaagaag
    42661 atctggcggt gttggcgaag cgtctggaag atcagcaacg tagccgcgat ccgcagctgc
    42721 aagtggtaac caataaagcc attgagctga aaggtcacaa aatgcagcag ttagacagta
    42781 ttatctccgc gaaaggccag acggcgtact cttccgttat tctgggtaac gtgggtaatc
    42841 aactgctgac catgcaaatt acgctgccag ctgacgatca gcaaaaagcg caaaccaccg
    42901 cagaaaacat cattaatacg ctggttattc agtaagtttt aagatgatga ggcggcctca
    42961 gggacgtgtt ccggaggccg tttttttaat cgccacgtca gtaataacgc gattgcgacc
    43021 agtcccgccg ccgccagata aatcaccggt acgcccgccc agctcatcac cagcccagcc
    43081 agtggtccag tcacgccaag cgataaatcc ataaatacgg tgtaagttgc cagcgccgcc
    43141 ccctgatttt gctgcggaac cgcttttacc gccactacac ccaatgccgg gaacaccagc
    43201 gaaaacccgg cccccgccag taagacgccg attttcgcca tccacggcat agtcgccacg
    43261 ccaaccagta gcaggccgat tatctcaacg ctaaagcaaa tcatcgctac gtttaagcca
    43321 ccgatacggt taatgccgtt agggaataac aaacgcgtac cgacaaacgc acagctaaac
    43381 agcgtcagcg cgaaagccgc accgtcccaa cctttagcgt cataaaacag cgtgataaag
    43441 gtggcgatga cgccaaatcc ggcggaagcc agtgccagcg ccataccgta cagccagacg
    43501 cgcccaagca ccgcgcgaaa cggcagcggt ttgcctttac tggcttttac cgtcggacgc
    43561 gggatcgcca acaaaatggc caccagcgcc acgcccataa tgattaacgc taacgcctgc
    43621 aagccgcccc agtgataaaa cacgacgcct aacggcgcac ccatcgccat cgccccgtaa
    43681 gtgacaatgc cgttccacga aatcacccgc ccgatatgca gcgagccaac cactccaacg
    43741 ccccataggg tcgatcccgt tccggcaaaa ctttgcccaa tcccaaggat gacgcgcccc
    43801 aggcaaagta ataacaggct gatgacaggc agactggcgg ttaatcctgc cgtcagatac
    43861 cccagaccgc tcaaaaagca gccgcataaa ccgaagacga caatcttttt gggtcccagc
    43921 aaatcggcgt aacgtccggc atgagggcgg ctcagcaagg tggcgaaata ttgcaggctg
    43981 ataaccaatc ctgcccagaa ggcgctaaag cccatcacat catggacata gcccggtaat
    44041 acagcgagcg gcaacccgat ggtgaggtag ctggcgaagt taaacatgac aatggagaca
    44101 atgcgcaaat tcaggcgcaa tccgtttagc gcgggttcgg ctacgggttc gggcatgagg
    44161 atcaccacat ttttacaaca gtgtttcatt tttaccatgt gctgacgtga aaatcagcag
    44221 taagaatcag aatattgctg gcgtgactcc cgcgctacac ttaacacaaa aagtcacaag
    44281 gaagccccaa tggaaacccc tcaacccgat aaaacgggca tgcacattct gctcaagttg
    44341 gcctcgctgg tagtgatcct cgcgggcatt cacgcagcgg cagatatcat tgtgcagctg
    44401 ttactggcgc tgttttttgc catcgtcctc aacccgctcg ttacctggtt tattcgtcgg
    44461 ggagtacaac gccccgttgc cattacgatt gtagtggtgg tgatgctgat cgcactaacc
    44521 gcgctggtcg gcgtactggc ggcatcgttt aacgaattta tctctatgct gccgaagttt
    44581 aataaggagc tgacgcgcaa actttttaaa ttgcaggaga tgttgccttt tcttaatttg
    44641 catatgtcgc cggagcgaat gctgcagcgg atggactcgg aaaaagtggt taccttcacc
    44701 acagcgctaa tgaccgggct ttccggggca atggcgagcg tgcttttgct ggtgatgacc
    44761 gtagttttta tgctgtttga agtgcgccac gtcccttaca aaatgcgttt tgcgctgaat
    44821 aacccacaga ttcacatcgc aggattacac cgcgcactta aaggcgtttc gcattatctg
    44881 gcgttaaaga cgctactcag tttatggaca ggtgtcatcg tctggctggg gctggcgcta
    44941 atgggcgtac agtttgcgct gatgtgggca gtactggcgt ttttgctcaa ctacgtgccc
    45001 aatatcggcg cggtaatttc cgccgtaccg ccaatgattc aggtgctgct gtttaatggt
    45061 gtttacgaat gtattctggt cggcgcattg tttttagtgg tccatatggt catcggcaat
    45121 attttagaac cacggatgat gggccatcgc ctggggatgt ccaccatggt ggtatttctt
    45181 tcattgttaa tctggggatg gctgctcggc ccggtaggga tgctactttc ggtgccatta
    45241 accagcgtgt gtaaaatctg gatggaaacg acgaaaggtg gcagcaaact ggcgatttta
    45301 ctggggccgg gcagaccgaa aagtcggtta ccgggatgag gcgacaagtg atacgatacg
    45361 cactttcatt ttccattaaa cgttggccct gatatgtatc ggatagttct ggggaaagtt
    45421 tcgaccttaa gcgcagctcc actgccaccg ggtttacgcg agcaagcacc gcaaggtcca
    45481 cgacgcgaac gctggctggc ggggcgtaca ttgctttcgc acacgctttc cccgctaccg
    45541 gagatcatct atggcgaaca aggcaaacct gcattcgcgc cggaaacgcc gctatggttc
    45601 aacttaagcc atagcggtga cgatatcgct ctgctgttga gcgatgaagg cgaagtcggc
    45661 tgcgatatcg aagtgattcg cccgcgcgtc aactggcgct ggctggcgaa cgccgtgttc
    45721 agcctcgggg aacacgctga aatggacgcc gtgcatcctg atcagcaact ggaaatgttc
    45781 tggcgcatct ggacgcgcaa agaagccatc gttaaacagc gtggcggcag cgcctggcaa
    45841 atcgtcagcg tagatagcac ctatcactcc tcgctgtcag tcagccattg tcagctagaa
    45901 aatttaagcc ttgcgatctg cacccccacc ccctttacgc tcaccgccga cagtgtgcaa
    45961 tggatcgatt cagttaactg atccgcccac ccgactgccc atctattgat ccagaacagg
    46021 taatcagtat gacgaatact taaaatcgtc atacttattt ccgccatcta ttttaatcca
    46081 ttggggttac catgctctcc acactccgcc gcactctatt tgcgctgctg gcttgtgcgt
    46141 cttttatcgt ccatgccgct gcaccagatg aaatcaccac cgcctggccg gtgaatgtcg
    46201 ggccactaaa cccgcacctt tacacgccta accagatgtt cgcccagagc atggtttatg
    46261 aaccattggt gaaatatcag gcagacggtt cggtgatccc gtggctggca aaaagctgga
    46321 ctcattcaga agatggtaaa acctggacct tcaccctgcg tgatgacgtg aaattctcca
    46381 acggtgaacc gttcgatgcc gaggcggcgg cagaaaactt ccgcgcagtg ctcgataacc
    46441 gtcaacgtca cgcctggctg gagctggcaa accagattgt tgatgttaaa gcactcaata
    46501 aaacagagct gcaaattacc ctgaaaagcg cctactatcc tttcctgcaa gaactggccc
    46561 tgccccgtcc tttccgtttt atcgctccct cgcagtttaa aaaccatgaa accatgaacg
    46621 gaattaaagc gccgattggc accggaccgt ggattttgca ggaatcgaaa ctaaatcagt
    46681 acgatgtctt cgtccgtaac gaaaactact ggggcgaaaa gccagcgatt aaaaagatca
    46741 cctttaacgt catcccggac ccgactaccc gcgcggtggc gtttgaaact ggcgatatcg
    46801 acctgctgta cggaaacgaa gggttattac cgctcgatac cttcgcccgc tttagccaga
    46861 atccggctta ccacacccaa ctgtcacagc cgatcgaaac cgtgatgctg gcgctcaata
    46921 ccgccaaagc ccccaccaac gagctggcag tacgtgaagc tcttaattac gcggtaaaca
    46981 aaaaatcgct gattgataac gcgttatatg gcacccagca ggtcgccgac accctgtttg
    47041 ccccttctgt gccttacgct aatctcggcc tgaaaccgcg ccagtacgat ccgcagaaag
    47101 cgaaagagtt gctggaaaaa gccggttgga cgctgcctgc gggcaaagac atccgcgaga
    47161 aaaatggtca gccgctgcgc attgaactgt cgttcatcgg caccgatgcg ttaagcaaat
    47221 cgatggcgga aatcattcag gctgatatgc gccagattgg cgcagatgtc tcgctgattg
    47281 gcgaagaaga gagcagtatc tatgctcgtc agcgcgacgg tcgttttggc atgattttcc
    47341 accgcacctg gggcgcgcca tacgatccac acgccttcct gagttcaatg cgcgtaccgt
    47401 cgcacgctga cttccaggca cagcaaggat tagccgacaa gccgctgatt gataaagaga
    47461 tcggcgaagt gctggcgacc catgacgaaa cgcaacgtca ggcgctgtat cgcgacattc
    47521 tgacccgtct gcatgacgag gcggtttatc tgcctatcag ctacatctcg atgatggtgg
    47581 tatcaaaacc ggagctgggt aatatcccct acgcgccgat cgccaccgaa attccgttcg
    47641 aacagattaa accggtgaaa ccttaatgtt gcgttacgta ttacgccgct ttctgctgct
    47701 gatcccgatg gtgctcgccg cctcggtgat catttttctg atgctgcgcc tcggtaccgg
    47761 cgacccggcg ctcgattatc tgcgtttatc taacctgccg ccgacgccgg agatgctggc
    47821 ctctacccgc accatgctcg ggctggatca gccgctgtac gtccagtacg gtacctggtt
    47881 gtggaaggcg ctgcatcttg actttggtat ctcattcgcc agccaacgcc cggtactgga
    47941 cgatatgctg aacttcctgc ccgccacgct ggaacttgca ggtgcggcgc tggtattaat
    48001 tctgctcact tccgtaccgc tcggtatctg ggcggcgcgc caccgcgatc gtttgccgga
    48061 tttcgccgtg cgctttatcg cgtttcttgg cgtgtcgatg cccaacttct ggctggcatt
    48121 tttgctagtg atggcgtttt cggtgtatct gcaatggcta cccgcgatgg gctacggcag
    48181 ctggcagcac atcattttac ctgcggtttc catcgccttt atgtcgctgg cgattaacgc
    48241 gcgactgctg cgcgccagta tgctggacgt cgccggtcag cgtcacgtca cctgggcgcg
    48301 gctacgcggc ctgaatgaca aacagaccga acgccgccat attctgcgca atgcctcgct
    48361 gccgatgatc accgccgtgg ggatgcacat tggcgaattg attggcggga cgatgattat
    48421 cgaaaacatc tttgcctggc ctggcgtcgg gcgctatgcg gtgtcggcga tttttaaccg
    48481 tgactatccg gtgattcagt gctttacgct gatgatggtg gtggtttttg tggtctgtaa
    48541 tttgattgtc gatttgctca acgccgcgct ggacccgcgc attcgtcgtc atgaaggagc
    48601 gcacgcgtga actttttcct ctcttcccgc tggtcggtac gcctggcgct gatcattatc
    48661 gccctgctgg cgctgattgc gctcaccagc cagtggtggc agccgtacga tccacaggca
    48721 attgatttgc cgtcgtgcct gctttcgccg gatgcgcagc actggctggg caccgatcac
    48781 ttaggtcgcg atattttctc gcggctgatg gcagcgaccc gcgtgtcgct cggttcggta
    48841 atggcctgcc tgctgctggt gctgacatta gggctggtta ttggcggcag cgccgggttg
    48901 attggcgggc acgttgatca ggccaccatg cgcgtcgccg atatgtttat gaccttcccg
    48961 acctcgattc tgtcgttctt tatggttggc gtgctcggca ccgggctgac caacgtaatt
    49021 atcgccatcg ccctgtcgca ctgggcgtgg tatgcacgca tggtgcgcag cttggtgatt
    49081 tcactacgcc aacgcgagtt tgtgctggcg tcacggcttt ccggtgcggg ccatgtgcgg
    49141 gtgtttgtcg atcatctggc aggcgcggtg atcccttcgc tgctggtgct ggcaacgctg
    49201 gatatcggcc atatgatgct gcacgtcgcg gggatgtctt tccttggcct cggtgtgacc
    49261 gcgccgaccg ccgaatgggg cgtgatgatt aacgacgcgc gccagtatat ctggacccag
    49321 ccgctgcaaa tgttctggcc ggggctggcg ctgtttatca gcgtgatggc ctttaacctg
    49381 gtgggtgacg cactgcgcga tcatctggat cctcatctgg tgacggagca cgcacactaa
    49441 tgccgcaaca gattgaacta cgtaatatca cgctacaggc cgcgcagccg ctggtacacg
    49501 gtgtatcgtt aaccctgcaa cgcgggcgcg tgctggcgtt agtcggcggt agcggcagcg
    49561 ggaaatcatt aacctgcgcc gcgacgctgg gcattttgcc cgctggcgtt cgccagacgg
    49621 cgggggaaat tttagccgat ggcaaaccgg tttcgcctta cgccctgcgc ggcatcaaaa
    49681 ttgccaccat catgcagaac ccgcgcagcg cctttaatcc actgcacacc atgcacaccc
    49741 acgcgcggga aacctgcctg gcgttaggga aacccgccga tgacgctacg cttaccgctg
    49801 ccatagaagc ggtggggctg gaaaacgccg cgcgcgtgct gaagctgtac ccgttcgaga
    49861 tgagcggcgg catgttgcag cgcatgatga ttgcgatggc ggtgctgtgt gaatcaccgt
    49921 ttatcatcgc cgatgaaccg accaccgacc tcgacgtggt agcacaggcg cgcatcctcg
    49981 atctgctgga aagcattatg caaaaacaag cgccgggaat gctgctggtc acccatgata
    50041 tgggcgttgt ggcgcgtctg gcggatgacg tggcggtgat gtcacaaggt aaaattgtcg
    50101 aacagggcga tgtagaaacg ctgtttaacg cccccaaaca tacggtgacg cgcagcctgg
    50161 tttccgctca tctcgccctc tacggtatgg agctggcatc atgaccttac ttaacgtctg
    50221 cggcctttcc catcactatg cccacggtgg atttagcgga aaacatcaac atcaggcggt
    50281 gctgaataac gtttccctga ccctgaaaag cggcgaaacc gtcgccctgc tgggacgcag
    50341 cggctgcgga aaaagcaccc tcgcgcggct gctggtaggc ttagagtctc cctcacaagg
    50401 gaatattagc tggcacggcg aatcgctggc gaaacttaat cgcgcccaac gtaaagcctt
    50461 ccgccgtgat attcagatgg tgtttcagga ctccatcagt gccgtgaatc cgcgcaaaac
    50521 cgtgcgcgag atcctgcgtg aaccgatgcg ccacctgcta tcactgaaaa aatccgaaca
    50581 actggcgcgc gccagcgaaa tgctgaaggc ggtcgatctc gatgacagcg ttctcgacaa
    50641 acgcccaccg cagttaagcg gcggccagct ccagctcgtc tgcctggcac gcgcgctggc
    50701 ggtcgaaccg aaattgctga ttctggatga agccgtttct aaccttgatc tcgtgttaca
    50761 ggcgggcgtc attcgcctgc tgaaaaagct acaacaacag tttggcactg cctgcctgtt
    50821 catcactcat gacttacgcc tggtggaacg cttttgccag cgagtgatgg tgatggacaa
    50881 cggacaaatc gccgaaaccc aggcggtggg agataaatta accttttcct ctgacgccgg
    50941 acgtgtgcta caaaacgcgg tattacccgc attccccgtg cgccgtcgca cctcagaaaa
    51001 ggtttaacgc caatgcaacg agtcaccatc acgcttgatg acgatttact ggagacgctg
    51061 gacagcctga gccagcgtcg tggttataac aaccgttccg aagctatccg cgatattctg
    51121 cgtagtgccc tggcgcagga agctacccag cagcacggca cacaaggttt cgcggtgctg
    51181 tcgtatgtgt atgaacacga aaaacgcgac ttagccagcc gtattgtttc cacccagcat
    51241 catcaccacg acctctccgt cgccacgcta catgtgcaca tcaaccacga cgactgtctg
    51301 gaaatcgccg tgttgaaagg tgacatgggt gacgtgcagc attttgccga tgacgttatc
    51361 gcccagcgcg gcgtgcggca cgggcatttg cagtgcctgc cgaaggaaga ttgagttatt
    51421 tctaaaacaa ggaaagaaaa gatattaact gaacttaatt tataaaatta agttcagttg
    51481 catagataga tcctgtaaag gcagatattc tgcgtaatgt acgtcgctaa aatttataaa
    51541 tgattcaaac gctcattcag tgtttcaata atataggtat taacagagac ttgttgctgc
    51601 gcagccgcat tattaaggcg ttcactcagc gattctggat agcgtaacgt aaaggtctta
    51661 atcttttctg tacgcgcata cggctcaata cccgcagctt tacaatcctc aagatactct
    51721 cgcagggaaa gctctccttc tttttgcagc ccctgaatac tgtcagagac aaaatcacaa
    51781 taacctgaca agcccaggaa tttgccacga aatgcattca gttctggcac gtaggtaata
    51841 acagctggtt gcccggcaat ctccatagag tttggcgtct tcagtttgat catggtttta
    51901 cccctatact tagtaaccag tcacacacgc tttctacagc gcctttatcg gtatctggcg
    51961 acggatgagg ccgatggaaa cacgcaacgc tcatatttag tatgaacttg caacgcgaac
    52021 ctcttccttc cttaatttct ccgcctaatg ctttaaccag tgactctata tcagaccact
    52081 tgatcccttg aggaacagga gttttaaata tttgatccaa tgtatttttt tgtttcttcc
    52141 gtaaagcctg taccttggtt ctcatggcat gtccttatga cttcacttta tgacatcaca
    52201 gtatgaagtc aagtttccct tccttacgcc attgtcccaa tcgtcttcct gaatcgcagc
    52261 agtgcgatgg tgaaaaacgc gccgccaatt gccatcagcg tcaggaactg cggccagacg
    52321 atttcgaatc cggcaccccg gtagaggatg gcctgcgcga ggctaacaaa gtgtgtcgtc
    52381 ggcatggtca gcataatgtc ctgcaccatc tgcggcatac tttcgcgcgg cgtggatcca
    52441 ccggaaagca tttgcagcgg cagcagcacc agaatcacca gcagccccag ttgcggcatt
    52501 gaacgcgcta tcgtccccat aaaaatgccg attgacgtgg tggcaaacag actgagcgcc
    52561 acgcccagca taaacagcgg gatcgagcct tcaatcggta cgcccagtac acctttcacc
    52621 atcagcacca gcgataatcc cgataccacc agcaccacca gccccatcga ccagatcttc
    52681 gccatcatga tctcaaacgg cgttatcggc atcaccagta aatgttccac cgtgccgtgt
    52741 tcacgctcgc ggatcagcgc cgatccggtc aatacaatcg ccagcatggt aatgttgttg
    52801 atgatcgcca tcaccccgcc aaaccacgcg ggatcaaggt tcgggttaaa gcgcatccgg
    52861 gtttccagcg ataccaacgg ttcgctgtta tcacggtagc gcgcgacaaa gctgttcact
    52921 tcaccgttga taatattctg gatataccca ttgccggtaa atgcctggct catgcgcgtg
    52981 gcatcgacgt tcacctgaat atccggctgg cgtccggcga ggacatcacg ctgaaaatta
    53041 ggcggaatat ttatcgcgaa ggtatagcgt ccggcgtcca gtccggcatc catctcatcg
    53101 gcggtgatca tctccggtgg caaaaaccac ggacgataga agctgttaac gatccggttc
    53161 gataactgcg attgatccat atcggcaatg gcgatcggcg cgaggttcaa cgatcctggc
    53221 gtaacggtcg ctgacgaata caccgacacc gtaaacgaga agacaatcag cgtcagcatc
    53281 gctttatcac cgagcagact gcgcaactct ttgataccca gattaaaaat attgcgtaaa
    53341 tggcgcatca tccctcctgt tttttcagca gcaggatgct taagcccatc accagcggga
    53401 tggctatcag caacgggata aaaagttgcc acaaatcagt cagatccagc gctttcgaga
    53461 acgttccgcg ggcgatagtc agaaaatgac tggtcgggta aacctcgccg atccaacgtc
    53521 caggcccttc cagcgaagct accggatcga tcatcccgga aaactgtgtc gccgggatca
    53581 atgtgataat cgccgttccg aaaatggcgg caatctggct tttcataaag gtggagatca
    53641 gcagccccat tccagtggca atgatgatat acagcagcgc cgccaaggtg agcgtcagga
    53701 aactgccttt atgcggtacg ccaaacacaa acaccgacag gccgcagagc aggaaaaagt
    53761 tcagcatccc cagcgcgatg tacggcagct gtttgccaag caaaaattcg ctacgggtgg
    53821 tgggggtcac gtaaaggttg ataatcgacc ctagctcttt ttcccgcacc acgctaaggg
    53881 cgcttagcat tgacgggatc atcatcagca gaagcgggat caccgccgga acaatcgctg
    53941 gcaggctttt tacgtccggg ttatagcgat aacgcgtctc aatgttcatc agcccgcttt
    54001 ggctggcggg tgtcgattgt cggctcgcca catcctgaag ccaactctgg tgcattgcct
    54061 gcacgtaacc ttttaccgtt tcggcacgac tgggcatcgc tccgtcgatc cagacgccga
    54121 gttccacagg cgtaccacgc gcgatatcgc gcccgaaatt gggcgggatc tcaatggcca
    54181 ccgtgatatc gcccgcacgc atccgacgat caagctcgtc ataactggtg agcggcggct
    54241 gttcgataaa gtaacgggaa ccggagaggt tgagtgtcca cgcctggcta ctgacggtct
    54301 ggtcgcggtc gagcaccgca aagcgcaggt tttccacatc catactgatg ccgtaaccca
    54361 ttatcagcat caggatcacc gttcccatca gcgccagcgt cgaacgtact ggatcgcgtc
    54421 gcagttccag cgcttcgcgg cggctgtagc taaacagacg gcgcaggcta aatccctgac
    54481 gcggcgcgtg ggtggtgtcg tgtaccacgg gcggcgcttc ggcttcgttg ctctgccctg
    54541 ccgcttcctg caaataggcg ataaatgcct cttccagact ggcggctccg cgtttctcaa
    54601 ccagttcctg cggtgtaccg ctggcaagca cttttccggc gtgcatcagt gagatgcggt
    54661 cgcaacgttc cgcttcgttc ataaagtggg tggagatgaa gatagtcact ttgtcctggc
    54721 gcgagagatc gaccatcaac tgccagaaca tatccctcgc caccggatcg acaccagaag
    54781 taggctcatc gaggattaac atctccgggc gatgaatcac cgccaccgcc agcgaaagcc
    54841 gctggcgaat gccgagcggc aatgactccg gcagaacatc ttcaacgtcg ttgagcttaa
    54901 aacgctcgct catttcagcc actcgcgcgg gaatttccgc ttccgggatg tgaaacaaac
    54961 gggcatgtaa ctccaggttt tgccgcacgg tgagttcgtt atagagcgaa aacgcctgcg
    55021 acatatagcc cacacgacgg cgggtatcga tatcttttgg atcaaccggt tgcccaaata
    55081 gccacgcctc accttcgctg gcgggcagca gtccggtaag cattttcatg gtggtggatt
    55141 taccgcagcc gttcgaacca aggaatccaa aaatctcccc gcgtggaatg cggaaattga
    55201 cgtgatcaac ggcaacgaag gaaccaaaac gcatggtcag atcgcgcgct tcgatggcaa
    55261 tctctgcgtt ttcaggttga tacggtggga tcactaccgc ctgatgcgcc tggcgttgcg
    55321 cttgcggtaa cagattaata aatgcttctt ccagcgtggc gctttgcgtt tgctgccgca
    55381 gctcttcggc gctgccggtt gccagcactt ctccggcatt catcgctacc agccagtcga
    55441 agcgttcggc ctcttccata taggcggtgg cgaccagcac gctcatattg ctctgccgct
    55501 ggcgaatact gtcgatcaga tcccagaact gggcgcggga gagcgggtca acccccgttg
    55561 ttggctcatc aaggatcaac agttccgggt cgtggattaa cgcgcagcac agcccaagtt
    55621 tttgcttcat cccgccggag agtttacctg ccggacgatc gcgaaacggt gctaacccgg
    55681 tgctggtcag cagctcatta attcgcgctt cccgctccgc tttatcgtga ccgaacaggc
    55741 gagcgaaaaa atcgacattt tcatacaccg acaaggtgtg gtagaggttt ttgcccagcc
    55801 cctgcggcat ccaggcgatg cgcgggcaga cgtctcggcg atgcttcggg tcgtgcatat
    55861 cgccacccag caccaccaca ttgccctgct caatgacgcg ggcaccggaa atcagtgaca
    55921 ataaactcga cttcccgacg ccatccggac caatcagccc gaccatgcaa cgggcctgaa
    55981 tatcgagagt gatattgttc agcgcaacgg tttttccata atgctggctc acgcccgcca
    56041 gttgcgcgac aggcgggacg ggaaccagtt ccagatgcgt cattgcggca acctcaccac
    56101 gaggtcgtca ggccacggaa gttcttcatt cacccgcacc cacgctacgc ccggtaaacc
    56161 ggttttgaca tattccagat gctgctggag taattccggt gggatacgcg ctttgacgcg
    56221 gaacatcagt ttcagccgtt catcgctggt ttcgacggtt tttggcgtga actgggcgac
    56281 actggcgaca aaactgatgg ttgcaggaat acgcagatct ggcgcggcat cgaggatcag
    56341 ccgggcttca ccgcccagtt tcagcgtgcc cgcctgttcg gttggcagga agaaggtcat
    56401 atagacgtcg ctgagatcga ccatattcag cacccgaccg cctgccgcca gcacttcccc
    56461 tggctcggca acccgatact gcacgcgtcc gtcacgcggg gctttcagtt cactgtcatc
    56521 gatatctgcg gcaatgcgcc gttcagtggc ttgcgctgct tcgacccgag tttgcgcctg
    56581 aatgatattg gtgcgtgctg cttctatagc cgctttagaa gccgaaacct gggctttcgc
    56641 tgattccagc gcagctcggg cgctctcagc ggcggcgcgg tcgtcatcca gctgttgcgc
    56701 agaaatagcc cctcgttggg cgagcgagcg ggaacgcgta tgacgtttgg caacagagtc
    56761 cagttcggcc tggcgttgat tgaccagcga ctgtgcggca cgagtttcac tttgtcgttg
    56821 ctccagcaaa gcctgcgcgg cggcaacggc gctttgtgcc tctttgattt gcgcgatggc
    56881 ttccagtcgc tgttcctgca acacgcgagt atccatcttc gccagcactt cgccttcgcg
    56941 aacaaactgg ccttctttca ccagaatggt gtcgatacgc ccggcaattt tgctggcgat
    57001 atccacttcc gtcgcttcaa tgcgcccatt gctgacagca aagccttccg gcacacccgc
    57061 cgggcgcaac agccaccacg ccacgacggc agccaccgcc agtgccccga caacccacca
    57121 cgccagatgg cgcttactct tatccataat cgacccgcca taatccctgt aagcaaacga
    57181 cgcgtgttac aactgccgcc gaacgacgtt cagcggattc ttccatccgg gataagaagg
    57241 aaacgacaaa tacgaccagc gcgcagagca ttgagcgcca acggggaagg cttagtgaaa
    57301 acgggtagta atgttgtcat catcagcatt cctggccgta aatgaaacac gcatactcat
    57361 aagtgtgatc tataaaactg gctgacaaaa cggtatccat cctgttggat cgcatttcac
    57421 gcctgttcag ttgcaggaac agagcattcc tttttacgtt ttatcaacag tctcaaccag
    57481 atagtaacat taatattcat gttgtctatg gttcagagct gtaacattgt gcgccttcta
    57541 atcaaaggca taagtccatt tctgtttttt tcatgctggt aagacttcca gggagtggtt
    57601 atctctggcc ctgcaccagg gttaatttgg caattatgca tgctgaacat tcttccagca
    57661 tgtttactgt cacgggcgtt aagacctaaa actgtccccg tttcacctat gcgttctctc
    57721 ccaccatttc cgttatgtgc aacaacttga tttataccta acatttgtga acatgctaat
    57781 agtgcttcaa gtttaggtct gtaatctgtc gaaacagaaa aataacgatt aaaggatcga
    57841 caagagatag cccggctagt tttaaacttt ttgccattaa ttatagcatg atgcttttta
    57901 ttaataattt ccgctaattc gaccggatcg agagcattgg tcatttcatc aatagagaca
    57961 gttatcggtc ctataatgta acgttttcgc tgatcatcaa aagttatccc atgatgattt
    58021 gccattattt tgtatttggg gtcatagaaa catacaggat aatcttttat cccgtcgtag
    58081 gtatcctcat caagtggttt catgcgcgtg aagttttgca tatagttacc attgcaattc
    58141 gtttcatgat ttccagctaa tacatgaaca ttttcattga tactcattat gcgttttaac
    58201 aaatcgatga taaattgatc gccacagata ttactaaatc gatcgccggt ttggtcgcca
    58261 agaaacgtac atggcgtgac tgcgtcttta ttaataacca aataaggctg aagactttct
    58321 aaaacattat ctcttctgtt cagtatactg attattgtat taacattagt gtcaagttgc
    58381 tcattaaagt tctcccttag caatacgttg agaacttcat cttctatatt aagcaactgg
    58441 ctaaggaaat tttctccttc agccatgatt cccaaatgtc ctgttgcaaa aagcgcgtac
    58501 aaaactgcac cgactgagcc atcggtatcg ccaaaatagg tcggcctggt acttgtggga
    58561 tcaacaggtg aacaattgga gaaataggtt ctgtcagcga actggggttt tataattgtt
    58621 tcgaatttaa tatcaaatac attgtattgc gatatttccc agttccagat gggtttgtac
    58681 gactcagtaa ctgcaacatc attagcatta tcatttactg tcatattatt aagtaaataa
    58741 acaatgctgc accaattaat atcggtatta tcaagagggc aaccgtaatt tttactatta
    58801 attgtcacaa taaactgata acagttattc ctacgtagaa tatgagcgcg catgcccgat
    58861 agcatactat atttttctga atgattcgtt tgtatggtaa atgaaatttt gtcatcttga
    58921 atagattgtt ggaaacgttc tatttgccaa tcatcaattt tttccacaac ttcaccgagc
    58981 attgtcctgg caatacttga tatatcatca cctataccat ttgcaatata cccaaacagt
    59041 tctttagtga agttggtaac atgctcttgc accatatcat ttgttgcgat tgtcgttgta
    59101 ctgccattag tgcctgccac agttccaatt ttcatctttt acctcatatt attcaccgcg
    59161 aggaatacat agttaacatt ttatcttatg acatacacct catattcgat ggggaaataa
    59221 ttatattttg cacaaatcat tcagccttaa agcataatat gaaatttctt atctcatagc
    59281 agagaaagtc cctaaatatt gtagaggcgt catcagtaca tacctctaca atcgattaag
    59341 aaaacctatg aaatactggc aaaattaaaa agagatacca cagcactcat gaagttttat
    59401 tgaggttgaa aacaactaag ccctgggaca ccttttttat caccacgttc gttaggtgct
    59461 atcttccaag aatttagccc catggtataa ttatcaattt tttgatgctg gtctacccct
    59521 aatccataat ggccaaattt ctgtttagtt tttttgagcg tctgtccaca tttgaagata
    59581 tcttcaggtt ttggtctgaa agcagtagtg ttattaaaat aattttcata tacaggtgtg
    59641 ggtgctatag cattgcttct aattagatgg agaagatgat catccatgtg ttgtttatgt
    59701 tttttattac atatatttgc taattcctca ggatcaattg ggtctttcat ctgattaaat
    59761 ggaacctgca aagcaccgag acaataacat tgattttctt catttcttat tattccgtga
    59821 tgactggtta aaactttagt ttctgagtta taattacaaa catcgagcgt tttaattaag
    59881 ttatacgtat caccggcaga gagtttataa ttttctagtc gtgcagtata gttaccatta
    59941 aaattgattt catgatttcc agccagaacg atgacatttt tattaatacg gctatcttta
    60001 ttaccctcca tatttcgcat agaatttagc aaagtaagga tatatttatc accaaaaata
    60061 gtactaaatc tatcgcctgt atgatcccca aggaagatac aaggggttat cgcatcgtcc
    60121 tttaaaaaaa catgttgatg caactcattc aatatcatgt ctctcgtatt taataaattg
    60181 tagagcaatt tattattttt atgtatgtat gcagatgaag ccatgtcttc gtgttttaat
    60241 aattcacata ataaactcca acccttttcc ctgatgccta tatgccctga aacgagcaat
    60301 gaataaagta cagcgcctac tcttccatct gtatcgccaa aataagccgt gttggcattt
    60361 tgtgggttaa tcacaccaca ttgtttataa aagtctattt tagaaaacac agatttaaca
    60421 tagctatcaa acttatcctt aggaaacgtc tctttagaga catcccaacg ccagtcagct
    60481 ttaaaattac cgctaagaag agtaaagaaa ttttcaggta tgatagaaag cccattaact
    60541 ttataaatta tgtctgattt atctccggag aaattaataa aataataacc atccttacac
    60601 tcaatcttaa ctgtaatggg agacgataat tttatcacat ggttattgtc tggtataatt
    60661 gtaaagataa acgtgttagt agtattatcg gcaaaactat tatattcttg aatatctata
    60721 gatggctcac tattgtaaaa ccatattttc tccatctgaa gactatgttc atttaccctc
    60781 cagtgaccat gttgcaggaa atagatgata tgcatgccaa aatcgttatc tatttgcgaa
    60841 tcattatcca gcttatcaat ttcaactatc tttgaacgat gtgttttatc aaccttgcta
    60901 tggtgttgat gattattaac tttatctgaa tgcttatctt tatggtttga agggatgtac
    60961 ttaaatatat tgtcaattgc tttcataaat aacccgcgat atttttgacc tgttagtaaa
    61021 aaataaatga tacatgtctg aaagacatat ttacgacgat ataaaacaat aatacttata
    61081 tgttcgctga ttttaagcac cgagactatc cggtctttta ctaaataatg gaaaacaatc
    61141 catccctgga agacaattgt aaaaaaaaac aacagaataa gaattaatct taggataaat
    61201 atttatttat catggccttt tgaccgctaa aaaatggcac tcggcacagt aaaccccgct
    61261 atagttatgc tatgcatttg tctttaatga tatctcgtcg ttatattaac aggctgaaat
    61321 tatcattaat gcacatttca atattagcag ggatacctga ggagtttaaa gtgaacatat
    61381 atatcgggta gcttttcaaa ttaatccctt tgattatagg cttaatttgc atcgcgttag
    61441 gtggctttgt gctggaaagt tcagggcaac gtcacgggtc atgtgctgat ttctctggcg
    61501 gccatatgcc tggcattatt cactaccgca tttattatca tttcgcaact cacgcgcggc
    61561 gttaatacgt tttacaatac attgttcccc attattggct atgcggggtc aattatcacc
    61621 atgatatggg gttgggcact gttagcaggt aatgatgtga tggcagacga gtttgttgcc
    61681 ggccatgtta tttttggcgt tggtatgatt gccgcatgtg tatcgacggt ggcagcgtcg
    61741 tccggtcact ttctgctcat tcccaaaaat gcagcgggga gcaagagcga cggaacaccg
    61801 gtacaggctt attcttcatt aatcggtaat tgcctcattg ccgttcccgt tttactcacc
    61861 ctgctcggtt tcatttggtc tattacgctg ttacatagtg ccgacataac tccgcattat
    61921 gtcgccttgg gttaaccgca atctgtgcct gtctaattgg cctagtggcc acaatcgtcc
    61981 atcaaacacg taatacgttc tcatccaaag agcactggct gtggtgttat tgggttattt
    62041 ttctcggctc agtcacggtg ctgcagggga tatacgtctt agtcagttcc gatgcaagcg
    62101 cccgactggc tcccggcatt attctcattt gcctcggaat gatctgttac agcatattct
    62161 caaaagtctg gctactggca ctggtctgga gacgtacctg ttcgttagcc aacagaatac
    62221 cgatgatacc ggtatttacc tgtctgtttt gcttgttttt gtcatcattc ctcgcggaaa
    62281 tcgcacaaac tgagctggac tactttattc catctcgggt tctggttgga ttaggcgcag
    62341 tatgttttac attgttctct attgtttcaa tattagaggc cggttctgcg aaaaaaaatt
    62401 aaaatttaca taatcccagc gttattcata tcttaacgct ggttttttac gaacttagtg
    62461 aaataatgta gaatataaaa ttataatttt atattggagc acatgtgtta atgatcaacc
    62521 ccgggatact aaaaatatag acattattaa acttgcttag cctcgcattg cctttacctg
    62581 ttaaagatgc atcgagaaaa ttgagtctga agttctcatc ctttatctga ttttccagtc
    62641 ccgaaacttt ggccttagca ttacctgaga tccgtccgtt actatctatt ttgtaggtaa
    62701 aaaaatacga atgggaaaat ttcctcggtg tatcttcatc agttaagcca acaatcgtaa
    62761 aactaccgct accatctttt tttaaaatga ctgaaaggtt agcagagtag tataaagaat
    62821 gatctgtttt agcatttctt gttaacgtcg acgtacattc aagagacgtt gtgctaccat
    62881 cacgagcctt ccagagagca aaaaaacaca cctgtagcaa tgtgaaaaca agtattaata
    62941 taaaatacat tttcttatta gtcatataat agcacctctg aacaaattgc ataagtttcg
    63001 tcattagagg attttataca tcttgataaa taccgctgcc cttccccaag caaataatgc
    63061 atactattta catcatatat aaaatttgca cctggcacac agggtaaatg ctttttcgaa
    63121 atttcagcgg cctctatttg agatttagcg atgccaatct cgttaagatt ctctgaaaga
    63181 tataaatcac aggtgtcaat cttacctgat ttaaccaatg gataatccgt tatactgtag
    63241 gtatctttaa aagccacggc aaacatttca ataataacaa ttactaccac aagcgaaatc
    63301 aaaatgcgaa aaagaggaat actccttttg cgctctactt tttcaacaac atcttcttgt
    63361 aaatgagaat catcataaga ttgtttactt attgttatgt tttcactgat gtaaaagcct
    63421 actttgggta tcgtaagaat cacattttct tcaacaccaa tatttttaaa acttttgcgt
    63481 aacagggata tatattggtt caatgtgttg tttgacagat caaatgaaaa tctctccaaa
    63541 acgctatcaa tgattgagtt cctgtcaacc gctccaccat tactttccag taacactgcc
    63601 aaaatacacc ccgccgtagc tggaaaattt atctcatcct gggtatttac attcgagagc
    63661 gagcatttgt tcggatcaaa aataatcgcg tcattgataa tgaaaaaaca tctccgctcc
    63721 atgcaggcaa caacacaaaa taagattttg tcattttagc ctattcctgg tggaactgac
    63781 acaagaaact aaaatacaaa tataaccctg aaaaagtcat ataaaactta tatccacaga
    63841 ataaaaaatc aaacatgtca gttttatcaa tataaagtat cgcaaggcga aaagtgatgc
    63901 aagaatactc ctaagtaaat gaaagcgcac cacatgaaat tatcaacaca tgtaatttca
    63961 tcattaggtg ctatcgaaaa taactcagga gctacttaag atgaataatg tcttactcat
    64021 gagacccgat gactcagaat ttatgatacc tgatgactgg tcagggctaa acgcaaaagg
    64081 cattacatac gaacaagata atagagtgac agagtctttt ctgcctttta aatcttttta
    64141 ttgttcaccg gtgtgcacga cagttgaact catctcataa aataattgtt ttgctatggg
    64201 tatccgctac cttctggaaa agaaaagaga cttaaggcta aatcacttta aagccttctc
    64261 tcataaatgc gagacaaaac ctgacgtgtt aattatgacc gggtgcaccg tcgctgatac
    64321 ttatagtata ttctcacgca ttgttgaaga aaatataatg aaaacgatga cacctatcgt
    64381 catcattact gggcatcact cagaagatct ttgttctctg gcatcgaaac tgggaggagt
    64441 taaaataata tcaccgacag aaaagattgc aaaaatagaa aacgctatca atgatgcttt
    64501 taacagcaag cctaaaaaaa taagattttt aacagatata ctctctcctc aacaaattga
    64561 gaccatgaaa atggttgcgc agggtagatc cgttaatgag atttcagccg caatgaatat
    64621 tagcatcaag accgtgtatg cacataaagt tcgtgctatc aataagctgg gaattaagaa
    64681 aaaagtttat gaagcgcttt tttataaagc gctaattcag ttcaactgat gtaaaaatca
    64741 ccctcgctta ataaataata tattctatgt ctttttttat aagttaaaaa aggcgggata
    64801 tcaaatcccg ccatgaaatt aactaatcat ctgcattatt ctgcgtagat ataagcctgg
    64861 gtacggatgg tgtaagagtc tggctcaaca acctgttctt tcatatggga aacgacgcga
    64921 taggcaccac ctgttccagt atttttcagc atcatataac gcttaccgcc aatctcttcg
    64981 gaggcagctg aaggaggatc ctgctcaagc gttgtcaggg aaactttcag tttatggtcc
    65041 gggttattgt caccagaaat tactgcatcc gctgaagtac tgctctccgc ataaatatca
    65101 tccgggaaag ttacagcata gtcgtggctc tgcccatcag atgcgccaac aatacctgtt
    65161 gccaaaggcg tccatgcagt taacgtaccg ctatataccc ctttcactgg ttgaatggta
    65221 ttattaacaa cgataggctt ggtcaaagta agattagctg acgcttcagt accctggctt
    65281 actaaagttc ccgcctgaga agcaaaagct gtccccgcca gcatgattgc taacaatgtt
    65341 ttcttcatta tattttctcc atgattttca tttacactgc aaaatgcagt ttctttcttt
    65401 gcatttacgt gctatcgcga aaatgcttcc accaaataat ggaaacatct tcacaaaaat
    65461 aaattatgag tttcttaatt tagaaactat aaacatgtcg ccgctatctg gtataaccct
    65521 gatgcacttt tcttcgcagg aagttggtat tttgcgatac aggattgatc tttagaataa
    65581 ccccatttca cattaaactc gccttccgca ctcaagccag aaacataagc aataccatct
    65641 tcacctacaa tgccggccat attttcagtg tctttgctat aactaatcac agcgccgaag
    65701 gggatgggtt taccctcttt ctgttttaag gttactaatc ctttatcccc gctcttcact
    65761 gccaatgtcg caggcacaac ggctccagct gtcggtacaa ccaattgact agtttgtggt
    65821 aagtcgtaat tcttacctat cgttttttca tccagaataa catcgtctct tcggtatggc
    65881 tgcactggct gtattgcata cccacgaaaa tcagtactca gtcccggcca gccattaacg
    65941 ttaacccccg ttgctccggg tgcctctata agggctaatg tcccatccac attttgacca
    66001 aatgtaagtc catcccttgt aataagtatc ccaccgctta aatttgcacc tagctgtttc
    66061 atgttttcgc tggagctgta atggctacta aactgaccat atgatccatt tttgccaact
    66121 tgcacaatgt tcatcgtgct cttcggttgt gactgatgag cgttagttat atttgtcgct
    66181 acatcccacc atacaccacc actattctga cctgataatg aaacctgata gttattaccc
    66241 tgctctcccg aacgactcat ctgtgaagac gcataaattt gattttcgtc acccagcgat
    66301 aatggcactg aaatacccac gcttaatttc cgatctactt tccatttatg cgatgtcagc
    66361 aagtcgtttt ttgcccagtt aatggagaat gttatgcctc tccataccgt actataactc
    66421 aggctggtag accgcgtgtt tcctgtgccc cgggaagaat tatacctcgt ggtattgaag
    66481 ttcaaattcc cgatattacc aaggggctgg gataaggata tcgta
//
LOCUS       DAJTUP010000008        65846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTUP010000008 DAJTUP010000000
VERSION     DAJTUP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.008"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..421)
                     /gene="folK"
                     /locus_tag="OO468_000526"
     CDS             complement(<1..421)
                     /gene="folK"
                     /locus_tag="OO468_000526"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR6257767.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPD"
     gene            complement(418..1836)
                     /gene="pcnB"
                     /locus_tag="OO468_000527"
     CDS             complement(418..1836)
                     /gene="pcnB"
                     /locus_tag="OO468_000527"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR6257768.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(1875..2801)
                     /gene="gluQRS"
                     /locus_tag="OO468_000528"
     CDS             complement(1875..2801)
                     /gene="gluQRS"
                     /locus_tag="OO468_000528"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR6257769.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(2838..3293)
                     /gene="dksA"
                     /locus_tag="OO468_000529"
     CDS             complement(2838..3293)
                     /gene="dksA"
                     /locus_tag="OO468_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR6257770.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3471..4175)
                     /gene="sfsA"
                     /locus_tag="OO468_000530"
     CDS             complement(3471..4175)
                     /gene="sfsA"
                     /locus_tag="OO468_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR6257771.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4190..4720)
                     /gene="thpR"
                     /locus_tag="OO468_000531"
     CDS             complement(4190..4720)
                     /gene="thpR"
                     /locus_tag="OO468_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR6257772.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4794..7223
                     /gene="hrpB"
                     /locus_tag="OO468_000532"
     CDS             4794..7223
                     /gene="hrpB"
                     /locus_tag="OO468_000532"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR6257773.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPKILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7419..9953
                     /gene="mrcB"
                     /locus_tag="OO468_000533"
     CDS             7419..9953
                     /gene="mrcB"
                     /locus_tag="OO468_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR6257774.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10173..12416
                     /gene="fhuA"
                     /locus_tag="OO468_000534"
     CDS             10173..12416
                     /gene="fhuA"
                     /locus_tag="OO468_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR6257775.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12467..13264
                     /gene="fhuC"
                     /locus_tag="OO468_000535"
     CDS             12467..13264
                     /gene="fhuC"
                     /locus_tag="OO468_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR6257776.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13264..14154
                     /gene="fhuD"
                     /locus_tag="OO468_000536"
     CDS             13264..14154
                     /gene="fhuD"
                     /locus_tag="OO468_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR6257777.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14151..16133
                     /gene="fhuB"
                     /locus_tag="OO468_000537"
     CDS             14151..16133
                     /gene="fhuB"
                     /locus_tag="OO468_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR6257778.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16168..17448)
                     /gene="hemL"
                     /locus_tag="OO468_000538"
     CDS             complement(16168..17448)
                     /gene="hemL"
                     /locus_tag="OO468_000538"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR6257779.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17673..19094
                     /gene="clcA"
                     /locus_tag="OO468_000539"
     CDS             17673..19094
                     /gene="clcA"
                     /locus_tag="OO468_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR6257780.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19118..19183
                     /locus_tag="OO468_000540"
     CDS             19118..19183
                     /locus_tag="OO468_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257781.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19176..19520
                     /gene="erpA"
                     /locus_tag="OO468_000541"
     CDS             19176..19520
                     /gene="erpA"
                     /locus_tag="OO468_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR6257782.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19567..20190)
                     /locus_tag="OO468_000542"
     CDS             complement(19567..20190)
                     /locus_tag="OO468_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR6257783.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20228..21028)
                     /gene="btuF"
                     /locus_tag="OO468_000543"
     CDS             complement(20228..21028)
                     /gene="btuF"
                     /locus_tag="OO468_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR6257784.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21021..21719)
                     /gene="mtnN"
                     /locus_tag="OO468_000544"
     CDS             complement(21021..21719)
                     /gene="mtnN"
                     /locus_tag="OO468_000544"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR6257785.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21803..23320
                     /gene="dgt"
                     /locus_tag="OO468_000545"
     CDS             21803..23320
                     /gene="dgt"
                     /locus_tag="OO468_000545"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR6257786.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23450..24874
                     /gene="degP"
                     /locus_tag="OO468_000546"
     CDS             23450..24874
                     /gene="degP"
                     /locus_tag="OO468_000546"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR6257787.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25029..26185
                     /gene="cdaR"
                     /locus_tag="OO468_000547"
                     /pseudo
     CDS             25029..26185
                     /gene="cdaR"
                     /locus_tag="OO468_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26274..26660)
                     /locus_tag="OO468_000548"
     CDS             complement(26274..26660)
                     /locus_tag="OO468_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR6257788.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26974..27798)
                     /gene="dapD"
                     /locus_tag="OO468_000549"
     CDS             complement(26974..27798)
                     /gene="dapD"
                     /locus_tag="OO468_000549"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR6257789.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27829..30501)
                     /gene="glnD"
                     /locus_tag="OO468_000550"
     CDS             complement(27829..30501)
                     /gene="glnD"
                     /locus_tag="OO468_000550"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR6257790.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30563..31357)
                     /gene="map"
                     /locus_tag="OO468_000551"
     CDS             complement(30563..31357)
                     /gene="map"
                     /locus_tag="OO468_000551"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR6257791.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31725..32450
                     /gene="rpsB"
                     /locus_tag="OO468_000552"
     CDS             31725..32450
                     /gene="rpsB"
                     /locus_tag="OO468_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR6257792.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32708..33559
                     /gene="tsf"
                     /locus_tag="OO468_000553"
     CDS             32708..33559
                     /gene="tsf"
                     /locus_tag="OO468_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR6257793.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33706..34431
                     /gene="pyrH"
                     /locus_tag="OO468_000554"
     CDS             33706..34431
                     /gene="pyrH"
                     /locus_tag="OO468_000554"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR6257794.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34723..35280
                     /gene="frr"
                     /locus_tag="OO468_000555"
     CDS             34723..35280
                     /gene="frr"
                     /locus_tag="OO468_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR6257795.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35372..36568
                     /gene="ispC"
                     /locus_tag="OO468_000556"
     CDS             35372..36568
                     /gene="ispC"
                     /locus_tag="OO468_000556"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR6257796.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36757..37515
                     /gene="ispU"
                     /locus_tag="OO468_000557"
                     /gene_synonym="uppS"
     CDS             36757..37515
                     /gene="ispU"
                     /locus_tag="OO468_000557"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR6257797.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37528..38385
                     /gene="cdsA"
                     /locus_tag="OO468_000558"
     CDS             37528..38385
                     /gene="cdsA"
                     /locus_tag="OO468_000558"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6257798.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38397..39749
                     /gene="rseP"
                     /locus_tag="OO468_000559"
     CDS             38397..39749
                     /gene="rseP"
                     /locus_tag="OO468_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR6257799.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39779..42211
                     /gene="bamA"
                     /locus_tag="OO468_000560"
     CDS             39779..42211
                     /gene="bamA"
                     /locus_tag="OO468_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR6257800.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42333..42818
                     /gene="skp"
                     /locus_tag="OO468_000561"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42333..42818
                     /gene="skp"
                     /locus_tag="OO468_000561"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR6257801.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42822..43847
                     /gene="lpxD"
                     /locus_tag="OO468_000562"
     CDS             42822..43847
                     /gene="lpxD"
                     /locus_tag="OO468_000562"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR6257802.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43952..44407
                     /gene="fabZ"
                     /locus_tag="OO468_000563"
     CDS             43952..44407
                     /gene="fabZ"
                     /locus_tag="OO468_000563"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR6257803.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44411..45199
                     /gene="lpxA"
                     /locus_tag="OO468_000564"
     CDS             44411..45199
                     /gene="lpxA"
                     /locus_tag="OO468_000564"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR6257804.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45199..46347
                     /gene="lpxB"
                     /locus_tag="OO468_000565"
     CDS             45199..46347
                     /gene="lpxB"
                     /locus_tag="OO468_000565"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR6257805.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46344..46940
                     /gene="rnhB"
                     /locus_tag="OO468_000566"
     CDS             46344..46940
                     /gene="rnhB"
                     /locus_tag="OO468_000566"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR6257806.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            46977..50459
                     /gene="dnaE"
                     /locus_tag="OO468_000567"
     CDS             46977..50459
                     /gene="dnaE"
                     /locus_tag="OO468_000567"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR6257807.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50472..51431
                     /gene="accA"
                     /locus_tag="OO468_000568"
     CDS             50472..51431
                     /gene="accA"
                     /locus_tag="OO468_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR6257808.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51530..53671
                     /gene="ldcC"
                     /locus_tag="OO468_000569"
     CDS             51530..53671
                     /gene="ldcC"
                     /locus_tag="OO468_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR6257809.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53728..54117
                     /locus_tag="OO468_000570"
     CDS             53728..54117
                     /locus_tag="OO468_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6257810.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54182..55480
                     /gene="tilS"
                     /locus_tag="OO468_000571"
     CDS             54182..55480
                     /gene="tilS"
                     /locus_tag="OO468_000571"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR6257811.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55529..55789)
                     /gene="rof"
                     /locus_tag="OO468_000572"
     CDS             complement(55529..55789)
                     /gene="rof"
                     /locus_tag="OO468_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR6257812.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55776..55976)
                     /locus_tag="OO468_000573"
     CDS             complement(55776..55976)
                     /locus_tag="OO468_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR6257813.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56142..56687
                     /locus_tag="OO468_000574"
     CDS             56142..56687
                     /locus_tag="OO468_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR6257814.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56684..57106
                     /gene="arfB"
                     /locus_tag="OO468_000575"
     CDS             56684..57106
                     /gene="arfB"
                     /locus_tag="OO468_000575"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6257815.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57120..57830
                     /gene="nlpE"
                     /locus_tag="OO468_000576"
                     /gene_synonym="cutF"
     CDS             57120..57830
                     /gene="nlpE"
                     /locus_tag="OO468_000576"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR6257816.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(57985..58809)
                     /locus_tag="OO468_000577"
     CDS             complement(57985..58809)
                     /locus_tag="OO468_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR6257817.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(58862..60580)
                     /gene="proS"
                     /locus_tag="OO468_000578"
     CDS             complement(58862..60580)
                     /gene="proS"
                     /locus_tag="OO468_000578"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR6257818.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60691..61398)
                     /gene="tsaA"
                     /locus_tag="OO468_000579"
     CDS             complement(60691..61398)
                     /gene="tsaA"
                     /locus_tag="OO468_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR6257819.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61395..61799)
                     /gene="rcsF"
                     /locus_tag="OO468_000580"
     CDS             complement(61395..61799)
                     /gene="rcsF"
                     /locus_tag="OO468_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR6257820.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61917..62732)
                     /gene="metQ"
                     /locus_tag="OO468_000581"
     CDS             complement(61917..62732)
                     /gene="metQ"
                     /locus_tag="OO468_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR6257821.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62772..63425)
                     /gene="metI"
                     /locus_tag="OO468_000582"
     CDS             complement(62772..63425)
                     /gene="metI"
                     /locus_tag="OO468_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR6257822.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63418..64449)
                     /gene="metN"
                     /locus_tag="OO468_000583"
     CDS             complement(63418..64449)
                     /gene="metN"
                     /locus_tag="OO468_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR6257823.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64637..65212
                     /gene="gmhB"
                     /locus_tag="OO468_000584"
     CDS             64637..65212
                     /gene="gmhB"
                     /locus_tag="OO468_000584"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR6257824.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65576..>65846
                     /locus_tag="OO468_000585"
     rRNA            65576..>65846
                     /locus_tag="OO468_000585"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15564 a        16325 c        18167 g        15790 t
ORIGIN      
        1 catcaggaaa cgccagctcc ggtgcaatct caaacagcgg ccacagcata aatccacgat
       61 tcttcatatc gtagtgcgga acggtcaggc gttcggtatt tatcacttca ttaccaaaca
      121 gcatgatatc gagatccagc gtgcgtggtc cccagcgttc agctttacga acacgaccct
      181 gttgcagttc tatgcgctgt gtatggttga gtaactcttc cggggcgggc gaagtttcaa
      241 gcgccacggc ggcgttgagg taatcgggtt gatcttgcgg ccccagcggt ggggtgcggt
      301 aaaacgaaga aacggcaaga atgcggcttt cagggatatc gcctaatgct ttcagggcag
      361 cattgacctg ctccagcgga gaggccagat tgctgcctat ggcaatatac gccactgtca
      421 tgtggtaccc tcacgacgcg gtgcgcgttt gcgtggacga cgagtacgac gacgcggtga
      481 cggttcttca tccagctcgt taagcatccc tttttgatct ggtggtgcgg aaacctggaa
      541 ctcaccccac catttcacca gacgctgcag ttcagcatta cgttcaactt cagctcgcaa
      601 ggccaacagg tcataagccg cacggaactt aggatgctcc agcagtttcc atgcgcgttt
      661 accctgacga cgggacatac gcaactgcaa ctgccagata tcgcgggtta acgtcgtcag
      721 acgtttcggg attgccagtg aacggcaggc ttcgtccagc acgtcgttca tcgccagcgc
      781 gaaagcgtcg tgataggtca ggccgctttc ctgggcgatc ttctgtgccg tctccagcag
      841 tgggtaccag aacatggcgg caaacaagaa cgccgggttc acgcgcatat cgttatggat
      901 acgcgtatcg gtattcttca gcacctgttc aatgatccgc tccatcgggc tgtcgccatt
      961 ttccgtgaag tagcgggtaa tggtcgggaa cagcggctgg aacagatgat attcacacaa
     1021 cagcttatag gtttcgtaac cgtagcccgc ttgtagcagt ttaagcgatt cttcaaacag
     1081 gcgtgccggt gggatatcgt tcagcagggt agcgaggcga gggatcggtt ctgcggtttc
     1141 cgggctgatg cgcataccca atttggcggc aaaacgtacc gcgcgcagca tacgtaccgg
     1201 atcttcacgg tagcgcgttt ccgggttacc aatcagacgg ataacgccgt ccttcagatc
     1261 cttcatacca ccaacgtaat cacggacggt aaaatccgct acgctgtaat acaggctgtt
     1321 gatagtgaaa tcgcggcgct gggcgtcttc ttcgatggag ccgaaaatgt tgtcgcgcag
     1381 caacatgccg ttttgcccgc gttgggaggt cgtgcggtcg ctgacgttac cttcgtggtg
     1441 tccacggaag gtcgcaactt cgataatctc cgggccaaac attacatgag ccagacggaa
     1501 acggcgaccc accaggcggc agttacggaa cagtttgcgc acctgctcag gcgtggcgtt
     1561 agtggttacg tcaaaatctt tcggcttttt gccaagtaac aggtcgcgca cgccgccgcc
     1621 aaccagccag gcttcgtatc ccgctttgtt gagcctgtac attaccttca gggcattttc
     1681 actgatatct ttgcgggaaa tagcatgctg ctcacgcggg atcaccgtca cctgtggacg
     1741 ggcgactgcc tgttcagcct cgctttcctc gcggcttagc accttgcggc aaaaattagc
     1801 gactcgggta aaaatagtac acctcggtag tgtcaaacat cattcaggac aaaaaaatag
     1861 cggctaatca tagctcagca tgacgcattt gagaatgttg aatttacaat tgccgactcg
     1921 ggcacggcgg tcagcatcca gtttttgacg gctgactgaa ggatttgctc gacgctgaaa
     1981 tcctgccagt gtgcttctgc ctgctgcccc agaaattgaa gtgccgcgat tagtaccggg
     2041 cgtggatcgc ctttcggcaa cgcaggcgca tgattctgct tggaaagttt agcgccttgt
     2101 ggattaagcg ccagcggcag atgaatgtaa tctggcactt tccagccaaa aagctggtac
     2161 agcgggattt gccttactgt tggttcaatc agatcagccc cacgcactat ttctgtaacg
     2221 ccctggaaat gatcatcaac cacaacagcc aggttgtagg cgaacaaccc atcacggcga
     2281 tgaatgataa aatcttcccg tgccagtttt tcgtcggcgt gaataatgcc tcgcagcagg
     2341 tcagtaaatt gcgtgaccgg atgctgctgg cggatacgca ctgcggcgtt gtctggtcca
     2401 tgatgcaaca cccggcaatg accgtcgtaa ataccgccaa tgctttgaat acgcgcacgc
     2461 gtacaggtgc agtaataact tagtccttgt tcatgtaacc aggcgagtgc ttcacgatag
     2521 gcgtcgtgac gttgcgattg ccagagaaca tcgccgtccc agtgcagacc gtaatgttcc
     2581 agctggcgca ggatagtttc tgcggcaccg ggaacttcac gaggcgggtc gatatcttct
     2641 atgcgtacca gccagcgacc ttgccgggcg cgagcctgca aatagctgcc gagcgcggcg
     2701 atcagagagc caaaatgaag ctcgccggaa ggagaggggg cgaagcggcc aatatactgt
     2761 gtgtctgtca tctctttgaa caaaaaataa ggcgggagca tttcccgcct gtggtaaacg
     2821 tgatggaacg gctgtaatta gccagccatc tgtttttcgc gaatttcagc cagcgttttg
     2881 cagtcgatgc acagatcggc tgtcgggcgc gcttccagac ggcgaatacc aatttcaaca
     2941 ccgcaggatt cgcagtagcc gaaatcttcg tcttccactt ttttcagcgt cttctcgatc
     3001 tttttgatca gcttacgctc gcgatcgcgg ttacgcagtt cgaggctgaa ctcttcttcc
     3061 tgggctgcac ggtctaccgg gtccgggaag ttggctgctt catcctgcat atgtgtaacg
     3121 gtgcgatcga cttcatccct gagttgatta cgccatgctt ccagaatacg acggaagtgc
     3181 gccagctggg cttcattcat atactcttcg cccggcttct cctgatatgg ttccacccca
     3241 gcgatggcga gaatactcag ggacgatgtt ttacggtttt gcccttcttg catgttgctt
     3301 ctccttaaca cgcactatcg atccccatgt tcgggggaaa aatgaggccg ctataaatag
     3361 cagatgcttt tccggatagc aattatctaa acgtaacact tgacaactgt gtgaggaaaa
     3421 gcgtatttgc gcacgcgacc agaatgtaaa ttaaccagtt acttacttta ctacaatgta
     3481 accggcagtg attttttaag agccatgcct tcagcagaaa tttccgcttt gtaagccaga
     3541 atttctaccc ccctctgttg agcttctgac aatagttgcg cgtatttctc atcgatgtgg
     3601 cgcgcgggtg aaaaccgtgt aatggctgaa tgcagcacgg cgaaaaaaat aaccgcacgc
     3661 tggccttcag ccgctacgct catcaactcc cgaaggtgtt tctgacctcg ttcagtgacc
     3721 gcatcgggaa aatatccctg ttcgttctcc gctaacgtaa ccgatttcac ttcaatatag
     3781 cagtctggac gcgaatccgc ctgcaacata aagtcaatac ggctgcgttc ggagccgtat
     3841 tttacttcgc ttttcagcga gctatagcct gacagttctg aaattgattc attaaggata
     3901 gcctctttcg tcaacctgtt agcccaaagc gtattgacgc aaataaatgc gccgctctgg
     3961 ctttgagtta attcccaggt gtgtgggtat ttccgtttgg tgttgtctga agtcgaatac
     4021 cagacggtat cgccaggcgt tgcacaaccg gtcatcgcac ccgtattcgg gcagtgtagc
     4081 gttaattcgc gaccatcggg tgtgatcaca tcggctaaaa aacgtttgta acgctgaatt
     4141 agcgtcgcgc gctgtagagg gggagaaaat tccattgcga caatccttgt tattgcgtta
     4201 gcgcccagcg ttttagcggc gtgtagcgtg tgcgtccacg ggcaaacgag gaggcgtaaa
     4261 gggtgaactc cgtcaccgca taagaccagt taaaacccgg cggcgggaat gtcaccgcct
     4321 cgctggcgtc gcgcaataag gtaatatgtg gatgaaacgg acgattgctt tgaaaacaac
     4381 cgctgcgggc agcctgtgaa cggagcatat tcgccagctg gattaagccg cgtggaggct
     4441 gacgcatccc taaccacacc acacgcgaac gcagccattg tccggcgtca tcaagcgtga
     4501 gtgtgaaacc aggttgacga atccgtccgg ctaaaagaga aagcgccttc tctttctctg
     4561 cgctcacttc gcctaaaaat gccagagtca ggtgcaaatt atcggcggcg accggacgtc
     4621 ccgcctcagg tgggaagtgt gtggcgcgcc aatggataat ctgttcgcgg atttctgcag
     4681 gtaagtcgat agcaaagaac agacgttgcg gttcagacat gtgaggcact cggttatgaa
     4741 ttaccgcgat gctacaatgt ggcgcgaaga atgttaaccc tctggagcgt tttgtgtcgt
     4801 cgttgcccgt tgctgccgtc ttacctgaat tacttaccgc tctcgattgt gcgccgcagg
     4861 tattattaag tgcgccgacc ggggccggga aatcaacctg gctgccgctg caactgctgg
     4921 cgcatcccgg cattaacggg aaaattatcc tgctggagcc gcgtcgtctg gcggcgcgta
     4981 acgtcgcgca acggctggcg gagctgctta acgaaaagcc aggcgatacc gttggctacc
     5041 ggatgcgtgc gcaaaactgc gtcgggctga atacccgcct ggaagtggtt accgaaggcg
     5101 tgctgacgcg gatgatccag cgtgacccgg aactgagcgg tgttggattg gtgatccttg
     5161 atgagtttca tgagcgcagt ttgcaggcgg atttggcgtt ggcgctgtta ctcgatgtgc
     5221 aacaaggtct gcgtgatgac cttaaactgc tgattatgtc ggctacgctg gacaatgacc
     5281 gcttgcagca aatgctgccg gaagcgcccg tcgtcatctc agaagggcgc tcgtttccgg
     5341 ttgaacgccg ttatttaccg ctgcccgcgc atcagcgttt tgacgaagcc gttgcggtag
     5401 ccaccgccga aatggtgcgt caggaaagtg gatcattact gttattttta cctggcgtcg
     5461 gagaaattca gcgcgtgcag gaacaactgg cttcgcgcat cggcagtgat gtggtgctct
     5521 gcccgctgta tggcgtgttg tcactgaacg atcagcgaaa agcgatcctc ccggcaccgc
     5581 aagggatgcg caaagtggtg ctggcgacca atattgctga aaccagttta accatcgaag
     5641 gtattcgtct ggtggtggat tgtgcccagg agcgtgtggc gcgctttgat ccgcgcacgg
     5701 ggcttacgcg gttgattact caacgcgtta gtcaggcatc catgacgcag cgtgccgggc
     5761 gcgccgggcg tctggagccg ggtatcagcc tgcatttaat cgccaaagaa caagcagaac
     5821 gcgccgcggc gcaaagtgag ccgaagatct tacaaagcga tctttccggt ttgctgatgg
     5881 aattactgca atggggatgc agcgatccgg cgcagatgag ctggctggat caaccgccaa
     5941 cggtgaatct actggccgcg aaacgcctgt tacaaatgct gggggcgctg gagggtgaac
     6001 ggcttagtgc gcaagggcaa aaaatggcag cgctgggtaa cgatccgcgt ttagcggcaa
     6061 tgctggttaa cgcaaagagc gatgacgaag ctgctaccgc ggcaaaaatt gccgccattc
     6121 tcgaagagcc gccacggatg ggcaatagtg acctgggcgt ggcgttttcg cgcaatcaac
     6181 cagcctggca gcaacgtagt cagcaactgt taaaacgctt aaacgtacgc ggcggtgagg
     6241 cagacagttc gcttatcgcg ccgctacttg ccggggcgtt tgccgatcgc attgctcatc
     6301 gccgtgggca agatggacgc tatcaactgg caaacagcat gggagcgatg ctcgatgccg
     6361 acgacgcgct aagccgccac gaatggttga tcgcaccgtt attattgcag ggcagcgcct
     6421 cgccggatgc gcggatttta ctggcgctac cggtcgatat tgatgaatta gtacaacgct
     6481 gtccgcagct ggtgcagcaa tctgacaccg tggaatggga tgacgcgcaa ggtacgctga
     6541 aagcctggcg tcggctacaa atcggtcagt tgacggtgaa agtgcagccg ctggcgaaac
     6601 cgtcagaaga cgagttgcat caggcgatgc ttaatggcat ccgtgataaa ggtttaagcg
     6661 tgctcaactg gacggcggaa gcggaacagc tacgcttgcg tttgttatgc gccgcaaagt
     6721 ggttgccgga atatgactgg ccagcggttg atgatgaaag tttgttggcg acgctggaaa
     6781 cgtggctgct gccacatatg accggcgtac attcactacg cggcctgaaa tcactcgaca
     6841 tttatcaggc actacgcgga ttacttgatt ggggaatgca gcaacgtctg gatagtgaat
     6901 tgcctgcgca ttacactgtg ccgacgggaa gccggatcgc cattcgttat catgaagata
     6961 acccgcccgc gctggcggtg agaatgcagg agatgtttgg tgaggccact aatccgacga
     7021 tcgcccaggg gcgcgtgccg ctggtgctga agttgctttc ccctgcccaa aggccgctgc
     7081 aaatcacgcg tgatttgggg gccttctgga aaggggcgta tcgtgaggtg caaaaggaga
     7141 tgaaagggcg ttatcccaaa cacgtctggc cggacgaccc ggcaaacacg gcaccgacgc
     7201 gacggacgaa aaagtattcg taaggttggt tttctccctc tccctgtggg agagggccgg
     7261 ggtgagggca tcagcgcgca cgttcaccct caccctaacc ctctccctca agggagaggg
     7321 gaccgatcga gcgcaaattt tgagagatat cttcttctgt cttgtaacag aagaacagaa
     7381 aatcgggctt ttgcgcctga atattgcgga gaaaaagcat ggccgggaat gaccgcgagc
     7441 caattggacg caaagggaaa ccgacgcgtc cggtcaaaca aaaggtaagc cgtcgtcgtt
     7501 acgaagatga cgatgattac gacgattatg atgactatga ggatgaagaa ccgatgccgc
     7561 gcaaaggtaa gggcaaaggc aaagggcgta agcctcgtgg caaacgcggc tggctatggc
     7621 tactgctaaa actggctatc gtttttgccg tgctgatcgc catttacggc gtttatctcg
     7681 atcaaaaaat tcgtagccgt attgatggca aggtctggca actgcccgcg gcagtttatg
     7741 gccgaatggt caaccttgaa ccagatatga ccatcagcaa gaacgaaatg gtgaagctgc
     7801 tggaggcaac acagtatcgt caggtgtcga aaatgacccg tcctggcgaa tttaccgtgc
     7861 aggccaacag cattgagatg atccgccgtc cgtttgattt cccggacagt aaagaagggc
     7921 aggtgcgtgc gcgtctgacc tttgatggtg atcatctggc gaccatcgtc aatatggaga
     7981 acaaccgtca gttcggtttc ttccgtcttg atccgcgtct gatcaccatg atctcctcgc
     8041 caaacggtga gcagcgtttg tttgtgccgc gcagtggttt ccctgatttg ctggtggata
     8101 ctttgctggc gacagaagac cgtcattttt acgagcatga tggaatcagt ctctactcca
     8161 ttgggcgtgc ggtgctggca aacctgaccg caggtcgcac ggtgcagggt gcgagtacgc
     8221 tgacgcaaca gctggtgaaa aacctgttcc tctccagcga gcgttcttac tggcgtaaag
     8281 cgaacgaagc ttacatggcg ctgatcatgg acgcgcgtta cagcaaagac cgtattcttg
     8341 agctgtatat gaacgaggtg tatctcggtc agagcggcga caacgaaatc cgcggcttcc
     8401 cgctggcaag cttgtattac tttggtcgcc cggtagaaga gctaagcctc gaccagcagg
     8461 cgctgttagt cggtatggtg aaaggggcgt ccatctacaa cccgtggcgt aacccgaaac
     8521 tggcgctgga gcgacgtaat ctggtgctgc gtctgctgca acagcaacag attattgatc
     8581 aagaactcta tgacatgttg agtgcccgtc cgctgggggt tcagccgcgt ggtggggtga
     8641 tctctcctca gccagccttt atgcagctgg tgcgtcagga gttgcaggca aaactgggcg
     8701 ataaggtaaa agatctctcc ggcgtgaaga tcttcactac ctttgactcg gtggcccagg
     8761 acgcggcaga aaaagccgcc gtggaaggca ttccggcact gaagaaacag cgtaagttga
     8821 gcgatcttga aactgcgatt gtggtcgtcg accgctttag tggtgaagtt cgtgcgatgg
     8881 tcggaggttc tgagccgcag tttgcgggct acaaccgtgc gatgcaggcg cgtcgttcga
     8941 ttggttccct tgcaaaacca gcgacttatc tgacggcttt aagccagccg aaaatctatc
     9001 gcctgaacac gtggattgcc gatgcgccaa tcgcgctgcg tcagccgaat ggccaggtct
     9061 ggtcaccgca gaacgatgac cgtcgttata gcgaaagcgg cagagtgatg ctggtggatg
     9121 cgttgacacg ttcgatgaac gtgccgacgg taaatctggg gatggcgctg gggctgcctg
     9181 cggttacgga gacctggatc aaactgggcg taccgaaaga tcagttgcat ccggttccgg
     9241 caatgctgct gggggcgttg aacttaacgc caatcgaagt ggcgcaggca ttccagacca
     9301 tcgccagcgg tggtaaccgt gcaccgcttt ctgcactgcg ttcggtaatc gcggaagatg
     9361 gtaaggtgct gtatcagagc ttcccgcagg cagaacgcgc tgttccggcg caggcggcgt
     9421 atctgacact atggaccatg cagcaggtgg tacaacgtgg tacgggtcgt cagcttgggg
     9481 cgaaataccc gaacctgcat ctggcaggga aaacagggac taccaacaat aacgtagata
     9541 cctggtttgc aggcattgac ggcagcacgg tgaccatcac ctgggtaggc cgtgataaca
     9601 accagccgac caaactgtat ggagccagcg gggcaatgtc gatttatcag cgttatctgg
     9661 ctaaccagac gccaacgccg ctgaatcttg ttccgccaga agatattgca gatatgggcg
     9721 tggactacga cggcaacttt gtttgcagcg gtggcatgcg tgtcttgccg gtctggacca
     9781 gcgatccgca atcgctgtgc cagcagagcg agatgcagca gcagccgtca ggcaatccgt
     9841 ttgatcagtc ttctcagccg cagcaacagc cgcaacagca acctgctcag caagagcaga
     9901 aagacagcga cggtgtagcc ggttggatca aggatatgtt tggtagtaat taacatctaa
     9961 gcgtgaaata ccggatggcg agttgccatc cggtaaaata acatcccatc taagatatta
    10021 accctttctt ttcatctggt tgtttattaa cccttcagga acgctcagat tgcgtaccgc
    10081 ttgcgaaccc gccagcgttt cgaatattat cttatcttta taataatcat tctcgtttac
    10141 gttattattc actttacatc agagatatac caatggcgcg ttccaaaact gctcagccaa
    10201 aacactcact gcgtaaaatc gcagttgtag tagccacagc ggttagcggc atgtctgttt
    10261 atgcacaggc agcggttgaa ccgaaagaag acactatcac cgttaccgct gcacctgcgc
    10321 cgcaagaaag cgcatggggg cctgctgcaa ctattgcggc gcggcagtca gctaccggca
    10381 ctaaaaccga tacgccgatt caaaaagtgc cacagtctat ttctgttgtg accgccgaag
    10441 agatggcgct gcatcagccg aagtcggtaa aagaagcgct tagctacacg ccgggtgtct
    10501 ctgttggtac gcgtggcgca tccaacacct atgaccacct gatcattcgc ggttttgcgg
    10561 cagaaggcca aagccaaaat aactatctga atggcctgaa gttgcagggc aacttctata
    10621 acgatgcggt cattgacccg tatatgctgg aacgcgctga aattatgcgt ggcccggttt
    10681 ccgtgcttta cggtaaaagc agtcctggcg gtctgttgaa tatggtcagc aagcgtccga
    10741 ccaccgaacc gctgaaagaa gttcagttta aagccggtac tgacagcctg ttccagactg
    10801 gttttgactt tagcgatgcg ttggatgatg acggtgttta ctcttatcgc ctgaccggtc
    10861 ttgcgcgttc tgccaatgcc cagcagaaag ggtcagaaga gcagcgttat gctattgcac
    10921 cggcgttcac ctggcgtccg gatgataaaa ccaatttcac cttcctttct tacttccaga
    10981 acgagccgga aaccggttat tacggctggt tgccgaaaga gggaaccgtt gagccgctgc
    11041 cgaacggtaa gcgtctgccg acagacttta atgaaggggc gaagaacaac acctattctc
    11101 gtaatgagaa gatggtcggc tacagcttcg atcacgaatt taacgacacc tttactgtgc
    11161 gtcagaacct gcgctttgct gaaaacaaaa cctcgcaaaa cagcgtttat ggttacggcg
    11221 tctgctccga tccggcgaat gcttacagca aacagtgtgc ggcattagcg ccagcggata
    11281 aaggccatta tctggcacgt aaatacgtcg ttgatgatga gaagctgcaa aacttctccg
    11341 ttgataccca gttgcagagc aagtttgcca ctggcgatat cgaccacacc ctgctgaccg
    11401 gtgtcgactt tatgcgtatg cgtaatgaca tcaacgcctg gtttggttac gacgactctg
    11461 tgccactgtt caatctgtac aatccggtga atcccgattt cgacttcaat gccaaagatc
    11521 cggcaaactc cggcccttac cgcattctga ataaacagaa acaaacgggc gtttatgttc
    11581 aggatcaggc gcagtgggat aaagtgctgg tcaccctagg cggtcgttat gactgggcag
    11641 atcaagaatc tcttaaccgc gttgctggga cgaccgataa acgtgatgac aaacagttta
    11701 cctggcgtgg tggtgttaac tacctgtttg ataatggtgt aacaccttac ttcagctata
    11761 gcgaatcgtt tgaaccttct tcgcaagttg ggaaggatgg taatattttc gcaccgtcta
    11821 aaggtaagca gtatgaagtc ggcgtgaaat atgtaccgga agatcgtccg attgtagtta
    11881 ctggtgccgt gtataatctc actaaaacca acaacctgat ggcggaccct gagggttcct
    11941 tcttctcggt tgaaggtggc gagatccgcg cacgtggcgt agaaatcgaa gcgaaagcgg
    12001 cgctgtcggc gagtgttaac gtagtcggtt cttatactta caccgatgcg gaatacacca
    12061 ccgatactac ctataaaggc aatacgcctg cacaggtgcc aaaacacatg gcttcgctgt
    12121 gggctgacta taccttcttt gacggtccgc tttcaggtct gacgctgggc accggtggtc
    12181 gttatactgg ctccagctat ggtgatccgg ctaactcctt taaagtggga agttatacgg
    12241 tcgtggatgc gttagtgcgt tatgatctgg cgcgagtcgg catggcgggc tccaacgtgg
    12301 cgctgcatgt taacaacctg ttcgatcgtg aatacgtcgc cagctgcttt aacacttatg
    12361 gctgcttctg gggcgcagaa cgtcaggtcg ttgcaaccgc aacctttcgt ttctaatttc
    12421 tcttttgggg cacggatttc cgtgcccatt tcacaagttg gctgttatgc aggaatacac
    12481 gaatcattcc gataccactt ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac
    12541 gcttttgcat ccgctgtcgt taacctttcc tgccgggaaa gtgaccggtc tgattggtca
    12601 caacggttct ggtaaatcca ctctgctcaa aatgcttggt cgtcatcagc ctccgtcaga
    12661 aggggagatt cttcttgatg cccagccgct ggaaagctgg agcagcaaag cgtttgcccg
    12721 caaagtggct tatttgccgc agcagcttcc tccggcagaa gggatgaccg tgcgtgaact
    12781 ggtggcgatt ggtcgttacc cgtggcatgg cgcgctgggg cgctttggtg cggcagatcg
    12841 cgaaaaggtc gaggaagcta tctcgctggt tggcttaaaa ccgctggcgc atcggctggt
    12901 cgatagtctc tctggcggcg aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga
    12961 tagccgttgt ctgttgctcg acgaaccgac ttcagcgctg gatatcgccc accaggttga
    13021 tgtgctggcg ctggtgcacc gtttaagtca ggaacgtggc ctgacggtca ttgccgtgtt
    13081 gcacgatatc aatatggcgg cacgctactg tgattatctg gtcgccctgc gcagtggtga
    13141 aatgattgct cagggaacgc ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta
    13201 tggcatcccg atgggtattt tgccgcatcc ggcgggtgct gcacctgtga gttttgttta
    13261 ttgatgagcg gcttacctct tatttcgcgc cgtcgactgt taacggcgat ggcgctttct
    13321 ccgttgttat ggcagatgaa taccgcccac gcggcggcta ttgaccccaa tcgtattgtg
    13381 gcgctggagt ggttgccggt ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg
    13441 gcggatacca tcaactatcg cctgtgggtc agcgaaccac cattgccgga ctcagtgatt
    13501 gacgtcggtt tgcgtacaga acctaacctt gaactgctga ccgaaatgaa accgtcgttt
    13561 atggtctggt cggcaggata tggcccttca tcagaaatgc tggcacgtat tgcgccgggt
    13621 cgcggattta acttcagtga cggcaaacat ccgttggcga tggcgcgtaa atcgctgacg
    13681 gaaatggcag atttacttaa cctgcaaagc gcagcggaaa cgcatttagc gcaatacgaa
    13741 gactttatcc gcagcatgaa accccgcttt gtgaagcgtg gtgcacgccc gttactgctg
    13801 acgacgctta tcgatccgcg ccatatgctg gtcttcggtc caaacagctt gttccaggaa
    13861 attctcgatg agtacggcat cccaaatgcc tggcaagggg aaaccaactt ctggggcagt
    13921 accgccgtca gtatcgatcg tctggcagcg tataaagacg ttgatgtgct ctgttttgat
    13981 cacgacaaca gcaaagacat ggatgcgcta atggcaaccc cgctgtggca ggccatgccg
    14041 tttgtccgta cagggcgctt tcagcgcgta cccgcagtct ggttttatgg tgcaacgctt
    14101 tcggcaatgc actttgtgcg tgttctggat aacgccatcg gaggtaaagc gtgagtaaac
    14161 gaattgcgct tttcccggcg ttattgctgg cgctgttagt gattgtcgct acggcgctca
    14221 cctggatgaa cttctcacag gcgctgccgc gcagtcagtg ggcgcaggcc gcctggtcgc
    14281 cggatattga cgtcatcgag cagatgattt ttcactacag cttgttgccg cgtctggcga
    14341 tttcgctgct ggtgggcgcg ggcctggggc tggtgggcgt gctgtttcag caagtactgc
    14401 gtaacccgct ggcggaaccg acgacgttgg gcgttgcaac aggcgcgcaa ctggggatta
    14461 ccgtcaccac gctctgggcg atccctagcg cgatggcgag ccagtttgct gcgctggcag
    14521 gggcttgcgt tgttggctta attgtctttg gcgtcgcgtg ggggaaacgg ctgtcgccgg
    14581 taacgctgat tttcgcgggg ttggtagtga gcctttattg cggcgcaatc aatcagttac
    14641 tggttatctt ccatcatgac cagttgcaaa gcatgtttct gtggagcact ggaacgctga
    14701 cgcaaaccga ctggggcggt gttgagcgtt tatggccgca gttgctgggc ggtgtgatgc
    14761 tgacgttgct gctacttcgt ccgttaaccc tgatggggct tgatgatggc gtggcgcgca
    14821 atctcgggct ggccttgtcg cttgcgcgtc tggcggcgct gtcgctggcg attgtcatca
    14881 gtgcgctgct ggtgaacgct gtggggatta tcggctttat cgggttgttc gcgccgctgc
    14941 tggcaaaaat gctgggggcg cggcgtctgc tgccacgact gatgctggcg tcgttgattg
    15001 gtgcgctgat cctctggctt tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg
    15061 aagtgtccac cggttcggtc actgcgttga tcggtgcgcc gctgctactg tggctgctgc
    15121 cgcgtttacg cagcattagc gcgccggata tgaaggttaa cgatcgtgtc gcgactgaac
    15181 gccaacatgt gctggcgttt gccctcgcgg gcggcgtgct gttgttgatg gctgtggtgg
    15241 tggcgctgtc gtttggtcgt gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg
    15301 aggatttaat gccctggcgc tggccgcgaa ttatggcggc gctgtttgcg ggcgtcatgc
    15361 tggcggtagc gggctgtatt attcagcgac tgaccggcaa cccgatggca agcccggaag
    15421 tgctggggat tagctccggc gcggcgtttg gtgtggtgtt aatgctgttt ctggtaccgg
    15481 gtaatgcctt tggctggttg ttacccgcag gcagtctcgg ggcggcggtg acgctgttga
    15541 tcattatgat cgccgccgga cgcggtggat tttccccaca ccgcatgtta ctggcgggga
    15601 tggcgttaag taccgcgttc accatgcttt tgatgatgtt gcaggcaagt ggtgacccgc
    15661 gaatggcgca agtgctgacc tggatttccg gttcgaccta caacgcgacc gatgctcagg
    15721 tctggcgcac cggaattgtg atggtgattt tgctggcgat tactccgctg tgccgccgct
    15781 ggctgaccat tttaccgctg ggtggtgata ccgcgcgggc ggtagggatg gcgctgacgc
    15841 cgacgcgaat tgcgctgctg ctgttagcgg cttgcctgac ggcgaccgcg acgatgacta
    15901 ttggaccgtt gagttttgtt ggtttaatgg caccgcatat tgcgcggatg atgggctttc
    15961 gacggacgat gccacacatc gtaatttcgg cgctggtggg tggtttactg ctggtgttcg
    16021 ctgactggtg tgggcggatg gtgctgtttc cattccagat cccggcgggg ctgctgtcga
    16081 cctttatcgg tgcgccatat tttatctatt tgctgagaaa gcagagccgt taatagaatg
    16141 cctcgcccgg tgggcgaggc aaaattatca caacttcgcg aacacccgac gtgcagcatc
    16201 gatggtgtta ttgatatctt ccatgctgtg cgccacagac ataaagcctg cttcaaacgc
    16261 tgacggtgcc aggtaaacac cttcatccag catcatatgg aagaaacgct taaaacgctc
    16321 gacgtcacag gccatcacat cctgatagca cgtcacggac tcggcgtcgg taaagaagat
    16381 accgaacata ccaccgacgt ggttaacgac cagcggaatt cccgcttctt ctgccgcttc
    16441 cagcagacct tctgccagac gtgttgtcag ctcatccagc gtttcgtgaa tgcccggctg
    16501 cgcgacttca ttcagacagg cgaaacccgc tgccatcgca atcgggttac cggaaagcgt
    16561 acccgcctga tagaccggac ctgtcggggc cagcgcatcc attacatcac gacgaccacc
    16621 gaatgcgcct accggcattc caccgccgat gatttttccg aggcaggtta aatctggcac
    16681 tacggcgaaa taatcctgtg cgccagctag cgctacgcgg aaaccggtca tcacttcatc
    16741 gatgatcagc aacgcgccaa attcgtcgca cagcgcacgc agacctggca ggaactctgg
    16801 cagcggcgga acacagttca tattgcctgc caccggctcg acgataatac aggcaatctc
    16861 ttgcgggtat tgctcaaacg cggcgcgtac agaggccaga tcgttataag tacaggttaa
    16921 ggtatgtttg gcgaaatctg ccggaacgcc cggcgagttt ggctggccta acgtgagtgc
    16981 gccagaaccg gctttcacca gcaggcagtc agcgtgaccg tggtaacaac cttcaaattt
    17041 aatgattttg tcgcgaccgg taaaaccacg ggccaggcgg atggcgctca tcgtcgcctc
    17101 ggtgccggag ttcaccatgc gcaccatatc catggtcggg accagttcgg tcaccagttg
    17161 cgccattttc acttccattt cggtcggtgc accaaagctt aaaccacgct cggcggcttc
    17221 aatcacggca ttgcggatcg ccggatggtt atggcccagc accatcggcc cccaggaacc
    17281 gacataatcg atataggctt tgccatcaac atcgtacaga taagcgccgt ccgctttttc
    17341 gataaacagt ggagtgccgc ccacgccagt aaaggcgcga acaggggagt tcacaccgcc
    17401 agggatcagc tcgcgcgctg cgctgtaaag attttcagac ttactcatgg agggttcctg
    17461 attcgtagaa aaagtgaatg gctgctattc catgttattc ataacaagtt aaatactcgt
    17521 caaacatcag gctgcttgta ctggtgcaat cctgatttct tagagtataa aagttttgtg
    17581 catttgaaac attacgcttt gcaaaggatt ttcatggaac gtgcgagtaa aatgccgtca
    17641 tcttatttgt atgaccaata agtgatcatt ggatgaaaac tgatactccc tctttagaaa
    17701 caccgcaggc cgcgcgcctg cgacgcagac aactgattcg ccaacttctt gagcgcgata
    17761 aaaccccgtt agccattttg tttatggcgg cagtcgtcgg cacgcttgtc gggctggctg
    17821 cggttgcttt tgacaaaggt gtcgcctggt tgcagaacca acgtatgggg gcgctggtac
    17881 atactgctga taattatccg ctactgttaa ccgtcgcttt tctctgttcg gcggtgctgg
    17941 cgatgttcgg ctactttttg gtgcgcaaat acgcgcccga agcaggggga tcggggatcc
    18001 cggaaattga aggggcgctg gaagatcaac gtcctgttcg ctggtggcgt gtattgccgg
    18061 tgaaattctt tggcgggctg gggacactcg gcggaggcat ggggctaggg cgcgaaggac
    18121 caaccgtgca gatcggcggc aatatcagtc gcatggtgct tgatattttc cgcctgaaag
    18181 gtgatgaagc tcgccatacg ctgctggcaa ccggtgctgc tgcggggctg gctgcggcct
    18241 ttaacgcgcc gctggcgggt attttgttta ttatcgaaga gatgcgtccg cagtttcgct
    18301 atacgttaat ttcgattaaa gcggtattta ttggcgtcat tatgtcgacc attatgtacc
    18361 ggatttttaa tcatgaagtt gcgttgattg acgtcggtaa actttctgat gcgccgctta
    18421 atacgctgtg gctttatctg atcctcggta ttatttttgg cattttcggc cctattttta
    18481 ataaatgggt gctggggatg caggatttgc tgcaccgtgt gcacggcggc aatattacca
    18541 aatgggtact gatggggggg gcgattggcg gtctgtgtgg attgctgggg tttgtggcac
    18601 cagcaacgtc gggcggcggt tttaacctga ttcctatcgc taccgcgggg aatttcagca
    18661 tgggaatgct ggtgtttatc ttcgtcgctc gggtcattac caccttactc tgcttctctt
    18721 ccggcgcgcc gggcggtatt tttgccccga tgctggcgct gggtactgtg ctgggaaccg
    18781 ctttcggaat ggttgccgtt gagctgtttc cgcaatatca cctggaggcg gggacgtttg
    18841 ctattgccgg aatgggggca ttactggcgg catctattcg cgcgccgtta acggggatca
    18901 ttctggttct ggagatgacc gataactacc agctcatttt gccaatgatt attaccggtc
    18961 ttggcgcaac actattagcg caatttaccg gcgggaaacc tctatactcg gcgattcttg
    19021 cgcgcacgct ggcaaaacag gaagctgagc aactggcgcg aagcaaggcc gcatcagcca
    19081 gcgagaatac ttgaacgaaa taccagggta ttagataatg gcgattatta ttgggttaga
    19141 atttgcccga ttgccgatgt cgtttggagc aaaatatgag tgatgacgta gcactgccgc
    19201 tggagtttac cgacgcagca gccaacaaag ttaaaagcct gatcgctgac gaagataacc
    19261 cgaatctgaa attacgcgtg tatatcaccg gtggcggttg cagcggcttc cagtatggtt
    19321 tcacctttga tgatcaggtg aacgaaggcg atatgaccat cgaaaaacag ggcgttggcc
    19381 tggtggttga tccgatgagc ctgcaatatc tggtcggcgg ttccgttgac tacaccgaag
    19441 gactggaagg ttctcgtttc atcgtgacca acccgaacgc gaaaagcacc tgcggttgcg
    19501 gttcttcctt tagcatctaa tctgttgttc atgtgccgga taaactatcc ggcacatctt
    19561 ctctcttcaa cgcccattct catccagcgc aaacgtcggt agcttaagat gccaacgaat
    19621 agccgccagc cgaatcaata gcgtcacgac catgcccatc atactggctg tttccagtgg
    19681 tacggaaaat gtgtaataag ccgtagcgtg gacaataccg ccgataatac aggcagttgc
    19741 gtagatttct gtacgtaaaa tcatggggat ttcgcggacc agtacatcac gaatgatccc
    19801 gccgccaacg ccagtaatga cgcccataca aaccgcgatt aacggaccgg cttccgcatt
    19861 aaaggcttta ttcacgctaa tgccgacaaa caccgccaga ccaacggcgt ccagcaccgg
    19921 caacatccat tttggtaagc gtcttggctg gcgcaccagc acgatggtca gcatgctggt
    19981 gaccatggca acgaccagat cggtgggatc tttcacccaa aataccgggc cgtgatccag
    20041 cgccatgtcg cgaattgttc cgtcgcctac tgcggtaacc acgcccagta ccagaacacc
    20101 aaaagggtcc atacgcaatt ttccggctaa caaaacgccg tagatggcaa ataccgctgt
    20161 gccgactata tccagccaat agacgagcat tgttcaatcc ccactgagca cctgaaaagg
    20221 tcaggcacta atctacctgt gaaagcgcat tacagagctg ttgtgctgcg aggataatac
    20281 gtgggcttgc acgttcaaac cagtcactcg tgagaggaat aacgggaatt ttgagctgtt
    20341 caccccagta ttgtttgatt ttaggaattt ggtccggtcc gcctgtaatg acaatagcct
    20401 gtggcgagcg tgctaacacc tgttcgcggc taacttgcgg ccagggaacc cggctgtctt
    20461 taaagatgtt ttctccgcca caaacttcga gtacctggtt ctgaatcgac tcttttccac
    20521 tggtaaatgg cggattaatg ccgaattgca gaaaaacacg ttttttaggt ttatcagcat
    20581 attgcgcttt caattgcacg tactgatcca gcagggattg cgcggcttgt tcggccttgt
    20641 ctggttgcgg actccagggg gccagttgac gtaacgcatt ggcaatttgt tcaatgcttg
    20701 tcgcatcgac ccacatcact tttattccca gcgaagccag ctggtcaacc tgccgctcgg
    20761 cattacctcc acgccaggca atcaccagat cgggtttcag cgcgacaatg cgttccagat
    20821 tcatcccctg ccaggtggaa acctgctcaa tcttttgcgc ttgtggagga tagtcggaat
    20881 agctgctgac cccaaccggc gtgatcccgg cggcaaaggc aagttcagtg ttggcgggag
    20941 aaagcgtgat gacgcgcggc gcggcgttga gccacagtgg cgcgagaaaa gacagggcga
    21001 ccagcgccct gaacagtgat ttagccatgt gccagtttct gcaccagtga ctcaaccatc
    21061 aggctggact gtttagcggc aacagccagg aactcatcga agctaagatg agactgttga
    21121 tcggccacgt cggagatggc gcgtacgaca acaaacggga cgttgaaatt gtggcagaca
    21181 tgggcgattg ccgtcgcttc catctctaca gcaatggcct gtgggaagtt gtggcggatt
    21241 ttcgccagac caacagaacc gttgatgaaa gcgtcgccgc taacaatcag gccacgtaca
    21301 gcgttaagat tcagttcggc aatgcaggcc tcagcggcag cgatcagttt atcgtcagct
    21361 ttaaagcctg ccggacagcc tggtaactga ccgtattcat aaccaaatgc cgtgacatcc
    21421 gcgtcgtgat aacgcgcttc gtccgagaca acgatatcgc ccactttcag cgttggtgcc
    21481 aggccaccgg cagaaccggt gttaataatc acatctggct tgcagtgttc caacagcaga
    21541 gtggcaccca gcgccgcagc gactttaccg atgcccgatt tcagaagcgc aacctcggtt
    21601 ccattcagtt ggccggtata gatttcgcaa ccgccgagac tgatagtttg acggttttcg
    21661 attttgtcac gcagcagcgt aacttcttct tccattgcac caatgatgcc gattttcata
    21721 gatttactcg cgataagccc gatttgaagg catagtttac catgcgctta cggggaagcg
    21781 tatttctcac gcgggagagg acatggcaca gattgatttc cgaaaaaaaa taaactggca
    21841 tcgtcgttac cgttcaccgc agggcgttaa aaccgaacat gagatcctgc ggatcttcga
    21901 gagcgatcgc gggcgtatca tcaactctcc ggcaattcgt cgtctgcaac aaaagaccca
    21961 ggtttttcca ctggagcgca atgccgccgt gcgcacgcgt cttacccact cgatggaagt
    22021 ccagcaggtg ggacgctaca tcgccaaaga aattttaagc cgtctgaaag agcttaaatt
    22081 gctggaagca tacggcctgg atgaactgac cggccccttt gaaagcattg ttgagatgtc
    22141 atgcctgatg cacgatatcg gcaatccgcc gtttggtcat tttggcgaag cagcgataaa
    22201 tgactggttt cgccaacgtt tgcacccgga agatgccgaa agccagcctc tgactgacga
    22261 tcgctgcagc gtggcggcac tacgtttacg ggacggggaa gaaccgctta acgagctgcg
    22321 gcgcaagatt cgtcaggact tatgtcattt tgaggggaat gcacaaggca ttcgtctggt
    22381 gcatacattg atgcggatga atctcacttg ggcacaggtt ggcggtattt taaaatatac
    22441 ccgtccggcg tggtggcgtg gcgaaacgcc tgagacacat cactatttaa tgaaaaagcc
    22501 gggttattat ctttctgaag aagcctatat tgcccgcttg cgtaaagaac ttaatttggc
    22561 gctttacagt cgttttccat taacgtggat tatggaagct gccgacgaca tctcctattg
    22621 tgtggcagac cttgaagatg cggtagagaa aagaatattt accgttgagc agctttatca
    22681 tcatttgcac gaagcgtggg gccagcatga gaaaggttcg ctcttttcgc tggtggttga
    22741 aaatgcctgg gaaaaatcac gctcaaatag tttaagccgc agtacggaag atcagttttt
    22801 tatgtattta cgggtaaaca ccctaaataa actggtaccc tacgcggcac aacgatttat
    22861 tgataatctg cctgcgattt tcgccggaac gtttaatcat gcattattgg aagatgccag
    22921 cgaatgcagc gatcttctta agctatataa aaatgtcgct gtaaaacatg tgtttagcca
    22981 tccggatgtc gagcagcttg aattgcaggg ctatcgggtc attagcggat tattagagat
    23041 ttatcgtcct ttattaagcc tgtcgttatc agactttact gaactggtag aaaaagaacg
    23101 ggtgaaacgt ttccctattg agtcgcgctt attccacaaa ctctcgacgc gccatcggct
    23161 ggcctatgtc gaggctgtca gtaaattacc gtcagattct cctgagtttc cgctatggga
    23221 atattattac cgttgccgcc tgctgcagga ttatatcagc ggtatgaccg acctctatgc
    23281 gtgggatgaa taccgacgtc tgatggccgt agaacaataa ccagtctttt gtaaagacga
    23341 acaataaatt tttacctttt gcagaaactt tagttcggaa cttcgggcta taaaacgaat
    23401 ctgaagaaca cagcaatttt gcgttatctg ttaatcgaga ctgaaataca tgaaaaaaaa
    23461 cacattagca ctgagtgcac tggctctgag tttaggtttg gcgttatctc cgctctctgc
    23521 aacggcggct gagacttctt cagcaacgac agcccagcag atgccaagcc ttgcaccgat
    23581 gctcgaaaag gtgatgcctt cagtggtcag cattaacgta gaaggtagca caaccgttaa
    23641 tacgccgcgt atgccgcgta atttccagca gttcttcggt gatgattctc cgttctgcca
    23701 ggaaggttct ccgttccaga gctctccgtt ctgccagggg ggccagggcg gtaatggcgg
    23761 cggccagcaa cagaaattca tggcgctggg ttccggcgtc atcattgatg ccgataaagg
    23821 ctatgtcgtc accaacaacc acgttgttga taacgcgacg gtgattaaag tccagctgag
    23881 cgatggtcgt aagttcgacg cgaagatggt tggcaaagat ccgcgctctg atatcgcgct
    23941 gatccaaatc cagaacccga aaaaactgac cgcaattaag atggcggatt ctgatgcact
    24001 gcgcgtgggt gattacaccg tagcgattgg taacccgttt ggtctgggcg agacggtaac
    24061 ttccgggatt gtctctgcgc tggggcgtag cggcctgaat gctgaaaact acgaaaactt
    24121 catccagacc gatgcagcga tcaaccgtgg taactccggt ggtgcgctgg ttaacctgaa
    24181 cggcgaactg atcggtatca acaccgcgat cctcgcaccg gacggcggca acatcggtat
    24241 cggttttgct atcccgagta acatggtgaa aaacctgacc tcgcagatgg tggaatacgg
    24301 ccaggtgaaa cgcggtgagc tgggtattat ggggactgag ctgaattccg aactggcgaa
    24361 agcgatgaaa gttgacgccc agcgcggtgc tttcgtaagc caggttctgc ctaattcctc
    24421 cgctgcaaaa gcgggcatta aagcgggtga tgtgatcacc tcactgaacg gtaagccgat
    24481 cagcagcttt gccgcactgc gtgctcaggt gggtactatg ccggtgggca gcaaactgac
    24541 cctgggctta ctgcgcgacg gtaagcaggt taacgtgaac ctggaactgc agcagagcag
    24601 ccagaatcag gttgattcca gctccatctt caacggcatt gaaggcgctg agatgagcaa
    24661 caaaggcaaa gatcagggcg tggtagtgaa caacgtgaag acgggcactc cggctgcgca
    24721 gatcggcctg aagaaaggtg atgtgattat tggtgcgaac cagcaggcag tgaaaaacat
    24781 cgctgaactg cgtaaagttc tcgacagcaa accgtctgtg ctggcactga acattcagcg
    24841 cggcgacagc accatctacc tgttaatgca gtaatctccc tcaacccctt cctgaatacg
    24901 ggaaggggtt ctccttacaa tctgtgaact tcgccacaac tccatacatc ttcatcatcc
    24961 tttgggcatt tgcacaatgc cgtacgttac gtacttcctt atgctaagcc gtgcataacg
    25021 gaggacttat ggctggctgg catcttgata ccaaaatggc gcaggatatc gtggcacgta
    25081 ccatgcgcat catcgatacc aatatcaacg taatggatgc ccgcgggcga attatcggca
    25141 gcggcgatcg tgagcgtatt ggtgaattgc acgaaggtgc attgctggta ctttcacagg
    25201 gacgagtcgt cgatatcgat gacgcggtag cacgtcatct gcacggtgtg cggcagggga
    25261 ttaatctacc gttacggctg gaaggtgaaa ttgtcggcgt aattggcctg acaggtgaac
    25321 cagagaatct gcgtaaatat ggcgaactgg tctgcatgac ggctgaaatg atgctggaac
    25381 agtcgcggtt gatgcacttg ttggcgcagg atagccgttt gcgggaagaa ctggtgatga
    25441 acctgattca ggcagaggag aatactcccg cacttctgaa tgggcgcaac ggctggggat
    25501 cgatctcaat caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt
    25561 ggacagcgca atggcggagt tacaacaact gcaaaacgcg ctgactacgc ccgagcgtaa
    25621 taatctggtg gcgattgtct cgctaaccga aatggtggtg ttgaaaccgg cgttgaactc
    25681 ttttgggcgc tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat
    25741 gaaagagtac ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg
    25801 cagtattgcc cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat
    25861 gccagaaagt cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt
    25921 gcgtggcgac tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga
    25981 caataacggc ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct
    26041 ggcaacgtca aaggcgttgt ttattcatcg taataccctg gagtatcggc ttaatcgtat
    26101 atcggaactg accgggcttg atttgggcaa ttttgatgac aggttgctgc tgtatgtggc
    26161 gttacaactg gatgaagagc ggtagattat gcgttaaggt tgaggcggcg ctggctcatt
    26221 cccctcagcc cgaaggggag aggggaatgc ccgagttaac cgtcaattcg ggattattta
    26281 cgcgttagtt tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc
    26341 aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctga
    26401 cagatttgat caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct
    26461 ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac
    26521 ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg
    26581 ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga
    26641 cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt
    26701 agcatttggc ttggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac
    26761 ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggcgata ttccatggga
    26821 tggattagca catttatctt cgttttgcga attacatgac agtccgaaac aagatgaaaa
    26881 agtatttaaa aaagggggag gaagaatgag taggacgaat tattctgagc cagaaagcat
    26941 acgggcagca tcatgctgcc cgtatatgta ctcttaatcg atggtacgca gcagttcgtt
    27001 aatgccgact ttgccgcgag ttttcgcgtc aactttctta acgataaccg cacagtagag
    27061 gctgtatttg ccatcttttg acggcagatt acctgaaaca accacagacc ccgccggaac
    27121 gcgaccgtag tggatttcgc cggtttcacg gtcgtaaata cgggtgctct gaccaatgta
    27181 tacgcccata gagatgacgg aaccttcttc gacaatcacc ccttcaacca cttcagagcg
    27241 tgcgccaatg aagcagttat cttcaataat ggtcgggtta gcctgcagcg gctccagcac
    27301 gccgccgatg cccacgccac cggaaaggtg gacgttttta ccaatctgcg cacaagaacc
    27361 gacggtcgcc caggtatcaa ccatggtgcc ttcatcaaca tatgcgccga tgttaacgta
    27421 agacggcatc agcacggtgt tacgggcaat aaacgcaccc tgacgtaccg ccgctggtgg
    27481 cacaacgcgg aagccttctt tctggaaacg tgcttcgtcg tagtcggcga atttcatcgg
    27541 cactttgtcg aagtagcggc tttctgcccc ttcgatcacc tgattatcat taatacggaa
    27601 agagagcagc accgcttttt tcaaccactg atgcgtcacc cactgaccgt caattttttc
    27661 cgctacacgc agtgcgccgg aatccagcag ggcgatcacc tgatttaccg cttcgcgggt
    27721 aacggtgtct gcattggctg gcgtgatctc ggcacggcgt tcaaaagcgg tttcaataat
    27781 gttctgtaac tgctgcattg ttaaactctt ttcatatcag taaacacatc accctttatc
    27841 gtttggattg agggcctctg tcaaccgctg atgcacttcc tgctgcaact cgttattaag
    27901 cgcacgccgg tcagcggtgg caattatgaa taaatcttct actcgctcgc caatggttgt
    27961 aattcgggca ccatgaagcg aaattcccag atcggcaaaa attttcccga ctcgcgccag
    28021 cagtccaggt tggtcgaggg cgatcagttc gaggaacgat ttgcggtcgg tatgggtcgg
    28081 caaaaacgtt acttcggttt caacagtaaa atggcgtaat ttggcgggtt ggcgacgggg
    28141 ctgcggtggc tgccagctac tttgcgtcag cacctgctcc agaccaaacc gaataacctc
    28201 atggcgatct gcggacagcg ggctgccatc gggttccagc acgataaagg tatccatcgc
    28261 cataccgtcg cgagtggtga aaatttgtgc gtcgtgaaca cttaaattgc ggcggtctaa
    28321 ttcggcacag acggcggcaa acagataggg gcggtccggg ctccagataa aaatctcggt
    28381 gcctccacgc gtagcctgcg ggctaagcaa taccagcggt ttgcttaaat catgctgtaa
    28441 taaatggcgg gcatgccagg ccagttgatt tgggctatgg cggacaaaat agttagcacg
    28501 acagcgtgac caaatttggt gcagcgcctc ttcgtcgatg ttatccatgc gcagtagtgc
    28561 cagtgcctgg agttggtgat ggcgaacccg ttcgcgcata tccggcgtgt tttgcattcc
    28621 gcgtcgtagc tgcttttcgg tggcaaaata gagttcgcgc aacagacttt gcttccagct
    28681 attccacagt gtttcgttgg tggcgcaaat gtcagccact gtcaggcata ccagatagcg
    28741 cagacgattt tccgtttgaa cttcttcggc aaactgcttg atgacttcag ggtcctgaat
    28801 atcgcggcgt tgtgctgtca ccgacatcag caagtgctgg cgaaccagcc aggcaaccag
    28861 ctgtgtttcg cgtgagttca gcccgtggag ttctgcaaaa tgcactacat cctgagcacc
    28921 gagaatggag tggtcgccgc cgcgtccttt ggcgatatcg tgaaacagcg cggcgatgaa
    28981 aatcagctca gttgacggca ggcgcggcca gacgtccaca cacaacggat ggcgctggcg
    29041 cgtttcttca ctggcaaaac tctccagttt cagcatcacg cggatagtat gttcatccac
    29101 cgtgtaggcg tggaacagat caaactgcat ctgcccgacg atatgcgacc attgcggcat
    29161 atacgcgccg agcacgctat ggcgatgcat tggcaatagt ccgcgccgca ccgctccggg
    29221 gtgacgcaga atgctcaaaa acagttttcg tgcttgcgga atattacaca gcggttgttg
    29281 cagatggcga cgggcatggc gtaactggcg cagcgtggtg gagtaaatgc cggtgatcgc
    29341 actgttgcgc accatggtgt agaacatacg caagatggct tccggctggc gcataaatag
    29401 tgtttcatca cgcaggtcga ttagcgtacc gcgtagctga aactcatcgt cgagtggacg
    29461 tggtttttcg tcggcgggaa gggcgaggat ggcttcatcg aacagttgca gcagcatctg
    29521 gttgagttca ctgacgcggc gtgtaacgcg gaagtaatcc ttcatcatcc gctcgaccgg
    29581 ttcgttgcct tcaccactgt aattcagacg ctgagcgacg ctaagttggc gatcgaataa
    29641 cagacgatta tcgtaacggc tgacgaccag atgcagggca aagcgaatac gccacagtat
    29701 atgcagacat tcgtttaatt ccgcccgctc cgctgaggtt aagaagccaa acccgaccat
    29761 ttcatccagc gatgttgcgc caaaatgacg gcgggccacc cattgcagag tgtgaatatc
    29821 gcgcaagccg ccagggctac ttttgatgtc tggttcaagg ttgtagctgg tgccatggta
    29881 acgctgatgg cgctggttct gttcttcaac tttcgctgcg tagaacttgt cggaaggcca
    29941 gaatccttcg ctaaaaatat gtttttgcag ttcgaggaag agcgcaacat cgccaattaa
    30001 taagcgggat tcgattaaat tggtggcgac ggttaaatcc gataaccctt ccaacatgca
    30061 ctcttcaagc gtgcgcacac tatgaccgac ttccagcttt acatcccaga gcagcgttaa
    30121 cagctcgccc actttttgcg cctgatcgtc cgggagcttt ttacggctta aaatcagtaa
    30181 atcgatgtca gaaagtggat gcagctcgcc acgaccgtag ccaccgacgg caaccaatgc
    30241 caggtcggca atctggctga atcccgcttc aatccataat cgttgcagga gctggtctat
    30301 gaactcggta cgtgcctcaa tcaactgttc tgcagagatc ccattgtcaa aggcatcacc
    30361 cagccaacgc tggaaagtat cgatatgggc ttttatccca cagacggtta attcatcacg
    30421 gggccaggcg catggatttt gcggttgacc gggcagggtg gagagagcgg tgtttgcgta
    30481 ctgttctgga agggtattca ttgtgcgcca cccataagat taaattatcg cattaaaaaa
    30541 gccggcattc gccggcttca tcttattcgt cgtgcgagat tatcgccggg atggtgtcat
    30601 ccttgcgtag cgtcaaaatt tcgcagccgt tatcagtcac cacaatagta tgctcatatt
    30661 gtgcagacaa gctgcgatct ttggttttta ccgtccagcc atctttcatg gtgcggatct
    30721 cttttttacc cgcgttgacc attggctcaa tggtgaacgt catccccggt ttcagcacga
    30781 cgttggtttc acgggagtca tagtgcagca cctgcggttc ttcatggaag ccgcgaccaa
    30841 taccgtgtcc gcaatattca cgaacgacgg agaagccttc tgcttcgaca aatttctgaa
    30901 tcgccgcacc gatttcgcgc agattaatgc ctggttttac catgcgtagc gccaggtaca
    30961 ggctttcttg cgtgatgcgg cacaggcgtt cgcccatgat ggtcggctta ccgacgataa
    31021 acattttcga ggtatcgccg tggaaaccat ctttgattac ggtgacatca atgttaacga
    31081 tatcgccatc tttcagcagc ttagcatcgt ccgggatacc gtggcacacc acttcattaa
    31141 tagagatgca aacggatttc ggatagccgt gatagccgag gcaggcagaa accgcgtgtt
    31201 gttcattaac aatgtaatca ttacagatgc gatccagctc gccggtgctg acgcccggtt
    31261 taacatacgg ttcgatcatc tccagcactt cggcagccag tcggccagcg acgcgcattt
    31321 tttcgatatc ttctggggtc ttgattgaga tagccattaa ttctgtccat cagcgtcggt
    31381 gataccgaca atatatatgt aagtgccgtc aatggtatca cacccgggca aattgagaat
    31441 cattctgaat ttcgccaaac gtgccactga aggttttcta taatagaaaa ttcgacgtct
    31501 gatgctgtac acagcgccaa caattattgg tgtccacgac gtatttgtgg tataaagcgc
    31561 gccggacttc cgatccattt cgtatacaca gactggacgg aagcgacaat ctcactttgt
    31621 gtaacaacac acacgtatcg gcacatattc cggggtgccc tttggggtcg gtaatatggg
    31681 atacgtggag gcataacccc aacttttata tagaggtttt aatcatggca actgtttcca
    31741 tgcgcgacat gctcaaggct ggtgttcact tcggtcacca gacccgttac tggaacccga
    31801 aaatgaagcc gttcatcttc ggtgcgcgta acaaagttca catcatcaac cttgagaaaa
    31861 ctgtaccgat gttcaacgaa gctctggctg aactgaacaa gattgtttct cgcaaaggta
    31921 aaatcctttt cgttggtact aaacgcgctg caagcgaagc ggtgaaagac gctgctctga
    31981 gctgcgacca gttcttcgtg aaccatcgct ggctgggcgg tatgctgact aactggaaaa
    32041 ccgttcgtca gtccatcaaa cgtctgaaag acctggaaac tcagtctcag gacggtactt
    32101 tcgacaagct gaccaagaaa gaagcgctga tgcgcactcg tgagctggag aaactggaaa
    32161 acagcctggg cggtatcaaa gacatgggcg gtctgccgga cgctctgttt gtaatcgatg
    32221 ctgaccacga acacattgct atcaaagaag caaacaactt gggtattccg gtatttgcta
    32281 tcgttgatac caactctgat ccggacggtg ttgacttcgt tatcccgggt aacgacgacg
    32341 caatccgtgc tgtgaccctg tacctgggcg ctgttgctgc aaccgtacgt gaaggccgtt
    32401 ctcaggatct ggcttcccag gcggaagaaa gcttcgtaga agctgagtaa taaggcttga
    32461 taactccccc aaaatagttc gagttgcaga aaggcggcaa gctcgagaat tcccgggagc
    32521 ttacatcagt aagtgaccgg gatgagcgag cgaagacaac gcatctgcgg cgcgaaatat
    32581 gaagggggag agcccttata gaccaggtag tacacgtttg gttagggggc ctgcatatgg
    32641 cccccttttt cacttttata tctgtgcggt ttaatgccgg gcagatcaca tctccgagga
    32701 ttttagaatg gctgaaatta ccgcatccct ggtaaaagag ctgcgtgagc gtactggcgc
    32761 aggcatgatg gattgcaaaa aagcactgac tgaagctaac ggcgacatcg agctggcaat
    32821 cgaaaacatg cgtaagtccg gtgctattaa agcagcgaaa aaagcaggca acgttgctgc
    32881 tgacggcgtg atcaaaacca aaatcgacgg caactacggc atcattctgg aagttaactg
    32941 ccagactgac ttcgttgcaa aagacgctgg tttccaggcg ttcgcagaca aagttctgga
    33001 cgcagctgtt gctggcaaaa tcactgacgt tgaagttctg aaagcacagt tcgaagaaga
    33061 acgtgttgcg ctggtagcga aaattggtga aaacatcaac attcgccgcg ttgctgcgct
    33121 ggaaggcgac gttctgggtt cttatcagca cggtgcgcgt atcggcgttc tggttgctgc
    33181 taaaggcgct gacgaagagc tggttaaaca catcgctatg cacgttgctg caagcaagcc
    33241 agaattcatc aaaccagaag acgtatccgc tgaagtggta gaaaaagaat accaggtaca
    33301 gctggatatc gcgatgcagt ctggtaagcc gaaagaaatc gcagagaaaa tggttgaagg
    33361 ccgcatgaag aaattcaccg gcgaagtttc tctgaccggt cagccgttcg ttatggaacc
    33421 aagcaaaact gttggtcagc tgctgaaaga gcataacgct gaagtgactg gcttcatccg
    33481 tttcgaagtg ggtgaaggca tcgagaaagt tgagactgac tttgcagcag aagttgctgc
    33541 gatgtccaag cagtcttaat tatcaaaaag gagccgcctg agggcggctt ctttttgtgc
    33601 ccatcttgta aattcagcta acccttgtgg ggctgcgctg aaaagcgacg tacaatgtcg
    33661 ctagtattaa ttcatttcaa tcgttgacag tctcaggaaa gaaacatggc taccaatgca
    33721 aaacccgtct ataaacgcat tctgcttaag ttgagtggcg aagctctgca gggcactgaa
    33781 ggcttcggta ttgatgcaag catactggat cgtatggctc aggaaatcaa agaactggtt
    33841 gaactgggta ttcaggttgg tgtggtgatt ggtgggggta acctgttccg tggcgctggt
    33901 ctggcgaaag cgggtatgaa ccgcgttgtg ggcgaccaca tggggatgct ggcgaccgta
    33961 atgaacggct tggcaatgcg tgatgcactg caccgcgcct atgtgaacgc tcgtctgatg
    34021 tccgctattc cattgaatgg cgtgtgcgac agctacagct gggcagaagc tatcagcctg
    34081 ttgcgcaaca accgtgtggt gatcctctcc gccggtacag gtaacccgtt ctttaccacc
    34141 gactcagcag cttgcctgcg tggtatcgaa attgaagccg atgtggtgct gaaagcaacc
    34201 aaagttgacg gcgtgtttac cgctgatccg gcgaaagatc caaccgcaac catgtacgag
    34261 caactgactt acagcgaagt gctggaaaaa gagctgaaag tcatggacct ggcggccttc
    34321 acgctggctc gtgaccataa attaccgatt cgtgttttca atatgaacaa accgggtgcg
    34381 ctgcgccgtg tggtaatggg tgaaaaagaa gggactttaa tcacggaata attcccgtga
    34441 tggataaata agggtaagat tccgcgtaag tatcgcgggg gcgtaagtct ggttataagg
    34501 cgttattgtt gcaggcagtt tggtcacggc cagcgcgcag caaccggagc gtacaaaagt
    34561 acgtgaggat ggcgagcact gctcggggcc aaaatggcaa ataaaatagc ctaataatcc
    34621 agacgattac ccgtaatatg tttaatcagg gctatactta gcacacttcc actgtgtgtg
    34681 actgtctggt ctgactgaga caagttttca aggattcgta acgtgattag cgatatcaga
    34741 aaagatgctg aagtacgcat ggacaaatgc gtagaagcgt tcaaaaccca aatcagcaaa
    34801 atacgcacgg gtcgtgcttc tcccagcctg ctggatggca ttgtcgtgga atattacggc
    34861 acgccgacgc cgctgcgtca gctggcaagc gtaacggtag aagattcccg tactctgaaa
    34921 atcaacgtgt ttgatcgttc aatgtctccg gccgttgaaa aagcgattat ggcctccgat
    34981 ctcggcctga acccgaactc tgcgggtagc gacatccgtg ttccgctgcc gccgctgacg
    35041 gaagaacgtc gtaaagatct gaccaaaatc gttcgtggtg aagcagaaca agcgcgtgtt
    35101 gcagtacgta atgtgcgtcg tgacgcgaac gacaaagtga aagcactgtt gaaagataaa
    35161 gagatcagcg aagacgacga tcgccgttct caggacgatg tacagaaact gactgatgct
    35221 gcaatcaaga aaattgaagc ggcgctggca gacaaagaag cagaactgat gcagttctga
    35281 tttcttgaac gacaaaaacg ccgctcagta gatccttgcg gatcggctgg cggcgttttg
    35341 ctttttattc tgtctcaact ctggatgttt catgaagcaa ctcaccattc tgggctcgac
    35401 cggctcgatt ggttgcagca cgctggacgt ggtgcgccat aatcccgaac acttccgcgt
    35461 agttgcgctg gtggcaggca aaaatgtcac tcgcatggta gaacagtgcc tggaattctc
    35521 tccccgctat gccgtaatgg acgatgaagc gagtgcgaaa cttcttaaaa cgatgctaca
    35581 gcaacagggg agccgcaccg aagtcttaag tgggcaacaa gccgcttgcg atatggcagc
    35641 gcttgaggag gttgatcagg tgatggcagc cattgttggt gctgctgggc tgttacctac
    35701 gcttgctgcg atccgcgcgg gtaaaaccat tttgctggcc aataaagaat cactggttac
    35761 ctgcggacgt ctgtttatgg acgccgtaaa gcagagcaaa gcgcaattgt taccggtcga
    35821 tagcgaacat aacgctattt ttcagagttt accgcaacct atccagcata atctgggata
    35881 cgctgacctt gagcaaaatg gcgtggtgtc cattttactt accgggtctg gtggcccttt
    35941 ccgtgagacg ccattgcgcg atttggcaac aatgacgccc gatcaagctt gccgtcatcc
    36001 gaactggtcg atgggacgta aaatttctgt cgattcggct accatgatga acaaaggtct
    36061 ggaatacatt gaagcgcgtt ggctgtttaa cgccagcgcc agccagatgg aagtgctgat
    36121 tcacccgcag tcagtgattc actcaatggt gcgctatcag gacggcagtg ttctggcgca
    36181 gctgggggaa ccggatatgc gtacgccaat tgcccacacc atggcatggc cgaatcgcgt
    36241 gaactctggc gtgaagccgc tcgatttttg caaactaagt gcgttgacat ttgccgcacc
    36301 ggattatgag cgttatccat gcctgaaact ggcgatggag gcgttcgaac aaggccaggc
    36361 agcgacgaca gcattgaatg ccgcaaacga aatcaccgtt gctgcttttc ttgcgcaaca
    36421 aatccgcttt acggatatcg ctgcgttgaa tttatccgta ctggaaaaaa tggatatgcg
    36481 cgaaccacaa tgtgtggacg atgtgttatc tgttgatgcg aacgcgagtg aagtcgccag
    36541 aaaagaggtg atgcgtctcg caagctgagg ataatccggc tacagagagt cgcgctattt
    36601 gttagcgtag ggcttcagtg atatagtctg cgccatctga tcgtaagtag ttggctttat
    36661 aaggtcaggt atgccgtggt tttacacggc ttttttttgt ataggcttca gtattcctga
    36721 gtaccgtaaa ccctgtcagg gaataaaaaa cgcgtgatgt tgtctgctac tcaaccactt
    36781 agcgaaaaat tgccagcgca tggctgccgt catgttgcga tcattatgga cggcaatggt
    36841 cgctgggcaa aaaagcaagg gaagattcgt gcctttgggc ataaagccgg ggcaaaatcc
    36901 gtccgccggg ctgtctcttt tgcggccaac aacggtattg aggcgttaac gctgtatgcc
    36961 tttagtagtg aaaactggaa ccgaccagcg caggaagtca gtgcgttaat ggaactgttt
    37021 gtgtgggcgc tcgatagcga agtaaaaagt ctgcaccgac ataacgtgcg tctgcgtatt
    37081 attggcgata ccagtcgttt taactcgcgt ttgcaagaac gtattcgtaa atctgaagcg
    37141 ctaacagccg gaaataccgg tctgacgctg aatattgcgg cgaactacgg tggacgttgg
    37201 gatatagtcc agggagtcag gcaactggct gaaaaggtgc agcaaggaaa cctgcaacca
    37261 gatcagatag atgaagagat gttaaaccag catgtctgta tgcatgaact ggcccctgta
    37321 gatttagtaa ttaggactgg gggagagcat cgcattagta actttttgct ttggcaaatt
    37381 gcctatgccg aactttactt tacagatgtt ctctggcccg atttcgatga acaagacttt
    37441 gaaggggcgt taaatgcctt tgctaatcga gagcgtcgtt tcggcggcac cgagcccggt
    37501 gatgaaacag cctgatgggg gtcgcttttg ctgaagtatc gcctgatatc tgcttttgtg
    37561 ttaatacccg tcgtcatcgc ggcgttgttt ctgttgccgc cggtggggtt cgccattgta
    37621 acgctggtgg tctgcatgct ggcagcgtgg gaatggggac agcttagcgg ttttaccact
    37681 cgttcgcagc gagtatggtt ggcggtgtta tgcgggttat tgttggcgct gatgcttttt
    37741 ctgttgccgg aatatcaccg aaatattcat caaccgctgg ttgaaatctc actttgggct
    37801 tcgttgggtt ggtggattgt cgcgctattg ctggtgctgt tttacccagg ttccgcagca
    37861 atctggcgta actctaaaac attgcgcctt atttttggcg tgctaaccat tgttcccttc
    37921 ttctggggca tgctggcgtt acgggcctgg cactatgacg agaatcatta cagtggcgca
    37981 atatggctgc tctatgtcat gatcctggta tggggcgctg actccggcgc atatatgttt
    38041 ggcaaattgt ttggtaaaca taagctggca ccgaaggttt ctccgggtaa aacctggcaa
    38101 ggctttatag gtggactcgc tactgcagcg gtaatctcat ggggttatgg catgtgggcg
    38161 aatctcgacg tcgctcccgt caccttactc atttgctcta ttgtcgcagc gttagcctca
    38221 gtgctcggcg atctgaccga gagtatgttt aagcgtgaag caggaattaa ggacagcggt
    38281 catttaattc caggacacgg tggtatttta gatcgtattg atagcctgac ggctgcggta
    38341 ccggtctttg cttgcttgtt gttactggta ttcaggacgc tttaacggaa ggtaatatgc
    38401 tgagttttct ctgggatttg gcttcgttca tcgttgcact gggtgtactt atcaccgtgc
    38461 atgaatttgg tcatttctgg gttgcccggc gttgtggtgt tcgcgttgag cgtttctcaa
    38521 tagggtttgg taaggcgctc tggcggcgaa ctgataagct cggcaccgaa tatgttatcg
    38581 ccctgatccc gttgggcggt tatgtcaaaa tgctggatga gcgcgcagaa ccggtcgttc
    38641 cggaactccg ccaccatgcc ttcaataata aatctgtcgg ccaacgagcg gcgattattg
    38701 ccgcaggtcc ggttgcaaac ttcatttttg ctatctttgc ctactggctg gtttttatta
    38761 ttggtgtgcc tggcgtacgt ccggtggttg gcgaaatagc agccaattcg atagctgcgg
    38821 aagcacaaat tgcaccaggt acggaactaa aagccgtaga tggtatcgaa acgcctgatt
    38881 gggatgccgt gcgtttgcag ttggtcgata aaattggcga tgaaagcacc accattacag
    38941 tagcgccatt tgacagcgac caacggcggg atgtaaagct cgatttacgt cactgggcgt
    39001 ttgagcctga taaagaagat ccggtatctt cgctggggat tcgtcctcgt gggccgcaaa
    39061 ttgaacctgt actggaaaat gtgcagccaa actcggcggc aagcaaggca ggtttgcaag
    39121 caggcgacag gatcgttaaa gtcgatggtc agcccttaac gcagtgggtg acctttgtga
    39181 tgcttgtccg ggataacccg ggtaaatcct tagcgttaga aatcgaaagg caggggagtc
    39241 ccttgtcttt gacattaatc ccggagagta aaccgggtaa tggtaaagcg attggttttg
    39301 tcggtattga gccgaaagtc attcctttgc cagatgagta taaagttgta cgccagtatg
    39361 ggccgttcaa cgccatcgtc gaagccacgg acaaaacgtg gcagctgatg aagctgacgg
    39421 tcagtatgct gggaaaattg atcaccggtg atgtgaaact gaacaacctc agtgggccga
    39481 tctctatcgc caagggggct gggatgacag cggaactcgg ggtagtttat tacctgccgt
    39541 ttcttgcgct tattagcgtg aacttaggga taattaacct gtttccgttg cccgtacttg
    39601 acggggggca tctgctgttc cttgcgatcg aaaagatcaa gggcggaccg gtatccgagc
    39661 gggttcaaga cttttgttat cgcattggct cgattctgct ggtgctgtta atggggcttg
    39721 cacttttcaa tgatttctct cggttatgag agttagttag gaagaacgca taataacgat
    39781 ggcgatgaaa aagttgctca tagcgtcgct gctgtttagc agcgccaccg tatacggtgc
    39841 tgaagggttc gtagtgaaag atattcattt cgaaggcctt cagcgtgtcg ccgttggtgc
    39901 ggccctcctc agtatgccgg tgcgtacagg cgacacggtt aatgatgaag atatcagtaa
    39961 taccattcgc gctctgtttg ctaccggcaa ctttgaggat gttcgcgtcc ttcgtgatgg
    40021 tgataccctt ctggttcagg taaaagaacg tccgaccatt gccagcatta ctttctccgg
    40081 taacaaatcg gtgaaagatg acatgctgaa gcaaaacctc gaggcttctg gtgtgcgtgt
    40141 gggcgaatcc ctcgatcgca ccaccattgc cgatatcgag aaaggtctgg aagacttcta
    40201 ctacagcgtc ggtaaatata gcgccagcgt taaagctgtc gtgaccccgc tgccgcgcaa
    40261 ccgtgttgac ctaaaactgg tgttccagga aggtgtgtca gctgaaatcc agcaaattaa
    40321 cattgttggt aaccatgctt tcaccaccga cgaactgatc tctcatttcc aactgcgtga
    40381 cgaagtgccg tggtggaatg tggtaggcga tcgtaaatac cagaaacaga aactggcggg
    40441 cgatcttgaa accctgcgca gctactatct ggaccgcggt tatgcccgtt tcaacatcga
    40501 ctctacccag gtcagtctga cgccagataa aaaaggtatt tacgtcacgg tgaacatcac
    40561 cgaaggcgat cagtacaagc tttctggcgt tgaagtgagc ggcaaccttg ccgggcactc
    40621 cgctgaaatt gagcaactga ctaagatcga gccgggcgag ctgtataacg gcactaaagt
    40681 gaccaaaatg gaagatgaca ttaaaaagct tctcggtcgc tatggttatg cctatccgcg
    40741 cgtacagtcg atgcctgaaa ttaacgatgc cgacaaaacc gttaaattac gcgtgaacgt
    40801 tgatgcgggt aaccgtttct acgtgcgtaa gatccgtttt gaaggtaacg atacctcgaa
    40861 agatgccgtc ctgcgtcgcg aaatgcgtca gatggaaggt gcatggttgg ggagcgatct
    40921 ggtcgatcag ggtaaggagc gtctgaatcg tctgggcttc tttgaaactg tcgataccga
    40981 tacccaacgt gttccgggta gcccggacca ggttgatgta gtctacaagg tgaaagaacg
    41041 taacactggt agcttcaact tcgggattgg ttacggtact gaaagtggcg tgagcttcca
    41101 ggctggcgtg caacaggata actggttagg tacaggttat gctgttggta tcaacgggac
    41161 caaaaacgat taccagacct atgctgaact gtcggtaacc aacccgtact tcaccgtaga
    41221 tggcgtaagc ctcggtggtc gtctcttcta taatgacttc caggcagatg acgccgacct
    41281 gtccgactat accaacaaga gttatggtac agacgtgacg ttgggcttcc cgattaacga
    41341 atataactcg ctgcgtgcag gtctgggtta tgtacataac tccctgtcca acatgcagcc
    41401 tcaggttgcg atgtggcgtt atctgtactc tatgggtgaa catccgagca cctctgatca
    41461 ggataacagc ttcaaaacgg acgacttcac gttcaactat ggttggacct ataacaagct
    41521 tgaccgtggt tacttcccga cagatggttc acgtgtcaac ctgaccggta aagtgaccat
    41581 tcctggatcg gataacgaat actacaaagt gacgttagac acggcgactt atgtgccgat
    41641 cgatgacgat cacaaatggg ttgttctggg tcgtacccgc tggggttatg gtgatggttt
    41701 aggcggcaaa gagatgccgt tctacgagaa cttctatgcc ggtggttcca gcaccgtgcg
    41761 tggcttccag tccaatacca ttggtccgaa agcagtttac ttcccgcatc aggccagtaa
    41821 ttatgatccg gactatgatt acgaatgtgc gactcaggac ggcgcgaaag acctgtgtaa
    41881 atcggatgat gctgtaggcg gtaacgccat ggcggttgcc agcctcgagt tcatcacccc
    41941 gacgccgttt attagcgata agtatgctaa ctcggttcgt acttcattct tctgggatat
    42001 gggtaccgtt tgggatacaa actgggattc cagccaatat tctggatatc cggactatag
    42061 tgatccaagc aatatccgta tgtctgcggg tatcgcatta caatggatgt ccccattggg
    42121 gccgttggtg ttctcctacg cccagccgtt caaaaagtac gatggagaca aggcagaaca
    42181 gttccagttt aacatcggta aaacctggta agtgttctcc acaaaggaat gtagtggtag
    42241 tgtagcgatg actttaggcg atcaatataa gatcgccggg ccacgcaaag aactgcaccc
    42301 tccggtgcaa atgggatggt aaggagttta ttgtgaaaaa gtggttatta gctgcaggtc
    42361 ttggtttagc actggcaact tctgctcagg cggctgacaa aattgcaatc gtcaatatgg
    42421 gcagcctgtt ccagcaggta gcgcagaaaa ccggtgtttc taacacgctg gaaaatgagt
    42481 tcaaaggccg tgccagcgaa ctgcagcgta tggaaaccga tctgcaggct aaaatgaaaa
    42541 aactgcagtc catgaaagcg ggcagcgatc gcaccaaact ggaaaaagac gtgatggctc
    42601 agcgccagac ttttgctcag aaagcgcagg cttttgagca ggatcgcgca cgtcgttcca
    42661 acgaagaacg cggcaaactg gttactcgta tccagactgc tgtgaaatcc gttgccaaca
    42721 gccaggatat cgatctggtt gttgatgcaa acgccgttgc ttacaacagc agcgatgtaa
    42781 aagacatcac tgccgacgta ctgaaacagg ttaaataagt aatgccttca attcgactgg
    42841 ctgatttagc gcagcagttg gatgcagaac tacacggtga tggcgatatc gtcatcaccg
    42901 gcgttgcgtc catgcaatct gcacaaacag gtcacattac gttcatggtt aacccaaaat
    42961 accgtgagca tttaggcttg tgccaggcgt ccgcggttgt catgacccag gacgatcttc
    43021 ctttcgcgaa aagtgccgcg ctggtagtga agaatcccta cctgacttac gcgcgcatgg
    43081 cgcaaatttt agataccacg ccgcagcccg cgcagaacat tgcacccagt gcggtgatcg
    43141 acgcgacggc gaagctgggt aacaacgtat cgattggcgc taacgcggtg attgagtccg
    43201 gcgttgaact gggcgataac gtgattatcg gtgccggttg cttcgtaggt aaaaacagca
    43261 aaatcggtgc aggttcgcgt ctctgggcga acgtaaccat ttaccatgag atccagatcg
    43321 gtcagaattg cctgatccag tccggaacag tggtaggcgc agacggcttt ggttatgcca
    43381 acgatcgtgg taactgggtg aagatcccac agattggtcg cgtaattatt ggcgatcgcg
    43441 tggagatcgg tgcctgcaca accatcgatc gcggcgcgct ggatgacact gttattggca
    43501 atggcgtgat cattgataac cagtgccaga ttgcacataa cgtcgtgatt ggcgacaata
    43561 cggcggttgc cggtggcgtc attatggcgg gcagcctgaa aattggtcgt tactgcatga
    43621 tcggcggagc cagcgtaatc aacgggcata tggaaatatg cgacaaagtg acggttacgg
    43681 gcatgggtat ggtgatgcgt cccatcactg aaccaggcgt ctattcctca ggcattccgc
    43741 tgcaacccaa caaagtctgg cgcaaaaccg ctgcactggt gatgaacatt gatgacatga
    43801 gcaagcgtct gaaatcgctt gagcgcaagg ttaatcaaca agactaacgt tccatctttt
    43861 gttcgccaaa ctttacggcc tgtctcattc ttacgattgc ggcaggccgt gttattattg
    43921 tcgtttctta tattttgaca ggaagagtat cttgactact aacactcata ctctgcagat
    43981 tgaagagatt ttagaacttc tgccgcaccg tttcccgttc ttactggtgg atcgcgtgct
    44041 ggattttgaa gaaggtcgtt ttctgcgcgc agtaaaaaat gtctctgtca atgagccatt
    44101 cttccagggc catttccctg gaaaaccgat tttcccgggt gtgctgattc tggaagcaat
    44161 ggcacaggca acaggtattc tggcgtttaa aagcgtagga aaactggaac cgggtgagct
    44221 gtactacttc gctggtattg acgaagcgcg cttcaagcgc ccggtcgtgc ctggcgatca
    44281 aatgatcatg gaagtcactt tcgaaaaaac gcgccgcggc ctgacccgtt ttaaaggggt
    44341 tgctctggtc gatggtaaag tagtttgcga agcaacgatg atgtgtgctc gtagccggga
    44401 ggcctgatac gtgattgata aatccgcctt tgtgcatcca accgccattg tggaagaggg
    44461 cgcgtcaatt ggcgcgaacg cacacattgg tcctttttgt atcgttggac cccatgtcga
    44521 aattggtgag ggtaccgtac tgaaatctca cgttgtcgtg aatggtcata ctaaaattgg
    44581 ccgcgataat gagatttatc agtttgcctc catcggcgaa gttaaccagg atttgaaata
    44641 tgctggcgaa ccgacccgtg tggaaatcgg cgatcgtaac cgcattcgcg aaagcgtcac
    44701 cattcatcgt ggcacagtcc agggcggtgg attgacgaag gtgggcagcg acaacttact
    44761 gatgatcaac gcgcacattg cgcacgattg tacggtaggt aaccgctgta ttctcgccaa
    44821 caacgcaacg ctggcgggtc acgtatcggt tgacgacttc gcgatcatcg gcggcatgac
    44881 cgcagtccat cagttctgca tcattggtgc gcatgtgatg gttggcggct gctccggtgt
    44941 ggcgcaggac gtccctcctt atgttattgc gcagggtaac cacgcaacgc cgttcggtgt
    45001 caatatcgaa gggctgaagc gccgcggatt cagccgtgag gcgattaccg ctatccgcaa
    45061 tgcgtataag ctaatttatc gtagcggtaa aacgctcgat gaagtgaaac cggaaattgc
    45121 tgaactggcg gaaacatatc cggaagtgaa agcctttacc gatttctttg cacgctcaac
    45181 gcgcggtctg attcgttaat gactgaccag cgtccattaa cgattgccct ggtcgccgga
    45241 gaaacctccg gcgatatcct gggggccggt ttaatccgcg ctctgaaaga acgtgtgccc
    45301 aacgcccgct ttgttggtgt tgccgggcca cgaatgcagg ctgaaggctg cgaagcctgg
    45361 tacgaaatgg aagaactggc ggtgatgggc attgttgaag tgctcggtcg tctgcgtcgc
    45421 ttactgcata ttcgtgccga tctgacaaag cgttttggcg aactgaagcc agatgttttt
    45481 gttggtattg atgcgcccga cttcaatatt actcttgaag gtaacctcaa aaagcagggt
    45541 atcaaaacca ttcattacgt cagtccatcc gtctgggcgt ggcgacagaa acgcgttttc
    45601 aaaataggca gagccaccga tctggtgctc gcatttctgc ctttcgaaaa agcgttttat
    45661 gacaaataca atgtaccgtg ccgctttatc ggtcatacca tggctgatgc catgccgtta
    45721 gatccagata aaaatggtgc ccgtgatgtg ctggggatcc cttacgatgc ccactgtctg
    45781 gcattgttgc cgggcagccg tggtgcagaa gtcgaaatgc ttagtgccga tttcttgaaa
    45841 actgcccagc ttttgcgcca gacatatccg gatctcgaaa tcgtggtgcc gctggtgaat
    45901 gccaaacgcc gcgagcagtt tgaacgcatc aaagctgaag tcgcgccaga cctgtcggtt
    45961 catttgctgg atggaatggg ccgtgaggcg atggtcgcca gcgatgcggc actactggcg
    46021 tcggggacgg cagccctgga gtgtatgctg gcgaaatgcc cgatggtggt gggatatcgc
    46081 atgaagcctt ttaccttctg gttggcgaag cggctggtga aaactgatta tgtctcgctg
    46141 ccaaatctgc tggcgggcag agagttagtc aaagagttat tgcaggaaga gtgtgagccg
    46201 caaaaactgg ctgcggcgct gttaccgctg ttggcgaacg ggaaaaccag ccacgcgatg
    46261 cacgatacct tccgtgaact gcatcagcag atccgctgca atgccgatga gcaggcggca
    46321 caagccgttc tggagttagc acaatgatcg aatttgttta tccgcacacg cagctggttg
    46381 cgggtgtgga tgaagtcgga cgcgggccgt tagttggcgc ggtcgtcacc gctgcggtga
    46441 tccttgaccc ggcgcgcccg attgccgggc tgaatgattc caaaaagctg agcgaaaaac
    46501 gccgtctggc gctctatgaa gagatcaaag agaaagcgtt gagctggagt ctgggccgcg
    46561 cggaacccca cgaaatcgac gagctgaaca ttcttcatgc gaccatgctg gcgatgcagc
    46621 gtgccgtcgc tgggctgcat attgcgccgg aatatgtgtt gattgatggt aaccgctgcc
    46681 cgaaattacc gatgcctgcg atggctgtgg tgaaagggga tagccgcgta ccagaaatca
    46741 gtgccgcgtc tatcctggcg aaagtgacgc gtgacgccga aatggcggcg ctggatattg
    46801 ttttcccgca atatggtttt gcccaacata aagggtatcc aaccgctttt catctggaaa
    46861 aactggctga atatggcgcg actgaacatc atcgtcgcag ctttgggcct gtcaaacgcg
    46921 cactgggact tgcgtcctga ttcttgtgtc gagattaagt aaaccggaat ctgaagatgt
    46981 ctgaaccacg tttcgtacac ctgcgggtgc acagcgacta ctcgatgatc gatggcttgg
    47041 ccaaaaccgc accgctggta aaaaaggcgg cggcgttggg tatgccagca ctggcgatca
    47101 ccgatttcac caacctttgc ggtctggtga agttctacgg agcgggacat ggcgcaggga
    47161 ttaagcctat cgtcggggca gattttaatg tccagtgcga cctgctgggt gatgagttaa
    47221 cccacctgac ggtactggcg gcgaacaata ccggctatca gaatctggcg ttgctgatct
    47281 caaaagcgta tcagcgcggg tacggtgccg ccgggccgat catcgatcgc gactggctta
    47341 tcgaattaaa cgaagggttg atccttcttt ccggcgggcg catgggcgac gtcggacgca
    47401 gtcttttgcg tggtaacagc gcgctggtag atgagtgtgt cgcgttttat gaagaacact
    47461 tcccggatcg ctattttctc gagctgatcc gcaccggcag gccggatgaa gaaagctatc
    47521 tgcacgcggc ggtggaactg gcggaagcgc gcggtttgcc cgtcgtggcg actaacgatg
    47581 tgcgctttat cgacagcagc gactttgacg cacacgaaat ccgcgtcgcg atccacgacg
    47641 gctttaccct cgacgatcct aaacgcccgc gtaactattc gccgcagcaa tatatgcgta
    47701 gcgaagagga gatgtgtgag ctgtttgccg acatcccaga agcccttgcc aacaccgttg
    47761 agatcgccaa acgctgtaac gtaactgtgc gtcttggtga atacttcctg ccgcagttcc
    47821 cgaccgggga catgagcacc gaagattatc tggtcaagcg tgcaaaagaa ggcctggaag
    47881 agcgtctggc atttttattc cctgacgagg aagaacgtct taaacgccgc ccggaatatg
    47941 acgaacgtct ggagactgaa cttcaggtta tcaaccagat gggcttcccg ggctacttcc
    48001 tcatcgttat ggaatttatc cagtggtcga aagataacgg cgtaccggta gggccaggcc
    48061 gtggctccgg tgcgggttca ctggtggcct acgcgctgaa aatcaccgac ctcgatccgc
    48121 tggaatttga cctgctgttc gaacgtttcc ttaacccgga acgtgtctcc atgcctgact
    48181 tcgacgttga cttctgtatg gagaaacgcg atcaggttat cgaacacgtg gcggacatgt
    48241 acggtcgtga tgcggtatcg cagattatca ccttcggtac gatggcggcg aaagcggtga
    48301 tccgcgacgt aggccgcgtg ctggggcatc cgtacggctt tgtcgaccgt atctcgaaac
    48361 tgatcccgcc cgatccgggg atgacgctgg cgaaagcgtt tgaagccgag ccgcagctgc
    48421 cggaaatcta cgaagcggat gaagaagtta aggcgctgat cgacatggcg cgcaaactgg
    48481 aaggggtcac ccgtaacgcc ggtaagcacg ccggtggggt ggttatcgcg ccgaccaaaa
    48541 ttaccgattt tgcgccgctt tattgcgatg aagagggcaa acatccggtc acccagtttg
    48601 ataaaagcga cgttgaatac gccggactgg tgaagttcga cttccttggt ttgcgtacgc
    48661 tcaccatcat caactgggcg ctggagatga tcaacaagcg gcgggcgaag aatggcgagc
    48721 caccgctgga tatcgccgcg atcccgctgg atgacaagaa aagcttcgac atgctgcaac
    48781 gctcggaaac cacggcggta ttccagcttg aatcgcgcgg catgaaggac ctgatcaagc
    48841 gtctgcaacc tgactgcttc gaagatatga tcgcactggt ggcactgttc cgccctggtc
    48901 cgttgcagtc agggatggtg gataacttta tcgaccgtaa acatggtcgt gaagagatct
    48961 cctatccgga cgtgcagtgg cagcatgaaa gcctgaaatc ggtactggag ccaacctacg
    49021 gcattatctt gtatcaggaa caggtcatgc agattgccca ggtgctttct ggttataccc
    49081 tcggtggcgc ggatatgctg cgccgtgcga tgggtaagaa aaagccggaa gagatggcta
    49141 agcagcgttc tgtatttgct gaaggtgcag aaaagaacgg aatcaacgcc gaactggcga
    49201 tgaaaatctt cgacctggtg gagaaattcg cgggttacgg atttaacaaa tcgcactctg
    49261 cggcctatgc tttggtgtca tatcaaacgt tatggctgaa agcgcactat ccggcggagt
    49321 ttatggcggc ggtaatgacc gccgatatgg acaacaccga gaaggtggtg ggcctggtgg
    49381 atgagtgctg gcggatgggg ctgaaaatcc tgccaccaga tataaactcc ggtctttacc
    49441 atttccacgt caacgacgat ggcgaaatcg tgtatggtat tggcgccatc aaaggggtag
    49501 gtgaaggtcc gattgaggcc atcatcgaag cccgtaataa aggcggctac ttccgcgaac
    49561 tgtttgatct ctgcgcccgt accgacacca aaaagttaaa ccggcgggtg ctggaaaaac
    49621 tgatcatgtc cggggcgttt gaccgtcttg ggccacaccg cgcggcgctg atgaactcgc
    49681 tgggtgatgc gttaaaagcg gcagatcaac acgcgaaagc ggaagctatc ggtcaggccg
    49741 atatgttcgg cgtgctggcc gaagagccgg aacaaattga acaatcctac gccagctgcc
    49801 aaccgtggcc ggagcaggtg gtattagatg gggaacgtga aacgttaggc ctgtacctga
    49861 ccggacaccc tatcaaccag tatttaaaag agattgagcg ttatgtcgga ggcgtaaggc
    49921 tgaaagacat gcacccgaca gaacgtggta aagtcatcac ggctgcgggg ctcgttgttg
    49981 ccgcgcgggt tatggtcacc aagcgcggca atcgtatcgg tatctgcacg ctggatgacc
    50041 gttccgggcg gctggaagtg atgttgttta ctgacgccct ggataaatac cagcaattgc
    50101 tggaaaaaga ccgcatactt atcgtcagcg gacaggtcag ctttgatgac ttcagcggtg
    50161 ggcttaaaat gaccgctcgc gaagtgatgg atattgacga agcccgggaa aaatatgctc
    50221 gcgggcttgc tatctcgctg acggacaggc aaattgatga ccagctttta aaccgactcc
    50281 gtcagtctct ggaaccccac cgctctggga caattccagt acatctctac tatcagaggg
    50341 cggatgcacg cgcgcggttg cgttttggcg cgacgtggcg tgtctctccg agcgatcgtt
    50401 tattaaacga tctccgtggc ctcattggtt cggagcaggt ggaactggag tttgactaat
    50461 acaggaatac tatgagtctg aatttccttg attttgaaca gccgattgca gagctggaag
    50521 cgaaaatcga ttctctgact gcggttagcc gtcaggatga gaaactggat attaacatcg
    50581 atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact gacgcgtaaa atcttcgccg
    50641 atctcggtgc atggcagatt gcgcaactgg cacgccatcc gcagcgtcct tataccctgg
    50701 attacgttcg cctggcattt gatgaatttg acgaactggc tggcgaccgt gcgtatgcag
    50761 acgataaagc tatcgtcggt ggtatcgccc gtctcgatgg tcgtccggtg atgatcattg
    50821 gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg taactttggt atgccagcgc
    50881 cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc tgaacgcttt aagatgccta
    50941 tcatcacctt tatcgacacc ccgggggctt atcctggcgt gggcgcagaa gagcgtggtc
    51001 agtctgaagc cattgcacgc aacctgcgtg aaatgtctcg ccttggcgta ccggtagttt
    51061 gtacggttat cggtgaaggt ggttctggcg gtgcgctggc gattggcgtg ggcgataaag
    51121 tgaatatgct gcaatacagc acctattccg ttatctcgcc ggaaggttgt gcgtccattc
    51181 tgtggaagag cgccgacaaa gcgccgctgg cggctgaagc gatgggtatc atcgctccgc
    51241 gtctgaaaga gctgaaactg atcgactcca tcatcccgga accgctgggc ggcgctcacc
    51301 gtaacccgga agcgatggcg gcatcgttga aagcgcaact gctggcggat ctggccgatc
    51361 tcgatgtgtt aagcactgaa gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg
    51421 gttacgcgta attcgcaaaa gttctgaaaa agggtcactt cggtggccct tttttatcgc
    51481 cacggtttga gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat
    51541 tgccattatg ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc
    51601 ggcgctggtg gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa
    51661 atttatcgaa cataaccctc gaatttgcgg cgtgattttt gactgggatg agtacagtct
    51721 cgatttatgc agcgatatca atcaacttaa tgaatatctc ccgctttatg ccttcatcaa
    51781 cacccactcg acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga
    51841 atatgcgctg gggcaggcgg aagatatcgc cattcgtatg cgtcagtaca ccaacgaata
    51901 tcttgataac attacgccgc cgttcacgaa agccttgttt acctacgtca aagagcggaa
    51961 gtacaccttt tgtacgccgg ggcatatggg cggcaccgca tatcaaaaaa gcccggttgg
    52021 ctgtctgttt tatgattttt tcggcgggaa tacccttaag gctgatgtct ctatttcggt
    52081 caccgagctt ggttcgttgc ttgaccacac cgggccacac ctggaagcgg aagagtacat
    52141 cgcgcggact tttggcgcgg aacagagtta tatcgttacc aacggaatat cgacgtcgaa
    52201 caaaattgtg ggtatgtacg ccgcgccatc cggcagtacg ctgttgatcg accgcaactg
    52261 ccataaatca ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc
    52321 gacgcgtaat gcgttgggga ttctgggggg gatcccgcgc cgtgaattta ctcgcgacag
    52381 catcgaagag aaagtcgctg ccactacgca agcacaatgg ccggttcatg cggtgatcac
    52441 caactccacc tatgatggtt tgctctacaa caccgactgg atcaaacaga cgctggatgt
    52501 cccgtcgatc cacttcgatt ccgcctgggt gccgtatact cattttcatc caatctacca
    52561 gggtaaaagt ggtatgagcg gcgagcgtgt tgcgggaaaa gttttcttcg aaacgcaatc
    52621 gacccacaaa atgctggcgg cgttatcgca ggcttcactg atccacatta aaggcgagta
    52681 tgacgaagag gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc
    52741 cattgttgct tcggttaaga cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct
    52801 gattaaccgt tcggtggagc gggcgctgca ttttcgcaaa gaggtccagc ggctgcggga
    52861 agagtctgac ggttggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg
    52921 caggcccgtt gcgcctggcg aacaatggca cggctttaac gatgcggatg ccgatcatat
    52981 gtttctcgat ccggttaaag tcactatttt gacaccgggg atggacgaac agggcaatat
    53041 gagtgaggag gggatcccgg cggcgctggt agcaaaattc ctcgacgaac gtgggatcgt
    53101 agtagagaaa accggccctt ataacctgct gtttctcttt agtattggca tcgataaaac
    53161 caaagcaatg ggattattgc gtgggttgac ggaatttaag cgctcttacg atctcaacct
    53221 gcggatcaaa aatatgctgc ccgatctcta tgctgaagat cccgatttct accgcaatat
    53281 gcgtattcag gatctggcgc aagggatcca taagctgatt cgtaaacacg atctttccgg
    53341 tttgatgttg cgggcattcg atactttgcc ggaaatgatc atgacgccac atcaggcatg
    53401 gcagagacag attaaaggtg aagtagaaac cattgcgctg gaacaactgg tcggtagagt
    53461 atcggcaaat atgatcctgc cttatccacc gggcgtaccg ctgttgatgc caggagaaat
    53521 gctgaccaaa gagagccgca cggtactcga ttttctactg atgctttgtt ccgtagggca
    53581 acattacccc ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggcgttta
    53641 ccgcgtacga gtcctaaaaa tggcgggata acttgccaga gcggcttccg ggcgagtaac
    53701 gtgctgttaa caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg
    53761 attattgcga cggattatgc ggtgagcaaa gctttctact gcgatattct tggtttcacg
    53821 ctgcaaagcg aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat
    53881 gggcaatatg tgattgagct tttctctttt ccgttcccgc cggagcgccc cagccgaccg
    53941 gaagcttgcg gcctgcgtca tctggctttt agcgttgatg acatcgatgc ggcagtggcg
    54001 caccttgaaa gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa
    54061 cgcttcacct tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc
    54121 ttgtcatcgc cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat
    54181 catgacactc acgctcaata gacaacttct cacctcacgc cagattctgg tggcctttag
    54241 cggcgggctt gactccaccg ttctgctgca tcagttggtg cagtggcgga cggaaaatcc
    54301 gggcgtcact ctgcgcgcta ttcatgtgca tcacggttta agtgccaatg ccgatgcctg
    54361 ggttacgcat tgcgaaaacg tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt
    54421 acaacttgcg caagaggggc tggggattga ggcccaggcg cggcaggcac gttatcaggc
    54481 atttgcccgt accctattgc ccggcgaagt gctggtcacc gcgcaacatc tcgacgatca
    54541 atgtgaaacc tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat
    54601 ggcggaagtt tcggagtttg ctggaacacg gcttatccgc ccgttgcttg cccgcacgcg
    54661 gggggaactg gagcagtggg cattggcgca tggtttacgc tggattgaag acgaaagtaa
    54721 tcaggacgac tcatacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca
    54781 gcgttggccg cattttgcgg aagcaacggc ccgcagcgcc acactttgtg ctgaacaaga
    54841 gagtctgttg gatgaactgc tggcggatga tttagcacac tgccaaacgt cgcaggggac
    54901 gctgcagatt gcgccaatgc tggcgatgag tgatgcccgt cgcgcggcga ttattcgccg
    54961 ctggctggca gggcagaatg caccgatgcc ttcccgcgac gcgctggtgc gcatctggca
    55021 ggaagtggcg ctggcgcggg aagatgcttc cccctgttta cgtttgggcg cctttgaaat
    55081 ccgccgctat cagtcgcaac tgtggtggat taaatccgtc accgggcaaa gcgaaaccat
    55141 tgtgctgtgg cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaaccgtaca
    55201 gcttactgcg ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt
    55261 caaagcgcca ggattgctgc atattgtcgg gcgcaacggc ggacgtaagc tgaagaaaat
    55321 ctggcaagag ttaggcgtgc cgccgtggct acgtgacacc acgccactgc tgttttatgg
    55381 agaaacgctg attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgaaggtga
    55441 gaatggcgta agttttgtct ggcagaaaac gcttagttaa gtgaaagccg gataagacgc
    55501 atcaaacgtc gcatccggcg aaagtcaatc aggattcgct taccaccacc gtaccgattt
    55561 cagggtggct aaagctggta attttatcca gacgcagctc gcgggtttcg ccagcggcct
    55621 cgacgaccag atactccaca tttttgcggg agactaaatc actggctttt gcctgcaatt
    55681 tttcgccatc tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat
    55741 tatcgtaatc atcacaatta attggttgat acgtatcatt cattgacata atcgctcacc
    55801 agtaagtttg ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg
    55861 gcagccattt cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag
    55921 tctccgctgg cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc
    55981 cctcctgtca gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc
    56041 tgtttcgcaa ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt
    56101 attccgttac aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa
    56161 tttataaagc gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc
    56221 tgacgctggc gcgccaccct tcagaaaccc aggagcgtat gatgctgcgc ttgctggcgt
    56281 ggctgaaata tgctgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc
    56341 cggaagcgtg gctgcgtaac gatcacctgg gcattgattt gtggattgag ctggggctgc
    56401 cggatgagcg acgaattaag aaagcctgca cccaggccgc agaagtggcg ctgtttacct
    56461 ataatagtcg ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gtgcagtttg
    56521 ccaatctttc cgtctggtat ctggacgatg aacaactggc gaaagtaagc gcctttgccg
    56581 atcgtaccat gacgctgcag gcaacgattc aggatggtgt tatctggtta tcggatgata
    56641 agaataatct ggaagttaac ttaaccgcct ggcaacaacc ttcatgattg tgatttcccg
    56701 acatgttgct attcccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgctgg
    56761 cgggcagcat gttaataaga cctcaacggc tattcatctg cgttttgaca ttcgggcgtc
    56821 cagcctgcca gagtattaca aagagcgtct gctcgccgcc agccatcatt tgatcagcag
    56881 tgatggcgtg attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc
    56941 cgctctggcc cggctggtgg cagtgattaa agatttaaca aaagaacaaa aagcccgacg
    57001 acccacgcgg cccacccgtg catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc
    57061 aagcgtgaag gcgatgcgcg gcaaagtacg cagcggtcgg gaataaaaag aaggaatgga
    57121 tggtgaaaaa agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat
    57181 gtaataatcg ggccgaagtc gatacgcttt ctccggcgca ggctgccgaa ctgaaaccga
    57241 tgccgcaaag ttggcgcggc gtgctaccgt gtgccgattg cgaaggaatc gaaatctctc
    57301 tgttcctcga aaaagacgga acatgggtga tgaatgagcg ttatctcggt gctcgtgaag
    57361 aaccttcctc cttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa
    57421 ccgacagcaa aggtgaaaag tcatattatc gggcgaaagg cgatgcgctg gagatgctcg
    57481 atcgtgaagg caatccaatt gaatcgcagt tcaactatac gctggaaccg gcacaatcca
    57541 gtttacctat gacgccgatg accctgcgag gcatgtattt ttatatggct gatgcggcga
    57601 ccttcactga ttgcgcgacc ggaaaacgtt tcatggtagc gaataacgca gagctggagc
    57661 gtagctacct ggctgcgcgc ggtcacagtg aaaaaccggt gttactgtca gtagaaggtc
    57721 actttacgct tgaggctaat ccggatactg gtgcgccaac caaagtatta gcgcccgata
    57781 cggcgggtaa attttacccg aataaggatt gcagtagttt ggggcagtaa acctgcttaa
    57841 ggcagaaaca aacgcaaaat tgcctgatgc gctgcgctta tcaggcctac agcatataat
    57901 gcaacatttt gaattagcac gattttgtag gccggataag gcgtttacgc cgcatccggc
    57961 attttgtgca cgaggctggc tttatcactg agtcagccca acaaagcgac ccgccttgat
    58021 ataaatccca ggcttcaggt gcccaacata ctgtaactgc gtttctggct taaacgcaga
    58081 gacatcacca atacgcatat gcatcagatc ggcagggcta atccagcccg attgcgccag
    58141 tgtaagtgga tgcccggatt tggcaaatgc atcagtgaca aattccgaac aaaaccacga
    58201 ctttttgtct ccttcgcaca cactgcttaa ctgcgctttc gccaggtcgc tgacgcactg
    58261 ttggcgaaaa tcctcggaga acggattcag tgagcacatc tggcgagtca ccataaaggg
    58321 aataaattcg acaatgccgc gatagttata accgctatct ttgattttat tggcaaacgc
    58381 ggtgatatct gtggcttgtt gcggggtaag atccgggact cgtaagacga aaagcttatc
    58441 actatgcttc atcgcttttt taagggaaac aatctggacg ccagcgcctg tcgcttctgc
    58501 aacgttatta tcacccagaa agattgcaac gtgactcaca gaggaagtgc tgaagacgcg
    58561 gattccaaat gaggttaccc caaggcttga ggagaacagc aaatcgccgg gtttgagatc
    58621 aggtgccgtt atttctttta ttgattgttc ggtgaaagag ctttgatgct ggaatttgac
    58681 agcccatgtt tttgcctcgg catctactgc tgtggctgat gggtctggct ggctgatatc
    58741 aacggtacaa gccgaaagta aaagaaagct ggggagaagc aggcggcagt acgcctttgg
    58801 tttatccatt ttatacaatc catgtaaaaa agggccctga aattcaggac cctttctggc
    58861 atcagccttt aatctgtttc accagatatt cgacgatgtc accagtctta attaactgtt
    58921 tctcgccgtt gcgacgatat ttatattcga tatcgtcgtt gtcgaggtta cggtcgccca
    58981 gcacaatagt gtgcggaata ccgatcagtt ccatatcagc aaacatcacg cccggacgct
    59041 ctttgcggtc atccagcagc acttcgatac cttgtgcacg cagttcgctg tacagtttct
    59101 cagcaagctc ttgtacgcgg aaggatttgt gcatgttcat cggcagaatc gccacctgga
    59161 acggcgcgat agcgtcaggc catacgatgc cgcgttcgtc gtagttctgc tcaatcgccg
    59221 cagctaccac acgcgttacc ccgataccgt agcaacccat cgtcaggatt tggttacggc
    59281 catcttcacc ctgtacggag gctttcagtg cttcggagta cttggtaccc agctggaaga
    59341 tgtgaccaac ttcgatacca cgtttgatca gcagcgtacc ctggccatcc gggcttggat
    59401 cgccagccac cacgttacgg atatctgcaa cttccggggt agcgacatcg cgatcccagt
    59461 tgataccgaa gtagtgttta ccatcgatgt tagcaccagc agcgaaatca ctcatcgccg
    59521 caacggtacg gtcaatcacc accggaatcg gcatgtttac cggacccagt gaacccggac
    59581 cggctttaac cacagcacga atttcttctt cggtcgcgaa agtcagcggg cttgcaacct
    59641 gcggcagttt ttctgcttta acttcgttca gctcgtgatc accgcgcacc agcagcgcaa
    59701 ccagcgggaa gctactgcct tcaaccgctt taaccagcag agtcttaacc gttttctcaa
    59761 tcggcagatt gaactgttca accagttccg cgatggtttt cgcgttcggc gtatcaacca
    59821 gcgtcatttc ctgggtagca gcagcgcgcg gttctttcgg cgcgatagct tctgccagtt
    59881 caatgttcgc tgcatagtca gaggtgtcgg agaagaccac atcgtcttca ccgctctgcg
    59941 ccagcacctg gaattcgtga gaggcgctgc cgccgataga accggtgtcg gcctgtacgg
    60001 cgcggaaatc cagccccatg cggctgaaga ttttgctgta ggccgcatac attgcatcgt
    60061 aggtttcctg cagggattcc tgagaagtat ggaaagagta agcatctttc atcaggaatt
    60121 cgcgggaacg catgacgccg aaacgcggac gcacttcgtc gcggaacttg gtctggatct
    60181 gatagaagtt cagcggcagc tgtttgtaag agctaagctc gttacgaatc aggtcagtga
    60241 taacttcttc atgagttggg ccgagtacga acggacgctc gccacggtca acaaaacgca
    60301 gcagttccgg accgtactgt tcccaacgac cactctcttg ccacaaatcg gctggctgaa
    60361 ccaccggcat cgacacctcg atcgcaccgg cgttgttcat ctcttcacgc acgatgtttt
    60421 cgactttttt cagaacgcgc acgccggtcg gcagccaggt atataacccg gaggccagct
    60481 tgcggatcat cccggcgcgc agcatcagct gatggctgat cacctcggcg tcggcaggtg
    60541 tctccttgag agtggagagc aggtattggc tagtacgcat gttgttacgg ttccagttgg
    60601 aaggtagaac aggctcaagg cgagcctggg acaaaaaaag tgatttagtt taccagtgca
    60661 aaagaaatgt caaaagagaa gggcgtgaat ttaacgcggt tccagcgcaa agacttcaaa
    60721 acctgcgtcg gtgacgcgcc agcgaacgtt aaaatcatgt agccagacgg cataggtttt
    60781 gcccgtttcc tcacctttac gataggccgg gcgcgggtcc tgcgccagta cttcgcggat
    60841 aaacagcgtt aactgcggat aacgcttctc cagcgtcaaa agctgctttt cgacctctgc
    60901 ggtaaaactc accgccatct ctgcagctgg cgcactttgc gcatagctgg cactggcatc
    60961 gggaagcgat tcggcaaagg ggagatacgg tttgatatcc actaccggcg taccatcgac
    61021 cagatccaga ctgccgagct tcagaatcac gctgccttta tggcaaacaa cctctttcag
    61081 ctctaccagc gacatgccaa ttgggttagg gcggaaagta gagcgtgttg cgaaaacccc
    61141 cattctggcg ttaccgccga ggcgcggtgg acgcacagtc ggacgccagc cgccttccat
    61201 cgtttgatga aagacgaaaa ggatccataa atggctgaat gcttccaggc cgcgaacggc
    61261 gtcggcctgg ttgtaggaag ctatgagatg cagttcgccg ttggcgcttt ttaccagacc
    61321 tggctggcgc ggaacggcga atttttcttt atagggcgag cgaataacgc ctatttgctc
    61381 gaactggaaa ctgctcattt cgccgtaatg ttaagcgcag agccgataca tacagcctga
    61441 cgatagcagc ccggcgtacc gctggtgact tcgcagctat gcagtaatac agcattggct
    61501 ttcattttag aggcattgat ttgcatctgc ttacgtgcgg ttggaatgct cggcggagag
    61561 tcctgattag aggcctggca agagtcgcca ctgacttcac cgagatcgcg gaacggtttg
    61621 ccgactaatt cttctgcatt ggtataaatt cggaccggcg tggcgcgcgg cgctttcggt
    61681 tttgcaggct ccgctttcgg ctggggtgca gtgctttgaa cgggttcgac aggggatctg
    61741 cttaacatgg aacagccgct tagcatgagt gctactaaac agatcggtaa agcacgcata
    61801 gtatttcctc aatgtatgat caaaacgtca atattgaatc aggagcttgt aaaaatgaca
    61861 agacgggcaa gcgcccgtcc tgaatgatat tacaaattgt ggaaacagcc taaaaattac
    61921 cagcctttaa cagcgccgcc gttaaacact ttgtttgctg cttcgtaaac ttcatcagac
    61981 tgataagcct ggacgaattt cttcacgttc tcggcgtctt tgttatcttc acgcgttacg
    62041 atcaggttta cgtatgggga gtctttatct tcgacaaaga taccgtcttt cgccggagtc
    62101 aggccaatct ggctggcata ggtggtattg ataactgcca gagcgatttg cgcgtcgtcc
    62161 agagaacgcg gcagttgcgg tgcttccagt tcaacaattt tcagattttt tgggttctca
    62221 acaacatcaa gaacggtcgg cagcaggcca acgccatctt tcagtttgat caagcccact
    62281 ttttgcagca gcagcagtga acgaccaaga ttagttggat cgtttggcac ggcaacctgc
    62341 gaaccatcct gcagttcatc cagtgatttg attttcttgg agtaaccagc aatcggataa
    62401 acaaaagtgt tgcctactgc gaccagtttg tagccacgat ctttcagttg ctgatcaagg
    62461 tacggtttat gctggaaggc gttggcgtcg atatcgcctt tgctcaatgc ttcgtttggc
    62521 agaacatagt cgttgaaggt taccagctca acgtccaggc catatttgtc tttcgcaact
    62581 ttctgcgcga cttctgctac ctgctgttcg gcaccaacaa tcacgccgac tttaatgtgg
    62641 tttggatctt tttcatcctg accgcagcct accagtgcca gtgatccaat cagggctccc
    62701 actgccgcaa aggttttgaa tttgaacgcc ataccttatt ccttcttcaa ttatttatgt
    62761 tgtgttgaac gttacttgcg agtgacagcc cggacgatgc ggtcgcctgc aaactgaatt
    62821 aaataaacca gaatgaccag caataccagt accgtattca tcaccgtcgc gttgtagccg
    62881 atgtagccat actgatagcc aatctgacct aaaccaccgg caccgactgc accacccatc
    62941 gcggaataac cgaccagggt aatcagggtg atagttgccg cattcaccag acccggcaac
    63001 gcttccggta acagcacttt acagacgatc tgcattggcg tggcacccat tgcgcgggaa
    63061 gcttcaatta acccggttgg gatctccagc agagcgttct cgaccatacg ggcaataaac
    63121 ggtgctgcac caacggttaa cggaacaatt gctgcctgca aaccaatcga tgtaccgaca
    63181 ataacgcggg taaacggaat catccatacc agcaagataa tgaacgggat ggaacggaaa
    63241 atgttcacaa tcgcagaaac ggtacgatac agcttcgcgt tagcaataat ttgccccgga
    63301 cgcgtgacat aaagcagaac gccaaccggc agaccaatca caaacccaaa aaatccggat
    63361 acgaaggtca ttgccagcgt ttcccatacg ccacgaacca gcagccacat catcggctca
    63421 gacataaccc agtacctcta cttttacatg gtgttcctgc agccaggcaa tggcggcttg
    63481 cgtatcttgt tgtgtgccgt gcatttcagt cagcatgatg ccgaatttaa cgccaccggc
    63541 gtagtccatc tgcgcgctaa taatgttgtt gttgacgttg aaacgacgcg cggtttcaga
    63601 aagcagtggg gcatcgaccg attgaccggt aaactccaga cgcagcatcg gcacgcagtc
    63661 agtaaatggc tccgtttgca gacgttcctg gtaatcttcc gggatatcca gatgcagggt
    63721 cgactgaata aacttctgcg ccagcggcgt tttcggatgc gagaacactt cacttaccgt
    63781 gtcctgctcg atcagttcgc cattgctgat gaccgccacg caatcacaaa tacgcttcac
    63841 aacgtccatt tcgtgggtga tcaacagaat cgtcaaaccc agacggcggt taatgtcttt
    63901 cagcagttcg agaatagaac gtgtcgttgc cgggtccagc gcgctggtgg cttcatcaca
    63961 cagcaatact ttgggattgc tggctaatgc acgggcaatc gccacacgtt gtttctgccc
    64021 accggaaaga ttcgacgggt agctatcatg cttatcgcca agaccaacca atgacagcaa
    64081 ttccgtcacg cgacgtttga tctcgtcttt cggtgtgttg tccagctcca gcggcagagc
    64141 cacgttgcca aaaacagtac gcgaagagag caggttaaaa tgctggaaaa tcataccaat
    64201 ctggcggcga gctttggtca actcggattc tgacagcgtg gtcaattcct ggccatcgac
    64261 cagcacgcta ccctcggttg ggcgctccag caagtttaca caacgtataa gcgtactctt
    64321 acccgcgcct gaggcaccga taacgccata aatttgtcca gctggcacat gcaggctgac
    64381 gttgttcaac gcctggatgg tgcgggtgcc ctggtggaac actttggtga tattcgaaag
    64441 ttttatcatt gattatttat tatcgtcatt aagttagtcg tagcatctcg aatgcctgaa
    64501 acgggcaacg ccgtcaatga aatggatgtt aaggcatcca gacgtctaaa tcaatcaggt
    64561 ttatgcgaag agcactttct tgcaggtcga aacatgcgat actagcgtcc catgccttat
    64621 taaggagcta taaaaggtgg cgaagagcgt acccgcaatt tttcttgacc gtgatggcac
    64681 cattaatgtc gatcacggct atgtccatga gatcgacaac tttgaattta tcgacggtgt
    64741 tattgacgcc atgcgcgagc taaaaaaaat gggctttgcg ctggtggtag taaccaacca
    64801 gtctggcatt gctcgcggta aatttaccga agcacagttt gaaacgctga ccgagtggat
    64861 ggactggtcg ctggcggacc gagatgtcga tctggatggt atctattatt gcccgcatca
    64921 tccgcagggg agtgttgaag agtttcgcca ggtctgcgat tgccgcaaac cacatccggg
    64981 gatgcttttg tcagcacgcg attatttgca tattgatatg gccgcttctt atatggtggg
    65041 cgataaatta gaagatatgc aggcagcggt tgcggcgaac gtgggaacaa aagtgctggt
    65101 gcgtacgggt aaacctatta cacctgaagc agaaaacgcg gcagattggg tgttaaatag
    65161 cctggcagac ctgccgcaag cgataaaaaa gcagcaaaaa ccggcgcaat gattaaaaga
    65221 tgaacggttg aaataaaaat gcatttttcc gcttgtcttc ctgagccgac tccctataat
    65281 gcgcctccat cgacacggcg gatgtgaatc acttcacaca aacagccggt tcggttgaag
    65341 agaaaaaatc ctgaaattca gggttgactc tgaaatagga aagtgtgata tacgccacct
    65401 cgcgacagtg agctgaaagc tgcgtcgcaa ctgctcttta acaatttatc agacaatctg
    65461 tgtgggcact cgaagatacg gattcttaac gtcgcaagac gataaatgaa taccaagtct
    65521 caagagtgaa cacgtaattc attacgaagt ttaattcttt gagcatcaaa cttttaaatt
    65581 gaagagtttg atcatggctc agattgaacg ctggcggcag gcctaacaca tgcaagtcga
    65641 acggtaacag gaagcgcttg ctgcttcgct gacgagtggc ggacgggtga gtaatgtctg
    65701 ggaaactgcc tgatggaggg ggataactac tggaaacggt agctaatacc gcataacgtc
    65761 gcaagaccaa agagggggac cttcgggcct cttgccatcg gatgtgccca gatgggatta
    65821 gcttgttggt ggggtaacgg ctcacc
//
LOCUS       DAJTUP010000009        62497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTUP010000009 DAJTUP010000000
VERSION     DAJTUP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.009"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(24..1082)
                     /gene="modC"
                     /locus_tag="OO468_000586"
     CDS             complement(24..1082)
                     /gene="modC"
                     /locus_tag="OO468_000586"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR6257825.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1085..1774)
                     /gene="modB"
                     /locus_tag="OO468_000587"
     CDS             complement(1085..1774)
                     /gene="modB"
                     /locus_tag="OO468_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR6257826.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(1774..2547)
                     /gene="modA"
                     /locus_tag="OO468_000588"
     CDS             complement(1774..2547)
                     /gene="modA"
                     /locus_tag="OO468_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6257827.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(2714..2863)
                     /gene="acrZ"
                     /locus_tag="OO468_000589"
     CDS             complement(2714..2863)
                     /gene="acrZ"
                     /locus_tag="OO468_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR6257828.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            2992..3780
                     /gene="modE"
                     /locus_tag="OO468_000590"
     CDS             2992..3780
                     /gene="modE"
                     /locus_tag="OO468_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR6257829.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            3848..5320
                     /gene="modF"
                     /locus_tag="OO468_000591"
     CDS             3848..5320
                     /gene="modF"
                     /locus_tag="OO468_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR6257830.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            5581..6597
                     /gene="galE"
                     /locus_tag="OO468_000592"
     CDS             5581..6597
                     /gene="galE"
                     /locus_tag="OO468_000592"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR6257831.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            6607..7653
                     /gene="galT"
                     /locus_tag="OO468_000593"
     CDS             6607..7653
                     /gene="galT"
                     /locus_tag="OO468_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR6257832.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            7657..8805
                     /gene="galK"
                     /locus_tag="OO468_000594"
     CDS             7657..8805
                     /gene="galK"
                     /locus_tag="OO468_000594"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR6257833.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            8799..9839
                     /gene="galM"
                     /locus_tag="OO468_000595"
     CDS             8799..9839
                     /gene="galM"
                     /locus_tag="OO468_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR6257834.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10042..10794
                     /gene="gpmA"
                     /locus_tag="OO468_000596"
     CDS             10042..10794
                     /gene="gpmA"
                     /locus_tag="OO468_000596"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR6257835.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(10961..12013)
                     /gene="aroG"
                     /locus_tag="OO468_000597"
     CDS             complement(10961..12013)
                     /gene="aroG"
                     /locus_tag="OO468_000597"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR6257836.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            12329..12709
                     /locus_tag="OO468_000598"
     CDS             12329..12709
                     /locus_tag="OO468_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR6257837.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            12823..13764
                     /gene="zitB"
                     /locus_tag="OO468_000599"
     CDS             12823..13764
                     /gene="zitB"
                     /locus_tag="OO468_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR6257838.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(13761..14480)
                     /gene="pnuC"
                     /locus_tag="OO468_000600"
     CDS             complement(13761..14480)
                     /gene="pnuC"
                     /locus_tag="OO468_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR6257839.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(14518..15561)
                     /gene="nadA"
                     /locus_tag="OO468_000601"
     CDS             complement(14518..15561)
                     /gene="nadA"
                     /locus_tag="OO468_000601"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR6257840.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(15810..15885)
                     /locus_tag="OO468_000602"
     tRNA            complement(15810..15885)
                     /locus_tag="OO468_000602"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15850..15852),aa:Lys,seq:ttt)
     gene            complement(15889..15964)
                     /locus_tag="OO468_000603"
     tRNA            complement(15889..15964)
                     /locus_tag="OO468_000603"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15929..15931),aa:Val,seq:tac)
     gene            complement(16016..16091)
                     /locus_tag="OO468_000604"
     tRNA            complement(16016..16091)
                     /locus_tag="OO468_000604"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16056..16058),aa:Lys,seq:ttt)
     gene            complement(16094..16169)
                     /locus_tag="OO468_000605"
     tRNA            complement(16094..16169)
                     /locus_tag="OO468_000605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16134..16136),aa:Val,seq:tac)
     gene            complement(16305..16380)
                     /locus_tag="OO468_000606"
     tRNA            complement(16305..16380)
                     /locus_tag="OO468_000606"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16345..16347),aa:Lys,seq:ttt)
     gene            complement(16545..17336)
                     /gene="cpoB"
                     /locus_tag="OO468_000607"
     CDS             complement(16545..17336)
                     /gene="cpoB"
                     /locus_tag="OO468_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR6257841.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(17346..17867)
                     /gene="pal"
                     /locus_tag="OO468_000608"
     CDS             complement(17346..17867)
                     /gene="pal"
                     /locus_tag="OO468_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR6257842.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(17902..19194)
                     /gene="tolB"
                     /locus_tag="OO468_000609"
     CDS             complement(17902..19194)
                     /gene="tolB"
                     /locus_tag="OO468_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR6257843.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(19327..20568)
                     /gene="tolA"
                     /locus_tag="OO468_000610"
     CDS             complement(19327..20568)
                     /gene="tolA"
                     /locus_tag="OO468_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR6257844.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(20633..21061)
                     /gene="tolR"
                     /locus_tag="OO468_000611"
     CDS             complement(20633..21061)
                     /gene="tolR"
                     /locus_tag="OO468_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR6257845.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(21065..21757)
                     /gene="tolQ"
                     /locus_tag="OO468_000612"
     CDS             complement(21065..21757)
                     /gene="tolQ"
                     /locus_tag="OO468_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR6257846.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(21754..22158)
                     /gene="ybgC"
                     /locus_tag="OO468_000613"
     CDS             complement(21754..22158)
                     /gene="ybgC"
                     /locus_tag="OO468_000613"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR6257847.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(22308..22601)
                     /gene="ybgE"
                     /locus_tag="OO468_000614"
     CDS             complement(22308..22601)
                     /gene="ybgE"
                     /locus_tag="OO468_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR6257848.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(22601..22714)
                     /gene="cydX"
                     /locus_tag="OO468_000615"
     CDS             complement(22601..22714)
                     /gene="cydX"
                     /locus_tag="OO468_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR6257849.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(22729..23868)
                     /gene="cydB"
                     /locus_tag="OO468_000616"
     CDS             complement(22729..23868)
                     /gene="cydB"
                     /locus_tag="OO468_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6257850.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(23884..25452)
                     /gene="cydA"
                     /locus_tag="OO468_000617"
     CDS             complement(23884..25452)
                     /gene="cydA"
                     /locus_tag="OO468_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR6257851.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(26301..28934)
                     /gene="mngB"
                     /locus_tag="OO468_000618"
     CDS             complement(26301..28934)
                     /gene="mngB"
                     /locus_tag="OO468_000618"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR6257852.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(28952..30868)
                     /gene="mngA"
                     /locus_tag="OO468_000619"
     CDS             complement(28952..30868)
                     /gene="mngA"
                     /locus_tag="OO468_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR6257853.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            31038..31760
                     /gene="mngR"
                     /locus_tag="OO468_000620"
     CDS             31038..31760
                     /gene="mngR"
                     /locus_tag="OO468_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR6257854.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(31863..32732)
                     /gene="sucD"
                     /locus_tag="OO468_000621"
     CDS             complement(31863..32732)
                     /gene="sucD"
                     /locus_tag="OO468_000621"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR6257855.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(32732..33898)
                     /gene="sucC"
                     /locus_tag="OO468_000622"
     CDS             complement(32732..33898)
                     /gene="sucC"
                     /locus_tag="OO468_000622"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR6257856.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(33992..35209)
                     /gene="odhB"
                     /locus_tag="OO468_000623"
     CDS             complement(33992..35209)
                     /gene="odhB"
                     /locus_tag="OO468_000623"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR6257857.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(35224..38025)
                     /gene="sucA"
                     /locus_tag="OO468_000624"
     CDS             complement(35224..38025)
                     /gene="sucA"
                     /locus_tag="OO468_000624"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR6257858.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(38326..39042)
                     /gene="sdhB"
                     /locus_tag="OO468_000625"
     CDS             complement(38326..39042)
                     /gene="sdhB"
                     /locus_tag="OO468_000625"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR6257859.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(39058..40824)
                     /gene="sdhA"
                     /locus_tag="OO468_000626"
     CDS             complement(39058..40824)
                     /gene="sdhA"
                     /locus_tag="OO468_000626"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR6257860.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(40824..41171)
                     /gene="sdhD"
                     /locus_tag="OO468_000627"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(40824..41171)
                     /gene="sdhD"
                     /locus_tag="OO468_000627"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR6257861.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(41165..41569)
                     /gene="sdhC"
                     /locus_tag="OO468_000628"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(41165..41569)
                     /gene="sdhC"
                     /locus_tag="OO468_000628"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6257862.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            41950..42057
                     /locus_tag="OO468_000629"
     CDS             41950..42057
                     /locus_tag="OO468_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257863.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            42263..43546
                     /gene="gltA"
                     /locus_tag="OO468_000630"
     CDS             42263..43546
                     /gene="gltA"
                     /locus_tag="OO468_000630"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR6257864.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            43937..44503
                     /locus_tag="OO468_000631"
     CDS             43937..44503
                     /locus_tag="OO468_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6257865.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            44554..47013
                     /locus_tag="OO468_000632"
     CDS             44554..47013
                     /locus_tag="OO468_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6257866.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            47028..47759
                     /locus_tag="OO468_000633"
     CDS             47028..47759
                     /locus_tag="OO468_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6257867.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            47756..48832
                     /locus_tag="OO468_000634"
                     /pseudo
     CDS             47756..48832
                     /locus_tag="OO468_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            48984..50030
                     /locus_tag="OO468_000635"
     CDS             48984..50030
                     /locus_tag="OO468_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR6257868.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(50027..50818)
                     /gene="nei"
                     /locus_tag="OO468_000636"
     CDS             complement(50027..50818)
                     /gene="nei"
                     /locus_tag="OO468_000636"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR6257869.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(50854..51588)
                     /gene="pxpA"
                     /locus_tag="OO468_000637"
     CDS             complement(50854..51588)
                     /gene="pxpA"
                     /locus_tag="OO468_000637"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR6257870.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(51578..52510)
                     /gene="pxpC"
                     /locus_tag="OO468_000638"
                     /pseudo
     CDS             complement(51578..52510)
                     /gene="pxpC"
                     /locus_tag="OO468_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(52504..53160)
                     /gene="pxpB"
                     /locus_tag="OO468_000639"
     CDS             complement(52504..53160)
                     /gene="pxpB"
                     /locus_tag="OO468_000639"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR6257871.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(53183..53926)
                     /gene="ybgI"
                     /locus_tag="OO468_000640"
     CDS             complement(53183..53926)
                     /gene="ybgI"
                     /locus_tag="OO468_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR6257872.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            54197..55678
                     /gene="dtpD"
                     /locus_tag="OO468_000641"
     CDS             54197..55678
                     /gene="dtpD"
                     /locus_tag="OO468_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR6257873.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(55828..57246)
                     /gene="phrB"
                     /locus_tag="OO468_000642"
     CDS             complement(55828..57246)
                     /gene="phrB"
                     /locus_tag="OO468_000642"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR6257874.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(57243..57752)
                     /locus_tag="OO468_000643"
     CDS             complement(57243..57752)
                     /locus_tag="OO468_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR6257875.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            57881..58168
                     /locus_tag="OO468_000644"
     CDS             57881..58168
                     /locus_tag="OO468_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR6257876.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(58223..58603)
                     /locus_tag="OO468_000645"
     CDS             complement(58223..58603)
                     /locus_tag="OO468_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257877.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(58606..61992)
                     /locus_tag="OO468_000646"
     CDS             complement(58606..61992)
                     /locus_tag="OO468_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6257878.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDAPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(62011..62214)
                     /locus_tag="OO468_000647"
                     /pseudo
     CDS             complement(62011..62214)
                     /locus_tag="OO468_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15193 a        16894 c        15216 g        15194 t
ORIGIN      
        1 tcatttatag ccacctgatt taatcaggcg gttatcgaca cacttttaat ttgcgcgtac
       61 agccacaggc caggtttgat cgccagttca tccctggccc acgggctgat acgcgcccac
      121 agcgttttac cgccgacttc cagctccact tccacctggc cgttgtcgtc ataactatta
      181 acaacttttg cccgcaatac gttacgaatg ctggtttgct gcggcggttg taaaaccaga
      241 gaaacatcgg aagcctgaat gcggatacgt agcgcagctt gcagcggttc gcccagctta
      301 ttgacccaca aatgctgatc gcccagcgcc agcgcggtca tcgcgtaatg cggatgatgc
      361 tcaagcaccg tcactttcag aatgctactt tgttgctctt tcggcagcca cggattcatc
      421 acgctactgc cccacacttc ctccagcgcg ccaaaggctt tcacctgacc gttttccagt
      481 accatcactc tgtctgccag atggaggatc tcatccagcg aatggctgac atacaacatc
      541 ggaatgttga tttcccgtgt cagccgttgc agataaggca acagttcgcg tttacgcgga
      601 atatccagtg acgccagcgg ttcatccagc aacagcaatt ccggtgctgt cagcaaagcc
      661 cgaccaatcg ccacgcgctg tttttcgcct ccggacaggc tgcctggtaa acggtcaagc
      721 aacggttcaa tgcctaaaag cgccaccagc ttatcgaact gatcgaccat acttttcgac
      781 atgccgtagc gcagattgcc acgcactttg taatgcggga acaaccgcgc atcctgaaaa
      841 acatagccaa cacgacgctt ttccggcgtc aggcagatac ctttttcggc atcatttagt
      901 acccgcccat tgaggacaat ccgccctttt tgcgggcgcg tcagtccact gatggcgtta
      961 atcagcgaag tttttccggc accggagacg ccaaagatag cagtgatgcc attggcgggc
     1021 agcgtttcat taatggtcag gcaatggttg cccaacgtct gggaaaaatt cagctccagc
     1081 atgattagcg ccccgcccgt tcacggctga ttctggccag ccattctgaa atcaacaggg
     1141 agatcatcgc cagcgcaata gaaataatgc acagtctcgc cgcgccactt tccccgccgg
     1201 gggtctggat cagggtatac atggcagaag gaatggttcg cgtttcgcta ggaatgttcg
     1261 acacaaaggt gatggttgca ccaaactcac cgagagaacg agcaaaagcc agtaccgtac
     1321 caacaataat tcccggtaag gtcagcggta acgtgatagt aaagaaaacg cgccagcgcc
     1381 cggcacctag tgttcttgcg gcctgttcca gtttgacatc aaccccttcc agcgccagac
     1441 gaattgcccg caccatcagc ggaaacgaca tgacggcggc agcgagaacc gcgccgcgcc
     1501 agctaaaggc gaaggtaata ccaaaccagt catacagacg ttcaccgata aatccgcgcc
     1561 gtcccatcga aactaataat aagtaaccga cgaccacggg cggtaacacc agcggtagat
     1621 gcagtacgct gtcgagcaga gctttgcccg gaaacgtgca acgcaccagt aaccaggcaa
     1681 aaaagatccc aaacggcagg ctaaacagca cagccaggga agaaactttc aggcttaata
     1741 aaactgcctg ccattctgga tcggtcagta tcattacttg gttgtaaatc cgtaacgttt
     1801 aaagatttcc gctgcctgcg gtcccttcag ataatcataa aatgctttca ctgtcgcatt
     1861 gttatgccct tccacaacag caaccggata ttccactttt ttatgtgaat cttccgggaa
     1921 ggtggcaacc acttttaccc ctttgctggc aactgcgtca gaaccgtaga caatgcccag
     1981 aggcgcttcg ttacgttcga ccagcgccag cgccccacga acatcttccg ctggggccag
     2041 tttcggagag agcgtatccc atgcgcccag tttttgcagt gcttcttttg cataaatgcc
     2101 agcgggaaca tgttccggat cgccaaccgc caggcgaccg ccattcagca gcgaagtcca
     2161 gttagttttg ctgtcgatgg tgaaatcttt ctgctcgctg gctttcggtg ctacaacgac
     2221 caggctattg ccgagcagtg tctgacgcgt agcagtatcg atcgcttttt tatcaaccgc
     2281 ataatccatc catttctgat cggcagaaat aaacagatcc gcaggggcac ccgcttcaat
     2341 ctgacgggcg agagtagatg acgaagcgaa agaagaaacc acatccacgc ctttctcttt
     2401 tttatactgc gtagcaatgt cctgcattgc gttagtcagt gatgcggcgg cgaacaccgt
     2461 gattttccct tcatctgcca gtgcattgcc agcaactgcg aaagagagtg ccgccccggc
     2521 aaacaagttc aaccatttac gagccattgg taacccctta atgtaacgtt atgtaggcga
     2581 caatataacg actaactcgg aattttccca gcagttattg ctaacctttg tgaggtagat
     2641 aagaaaaaat atcggcagga aaagcaggaa gttgagaaaa agaaaatgcc cgactaagcg
     2701 ggcactcagg gaatcaatga ttttgtccgg gctggtcttt tttaccaaca ccagaaaaga
     2761 tgttgaatac ttcaccaaga ccgtaaatca gacccaggat gatggccatc acgacaggta
     2821 ccatgattac ggcgaatacc agacttttta ataactctaa catggtcaac tccagatata
     2881 gtcatgagac tattctaacc gctaagcaca gaaaagcact ccccttttgt gcggtcagct
     2941 ttgcgtggcg ttcgttttcc gtcacaataa gacttttgcc aggacattgt tatgcaggcc
     3001 gaaatccttc tcacccttaa gctccaacaa aaattattcg ccgacccgcg ccgcatttcg
     3061 ctactaaaac acattgcgct ttccggttcc attagccagg gagcgaaaga tgccggtatc
     3121 agctataaaa gcgcctggga tgccattaac gagatgaatc agttaagtga gcatattctg
     3181 gtcgagcgcg caacaggcgg taaaggtggc ggaggcgcag tactgacccg ctatggtcag
     3241 cgactgattc agctctatga cttactggcg caaatccagc aaaaagcctt tgatgtgtta
     3301 agtgacgatg acgccctgcc gctgaacagc ctgctggccg cgatctcacg tttttcactg
     3361 caaaccagcg cccgtaacca gtggttcggt accatcaccg cccgcgatca tgatgacgtt
     3421 caacagcatg ttgatgtctt actggctgac ggaaaaacac gcctgaaagt cgcaattacc
     3481 gcacaaagcg gcgcacgtct ggggctggat gaaggcaaag aagtgttgat attgctaaaa
     3541 gcgccgtggg taggcattac tcaggacgaa gcggtcgcac aaaacgccga caaccaatta
     3601 ccgggtatta ttagtcatat tgagcgcggc gcagagcagt gcgaagtatt aatggcgcta
     3661 cccgacgggc aaacactgtg cgccacagtg ccggtaaatg aagcgacttc tcttcagcaa
     3721 gggcagaatg tcacggccta ctttaatgcc gacagcgtga ttatcgccac gctgtgctaa
     3781 gcgtgttgac aatttgttat gaaacacgta tccctgtcag taatcgctgc acaaaatggg
     3841 atataaaatg tcatcgttgc aaattttgca aggcacgttt cgtcttagcg acacaaaaac
     3901 gctgcaattg cctcagctaa cgttaaacgc gggtgatagt tgggcgtttg tcggttcgaa
     3961 tggaagtgga aagtcggcgc tggcccgcgc gctggcgggg gaacttccgc ttttgaaagg
     4021 tgaacggcaa agccagtttt cccacatcac tcgtctctcc ttcgagcaat tgcaaaagct
     4081 cgtcagcgac gaatggcaac ggaataacac cgatatgctc ggccctagcg aagattacac
     4141 cggacgcact acggctgaga ttattcagga tgaagtaaag gatgcaccgc gttgcatgca
     4201 actggcgcag cagttcggta ttaccgccct cctcgaccga cgctttaaat acctttccac
     4261 tggcgagacg cgaaaaaccc tgctgtgtca ggcgctgatg tcggagcctg acttgttgat
     4321 tcttgatgag ccgttcgatg gcctggatgt tgcctcacgt cagcagctgg ctgagcgact
     4381 cgcctcgtta catcagtccg gtattacttt ggtactggtg ctcaatcgct tcgataagat
     4441 cccggaattt gtccagtttg ctggcgtgct ggcggattgt acgttagcgg aaactggcgc
     4501 taaagaggaa ctgctccagc aagcactcgt cgcgcaactt gcgcatagtg aacagcttga
     4561 aggtgtgcaa ctgccggagc cggatgaacc ttcagcacgt cacgccttac ccgccaacga
     4621 atcgcgcatt gtgctgaaca atggcgtggt ttcttataat gatcgcccca ttcttaataa
     4681 ccttagctgg caggtgaatc caggcgaaca ctggcaaatt gtcgggccaa atggcgcggg
     4741 aaaatcgacg ttattaagcc tgattactgg cgatcatccg caaggttaca gcaacgattt
     4801 gacgcttttc ggccgacgtc gcggcagcag cgaaaccatc tgggacatca aaaagcatat
     4861 cggttacgtc agcagtagtt tgcatctgga ttaccgggtc agcactaccg tgcgtaatgt
     4921 gattctttct ggctattttg attcgattgg catttatcag gtcgtttcgg atcgccagca
     4981 aaaactggtg cagcagtggc tggatattct cggcattgat aaacacacgg ctgacgctcc
     5041 gttccatagt ctttcctggg gacagcagcg tttggcgctg atcgtccgcg cactggtgaa
     5101 acatccgacg ttgcttattc tcgatgaacc actacagggg cttgatccgc tcaatcgcca
     5161 acttatccgc cgttttgttg atgtgctgat tagcgaaggt gaaacgcaat tgttgtttgt
     5221 ttcgcaccac gctgaagatg cgcctgcctg tattacccat cgccttgagt tcgtgccgga
     5281 cggtggactc tatcgctatg tgctgacaaa aatatattga gtcggtagtg ctgaccttgc
     5341 cggaggcggc ctaagcgcac tctccggcca acggttcgac gcatgcaggc atgaaaccgc
     5401 ttcttttttc agataaaaag cgcaatcatt cataaaccct ctgttttata atcacttaat
     5461 cgcgcataaa aaacggctaa attcttgtgt aaacgattcc actaatttat tccatgtcac
     5521 acttttcgca tctttgttat gctatggtta tttcatacca taagcctaat ggagcgaatt
     5581 atgagagttc tggttaccgg tggtagcggt tacattggaa gtcatacctg cgtgcaatta
     5641 ctgcaaaacg gtcatgatgt catcattctt gataacctct gtaacagtaa gcgcagcgta
     5701 ctgcctgtta tcgagcgttt aggcggcaaa catccaacgt ttgttgaagg cgatattcgt
     5761 aacgaagcgt tgatgaccga gatcctgcac gatcacgcta tcgacaccgt gatccacttc
     5821 gccgggctga aagccgtcgc cgaatcggta caaaaaccgc tggaatatta cgacaacaat
     5881 gtcaacggca ctctgcgcct gattagcgcc atgcgcgccg ctaacgtcaa aaactttatt
     5941 tttagctcct ccgccaccgt ttatggcgat cagccccaaa ttccatacgt tgaaagcttc
     6001 ccgaccggca caccgcaaag cccttatggc aaaagcaaat tgatggtgga acagatcctc
     6061 accgacctgc aaaaagccca gccggactgg agcattgccc tgctgcgcta cttcaacccg
     6121 gttggcgcgc atccgtcggg cgatatgggc gaagatccgc aaggcattcc gaataacctg
     6181 atgccataca tcgcccaagt tgctgtaggc cgtcgcgact cgctggcgat ttttggtaac
     6241 gattatccga ccgaagatgg tactggcgta cgcgattaca tccacgtaat ggatctggcg
     6301 gacggtcacg tcgtggcgat ggaaaaactg gcgaacaagc caggcgtaca catctacaac
     6361 ctcggtgctg gcgtaggcag cagcgtgctg gacgtggtta atgccttcag caaagcctgc
     6421 ggcaaaccgg ttaactatca ttttgcaccg cgtcgcgaag gcgaccttcc ggcctactgg
     6481 gcggacgcca gcaaagccga ccgtgaactg aactggcgcg taacgcgcac actcgatgaa
     6541 atggcgcagg acacctggca ctggcagtca cgccatccac agggatatcc cgattaagga
     6601 acgaccatga cgcaatttaa tcccgttgat catccacatc gccgctacaa cccgctcacc
     6661 gggcaatgga ttctggtttc accgcaccgc gctaagcgcc cctggcaggg ggcgcaggaa
     6721 acgccagcca aacaggtgtt acctgcgcac gatccagatt gcttcctctg cgcaggtaat
     6781 gtgcgggtga caggcgataa aaaccccgat tacaccggga cttacgtttt cactaatgac
     6841 tttgcggcct tgatgtctga cacgccagat gcgccagaaa gcaacgatcc tttgatgcgt
     6901 tgccagagcg cgcgcggtac cagccgggtg atctgctttt caccggatca cagtaaaacg
     6961 ttgccagaac tgagcgttgc ggcattgacg gaaatcgtca aaacctggca ggagcaaacc
     7021 gcagagctgg gaaaaacgta cccgtgggtg caggtctttg aaaacaaagg tgcggcgatg
     7081 ggctgctcta acccgcatcc gcacggacag atttgggcaa atagcttcct gcctaacgaa
     7141 gctgagtgcg aagaccgcct gcaaaaagaa tattttgccg aacagaaatc accaatgctg
     7201 gtggattatg ttcagcgcga gctggcagac ggtagccgta ccgttgtcga aaccgaacac
     7261 tggttagccg ttgtgcctta ctgggctgcc tggccgttcg aaacgctact gctgcccaaa
     7321 gcccacgttt tgcggatcac cgatttgacc gacgcccagc gcagcgattt ggcgctggcg
     7381 ttgaaaaagc tgaccagtcg ttatgacaac ctcttccagt gctccttccc ctactctatg
     7441 ggctggcacg gcgcaccatt taatggcgaa gagaatcaac actggcagct gcacgcgcac
     7501 ttttatccgc ctctgctgcg ctccgccacc gtacgtaaat ttatggttgg ttatgaaatg
     7561 ctggcagaga cccagcgaga cctgaccgca gaacaggcag cagagcgttt gcgcgcagtc
     7621 agcgatatcc attttcgcga atccggagtg taagaaatga gtctgaaaga aaaaacacaa
     7681 tctctgtttg ccaacgcatt tggctaccct gccactcaca ccattcaggc gcctggccgc
     7741 gtgaatttga ttggtgaaca caccgactac aacgacggtt tcgttctgcc ctgcgcgatt
     7801 gattatcaaa ccgtgatcag ctgtgcacca cgcgatgacc gtaaagttcg cgtgatggca
     7861 gccgattatg aaaatcagct cgacgagttt tccctcaatg cgcccattgt cgcgcatgaa
     7921 aactatcaat gggcgaacta cgttcgtggc gtggtgaaac atctgcaact gcgtaacaac
     7981 agcttcggcg gtgtggacat ggtgatcagc ggcaatgtgc cgcagggtgc cgggttaagt
     8041 tcttccgctt cactggaagt cgcggtcgga accgtattgc agcagcttta tcatctgccg
     8101 ctggacggcg cacaaatcgc gcttaacggt caggaagcag aaaaccagtt tgttggctgt
     8161 aactgcggga tcatggatca gctaatttcc gcactcggca agaaagatca tgccttgctg
     8221 attgactgcc gctcactggg gactaaagca gtttccatgc cgaaaggtgt ggctgtcgtc
     8281 atcatcaaca gtaacttcaa acgtaccctg gttggcagcg aatacaacac ccgtcgtgaa
     8341 cagtgcgaaa ccggtgcgcg tttcttccag cagccagctc tgcgcgatgt caccattgaa
     8401 gagttcaacg ctgttgcaca tgagctggac ccaatcgtgg cgaaacgcgt gcgtcatatc
     8461 ctgactgaaa acgcccgcac cgttgaagct gccagcgcgc tggagcaagg cgacctgaaa
     8521 cgtatgggcg agttgatggc cgagtctcat gcctctatgc gcgatgattt cgaaatcacc
     8581 gtgccgcaaa ttgacactct ggtagaaatc gtcaaagctg tgattggcga caaaggtggc
     8641 gtacgcatga ccggcggcgg atttggcggc tgtatcgtcg cgctgatccc ggaagagctg
     8701 gtgcctgccg tacagcaagc tgtcgctgaa caatatgaag caaaaacagg tattaaagag
     8761 actttttacg tttgtaaacc atcacaagga gcaggacagt gctgaacgaa actcccgcac
     8821 tggcacccga tggtcagccg taccgactgt taactttgcg taacaacgca gggatggtag
     8881 tcacgctgat ggactggggt gcgactttac tttccgcccg tattccgctt tccgatggca
     8941 gcgtccgcga ggcgctgctc ggctgtgcca gtccggaatg ctatcaggat caggctgcgt
     9001 ttctgggggc ctctattggt cgttatgcca accgcatcgc caatagtcgc tatacctttg
     9061 acggtgaaac cgtgacgctt tcgccaagcc agggcgttaa ccagctgcac ggcggcccgg
     9121 aagggttcga caaacgtcgc tggcagattg tgaaccagaa cgatcgtcag gtgctgtttg
     9181 ccctgagttc agatgatggc gatcagggct tcccgggtaa tctcggcgcg acggtgcaat
     9241 atcgtctgac cgacgataac cgtatctcca ttacttatcg cgccacagtt gataaacctt
     9301 gcccggtgaa tatgactaat cacgtctatt tcaatcttga cggcgagcag tctgacgtgc
     9361 gcaatcacaa gttgcagatt ctggcggacg aatatcttcc ggttgatgaa ggcggcattc
     9421 cgcacgacgg cttgaaatct gtcgccggaa cgtcttttga tttccgcagc gccaaaatca
     9481 tcgccagtga gtttcttgcc gacgacgatc agcgcaaagt gaaaggttac gatcacgcat
     9541 tcttgttaca ggccaaaggc gatggcaaga aagtggcggc gcatgtctgg tcagcagatg
     9601 aaaaattgca gctgaaggtc tacaccaccg ctccggctct tcaattctac tccggcaact
     9661 tcctcggcgg cacaccgtcg cggggaaccg aaccttacgc cgactggcaa gggctggcgt
     9721 tggaaagcga gtttctgccg gacagcccga accaccctga atggccgcaa ccggactgct
     9781 tcctgcgtcc tggcgaagag tattccagcc tgacggaata tcagtttatt gctgagtaat
     9841 gttaagccct cgtcatgagg gctttatctt catattgttc aaatcaccag caaacaccga
     9901 catatttgca actcaatatt cacaacaacc ttacactgcg ccactatttt cgctatggtt
     9961 atgcgtaagc attgctgttg cttcgtcacg gcaatataat gagaattatt atcattaaaa
    10021 gatgatttga ggagtaagca tatggctgta actaagctgg ttctggttcg tcatggcgaa
    10081 agtcagtgga acaaagaaaa ccgtttcacc ggttggtacg acgtggatct gtctgagaaa
    10141 ggcgtaagcg aagcaaaagc agcaggtaag ctgctgaaag aggaaggtta cagctttgac
    10201 tttgcttaca cttctgtgct gaaacgcgct atccataccc tgtggaatgt gctggacgaa
    10261 ctggatcagg catggctgcc cgttgagaaa tcctggaaac tgaacgaacg tcactacggt
    10321 gcgttgcagg gtctgaacaa agcggaaact gctgaaaagt atggcgacga gcaggtgaaa
    10381 cagtggcgtc gtggttttgc agtgactccg ccggaactga ctaaagatga tgagcgttat
    10441 ccgggtcacg atccgcgtta cgcgaaactg agcgagaaag aattgccgct gacggaaagc
    10501 ctggcgctga ccattgaccg cgtgatccct tactggaatg aaaccattct gccgcgtatg
    10561 aagagcggtg agcgcgtaat catcgccgca cacggtaact ccctgcgtgc gctggtgaaa
    10621 tatcttgata acatgagcga agaagagatt cttgagctga atatcccgac tggcgtgccg
    10681 ctggtgtatg agttcgacga gaatttcaaa ccgctgaaac gctattatct gggtaatgct
    10741 gacgagatcg cggcgaaagc agcagccgtt gcgaaccagg gtaaagcgaa gtaaacgtca
    10801 ttcgtttaaa aaatgagaaa gccgactgca agtgagtcgg cttttttgtt tgtcgttgct
    10861 aacaatggag cacattgcct gatgcgacgc tgcgcgtctt atcaggcctg tggtgattca
    10921 tcggatacgc cactctgacg gcgcatccga caattaaacc ttacccgcga cgcgctttta
    10981 ctgcattcac cagttgacgt aacagagcat cggtatcttc ccagccaatg caggcatcgg
    11041 tgatgctctt accgtaggcc agcggctccc cgctatcgag gctctgattg ccttccacca
    11101 gatggctttc caccatcacg ccaataatgg ccttttcgcc accggcaatc tgctggcaaa
    11161 cgtcagcaca aacatccatc tgctttttga attgtttgga cgaattagca tggctgaaat
    11221 cgatcatcac ctgagctggc agacctgctt tgttcagccc ttctttcact tcagcaacgt
    11281 gcttcgcgct gtagttaggc tctttaccgc cgcgcagaat gatatggcaa tcgccgttac
    11341 cactggtatt cacaatcgcc gaatgccccc atttagttac ggacaggaag cagtgcggcg
    11401 caccggcggc attaatggca tcgatagcca ctttaatcgt accgtctgtg ccatttttaa
    11461 aacctaccgg acaagaaaga ccagacgcca gttcgcggtg cacctgcgat tccgtggtac
    11521 gtgcaccaat tgcgccccag ctcatcaggt cagcgagata ctgaggagtg atcatatcga
    11581 gaaactcacc cgccgctggc agaccgctgt cgttaatatc aagcagcaat ttacgggcta
    11641 tacgcagacc gtcgttgatc tggaagctgt tatccatatg cggatcgtta atcaaccctt
    11701 tccagcccac cgtggtacgc ggcttttcaa aatagacgcg cattacgatt tccagctcat
    11761 ctttcagctc ttcacgcagc gccagcaagc gagtggcata ctctttagcc gcgacaggat
    11821 catgaattga gcacgggcca atcaccacca acaggcgatc atcattacct ttcaggatct
    11881 tatggatcgc ttttcgggca tgggcaaccg tattcgcggc attttcagtt gcggggaatt
    11941 tttccagcaa tgcgacagga ggaagtaact ctttgatttc tttgatgcgt aaatcgtcgt
    12001 tctgataatt catgtctgtt ccagtgttgc catacttatc ttagtgaatg caatacttcc
    12061 aatctatatc ttccgtcaga atgtgtaaac ggggttttac actatgaacg gattaatcct
    12121 ggaattagca agaaaaacgc cagattgtcg cgaaaaacga gatctctcct acaatttcta
    12181 actgtaactc ctttgtttgt taattatttc aagattctct gctgcgtttc ataaactggc
    12241 tgaaatctta aaccaatgcc ttatattcac ctgcaaatgc actgttggaa gaggttatcc
    12301 gacataacga ccataacagg agcatcctat gaaaatgaca aaactggcca cattatttct
    12361 gactgccact ctaagccttg ccagcggtgc cgcactggct gccgatagcg gagcgcaaac
    12421 taataacggc caggcaaacg ccgcagctga tgcgggccag gtagcccctg acgcccgtga
    12481 aaatgtcgcg ccaaataacg tcgacaataa cggggtaaat accggttctg gcggcacaat
    12541 gctgcattcg gatggttctt caatgaacaa tgacggaatg accaaagatg aagagcacaa
    12601 gaacaccatg tgcaaagatg gtcgatgtcc ggatatcaat aaaaaggtac aaaccggtga
    12661 tggcatcaat aatgatgtcg ataccaaaac cgacggtacc acgcagtaac cctgcaaaat
    12721 agccaataat gggagagcgg agtctctccc ttttaatttt tcacttctaa aagcagtatg
    12781 atgtcataaa gttaagtgag aataattaag ggatgactcg tcatggcgca ctcacactca
    12841 cacacgtcct cacacctgcc agaagataat aatgctcgtc gcttgttgta tgctttcggc
    12901 gtgactgccg ggtttatgct ggtagaagtc gttggtggtt ttctttctgg ttctctggca
    12961 ttgctggccg atgcgggtca tatgttgacc gatactgccg ccctgctttt cgccctactc
    13021 gccgtgcaat tttcacgtcg tcctcccacc attcgccaca ctttcggttg gctaagactg
    13081 accacgctcg cggcgtttgt gaatgccatc gccctcgtgg taattaccat tttgattgtc
    13141 tgggaggcga tagaacgctt ccgcacgccg cgtccggtcg agggaggcat gatgatggca
    13201 attgccgtgg ctgggttgct ggcaaatata ctttctttct ggttacttca tcacggcagc
    13261 gaagagaaaa acctcaacgt acgagcagcg gcactgcatg tgctcgggga cctgctgggt
    13321 tcggttgggg ctattattgc cgcgttgatt attatctgga ctggctggac acctgctgac
    13381 cccattctct cgatactggt gtcgcttctg gttctgcgta gcgcgtggcg actgttgaaa
    13441 gatagcgtga atgaattact tgaaggtgca ccggtatcgc tggatatcgc tgaactgaag
    13501 cgtcgtatgt gccgggaaat cccggaagtc cgcaatgtgc accatgtaca tgtatggatg
    13561 gtaggcgaga agccggtgat gacgttgcat gtgcaggtga tcccgccaca cgatcacgat
    13621 gccttgttgg atcagatcca acactacctg atggatcact atcagattga gcacaccacc
    13681 attcagatgg aatatcaacc ttgtcatagg ccagactgcc acctgaatga aggggtgtct
    13741 ggccattcac atcatcacca ttaatgggac agcgcgcgtg agcctctttc acgtgcgctg
    13801 ttgatccaca tccggctgcc gttgagcgca ataaaggtca ggatgatgta ctccagagac
    13861 atggcgtaaa cgccctgaag tgcaaaaata acgacgctaa tcacgttaat aatcacccac
    13921 aacagccagt tttccacata cttacgcgtc atcagaatca ttgccacgat agataacacc
    13981 atcatgcatg aatcccagaa cgggaaagcg tccggttgca gttcaggcat cacaacctgt
    14041 aatcctaatg cttgcatgat catgactgcc acgcgggtca aaaatgcaaa caccggattg
    14101 ataaataccg tcatcagacc aatcgaaaca acgcaaaccg ccaaccagct gagtgccttc
    14161 ggcaatggca accagcgaat tttcaactcc gcctcgttct gactggtttg tcgcgaccac
    14221 gcataccagc cgtaaatatt cgcggcaaag aaaaacacct gtaatagcag gctggcatac
    14281 agctgaatct gaaagaaaat aatgccaaac aaggtaacgt tgatcaggcc aaagaagtag
    14341 ttgctgatct tctccagact ggcaaggcca atacacagca acccggcgat cgtgcctacc
    14401 gcttcgatcc atgagagatc ataaccgccc gcccctattg gtatatgtac caggatattc
    14461 tgcacactaa aaaaatccat ctttccccca aagcgtaacg ttacgcctta ttattcgtta
    14521 tccacgtagt gtagccgcaa aatccagcat acgattgagc ggcaccagcg ccctctctcg
    14581 cagcctctca tcgacatgaa cctcgtgatt acttccttcc tgttctaatg cctctgcgat
    14641 agcctgaagg tcattcatgg ccatccacgg acaatgcgcg cagctgcggc aggttgcgcc
    14701 ctcacccgcg gttggtgctt ccagtaactc tttatctggc accgcctgct gcattttgta
    14761 gaaaataccc cgatcggttg ccacaataag cctctgatga ggcaatgctt ttgcagcagc
    14821 gatcagttga ctggtggaac cgaccgcatc cgccatatcg acaatagctt gtggtgattc
    14881 tggatgcacc agtatggcag catccgggta ttcttcttgc aagcgggtta acgcctgagt
    14941 cttaaattca tcatgcacaa tgcaggcacc ctgccagcat aaaatgtctc cacccgtctg
    15001 tttttgcacg taacgcccca gatgtttgtc gggtgcccag atgatttttt cacccaaact
    15061 atcaagatga tcaataagtt cgacggcaat gcttgaagtt accacccaat ctgcgcgcgc
    15121 ttttaccgca gcagaagtgt tggcgtagac gacgacagta cgatcgggat gggcatcgta
    15181 aaatgcgtta aattcttcaa cagggcagcc gagatccagt gaacactcaa cctgaagtgt
    15241 cggcatcaga attgtttttt ccggactgag aattttggcg gtttctccca taaatctcac
    15301 cccagcgact aacaaagtag aagcgggatg ctttgcaccg aagcgcgcca tttccagaga
    15361 atcagaaata cagccaccgg tttcttctgc cagttgttga atttcgggat cggtatagta
    15421 gtgggcaacc atcaccgcat tacgttcttt tagcagacgt tttatcttct cgcggtaata
    15481 cgctttttca tcaatgctta acggcgtcgg cttcgggggg aaaggataaa tcgccgtgtc
    15541 tggatcaaac attacgctca tcttaccatc tcgttttaca ggcttaacgt taaaaccgac
    15601 attagcgtaa cattcgctgt tatcggtgta atttgtttta tatgctaaac aagatagtcg
    15661 aatggtgggg aaaagtcact cgaattacgc ctaattccac cgacgtagag ttgcttgcac
    15721 agtaagctct gacgatgtca tcaggtttac aaagtgaaat tgaggatgcc tgaatagcaa
    15781 aaaagcgcct ttagggcgct tttttgcatt ggtgggtcgt gcaggattcg aacctgcgac
    15841 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccgagtg gtgggtgatg
    15901 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
    15961 acccgatact gcgctggata ctacaaacta cttccaccga acccaccgta attagtggtg
    16021 ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc taccaactga
    16081 gctaacgacc cggtggtggg tgatgacggg atcgaaccgc cgaccccctc cttgtaaggg
    16141 aggtgctctc ccagctgagc taatcacccg acactacgct ggatactaca actaactatg
    16201 ttagtggggg tcgtgcagga tgactcgact tcgtctcgcc cttcggcgcc gttgctgatg
    16261 caacgttatc cttcacgttt tactaccgct tccaccttag cgattggtgg gtcgtgcagg
    16321 attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc
    16381 actttttcgt tgctttcggt ttgtttgata tcccgtggca acggcggcat atattactga
    16441 tttcagactt gagcgcaaca aaaatttcga tgcagatcac tcaactgctt atgattcgca
    16501 cgacacgacc agaaataatg cgacttctgg tcgtgtgtta tgcattacat cgcgttcaga
    16561 cgtttttgtg cctgtttagc gccatcagta ccagggtatt tactgataac ctgctggtat
    16621 acggctttcg cttttgcggt gtcacctttg tcctgcatga tgacgccgac tttaaacatc
    16681 gcatcagcag cctttggtga cttcggatag tttttcacta ccgaagcaaa atagtacgcc
    16741 gcatcatctt ttttaccctt gttgtagttt aactgaccca gccaataatt ggcgtttggc
    16801 aggtaagttg aatcagggta atttttgatg aaattctgaa atgccaccat tgcgtcatcc
    16861 tggcgggatt tatcctgcac cagcgcaata gctgcattgt aatccgtgtt tgcatcgccg
    16921 cttttcaccg gcgcgccagc attcgcagta ccagcatcag ctgtcggcgt cgttgatgcc
    16981 gtcgcaccgc tttgatcgct gctggttgat tgcgccgctg caccaccgct gctgaggctg
    17041 tcgatctgca acaggatctg cttttgccgc tccacgacct gattcagttg atactgattt
    17101 tcctgaatct gaccacgcag ggaatcaata tcggattgat tatcagaaag ttgttgctgg
    17161 agttgggtta aaagctggct gtgagcatta gaaatacgct caagttgagt gacgcggtct
    17221 tcgaccgagc ctgagctgac actactgatt ggtgcctgag caaaagcggc ccagggggcc
    17281 gctataccaa ccagtaacga cagactcaat agttgatgtc tgaagttact gctcatgcaa
    17341 ttctcttagt aaaccagtac cgcacgacgg tttttggagt atgccgcttc gtcatgaccc
    17401 agtactgcag gtttttcttt accgtaagaa acgatggaga tctggtctgc agaaacgcct
    17461 ttaccctgca ggtacatctt aacggcgttc gcacgacgtt cacccaggga gatgttgtat
    17521 tccggagtac cacgttcgtc cgcgtgacct tctacggtga ctttgtaaga cgggttgcta
    17581 cgcaggaagt ttgcatgtgc atccagcatt tgagcgaagt cagaacggat atcgtacttg
    17641 tccagatcga agtaaacgat gttgttctgc tgcagctgtt gcatttgcag acgagcctgc
    17701 tcttcggaag acatgttgcc gttgccgccg ttcgcatcca taccagtgcc ggcacccagc
    17761 atgccttcgc tgccgtcatt gctggcgttc ttgttggaag aacatgccgc aattgccata
    17821 acaggcagag caatcatcag ccctttcagc actttgttca gttgcatttc aatgattcct
    17881 ttactattca attaattatt atcacagata cggcgaccag gcagggaatt tgacctgtcc
    17941 atcagttgcc ggaagacgcg ctttgaaacg cccatctgta gaaaccaaat tcagcacgga
    18001 tcccatcccc tgagaagagc tgtagattac catagtgccg ttaggtgcca gacttggcgt
    18061 ttcatccagg aacgtggacg acagaacttg tacgcctccc gttgccagat cttgtttggc
    18121 aatgtgctgc tgcccaccat tggagctgac cattaccata aatttaccgt cgctgctgac
    18181 atccgcatcc tggttctgcg aaccttccca ggtaatacgt tgtggcgcac cgccgttgat
    18241 attcacttta taaacctgcg gacgaccggc ctggtcagaa gtaaatgcca ggttctggct
    18301 atccgggaac caggtcggtt cggtattgtt actgcgacca tcagtcacct ggcggatctg
    18361 accagaagcc aaatccatta cgtacaggtt cagactaccg gttttcgaca aggcgaatgc
    18421 cagtttgctg ccgtctggcg agaatgcagg tgcaccgttg tgacgcggga atgaagccac
    18481 ctgacgtaca gcgccatttg ccagcgtctg aataaccagc gcggaacgac cgctttcgaa
    18541 ggtcacataa gccagtttag aaccgtctgg tgaccacgcc ggagacatca gcggctgcgg
    18601 tgaacggtga acgacaaact ggttgtaacc gtcatagtca gatacgcgca gttcatacgg
    18661 gaactgaccg ccgttggtct gaacaacgta ggcaatacgg gtacggaacg cacctttaat
    18721 gccggtcagt ttttcaaaca cttcatcact ggcggtatga ccagcataac gcagccactg
    18781 cttgttcact ttgtacgagt tctgagcaag tacagtaccc ggtgcgccgc cagtgtcaac
    18841 aagttgataa gcaacattgt aagaaccatc cggattcgga gtgacttgac cgacaactac
    18901 agcgtcaatg cccagtgcgg accatgcagc tggttgtact tcctgcgcac tacccggctg
    18961 ctgtggcaga cgagcgcgat ctaacggatt aaatttaccg ctgttacgca agtcagcagc
    19021 aacgatgccg ccaatatctt caggtgccgc accaggcccc gcccactgga aaggaacaac
    19081 accaatagga cgaccggaat ctacaccgct gtcgatcaca atgcggactt cagcatgcag
    19141 aactgatgcc cacagtatga gaaaaccaaa tgctactcgt aatgcctgct tcatcatatc
    19201 tcccttatct ggaccgatgg cccacgataa tttagcagaa tgttaacaaa ctaaaataca
    19261 caaaactacc agaaccccgt ggcaaccggt gcctgatgtt gaccgtccga acagtcaaca
    19321 tcgcgattac ggtttgaagt ccaatggcgc gtttttgaac acttcatata ctgcctggct
    19381 tggtggtttc gggatcttcg caagtttagc tgctgccaac gcagcctgac aaagtgcggg
    19441 atcgccacct tcaggtttga tatccagtaa cataccatcg ggtgccagtt ttatgcgcag
    19501 cgtacaggtt ttgcctgcat aggacgatgc gtcatagaac ttactttcga tagcagattt
    19561 aatctgcccg gcatagttat tgatatcggc ccctgatgcg ccattgtttt tagtattacc
    19621 actcccggca ggcgaagcat tgttcccttt cgcccctccc cccgttttcg gtgcattctt
    19681 accagagctt agctcaccga aaatatcatc tgcctctgcg gcagcttttg ctgcagcggc
    19741 tttttcagct gctgcttttg ccgctgccgc ttttttgtct gcggcggctt tttctgccgc
    19801 tgctttctta tcagctgctg ccttatcagc agccgctttt tctgctgctt ttttgtcggc
    19861 tgcagctttc tctgctgccg ctttcttatc agctgcagcc ttttcagcag ccgctttctt
    19921 ctcagcttct gccttggctt tttcagcagc ttcagttgcc gctttctttc ttgcttcagc
    19981 tgcagctgct tctgccgctt ccgctttctt cttcagtgcc gcagcggcta cctcggcttt
    20041 tttctgcgct tcggctgcgg ctttggcggc ttctgcttct gcttttttct tcgcgtctgc
    20101 agccgctttc ttcgctgctt cttccgcagc tttagcatct gccgccgctt tcgctgccgc
    20161 cacttcagct tgcttctgct ttaactcggc ctgttttgcg gcttcttcag cctgcttttt
    20221 ctgctcctga gccgctaacc gctctttctc aagttgcttc aggcgttcct gttcagccgc
    20281 ttgtttctca cgcagttctt cagcagcctg ctgttccttc atcttgcgct gttcatcaga
    20341 acgcttcgcg cttgattcct ggctttgcat gcgtttgtac tgctcaacta ccgcacctga
    20401 atcaaccatg acagcgtcga tggacgaacc accgccgcct ccggctgaag cttctatatt
    20461 ctcatcgaac gaactccaga tcagcgccgc aaataagatg acatgcagca ctgctgaaat
    20521 aattatcgcc cgcttgagct tgtcgttttg ttcggttgcc tttgacactc tcggtttcca
    20581 aaaactgttc gcctgttacc cgctctcttt caagcaaggg aaacgcagat gtttagatag
    20641 gctgcgtcat taaaccaacc gatttcacac ccgcactatg taacaagttc agtgctttaa
    20701 ttatttcatc gtaaggcaca tcttttgcgc caccgatcag aaagaccgtt ttcgggttgg
    20761 ccttgaaacg gctggacact tccgccacca cctgctctgg tggtaaacgc tccaggcgat
    20821 ctttctcaac caccacggtg tactgaccaa taccagacac ttcaacaatc actggcggat
    20881 tatcgttact gctcaccgcc tgtgattcag tagcgtctgg cagatcgacc tccacgctct
    20941 gggtgatgat gggcgctgtc gccataaaga tcagcaacag caccagcagt acgtccagca
    21001 acggaacaat gttgatttcg gacttgagat cgcgacgacc tcgtccacgc gctctggcca
    21061 tggcttaccc cttgttgctc tcgctaacgg taaacgcctg gcggtgcaga atcgcggtaa
    21121 actcttccat aaagttgtcg taattcagtt ccagtttgtt tacgcgctgg ttgagacggt
    21181 tataagccat taccgccgga attgcggcga acagaccaat tgccgtcgca atcaacgctt
    21241 ctgcgatacc gggcgcaacc atttgcagtg ttgcttgttt taccgccccg agggcgataa
    21301 aggcgtgcat gatcccccag accgtaccaa acagaccaat atacgggctg atggagccaa
    21361 ccgtaccgag gaacggaatg tgcgtttcca gattttcaag ttcacggttc atggagatac
    21421 gcatagcacg cgacgcccct tccactacgg cttccggcgc atggctattg gcacgatgca
    21481 ggcgcacaaa ctctttgaac ccgctgtaaa agatttgttc cgaaccagtc agattatccc
    21541 gtttcccctg actctcttga tagaggcgag agagttcgat tccagaccag aatttatctt
    21601 caaacgcttc ggcttcacgc gccgctgcgt taagaatacg ggttcgctgg ataataatgg
    21661 cccaggatgc gattgaaaaa ccaatcaaaa tcaacatgat aagtttaacc agaaggctag
    21721 ccttcaggaa caaatcaagg atattcatgt cagtcactgc ttaaactccg cgacaataga
    21781 cttgggaagc gcacgaggct tcattttgag tgggtcaacg caaacaacca gaacctctgc
    21841 ttcattcagc aaagtattct cggcgttgac aatacgttgc gtgaaaacca aagaggtgcc
    21901 acgcattgat gttatttcag tctgtatttc gagcatatcg tcgagccgcg caggtgcgta
    21961 atattccacc gtcattttac gtaccacaaa ggcaacgcgt tcagccatca gcgcctgctg
    22021 actgaagtga tgatgacgca gcatctctgt gcgtgctctt tcataaaaag cgacgtaact
    22081 ggcgtggtac accacaccac cggcatcggt atcttcatag tagacgcgaa ccggccatcg
    22141 aaacagcgtt gtattcactt tacatcccgg taatgcaaca aaagttagag cttttaaacg
    22201 ctgctactat acgcgcgcga ttgaggtttg ggaatgaggg caagttaagg gcgcgtaaaa
    22261 atttatgggc ctagagcggc ccataatttt ttaaagagaa ttctgattta aaagaagaag
    22321 aaaatcagcc cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaat cccttgccaa
    22381 agaaccttct gcggacgaaa gcccacgccg tgaatcacac cagcacagac ggcccacatc
    22441 agcaataaac catgccagat ttccagttca ctggtcttcg cggcaaaacg agatgggtcc
    22501 caaaacatac atcctgctaa cagaagcgcc atcacgaagg aaagcgcccg cagggggcgc
    22561 ttgtccatta ccgcatacaa ggtcgcgata atcttactca tcagatgtct tcttgaccgg
    22621 ctttgcctga ttcgacgtgt tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa
    22681 gaagcgttcc cagaatccat gcgaaatacc acatttttag ctccttactt agtacagaga
    22741 gtgggtgtta cgttcaatat cttctttggt gatacgaccg aacattttcc agtaacacca
    22801 ggcggtgtag agcagaatga tcggtaccag aaccaccgca acccaggtca tgacgttaag
    22861 cgtcagctgg ctggaagttg catcccacat tgtcagactt gcgttcatca tggtgctgga
    22921 cggcatcaca aacgggaaca ttgcgatacc ggctgtcagg atgatgcagg ccagcgtcag
    22981 ggaggagaac acaaacgccc acgcggcttt atccatacgt gcagtcagga tggtcagcag
    23041 cagcagaacc acacccagtg ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac
    23101 cagccatgcg ccagcttcac gaaccacttc tttattcagt gggttagagg ctgcgtaatg
    23161 gtccattgtc gatttcacga cataaccatc gataccgtac atcacccata cgccagccag
    23221 tgcgaaacag accagtgtca ccagcgcagc cacctgagcc gttgcacggg tacgcaggtg
    23281 cagttcgccc acggtacgca tttgcagata ggttgcgccc tgagtaatga tcatccctac
    23341 gctcaccacg cctgccagca ggccgaacgg gttaagcaac tggaagaagt taccggtgta
    23401 gtacagacgc agatattcat caacgttgaa cggtacgccc tgcaacaggt taccgaacgc
    23461 tacaccaatt accagcggcg gaacgaagct accaatgaag atgccccagt cccacatgtt
    23521 acgccagcgg gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa
    23581 agacgccagc acgaggatca tcgccacata gaagccggag aacgcagcgg catagaccat
    23641 cggccaggca gcaaagagtg cgccgcccgc ggtgatcagc caaacctggt taccgtccca
    23701 gtgtggtgca atggagttaa tcataattcg acgctcggtg tcgttacgac cgaggaaacg
    23761 ggtgagcatg cccaccccca tgtcgaaacc gtcagtgact gcaaaaccaa tcagcagaac
    23821 gccaaccagc agccaccaga taaaacgcaa tacttcataa tcgatcattt gacgactcct
    23881 gtcttagcgt gccggctgag tagtcgtgga agactgctca aagtgatagc gaccggtttt
    23941 caggctgctt gggccgaggc gtgcaaactt gaacattaag aacaattctg ccaccaggaa
    24001 cagggtatac aggccgcaaa tcagcaccat tgagaagatg agatcgcctg cggtcagtga
    24061 cgaattcgcc acagctgtcg gcagcacttc accgatagcc cacggttggc ggccatattc
    24121 agccacgaac cagcccgctt ctacagcaat ccacggcagc ggaataccgt acagcgcggc
    24181 gcgcagaagc cattttttct cgccaatgcg gttgcggatg acactccaga aagagagcgc
    24241 gatgattgcc agcagcagga agccacacgc caccatgata cggaacgcaa agtacagcgg
    24301 cgctacacgc gggatggagt ctttggttgc ctgttgaatc tgcgcttcag tcgcatcagc
    24361 cacgtttggc gtatagcgtt tcagcagcag accgtaaccg aggtctttct tcatgctatt
    24421 gaactggtca cgaaccgtct ggtcggtaga accagaacgc agttgttcga gcagagagta
    24481 cgccttcatc ccgttacgaa tgcgttcttc atgctgcacc atcagctctt tcaggccgat
    24541 aaccggggta tccacggaac gcgttgcaat gatgcccagt gcgtaaggga tctgaatcgc
    24601 aaatttgttc gtctcctctt cctgatcagg aatgccgaac aaagtaaagg cagcaggcgc
    24661 aggttgcgtt tcccactcgg cttcaatagc agccagtttg gttttctgca cgtcgcccat
    24721 ttcgtagccg gattcatcac ccagaacaat aacagacaga acagcagcca taccgaagct
    24781 ggcagcgata gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca gcatatacca
    24841 tgcgctgata ccgaggatga acatcgcgcc agtcacataa ccagacgcta cagtgtgaac
    24901 gaatttcacc tgagcaaccg ggttaagcac cagctcggag aagctcacca tctccatacg
    24961 catagtttca aagttgaaat cggacgcgat tgggttttgc atccagccgt tcgcaaccag
    25021 aatccacagt gcggacaggt ttgaaccgag cgccaccagc caggtgacac acatatgctg
    25081 aactttaccc agacgatccc aaccgaagaa gaacagacct acaaaggtgg attcgaggaa
    25141 gaaggccatc agaccttcga ttgccagcgg cgcaccgaag atatccccta catagtggga
    25201 atagtaagac cagttagtcc cgaactggaa ctccatggtc agaccggtag ccacacccag
    25261 agcgaagttg ataccaaaca acttgcccca gaacttggtc atatctttat aaatctgttt
    25321 gccggagagg acgtagaccg tttccataat ggccaggagg aacgccatac cgagcgtcag
    25381 tggcacaaaa aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg acagttcgac
    25441 tatatctaac atcatgactc cttgctcatc gcatgaagac tccgagagtg aaccccgtta
    25501 gaaagggtca cacgcatgcc ccaatagaaa tttgtttgaa cactttcatc ataccatgtg
    25561 aatgcacaca aaaacggcgt aaagaaaggt tgcaaacatg ttaataaaaa ctcaaattga
    25621 tcccacgtat atattacgcc gcaaaatcct tacaataaac aggtttttgt tgaagcaaat
    25681 ttgcattttt cgacggtgat caatttatag ctaaattgcc gcctttcagc caatttgatc
    25741 gagaacaatt tatctctttt tgatgcccat ttccaagact tatacattga taaatatcaa
    25801 tttctacaaa ttatgttaat catgtttcaa caaggtaact ttagtgatat ttatgtttaa
    25861 aatgtgtgaa ttataagact gaaattagtt tcggttggca attaaagata aaaagagacg
    25921 aagcaaaaac agcacggttg accttaattc tttttttggt cactggctaa tacgacgcag
    25981 agtttcagga ttattaataa aggcataaat ctccctcccg ccgatattaa catttaatta
    26041 acttataatt atattataat taactcaccc gtggttatta ttttgttaaa aataaagcca
    26101 catatacgta gcttgagagt gaagataaac acaaaattac ctgatgcgct acgcttgcca
    26161 gtgtctacat catctctgca atatattgaa tttgcctgct tttgtagacc gaataaagcg
    26221 ttcatgccgt atccggcatg aacaaagcgc cctttgtcaa caatctggcc gcgcatatgt
    26281 ggcttcggtt tgattgctat tcaggcaagc cggtaactga acgtccgtga ctggccgggt
    26341 aaaaaaggcc ccgatagatt tgaaccttga ttttcctcgg tggtaatgtg ttcatccatc
    26401 atcgtttctg agcaagaaat cacctcgcga ctgaaagcaa cagtcgcatc acaggttgct
    26461 gattcagccg gattaaacag ccgtaaaatc acttcttgtc ggtcttcagc tttcttaagt
    26521 gcgcttatca ggcatcccac tgggggcatt ttcaacaaac tataactttc cggcacgttg
    26581 aatccggctt tgttgagctt catcgcatcc catgggattt tgttgtaaca ctgtactgga
    26641 gtcagccatg ctcgcgcctg ctgagctaca ccagcggcgg ttggcgtacc ggtataactc
    26701 aataaactta ggcgacaaga aagcagacca cgtagttgtg agtccgggac tggcatttta
    26761 attcccgaag gccgcctagg ccttaaaagc agatcttctt tccccagtaa gcccacgccg
    26821 cgcagcaacg taatggcaaa ggttttcttc tcttcaccga tgacttcaaa ttcacgtagc
    26881 ccttcgctaa agacagccat gccgttacgc ccttcctgta aggctgcata gttgagcata
    26941 ttccataccg gaaccggcgc ttctttccag ctttcttgct gccagttgtt cattgcactg
    27001 tcgttcacgg ggcgcgttag cgaaccaaac tgggtatctg ccagaacact atcggtgtta
    27061 aaagatgtag ggatcaggac gcgaaggcga tgatcgtcag cctggttatc aaggttgata
    27121 tccacatcaa tgcgcctgtt gttatgacta agagtgacaa ccaactcgac atctactctg
    27181 ccagtggatt gtcttgcgct gcgttgttgc agattacgcg gcaccgccat ttcatagcgg
    27241 ataacagccc tgctctgcca ggcctcatga ataatctcgc attgcggttt cgcattcgcc
    27301 gaagtaatta cccactcttc ttttgcgggt gaatagtcat attcatcacc atcatcagag
    27361 ctttcttcaa tttgcaacac ccggtcatag cgcacaccgc tatctttatc taccagttgt
    27421 agcgttccat cctcattgag cgcaatttgc cagaaagcat tttccagtat cccttcagcg
    27481 tcactttttg ccgcaatcac gttgccaggc tgattcgctt cgatataaag cgtgcgatag
    27541 cccatagaag ggacaatctg gttgatctgt atatcaaact ccataaaggg atcgtaatta
    27601 ccgtaatgaa ctatttgccg atcgattagg cctggatcga tctcacgggc atggcgaata
    27661 aaatacggta caggctgacc gcgatcgtct cgtagattaa actggctggc gcgcagccgc
    27721 acagtggtgt tgatcacttc ttcccgcggc cagggcatca ggttaaacag gacgagtttg
    27781 tcggcgtcgc tctgcggcat gttgtcggcg attttacgca tatagaaacg cagcagatta
    27841 tccgccatgt cttcagccag ttcgaagcgg gcgacgattt cgcgatgaac tttgtcacga
    27901 cagcagcagc cgatgctgtc gtgggcatga ttttttaaga tctctttcca cattttctcc
    27961 agcaagccgt ggtggtatcc aaaacccaac gtcctggcca gcgttgccag cggttccagc
    28021 agattaacaa tcttattttc aatacgtgcg tgggcaactt tgatatccat gcgcgtggaa
    28081 ccgatggtgc gatgcacgcg catatatttg ccatcaataa attccccttt cagggttgcc
    28141 agattatctc gctgcgcttc gatcttctca aatacctctt caaagcggct catcacaaac
    28201 tttcgttgag gataaatttc gcgcagtttc tccatcactt cgaagatatt ttgctgcaat
    28261 ggcatctgat catgcccatt cggcagcaaa atctctttgg ttacagacgc tttttccagc
    28321 acgtcaaaat aactgtcgag gcgtttgcgt aatccgtttt cgtccgcagg taagtactta
    28381 ccgatggcat aacccaacgg catcacctgc gccgtcactt cgctaccgtc actgctttgc
    28441 cacaaaaact cggttttatc cgtaccgtgg cgctcagaac atccacgcca gaacatggtg
    28501 caggtaatgc caaatccatt gtagatatgc ggcagttgcc cggacatgcc aaaggaatcg
    28561 ggtaaataac ctattttcat cggctcgcca aacgcgaggc agtcacgcat tccgtacatc
    28621 agattacgga cgatggattc cgcagaaaca atcgtggtat cggtctgggt ataccagggg
    28681 ccgataatca acttgccggc ttctaccagt ttcttcaagc ggtctttgtt ttccggtttc
    28741 accgcgaaat aatcttcgag gatcgccgtt tgcccgtcga gtacgtaata tttgtattcg
    28801 ttgtcctgtt ccagtcggca caggatctct tccatattat tgaccagcag aatgcgtgac
    28861 tcttcggtgg tgaaatacca ctcccgatcc cagtgcatat gcggggtgat gtgaacgcga
    28921 gataccgctt tcatcgtcgt ttcctttttg tttatggcat tacgccatca gtcagatagt
    28981 tgccatgctt aaccgcgtga cgccgccaga tcaggagaat tgcagtcgag attgcagccc
    29041 ccaccaatgc cgcaccaaac cagccaattg ccgccataac accgcccgcg ccattatcat
    29101 gaagtaaaaa gagcgagaaa atgccagcac cgggtgtcga aaggccgata ttcatcgcac
    29161 cgacaatagc gcccgttacc atagagccca gcacaaacga accaataacc cgcagcggat
    29221 cttcaatcgc catcgggatc gccccttcgg taatacctgc cagccctaac agccaggtgg
    29281 atttcccggt ctcaatttca aactctttaa acaggcgcgg tgcgagcatc gtggaagcgg
    29341 ttacggtaaa tgccgaaacc attttgacgg aggcgaaaat ggcatacggg ccgtaaacgc
    29401 cgttcgccat tgcgcccagg cagaatgcat aagcggcttt attcactggc cctccaaggt
    29461 caaaggaaca cataaaaccg agaatggcac ccagcaacag cgcgttactt cctgacagac
    29521 cgttcagcca ggcggtaagc gagttattga tccaggcgac aggttccccc accacaaaca
    29581 gcatcagact gcccgctccc aatgtaccga gcaccgggta gagataaaaa gtcaggaagc
    29641 cattgaattt actgctaaga cgcaagtgat ttttcatcca gcgtatcaag taaccggcta
    29701 tcaatccgcc aacgaccgcg ccgagaaacc cggagccgat catgttggcg gcaagtccag
    29761 ccgcaaagcc tggcgctaac gccggtttat ctgccagcga ataggcggta taggctgcca
    29821 gcaccggtac catcaaaatt ccgagcagcc cgccgcccag tttgcggtac atccacagcc
    29881 aggagttttc ttcattaaac agatcttgta gcccgaagat ttgcgacagt aataccgcga
    29941 ccgccagcac cgtccccccc gcgacaatca acggtacggc aaaagagatc ccgctcaaca
    30001 gtgcctgttt cagctccgtt ttgacgcttt tcaccggttg cgtatcttgc tgtaaggtac
    30061 gcgtctcatc gccacgctcg agggtaagcg attgttggat caacgcttct gcatggcgaa
    30121 tcggctcggc aacaggcaca gaaagcgcag gaatgccgtt aaaacgctca ctctccttga
    30181 tggcgacttc agccgcaaaa atacaggcag ttgcactatt gagttgatcc gccgttaaac
    30241 gcccttcaat accgttagca ccttgctttt caacgtagac attcacgccg agtttgcgtc
    30301 cggctttttc cagatactcc gcagccatgt aggtgtgagc aatacccgcc gggcaggccg
    30361 ttacgcaaac gatagttggc gcgttggaaa aagaggcaga aggttgcgtg cctcccttgt
    30421 catccagcgc cgagagcaat tcatcaggcg tcgtcgccga ctgtatacgc gcccgaatct
    30481 catcatccgc caggcgcgtg gtcagcgctg tcagcagttg catgtgcgta gtacccgctt
    30541 cattgagggg aatcgccagc aacaccacta aatcaaccgc ttccgggcca tccacgcctt
    30601 cccactgaag cggctcgctg agtgtggcga ccgcaaacgc cgcttctttt accgcagcag
    30661 ttttgccatg cggcacagcc aacccttcac ttaaggcggt cgggccaagg ctttcacggc
    30721 gatacacttc tttcagaaat tgctcagtac tggaaatttt ccccagagca gcaagacgtt
    30781 gagtcaacgc gtgaatggcc tcttcacggc tggtaaagcg cgcattcaga cacaacgcat
    30841 cgcggtgggt cagagtcgtc aggttcatca tgatcctcac ttgatcgttt ttatagtcgc
    30901 gccagtgtgc ccgacaaaac aataccattg cgtaatcaat ttcacttgat tgaaattatt
    30961 tgcattttat ttgtattgtt tacaggatga gtttatcggg cataatggct aaattcgtat
    31021 taatgagatg ctaaataatg ggacacaaac ccttataccg acagattgct gatcgcattc
    31081 gtgagcaaat tgcgcgtggc gagcttaaac ctggcgatgc gttacctacc gaatcggcat
    31141 tacaaacaga gtttggcgtc agccgggtta cggtgcgtca ggctttgcgc cagttagtcg
    31201 agcaacagat cctcgaaagc attcagggca gcgggactta cgtcaaagaa gagcgggtca
    31261 attacgatat ttttcagtta accagttttg atgaaaaact gtcggaccgt cacgtcgata
    31321 cacacagtga agttctgata tttgaagtga ttccggctga cgattttctt cagcaacagc
    31381 tacaaatcac cgtgcaggat cgtgtttggc atgtgaagcg cgtgcgctat cgcaagcaaa
    31441 agccaatggc gctggaagaa acctggatgc cgcttgcgtt gttcccggat ctcacctggc
    31501 aggtcatgga aaattcgaaa tatcacttta ttgaagaagt gaagaagatg gttatcgatc
    31561 gtagcgaaca ggaaatcatt cctctgatgc caaccgaaga gatgagccgt ctgcttaata
    31621 tcagccagac aaagccgatt cttgaaaaag tatcgcgtgg atatttagtt gatggtcggg
    31681 tgtttgaata tagccgcaac gcttttaata ccgatgacta taaatttacc ctgatagcgc
    31741 agcgaaaatc atcgcgataa gcacaaaaag gccatcatac gatggccaac catgtcgaaa
    31801 ccgacagccc ttcgccattc ctgtagaggg aagcggcgag ggctatttct tattacagat
    31861 atttatttca gaacagtttt cagtgcttca ccgatatccg ccaggctgcg aacggttttc
    31921 acgcctgcgg cttccagagc agcgaatttc tcatccgcag tccctttccc accggcaatg
    31981 atcgcacccg cgtggcccat acgtttgcct ttcggcgcag tcacaccagc gatgtaaccc
    32041 acaactggct tggtaacatg ctctttgatg tacgctgccg cttcttcttc agcgctaccg
    32101 ccgatctcac cgatcatcac gatcgcttcg gtctgcggat ctttttcgaa catttcgaga
    32161 atgtcgataa agttagagcc agggatcggg tcaccgccga taccgacaca ggtcgactga
    32221 ccgaaaccgt aatccgtggt ctgtttaacc gcttcatagg tcagtgtacc ggaacgggaa
    32281 acgataccca ctttacccgg tttgtgaatg tgacccggct ggataccaat tttgcattca
    32341 cccggggtga taacgcctgg gcagttcggg ccgatcatac gaacgcctgc ttcgtccagt
    32401 ttcactttca cggtcagcat atccagcgtc gggatgcctt cagtgatggt gataatcagt
    32461 ttgatgcctg catcgatagc ttccagaatg gagtctttgc agaacggtgc tggtacgtaa
    32521 ataacagacg cggaagcgcc agtagcagca acagcttcac gcacggtgtt gaacaccggc
    32581 aggccgaggt gggtagtgcc gcctttacct ggagttacgc caccaaccat tttagtgccg
    32641 tatgcaatgg cctgttctga gtggaaagtc ccctggctac cggtaaagcc ctggcagata
    32701 accttggtgt ttttatcaat taaaatggac attatttccc ctccactgcg gcaacaacct
    32761 gctgagctgc atccgtcaga ccttttgctg caataatatt caggccgctg tcagccagtt
    32821 tcttcgcgcc gagttcggcg ttgttacctt ccagacgtac cacgaccggt acgttaacac
    32881 ccacttccgc taccgcgccg atgataccgt cagcgatcag gtcgcaacga acgataccgc
    32941 cgaagatgtt aaccagaacg gctttcactt tgtcgtcaga gaggatgatt ttgaacgctt
    33001 cggttacacg ttctttggtt gcgccgccgc caacgtccag gaagttagcc ggttcgccgc
    33061 cgtgcagttt aactatgtcc atcgtaccca tcgccagacc tgcgccgtta accatacaac
    33121 cgatgttacc gtccagcgca acgtagttca gttcccactg tgcagcctgt gcttcacgcg
    33181 gatcttcctg cgactggtca cgcatttcgc gcagatcagg ctggcggaac agggcgttgc
    33241 catcagcgcc cagtttgccg tcgaggcaaa tcagatcgcc ctgtttggtg atgaccagcg
    33301 ggttgatttc gatcagcgcc aggtcgcgct ccaggaaaat ggtcgccagg cccatgaaga
    33361 ttttggtgaa ctgctgaacc agtttacctt ccagacccag tttgaacgcc agctcgcgtc
    33421 cctgatacgg catcgggcca gtcagcggat caagcgcaac tttatggatc aggtgcggag
    33481 tttcttccgc cactttttcg atttccacgc cgccttcggt ggaggccata aagaccacgc
    33541 gacgggaact acggtctaca acggcaccga gatacagctc tttagcgata tcggtcgccg
    33601 cttcaaccag aatctggtta accggttggc cattggcatc tgtttgatac gttaccagac
    33661 gcttgcccag ccagttttct gcaaaagcac ggatgtcttc tttgctgttt acaactttca
    33721 caccgcccgc tttaccgcgg ccaccagcgt gaacctgaca tttcactacc cacggaccgg
    33781 caccgatttt tgaagcggct tcttctgctt cgcgcggagt agtacaggca taacccaccg
    33841 gtgctggtaa gccatagcgg gcaaacagtt gttttgcctg atattcatgt aagttcatgt
    33901 gttctgtcca tccttcagta atcgttatct tttaaaccgt agaccgacac cctatccacg
    33961 tcggtctaca gtgcaggtga agcttaaact actacacgtc cagcagcaga cgcgtcggct
    34021 cttccagtaa ctctttgatc gttaccagga agcccacgga ttcgcgacca tcgatcagac
    34081 ggtgatcgta ggacagcgcc aggtacatca tcggcaggat ctcaacctga ccattcaccg
    34141 ccatcggacg atctttgata gcgtgcatac ccagaattgc gctctgcggc gggttgatga
    34201 tcggcgtaga catcagggaa ccgaacacac caccgttggt gatggtgaag ttaccaccgg
    34261 tcagatcttc aaccgtcagc ttgccatcac ggcctttgac tgccagctct ttgattttct
    34321 tctcgatgtc tgccatgccg agggtatcga catcacgcag aaccggcgtc accaggccgc
    34381 gcggcgtaga aaccgccatg ctgacgtcga aatagttgtg gtaaaccacg tcatcgccgt
    34441 cgatagacgc gttcacttcc gggtaacgtt tcagggcttc aaccaccgct ttcacgtaga
    34501 aggacataaa gcccagacgg atgccgtggc gtttttcaaa cgcttcaccg tactgcttac
    34561 gcagatccat aatcggcttc atgttgactt cgttgaacgt ggtcagcatg gcggtggagt
    34621 ttttcgcttc cagcagacgc tctgccacac gcttacgcag gcgagtcatc gggacacgtt
    34681 tttcactacg tgcagccaga gccggttgcg ccgccggagc agccgctgcc ggagcagact
    34741 ctttcgccgg ggctttcgcc agatgttttt ccacatcttc acgagtcaga cgaccaccca
    34801 caccggtgcc tttaatggcg ctggcgtcga gattgtgttc agccagcagg cgacggatcg
    34861 ccgggcttaa cgcatcgttg ttttgctctt ccagagacgc ctgctggcgt tgcgccggag
    34921 tggacgcttt ctcttcagat ttggcgctgg tttctttacc ggcgctgttg ccttcacgca
    34981 ggcgaccaag gatctgacga gacgttaccg ttgtaccttc atcttccaga accgcatcca
    35041 gaatgccgtc tgctgatgcc ggtacttcca gtaccacttt gtcagtttcg atttctacca
    35101 gcacttcatc acgtacgact gcgtcgccgg gttttttatg ccaggttgcg acggtggcat
    35161 cggctacgga ttcaggcagg tcagggacca gaatatctac gctactcatt gtgtatcctt
    35221 tatttattcg acgttcagcg cgtcattaac cagatcttgt tgctgtttct ggtgaacgga
    35281 catataccct accgccggag aggcggaggc cgggcggcct gcataacgca gagaagcccc
    35341 aaacggaatc acttcacgga aatgatgctg gctgcagtac catgcgccct ggttgagcgg
    35401 ctcttcctgg caccagacaa aatccttgac gtgagcaaac tgctgcaaca cttcctgcat
    35461 cgctttatgc gggaacgggt agagttgctc gatacgcaca atggcgacat cgtgttgatt
    35521 gttcttacga cgctgttcca gcaggtcgta ataaacctta ccagaacaca tcactacgcg
    35581 cttcacgccc ttcggatcaa gctcgtcgat ttcaccgatg gctggcagga aggtgccgtt
    35641 cgccagttct tcgaggctgg aaaccgccag cggatgacgc agcagggatt tcggcgacat
    35701 cacgaccagc ggacgacgca tcccgcgcag cgcctgacga cgcagcatgt ggtaaacctg
    35761 tgccggggta gacggtacgc aaacctgcat gttttgctca gcacaaagtt gcagataacg
    35821 ttccagacgc gcggaggagt gctccggccc ctgcccttcg taaccgtgcg gcagcaacat
    35881 caccagacca cacatccggc cccatttctg ctcgccagag gagatgaact ggtcgataac
    35941 cacctgcgca ccgttggcga agtcaccgaa ctgcgcttcc cagatggtca gggtgcgtgg
    36001 ttctgcggtg gcataaccat attcaaacgc cagcacggct tcttctgaca gtacggagtc
    36061 ccagacacgg aacgcgccct gaccgttatg gatgtgttgc agcggcgtgt aagtggaacc
    36121 gttagactgg ttgtggatca ccgcgtggcg gtggaagaag gtaccgcgac cggagtcttc
    36181 acccgacagg cgaaccggaa tgccttcatc aaccagcgtg gcgtaagcga ggttttccgc
    36241 accgccccag tcgaacagtt tctcaccggc tgccatcgcc tggcgatcgc cataaatctt
    36301 ggcaacgcga gactgcattt caactgcttc tggtaccgta ctgatacgtt tagccagttc
    36361 ctgcaggcgc ttcatctcaa ctttgttcgg gtactcttcg tcccattcgt ggttgaggta
    36421 cggcgaccag gtgaaagagt gcatgttcat cggacgccac tctgccacta cgcaatcgcc
    36481 agcatccagc gcatcgcggt acaggttaac catctcggtg gcatcttcca gcgtcgccac
    36541 tttttcctgc tccagcttgt cagcgtagat tttgcgcggt gtcggatgtt ttttgatttt
    36601 ctgatacatc agtggctggg ttgcgctcgg ctcgtcggct tcgttgtggc cgtgacggcg
    36661 gtagcatacc aggtcgatga agacatcacg tttaaaggtg ttacggaaat cgagcgccag
    36721 acgggtcaca aaggcaacgg cttccggatc atccgcgtta acgtggaaaa tcggtgcctg
    36781 aaccatctta ccgatatcag tacagtacgg tgtagaacgg gcatccagcg ggttagaggt
    36841 ggtgaaacca acctggttgt tgataacgat acgtaccgta ccgccaactt cataaccacg
    36901 cgctttcgac atgttcagag tttcctgaac cacgccctgc ccggtcactg cggcgtcacc
    36961 gtggatggtg attggcagca ctttgttgct gttcggctca tcaagtctgt ccagacgggc
    37021 acgaacagag ccgataacga ccgggcttac aatctcaagg tgagacgggt taaacgccag
    37081 cgccaggtgc accaggccgc catcggtctg gaagtcagac gagaagccca tgtggtattt
    37141 cacgtcgcct gtgccgaggt gttctttatg tttaccggcg aactcgtcga acaagtcttg
    37201 cggtttttta cccagcacgt tcaccagcac gttcagacga ccacggtgcg ccatcccgag
    37261 aaccacttcg cgggtgccgc tgttgccagc gtggcggatc atctctttaa gcatcgggat
    37321 taacgcgtca ccgccttcca gcgagaagcg ttttgcgcca gggaattttg cgccgaggta
    37381 acgctcaagg ccttcagcgg cggtcagttc gcttaagaag cgttttttct cttcgctatt
    37441 gaaagtcgcg cgaccagact cgatacgctg ttggatccag cgtttttctt cggtgctggt
    37501 gatgtgcata tactcggcac caatcgggcc gcagtaggtt tgcttgaggg cggaaataag
    37561 ctcgccgagt ttcatggttt ctttgccgct ggcaaatgaa ccgacgttga aggtttcctg
    37621 gaagtctgct tcggtcagat cgtggaaaga cggatccaga tcggccactt tatcttgctg
    37681 ccacagtccc agcggatcta gattcgcatg ctggtgacca cggaagcggt atgcgttaat
    37741 gagctgcagg actttaacct gcttcacatt ggtgtcaggg tcggagatcg ttgaagagta
    37801 acgtgaagcg tctttcgcca ggcggcggaa atattcacgc gtttgagagt ggaattgatc
    37861 cggtttgact cccgtaccag gtaactgctg gaacgtcgaa cgccagttag cgtcaaccga
    37921 gtcaggatcg gttaagaagt cttcatagag ctgttctatc cagctctggt ttgcgccaga
    37981 gaggtaagaa gagtccaacc aggctttcaa agcgctgttc tgcatcgtga tcccttaagc
    38041 atctttttta tgcttacttc gccgtggata ctaccacgca cagtgatgtg cgtgccgggg
    38101 ttcacttgcg agctcttgtc tacgtagtgg ctcgcgaagg aacctttaaa aactgtctta
    38161 gtgtcagggg cagtttttaa agatttcctg catttctttg ttacgcctga tgcgctgcgc
    38221 ttatcaggcc tgcgggtaat gacttgtagg ccggataagg cgttcacgcc gcatccggca
    38281 ctggttgcct gatgcaacgc ttgcgcgtct tatcaggcct acagtttacg cattacgttg
    38341 caacaacatc gacttgatat ggccgatggc gcgcgtcggg ttcagcccct tcggacatac
    38401 actgacgcag ttcatgatgc tgtgacagcg gaatacgctg aatgcatcgc tcaaaccgtc
    38461 gaggcggctg tcagtctcgg tatcacggct atcgatcagg aaacgatatg ccgctaacaa
    38521 gcctgccggg ccgataaact tatcgggatt ccaccagaaa gacggacaag aggttgaaca
    38581 acatgcgcag agaatacatt catacagccc gtcgagtttt tcgcgctgct ctggcatctg
    38641 taaatgctcg cgagctggcg gattttgtcc attattcaac aggtaaggct taattttctc
    38701 atattgcgca tagaattgtc ccatgtctac caccaaatcg cggatcaccg gtaaacctgg
    38761 cagcgggcga atcacaatct tcttgcccgg ctggttgagt gccgaaatcg gggtaataca
    38821 ggccagacca ttcttgccgt tcatgttcag accgtcggaa ccgcacacac cttcacggca
    38881 ggagcggcgg aacgacaggc tgggatcttt ctctttcagc tggataagcg catccagcag
    38941 catcatgtcg cgaccttctt ccgcttccag ggtgtaatcc tgcatacgcg gagcatcatc
    39001 aacatccggg ttatagcgat aaattgaaaa ctcgagtctc attttcctgt ctccgcatta
    39061 gtaagtacga atcttcggcg ggaatgccgg gcgcagtttc ggttccatgt tgacgcttcg
    39121 gcgcgtcatg gattccgact caggcagata cagggagtgg cacagccagt tttcatcatc
    39181 acgatccggg aagtcgaagc ggctatgtgc gccacggctt tcggtacgga agttggcaga
    39241 aacagccgtt gcatacgccg tttccatcag gttatccagt tccaggcact caacgcgctg
    39301 ggtgttgaac tcgctggaag tgtcatccag acgggcattt ttcagacgct cgcggatcac
    39361 tttcaactgc tcaagccctt tcgccatcgc atcaccttca cggaagaccg agaagttatg
    39421 ctgcatacat tcttgcagcg ctttacggat cgccaccgga tcttcaccgt tacgattatt
    39481 gttccaccgg ttcaggcgat ccagagacgc ttcaacatca gactcgctgg catcgtgcag
    39541 tgcgccctgc tcggcgatag actcttgcag atgcagacct gccgcgcgac caaagaccac
    39601 caggtccagc agcgagttgc cgcccagacg gttagcgccg tgtaccgata cacaagcgat
    39661 ttcaccaacg gcaaacagtc ccggaacaac cacatcttcg cctttctcat tcacagtcag
    39721 cgcctgaccg gtaactttgg tcggaatacc gcccatcatg tagtgacagg ttgggataac
    39781 cggaatcggc tctttcaccg gatcgacgtg agcgaaggta cgggaaagct ccaggatacc
    39841 cggcagacgg gattcgagaa cttctttacc caggtgatcg agtttcagtt tcgcgtgtgg
    39901 cccccacgga ccatcacagc cgcgaccttc acggatttca atcatgatgg aacgcgccac
    39961 cacatcacgg cccgccaggt ctttggcgtt cggcgcataa cgctccataa agcgttcgcc
    40021 atgtttgttc agcagataac cgccttcacc acggcaacct tcggtgacca gtacgcccgc
    40081 accggcaatg ccggtcgggt ggaattgcca catttccata tcctgcaccg gtacgcctgc
    40141 gcggatagcc atgccgacac cgtcgccagt gttaatgtgg gcgttagtgg tggactgata
    40201 aatacgccct gctccgccag tcgccagcac ggtagcgcgg gctttgaaat aaaccacttc
    40261 accggtttcg atgcacagtg cggtacaacc caccaccgcg ccatcctggt ttttcaccag
    40321 atccagcgca taccactcgg agaaaatggt ggtgtggttt ttcaggttct gctgataaag
    40381 cgtgtgcaac agtgcgtgac cggtacggtc agcagccgcc gcagtgcgtg ccgcctgctc
    40441 gccgccgaag tttttcgact gaccgccaaa cggacgttga tagatacgac catcatcgag
    40501 acgcgagaac ggcaggccca tgtgttcgag ttccagaatc gcttccggcc cggttttaca
    40561 catatattca atcgcgtcct ggtcaccgat atagtccgac ccttttacgg tgtcgtacat
    40621 atgccattcc cagttatctt catgggtatt acccagcgca acggtaatgc cgccttgcgc
    40681 agaaacggta tgggaacggg tcgggaagac tttagagagc agggcacagg tctggccgct
    40741 ctgggaaatt tgcagcgccg cgcgcatacc tgcgccaccg gcaccaatca caactgcatc
    40801 aaattctctg actggcaatt tcatcacaca ccccacacca caacgaatcc ataaatcacg
    40861 taaaccacca gtgcaacgac aatcaccagt tgcagcatca ggcgcaaggc cagcggttta
    40921 acgtagtcgg tcaacacctg ccacatgccg atccaggcat gaatcaagat agaaaacagc
    40981 gccagcaggg tgaacacttt ggtgaacgca gaggcgaaga aaccgatcca gacttcatat
    41041 gtcagttcgc cactggtggc gaaaaaaccg accatataaa tgatgtagag cgtcaggacg
    41101 atagcggtag cgcgaacgag gatgaaatca tgtacgccat tgcgtcctaa tgcggaggcg
    41161 ttgcttacca tacgaggact cctgcgagaa gtgaaagcac gacagtaata acaaaggaga
    41221 ttttggcgga gcgtttaccc gcttcgaatg tttcttccag atagccaaaa tccatcatca
    41281 tgtggcgaat acctacgacg acgtgatacg ccagagcggt aaggatgccc cacatgataa
    41341 atttgacgaa gaagctgccc ataatcgcgg aagcttgctc gaaaccttca ggggaagaga
    41401 ggctggtacc cagaagccac tgcaggatgc ccactgcaac aaaggtgatc acaccggaaa
    41461 cgcgatggag aatggacgct atcgccgtga tggggaaccg gatggtctgt aggtccagat
    41521 taacaggtct ttgttttttc acatttctta tcatgaataa cgcccacatg ctgttcttat
    41581 tattccctgg ggactacggg cacagaggtt aactttctgt tacctggaga cgtcgggatt
    41641 tccttcctcc ggtctgcttg cgggtcagac agcgtccttt ccataactgc gcgtcatgca
    41701 aaacactgct tccagatgcg aaaacgacac gttacaacgc tgggtggctc gggattgcag
    41761 ggtgttccgg agacctggcg gcagtatagg ctgttcacaa aatcattaca attaacctac
    41821 atatagtttg tcgggtttta tcctgaacag tgatccaggt cacgataaca acatttattt
    41881 aatttttaat catctaattt gacaatcatt caacaaagtt gttacaaaca ttaccaggaa
    41941 aagcatataa tgcgtaaaag ttatgaagtc ggtatttcac ctaagattaa cttatgtaac
    42001 agtgtggaag tattgaccaa ttcattcggg acagttatta gtggtagaca agtttaataa
    42061 ttcggattgc taagtacttg attcgccatt tattcgtcat caatggattc tttacctgca
    42121 agcgcccaga gctctgtacc caggttttcc cctctttcac agagcggcga gccaaataac
    42181 aaacgggtaa agccaggttg atgtgcgaag gcaaatttaa gttccggcag tcttacgcaa
    42241 taaggcgcta aggagacctt aaatggctga tacaaaagca aaactcaacc tcaacgggga
    42301 cacagctgtt gaactggatg tgctgaaagg cacgctgggt caagatgtta ttgatatccg
    42361 tactctcggt tcaaaaggtg tgttcacctt tgacccaggc ttcacttcaa ccgcatcctg
    42421 cgaatctaaa attactttta ttgatggtga tgaaggtatt ttgctgcacc gcggtttccc
    42481 gatcgatcag ctggcgaccg attctaacta cctagaagtt tgttacatcc tgctgaatgg
    42541 tgaaaaaccg actcaggaac agtatgacga atttaaaact acggtgaccc gtcataccat
    42601 gatccacgag cagattaccc gtctgttcca cgctttccgt cgcgactcac atccaatggc
    42661 agtcatgtgt ggtattaccg gcgcgctggc ggcgttctat cacgactcgc tggatgttaa
    42721 caatcctcgt catcgtgaaa ttgccgcgtt ccgcctgctg tcgaaaatgc cgaccatggc
    42781 cgcgatgtgt tacaagtatt ccattggtca gccatttgtt tatccgcgca acgatctctc
    42841 ctatgccggt aacttcctga atatgatgtt ctccacgccg tgcgaaccgt atgaagttaa
    42901 tccgattctg gaacgtgcta tggaccgtat tctgatcctg cacgctgacc atgaacagaa
    42961 cgcctctacc tccaccgtgc gtaccgctgg ctcttcgggg gcgaacccgt ttgcctgtat
    43021 cgcagcaggt attgcttcac tgtggggacc tgcgcacggt ggtgctaacg aagcggcgct
    43081 gaaaatgctg gaagaaatta gctccgttaa acacattccg gaatttgttc gtcgtgcgaa
    43141 agataaaaat gattctttcc gcctgatggg cttcggtcac cgcgtgtaca aaaattacga
    43201 cccgcgcgcc accgtaatgc gtgaaacctg ccatgaagtt ctgaaagagc tgggcaccaa
    43261 agatgacctg ctggaagtgg ctatggagct ggaaaacatc gcgctgaacg acccgtactt
    43321 tatcgagaag aaactgtacc cgaacgtcga tttctactct ggtatcatcc tgaaagcgat
    43381 gggtattccg tcttccatgt tcaccgtcat tttcgcaatg gcacgtaccg ttggctggat
    43441 cgcccactgg agcgaaatgc acagttacgg tatgaagatt gcccgtccgc gtcagctgta
    43501 tacaggatat gaaaaacgcg actttaaaag cgatatcaag cgttaatggt tgattgctaa
    43561 gttgtaaata ttttaacccg ccgttcaaat ggcgggttaa tttttatatg cctaaacaca
    43621 aaaaattgta aaaataaaat ccactatcgg acattcatat attctaaaaa aaaatacatc
    43681 ggtataaatt ataaacaaat agatgatttt taatcatcta tcttcctctc agttacatga
    43741 ataaaccacc aaatatagcg cacccctata attcccaata ctttattcat caaacattta
    43801 attcatacca tcaattaatt acttttacat ttaataaaat taataatatt tttaagaaaa
    43861 actaaaaaca ttcgacaatc aaataagata ttcttgcacc gatatacttt tttcttttct
    43921 gttaaaggac tcagttatgt ttaaaggaca aaaaacattg gccgcactgg ccgtatctct
    43981 gctgttcaca gcacctgttt atgcggctga tgaaggatcc ggtgaaattc actttaaagg
    44041 tgaagttatt gaagcaccgt gtgaaataca tcaggatgat attgataaag aggttgaact
    44101 cggtcaggtg accaccagcc acattaatca gtcacatcac agcgatgccg ttgctgtcga
    44161 cctgctctta gtcaactgtg atctggaaaa ctccagcaac ggttccggtg gcaagatttc
    44221 taaagttgca gtcacctttg atagctcagc gaaaaccacc ggcgcagatc cgattctcaa
    44281 caacaccagc acgggtgaag ccaccggcgt cggcgtacgt ttaatgaata aagatcaaag
    44341 caatatcgtt ttaggcaccg ctactccaga tatcgacctg gctccgacct ccagcgaaca
    44401 aacgctgaat ttctttgcct ggatggaaca aattgatcag gctacacctg taacgccagg
    44461 cgcagttacg gcgaatgcaa cctacgtgct ggattataaa taagtattac tttggcggcg
    44521 ctatcgccgc ctttcataaa atatttatca ggtatggaca ccgtgaatat ttatcgactc
    44581 tcttttgtat cctgcctggt cgtggcgatg ccttgcgcat tggcggtcga attcaatctg
    44641 aatgttctcg ataaatcaat gcgcgaccgc attgatattt cattattaaa ggaaaaagga
    44701 gtcattgctc ccggtgagta ttttgttagc gttgcggtaa ataataacca aatcagtaac
    44761 gagcaaaaga ttaactggca caaaaatgac gataaaacca ttccgtgcat caatgattta
    44821 ctggtcgata aatttggctt aaaacctgaa gtccgtcagt cgttaccatt gataaatcag
    44881 tgcgtcgatt ttagctcccg acctgaaatg ctcttcaatt tcgatcaagc caatcagcaa
    44941 ctgaatatta gtattccgca agcctggctg gcgtggcact cagaaaactg gaccccaccc
    45001 tccacatgga aagaaggtgt cgccggtgtc ctgatggatt acaacttgtt tgccagcagc
    45061 taccgcccac aggatggcag cagcagcact aatcttaacg cctatggaac agccggaatc
    45121 aacacggggg catggcgctt acgcagtgat tatcagttga atcatactga tagcgatgat
    45181 aaccatgaac agtcaggcga aatatcacgt acctatcttt ttcgcccgtt accgcaatta
    45241 ggttcaaaat taaccctcgg cgaaacggat tttagtccca atattttcga cggtttttct
    45301 tataccggcg cggcactggc aagtgacgat cgaatgttgc catgggaact acgcggctac
    45361 gccccacaaa ttagcggtat tgcacagacc aatgccacgg tgacgatcag tcaatcaggc
    45421 cgcgtcattt accagaaaaa agtcccacca ggcccattta tcattgacga ccttaatcag
    45481 tctgttcagg gcacactgga tgtcaaagtg acggaagaag atggtcgggt gaacaatttc
    45541 caggtttcag cagcatcgac gcccttcctg actcgtcagg gacaggttcg ctataaactg
    45601 gccgcgggtc agccacggcc ctccatgtca catcaaactg aaaatgaaac cttttttagc
    45661 aatgaagttt cctgggggat gctgtcaaac acctcgctgt acagcggcct gctgctttct
    45721 ggtgatgact accattctgc cgcaatgggt attgggcaaa atatgctgtg gcttggtgcg
    45781 ctgtcgtttg atgtcacgtg ggccagtagc cattttgata ctcagcagga cgagcggggc
    45841 ttaagctacc gttttaatta cagcaaacaa gtggatgcca ctaacagcac gatttccctc
    45901 gccgcttatc gtttctccga tcgtcatttt cacagctacg ccaactatct ggatcacaaa
    45961 tacaacgaca gcgatgcgca ggacgaaaaa cagacgatca gcttatctgt gggccaaccg
    46021 attaccccac taaacctcaa tctttacgcc aacctgctac atcaaacctg gtggaatgca
    46081 gacgcctcca cgaccgccaa catcacggca ggttttaatg ttgatattgg tgactggaga
    46141 gatatctcta tttctacgtc attcaataca acccattacg aagataaaga tcgcgacaac
    46201 cagatttacc tgtcgatttc gctccccttc ggtaacggtg gtcgggttgg ctatgacatg
    46261 caaaacagta gccacagcac cacacaccgc atgtcgtgga acgatacgct ggatgaacgt
    46321 aatagctggg gcatgtctgc cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg
    46381 agcggtaact atcagcacct gagttcagcg ggtgagtggg atatttctgg tacctatgcc
    46441 gccaatgatt acagttccgt cagcagcagc tggagcggtt ctttcaccgc aacccaatat
    46501 ggtgcagcgt ttcatcgccg cagctccacc aatgaaccac gcctgatggt cagcaccgat
    46561 ggcgtggcag atattccggt tcagggcaat ctcgactaca ccaaccattt tggcattgcg
    46621 gtggtgccgt tgatttccag ttaccagcct tccaccgtgg cggtgaacat gaatgactta
    46681 cccgacggcg taacagttgc agaaaacgtt atcaaagaaa cgtggattga aggcgcgata
    46741 ggttacaaat cactggcttc ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc
    46801 agcggtcagt ttcctcccct cggagcggat atccgccagg atgacagcgg cattagcgtg
    46861 gggatggttg gcgaggaagg acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt
    46921 accgtggtct ggggtgatag ccagcattgc tcgctccatc ttcctgaaca tatggaagac
    46981 accgcaaatc gcctgatttt accttgtcat taattaagga aatgaaaatg acatttatga
    47041 aaggactacc ccttttgttg ttggttgcca gtctgtgcag ccacgctgca ctacaacccg
    47101 atcgcactcg tattgtgttt aacgctaatg ataaagctac cagtctgcgg gtggataatc
    47161 gtagcgataa actcccctac ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa
    47221 gcgatgatct tctggtcgcc ctgccgccga tacaacgtct ggaaccgaaa gcaacaacac
    47281 aggtacggat agtaaagcaa gcctcgacca caaaattacc gggcgatcgc gaaacgctat
    47341 ttttctacaa tatgcgtgaa attcctcccg ccccggaaaa aaacagcgac catgccgttc
    47401 tgcaagtcgc cattcagagt cgtatcaagg tgttctggcg accggctgca ttacgcaaga
    47461 aagcaggaga aaaggtcgaa ctgcagttac aggtcagcca gcagggcaac caactgacac
    47521 tgaaaaatcc taccgcgtat tatctgacca ttgcctacct gggacgaaat gaaaaaggcg
    47581 ttctccccgg cttcaaaacc gtgatggttg cgccttttag tacggtcaac acaaacaccg
    47641 gaaattatag cggcagccag ttttatctcg gttatatgga tgattacggc gcgttacgca
    47701 tgacgacgct caactgcagc ggacaatgcc atttacaagc cgtggaggcg aagaaatgag
    47761 tgctggcaag ggagtgttgc tcgtcatctg tctgttattt ctgtcgttga agtctgcgct
    47821 tgccctgaac tgctactttg gttcatccgg tggctcggta gaaaaatcag aagcgattca
    47881 accttttgct gtaccaggca atgccaaact cggcgataag atctgggaat ctgacgatat
    47941 taaaataaga tctgggaatc tgacgatatt aaaattcccg tctattgtga caacaatacc
    48001 aacggcaact ttgaaagcga acatgtctac gtatgggtaa acccctaccc gggggtgcag
    48061 gatcgttatt atcaactcgg agtaacttac aatggcgttg attatgacgc caaccaggga
    48121 aaaagccgca tcgacactaa ccagtgtatc gacagtaaga acattgacat ttatacccct
    48181 gatgggatgg cagaacaaaa tttgctcggg tgatcccgct aatattcaca tgtcgcgaac
    48241 ctttctcgct cgcatgcggt tatacgtcaa aatacgagaa atgccgccgc atgattatca
    48301 aagtacgctt agcgactata tcgtcgtgca atttgacggt gccggtagcg ttaacgaaga
    48361 ccctactgcc caaaacctga aatatcatat tactggtctg gaaaacattc gcgtgctgga
    48421 ttgcagcgtc aatttttcca tttcaccgga aacacagatg attgattttg gtaaatttaa
    48481 tttgctggat attcgtcgcc acacaatgtc gaaaacattc agtattaaga cgacaaaatc
    48541 acaaaatgac caatgcaccg acggatttaa agtcagttcc tccttttata ccgaagaaac
    48601 gttggttgaa gaagataaag cattgttgat tggtaatggt ctgaagctac gtttgctgga
    48661 tgaaaacgcc tcgccttata ccttcaataa atatgctgaa tacgccgatt tcaccagcga
    48721 catgttggtc tacgaaaaaa cctatacagc tgaactttcg tccattgcag gtacccccat
    48781 cgaggctggg cccttcgata cagtggtgct ttttaaaatt aactataact gatttgccat
    48841 gatgcccttt gcagccggaa agggcatctc tttcagaaaa ttgctgattt tccccttttc
    48901 cttgattgct gttatttttt cctagcccgg ctttctttat gctgtatcga tacaccaaag
    48961 agaaaataat gagggagcga aggatgccag ttttgcagtg gggaatgtta tgtgtgctat
    49021 cacttctcct ttctattggt ttcctcgcgg tacatctccc ggcggcgcta ttacttgggc
    49081 cgatgatcgc cgggatcatc ttcagtatgc gcggaattac tctgcaactt ccccgctccg
    49141 cttttctcgc cgcgcaggcc attcttggct gcatgattgc gcaaaacctc actggttcga
    49201 ttctcaccac gctggccgcc aactggccga tcgtgcttgc tattttactg gtgacgctgc
    49261 tttccagcgc catcgtgggt tggttattgg tgcgctatag ctcactgcct ggaaataccg
    49321 gtgcctgggg ttcttctcca ggcggcgcag cggcaatggt cgctatggcg caagattacg
    49381 gcgcagatat tcgcctggtg gcgtttatgc aatatctgcg agtgctgttc gtcgcggggg
    49441 ccgctgttct ggtcacccgc atgatgctgg gtgataacgc tgaagcggtt aaccagcaaa
    49501 tcgtctggtt cccgccagtg agcattaatc tcctgcttac gattttgctc gcagtcgttg
    49561 ccggtacggc gggatgcatg ctgcgtcttc cttccggcac gatgctcatc ccgatgctgg
    49621 cgggcgcagt gctccagtct ggtcagctca tcaccatcga actaccggaa tggctgctgg
    49681 cgatggcgta tatggcaatt ggctggcgga ttggtcttgg tttcgataaa caaatcttac
    49741 tgcgggcact acgcccgcta ccgcaaatcc tgctgtcgat ttttgctctg ctggctattt
    49801 gtgcaggtat ggcgtgggga ctgacccggt ttatgcatat tgattttatg actgcctacc
    49861 tcgccaccag tcccggcggg cttgataccg tggcggtcat cgccgcaggg agcaatgccg
    49921 atatggcgct tattatggcg atgcaaaccc tgcgcctgtt cagtattttg ctgacggggc
    49981 cagccattgc gcggtttatt tcaacctatg cgccgaagcg gtcggcctag tgctggcagc
    50041 ctggacacca gtaaaacggg cgcgaggaca acgtggtttt ctcaatgatg ccgccacagc
    50101 gttcgcacag ttcgccatca cgatgaaaaa ccttaaagcg aaacagcgcc ccatgatgct
    50161 tattctcatc cacctgcccc cgcgtagcgt aagaaaaccg aggaatctcc agtaacgcgt
    50221 gtgcgagtgc atccagttgc gccgcattga gatctttcgc tttatgattt ccagtcaacc
    50281 caacctgcca gaggatctcc acccgcaaat aattgccaag cccagccaga aacgcctgat
    50341 cgagcagtaa tcctgcaaac tgacggttac gaaagcgcgg cgacaataac cgttccttta
    50401 ccacctccgg cgtcagattc ggatccagca catcgggacc aacgcgttgt aaaaacggat
    50461 gcgtggtcag ttgttccggc ctcaacatct caatatccga ggcgctataa agcagaatgg
    50521 ttttgtcagc cgtttgcagt tttacccgca atactcgcgt ggtctgcggc tcttcgccgg
    50581 tatcaaccac gcgccagacg ccgtaaagct gattatggct gtagagcgtt aaaccgttgg
    50641 gaaaatgagt taacaacgcc ttaccacgcg tttccacatg ggtaacgtgt tgaccgataa
    50701 gttgtgattg ataagttttt aactgcggga aggcaaacca gacatcagtt agtggtttgc
    50761 ctttgatcgc cgcctccagg ttatccgctg cacggcggat ctccgggcct tcaggcatgg
    50821 tgatatcctt ttttcattct ttattaagcg gggttatgct gcgacaacaa tccccttttt
    50881 ggcaaatgta gctcgtaatc ggcgggcgaa cgccagagcg tgctcgccgt cgccatgcag
    50941 gcagaccgtt tgcgccgcga ccgttgccca ttcgccggtg atacttttga ctctgccgtg
    51001 ttgcaccatc tccagcgttt gcgccaacgc ctgttcttcg ttttcaatca acgcgcctgg
    51061 ctgacttcgc ggcaccagcg agccgtcagc ctgataaccg cgatcggcaa acacttcctc
    51121 gcgcgttgtc aggccatatt gcttgcctgc acgaatcagc tcgcttcctg ccagcccaac
    51181 gagaaccaat gccggatcgc aagcgtatac cgctctggcg atggcgtctg ccagttgtgc
    51241 ttctttcgcc gcctggttgt acaacatgcc gtgcggtttg acatgacgca ttacgccgcc
    51301 ttgcgcacgg gcaatcgttg ccagcgcgcc aatttgatac agcgtctggg cgtaaacggt
    51361 ttccggcggc agctgcatgg cgctgcgacc aaaattttcc ctgtcgggaa aactcgggtg
    51421 agcgccaatc gcgacaccat tttttattgc ttcacgcacg caagcctgca tggtttgcgc
    51481 atcgcctgca tgaaatccac aagcaatatt ggcagaggaa accagcgtta atagctctgc
    51541 gtcgctggcg cagccttcgc ccagatcggc gttcaggtca attttcattg tgcagccgcc
    51601 acgctaattg ttcaaaataa cgttgctgat cttgccgcgc tttcagtgcc tcttccagtg
    51661 aacactggac aaaatgaatc ggctgaccga gcggaatttg cgccagatgg tacatatcag
    51721 cctcaatgat acaggcaata cgtggataac caccggtggt ctgtgcgtcg ttcatcaaca
    51781 caatcggctg cccgttatgc ggcacctgca ccacgcccgg taacaaaccg tgagataaca
    51841 gttcgcgatc ggtgatgcgt tttaaaattt gcccctgtaa acgatagccc attcggttac
    51901 tttgcgagct aagctgccag ggcgaacgcc agaatgcatc ctgcgaggcg cgatcgaact
    51961 catgatattc cggccccggc aaggcgcgaa tgcggttgcc ccacagcagc tgtttaacgc
    52021 cctgcgcttc cataaaatca cgcttcgatt tgccaatcgg gagtcagtca ccatccttca
    52081 gtaaacggcc ttccagcccg ccaatcccca ctttgagatc ggtgctgcat gaccccatta
    52141 ccggcggaac atcaatacca cccgcgaccg ccagataact gcgcatcccg tgctgcgggc
    52201 gtttaagcgt taaacgctgg cctgctttca tcggcaatcg ccagccggtc cagacggcgt
    52261 tatcatccaa ccgcgcttcg caacctgcac ccgtcagggc aaaccaccca tcagtttcga
    52321 actcaactgt taactgaccg agcgtgatct ccagcgcggg ggcattggcg tcattaccca
    52381 ccagcaggtt agcaatgcgc agcgcaggca tatccagtgc gccacagtgg ctgatacccg
    52441 actggcaaaa accgtgacga ccgccatcct gcacagtggt gtacatgccc gcacgaataa
    52501 tcttcagcat attccctcct tctgcggtac aaatcgcacg ctgtctcccg gacgtaataa
    52561 gatgggttcg tcacgcgccg gatcaaacag gctgagtgag gtatgaccaa tcaactgcca
    52621 gccacccggc gttgccagcg gataaacacc agtctgcggc ccgccgatcc cgacagaacc
    52681 tgccggaacg agcaagcgcg gttcagcgcg ccgtggcgtg tgtagttgtt ccggcaaact
    52741 cccgagatac gggaagcccg gttgaaaacc taaaaaccag accacgtatt ccacggagga
    52801 gtgcaattca acaacctgtt tttcgctcaa cccgcaatac gccgcgacca ccgccaaatc
    52861 cggtccgcct gcaccaccgt aaaccaccgg aatttcaata aagcgagact caggttccag
    52921 ggcctcgctc tcctcccacc agcgttgcaa acgctctatg gcatccagcg ccagcgactc
    52981 aggattacgc aaaatcaccg tgatattgtt catgccggga atggcttcaa ccacattcgg
    53041 catatccacc agacgctgcg ccagtcgcca gatccgtttc tggctagcca gcgtcaccgg
    53101 cggttccagt tccagcacta ccgccgtttc acctatcaga taacaacgcg ctcgttgcac
    53161 tttcgatccc tctgattatt cgttatgcag gattaggaat atcaataaag gtcacatcaa
    53221 gatcggtatt ttcattcagc cactcgctca atgcgcgaat accaccgcgt tcagtggcat
    53281 ggtgacctgc agcataaaaa tgcaatccct gctcgcgggc tgaatgaatg gtctgttcag
    53341 aaacttcgcc agtaataaaa gcatccacgc caaaacgcgc ggcgctatcg ataaaacttt
    53401 gcccgccgcc cgtgcaccag gcgacgcgct ggaccacctc aggtccggta tcgccacacc
    53461 ataacggctt acgtcccaga cgcgcttcaa tccaggaggc tagttccatt cccggcacag
    53521 gcatggtcag ctctccccac ggcaccaacg gctcaatttc gcccatgacc gtgatcccca
    53581 gtaacgccgc cagctgtgcg ttattgccca gctcaggatg tgcgtcaagc ggcaaatgcc
    53641 agccatacag gttgatatca ttcgccagca acgtttttaa acggttacgc ttcattccgc
    53701 gaatgaccgg agactctcct ttccagaagt agccatgatg cacaatgacc gcatccgcgc
    53761 ccagacgcac tgcctcatcg agcaaagcct ggctggcggt gacaccggta acaatttttt
    53821 gcaccgtctc tttgccttcc acctgcaaac cattcggcgc gtaatcacta atcgccgcgc
    53881 tgttcagttt ttcgttaatc agttgttcca gttcggtgtt tttcatcttc gcgccttccg
    53941 tattctgata gctacaccgt taacatagcg ttacatcgcc tgttcgtcga tagccaatct
    54001 tttgacatat taagaaacaa acccgacgcc aaaagacgca aattgtttat atcatcacgc
    54061 ttcagctaat gtatatttat gcaatcagac agcatattgc ataaataatc agatctgtat
    54121 caaagtttta actttaagga aacacaagca cgccgcgcaa ctattacgtg acgttgccgt
    54181 caattgtgga attattatga ataaacacgc atcacaaccg cgcgctattt actatgtcgt
    54241 tgcgctgcaa atctgggaat attttagctt ttacggcatg cgtgccctgc tgattctcta
    54301 tctcaccaat caactaaaat acaacgatac tcacgcctac gagttattta gcgcctactg
    54361 ttcgctggtg tatgtcacgc caatcctcgg tggctttttg gcggataaag ttctcggcaa
    54421 tcgcatggcg gtgatgctgg gggcgttgtt gatggcgatc ggtcatgtgg tgctgggtgc
    54481 cagtgagatc catccgtcat tcctctatct gtccctggcg attatcgtct gcggctatgg
    54541 cctgtttaaa tctaacgtaa gctgtctgct cggcgagctg tatgagccaa ccgatccgcg
    54601 tcgtgatggc ggtttctcgc tgatgtatgc ggcgggtaac gtggggtcta ttatcgcacc
    54661 tatcgcctgt ggttacgccc aggaagagta cagctgggcg atgggctttg gcctggcggc
    54721 ggttggaatg atcgcgggtc tggtcatttt cttatgtggc aatcgtcatt tcactcatac
    54781 ccgcggcgtt aacaaaaaag tactgcgtgc gacaaacttt ctcctgccga actggggatg
    54841 gctgctggtt ctgctggtgg caacgcctgc actgattacc gtgctgttct ggaaagagtg
    54901 gtcggtatac gccttaattg tcgcgactat tattggcctg ggtgtactgg caaaaattta
    54961 tcgcaaagca gaaaaccaga aacagcggaa ggagctgggg ctgattgtga cgctcacctt
    55021 cttcagtatg ttgttctggg ccttcgcaca acagggcggt agctcgatta gcctttatat
    55081 cgaccgcttc gttaaccgcg atatgtttgg ttataccgtt ccgaccgcga tgttccagtc
    55141 gattaatgcc ttcgcagtta tgctgtgcgg tgtgttcctg gcgtgggtgg ttaaagaaag
    55201 cgtcgcaggt aatcgtaccg tgcgcatctg gggaaaattt gctcttggcc ttggcctgat
    55261 gagcgccgga ttctgcattc tgaccttaag cgcccgctgg tccgcaatgt atggtcactc
    55321 ttctctgcca ctgatggtgt taggcctcgc ggtgatgggc tttgcggaac tgtttatcga
    55381 cccggttgcc atgtcgcaaa ttacgcgcat tgaaattccg ggcgtgaccg gcgtattaac
    55441 cggcatctac atgctgcttt ctggcgcgat cgcgaactat ctggcgggcg tgattgccga
    55501 tcagacatcg caggcttcgt ttgatgcttc cggggcgatc aactactcca tcaatgcata
    55561 tattgaagta tttgatcaaa ttacctgggg cgcactggcg tgtgtaggac tggtactgat
    55621 gatttggctg tatcaggcgc tgaaattcag aaaccgcgcg ctggcgctgg agtcataata
    55681 ggccagcacc gatgcctgat gcgacgcttg acgcgtctta tcaggcctac gtatttcctg
    55741 caatttattg aatttgcaca aatttgtagg ctggattagg cgttcacgcc gcatccggca
    55801 tctgggctcg attgcctgac gcgtctgtta tttccccttc cgcgccgcct catacgctgc
    55861 caacgtttgt actctcgcct ctttgtgctc gactatcggt tgcggataat ccagcttcac
    55921 acctgctttc tgcgcccact tccacggctc atgcaccgct ttccctggta catcgcgcag
    55981 ctccggtaac catcgacgaa taaactcgcc ctcacggtca aatttctcgc cctgagttat
    56041 cggattgaaa atacgaaaat acggcgcagc atcagtaccc gtagaagccg cccactgcca
    56101 gccaccgtta ttagctgcca aatcaccatc aatcagctgc gacatgaaat atcgctcgcc
    56161 ttcgcgccag tcgatcaaca aatctttcac cagaaaactg gctgtaatca tccgtagccg
    56221 gttatgcatc cagccagtgc tgttaagctg acgcatagcg gcatcaataa tcgggtatcc
    56281 cgttttgcct ttctgccagg cctgtaaatg tgcgggattg ctttgccact gcacacgatc
    56341 cgtccaggca ataaatggac gatgtttaca caacgagggg taatacgtca tcagatgacg
    56401 gtagaactcg cgccagatca gctcactaag ccagacacta ccggccccac cgtccagcgc
    56461 ctgcggctgt tccgccaaca agcgatgcaa acactggcga ggcgataacc cgcccgttgc
    56521 caagctggcg gacaaacggc tggtgccttc cactgccgga aaatctcgtt gttgctcata
    56581 ctctccagca ccgttctcgc aaaactggcg taattgcgca atcgccgctt tttcttccac
    56641 cgggaaatgc gcagtatcga aagactgacg aggataattc agcgtaatgg atggcgcggg
    56701 ctttatcgat ccgctactac gaacttttgg tgcagcgacg cactccggca tcccttcccg
    56761 cagccgtttc agccaggcat tcttaaaagg cgtaaagact ttgtacatct cgtgattgcc
    56821 ggtcatcacc gcccccggcg gcaggatcac gctgtcatca aatccttcac acaccacgtt
    56881 acgcagcgct ctttcaacgt ccacatcccg cgctcgctca ttcacctcat actgatagtt
    56941 ataaaaaaga tgagtaacgc tgttttccgc gcacacctgt ttaactattt cgacactggc
    57001 gacaaagtca tccacttcac ggaacaataa aggaatacct ttttccgcca gcgcgatttg
    57061 tagcccattc agttgagcat tgataagttc agcctgacgc ggcgacatgt tatgcgtctc
    57121 ccacttgcgt ggtgtagcga tatacagcgc cagcacgcgt gcagacgaat tgcggcaggc
    57181 ggcagccagt gcgagattat cgtgcagacg taaatcctgg cgaaaccagg ccagatgggt
    57241 agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg gccaaagttc gaaataacgc
    57301 tgccctgaaa gccaggcgtc aggataaagc gccatatagt gtttgatacg catcagcggc
    57361 gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa gcgcagccag ctgctggcgt
    57421 tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt gcatcaaaac gttggtgtga
    57481 tcgcgcgggt tggcgggatg agaaagcaaa acaattacgc gttggcggta ctggttgtaa
    57541 aaatcatcca gattttgcca ctgatgaata tcagccacaa acgggccaag tttgcggtac
    57601 tcgggctgag aatgggccag aaagacgagt ttatagcggc tgtgataatc gagaagcgcc
    57661 ccgcgcgtta agccatgttc tttcagtcga tgtaactcat gaagggcgaa gatacggatt
    57721 agggtgctgt catcaaatcg ttgtagattc attactgctc ctgaacggta tacaggagta
    57781 agtgtaggcg ttggtaaatg atgtttgtga agttaatccg ctagcagggc aagattacga
    57841 aagcgcgctc cccgcaagca actaatcaag gtagttaatc atggctgaac acgatattac
    57901 gccagaagtc actatttcca aaacacagcg ccgctataaa gtcggctatg tcagagcccg
    57961 ccaccaggac cgcaaaaccg gtatagcccg gtattattca caacatccca gtctgcacct
    58021 taagggcgac tggctgaaag aggccggatt tgataccgga cggggtgtca cggtgaagat
    58081 ttcggagggg ggcctgacca tcatcgctga cagtgacgag gtgcaggaac tgcgggatgg
    58141 gatgtttagt gtgctgaacg aaagttaaat attaaaaaag ctgggaagat ttaaaacctt
    58201 cctagctttt tattctgtct tattataatg gatactcatc atcttccgat gaatttataa
    58261 attgctccaa gtttacatga agagtactta acctagtttt taactcatta agtgaagcga
    58321 taatttcata ttttccaaca taatcatatg atggatcgta ttcattatct atgtcagaag
    58381 ctaaaaatga tgatattcgt agtttaaatt tagcttcatc tttattacat tcattggcat
    58441 aaggtgaatg ccctatcata gcatattcat ctaatctaaa aggataagat ggttttaatt
    58501 catcgatcaa tgaaatttca atccatgttc tatatccagg cattaatgga ctaatttctt
    58561 tgagcaaaac ctttccactt aataatgtat tgagattgtt cattattatg tctcccacgg
    58621 gaaaaatata tgtctaacac ttccaccatg ttgatgaact tgtaaatgtg gtaaatcacc
    58681 atcaggattt cctggcccat gacctgacac tctatatgaa ggttttgtta cagaaggatc
    58741 ttttatatca tcccaatgat atgaacctgc ttttgtttta ttttcagtaa gatattccat
    58801 taagttctta gtgctggggc ctgactcacc tgtcatattt ttataattat gacctaaata
    58861 tctatccata aacaattgtt cagcgtcact tctagaccta acagtaactt tggtttcact
    58921 actatctaat aaacgattcg ctttttggtt actaacagca catggagtta atccaagtgg
    58981 atcagcatat tttagaggat taggtgcata actgtagaga tttaaaccgc ctctcaaccc
    59041 tatcgggtca gttaccatga accgcccgac atccggatca tagtacctgt gcagattgta
    59101 gtgcagcccc gtttccctgt cgaggtactg tccctggtag cgcaggttct gctccacccc
    59161 gccgtggtcc ttctcctgta tcgtgttacc ccagacctga taccccgtct cccacacgat
    59221 tttcccgtcg ctgtccgtca tctcttccgg cgcgccgttc acatccgtgt gaaaatacag
    59281 tatccggttc gggccctctt tgcccgcctt atccacccgc gccagcggtt cataactccc
    59341 ctggtccctg tagatgtaca ggctgcctgt tccctccggc ctgctctcct gcgccagccg
    59401 cattccgtcc cataaaaatt ttgtccggtt gtacggctta ccgtcagggc tgatgtgctg
    59461 tttttccgtc cgcctcccca gtgcatcgta cacataccgg tatgtccccc gcgctgtcgt
    59521 cacttccacc atccggtgtt ccgcatcata acggtaactc tgcgttccgc tccggccctc
    59581 cttttccacc gcacggccaa attcgtcata acggtagtgc acaccacggt aagacatcag
    59641 ccggttgaac ggcaccacgc tgttttctgc cgtggccctt tcccccgcgc gcctccccag
    59701 caggtttccc gccgcgtcca cctccgtttc cggctggtgc cacaccgggt gccactgcgc
    59761 ctgctccacc cgccccagcg ggtcgtgcgt ctccgtgata tgcccctgca ggtcatacac
    59821 atagcgccat ttgccgccct gagggtcggt catccccagc aacagtcgtt cttcgtcgct
    59881 ccagcggaat gtcgtcacct gtccggcttc gtcctggtac gccgtcacct gattctgcgc
    59941 atcccactgg tgataacgtg tgcgccccag tccgtccgtc acgcacagcc tgcgctgcgc
    60001 caggtcccac gccagccggt actgttcacc gctgtccgtg tggtgccccg tcacccgcca
    60061 tgccgcgtca tccggacctt cataccagcc ccactggtaa taataccctt cgccgcccgg
    60121 ctggcggtgc agcgtcatgc agtggttttc gtcgtaagca aactcacgca gcaggtgccc
    60181 cgtgctgtcg tacacgccgt tcaggtggcc ggcctcgtca tagctgtaac gacgcagcag
    60241 taaaggtgca gtctcaggga aatgctgata aatctgtgtc acccgccggg gatgcgccgc
    60301 cagttcgtaa tacagacgga tgcacggacg ctgctgttcg ccgctgattt ccacaatgcg
    60361 gccccggtcg tcgtagtaca gattcagaca gttgctgtta cggtcgccca gcattttcag
    60421 ccgctggcgc tgcgggtggt gcgggtcagc ctcaaacaga aaacactgcc cgtcatcgct
    60481 gctgataagc cagtgcccct gctcaccacg ccttaccgcc agcccttcac cggggctgta
    60541 aaacccgccc cccggcagta cggcttccat atccaagcgt cgccccatgc ccgacacaaa
    60601 ggtcatgcag ttttcgtctg cgctacccgg tgtgcacacg aggctgactt catacattac
    60661 actccagccc tgaccaaaca ttccttccgt acgttcgtca cgactgctgt acacgcgctg
    60721 ccagtccagc ggaaagtggc ccggcagact gaaatcgaag tcatcgtcat cggccaggta
    60781 tttggccccg gtggcggcat tgaccggatg cggaagggca ctggtggcct tctgcgtcag
    60841 catgctcatc ccgacactgg cagcaaaaca ggcgatttta gaaagcattt tgcccgggcg
    60901 aagaaacatc agcgccacgg ttgccaccag ggttatctgc gacctgccgc ttctgatatc
    60961 cctgaccacc agtaacggac cgccaatgcg cacatccggt gacacaaacg ccccgttccc
    61021 cggctcattc accaccctgg cctcgcaggt acagcgtgcc ccgctgcgtg tcgccggctg
    61081 gctgttgata cgcacatccc gcgcgccttc cgcgatatat tccgctgcgg gcggctcgtc
    61141 atcgcctttg aacagattac tgatgctgcc aataaccgca ttcactccct gaaaggcggt
    61201 ttccagcata ccgcccggat tcagcaggtt aagggccgtt cccagggggg catccagaaa
    61261 ggcggctttt ttttgtgcga gaaactgcgt cagtgaggaa ggatgtttat ggcagtcgat
    61321 tttatcgtca ggggtcgggg tggatcccgg ggccgcactg gccacagtgg gctgccatag
    61381 ttccgacaca aaatgtgtca cgccatccac catttcagaa cgcccttctt ccgtactgag
    61441 ctggtcgtgc agagcccggg acatcagcac aggccctgtc accgggttaa ccgccagggc
    61501 tatggccccc gccatttcca gatattcccc ggccacatcc cggaacgttc tggcatcctc
    61561 ctgacgctgc gcttcttcct gttctgcctt cgcctgcgct tcctgcgcct ctatgtcctg
    61621 ccgcgatgcc gcagtcgccg ctgcccgcgc ggcaggtttg ctgttgatcc atgtgtcacc
    61681 agagccggtg aggatgtaac cttcaatctg aggcgggaaa agcatatttg cgagggctga
    61741 acaggcttcg ctgacctttt ctgtcacgcc ggtgtacacc atcgccacac ccgccacaaa
    61801 accggtcagc acgacggccg tgcaccccat cgtgatcccc actgacgtcg ccagtaacga
    61861 ggttgtgccg acaatgaggg catcaataac gacatttgcg gccacctcca gcaccgcccc
    61921 cagcacatct gccataaacg acgtatgcag cagggcatca ccctgtcttg ccgcccagaa
    61981 tgcctcttcc atgtcccgtt ccctgtatga ttaattcgtc ctgacttcct gccgcatcct
    62041 gaagcgcaga ttctttttaa tttcagccca gcgcgtcata tctgccgggg tcagcggtgt
    62101 ggttctggcg taggtgaagg ccatcatgac ccgctcaccc ggcaatacaa atgccacctg
    62161 acactggtac acctgctcgt taccccggag aaactggctc tgtatttcca aaccgcctcg
    62221 ttgagataat attccgtcat aatgtaaata gtccgtttta ataaaggaat tatttattga
    62281 caggttctgt ttttttaatc atgaatgtaa tacccatcac cgctttacag cacactttat
    62341 ctttccagtc ataatcacta ataatattaa gcaattagca atatttacaa cgaccagagc
    62401 ggatacatag ttatgtattt aacaatccca atatattcac tatgcaaata aaaccactag
    62461 taacagacac gctaccgcct ccatataaaa atggaca
//
LOCUS       DAJTUP010000010        61804 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTUP010000010 DAJTUP010000000
VERSION     DAJTUP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61804
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.010"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..334)
                     /locus_tag="OO468_000648"
     CDS             complement(<1..334)
                     /locus_tag="OO468_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR6257879.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELT"
     gene            complement(518..1936)
                     /gene="gltD"
                     /locus_tag="OO468_000649"
     CDS             complement(518..1936)
                     /gene="gltD"
                     /locus_tag="OO468_000649"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR6257880.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(1949..6409)
                     /gene="gltB"
                     /locus_tag="OO468_000650"
     CDS             complement(1949..6409)
                     /gene="gltB"
                     /locus_tag="OO468_000650"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR6257881.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            7084..8013
                     /locus_tag="OO468_000651"
     CDS             7084..8013
                     /locus_tag="OO468_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR6257882.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            8109..10445
                     /gene="arcB"
                     /locus_tag="OO468_000652"
     CDS             8109..10445
                     /gene="arcB"
                     /locus_tag="OO468_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR6257883.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            10675..11328
                     /gene="elbB"
                     /locus_tag="OO468_000653"
     CDS             10675..11328
                     /gene="elbB"
                     /locus_tag="OO468_000653"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR6257884.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            11325..12052
                     /gene="mtgA"
                     /locus_tag="OO468_000654"
                     /pseudo
     CDS             11325..12052
                     /gene="mtgA"
                     /locus_tag="OO468_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(12049..12681)
                     /gene="yrbL"
                     /locus_tag="OO468_000655"
     CDS             complement(12049..12681)
                     /gene="yrbL"
                     /locus_tag="OO468_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR6257885.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(12894..13166)
                     /gene="npr"
                     /locus_tag="OO468_000656"
     CDS             complement(12894..13166)
                     /gene="npr"
                     /locus_tag="OO468_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR6257886.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            complement(13163..14017)
                     /gene="rapZ"
                     /locus_tag="OO468_000657"
     CDS             complement(13163..14017)
                     /gene="rapZ"
                     /locus_tag="OO468_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR6257887.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            complement(14063..14554)
                     /gene="ptsN"
                     /locus_tag="OO468_000658"
     CDS             complement(14063..14554)
                     /gene="ptsN"
                     /locus_tag="OO468_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR6257888.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            complement(14672..14959)
                     /gene="hpf"
                     /locus_tag="OO468_000659"
     CDS             complement(14672..14959)
                     /gene="hpf"
                     /locus_tag="OO468_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR6257889.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            complement(14982..16415)
                     /gene="rpoN"
                     /locus_tag="OO468_000660"
     CDS             complement(14982..16415)
                     /gene="rpoN"
                     /locus_tag="OO468_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR6257890.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            complement(16463..17188)
                     /gene="lptB"
                     /locus_tag="OO468_000661"
     CDS             complement(16463..17188)
                     /gene="lptB"
                     /locus_tag="OO468_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR6257891.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            complement(17195..17752)
                     /gene="lptA"
                     /locus_tag="OO468_000662"
     CDS             complement(17195..17752)
                     /gene="lptA"
                     /locus_tag="OO468_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR6257892.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            complement(17721..18296)
                     /gene="lptC"
                     /locus_tag="OO468_000663"
     CDS             complement(17721..18296)
                     /gene="lptC"
                     /locus_tag="OO468_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR6257893.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            complement(18293..18859)
                     /gene="kdsC"
                     /locus_tag="OO468_000664"
     CDS             complement(18293..18859)
                     /gene="kdsC"
                     /locus_tag="OO468_000664"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR6257894.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            complement(18880..19866)
                     /gene="kdsD"
                     /locus_tag="OO468_000665"
     CDS             complement(18880..19866)
                     /gene="kdsD"
                     /locus_tag="OO468_000665"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR6257895.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            complement(19880..20845)
                     /locus_tag="OO468_000666"
     CDS             complement(19880..20845)
                     /locus_tag="OO468_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR6257896.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            21055..21864
                     /gene="mlaF"
                     /locus_tag="OO468_000667"
     CDS             21055..21864
                     /gene="mlaF"
                     /locus_tag="OO468_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR6257897.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21872..22654
                     /gene="mlaE"
                     /locus_tag="OO468_000668"
     CDS             21872..22654
                     /gene="mlaE"
                     /locus_tag="OO468_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR6257898.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            22659..23210
                     /gene="mlaD"
                     /locus_tag="OO468_000669"
     CDS             22659..23210
                     /gene="mlaD"
                     /locus_tag="OO468_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR6257899.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            23229..23864
                     /gene="mlaC"
                     /locus_tag="OO468_000670"
     CDS             23229..23864
                     /gene="mlaC"
                     /locus_tag="OO468_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR6257900.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            23864..24157
                     /gene="mlaB"
                     /locus_tag="OO468_000671"
     CDS             23864..24157
                     /gene="mlaB"
                     /locus_tag="OO468_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR6257901.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            24317..24571
                     /gene="ibaG"
                     /locus_tag="OO468_000672"
     CDS             24317..24571
                     /gene="ibaG"
                     /locus_tag="OO468_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR6257902.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            24626..25885
                     /gene="murA"
                     /locus_tag="OO468_000673"
     CDS             24626..25885
                     /gene="murA"
                     /locus_tag="OO468_000673"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR6257903.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(25933..26211)
                     /gene="sfsB"
                     /locus_tag="OO468_000674"
     CDS             complement(25933..26211)
                     /gene="sfsB"
                     /locus_tag="OO468_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR6257904.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(26438..27409)
                     /gene="ispB"
                     /locus_tag="OO468_000675"
     CDS             complement(26438..27409)
                     /gene="ispB"
                     /locus_tag="OO468_000675"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR6257905.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            27667..27978
                     /gene="rplU"
                     /locus_tag="OO468_000676"
     CDS             27667..27978
                     /gene="rplU"
                     /locus_tag="OO468_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR6257906.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            27999..28256
                     /gene="rpmA"
                     /locus_tag="OO468_000677"
     CDS             27999..28256
                     /gene="rpmA"
                     /locus_tag="OO468_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR6257907.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            28383..29348
                     /locus_tag="OO468_000678"
     CDS             28383..29348
                     /locus_tag="OO468_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6257908.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            29364..30536
                     /gene="cgtA"
                     /locus_tag="OO468_000679"
     CDS             29364..30536
                     /gene="cgtA"
                     /locus_tag="OO468_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR6257909.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(30722..32155)
                     /gene="dacB"
                     /locus_tag="OO468_000680"
     CDS             complement(30722..32155)
                     /gene="dacB"
                     /locus_tag="OO468_000680"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6257910.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            32403..32879
                     /gene="greA"
                     /locus_tag="OO468_000681"
     CDS             32403..32879
                     /gene="greA"
                     /locus_tag="OO468_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR6257911.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            complement(33035..33328)
                     /gene="yhbY"
                     /locus_tag="OO468_000682"
     CDS             complement(33035..33328)
                     /gene="yhbY"
                     /locus_tag="OO468_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR6257912.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            33454..34083
                     /gene="rlmE"
                     /locus_tag="OO468_000683"
     CDS             33454..34083
                     /gene="rlmE"
                     /locus_tag="OO468_000683"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR6257913.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            34183..36117
                     /gene="ftsH"
                     /locus_tag="OO468_000684"
     CDS             34183..36117
                     /gene="ftsH"
                     /locus_tag="OO468_000684"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR6257914.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            36207..37055
                     /gene="folP"
                     /locus_tag="OO468_000685"
     CDS             36207..37055
                     /gene="folP"
                     /locus_tag="OO468_000685"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR6257915.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            37048..38385
                     /gene="glmM"
                     /locus_tag="OO468_000686"
     CDS             37048..38385
                     /gene="glmM"
                     /locus_tag="OO468_000686"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR6257916.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            38613..38945
                     /gene="secG"
                     /locus_tag="OO468_000687"
     CDS             38613..38945
                     /gene="secG"
                     /locus_tag="OO468_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR6257917.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            38960..39046
                     /locus_tag="OO468_000688"
     tRNA            38960..39046
                     /locus_tag="OO468_000688"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38994..38996,aa:Leu,seq:gag)
     gene            39524..41131
                     /locus_tag="OO468_000689"
                     /pseudo
     CDS             39524..41131
                     /locus_tag="OO468_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(41139..42482)
                     /gene="argG"
                     /locus_tag="OO468_000690"
     CDS             complement(41139..42482)
                     /gene="argG"
                     /locus_tag="OO468_000690"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR6257918.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            42830..42906
                     /locus_tag="OO468_000691"
     tRNA            42830..42906
                     /locus_tag="OO468_000691"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42864..42866,aa:Met,seq:cat)
     gene            43113..43565
                     /gene="rimP"
                     /locus_tag="OO468_000692"
     CDS             43113..43565
                     /gene="rimP"
                     /locus_tag="OO468_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR6257919.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            43593..45080
                     /gene="nusA"
                     /locus_tag="OO468_000693"
     CDS             43593..45080
                     /gene="nusA"
                     /locus_tag="OO468_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR6257920.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSAELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            45105..47753
                     /gene="infB"
                     /locus_tag="OO468_000694"
     CDS             45105..47753
                     /gene="infB"
                     /locus_tag="OO468_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR6257921.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            47917..48318
                     /gene="rbfA"
                     /locus_tag="OO468_000695"
     CDS             47917..48318
                     /gene="rbfA"
                     /locus_tag="OO468_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR6257922.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            48318..49262
                     /gene="truB"
                     /locus_tag="OO468_000696"
     CDS             48318..49262
                     /gene="truB"
                     /locus_tag="OO468_000696"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR6257923.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            49411..49680
                     /gene="rpsO"
                     /locus_tag="OO468_000697"
     CDS             49411..49680
                     /gene="rpsO"
                     /locus_tag="OO468_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR6257924.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            49927..52062
                     /gene="pnp"
                     /locus_tag="OO468_000698"
     CDS             49927..52062
                     /gene="pnp"
                     /locus_tag="OO468_000698"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR6257925.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            52171..53055
                     /gene="nlpI"
                     /locus_tag="OO468_000699"
     CDS             52171..53055
                     /gene="nlpI"
                     /locus_tag="OO468_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR6257926.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            53162..53242
                     /gene="yrbN"
                     /locus_tag="OO468_000700"
     CDS             53162..53242
                     /gene="yrbN"
                     /locus_tag="OO468_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR6257927.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            53235..55124
                     /gene="deaD"
                     /locus_tag="OO468_000701"
     CDS             53235..55124
                     /gene="deaD"
                     /locus_tag="OO468_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR6257928.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            55278..56522
                     /gene="mtr"
                     /locus_tag="OO468_000702"
     CDS             55278..56522
                     /gene="mtr"
                     /locus_tag="OO468_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR6257929.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(56640..57647)
                     /locus_tag="OO468_000703"
     CDS             complement(56640..57647)
                     /locus_tag="OO468_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR6257930.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(57728..58606)
                     /locus_tag="OO468_000704"
     CDS             complement(57728..58606)
                     /locus_tag="OO468_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6257931.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            complement(58615..59610)
                     /locus_tag="OO468_000705"
     CDS             complement(58615..59610)
                     /locus_tag="OO468_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR6257932.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            59819..60343
                     /locus_tag="OO468_000706"
     CDS             59819..60343
                     /locus_tag="OO468_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR6257933.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            60337..60840
                     /locus_tag="OO468_000707"
     CDS             60337..60840
                     /locus_tag="OO468_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6257934.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(60827..61129)
                     /gene="yhbQ"
                     /locus_tag="OO468_000708"
     CDS             complement(60827..61129)
                     /gene="yhbQ"
                     /locus_tag="OO468_000708"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR6257935.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            61180..61623
                     /locus_tag="OO468_000709"
     CDS             61180..61623
                     /locus_tag="OO468_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR6257936.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            complement(61603..>61804)
                     /gene="yhbO"
                     /locus_tag="OO468_000710"
     CDS             complement(61603..>61804)
                     /gene="yhbO"
                     /locus_tag="OO468_000710"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6257937.1"
                     /translation="PQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQL
                     VTSRTPDDLPAFNREALRLLGA"
BASE COUNT        14655 a        15292 c        16788 g        15069 t
ORIGIN      
        1 gtgttaattc gccagacact gtctggcgaa tttcaacatg ttgaaaaaag aggtaaaaaa
       61 gcctcatttg gcgtaagttt tttgcagaga ccccacgttt ataccgtcga cttaaatcct
      121 tcgatagccg atcaatcaac tgtgcaccat aagctgctct ggcctcacca ccttgttcaa
      181 attcgacaat ccgtcggcca atttcccagt acgtcgcggt catcagcgcg ttaacgctac
      241 gtaccgtttc cgttcgggcg ctatcgacca gatgaataat gccgttgtgg atttgctggt
      301 agcctgttga ggttctctct gagagcgatt ccattatctg tccctttata tggcgctgcg
      361 ttgaataatg gaatcagcta tacgccgggc atctaccggt aacaatccag atgagcgtat
      421 tacattggtt ttcttcagat tatgcgaggc gatttccaga aagaaaggag aatttcagcg
      481 ccgactcttt tctgaggagg cggcgctggg acagagctta aacttccagc cagttcataa
      541 tgccgtctgc cgctttgcga ccttcggcga tggcggtaac gaccagatcg gaaccacgga
      601 cgatatcgcc gccagcaaag attttcgggt tgctggtctg gaaagcgttg tcgttgcctt
      661 ccggagcgat gatgcggcct tgtgaatcca gctcgacgct gtgttttgcc agccattcca
      721 tgttgtgtgg acggaaacca aacgccatga tcaccgcatc tgccggaacg atatgttcgg
      781 aacctgcaac gatctccgcg cggcgacggc ctttggcatc cggttcgccc atttcggtac
      841 gtaccatttt tacgccgctg actttgccgt taccgttcac ttcaatcccc agtggctgga
      901 cgttgaattt gaactctacg ccttcttccc gcgcgttttt cacttcgcgg cgggaacccg
      961 gcatgttctc ttcatcacga cgataggcac aggtaacgtg ctttgccccc tggcgtacag
     1021 acgtgcgcac gcagtccatc gcagtgtcgc caccgccaag tactaccacg cgtttgcctt
     1081 ccatgctgac gaacggttcg tcgcgggttt caccaaagcc cattaactgt ttggtgttgg
     1141 cgataaggaa cggcagcgct gcgtacacgc catcggcgtc ttcgttttcc agcccgccgc
     1201 gcattgactg ataagtccca acgccaagga acacggcatc gtaatcactc agcagatcgt
     1261 ccagctgtac gtcgcggccc acttcggtat tgagtttgaa ttcaataccc atgccggtga
     1321 agatttcacg gcgacgcgtc attacctctt tttccagctt gaaggccgga ataccgaagg
     1381 tcagcagccc gccaatttct ggatgacggt cgaagacaac ggcttttacg ccgttacgcg
     1441 tcaggacatc cgcacacgcc agacctgccg ggcctgcacc gataatcgcc acttttttac
     1501 cggtttgctt cacgccggac atatccggac gccagcccat ttcgaacgct ttatcattga
     1561 tataacgctc aatgttgccg atggtcaccg caccgaactc atcgttcagg gtgcaggaac
     1621 cttcgcacag acggtcttgc gggcagacac gtccgcatac ttccggcagt gtgttggtct
     1681 gatgcgacag ttctgccgct tcaaaaatac gcccctcgtt ggcgagcttc agccagtttg
     1741 ggatgtagtt gtgtaccggg catttccact cgcagtatgg gttgccgcac gacagacagc
     1801 ggtcagcctg cgctttggcc tggccttcgg aaaacggctc gtaaatttca acaaactcaa
     1861 ttttacggat cttcagcggt ttctttggcg gatcaacgcg ctgcaggtcg ataaattgat
     1921 aaacattctg actcattgtt gctacccctt actgcgcctg cacgcgcaac tctgctgcgc
     1981 tacgactacg gtgacccagc agtgctttta catcactgga cttcggttta accagcgcga
     2041 atttcgtggc gaaggttgac cagttcgcca gaatctcttc accgcgctga gaaccggtat
     2101 gctgaacatg ctcggtgata agaccgcgca gatgctcttc atggatcgcc agatcgtcga
     2161 cgcttaagac ttcgaccagt tccgggttaa cgcgtttgcg gaaatcaccg ctttcatcga
     2221 gaacgtaagc gaagccgccg gtcatgcccg cgccgaagtt aacgccggtt ttacccagaa
     2281 tacagacaat accgcccgtc atatattcac agccgttgtc gccaatgcct tctaccacgg
     2341 tgatagcacc ggagttccgc acgccgaaac gttcacccgc gcggcctgcg gcatacagac
     2401 gaccaccggt cgcgccatac aggcaggtgt tgccgataat gcttgcttca tggctgcgga
     2461 aggcggaacc aaccggagga cgaatggcga ttaagccgcc cgccatgcct ttaccgacat
     2521 agtcgttggc atccccggtc aggtacagtt ccacgccgcc cgcgttccac acaccgaaac
     2581 tctggcctgc ggtgccgttg aagtacgctt tgataggatc ggctgccagc ccctgatcgc
     2641 cgtgcgtctg ggcgatatag cctgaaagcg acgcgccgac agaacggtcg gtgttgcgga
     2701 tatcgaacca gaaggttttg ctctggcgtt catcgacaaa cggtttcgcc tgttgcagca
     2761 actgcgcgtt cagtaggccg ttatcaaacg gcgggttgtt ttcagtgcag tagagtgact
     2821 tacctgcatg tggctcggca gtctccagca gcttcgacag cgccagtttc tgctgtttgg
     2881 cggtgaaacc gtccagctct ttcagcaggt cggtgcgacc aatcagatcc accagacgcg
     2941 ttactcccag ctgtgccatc agctcgcggg tttcacgggc gataaactca aagtaattag
     3001 tcaccttgaa cggcaggccg tgatagtgat tcttacgcag tttgtcatcc tgagttgcta
     3061 cacccgtggc gcagttgttc aggtggcaaa tacgcagata tttacagccg agcgccacca
     3121 ttgggccagt gccgaagccg aagctttctg cgccgagaat cgccgctttg atgatatcga
     3181 cacccgtttt caggccgcca tcaacctgca aacgaatttt atgacgcaag ccgttggcaa
     3241 ccagcgcctg ttgggtttcc accagaccga gttcccacgg gcagcctgca tatttcactg
     3301 acgacagcgg gcttgcgccg gtgccgccgt catagcccgc gatggtgatc aagtccgcat
     3361 aagctttcgc cacgccagtc gcgatggtgc caacgcctgg ttcggacacc agcttaacgg
     3421 agatcatcgc tttcgggtta acctgcttga ggtcgaaaat aagctgcgct aagtcttcga
     3481 tagagtagat atcgtggtgc ggcggcgggg agatcagcgt cactccgggc accgaatagc
     3541 gcagtttggc gatgtaagga gtgactttgt cgcccggcaa ctgaccacct tcgcccggct
     3601 tcgcgccctg ggcgacttta atctgaatga cgtcggcatt gaccagatac gccggagtaa
     3661 cgccgaagcg accagaagcc acctgcttga tgcgcgacac tttgttagtg ccatagcgcg
     3721 ccgggtcttc gccgccttca ccggagttcg agttaccgcc gatgctattc atcgcttccg
     3781 ccagcgcctc gtgggcttcc gggcttaacg cgccgataga catcgcggcg gtatcaaagc
     3841 gtttaaacag ttcgcttgcc ggttcaacat cagcaatgtt gaccgcgttt tcacccggcg
     3901 taattgccag cagatcgcgc agcgtggttg ccggacgctc attaaccagc ttcgcgtatt
     3961 cctgatagtc gctgtactcg ccgctttgta ccgcctgttg cagcgtgcgc accacgtccg
     4021 ggttgtaggc gtggtattcg ccgccgtgga cgtatttcag caggccgccc tggctaatcg
     4081 gcttgcgcgc cagccaggca cgtttcgaca gattcagcag atcctgctgg aagtcttcaa
     4141 agcttgcccc accaatgcgg ctgaccgcgc cctggaagca caggcccact acatcatcgt
     4201 gtagaccgac cgcttcaaac agtttcgagc agcggtaaga ggcgatggtg gagatgccca
     4261 ttttggacat gattttgtac aagcctttgt tgatgccgtt acggtagttg agcatcacgg
     4321 tacgataatc tttggcaatc gcgtgggtgt ctaccaggcg gcccagcgtt tcataggcaa
     4381 ggtacggata aatagccgtc gcgccgaaac ccagcaacac ggcgaagtgg tgcggatcgc
     4441 gggcgctggc ggtttcgacg atgatgttgg catcgcaacg caggctttga tcgaccagac
     4501 gggtctggat cgcgccaacc gccatcggag ccggaaccgg caggcgatct ttagcgatat
     4561 tccggtcgga gagcaccagc aacacggtgc cgctacgtac cattttttcg gctttgtcgc
     4621 atagctcttt gactgtcgct tcgagcgtgg ttttagtgac gtcaaaggtg atatccagcg
     4681 tatctgcgcg gtagtgctcc tctttcatcg tcgtgagctg tttgaaatcg gagtagagca
     4741 gaatcggcga tttaaagctt aaacggtgcg cctggccctc tgcttcgcaa aagacgttca
     4801 tttcacgacc gatactggtg gcgagcgaca taacatgcgc ttcacgcagc gggtcgattg
     4861 gcgggttagt cacctgggca aactgctggc ggaagtagtc gtaaataatg cgcggctgac
     4921 tggagagcac ggcgaatggg gtatcatcgc ccatcgaacc gaccgcttcc tgaccgtttt
     4981 cgcccagtac gcgaattacg gagtccagct cttccgcgct gtagttaaac tgtttctggt
     5041 agctggcaag cgtgtcgtcg tccagttcgc ggctacccac ttcttcatcg ggcagatctt
     5101 caaacggtac cagtcggcgg acgtttttct ccatccactc tttataaggg tggcggcttt
     5161 tcagatcgtc atcggtttct gccgagtgca gaatacgccc actgcgagtg tcgatcacca
     5221 tcagttcgcc tggcccgacg cggccttttt cgaccacttc gtcaggctgg taatcccaga
     5281 taccgacttc agaggcgcag gtgatgagct tgtctttggt aatgacgtag cgtgccggac
     5341 gcagaccgtt acggtcgagg ttacaggcag cgaagcgacc atcggacatc acgatgcccg
     5401 ccggaccatc ccacggctcc atatgcatgg agttaaagtc aaagaaggca cgcagttccg
     5461 ggtccatatc cgggttgttc tgccaggcgg gtggcactaa caggcgcatg gcgcggatga
     5521 tatccatccc gcctgccagc agcagttcca gcatgttatc catggaactg gagtcagaac
     5581 cggtttcgtt gacgaatggc gcggcgtcgt gcaggtcagg gatcagcggc gtctggaatt
     5641 tataggtacg cgcacgcgcc cattggcggt taccggtgat ggtgttgatt tcaccgttat
     5701 gcgccagata gcggaacggt tgcgccagcg gccagcgcgg tacggtgtta gtggagaagc
     5761 gctggtggaa caggcaaatg gccgattcca gacgcaggtc cgcaagatcc agataaaagc
     5821 gcggcagatc cgccggcata cacagacctt tatagatgtt caccagattc gacaggctac
     5881 agacgtagaa gtctttgtcg gcttcgagac gcttttcaat gcggcggcgg gcgataaaca
     5941 gacggcgctc catatcgcgt ggacgccagc ctgccggggc gttcacaaaa atttgctcaa
     6001 tgcgtggcag agaggagagg gcgatttcac ccagcacgcc ttcgttagtg gggacatcac
     6061 gccagcccac aatcgacaag gtttcgcgtt gcagttcttc ttcaacgatg cggcgtgcgg
     6121 cagcggcgag ttcaggatct ttattcagga agagcatccc gacagcgtag ttttttgcta
     6181 aacgccagcc gcgctcctgc gcaacgatgc gaaaaaagcg atccggtttt tgtaacagca
     6241 agccgcaacc gtcgccggtt ttaccatcgg cgagaatcgc gccacggtgc tgcatgcggg
     6301 ccagtgcgtg tattgcagta cgcactacct tgtggctagg ttcgccttct atgtgggcga
     6361 tcaggccgaa accacagtta tccctctcaa gggatttatc gtacaacata tcgtgaacct
     6421 cccccaggct ctgcgggaac cccacttccg aaccgctgcg cacgggcaca gaaagagcgt
     6481 ggcgacgggg tttgcgtgtc atacgcgcgc ctcgcattcg cccccttgtt gtccttttcg
     6541 catcggttaa tacggtgaag aggggatctc aattactgca taaatatgat gagcaggctg
     6601 ctcatccaga aagcttccag cggatttcca acttatcggg aaagctgata caggtcaaat
     6661 ggcaagctta ttggtacaga aatgtgcttt agcgcaaaat tctttattaa atagctgaaa
     6721 ttaaaataga ttttatcttt aaggctaaaa ggagagtcgt tagagaaaca gtcaattcct
     6781 gactgtcata aaggcgtgtt aacccttatc ctgccttgaa ctgacgagaa aaccagcatt
     6841 ttatactgcc ttaattggta atgacgtttc atgctactgt ttttgcctaa aatccataag
     6901 gagataaaaa aatctgacac aagataagca aaagtgattt atgtgattgt gcatgaaatt
     6961 tcggtaattt tgattgatta tgcagttgat aaaagcaggc cgggacgatt gatccaggtc
     7021 atcgccgaat tgagctaaaa atggcaggct ttggcctctt ttcaaggcgc ggtctatcag
     7081 attatgcagt tacagaaatt agtcaatatg tttggtggtg atctcacccg tcgttatggg
     7141 caaaaggtgc ataagctgac gctgcacggc ggttttagct gccctaaccg tgacggtacc
     7201 atcgggcgtg gtggctgcac attctgtaat gttgcctcgt ttgccgatga agcgcagcag
     7261 catcgttcca ttgccgagca actggcgcac caggcgaatt tagttaaccg cgctaaacgc
     7321 tatctggcct actttcaggc gtataccagc acctttgcgg aagttcaggt gctgcgttct
     7381 atgtatcagc aggcggtgag ccaggccaat attgtcggtt tgtgtgttgg tacccgcccg
     7441 gactgcgtgc cggatgcggt gctggatctg ctttgcgaat ataaggacca gggttacgaa
     7501 gtgtggctgg agctggggct acaaaccgcc cacgacaaaa cactgcatcg catcaaccgc
     7561 ggccatgatt ttgcctgtta tcagcgtaca acccagctgg cacgtcagcg cgggctgaag
     7621 gtgtgttccc acttaattgt cggcctgcct ggcgaagggc aggctgaatg tttgcaaacg
     7681 ctggagcgag tggttgaaac cagcgtggac ggcataaagc tgcatccgct gcatattgtg
     7741 aaaggcagca ttatggcgaa agcctgggaa gcggggcgtt tgaacggtat tgaactggag
     7801 gattatacgc tcactgccgg agaaatgatt cgccatacgc caccggaagt gatctaccac
     7861 cgcatttccg ccagcgcccg tcgcccgacg ctgcttgctc cgctgtggtg cgaaaatcga
     7921 tggacgggaa tggtcgagct ggatcgctat ctgaatgaac atggcgtaca gggatcggcg
     7981 ctgggacgtc cgtggctacc tccaacggag taggtcgttg aggggaattc cgcatttctc
     8041 acacaattta taacgtaact gtcagaattg ggtattattg gggcaggttg tcgtgaagga
     8101 attccctaat gaagcaaatt cgtctgctgg cgcagtatta tgttgacctg atgatgaagt
     8161 taggtctggt gcgcttctca atgttgctgg cgctggccct cgtcgttctt gccattgtgg
     8221 tacaaatggc ggtaaccatg gtgctgcatg gtcaggtcga aagcattgat gttattcgtt
     8281 ctatcttctt tggtttgctg attacgccgt gggcggtcta ctttctatcg gtggtcgtcg
     8341 agcaactgga ggagtcacga caacgtctgt cacggctggt gcaaaaactg gaggagatgc
     8401 gcgagcgcga tttgagcctc aacgttcagt taaaagataa tattgcccag ctaaatcagg
     8461 aaattgccgt tcgtgaaaaa gcggaagcag aactgcagga aaccttcggc caactgaaaa
     8521 ttgaaatcaa agagcgcgaa gagacacaaa ttcagctcga gcagcaatcc tcattcttac
     8581 gttccttcct tgatgcttca cccgacttgg ttttttatcg taacgaagat aaagagtttt
     8641 ccggctgtaa ccgcgcgatg gagctgctga ccggaaaaag cgaaaaacaa ctggttcacc
     8701 tgaaacctgc tgatgtttac tcaccggaag ccgccgcaaa agtcattgaa accgatgaaa
     8761 aagtgttccg tcataatgtg tcactgacct atgaacagtg gctggattac ccggacgggc
     8821 gcaaagcctg ctttgaaatc cgtaaagtgc cgtactacga ccgcgtgggt aaacgtcacg
     8881 gtttgatggg ctttggtcgc gacattaccg agcgtaagcg gtatcaggat gcgcttgaac
     8941 gcgccagccg cgacaaaacg acgtttattt ccaccatcag tcacgaattg cgtacaccgc
     9001 tgaacggtat cgtcggcctg agccgcattc tgctggatac cgaactcacc gccgagcagg
     9061 aaaaatatct caaaactatc catgtttcgg ccgtcacgct ggggaatatc tttaacgata
     9121 ttatcgacat ggataagatg gaacggcgca aggtccagct tgataatcag ccggttgatt
     9181 tcaccagctt ccttgccgat ctggaaaatc tctccgcatt gcaggcgcaa caaaaaggat
     9241 tgcgctttaa cctggagcct acgctgccat taccgcatca ggtcattacc gacgggacgc
     9301 gtttacggca gatcctgtgg aacctcatca gtaacgccgt caaattcacc cagcaaggcc
     9361 aggttaccgt gcgcgtgcgc tacgatgaag gcgatatgct gcattttgaa gtggaagatt
     9421 ctggtatcgg cattccgcag gatgagctgg ataaaatctt cgccatgtat taccaggtga
     9481 aagacagtca tggcggtaaa cctgccaccg gcaccggtat tggtctggcc gtttctcgtc
     9541 gtctggcgaa aaatatgggc ggcgatatta cggttaccag tgaacagggc aaaggttcaa
     9601 cctttacgtt gacgatccac gcaccgtcgg tggcagaaga ggtcgatgat gcgtttgatg
     9661 aagacgatat gcctttaccg gcgctgaatg tgctgctggt ggaagacatt gaactgaacg
     9721 tgattgtcgc gcgttctgtg ctggaaaaat taggtaacag cgttgatgtc gccatgaccg
     9781 gcaaggcggc gctggagatg tttaaaccgg gcgaatacga cctggtgttg ctggatattc
     9841 agttgccaga tatgaccggg ctggatatct ctcgtgaact gacgaagcgt tatccgcgcg
     9901 aggatttacc gccgctggtg gccttaaccg ctaacgtgct gaaagacaaa caagagtacc
     9961 tcaatgctgg aatggatgat gtgctgagta agccgctttc ggttccggcg ctaaccgcga
    10021 tgatcaagaa attctgggat acccaggatg atgaggagag tacggtgacg acagaagaga
    10081 acagtaaatc agaagcattg ctcgatattc ccatgctgga acagtatctc gaacttgtag
    10141 gaccgaagct gatcaccgac gggttagcgg tgtttgagaa gatgatgccg ggctatgtca
    10201 gcgtgctgga gtcgaatctg acggcgcagg ataaaaaagg cattgttgag gaaggacata
    10261 aaattaaagg tgcggcgggg tcagtggggt tacgccatct gcaacagctg ggtcagcaaa
    10321 ttcagtctcc tgaccttccc gcctgggaag ataacgtcgg tgaatggatt gaagagatga
    10381 aagaagagtg gcgtcacgac gtagaagtgc tgaaagcgtg ggtggcaaaa gccactaaaa
    10441 aatgaccccg gctagaccgg ggtgcgcgaa tactgcgcca acaccaggga aatcttggct
    10501 gcgccgtaaa ttattatgat gagttacaag ggcacagcac tgtttttcag gccgcacgca
    10561 gttaagatag caaaacttaa atgatttgtt acatgaatca gttaaatgtg tgatgcgtac
    10621 catttttatt aaaaaaatta atgtgtttca gtcaattgcg aaaggatgat cacaatgaag
    10681 aaaattggcg taattctgag cggatgcggc gtctatgacg gttctgaaat tcatgaagcg
    10741 gtgttgacgt tgttagctat ttcacgcagc ggtgcgcagg cggtctgctt tgcaccggat
    10801 aagcagcagg ttgatgttat caaccattta actggcgaag cgatgacgga aacgcgcaat
    10861 gtgctgattg aagcggcacg aataacgcgc ggtgaaatcc gtcctctggc ccaggccgat
    10921 gccgctgaac tggatgcgtt gattgtgccg ggggggtttg gcgcggcgaa gaatttaagc
    10981 aattttgcca gtcttggtag cgaatgcacc gttgaccgtg aattaaaggc gctggcacaa
    11041 gcgatgcatc aggccggaaa accgcttggt tttatgtgta ttgccccggc gatgctgccg
    11101 aaaattttcg atttcccgct gcgtttgacc atcggtactg atatcgatac cgcagaagtg
    11161 ctggaagaga tgggcgcgga gcatgtgccg tgtcctgtcg atgatatcgt ggttgatgaa
    11221 gacaataaga ttgtcaccac cccagcatat atgctggcgc agaacattgc agaagcggcg
    11281 agcggcattg ataagctggt ttcccgcgtg ctggttctgg ctgaatgagt aaaagccgct
    11341 taacggtgtt tagtttcgtt cgccgttttc ttttgcggtt aatggttgtc ctcgccgttt
    11401 tctggggcgg gggcatcgcg ttgtttagcg ttgcgcctgt tcccttctca gcggtaatgg
    11461 tcgagcgaca ggtcagcgcc tggctgcatg gcaattttcg ttacgtggca cattctgact
    11521 gggtcagtat ggatcaaatc tcgccgtgga tgggactggc ggtgattgcc gcagaagatc
    11581 agaaatttcc tgagcactgg ggctttgatg tcgcttccat tgagaaagcc ctggcgcaca
    11641 acgagcgcaa tgaaaaccgt attcgcggtg cttcaacgat ttctcagcag acggccaaaa
    11701 tctcttttta tgggatgggc gtagctgggt acgaaaaggg ctggaagctg gattaacgct
    11761 ggggatagaa acagtctgga gcaaaaagcg tatcctgacg gtttacctga atatcgccga
    11821 atttggcgac ggtgtgtttg gcgtcgaagc tgcggcacaa cgttatttcc acaaacccgc
    11881 gagcaaactt acccggtcgg aagctgcatt actggcagct gtattaccta atccacttcg
    11941 tttcaaagtc tcctcgccat cgggctacgt gcgtagccgt caggcgtgga ttttacggca
    12001 gatgtatcag ttaggcggtg agccgtttat gcaacaacac cagctggatt aagcttcgcg
    12061 cgaagtgagg gccagcgttt tgctctcacg aggttgcaga tctttctgca gggcaatagc
    12121 cagcgccggt tgggcgataa atcgtttcca taaccgctct tgcttacgca gacaacacca
    12181 cttcgaccag gtcgccagtg gaatcaatgt gctttcacca atgttgtcgc agacgaccgg
    12241 gataacctct gactcactga tgcggtgaca gaggatattc tgcggcttca gcgacatcgt
    12301 cacgatacgg ttatcctgca aataacgctt cagctgtttc agtagctggc gcagttgtgc
    12361 aatgtcttct tcgtaacggc attgttcagc aaattcggtc agcgtaatgg aaggcttgcc
    12421 gtcaaaatcg gcaatcacat cgtagacata accggttccg caatccgtct caacggtacc
    12481 gtgatagcgc ggtattccac tccagtcttt taaccggcga ccaagatgcg cgtagtactt
    12541 taactcacgg cggatctctt tatcgccacc atcgccacga tggtagacaa tcttgataca
    12601 gcgttgggca tcttccggat gcgcataaca tttccgatgt cttccggtgc ccaggggact
    12661 ttgttcagat aagcgaatca tgccatctcc tggaaatatt ttctgacgat ctccagtatg
    12721 gtcgattaac ttaaacaaaa cctaaacaat gcctcttaag gttttcttaa ggttcttctg
    12781 aaagtgaaaa gcgcacaaaa tgtacaaatg acgttgattg agaacgattt cagtacgtga
    12841 ttttcttatt gtcgggggag cttgaaggga gttgtatgtc aaagtgatga agattaatct
    12901 tcatcaaaac cagaattaaa gagggcgata acggcggcca gtgcttcctc ttcctgtgga
    12961 ccggtcgctt caacttcaat ctgccgtcct ttggcagaat ccaacatcag cagcgcaata
    13021 acgctgttgg cttcagcctc ggtgccttcg tcattacgta acagcacttc agcgtcaaaa
    13081 ccctgcatta attcaaacag cttcattgca ggccgggcat gcatgcccag cttgtttgtg
    13141 atttcaacag tttgcttgac ggtcatggtt tacgtttttc cagcgtacga tggcgtgact
    13201 ggacgttttt accgcgcgag cggaagtagt ctgccagttg ctctgcaata tacaccgaac
    13261 ggtgcttccc gccggtacaa ccaatggcga ccgtcaggta gctacggttg ttggtttcca
    13321 gcataggtaa ccatagctca agatagcttc gcgtctggta gataaaattg tgtacttctg
    13381 tgtggcggtc gaggaacgcg gcgacaggtt tatcaagacc tgtcattgga cgcagtttcg
    13441 gatcccagtg cgggttcggc aagaagcgca cgtcaaagac gtaatctgca tcgataggga
    13501 taccgtgttt gaagccgaaa gactcaaaga ccatggtcag ttcgcgttca cgtttaccca
    13561 gcagacgggt acgcagcatt tcagccagct cgtgaacgga catttctgag gtatcgacaa
    13621 tcagatccgc tcgcgaacgc agaggctcca gcaaatcgct ttctttgtcg atagcacttt
    13681 ccagcgacag gtttttgctg gaaagcggat gcagtcggcg cgtgtcactg taacgacgaa
    13741 ttaaggtatt acggtcggca tccaggaaca gtagttgcgg tgagaaagcg tcaggcaggt
    13801 tactcatcgc ctgttcgaat atttctggtg actccggcat attacgaaca tcaatgctga
    13861 cggcggcaga aatctcgcga tcggccagag ttcgagccag atcgggtaac aacactacgg
    13921 gaaggttatc cacgcagtaa aaacccatat cttccagcgc acgcagggcg acagatttac
    13981 ctgaacctga acgtccgctg acgatcatca gtaccatgta ctgtttctcc tcacaacgtc
    14041 taaaagagac attaccgaat aactacgctt catccggagt accttcggta tccgtaatga
    14101 tttgatacag ctcttcatcg ctctgggctg cgcgtaaacg gcggcagatg gttttgtccg
    14161 ccagacgttt cgccaccagc gacagagtat gcaggtgcgt tttagtttgg tctgccggca
    14221 ccagcagggc aaaaagtaag tccaccggtt ggttgtcgat ggcatcgaaa gctataggcg
    14281 tttcgagctg aacgaaaacg ccaacggcac gcagagtatc ttcttcaagt ttgccatgcg
    14341 gaatggcaat accattgccg ataccggtac tgcccatttt ttcccgcgtc aggatagctt
    14401 caaaaaccac ctgaggcggc aggctaagtt gtttcgccgc cagctcgctg atgatttcca
    14461 gggcgcgttt tttgctctga cagtggacgc ggcttcgcgt acattccctg ttaagaacac
    14521 tgctaagctg tagagttgta tcattatttg tcataatttc acctaagaac ctgcccactc
    14581 aaacagtgga acaggctcgg agcattaacc gtacaaatgg cccgttgtgc cgcacaacag
    14641 gccgccatgc acatgctaat tgcccggaca attagtgttg tttcagttta tctttatgtt
    14701 tggtcaactg gcgtgccagc ttatcaatta agccatcaat ggcagcgtac atgtcctgac
    14761 cttccgcgct ggcatgaatt tcaccaccgt ttacatgcag tgttgcatct gaggtgtggg
    14821 tgactttctc cactttcaga acaacataga cctggttgat tcggtcaaaa tattgctcaa
    14881 gtttggcaaa tttagctgta acaaattcgc gcagtgcctc ggtgatctcg acgttatttc
    14941 cggtaatgtt gagctgcata gtgtcttcct tatcggttgg gtcaaacgag ctgtttacgc
    15001 tggtttgacg gcggaatgga taaagactct cggtacttcg caacagtgcg gcgtgccacc
    15061 atgatacctt gttccgacag caaagaggtt aacttgctgt cgctcaacgg tttcgctggg
    15121 ttttccgccg cgattaattt cttcaccagt gcacgaatcg ccgtggagga agcttcgccg
    15181 ccgccctcgg tattgacgtg actggaaaag aaatacttca gttcaaaaat gcctcgtgga
    15241 ctatgcaggt atttttgcgt ggtcacgcga gatatcgtcg attcatgcat ttcgacagcc
    15301 tgggcgatat cggccagtac catcggtttc atatattctt caccttgctc aaagaaggct
    15361 tgctgctgtt caacgataca gcgactcacg cgcagtagcg tatcgttacg gctttccaga
    15421 ctcttgatca accatttggc atcctgcaga ttgctgcgga taaactggct gtcaccatcg
    15481 ttgcgcgcgt tattgcacat cgaggcgtag tgctggttga tttgcagacg cggaatgctg
    15541 tcactgttga gttctaccgt ccagtgaccg ttatgcttac gcaccagcac atctggaatg
    15601 acatactcag gttcgccagt ctggatcgac tgcccggggc gcggatcgag cgactggatc
    15661 agattgacgg cttctttcag cacatcttct ttcagacgcg tgacgcgcat taaagtgcgg
    15721 aagtcgtgat tggctaacag atcgagatga tcgctaatga tcaatctggc ctcttccagc
    15781 cacggcgtgg tcttatcgaa ttgggagagt tggattagca ggcagtcacg cagatctttt
    15841 gccgctacac cgaccgggtc aaaccgttgg atccgcttaa ggacggcttc aacctcgtcg
    15901 atgtcgatct cttcatcgcc catactttcg agaatatctt ccagcgggac agtcagataa
    15961 ccggtttcat caacggcatc gacgatagag gtagcaatag cgcggtcagt gtcggaaaac
    16021 ggtgtcagct cgacctgcca catcaggtaa tcctgcaagg tctgcgtcgt ttcgccctgg
    16081 tagaccggca gctcgtcgtc aatgtagtca ccgctggtgc cggatggtgt accagcggtg
    16141 taaatggtgt cccaactggc atcgagcggc agctcttccg gcatctcttt ttgttcgagc
    16201 gcgtcggcgg tgtccagcgt ttcactgtct tgcgtttcgc gggtgtcgat ttcttcatga
    16261 gtgtcgattt gctcaagcag cggattactc tccagcgcct gctgtagctc ctgctgaagt
    16321 tccagcgtcg acaactgcaa cagacgaatt gcctgttgga gctgtggcgt catcgccagt
    16381 tgttggctaa gcctgagttg caaaccttgc ttcatgttca gaatcgtact ctcctgctaa
    16441 aacgtcgcaa acttctaccc tatcagagtc tgaagtcttc cccaaggtat acacgcttaa
    16501 cgtgttcgtc ttgtaagatt tctgtaggcg tgccgtgggc gatcaaatgc ccctgactga
    16561 cgatataagc gcgttcacaa accgccagtg tttcacgcac gttgtggtca gtgatcagca
    16621 cgcccaggcc gctgtcgcgc aggtgttcaa tgatgcgttt aatgtcgata accgagatcg
    16681 ggtcaacccc ggcaaacggt tcgtcgagca gaataaattt cggattcgca gccagtgcgc
    16741 gggcaatttc tacacggcga cgttcacccc cggagagtga ctgccccatg ctgtcacgca
    16801 ggtgctcaat gtgaaactct tccatcagct cgttcgcgcg gtcttcacgt tgttcagcag
    16861 acaagtcgtc acgaatttgc agtaccgcca tcaggttatc gtaaacgctg aggcgacgga
    16921 aaatggaggc ttcctgtggc agatagccga taccgcggcg cgcgcgtgca tgcagaggca
    16981 gcagactgat atcgtcatca tcaataatga tgttgcccgc atcgcgcggc acaatgccta
    17041 caaccatgta gaaagtggtg gtcttaccgg caccgtttgg ccccagcaga ccgacaattt
    17101 ccccggagtt gacggtcagg ctgacgtctt ctaccacgcg acggccttta taggcttttg
    17161 caaggttctt tgcagttaat gttgccataa cgaattaatt acccttcttc tgtgccgggg
    17221 tctggccttt gttgtttttg tcctgcagct gcgacggcac cagaacggtg gttacgcgct
    17281 tgcctttgtc gctgaaagcc tgcattttct gctctttcac caggtaagtg atcttatcgc
    17341 ccttaatgtt gctatcgacc tgctgcagat aagcattacc cgtcagaacg acaaaatctt
    17401 ttgccagttc gtagtgcatc tgggaagcgt gaccttcaac gggtttaccg ttgtcctgca
    17461 tctggtagaa cgttgccggt ttaccgtatc cgtcaatcac ttctttacct tgttcgccgc
    17521 ccggacgggt aacgaccact ttgtcggcat taattttgat ggtgccctgg gtgacgatga
    17581 cattaccggt aaaggtaacc acgttgcctt gcatatcaag agattgctgg tccgattcaa
    17641 tgtggatcgg ctgatcagtg tctccggtta cggcaaatgc cggaatgctg gcggccagaa
    17701 gtgagctggc aagcacaaga ttaaggctga gtttgtttgt tttgaatttc ataggatgtt
    17761 ctaacctttt caatcagctc ggcgttcttg ctgcgtaagt tgccgcgcat tttcagaccg
    17821 ctggagttaa atgttgttcc gtataacgtg acgaggtctt cagaggtaac atcctgcgtc
    17881 accagattga tctgcgcgtt atccgtcgtg attctgcgaa gttgagagtc tggcacaagt
    17941 gcgttgactt caacgtgtcc atataaatag agcatccggt cattggtcag cttggcttta
    18001 tctgctttta cggaccatgt cgggatttta tccttatcaa acgtggtaag taccggctgc
    18061 gtaaaccacg aaacggcctg atcggaataa tattcaacgt gttgagcaat caatcgatag
    18121 cttagtgccc cttctgggtt atagacgagc gtgtccgtat gctcgctttt ataggtggga
    18181 tcattgttgt tgacgaccac ctgggcggta tcgtcttttt cggccatatt aatgccgatc
    18241 atcaccagaa ccgccagtga tagcacaatg ataacccaac gtctggcttt actcatatcg
    18301 attgcccttt ggcttcatcc agtttgccct gcgccaggag taataagtcg caaacttcgc
    18361 gcactgcgcc acgaccgcca gcaatgcgcg tcacgtaatc ggcgcgcggg atcaacagtg
    18421 gatgcgcatc ggccacggcg acgcttaaac ccactttttc cattaccggc cagtcgatga
    18481 gatcatcgcc aacataagcc acattttccg gggcaatcgc caatttttcc agcaaatcgc
    18541 taaaggcgat cagtttgttt gactgcccct gatacaagtg agtgatcccc aatgtggcac
    18601 aacgatcttc taccagttta gcctttcgcc cggtaatgat agcgacttca atatcagagg
    18661 tgagcgcaca acgaatgcca taaccgtcac gaacattgaa cgctttcagc tcttcgccgt
    18721 tattgcccat ataaatcagg ccatctgaca gtacgccatc aacgtcgagg atcagcagac
    18781 gaatgttctc tgcttttgcc ataacgtcgg cgctgacagg gccgtaacag gtcgcaagcg
    18841 acgcacctgc tttgctcatt gttgtttatc cttgaatctt tacactacgc ctgcacgcag
    18901 taaatcatgc atatgtaaca caccgagtaa atggtcgcca tcggcaacca tcacggaggt
    18961 gatatggcgg gactgcatta agttcagtgc ctcaacggcc agaatgccag ggcgcacacg
    19021 tattcccccc ggcgtcatca catcggcaat acttaactga cgaacatcca cgcccatatc
    19081 gaagacacgg cgtaaatcac cgtcggtaaa gatgccttca atcatcatat tgtcatcgca
    19141 aatgacagtc ataccaagat ttttgcgggt aacttccagc aatgcgtcac gcagactggc
    19201 cgttttctta acatgcggga tctcatcgcc cgtatgcata atatcgttta cgcgcagcag
    19261 aagtttacga cccagtgcgc cgcctgggtg tgagagcgca aaatcttcag cagtaaagcc
    19321 gcgtgctttt aacagcgcga cagcgagggc atcgcccata accagcgtgg cggtggtgct
    19381 gctggtcggt gccagcccta acggacaggc ttctttcgct actttaacac acagatgcac
    19441 atctgcggcg cgcgccatgc tgctctccgg gcgaccggtg atgcagatta acggtacgtg
    19501 aagacgctta agcactggaa ttaaggccgt gatttcgctg gattcacctg agttagagat
    19561 agcaatcacc acatcctgtg gggtgaccat gcctaaatca ccatgcgcgg cttcactagg
    19621 atggacgaaa aatgaaggtg taccggtgct ggcaaacgtt gcggccattt tgcgcccgat
    19681 gtgccccgat ttccccatcc ccatgacgac aactttccct ttacaccaga acatcttttc
    19741 acaggcaagc gtgaaattct gattgatgta ttgatcaagc tccgccaggc attcacgttc
    19801 aatcgccagg acttctttac ctgcttgctg aaagtcaaaa cccggttgta actctacgtg
    19861 cgacataatg cgtttccagt tattcaacga gtattggcga taaccagtac aacatcgcca
    19921 gccatacgat aaatccgcca gttaataata cccctacacc acggcccggt tgcggggagc
    19981 gccgccagca cagcaacgca aaaataatgc tcaccagcaa catcacgctg tagtcacgac
    20041 tgtacgccag tggatcaatc tctcctggcg ttatcagcgc gggtaaaccc aacacgatga
    20101 caatattaaa aatgtttgcg ccaatgatat ttccgacagc aatgtcgttt tcacctttgc
    20161 gaacccccgc tattgcggtt gccagttccg gcagactggt tccgatagca attgccgtca
    20221 gagccatcgt caactcgctg atggcaaagt aattcgccag caccgtggcg ttatcaacca
    20281 ccatccgcgt ggccactggc atgatgataa gcgcaatgcc gagccataaa aacgcgacgg
    20341 gcaatccgcc gtcacgcggc agctctgcaa gctgctctct ggtcaggctg tcagtcccct
    20401 gacgtgcaag tttaacaatg aacagcagcc atagcacagc cagaaagagg agaaagatac
    20461 catcgcttcg actaagttgt ccgtcataga gtacggaacc ggccaccacg ctgaccaaca
    20521 acattaaggg taattcacgg cgcagaacat cggaatggac ggtaaaagga cgaaccagcg
    20581 cagccagacc gaggatcagc aatatattga taatgtttga gccgagggct gtaccaacgg
    20641 ctaaatcgcg ttgttcgtgc agagacgcgg caagcgagac gatgatttct ggtaacgatg
    20701 taccaatact gaccaccgtc atgccgatga tcagcggcgg gatgccaaag gttcggcaaa
    20761 gaatagacgc ggcaaaaacc aggcggtcgg cactgtaaac gaccaaaagt aaaccaacaa
    20821 ttaacagtgc cgtagctaaa agcatctaac gtcctttctt caggtatact cgccggtccg
    20881 ctgaagattt tcagaaagcc gtaacggatt cttaattttg actttatgcg gctaaaaagt
    20941 aaaacaaatg ccagctttcg ctaaccacgg cgggtaatat tctgtaaata tgttgggttc
    21001 aaggttaaat tgagcgccat gcttagaaaa tcaacgcaag acgaagggtg aattatggag
    21061 cagtctgtgg cgaatttagt cgatatgcgc gatgtcagtt ttacgcgtgg caatcgctgc
    21121 atcttcgata atatttccct gaccgtgccg cgagggaaga tcacggcgat catggggcca
    21181 tcgggcatcg gtaaaacgac gctactccgt ctgattggcg ggcaaatcgc accagatcat
    21241 ggtgagatcc ttttcgatgg tgagaatatt ccggcgatgt ctcgttcgcg cctgtataca
    21301 gtgcgtaaac ggatgagcat gttatttcag tccggggcgt tgttcactga tatgaacgta
    21361 tttgacaacg tcgcctatcc actgcgcgaa catacccaac ttcccgcgcc attgttgcat
    21421 agtacggtga tgatgaagct ggaggccgtg gggctgcgtg gagcggctaa actaatgcct
    21481 tctgaacttt ccggtgggat ggcgcggcgt gcagcgctgg cgcgtgcgat tgcgctggag
    21541 ccggatctca tcatgtttga tgaacctttt gttgggcaag atcccatcac catgggcgta
    21601 ctggtgaagc tgatttctga gctgaacagc gcgctgggcg tgacttgtgt ggtggtttct
    21661 cacgatgtgc cggaagtgtt aagtattgcg gatcacgcct ggatcctggc ggacaaaaaa
    21721 attgtcgctc atggcagtgc ccaggcgttg cagacgaatc ctgatccgcg cgtacgtcag
    21781 tttctggacg ggatagctga cgggcctgtt ccgttccgct atcctgccgg cgattatcac
    21841 gctgatcttt taccagggag ttaagccact catgctgtta aatgcgctgg cgtcgctcgg
    21901 acataaaggg attaaaaccc tgagaacgtt cgggcgggcc gggttaatgt tattcaatgc
    21961 gctggtcggc aaaccggaat ttcgcaaaca tgcgccgctg ctggtgcgcc agctctataa
    22021 tgtcggcgtc ctgtcgatgc tgattattgt agtttctggc gtgttcatcg gaatggtgtt
    22081 ggggctgcaa ggttatctgg ttctgaccac ttatagcgcg gaaaccagtc tgggtatgct
    22141 ggtggcgtta tcgctactgc gtgaactggg gccggtggtt gccgcgctgt tgtttgccgg
    22201 gcgtgctggt tcggcgttaa ccgcagaaat cggcctgatg cgcgctacag agcaactctc
    22261 cagtatggag atgatggcgg tggatccgct gcgtcgggtt atttctcccc gtttctgggc
    22321 tggggttatt tcattaccac tgttgacggt tatcttcgtc gccgtgggga tctggggcgg
    22381 atcgctggtc ggcgtcagtt ggaaaggcat tgatagcggg ttcttctggt cggcaatgca
    22441 aaatgccgtc gactggcgta tggatctggt caactgtctg attaagagcg tggtgttcgc
    22501 catcacggtg acgtggattt cgttgtttaa cggctacgac gccatcccga cgtctgccgg
    22561 gattagccgg gcaaccactc gcaccgttgt ccactcgtct ctggctgttc tggggctgga
    22621 ttttgtgctg accgcattga tgtttgggaa ttgagttcat gcaaacgaaa aaaaatgaaa
    22681 tttgggtggg tatcttttta ttagcagcac tgctggcggc gctgtttgtt tgcctgaagg
    22741 cggcgaacgt gacgtccata cgtactgaat cgacctacac gctttatgcg acgttcgata
    22801 acattggcgg cctgaaagcg cgctctccgg tcagcattgg tggtgttgtt gtgggccggg
    22861 tggcggatat tacgctggac ccgaaaacct atctgccgcg cgtaacgctg gaaattgaac
    22921 aacgttataa ccacattcct gataccagtt cgctgagcat tcgtacttcc ggcctgctgg
    22981 gagaacaata tctggcatta aacgtcggtt ttgaagaccc ggaactgggg actgctatcc
    23041 tgaaggatgg cgatacaatt caggacacca agtctgcgat ggtgctggaa gatctcattg
    23101 gtcagttcct ttacggtagt aaaggcgatg acaataagaa tagtggcgat gcgccagctg
    23161 ctgcgccagg taataatgaa accactgaac ctgtgggtac aacgaaataa tttcaggaga
    23221 accgacgcat gtttaaacgt ttaatgatgg tcgctttgct ggtgattgca cctctgagtg
    23281 cggcaaccgc ggcagaccag accaatcctt ataagctgat ggacgaggcg gcgcagaaaa
    23341 cgttcgatcg cctgaagaat gagcaaccgc aaattcgggc caacccggat tatctgcgta
    23401 ccattgttga tcaggaactg ctgccatacg tacaggtgaa atacgccggt gcgctggtgc
    23461 tgggccagta ttacaagagt gcgacccctg ctcaacgtga agcctacttt gccgctttcc
    23521 gtgagtacct gaagcaggct tacggtcagg cgctggcgat gtatcacggt caaacctatc
    23581 agattgcgcc agaacagccg ctgggcgata aaaccattgt gcctattcgc gttaccatta
    23641 ttgacccgaa tggccgtccg ccggtgcgtc tggacttcca gtggcgtaaa aactcccaga
    23701 cgggaaactg gcaagcttac gacatgattg ctgaaggcgt cagtatgatc accaccaaac
    23761 aaaacgagtg gggaacgctg ctgcgtacca aaggtatcga cggcctgact gcgcaactga
    23821 aatcgatttc tcaacagaaa atcactctgg aagagaaaaa ataatgagcg agtcactgag
    23881 ctggatgcag acgggtgaca cgctggcgtt atccggagag ctggatcagg acgttctgct
    23941 accgctttgg gaaatgcgtg aggaggcggt gaaggggatt acctgcatcg atcttagccg
    24001 tgtgtcccgc gtggatacgg ggggactggc actgctgctc catcttatcg atctggcgaa
    24061 aaagcagggc aacaacgtga cgcttcaggg ggtaaacgac aaagtgtata ccctcgcgaa
    24121 attgtataat ttgcctgctg atgttctgcc tcgttaaatt ttttcaggcc ggatatggcg
    24181 ttaaccgcct gtccggcaaa cctgcctgca agtcgagcct tgtctgattt atcagcgagg
    24241 ctttttgctt atttatgcca gcgcaacttt gctctaagat gtttcgctgg tttattaact
    24301 gatgattgaa gatcccatgg aaaataatga aattcagagc gtgttgatga acgctctctc
    24361 cctccaggaa gtccacgttt ccggcgatgg cagccacttt caggttattg ccgtgggtga
    24421 gttgtttgac ggtatgagtc gggttaaaaa acagcagacg gtctatggcc cgctgatgga
    24481 atatattgcg gataaccgca ttcatgctgt gtcgatcaaa gcgtataccc ctgcggagtg
    24541 ggcgcgcgat cgcaaactga acggcttttg agctatgggc gattcgccgg tagcggatat
    24601 gaattgttaa ctgagaacaa actaaatgga taaatttcgt gttcaggggc caacgaagct
    24661 ccagggcgaa gtcacaattt ccggcgctaa aaatgctgct ctgcctattc tttttgccgc
    24721 actactggcg gaagaaccgg tagagatcca gaacgtcccg aaactgaaag acgtcgatac
    24781 atcaatgaaa ctgctaagcc agctgggtgc gaaagtagaa cgtaatggtt ctgtgcatat
    24841 tgatgcccgc gacgttaatg tattctgcgc accttacgat ctggttaaaa ccatgcgtgc
    24901 ttctatctgg gcgctggggc cgctggtagc gcgctttggt caggggcaag tttcactacc
    24961 tggcggttgt acgatcggtg cgcgtccggt tgatctacac atttctggcc tcgaacaatt
    25021 aggcgcgacc atcaaactgg aagaaggtta cgttaaagct tccgtcgatg gtcgtctgaa
    25081 aggcgcacat atcgtgatgg ataaagtcag cgttggcgca acggtgacca tcatgtgtgc
    25141 tgcaaccctg gcggaaggca ccacgattat tgaaaacgca gcgcgtgaac cggaaatcgt
    25201 cgataccgcg aacttcctga ttacgctggg tgcgaaaatt agcggtcagg gcaccgatcg
    25261 tatcgtcatc gaaggtgtgg aacgtttagg cggcggtgtc tatcgcgtgc tgccggatcg
    25321 tatcgaaacc ggtactttcc tggtggcggc ggcgatctct cgtggcaaaa ttatctgccg
    25381 taacgcgcag ccagatactc tggacgccgt gctggcgaaa ctgcgtgatg ctggagcgga
    25441 catcgaagtc ggcgaagact ggattagcct ggatatgcat ggcaaacgtc cgaaggctgt
    25501 taacgtacgt accgcgccgc atccggcatt cccgaccgat atgcaggccc agttcacgct
    25561 gttgaacctg gtggctgaag ggaccgggtt catcaccgaa acggtctttg aaaaccgctt
    25621 tatgcatgtg ccagagctga gccgtatggg cgcgcacgcc gaaatcgaaa gcaataccgt
    25681 tatttgtcat ggtgttgaaa aactttctgg tgcacaggtt atggcaaccg atctgcgtgc
    25741 atcagcaagc ctggtgctgg ctggctgtat tgcggaaggg acgacggtgg ttgatcgtat
    25801 ttatcacatc gatcgtggct acgaacgcat tgaagacaaa ctgcgcgctt taggtgcaaa
    25861 tattgagcgt gtgaaaggcg agtaatcgtc tgagagctgg cagccccgtt cgcggggcta
    25921 ccgccagacc actcattttt tcggtttagt gtagctacgc atcaactgcg ttctgtcgat
    25981 aaactcatgg gtctgcggat catggtagcg tgatggccag ataacccagg ggtcagttcc
    26041 cagggctttc gcaataatca tctctccttt cggccatggg cgcgataatg cgtttgccag
    26101 cgttgaggaa ctcaaaccat ttctgcgaga ttccgccgcc attgacgttc ctttcttgcg
    26161 caaacctgca atgatgtcag cgggatgcca gtcaatgaaa ttactttcca tctttcctca
    26221 ctccttaagt tagtcgcttt cattcagaac acttcttctg tcgggcatca ctatactcct
    26281 gatggtctat tgcgctaaaa agttcctgta aagagtgtga atattcagaa tatataaaga
    26341 ttcatcgccc ttttttcagg tgttatagcg ctgaattagt gtttttatgg aatttactgg
    26401 aatcatcccg cgcactgcgc gggatgaagg gaggggatta acgatcgcgt tgaacagcga
    26461 tgtgcgcgag gccgatgagt gcttctcgcc aaggggtgtc cgggagcacc tgtaacgctg
    26521 cgatggcttt gtctgcttct tcctcggcgc gctgacgcgt ccattcaaga gatccacaag
    26581 cgttcattgc ttccagaacc ggttccagaa gatggcgacc gttaccttgt tcgatggcgg
    26641 tacggatcat ctgtgcctgt tctggtgtgc catgatgcat cgcatgcagc agcggcagcg
    26701 tcggtttacc ttcgttcaga tcgtcgccga cgtttttacc taactgttcg ccatcggcat
    26761 tgtaatcgag taaatcgtcg atcaactgga aagcagtgcc gagatagcgc ccataatcct
    26821 gcaggccttt ctcctcctcc ggcgtacagc cagccagaat cccggaacac tgcgcggcag
    26881 cctcaaacag acgcgcggtt ttgctataga taacgcgcat gtagttttct tcagtgatgt
    26941 ccggatcgtt aacgttcatc agttgcagaa cttcaccttc tgcgatgacg tttacagctt
    27001 ctgacatgac ttccagcact ttgagtgaac cgaggctggt catcatctgg aacgcgcggg
    27061 tataaataaa atcgcctacc agcacgctgg cggcattgcc aaatgcggcg ttggcggtag
    27121 ctttacccct gcgcatatct gattcatcca caacgtcgtc gtgtagcaga gtcgccgtgt
    27181 ggataaactc gatcagggca gcaatggtga catgcgcatt tccctcatag ccaacagctc
    27241 gtgcagccag tacagcaatc atcggacgaa tacgtttacc gccgccgctg acgatgtaat
    27301 agcctaactg attgatcagt tggacgtcgg aattaagctg ctcaaggatt gccgcattaa
    27361 cacccgccat atcttgcgcg gttaactcat tgattttttc taaattcatc gcaaaagccg
    27421 ggcttttcgc cccgattacc ttactgtgaa caaaggcaac ttagggttta tggttaggaa
    27481 tatagggtaa ttgtactgaa aaaatggcac agataaacgt taccgtacaa gttgtgtttt
    27541 tttcttcgtg tattgactgt agcacttgtc aaaggcgtgc gttttgcgta atattcgcgc
    27601 cctattgtga atatttatag cgcactctga atcattgaaa aggtgtgcgc ggaagcggag
    27661 ttttatatgt acgcggtttt ccaaagtggt ggtaaacaac accgagtaag cgaaggtcag
    27721 accgttcgcc tggaaaagct ggacatcgca actggcgaaa ctgttgagtt cgctgaagtg
    27781 ctgatgatcg caaacggtga agaagtcaaa atcggcgttc ctttcgttga tggcggcgta
    27841 atcaaagctg aagttgttgc tcacggtcgt ggcgagaaag ttaaaatcgt taagtttcgt
    27901 cgtcgtaaac actatcgtaa gcagcagggc catcgtcagt ggttcactga tgtgaaaatt
    27961 actggcatca gcgcctaaga cctgaggaga gatttcaaat ggcacataaa aaggctggcg
    28021 gctccacacg taacggtcgc gattcagaag ctaaacgctt gggcgttaag cgtttcggtg
    28081 gcgaatccgt tctggcgggt agcatcatcg ttcgtcaacg tggtaccaaa ttccacgctg
    28141 gcgctaacgt aggttgcggt cgtgaccaca ctctgtttgc taaagcagac ggtaaagtga
    28201 aattcgaagt taaaggcccg aaaaaccgta aatttatcag catcgaagct gaataagttt
    28261 ttcgcgtccc ggtaacggat gaaagccccg caacacgttg cggggctttt tacattcgat
    28321 aaccggtaaa aatgcgcatg gtgcgacgtt aatccgcaaa tgcgctttgc agggaatacg
    28381 gtatgaagca gcaggcaggc attggcattc ttttggcgct taccacagca atttgctggg
    28441 gggcgttgcc aatcgcaatg aagcaggtgc tggaggtgat ggaacctccg acaatcgtgt
    28501 tttaccgttt cttgatggcg agtattggcc ttggtgccat tcttgcggtg aagaagaggt
    28561 tgccgccatt acgcgtgttt cgtaagccac gctggttgat tttgttggca gtggcgaccg
    28621 ccgggctgtt tgggaacttc atcctgttca gctcatcctt gcaatacctg agtccgaccg
    28681 cttcgcaggt gattgggcaa ctctcgccag ttggcatgat ggttgccagc gtatttatcc
    28741 tgaaagagaa aatgcgcagc actcaggttg taggggcatt gatgctcctg agcggtctgg
    28801 tgatgttttt taacaccagt ctggtcgaga tatttacaaa gctcaccgat tacacctggg
    28861 gagttatctt tggggtcggt gcggcgacgg tttgggtgag ttatggcgtg gcgcaaaagg
    28921 ttttattgcg tcggctggcc tcaccgcaga tcctgttttt actgtacact ttatgtacaa
    28981 tcgcgctctt ccctctggca aagcctggag tgatagcgca gcttagccac tggcagctcg
    29041 catgtttaat tttttgcgga ctgaatacct tggtaggata tggcgccttg gcggaagcga
    29101 tggctcgctg gcaggcagcg caggtgagcg cgatcatcac gctcacccca ctgtttacgc
    29161 tgtttttttc agatctttta tcactggcct ggcccgattt cttcgccaga ccgatgttaa
    29221 accttttagg ttatctcggt gcgtttgtcg tggttgcggg cgcgatgtat tccgccattg
    29281 gtcatcgtat ttggggcgga ttacgtaagc atacaacggt ggtatcgcaa ccccgcgcag
    29341 gcgaatgatt tacggagaat aaaatgaagt ttgttgatga agcatcgatt ctggtcgttg
    29401 caggtgatgg cggtaatggt tgcgtgagct tccgccgcga aaagtatatt ccgaaaggcg
    29461 gcccggatgg cggcgacggc ggtgatggtg gtgacgtatg gatggaagcc gacgagaacc
    29521 tgaacacgct tatcgattat cgttttgaaa aatctttccg tgcagagcgc ggtcagaacg
    29581 gcgcaagccg cgattgtacc ggtaagcgcg gtaaagacgt gacgattaaa gtgccggtag
    29641 gtacgcgtgt aatcgaccag ggtactggtg aaaccatggg tgatatgact aaacacggtc
    29701 agcgtctgct ggttgctaag ggcggctggc acggtctggg caatacccgt ttcaaatcgt
    29761 cagttaaccg tacaccacgg cagaaaacta acggtacgcc gggcgataag cgtgagctgc
    29821 tgctggagct gatgctgctg gctgacgtcg gtatgttggg gatgccaaac gcgggtaaat
    29881 cgacctttat tcgtgcggta tcggcggcta aaccgaaagt ggcggattat ccgtttacca
    29941 ctctggtgcc aagtctgggt gtggtacgaa tggacaacga aaagagcttc gttgttgccg
    30001 atattccagg actgattgaa ggcgctgcgg aaggcgcagg tctgggcatt cgcttcctga
    30061 agcacctgga acgttgccgc gtcctgttgc acctcatcga tatcgatccg attgacggca
    30121 ccgatccggt tgaaaacgcg cgtattatta tcagcgagct ggaaaaatac agccaggatc
    30181 tggcggcgaa accgcgttgg ttagtcttca acaagatcga tctgctggat aaggcagaag
    30241 ccgaagagaa agcgaaagcg atcgctgaag cgctgggctg ggaagataaa tattatctga
    30301 tctctgcggc gagcggactg ggcgtgaaag atctctgctg ggatgtgatg acctttatca
    30361 ttgaaaaccc ggtcgtgcag gctgaagaag cgaaacagcc agagaaagtc gaattcatgt
    30421 gggatgatta tcaccgtcag cagcttgaag agattgctga agaggatgat gaagactggg
    30481 atgacgactg ggacgaagac gacgaagaag gcgttgagtt catttacaag cgttaatcac
    30541 taatgaccaa tgccaaataa gcctgatgcg ctacacttat caggcctaca agatgattgc
    30601 aatatattga atttgcacga ttttgtaggc cggataaggc gttcacgccg catccggcat
    30661 aaacaaagcg cactttgtca ataatctgaa accccggcca tgcgccgggg tttctttttg
    30721 actaattgtt ctgataaata tctttgtaca aacggctttc aaaacgcact aacggaatac
    30781 ggcgattacg ctgatccgcg ggttttactg catagccaga aagatattgt acaaatgcca
    30841 ttcgttgccc gctcgctgtg gtaatgaatc ccgccaggtt atataccccc tgcaacgaac
    30901 cggttttcgc tgagactttg ccatccacgc ccgcctgatg cagacctgca cggtactgca
    30961 aagagccgtc atagcccgca agcggcagca tggagataaa gttaagttca ttgtcgtgtt
    31021 gggcaatgta ttgcagcacc tgcatcatgg tggcgggggc aatcaggtta tgccgcgaaa
    31081 gccctgaacc atcggcaata atggtgttac caatatcgac accggcttgc tggcgcagga
    31141 tctgacgcac ggcgtccgac ccagcccgcc atgttccagg cacattgaag cgcgcatggc
    31201 cgatcatgcg gaaaaccgta tcggcgatca tgttgtccga ctttttcagc ataatcttaa
    31261 gcagatcgtg cagcggggcc gactgtttac tggcaactac cgttccaggt tcgttaacct
    31321 gagtctggcg cagcagtgtt ccgctccagg tgatacccgc ctgttttaac tcatctttca
    31381 gaattgcacc ggcatagctg gctccatcct gcacggcaaa agccaacggg agcggctcag
    31441 aacgttgtgg caggcatccc gtcagcgtaa agcggttcag gtcgcttggc accacatcca
    31501 gttcgcagta ttgcgcttcg gcagaaccac gggggagggt gcgtacctgg ctgaacatcg
    31561 taacggggta ataagatgcc acgcgtataa aagccatatc accaggcttt ggggcactgt
    31621 agagcgagac ggagaaacag ttgcggtcaa ctatggcggc agcaggcgga gcgctaaagc
    31681 attgtgtcat gtcattccat ggccagccgg gggctttatc gtggctggcg aaaatggagg
    31741 tatctatcaa cacattgcca tcgatttggt tgacgccaga ttttttcaaa gtcgcgacca
    31801 tattgcgaat atcctgacgt tttaacgtcg gatcggcacc aaatcgcgcc actaagtcac
    31861 ccttaagtac gccgttttcc acattgcctt tggtttcaag cgtcgtggtg aaacgaaaat
    31921 cggggccgag ttgaatcaac gccgccagcg cagtaatcac tttctgggta ctggcgggca
    31981 gcgccatctg ctgactgtgg taatcaatag cgggggccga cgcaccgact ttttgcacca
    32041 tcagggcaag gttggcacct gcggggagtt gagtaacgta ctcatcaaca ttcgcggcct
    32101 gaacactgaa cgctatacag ctggtcaatc cgatgataaa tctggaaaat cgcataatct
    32161 cgcgctaaca acctggaatc gagccgtcat actacggcgc aacgccctat aaagtaaacg
    32221 atgacccttc gggaacttca gggtaaaatg accatcaaaa tgtgaattgt agctgacctg
    32281 ggacttgtac ccgggtcggt atttttttgc ttctggtccc ggtgaggagt tatgccgggc
    32341 aggccgaaca gccggggtgg gtgaagactt gccccatcag gaatattcaa gaggtataac
    32401 aaatgcaagc tattccgatg accttacgcg gcgctgaaaa attacgcgaa gagctggatt
    32461 ttctgaaatc tgtgcgccgt cctgaaatca ttgctgctat cgcggaagcg cgtgagcatg
    32521 gcgacctgaa agaaaacgcc gaataccacg cagctcgtga acagcagggc ttctgcgaag
    32581 gccgtatcaa agacatcgaa gccaagctgt caaacgcgca ggtgattgat gtcaccaaaa
    32641 tgcccaacaa tgggcgcgtt atttttggtg ctaccgtaac ggtgctgaat ctggattctg
    32701 acgaagaaca gacttatcgc atcgttggcg atgacgaagc tgactttaaa caaaacctga
    32761 tttctgtaaa ctcgcctatt gctcgtggcc tgatcggcaa agaagaagat gatgttgtgg
    32821 tcatcaaaac gccgggcggc gaagtagaat ttgaagtaat taaggtggaa tacctgtaag
    32881 aattacccaa tactcaagat gttgatgtat tgtaaagaaa ggaaaaaggc cgctctgcgg
    32941 ccttttatca acgaacagag cgtggcattt tgctctcctg cctgcggaaa acccctcgtt
    33001 ttacacagca aatgtgtgta actttaggat aatcttagcg tggcagcgag attttacgtt
    33061 ctttagttgg gcgataaagc accagcgttt taccgatgac ctgtacatta caggcgccgg
    33121 tttcgcgcac gatagcttcc acgatcaagg tttttgtttc gcgatcttcg gtggcgattt
    33181 tcaccttgat gagttcatgg tgctctaacg cttgttcaat ctcggccagc accccttcgg
    33241 tcaaaccatt actgccaagc agaacaactg gcttgagcgg atgtgccaga cctttcaggt
    33301 gctgtttttg tttagtactc agattcatcg tattttttgc ttacgttggg attgaaaacg
    33361 ggtcattcta ccgccatctc ccatatatca ccaaataggc gcgtaaaaat ttacgcaatt
    33421 ggttacgatg agttatcccc atgggaaagt taaatgacag gtaagaagcg ttctgccagc
    33481 tccagccgct ggcttcagga acactttagc gataaatatg ttcaacaggc acagaaaaag
    33541 ggattacgtt cccgtgcctg gtttaaactt gatgaaatac agcaaagtga caaactcttt
    33601 aaaccgggaa tgacggttgt cgaccttggt gctgctccgg gtggttggtc acaatatgtg
    33661 gtcacccaaa ttggcggcaa aggccgcatc atcgcttgcg atcttttacc tatggatcct
    33721 atcgttggtg tggactttct tcagggcgat tttcgtgatg aactggtgat gaaagcactg
    33781 ctggagcgcg ttggcgacag caaagtccag gttgtcatgt ccgatatggc accaaacatg
    33841 agcggaacac cggcggtgga tatcccccga gccatgtatc tggtggaact ggcgctagaa
    33901 atgtgtcgtg atgtattagc gccaggtggc agttttgtag tgaaggtgtt ccagggcgaa
    33961 ggtttcgatg agtatctaag ggaaattcgc tccctgttta cgaaggtcaa agttcgtaag
    34021 ccggactctt ctcgtgcacg ttcgcgagaa gtgtatattg tagcgaccgg gcgtaaaccc
    34081 taaccgggag atttcagacg aaagtttgaa agatgctgga tatagagtat cctgacgctg
    34141 tttttaacac agttgtaata agaggttaat cccttgagtg acatggcgaa aaacctaata
    34201 ctctggctgg tcattgccgt tgtgctgatg tcagtattcc agagctttgg gcccagcgag
    34261 tctaatggcc gtaaggtgga ttactctacc ttcctacaag aggtcaataa cgaccaggtt
    34321 cgtgaagcgc gtatcaacgg acgtgaaatc aacgttacca agaaagatag taaccgttat
    34381 accacttaca ttccggttca ggatccgaaa ttactggata acctgttgac caagaacgtc
    34441 aaggttgtcg gtgaaccgcc tgaagaacca agcctgctgg cttctatctt catctcctgg
    34501 ttcccgatgc tgttgctgat tggtgtctgg atcttcttca tgcgtcaaat gcagggcggc
    34561 ggtggcaaag gtgccatgtc gtttggtaag agcaaagcgc gcatgctgac ggaagatcag
    34621 atcaaaacga cctttgctga cgttgcgggc tgcgacgaag caaaagaaga agttgctgaa
    34681 ctggtagagt atctgcgcga gccgagccgc ttccagaaac tcggcggtaa gatcccgaaa
    34741 ggcgtcctga tggtcggtcc tccgggtacc ggtaaaacgt tgctggcgaa agcgattgca
    34801 ggcgaagcga aagttccgtt ctttactatc tccggttctg acttcgtaga aatgttcgtc
    34861 ggtgtgggtg catcccgtgt tcgtgacatg ttcgaacagg cgaagaaagc ggcaccgtgc
    34921 atcatcttta tcgatgaaat cgacgccgta ggccgccagc gtggcgctgg tctgggcggt
    34981 ggtcacgatg aacgtgaaca gactctgaac cagatgctgg ttgagatgga tggcttcgaa
    35041 ggtaacgaag gtatcatcgt tatcgccgcg actaaccgtc cggacgttct cgacccggcc
    35101 ctgctgcgtc ctggccgttt cgaccgccag gttgtggtcg gcttgccaga tgttcgcggt
    35161 cgtgagcaga tcctgaaagt tcacatgcgt cgcgtaccat tggcacccga tatcgacgcg
    35221 gcaatcattg ctcgtggtac tcctggtttc tccggtgctg acctggcgaa cttggtgaac
    35281 gaagcggcac tgttcgctgc tcgtggcaac aaacgcgttg tgtcgatggt tgagttcgag
    35341 aaagcgaaag acaaaatcat gatgggtgcg gaacgtcgct ccatggtgat gacggaagcg
    35401 cagaaagaat caacggctta ccacgaagcg ggtcatgcga ttatcggtcg cctggtgccg
    35461 gaacacgatc cggtgcataa agtgacgatt atcccacgcg gtcgtgcgct gggtgtgact
    35521 ttcttcttgc ctgagggcga cgcaatcagc gccagccgtc agaaactgga aagccagatt
    35581 tctacgctgt acggtggtcg tctggcagaa gagatcatct acgggccgga acatgtttct
    35641 accggtgcgt ccaacgatat taaagttgcg accaacctgg cacgtaacat ggtgactcag
    35701 tggggcttct ctgagaaatt gggtccactg ctgtacgcgg aagaagaagg tgaagtattc
    35761 ctcggccgta gcgtagcgaa agcgaaacat atgtccgatg aaactgcacg tatcatcgac
    35821 caggaagtga aagcactgat tgagcgtaac tataatcgtg cgcgtcagct tctgaccgac
    35881 aatatggata ttctgcatgc gatgaaagat gctctcatga aatatgagac tatcgacgca
    35941 ccgcagattg atgacctgat ggcacgtcgc gatgtacgtc cgccagcggg ctgggaagaa
    36001 ccaggcgctt ctaacaattc tggcgacaat ggtagtccaa aggctcctcg tccggttgat
    36061 gaaccgcgta cgccgaaccc gggtaacacc atgtcagagc agttaggcga caagtaagtt
    36121 cccgcatcag atgactgtat ttgtaccgaa aaccccgggg cgtgctccgg ggttttttct
    36181 tatcaattca taccagggat aacataatga aactctttgc ccagggtact tcactggacc
    36241 ttagccatcc tcacgtaatg gggatcctca acgtcacgcc tgattccttt tcggatggtg
    36301 gcacgcataa ctcgctgata gatgcggtga aacatgcgaa tctgatgatc aatgctggcg
    36361 cgacgatcat tgacgttggt ggcgagtcca cgcgcccagg ggcggcggaa gttagcgttg
    36421 aagaagagtt gcaacgtgtt attcctgtgg ttgaggcaat tgctcaacgc ttcgaagtct
    36481 ggatctcggt cgatacatcc aaaccagaag tcatccgtga gtcagcgaaa gttggcgctc
    36541 acattattaa tgatatccgc tccctttccg aacctggcgc tctggaggcg gctgcagaaa
    36601 ccggtttacc ggtttgtctg atgcatatgc agggaaatcc aaaaaccatg caggaagctc
    36661 cgaagtatga cgatgtcttt gcagaagtga atcgctactt tattgagcaa atagcacgtt
    36721 gcgagcaggc gggtatcgca aaagagaaat tgttgctcga ccccggattc ggtttcggta
    36781 aaaatctctc ccataactat tcattactgg cgcgcctggc tgaatttcac catttcaacc
    36841 tgccgctgtt ggtgggtatg tcacgaaaat cgatgattgg gcagctgctg aacgtggggc
    36901 cgtccgagcg cctgagcggt agtctggcct gtgcggtcat tgccgcaatg caaggcgcgc
    36961 acatcattcg tgttcatgac gtcaaagaaa ccgtagaagc gatgcgggtg gtggaagcca
    37021 ctctgtctgc aaaggaaaac aaacgctatg agtaatcgta aatatttcgg taccgatggg
    37081 attcgtggtc gtgtagggga tgcgccgatc acacctgatt ttgtgcttaa gctgggttgg
    37141 gccgcgggta aagtgctggc gcgccacggc tcccgtaaga ttattattgg taaagacacg
    37201 cgtatttctg gctatatgct ggagtcagca ctggaagcgg gtctggcggc agcgggcctt
    37261 tccgcactct tcactggccc gatgccaaca ccggccgtgg cttatctgac gcgtaccttc
    37321 cgcgcagagg ccggaattgt gatatctgca tcgcataacc cgttctacga taatgggatt
    37381 aaattcttct ctatcgacgg cactaaactg ccggatgcgg tagaagaggc catcgaagcg
    37441 gaaatggaaa aggagatcag ctgcgttgat tcggctgaac tgggtaaagc cagccgtatc
    37501 gttgatgccg cgggtcgcta tatcgagttt tgcaaagcca cgttcccgaa cgaacttagc
    37561 ctcagtgaac tgaagattgt ggtggattgt gcaaacggtg cgacttatca catcgcgccg
    37621 aacgtgctgc gcgaactggg ggcgaacgtt atcgctatcg gttgtgagcc aaacggtgta
    37681 aacatcaatg ccgaagtggg ggctaccgac gttcgcgcgc tccaggctcg tgtgctggct
    37741 gaaaaagcgg atctcggtat tgccttcgac ggcgacggcg atcgcgtgat tatggttgac
    37801 catgaaggca ataaagtcga tggcgatcag atcatgtata tcatcgcgcg tgaaggtctt
    37861 cgtcagggcc agctgcgtgg tggtgctgtg ggtacattga tgagcaacat ggggcttgaa
    37921 ctggcgctga aacagttagg aattccattt gcgcgcgcga aagtgggtga ccgctacgta
    37981 ctggaaaaaa tgcaggagaa aggctggcgt atcggtgcag agaattccgg tcatgtgatc
    38041 ctgctggata aaaccactac cggtgacggc atcgttgctg gcttgcaggt gctggcggcg
    38101 atggcacgta accatatgag cctgcacgac ctttgcagcg gcatgaaaat gttcccgcag
    38161 attctggtta acgtacgtta caccgcaggt agcggcgatc cgcttgagca tgagtcagtt
    38221 aaagccgtga ccgcagaggt tgaagctgcg ctgggcaacc gtggacgcgt gttgctgcgt
    38281 aaatccggca ccgaaccgtt aattcgcgtg atggtggaag gcgaagacga agcgcaggtg
    38341 actgaatttg cacaccgcat cgccgatgca gtaaaagccg tttaaagcgt tagataactg
    38401 gctaaaaagg cggcgattgt cgcctttttt ctcggcgaac acatcggttt tgctgttttt
    38461 ttccgcagtt gatacaatgc gataaaattg cccttgcgaa ggtcattcgt tttggttagt
    38521 attcacaccc gcttcagtgg gaaagattaa aaactcccgc tttattggtt gaagcattgg
    38581 tacgcggcaa ctccgcaagg aacaggttga ttatgtacga agctctttta gtagttttcc
    38641 ttattgtggc aattggcctt gttggtctga tcatgctgca gcaaggtaaa ggcgctgata
    38701 tgggagcctc cttcggagca ggcgcttccg ctacgctgtt tggttcaagt ggttctggta
    38761 acttcatgac ccgcatgacg gcgctgctgg caacgttatt cttcatcatc agtctggtgc
    38821 tgggtaacat caatagcaac aaaaccaata gaggtagcga atgggaaaat ctgagtgcac
    38881 cggcgaaaac cgaacaaact cagccagctg ctccggctaa gccgaccagc gatatcccga
    38941 actaaaaagt agtatccgtg ccgaggtggt ggaattggta gacacgctac cttgaggtgg
    39001 tagtgcccaa tagggcttac gggttcaagt cccgtcctcg gtaccaaatt ccagaaaaga
    39061 gacgctgaaa agcgtctctt ttcgttttgg tcctggtctg atgaatggtt gtcaggtatc
    39121 tgtatctcgt ccagttgctt tactcgtagc tggtaccttg aaacgatggt gccggtacgc
    39181 cttagttata aacttttccc tactgctgac ataattggca ctttattgtg tcaactccag
    39241 ttccggtacg aatacggata tgtttacttc tcgataaacc atcctgctcc tgtcagtata
    39301 actactgatg gctacgttct gcgtaatgtt tagagacgca ttgataatgt tatttaatta
    39361 ctgagaacgc aatggtcgca tcatttaaaa agaaaaaact aacagtgttt cggtctgata
    39421 tggatcagag ttgaaaaaca tgtctgatat aaaattgaac attgttgata attgtttata
    39481 ttggttgtga acttggattt ctggatgaca gtattcaaca aatttgctag aagattttaa
    39541 atctcattgg ttgttgtatc tttgtgttat tgtttttggt attacgaact tagtcgcctc
    39601 ttccggagcg catatggttc agcgattgct gttcttcgtc ctgaccatcc tggttgttaa
    39661 acgtatatca tcccttccgc ttcgcctgct tgttgccgca ccatttgtgt tactgactgc
    39721 ggcagacatg agtattagcc tctattcatg gtgtaccttt ggtacaactt tcaatgatgg
    39781 atttgcgatt agtgtgctgc agagtgatcc ggatgaagtt gtgaaaatgc tggggatgta
    39841 tatcccttat ctatgtgctt ttgctttttt atcccttctt tttttggcag taataataaa
    39901 atatgatgtt tccttgccga caaaaaaagt gacaggaata ttattgctga ttgtcatttc
    39961 gggcagttta ttttccgctt gtcaatttgc ttataaagat gcaaaaaata aaaaagcgtt
    40021 cagtccatat atactagcgt cgtgatttgc tacctatacg ccgtttttca atctcaacta
    40081 ttttgcttta gcagcgaaag cgcatcaaag attactctca attgcaaaca cggtgccgta
    40141 ttttcaatta tcagtcaggg atacaagtat tgatacctac gtgttgattg tgggggagtc
    40201 tgtacgtgtc gacaatatgt ctttgtatgg atatacacgc tctacgacac cgcaagttga
    40261 agcacaaaga aaacagatca aactgtttaa tcaagcaata agcggcgcac cttacactgc
    40321 gctgtcggtt cccctttctt taactgctga ttctgttttg agtcatgaca ttcataatta
    40381 ccccgacaac attattaata tggctaatca agcaggattt cagactttct ggctaagctc
    40441 gcaatccgct tttcggcaga atggtacagc agttaccagt atcgccatgc gcgccatgga
    40501 aacagtttat gtcagaggat ttgatgaatt gttgttgccg catttatcgc aagcattaca
    40561 gcaaaatacg cagcaaaaga aactgattgt tcttcattta aatggaagcc atgaaccggc
    40621 ttgtagcgcc tatccgcaat ccagcgccgt gtttcaaccg caggacgatc aggatgcctg
    40681 ctatgacaac tccattcatt acacagatag tttgctaggt caggtttttg aattattaaa
    40741 agatcgccgc gcctcggtca tgtattttgc cgaccacggc ctggaacgtg accctacgaa
    40801 gaagaacgtc tattttcatg gaggcaggga ggctagccag caggcatatc atgtcccgat
    40861 gtttatctgg tatagccccg ttcttgggga tggcgtggat cgcacaacgg aaaacaatat
    40921 cttttcgaca gcttacaata attaccttat taatgcgtgg atgggggtaa caaagccgga
    40981 acagccgcaa acgcttgagg aagtgattgc acactataaa ggagactcac gggttgtgga
    41041 tgcaaaccat gatgttttcg attatgtgat gctcagaaag gagtttacag aggataagca
    41101 aggtaacccc acccctgaag ggcagggttg atgtcgaatt actggccttt gttttccata
    41161 ttctccacct gcggcaggcc tgaagtggca gaggaggaaa gcaggccagt tttggcataa
    41221 ccaaaaagtt tctctcgggt atcagtgata tccagattac gcatggtcaa ttgaccaata
    41281 cgatcatctg gcgagaacac cgagtcgcct ttttccatcg tcagacgctc tggcttgtag
    41341 gtcaggttct ctgagacggt attcaggatt gaataatcgt tcccacggcg cagctccagg
    41401 gtaacttcac cagtgatctg gctggcaacc cagcgttgca gagagtcacg cagcatcagc
    41461 gcctgggagt caaaccaacg cccctggtac agcagacggc ccaactgacg accatgcgcg
    41521 tgatactgct caatggtgtc ttcgttgtga ataccggtca acagacgttc atacgcaatg
    41581 tgcagcagtg ccatccccgg agcttcgtaa ataccacggc ttttcgcttc gatgatacgg
    41641 ttctcaatct ggtcgctcat gcccaggccg tgacgaccgc cgatgcggtt agcttccagc
    41701 atcatttcta cgtcgtcgct aaaggtttta ccgttcagcg ccaccggatg accttgctca
    41761 aagcgtactg tgacttcttc tgccgggatc ttcacgctct catcccagaa tttcacgccc
    41821 ataatcgggt tgacgatttt gacgctggag ttgaggtatt ccagatcctt cgcttcatgc
    41881 gttgcaccaa gcatgttgga gtctgtggag taggcttttt cgacagacat tttgtagtcg
    41941 aaaccgcagg caatcataaa ttcagacatc tcatgacggc cgcccagttc atcaataaag
    42001 tcagtatcaa gccacggttt gtaaatctgc agttcagcat tggtcagcag accataacga
    42061 tagaaacgtt cgatatcgtt tcctttatag gtgctaccgt caccccagat attcacgcca
    42121 tcttctttca tcgcagcaac cagcatggta ccagtcacgg cgcggcccag cggcgtcgtg
    42181 ttgaaatagg tcaggccgcc ggtggtgtta tgaaatgcgc cacactgaat agcggcaata
    42241 ccttcggcca ccagttgttt gcggcagtcg atcagacgtg cgttctccgc gccgtattcc
    42301 atggcacgac gagggatcgc atcataatcc tcttcgtctg gctggcccag gtttgcagta
    42361 tatgcataag gaaccgctcc cttttgtcgc atccacagca gtgcggcact ggtgtccaga
    42421 ccgccggaaa aagcgatacc aatacgttga cctaccggga gatgcttgag aatcgtcgtc
    42481 ataaaataac accctgctta attaactgat gatgagcctg gatttccgct ctcactgaat
    42541 ttttatgcaa aataaatgag ttttcattta atcatctttt atcggagaca ggaagagttt
    42601 agtgtgtttt ttgtaaaata atgcgcttaa gggagagcag gggaaggcaa aagtattcaa
    42661 caaatgaaag tgaactggat attcattcac gtgattagca ataaacgttg acaaaatgtg
    42721 gcatggatca ctataatgcc tgcagatttt acgtcccgtc tcggtacacc aaatcccagc
    42781 agtatttgca ttttttaccc aaaacgagta gaatttgcca cgtttcaggc gcggggtgga
    42841 gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat ccggcccccg
    42901 caaccacttt cccttagagt cctttttcaa atatactgtg aagacttcgg ccttcgtagt
    42961 gggatttgaa aaaatccttc tggaaagtgc tccagaccgc agttgcggtt atagggttca
    43021 gttatataaa gccccgattt atcggggttt tttgttatct gactacagaa taactgggct
    43081 ttaggccctt tttttatgtc ttgggggtgg gcttgtccac attagagcaa aaattaacag
    43141 agatgattac tgcgccagtt gaggccctgg gttttgaact ggtaggcatc gaatttattc
    43201 gcggtcgcac atccacactg cgcatctata ttgatagtga agatggcatc aatgttgatg
    43261 attgtgctga tgtgagccac caggtaagtg ctgtgctgga tgttgaagat cccatcaccg
    43321 ttgcttataa cctggaagtc tcctcaccgg gtctcgatcg cccactgttc acggctgaac
    43381 actacgcccg ttttgtcgga gaagaggtga ctctggttct ccgtatggcg gtacaaaacc
    43441 gtcgtaaatg gcagggcgtt atcaaagcgg tagacggtga aatgatcaca gttaccgtcg
    43501 aaggtaaaga tgaagtgttc gcgctgagta atatccagaa ggcgaacctg gttccccact
    43561 tttaatagtc tggatgaggt gaaaagcccg cgatgaacaa agaaattttg gctgtagttg
    43621 aagccgtatc caatgaaaag gcgctacctc gcgagaagat tttcgaagca ttggaaagcg
    43681 cgctggcgac agcaacaaag aaaaaatatg agcaagagat cgacgtccgt gtacagatcg
    43741 atcgcaaaag cggtgatttt gacaccttcc gtcgctggtt agttgttgat gaagtcaccc
    43801 agccgaccaa ggaaatcacc cttgaagccg cacgttatga agatgaaagc ctgaacttgg
    43861 gcgattacgt tgaagatcag attgagtctg ttacctttga ccgtatcacc acccagacgg
    43921 caaaacaggt tatcgtgcag aaagtgcgtg aagccgaacg tgcgatggtg gttgatcagt
    43981 tccgtgaaca cgaaggtgaa atcatcaccg gcgtggtgaa aaaagtaaac cgcgacaaca
    44041 tctctctgga tctgggcaac aacgctgaag ccgtgatcct gcgcgaagat atgctgccgc
    44101 gtgaaaactt ccgccctggc gaccgcgttc gtggcgtgct ttattccgtt cgcccggaag
    44161 cgcgtggcgc gcaactgttc gtcactcgtt ccaagccgga aatgctgatc gaactgttcc
    44221 gtattgaagt gccggaaatc ggcgaagaag tgattgaaat taaagcagcg gctcgcgacc
    44281 cgggttctcg tgcgaaaatc gcggtgaaaa ccaacgataa acgtatcgat ccggtaggtg
    44341 cttgcgtagg tatgcgtggc gcgcgtgttc aggcggtttc tgctgaactg ggcggcgagc
    44401 gtatcgatat cgtcctgtgg gatgataacc cggcgcagtt tgtgattaac gcaatggcac
    44461 cggcagacgt tgcttctatc gtggtggatg aagataaaca caccatggat atcgccgttg
    44521 aagctggtaa cctggcgcag gcgattggcc gtaacggtca gaacgtgcgt ctggcttcgc
    44581 agctgagcgg ttgggaactc aacgtaatga ccgttgacga cctgcaggct aagcatcagg
    44641 cggaagcgca cgcagcgatc gacaccttca ccaaatatct cgacatcgac gaagacttcg
    44701 cgactgttct ggtagaagaa ggtttctcga cgctggaaga actggcctat gtgccgatga
    44761 aagagctgtt ggaaatcgaa ggccttgatg agccgaccgt tgaagcactg cgcgagcgtg
    44821 ctaaaaatgc actggccacc attgcacagg cccaggaaga aagcctcggt gataacaaac
    44881 cggctgacga tctgctgaac cttgaagggg tagatcgtga tttggcattc aaactggccg
    44941 cccgtggcgt ttgtacgctg gaagatctcg ccgaacaggg cattgatgat ctggctgata
    45001 tcgaagggtt gaccgacgaa aaagccggag cactgattat ggctgcccgt aatatttgct
    45061 ggttcggtga cgaagcgtaa taaactgtag caggaaggaa cagcatgaca gatgtaacga
    45121 ttaaaacgct ggccgcagag cgacagacct ccgtggaacg cctggtacag caatttgctg
    45181 atgcaggtat ccggaagtct gctgacgact ctgtgtctgc acaagagaaa cagactttga
    45241 ttgaccacct gaatcagaaa aattcaggcc cggacaaatt gacgctgcaa cgtaaaacac
    45301 gcagcaccct taacattcct ggtaccggtg gaaaaagcaa atcggtacaa atcgaagtcc
    45361 gcaagaaacg cacctttgtg aaacgcgatc cgcaagaggc tgaacgcctt gcagcggaag
    45421 agcaagcccg tcgtgaggca gaagaatcgg ctaaacgcga ggcgcaacaa aaagctgaac
    45481 gtgaggccgc agaacaagct aagcgtgaag ctgctgaaca agcgaaacgt gaagctgcgg
    45541 aaaaagacaa agtgagcaat caacaagacg atatgactaa aaacgcccag gctgaaaaag
    45601 cccgccgtga gcaggaagct gcagagctca agcgtaaagc tgaagaagaa gcgcgtcgta
    45661 aactcgaaga agaagcacgt cgcgttgctg aagaagcacg tcgtatggcg gaagaaaaca
    45721 aatggactga taacgcggaa ccgactgaag attccagcga ttatcacgtc actacttctc
    45781 aacatgctcg ccaggcagaa gacgaaagcg atcgtgaagt cgaaggcggc cgtggccgtg
    45841 gtcgtaacgc gaaagcagcg cgtccgaaga aaggcaacaa acacgctgaa tcaaaagctg
    45901 atcgtgaaga agcacgcgca gcagtacgtg gcggtaaagg cggaaaacgt aaaggttctt
    45961 cgctgcagca aggcttccag aagcctgctc aggccgttaa ccgtgacgtt gtgatcggcg
    46021 aaactatcac cgttggcgaa ctggcgaaca agatggcggt taaaggctct caggtcatca
    46081 aagcgatgat gaaactgggc gcaatggcaa ccatcaacca ggttatcgat caggaaaccg
    46141 cacagctggt tgctgaagag atgggccata aagttatcct gcgtcgtgaa aacgagctgg
    46201 aagaggcggt aatgagcgac cgtgacacgg gtgctgcggc tgaaccgcgc gcgccggttg
    46261 tgaccatcat gggtcacgtt gaccacggta aaacctctct gctggactac attcgttcaa
    46321 cgaaagtggc ctctggcgaa gcgggcggca ttacccagca cattggtgca taccacgttg
    46381 aaactgaaaa cggcatgatc accttcctgg acaccccggg gcacgccgcg tttacttcaa
    46441 tgcgtgctcg tggtgcgcag gcaacggaca tcgtagtcct ggttgttgct gccgacgacg
    46501 gcgtaatgcc gcagacgatc gaagcaatcc agcacgcgaa agcggcgcag gttccggtgg
    46561 tcgttgcagt gaacaagatc gataaaccag aagccgatcc ggatcgcgtt aagaacgaac
    46621 tctcccagta cggcatcctg ccggaagagt ggggcggtga aagccagttc gtacatgtgt
    46681 ctgcgaaagc gggtactggt atcgatgaac tgctggacgc tatcctgctg caggcggaag
    46741 ttctggagct gaaagcggta cgtaaaggta tggcgagcgg tgcggttatc gaatccttcc
    46801 tcgataaagg tcgtggtccg gttgctaccg ttctggtacg tgaaggtact ctgcacaagg
    46861 gcgatatcgt tctgtgtggc ttcgaatacg gtcgtgttcg tgcgatgcgt aacgaactgg
    46921 gtcaggaagt gctggaagcg ggtccgtcca ttccggtgga aatcctcggc ctgtccggcg
    46981 taccggctgc gggtgatgaa gttaccgttg tacgtgacga gaagaaagcg cgtgaagttg
    47041 cactgtatcg tcagggtaaa ttccgcgaag ttaaactggc gcgtcagcag aaatctaaac
    47101 tcgagaacat gttcgccaac atgaccgaag gcgaagttca cgaagtgaac atcgtcctga
    47161 aggcagacgt acagggttct gtggaagcga tctccgactc cttgctgaaa ctgtctactg
    47221 acgaagttaa agtgaagatc atcggttctg gcgtaggtgg tatcaccgaa accgacgcca
    47281 ccctggctgc ggcgtccaac gccatcctgg ttggcttcaa cgtacgtgct gatgcctctg
    47341 cacgtaaagt gattgaagcg gaaagcctgg atctgcgtta ctactccgtc atctataacc
    47401 tgattgacga agtgaaagcg gcgatgagcg gtatgctgtc tccggaactg aaacagcaga
    47461 ttatcggtct ggcggaagtt cgtgacgtgt tcaaatcgcc gaaatttggt gccatcgcag
    47521 gctgtatggt taccgaaggt gtggttaaac gtcacaaccc gatccgcgtt ctgcgtgaca
    47581 acgtggttat ctacgaaggc gagctggagt ccctgcgccg cttcaaagat gacgttaacg
    47641 aagtccgtaa cggtatggaa tgtggtatcg gcgttaagaa ctacaacgac gtccgcactg
    47701 gcgatgtgat cgaagtattc gaaatcatcg agatccaacg taccatcgct taaggttttt
    47761 agggttccat gcgggataag gcgtttacgc cgcatccggc atggattgcc tgatgtgacg
    47821 ctggcgcgtc ttatcaggcc aacgtgaaca gcccttccac ctgaaattaa ttttaaaaag
    47881 gggctaacag cccctttttt gtcaggagaa tttattatgg cgaaagaatt tggtcgcccg
    47941 cagcgcgtag cgcaggaaat gcaaaaagag atcgctctca tcctgcagcg tgaaattaaa
    48001 gatcctcgcc tgggcatgat gaccaccgtt tccggtgtcg aaatgtctcg cgacctggcg
    48061 tatgccaaag tatatgtgac gttcctcaac gacaaagatg aagacgcggt taaagcgggc
    48121 atcaaagcgt tgcaagaagc ttctggtttc atccgcagcc tgctggggaa agcgatgcgc
    48181 ctgcgtatcg tgccggaact gaccttcttc tacgacaact ctctggttga agggatgcgc
    48241 atgtcaaacc tggtgaccag cgtggtcaaa catgacgaag aacgtcgtgt taacccggac
    48301 gacagcaagg aggactaatg agtcgtcctc gtcgtcgcgg tcgcgacatt aacggcgttt
    48361 tgttgctgga taaaccgcag ggtatgtcca gcaatgatgc gctgcaaaaa gtgaaacgga
    48421 tttataacgc caaccgtgcc gggcataccg gtgcgctgga cccgctggca actggcatgt
    48481 tgccaatttg cctcggggaa gcgacgaagt tttcccagta tctgctggac tccgacaaac
    48541 gctatcgggt cattgcgcgt cttggacagc gtaccgatac ttctgatgcc gacggacaaa
    48601 tcgttgaaga acgtccggta acctttagcg cagagcaact ggcggcggcg ctggatactt
    48661 tccgtggcga tatcgaacag atcccttcga tgtattcagc actgaaatat cagggtaaaa
    48721 aactgtacga atatgcgcgt cagggcattg aagtaccgcg tgaagcgcgc ccgattaccg
    48781 tttatgaatt gctgtttatt cgccacgaag gcaatgagct ggagctggaa attcactgct
    48841 caaaaggcac ttatatccgc accatcattg atgacctggg tgaaaaactc ggttgtggcg
    48901 cgcatgttat ttacctgcgt cgtctggcgg taagtaaata tccggttgaa cggattgtga
    48961 ccctggaaca tctgcgtgaa ctggtcgaac aagccgaaca gcaggatatt ccggctgcgg
    49021 agttacttga tccattactg atgccaatgg acagtccagc ttcggactac ccggtggtga
    49081 atcttccgtt aacgtcttct gtttacttca aaaatggtaa cccagttcgt acatccggtg
    49141 cgccgctgga aggactggtt cgcgttacag aaggtgagaa cggcaaattt atcggtatgg
    49201 gcgaaattga cgatgaaggc cgcgttgcgc ctcgtcgcct ggtggttgaa tacccggcgt
    49261 aatgttaacc gtcttgcgat aacaggtcgc tacgagtaga atactgccgc ttaacgtcgc
    49321 gtaaattgtt taacactttg cgtaacgtac actgggatcg ctgaattaga gatcggcgtc
    49381 ctttcattct atatactttg gagttttaaa atgtctctaa gtactgaagc aacagctaaa
    49441 atcgtttctg agtttggtcg tgacgcaaac gacaccggtt ctactgaagt tcaggtagca
    49501 ctgctgactg cacagatcaa ccacctgcag ggccactttg cagagcacaa aaaagatcac
    49561 cacagccgtc gtggtctgct gcgcatggtt tctcagcgtc gtaaactgct cgactacctg
    49621 aaacgtaaag acgtagcacg ttacacccag ctcatcgagc gcctgggtct gcgtcgctaa
    49681 ttcttgcgag tttcagaaaa gggggcctga gtggcccctt ttttcaagct gacggcagca
    49741 attcactgga aactaatgta ttgttgctat gaatgatctt ccgttgcaga ggttcgcgcg
    49801 gctaatgaga ggctttaccc acatagagct gggttagggt tgtcattagt cgcgaggatg
    49861 cgcagaagat cgggtattaa caccagtgcc gtaaggtact gtctaagaaa gagaaaggat
    49921 attacattgc ttaatccgat cgttcgtaaa ttccagtacg gccaacacac cgtgactctg
    49981 gaaaccggca tgatggctcg tcaggctact gccgctgtta tggttagcat ggatgacacc
    50041 gcggtatttg ttaccgttgt tggccagaaa aaagccaaac caggtcagga cttcttccca
    50101 ctgaccgtta actatcagga gcgtacctac gctgctggtc gtatcccggg tagcttcttc
    50161 cgtcgtgaag gccgcccaag cgaaggcgaa accctgatcg cgcgtctgat tgaccgcccg
    50221 attcggccgc tgttcccgga aggcttcgtc aacgaagttc aggttatcgc caccgtggtt
    50281 tctgttaacc cgcaagttaa cccggatatc gtcgcgatga ttggtgcttc cgcagcactg
    50341 tctctgtctg gtattccgtt caatggtccg attggtgctg ctcgcgtagg ttacatcaat
    50401 gaccagtacg tactgaaccc gactcaggac gagttgaaag agagcaaact ggatctggtt
    50461 gttgccggta ctgaagccgc tgtactgatg gttgaatctg aagctgaact gctgagcgaa
    50521 gaccagatgc tgggcgcagt agtgttcggt catgaacaac agcaggttgt tattcagaac
    50581 atcaatgaac tggtgaaaga agccggtaaa ccacgttggg actggcagcc ggagccggta
    50641 aacgaagcgc tgaacgcgcg cgttgctgca ctggctgaag ctcgcctgag cgatgcttac
    50701 cgcatcaccg acaaacaaga gcgttatgcg caggttgatg tcatcaaatc tgaaaccatc
    50761 gcgacgctgc ttgctgaaga cgaaaccctg gacgaaaacg aactgggtga aattctgcac
    50821 gcgatcgaga aaaacgttgt tcgtagccgc gtactggcag gcgaaccgcg tatcgacggt
    50881 cgtgaaaaag atatgatccg tggtctggat gtgcgtactg gcgtgctgcc gcgtactcac
    50941 ggttctgcgc tgttcacccg tggtgaaacg caggcgctgg ttaccgcaac gctgggtact
    51001 gctcgtgacg cgcaggttct tgatgaactg atgggcgaac gtaccgatac cttcctgttc
    51061 cactacaact tccctccgta ctccgtaggc gaaaccggca tggtcggttc tccgaagcgt
    51121 cgtgaaattg gtcacggtcg tctggcgaag cgcggcgtgc tggcagtcat gccggatatg
    51181 gacaaattcc cgtacaccgt acgtgttgtg tctgaaatca ctgaatccaa cggttcctct
    51241 tctatggctt ccgtgtgcgg cgcgtctctg gcgctgatgg acgcaggtgt gccgatcaaa
    51301 gctgccgttg cgggtatcgc aatgggtctg gtgaaagaag gcgacaacta cgttgtactg
    51361 tctgacattt tgggcgacga agatcacctg ggcgatatgg acttcaaagt tgcgggttcc
    51421 cgcgagggta tctctgcatt gcagatggat atcaaaattg aaggtatcac caaagagatc
    51481 atgcaggttg cgctgaacca ggctaaaggt gcgcgtctgc atatcctggg cgtaatggaa
    51541 caggcgatca acgcgccgcg tggcgatatc tctgagttcg caccacgtat ccataccatc
    51601 aagatcaacc cggacaagat caaagacgtt atcggtaaag gcggctctgt tatccgtgcc
    51661 ctgaccgaag aaactggcac caccatcgaa atcgaagatg acggtactgt gaagattgca
    51721 gcgaccgacg gcgagaaagc gaaacatgct attcgtcgta tcgaagagat cactgcagaa
    51781 atcgaagtgg gccgcgtcta cactggtaaa gtgacccgta tcgttgactt tggcgcattt
    51841 gttgccatcg gcggcggtaa agaaggtctg gtacacatct ctcaaatcgc tgacaaacgc
    51901 gttgagaaag tgaccgatta cctgcagatg ggtcaggaag taccggtgaa agttctggaa
    51961 gttgatcgcc agggccgtat ccgtctgagc attaaagaag cgactgagca gtctcaacct
    52021 gctgcagcac cggaagctcc ggctgctgaa cagggcgagt aaggttgcca tttgccctcc
    52081 gctgcggcgg ggggctttta accgggcagg acgccttgtt agcaaccggg aacaggacgt
    52141 tcattcaacc gtggtcttcg ggagtgggaa atgaagcctt ttttgcgctg gtgtttcgtt
    52201 gcgacagcac ttacgcttgc aggatgcagt aatacttcct ggcgtaaaag tgaagtcctc
    52261 gcggtaccat tgcaaccgac tttacagcag gaagtgattc tggcacgtat ggaacaaatc
    52321 cttgccagtc gggctttaac cgatgacgaa cgcgcacagc ttttatatga gcgcggagtg
    52381 ttgtatgata gtctcggtct gagggcatta gcgcgtaacg atttttcgca agcgctggca
    52441 atccgaccgg atatgcctga agtattcaat tacttaggca tatatttaac gcaggcaggc
    52501 aattttgatg ctgcctatga agcgtttgat tctgtacttg agcttgatcc aacttacaac
    52561 tacgcgcact tgaatcgcgg gattgcatta tattacggcg gtcgtgacaa gttagcgcaa
    52621 gatgatctgc tggcgtttta tcaagacgat cccaatgatc ctttccgtag tctgtggctt
    52681 tatctcgccg agcagaagct cgatgagaag caggctaaag aagtattgaa acagcacttc
    52741 gaaaaatcgg ataaggaaca gtggggatgg aacattgtcg agttctacct gggcaacatt
    52801 agcgaacaaa cgttaatgga aaggctcaag gcggacgcaa cggataacac ctcgctcgct
    52861 gagcatctca gtgaaaccaa cttctattta ggtaagtact acctaagtct gggggatttg
    52921 gacagcgcca cggcactgtt caaactggcg gttgccaaca acgttcataa ctttgttgag
    52981 caccgatacg cattgttgga attatcgctc ctgggccagg accaagatga cctggcagaa
    53041 tcggaccagc aatagctgac gtacacatca gcccgtaatc ttttttgatt gccatcacct
    53101 taacgggtga gggcgttgtt gttcgttaat acacctactt tgagccggtt cacacttttc
    53161 aatgaaaatt gctgatcaat ttcatgatga gttatgtaga ctggccgcca ttaattttga
    53221 ggcacacgta ctacatggct gaattcgaaa ccacttttgc agatctgggc ctgaaggctc
    53281 ctatccttga agcccttaac gatctgggtt acgaaaaacc atctccaatt caggcagagt
    53341 gtattccaca tctgctgaat ggccgcgacg ttctgggtat ggcccagacg gggagcggaa
    53401 aaactgcagc attctcttta cctctgctgc agaatcttga tcctgagctg aaagcaccac
    53461 agattctggt gctggcaccg acccgcgaac tggcggtaca ggttgctgaa gcaatgacgg
    53521 atttctctaa acacatgcgc ggcgtaaatg tggttgccct gtacggcggc cagcgttatg
    53581 acgtgcaatt acgcgccctg cgtcaggggc cgcagatcgt tgtcggtact ccgggccgtc
    53641 tgctggacca cctgaaacgt ggcactctgg acctctctaa actgagcggt ctggttctgg
    53701 atgaagctga cgaaatgctg cgcatgggct tcatcgaaga cgttgaaacc attatggcgc
    53761 agatcccgga aggtcatcag accgctctgt tctctgcaac catgccggaa gcgattcgtc
    53821 gcattacccg ccgctttatg aaagagccgc aggaagtgcg tattcagtcc agcgtgacta
    53881 cccgtcctga catcagccag agctactgga ctgtgtgggg tatgcgtaaa aacgaagcac
    53941 tggtacgttt cctggaagcg gaagattttg atgcggcgat tatcttcgtt cgtaccaaaa
    54001 acgcgactct ggaagtggct gaagctcttg agcgtaacgg ctacaatagc gccgcgctga
    54061 acggtgacat gaaccaggcg ctgcgtgagc agacactgga acgccttaaa gacggtcgtc
    54121 tggacatcct gattgcgacc gacgttgcgg cccgtggcct ggacgttgag cgtatcagcc
    54181 tggtggttaa ctacgatatc ccgatggatt ctgagtctta cgttcaccgt atcggtcgta
    54241 ccggtcgtgc gggtcgtgct ggccgcgcgc tgctgttcgt tgagaaccgc gagcgtcgtc
    54301 tgctgcgcaa cattgaacgt actatgaagc tgactattcc ggaagtagaa ctgccgaacg
    54361 cagaactgct gggcaaacgc cgtctggaaa aattcgccgc taaagtacag cagcagctgg
    54421 aaagcagcga tctggatcaa taccgcgcac tgctgagcaa aattcagccg actgcagaag
    54481 gtgaagagct ggatctcgaa actctggctg cggcactgct gaaaatggca cagggtgaac
    54541 gtactctgat tgttccgcca gatgcgccga tgcgtccgaa acgtgaattc cgtgaccgtg
    54601 atgaccgtgg tccgcgcgat cgtaacgacc gtggcccgcg tggcgaccgt gaagatcgtc
    54661 cgcgtcgtga acgtcgtgat gttggcgata tgcagctgta ccgcattgaa gtgggccgcg
    54721 atgatggtgt tgaagttcgt catatcgttg gcgcgattgc taacgaaggc gacatcagca
    54781 gccgttacat cggtaacatc aagctgtttg cttctcactc caccatcgaa ctgccgaaag
    54841 gtatgccggg tgaagtgctg caacacttta cgcgcactcg cattctcaac aagccgatga
    54901 acatgcagtt actgggcgat gcacagccgc atactggcgg tgagcgtcgt ggcggtggtc
    54961 gtggtttcgg tggcgagcgt cgtgaaggcg gtcgtaactt cagcggtgaa cgccgtgaag
    55021 gtggtcgtgg tgatggtcgt cgttttagcg gcgaacgtcg tgaaggccgc gcaccgcgtc
    55081 gtgatgactc taccggtcgt cgtcgtttcg gtggtgatgc gtaatcatcg ctgaacagcg
    55141 aacacaatct gtaaaataat atatacagcc ccgattttaa ccatcggggc tttttttctg
    55201 tcttttgtac tcatgtactg gtacagtgca atgcataaca acgcagtcgc actatttttc
    55261 actggagaga agccctcatg gcaacactaa ccaccaccca aacgtcaccg tcgctgcttg
    55321 gcggcgtggt gattatcggc ggcaccatta ttggcgcagg gatgttttct ctgccagtgg
    55381 tcatgtccgg ggcgtggttt ttctggtcaa tggcagcgct gatctttacc tggttctgta
    55441 tgctgcattc cggcttgatg attctggaag ctaacctgaa ttaccgaatc ggttcgagtt
    55501 ttgacaccat caccaaagat ttgctgggca aaggctggaa cgtggtcaac ggcatttcca
    55561 ttgcctttgt gctctatatc ctgacctatg cctatatttc tgccagtggt tcgattctgc
    55621 atcacacctt cgcagagatg tcgctaaacg tcccggcacg ggcggcgggt tttggttttg
    55681 cactgctggt agcgtttgtg gtgtggttga gtactaaagc cgtcagccgc atgacggcga
    55741 ttgtgctggg ggcgaaagtc attaccttct tcctcacctt cggtagcctg ctggggcatg
    55801 tgcagcctgc gacattgttc aacgttgccg aaagcaatgc atcttatgcg ccgtatttgc
    55861 tgatgaccct gccgttctgc ctggcgtcgt ttggttatca cggtaacgtg ccaagcctga
    55921 tgaagtatta cggcaaagat ccgaaaacca tcgtgaaatg cctggtgtac ggtacgctga
    55981 tggcactggc gctgtatacc atctggttgc tggcgacaat gggcaacatc cctcgtccgg
    56041 agtttatcgg catcgccgag aagggcggta atattgatgt gttggtacaa gcattaagcg
    56101 gcgtgctgaa cagccgtagc ctggaccttc tgctggtcgt gttctcaaac tttgcggtag
    56161 cgagttcgtt ccttggcgta acgctgggtt tgtttgacta tctggcagat ctgtttggtt
    56221 tcgacgactc ggctgtgggc cgcttgaaaa cggcattgct gacctttgcc ccgcctgttg
    56281 tgggtggtct gctgttcccg aacggattcc tgtatgccat tggttatgct ggtttagcgg
    56341 ctaccatctg ggcggcaatt gttccggcac tgctggcccg cgcatcgcgt aaacgctttg
    56401 gcagcccgaa attccgcgtc tggggtggca agccgatgat tgcgctgatt ctggtgtttg
    56461 gcgtcggcaa cgcactggtg catattttat cgagctttaa tttactgccg gtgtatcagt
    56521 aatcagcggt gccttatccg acatttctgc tgcctacaca atgcctgatg cgcttcgctt
    56581 atcaggccta cgtaggacag cgttgccagc tcggataagg cttcccgcgt taagacacac
    56641 tatcccaaca actcttcctt aacatccatc gccagctcaa acgaatgcag gcgcgcctgg
    56701 tggtcgaaaa tctgcccgtt aaccataatc tcatcagcgt cggtttcgcg caggatcgac
    56761 tgcaaaccat gacgcacttt cgctttatcg cctaccaacg acatactcag cgcctgctgc
    56821 acgtcatact gctcagacgg tgaccagaac tgatccatat tttgaatcgg cggcggcagt
    56881 tgcccggttt cgccacggcg cagcttcaca aaggcttgct gcattgaggt aaacagaaat
    56941 tcagcgtcgc ggttgctgtc ggcggcgata atattgatgc acaccatcgc gtatggtttt
    57001 tccagccgag ccgacggttt gaagttactg cgataaagat gcagcgcctg gaacaacata
    57061 tccggcgcga agtgtgaggc aaacgcaaat ggcagaccaa gctgcgccgc cagttgtgca
    57121 ctgtaaaggc tggagcctaa caaccacacg gggattttct cgccatagcc tggtaccggg
    57181 cgcacatgcg gattgggatc gcgggcgtca aaccagtcca ccagctccgc gacatcgcgg
    57241 gggaaattat caatatcgcc gctcatatga cgacgtagcg ccatcattgt ccgttggtca
    57301 ctacccggag cacgacccag ccccaaatcg attcgccccg gatagagcgt attgagtgtg
    57361 ccgaactgtt cggcaatgac caacggtaag tggttaggca acatcacgcc gccagacccc
    57421 agatgcagcg tggtggtatt cgccgccaga tagccaatca ataccgatgt tgcagcactg
    57481 gcaatgccgg tcatattgtg gtgttctgcc agccagtagc gatgatagcc gcgcttttca
    57541 gccagacggg cgagatcgag agagtgggag aacgcttccc gcgctgaaga gccttcgggt
    57601 atgggggcca gatcgagtag cgaaaacgca atggttttat cagtcataac agctcacttt
    57661 gttaccgatt aatctttaat actgcattaa agaatagtta aagttgttaa caaagtgagc
    57721 tatttactta ggcttgcagc tccagtcctg ccagccgccg ccagtagccg ttacagtcgc
    57781 tgtttgccgt caacggcagt ggcacagcgc cattttcatt agcgcggaag gcgtcgagca
    57841 tcgcgaaagt gtcagtgccc tgcggtgaca ggcggactac atcaaccaat ccctgcatgg
    57901 atgccagctc gttaccgagg ttgtaaacgt agccgctcat ggtctgaatg ccattgagta
    57961 caaacacttg ttggttttcc tgcgacagca cgttgcgccc gttcggatac ttaatgcagc
    58021 aggtttcaca ctcatctttc gggcggtctt ccgaacgcgc ggtaaagcag cgggcggagt
    58081 aggccagcgg tagatgaccg tagctcagga cttccacttc aaactggttg cgaatgccca
    58141 gctcatcgca ctgattaagc agattcacca gccagtcgcg ggaaagctcc accggcatac
    58201 accagcgcat catgccctgt ttgagcaata ttttcagcgt caccgcgttg tagcagttca
    58261 gcgcgtgccc ggcgacgaac ggcagtttgc gttcagcgca catattcacc acgccgagat
    58321 cgctggcttc aatcagaaat tcaccgttct caacatagcg tttcagttcg cccagttcag
    58381 aggatgcctg caccagcgcc agcgtggaga gcacaatctg cttgccactt ccggcgagcg
    58441 attttgccat ctccagacag tcgccaactt tggttgcccg gcgcttgctg cataccgctt
    58501 caccaagata aatcacgtcg gcgctgctgg cggcggcttg ctgataaaaa tcttccagcg
    58561 tctcttttgg ccagtaccac agcactggcc ctaaggaata tttcattgct cttctcactg
    58621 ccatttacgg tgatacgcgc caagggtggt ttgcgtgcct tcggacatcg acccgagcgt
    58681 ctccatccac gcgctttgcg gtacgaagtt ttgcggatcg gccttacaac ggtcgatagc
    58741 ctgacgccag actttcgccg cctggctgac atacgccggg ctacgctggc ggccttcaat
    58801 tttcaccgaa gcaatattcg cagccattaa ctccggcagc agttccaggg tattgagact
    58861 ggttggttct tccagcgcgt gatagcgctc gccgtccacc agataacgcc ctttacacag
    58921 cgtcggataa cctgcgtttt cgccgtcctg ataacggtcg atcagcactt cgttcaggcg
    58981 ggattccagc ccctgcggcg tttgttgcca gcgcacgaaa cgggccggag aacacgcacc
    59041 cacagtgttg ggcgactcac ccgtcagata cgacgacaga tagcaacggc cttccgacat
    59101 aatacacagg ctgccgaaag caaacacttc cagcgggaca ggagtaaccc gtgccagctg
    59161 tttcacctga tgaatcgaca acacgcgcgg cagcaccacg cgggcaacgt caaaatggcg
    59221 atgataaaag ttaatcgcct cttcattggt cgccgaagcc tgcactgaca catgacgttc
    59281 aatatgcggg taacgctcgg cggcgtactc cagcatggcg aggtcggcga ggatcagcgc
    59341 gtcggcaccc agctgcgccg ccatatccac ggcacgctgc caacgggcgt aaccgtccgg
    59401 atgcgcaaaa gtgttaatcg cgatgtgaag tttgcggcga tgttgatgga caaaactcac
    59461 cgcttcctgc aattttttct cggtaaagtt aaggccggcg aagtgacggg cattggtatc
    59521 atcttttagc ccgatataaa cagcatctgc gccgttttcg atggccgcct taagcgccgg
    59581 gagatttccg gcagggcaga gcagctccat aatttttcct gaaggttgcg ctatcaaaac
    59641 gcaaaaatgt taacgaactg ggattttagt taaccttgct gcgacaattt ttgatttaag
    59701 gcagttaaag ttgtattggt ggtagcagca atttcatatg gaattgttga tttataccgc
    59761 tatgttattt ttctgatatg gcaaaatagg atgattgttg aaacagggag taaaactcgt
    59821 gttggataaa ctgcgttccc gtattgtgca tttggggcca tctctgttga gtgtaccggt
    59881 aaaactgacg ccatttgcgc taaaacgcca ggttcttgag caggtcttaa gctggcaatt
    59941 ccgtcaggcg ctggatgatg gcgagctgga gtttcttgaa ggccgctggt taagtattca
    60001 tgtgcgtgat attgacctgc aatggtttac ctcggtggtg aatggcaaac tggtggtcag
    60061 ccagaacacg caagctgatg tgagttttag tgccgacgcc agcgatctgc tgatgattgc
    60121 ggcgcgtaaa caagatccgg atacgctctt cttccagcgt cggctggtga ttgaaggcga
    60181 tacggagctg ggggtgtatg tgaaaaacct gatggacgcc attgaactgg agcaaatgcc
    60241 gaaagcctta cgcatgatgc tgctgcaact ggcggatttt gttgaggcgg gaatgaaaac
    60301 cgcgcctgaa accaaacaga catcggtagg tgaaccatgc taattcgagt agaaattccc
    60361 attgatgcgc cgggtattga tgccctgctg cgtcgctcat tcgaaagtga tgcggaagcg
    60421 aagctggttc acgatctgcg tgaagatggc tttctgacgc tggggctggt ggcgacagat
    60481 gacgaaggtc aggtcattgg ctatgtggca tttagtccgg ttgatgtgca gggcgaagac
    60541 ctgcaatggg tcggcatggc accgttggcg gttgatgaaa aataccgtgg acaggggctg
    60601 gcacgccaac tggtctatga aggactcgat tcgcttaatg agttcggcta tgccgcagtg
    60661 gtgacgctgg gcgatccggc gctgtacagt cgtttcggct ttgaactggc ggcgcatcac
    60721 gatctgcgtt gccgctggcc gggcactgaa agcgccttcc tggtacatcg tttagcggat
    60781 gacgcgctga atggcgtaac cggtctggtt gagtatcacg agcatttcaa tcgcttttaa
    60841 tctccggggt ttgcaggctg ctcaacagtt ctgcaaaccc tgcgccttcc gctaccagac
    60901 gctctttttg ccgtttggtc agttgcttaa cgcgatattc cgcccgtagt gccagcgaac
    60961 ggtcgcccac tggcgcagaa aacgccagcg tcagttctcc tttcccgcgc agtgctttcg
    61021 cccctttgcc gctttggtgc tgctgatagc ggcgttcgac atccgtggtg atcccggtat
    61081 aaagcttatt gtcggcggta cggatcaagt aaagaaacca gggtgtcatc gtctgcatcg
    61141 catgttaagg tcaggcccac agtataaaag agaacagcaa tggaaacact catcgccatc
    61201 agccgttggc tggcaaaaca acatgttgtc acctggtgtg tgcagcagga aggggaactc
    61261 tggtgcgcta atgcctttta tctttttgat gcgcagaaag tcgccttcta cattttgacg
    61321 gaagaaaaaa cgcgccacgc gcagatgagt gggccgcagg ctgcggttgc cggaacggta
    61381 aacggtcagc cgaaaacggt agcgttaatt cgcggtgtgc agtttaaagg tgagatccgc
    61441 aggctggaag gtgaggaaag cgacctcgcg cgcaaggcgt acaatcgtcg ctttccggtt
    61501 gccagaatgc tgtcggcccc ggtgtgggaa atccggctcg atgaaatcaa attcaccgac
    61561 aacacgctgg gctttggtaa aaaaatgatt tggctacgcg actcaggcac cgagcaggcg
    61621 taacgcctcg cggttaaacg ctggcagatc gtccggtgtc cggctggtaa ccagctgatc
    61681 tttatcaacc acgacttcct gatcgtaaaa ttccacgccc gcatttttaa catcaataat
    61741 gatcggttta actgcggtca gtttgcgccc ccgaatcaca tcggcgctga tcagcagctg
    61801 cgga
//
LOCUS       DAJTUP010000011        59537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTUP010000011 DAJTUP010000000
VERSION     DAJTUP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.011"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            328..1617
                     /gene="sdaC"
                     /locus_tag="OO468_000711"
     CDS             328..1617
                     /gene="sdaC"
                     /locus_tag="OO468_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR6257938.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1675..3042
                     /gene="sdaB"
                     /locus_tag="OO468_000712"
     CDS             1675..3042
                     /gene="sdaB"
                     /locus_tag="OO468_000712"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR6257939.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3153..3908
                     /gene="xni"
                     /locus_tag="OO468_000713"
     CDS             3153..3908
                     /gene="xni"
                     /locus_tag="OO468_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR6257940.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(3961..5109)
                     /gene="fucO"
                     /locus_tag="OO468_000714"
     CDS             complement(3961..5109)
                     /gene="fucO"
                     /locus_tag="OO468_000714"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR6257941.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5137..5784)
                     /gene="fucA"
                     /locus_tag="OO468_000715"
     CDS             complement(5137..5784)
                     /gene="fucA"
                     /locus_tag="OO468_000715"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR6257942.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6331..7647
                     /gene="fucP"
                     /locus_tag="OO468_000716"
     CDS             6331..7647
                     /gene="fucP"
                     /locus_tag="OO468_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR6257943.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7680..9455
                     /gene="fucI"
                     /locus_tag="OO468_000717"
     CDS             7680..9455
                     /gene="fucI"
                     /locus_tag="OO468_000717"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR6257944.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9564..10983
                     /gene="fucK"
                     /locus_tag="OO468_000718"
                     /pseudo
     CDS             9564..10983
                     /gene="fucK"
                     /locus_tag="OO468_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            10985..11407
                     /gene="fucU"
                     /locus_tag="OO468_000719"
     CDS             10985..11407
                     /gene="fucU"
                     /locus_tag="OO468_000719"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR6257945.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11465..12196
                     /gene="fucR"
                     /locus_tag="OO468_000720"
     CDS             11465..12196
                     /gene="fucR"
                     /locus_tag="OO468_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR6257946.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12240..13340)
                     /gene="rlmM"
                     /locus_tag="OO468_000721"
     CDS             complement(12240..13340)
                     /gene="rlmM"
                     /locus_tag="OO468_000721"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR6257947.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13333..13728)
                     /locus_tag="OO468_000722"
     CDS             complement(13333..13728)
                     /locus_tag="OO468_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR6257948.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13747..14664)
                     /gene="gcvA"
                     /locus_tag="OO468_000723"
     CDS             complement(13747..14664)
                     /gene="gcvA"
                     /locus_tag="OO468_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR6257949.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15015..15242)
                     /locus_tag="OO468_000724"
     CDS             complement(15015..15242)
                     /locus_tag="OO468_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR6257950.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15434..16639
                     /gene="csdA"
                     /locus_tag="OO468_000725"
     CDS             15434..16639
                     /gene="csdA"
                     /locus_tag="OO468_000725"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR6257951.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16639..17082
                     /gene="csdE"
                     /locus_tag="OO468_000726"
     CDS             16639..17082
                     /gene="csdE"
                     /locus_tag="OO468_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR6257952.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17133..17939)
                     /gene="tcdA"
                     /locus_tag="OO468_000727"
     CDS             complement(17133..17939)
                     /gene="tcdA"
                     /locus_tag="OO468_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR6257953.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18177..19274)
                     /gene="mltA"
                     /locus_tag="OO468_000728"
     CDS             complement(18177..19274)
                     /gene="mltA"
                     /locus_tag="OO468_000728"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR6257954.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19483..19559
                     /locus_tag="OO468_000729"
     tRNA            19483..19559
                     /locus_tag="OO468_000729"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19517..19519,aa:Met,seq:cat)
     gene            complement(19634..20887)
                     /gene="amiC"
                     /locus_tag="OO468_000730"
     CDS             complement(19634..20887)
                     /gene="amiC"
                     /locus_tag="OO468_000730"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR6257955.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21119..22450
                     /gene="argA"
                     /locus_tag="OO468_000731"
     CDS             21119..22450
                     /gene="argA"
                     /locus_tag="OO468_000731"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR6257956.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22512..24338)
                     /gene="recD"
                     /locus_tag="OO468_000732"
     CDS             complement(22512..24338)
                     /gene="recD"
                     /locus_tag="OO468_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR6257957.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24338..27880)
                     /gene="recB"
                     /locus_tag="OO468_000733"
     CDS             complement(24338..27880)
                     /gene="recB"
                     /locus_tag="OO468_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR6257958.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(27873..30761)
                     /gene="ptrA"
                     /locus_tag="OO468_000734"
     CDS             complement(27873..30761)
                     /gene="ptrA"
                     /locus_tag="OO468_000734"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR6257959.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(30937..34305)
                     /gene="recC"
                     /locus_tag="OO468_000735"
     CDS             complement(30937..34305)
                     /gene="recC"
                     /locus_tag="OO468_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR6257960.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34318..34668)
                     /locus_tag="OO468_000736"
     CDS             complement(34318..34668)
                     /locus_tag="OO468_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR6257961.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34653..35060)
                     /locus_tag="OO468_000737"
     CDS             complement(34653..35060)
                     /locus_tag="OO468_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR6257962.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35057..35620)
                     /locus_tag="OO468_000738"
     CDS             complement(35057..35620)
                     /locus_tag="OO468_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR6257963.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35611..36081)
                     /locus_tag="OO468_000739"
                     /pseudo
     CDS             complement(35611..36081)
                     /locus_tag="OO468_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36265..37059)
                     /gene="thyA"
                     /locus_tag="OO468_000740"
     CDS             complement(36265..37059)
                     /gene="thyA"
                     /locus_tag="OO468_000740"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR6257964.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37066..37941)
                     /gene="lgt"
                     /locus_tag="OO468_000741"
     CDS             complement(37066..37941)
                     /gene="lgt"
                     /locus_tag="OO468_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR6257965.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38092..40338)
                     /gene="ptsP"
                     /locus_tag="OO468_000742"
     CDS             complement(38092..40338)
                     /gene="ptsP"
                     /locus_tag="OO468_000742"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6257966.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40351..40881)
                     /gene="rppH"
                     /locus_tag="OO468_000743"
     CDS             complement(40351..40881)
                     /gene="rppH"
                     /locus_tag="OO468_000743"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR6257967.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41566..42255
                     /gene="mutH"
                     /locus_tag="OO468_000744"
     CDS             41566..42255
                     /gene="mutH"
                     /locus_tag="OO468_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR6257968.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42324..43037
                     /locus_tag="OO468_000745"
     CDS             42324..43037
                     /locus_tag="OO468_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6257969.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43175..43393
                     /gene="ygdR"
                     /locus_tag="OO468_000746"
     CDS             43175..43393
                     /gene="ygdR"
                     /locus_tag="OO468_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR6257970.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43501..44541
                     /locus_tag="OO468_000747"
     CDS             43501..44541
                     /locus_tag="OO468_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR6257971.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44574..45764)
                     /gene="lplT"
                     /locus_tag="OO468_000748"
     CDS             complement(44574..45764)
                     /gene="lplT"
                     /locus_tag="OO468_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR6257972.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(45757..47916)
                     /gene="aas"
                     /locus_tag="OO468_000749"
     CDS             complement(45757..47916)
                     /gene="aas"
                     /locus_tag="OO468_000749"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR6257973.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48502..49533
                     /gene="galR"
                     /locus_tag="OO468_000750"
     CDS             48502..49533
                     /gene="galR"
                     /locus_tag="OO468_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR6257974.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49540..50802)
                     /gene="lysA"
                     /locus_tag="OO468_000751"
     CDS             complement(49540..50802)
                     /gene="lysA"
                     /locus_tag="OO468_000751"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR6257975.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            50924..51859
                     /gene="lysR"
                     /locus_tag="OO468_000752"
     CDS             50924..51859
                     /gene="lysR"
                     /locus_tag="OO468_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR6257976.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(51846..52538)
                     /gene="ygeA"
                     /locus_tag="OO468_000753"
     CDS             complement(51846..52538)
                     /gene="ygeA"
                     /locus_tag="OO468_000753"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR6257977.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52667..54085)
                     /gene="araE"
                     /locus_tag="OO468_000754"
     CDS             complement(52667..54085)
                     /gene="araE"
                     /locus_tag="OO468_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR6257978.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54400..55161)
                     /gene="kduD"
                     /locus_tag="OO468_000755"
     CDS             complement(54400..55161)
                     /gene="kduD"
                     /locus_tag="OO468_000755"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR6257979.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55191..56027)
                     /gene="kduI"
                     /locus_tag="OO468_000756"
     CDS             complement(55191..56027)
                     /gene="kduI"
                     /locus_tag="OO468_000756"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR6257980.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56314..57495)
                     /locus_tag="OO468_000757"
     CDS             complement(56314..57495)
                     /locus_tag="OO468_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR6257981.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            57750..58979
                     /locus_tag="OO468_000758"
                     /pseudo
     CDS             57750..58979
                     /locus_tag="OO468_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14688 a        15987 c        14518 g        14344 t
ORIGIN      
        1 tgattttttc agttcaacct acatatattg cgcgccccgg aagaagtcag atgtcgttta
       61 atgggcaaat attgccctta aattctcttt tacttttgat ttacagagta aagcgttggg
      121 ataatctatc ttccaagcag attattgtat ttgggatcaa gatcactgat agatacataa
      181 cttgtgtgta tctttccgcc ctcaaattat tacggcggta aatgattaag ccatcgccga
      241 tagacagatt tcatttttac ggtcaggcac cttcccgggc tgaactggct aaaagctgaa
      301 ttatttgcat tcctccagga gaaatagatg gaaacgactc aaaccagcac gattgcgtcg
      361 aaagactctc gtagtgcctg gcgcaagaca gacaccatgt ggatgctggg cctttacggc
      421 acggcaatcg gcgcgggcgt gctgttcctg ccaatcaacg ccggtgttgg cggtatgatc
      481 ccgctgatca tcatggctat ccttgcgttc ccgatgacgt tttttgctca ccgcggcctg
      541 actcgcttcg tactgtctgg taaaaacccg ggcgaagaca tcaccgaggt tgtagaagaa
      601 cactttggta ttggcgcagg taaactgatt accctgctct acttcttcgc tatctacccg
      661 atcctgctgg tttatagcgt ggcaatcacc aataccgttg aaagcttcat gtctcaccag
      721 ctgggtatga cgccaccgcc gcgtgcgatt ctgtcgctga tcctgatcgt gggtatgatg
      781 accatcgttc gctttggtga gcagatgatc gttaaagcga tgagtattct ggtattcccg
      841 tttgttggcg tactgatgct gctggctctg tacctgatcc cgcagtggaa cggcgcagca
      901 ctggaaacgc tgtctctgga cactgcatct gcaaccggaa acggtctgtg gatgaccctg
      961 tggctggcaa ttccggtaat ggtgttctcg ttcaaccact ctccgatcat ctcttctttc
     1021 gccgttgcga agcgtgaaga gtacggcgat atggcagaac agaaatgctc gaagatcctg
     1081 gcattcgcac acatcatgat ggtgctgacc gtaatgttct tcgtcttcag ctgcgtactg
     1141 agcctgactc cggcagacct ggctgcggct aaagagcaga acatctcgat tctgtcttac
     1201 ctggctaacc actttaacgc accggttatc gcgtggatgg ctccgattat cgcgattatc
     1261 gctatcacca aatccttcct cggccactac ctgggcgcac gtgaaggctt caacggtatg
     1321 gtgattaaat ctctgcgtgg taaaggtaag tctatcgaaa tcaacaagct gaaccgtatc
     1381 actgcgctgt tcatgctggt aacgacctgg attgttgcca ccctgaaccc gagcatcctg
     1441 ggtatgattg aaaccctggg cggcccaatc atcgcgatga tcctgttcct gatgccgatg
     1501 tacgcaattc agaaagtacc ggcaatgcgt aagtacagcg gtcacatcag caacgtattc
     1561 gttgtcgtga tgggtctgat tgcaatctcc gcaatcttct actctctgtt cagctaagtc
     1621 ctttcgcgcc gctttcgggc ggcgcttcct ccgttttaac gcgatgtatt tcctatgatt
     1681 agcgtattcg atattttcaa aatcggcatt ggcccttcca gttctcatac cgttggacca
     1741 atgaaagcgg gtaaacaatt taccgacgat ctgattgccc gtaacctgct taaagacgtg
     1801 acccgcgtgg tggttgacgt gtacggctcg ctctctctga ctggtaaagg ccaccacaca
     1861 gatatcgcca ttattatggg cctggcgggt aacctgccgg ataccgtgga tatcgattcc
     1921 atccccggtt ttattcagga tgtgaatact catggtcgcc tgatgctggc aaacggtcag
     1981 catgaagtgg agttcccggt tgatcagtgc atgaacttcc atgcagacaa cctttctctg
     2041 catgaaaacg gtatgcgcat taccgcgctg gcgggcgata aagtcgttta cagccagact
     2101 tactactcta ttggcggtgg ctttatcgtt gatgaagagc attttggcca gcaggatagc
     2161 gcaccggttg aagttcctta tccgtacagt tcagcagccg atctgcaaaa acattgtcag
     2221 gaaaccgggc tgtcactctc tggcctgatg atgaaaaacg agctggcgct gcacagtaaa
     2281 gaagagctgg aacagcacct ggcgaacgtc tgggaagtca tgcgtggcgg tattgagcgc
     2341 ggtatttcca ccgaaggcgt gttgcctggc aaactgcgcg ttccacgccg tgctgcggca
     2401 ctacgccgga tgctggtcag ccaggataaa accaccactg acccgatggc ggttgttgac
     2461 tggatcaaca tgtttgcact ggcagtgaac gaagagaacg ctgctggcgg tcgcgtggtg
     2521 actgcgccga ctaacggtgc gtgcgggatt atcccggctg tgctggcgta ctacgataag
     2581 tttatccgcg aagtgaacgc taactcactg gctcgttacc tgctggtagc cagcgccatt
     2641 ggttctcttt ataagatgaa cgcgtcgatt tctggtgccg aagtgggttg ccagggtgaa
     2701 gttggcgtgg cgtgctctat ggcggcagct ggtctggcag aactgttagg cgcaagcccg
     2761 gcgcaggttt gcatcgcagc ggaaatcgcg atggagcaca acctcggtct gacttgtgac
     2821 ccggtcgcgg ggcaggttca ggtaccgtgc attgagcgta acgccattgc agcagtgaaa
     2881 gcggtgaacg ccgcgcgtat ggcactgcgc cgtaccagcg aaccgcgcgt ctgcctcgat
     2941 aaagttatcg aaaccatgta cgaaacaggt aaagacatga acgccaagta ccgcgaaacc
     3001 tctcgcggcg gcctggcgat gaagatcgtt gcctgcgatt aatctctccc agaggcctcg
     3061 ttttgcgaag cctcttcctg ttttctcaac cagccatagc ctgttccggc atcgaatgtt
     3121 acccttatcg cctgatcttt aagggggtta tcgtggctgt tcatttgctt attgtcgatg
     3181 cactgaatct tatccgtcgc attcatgccg ttcagggttc gccctgtgtc gaaacctgcc
     3241 agcatgcgct cgatcagctc attatgcaca gccagccaac ccacgcggtc gccgtttttg
     3301 atgatgaaaa ccgcagtagc ggctggcgtc atcagcgttt accagattac aaagcggatc
     3361 gaccgccaat gccggaagag ttgcacgacg agatgcctgc attacgcgcc gcctttgagc
     3421 aacgcggcgt cccgtgctgg tcagccagcg gcaacgaagc cgatgactta gccgccacac
     3481 tggcggtcaa agtgacacag gccgggcatc aggcaacgat tgtttcgaca gataaaggct
     3541 actgtcagtt actttcaccg acattacgta ttcgtgatta cttccagaaa cgttggctgg
     3601 atgcgccatt tatcgataaa gaatttggcg ttcaaccgca gcagttgccc gattactggg
     3661 gacttgcggg gatcagcagt tcaaaggtac cgggtgttgc gggaatcgga ccaaaaagcg
     3721 ccacgcagct gctggtcgag tttcagagtc tggaagggat atatgagaat ctggatgcgg
     3781 ttgccgaaaa gtggcgcaaa aaattagaaa cccataaaga gatggcgttt ctgtgccgcg
     3841 atattgcccg cttacaaacc gatttgcata tcgacggcaa tttacagcaa ttgcggttgg
     3901 tacggtaacg gcgagccgga tacgccgcaa atcgtatccg gcattatcac atcagcgcat
     3961 ttaccaggcg gtatggtaaa gctctacaat atcctcaagc gttgcttcac gcgggttgcc
     4021 accggtacaa acatcatcca gtgccgcctg agccagtgcc gaaatgtctt ccttgcgtac
     4081 accaacatca cgcaaatgtg gcggaatacc gacatcacgg ttgagggcaa acaccgcttc
     4141 aacagcggca ttacgcgccc cttccaggct cataccttcc actttcacgc ccataacgcg
     4201 cgcgatatcg cggtacttct caccggtaaa gtcagcgtta tagcgcatga catgcggtaa
     4261 caggatggcg ttcgcaacac cgtgtggagt gttataaaac gcgcccagtg gatgcgccat
     4321 accatgcacc aaccctaacc caacattcga gaagcccata cccgcaacat actgcccgag
     4381 cgccatttct tctccggcat ccttatcacc agcaaccgat cctcgcagcg ccccagcaat
     4441 gatttcaatc gctttaatgt gcagtgcatc ggttagtgcc cacgcgccac gggtaatata
     4501 cccctcaata gcatgagtga gcgcatcaac acccgtcgcg gctttcagcg ccggaggcat
     4561 accatccatc atgtcagcat caataaacgc cacctgcggg atatcatgcg gatcaacgca
     4621 aacaaacttg cgccgttttt cttcgtcagt gatcacgtag ttaatggtca cttctgccgc
     4681 agtacctgct gtggtgggga ttgccagaat cggtacactg ggtttattgg tcggggaaag
     4741 cccttccagg ctacgcacat cggcaaactc cgggttgttg ctgataatgc caattgcttt
     4801 acaagtatcc tgtggagaac caccaccaat agcgatcagg taatccgcgc cgctattctg
     4861 gaatacaccg agcccttctt tgacgacagt aattgttggg ttgggcacta cgccgtcgta
     4921 aatcgcccat gccagccctg cagcatccat cttatcggtc actttcgcca ccacgccgca
     4981 ttgcaccagc gttttatcgg tgacgatcag cgccttctga taaccacggc gtttcacctc
     5041 atcggttaaa gccccaacag caccccgacc aaaccatgcc gtttcgttca gaatcattct
     5101 gttagccatc atccttctcc ttgttgcttt acgaaattac tcttcaatcc gtaacccata
     5161 ggttttgaat ttctccagca ctacggcaat ctcttcatcg ctcagcactg gcaccgggtc
     5221 cgtaatcgcc agggtcgtca ggtaaagttg cgccagcact tcaacttcat gcgccagcca
     5281 taacgctttt tccagattca cctcacaagc gataagccca tgatgttgta acaaagttgc
     5341 cttacgattt ttgagagcca gcgcaacatg ttcagaaagt tcgcgtgttc caaaggtcgc
     5401 ataaggcgcg caaggaatag aattaccgcc agccgccgca atcatgtagt gaatagcggg
     5461 gatcgatcgg ttaagaatgg aaactgccgt gcaatgaacg gcatgattgt gaacaaccgc
     5521 gttggcatcc ggtctgcttt gataggctgc catatggaaa cgccattcgc ttgaggggag
     5581 ctttccttcc tcatgtttac cgttgccatc aataaagaca atatgcgact ccgtcagttt
     5641 ttcatatgga atgcctgtag gcgtaatcag catcccatcc tgataacgta cactgacgtt
     5701 ccccgctgtc ccctggttca gtcccaggcg ggtcatttcc aggcaagtgt caataatctg
     5761 acgagcaagt ttatttcgtt ccattagcta cctctctctg attcaaaaca gggcaataat
     5821 gttgttcctt tcacactatt gaattagccg tttaattacc caccatcttc ttcctgatta
     5881 acaagaaaga aattcacaag cttatatttt gtgacctagc tcaactaatc acagtaaata
     5941 actgcaagtt ctctttttat aaccccgtta aaaatgaccg ctcttaaaaa tatttatcaa
     6001 aacggtcatt tttccatttc ccctcgccag aaatgcccgt tttcggcaca aacaattaat
     6061 acggtcatct ggtttgtgtt ttttatgatt tattttctga aacgggcatg aaatttcgat
     6121 tattaaagtg atggtagtca cataaagtca ccttccagct aataagtgtg accgccgtca
     6181 tattacagag cgttttttat ttgaaaatga atccatgagt tcatttcaga caggcaaata
     6241 ttcaccgata tgaagcccga actcgctggt tttgcacttt tgaaaacata accgattacg
     6301 tgcttaagct tctgaaccta agaggatgct atgggaaaca catcaataca aacgcagagt
     6361 taccgtgcgg tagataaaga tgcagggcaa agcagaagtt acattattcc attcgcgctg
     6421 ctgtgctcac tgttttttct ttgggcggta gccaataacc ttaacgacat tttattacct
     6481 caattccagc aggcttttac gctgacaaat ttccaggctg gcctgatcca atcggccttt
     6541 tactttggtt atttcattat cccaatccct gctgggatat tgatgaaaaa actcagttat
     6601 aaagcaggga ttattaccgg attattttta tatgccttgg gcgctgcatt attctggccc
     6661 gctgcagaaa taatgaacta caccttattt ttagttggcc tatttattat tgcagccgga
     6721 ttaggttgtc tggaaactgc cgcaaaccct tttgttacgg tattagggcc ggaaagcagt
     6781 agtcacttcc gcttaaatct tgcgcaaaca tttaactcgt ttggcgcaat tatcgcggtt
     6841 gtctttgggc aaagtcttat tttgtctaac gtgccacatc aatcgcaaga cgttctcgat
     6901 aaaatgtctc cagagcaatt gagtgcgtat aaacacagcc tggtattatc ggtacagaca
     6961 ccttatatga tcatcgtggc tatcgtgtta ctggtcgccc tgctgatcat gctgacgaaa
     7021 ttcccggcat tgcagagtga taatcacagt gacgccaaac aaggatcgtt ctccgcatcg
     7081 ctttctcgtc tggcgcgtat tcgtcactgg cgctgggcgg tattagcgca attctgctat
     7141 gtcggcgcac aaacggcctg ctggagctat ttgattcgct acgctgtaga agaaattcta
     7201 ggtatgactg caggctttgc cgctaactat ttaaccggaa ccatggtgtg cttctttatt
     7261 ggtcgtttca ccggtacctg gctcatcagt cgcttcgcac cacacaaagt cctggccgcc
     7321 tacgcattaa tcgctatggc actgtgcctg atctcagcct tcgctggcgg tcatgtgggc
     7381 ttaatagccc tgactttatg cagcgccttt atgtcgattc agtacccaac aatcttctcg
     7441 ctgggcatta agaatctcgg ccaggacacc aaatacggtt cgtccttcat cgttatgacc
     7501 attattggcg gcggtattgt cactccggtc atgggttttg tcagtgacgc ggcgggcaac
     7561 atccccactg ctgaactgat ccccgcactc tgcttcgcgg tcatctttat ctttgcccgt
     7621 ttccgttctc aaacggcaac taactgaaca tattttccga ataaagtgag gaatctgtaa
     7681 tgaaaaaaat cagcttaccg aaaattggta tccgcccggt tattgacggt cgtcgcatgg
     7741 gtgttcgtga gtcgcttgaa gaacaaacaa tgaatatggc gaaagctacg gccgcactgc
     7801 tgaccgagaa actgcgccat gcctgcggag ctgccgtcga gtgtgtcatt tccgatacct
     7861 gtatcgcggg tatggctgaa gccgctgctt gcgaagaaaa attcagcagc cagaatgtag
     7921 gcctcaccat tacggtaacg ccttgctggt gctatggcag tgaaaccatc gacatggatc
     7981 caacccgccc gaaagccatt tggggcttta acggcactga acgccccggc gctgtttacc
     8041 tggctgcggc tctggcagct cacagccaga aaggcatccc ggcattctcc atttacggtc
     8101 atgacgttca ggatgccgat gacacatcga ttcctgccga tgttgaagaa aaactgctgc
     8161 gctttgcccg cgccggtttg gccgtcgcca gcatgaaagg taaaagctat ctgtcgctgg
     8221 gcggcgtttc gatgggtatc gccggttcta ttgttgatca caacttcttt gaatcctggc
     8281 tgggaatgaa agtccaggcg gtggatatga ccgaactgcg tcgccgtatc gatcagaaga
     8341 tttacgacga agccgaactg gaaatggcac tggcctgggc tgataaaaac ttccgctatg
     8401 gcgaagatga aaataacaaa cagtatcaac gcaatgccga gcaaagccgc gcagttctgc
     8461 gcgaaagttt actgatggcg atgtgtatcc gcgacatgat gcaaggcaac agcaaactgg
     8521 ccgatattgg tcgcgtggaa gaatcacttg gctacaacgc catcgctgcg ggcttccagg
     8581 ggcaacgtca ctggaccgat caatatccca atggtgacac cgccgaagcg atcctcaaca
     8641 gttcatttga ctggaatggc gtgcgcgaac cctttgtcgt ggcgaccgaa aacgacagtc
     8701 ttaacggcgt ggcaatgcta atgggtcacc agctcaccgg caccgctcag gtatttgccg
     8761 atgtgcgtac ctactggtca ccagaagcaa ttgagcgtgt aacggggcat aaactggacg
     8821 gactggcaga acacggcatc atccatttga tcaactcagg ttctgctgcg ctggacggtt
     8881 cctgtaaaca acgcgacagc gaaggtaacc cgacgatgaa gccgcactgg gaaatctctc
     8941 agcaagaggc tgacgcttgc ctcgccgcta ccgaatggtg cccggcgatc cacgaatact
     9001 tccgtggcgg cggttactct tcccgcttcc ttaccgaagg cggcgtcccg ttcaccatga
     9061 ctcgtgtcaa catcatcaaa ggcctgggac cggtactgca aatcgcggaa ggctggagcg
     9121 tggaattgcc gaaggatgtg catgacatcc tcaacaaacg caccaactca acctggccaa
     9181 ccacctggtt tgcaccgcgc ctcactggta aagggccgtt tacggatgtg tattcggtaa
     9241 tggcgaactg gggtgccaac cacggagttc tgaccatcgg ccacgttggc gcagacttta
     9301 tcacccttgc ctccatgctg cgtatcccgg tatgtatgca caacgttgaa gagaccaaag
     9361 tgtatcgtcc ttctgcctgg gctgcgcacg gcatggatat tgaaggccag gattaccgcg
     9421 cttgccagaa ctacggtccg ttgtacaagc gttaatacat tacaccctga tgtgatgccc
     9481 ggtcgctccg gctaccgggc ctgaacaagc aagagtggtt agccggataa acaatgttat
     9541 ccggctatat tgcaggagcg attatgaaac aagaagttat cctggtactc gactgtggcg
     9601 cgaccaatgt cagggccatc gcggttaatc ggcagggaaa aattgttgcc cgcgcctcaa
     9661 cgcctaatgc cagcgatatc gcgatggaaa acaacacctg gcaccagtgg tctttagact
     9721 gccattttgc aacgctttgc tgattgctgt cggcaaatca atagtgaact gactgaatgc
     9781 cacatccgcg gtatcgccgt caccaccttt agtgtggatg gcgctctggt agataagcaa
     9841 ggcaatctgc tctatccgat cattagctgg aaatgtccgc gaacagcagc ggttatggac
     9901 aatattgaac ggttaatctc cgcacagcgg ttgcaggcta tttctggcgt cggagccttt
     9961 agtttcaata cgttatataa gttggtgtgg ttgaaagaaa atcatccaca actgctggaa
    10021 cgcgcgcacg cctggctctt catttcgtcg ctgattaacc accgtttaac cagcgaattc
    10081 actactgata tcacgatggc cggaaccagc cagatgctgg atatccagca acgcgatttc
    10141 agtccgcaaa ttttacaagc caccggtatt ccacgccgac tcttccctcg tctggtggaa
    10201 gcgggtgaac agattggtac gctacagaac aacgccgcag caatgctcgg cttacccgtt
    10261 ggcataccgg tgatttccgc aggtcacgat acccagttcg ccctttttgg cgctggtgcc
    10321 gaacaaaatg aacccgtgct ctcctccggt acatgggaaa ttttaatggt tcgcagcgcc
    10381 taggttgata cttcgctgtt aagtcagtac gccggttcca cctgcgaact agatagccag
    10441 gcatggttgt ataacccagg tatgcaatgg ctggcatccg gcgtgctgga atgggtgaga
    10501 aaactgttct ggacggctga aacaccctgg caaatgttga ttgaagaagc tcgtctgatc
    10561 gcacctggcg cggatggtgt gaaaatgcag tgtgatttat tgtcgtgtca gaacgctggc
    10621 tggcaaggag tgacgcttaa taccacgcgg gggcatttct atcgcgcggc gctggaaggg
    10681 ttaactgcgc aattacagcg caatctacag atgctggaaa aaatcgggca ctttaaagcc
    10741 tatgaattat tgttagtcgg tggaggaagt cgcaacacat tgtggaatca gattaaagcc
    10801 aatatgcttg atattccggt aaaagttctc gacgatgccg aaacgaccgt cgcaggagct
    10861 gcgctgttcg gttggtatgg cgtaggggaa tttaacagcc cggaagaagc ccgtgcacag
    10921 attcattatc agtaccgtta tttctacccg caaactgaac ctgaatttat agaggaagtg
    10981 tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa ctattgaaag tgctggcaga
    11041 gatgggacat ggagatgaaa ttattttttc cgatgctcac tttcccgccc attcgatgga
    11101 accgcaggtg atccgcgctg atggcctgtt ggtgagcgac ttgctccagg cgattatccc
    11161 gttatttgag ctggacagtt atgccccgcc gctggtgatg atggcggcgg tagagggtga
    11221 cactctcgat cctgaagtag aacgacgtta ccgtaatgcg ctttcactac aagccccgtg
    11281 tcctgacatc atccgcatca atcgttttgc gttttatgaa cgggcgcaaa aagcctttgc
    11341 gatcgttatc acaggcgaac gagcgaagta cgggaatatt cttttaaaaa aaggggtaac
    11401 accgtaatct cataccggta cgcccgcatg acgcgggcgg ttatcgaatg atggggtgaa
    11461 aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc ataccagcct
    11521 gaccacggaa gctctctctg aacagctaaa ggtcagtaaa gaaaccattc gtcgcgatct
    11581 caatgaatta cagacgcagg gtaaaattct gcgcaatcat ggacgcgcta aatatatcca
    11641 ccgtcaaaat caagacagtg gcgatccctt tcacatcagg ctgaaaagcc attatgcgca
    11701 taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg tgatagcctt
    11761 agacgccagt tcaacttgct ggtatctggc acgccagttg cctgacatca acattcaggt
    11821 cttcaccaat agccatccga tttgccatga actcggtaaa cgcgaacgca ttcaactgat
    11881 cagttccggc ggcacacttg agcgcaaata tggctgttac gtcaatccct cgctgatttc
    11941 ccaacttaaa tcgctggaaa tcgatctgtt tattttttct tgtgaaggga tcgatagcag
    12001 cggcgcactg tgggactcca atgcgatcaa cgctgattac aaatcgatgc tattaaaacg
    12061 tgccgcgcaa tcgttgttat tgattgataa aagtaaattt aatcgttcag gggaagcccg
    12121 catcgggcat ctggatgagg taacgcacat tatttctgat gagcgccagg ttgcaacttc
    12181 tttggtaaca gcctgacgga aagggtagca ggccggagac gacgccccgg ccttgcctgt
    12241 tatcgctcgt cgcgacgacc acccaccgca gcccagatgc ggcggacgtg caccgtcact
    12301 tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc atgttcatca
    12361 agctgtgcct gaatatacgc cagattgtgt gatacttctt cgtagcgttt tttcatcggc
    12421 agtttgagat tgaaaatggt ttcacggcac cagccattaa ccagccactg cgccatcaat
    12481 gccgcaactt tagccggttt ttcaaccata tcgcatacca tccaggagat attgctgcgt
    12541 gtcggacgga atttgaaacc gtcttcccgc agccacgtca cctgcccggt atccatcaga
    12601 ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt caccagttgg
    12661 taggtccagc cgccagggca agcgcctaaa tccaccgccc acatcccgtt cgccaggcgt
    12721 tcatcccact catccgcagg aataaacaca tgaaatgcct cttccagttt gagcgtggaa
    12781 cgactcggcg cctctgccgg aaatttcagg cgcggaatgc ccatatagaa cggcgaatta
    12841 ttgttgctgt atgagtaacc ggtatagcag catcctggtg caataaagaa tacatgcaca
    12901 accggacgct tcggcgtttc atagttcgcc agcaccccag catcgcgcaa ggcagcgcgt
    12961 agcggaacag tgaacttacg gcagaatttc agcaactctt tgctttcgtt ggtatcggca
    13021 acttcaacac gcagttcacc gcccttctct actacaccct gtaacatgcc gacaatgggg
    13081 gtaatacgat cttctggcgg caaatgctgc aggagttccc ccaccacaaa ccactggcgg
    13141 gcaaaaatta atgaactgaa cggcagctca cggattaact tatcgccatc atcaggttga
    13201 taacattcat aaatgacata acccgcattc tctttcacgc gggcaaaacc gaaaatttcc
    13261 cgctggccgg ctttatcggt aatttctgcg gcgcactctt tttcaaagcc cggacggcac
    13321 agcaatacaa ccttattcat gacttacgcc cttgcgcttt aaacggatag caccaactaa
    13381 cattaacgcc cagcccgcaa ggaagctcac gccgccaacc ggtgtgacaa acgcccacaa
    13441 acgcagatgg gacagcgcca ggcaataaag gctgccgctg aacagcaccg tgcctaacgc
    13501 gaggaaaacg ctactccagt aaaaccagat actgatgcga cgctgcattg ccaccgccag
    13561 acctaagatc gccagcgtat gaaacgcctg gtattcgaga ccggtttgga tccagcccat
    13621 ctcaacggcc cccatggttt tacttaacac atgcgcacca aaagcgccca gtgccacaaa
    13681 aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg tcgtacccta
    13741 cgtaaattat tgttcataac gaaagcggaa tttttcttgt tcagcagcgg ctttcgccag
    13801 gatccattgg cgaaaggcgg ctattttacc cagttctgcc tggctgtcat gacaaaccag
    13861 ataaaaagca tttttactga ccagaacatc attaaacggg caaacaagac gtccggcctc
    13921 gatttcagat tgcgccatca cgttatttgc cagcgccact ccctgcccgt gaatagccgc
    13981 ttgcagcacc atggcgctat gactaaaaat tggcccttgc tgaacgttga tatgattaaa
    14041 ccccaactgt cgggtatatg tctgccagtc acggcgtgac gcatcatgta ataacgtatg
    14101 tttagccaga tcttccgggg tcttcaaggg tttttcgcct gtcagcagta gcggcgaaca
    14161 caccggcaat aaatattcgg cgtacagttt ttccacccgt agccccggcc agttgccccg
    14221 accataaaat atcgccacat caacatcatc cgccagctta tcttcctgac gatcaaccgc
    14281 ctggattcga acgtcaattc ccggataagc tgaattaaag ctggaaagtc gcggaaccaa
    14341 ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac gcccccttgg cgctacgggc
    14401 ctggagttta cgcgtcgctt cggttaattg cgaaaatatc tctttgatat cgaggaaata
    14461 gctttgccct tcctcggtca gcaggagtga acgattacgg cggcggaaca gttttagccc
    14521 caaaaaatcc tcaagagact tgatttgatg acttactgcg gcctgggtca caaaaagctc
    14581 ttctgctgcg cgagtaaaac ttaaatggcg tgctgcggca tcaaaaactc gtaaggcatt
    14641 tagcggtggt aatcgtttag acatggcttt taaactttga tgttaaatga atttaacaat
    14701 tagatcacac tgtgtaacct attagttttt ttaatctgag ccattataaa ttgtccgttg
    14761 agcttctacc agcaaatacc tatagtggcg gcacttcctg agccggaacg aaaagtttta
    14821 tcggaatgcg tattctggtg aacttttggc ttacggttgt gatgttgtgt tgttgtgttt
    14881 gcaattggtc tgcgattcag accatggtag caaagctacc ttttttcact tcctgtacat
    14941 ttaccctgtc tgtccatagt gattaatgta gcaccgccta attgcggtgc ttttttttac
    15001 cttgcgatcg cgaattactg ttccagttcg accatctctt tcacgtcagt acggttgatc
    15061 tgctgtttgt tgccattagc gtctttatac gaaatcatac cgatatcgtt atcagtctgt
    15121 ggtttgccgt cagagacgat ggtacgtccg tcattggtgt gcatcacata gttcgaaccg
    15181 gaacaggcgc tcagggcaaa agtcagcata caggcagaaa taattgcggc agtctttttc
    15241 atcatcttct ccttaaaacg attaataaat cttaatagta ttgattaaca ggctaaaatt
    15301 aacgcctaac actattcagc atatgttact tacgcggctt cgccaggata tccagataat
    15361 tctgatggtt agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc ggtgcatcaa
    15421 gccgaggagt accatgaacg tttttaatcc cgcgcagttt cgcgcccagt ttcccgcact
    15481 acaggatgcg ggcgtctatc tcgacagcgc cgcgaccgcg cttaaacctg aagccgtggt
    15541 tgaagccact cgacagtttt acagcctgag cgccggaaac gtccatcgca gccagtttgc
    15601 cgaagcccaa cgcctgaccg cgcgttatga agctgcacga gagaaagtgg cgcaattact
    15661 gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc accactgaat ccatcaacat
    15721 ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc gatgagatta ttgtcagcgt
    15781 ggcagaacac cacgctaacc tcgtcccctg gctgatggtc gcccaacaaa ctagagccaa
    15841 agtggtgaaa ttgccgctta atgcgcagcg actgccggat gtcgatttgt tgccagagct
    15901 gattactccc cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtga ctggcggttg
    15961 cccggatctg gcgcgagcga ttacctttgc tcattcagcc gggatggtgg tgatggttga
    16021 tggtgctcag gggacggtgc atttccccgc ggatgttcag caactggata tcgatttcta
    16081 tgctttctct ggtcacaaac tgtatggccc gacgggtatc ggcgtgctgt atggcaaatc
    16141 agaactgctg gaagcgatgt cgccctggct gggtggcggc aaaatgattc acgaagtgag
    16201 ttttgacggt ttcaccactc aatctgcgcc gtggaaactg gaagcaggaa cgccaaatgt
    16261 cgctggcgtc ataggattaa gcgcggcgct ggaatggctg gcagattacg atatcaacca
    16321 ggccgaaagc tggagccgta gcttagcaac gcttgcagaa gaagcgctgg cgaaacgtcc
    16381 aggctttcgt tcattccgct gccaggattc cagcctgctg gcctttgatt ttgctggcgt
    16441 tcatcacagc gatatggtga cgctactggc ggagtacggt attgccttgc gggccgggca
    16501 acattgcgct cagccgctac tggcagaatt aggcgtaacc ggcacactgc gcgcctcatt
    16561 tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg accgcgcgct
    16621 ggaattattg gtggattaat gacaaacccg caattcgccg gacatccgtt cggcacaacc
    16681 gtaaccgcag aaacgttacg caataccttc gcaccgttgt cgcaatggga agataaatat
    16741 cgccagttga tcatgctggg gaaacagctt ccggctttgc cagacgagtt aaaagcgcag
    16801 gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg gatataccgt ggctgaaaac
    16861 ggcaaaatgc acttctttgg caacagcgaa gggcgcattg tgcgcggcct gttggcggtg
    16921 ttgttgactg ccgttgaggg gaaaaccgcc gccgaattac aggcacagtc gccgctggca
    16981 ttgtttgatg agctgggatt acgtgcgcag cttagcgcct cacgcagcca ggggttaaat
    17041 gcgttaagcg aggcgattat cgctgcgacg aagcaggttt aagcacagaa ccgggtcgga
    17101 taagacgttc gcgtctcatc cgacctgatt gtttaaccct gacgcgccgc tttcgccatc
    17161 atcttcttca gcgcatgaga aaccgcaaca aacccaaagg tggcggtcac catcgttgcc
    17221 gcgccaaatc cagatgcaca atccatccgc ttcggccctt ctgccgtggc tttcatcgca
    17281 cacaccgtac cgtctgactg cgggtacacc agcgcttcag tagaaaacac gcaatccacg
    17341 ccgagcttac ctttactgtt tttcactacg ccaaaatcgc ttttcaggcg ctcgcgcaac
    17401 ttcgccgcca gcggatcctg aatcgttttc gccagatcgg taacctgaat ctgcgtcggg
    17461 tcaatctgcc cacccgcgcc accggtcgtg accagcggga ttttattgcg ccgacaataa
    17521 gcaatcagcg ccgctttggg ccgtacacta tcaatggcat caatcacgta cgaataaccg
    17581 gcactcatat attgcgctac gttatccggc gtcacgaaat catccaccac cgttacacgg
    17641 cactccgggt taatctggcg aatgcgctcc gccatgactt ccgcttttgc cagcccgacg
    17701 ttatcgcgca gagcgtgaat ttgccgattg gtattggtga cgcacacatc atccatatcg
    17761 ataagcgtga ttgcgccaat ccccgtgcgc gccagcgctt ccgccgccca ggaaccgaca
    17821 ccaccgatac caaccacaca aatatgcgcg tcagcaaaca gttgcaacgc tttttcacca
    17881 tacagacgcg ctgtgccacc aaaacgctga cgccatgcat cactaattac cacagacata
    17941 gcacctcaga attaaaaagg gcgaagttat ccctcaccct aaacgcataa atgcctgatg
    18001 cgctacgctt atcaggccta cataactcct gcaatatttt ggatttgcat gatttgtagg
    18061 ccggataagg cgttcacgcc gcatccggta tgaacaacgc gcacttagtc aataatcagg
    18121 atgtgaggtt acccctcccc tgtcatatcc gtaaaaacgg catacagaat atcacatcag
    18181 ccgctaaaga cgttacctgc gcccggagcc gttttcagca cccaaacccg tccatagtgg
    18241 ttgtaccaac ctgcgcggtg tccggcttcc ggcccgatcc cttgatagat atcgaagtgt
    18301 tggcctttga ttgcgccccc gacatccaac gccaccatca gacgcaattc gtactgaccg
    18361 ttaaatttgc cgttattatc cagcagcggc acttctgcca gcaaggtagt acctggcgga
    18421 ataattgaac gatcggaggc aacagaagca cggccaacca gcggcaccgc acttgcccct
    18481 ttcaccggtg caaaagattg cggtttaaag aagacgaaag acgggttctg ttccagcagc
    18541 tcgcgaacct cggcttcact gtgtgtttcg ccccaatgac gaatcgcctg catcgacata
    18601 tcttcttttt tcacttcgcc acggtcgatc agcaccttac caatgctgcg ataggcatga
    18661 ccgtttttcc ctgcatagct gaaaaagtta agcggactgc catcaccaaa gtcgatatac
    18721 ccactaccct gcacatccat aatgaagtta tccatcagag agttgctgta agcgaggatg
    18781 tatttatcgc ccaatgcccc ggcgtagatc tccgcacgag acggcagacg accacgtttt
    18841 ggcggcatac ggtaaatagg atactggaac tcgccctggc gggtatggcg cgcctgaatt
    18901 accggcgtgt aatagcctgt aaactgcacg ttaccatagt tgtcggcacc ttccatctgc
    18961 caggcatcaa tgccgaactg gcgcatattg cgggtatcac cgcctgcgcg cagccactct
    19021 tgcaccgcgt tataaacatt actctggttg ccatacagac gcggtgacga attacggata
    19081 tggttaattt gctcggcaaa atcaccggcg ttaatcggcg cgccaacggc atctggctgg
    19141 ttcaccagag agaaaggctg ggtaaatttc ccgtctttat attgctgtcc gcgatcggtt
    19201 ggtttggaag agcaggcggc aagcattgcc acaaccgtgc ccataagaag gtactttacc
    19261 caacgtcctt tcattgttct cttcttcagg ttaaaaaata aggcgcaacg aagataacaa
    19321 accgccgcgt ttaatgaaat gagcagcctc tccctgacgc aaattttgca caaaaaatag
    19381 gctttagtgg tttgtttttg ttcaaaatca tgctaaatcc gtgatcgggg taaaaaaaag
    19441 gttgcatgaa aacgcgagcg gagtatagtg cgcatccacg gacgcggggt ggagcagcct
    19501 ggtagctcgt cgggctcata acccgaaggt cgtcggttca aatccggccc ccgcaaccaa
    19561 ttattgaaca ccctaacggg tgtttttttg tttctgatct cccataaaaa aagcgccatt
    19621 cagcgccttt ttatcatccc cttctcgcca gcgttgcccc atcggcaaaa tacgctttaa
    19681 tccccgcaag aatagactcc gcaacttcct gctggaaagt cgccgttttc agtttacgct
    19741 cttcctcaac gttactgata aacgccgttt cgaccagaat ggagggaata tctggtgcct
    19801 ttagtacggc aaacccggcc tgttcaactt gatttttatg cagcttgttg atttttccga
    19861 gcttattcag taccgcttta ccaaacttaa ggctgtcagc aatagtcagc gactgcacca
    19921 tatcgaacat ggtgtggtcg acatagcggt caccgctttt gctcacgcca ccaatcaagt
    19981 ccgaggcgtt ctgggtttgt gccagatatt ttgccgcagt actggttgcg cctttggttg
    20041 agagcgcaaa caccgaggaa ccgctcggct gacgactggt aaaggcgtcg gcatggatag
    20101 agacaaacag gtcagcacgc tgtttctggg cttttgctac gcgcacttgc aacggaatga
    20161 agatgtcttc attgcgcgtc atgtacacct tcatattgcc ctcttcctcg atcagagagc
    20221 gcaaacggcg agctatttgc aataccacat ctttttcgcg cgttttgtat ttccccaccg
    20281 caccggagtc ttcgccaccg tggccagggt caagcataat gacaatcgga cgatcgcgcc
    20341 ctgctttacc cggttgtgga ccactttgcg ctggcggcac ctgcttttcg aggtcgcctt
    20401 tgttgtaatc ctccagcagc gccagcagcg ggtcctgcat atcctgtgca ttggccggat
    20461 agaggtccat caccagacgc tctttaaacc cggcgaccgg cgcaagggca aacagctgcg
    20521 gttttacgtt ttgctttaat tcaaaaacca tgcgtacggt ttgcgggtca aattgcccga
    20581 cgcgcgccga cttgatgaac gggtcgtctg cacgaatttg tgccgccatc cccttgagta
    20641 ccgagttcag gtttacatct tcgatatcca ccaccacgcg ttcagggtta ctcaacgcga
    20701 actgcttata tttcagctga cgatttgatt ctaccgtcac gcgggtgtag ctggacgcag
    20761 gccagacgcg caccgccacg acctggctaa ccgcagccag gctgacctga cttacgctca
    20821 atagccacat agcgcccgcg ccttgcagta aacgacgacg gctgattgca gtgttggatc
    20881 ctgacatgcc tctcccgagc aaaagaaatc taagctgtgt aacaagtaaa cgactaattt
    20941 gaccggaaac tttagcgaaa gacgcataat ctgtcatcta ataaacggta aacattcttt
    21001 ttatattcac ggcattactg ataaaaaagt cgctctcgca taaaatttac acttgcaccc
    21061 tgcgaaaaaa cagaataaaa atacaataat ttcaaataat catgcaaagg ggtgtgccgt
    21121 ggtaaaggaa cgtaaaaccg agctggtcga gggattccgc cattcggttc cctatatcaa
    21181 tacccaccgg ggaaaaacgt ttgtcatcat gctcggcggt gaagccattg agcatgagaa
    21241 tttctccagt atcgttaatg atatcgggtt gttgcacagc ctcggcatcc gtctggtggt
    21301 ggtctatggc gcacgtccgc agatcgacgc aaatctggct gcgcatcacc acgaaccgct
    21361 gtatcacaag aatatacgtg tgaccgacgc caaaacactg gaactggtga agcaggctgc
    21421 gggaacattg caactggata ttactgctcg cctgtcaatg agtctcaata acacgccgct
    21481 gcagggcgcg catatcaacg tcgttagtgg caattttatt attgcccagc cgctgggcgt
    21541 cgatgacggc gtggattact gccatagcgg gcgtatccgg cggattgatg aagacgcgct
    21601 ccatcgtcaa ctggaaagcg gtgcaatagt gctaatgggg ccggtcgctg tttcagtcac
    21661 tggcgagagc tttaatctga cctcggaaga gattgccact caactagcca tcaaactgaa
    21721 agctgaaaag atgattggtt tttgctcttc ccagggcgtt actaatgacg acggtgatat
    21781 tgtctccgaa cttttcccta acgaagcgca agcacgggta gaagcccagg aagagaaagg
    21841 cgattacaac tccggtacgg tgcgcttttt gcgtggcgca gtgaaagcct gccgcagcgg
    21901 cgtgcgccgc tgtcatttaa tcagttatca ggaagatggc gcgctgttgc aagagttgtt
    21961 ctcacgcgac ggtatcggta cgcagattgt gatggaaagc gccgagcaga ttcgtcgcgc
    22021 aacaatcaac gatattggcg gtattctgga gttgattcgc ccgctggagc agcaaggtat
    22081 tctggtgcgc cgttctcgcg agcagctgga gatggaaatc gacaaattca ccattattca
    22141 gcgcgataac acgactattg cctgcgccgc gctctatccg ttcccggaag agaagattgg
    22201 ggaaatggcc tgtgtggcag ttcacccgga ttaccgcagt tcatcacggg gcgaggttct
    22261 gctggaacgc attgccgctc aggcgaagca gagcggctta agcaaattgt ttgtgctgac
    22321 cacgcgcagt attcactggt tccaggaacg tggatttacc ccggtggata ttgatttact
    22381 gcccgagagc aaaaagcagt tgtacaacta ccagcgtaaa tccaaagtgt tgatggcgga
    22441 tttagggtaa gcgatgaaaa tcaccggatg cggcaggcgt aacactcgtg cgtcgcatcc
    22501 ggcaattacg tttattcccg cgaactaaac aacgccgcca gaccactgcg ccgctcagta
    22561 cgagtggcga ttgccgcact taatatacgc tcatcggcat acagcgacag acgacgacgc
    22621 gcgcgggtca ccgcggtgta taccagctct cgcgttacta ccggcgtgcg ttggctcggt
    22681 aaaatcaacg ccgcatggtc gaactccgat ccctgcgatt tatgtaccgt catcgcccac
    22741 gtagtttcgt gctctggcaa gcgactcggt tgcacagact taatattgcc gtccggcatc
    22801 gcaaaccaga cgcgcgtccc ctgcccgcga tccagcgcaa taccgatatc gccattaaac
    22861 aacccaagcg cgctgtcatt acgggcaatc atcaccggtc ggccttcgta ccaacgagag
    22921 tgcggatgac gatgaatttt gcgcttctgt tgcataaact gttcaattcg ctcattcagt
    22981 ccagccacgc caaacggccc ttcccgcagg gcgcacaaaa gctggtactc attgaacgcc
    23041 tgaatgatta aatccggctc ggcacgcgct tgcagcagat ccagataacg tccgtaaccc
    23101 gcaagagctt cctcaagcat cgcaatataa tcttcgccgc tctgtaaaag tcgtttttcg
    23161 atatcagtaa aatcctgctg aaaaacggtt ttcactgccg ttttatcacc acggttgatc
    23221 gccgcagcta actgaccaat gccagaatcg ctgccgaaac gatagctttt ttgcagcagg
    23281 cagagactgt cgcgcaaaga tgccgcttct gtgccggttc ctgccggaac gtgagtcccc
    23341 gtcaggcggc ttagctgcct ggcacgctcg gcggtaaagc ccgcgttggc ataagcgcag
    23401 atatcgccca gcacagcccc agcctcaacc gaggccagtt gatcacgatc gccgagaaag
    23461 atcactcgcg catgatctgg caaggcgtcg atcagtctcg acatcatagg cagatcgatc
    23521 attgacgctt catctaccac cagcacatca agatgcagcg ggttaccggc atgatgacgt
    23581 aaacgctggc tacccggctg cgcgcccagc aatcggtgca aagtgctggc atcttccgga
    23641 atgcgtttct tttgttcatc ggtcagcggt aactgtcgca aagccttgcc gagagattcg
    23701 gttaagcgcg cggcagcttt acccgttggt gcagccagac ggatacggca gcgttcgccg
    23761 tcggccattt gaattaacgc tgccagcaac ttcgccacgg tggtcgtttt accggtgcct
    23821 gggccgccgg aaatcaccga gatccgccgc gtcagcgcca ctgccgctgc aactttttgc
    23881 cagttaattt catcgcttac tggaaaaagt ttgtccaggg tttgcgccag tagagcttca
    23941 tcaacctcaa tggtatgatt cacctcattg aagaagcgcg ctaccgtccg ctcgttacac
    24001 cacatgcgat tcaaataaag acgatcgcca cagaggatca tcggcgtggg ttcatctccc
    24061 ctgctgaccg cttgagaagc cagcaagcat tcttcccaat tttgtagctc accgatttca
    24121 ctgacacagg tcgccaacag cggattcgac gcctcgttat tttccagtcg tgaaagcggc
    24181 aaacagacgt gtccctctcc ggcatcatga cttaacagtg ccgccgcgag ggtgacggca
    24241 ggatgttcat ctcccgccac ggtcagggca aattgcacat ccagcgggcg tagctgtttg
    24301 tgctccacag cttccagtaa ttgcttttgc aatttcatta cgcctcctcc agggtcatac
    24361 cggcaaacat ctcatccatc agggcaatca acccggcgtt gggtcgggtt gtgtaaatcc
    24421 cttgttgcgg atgttcttta tcaacgccac gcaggaacag atagataacg ccgccaaagt
    24481 gaagatcata gtcgtaatca gcaatgcgat ggcgcagata acgatgcagc gccagggtat
    24541 aaagctgata ttgcagatca tagcggtgtg cctgcatcgc tgctgccata gcctgttggg
    24601 tgtaagccga actgtcttca cccagccagt tggatttata gtcgagcagg taataacgcc
    24661 cttcgtggcg gaataccagg tcgataaagc cttttaacat gccgcgtacc tgcatgaact
    24721 ccagcggcgg gcagccagcg gatagcgggt caaactggcg aattagtgca tcaagctggc
    24781 tggcgatgag cggttcgcta atcggcaggt aaaactccat ctccacctgt ttatcgcggt
    24841 cggaaagctg actcaggcta acacccgttt cattgagagg tgcctggagg acagccgtga
    24901 tccactcggt caacaccggt tcccactgcg attcaaagcc gccgagttcc agtttttcct
    24961 gtacccagtt cgggtcaacc ggctgggtaa aatcgaggtc ttcaaacaaa ctgtgcaaga
    25021 atgtccccgg tgacgcaccg cgcgggaact gatgcggtgt taacgtcggt tcttcaacga
    25081 cgctgaccac gcctgcggca tcaacatcca gccgtggcat cagatcctga gcgataccgt
    25141 gaccacgctg ctgcaagccg gagtagctgg tgacacgcca gttatcgccg ggcaatcgtt
    25201 gtagtgtcct cgcattcagt tctgcagtta aagcatcatt aacctgccag ggctggttat
    25261 caccagtttg tgccgtttgc caggcaatat catcatcgca taacgcttca atacaggtgc
    25321 gaagccctgc cgcatcttgc ggttcccctt tttgcagcaa acgcccgagc gcactttggt
    25381 ggacgtcggt gtcacctttt ttatcgccac gacggcgcac cagcggtgca acgccgagac
    25441 tgcaatgcca aaccgaacgc gtcagcgcca cgtaaagcaa acgcagatct tccgccagac
    25501 gttcgacctc cgcgaggtcg acgctttctg gcgcagcatt aagatccaaa actgcctcaa
    25561 acgagtggcg atcgtgataa aacgcctgat cctggacgcg gaaattggta ataaatggca
    25621 accagaccag cggatattcc agccctttcg atttatggat cgtgacaatc tgcaccagat
    25681 gtttatcact ttcgaggcgc atttgttggc tggaggcatt actgtctggc tcgaggatat
    25741 gttgcgataa ccagcgtacc agcgcatgtt cactttccag ctgcgttccg gcttcttgaa
    25801 gcagttcgct gatatgcaag atatcggtaa gacgccgctc accgcctgcc gttgccagca
    25861 agttttctgc aatgttacgc gccgacatca gcgcccgcag catcggcata acgccacgtt
    25921 tgcgccagat ttgccgataa ccatcgaact cttcgactac cgcatcccac gcatgttcgt
    25981 cattattcag cgtttcaata tccagcgcat tcagccccat cattgacgtt gccagcgcac
    26041 tgcgcagggt gttctcacgt tcgggcgtca tcaccgcctg caacaaccaa agcatttcct
    26101 gcgcttccag agtttcaaaa acactgtcgc ggttcgaaag gtaaacggaa gggatttcca
    26161 gcaacgttaa ggcatcgcgc acctgggcgg cctcctggcg gctgcgcacc agcacgctga
    26221 tgtccgaagc acgcaccgga cgcgcgtcgt cgccgttcat cagcaacgct tcgccccgct
    26281 gtccggcttg tagccagtcg cggatttgcg cagcacatac ctgcgccatg gtactttgat
    26341 agtcgccaac gccgcagctt tcgccttcca tcagccacat tttcatcgca ggctgcgttt
    26401 cacctttaaa tacaaaacgt aacgcctgat ttttcccggc tgatttcact ggaataaacg
    26461 gtatttcgcg aaacatgaac gtgtcatcag tctggctgaa aagcttgttc acactgttca
    26521 ccattccagg tgcggagcgc cagttggtgt ctaaagtgta gtgggcatga acttcactac
    26581 gcgccttcat ataagtgaag atatccgcac cccggaatgc atatatggcc tgcttcgggt
    26641 cgccaatcag caacaatgcg gtttccggct gatggtgcca gatacggcga aaaattcggt
    26701 actgctgggg gtcggtatcc tgaaattcat cgatcattgc caccgggaat cgcgtacgga
    26761 tcgccgctgc caacacctcg ccgctttcgc tacgcagcgc ggaatcgagc cgacttaaca
    26821 tgtcatcaaa acccaattcg ccacggcggc gtttttcacg cgctactgtt tcgcggatct
    26881 cagccaatgc gcgggtgatc aacagatcgc ggatcgacaa tggttctgca agcagttgat
    26941 cgatcgcctc aaacagtgga tgtcgcgggg tttccccccc ggccttcgtg cgatcttcta
    27001 agaaacgctg ggagaatttt tccagcgact ccggcaactg ataactgttt gtctcttctt
    27061 ctgcccaggc gctgatcttt tcgatccatt tagcctgatt gctacggtta aacttgcgtc
    27121 gatcaatacc agaagattcg atcagcgcat ccagttcacc cactgcgtcg cgccactgct
    27181 gttttaccgt atcaatacgc gccacaattt gcgcgtggcg ggaagccagc gtttcatcat
    27241 cgggcggcgg tgctttgata accggcggtt cgccttgcag ataacgatta atatcgcgca
    27301 gcaacgcctg cggccctttc caggtttcaa agacgacctg ggctatttca cgcggcagcg
    27361 ggtagcagtg gcgacgccag aaatcggcgc aggcctggta gcgtagcaga gactcatctt
    27421 caatcagctg ctgctcaaac agcatgccgg attcaaaggc attcaggttg agcatgcgct
    27481 ggcaaaagcc gtgaatagta aagactgccg cttcatccat ctgccgttcg gctaacaaca
    27541 accactgcgc ggcttgcgct ttatcgtcga tctcttccag caggcgttcg tacagtggat
    27601 tgtcggtggt ttcacgcaga caggcgatgc gcaactcgtg gatattgcta cggatacgac
    27661 cgcgcaattc tgccgtggca gcctcggtaa aggtgaccac cagcagttct tcaacgatca
    27721 gcgggcgggg aaaggcggcg gaaccgccta gtccaagtaa caggcgcaaa tagagcgccg
    27781 caatcgtaaa ggttttgcct gtgccggcag aggcttcaat caggcgctcg ccctgtaagg
    27841 gcaagcgcaa aggatctagt gtctcggcga catcactcat tcttttcact catcaggggc
    27901 attgtttgct gcaacgcgct gacgttctcc cacactttcc agccttcagg atgcacatat
    27961 tcggctttcc cgttctggct gccggaaatc tgcgacagaa tagccatgcc ttgcggctcg
    28021 accaccgcct gatggaagaa atcagcaagt ttttgcggcg tcagcagttt tatctgggcc
    28081 acgattttat cacgcgaatc gaagcgcata ttgccgcgat cgaaatcttt acttaacttc
    28141 gatgcttctt cgccgagcgt ttgcggtgcc tgcagcatct gggtaattac cgcctgctgg
    28201 atttgcgcaa actcatctgg cttcatcgct cgcaattttg cctctgcggt tgggaaaaac
    28261 gccttgtaac gctcccacaa gaatgaaggc tgtttatcat tgctttgcaa aaggaagccc
    28321 atgccccact gacgccccac gctcattgga aacgcaaaca cggcatagcc caattgttct
    28381 tcggtacgca actgattgta gaaccacggc tgtacgatct gccccaacag agagctatag
    28441 gctgagctgg tgtattcatc gtagccagtc ggtacaaata ccgctgccag tgcggagtcg
    28501 gtgctgttac cggctttttc aaagatgacg gattgttttt tatcgaccac tacatctttg
    28561 tttcgacacc actctgaacc atcagcgccc aactgttttt gcacatcgcg tgccagcgtt
    28621 gttgcctggg cctcggtcat gttgccgata accataaact ctggtcgagc ccctgatttt
    28681 aaggcgtcgc gataggccag cacctctttc aacgtaatgg agggcaaaat tttacgccgt
    28741 tcatctcgcg agaagtacgg cacttgcgag agcatctgcg cgggcataat cgcctgctca
    28801 aacgctttac ccttttctgc ggaatccatc atctggttat accaggactt cgcctgctca
    28861 agctgctctt ccgtagcggt atagctaaag tacccctcga gcaatgcctg gaacagctgc
    28921 ggcagacgct gggtgtaacc attagcatta atcataaggc cgttgttagc gttggtggaa
    28981 aaacttatgc caccaaccga cgcctggttg cttaactgat caagcgccag ccctgcgaga
    29041 taatcattga gcgcaaacat cacctgattg cgggcgctgt ccatggcttt cggattacgc
    29101 aaaatcaggc tgacatcagc tttgggctcg ctggcaaaat aacggcttgg cgcatacacc
    29161 acgcgcagat tcgactcatc aacaatcagc tctggatggt cgtatttctt ctctgactta
    29221 atcagcgaga aatcatcagg aatataaggg ttaagctctg gcaaagagag cgcaatgtcg
    29281 gcggcttttt tctgccagtc ggcgaaagtt tgtgcgctga ttttatcgac ctgatacggc
    29341 gcatcgacaa agtaagccgt tttgttgtgc ggctctttcg ggctgatata ccagatacgc
    29401 gcattctgcg gcgtcatcat cgccagacgt tcctttactg ctttagcatc gtaccgatcg
    29461 gcaatattga ctgcatccag cgtatgctca acaggaacgc gaatcatggt atctgccagc
    29521 cattcgacgt aatccatatc acgggtgatc gacggataac ggaagtcgat atccagcaca
    29581 ttcgccagtt catcgaagta ttgtttatca atgccttttt cacgtaacag attgagatag
    29641 ctaaaaattg ccgccacaac ctgatcgcga ttagccaggc ctttatcagt taaagacgca
    29701 gagatcgcta atacgccgct gttgccgttg acgataggat cggagttggc gctaatgccc
    29761 tcaactaatc cctgcttttg cagccagtca gaaagtgtac ctgggctgcg attgccaatc
    29821 agataggtaa tcaattcatc ggttttacta cggaacttcg ctgagttgtt atcgatgcga
    29881 aactcaacgc gcaacacttt acgcggcagc gcagggacgt aatgaatgat aatgcccttt
    29941 tgcgcgtcgg tgactaccgg cacggtgatt tccggttttt tgctctcttt gttcggcacg
    30001 cgaccaaagg tgtccgccgc catttttgcc aactccggca gcggtttatt actgtaaata
    30061 accgccttca tcaaattggc ggaatagtac ttctcgtgga aatctttcag cgcctgctgc
    30121 accggattac caggtttgtc gcttaaagtt tcgaggttac caccagaaaa ctttgaaccg
    30181 gggtgtgccg ggttaatggt ttctgcgctg acctgtgcca tgcgcatccc gtcacgcgta
    30241 cgcgccatgg ttaattcagc gttcactgca ttacgctcac gttcggcgta tttcttgtcg
    30301 agcaagggtt ctgcaatagc atcggccagg cggtctaccg caccgggcaa ggcgtcgttc
    30361 tcaacttcca gatagaaagc cgtgcgatac ggcgcagtgc tggcattgtg actaccgccg
    30421 tgcattttga gatattcggc cagactgtca gcctgcgggt actttttcga ccccatcaga
    30481 ctcatatgtt caaggtaatg tgccagcccc tggtacgcct cgggatcttc cagcgaccca
    30541 acgggcacta ccagcgccga gagcgattta actgcctgcg gatcagaaac cagcaagacc
    30601 accataccgt tatccagacg tatagcctga tactggcggt tatctttatc acttttacgg
    30661 atggtttcct gaatcggctg ccatcccgtt tctgcctgac ttaatggtgc ccagagggca
    30721 acaaacaaca ataatgcttt gaaccaggtg ctgcggggca ttcacggacc tcataatcaa
    30781 ctttattttc tggacagatt caacaggtta acgcaaatca tctgccagaa tttaatcttg
    30841 tgctgcacga gtcagcctat gtttatataa ccatcagtcc gtgactggtg cgcatcataa
    30901 agtaagcaga tagattgcgc aatttttata caacactcat gactgattaa agcgaaacag
    30961 cggtaacagg aaacgttgcg actgttcaac gatggcctcc attgtctctg gtgttaattg
    31021 ccgccagagc cgttgatacc agatatcatc accttcgcca cgcaccatca tgttgccttc
    31081 gtaagcctga aggaatttcg tacgggcttt ttgcaacgtg gaatcgtcat ccagcatggc
    31141 atcgttttgc gcgtcataac aggtttttag ccacgcgccg ccactttcag gtaacaccag
    31201 caatggcgcg gacattcctt cacgataccc ctcaatcagt tgtgagaggt aatgcaaagc
    31261 ctgttcggct gcaagcggcg gaaaacgcca ctcaccgtct ttgcgtagaa aaaggcgact
    31321 ttcgccatta ccaccgctgg cacagtagac aaggtgttcc agccaaagtt gcattccctg
    31381 cgccacactt aataaagagg gacgccagcg caacaggcca tccggctgca cctgcggcaa
    31441 ccagccagtt atctgcacac cgttgcaggc gagatcaatt tccatactct gccccggctg
    31501 gcgacaggca atgactctgt cggcaagctg ctgcatctcc tggcactgtg tttcccagaa
    31561 aatttcacca aaagcgccat acggtaaatc ccctgccgcc cggaagcggc ggaacaagcg
    31621 ttcggcatca tcctgctcaa ccagtgcatt caataactgc tgattgattt gataacggct
    31681 aagtccttcc agaataaatg gctcggtgtc tgggatttcg ctgtcttcag tatggaagtt
    31741 cacctgcaaa cgcatctgga agaacgcccg caccggatgt gcccagaatc gttgtagcgt
    31801 ttccagcggc acggtttccg gtaaggtaaa cggcagcggc tgaacaaatt cagaatgtgc
    31861 tttaccagcc tggctggccg caggtagcca ttcacgagca tagctttgtc gttcgcctgg
    31921 ctggtagttt tgcggatcaa acggcatccg ggtatggagg caagtaagat gcgcttttac
    31981 ccttgcctcg ctttcatcac agttgagcgc ttcatcgccc ggtagataat gactttgccc
    32041 gatgtagtcg atcagttcct gcaccagtac cgacgggaaa cgttcactgt tatcctgaat
    32101 ggaacgcccg atatagctga tatagagttt ttgctgcgcg gaaattaacg cttccaggaa
    32161 caggtagcgg tcgtcatcgc gacggctacg gtcgccacgc ttcggtttct ggctcatcag
    32221 gtcaaagccc aatggcgcaa gctgacgtgg ataaacgccg tcgttcattc ccagcaggca
    32281 aaccactttg aacggaattg aacgcattgg catcagagta caaatgttaa ccggtccggc
    32341 gagaaaacgc tggctgatac gttcttgatc cagacgctgt gccagttcat cacgcaatag
    32401 tgacagcggc accgcgtcgc catactgcgc acctaaacct tcggcgataa tcgcctgcca
    32461 ttgttgttcg atcagcgtca tcgccgcttc ggtttccgca tccggcagga agaaggcgtt
    32521 gagcatatcg cgacaaaccg gcaaccactc ttctagcgga cgctcctgtg ccagcccgcg
    32581 acgccagatg tttagctgca ttagcagtga agccagatgc cccaccagtt ctgcaattaa
    32641 gccgctcgat tcatcataag gtagaaccga ttgccactcg ccctgcgcgc tctccatcgc
    32701 gtagcccagc aacatacgcg tcaggccaaa tcgccaggtg tgttgtccgg tggcggggag
    32761 ttccagctcg cgaacgttgt cgtcatctat cccccaacga atgccggatt cgttgaccca
    32821 ctggcgtaaa taacgcagcc cttcttcggt gatgtcaaac cgcgccgcca gcaccggcac
    32881 atccagcagc gccagcacat cctctgacac aaagcgactg tcaggcagtg ataacaggct
    32941 gataaacgct tccagtaccg gatgtgactg ccgcgcacga cggtcggaaa tggcgtaagg
    33001 caggtaacga tccgcaggtg cgctaccaaa cactgcctga ataaacggac tgtagctgtc
    33061 gatatcagcc accatcacga tgatgtcgcg cggagtaagt gtcggggctt cctccagcat
    33121 cgccagcaag cgatcgtgta aaacttcaac ttcacgctgc gggctatggc aaacgtggaa
    33181 tgtgatactg ctatccagtg gatcaagcgg gcgtttgtta tcgctacggg aaaactcttc
    33241 gatgttcaca cccgcaacgg cgcggttttc cagttccaga atgtcagact gaatgttatg
    33301 cagcaggtta tctggcgtca catcgacaaa agcgtccagc tcctggctgc tctccaggtc
    33361 agaaaggaga taaatgtagt cgcgcccaag cttcccccat gaggccagca gcgagttgcc
    33421 gacatcctgt tcaccatcgc tgttaaagag ctgtccggca ttttcgctat cgcgaaatag
    33481 cggcaattcg cgatcttcaa aactgtgtcg acgctgacga gtcagcagtt tcgccagata
    33541 agcaggatct ttaatatcgc cccagtaata acggcagggg ttggtaaaca ggagatggat
    33601 ttcaatatgt ttacccagcg cctggagcgc ctggagataa acaggcggta acgcggaaat
    33661 accgcatata aagacgcgcg aaggtaaccc cggcgggcag gtcgtcgcgg actccagcgt
    33721 ttcgataaag cgctgataga gattcgcgcg gtgccagcgc ggttgtccga gttcatgggt
    33781 atattccacc agcgccttcc acaacggggc ttgccaggcc tgtgcttctc ccagcccttc
    33841 aaccaaatgt cctgtttccc actgtgccag ccagtccgga cgatagacca gatactggtc
    33901 aaacaggtcc gccgcttttg aggaaagctg gaacagtttt cgcttgtcgc tatcgtcagt
    33961 cagataatgc cgcaacaggg taaagtcttc gcgctccagc aattgcggca gcagagtcat
    34021 cagtttccag ctcatgctct gtttgttaaa ggcgctctct ttggggattt ccggtaacac
    34081 ccggacgaac atatcccaga taaagctcgc tggcagcgga aaatcaatgt ttgccgcaat
    34141 accaaacttt tgcgacaggg tcatttgcag ccactgcgcc ataccggtac tttgcaccag
    34201 aatcatctct ggttcgaaag gatcgtccag ccgttcgcgt tcgacaataa actccatcaa
    34261 cgcttccagc acgtccagac gattggaatg gtagaccctt aacatagcgg ctcctgacta
    34321 ctgacaattc gggcaatgca ggcgggtcat ctcgccttct ctgcccccgg gtgaaactag
    34381 cgtaacgctg atgctgacac atcccgcctg cgatgtctgc attcggttga cctgccagtt
    34441 ggcaggtggc gaaatcgcgc gcagttgcgt ttgctgccaa ccatgccgcc agagcttttg
    34501 gtactggttt ctgctggcga gactgttcat tgatgttcgc tggatacccg ataacgcagt
    34561 gacaatcatc accatcagca ccatcagcac catcagcacc atcagcacca tcgcaaccat
    34621 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa tgccccctct
    34681 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatttttcc atcaatgact
    34741 tcgcctgttc gccacaacga aacgccttca taaccggcaa tcaataaggc ttcattatct
    34801 gccagtaaac gcaaacaaac ctgggcatcg gttccagcgt actgcgagca ctgaactgct
    34861 ggctgcgtct gccaggagtg catttttccc cacgccagcg ccgactgaac gatggcctgg
    34921 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    34981 attccttgca atagcaagct acccaaaacc agcagcatca ggaccagagc cagtgacgaa
    35041 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    35101 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    35161 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    35221 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    35281 tcagacgaaa tccaatctgg tcggactctt ttaccggttc gcgatcccag ataccgttac
    35341 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    35401 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    35461 gcagccagat ttcatcttcc agtgccagct tacgagtgct cgttaaactt tcacgctgta
    35521 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    35581 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    35641 ataaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    35701 gaagcgaata tgcacggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    35761 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    35821 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    35881 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    35941 ccgcgcctag ctggcggttt gccaaagccg ttgcgactgc tgccagtatt gccagccata
    36001 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    36061 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    36121 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgcaaaa
    36181 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaccgacgc acactggcgt
    36241 cggctctggc aggatgtttc gtaattagat agccaccggc gctttaacgc ccggatgtgg
    36301 atcgtagcct tcaatctcaa agtcttcgaa acggtagtcg aagatggatt cgggtttacg
    36361 tttgataatc aacttcggca gcggacgcgg ttcgcggctt aattgcagat gagtttgatc
    36421 catatggttg ctgtacaggt gcgtatcgcc accggtccag acaaaatcac ccacttccag
    36481 atcgcactgc tgcgccatca tatgcaccag taacgcgtag ctggcaatgt tgaacggcag
    36541 gccgaggaag acgtcacagg agcgctgata aagctggcaa gagagtttgc cgtctgccac
    36601 atagaactgg aagaatgcat ggcacggtgc cagcgccatt ttatccagtt cgcctacgtt
    36661 ccacgctgaa acaataatgc ggcgcgaatc cgggtcgttt ttcagctggt tcagtaccgt
    36721 agtaatctgg tcaatatgac gaccatctgg cgttggccag gcgcgccact gtttaccata
    36781 cactggcccg aggtcgccat tttcatcggc ccattcgtcc cagatggtga cattgttttc
    36841 gtgtagataa gcaatgttag tatcgccctg caggaaccac agcagttcgt ggatgatgga
    36901 acgcaggtgg caacgtttgg ttgtcaccag cgggaatcca tcctgcaggt taaaacgcat
    36961 ctgatgacca aaaatggaaa gcgttccggt tccggtacgg tcgtttttct gtgtgccttc
    37021 gtcgagcact ttttgcatca gttctaaata ctgtttcatg gttcctcagg aaacgtgttg
    37081 ctgtgggctg cgacgatatg cccagaccat catgatcaca cccgcgacaa tcatcgggat
    37141 ggaaagaatt tgccccatgc tgatgtactg cacccaggca ccggtaaact gcgcgtcggg
    37201 ctggcggaaa aactcaacaa tgatgcgaaa cgcgccgtag ccaatcagga acaagcctga
    37261 gacagctccc attgggcgcg gtttacgaat atacaggttg aggataataa acagcaccac
    37321 accttccagc agcagttcgt aaagctgtga tgggtggcgc ggcagcacac cgtaagtgtc
    37381 gaaaatggat tgccactgcg ggttggtttg cagcagcaaa atatcttctg tacgggagcc
    37441 tgggaacagc atggcaaacg ggaagttcgg gtcaacgcgg ccccacaatt caccgttaat
    37501 aaagttgccc agacgcccgg caccaagacc aaacggaatg agtggtgcga taaaatcaga
    37561 gacctggaag aaggaacgtt tagtacggcg ggcgaagata atcatcacca cgataacgcc
    37621 aatcaggccg ccgtggaaag acatgccgcc gtcccagaca cggaacagat acagcggatc
    37681 ggccataaac tgcgggaaat tgtagaacag aacataacca atacgtcccc cgaggaagac
    37741 gccgaggaag cccgcataga gtaagttttc aacttcattt ttggtccagc cactgcccgg
    37801 acgattcgcc cgacgtgttg ccagccacat tgcaaaaatg aaccccacca gatacatcag
    37861 gccgtaccag tgaagcgcca cgggtcctat tgagaaaatg accggatcaa actccggaaa
    37921 atgcagatag ctactggtca tctgtcacca caagttcttg ttatttcgct gaaagagaac
    37981 agcgattgaa atgcgcgccg caggtttcag gcgctccaaa ggtgcgaata atagcacaag
    38041 gggacctggc tggttgccgg ataccgttaa aagatatgta tatgatccgc gctataaccc
    38101 tccgcgaatc agcccgccca tgccgcgacg ctccataaag gctgcaacct gatgacgaac
    38161 ttcggtcgcc agttgcgctt ccagactacg ctgcgcaaga ttttctgctt cggcaaaatc
    38221 aatgcgccgc agcaggtatt ttacccgcgc tacagaacgt ccgttcatag acagatggcg
    38281 atacccaagc ccaatgagga ttgccacgca catgggatcg cccgccattt caccgcacaa
    38341 acgaagatcg ataccatgta tttccgcttc ccgggcgatc atcgccagag ctcgtaacat
    38401 tgcaggatga agactgtcgt aaatgttcgc cacccgggta ttgttgcgat caacggccag
    38461 aatgtattga gtcagatcgt tggtgccaac agagatgaaa tcgaccctct ttgccagatg
    38521 cggcagcata aataccattg acggcacttc cagcatgatg ccgatacgtg gtttgggaat
    38581 ttcgtaaccg atcatctcct cgacttcacg tccggcacgt tcaatcaggc ggcgtgcttc
    38641 gtcaacttca tcgaggctgg tgaccatcgg caacagaata ttcaggttgc ccgtagcggc
    38701 attagcacgc agcatcgccc gcacctggat caagaagatc tccggctgat cgagcgtaat
    38761 gcgaatccca cgccaaccca ggcatggatt ctcttcgctg atcggcatgt aaggcagctg
    38821 cttatctgct ccgacatcca gcgtacgcaa ggtgacgggt ttatcattga acatttgcag
    38881 catcccctga tactgcgcca cctgttcttc ttccgacggg aaaccacttt gcagcatgaa
    38941 tgggatttca gtgcgataaa gaccgatgcc atcaatacag ctgcccagtt tttcttcatg
    39001 ttccgggctt aaaccagcat tgagcatgac ttttatacgc tcaccgcttt ttaactgcgc
    39061 gggtaaattg acgtcatctt ccgccagacg gctaagctca atctcttcac taattagccg
    39121 ctgatattct tgcagcagta ccggctccgg atcgaccagc aattcaccgc gatagccatc
    39181 aacgatcagc gtcctacgat gcagcaccga aggctgaata tccgcgccca tcacggtagg
    39241 gatccccagc gcacgtacca tgatcgcagc atgggagttg gctgcgccat ctcgcacgac
    39301 aacaccgact aagcgatcct ggggcagctc agcaagagtt gtcgctgaca gttcatctgc
    39361 caccagaatg aaacgttccg gccaggcgtt cggcccttga ttagcgtcat caagatgaaa
    39421 cagcaatcgc tgacccagcg cacgtaaatc accagcccgc tctttgagat agttatcgct
    39481 tagcgcggca aactgttcgg caaatttttc aatgaccgtt tttaccgccc actctgccac
    39541 cgagccttta tcaacctcgg caaacaattc gcgacgcagc cgggtgtccg aaagcaggtg
    39601 cgagtaaaga tcgaaaatag ccgccgtttc tttttgtgct ccggcggcaa agcgtttgct
    39661 gtagcggcga aactcgtttg ccgcttcttc cagcgccccg gtcagtcgtt cgcgttccag
    39721 agccggatcc agcgttgatg cctgatacac ctgttccatt aaaggtaacg tggcatcctg
    39781 ccagccttcg gcaatcgcca caccaggtgc tgccggtaat gcgcggattc gcgtctggcg
    39841 atattgccca aacagggcag tcaactgcga ctgagaaaga atagctgcca tctgggtggc
    39901 aagcgtcacc aggaaggatt cttcactttc gtcatactgg cgcaactctc gttgctgtac
    39961 caccagtaca ccaagcaact ggcgacgttg aataattggt acgcctaaaa acgcgcggaa
    40021 acgttcttct tttacggagg ggatgtattt gaagctgggg tgcttttgcg catctgcaag
    40081 gtttatcggt tccgccagcc tgccaaccag gccgacgatc ccttcatcaa acgcgagcgt
    40141 tacagtgcga ccgcgtggtt ttttcagccc ccgggtcgcc atcaggtagt aacaacgtcg
    40201 atcatgatcg gccaggtaga ccgaacagac ctcggtatcc atcgcaagac agatgtcggt
    40261 aaccagaata tttaacgcct cattcaggcg tggtgcgctg gctacctttt cgactatttc
    40321 gcgcaggcga gtgagcataa ttggcgtgac ttaacctctt ttacgtcgat aagcagatgc
    40381 gttttgtggt tttggcgtat tttcctgcag tgacatcacc acactcgcga actctttcat
    40441 taccctacgg tagacatcac gtttaaatga caccacctgt ctgaccggat accagtaact
    40501 tacccatcgc cagccgtcaa actctggtgt actgctggtt tgcatattga tttctgcatc
    40561 gccgcttacc agctgcaaga gaaaccattt ttgtttttgg ccgatacaaa ccggcttcgt
    40621 gtcccaacgc accaaacgtt tcggtaattt gtagcgcaac cagttacgcg ttgaagcaag
    40681 gattcgaacg tctttgcggc ttaatcctac ttcttcaaac aattcacggt acatcgcctg
    40741 ctctgcggat tctccggggt tgattccgcc ttgcggaaat tgccaggagt gctgaccaaa
    40801 tcgccgggcc cacattacct gcccctggcg attacaaatc acgataccta cgtttgggcg
    40861 gtagccatcg tcatcaatca ccggactacc tcaaaataaa gctttatata cgaatgattg
    40921 tttcatactc caggaagacg gtaaaccact ctctgcaggg cattacacac taataacaat
    40981 tgaataactc acagttatgt gcagagttat aaacagagga aggggtggat agccgttttt
    41041 attcaccttt tctgtggata gagttgtgaa gaactacgga attactacgg gaaaacccgg
    41101 agcattctga ataagcccga tatagacatt ttaaaatatt cattattagt caatgcatta
    41161 catgtaattt gagtaatgaa aaagagtaat tcgtgaccca ggtcacacct ctcatttacg
    41221 ggttggcgaa agatcaacca atgccgtgtt tatccacaga atgtgccact aagttaagca
    41281 ctgaaccact aaaaactgga gtttcgtcgc acgtcaaggc tgtaaatgaa aacagtagtg
    41341 gaggtttttc acagttatcc cagctttctg tggataacat ggtgtaagat cctgtttatt
    41401 ttcagtgacc agatttggaa aacctattgc agtgttgcgc aactcgatta ccggcagcct
    41461 aaaaagcaat ataaatcaga gaattgaaca acacatgtgg aaaaagttac actgcgaata
    41521 ttcggcacat aattgctgtt tgttttttaa ccaaggtatc atgacatgtc ccaacctcgc
    41581 ccactgctct ctcctcccga aactgaagaa cagttgttag cgcaagcaca gcaactttct
    41641 ggttatacat tgggagaact ggcggcactt gacgggctgg ttacgccaga gaatttaaaa
    41701 cgcgataagg gctggattgg cgtgttactg gagatctggc taggtgccag cgcagggagt
    41761 aaacctgagc aagattttgc tgctctgggc gtggaactta aaactatccc tgtggatagt
    41821 cttggtcgtc cgctggaaac aacattcgtt tgtgttgccc ggttaacggg taatagcggg
    41881 gtgacctggg aaaccagcca tgtgcgccac aagctcaaac gcgtactgtg gataccggtt
    41941 gaaggcgagc gcagcatccc gctggcgcag cgtcgcgtag gatcaccgtt gctgtggagc
    42001 ccgaatgaag aggaagaccg acagctacgc gaagactggg aagaattaat ggatatgatt
    42061 gttctcggtc aggttgagcg gatcaccgct cgtcacgggg aatatttaca gataagaccg
    42121 aaagcagcga atgcgaaagc gcttaccgaa gccattggtg cccggggcga acggattctg
    42181 acgctgccac gcggctttta tttgaagaag aatttcacca gtgcactact ggcccgtcat
    42241 tttctgatcc agtagccatc gctttgacct gccgctttcc gggcatataa ttaccgcttc
    42301 atttttttgg cagggctttt tagatgttat ttgcatggat aaccgatcct aacgcttggc
    42361 ttgcgctcgg tacgctgacg ctgctggaga tcgttcttgg gatcgacaat attattttcc
    42421 tttctctggt ggtggcaaag cttcccacag cacaacgtgc tcatgcgcgc cgtctggggt
    42481 tggcgggagc catggttatg cgtctggcgc tgctggcatc aatctcttgg gttacgcgcc
    42541 tgacaaatcc gctttttaca atattcagtc aggaaatttc tgcccgtgat ttgattctgc
    42601 ttctgggtgg cttgttcctt atctggaaag ccagcaagga aatccacgaa tccattgaag
    42661 gtgaagaaga agggctgaaa acacgcgttt catcattcct cggcgctatc gtgcagatta
    42721 tgctgctgga tattatcttc agcctcgact cggtgattac cgctgtgggt ctgtcagatc
    42781 acctgtttat tatgatggca gccgtggtga ttgccgtagg cgtgatgatg ttcgccgcgc
    42841 gctcgattgg tgattttgtc gaacgccatc cttcggtaaa aatgctggcg ctctctttcc
    42901 tgattctggt gggctttacc ctgattctgg aaagtttcga catccacgta ccgaaaggtt
    42961 acatctactt cgcgatgttc ttctctattg cggttgaaag cctcaacttg attcgcaaca
    43021 aaaagaatcc gctctgatac tccgtacgct ctcctgcggg agggcgtact cactcctttt
    43081 accctcacag tttaagattt tcctgctttc aagatatata acgtcggttt ataaacagac
    43141 tattatcata ggtaggccaa atgaggataa ccggatgaaa aaatgggcag taataatttc
    43201 tgcagtcgga ctggcgtttg ctgtttccgg gtgttccagt gattacgtca tggcgaccaa
    43261 agatggccgt atgattttga ccgatggaaa acctgaaatt gatgatgata ccgggctggt
    43321 gagttatcac gatcagcaag gtaacgcgat gcaaattaac cgtgatgatg tttcgcaaat
    43381 tattgaacgt taacaaataa ggtcagcatc cggctggcct taagattttt ctctttccct
    43441 tttcccttcc ctctgccatt tttatattcc ttatgtcgtg attataaaaa ggaaacggct
    43501 atgcaatatc accgtatacc ccacagttcg ctggaagtca gcacgctggg gcttggcacg
    43561 atgacgtttg gtgaacagaa cagcgaagcc gacgcccacg cacaactcga ctatgccgtc
    43621 gctcagggca ttaaccttat cgacgttgcc gaaatgtacc cagtacctcc gcgccccgaa
    43681 acgcaagggt taaccgaaac ctacgtcggc aactggctgg cgaaacatgg cagccgcgaa
    43741 aagttaatta tcgcctccaa agtgagcgga ccgtcgcgca ataatgacaa gggcatccgc
    43801 ccggatcagg cgctggatcg gaagaatatc cgcgaagcgc tgcatgacag cctcaagcgc
    43861 ctacagactg attacctcga tctttatcag gtgcactggc cgcagcgccc gaccaactgc
    43921 ttcggcaaac tcggttatag ctggacggat tctgcgcctg cggtttcgct gctggatacg
    43981 ctggacgcac tggcagagta ccaacgcgcg ggaaaaattc gttatatcgg cgtgtcgaac
    44041 gaaactgcat ttggcgtaat gcgctacctg catctggcgg acaaacacga tctgccgcgt
    44101 attgtcacca ttcagaaccc ttacagtctg ttaaaccgca gttttgaagt aggtctggca
    44161 gaagtcagcc agtatgaagg ggtcgaactg ctggcctatt cgtgcctggg tttcggcacg
    44221 ctgaccggga aatatctcaa tggtgcaaaa cccgctggcg cacgtaatac gctctttagt
    44281 cggttcaccc gctatagcgg tgagcaaacg caaaaagccg tcgcggcgta tgttgatatc
    44341 gccagacgtc atggccttga tcctgctcag atggcgctcg cgtttgtacg ccgtcaaccg
    44401 tttgttgcca gcactctgct gggcgcaacc acgatggatc agctgaaaac taacatcgaa
    44461 agtttgcatc tggagttaag cgaagacgta ttagctgaaa ttgaagcggt acatcaggtt
    44521 tatacttatc ctgcaccata aagattaacg ccggttaaaa ccggcgttaa atattaatga
    44581 cggcgctgcc agatccacag cgccgttatt gccagcgcaa atagcgcacc gaagccaatg
    44641 ccaatgggca cgaccgggat gcctaccatt accgccagcg agtaaatgcc cagcatcaac
    44701 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    44761 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccagcacg
    44821 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga gaaaatcggt
    44881 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaacc
    44941 agcttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    45001 gtcggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    45061 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    45121 ccattgcacc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    45181 caggactgcc ccggacgcgc cgccagtttg ggaatgtaga tattggcaac gaccgcacca
    45241 ccgtaggcca gtgcgcatgc ggccagagcg accaggacat gccagtcagc cagcacacca
    45301 ccggctacgg aaccgagtaa aatcgccgct atggcagaag cttccattaa accgttagct
    45361 tttactaact tactacccgt ggttaattcg ccgagaatac cgtatttcgc cggtgaatag
    45421 gctgcagcac caacacccac cagcgtatag ccgagaaacg gattgatacc aaagcagata
    45481 ctggctgcgc ccagcagctt caggccgttg gcaaacatca tcacccggcc tttggcgaag
    45541 ctatccgcca cctgcccgac aaacggcgcc aaaagaatgt aagcacctac aaacaccatt
    45601 tgcaggatgg gctggctcca ctccggatag aactgcgctt tcagtaacgc cagagtggca
    45661 aacagtagcg cgttatcgcc aaacgcagag agaaactgcg ccacgataac cgctttcatc
    45721 cccttcgacc acaacgaagt gttagtgtgc actgactcac tcatcgtgtt gttccgcttc
    45781 gtctacccag cttttcaacg tgacaaagtc aggtttgccg ctgccaagta atggcatctg
    45841 tttcagatag cgaatatcgc gcggtacagc aagctccggc acgccgtgct cgcgggcata
    45901 ctgttgcaac ttatcgcgcg tcagttcgtt atctgtggtg aaaagcacca gtgcctcgcc
    45961 tttgctggca tcgctcttaa tcgcagtggc atggacttta tctggcgaaa caccaagtgc
    46021 cagttgttcc accatttcca gcgacaccat ttcgcctgca attttggcaa agcgttttgc
    46081 gcggccctga atctgcacaa agccctgctc gtcaaaacgc acaatatcgc cagtgtcata
    46141 ccagccgcgc tccatttcgc cgcgaacatt ctcggcggtg ggcacttcca gtacacctgg
    46201 cttctccacc cgcagatagc cgttcattat gttcggccct ttcagttgca ggcgtccgcc
    46261 ctcttcgata ccagggaccg acaacaggcg tgcatccatt cctggtagaa tacgccctac
    46321 cgtaccgggt ttcgccgcca tcggtacgtt gatagaaacg acaggcgcgc attcggtcac
    46381 accgtagcct tcaaggatgc gcaggccaaa tttatcctgc caaagctgtt tggtactttc
    46441 ttgtagtttt tctgcgcctg ccaccacata gcgtagacga tagaagtcat acgggttggc
    46501 gaaacgcgcg tagtgaccga ggaaagtcga ggtaccgaac aacacggtgc aactgcggtc
    46561 atacaccaag tccggcacaa tgcggtaatg cagcgggctt ggataaagga acacttctgc
    46621 acctgtaagc agtggcgtaa acaggcctac cgtcagccca aaggagtgaa acagcggtaa
    46681 tgccgacata aagcgatcgt tggtggtgaa gtcggcaatc gttttaatct gctcgacgtt
    46741 cgccagaatg cttttatggc tatggacgac gccttttgga tggccttcag aaccggaggt
    46801 aaagaggatc agcgcctctt cttccggctg ctgttttacc tgcgccaggc gcggcatcag
    46861 caaatgagcg aagatccata ctttgtcggc agtggtgaca tctgctttta aatcttccag
    46921 atagacccag cgcacctgag taagttgctc cggcagatgc cagagtttgc ctttatcgag
    46981 aaactggcgg gaagtgaaga tggttttgat ttcagccgcc gtaatagcac tggtcagccc
    47041 ttttaccccg gcagtgtagt tcatcattgc gggaatgcgg cgacgcgcga tggccccaaa
    47101 aatcactgcc gcactgatgc ctgcattggg cagcattaag ccgatgcgtt cgccttcaac
    47161 actgtatttt tcaaggatgc gtccaacaaa cagcgttttc gtaagcaatt tgcgatagga
    47221 gtctggggta aagttgacgt cttcgacaca tttcttcccg gctccgaagc ggtacattgc
    47281 actcagtaaa gattcgtaca gagtttcacg cgggcgcacc gccatacgcg cttccatcat
    47341 tatttgatgc agcatttcgc cagcgatttt gcgacggtca cgggcacgcg gcgcatccgg
    47401 catcgccacc tgcgttggag gcaaaatatg cagagtaatt tgcgggaaca agcgacgttt
    47461 aaccagacct ttcaggcggc tgaagtgcgt aagttccgcc ccttcaatac gcacaggaat
    47521 aaccgttgca ccagacttcg ccgcgacaaa acccgcgcca tcgtagattt tcatcagcga
    47581 gcctgtagtg gtgatgcgtc cttcagggaa aatcaccact ggtcggccct gttccaccag
    47641 acgtaccaga tgtttaatag ccataggttg cgtcgggtcg agaggaacaa agtcgataaa
    47701 tgatttcagc caacgcatat accactgttg gcttattgag gtgtaaacgg caaacactgg
    47761 acgcacaggt aaaaacagtc caagcaaaat gccatcaata aaagagacgt gattaggcgt
    47821 aattagtacg cgctcgccct tcagtgcctg ggtgtcaccc gtaacgcgaa cgcgatacaa
    47881 aacacggcac aaatttcgaa aaaagctaaa aagcattcca actccctttg ctctgattca
    47941 gtaaaagcga atggagggag attacacgag ataaagaacg cgagcgacag taaattaggt
    48001 gcgaaaaaaa acctgcgcat ccgcgcaggt tggtgcaaga gacagggtac gaagagcgta
    48061 ccgaataatc tcaccaatca atacctctgg gatcttgatt gtggtctgca cgacgactct
    48121 tcgccagcga gaaaacgcaa aggaatgaag ggaaatgcaa cgaggtgtgt aaattgtcgg
    48181 ttactgttac agattgatga ccggcaaaaa aaaacctgcg catctgcgca ggctggtgta
    48241 attcatgtgc tcaacacgaa gttgacttca cctatcaata cctctgggat caccacttta
    48301 gcaacctgaa gccaaacgcc accagcggtc aatcgcaaca gcgtttcgca aagtgtaacc
    48361 aaaggtttgc aatctctttt tttgtcttac ttatttgcgt ttggctcaca ttcccacgat
    48421 gaaaacacgc caccccttga accaacgggc gttttccgta acactgaaac aatgtaagcg
    48481 tttacccact aaggtatttt catggcgacc ataaaggatg tagcccgact ggcaggcgtt
    48541 tcagtcgcca ccgtttcccg cgtcattaat aattcaccca aagccagcga agcttcccgg
    48601 ctggctgtgc atagtgcaat ggagtctctt agctatcacc cgaacgccaa cgcccgtgcg
    48661 ctggcgcagc agaccactga aacggtcggt ctgatcgttg gtgatgtttc cgatccgttt
    48721 ttcggtgcaa tggtgaaagc ggtcgaacag gtggcttatc acaccggtaa tttcttattg
    48781 attggcaacg gttaccacaa cgaacaaaaa gagcgtcagg ctattgagca actgatccgc
    48841 catcgctgtg ctgcgttggt cgtccatgcc aaaatgatcc cagatgccga tttagcctca
    48901 ttaatgaaac aaatgcccgg tatggtgctg atcaaccgta tcctgcctgg ctttgaaaac
    48961 cgttgtattg ctcttgacga tcgttacggt gcctggctgg caacgcgtca tttaattcag
    49021 caaggtcata cccgcattgg ttatctgtgc tctaaccact ctatttctga cgccgaagat
    49081 cgtctgcaag ggtattacga tgcccttgct gaaagtggta ttccggccaa tgatcggctg
    49141 gtgacatttg gcgaaccaga cgaaagcggc ggcgaacagg caatgaccga gcttttggga
    49201 cgaggaagaa atttcactgc ggtagcctgt tataacgatt caatggcggc gggcgcgatg
    49261 ggcgttctca atgataatgg tattgatgta ccgggtgaga tttcgttaat tggctttgat
    49321 gatgtgctgg tgtcacgcta tgtgcgtccg cgcctgacca ccgtgcgtta cccaatcgta
    49381 acgatggcga cccaggctgc cgaactggct ttggcactgg cggataatcg ccctctcccg
    49441 gaaattacta atgtatttag tccgacgctg gtacgtcgtc attcagtgtc aactccgtcg
    49501 ctggaggcaa gtcatcatgc aaccagcgac taaccgcagt taaagcaatt ccagcgccag
    49561 taattcttcg atggtctggc gacggcgaat caaccgcgcc tgaccattat caaacagaac
    49621 ttctggtaac agcggacggc tattgtagtt ggatgacatt gatgcgccat atgcccctgt
    49681 atcatgcagt accagataat cacctgcctt cacttccggc aaggcgcggg tttcaacatt
    49741 tcccccttcc tgctgggtaa agacatcgcc cgattcacat aacggcccgg cgacgacggt
    49801 ttccaccgtt ggcgcgtgtt ccagagaacg accatcagct gccagggcac tgatatggtg
    49861 gtaactaccg tacattgccg ggcgcatcag atcgttgaac ccggcatcaa ccagcacaaa
    49921 gtggcggcta cccatttgtt tgacgctccg cacctgagta attaatacgc cagactgcgc
    49981 taccaggaag cgacccggtt caatttccag tttcacaggg tggcccaaat ggcgagcgat
    50041 ttgctcacgc gcggcattcc acagaccata ataatgttcg gtatcaaccg cctcttcacc
    50101 ctgttgataa ggaatagaaa gcccaccgcc cgcagaaata gcctgtaaat cctgaccgaa
    50161 ttcgataacc tgacgcacca tagcaccaca cacctgctcc agatgggcat aatcaacgcc
    50221 agaaccaatg tgcatgtgaa tgccgactag ctgcagatga tgacgttgta tcacgtccag
    50281 tgcggcgggc agatcggtgt accagatacc gtgcttgctg ttttcgccac cggtattggt
    50341 tttttggcta tgtccgtgac caaaccccgg attaacgcgc agccataccc gatgccctgg
    50401 cgaaacctgg cccagttggt cgagcgtatc aacagaaccc gcattcaccg gaatttgcaa
    50461 ttcactgacg cgttcaagcg tcgcctgatc gataacatct gccgtaaaaa caatatcatc
    50521 ggggtgcgtt tgcggattgt aacccgccgc caacgcacgc tctatttcgc ctaacgagac
    50581 ggaatccact ttcacgccct gctcacgcat taagcgcaaa atatgaatat tggaacaggc
    50641 tttctgtgca aagcgcacca cttcaaactg tttcagcgct gcaatctgcc gacgaataat
    50701 ttgcgcatcg tagacccaca ccgggcagcc aaattcagcg ggcaaacgca gcagattttc
    50761 ggcggtgaga tcggtatcgg tgctgaacag tgaatgtggc ataacaaact ccagatgagt
    50821 gcttttttat gattacgcca catcataaaa agaataaaaa atatcgattt atgtcgagtc
    50881 tatgcaaaaa tgatatggat taccggattg cgagagagcg ctaatggccg ccgttaactt
    50941 acgtcatatt gaaatttttc atgcggtaat gaccgccgga agcctgactg aggcagcaca
    51001 cctgctacac acctcacagc caaccgtcag ccgcgaactg gcgcgctttg agaaggtgat
    51061 cgggctgaaa ttgtttgagc gcgtacgtgg acgattacat cctaccgtgc aaggactgcg
    51121 tctgtttgaa gaagtgcaac gatcctggta cggactggat cgcattgtca gcgccgcaga
    51181 aagtctgcgc gagtttcgcc agggagaact gtctattgcc tgcctgccgg tcttttcgca
    51241 atctttttta ccgcagctcc tgcaaccctt tctggcacgt tatcccgatg tcagcttaaa
    51301 tatcgtgccc caggaatcac cgctacttga agagtggctc tcggcccagc gtcatgattt
    51361 aggactcact gagacactcc atacgcctac gggaacagaa cgtaccgaat tactctcttt
    51421 agatgaagtg tgtgtgttac ctccgggtca tccgctggcg gtaaaaaagg tattaacgcc
    51481 ggatgatttt cagggtgaga attacatcag cctttcccgt actgacagct atcgccagtt
    51541 gctggatcag ctatttactg aacatcaggt taaacgacgc atgatcgtag aaacccacag
    51601 cgccgcgtca gtctgcgcaa tggtacgggc gggggtaggt atttcggtgg ttaacccgct
    51661 caccgcacta gattatgcgg caagcggttt agtggtgcgg cggttcagta ttgcggttcc
    51721 gttcaccgtc agcctgatcc gccccctgca ccgcccgtca tcagcgctgg ttcaggcgtt
    51781 tagtgggcat ttacaagcgg ggttaccgaa actggtcact tctcttgacg ctattttgtc
    51841 gtcagctacg acagcataaa agcgacagca tcctcggcat ggatcgccgc ggtatcaaac
    51901 acaggcaaaa cactgcgctc ttctggcacc agtaaaccaa tttctgtgca gccaaaaatg
    51961 acgccctgtg cgccctgttc tgcaaggcga gcaatcactt gcgcataata agcgcgtgac
    52021 gcttcggtaa attgccccag acacagttct tcaaaaataa tctgattaat tttcgcccgt
    52081 tcatccgctt caggaataag acaattgatg gaaaattgtt ccgtcagccg cccgcgataa
    52141 aaatcctgtt ccatggtgta acgcgtaccc agcagcgcca cacgagtcat tccggccccg
    52201 gtaattgcac gtccggtggc atccgcaatg tgtaagaaag gcagagtgca acgtgactca
    52261 atggcatccg ccactttatg catcgtattg gtacataaca caataccttc tgcgcccgcc
    52321 cgctgtaagc caagcgccgc ctcagccaga atgtccccgg ttttatccca ttccccgcga
    52381 cgctggcact cttctatttc atgaaaatcg acgctatgta gcagcatttg cgcagagtga
    52441 agcccaccaa gccgctgttt aatgccttca tttatcagac gatagtaagg aatggtggat
    52501 tcccagctca ttcctcccag caaaccaatt gttttcattc ctctctcctg atgtgtgtta
    52561 ccgcaacaag gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct
    52621 attaacgaaa aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat
    52681 ttctcaactt ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg
    52741 tttccggaat gagccagaaa gtaataccca caaacgcaat gttcagcgca gtgtagagcc
    52801 agaacgtacc ggcagcgcca atgctatcaa gcagtgtcag gaaggccgcg ccgataatca
    52861 tattcgacac ccagtttgtc gtcgtcgaac aggtaatacc gaaatcgcgg catttcagcg
    52921 gctgaatttc agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa
    52981 tacacatcat cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat
    53041 caaactgcat caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa
    53101 ttttcagagc cggtttacga cctgctttat ctaccgtaaa gaccgcaatg aaggtcgcga
    53161 acataaaggt cagtcccacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc
    53221 ccgccatttt gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct
    53281 gcatcgcctg caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa
    53341 acagtgccca accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc
    53401 gcgctttttc cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc
    53461 ccttttccgc cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagaa
    53521 ctgctggtaa agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg
    53581 tatcggataa aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca
    53641 tcttaccgcg aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg
    53701 cgatcccgac agcaatgccc agcaccacac gagcggcgat taacatctct acgctggtcg
    53761 caaaagcgga ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt
    53821 tacgccccag gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca
    53881 tcatgctact aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga
    53941 acggcaacgc tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag
    54001 cagctaccga aacaaacata ttcatacgcc gcgtatcacg caaagaacgt ggcgttaaag
    54061 cagattccgt attgatagta accatttttt cctgccagca gagagtaaga cattgtgaaa
    54121 aaatacgtga acaattcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt
    54181 gctgcgacat gccgttatgt gatggatatt ccaattttaa aattaagttg aattattgag
    54241 attattatta acctcctaat tttaaagcag ataaaattca taaagttcat taattgataa
    54301 ttgatatgga ttatttcata accatgatat ggattatgat gatctagaga tataaaaaac
    54361 cctgccatgc ggcagggtca taaaagtaag aggaatgaat taacgcgcca gccaaccgcc
    54421 atccacagca atggtataac catttacata atctgaagcg ctggaggcaa gaaacactac
    54481 cggccccatc aggtcactcg gcagtcccca acggccagct ggaatgcgat cgagaatttc
    54541 cgcgctacgt tgttcatctg cccgcagttg ttgggtattg ttggtcgcca tgtaacccgg
    54601 agctatcgca ttaacattaa tgttgtgttt agcccattcg tttgccatca atcgcgtcac
    54661 acccatcacg ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga
    54721 gagcattgac gcgatattga taatcttgcc gccattgcct tgcgcgataa agtgtttcgc
    54781 cgctgcctga gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc
    54841 tttttcgctg aactcgagag cgtcttcgcg gcgaatcaat ccggcgttat tcaccaggat
    54901 atcaatatga ccaaattcag ctaccgcgcg atccagcagt ccaggaatgc catcaatctt
    54961 tcgcagatcg gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gctcgatggt
    55021 ttcagtcggt tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa
    55081 cgccatcccc tggcccagcc cagtatcaca accagtgacg accgcaactt taccttggag
    55141 agaaaatgca cttaaaatca taacaacacc ttattcgttt atgcccacaa ctagcgcaaa
    55201 tctttaacgc ccacatggtc catatcatta aagacctggt tttcaccgac catgccccag
    55261 ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctcgg ggagatcacc
    55321 gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat catgtggaaa
    55381 acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg ctcgtgggtg
    55441 tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc catactcaat
    55501 tgacaggttt ccagtacatc cgggacaaaa tatttgttaa tcgtgcgacg gttactggtg
    55561 aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt ggtgggatac
    55621 gtcgtatgcg cgggtgcgca attgtaataa aacttcgccg gagtggcggt atcaatactg
    55681 gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg accgatttca
    55741 tagcattgac catcgacagt aatcgtaccg gcaccgccaa tattgataac acctaactcg
    55801 cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc aacggaaacc
    55861 gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct gtaaaccatg
    55921 gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag cccttgggta
    55981 tccagcgttt ttgcgtgcgc accgtggatg ctctgtctta cgtccacatc aacctccgat
    56041 aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt tctctataaa
    56101 gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt tttattttta
    56161 atttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg
    56221 agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg
    56281 gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa tggtcaatgc
    56341 cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat tacgtttcac
    56401 catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag
    56461 tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac
    56521 cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat cagccaactg
    56581 ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc
    56641 cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta aattcaacgc
    56701 tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta tggatgatgc
    56761 attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg ctaaaccttc
    56821 tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct gaccgttact
    56881 ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc
    56941 tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc catattcgcg
    57001 agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc acaacccatc
    57061 atgcaccaga ctgtcaacca actggctatt gccaagctgt tcaccggtgc ggctatcagt
    57121 cagaacatgt ggtgcgcggc tcatgttttc ctggccacct gcgatgacaa tatcagcctc
    57181 gccacactgt atcgcctgag tagccagatg cagtgcttta agaccggaac cgcaaacgtc
    57241 attaatcgtg attgcagaaa cgctgttagg aagaccacct ttaatagccg attgccttgc
    57301 cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat ccaccgcata
    57361 tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa gttccacggc
    57421 ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg ccccgacaat
    57481 cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa tgattgttaa
    57541 ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa tatgcctgag
    57601 cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac caatagatgg
    57661 gtagataggg cgttaatctc ccatacttaa cttggtttat ggtaaattgc cctccatttt
    57721 gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa gaagaaactc
    57781 tgtgaagttt cgcgctctat ggcacagccg ttgatgcagg cacgctattc cttcctattc
    57841 agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc tggcctttaa
    57901 catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca tcagcaggtg
    57961 aagggataac gggcgcggta acacactact atggcaagaa gattggtaat ctgattacca
    58021 cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca attaccaact
    58081 cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt atgttggtga
    58141 gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat gccactattc
    58201 gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt tccatttacc
    58261 ttgtcggtag ttggcaacct gatctattaa caacccaggt agagttcaat cagaataccc
    58321 ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat acgcccatta
    58381 tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg gataaatgca
    58441 aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc tttgtcttta
    58501 gctgcctgct ttctattcca tcttcgtata ttgaagcggc taaagaagaa ggggtcacca
    58561 ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt tccgggatta
    58621 ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc gttattgaag
    58681 gtgccacaga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa agtcgtgcat
    58741 ttaaccacgc actatcaatt atgttggtat cgctgattac cttcattgtt tgttgcatta
    58801 acccgaacgc gatttcgatg atttacgcga tcagcggccc gctcattgcc atgatacttt
    58861 tcatcatgcc tacgctgtca acgtatctca tcccggcgct taaaccctgg cgttccatcg
    58921 gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat
    58981 atccgttgtg gatggttcaa aattgctaca attttgaacc attccatttt tcataatatt
    59041 catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta tcagttagtt
    59101 aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat aacttagatt
    59161 cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct cataccatga
    59221 gatagttatc tggaccacgt tcgaccatgc aagattaaat cagtgaaaca taatcatatt
    59281 taataacgag aaatgcattt ttaaatatct taatcagaat tatagcaaat acagattaat
    59341 cgcaattttt cccagcaaaa ttacaaaatc aaggtgtatt gacatcatca atcaccaatg
    59401 aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt agtcgttata
    59461 aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa gagtcaccgc
    59521 gtttgcagta ctgcgca
//
LOCUS       DAJTUP010000012        58394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTUP010000012 DAJTUP010000000
VERSION     DAJTUP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.012"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1091)
                     /locus_tag="OO468_000759"
     CDS             complement(<1..1091)
                     /locus_tag="OO468_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR6257982.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQ"
     gene            complement(1303..1650)
                     /gene="pspE"
                     /locus_tag="OO468_000760"
     CDS             complement(1303..1650)
                     /gene="pspE"
                     /locus_tag="OO468_000760"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR6257983.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1693..1914)
                     /gene="pspD"
                     /locus_tag="OO468_000761"
     CDS             complement(1693..1914)
                     /gene="pspD"
                     /locus_tag="OO468_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR6257984.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1923..2282)
                     /gene="pspC"
                     /locus_tag="OO468_000762"
     CDS             complement(1923..2282)
                     /gene="pspC"
                     /locus_tag="OO468_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR6257985.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2282..2506)
                     /gene="pspB"
                     /locus_tag="OO468_000763"
     CDS             complement(2282..2506)
                     /gene="pspB"
                     /locus_tag="OO468_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR6257986.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2560..3228)
                     /gene="pspA"
                     /locus_tag="OO468_000764"
     CDS             complement(2560..3228)
                     /gene="pspA"
                     /locus_tag="OO468_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR6257987.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3395..4372
                     /gene="pspF"
                     /locus_tag="OO468_000765"
     CDS             3395..4372
                     /gene="pspF"
                     /locus_tag="OO468_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR6257988.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4492..5757)
                     /gene="puuE"
                     /locus_tag="OO468_000766"
     CDS             complement(4492..5757)
                     /gene="puuE"
                     /locus_tag="OO468_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR6257989.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5795..7075)
                     /gene="puuB"
                     /locus_tag="OO468_000767"
     CDS             complement(5795..7075)
                     /gene="puuB"
                     /locus_tag="OO468_000767"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR6257990.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7077..8564)
                     /gene="puuC"
                     /locus_tag="OO468_000768"
     CDS             complement(7077..8564)
                     /gene="puuC"
                     /locus_tag="OO468_000768"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR6257991.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8714..9271)
                     /gene="puuR"
                     /locus_tag="OO468_000769"
     CDS             complement(8714..9271)
                     /gene="puuR"
                     /locus_tag="OO468_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR6257992.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9298..10062)
                     /gene="puuD"
                     /locus_tag="OO468_000770"
     CDS             complement(9298..10062)
                     /gene="puuD"
                     /locus_tag="OO468_000770"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR6257993.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10274..11692
                     /gene="puuA"
                     /locus_tag="OO468_000771"
     CDS             10274..11692
                     /gene="puuA"
                     /locus_tag="OO468_000771"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR6257994.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11841..12005
                     /locus_tag="OO468_000772"
     CDS             11841..12005
                     /locus_tag="OO468_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6257995.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11995..13380
                     /gene="puuP"
                     /locus_tag="OO468_000773"
     CDS             11995..13380
                     /gene="puuP"
                     /locus_tag="OO468_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR6257996.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13514..13759
                     /locus_tag="OO468_000774"
     CDS             13514..13759
                     /locus_tag="OO468_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR6257997.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14072..15715
                     /gene="sapA"
                     /locus_tag="OO468_000775"
     CDS             14072..15715
                     /gene="sapA"
                     /locus_tag="OO468_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR6257998.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15712..16677
                     /gene="sapB"
                     /locus_tag="OO468_000776"
     CDS             15712..16677
                     /gene="sapB"
                     /locus_tag="OO468_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR6257999.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16664..17554
                     /gene="sapC"
                     /locus_tag="OO468_000777"
     CDS             16664..17554
                     /gene="sapC"
                     /locus_tag="OO468_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR6258000.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17554..18546
                     /gene="sapD"
                     /locus_tag="OO468_000778"
     CDS             17554..18546
                     /gene="sapD"
                     /locus_tag="OO468_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR6258001.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18548..19354
                     /gene="sapF"
                     /locus_tag="OO468_000779"
     CDS             18548..19354
                     /gene="sapF"
                     /locus_tag="OO468_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR6258002.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19422..19775
                     /locus_tag="OO468_000780"
     CDS             19422..19775
                     /locus_tag="OO468_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR6258003.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20143..20931
                     /gene="fabI"
                     /locus_tag="OO468_000781"
     CDS             20143..20931
                     /gene="fabI"
                     /locus_tag="OO468_000781"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR6258004.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21075..22202
                     /locus_tag="OO468_000782"
     CDS             21075..22202
                     /locus_tag="OO468_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR6258005.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22270..24204
                     /gene="rnb"
                     /locus_tag="OO468_000783"
     CDS             22270..24204
                     /gene="rnb"
                     /locus_tag="OO468_000783"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR6258006.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24282..24385)
                     /locus_tag="OO468_000784"
                     /pseudo
     CDS             complement(24282..24385)
                     /locus_tag="OO468_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24439..26413
                     /gene="pdeR"
                     /locus_tag="OO468_000785"
                     /pseudo
     CDS             24439..26413
                     /gene="pdeR"
                     /locus_tag="OO468_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26561..26734
                     /locus_tag="OO468_000786"
     CDS             26561..26734
                     /locus_tag="OO468_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258007.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26824..27573
                     /locus_tag="OO468_000787"
     CDS             26824..27573
                     /locus_tag="OO468_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6258008.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27842..28060
                     /gene="osmB"
                     /locus_tag="OO468_000788"
     CDS             27842..28060
                     /gene="osmB"
                     /locus_tag="OO468_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR6258009.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28186..28512)
                     /gene="yciH"
                     /locus_tag="OO468_000789"
     CDS             complement(28186..28512)
                     /gene="yciH"
                     /locus_tag="OO468_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR6258010.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28512..29249)
                     /gene="pyrF"
                     /locus_tag="OO468_000790"
     CDS             complement(28512..29249)
                     /gene="pyrF"
                     /locus_tag="OO468_000790"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR6258011.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29442..30611)
                     /gene="lapB"
                     /locus_tag="OO468_000791"
     CDS             complement(29442..30611)
                     /gene="lapB"
                     /locus_tag="OO468_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR6258012.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30618..30926)
                     /gene="lapA"
                     /locus_tag="OO468_000792"
     CDS             complement(30618..30926)
                     /gene="lapA"
                     /locus_tag="OO468_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR6258013.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31075..31839)
                     /gene="pgpB"
                     /locus_tag="OO468_000793"
     CDS             complement(31075..31839)
                     /gene="pgpB"
                     /locus_tag="OO468_000793"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR6258014.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32009..32599
                     /gene="ribA"
                     /locus_tag="OO468_000794"
     CDS             32009..32599
                     /gene="ribA"
                     /locus_tag="OO468_000794"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR6258015.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32663..35338)
                     /gene="acnA"
                     /locus_tag="OO468_000795"
     CDS             complement(32663..35338)
                     /gene="acnA"
                     /locus_tag="OO468_000795"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR6258016.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35502..35597)
                     /locus_tag="OO468_000796"
     CDS             complement(35502..35597)
                     /locus_tag="OO468_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258017.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35711..35878)
                     /locus_tag="OO468_000797"
     CDS             complement(35711..35878)
                     /locus_tag="OO468_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258018.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35881..36045)
                     /locus_tag="OO468_000798"
     CDS             complement(35881..36045)
                     /locus_tag="OO468_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR6258019.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36340..37314)
                     /gene="cysB"
                     /locus_tag="OO468_000799"
     CDS             complement(36340..37314)
                     /gene="cysB"
                     /locus_tag="OO468_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR6258020.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37524..40121)
                     /gene="topA"
                     /locus_tag="OO468_000800"
     CDS             complement(37524..40121)
                     /gene="topA"
                     /locus_tag="OO468_000800"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR6258021.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40501..40752
                     /locus_tag="OO468_000801"
     CDS             40501..40752
                     /locus_tag="OO468_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR6258022.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40788..41837)
                     /gene="sohB"
                     /locus_tag="OO468_000802"
     CDS             complement(40788..41837)
                     /gene="sohB"
                     /locus_tag="OO468_000802"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR6258023.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42057..42815
                     /locus_tag="OO468_000803"
     CDS             42057..42815
                     /locus_tag="OO468_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR6258024.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42812..43402
                     /gene="cobO"
                     /locus_tag="OO468_000804"
     CDS             42812..43402
                     /gene="cobO"
                     /locus_tag="OO468_000804"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR6258025.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43442..44314)
                     /gene="rluB"
                     /locus_tag="OO468_000805"
     CDS             complement(43442..44314)
                     /gene="rluB"
                     /locus_tag="OO468_000805"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR6258026.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44415..45035)
                     /locus_tag="OO468_000806"
     CDS             complement(44415..45035)
                     /locus_tag="OO468_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR6258027.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45032..45913)
                     /gene="yciV"
                     /locus_tag="OO468_000807"
     CDS             complement(45032..45913)
                     /gene="yciV"
                     /locus_tag="OO468_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR6258028.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46051..46095
                     /gene="trpL"
                     /locus_tag="OO468_000808"
     CDS             46051..46095
                     /gene="trpL"
                     /locus_tag="OO468_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR6258029.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46072..46165
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46187..47749
                     /gene="trpE"
                     /locus_tag="OO468_000809"
     CDS             46187..47749
                     /gene="trpE"
                     /locus_tag="OO468_000809"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR6258030.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47749..49344
                     /gene="trpD"
                     /locus_tag="OO468_000810"
     CDS             47749..49344
                     /gene="trpD"
                     /locus_tag="OO468_000810"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR6258031.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49345..50706
                     /gene="trpCF"
                     /locus_tag="OO468_000811"
     CDS             49345..50706
                     /gene="trpCF"
                     /locus_tag="OO468_000811"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR6258032.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50718..51911
                     /gene="trpB"
                     /locus_tag="OO468_000812"
     CDS             50718..51911
                     /gene="trpB"
                     /locus_tag="OO468_000812"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR6258033.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51911..52717
                     /gene="trpA"
                     /locus_tag="OO468_000813"
     CDS             51911..52717
                     /gene="trpA"
                     /locus_tag="OO468_000813"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR6258034.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53097..53276
                     /locus_tag="OO468_000814"
     CDS             53097..53276
                     /locus_tag="OO468_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR6258035.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53362..53862
                     /locus_tag="OO468_000815"
     CDS             53362..53862
                     /locus_tag="OO468_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6258036.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53908..54414
                     /locus_tag="OO468_000816"
     CDS             53908..54414
                     /locus_tag="OO468_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR6258037.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54474..55112)
                     /gene="ompW"
                     /locus_tag="OO468_000817"
     CDS             complement(54474..55112)
                     /gene="ompW"
                     /locus_tag="OO468_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR6258038.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIAIVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55352..55441
                     /locus_tag="OO468_000818"
                     /pseudo
     CDS             55352..55441
                     /locus_tag="OO468_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55469..56212
                     /locus_tag="OO468_000819"
     CDS             55469..56212
                     /locus_tag="OO468_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR6258039.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56242..56781
                     /locus_tag="OO468_000820"
     CDS             56242..56781
                     /locus_tag="OO468_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR6258040.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56886..57284
                     /gene="yciA"
                     /locus_tag="OO468_000821"
     CDS             56886..57284
                     /gene="yciA"
                     /locus_tag="OO468_000821"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR6258041.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57324..58052)
                     /gene="tonB"
                     /locus_tag="OO468_000822"
     CDS             complement(57324..58052)
                     /gene="tonB"
                     /locus_tag="OO468_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR6258042.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58267..>58394
                     /locus_tag="OO468_000823"
     CDS             58267..>58394
                     /locus_tag="OO468_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258043.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGP"
BASE COUNT        14018 a        15103 c        14589 g        14684 t
ORIGIN      
        1 tcctgctgta atgcctcgac cagcgctaat atttcgcttt ctggaagcaa gccacgcagt
       61 gggttcaacc cgatgccatc gtgagaggcg aggaagttaa accaggtcgt tttactggag
      121 ggcaagctca gactttgcgc ccactggcac agcgcctcga cgttctgttt ttgcaccgca
      181 tgtaacacca gcggcggcag cgagaactgg tacaccatat gcgcttcgtc atcaccgttg
      241 ccaaagtaag caatgttgtc tttgtgcgga acattggttt cggtaataat cactgtacct
      301 ggtgctacgt catcaataat cgaccgtaac agcttgataa tcagatgtgt tttttccaga
      361 tggatgcagc ttgttcccgg ctctttccac ataaagccaa cggcatccag gcggacatac
      421 tcagcacctt tctcaaggta acagagtaaa acatccacca tcgccagcaa cacttcaggg
      481 ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg tccataaatg gcgcgtggaa
      541 ttatcatcca tttgaaacgg cgttaatagc ggtaacgcac gcggaagggt gacggcactg
      601 agatcggttt tcggatcaac cgcaataaag aaatcttcaa aacccggaag ctgttgtaaa
      661 tagtttttaa accattcact ttttgcagac atatggttgc agacaaaatc aaacattaaa
      721 tgactgcatt caccgagttg ctgaatatcc tgccactccc ccgcttcact ggcgacctga
      781 tgataatcaa ttaccgaaaa tccatcatca gatgaccatg ggtaaaaagg taataaatga
      841 acatgtgaaa aaatgctttg cagccattga tggtagaact gattaaatat gggtagtggt
      901 tttaaatcat tactgtgaaa ttgatcggca taggtaatta acacgacatc actttcatcc
      961 cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac tctcaagacg cgttacaagt
     1021 ttctgtaaat gagttgcggt aaatgttcca ccgtagattt cgtccatgta atccgtaatt
     1081 ttctgtttca tgttgccttt ccgtggtagc ggtcccatga ggcgagagac tactccgcag
     1141 aaaataaact gtcaattgag cacaagggtt ttagtcacgc aggaaatcat tttttaccag
     1201 ggaaaaagcg tatttgtgag ctgacgcaaa taatctatag ggatgtaaat aaggtgtgac
     1261 agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc ttttaacctt tgaccttcgg
     1321 cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg gtatatccca tctcgctaag
     1381 gagctctttt gcctgcccgg actggcgtcc ggcattgcaa tacactttca cggtgtcgtt
     1441 tttatccgga acggcggtgg caatgcgctc tttcacttcc ttcaggggaa tattgatggc
     1501 cccctgaacg tgctcttgct gatactgctc tggaacacga acatcgatcc agtgttcagc
     1561 ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt aagccttttt ttaaacatgt
     1621 aagcgtcctt ctatggatga aaattgccat ccattatacg cctgagattt aacaattttt
     1681 gcgaaactcc cctcaccttt tataacgctg tgccagttta ttagcagccc gacttaacag
     1741 cggttccagc gccacagcca gcaacatttt cagcggtcgg cgagcaactg attttatcgc
     1801 ccagcccgcc acacccgccg ggccatagcg cagtgcggta agaagtacca gcttgcctgc
     1861 taatttgaaa ccaagcttca ccttttgccc ggcctgttgc cagcgagtat tcataacttt
     1921 cctcacagtt gacggaaacg gctacgtaac gtgaaagtat cggaagtgac ataacgttcc
     1981 atctcgcgta aacgcgtttc acttgccgcc agttcacggt cgacttcatc cagcaattcg
     2041 ctgctggaag gtagctgctc accaaaggcc atgttgtccg gcattggatc aagcgcaaat
     2101 gacaaaatga tgtaagcaac cagggtaaac agcgccagac cgaaaaaaat cgacagcacc
     2161 accaggatac gcaccagttt taccggtaca tcaaaatagt tggcaatccc ggcgcagacg
     2221 ccgcggacca tgccctgctg tggaatacgc cataactttt tattgagatt aatgcccgcc
     2281 attagcgatc cctccagttc ggatgttctg catcaagaat agattccagc gcctgaatac
     2341 gttcgcgcat ccgttttgct tcatcaacca gttgcgctaa tcgctgctgc tcactttgcg
     2401 acaattcact gcgaccagaa cgattgctgt aatgcagcca taaccagatc ggtaaaacaa
     2461 acagcacaaa aatggttaac ggaatagcca gaaatagcgc gctcataagt actccttaga
     2521 cgatgagcgg cggcgcgttc ggacgccgcc tggatgttat tattgattgt cttgcttcat
     2581 tttggctttt aattgtgcca gttgttcgct gattgcatca tcggctttca gttcggcaaa
     2641 ctgatcgtcc agcgattttt gtttaccgaa gctgtggctt tctgcttccg cttccatctg
     2701 gtcaatacga cgttcgaaag attcgaaacg agccattgct tcatccagtt tgccactgtc
     2761 cagctgacga cgcacatcgc gcgacgagtt tgccgcctga tgacgtaaca tcaatgcctg
     2821 ctggcgagcg cgtgtttcgc tcaatttgtt ttccagctcg ccaatctctt tcttcatgcg
     2881 tgccagcgta tcgtccacca gcgtcacttc atgttccagg gacttaatca gatcggtcag
     2941 tttctgtttt tcaattaacg ctgcacgtgc cagatcctct ctctctttca gcagcgccag
     3001 ttcggctttt tcctgccatt caacttcacg cgccgacgct tgttcaatac ggcgagtcag
     3061 ctgtttcttt tctgccaacg cacgcgccga agtagaacgt acttcaacca gtgtatcttc
     3121 catctcctgg atcatcagac gcaccagttt ctgtggatct tccgctttct ctaacagagc
     3181 gttgatgttg gcattcacga tgtcggcaaa gcgagaaaaa atacccataa tgttgtcctc
     3241 ttgatttctg cgttcaggat tgtcctgctg aactgttaat acaatttgcg tgccaatttt
     3301 ttatcttttt gatttataaa gatctgattg aagaatcaac agcaacatgc caggatgaat
     3361 tagcgaatta cactaacaag tggcgaattt catcatggcc gaatacaaag ataatttact
     3421 tggtgaggcg aacagctttc tcgaagtgct ggaacaggtt tcgcatctcg caccgctgga
     3481 caaaccggtg ctcatcatcg gcgaacgcgg caccggtaaa gagttgattg ccagccgcct
     3541 gcattatctc tcctcccgtt ggcaagggcc gtttatttcc cttaactgcg cggcgttaaa
     3601 tgaaaatctg ctggattccg aactgtttgg tcacgaagcg ggggcgttta ccggtgcgca
     3661 aaaacgtcat cctgggagat ttgaacgtgc cgacggcggt acgctatttc ttgatgaact
     3721 cgctacggca ccgatgatgg tgcaggaaaa attattgcgt gtgattgagt acggtgaact
     3781 ggagcgcgtt ggcggtagcc agccgttgca ggtgaatgtg cggttggtat gcgcgacgaa
     3841 tgccgatctc ccggcgatgg tcaatgaagg tacttttcgc gctgacctgc tcgaccggct
     3901 ggctttcgat gtagtgcaac tgccgcccct gcgcgagcgc gaaagcgaca ttatgctgat
     3961 ggcagaacac tttgccattc agatgtgtcg ggaaatcaag ctgcctctgt tcccggggtt
     4021 tacggagcgc gccagagaaa cattgctgaa ttatcgttgg ccgggaaata ttcgtgaatt
     4081 gaaaaacgtg gtggaacgtt cagtgtatcg ccacggcacc agcgattatc cgcttgatga
     4141 catcattatt gatcccttta aacggcgtcc acctgaagaa gccatcgccg tttcagaaaa
     4201 tacctcgctt ccgacgctgc cgctggattt acgcgagttt cagatgcagc aggaaaaaga
     4261 gttgctgcaa ctcagtttgc aacaggggaa atataaccag aagcgcgcgg ctgaattact
     4321 ggggttaact tatcatcagt tccgcgcgtt gttgaaaaag caccagattt agcgaagttg
     4381 agcaatacaa cttgccggat gcggcgtgaa tgctttattc ggcatacata atcaatacgt
     4441 cgtttgcagg cctgataagc gcagcgcatc agacattatt gcgttgggct attaatcgct
     4501 cagcgcatcc tgcaaaattt tcattgccgc atcgaattgc gcatccggga tggtcagcgg
     4561 atacaggaag cgaatcacgt tgccgtatgc gccacaggtc agcaggagca gcccctgcgc
     4621 cagcgcgcgt tgctggattt tctgtgcaat cgccgctgac ggctcgcccg tttgcggatc
     4681 gttaaactct gccgcaatca tcgaccccag gccgcgtacc gcagcaatgg ccggaacgct
     4741 ttctttggca tcaatcaacg tgtttttgag acgctggccc agttgattcg cgcgttcgca
     4801 gagtgattct ttgtcgataa tattgagcac cgcgtgcgcg gcagccaccg ccagcgggtt
     4861 cccggcgtag gtgccaccca acccgcccgg cgcgggcgcg tccataatat tcgcgttacc
     4921 gaccacgccc gaaagcggca tcccgcccgc gaggcttttc gccatcgtca ttaagtccgg
     4981 cttatcggcg taatgatcca tggcaaacag tttaccggta cgcgcaaagc cgctttgcac
     5041 ttcatcggca atcatcacaa taccgtgctc gtcgcacagg cggcgaatgg cggcaaccag
     5101 ctcttttggc gcaacgttga aaccgcactc gccctgcacc ggttcgaaaa taatggccgc
     5161 gacctgcttc gcttcgatgt ctgatttaaa caagcgttcg atggcgtcga gggagtcctg
     5221 tgttgaaatg ccgtgtaaat ctgacggata aggtacgtga tacaccgaac cagggaacgg
     5281 gccgaagccg attttgtacg gcgcaacttt tccggtcagc gccatggtca tatatgtacg
     5341 accgtggaag ccaccgctaa acgcaatcac gccaggtcgt ccggtatggg cgcgagcaat
     5401 tttcaccgcg ttttccaccg cttccgcgcc ggtagtgaag aatgcggttt tagcctgccc
     5461 gctcactggg gcaagggtgt taattttctc cgccagcgtg acgtagcttt cgtacggcac
     5521 aatctgatac gccgtgtggg taaactgttg caattgctgc tccaccgccg cgaccagatc
     5581 agggtggcga tgcccggtat tcagcaccgc aatgcctgcg gcgaaatcga tgtattcgtt
     5641 gccctctacg tccttcagcg tagcgttttc tgccgactgg gcgaagaagt tacacatcac
     5701 gccaaccccg cgtggggtag cagaaagacg acgctgatgg aattcattgt tgctcatgtt
     5761 tcacaccttt tgactcttca ctgttaatcg gaaattaaac gcccagacga tcgcgcaggc
     5821 tgtaataagc cgcgcccatc gcggtaaatg gcacgcgcag cgtgcgcccg ccggggaacg
     5881 ggtaatgcgg cagattggcg aaggcatcga aacgctcggc gtcaccgcgc agcagttcag
     5941 caatcaaacg cccggcaaga tgggtacagg tgacgccgtg gccgctgtaa ccctgcatgt
     6001 aatagatgtt ggtatcaagg cggccgaact gcggcatgtg cgacaaggtc agcaggaagt
     6061 tgcccgtcca gcggtaatca attttcacgc ccttcaactg agggaaggtt tttagtagtt
     6121 tcggtaccac aagacgctca acgtcatccg ggttgcgtgc accatacact acacccccgc
     6181 cgtacagcag gcggttgtcg gcggtaagac ggtagtaatc aagcagatag ttacagtctt
     6241 ccacacagta gtttttcggg atcagcgaac gggctaaatc ttccgacagc cgttcggtgg
     6301 tgatcacctg cgtgccgcac ggcatgctgc gtttcgctag ttccggctct actttatcgc
     6361 ccagatacgc attcccggcg acaatcacat acttcgccgt cacctgacca ttggcagtcc
     6421 gaaccacggc tggcgtggtg tgctggatct gcgtcacggc ggaaagctca tacacgcgcc
     6481 cgccgttcag gcggatggca tcggcttcac caatggcgag gtttagcggg tgaatatgcc
     6541 caccgctgtg atccagcagc gcaccagtgt agcgatcgct ggcgacctcg cggcgaatag
     6601 cgttgacgtc cagcagctcc agctgtttat tgccgtagcg tacccagttc tctttctgct
     6661 cttcgagtgt ggcgagttgt ttatcattca tcgccacaaa cagaccgccg gggcggtagt
     6721 cgcagtcaat ctgataacgt ttgatacgtt cgcggatgat ctcgccaccc tcgaacatca
     6781 tgctgccgag catacgggcg gtgtccatgc cgtagctttt ttcgatcacg tcgatgtcgc
     6841 ggctgtagga gttcacaagc tgcccgccat tgcgcccgct tgcgccaaag ccgatgcgtg
     6901 aggcttcgag aaccactacg tcaaagcccg cttccgccag atgcagcgcg gaggagagcc
     6961 cggtatagcc gccgccaacc acgcaaacgt cgcaggtgat tgactcattc agtgtgtcga
     7021 atggtgcata tttattcgca ctggcggcgt agtaactgct ggtatgttcg gtcatttcag
     7081 gcctccaggc ttatccagat ggttttcagt tcagtgaatt tttcaagggc atgcagggat
     7141 ttgtcgcgac cgttgccgct ctgcttatag ccgccaaacg gcacggtcat atcgccgtcg
     7201 ttgtagttat tgacgaagac ggaaccggct ttcaggcgtc ggctcatgcg gtgcgcgcgg
     7261 gagaggtcgc gcgtccatac cgccgcgcca aggccgtact ggctgtcgtt ggcaagctgc
     7321 agcgcctgat cttctgatgt gaaacgggtg accaccagca ccggaccgaa aatctcttcg
     7381 cggcttaagg acgcattcgg gtccacatcc acgaagatgg tcgggccgat ggccgctgcc
     7441 agcccggcgt tgcgaccatc cagcagcagt tgccccttgc cttcgccttc acgaataaag
     7501 gtgtgtaccg agtcagcgtg agcgctgtcg attaaggtgc ccatggtggt tgcgggatca
     7561 agtggatggc ccggctgcca gttttgcgcc tgctgtttta acagggctaa gaattcatcg
     7621 gcgatgctct cttccagtaa caaacgcgtt ccggcgatgc gcacctggcc ctggttgtag
     7681 aagatgcctg cggcggtggc gcttgccgct ttttgcagat ccggacaatc ggcgaaaacg
     7741 atgttggcgc ttttgccgcc cgcttccagc cagacacgtt tcatgttgct atcacccgcg
     7801 tctttcagca gttgtttacc cgtacgcgtg gagccggtaa aggcgatggc atcgatatcg
     7861 ttatgacgcg aaagcgcctg ccccgcttca tgaccaaaac ccgtcaccac gttcaacaca
     7921 ccatccggca agcctgcttc tttcgccagc cccgcgagac gaatcgcact gagcggtgat
     7981 ttttcagacg gttttagaat cacgctgttt cccgccgcca gcgccgggcc gagtttccag
     8041 caagtcagca acagcgggaa gttccacggc acgatggcgg caatcacgcc gaccggttca
     8101 cgcacgatca tcgccagctc atggctactg gtggtcgcca cttcgccata cactttgtcg
     8161 atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg gaatatcatc acgcagactg
     8221 tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg ccagctcttc ggcgtgggct
     8281 tccattaaat cggcgagttt attcagtacc gctttacgtt tagccggaga agagagtgac
     8341 cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg cacggtcgat atcgacgctc
     8401 ttgccgcggg caattttcgc cagcggtgcc tgggtgaccg gatcaacggt ttcaaaggtt
     8461 tcattttccg ccgcagcagt atattcaccg ttaataaata agcggttttc aatggcgaga
     8521 cttaacgctt tatcctgcca gtaagccaga tgatgaaaat tcattatgac tcctgtttca
     8581 cgtctatcag atatatgcag tttgtgaacg tcagggcgca gagatccccg ggctgcatgc
     8641 ccgtttattt ctggcgacat tctttatgga ttgccgggcg cattgcgctt acccggctta
     8701 cagaacaaaa agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg
     8761 ccgacgtatt actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga
     8821 cgaggtggta atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta
     8881 tttcctcacc ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa
     8941 agatcatcgc cagcgtgcga ttcgggttac cgttatgaac cagcttcatt gacacaccct
     9001 gactgcccat ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt
     9061 ccggctcgga aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg
     9121 tactgatggc agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc
     9181 cggagagttc ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc
     9241 gttttcctgg cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca
     9301 gagtcgctgt ttttcagcga tatggtgctg ccaagcggtg ataaagccct cgaacaatat
     9361 acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa
     9421 aggatgattg atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg
     9481 caaccgtggg ctaacgacct tcgccccttg tccatgtaga gagtttaccc aaaagttgct
     9541 acattcaggt aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg
     9601 tgcatattgc tgttccaccg gcagttcggg atcttcccga tgttccagca attcaggctg
     9661 ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc accagttctt gtaaaccccg
     9721 gcagatggcg aaaatgggga tgcgcctttc gagtgcggca ttaattatcg ccatgctcag
     9781 aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg
     9841 cacattgctg ggactactag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag
     9901 taacgacggt tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat
     9961 ggcattcagg tacttttctt gcagagtctg ggtcgcatga cccttaagcc tgttcctgca
    10021 cattacgaca ccgataaccg gattgttcat tatattttcc atgctcaatc tcacaaaatg
    10081 gactaaatta tcgccattac tgcgctttaa atgaccttta tgttcaatat tttttcaatc
    10141 tagcagtgga ttttcatttt tgcaaactca atttaacatt tgacaaacat ttggtttgca
    10201 tacagattcg aatgatggtc attatatttt acgctttgat aacgagcgga aaacaaacca
    10261 aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga
    10321 agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa
    10381 tacgcaatat gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat
    10441 ccctgtttca agcctgaaga agctcgagaa agggtgttat ttcccggcct cggtatttgc
    10501 aatggatatt ctgggcaacg tagtagaaga agcaggtctg ggtcaggaaa tgggcgagcc
    10561 ggatcgtacc tgtgttcctg ttctcggttc cttaactcct tctgccgccg atccagagtt
    10621 tatcggtcag atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc
    10681 gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag cgcggattgt tcccggttgt
    10741 agcggtagag ctggagttct atttactgga tcgccagcgc gacgctgaag gggatctgca
    10801 accgccctgc gcgcctggca ccgatgaccg caatacacaa agccaggttt actccgttga
    10861 taacctcaac cacttcgccg acgtgctcaa tgatattgat gaactggcgc agttgcagct
    10921 gattccggca gatggcgcgg tcgctgaggc ctcgccgggt cagtttgaaa tcaaccttta
    10981 ccatactgat aacgtgctgg aagcctgcga tgatgcgctg gcactaaaac gcctagtgcg
    11041 tctgatggca gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca
    11101 cgcgggcagc gggatgcata tccatatcag tatgcaaaat aatcgtggcg agaatgtgct
    11161 ttctgacaca gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga
    11221 cctgatgccg tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca
    11281 gccgggaatg tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct
    11341 gcgtattccg tgcggtgacc gtcataatca ccgcgtggaa tatcacgtgg cgggtgccga
    11401 tgccaaccca tatctggtga tggcagcgat ttttgccggt attttgcatg gccttgataa
    11461 cgagctgccg ttgcaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt
    11521 cccgattcgc cagagcgatg ccctgggtga gtttatcgag aatgatcacc tgcgccgcta
    11581 tttaggcgaa cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt
    11641 tgagcgcctc atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aattcccctt
    11701 gcggggttgt ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca
    11761 tttcccggat gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac
    11821 tgtgacgagg aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg
    11881 tatgcgcccc tcctgctact gactggactc cgcccccttt gcttcccttg ctaaacaggt
    11941 ttgatttcag gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct
    12001 attaattcac cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg
    12061 aaattgtggc aggtggtaat gatgggtctg gcctatctca cgccgatgac cgtatttgat
    12121 acttttggta ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg
    12181 ctggcgggcg tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag
    12241 gccggttcgg cctataccta cgcgcaaaag tcgattaacc cgcacgtcgg atttatggtc
    12301 ggctggtcat cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa
    12361 atctatctct ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc
    12421 gccattttaa ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg
    12481 tttgtactgg tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg
    12541 cataaaggag aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg
    12601 cacctgatcc cgattattac cggtgcgacg attgtctgtt tctcgttcct cggtttcgat
    12661 gcggtgacca cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc
    12721 ttcctgacgg cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg
    12781 ttctttcccg atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg
    12841 tacgtcggcg gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt gaacacatta
    12901 gcgtctggcc tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac
    12961 aatgtgtttc cggagcgcgt gtttggctat gtgcacccaa aatggcggac tccggcactc
    13021 aacgtcatta tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg
    13081 acagcactga ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt
    13141 aatcatttct ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg
    13201 ctgatgccgc tggttggcgc gctgacggtg ggtgtgctgt gggttaacct agagtcaacg
    13261 tcactgacac tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc
    13321 cgccgctatc gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa
    13381 tagccggatg cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca ttaactataa
    13441 aaagatgtat gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc
    13501 ccaggggcaa ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta
    13561 cgcgaccgaa tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg cccactactt
    13621 ccagttactg ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga
    13681 aatggctgct gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa
    13741 tcaatggggt aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgtccggat
    13801 aaagcgttca cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt
    13861 tctcacgatc gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga
    13921 aatcacgggt acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg
    13981 gccctcatta cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag
    14041 atagcctctt tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt
    14101 gattgctgga cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga
    14161 tatccgcgac agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc
    14221 caaagcgagc agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct
    14281 ggatgtcgat ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact
    14341 cgacaacggc gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga
    14401 ctggtttact cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat
    14461 ttttgaccgc aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag
    14521 cctgcaattt gccgataacg tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt
    14581 ccgactcgcc cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt
    14641 catgtcggca gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg
    14701 tcaaccggtc ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatttattcg
    14761 cctacaacgt catgatgact tctggcgcgg taaaccgtta atgccgcagg tagtggtgga
    14821 tttaggctcc ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct
    14881 ggcctggcct gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac
    14941 gctgcgtcct gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa
    15001 taatcccgct gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc
    15061 catctattat ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta
    15121 tgacaacgag gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc
    15181 gttggggctg gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa
    15241 ccccagtcca ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa
    15301 agtggtgatt gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca
    15361 tgatctgacg ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc
    15421 gttactgagc tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcaatccgaa
    15481 attcgacagc gtgttgcgta aggcgctctc ctcgcagcag ctggcagcgc gtattgaagc
    15541 ctatgacgaa gcgcagagta ttctggcgca ggaattacct attctgccgc tggcgtcgtc
    15601 attgcgtttg caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa
    15661 cgcctccttt gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc
    15721 ttcaccttac gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc
    15781 ttcagcttga gctatttcac tccacacgcg ccgttacaag gcgcgtcact gtggaatgcc
    15841 tgggtgttct ggtttaatgg cctgatccac tgggattttg gtgtttccag cattaacggt
    15901 caaccaattg ccgaacagtt aaaagagctc ttcccggcga cgatagaact gtgcatcctc
    15961 gccttcggtt ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc
    16021 cataagtggc aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg
    16081 ttctggctgg cgcttctgtt gacactgttt tgttcactca cgctaggttg gttgcccgtt
    16141 tcagggcgtt tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat
    16201 gcctggcttt cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg
    16261 atattgcccg tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt
    16321 atcagcacta ttgaagtgta tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg
    16381 tcgcgcttta ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct
    16441 cgcctgggct tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt
    16501 agctggccgg gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc
    16561 atttccgccg gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat
    16621 attttgggtg ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt
    16681 atacagcgaa aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga
    16741 cgcctctgcg atggtcggcc tgtacggctg cgcgggactg gctgtactgt gtatttttgg
    16801 cggctggttt gcgccttacg gcatcgacca gcaatttctc ggttatcaat tattgccgcc
    16861 atcatggtcg cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgtga
    16921 tgttttaagc cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac
    16981 gcttgccgcg acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct
    17041 ccgctcagcg gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgtt
    17101 ggcaattatc gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg
    17161 gctggcattg ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga
    17221 aaaagagtac gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt
    17281 tgctgtgatg ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat
    17341 ggcaattctc gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc
    17401 tgaatgggga gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt
    17461 catgctgcca ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc ttggcgatgg
    17521 cgttcgtcgt gcgattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg
    17581 accattgaat ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc
    17641 ttaaccgaag gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt
    17701 gcgaaagcga tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt
    17761 tttgatgata tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat
    17821 aacgtgtcga tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt
    17881 cgccagttga tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt
    17941 ggctggcgca aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa
    18001 gatgcgatgc gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata
    18061 gccattgcac tggcgaatca accgcgtctg ctgattgctg acgaaccgac aaactcaatg
    18121 gagccaacaa cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagcaat
    18181 accactattt tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt
    18241 aacgtgcttt actgcggtca aacagtggaa accgcgccga gtaaggagtt ggtgacgatg
    18301 ccacatcatc cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg
    18361 ccgcataaaa gtcgcctcaa tactctaccc ggcgctatcc cattgctgga acagttaccg
    18421 attggttgtc gtctggggcc acgttgcccg tatgcacaac gagaatgcat tgtgacgcca
    18481 cgtttgacgg gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa
    18541 gagtgagatg atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg
    18601 gaccggttgg tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg
    18661 tgaaggccag acactggcga ttattggcga gaatggttcg ggtaaatcca cactggcaaa
    18721 aatgctggcg ggaatgatag aacctaccag cggcgagtta ttgattgacg atcatccgct
    18781 gcattttggc gattattcct tccgtagtca gcgtattcgc atgatttttc aggatccttc
    18841 gacctcgttg aatccccgtc aacgtatctc acaaattctg gattttccac tgcgcctgaa
    18901 caccgacctg gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg
    18961 gctgctaccg gatcacgtca gttactatcc gcatatgttg gcacccggac aaaaacagcg
    19021 tctgggtctg gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct
    19081 cgcttcactg gatatgtcaa tgcgttcgca gttgattaat ctgatgctgg agttacagga
    19141 aaaacagggc atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag
    19201 cgaccaggtg ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt
    19261 gctggcatcg ccgctgcatg aactcaccaa acggctgatt gccgggcact ttggcgaagc
    19321 attgacagcg gatgcgtggc gtaaagatcg ctaattttct gaatcctcct tccagattat
    19381 tcacctcatt ctgacaagag attaaatctt gtcagaatga ggtgatggat aaaataaaat
    19441 caaatgcgcg tgatttaaga cgcaatctca ctttgcagga acgaaagctc tggcgatatc
    19501 ttcgcagccg acgttttggt gatttcaaat ttcgccgtca acatccggtg gggagctaca
    19561 ttctcgattt tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt gggcagcatg
    19621 atttggcagt tgcctatgat accaggcgca ctagctggct tgagtcgcag ggctggaccg
    19681 tgctgcgttt ctggaataac gagattgatt gtaatgagga ggcggtgctg gagattattc
    19741 tgcaggaact gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc gaccgagccg
    19801 aatagctgtt gtggtgaaaa catggagaca gtgctggaga atattcggca aggtctgaac
    19861 catcccagcc atcgccataa aagggttagg ggctgtatga gcctgtttgt tgctggggta
    19921 acaatatttg cacaatatgg tcccctcgcc cctctgggga gagggttagg gtaaggggaa
    19981 aagcgccccc cctgccgcag cctgctccgg tcggacctgg taactatagc tactcacagc
    20041 caggttgatt ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct
    20101 gattttcagg cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt
    20161 aagcgcattc tggtaaccgg tgttgccagc aaactgtcca tcgcctacgg tatcgctcag
    20221 gcgatgcacc gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc
    20281 cgcgtagaag aatttgccgc tcaattgggt tctgacatcg ttctgcagtg cgatgttgca
    20341 gaagatacca gcatcgacac catgttcgct gaactgggga aagtttggcc gaaatttgac
    20401 ggtttcgtac actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac
    20461 gccgttaccc gtgaaggctt caaaattgcc cacgacatca gctcctacag cttcgttgca
    20521 atggcaaaag cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac
    20581 cttggcgctg agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg
    20641 gaagcgaacg tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc
    20701 atctctgctg gtccgatccg tactctggcg gcctccggta tcaaagactt ccgcaaaatg
    20761 ctggctcatt gcgaagccgt taccccgatt cgccgtacag ttactattga agatgtgggt
    20821 aactctgcgg cattcctgtg ctccgatctc tctgccggta tctccggtga agtagtccac
    20881 gttgacggcg gtttcagcat cgctgcaatg aacgaactcg aactgaaata atcgttctgt
    20941 tggtaaagat gggcggcgtt ctgccgcccg ttatctctgt tatacctttc tgatatttgt
    21001 tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat ttcctttacg
    21061 accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg tatcatgaaa
    21121 cgcaatccag tactacggag tatgacgttc tgcctctggt cccggaagcc gcaaaggtca
    21181 gcgatccctt tctgctcgac gtgatccttg atgaagagac gctggccccc ttcctttcat
    21241 ggctggtccc tgcgcgcgtt cttgcagtgg agttgttccc tgaccagctt accgtgaccc
    21301 gttcacaaac cttcaccgct tatgaacgct tgtcgacggc cctgacggtt gctcaggttt
    21361 gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc cccggacctg
    21421 attcctccag ggaaagtaat catcggttgg cacaaatcac gcaatatgcc cgccaactgg
    21481 ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac gtcggtctta
    21541 ctgtctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt caggcgcgga
    21601 caattgcgac atttcaggct tatctcgggc atccggtacg ctggttaccc gggctggcga
    21661 tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg cgaagcagct
    21721 atgaggtgga aaaactacct gaagagcaca caaaaagttc aactgcagaa ctttgccaac
    21781 tggccgaaac actctctctc caccctattt cactttccct tctcgaaaag ttgttaaaca
    21841 gcacacgggt caatgcacag ccggataatc agcttgcggc gttgttatgc gcgcgtataa
    21901 atggcagtcc tgcttgtttt tccacctgta tggattcatc aaatgaatat aaaaaaatca
    21961 gcacccttct gcgcaagggc gaaaatgaaa ttaaccgatg ggctgaccgt cattctgttg
    22021 agcgcgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc tttagcgccg
    22081 cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt aatctgctgg
    22141 tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt gagacatatt
    22201 aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa
    22261 tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca actgcattcc
    22321 cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa
    22381 gtcgacgcac aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt catgcatggc
    22441 gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa
    22501 ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa tgaccgtctg
    22561 accatcgttc ctgatcatcc acttttaaaa gacgccattc cttgccgcgc agcccgtggc
    22621 ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg tcatccgctg
    22681 aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg tgacgaccac
    22741 tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc accagacggc
    22801 gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgacctc gctggatttt
    22861 gtcaccatcg acagtgccag cactgaagat atggatgacg cccttttcgc taaggcgctg
    22921 ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg gattgctgaa
    22981 ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct gcctggcttc
    23041 aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg cgccaatgaa
    23101 gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat tgaagataat
    23161 atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga ccaggtttct
    23221 gactggctgg aaaataccgg tgactggaag cctgaaagtg aagcaattgc cgaacaagtc
    23281 cgtttgctag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca cgcactggtg
    23341 tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc
    23401 gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat tgccgctaac
    23461 atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa cgtgcatatg
    23521 ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca cggtctgcat
    23581 gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg tgaactggat
    23641 gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt tgctgaaatt
    23701 agctctgaac ccggtcctca ctttggcctc ggtctggaag catacgccac ctggacttcg
    23761 ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt tatcaaaggc
    23821 gaaactgcga cgcgtccgca ggatgaaatc actgtccaaa tggccgaacg tcgccgtctc
    23881 aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct gaaagataaa
    23941 gccgggaccg gcacccgttt cgcggcggaa attgtcgata tcagccgtgg cggcatgcgt
    24001 gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt acacgctgtg
    24061 cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg tgaaacggtt
    24121 tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga aacccgcagc
    24181 attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg gatgggccgt
    24241 ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac tttcgtttga
    24301 aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaca atgcgtttag
    24361 ccgccgttaa tttcatgtaa gtcatgttgt tccttttatt taaccgcgga ctccgctgtt
    24421 aaccggagga tatgcatcat gaaaaccgtt agggagtcca caacgttgta caactttctc
    24481 ggatcgcaca atccatactg gcggttgacg gaaagcagcg atgctttgcg cttttctacc
    24541 accaaaacca cagaacctga tcgtacattg cagttatctg ccgaacaggc tgctcgcatc
    24601 agggaaatga cggtcatcac ctccagcctg atgatgagtc tgaccgtcga tgaaagcgat
    24661 ctttctgtgc atctggtagg acgaaaaatc aataaacggg aatgggctgg caacgcgtct
    24721 gcatggcatg acacaccggc ggttgctcgt gatttatcac acgggctttc ctttgctgag
    24781 caggtagttt ctgaagcaca ttccgcaata gtgattctcg acagccgggg gaatatccaa
    24841 cgcttcaatc ggttatgtga agattacaca gggttgaaag aacacgacgt cattgggtaa
    24901 agcgtgttta aactgtttat gagccgtcgt gaagctgcgg catccaggcg caataaccgt
    24961 gtattttttc gaagcggcaa tgcatatgaa gtcgaactgt ggataccaac acgtaaaggc
    25021 cagcggctgt ttctgtttcg caataaattt gtccacagcg gcagtggcaa aaacgagatt
    25081 tttttaatct gttccggtac cgacattacc gaagagcgcc gcactcagga gcgactgcgt
    25141 attctggcaa ataccgacag tatcaccgga ctgccgaatc gtaacgcaat gcaggagtta
    25201 atcgatcacg ctattaatca ggcagataac aataaagttg gggttgtgta tcttgatttg
    25261 gataatttta aaaaggtcaa cgacgcctat gggcatttgt ttggtgacca gttattacgc
    25321 gacgtgtcat tggctatttt aagctgtctc gaacatgacc aggtgttggc gcgtccaggt
    25381 ggcgatgagt ttctggtact ggcatccaat acctcacaaa gcgcgctgga agcaatggca
    25441 tcacgaattt tgacccgctt acgcctcccc tttcgtattg gtttaattga agtttatacc
    25501 agctgttcag taggtatttc gctctctccc gaacatggtt cggacagcgc gactattatt
    25561 cgtcacgccg acacagcaat gtacacagcg aaggaaggcg gacgaggaca attttgcgtt
    25621 tttactccag aaatgaatca gcgggtattt gaatatctct ggctggatac caacttgcgt
    25681 aaagcactgg aaaacgatca gttggttatc gaaaatcacc tagcgtgtcg aagtgcgcag
    25741 tctggaagca ctagtacgtt ggcagtcacc tgaacgtggg ttgattccac cgttggactt
    25801 catttcctac gccgaagagt cagggctaat tgtgccttta ggccgttggg tgattctcga
    25861 tgtcgtacgc caggtggcaa agtggcggga taaaggcata aacctgcgag tggcggtaaa
    25921 tatttctgca cgtcagctcg ccgatcaaac cattttcacc gccctgaaac aggttctcca
    25981 ggaactcaat tttgaatact gccctataga tgttgaactg acagagagtt gtctgattga
    26041 gaatgatgaa ctggcactgt ctgttattca acaatttagc caactaggtg cgcaagtgca
    26101 tctggatgat tttggtaccg gctactcttc actttcgcaa ctggcgcgct ttccgatcga
    26161 tgccatcaaa cttgaccagg tttttgttcg agatattcac aaacaacctg tctcgcagtc
    26221 actggtccgg gcgatcgtcg ctgtggccca ggcattgaat cttcaggtga tcgccgaagg
    26281 tgtagagagt gcaaaggaag atgctttttt aaccaagaac gggatcaatg agcggcaagg
    26341 atttttgttt gccaaaccga tgcccgccgt cgccttcgaa cgctggtata aacgctatct
    26401 gaagcgcgca taagtagatg aatatatata cttagcgcta tatcccgtca ttatatatcg
    26461 tattcctgga tgataattag ccgttgctgt taaacacttc tcttttagcg gcaatttttg
    26521 cattattcat tttccccctt ttgctataag gatgtccttg atgtctgaat ttgatgccca
    26581 gcgcgttgcc gaacgtattg atattgtgtt agatatcctt gtggcaggcg attaccactc
    26641 tgcgatccat aatctcgaga tacttaaagc agaactcttg cgccaggttg ctgaatctac
    26701 gccagacatc ccgaagacac cgtgggagat ttaacaatcg tgccttcagg ttttcctatt
    26761 tgaggatgcc cggcgcgtca gtattttttt atttagtatt ataacgttat aagaattaca
    26821 gcgatgaatt cccgacaaca aactattcta cagatggtca ttgaccaggg tcaggttagc
    26881 gtaaccgatc tggcaaaagc cactggagtt tctgaagtta ccattcgcca ggatctcaac
    26941 accctcgaaa aactgagtta cctccgccgt gcacatggct ttgcagtttc gcttgatagt
    27001 gatgacgtcg aaacccgtat gatgagcaac tatacgctga agcgtgaact cgccgagttt
    27061 gccgcgtcac tggttcaacc gggcgaaacc atctttatcg aaaatggcag cagcaatgcc
    27121 ctgctcgccc ggactctggg ggagcagaag aaaaatgtca ctatcatcac ggtcagcagc
    27181 tacatcgcgc atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg tggcgtgtac
    27241 cagaaaaaaa gcgaaagtat ggttggccct ttgacacgcc agtgcatcca gcaggtacat
    27301 ttcagcaaag catttattgg tattgatggc tggcaacctg aaactggatt caccggtcgc
    27361 gatatgatgc gcaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga agcgatagtc
    27421 ctgactgaca gctcgaaatt tggtgctgta cattcatact ccatcggtcc cgttgagcga
    27481 ttcaatcgcg tgattaccga ttcgaaaata cgcgccagcg atctgatgca tcttgagcac
    27541 agtaaactca ccgttcacgt cgttgacatt taaccatttc ccttctctac ggatgatttg
    27601 cagtttggca aatcatccgc tctaagatga ttcctggttg ataattaaga ctatttacct
    27661 gttattaaca ctctcaagat ataaaattat tatcagcgat ataacaggaa gtaattatca
    27721 cctgcgtgat ataaccctgc gcgcgagcag atttcacgga ataatttcac caggcttatt
    27781 cttagctatt atagttatag agagcttact tccgtgaatc ataaattcag gagagagtat
    27841 tatgtttgta acgagcaaaa aaatgaccgc ggctgttctg gcaattactt tggcaatgtc
    27901 tctgagtgcc tgttctaact ggtctaaacg ggaccgcaac accgcaatcg gcgcgggtgc
    27961 aggggcatta ggcggtgcag tactgaccga tggcagtacg ttgggtacat taggtggtgc
    28021 agctgtcggt ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa atgtcgatta
    28081 tcgactcgca attataatac gcttgattct gttcagtacg aaataactga ataatacaca
    28141 cagtaaaata ttcgaaagcc acggatatat ccgtggcttt ttatgttaac cgcctgcgag
    28201 ttttaccttc atccctttcg cttcaagtag tgattttaat aaatcacgct tatcgccctg
    28261 gatttcaata actccatctt taactgctcc gccgcagccg cattttttct tcagttccgc
    28321 tgcaagtttt gtcagttcgg catcatcgag atcgataccg gtaatcaggc aaacgccctt
    28381 acctttacgt ccactggtct gacgctgaat acgcaccgct ccatcgcctt tagggcgtac
    28441 tggggccgct ttgggctcat caatacgccc ggtttccgtt gagtagacca gacggctgtt
    28501 ggaatcactc atcatgcact ccgctgtaaa gaggcgttga tcgctttcag tgtctgcgct
    28561 ggatctaccg attgcgttac cgggcgacca atcaccatat aatcaacacc agccgccaac
    28621 gcctgttctg gcgtcataat gcggcgctgg tcaccagctt cactcccctg cggacgaatg
    28681 cccggtgtaa ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac agcttcctga
    28741 gcagaacaca ccacaccatc aaggtcacat ttttgcgtca gtgctgccag acgttctgca
    28801 taatctgcag gtgacagtgt cacgccaaga tcggccaggt cgctggcttc catgctggtc
    28861 aacactgtca cagcaatcaa aagcggtgca tctttgccaa acggaaccag tgcctcacgc
    28921 gctgcggtca tcatacgcgc cccaccggag gcatgaacat tcaccatcca cacgcctaag
    28981 tcagctgcag cagcgacagc gtgcgctgca gtgttgggga tatcgtggaa tttcaggtca
    29041 agaaagatat caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc aaacaatgta
    29101 aacatctctt taccgacctt cagacgacaa tcgcgtgggt cgatcttgtc gacaaaggac
    29161 agcgcgtcat cacaattatg ataatcaagg gcaacaacca caggagaatt cgtaacggcg
    29221 cgggaagaag atgaagcagt taacgtcatg accagacctt cttgatgatg ggcaccggga
    29281 gtggcgcgaa acaggtaaac tgcgagcatt ctacccgtga aaataaaaaa ttaacagttg
    29341 cgatttttcc cctagcaggc tttttctgct ccgtcgttgc cggagcacaa tgggacatca
    29401 ataactagta tgttgtaact aaagtcggat ttttttaaaa attacaggcc atcaagaccg
    29461 cgaatcggtt taatagttga ccaggcacga caagacggac aatgccagta gagggtgtat
    29521 gcggtaaaac cacatttctg gcagctataa cgaggcttac tacgcacctt ctcgccaacc
    29581 atgtcacgca gcaccatcag actctctttg gcgcgccctt cttccgcttc atttaagtgg
    29641 taatccatta gcttatggaa tacacgcatg gtcggatgac gctgaagctg gcgtgtaata
    29701 tagacctgtg cagcctcact accgtcgcgc gcttcgatga tatcagcaag catcaattca
    29761 gcatcggcac cggtgttctc ttccaccgcg cgttgcagga attctgccca ttcggcagtt
    29821 ttacccaact gctgatagca ggtttgcaac atttccagcg tttcgctgac cagttctctg
    29881 tcctgggata tgacgcgttg caaactttcg acggctttgg cgtattctcc tttcgccata
    29941 aacacgcgtc ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc cgcccctttt
    30001 tttagcaagg tcatggcacg atcgagatcg tcgctggcca tatgctgcag ggctaactca
    30061 cagtagaaat gggcaatttc gacgcgctgt ttatctttac ccagcttcac caggcgttcg
    30121 gcaacatcaa ttgctttctg ccactcactg gtagcctggt agatttgtag caactgttgc
    30181 agcgcgccaa tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc ttccgcgcgg
    30241 tcgtataacc cggcggccat gtaatcacgc cccagttgtt gaatcgccaa cagacgctgt
    30301 tcataggtca gcgaggcgct ttccattagg gtctgatgga tgcgaatagc gcgatcaact
    30361 tcgccacgcg aacggaacag gtttccgagc gtaaggtggg cttcaacggt gcctgtatcc
    30421 tctttaagca tatcgagaaa caggtctacc gctttatcct gttgattact aagcaggaag
    30481 ttaacccccg ctacgtaatc acgcgacaag cggttggctt catcttgctt gttttgttgc
    30541 gcacttctgc ggcccatata ccagccatag gcagcggcta caggcaaaag cagaaacaac
    30601 aactccagca tagaaagtta ttccttcgcc gctgacgagt gcggcactac agccacgtca
    30661 gtcgcgggtg aaagctggtt ttccagtcgc tttattttac gttcagcgcg cgccagggaa
    30721 acacgaactc gcagccagaa caggccacaa atcaaccaac cgatagcaaa ccccgcagca
    30781 aacaataccg ccagcaatgt ggaaatacgg tactcccctt gcgctaacag ataattaaac
    30841 gtcacctgtt gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat cgctaacacc
    30901 agtaagaaaa tgagtaaata tttcacatta cttcccgtta tgtggttaga gcgaataaat
    30961 tatggtcaac ttaccgcaat agccctttaa agtaccattt tcgcgccgtc tgcgaaatgg
    31021 catccacgcg cattgttgat gatttaaggc ctaaattcgg aaaatcaacc agcgttaact
    31081 ttcttgttct cgttgtgcaa tttcgcgatt ttcttccgca ggtggtgtta atggcccaca
    31141 aattcgttgc gcaagccacg ttgccaccgc caccagcgcc cacgaaatca acgtagctac
    31201 caccagatcg cgtggccaat gcatcccgag cagcaggcgg cttcccatga ctcccgttgc
    31261 ccagaccaac aagatagcaa tggttaacgt tcgccgacgc ggccacagca aaccaacggc
    31321 cagcagtgcc caactggcag caaacatcgt gtgaccggaa ggaaaggcaa accccgtctc
    31381 tttctgccag tgtgaacgca aatattgtgg aatatttttc tcttcagcca actgttcttt
    31441 tactagattt ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg gaatatgatg
    31501 tgttttttcc aaccagataa caaaaggtcg tggttcctgt actttgtctt tgatccagga
    31561 tttaacgcct tgtccaacaa ggattgccgc cgccagaatg gcaaataata caaaggcagc
    31621 cttaatgcga aaacgcagac accagagaaa ccagccgaac aaaatcaaat gtgtaatgac
    31681 gccccagggc tgggtgacag tttcagtaac ccaaaaagcc gcttttagta gccaactttg
    31741 ttctccaggt tgccaacgcc agccagaaat ccatacggct actggcatga caagcaatag
    31801 tgcagctccc actgcggtac gtcgggcaat cgaacgcatg gcctctcctt tttgataagt
    31861 cccacaatca taactgaaaa tgccagttcc aagaaaaatt gacagatttg tgccattccg
    31921 tgaacgatcg acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa ataagccaat
    31981 acagaaccag cattatctgg agaatttcat gcagcttaaa cgtgtggcag aagccaaact
    32041 gccaacccca tggggcgatt tcctgatggt gggatttgaa gaactggcaa ccggacacga
    32101 tcatgttgcg ctagtctatg gcgatatttc cgggcatacc ccggtacttg cgcgcgtcca
    32161 ttccgaatgt ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg gcttccagct
    32221 cgaagcggca ttgacgcaaa ttgccgagga aggccgtggt attttgctgt atcaccgtca
    32281 ggaaggtcgt aacattggtc tgctgaataa aatccgcgct tacgcactgc aggatcaagg
    32341 ttacgatacc gtagaggcta accaccagtt aggcttcgcc gctgatgagc gcgacttcac
    32401 tctttgcgct gatatgttca aactccttgg cgtcaatgaa gtccgcttgt taaccaataa
    32461 cccgaaaaaa gtcgaaattc tgaccgaagc agggattaat attgttgaac gcgtaccatt
    32521 gattgtaggt cgtaacccca ataacgaaca ttatctcgat accaaagccg agaaaatggg
    32581 ccatttgctg aacaaataac cctcttgcat cgtgtaattc atttgcttgc cggaagcaaa
    32641 ataaccggca agcaaatact tgttacttca acatattacg aatgacataa tgcaaaatgc
    32701 cgtcgttctg gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg cagggtacga
    32761 cttcctggct accatccgcg cgcgtaagcg tcaccggaac cgtcgcgcca ggttgtagat
    32821 tttgcagatc gccaatatca atcttctctt ccccggttag ccctaacgtt ttacgcgtca
    32881 cgccttgcgg aaattccagc ggcaggatgc ccatgccaat taaattcgaa cggtgaattc
    32941 gttcaaacga ttcggcaatc accacacgaa ttccaagcag acgaggtcct tttgccgccc
    33001 agtcacggct tgagcctgat ccatactctt tcccggcaat caccgccagc ggcgtttgct
    33061 cctgcttata gcgcatcgca gcatcataaa tagagactac gtcgctgtca ggtaaatgcc
    33121 gcgtcatccc cccttcaacg ccaggcacca tttcattacg gatgcgaata ttggcgaagg
    33181 tgccgcgcat catcacttca tggttaccac gccgcgaacc gtaggagtta aagtcttttc
    33241 gctcaacacc ccgaccttgt agatatcgac ccgctgggct gtcgggctta atactgcccg
    33301 ccggagagat atggtcagtg gtgactgaat cccccagcat tgcgaggatc cgcgcaccgt
    33361 gaatatcttc cactggtgct ggtgttgcct gcatttcatc aaagaaaggc gataagcgaa
    33421 tataggttga gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta atttccttcc
    33481 actctgctgt gccttcaaaa acttctgcgt actctttgcg gaacatttct gtggagactt
    33541 gttctaccgc acgggcaatt tcttgtgccg atggccagat atctttcaga taaaccggct
    33601 cgcctttgcg atcatggccg ataggctcag aagccaggtt gatattcata tttcccgcca
    33661 gcgcataggc aaccaccagc ggtggcgagg ccagccagtt agttttaacc agcggatgga
    33721 tacggccttc aaagttacgg ttgccggaca gcaccgcacc gacggttaaa tcgccttttt
    33781 tgattgccgt ttcgatagga tcgggcagcg gcccagagtt accaatacag gtggtacaac
    33841 cgtatcccac aaggttaaac cccagttcgt cgagatacgg tgtcagtttc gcttttgcca
    33901 gataatcaga aacgactttc gaacccggtg ccaacgacgc tttgacccac ggttgccgct
    33961 tgaggccaag agttacggct tttttcgcca gcaagcctgc ggccatcagc acacttgggt
    34021 tagaggtgtt ggtgcacgag gttatcgcag caatgaccac agcgccatca ggtaactgat
    34081 actgatgtcc gttcataaca taatcgactg gcaggcgatc tttatgcgtg gcattcactt
    34141 tcagttcgtt actggcggca aatgcttttg gtacatcggg cagtgcaacg cgatcctgtg
    34201 ggcgttttgg ccctgccagg ctcgcttcaa cgtcattcat atccagttct aacatactgg
    34261 taaaaatcgg ttcatcgccc gggttacgcc acatgccctg cgctttggca tatttttcga
    34321 ccaactcgac ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg gttactgcat
    34381 cgattgggaa gaagccacag gtggcaccat attctggcga catattggca atggtggcgc
    34441 gatccgccaa cggtagtgaa tccagaccat caccataaaa ttcgacgaat ttccccacca
    34501 cgccatgttt gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg gtaatacctt
    34561 cacgtaattt tccggtaagt ttaaagccca ctacgtccgg gataagcatg gaaaccggct
    34621 ggcctaacat ggcggcttct gcttcgatcc cacccacgcc ccaccccagc acgccaaggc
    34681 cgttgatcat agtggtgtgc gagtcagtac caacgagtgt atccggataa gcaatccatt
    34741 caccgtcctg caattcgctc cacactgctt tgccgagata ttcgaggtta acctgatggc
    34801 aaatgcctgt gcctggcggc acgacgctaa accgactgaa cgcttgcttt ccccatttca
    34861 ggaacacata acgttcgtgg ttgcgctcca tttccaggcg tacgttttct tcaaatgcct
    34921 catcatcacc aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg accggtgaga
    34981 gcgggttaac ctttgcagta tcgccaccga ggcgtttaac cgcttcgcgc attgccgcca
    35041 gatcaacaac ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc gggcggtagg
    35101 caatttcacg gtcagcatgg gcatttttca gccatcccgc cagtgcgtgg atatcctctt
    35161 cggtaactga gttaccatcc tgccagcgca gcaggttttc gagcaaaact ttgagtgact
    35221 tgggtagacg ggtgatatcg cccagtgatt tagcagcaag cggcaggctg tagtagtggt
    35281 aagttttatc tttggcctgc aacgtgtcct tactggcttc tcgtagggtt gacgacatag
    35341 ctcctcctta atgacagggt tgcgaagccc tgattctctt cagggtgaat attaaagata
    35401 acacaaacat cgcgtaacga tttgataaca acccaaattg ataaaagagg agaaaccttg
    35461 tggcaaagca aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt aatgatccag
    35521 aggagttcgg cccagaagag catcgacaga gaaaacgcgc ccatccagga ccaatatttc
    35581 atatttgtgt tgatcatagg ctacaccctt aacggttatt attttgtgtt gagataactc
    35641 aagctgttat tgctgcggtg tattaaaagc cctttaattc attaaaaatg ggtaataaga
    35701 atgatgataa ttattgtttc ggctgatcat cttcagcaaa cagttctata aaggcttgct
    35761 gctgcgatgt tagttgccag tcgtctggaa taacgacgga acattcccgt ttcgtttctg
    35821 gttcattgtc actatgctgg cataatggtg acgactccag ttgctcctga ccgaccatag
    35881 ttatccccac actttccaaa tcagcagtgc gacaaccacc cagaaaagtg cagaaccaag
    35941 aaaaaccgcc agccaggctt tacgttttaa ttcaggatca cggcggggtt cctgatttcc
    36001 agaaggcatt gctaacctca tataatcgcc atcacttatc agcaagacgt tgaatgattg
    36061 gtgtaattaa tcatacgatg agacaaatcc taaagaaact ttagtcaaaa atccagcgaa
    36121 tttacgaatc gtctccagtg aataattcaa tcttttgacc tgaaatagat atttgaaaac
    36181 tggaatttgc gaggcgggta attagacact actttacagg taatttaccc tataaatatg
    36241 agggataaaa atattgtgca cggtatagaa ataagtaaca aaagagaata tattccggca
    36301 ccttcgctac ataaaaggtg ccgaaaataa cgcaagaaat tatttttccg gcagttttat
    36361 atctttaaac atgacctcaa tttcttcatt ggagcgcaat gcgacagccg catcaacgac
    36421 atcacgcgtt aaatgcggtg caaaacgctg aatgaaatca tacatataac tgcgcaaaaa
    36481 agtactacgg cgaaaaccaa ttttggttgt actgtggctg aagatatcgt gagcatcaac
    36541 gcgcacaagg tcgggatcgg cgaccggatc caccgccatg ctggcaatga cccctacccc
    36601 cagccctaac cggacgtaag ttttaatgac gtcagcatcc gttgccgtga aaacgatacg
    36661 cggcgttaac cctgcgcggt taaaggcagt atccagttct gaacgtccgg taaagccgaa
    36721 ggtatatgtc accaacggat attgcgccag ttcttcaatg gtaatggctt ttttgcctgc
    36781 cagcgggtga tccggagtga ctacaatagc ccgattccag tggtagcacg gtaacatcac
    36841 taaatcttca tacagatgca gcgcttctgt ggcgatagcg aaatcggcat tgcctttaga
    36901 gacggcatca gcaatttgtg tcggcgggcc ctggtgcata tgcaaagaaa cgcggggata
    36961 acgctcaata aagcctttga taacgtttgg taacgcgtag cgtgcctggg tatgcgtggt
    37021 ggcgatatac agcgagcctt tatccggcca ggtgtgctca ccggcaaccg attttatggc
    37081 atcgactttc gacaggactt cgcgagcgat acgaattatt tcttgtcctg ctggcgttac
    37141 ctgcgtcagg tgcttgccgc ttcgggaaaa aatttgaatg cctagctcgt cttccagcat
    37201 tctgacttgt ttactgatcc cgggttgtga tgtgtaaagt ccttccgctg ttgatgagac
    37261 attcaggtta tgattgacca cctcaacaat atagcgaagt tgttgtaatt tcatgttaaa
    37321 ccatccactt agacgccatc agaacatcgt tttttcgtca cacctggcgg gtgactaata
    37381 cgatttaata ataaaaaagg tgctaactat aaccactata tcatttatag ccttagtgta
    37441 taggtgtgaa gaaaaaataa tatgcaataa aaaagggccg ctttcgcgac cctttgttta
    37501 taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc atcaacataa
    37561 aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg ctgtttggtt
    37621 ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg cgcatcggcc
    37681 agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc caccagtggc
    37741 gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaacacacc ggcagcaccg
    37801 tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa tggcaccgga
    37861 tcttctttcg gtggtgccac ttcaccgtta cgtaaaatct tacgtgtgtt tttacactct
    37921 tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat ttcagagcca
    37981 catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc gccctcttcg
    38041 atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg tttcggatcg
    38101 atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc gcgcagcgcg
    38161 ttggtttcag catcttcgcc ttccagcacg ttcagcactt cgttttccgg caccaggtta
    38221 atggtggttt tgcaacgctc tttcggcggc agcgcatagc cagaacagcc aaggaatacc
    38281 ccggtactcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc aatgctggtc
    38341 agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc tttatctaac
    38401 tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc ttcgtgattt
    38461 gccacctggt cgaggctgtt ttccatctgc gcggtaaagt cgtagttcat taactcgcgg
    38521 aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata gaaacgacga
    38581 ttttctactc gcacatagcc acgatcctga atggtcgaaa tgatcgacgc ataggtagac
    38641 ggacgaccga taccacgttt ttccagttct ttaaccagcg atgcttcact gaaacgggct
    38701 ggcggcttgg taaagtgctg ggctggtgtt agttcaacga gcgtcagagc atcgccttta
    38761 tcgactgccg gtaagatacg atcttcatcg cctttacgca gtgcaggcat cacttttgtc
    38821 cagccatcaa agcgcaaaat acgaccgcgt gctttcaggc ggaaatcgcc cgcaccaacg
    38881 gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggcaacgaa ctgacgccag
    38941 attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc cgccatcaca
    39001 ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt gctggcgtac
    39061 tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata accgcgaacc
    39121 atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt gatatagcct
    39181 gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaaacc aagacgggtg
    39241 ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact ggttgtcggt
    39301 ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac cgcagcctga
    39361 gtttgttctt tgttgaccgg acggaacggt ttgtcattct gatgagtcac ctgcaactcc
    39421 aacgcttcac cagatggcgt agtcgtgctg gcatcgactt cccagaactc ttccggtacg
    39481 aacgctttaa tttcacgctc acgctctacc accaggcgga ccgccaccga ctgcacacga
    39541 ccggcagaca gaccacgagc gatctttttc cacagcagcg gcgaaaccat ataccccacc
    39601 acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt cagctcaccc
    39661 ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac cactcggcta
    39721 tagcgcgcat catcaccacc aatcacttcc cgcaggtgcc atgcaatggc ttccccttcg
    39781 cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg tttcagttca
    39841 gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt gtgccacggg
    39901 tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt tttagccgtc
    39961 ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg caaatcgcgg
    40021 atgtgaccga cgctggattt caccacgtag tcacaaccca gatacttgtt gatcgttttg
    40081 gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt acctaatttg
    40141 attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag ttaccgatat
    40201 ggactcgctc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa aattcaggtg
    40261 actgagttaa caggtcttgg cctacaggcg ctatagcacg acggattttt ggtcgaatgt
    40321 caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa aatccgatac
    40381 gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc caccagcgtg
    40441 gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttgtttaa ggagtaaata
    40501 atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc caacaaaatc
    40561 attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac gcagcgtaat
    40621 ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc aacagccaaa
    40681 agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga aaaatatcat
    40741 ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta catcaatggc
    40801 ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc cgcgctgccg
    40861 gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac ttcacggcct
    40921 tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag gcctttctct
    40981 accgcctgtt gtccgtacca gtgttcaccc gttgccacct gttcaatatc cagagacgga
    41041 cgcatacgct tcacaaaatc tttaaataac tgatgcgttt cgttcagctc ttcgcggaat
    41101 ttctcccgcc cttcttcggt attttcaccc agcaaggtca gcgtgcgctt atactgcccg
    41161 gcggtgtgca gttcgatatc aatatctttg cttttcagga agcggttaaa gttgggcatt
    41221 tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat tttgtccgcc
    41281 acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac agttaaagga
    41341 atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc atgcaccatg
    41401 ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc tgcgagtacc
    41461 gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc tttaaaatcc
    41521 agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc tttcgctttt
    41581 gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg tttttgctga
    41641 tgtgagtcca tcagcgccgc ggccagttct tctttcatct ccttatactg ttcgctgaga
    41701 ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt gacaataatg
    41761 gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tagccaaaaa caaaccatat
    41821 tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct cacagtttac
    41881 aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg acaaagtatc
    41941 cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca ggcataaagc
    42001 cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga gtagccatgc
    42061 attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg ggagccagcg
    42121 atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca gtgattctgt
    42181 tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa gaaactgggc
    42241 gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat tgccaacaac
    42301 tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat aatgccggat
    42361 tgctcggcga tgtttgccca atgagcgaac aaaatccgca ggtctggcag gacgtcatgc
    42421 agatcaacgt taatgccacc tttatgctca cccaggcact gcttccttta ttactcaaat
    42481 cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga cgagccaact
    42541 ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta ctggccgatg
    42601 aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc accgcaatgc
    42661 gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct gatatcatgc
    42721 cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg acctttgacg
    42781 cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc aacagcgtca
    42841 gcagcgagtg aaagaaaaag tagatgctcg tgtggcccag gcccaggatg aacgcggtat
    42901 tatcatcgtc tttaccggca atggaaaagg caaaaccacc gcggcatttg gtacggcaac
    42961 acgcgcagtt ggtcacggaa aaaaagtggg cgtcgtgcag tttattaaag gcacctggcc
    43021 taatggcgaa cgcaatctgc tggagccaca tggcgttgag tttcaggtga tggcaacggg
    43081 ctttacctgg gatacacaaa accgcgagtc tgataccgcc gcctgccgcg aagtctggca
    43141 acatgcaaag cggatgcttg ctgattcctc actggatatg gttttgcttg atgaactgac
    43201 gtatatggtg gcgtatgact atttgccact ggaagaagtg gtgcaggcgt taaatgaacg
    43261 tccacatcaa cagacggtga ttatcacggg tcgaggctgt catcgggata ttcttgaact
    43321 ggcggatacg gtaagtgagc tgcgccccat cagacatgcg tttgatgccg gtgttaaggc
    43381 gcaaataggg attgattatt aatctgtaaa aatgccggat atgacgtccg gcatactttg
    43441 attaaccgtt attacgcccg ccagaacgac gactgccgct cacctgactg tgacgtttca
    43501 ccgcacgacg aatctgatta gccttcatgc gacgacggtc tttttctaca gcgactttag
    43561 agctggtttc tggcggaagc tccaccagtt cgcgcagata gttagtctgg gcgaggtcca
    43621 gctccgtcca gccaccgcgt ggcagacctt ttggcagtgg gatatcaccg taacgaacac
    43681 ggatcaggcg gctcacctgc acaccaaccg cttcccacag acgacgaact tcacggttac
    43741 ggccttcggt cagagtcacg ttgtaccact ggttgatccc ttcgccgccg ctgaacttga
    43801 tggttttaaa agctgccgga ccatcttcca gttgcacgcc acgactcaaa tcacgcagtt
    43861 tcgcgtcgtc aacctgacca aatacacgca cggcgtattc acgttcaact tcacggcttg
    43921 gatgcattaa acggtttgcc agttcaccat cggtggtgaa cagcagcaga ccacaggtgt
    43981 taacgtccag acgacctacg gcaatccagc gtgcgccacg cagctttggc agacggtcaa
    44041 acaccgtcgg acgcccttcc ggatcgttac gggtacacaa ctcaccttcc ggtttgtaat
    44101 aggccagaac gcgacaaatt tgttcagcgg actcacgtac cgaaatcaga tgaccatcga
    44161 tacgaatttt cagcccaggg gtaacttcaa cgcgatcgcc gagtttagca attttgccat
    44221 caacactcac acgaccggct tcaataatag attcgatttc acggcgggag ccgtggccag
    44281 cgcgggccag cactttctgt aatttttcgc tcatagagct tccttttggt gtcgccttca
    44341 caggcgtcga acaggagaat caatggcgcc gtatagcgcc attttaaggc cgcgtagtat
    44401 acagagttac gcacttataa gaaaggcttc acatcaccta cgccttcacg caccacgacc
    44461 ggcgtatcat cggtaagatc aataaccgtt gttggtttct gcccgagata gccgccgtga
    44521 ataatcaaat ctacctgttt ttccagacgg tctttaattt cttcgggatc ggattcggta
    44581 aattcgctgc ctggcagcat tagcgaagtg gaaagcatcg gttcgcccag tgcttcaagc
    44641 aacgcctgcg caatcgggtt agaaggcaca cgcataccga tggttttgcg tttctcctgc
    44701 aacagacggc gtggcacttc cttcgtcccc ttcaggatga aggtatagtt gcccggcgtg
    44761 ttgtttttca tcaaacgaaa cgcaacgtta tccacgaatg aataggtcga gagttcagaa
    44821 agatcgcgac acatcagggt aaagttgtga ccgtccggca gctggcgaat acgacaaata
    44881 cgctccatgg cgtttttatc ttcaatttta cagccgagcg catagccgga atcagttgga
    44941 tagacaatca ccccgccttt acgcacgatc tccaccgcct ggttgatcag acgttgctgc
    45001 gggttatcag gatgaatata aaaaaactgg ctcataattc cctctctgtg gtgttctgcg
    45061 gctgttccca tagctgccag acgccttcaa cgcctgcggg cagccagagt ttacgaccca
    45121 gttcgatcca cgggcatggc tgatgaaaat cagatccttg tgatgcccat aaatgatgct
    45181 gacgcgcaag tgtggccagc tgggtgcgtt cattgggcga ttgctggcac tgcgcgactt
    45241 ccatcgcgtc gccgtggtgt tcggcaaaat gcgcaaccaa tcttttcagc catttagcac
    45301 taagattgta ccgcccagga tgagctagca ccgccttacc gccagaatga tgaatgacat
    45361 caatagcttg ttctattgta caccactgtg gcggaacgta tccggttttc ccgcgcgcca
    45421 gatacttttt aaagacatct gccattgaac tggctttgcc gcactccaca aggaaacgcg
    45481 caaaatgacc gcgcgtcact gcgccgccct gcgccagtcg ttgtgcgcct tccagcgccc
    45541 caggtatttg cgctttttca agtctttcgg caatttgctg cgcccgctga ttgcgccgtt
    45601 ctgtctgctg cgcgaggaac tcacacatta gcggatgagt aatatcaata ttcagcccga
    45661 caatatgaat ttcatgattt tcccagaccg tggaaatttc cacgccggga ataagattca
    45721 gcgccagtcc cgaacgtgaa atttcctctc ttgctggcgc gattgcagct gtggtgtcat
    45781 ggtcggtgat cgccagggtg ccgacgcgca tctcgactgc acggtgcacc aatgcttctg
    45841 gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag gtcgtaaatc actgcataat
    45901 tcgtgtcgct caaggcgcac tcccgttctg gataatgttt tttgcgccga catcataacg
    45961 ggtctggcaa atattctgaa atgagctgtt gacaattaat catcgaacca gttaactagt
    46021 acgcaagttc acgtaaaaag ggtatcgaca atgaaagcaa ttttcgtact gaaaggttgg
    46081 tggcgcactt cctgaaacgg gcagtgtatt caccatgcgt aaagcaatca gatacccagc
    46141 ccgcctaatg agcgggcttt tttttgaaca aaattagaga ataacaatgc aaacacaaaa
    46201 accgactctc gaactgctaa cctgcgaagg cgcttatcgc gacaacccga ctgcgctttt
    46261 tcaccagttg tgtgggaatc gtccggcaac gctgctgctg gaatccgcag atatcgacag
    46321 caaagatgat ttaaaaagcc tgctgctgat agacagtgcg ctgcgcatta cagcattagg
    46381 tgacaccgtc acaattcagg cactttccgg caatggcgaa gccctgctgg cactactgga
    46441 taacgccctg cctgcgggtg tggaaagtga acaatcacca aactgccgtg tgctgcgctt
    46501 cccccctgtc agtccactgc tggatgaaga cgcccgctta tgctcccttt cggtttttga
    46561 cgctttccgt ttattgcaga atctgttgaa tgtaccgaag gaagaacgag aagcaatgtt
    46621 cttcggcggc ctgttctctt atgaccttgt ggcgggattt gaagatttac cgcaactgtc
    46681 agcggaaaat aactgccctg atttctgttt ttatctcgct gaaacgctga tggtgattga
    46741 ccatcagaaa aaaagcaccc gtattcaggc cagcctgttt gctccgaatg aagaagaaaa
    46801 acaacgtctc actgctcgcc tgaacgaact acgtcagcaa ctgaccgaag ccgcgccgcc
    46861 gctgccagtg gtttccgtgc cgcatatgcg ttgtgaatgt aatcagagcg atgaagagtt
    46921 cggtggcgta gtgcgtttgt tgcaaaaagc gattcgcgct ggagaaattt tccaggtggt
    46981 gccatctcgc cgtttctctc tgccctgccc gtcaccgctg gcggcctatt acgtgctgaa
    47041 aaagagtaat cccagcccgt acatgttttt tatgcaggat aatgatttca ccctatttgg
    47101 cgcgtcgccg gaaagctcgc tcaagtatga tgccaccagc cgccagattg agatctaccc
    47161 gattgccgga acacgcccac gcggtcgtcg cgccgatggt tcactggaca gagatctcga
    47221 cagccgtatt gaactggaaa tgcgtactga ccataaagag ctgtctgaac atctgatgct
    47281 ggttgatctc gcccgtaatg atctggcacg catttgcacc cctggcagcc gctacatcgc
    47341 cgatctcacc aaagttgacc gttattccta tgtgatgcac ctcgtctctc gcgtagtcgg
    47401 cgaactgcgt cacgatcttg acgccctgca cgcttatcgc gcctgtatga atatggggac
    47461 gttaagcggt gcgccgaaag tacgcgctat gcagttaatt gccgaggcgg aaggtcgtcg
    47521 ccgcggcagc tacggcggcg cggtaggtta tttcaccgcg catggcgatc tcgacacctg
    47581 cattgtgatc cgctcggcgc tggtggaaaa cggtatcgcc accgtgcaag cgggtgctgg
    47641 tgtagtcctt gattctgttc cgcagtcgga agccgacgaa acccgtaaca aagcccgcgc
    47701 tgtactgcgc gctattgcca ccgcgcatca tgcacaggag actttctgat ggctgacatt
    47761 ctgctgctcg ataatatcga ctcttttacg tacaacctgg cagatcagtt gcgcagcaat
    47821 ggtcataacg tggtgattta ccgcaaccat attccggcgc agaccttaat tgaacgcctg
    47881 gcgacgatga gcaatccggt actgatgctt tctcctggcc ccggtgtgcc gagcgaagcc
    47941 ggttgtatgc cggaactcct cacccgcttg cgtggcaagc tgcccattat tggcatttgc
    48001 ctcggacatc aggcaattgt cgaagcttac gggggctatg tcggtcaggc gggcgaaatt
    48061 cttcacggta aagcgtcgag cattgaacat gacggtcagg cgatgtttgc cggattaaca
    48121 aacccgctgc cggtggcgcg ttatcactcg ctggttggca gtaacattcc ggccggttta
    48181 accatcaacg cccattttaa tggcatggtg atggcagtac gtcacgatgc ggatcgcgtt
    48241 tgtggattcc agttccatcc ggaatccatt ctcaccaccc agggcgctcg cctgctggaa
    48301 caaacgctgg cctgggcgca gcagaaacta gagccagcca acacgctgca accgattctg
    48361 gaaaaactgt atcaggcgca gacgcttagc caacaagaaa gccaccagct gttttcagcg
    48421 gtggtgcgtg gcgagctgaa gccggaacaa ctggcggcgg cgctggtgag catgaaaatt
    48481 cgcggtgagc acccgaacga gatcgccggg gcagcaaccg cgctactgga aaacgcagcg
    48541 ccgttcccgc gcccggatta tctgtttgct gatatcgtcg gtactggcgg tgacggcagc
    48601 aacagtatca atatttctac cgccagtgcg tttgtcgccg cggcctgtgg gctgaaagtg
    48661 gcgaaacacg gtaaccgcag cgtctccagt aaatccggct cgtcggatct gctggcggca
    48721 ttcggtatta atcttgatat gaacgccaat aaatcgcgcc aggcgctgga cgagttaggc
    48781 gtatgtttcc tctttgcgcc gaaatatcac accggattcc gccacgcgat gccggttcgc
    48841 cagcaactga aaacccgcac cctgttcaat gtgctggggc cattgattaa cccggcgcat
    48901 ccgccgctgg cgttaattgg tgtttatagt ccggaactgg tgctgccgat tgccgaaacc
    48961 ttgcgcgtgc tggggtatca acgcgcggcg gtggtgcaca gcggcgggat ggatgaagtt
    49021 tcattacacg cgccgacaat cgttgccgag ctgcatgacg gcaaaattaa gagctatcaa
    49081 ttgaccgctg aagattttgg cctgactccc taccaccagg agcaactggc aggcggaaca
    49141 cctgaagaaa accgtgacat tttaacacgc ttgttacaag gtaaaggcga cgccgcccat
    49201 gaagcagccg tcgctgcgaa cgtcgccatg ttaatgcgcc tgcatggcca tgaagatctg
    49261 caagccaatg cgcaaaccgt tcttgaggta ctgcgcagtg gttccgctta cgacagagtt
    49321 accgcgctgg cggcacgagg gtaaatgatg caaaccgttt tagcgaaaat cgtcgcagac
    49381 aaggcgattt gggtagaaac ccgcaaacag cagcaaccgc tggccagttt tcagaatgag
    49441 gttcagccga gcacacgaca tttttatgat gcgctacagg gtgcacgcac ggcgtttatt
    49501 ctggagtgta aaaaagcgtc gccgtcaaaa ggcgtgatcc gtgatgattt cgatccggca
    49561 cgcattgccg ccatttataa acattacgct tcggcaattt cggtgctgac tgatgagaaa
    49621 tattttcagg ggagctttga tttcctcctc atcgtcagcc aaatcgcccc gcagccgatt
    49681 ttatgtaaag acttcattat cgacccttac cagatctatc tggcgcgcta ttaccaggcc
    49741 gatgcctgct tattaatgct ttcagtactg gatgacgacc aatatcgcca gcttgcagcc
    49801 gtcgctcaca gtctggagat gggggtgctg accgaagtca gtaatgaaga ggaactggag
    49861 cgcgccattg cattaggggc aaaggtcgtt ggcatcaaca accgcgatct gcgtgatttg
    49921 tcgattgatc tcaaccgtac ccgcgagctt gcgccgaaac tggggcacaa cgtaacggta
    49981 atcagcgaat ccggcatcaa tacttacgct caggtgcgcg agttaagcca cttcgctaac
    50041 ggttttctga ttggttcggc gttgatggcc catgacgatt tgcacgccgc cgtgcgccgg
    50101 gtgttgctgg gtgagaataa agtatgtggc ctgacgcgtg ggcaagatgc taaagcagct
    50161 tatgacgcgg gcgcgattta cggtgggttg atttttgttg cgacatcacc gcgttgcgtc
    50221 aacgttgaac aggcgcagga agtgatggct gcggcaccgt tgcagtatgt tggcgtgttc
    50281 cgcaatcacg atattgccga tgtggtggac aaagctaagg tgttatcgct ggcggcagtg
    50341 caactgcatg gcaatgaaga tcagctgtat atcgatacgc tgcgtgaagc tctgccagca
    50401 catgttgcca tctggaaagc attaagcgtc ggtgaaaccc tgcccgcccg cgagtttcag
    50461 cacgttgata aatatgtttt agacaacggc cagggtggaa gcgggcaacg ttttgactgg
    50521 tcactattaa atggtcaatc gcttggcaac gttctgctgg cggggggctt aggcgcagat
    50581 aactgcgtgg aagcggcaca aaccggctgc gccggacttg attttaattc tgctgtagag
    50641 tcgcaaccgg gcatcaaaga cgcacgtctt ttggcctcgg ttttccagac gctgcgcgca
    50701 tattaaggaa aggaacaatg acaacattac ttaaccccta ttttggtgag tttggcggca
    50761 tgtacgtgcc acaaatcctg atgcctgctc tgcgccagct ggaagaagct tttgtcagcg
    50821 cgcaaaaaga tcctgaattt caagcccagt tcaacgacct gctgaaaaac tatgccgggc
    50881 gtccaaccgc actgaccaaa tgccagaaca ttacagccgg gacgaacacc acgctgtatc
    50941 tcaagcgtga agatttgctg cacggcggcg cacataaaac caaccaggtg ctggggcagg
    51001 cgttgctggc gaagcggatg ggtaaaaccg aaatcatcgc cgaaaccggg gccggtcagc
    51061 atggcgtggc gtcggccctt gccagcgccc tgctcggcct gaaatgccgt atttatatgg
    51121 gtgccaaaga cgttgaacgc cagtcgccta acgtttttcg tatgcgctta atgggtgcgg
    51181 aagtgatccc ggtacatagc ggttccgcca cgctgaaaga tgcctgtaat gaggcgctac
    51241 gcgactggtc cggcagttat gaaaccgcgc actatatgct gggcaccgcg gctggcccgc
    51301 atccttatcc gaccattgtc cgtgagtttc agcggatgat tggcgaagaa accaaagcgc
    51361 agattctgga aagagaaggt cgcctgccgg atgccgttat cgcctgtgtt ggcggcggtt
    51421 cgaatgccat cggcatgttt gctgatttca tcaatgaaac caacgtcggc ctgattggtg
    51481 tggagcctgg tggtcacggt atcgaaactg gcgagcacgg cgcaccgcta aaacatggtc
    51541 gcgtgggcat ttatttcggt atgaaagcgc cgatgatgca aaccgaagac gggcagattg
    51601 aagaatctta ctccatctcc gccggactgg atttcccgtc tgtcggtcca caacacgcgt
    51661 atcttaacag cactggacgc gctgattacg tgtctattac cgatgatgaa gcccttgaag
    51721 ccttcaaaac gctgtgcctg cacgaaggga tcatcccggc gctggaatcc tcccacgccc
    51781 tggcccatgc gctgaaaatg atgcgcgaaa acccggataa agagcagcta ctggtggtta
    51841 acctttccgg tcgcggcgat aaagacatct tcaccgttca cgatattttg aaagcacgag
    51901 gggaaatctg atggaacgct acgaatctct gtttgcccag ttgaaggagc gcaaagaagg
    51961 cgcattcgtt cctttcgtca cgctcggtga tccgggcatt gagcagtcat tgaaaattat
    52021 cgatacgcta attgaagccg gtgctgacgc gctggagtta ggtatcccct tctccgaccc
    52081 actggcggat ggcccgacca ttcaaaacgc cactctgcgc gcctttgcgg caggtgtgac
    52141 tccggcacaa tgttttgaaa tgctggcact gattcgccag aaacacccga ccattcccat
    52201 tggcctgttg atgtatgcca atctggtgtt taacaaaggc attgatgagt tttatgccca
    52261 gtgcgagaaa gtcggcgtcg attcggtgct ggttgccgat gtgccagttg aagagtccgc
    52321 gcccttccgc caggccgcgt tgcgccataa tgtcgcacct atcttcatct gcccgccaaa
    52381 tgccgatgac gacctgctgc gccagatagc ctcttacggt cgtggttaca catatttgct
    52441 gtcacgagcg ggcgtgaccg gcgcagaaaa ccgcgccgcg ttacccctca atcatctggt
    52501 tgcgaagctg aaagagtaca acgctgcacc tccattgcag gggtttggta tttccgcccc
    52561 ggatcaggta aaagcagcga ttgatgcagg agctgcgggc gcgatttctg gttcggccat
    52621 tgttaaaatc atcgagcaac atattaatga gccagagaaa atgctggcgg cactgaaagt
    52681 ttttgtacaa ccgatgaaag cggcgacgcg cagttaatcc cacagccgcc agttccgctg
    52741 gcggcatttt aactttcttt aatgaagccg gaaaaatcct aaattcattt aatatttatc
    52801 tttttaccgt ttcgcttacc ccggtcgaac gtcaacttac gtcatttttc cgcccaacag
    52861 taatataatc aaacaaatta atcccgcaac ataacaccag taaaatcaat aattttctct
    52921 aagtcacata ttcctcaggt aattgttaat atatccagaa tgttcctcaa aatatatttc
    52981 cctctatctt ctcgttgcgc ttaatttgac taattctcat tagtgactaa ttttaatgag
    53041 tgtcgacaca caacactcat attaatgaaa caatgcaacg caacgggaga aataacatgg
    53101 ccgaacatcg tggtagttca ggaaatttcg ccgaagaccg tgagaaggca tccgacgcag
    53161 gccgtaaagg cggtcagcat agcggcggta attttaaaaa tgatccgcaa cgcgcatctg
    53221 aagcgggtaa aaaaggcggt caacaaagcg gtggtaataa atcaggcaaa tcctgattca
    53281 ccgttaatta ttaatatcag ctgataaaga taattgcatc tgcgggccgt atggctcgca
    53341 gtgatttcac tggagaaaaa tatgaatatg aagaccattg aagatgtatt tattcaactg
    53401 ctttcagata cctacagcgc agaaaaacaa ttaacccggg cactggcaaa actcgcaaga
    53461 gcaacatcaa atgaaaaatt aagtcaggct tttcatgcgc acctcgagga aactcatgga
    53521 cagattgaac gaattgatca agttgtggaa tcagaatcaa atctgaaaat taagcgcatg
    53581 aaatgtgtgg caatggaagg tcttattgaa gaagctaatg aggtcatcga aagtaccgag
    53641 aaaaacgaag tgcgtgatgc cgcactgatt gccgcagcac agaaagtcga gcattatgag
    53701 attgccagtt acgggacatt agcgacgctg gctgaacaat taggttaccg taaagcagcg
    53761 aagcttctga aagaaaccct ggaagaagaa aaggccaccg acatcaaact gactgatctg
    53821 gcccttaata acgtaaataa gaaagccgaa aataaagcct gaaatatgaa ttttaacttt
    53881 tagtcatttt ataaagagga cattttcatg aatcgtattg aacattatca tgactggtta
    53941 cgtgacgccc acgcaatgga aaagcaagcc gaatctatgc ttgaatccat ggccagccgt
    54001 atagataatt atcctgaact acgcgctcgt attgaacaac atcttagtga aaccaaaaac
    54061 cagattgttc aactggaaac tattcttgat cgtaatgaca tttcacgttc agtcattaaa
    54121 gattccatga gtaaaatggc tgcgcttggg cagtcaatcg gtggtatatt cccttctgat
    54181 gaaatagtta aaggctctat tggcggatat gtcttcgagc aatttgaaat cacctgttac
    54241 acctcactat tagcagcagc aaaaaatgcc ggtgatacag tctcaattcc gatcatcgaa
    54301 gcgattttaa atgaggaaaa gcaaatggcc gactggctga ttcagcatat tccgcaaaca
    54361 actgagaaat ttttaattcg ttctgaaact gatggcgtag aagcgaagaa ataataccct
    54421 ttataccatg tccttattga ccccgtatat tacggggtcg tttttgtgcg gaattaaaaa
    54481 cgatatcctg ctgagaacat aaacacccac ggatcgaggc gtacgctatc gtgttgctgt
    54541 gcaccgccta gcttataatt ggcggtggta tcgatatcca tgtaccacac tgacatgtta
    54601 accaaccagt cacggttaat cagataatca acccccacct gcccggcagc tccccaggaa
    54661 tctttcagac tgagatcgga aagccccgcc tctttgccat gatcgttaaa tccattatca
    54721 aagaaggtgg tgtagttaat accggcccca acgtaaggac ggaatttgct gctggcatca
    54781 ccaaaatacc actgcgccat cagtgttggc ggcagatggt gaacgattgc aatatcgccg
    54841 gtcgcccggg tgccgatttt atggcggaac ggcgtcgctg ccagtaattc cacaccaatg
    54901 ttgtcggtcg ccatataagt aaacgtcaag cccagttgag tgttattggt cacgctgaat
    54961 ccacccagac ttcctaacgt accaccagca ccttctgttg gacgtacggt tgcagaacct
    55021 gcacgcataa aaaattcgcc tgcttcatgc gcaaaggcac tgccagaaag aagagttgtt
    55081 actgccaaag ccgccactgt taactttttc atatccgctc cgtcgttatg gttataaaaa
    55141 cgtgacgaat atacctacat tggagtaaca aatgatccta catagatcac attacatatg
    55201 ataattcacc atttattgat ctggattaat ttagaagttg cattgagaat cctggttaat
    55261 gtgatttaaa tcaattttta aatattttct ctacgcaaat attaactcga tcgccacttg
    55321 ccgcgctttg cccacgcctg aaggccagca attacctccg ctttacaaag atatgctttt
    55381 ccatacaaga cctgatgctg ccacccgagg cttaaccagg gtacaattgc ccgctaatta
    55441 acacctgcat aaaactcaag gagagtgcat gtctatcacg gcgcagtccg tataccgtga
    55501 caccggaaat ttcttccgta atcaatttat gaccattctg ttggtatcgt tgctatgtgc
    55561 gtttatcaca gtggtgttag ggcatgtttt ctcacccagt gatgcacagc ttgcgcagct
    55621 caatgacggc gtgcccgtta gcggcagtag tgggttgttc gacctggttc agaatatgtc
    55681 accggaacag caacaaattt tgctgcaggc ttcagcggcg tccacttttt cagaattaat
    55741 cggtaacgcc attctcgccg gaggcgtaat attaattatc cagctggtgt ctgcgggtca
    55801 gagagtcagt gcgctgcgag ctattggtgc cagtgcgccg atattgccca agttatttat
    55861 tctgattttt ctgactaccc ttttagtaca gattggcatc atgctggtgg tcgttccggg
    55921 aattatcatg gccattttac tggccctggc accggtgatg ctggtacagg acaaaatggg
    55981 catttttgcc tcgatgcgta gcagtatgcg gctgacttgg gcgaatatgc gtctggtggc
    56041 acccgcagta ctgagctggt tgctggcaaa aacactgttg ctgctttttg cctcttcttt
    56101 tgccgcatta accccggaaa ttggtgccgt actggcgaac accttgagca acctgatttc
    56161 tgccatattg ctcatctatc tgttccgcct gtatatgttg attcgccaat aaccttaagc
    56221 atttgattac ggaatcgtaa aatgaagcag tttcttgatt ttttaccgct ggttgtcttt
    56281 ttcgcgtttt acaagattta tgacatctat gcggctactg cagcgctgat cgtcgccacg
    56341 gcgattgtgc ttatctatag ctgggttcgc tttcgtaagg ttgagaagat ggccctgatc
    56401 acttttgttc tggtggtcgt cttcggtggc ttgacgctgt tcttccacaa tgatgagttt
    56461 attaaatgga aggttacagt catttatgct ctgtttgcgg gagccctgtt agtcagccaa
    56521 tgggtgatga aaaagccgct aattcagcgg atgctgagta aagaactcac gctgccgcaa
    56581 ccggtatggt cgaagctaaa tctggcctgg gctgttttct ttatcctttg cggtctggca
    56641 aacatctaca tcgcattctg gctgccgcaa aatatttggg tcaactttaa agtctttggc
    56701 ctgaccgccc ttaccttaat cttcacattg ttaagcggta tctatatcta ccgccacatg
    56761 ccgcaggaag ataaatccta accagactgc cagtccttcg gggctggcgc tgtatctcct
    56821 ctgttctcct caggaaaatc atagtagcat cgcgcctgtg attttccttt taagtcggtt
    56881 ttaccatgtc tacaacacat aacgtccctc agggcgatct tgttttacgt actttagcca
    56941 tgcccgccga taccaatgcc aatggtgaca tctttggtgg ttggttaatg tcacaaatgg
    57001 atattggcgg cgctattctg gcaaaagaaa ttgcccacgg tcgcgtagtg actgtgcggg
    57061 ttgaaggaat gactttctta cggccggttg cggtcggcga tgtggtgtgc tgctatgcac
    57121 gctgtgtcca gaaagggacg acatcggtca gcattaatat tgaagtgtgg gtgaaaaaag
    57181 tagcgtctga accaatcggg caacgctata aagcgacaga agcattattt aagtatgtag
    57241 cggttgatcc tgaaggaaaa cctcgcgcct tacctgttga gtaatagtca aaagcctccg
    57301 gtcggaggct tttgactttc tgcttactga atttcggtgg tgccgttaat tttaaacagg
    57361 atattcacca caatcccact gcctggctta cccggctcat aacgccatct gcgcatcgca
    57421 tttttcacct cacgctcaaa catgttcgca ggcttggctg agaggatttg tacgttatcc
    57481 acgcgaccat ccggcgtgac gtcaaattta actttaacct gcccttcaat gcgcaatgcc
    57541 tgtgctcgtg ccggatactg cggctgatta cggcttaatg cgcgtggtcc tgaagccaca
    57601 ctggtaaccg gcttgctggt tgcagccgtt gccgtacttg atgtggggcg cgcgggtgcc
    57661 gtattttcaa acggtgatgc cggacgcgac tctacgggtt tgacatcgcg ttttggctgc
    57721 tcctgtacct ttttcaccgg cttcggtttt ggcttaggtt tcggcttcgg cttttcaatg
    57781 accaccggtg cttcttttgg cggttcgggg atcggctcag gttccggttc tggctctacc
    57841 accggctccg gtggcggctg aacggcttgt ggcggttcga gatcagcagg cgcaaccatc
    57901 gtgacagaaa ttggttgcgc aggcgcaggt agttcaataa cctgatgtac cgaggtatag
    57961 agcagccccg ccacaacagc accatgaatg cagaccgaaa gtaacgtcgg ccaggggaag
    58021 cggcgaggta aatcaagggt tattgaagtc ataatcattt cagtagaaaa actaggcctc
    58081 gattttaaat gcaaatagca atcatattca ataaggcaag acgatcagga tcagtgaaaa
    58141 tgtcattttt ggcgtaatta cccttacttt atgcatgacc ttaacaaaga catcatcttt
    58201 ccgttgcaga tcttcgccct ttcaaataac gtactttaca actttcccga acaaggagtt
    58261 gtgcccgtgt tgtatgttat ttacgctcaa gacaaagctg atagcctcga aaaacgcctt
    58321 tccgttcgtc cggcacattt agcacgttta cagttactgc atgatgaagg tcgtttgttg
    58381 acagctggtc caat
//
LOCUS       DAJTUP010000013        57308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTUP010000013 DAJTUP010000000
VERSION     DAJTUP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.013"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..280
                     /locus_tag="OO468_000824"
     CDS             <1..280
                     /locus_tag="OO468_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Stimulates the peptidyltransferase activity of the
                     70S ribosome and enhances dipeptide synthesis with
                     N-formylmethionyl-tRNA and puromycin in vitro, suggesting
                     its involvement in the formation of the first peptide bond
                     of a protein; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6258044.1"
                     /translation="QIEEELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAP
                     GIKGASASARNKPATLSTGLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            503..1969
                     /locus_tag="OO468_000825"
     CDS             503..1969
                     /locus_tag="OO468_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6258045.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2087..3073
                     /gene="yeiR"
                     /locus_tag="OO468_000826"
     CDS             2087..3073
                     /gene="yeiR"
                     /locus_tag="OO468_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR6258046.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3112..3825
                     /gene="lpxT"
                     /locus_tag="OO468_000827"
     CDS             3112..3825
                     /gene="lpxT"
                     /locus_tag="OO468_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR6258047.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4237..4803
                     /gene="mepS"
                     /locus_tag="OO468_000828"
     CDS             4237..4803
                     /gene="mepS"
                     /locus_tag="OO468_000828"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR6258048.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            4984..6540
                     /locus_tag="OO468_000829"
     CDS             4984..6540
                     /locus_tag="OO468_000829"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR6258049.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            6622..8436
                     /locus_tag="OO468_000830"
     CDS             6622..8436
                     /locus_tag="OO468_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR6258050.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            8437..9531
                     /locus_tag="OO468_000831"
     CDS             8437..9531
                     /locus_tag="OO468_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR6258051.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            9531..10556
                     /locus_tag="OO468_000832"
     CDS             9531..10556
                     /locus_tag="OO468_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6258052.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            10558..12147
                     /gene="yejF"
                     /locus_tag="OO468_000833"
     CDS             10558..12147
                     /gene="yejF"
                     /locus_tag="OO468_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR6258053.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12151..12495)
                     /locus_tag="OO468_000834"
     CDS             complement(12151..12495)
                     /locus_tag="OO468_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258054.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(12828..14018)
                     /gene="bcr"
                     /locus_tag="OO468_000835"
     CDS             complement(12828..14018)
                     /gene="bcr"
                     /locus_tag="OO468_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR6258055.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14046..14741)
                     /gene="rsuA"
                     /locus_tag="OO468_000836"
     CDS             complement(14046..14741)
                     /gene="rsuA"
                     /locus_tag="OO468_000836"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR6258056.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            14891..16651
                     /locus_tag="OO468_000837"
     CDS             14891..16651
                     /locus_tag="OO468_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6258057.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            16776..17060
                     /gene="rplY"
                     /locus_tag="OO468_000838"
     CDS             16776..17060
                     /gene="rplY"
                     /locus_tag="OO468_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR6258058.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17199..18206)
                     /gene="yejK"
                     /locus_tag="OO468_000839"
     CDS             complement(17199..18206)
                     /gene="yejK"
                     /locus_tag="OO468_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR6258059.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            18388..18615
                     /locus_tag="OO468_000840"
     CDS             18388..18615
                     /locus_tag="OO468_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR6258060.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            18635..20395
                     /gene="pbgA"
                     /locus_tag="OO468_000841"
     CDS             18635..20395
                     /gene="pbgA"
                     /locus_tag="OO468_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR6258061.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            20470..20546
                     /locus_tag="OO468_000842"
     tRNA            20470..20546
                     /locus_tag="OO468_000842"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20504..20506,aa:Pro,seq:ggg)
     gene            complement(20649..23240)
                     /gene="yejO"
                     /locus_tag="OO468_000843"
     CDS             complement(20649..23240)
                     /gene="yejO"
                     /locus_tag="OO468_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR6258062.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            23560..24207
                     /gene="narP"
                     /locus_tag="OO468_000844"
     CDS             23560..24207
                     /gene="narP"
                     /locus_tag="OO468_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR6258063.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(24418..25470)
                     /gene="ccmH"
                     /locus_tag="OO468_000845"
     CDS             complement(24418..25470)
                     /gene="ccmH"
                     /locus_tag="OO468_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR6258064.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(25467..26024)
                     /gene="dsbE"
                     /locus_tag="OO468_000846"
     CDS             complement(25467..26024)
                     /gene="dsbE"
                     /locus_tag="OO468_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR6258065.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26021..27964)
                     /gene="ccmF"
                     /locus_tag="OO468_000847"
     CDS             complement(26021..27964)
                     /gene="ccmF"
                     /locus_tag="OO468_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR6258066.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(27961..28440)
                     /gene="ccmE"
                     /locus_tag="OO468_000848"
     CDS             complement(27961..28440)
                     /gene="ccmE"
                     /locus_tag="OO468_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR6258067.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(28437..28646)
                     /gene="ccmD"
                     /locus_tag="OO468_000849"
     CDS             complement(28437..28646)
                     /gene="ccmD"
                     /locus_tag="OO468_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR6258068.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(28643..29380)
                     /gene="ccmC"
                     /locus_tag="OO468_000850"
     CDS             complement(28643..29380)
                     /gene="ccmC"
                     /locus_tag="OO468_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR6258069.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(29422..30084)
                     /gene="ccmB"
                     /locus_tag="OO468_000851"
     CDS             complement(29422..30084)
                     /gene="ccmB"
                     /locus_tag="OO468_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR6258070.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30081..30698)
                     /gene="ccmA"
                     /locus_tag="OO468_000852"
     CDS             complement(30081..30698)
                     /gene="ccmA"
                     /locus_tag="OO468_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR6258071.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(30717..31319)
                     /gene="napC"
                     /locus_tag="OO468_000853"
     CDS             complement(30717..31319)
                     /gene="napC"
                     /locus_tag="OO468_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR6258072.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(31329..31778)
                     /gene="napB"
                     /locus_tag="OO468_000854"
     CDS             complement(31329..31778)
                     /gene="napB"
                     /locus_tag="OO468_000854"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR6258073.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(31775..32638)
                     /gene="napH"
                     /locus_tag="OO468_000855"
     CDS             complement(31775..32638)
                     /gene="napH"
                     /locus_tag="OO468_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR6258074.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(32625..33320)
                     /gene="napG"
                     /locus_tag="OO468_000856"
     CDS             complement(32625..33320)
                     /gene="napG"
                     /locus_tag="OO468_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR6258075.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(33327..35813)
                     /gene="napA"
                     /locus_tag="OO468_000857"
     CDS             complement(33327..35813)
                     /gene="napA"
                     /locus_tag="OO468_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR6258076.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(35810..36073)
                     /gene="napD"
                     /locus_tag="OO468_000858"
     CDS             complement(35810..36073)
                     /gene="napD"
                     /locus_tag="OO468_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR6258077.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36063..36557)
                     /gene="napF"
                     /locus_tag="OO468_000859"
     CDS             complement(36063..36557)
                     /gene="napF"
                     /locus_tag="OO468_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR6258078.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            36666..36830
                     /locus_tag="OO468_000860"
     CDS             36666..36830
                     /locus_tag="OO468_000860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258079.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            36965..37453
                     /gene="eco"
                     /locus_tag="OO468_000861"
     CDS             36965..37453
                     /gene="eco"
                     /locus_tag="OO468_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR6258080.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(37575..38606)
                     /locus_tag="OO468_000862"
     CDS             complement(37575..38606)
                     /locus_tag="OO468_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR6258081.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(38840..39199)
                     /locus_tag="OO468_000863"
                     /pseudo
     CDS             complement(38840..39199)
                     /locus_tag="OO468_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(39417..41060)
                     /gene="yojI"
                     /locus_tag="OO468_000864"
     CDS             complement(39417..41060)
                     /gene="yojI"
                     /locus_tag="OO468_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR6258082.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41136..41786)
                     /gene="alkB"
                     /locus_tag="OO468_000865"
     CDS             complement(41136..41786)
                     /gene="alkB"
                     /locus_tag="OO468_000865"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR6258083.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(41786..42850)
                     /gene="ada"
                     /locus_tag="OO468_000866"
     CDS             complement(41786..42850)
                     /gene="ada"
                     /locus_tag="OO468_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR6258084.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(42924..43979)
                     /gene="apbE"
                     /locus_tag="OO468_000867"
     CDS             complement(42924..43979)
                     /gene="apbE"
                     /locus_tag="OO468_000867"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR6258085.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44091..45212)
                     /gene="ompC"
                     /locus_tag="OO468_000868"
     CDS             complement(44091..45212)
                     /gene="ompC"
                     /locus_tag="OO468_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6258086.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            45950..48622
                     /gene="rcsD"
                     /locus_tag="OO468_000869"
     CDS             45950..48622
                     /gene="rcsD"
                     /locus_tag="OO468_000869"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR6258087.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            48639..49289
                     /gene="rcsB"
                     /locus_tag="OO468_000870"
     CDS             48639..49289
                     /gene="rcsB"
                     /locus_tag="OO468_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR6258088.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(49489..52338)
                     /gene="rcsC"
                     /locus_tag="OO468_000871"
     CDS             complement(49489..52338)
                     /gene="rcsC"
                     /locus_tag="OO468_000871"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR6258089.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(52613..53389)
                     /locus_tag="OO468_000872"
     CDS             complement(52613..53389)
                     /locus_tag="OO468_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR6258090.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(53394..55043)
                     /locus_tag="OO468_000873"
     CDS             complement(53394..55043)
                     /locus_tag="OO468_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR6258091.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55044..55603)
                     /locus_tag="OO468_000874"
                     /pseudo
     CDS             complement(55044..55603)
                     /locus_tag="OO468_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(55762..>57308)
                     /locus_tag="OO468_000875"
     CDS             complement(55762..>57308)
                     /locus_tag="OO468_000875"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6258092.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14013 a        15481 c        14073 g        13741 t
ORIGIN      
        1 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
       61 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
      121 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
      181 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
      241 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
      301 gggatagctc aggctgtccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
      361 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
      421 taacaatcca gaccaacttc aattgctgtc atataacttt acactgtcat tgttaattta
      481 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
      541 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
      601 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
      661 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
      721 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
      781 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
      841 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
      901 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
      961 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
     1021 tgagcgcagc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
     1081 tgtggtgaaa aacgcggtgc tgagaatggc agaaaaacgt tcgccagaac tcgccgggtg
     1141 gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac
     1201 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
     1261 ctgcgaaccg tttatccagt gggtggttga agataacttc atcgctgggc gtcctgcctg
     1321 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
     1381 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctctcag gattcgccta
     1441 tatcagtgat tgcatgcagg atcgcgtatt tcgctatgct gccagaacat taatgctgga
     1501 tgagcaagcg ccgacactgc aaattaaaga tgtcgattta acacaatatg cggataagtt
     1561 aattgcacgt tttgctaatc cgtcgctgaa acataagacc tggcaaatcg cgatggatgg
     1621 cagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgtgaaac
     1681 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
     1741 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
     1801 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
     1861 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
     1921 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
     1981 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
     2041 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
     2101 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
     2161 taaagatccc aacgaaaaat gggcggtact ggttaatgaa tttggggaag tcggaattga
     2221 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
     2281 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
     2341 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
     2401 aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc
     2461 acgcctgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
     2521 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
     2581 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
     2641 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
     2701 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
     2761 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
     2821 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
     2881 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
     2941 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
     3001 ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc
     3061 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
     3121 aatttgccgc aaatagtgtt gttgaatatt gtcggccccg cgctgtttct ttcctggtat
     3181 attcccgtta atcatggatt ctggttgccg attgatgcgg atatttttta tttctttaat
     3241 cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgactaa caatcgcgcc
     3301 ttcgacggtt gttcactgct ggcgatgggt atgttgatgc tgagtttctg gctgaaagaa
     3361 aacgcccctg gcagacgacg tatcgtgatt atgggtctgg tcatgctatt aactgcagtg
     3421 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgact
     3481 tttaccgata ttaaccgcgt cagcgaactg ctctctgttc ccacgaaaga tgcctcacga
     3541 gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
     3601 tatttcggca aagttgcagg ccttatcgcc cttattattt ttatggtttt tgcatttccc
     3661 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
     3721 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
     3781 gacaaatcac taccaggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
     3841 atcatcaggg ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct
     3901 atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaattg
     3961 tactttaatt gaccaacccc gcttattaac tttctgtatc actttttctt ataaaaaatc
     4021 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg
     4081 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
     4141 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
     4201 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
     4261 agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
     4321 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
     4381 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
     4441 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
     4501 aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta
     4561 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
     4621 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
     4681 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
     4741 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgggttct cagccgcagc
     4801 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
     4861 taggcagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
     4921 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
     4981 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
     5041 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
     5101 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
     5161 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
     5221 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
     5281 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
     5341 ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
     5401 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
     5461 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
     5521 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
     5581 cgtttgatga acgttaatga attaaccgac atgccggtcc atgaaactaa aattgcgggc
     5641 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
     5701 tttttactgg gcggcatgag tggaactgtc gttggcctgc tttgctatta cctgatgagc
     5761 gtacgtatgc gccccgacag agaaatcatg accgccatca agcgcgaaca attttacgtg
     5821 gcgtatcaac cggtggtgga tacacaagct ttgcgggtaa cgggcctgga agtactgcta
     5881 cgctggcggc atcctgtagc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
     5941 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
     6001 gcagaattag aaaaagtact gccggtaggc gtcaaatttg gcattaacat tgcgccggac
     6061 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
     6121 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
     6181 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
     6241 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
     6301 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
     6361 ctgacgctgg cgaaacgtct caatatgctg acagttactg aaggggtcga aacgccggaa
     6421 caagcgcgat ggctaagcga acgcggcgtt aatttcatgc aaggctactg gattagtcac
     6481 ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa
     6541 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
     6601 gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat cgctctgttc
     6661 accttcggtg cgcaggcgca ggctatcaag gaaagctata cctttgccgt gctgggcgaa
     6721 ccccggtacg cgtttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
     6781 gggcagataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc
     6841 ggcaacccgg gcgcacgcac cgagcagctg tacgacacgt tatttacgac ttccgatgac
     6901 gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc
     6961 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttctcc cattactgcc
     7021 cgcgatgtag agtttacttt tcaaaaattt ctgaccgaag gcgtgccgca atttcgtctg
     7081 gtctacaaag gcaccaccgt caaagccatt gcgccgttaa ccgtgcgcat tgagttagct
     7141 aaacccggca aagaagatat gttgagtctg ttttcgctgc cggtatttcc agaaaagtac
     7201 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
     7261 cacgttacgt cctggaaaat ggggcaaaat attgtctatt cccgcgtgaa agattactgg
     7321 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
     7381 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
     7441 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
     7501 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
     7561 atccaacgtg cggtattcag cgatcgccgg gtgcgggaag caatcactct cgcctttgac
     7621 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
     7681 caaaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
     7741 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
     7801 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acaaacttct caacgaagcg
     7861 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagcc gctcagcttt
     7921 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg
     7981 caacggctgg gtatcaatat ggacattcgc aaggtggata actcgcaaat cactaaccgc
     8041 atgcgcaatc gcgactatga catgatgccg cgactatggc gggcgatgcc gtggcccagt
     8101 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
     8161 gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa
     8221 gaaaaattac tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
     8281 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccaaccc
     8341 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
     8401 gccaaactgc cgtcagccag acaacaggga gagtagatgg gcgcttacct gattcgccgt
     8461 ctgttgctgg tgatcccaac gttatgggct attatcacta tcaacttttt catcgtgcaa
     8521 attgcgcctg gcggtccggt cgatcaggcc atcgccgcca ttgagtttgg taatgccgga
     8581 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
     8641 aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag
     8701 atcactcatc gctacggttt cgataagccg atccacgaac gttacttcaa aatgctctgg
     8761 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
     8821 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
     8881 ctggtgtcga ttccgttagg cattcgcaaa gctgtttata atgggagtcg ctttgacgtc
     8941 tggagtagcg catttatcat catcggctac gccattccgg cctttttgtt tgccatcctg
     9001 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
     9061 tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc
     9121 acgctaccgg tgctggcgac agtgattggt ggctttgcgg cgctgaccat gctgaccaaa
     9181 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
     9241 agtgagaaaa acattctctg gaaacatatg ttccgcaacg ccatgctgct ggtgattgcc
     9301 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
     9361 ttttcactca atggtcttgg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
     9421 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
     9481 gatatcagct atacgctggt tgatccgcgt atagattttg agggacgtta atgtcgcgac
     9541 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
     9601 cgttatggat tttcctcgtt ttgtttggtt tgagtttgtg ttctgaactt atcgctaacg
     9661 ataaaccgtt gctggtgcgt tatgacggca gttggtattt cccgttgttg aaaaactaca
     9721 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
     9781 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
     9841 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
     9901 gaacggatgc aaacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
     9961 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    10021 tacaaggcta ttacggcggt aaagtcgatc tctgggggca acgctttatt gaagtatggt
    10081 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    10141 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    10201 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcacaggcg cttggcgtca
    10261 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    10321 ttttaccgtt tattttatgt agttcgataa ccaccctgac ctcgctcgat ttcctcggct
    10381 tcggtctacc gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc
    10441 ttcaggcccc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    10501 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    10561 acgcaaactc tgttagcgat tgaaaatttg tcggtgggtt ttcgccatca gcaaaccgta
    10621 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    10681 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    10741 ccgccggttg aatatctctc cggcgatatt cgttttcatg gcgaatcgct gcttcatgcc
    10801 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaggaaccg
    10861 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    10921 caccgcggga tgcgtcggga agcggctcgt ggcgaaattc ttaactgcct tgatcgcgtc
    10981 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    11041 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    11101 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    11161 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    11221 aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaataacgcc
    11281 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    11341 tcaggcgatc cggtaccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    11401 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    11461 gtgaaaaaca tcagttttac gctacgggcg ggtgaaacac tgggtttagt gggcgagtct
    11521 ggttccggga aaagcacgac gggactggcg ctgctgcgac tgattacctc tcagggcagc
    11581 atcgtctttg acggtcagcc actgcaagat ttaaatcgcc gccagctgtt acctactcgt
    11641 catcgtattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    11701 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    11761 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    11821 tatccggcgg agttctctgg tggtcagcga caacgtattg cgattgccag ggcattaatt
    11881 cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    11941 gcgcaaatat tgacgctttt gaaatcattg caacaaaagc atcaactggc ctatttgttt
    12001 atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    12061 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccacaccgca gcaggagtat
    12121 acgcgtcagt tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    12181 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    12241 tacaaacagg cacggctcac cttcacactc caacaccgaa catggaactt cgatgtcgct
    12301 taacgcctgg cttagtttat acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    12361 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgaggaatcg gttcaacctt
    12421 cgaacctgcg aaaccagcag accagcgata gttctgcggc agtcgatgga cgattgagag
    12481 ttgtaatgaa gtcacttatt ttccccggaa gtacattcac ttcacaattt gtttacatca
    12541 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    12601 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    12661 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    12721 acataaattg attttacata aaataaacat atatcgggga aatgatagat ttgtggttga
    12781 catgcatcaa caaaggaagc cttttacctt cctcgttgtg caatagatca ccgttttttc
    12841 ggccgactgg cgtacagaca gaagagaatg gagctggttg cgcagaatgc aattgaccaa
    12901 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    12961 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    13021 tcaagaatga ccgccatcgc attggatgac accatcgaca cgcagcccac aaacgccgca
    13081 acgccaacca ccagcgacca gaatcccagc cccagcggcg cactgatgac catccacgca
    13141 gccataataa attgtatcca caaccccgag cggaacatat ttaacgcgcc aatgcggcgg
    13201 acgaagcggc tgttaaagat ggtcatcacg aacagaaaaa caatgtttag cgcaaagtaa
    13261 taaccaaagt tttccggcgc gacgtggtta atttcaatat aaacaaacgg tccggcgctt
    13321 aagaatgaga acatcccggc aaagctgaaa ccactggcaa gcatgtagct caggacacgt
    13381 ttatggcgga acagcgccgc aaagttacca atagtggtac gaatgtgaaa tggctgacga
    13441 cgctccggtg gtaaggtttc tttaatcagg aagaaaatca ttgccgaagc cagaatcgcc
    13501 gctaatgcca ggatccagaa gatgtaatgc cagctcagcc acaccagcac ccagccgcca
    13561 actatcggtg ccatcagcag tgcaatggtc gtcaccagca tgacaaacga catcatccgc
    13621 gagaactctt ctttcgggta aatatcacgc atcaaagcgt tgataaccac gctggccgca
    13681 gccgcagcca gcccgtggaa gaaacgcatc acaatcagct gatcgatggt ttgtgccaac
    13741 gcacacgcca ccgcggcggc ggcaaacacc agcgtcccgc caagaaccac cggcttacgc
    13801 ccgaagctgt ctgccatcgg cccgtagatt aactgcccca acgcaaagcc cagaatataa
    13861 gtactgaggg tcatctgcgt actgcccgcc gatacgccaa actgcgctga aattaccggt
    13921 agcgcgggca gatacatatc aatcgacagc ggcatcaaca tggccagcag gccaaggata
    13981 aaaacaatag caaacgacga atgctgtcgg gtggtcacaa cgggctcctg aaagtcattg
    14041 aaaagttaga cgacgctggc aatttcttct tcagttaacg gacgatattc accgggggct
    14101 aaatcagcat ccagcgtaat accgccaata cgttcacgat gcagctcaac cacgtggtta
    14161 cccacggcgg cgaacatgcg tttcacctga tgataacgcc cttcgctgat ggtcagacga
    14221 acctgcgttg gggtaatcac ttcaagcacc gccggcttag tgagatcttt ttcgttatgc
    14281 agctgcacgc ctttagcaaa ttgctctgcc gtatcgtcag ctacaggtga ttccagtgtc
    14341 accagatagg tcttctcgca atgatggcgc ggagaagtaa tgcggtgcga ccactgacca
    14401 tcatcagtca tcagcaccag accggtggta tcgatatcca gacgtcctgc ggcgtgcagt
    14461 ttccacgcta ccggttcatc cagaaaatag agcaccgtcg ggtgatccgg gtcgtccgtg
    14521 gagcaaacat agccctgagg cttattgagc atgaagtaac gtggaccgtg ttgctgcgcc
    14581 agcgggttgc catcgtaagc gacatcatgt tcagggagca gtttgaacgc tgcattacga
    14641 acgatttcgc catcgacggt gacacgattg ccgcggattt cacgcccggc aatagcacgg
    14701 ctaacgccga gttgctgtgc gataaattta tcaagtcgca tgtgtgtgat tttgcctgta
    14761 aaaaacggag gtcgggtaat gacccgaaaa tctgaactgt tgtctgccca gtatagcggt
    14821 ctaataactt ccctcaaggg aaaaagaacc gtggcatact attagcagaa taatctacct
    14881 acgcgaaacc atgattttta cactccgccc atatcagcaa gaagccgtgg atgccacgct
    14941 caaccatttt cgtcgtcata aaacccctgc cgttatcgtg ctgcccaccg gcgcaggtaa
    15001 aagcctggtg atagcggaac tggcacggct ggcgcgtggt cgcgtactgg tgctggcaca
    15061 cgttaaagaa ctggtggcgc aaaaccatgc aaagtatcag gcgctggggc tggaagccga
    15121 tatttttgcc gccgggctaa agcgcaaaga gagccacggt aaagtggtat ttggcagcgt
    15181 tcagtcggtc gcccgtaatc ttgatgcctt tcagggtgaa ttttcgctgt tgattgtcga
    15241 tgaatgccac cgtattggtg acgatgaaga gagccagtat cagcaaatcc tcactcacct
    15301 gactaaagtg aatccccact tacgcctgct ggggctgact gccacgcctt ttcgattggg
    15361 caaaggctgg atctaccagt tccattatca cggcatggta cgcggcgatg agaaagccct
    15421 tttccgtgac tgcatttatg agctgccgct gcgttatatg attaaacacg gctatctgac
    15481 gccgccagaa cgactggata tgccagtagt gcaatacgat ttcagccgct tgcaggcaca
    15541 gagtaacggg ctgttcagcg aagctgatct caaccgtgag ctgaaaaaac agcaacgcat
    15601 tacgccacat atcatcagcc agatcatgga gttcgccgaa aagcgcaaag gggtaatgat
    15661 tttcgccgcc accgttgaac acgcaaaaga gattgtggga ttactgcctg ccgaagatgc
    15721 tgcactgatt actggcgaca accccggcgc tgagcgcgat gtgttaattg aagattttaa
    15781 agcccagcgt tttcgctatc tggtcaacgt cgcagtactg accaccggat ttgacgcccc
    15841 gcacgtcgat cttatcgcca ttctgcgccc taccgaatca gtgagtcttt accaacaaat
    15901 tgtcgggcgc ggtctgcgtc tcgctccggg caagactgat tgtttaattc ttgattatgc
    15961 gggtaatcct cacgatctct acgcgccgga agttggtaca ccgaaaggca aaagtgacaa
    16021 cgttccggta caggttttct gccctgcctg cggttttgcc aacacctttt gggggaaaac
    16081 gaccgccgac gggacattga ttgaacactt tggtcgccgc tgtcagggat ggtttgaaga
    16141 tgacgacggt catcgcgaac agtgtgactt ccgtttccgt tttaaaaatt gcccgcaatg
    16201 taacgcagaa aatgatattg ccgctcgccg ctgccgcgaa tgtgacacgg tactggttga
    16261 tccggatgat atgttaaaag cggcgctacg actgaaagat gcgctggtat tacgctgtag
    16321 cggcatgtct ttgcagcatg ggcacgacga gaaaggcgaa tggttgaaaa tcacctatta
    16381 cgatgaagac ggcgcggatg tgagtgagcg tttccgtctg caaacgcccg cccagcgtac
    16441 cgccttcgag cagcttttta tccgcccgca tacgcgcaca ccgggcatcc ctctgcgctg
    16501 gatcaccgcc gccgatatcc tcgcccagca agccttactg cgacatccgg attttgtcgt
    16561 cgcccgcatg aaaggtcagt actggcaagt gcgtgaaaaa gtgttcgatt acgaaggtcg
    16621 ttttcgtcgg gcgcacgaat tacgcggtta atggtagttt tgattgatgt ataaggcgat
    16681 tgagtataga atctcgcccg cttttgcata cgcaaagcag atcacttacc tgttgctggg
    16741 tcgcctgtag caggattaat ttaagagaga aagaaatgtt tactatcaac gcagaagtac
    16801 gtaaagagca gggtaagggt gcgagccgcc gcctgcgtgc cgctaacaag ttcccggcaa
    16861 tcatctacgg tggcaaagaa gcaccgctgg ctatcgagct ggatcacgac aaagtcatga
    16921 acatgcaagc taaagctgaa ttctacagcg aagttctaac catcgttgtt gacggtaaag
    16981 aaatcaaagt taaagctcag gacgtacagc gtcacccgta caaaccgaag ctgcagcaca
    17041 tcgacttcgt tcgcgcttaa ttgctgaata agttgtaaaa aaccccgctc cggcggggtt
    17101 ttttgtatct gcagattatg cctgattacg gtattgctat tttttgcagg ccagataagg
    17161 cgttcacgcc gcatctggca ttcagaacac aaaaccgatt aattgccgcc agacgtccgg
    17221 cgctgcaatt ggtcgcgcaa attcggcggc gtgcctttaa tggtcaaggt atcggttgct
    17281 ggatcccaga agatgcgttc acctaacagc atcgcatcaa agttaatagt caagccaccg
    17341 ccgctaccgg caaacttcgt caactgacgc agtgtgctac ggtctgccgg gaagctctct
    17401 tccagttcgt agcctttttc agccgcaaac tccgtgaagc tcacttcgct tacgcctgcc
    17461 agctctttcg acagcgattc cagttcgatc tcttcgcctg cctgcagctg ctcattacag
    17521 tagctataaa cctgctggcg cacgttctgt cgctctgctt tatccagttg tgcttcggca
    17581 gtgaaatcat caaccgcttg caacagccca cggttttgcg ctttcgcgtt caatccttcg
    17641 ctggccccga ggaaatccat aaagaaatcc gccactttgc gccctactcg ccctttcagg
    17701 aaagtgagat agcgggtgga ctctggattg gtttcccatt cggttaaatc gatacgcgca
    17761 acaatatccg catgattgat atcaagataa tgggttgggt tgatgtcgag attttcgtta
    17821 acgcgcatac tgctcaggtt gctcagaact gccaccagca aatactccac cgccagataa
    17881 cggtagtggc aaaacagcac gaatccaccg tcagcaaaag gatatttcgc taattcgtca
    17941 cgcaggcgac cggttgccgc gcggctaaat gccagaaagt cctcttcgcc ctgacgctgt
    18001 aagcgcagcg tctgcgccag ttcactctct tcgctaaaca agccgtaagc tttatttttg
    18061 gcgctataga cccgatgcag ttctgccacc atctcaacga cggtttctgt cggttccagc
    18121 aatgaatcgc gcaacaccag ctcaaggttc tgctcatcac gcttgataag ctggtgcaga
    18181 gcaatctggt tgatatccag actcatgata aactctcctt taagaccggg cggtattcaa
    18241 ccaccgccag ttaagcgacg caataaaaga tggcggtcat tttacagaag gcagcgtaac
    18301 gagaatgatt taacgcataa aaaagcagaa aaaaaaccgt tgctacggta atatgttgcc
    18361 ctttcatcaa ctaactgatt tcgatttatg ccacaaattt cccgctacag taatgaacag
    18421 gttgaacaat tgctcgcaga gctgctcaac gtactggaaa aacataaggc tccgaccgac
    18481 ctttccctga tggtgttagg caacatggtc actaacctta tcaacaccag cattgccccg
    18541 gcccaacgcc aggcaattgc caactctttt gccagcgcct tacagtcctc tatcaacgaa
    18601 gacaaagcgc actaagggaa acagataaca ggttatggta actcatcgtc agcgctaccg
    18661 tgaaaaagtc tcccagatgg tcagttgggg gcactggttt gcactgttca atattctgct
    18721 ttcgctcgtc attggcagcc gttacctgtt tatcgccgac tggccgacaa cgcttgctgg
    18781 tcgcatttat tcctacgtaa gcattatcgg ccatttcagc ttcctagtgt tcgccaccta
    18841 cttgctgatc ctcttcccgc tgacctttat cgtcggctcc cagaggctga tgaggttttt
    18901 gtccgtcatt ctggcgacgg cgggaatgac gctattactg atcgatagcg aagtctttac
    18961 tcgtttccat ctccatctta atcccatcgt ctggcagttg gttatcaacc cagacgaaaa
    19021 tgagatggcg cgcgactggc agctgatgtt catcagcgtg ccggttattt tattgcttga
    19081 actggtgttt gcgacgtgga gctggcaaaa gctgcgcagc ctgacgcgtc gtcgacgctt
    19141 cgcgcgcccg ctggccgcat tcttatttat cgcctttatc gcctcgcatg tggtgtatat
    19201 ctgggccgat gccaacttct atcgcccgat caccatgcag cgcgctaacc tgccgctttc
    19261 gtacccgatg acggcgcgac gtttccttga gaagcatggc ctgcttgatg cgcaggagta
    19321 tcaacgccgt cttattgagc aaggtaatcc agacgccgtt tccgtacagt atccgttaag
    19381 cgaactgcgc tatcgcgata tgggcaccgg gcagaatgtg ctgttgatta ctgtcgatgg
    19441 cctgaactac tcacgcttcg agaagcagat gcctgcgctg gcaggttttg ctgagcaaaa
    19501 tatttcgttc acgcgccata tgagctccgg caacactaca gacaacggca tctttggcct
    19561 gttctatggc atctcgccga gctatatgga cggcattctg tcgacccgta cgcctgcggc
    19621 attaattact gcgcttaatc agcaaggcta tcagctgggg ttattctcat cagatggctt
    19681 taccagcccg ctgtatcgcc aggcattgtt gtcagatttc tcgatgccga gcgtacgcac
    19741 ccaatctgac gatcagaccg ccacgcagtg gatcaactgg ctgggccgct acgcacaaga
    19801 agataaccgc tggttctcgt gggtttcttt caatggcact aacattgacg acagcaatca
    19861 gcaggcattt gcacggaaat atagccgggc ggcaggcaat gtcgatgacc agatcaaacg
    19921 cgtgctcaat gcactgcgtg attctggcaa actggacaat acggtggtga ttatcactgc
    19981 cggtcggggt attccactga gcgaagagga agaaaccttt gactggtccc acggtcatct
    20041 gcaggtgcca ttagtgattc actggccagg cacgccggcg cagcgtatta atgctctgac
    20101 tgatcatacc gatctgatga cgacgctgat gcaacgcctg ctgcatgtca gcacacctgc
    20161 cagcgaatat tcgcaaggtc aggatttgtt caaccctcaa cgccgtcatt actgggttac
    20221 tgctgcggat aacgatacgc tggcaattac caccccgaaa aagacgctgg tgctgaacaa
    20281 taacggtaaa taccgcactt acaacttacg tggtgaaaga gtgaaagatg aaaaaccaca
    20341 gttaagtttg ttattgcaag tgctgacaga cgagaagcgt tttatcgcta actgattaat
    20401 tataaatcag ttagcgaaat atcttacttg caatcggtgt ggaaaacggt agtattagca
    20461 gccacgagtc ggcacgtagc gcagcctggt agcgcaccgt catggggtgt cgggggtcgg
    20521 aggttcaaat cctctcgtgc cgaccaaaaa tcccaagaaa aaaccaaccc ttacggttgg
    20581 tttttttata tctgcaatta attcgataaa cagaccgtga cacatcacag cctgtttatt
    20641 ttctgttatc agaacgtcca gaccacaccc gcctgagtat tccacggcga ttctacccct
    20701 gcgccattgc catagctgac tgacaaatga ccgcttaacg tcggggtaaa cgatgacctt
    20761 atcccagcct gataaacgcc actggttcca gccacatcat tattaaattt gccatcgtca
    20821 ttaactttca cctggttaga atcggcgtat tcctgacgca cggcggcttt cagccagggt
    20881 tccagcgtcg taccgttttg caggtccatg tgatagctta ccgccgttcc cgcttcagcg
    20941 cgtaatatcc gggtatttcc cacatccgcg cgcatgccgt ttgataacgt gtagtcctga
    21001 ccatctgtgg taaagccggt aaaggccaga tagggtctaa cactccacaa tccgtcaacc
    21061 caacggaacc cgctctcaac atgagcaccc gcgccgttac tattgtaatc gccaaacgct
    21121 gttgccccat tactcatctt gccatggatg gtgttggcaa aacggtcaac tttcaccacc
    21181 ccatcaacat aggcaccgtt ctgatgctcc caaccggcat aagcccccag ggtatagcta
    21241 tcgatattac cttttccgcc gcgatcaaaa ccaatatcag aatgagagta accaaagaac
    21301 aagccgcgaa ttgtactgct ttcttcacgg gagaaacggc tatcgatacc gagcgtcagg
    21361 cccgtcaatg tttgctcaaa accagctccc gcatcagtgg tcacgttgtt gcgggtgtta
    21421 attgccgaac tccacatcac cgtatcgtaa ctaacgcctt ttacgctacc aagacgctca
    21481 cgcacggtgt ccagttctgc atcaaatacc agcggttgtg cggctgccat gttcagcaca
    21541 tcagtggttg aaggggtaat ttgcgcgcga ttctctgcga ggctccagct atggttgcca
    21601 ttatccagca aggtatattc atacgtaccg atatcaacaa cgcctccggc attgcccaac
    21661 gtaaatgcag catcaccgcc gcccgttgtt accagtgtaa ggctatctcc tgctgccggg
    21721 ctggcaccgg tgtccgtcac gaatattttg aaatcacctg ttgcctgacc ggtgacgttg
    21781 agctgatcgc tctgatgatt ggccatatcg gtacgcatat aaaaattgcc gtttcccgac
    21841 agggtattgg tggtcaacgt aatgaaattt tccgcacttg ccgtcacaga tgaacgagtc
    21901 actggctcag ccatcataat cacatttgcg ccggacatcg caaggttggc taagttgagg
    21961 cgagtgtttt ggttcgtcgt agtgatatcg gcaatgatgg tccatgcgct attttcgagc
    22021 gaaacgtccg ctttgctggt atccacggca ccatgcgttc taaaagaagc gccctcagaa
    22081 accactacct tacccaccaa catagcgggt gcataattta tttgcggctt catgacctca
    22141 agaatgccat cattccctct gactgacacg ttaaccattg tgccagccca gacattagcg
    22201 cggccattgt tgattaccat attttcagag atggcttttg aggaatacgt gatacttcca
    22261 ttctggtctg atcgaccgag atcatatacc gcatttgcct gcactttcgt accagtatcc
    22321 tttcccagcg attgcatagt ggcatgctca tcaaggatag tgtcaatggc ctgcgtgtcc
    22381 tccataacaa tgagcccgga accatcatcc agttcataat tttttgacac accatccttt
    22441 atactaaatt ggcctttact gtttgtacca ctcacttcca gcgcattcgt tgagacaatt
    22501 aatttcccgc ctgctttttt atcaacgcca gttgcagtcc cgccatccat aacctccagt
    22561 aagccgccac tatccacagt agtattataa gcgaagtcat tttcttcaac tcgcaaactt
    22621 ccgccgtttt ccaacaacat attattagca ataccattct tgatatcgaa ctgaccgaga
    22681 cggtttgttc cgaatacctc catggcccgc gtggttgttt taatagcacc gcctgttttc
    22741 tgatctaccg caagcgcaaa tcccccctct ttaacctcca gtagtcctcc cttttcgata
    22801 gcggcaaaaa cggccccccc atctttttca acgattaagc gcccgccttt aatcgtggta
    22861 tcattcgctg ggccataaac ctgtagcaca ccattattga gggtagtctg gtttgccgcg
    22921 ccacccattt gaacaatttg ataaccatca tttaatactg agtattctgc ggtaccattc
    22981 atttcaatgt attgataccc tccgttaatt tcagtattac tacttacacc aaattctttt
    23041 atatgctgtt ctccgccagg attgattttc gtattattgg cggtcccata aaccagctgg
    23101 atatcttttt ccaggataac accatcaact gtctcaccat actcaactgt tgatgccaat
    23161 gctatgggtg aatataacgc tgtagccacc agaacagata ttgcggaacg acaaagagaa
    23221 acagaaccag attgatgcat tgagctttca tcctatgaaa ttaattgcag ttaaaagcat
    23281 tgggtacaga aaatacccat agctccatac ccggagtcag tttttaaaaa ctgtttaaag
    23341 aaatgcacaa gtattgtgat tgatttttta gttgtttttc ttgatgagaa gctgatgcaa
    23401 aattccgtct ttataataaa aatgatgcca aagcgaacga caaggttgta gttttcacta
    23461 catgtccata cataaaatgg ggtaacattc acgcgcctgg tagcgttacc aacgctacgc
    23521 tcaaacataa tgattctaat aaaacctcag gagactacta tgcctgaagc aacgcctttt
    23581 caggtgatga ttgtggatga tcatccactt atgcgacgcg gtgttcgtca gttactggag
    23641 cttgatcctg gctttgaagt ggtcgccgaa gcgggcgaag gcgcgagcgc gatcgatctg
    23701 gcgaatagac tggatatcga cgtgatcttg ctggatctca atatgaaagg tatgagtggc
    23761 ctggataccc tcaatgcctt gcgcagggat ggcgttaccg cgcaaattat tatcctgacc
    23821 gtatccgatg cctccagcga tgtctttgcg ctgatagacg caggcgcaga cggttatctg
    23881 ttgaaagaca gcgacccgga agtattgctg gaagcgattc gtaccggagc gaaaggcagc
    23941 aaagtcttta gcgaacgcgt caatcagtac ttacgtgaac gtgaaatgtt tggcgcggaa
    24001 gaagatccct tcagcgtgct gacggagcgc gagctggatg ttctgcacga gctggcacag
    24061 gggctgtcaa ataaacagat tgcatcggtg ttgaatattt ccgagcagac agtaaaagta
    24121 catattcgca atctgctgcg taaactcaat gtccgctcac gcgtggcggc caccattctg
    24181 ttcctgcaac aacgcggggc acaataaaaa tagcccgatg gatttatcat cgggctgaga
    24241 tttatgacaa acgcaaaact gcctgatgcg ctacgcttat caggcctacg tggatcgatc
    24301 aatttattga atttacacaa ttttgtaggc cggataaggc gttcacgccg catccggcat
    24361 aaactaagcg cactttgtca tcagtctagc ccgatggcat caccatcggg cctcttttta
    24421 tttactctcc tgcggcgaca aatgttgcat cgcctgcgcg atactacgtt caatcaccgc
    24481 acggcgagta tcgttggcag gtaagagttt caacatcatc tcccacgcgg caacggcttc
    24541 gccaaatcgc tgctgctcaa aggcattaaa cgcatacatg cttagcacac ggatattgct
    24601 atggtccgtt ctcaccagct gacgtagcag ttcaccgccg aggcggttgt cgttgggatc
    24661 agatgaacga gtcaacgctt cagcgtatcc cagtgcggca tcgctgttct tcggatcgag
    24721 gcgatacgca gtagcgtatg catcggtggc gatactggcg ttacccagcg ccatgccaac
    24781 gcggcccaac ataatccagc cttctatatc tcccggattt ttttgcagtt gagtacgcat
    24841 ccccagcgca aggcgcgaca tctcttcttc gttgagcgga tcggctttcg ggtccagcgc
    24901 cctgtccagc aacgccggag cctgtgccgt ggcctgctgc cagattttca cctgctgata
    24961 attgccagtc tggtagtagc tgacgccagc cacaattaac gccaccacaa tacccggcag
    25021 ataaacaaca tatccggcac gcttaccttc cggcacgctt tgttcaggaa acgcttccgg
    25081 caccacgcgt acccgacgcc gcgagcgggc gtatatgacc caaccgccaa tgccaatagc
    25141 cactactggc agcacccaca gcagcacggt cagcggcgtt aacggcggat cgtaagtgac
    25201 gaagttgccg taacgcgcca ccatataatc gacaatctct tttttacttt taccttcctg
    25261 catcagttca tacactttct gacgcaggtc ggtggcaatc atcgagttgg aatcggcaat
    25321 gctgttgttc tggcatttcg ggcagcgcag ttcttcagtg agctgacgga actgttgttc
    25381 ctgtgcttca tctttaaact gcaacacgtc gatggtcgcc agcgctgagc cggagatcat
    25441 cagcatcagc acgcccaata aaaacctcat tgtgcggcct ccttactgta tttctcccac
    25501 agcggcttga tctcttcttc ccagacgcga ggattgagat cgcccgcatg gcgatagcga
    25561 atgataccgt tgccgtcaat aaggaaggtt tctggcgcgc catagacacc gagatccagc
    25621 cctaacatgc cgtcgccatc aaacaggctt agcgcgtaag gattgcccag ctctttcagc
    25681 cagctgattg ccttctggcg atcgtcttta tagttcatgc cgaccacgcg aatgccctgc
    25741 gctgaaagct gattcagata ttgatgttcc gcacggcagg tcggacacca agtcgcccag
    25801 acgttaagca gtactggttt gccctgagtc agcacatccg cctgataaaa ctgccccgga
    25861 ttgtccagtg attcgagacg aaacttcggc acaggcttgc caatgagcgc cgattccaga
    25921 ttggtcggat catccccttc ggcattacgc gccagctgcc acagcagcgc cgcggcaatc
    25981 gccaggaaga taatcaacgg aattaacaat actttgcgct tcatacggcc tccggcgcag
    26041 ttttttgcgg actcacgcgc ttacgatagc gaggatcaaa cagacacagc agtccgccca
    26101 acgccatcat cagcccgccc gcccaaatcc agcgaacaaa tggtttgtag taaagacgca
    26161 cggcccacgc gccgttttcc agctcttcac cgagcgcggc gtacaggtca cgcgtgatgc
    26221 cgccgtcaat tgccgcttcg gtcatcatcg acccggcagt gttgtaataa cgtttttccg
    26281 catacagcac cgtttccggt ttaccctctc gcgttacgcc aatagtcgcc acaccgccgc
    26341 gccagttcgg gccagtcacc tctttgacat cacggaaggt gaagcgatat tcatgaatat
    26401 cgacgctatc gccggacttc atgcgcacat cacgctcaac gctataattc tggctaaagg
    26461 caatgccaac aattgtcact gccagcccca ggtgagccgc caccatcccc caataactga
    26521 aggtggtttt cgtgccgcgt gaaatacgca gcgcagcttc cgcagttgcc agcaccgcaa
    26581 tccagcaggc cattgccagg ccgagcaccg tcatcgccac aactttgctt tcgaacagcc
    26641 acggcaacag cagagacagc accagcgtag agatgaaggc gataatcaat aaattgcgga
    26701 ttttacgtgg gcgatcgcgc ccccagcgca ccagaggacc gacaccaagc agtagcgcaa
    26761 acggcaccat cagccaggta aacatggtgt tgaagaacgg ttcgccaatc gaaatactgc
    26821 ccagtccaag ttgcttgtgc accagcggca gcagcgtccc cagcaacacc accagcatcg
    26881 cggcgaccag caaaacgttg ttcgctaaca gcaaagattc ccgcgaccac agcgcattgt
    26941 ttacgcgtga gcgaactttg tgtccacgcg cggcaaacag cagcagcgaa ccgccaatca
    27001 ccagcaccat aaaggcgagg ataaacatac cgcgcgccgg atcagacgca aacgcgtgta
    27061 ccgataccag cacgccggaa cgcacgagga aagtccccaa cagacacaac gagaaggcac
    27121 tgattgccag cagtaatgtc cacgctttga agctggcgcg ttgttcagtg accgccagtg
    27181 agtgcatcag tgcagtcccc accagccacg gcataaacga ggcgttttct accggatccc
    27241 agaaccacca gccaccccag ccgagttcgt aataggccca tgcggaaccg agcacaatgc
    27301 cgagcgttag gaaaatccac gccgccagcg tccacgggcg agtaaaacgc gcataagtgc
    27361 tgtccagacg cccgctcagc aaagaagcaa tggcaaaagc aaacgccacc gagaaaccca
    27421 cgtaccccat ataaagcaga ggcggatgga agatcagccc cggatcctgt aacagcgggt
    27481 taagatcgcg cccttcaatc gggaagttcg gcagcgtgcg tgagaacggg ttagaggtaa
    27541 agagaatgaa cagcaaaaag ccgacgctga ccatccccat tatcgccagt acacgggcca
    27601 caatatccag cggaatacgc tgactaaaaa tcgccaccgc aaaggtccag ccgctcatca
    27661 gcagcaccca cagcagaagc gaaccttcat gcgcgcccca agtagccgcc acgcgatacc
    27721 acaccggaag ctgggtattg gagttgctgg caacataggt gacggtaaag tcgttgacca
    27781 cgaaagcatt gaccagcacc agaaatgcgc cagccacaga cataaacagc agccaggcaa
    27841 acaagcggga agacgccatc atgcgcgcat ctccgcgcgc cacgccccat agcggataca
    27901 cggacagcag cagcgcaatc cctaacgcca agcacagcag cccgtttcca atttccggca
    27961 tcatgatgct gggtccttat aaacactcgc cgggcgacgg tggttagctt ccatcgcttt
    28021 ctcaacttct ggcggcgtgt agttttcgtc gtgtttcgcc agcacttctt tcgcgaggat
    28081 atgattgcct ttttccagct cgccctgcac cacaacgccc tgcccttcac ggaacagatc
    28141 cggcaaaatg ccttcgtaag agacatccac tgagccttca gcatcgtaaa tggtgaaggt
    28201 cactttcagc gaattgggat cgcgctgcac gctacccggc atcaccatcc cgccaacgcg
    28261 cagacgctga ccgacttccg gcatctgctg agtttcacgc ttgccgtaga gaatttcccc
    28321 cggcgtataa aagagatcga tattcgagcg cagcgcatat agcaccagac cgatagtcag
    28381 cgctagccct gccaacacgg cacaggcaat ccacaagcgg tttttacggc gaatattcat
    28441 gcagcctcct gctgttgcgc agcacgtaaa cgcgcctcac gcgcccgctg ttgcgccaca
    28501 ccacgcagaa ttgcgcgatg ttgcatcacc gagtgcacga ccaaaaccac cagcggaata
    28561 acggtcatca ccaccgccag ccagacaaaa aaggcgtaac cgcccattgc gaaaaattca
    28621 ttccaggaag caaatgcagg ggtcatttac ggcctctttt cagtatcagt tcactcaccc
    28681 acggacggcg tttttccatc agcaaaatca aattacgcat ccgcatcagc gtcagcgtgg
    28741 cagacaggag caggaagcca aaaatcgacc agcgcagcgg cgaacgcatc gccggatcga
    28801 tactttgctg catccgcgtt gatccctgat gcagggtgtt ccaccactcc acggagtaat
    28861 gaataatcgg cagattcacc acgccaatca gcaccatgat acctgccgca cggcccgcca
    28921 gacggcggtc gtcgaaggcg tgccacaggg caatcacacc cacatacaaa aacagcagca
    28981 ccagttcaga agtcagacgt gcatcccata cccaccaggt gccccacatc ggttttcccc
    29041 atgcagagcc ggtaaccagg gcaataaagg taaacacggc accaatgggg gccatcgccg
    29101 ccaccgccag gttggccatt ttcatctgcc agacaaggcc aataaacgcc gccaccgcca
    29161 ttgatgcata aatgcccatc gaccagatcg ccgcaggcac atgcaggtag ataatgcggt
    29221 agctatttcc ctgctgataa tcagccggag caaagccgaa tccccagatc cagccgacgg
    29281 taagcacgac cacactggca attgccagcc acggtataaa ccagccacag atttgataca
    29341 gccgtggtgg gatcgccagt tgatgcagtg ttttccacat agtttcgata ccagactcga
    29401 acaaaaatca gtaatccagc gttattgaat gctgattcgt aacgctgccg ccgtcgcaaa
    29461 aggacttaat gtcgcggtgc ctgccagcaa cgcgcctaaa attgccagat acccatcaac
    29521 gggcaaatgc atagaagccg cgtccatcgc ggcggtggca aagatgagta atgggatagt
    29581 cagcggtaac accagtatgc tgagcagcac accaccgcgc ttaagtccca ctgtcagcgc
    29641 cacgcccggt gcaccgagaa agccaagcgt aggcgttccc agcagcagcg tcagcgccat
    29701 cacttgccag ccataaacat ccattcccag tagcattgct accagtggcg aaaggatgag
    29761 taacggcaga ccggttacca tccagtgcgc catcaccttc gccagcacaa cggcgggcaa
    29821 gggtaacggc aacaacatca attgttcaag actgccgtcc tgcaaatcgt cacggaacag
    29881 tcgttccagc gccagcaagg atgaaagcag cgcagcaacc cagataatgc ccggtgcaat
    29941 acgcgccagc agttgcggct ccggaccgat actgagcgga aaaagggtaa ttacaatcag
    30001 gaagaaccac agcgggttgg cgatttcggc gctatggcga aacgctacgc gcagttcaag
    30061 acggaaaata cgccagaaca tcatgcggcc ctcgtttgcg tcagtgaaat gcggcgaatt
    30121 ttactttcag caacgttgag cggctggtgg gtagtcagaa tcacaatccc cccctgctcc
    30181 gtatgctgcg ccatacgctg ggtcagacga tcgacgccgt taacgtcaat cgcggtaaaa
    30241 ggctcgtcga ggatccataa tgtggcacgg gtcaaccaca gacgcgctaa agcgacgcgg
    30301 cgttgttgcc cggcagaaag ttgatttacc ggaatatctt cgaatccggc aagcccggcc
    30361 tgcgccaagg cttccagaca ttgtgcggca tcgccatcgc gatgataaaa gtgcagattt
    30421 tctaacgccg tcagccgggt tttgatcccc ggctgatggc ctatccataa cagggtttga
    30481 tggtagctgt cgcgtacctg atgcaagggc tgcccttgcc agagaacctc gccagcgtca
    30541 gggcgagaca accccgtcag caaacggaga agcgttgtct tccccgcgcc gttgctaccg
    30601 gtgatttgta cccactctcc tgcgttcagc gtaaatgaca agccactaaa taaggttcgt
    30661 tcatcccgct cacaaagtaa ctctctggct tcaagcatac ccacgcaata accctgttaa
    30721 aaacctggct cgacttcacg catatcgggc agcttgtgcg cgatcccttt atggcaatca
    30781 atacaggttt gcccatcttt caccgcctgg tcatgcatct tcgcggcaac cgatttctgg
    30841 gcggttgtat ccatatactc gaagttgtga cagttacggc actcctgcga gttattgtcc
    30901 ttcatgcgcc gccactcatt ctgtgccatc gtcagacgat gagcttcaaa tttctgcggc
    30961 gtgtcaataa cgccaaaaat tttaccatac agctctttac ttgctttgag cttgcgtatc
    31021 atcttcggca caaactcgtg cggaacgtga caatccggac aggtcgcacg gacgccgcta
    31081 cggttgttgt agtgcacgga atccatgtat tcctgataca ccgtgttgcg catttcgtgg
    31141 cagctaatgc agaactcttc ggtattggct ttttccatcc cggtgttaaa gcccccccag
    31201 aagacgatgc cgccaacaaa accgatcaac agcagcgtcc ccagcgccag atggctgggg
    31261 gtacgccacc atttccacag gcgcttaatc agaccaggct tacggtcaga atttcccata
    31321 ataacctctt atttcccgta accttttgat ggggtaaagg tattccccac gattggcgcg
    31381 gtatcggcct gcggtacgtg acattgcaga cagaaataac gacgcggagc cacttccgcg
    31441 cccactttgc cgtcgctgtc cataaagtga gtaggactga tacgcggcgc gccagtggtg
    31501 cgatagcttt cgacaccgtg gcactgcaag cagcgattgg tattggtcgt tacctgataa
    31561 ccttcaacgc tatgcgggat cattggcggc tgattcacat agttcagcgg catccgatcc
    31621 tgctcttttg gcatccgaat ggccccttcc tgcgtcccgg atacttccgg cgactggcta
    31681 aaatcgacgc cattagccgc ccaaaccgcg ccgcttacca ccagggccag catcgccgtc
    31741 cattgacaca gcgctttctt caggtcatgg cttttcatga tttcgctccc gaactccatc
    31801 gtgtagttat tgtaaataca tcctcagaac agacatccac gcagcgaccg cacgtcatgc
    31861 aatcgcggct ggtgacctgc accgggcttt gttcatccag caccggggca cgtagcacat
    31921 gcggttccgg gcaaacatga aaacaatcca tacagcggtt acatttctgc cgatcgctgg
    31981 ccgcaacggt aatcacacct ttgctaccca gcacgccata cagcgcgcca accggacaaa
    32041 tgtgcccgca ccagccgtgt tcaacgacca gtagatcaaa taaaaacagc gcgagaataa
    32101 gcagcgcgcc gctgccgaag cccattacca ggctacgccc catcagagaa acggggttga
    32161 tccattccca aatgagcgtg ccagttaagg ctgatcccac cagaatgacc accaacagca
    32221 cgtagcgaat gtggcgaggg atcgtcgcag actgattgag gtcaaagcgc ctgcgtaacc
    32281 agttcgctaa gtcggtaatc gggttcagcg ggcagaccca gctgcaaaat aatcgctttc
    32341 ccgccagggc gtagagcacg gtgataatca ccgcaccggt caacgccacc gtggcaggca
    32401 gatgaccact ggcaagactt tgcagcgtca tcagcggatc ggtcagcgga acggtgtcga
    32461 ataacaggct actgctgtag ttgccgtgca agatccacac accaaaccat ggaccgctca
    32521 aaaacatccc cagcacgaag aactgacaaa ggcgacgtaa caccagccaa cggtgactgc
    32581 gccaccagcc ttttttctcc agcgcctcgc gcccggcgtc acgtttacga tttgccattg
    32641 ttcccctcca gccagccgaa gcggtaatgg tgacctaact cccctttcgc cagtgacagc
    32701 ggtaacacct tgattgccgg ttgttccagc acgcatactt tttcgcactt accgcaaccg
    32761 gtacaggcgt cgctgtgaac cgtcggcaga aagcgggcgt gcttaccggt acgcgtgttg
    32821 cgctccagct ccaaggtgat ggcctcatca attttcgggc attcgcgata acaaacatcg
    32881 cagcgcagcc cctgaaagtt gagacagttt tcctggtcca ccagtaccgc cagccccatc
    32941 cgcgcgtcgt cgatcgattc aatctcacga tccagcgcac cgcttgggca cactttggcg
    33001 cacggaatgt cctcacacat ttcgcaagga atatcccgtg cgacaaaata tggcgtgccc
    33061 gccgacagac cagaggccag cgtcgccagt tttaaggtgt cgtaagggca agcctgaaca
    33121 cactgaccgc aacgcacaca ggcactggca aaggcgttct cgtttatggc tccgggcggg
    33181 cgcaaccgca cgccagatgc gcgtgcggtt tgctgttgta accccagcgc cacacccacg
    33241 gcagccagcc cgcctgctgt gcgaacgaca tcgcgcagaa agcggcggcg accattttga
    33301 ggtttcgctg accgggacat aatggcttac accttctcca gtttgaccgc gcacttcttg
    33361 aagtccgtct ctttcgagag cggatcggtc gcatccagcg tcagtttgtt aaccagctgt
    33421 gcggcgtcga agaacggcat gtacaccagg ccctgtggcg gacggttacg accgcgcgtt
    33481 tcaacaatcg agatcacttc gccacggcga gaaaccactt tcactttgtc gccacggcgc
    33541 agatcgcgcg ctttcgcatc cagcgggtga ataaacaaga ccgcttccgg gaaggcgcgg
    33601 tgcagttccg gcacacggcg agtcatactg ccggtgtgcc agtgctccag aacgcgtccg
    33661 gtagagagcc acaggtcgta ctcttcatcc ggtgcttccg ccgccggttc gaacggcagt
    33721 gcgaagatca ccgctttgcc atccggttta ccgtagaact tatagccttc gcccgctttc
    33781 acgtacgggt cgttaccttc gctgtaacgc cactgtgttt ctttaccatt caccaccggc
    33841 cagcgcagac cgcgcgcttt gtggtagtca tcgaacggtg ccagatcgtg accgtgaccg
    33901 cgaccaaacc atgcgtactc ttcgaacagc cctttttgca gatagaagcc cagctcgcgg
    33961 gattcatcgt tcagctgatc ttccgccagt tcggataccg ggaatttgct cacttcgggg
    34021 gtggcataca gaacttcgta cagcgttttg ccacgcagtt ccggtttctt cgccagcagc
    34081 tcttccggcc atacttcttc agttttgaag cggcgggaga actggactaa ctgccagaga
    34141 tccgatttcg cttcgcctgg cgcctgtacc tgctgacgcc agaactgagt acggcgttcg
    34201 gcgttaccgt aagcgccctc tttctctacc cacattgcgg tcggcaggat caagtcggcg
    34261 gccagcgcac tgactgtcgg atacggatcg gagacgatga tgaagttgcg cggatcgcgc
    34321 cagcccggca tacgctcttc attgatgttc ggcccggcct gcatgttgtt ggtacacatg
    34381 gtccagtaaa cattcagctt gccgtctttc agcgcacggt cttgtgccac cgcatgaaga
    34441 ccgattttcg ccggaatggt gccgctcggg atattccact tcttctcaca gatatcacga
    34501 tgtttctcgt tagttaccac catgtccgca ggcagacggt gagcaaaggt gcccacttca
    34561 cgcgcagtac cacacgcaga aggttgcccg gtcagggaga acggaccgca acccggctgg
    34621 gaaattttgc cggtcagcag gtgcaggttg tagaccaggt tgttagccca cacaccacgg
    34681 gtatgctggt tgaagcccat cgtccagtag gagatgactt tcttgttcgg atcggcatac
    34741 agctgcgcca gttgttctaa ctggtctttc ggcacgccgg tcatttcagc ggttttttcc
    34801 agcgtatact cagcaacgaa ggctttgtaa tcttcaaagc tcatcggttc ggaggcgtca
    34861 gaacccggat tcttcgctgc tttttccagc ggatgggtcg gacgtaaacc gtagccgatg
    34921 tccgtcgccc ctttgcgcag gttaacgtgc ttgctgaaga agtcctgatt tatcgcattg
    34981 ttttgaatga tatagttggc gatgtagttc aggatcacca ggtcagattg cggcgtaaag
    35041 atgatgccgt tatccgccag ctcgaagcta cgatgctggt aggtagaaag caccgccacg
    35101 gtgacgttct ggttagagag acgactgtta gtgatgcgtg accagaggat cgggtgcatc
    35161 tccgccatgt tagcgcccca cagcacaaac gcgtcagcct gctcgatgtc gtcatagcag
    35221 cccatcggct catccatacc aaaggtacgc ataaagccaa ctactgccga cgccatacag
    35281 tgacgtgcgt tcgggtcgat gttgttcgaa cggaagcccg ctttgaacag cttggacgcg
    35341 gcataacctt cccagatagt ccactgacca gaaccgaaca taccgatcga ttccggccct
    35401 ttttctttca gggcggtttt gaatttctct tccatcacat cgaaggcctg atcccaggtg
    35461 attggggtaa attcgccttc tttgtcatat ttaccgtttt tcatacgcag cagcggctgc
    35521 gtcaaacggt ctttaccgta catgatcttg ggcaggaaat agcccttaat gcagttcagg
    35581 ccacggttaa ccggtgcgtc cgggtcgccc tgacaggcca ccacacgccc ctgctgcgtt
    35641 ccgaccagaa cgccgcaacc agtaccgcag aaacggcacg gcgctttatc ccatttaatg
    35701 gcttcctgct gaccaacaac ggcgcgggca acgcccggca cgctgagacc ggcagccgcc
    35761 gcagcggccg caacggcgtt agctttcata aagctacgac gactgagttt catggtgttt
    35821 cctcaccttg ctcttcctgc tggtgataaa ccagcgacac cgccagcacg ccctctacgt
    35881 tgcgtactga ctcaatggtt tggatcagcg tttcgctgtc ttctgcttcc accaccacaa
    35941 tcaactgacc gctcggcgcg tcgctgacag caacttcaca gccgggaaag gcgttcagtt
    36001 gggtgctgat gtctgaaatt cgttcgcttt tggcctgcac gaccaggctg caaacttgcc
    36061 agttagtgtg catggagata ctccgcagtt atggctgata ccggacagct ggctgcacat
    36121 gctccacatc cgttgcaaag ttggctatta agttgcgcct ggtagatccc ggagagcgtc
    36181 gggcgaaaga taattgccat tggctcacag ctatcctgac agcggcggca ttcaactgac
    36241 tgatacgcca ggcacgcgtc cccgatggtg aactgcaaat cccaggccct ggtgtggcgc
    36301 ggagaaaata gcgattcggg gcaagcctgc gcgcaggcgt aacagaagct gcactcgtta
    36361 tttttgaaat taacgctcgg atagccaccc gtgccgcgtt gcagaatgtt gttttcacaa
    36421 gcattaatac aggcgtcgca gcgggtgcaa tgggtcagaa aatgagattc atcaccggac
    36481 cagggcggac ggataccgtt actggctttg cgccagcaac cagtgagtat gccccgacgg
    36541 gatgcatcaa tcttcacatt gaccttccat cattaacgcg ctctgaaaat tgagagcgac
    36601 caaatagacc gcataattaa taagccattt ttatagccgc taagatatta aaggatgtgt
    36661 caaagatgca taccccgatc ggggtaaaac ctgtagcagg atcaaaagag tggcgggaag
    36721 cgtggcaaaa acgggctttt gctcacattt caaatggtta taaacatatt tatatagcga
    36781 ttaattcacc agagatattt ctgctggttt gctccctcat cagaatttaa cactaaaaga
    36841 gcaggtaaaa ttgtctgaat gttctttaag ttattcataa agcaaattaa taaatctgat
    36901 gaatatgtta accttcagcg acatcatcgg tgaaaaccta taaatgaaga aggaaagcaa
    36961 aaaaatgaag accattctac ctgaagtatt gtttgccgct ttcgctacca cttccgcctg
    37021 ggcggcagaa agcgtccagc cactggaaaa aatcgcgcct tatccacaag ctgaaacagg
    37081 catgaagcgt caggtgattc agttaacccc gcaagaagat gaatctaccc tgaaagtaga
    37141 actgttaatc ggtcagacgc tggaagtcga ttgcaatttg catcgtctcg gcgggaagct
    37201 ggaaagcaaa acgctggaag gctggggcta tgattattat gtctttgata aagttagctc
    37261 gccggtttcc acgatgatgg cctgcccgga tggcaagaaa gagaagaaat ttgtcaccgc
    37321 gtatctgggc gatgctggaa tgctgcgtta caacagcaag ctgccgatcg tggtgtatac
    37381 gccagacaat gtagatgtga aataccgcgt ctggaaggcg gaagagaaaa ttgacaacgc
    37441 ggaagttcgc taaactgttg tgaagtacgg caccccgttg gttggataag gcggttacgc
    37501 cgcatccaac atctaacgcc cgagccggtt gcctgatgcg acgctggcgc gtcttatcag
    37561 tatacgccac atcattacaa cgcaatatcc gccacttctt tttgaacggg ttgcggtttc
    37621 aactgcggtt tcggcgtact atctgctgct tgcggtttgt cgtatttcag ccccagcact
    37681 tcgctggtgt actgcaactc gcgttctgtt gccgctacat caccgttcag cttgcgtcca
    37741 taagacggaa cgatcgcttt caacgtagcc tgccattgcg ggctggaaac gcgatcgcca
    37801 aatacttttt ccagtagatt caacataatc ggcgcggcgg ttgacgcccc tggcgatgcc
    37861 cccagcagcg ccgcaatggt cccttgctgg tcactgacca cttcggtacc cagacgcagt
    37921 acgccacctt tctcggcatc acgcttgata atctgcacac gctgccccgc ttgccacaaa
    37981 cgccagtcct cttttttcgc ttgcggatag tactctttca acgcttcaaa acgatcctct
    38041 tcactcaaca tcacctgact caccagatat ttcaccagat cgaaattatc cagcccgacg
    38101 tgcatcatcg gcatcacgtt agaggtggtg gtggaactca tcagatccca caatgaaccg
    38161 tttttgagga atttggttga gaaggtggca aatggcccaa acagcactac gcgtttaccg
    38221 tccagaacac gggtatcgat atgcggaacc gacatcggtg gtgcgccaac ggatgcttta
    38281 ccgtaaactt tcgccagatg gcgattaacc acgtctggat tttccgaaac aaggaactgt
    38341 ccgcccaccg ggaaaccggc gtagtctttc gcttccggaa tccccgattc ctgtaacagc
    38401 ttcagcgccg caccgcccgc gccgataaag acaaatttgg cgcggatgtt ctgtgcagtg
    38461 ccgtttttca gatcggcaac ggtaacggtc caggtgttat cgtcattacg ctttagggcg
    38521 cggacttcgc tgctgagttg cagcgagaag ttagatttct tctgtaagga agcaattaac
    38581 tggcgggtga tctcaccata gttcacatca gtgccaattt ctgttgcgga tcgcgccctt
    38641 ccatcactaa cggtgcccac tctttgatct gcgcgtgatc ttcagagtaa cgcataccgc
    38701 gaaacagcga gctttgttgc aacgcggcgt aacgggcgcg caggaaatta acgttatcct
    38761 cgccccagac aaagctcata tgcggaacgg tattgataaa tgaacgcgga gtacgcagca
    38821 cgccgcgctc gacctggtgc gcccagaact ggcgggaaat ctgaaatgct tcgttaatgg
    38881 cgactgcttt ttcaatactg atgctgccat cggcgttttg cggggtgtag ttcagttcca
    38941 tcagtgcaga atgcccggtt ccggcgttgt tctagccgtt cgaactctcc tgcgcgcccc
    39001 cctccaggcg ctccaccatg gtcatcgacc attcaggctc cagctcgcgt caataggtcc
    39061 ccaacgtggc gctcataatg ccgccgccaa tcaacagtac atcagtttcc tgctcctcgg
    39121 acgctttcgc ttttgccgcc atcgaaacgg cattaagccc cacggccatc gagaagagca
    39181 tggcagtcac ttttttcatc ttgttaatgc cttactttta gtcgctttat tgcaggtgag
    39241 atttgcgcgg catcaacggt aacacttaag taacaacatt taaataatgt ttataaatta
    39301 tattctaatt ttagaaacat tataatttcg ttgattaatt atagggttat tagaaatgaa
    39361 gggattttta gcatattgac ctgacggcag cagtccgcca ggtcagcgtc gtaatattat
    39421 gccgtccggg caacggcatc acgcgaagcg gcatcgcgct cttcgcccgt cagctcgcta
    39481 agttgcccat tgcgcatttc cagcaggcgg tcggcgtgga taaagtaatg atcatcatga
    39541 ctgatagcga aaatagtttt acccatctcc tgcatcagcg gcagcaacac ctgataaaac
    39601 tcacgacgga agtgtggatc ctgatccacc gcccattcat ccagcaggat aatatcgcgt
    39661 tcttctgcca gcgccagcaa cagcgccacg cgttttttct gcccttttga taacttcagg
    39721 ttaacaatac gcccgttgct taactcaagc ttatgagcca ttttcagctg cgccagccac
    39781 ttctcaacca gttgcgggtt agcgggttgc ccctccggcc ccagcagttg atcaaacagc
    39841 cagacatcgg taaacactgc cgaaaacagt ttgcgataat cttccggttg ttcgccgctg
    39901 acaggtttgc catccagcaa gatttcgccg ctttgtggct gatacaagcc cgtcaacaac
    39961 atcgccagcg tcgattttcc gctaccgtta ccgccaatca gaaacagcag ctcgccacgt
    40021 ttgatggtga gattaatcgg accaacggaa aacgcgttat cctgataagc aaacgtcacg
    40081 ttacgcagct ccagcgtttg ccagttggga aacgcctgcg ggcgcggaaa ctcagctttg
    40141 aaaggcgcga gcgcgaattt gttcagcttg ttaaacgcca cctgcgccgt cagcagcgtc
    40201 ggcaatgcgc caaccgccga aagcagcggc gtacgcagga ataacagcgt caacgaatag
    40261 gtcgcagcaa cgttggtatc agcccaaccg agactgttcg ccatccagaa caccaggccg
    40321 atagcgccca gcatcatgat gtttgaccag ttcacggcac taagatggaa ggtgtctgcg
    40381 cgaataatat gatggcgata ctcttgcgca tcggggatgt agaggttgtt aaacacatac
    40441 tcggcgcgtt cccggttcag agtcagctct ttgcgccctt caagtacagt ttgaaaatcc
    40501 gtgtacagct tgtcttcggt ttcacgtagg gtcgccatat gtttgtacac ccgcgccacc
    40561 agcacaaaac cgccccagat ggtgatcgcc atccagatag ccgttaccag caacattttg
    40621 cccgacagca tccacagata cgccgatgaa ccgatagtga gaatgatccc ctgccccagt
    40681 tccggcagac gcacaaaagc aatggtgata ttgcgcacat cgctggttaa tcccgccagc
    40741 aacgaggcgc taccgagttg ttcaatgcgc tcgacgtggg tatccagaat ccgcttgata
    40801 aattcactac gcagtcggta aacgaagtga tgccccaaag tggtgagcgc cagttgcgat
    40861 ccgagagtga ctgccatcaa cagcagcaat aatcccagaa actccggtaa caccagcaga
    40921 ctggtatccg ccgtttcgat aaggcgctga ttgataaaag caattaagcc aatgcctaat
    40981 gccgcactgg cgaggcttag cgccatcaca ctgataaatg gccagcgata ctgccgccag
    41041 acaagtacaa gaagttccat gcagaaaacc cggacaatga attacagccc gcagtttaaa
    41101 catcttcgcg cgcacagcaa taataattct tatttttatt cttttttacc tgcctgacgg
    41161 aatgtcaggt tgtagcggca gtcggtggtg agtggatgaa accccgcttt caacggttga
    41221 ataccgtgat aaaacagccg cgattcaccg ccccatacca ccacatcgcc atgttccaac
    41281 aacaaacgtt tgagcggatc attacgtttc aggccaccaa attgaaaaat cgcgggtaag
    41341 cccagagaaa cagaaacaat tggcgcgcgc agatccggtt cgtctttatc ctgatgcagc
    41401 gacagtttcg cgccaggagc gtagcggttg ataagacaag catctggctg gaaatctgga
    41461 tagcccgccg ccgtagccgc acgttgacat aaatcatgaa agctttgtgg catagcgggc
    41521 cacggtttat ttgtttgcgg atcaatgggc gaatagagat aaccttgccg atgggtcgtc
    41581 cagcccagat gcccacagtt ggtcatcgcc accgacatgg tatatccgcc tggagcgacc
    41641 atctggcgaa acggcgactg gctggcaacg tcattaatat cgcggattag ttgctccgca
    41701 gcattaaaag caaaacgccg taaaattacc gcaccagccg ccagtggctc ttgccacggt
    41761 tcagcatcgg caaacagatc caacattacc tctcctcatt ttcagcttcg cggcgcagca
    41821 gttgcgcttt acgcgacacg ccccagcggt aaccggaaag tgtgccatca ccacggacca
    41881 cccgatgaca gggtattacg atagccagct tgttggcggc acaggcgctg gcaacggccc
    41941 gtaccgcttt cggtttgccg atggcgttag ccagttgctg ataactgacg gtttcaccac
    42001 aaggtatcgt gcgcagtgcc tgccagactt gttgctgaaa agcagtgccg cgaatatcca
    42061 gcggtaacgt cagcggcgta tcgcgttgat tgaggctggc gatcacttca cgcacatgtt
    42121 gctgaaacgt cagatcggca ggcgcgttgt cggcagcggg aaacatctgc tgcaactcgc
    42181 tgattaatgt cgcgtcatca tcgcccagca atatcgcgca aatcccccgc tcgctttctg
    42241 ccaccaggca acgacccagc tcacaatcag ccagcgcgta acgcaccgcc agattttcgc
    42301 caccgtgacg gaattgttta gccgtcatgc ccagcgtttc atcggctttg cggtagtaac
    42361 tgctgctgtc ggggaatccg gcgttaagaa tagacgtcgt cacgctctcc cctttcgcca
    42421 gcgattcgcg caaacggcga gcgcgccagg cctgttgcca ggctttaggc gtcattccgg
    42481 tagtagcttt aaacaaccga tgtagatgaa atggactcat cgccacctgg tcggctaagg
    42541 cttccagcgt tacaggcgtt tcctgttcca gcagtcgaca cgcgtgggtg attttatcca
    42601 accgatgttg ccggggattg gctttgtctg gctgacaacg tttgcagggg cgaaatccgg
    42661 cggcgagtgc ctcgctggca tttgcgtaga aggagacatt ttcccgcaag gcatgtctgg
    42721 cgcggcaaga aggacggcaa aagatacccg tggtgcgcac ggcgaaaacg aattcgccgt
    42781 cggcattcgg gtcgcgggct aagacagatt gccagcgttg atcgtcagtt aagcatgtgg
    42841 cgtttttcat aatcagctcc ctggttaagg atagccttta gtctgcccgg tcaccatcac
    42901 gcaaaaacca acaatcttgc gctttaattt ttttcgctga caaggaagct tttaaactgt
    42961 ggtgacatcc aggttttaaa gctatcgcct tctttggtga tcatataaac cgccagccct
    43021 tcccggcgaa caacctcttt ggctttctcc ggcccgagta ccatcaagcc agtatcccag
    43081 gcatcggctt ccagcgccgt cggagcaatc accgtcacgg ataccagatt gtgttcgatg
    43141 ggacgcccgg tttgcggatc gataacatgg gaaagacgtt tgccgtccag ttcgtaatag
    43201 ttacggtagc tgccagaggt gctaatccca tgaccgttga tatccaccac agcctgaacc
    43261 gcgttttctt tatcggttgg tttttgaatc gctacccgcc acggctggcc ttcaccgttc
    43321 ataccacggc tgttcagcgc gccgcccacc gacaccagat agcgggaaat cccttcctgc
    43381 tccatcaagc gtgccaggtg atccgccgca taaccttcac cgacggtgga gagatcgaca
    43441 tataaatccg gcaggtcttt ttgcagatat tgctgatgcg actgattgat gaccgtcagg
    43501 tgctgtaagc cggttttggc tttcatcgca tcgatctgtt cctggctcgg aatttgaacc
    43561 ggctgttgtt ccgggccaaa gccccacaga ttcaccagcg gcccgacggt aatatccatc
    43621 gcaccatcgg tccttgcccc aatgcgcagc gaggtggtga cgatatcggc catcgcttca
    43681 cttaccggcc acggcgacaa actttgcgag tcgttaaagc gcatcagcgc ggaatctttt
    43741 ttataggtcg aaagcagctg atcgtcggcg tccagctggg tctgaatctt ttctttaagt
    43801 tcggcactgc gtttggcatc aatgcccggg atgctggcac gccagaaggt acccatggtt
    43861 ttgccttcaa gaacggtaac ttcagtagcg tgggttttgg cgggttgtgg tttttgatcg
    43921 caaccaacaa agaagagcgc ggcaaccagc agtgccacgc gggtaaagct tatttccata
    43981 cgtgattatc ctcatgcgaa cggtcgcaag agtacaccaa aaaactgtgt ttgtacgctg
    44041 aaaacaatga aaaaagggcc cgcaggccct ttgttcgata tccatcgaga ttagaactgg
    44101 taaaccagac ccagagctac gatgttatca gtgttgatgc cagcgtcacg agtgaactgg
    44161 ttgtcgtcca gcaggttgat tttgtagtca acgtaggtgg acatgttttt gttgaagtag
    44221 taggtcgcac caacatcaac atatttcagg atatcttcgt cgtcgtagtt acgaccattg
    44281 atgacaccca ggtttttacc tttagactgc aggtaagcca gggacggacg cagaccgaag
    44341 tcgaactggt actgagcaac agcttcgaag ttctgtgctt tgttcgccca acccaagttg
    44401 cctacgcgag ttgcgttgta ggtctgggtg tactgagcag ctaggtagat gttgttcgcg
    44461 tcgtatttca gaccaccggt gtaggtttca gcgcggtcgc cattaccgat gtaacgctca
    44521 tcataacggt tcagaccgaa gttctggtca tcagtacgtt tggagctgga aactgcagca
    44581 ccgataccga agccttcgta atcataagta atagaaccgc caacgccgtc accgttctga
    44641 cgcagagctt cacgaccgtt gttggtcatg ccttcacctt ccgggctacc gtttttgccc
    44701 tgatactgaa cagcgaagtt cagaccgtca accagaccaa agaagtcggt gctacggtag
    44761 gttgcgaagc cgttaccacg ctgctgcatg aagttgtcag aaccgtaagt gtcaccaccg
    44821 aattctggca gtacgtcggt ccaggaagtt acgtcgtaaa ctacgccgta gttacgaccg
    44881 tagtcgaaag aacctacatc ctggaatttc agacctgcga atgccacacg ggtccaggag
    44941 ttgttttcgt tttcagcgct gttgccctgg atctgatatt cccactggcc gtaaccggtc
    45001 agctggtcag taacctgagt ttcacctttg aagccaagac gcatgtaggt ctggtcgcca
    45061 tctacagact tgtcgtcaga gaaatagtgc aggccgtcta ctttaccgta cagatctaat
    45121 ttgttgccgt ctttgttgta aacttcagca gcgtttgctg cgcctgctac cagcagagct
    45181 gggaccagga gggacagtac tttaactttc atgttattaa ccctctgtta tatgccttta
    45241 tttgcttttt tatgccactg catactgatt aaccctcatt aatcagtcgg caagtccatt
    45301 ctccccaaaa atgcagaata atccaacacg aatatgatac taaaactttt aagatgtttc
    45361 atttatcgct atagatgttt caaaatgtaa atacaaggga actttttaag attattgcgg
    45421 aatggcgaaa taagcaccta acatcaagca ataataattc aaggttaaaa tcaataactt
    45481 attcttaagt atttgacagc actgaatgtc aaaacaaaac cttcactcgc aactagaata
    45541 actcccgcta tcatcattaa ctttatttat taccgtcatt catttctgaa tgtctgttta
    45601 cccctatttc aaccggatgc ctcgcattcg gtttttttac ccttctttac acacttttca
    45661 ttattctgtg ctaccacaga aaaactataa cgcttgttaa ctatttcaca aataattaac
    45721 atcagcataa tttccagcaa tctttgttta tttgcaatta atttcgttgg gctttttgta
    45781 ggttatttgt acagcaaaat ggcgcttgta catctatttc cccccataca ggatgataaa
    45841 tatcacggga gaatagagaa tcatcaatca ggtaagagtc tggaatttca cactgtaccc
    45901 tttatactgc cctatcactt cgcgaagttt taacaggtca taaacacgaa tgcgtcagaa
    45961 agagacaacg gccacgaccc gcttttcact cctaccgggg agcattaccc gcttcttttt
    46021 actgttgatc attgtgttac tggtgacgat gggggtaatg gtacaaagtg ccgttaacgc
    46081 ttggctgaaa gataaaagtt accagattgt cgacattacc cacgcaatcc aaaagcgcgt
    46141 cgatacctgg cgttacgtga ccaggcagat ctacgacaac attgccgcga cgacctcccc
    46201 ctcctccggc gaaggtttac aagagacgcg cctgaaacag gatgtctact atctggagaa
    46261 accacgccgc aaaacggaag cgttaatctt tggctctcac gacaactcaa cgcttgagat
    46321 gactcagcgg atgtccactt atctggatac attgtggggt gcagaaaatg taccgtggtc
    46381 gatgtattac ctgaatggtc aggataacag tctggtgctg atctcaaccc taccccttaa
    46441 agatctcacc tccggattta aagaatcgac cgtcagtgac attgttgatt cacgtcgtgc
    46501 agaaatgtta caacaggcca acgccctcga tgaacgcgaa agtttttcta acatgcgccg
    46561 cctagcctgg cagaacggtc attactttac cttgcgtacc acgttcaacc agccgggaca
    46621 tctggcaacg gtcgtggctt ttgatctgcc gattaatgat ttgatcccac cgggtatgcc
    46681 gctggacagt ttccgccttg agccagacgc gacggcaacg ggaaacaatg ataatgagaa
    46741 agaagggacg gatagcgtca gtatccactt taacagtacg aagattgaaa tctcctcggc
    46801 actcaactct accgatatgc gcctggtctg gcaggttcct tatggcacct tattgctgga
    46861 tacgttgcaa aacattctgc tgccactgct gctgaacatc ggtttgctgg cgctggcgtt
    46921 atttggctat accacattcc gccatttctc cagccgcagt acagaaagcg tccccagcac
    46981 ggcggtcaat aacgaattgc gcattttacg ggcaatcaat gaagagatag tctcactgtt
    47041 gccgctcggc ctgctggttc acgatcagga atcgaaccgc actgtcataa gtaacaaaat
    47101 tgccgatcat ttgctgccgc atctgaatct gcaaaacatc accaccatgg cggaacagca
    47161 tcaggggatt attcaggcga cgatcaataa cgagctgtat gagatccgca tgttccgcag
    47221 ccaggtcgcg ccgcgcacac aaattttcat tattcgcgat caggatcgcg aagtgctggt
    47281 aaacaagaaa ctcaagcagg cgcagcgtct gtatgagaaa aaccagcagg ggcggatgac
    47341 ctttatgaaa aacattggcg atgcgctgaa agaacccgca cagtccctgg cggagagtgc
    47401 ggctaaactc aacgccccgg aaagcaaaca actggcgaat caggcagatg tgctggtgcg
    47461 gctggtcgaa gaaatacagt tagcgaacat gcttgcggat gatagctgga aaagtgagac
    47521 ggtgctgttc tccgtgcagg atttaattga tgaagttgtg ccttcagtgt tgcctgccat
    47581 caagcgtaaa ggtctgcaac tgctgattaa caatcatctg aaagcacacg atatgcgccg
    47641 cggcgatcgc gatgccttac gacgtatttt gctgctactg atgcaatatg ccgtgacctc
    47701 aacgcaattg ggaaaaatca cccttgaggt tgatcaggat gagtcctctg aagaccgcct
    47761 gacattccgc attctggaca cgggagaagg cgtaagtatt catgaaatgg ataatttgca
    47821 cttcccgttt atcaaccaga cccaaaacga tcgttatgga aaggcggacc cgctggcatt
    47881 ctggctgagc gatcaactgg cacgtaaact gggcggtcat ttaaacatca aaacgcggga
    47941 tgggcttggt acacgctact ctgtgcatat caaaatgctc gccgctgacc cggaagttga
    48001 agaggaagaa gagcgtttac tggatgatgt ctgcgtaatg gtggatgtta cttcggcaga
    48061 aattcggaat attgtcactc gcctgttaga aaattggggt gcaacctgta tcacacccga
    48121 tgaaagatta attagtcaag attatgatat ctttttaacg gataatccgt ctaatcttac
    48181 tgcctctggc ttgcttttaa gcgatgatga gtctggcgta cgagaaattg ggcctggtca
    48241 attgtgcgtc aacttcaata tgagcaacgc tatgcaggaa gcggtcttac aattaattga
    48301 agtgcaactg gcacaggaag aggtgacaga atcgcctctg ggcggagatg aaaatgcgca
    48361 actccatgcc agcggctatt atgcgctctt tgtagacaca gtaccggatg atgttaagag
    48421 gctgtatact gaagcagcaa ccagtgactt tgctgcgtta gcccaaacgg ctcatcgtct
    48481 taaaggcgta tttgccatgc taaatctggt acccggcaag cagttatgtg aaacgctgga
    48541 acatctgatt cgtgagaagg atgttccagg aatagaaaaa tacatcagcg acattgacag
    48601 ttatgtcaag agcttgctgt agcaaggtag cctattacat gaacaatatg aacgtaatta
    48661 ttgccgatga ccatccgata gtcttgttcg gtattcgcaa atcacttgag caaattgagt
    48721 gggtgaatgt tgtcggcgaa tttgaagact ctacagcact gatcaacaac ctgccgaaac
    48781 tggatgcgca tgtgttgatt accgatctct ccatgcctgg cgataagtac ggcgatggca
    48841 ttaccttaat caagtacatc aagcgccatt tcccaagcct gtcgatcatt gttctgacta
    48901 tgaacaacaa cccggcgatt cttagtgcgg tattggatct ggatatcgaa gggatcgtgc
    48961 tgaaacaagg tgcaccgacc gatctgccga aagctctcgc cgcgctgcag aaagggaaga
    49021 aatttacccc ggaaagcgtt tctcgcctgt tggaaaaaat cagtgctggt ggttacggtg
    49081 acaagcgtct ctcgccaaaa gagagtgaag ttctgcgcct gtttgcggaa ggcttcctag
    49141 tgaccgagat cgctaaaaag ctgaaccgca gtattaaaac catcagtagc cagaagaaat
    49201 ctgcgatgat gaagctgggc gtcgagaacg atatcgccct gctgaattat ctctcttctg
    49261 tgaccttaag tccggcagat aaagactaat catctgtagg ccagataaga cgcgttagtg
    49321 tcttatctgg catttgcacc gattgccgga tgcggcgtaa acgccttatc cggcctacga
    49381 ttcccattat ttcaacagat tacattaaag taggccagat aagacgcgtc agtgtcttat
    49441 ctggcatttg cactgaatgc cggatgcggc gtaaacgcct tatccgtcct acgaatcccg
    49501 cgatttcctg accctctcgg catataccgt cagcgtctgt tttatcacat ccagcgttac
    49561 cggcttcgag aggcagctgt ccataccgga ctccagacac cgctgcttct cttcagccaa
    49621 cgcattagca gttactccga ttaccggcaa cgtcagtccc aactgacgaa tgcgttgcgt
    49681 caagcggtaa ccatccatat ttggcatgtt gacgtcgcta agcacgatat caatatgatt
    49741 cttactaagt acattaagcg catcgacgcc atcattcgcg gttttacatt gatagcccaa
    49801 cgatcccaac tgatctgcca gcagacgtcg gttaatcgga tgatcatcca cgaccagaat
    49861 catcatatcg tcgttatcgc tgaccgcttt gtccgtcgac ggcagagcgt tagcaggatc
    49921 gtcgctctcc atctcgatca aataaatacg cgccaacaat gccggtagct catgcggagc
    49981 agccacactg tgtacccact cccctggcgc tttctccagc ggaataccaa tatggcgacg
    50041 acagaaggtc actaccgctc tgccctgcca ttttttactc actacctcgt cagtgattaa
    50101 cacatcttcg ggagtcggtt cctgcccttc gtatgttgta acgacgatgc cgctgcgctg
    50161 caaactggtt tccaggaact gacagagcga cgcattgcgg accgccagcc agcagcgttt
    50221 accactcaac ccttccacgc cttttttctg cgggtactga gcgccgtaca acggaatacg
    50281 cacggtaaac tggctgccca ttcccggttc tgaatctacc gagatatcgc cgtccatcat
    50341 gctgatcagt ttttcacaaa tcgccagacc cagaccggtc ccctggaagt ttcgctgtac
    50401 gcccgttcca acctggaaga agggatcaaa caagcgcacc acttctttcg ccggaatccc
    50461 cacgccggta tcgcgaacac ggatagagag ataatcgcca tcagcgcgaa catgcaaaac
    50521 tatacagccg gtatcggtga attttatggc gttactcaac aggttggaga tgacctgctg
    50581 taaacgcatc gggtcgccat ttaaggccac tggcacatcc ggttcaataa agcagtacaa
    50641 gcctaactgc ttgcgtacca ccagcggtaa atagttggcg gtgatgtggt tcatcacttc
    50701 acgcggtgaa aactcacgcg gttcgatctt caactgttcc gattcaatct tcgagaaatc
    50761 gagaatatcg ctgataattt tcaacaacag gctggaagag ttgttcattg ccgtcaccag
    50821 acgatcgacg cctttcggta actctttggt ttgcaacaga tccaggttac cgataatgcc
    50881 atacagcggc gttcgcagct catgactgac ggtggcaagg aacatcgatt ttgactggct
    50941 cgcctgttcc gctgcttgtg ccatctcctg caacgactct tccatcttca cgcgcgaaga
    51001 aacatccacc agcacacaaa tggccacgtt ttcattacga tagcgcgaat ggacgaagct
    51061 gatttgcaga ttggtattgt tgctggtcag gacatcaaca aaattgacct gctgcccaca
    51121 gataatttgc gtcagtcgtt ggcggtcctc atgcgtaagc atattgagat aggtatgcgc
    51181 cagttcgtta cttaaaatat tgacgccatc agcggtacgc aaaatgcaga tacccactgg
    51241 cgcggaggcg acaatcttgc gattgaactg ctcatgttct tccagtcgca gggcgtcgct
    51301 ttccgccgga atgaaaatac gtcgctcgta catccgcgcg agagtaaaca atgcagctcc
    51361 ggcaagcaca ttcagcaaaa ttgcgttaag gatcaacatg cgaatgcgtt ccagcacctt
    51421 atcaaccggc accgaataca cgatgcttag cgatgagggt ggcagatttt tcttcagcac
    51481 cagctcccgg aacccttccg tatagccaaa ccaggagcgt tcctgcatcc agcgaggatc
    51541 gcccttaatt ttactttctg gtccggtaag cgaaatcagg gtatgaccat tttcatcaag
    51601 aatggtaacc cccatcggca acgtacccgg taagaaaaag ttctccatcc ggatggtctg
    51661 ctcgacaccc aaaagcgcct gcaaccggtt cgccagataa actggcgtca acgcgtaaaa
    51721 atacccgacg caagggcgcg gaccttcgct gatccagtag aggttactgc cgctatcatc
    51781 ttgtggtgca tttcgatatt tattgatgcg ttcatgcaac gctttcaacg cggtatcgcg
    51841 ttccactggc atatcacgca gaccgaaatt ggccatgcag aggttatcac tgccgattaa
    51901 aaatacccgg ttgagatcgt aagccgcaga aaaattatcg cgccagtagc gcataaacca
    51961 cgccaatgac tccagagaac ctcgccaggt gttactcatt gcggaacaat cggagtcggc
    52021 aaacagcggt tcaaacgcag gcacatccgc ctgcgtttct cgtccacgcg gggaaagcac
    52081 accgttttct gccgataagc gattttcggc gatgtactta agctctttca tcacatcaga
    52141 ggtgcgttga ataaagcgct gagcctgatc ggaactcaga ttaaattcct gacgaatttc
    52201 cgattctcgc tgatgtaacg cattaacgat gtaaaaaacg gatgaaaaag caatcaacag
    52261 ccagagcact aacgccaatg ctctgaacat gtagcgcgag gctttcaggg ttgtacgaaa
    52321 agaagcaagg tatttcaaag gggagaagct ccgcctcagg tgaccgatgg agtgtggtta
    52381 aggtagcggt aaaagcgtgt taccgcaatg ttctgtcacc attgcaataa tgcgtatcgc
    52441 ttagaaacca ttgccttaac caggaaagcc ggagtagatg gacagttctc agtacgaaaa
    52501 ataagaaatg accaatctgc cgacgcttgt taatggttat atcctggcgt cggcagatcg
    52561 cccagcagtc ttatctgaaa aaagagagag ccaggttgtg tgaagggatg gatcaccagt
    52621 caaaactttt caccagcgtc agctcgccag cattacgcat cggtacaata aatgtttcct
    52681 gtttctcatt gaccgatcct tcatcggtga tcagcgtcag ttgggcggtc gtcaattccg
    52741 tttcgctgcg cccaccatag tagttgatat acacctgata gcgcccgtga actggtgctg
    52801 gcatggcgaa aatctcgggt ccgtaccccg tcgtgacatc catatccagt gcaccactgt
    52861 ttttcagcac ggtgttaccg taccaggcgt gttcgccatc gggcgtgaca acatggagat
    52921 cgagatcggt attgtccgta tcccacgaga gaaccagccg taaacgtgca cgaatagttc
    52981 ctgtacccgg cgttgagtaa aactgcattt tttgccggct ttgcccatcc gggctgatga
    53041 cctgcacgct gttgctgcct tcagtgaaaa tataagggcg tgcaaatgaa ccatcggatt
    53101 caattctctg cggcatactg gcaccgttga ccaccagccg gccctgctga actttacccg
    53161 ccgcagcata attctttatt ttgccgcgga tctgcgctga gatattttga tcatctgcca
    53221 tgttgacgga cgacgccggg taattgatcg attgtgaaaa actggcatct tcgccctctg
    53281 ccggatgcca gccagagagc ggtgcgtcaa tctcgacttc ctgcacacct tcactgtgag
    53341 cgacaggcga aagtgccacc agtaacaacg gaagaaaaat ctttcgcata gcattagtcc
    53401 attaataatt gtcgggtcag cgtttcgata tagttctcat caagcccggt gggataacca
    53461 tcaaaggcca agtgcagata ctcatgggtt aaatccagcc gatcctgaag cgtaaagaag
    53521 ttacgaataa acagccgttt ctgctgccga tcggtatagg ggaaacctga gactaaacga
    53581 cagaccgcaa acacgtctgg ttcgttgtac cccgtctcgc cttgtaatat acgccgccac
    53641 tgcggcattt ttttcgccag ccaggctttc gctttgggta ataactggca ggttgagcgt
    53701 ggagctcccc agcggctaag gctgttgtcg ggataagcaa acgcgagtat ctggtcgtaa
    53761 cgctccccct gccccgcctg ggccgtggct tgccgccagg aaagtgtccc ttcggtagcg
    53821 cgactgccat gataatgaac cggatcgcct gcatagatga gctcctgcgt ccaggcggtc
    53881 atcgttcgcg cccccgtggt cgctggcgaa gcagaaacac gctgcgtggc gctgctgtcg
    53941 gggatggtca ggcaatcacc ttcccgattg gcgttttgct gtaaaaaagt acgtatcgcc
    54001 acagtcatgg ctttcgctgc ctcaggtggc gtacttttcg cctcgcgatc cagaactctg
    54061 gcaacgtact cttcacgatc gagatgcgac tgcaatttca gtttgccctt ttcgctcagt
    54121 aaggtcgtct caccgtggct gacaaacgta atgtggttac cattagtaaa cgtcacacga
    54181 tagcgcccat taagtacgct gggttttact gccgtcgtac tcttttccgc tgtgattttc
    54241 ttcagcggat agcgggcaaa cagctccacc tctacacatt gcccactggc aacctgcgtc
    54301 ggcactggca aaacccgatt aagcaccgtc gcataacggg ttagtaccgt tttgctggtg
    54361 ccactgccag taacccacag cggtgtacca tcggttaacc agccagcaaa accaccctga
    54421 cggatttcct gtttatcatc ggcaaaccag ctccaggttt tcacccgtac ccgactaccg
    54481 agcatggatg cgacaccgat tttcgcctcg tccagcacca catccagtaa gacttcctga
    54541 gctttgtttt gcgcaggtaa cgtcgctaat gaatcaagca gcgatttaac cgttaccgag
    54601 gtttctggct tcagcgttgt aagagacgct aaccacgcag gtgcctgacg ctgttgccag
    54661 tattgccccc acacatctgc gccaatatgc aaccgctgcg gcgcaaaata gagcccacag
    54721 gaacgtacca gcgcggtatc gcgcgttatg ctctccccag cctgacaaca ataaacctct
    54781 tccggggagt ttccgcgaca ctggtagggc tgctccggtt gatgggtatc ctccagccag
    54841 gcgtaaacat acagtttcca caaactccct aacggcgtgg tgagcgagtc aggtaatgct
    54901 gagactttgg tcacgcctga tgacgacagc tgatacagtt gatcgtgctg cgcaccgcgc
    54961 agcaccagtt gtaacggcgt ctcctccgca agcgttggca gagtaaccaa cgccaatagc
    55021 cagacgattc tgcgccagtt cacttatttc acctgcatcc cggtccattc actccctgcc
    55081 gcgacacttt gctgcgcagg tgcataggaa cgcacataac gagcaggagg cagaacgaat
    55141 tgcccttttt gcgagaagcg cagcaaatgg cggaaagtga ccgttcccgt cagttctttc
    55201 accggcacca tataggccag ttcgcccatt tcattacgcg ctttttccag caattgcccc
    55261 tgctgtttcg cggcgttggg tttattgact gaaatgcccc atgttgtgcg ctcaacgtca
    55321 gctcccggcg ggagcggtac ttccacctga ccatagcgca gaactgcatc ctgctcgctg
    55381 gtaagcgtaa tttcatcgag atacagcgca tcgctgtcaa tctcattgct ggtcaccggc
    55441 tgcagagtaa agctcatctc ttcttcaccg gggataagcc gatacaactg gcgttcaacg
    55501 gtcaccggaa tgttactttg ttgagccgtt tttgccggtt cacgccagcg gacctgcaca
    55561 tttgcggcga taattcgtca ccaaaagaga gaatgtccgg cacaccctaa ccaacccaac
    55621 gccagtcttc gccccctcca gttaacttat gtttagccca tgcgcccgca ggcgcaggca
    55681 aaacaactgg aggcattgtc gccatatatt tcgccagcca gttcatggcc agcgcgcgct
    55741 caatagtgga ttgctcagcg gtcaaaccat taaaatagcg gccgtctggg tagcgtcacc
    55801 accgctattg agcagcacaa ccgtgtgcgc cagcggctga ttgctgtttg ccgctttatc
    55861 ccacgcatat tgctgaacct gtggcatcac cgtggacttc aactgcgctt ttttcagcaa
    55921 cgtcatggtt aagacgtttg ccaccgcatc tgccagtgga gattccggtg tatcgaggat
    55981 caggctgtca ttgatatcgc ggatgtcttc ctcagagaaa tcttcatctt tagttccgcg
    56041 ccgggcgata gcttcatccc cccctttcaa caacgttttg cacggcagat tcatctcctg
    56101 tgcccacgcc agcaccagcg cccgatgcaa taacggcata ttatccgcct gctcggcata
    56161 actgtcgagc atatgctgcc agtattccgg ttgttgcgtt acgccgagcg cctggctggc
    56221 ctgccagtcg gcgtaccatg cccatgccgt aaggaaggcg tcaccattgc catcttcacc
    56281 ccaccaggta aagcgcgctc cgggccccgc cagttgcatc agccgcagac ggttatcctg
    56341 aatcatctga cgaatgtcgt tagcggcggc actttgatga tcggcaagcg aacgccaggc
    56401 gagactgagc gggatcagac ggctaccggt gttgatcacc ccaccccacg gttcatcgac
    56461 taacgcatca agattgttgc ggaaaatctc ctgcggcgtt tcactacttt gtagccggat
    56521 attactcgcc tgctcgggca acatcagcgc gttctcgcca ccgccgagca tgacattttt
    56581 ctgctgttca acgggccagc tgttgtccac aaaagacagt tttgtgctaa tgctgtcctg
    56641 cacttgccca ttttgttgca gttctgcact taacaagcca gattgctgaa tattctgcgt
    56701 cagcgaaata taattcgccc ctttgtgcag cgtcagcgtc tggcgcatct cagcgcctgc
    56761 aaatttagtc accagcgcca ccggttcgtt atcctgctga ctgaagataa acagtcctgc
    56821 cgccggtttg tcgcccacgc gatacaccgt tggcatactc cacttcatgt agagattttt
    56881 ttccgaacgc agataagcac gcccctgccc gaccagcccg tcgccgttca tcccacgcgc
    56941 ggtgatacgc cagcgggtta acgaatcagg catcaggaac gtgaaatacg ctttgccttg
    57001 tttatcggtt gtgagtgacg gcatccatgc cgcggtatcc acctcttcac gccgtggacg
    57061 ttcaagcatt tttactcgcc gctcgctgcg gttagtcgcg ccaggcgcaa ccggctcgct
    57121 ggagagcgcc tggtcatagc tgataaacga cagactggag ctggtacgca cattgttacg
    57181 ccccagcgga tagaaaaatt tgccgatatt cggcgcgatt tctggttgca gcgcgtagat
    57241 catttcatcg accacgccta ccgttagctg cgcagaaaca ggtttacctt tcagcgacga
    57301 ggttaatt
//
LOCUS       DAJTUP010000014        54405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTUP010000014 DAJTUP010000000
VERSION     DAJTUP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.014"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1022)
                     /gene="argH"
                     /locus_tag="OO468_000876"
     CDS             complement(<1..1022)
                     /gene="argH"
                     /locus_tag="OO468_000876"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR6258093.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLH"
     gene            complement(1083..1859)
                     /gene="argB"
                     /locus_tag="OO468_000877"
     CDS             complement(1083..1859)
                     /gene="argB"
                     /locus_tag="OO468_000877"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR6258094.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(1867..2871)
                     /gene="argC"
                     /locus_tag="OO468_000878"
     CDS             complement(1867..2871)
                     /gene="argC"
                     /locus_tag="OO468_000878"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR6258095.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3025..4176
                     /gene="argE"
                     /locus_tag="OO468_000879"
     CDS             3025..4176
                     /gene="argE"
                     /locus_tag="OO468_000879"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR6258096.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4528..7179
                     /gene="ppc"
                     /locus_tag="OO468_000880"
     CDS             4528..7179
                     /gene="ppc"
                     /locus_tag="OO468_000880"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR6258097.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7362..9095
                     /locus_tag="OO468_000881"
     CDS             7362..9095
                     /locus_tag="OO468_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR6258098.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9244..10095
                     /locus_tag="OO468_000882"
     CDS             9244..10095
                     /locus_tag="OO468_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6258099.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10082..10423)
                     /locus_tag="OO468_000883"
     CDS             complement(10082..10423)
                     /locus_tag="OO468_000883"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6258100.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10425..11303)
                     /locus_tag="OO468_000884"
     CDS             complement(10425..11303)
                     /locus_tag="OO468_000884"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR6258101.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11269..13566)
                     /locus_tag="OO468_000885"
     CDS             complement(11269..13566)
                     /locus_tag="OO468_000885"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR6258102.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(13617..13937)
                     /locus_tag="OO468_000886"
     CDS             complement(13617..13937)
                     /locus_tag="OO468_000886"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR6258103.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(13952..15031)
                     /locus_tag="OO468_000887"
     CDS             complement(13952..15031)
                     /locus_tag="OO468_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR6258104.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15340..16443
                     /locus_tag="OO468_000888"
     CDS             15340..16443
                     /locus_tag="OO468_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR6258105.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16444..17841
                     /gene="ptsP"
                     /locus_tag="OO468_000889"
     CDS             16444..17841
                     /gene="ptsP"
                     /locus_tag="OO468_000889"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6258106.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            17853..18515
                     /gene="fsa"
                     /locus_tag="OO468_000890"
                     /pseudo
     CDS             17853..18515
                     /gene="fsa"
                     /locus_tag="OO468_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18526..19627
                     /gene="gldA"
                     /locus_tag="OO468_000891"
                     /pseudo
     CDS             18526..19627
                     /gene="gldA"
                     /locus_tag="OO468_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            19902..20509
                     /locus_tag="OO468_000892"
                     /pseudo
     CDS             19902..20509
                     /locus_tag="OO468_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(20536..21441)
                     /gene="yijE"
                     /locus_tag="OO468_000893"
     CDS             complement(20536..21441)
                     /gene="yijE"
                     /locus_tag="OO468_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR6258107.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21535..23715)
                     /gene="katG"
                     /locus_tag="OO468_000894"
     CDS             complement(21535..23715)
                     /gene="katG"
                     /locus_tag="OO468_000894"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR6258108.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24044..24934)
                     /gene="metF"
                     /locus_tag="OO468_000895"
     CDS             complement(24044..24934)
                     /gene="metF"
                     /locus_tag="OO468_000895"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR6258109.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25283..27715)
                     /gene="metL"
                     /locus_tag="OO468_000896"
     CDS             complement(25283..27715)
                     /gene="metL"
                     /locus_tag="OO468_000896"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR6258110.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(27718..28878)
                     /gene="metB"
                     /locus_tag="OO468_000897"
     CDS             complement(27718..28878)
                     /gene="metB"
                     /locus_tag="OO468_000897"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR6258111.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29155..29472
                     /gene="metJ"
                     /locus_tag="OO468_000898"
     CDS             29155..29472
                     /gene="metJ"
                     /locus_tag="OO468_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR6258112.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            29656..30264
                     /locus_tag="OO468_000899"
     CDS             29656..30264
                     /locus_tag="OO468_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR6258113.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30325..30537)
                     /gene="rpmE"
                     /locus_tag="OO468_000900"
     CDS             complement(30325..30537)
                     /gene="rpmE"
                     /locus_tag="OO468_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR6258114.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            30740..32938
                     /gene="priA"
                     /locus_tag="OO468_000901"
     CDS             30740..32938
                     /gene="priA"
                     /locus_tag="OO468_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR6258115.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33094..34119
                     /gene="cytR"
                     /locus_tag="OO468_000902"
     CDS             33094..34119
                     /gene="cytR"
                     /locus_tag="OO468_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR6258116.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34211..35170
                     /gene="ftsN"
                     /locus_tag="OO468_000903"
     CDS             34211..35170
                     /gene="ftsN"
                     /locus_tag="OO468_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR6258117.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35263..35793
                     /gene="hslV"
                     /locus_tag="OO468_000904"
     CDS             35263..35793
                     /gene="hslV"
                     /locus_tag="OO468_000904"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR6258118.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            35803..37134
                     /gene="hslU"
                     /locus_tag="OO468_000905"
     CDS             35803..37134
                     /gene="hslU"
                     /locus_tag="OO468_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR6258119.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37201..38127
                     /gene="menA"
                     /locus_tag="OO468_000906"
     CDS             37201..38127
                     /gene="menA"
                     /locus_tag="OO468_000906"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR6258120.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38220..38705
                     /gene="rraA"
                     /locus_tag="OO468_000907"
     CDS             38220..38705
                     /gene="rraA"
                     /locus_tag="OO468_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR6258121.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(38790..39035)
                     /gene="zapB"
                     /locus_tag="OO468_000908"
     CDS             complement(38790..39035)
                     /gene="zapB"
                     /locus_tag="OO468_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR6258122.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39461..40306
                     /gene="glpF"
                     /locus_tag="OO468_000909"
                     /pseudo
     CDS             39461..40306
                     /gene="glpF"
                     /locus_tag="OO468_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40346..41836
                     /gene="glpK"
                     /locus_tag="OO468_000910"
     CDS             40346..41836
                     /gene="glpK"
                     /locus_tag="OO468_000910"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR6258123.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42066..43076
                     /gene="glpX"
                     /locus_tag="OO468_000911"
     CDS             42066..43076
                     /gene="glpX"
                     /locus_tag="OO468_000911"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6258124.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43173..43919
                     /gene="fpr"
                     /locus_tag="OO468_000912"
     CDS             43173..43919
                     /gene="fpr"
                     /locus_tag="OO468_000912"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR6258125.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(43924..44352)
                     /gene="uspD"
                     /locus_tag="OO468_000913"
     CDS             complement(43924..44352)
                     /gene="uspD"
                     /locus_tag="OO468_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR6258126.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44379..44678)
                     /locus_tag="OO468_000914"
     CDS             complement(44379..44678)
                     /locus_tag="OO468_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR6258127.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(44890..45330)
                     /locus_tag="OO468_000915"
                     /pseudo
     CDS             complement(44890..45330)
                     /locus_tag="OO468_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45431..46030
                     /locus_tag="OO468_000916"
     CDS             45431..46030
                     /locus_tag="OO468_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR6258128.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46138..46905
                     /gene="tpiA"
                     /locus_tag="OO468_000917"
     CDS             46138..46905
                     /gene="tpiA"
                     /locus_tag="OO468_000917"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR6258129.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(46960..47715)
                     /gene="cdh"
                     /locus_tag="OO468_000918"
     CDS             complement(46960..47715)
                     /gene="cdh"
                     /locus_tag="OO468_000918"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR6258130.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(47822..48811)
                     /gene="sbp"
                     /locus_tag="OO468_000919"
     CDS             complement(47822..48811)
                     /gene="sbp"
                     /locus_tag="OO468_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR6258131.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49131..50093)
                     /gene="pfkA"
                     /locus_tag="OO468_000920"
     CDS             complement(49131..50093)
                     /gene="pfkA"
                     /locus_tag="OO468_000920"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR6258132.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50274..51176)
                     /gene="fieF"
                     /locus_tag="OO468_000921"
     CDS             complement(50274..51176)
                     /gene="fieF"
                     /locus_tag="OO468_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR6258133.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51324..51824)
                     /gene="cpxP"
                     /locus_tag="OO468_000922"
     CDS             complement(51324..51824)
                     /gene="cpxP"
                     /locus_tag="OO468_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR6258134.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            51974..52672
                     /gene="cpxR"
                     /locus_tag="OO468_000923"
     CDS             51974..52672
                     /gene="cpxR"
                     /locus_tag="OO468_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR6258135.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            52669..54042
                     /gene="cpxA"
                     /locus_tag="OO468_000924"
     CDS             52669..54042
                     /gene="cpxA"
                     /locus_tag="OO468_000924"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR6258136.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54148..>54405)
                     /locus_tag="OO468_000925"
     CDS             complement(54148..>54405)
                     /locus_tag="OO468_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR6258137.1"
                     /translation="GKVGWLYSVIAPGKVSADAPLELVSRVSDVTVQEAAAIAWHMPF
                     DDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFSRRLWGK"
BASE COUNT        13263 a        14714 c        13932 g        12496 t
ORIGIN      
        1 atatgcaggc agtccagcca ggtatcgagc gcgtcgaaca gaccttcttt gtcttcctgc
       61 atatctttgt tgtaagccag cggcaaacct ttcagcgtca tcatcatgcc ggttaacgca
      121 ccctgcaccc ggccgcattt accgcgaatc agctccagcg catccgggtt tttcttctgc
      181 ggcattaatg atgaaccgga agtcacgcgg tccgaaagct ccacaaaccc cgcttcgccg
      241 gtgttaaaga aaatcagatc ttcagcaaaa cgcgacagat gcaccatgcc gatagcggca
      301 gcagaaagca gttccaacac atggtcacgg tcagaaacgc tgtccagact gttacgggtc
      361 gccgaagcaa agcccagcca gcctgctaac tgttcacggt cgatttcata ggccgttccc
      421 gccagcgcgc cacagcctaa tgggctgaca tccagacgct taagcgcatc ctgcaaacgg
      481 ctttcatcac gcgccagcat ctcaacatag gccaggcacc agtgcgcgaa cgtcaccggc
      541 tgggcgcgtt gcaggtgagt gtaacctggc attaccgcgt cctgattgtt ttgtgcggtt
      601 tccaccagcg ccgattgcag ctgccggtta gccgtcagta actcgctaac ggtatctttg
      661 caccacagtt tcaggtcagt cgctacctga tcattacggc tacgcccggt atgcagcttt
      721 ttgcctaact ggcccacttt gtcgatcagt ttgccttcca cccagctatg gatatcttcg
      781 gcgtcgcttt caaggatttg ttgtggcctg gcgcgaacat cttccagcaa cacgttcagc
      841 gcctcttcca gttgcgcctg ctcttctgcg gttaacacgc cgaccgttac caaggctttg
      901 gaccaggcca cagagccaac aatatcctgc tccgccagac ggtaatcaaa gcgcagtgag
      961 tcgttgaatt gtttgaaccg ttgatctgct gcctgggtaa aacgcccgcc ccaaagtgcc
     1021 ataactctgt ttccttattt tgaaattcaa tgccggaaag ctgcgcctcc ggctaacaaa
     1081 acttaagcta aaatccgcgt acccatcggc ataccgttaa acagtgccgg aagctgctcc
     1141 gcataacgcc aagaagcgat atctaccgga cggcccagcg tgcgggccgc atccagcgcc
     1201 gcgttcactt tcactatcat gccgtcagta ataatgccct gctcaatcag ttgttctgct
     1261 ttcgcggcgg tcatttcggc aatgcgttgc cctttgccgt cgagaatgcc gctgacgtcg
     1321 gagagcaaaa tcagatccgc gcccagcgtt gccgccagcg ccgttgccgc ctggtcggca
     1381 ttgacgttca tcagttgccc ttcgtctgtt acgccaatgg agctgaccac cggcagataa
     1441 ccgttctcca gcaaggagtt gataagctta ggcgaacctg gctgcgccag tccaacatga
     1501 cctaactctt catcaagctg ggtcactttg acgctgtcgc cgtcaccgag aaacaaacct
     1561 acggccgcaa tctgatgttt cttcgcccat gccaacaggg ttttatttgc cgttcccgcc
     1621 agtgctccgg tgataatgtc tatctgatca gcaggcgtca cccgcaggcc gtttttcttt
     1681 ttcaccggca gattcagccc tttcatcagc tcatccacca cgcaaccgcc gccgtgcaca
     1741 atcaccagcg gacgctgatg tgactcacga taattcacca atgcgctaaa cagacgttcc
     1801 agcgcctctt cactatccag cagtacgccg cccagtttga taattaatgg attcatcatt
     1861 gcacccttaa ataagagact gcgtttccgc atagccgaaa cgaatattgg cgcactgtac
     1921 cgcttgtgcc gccgcgcctt tcagtaagtt gtcttcggtc gccacaataa tcagatgctc
     1981 gccctgaacg gcaaacccga tatcgcaaaa tggcagccca acgacatttt tcagcgccgg
     2041 aacgcctttg tcatacagcc gcaccagcgg tttatgggca tacgcctgct gtaacgcttg
     2101 cgcgacttgc gcctgggtca cacccgattt caggcggcag gtaatggttt cgagaatgcc
     2161 gcgcgggaaa ttgcccagat gtggggtgaa gataacgtca gcaccgaggt gtgtggcgat
     2221 ctctggttga tggcgatgag taaagacgcc atacggttgc aggctaactt cacaaaaact
     2281 atttgaaatc gctgccttac gtcctgcacc gctcacgccg ctggtggcgt tgatcaccgg
     2341 ccactgattg aggtcaagaa gatcggcatc aatcaacggt ttcagcgcca gctgtgccgc
     2401 cgtcggataa cagcctggca ccgcaatcaa attcgcttct tttaatttat taccgcacca
     2461 ctccgccaga ccgtaggctg cctgttccag cagttccggg tattgatggg taaagccgta
     2521 atatttttca tagaaggtgg cgtcgttaac acgaaacgcg ccggaaaggt cgaacaccac
     2581 gcagcccgct tcaagaaact gcggcgctaa atcgtggcta acttcatggg cggtggcgag
     2641 aaacactacg tccacccctg ggctaaactc gctgatatcc gacatcggct gcaacggcag
     2701 ctcaacgatg ccttttagct gcggatgcaa atcggagatt aactttcccg catcattgct
     2761 ttgcgctgaa acagtcaaag cggttatgtt catatgcgga tggcgattta cataggtcac
     2821 tagctctgcg ccagcgtagc cgctggcacc cacaatcagc gtattcaaca tcggggctat
     2881 tcaccttctt atgtctggtt gccaggttaa acgtaaaaca ttcaccttac ggctggtggg
     2941 ttttattacg ctcaacgtta gtgtattttt attcataaat actgcatgaa tattgatact
     3001 atcatgacca gaggtgtgtc aacaatgaaa aacaaattac cgccatttat cgagatttac
     3061 cgcgctctga ttgccacacc ttcaataagc gctacggaag aggcactcga tcaaagcaat
     3121 gcagatttaa tcactctgct ggcggactgg tttaaagatt tgggcttcaa tgtggaagtg
     3181 cagcctgttc caggaactcg caacaaattt aatatgctgg ccagttacgg acagggggct
     3241 ggcggcttgt tgctggcggg gcataccgat acggtgccat ttgatgacgg tcgctggacg
     3301 cgcgatccgt ttacactgac ggagcatgac ggcaagcttt acggcttagg caccgccgac
     3361 atgaaaggct tttttgcgtt tatccttgat gcgctacgcg atgtcgacgt cacgaaactg
     3421 aaaaaaccgc tctacattct ggcgactgct gatgaagaaa ccagtatggc cggagcgcgt
     3481 tattttgccg aaactaccgc cctgcgcccg gattgcgcca tcattggcga accgacgtca
     3541 ctacaaccgg tacgcgcaca taaaggtcat atctctaacg ccatccgtat tcagggccag
     3601 tcggggcact ccagcgatcc agcacgcgga attaacgcta tcgaactaat gcacgacgcc
     3661 atcgggcata ttttgcaatt gcgcgataac ctgaaagaac gttatcacta cgaagcattt
     3721 accgtgccat accctacgct caacctcggg catattcacg gtggcgacgc ttctaaccgt
     3781 atttgcgctt gctgtgagtt gcatatggat attcgtccgc agcctggcat gacactcaat
     3841 gaacttaatg gtttgctcaa cgatgcattg gctccggtga gcgaacgctg gccgggtcgt
     3901 ctgacggtcg acgagctgca tccgccgatc cctggctatg aatgcccacc gaatcatcaa
     3961 ctggttgaag tggttgagaa attgctcgga gcaaaaaccg aagtggtgaa ttactgtacc
     4021 gaagcgccgt ttattcaaac gttatgcccg acgctggtgc tcggtccggg ttccatcaat
     4081 caagctcatc agcctgatga atatctggag acgcggttta ttaaacccac ccgtgagttg
     4141 attatccagg taattcacca tttttgctgg cattaaaacg taggccggat aaggcgctcg
     4201 cgccgcatcc ggcaccgttg ccaaactccg gtgccgcaat aatgtcggat gcgatgcttg
     4261 cgcatcttat ccgacctaca cctttggtgt tatgtatggc gattttttaa catttccata
     4321 agttacgctt atttaaagcg tcgtgaattt aatggcgtaa attcctgcta tttattcgtt
     4381 tgctgaagcg atttcgcagc atttgacgtc accgctttta cgtggcttta taaaagacga
     4441 cgaaaagcaa agcccgagca tattcgcgcc aatgcgacgt gaaggataca gggctatcaa
     4501 acgataagat ggggtgtctg gggtaatatg aacgaacaat attccgcatt gcgtagtaat
     4561 gtcagtatgc tcggcaaagt gctgggagaa accatcaagg atgcgttggg agaacacatt
     4621 cttgaacgcg tagaaactat ccgtaagttg tcgaaatctt cacgcgctgg caatgatgct
     4681 aaccgccagg agttgctcac caccttacaa aatttgtcga acgacgagct gctgcccgtt
     4741 gcgcgtgcgt ttagtcagtt cctgaacctg gccaacaccg ccgagcaata ccacagcatt
     4801 tcgccgaaag gcgaagctgc cagcaacccg gaagtgatcg cccgcaccct gcgtaaactg
     4861 aaaaaccagc cggaactgag cgaagacacc atcaaaaaag cagtggaatc gctgtcgctg
     4921 gaactggtcc tcacggctca cccaaccgaa attacccgtc gtacactgat ccacaaaatg
     4981 gtggaagtga acgcctgttt aaaacagctc gataacaaag atatcgctga ctacgaacgc
     5041 aaccagctga tgcgtcgcct gcgccagttg atcgcccagt catggcatac cgatgaaatc
     5101 cgtaagctgc gtccaagccc ggtagatgaa gccaaatggg gctttgccgt agtagaaaac
     5161 agcctgtggc aaggcgtacc gaattacctg cgcgaactga acgaacaact ggaagagaat
     5221 ctcggctaca aactgcccgt cgaatttgtt ccggtacgtt ttacctcgtg gatgggcggt
     5281 gaccgcgacg gcaacccgaa cgtcactgcc gatatcaccc gccacgtcct gctgctcagc
     5341 cgctggaaag ccaccgatct gttcctgaaa gatattcagg ttctggtttc tgaactgtcg
     5401 atggttgaag cgacccctga actgctggcg ctggttggcg aagaaggtgc cgcagaaccg
     5461 tatcgctatc tgatgaaaaa cctgcgttct cgcctgatgg cgacacaggc atggctggaa
     5521 gcgcgcctga aaggcgaaga actgccaaaa ccagaagggc tgctgacaca aaacgaagaa
     5581 ctgtgggaac cgctctacgc ttgctaccag tcacttcagg cgtgtggcat gggtattatc
     5641 gccaacggcg atctgctcga caccctgcgc cgcgtgaaat gtttcggcgt accgctggtc
     5701 cgtattgata tccgtcagga gagcacccgc cataccgaag cgctgggcga gctgacccgc
     5761 tacctcggta tcggcgacta cgaaagctgg tcagaggccg acaaacaggc gttcctgatc
     5821 cgcgaactga actccaaacg tccgcttctg ccgcgcaact ggcaaccaag cgccgaaacg
     5881 cgcgaagtgc tcgatacctg ccaggtgatt gccgaagcgc cgcaaggctc cattgccgcc
     5941 tacgtgatct cgatggcgaa aacgccgtcc gacgtactgg ctgtccacct gctgctgaaa
     6001 gaagcgggta tcgggtttgc gatgccggtt gctccgctgt ttgaaaccct cgatgacctg
     6061 aacaacgcca acgatgtcat gacccagctg ctcaatatcg actggtatcg cggcctgatt
     6121 cagggcaaac agatggtgat gattggctat tccgactcag caaaagatgc gggcgtgatg
     6181 gcagcttcct gggcgcaata tcaggcacag gatgcattaa tcaaaacctg cgaaaaagcg
     6241 ggtattgagc tgacgttgtt ccacggtcgc ggcggttcca ttggtcgcgg cggcgcacct
     6301 gcccatgcgg cgctgctgtc acaaccgcca ggaagcctga aaggtggcct gcgcgtgact
     6361 gaacagggcg agatgatccg ctttaaatat ggtctgccag aaatcaccgt cagcagcctg
     6421 tcgctttaca ccggggcgat tctggaagcc aacctgctgc caccacctga gccgaaagag
     6481 agctggcgtc gcattatgga tgaactgtca gtcatctcct gcgatctcta ccgtggctac
     6541 gtacgtgaaa acaaagattt tgtgccttac ttccgctccg ctacgccgga acaagaattg
     6601 ggcaaactgc cgttgggttc acgtccggcg aaacgtcgcc caaccggcgg cgtcgagtcg
     6661 ctgcgcgcca taccgtggat ttttgcctgg acgcaaaacc gcctgatgct ccccgcctgg
     6721 ctgggtgcag gtacggcgct gcaaaaagtg gtcgaagatg gcaaacagag cgagctggaa
     6781 gccatgtgcc gcgattggcc attcttctcg acgcgtctcg gcatgctgga gatggtcttc
     6841 gccaaagcag acctgtggtt ggcggaatac tatgatcaac gtctggtaga caaagcactg
     6901 tggccgttag gtaaagagtt acgcaacctg caagaagaag acatcaaagt ggtgctggcg
     6961 attgccaacg attcccatct gatggccgat ctgccgtgga ttgcagagtc tattcagcta
     7021 cggaatattt acaccgaccc gctgaacgta ttgcaggccg agttgctgca ccgctcccgc
     7081 caggcagaaa aagaaggcca ggaaccggat cctcgcgtcg aacaagcgtt aatggtcact
     7141 attgccggga ttgcggcagg tatgcgtaat accggctaat cttcctcttc tgcaaaccct
     7201 cgtgcttttg cgcgagggtt ttctgaaata cttctgttct aacaccctcg ttttcaatat
     7261 atttctgtct gcattttatt caaattctga atataccttc agatatcctt aaggaattgt
     7321 cgttatattc ggcgatattt ttttcaagac aggttcttac tatgcattcc acagaagtcc
     7381 aggctaaacc cctttctagc tggaaagccc tgggttgggc actgctctac ttttggtttt
     7441 tctctactct gctacaggcc attatttaca tcagtggtta tagtggcacc aacggcattc
     7501 gcgactcgct gttattcagc tcgctgtggt tgatcccggt attcctcttt ccgaagcgga
     7561 ttaaaattat tgccgcagtg atcggcgtgg tgctatggac ggcctctctg gcggcgctgt
     7621 gctactacgt catctacggg caggagttct cgcagagcgt tctgtttgtg atgttcgaaa
     7681 ccaacaccaa cgaagccagc gagtatttaa gccagtattt cagcctgaaa attgtgctta
     7741 tcgcgctggc ctatacggcg gtggcagttc tgctgtggac acgcctgcgc ccggtctata
     7801 ttccaaagcc gtggcgttat gttgtctctt ttgccctgct ttatggcttg attctgcatc
     7861 cgatcgccat gaatacgttt atcaaaaaca agccgtttga gaaaacgttg gataacctag
     7921 cctcgcgtat ggagcctgcc gcaccgtggc aattcctgac cggctattat cagtatcgtc
     7981 agcaactaaa ctcgctaaca aagttactga atgaaaataa tgccttgccg ccactggcta
     8041 atttcaaaga tgaatcgggt aacgaaccgc gcactttagt gctggtgatt ggcgagtcga
     8101 cccagcgcgg acgcatgagt ctgtacggtt atccgcgtga aaccacgccg gagctggatg
     8161 cgctgcataa aaccgatccg aatctgaccg tgtttaataa cgtagttacg tctcgtccgt
     8221 acaccattga aatcctgcaa caggcgctga cctttgccaa tgaaaagaac ccggatctgt
     8281 atctgacgca gccgtcgctg atgaacatga tgaaacaggc gggttataaa accttctgga
     8341 tcaccaacca gcagacgatg accgcccgca ataccatgct gacggtattt tcgcgccaga
     8401 ccgacaagca gtactacatg aaccagcaac gtacgcagag tgcgcgtgaa tacgacacca
     8461 acgtgctgaa gccgttccag gatgtgctga atgaccctgc gccgaagaaa ctgatcatcg
     8521 ttcatctgct gggtacgcat atcaaataca aataccgcta cccggaaaat cagggcaagt
     8581 ttgatggcaa taccgatcat gttccgccag gattaagcgc agaagagctg gaatcatata
     8641 acgattatga caacgctaac ctgtataacg atcatgtggt tgccagcctg attaaagact
     8701 ttaaagcggc agacccgaac ggattcctgg tttacttctc tgaccacggt gaagaggttt
     8761 acgacacgcc gccgcacaaa actcaggggc gtaacgaaga caacccgacg cgccacatgt
     8821 acaccattcc gttcctgctg tggacgtcgg aaaaatggca agcgactcat ccccgtgatt
     8881 tctcacagga tgtcgatcgt aaatacagcc tggcggaact gatccacacc tggtcagatt
     8941 tggcgggctt atcttacgac ggttacgatc caacccgttc agtggtgaat ccgcagttca
     9001 aagaaactac ccgctggatt ggtaacccgt acaagaaaaa cgcgctgatc gattacgaca
     9061 ctctgccgta tggcgatcag gtgggtaatc agtagatgac ctttgtatcg cgtctggcgc
     9121 tatttgccgg atgcgatact gccgcaatgt ttacatcgaa ctcccctcag gccacacttt
     9181 tcaggtggcc ttttttaata atgccagtta ctgttttccc gtctacatac ctaagggaat
     9241 aacatgtatc acgacgtcag ctatctgctc tcccgcctga tcaacggccc gctgtccctg
     9301 cgccagattt actttgccag cagtaacggc ccggtgcccg atcttgcgta tcaggtcgat
     9361 tttccacggc tggaaattgt gctggaaggt gagtttgttg ataccggcgc tggagcaacg
     9421 ttagttcccg gcgatgtgct gtacgttgcc gctggtggct ggaatttccc acaatggaaa
     9481 acacccgcta ccacctttag cgtgctgttt ggcaaacagc aactcggctt cagcgtcgtg
     9541 caatgggatg gcaaacaata tcaaaatctg gcgaagcaac acgtcgcccg acgcggccca
     9601 cgcataggtt cttttctgct acaaacgctc aatgaaatgc aaatgcagcc gcaggagcag
     9661 caaacggcaa ggcttatcgt agccagcctg cttagccact gccgcgattt gctcggcagc
     9721 caaatccaga ccgcctcacg cagccaggca ctatttgaag ctattcgcga ttatatcgac
     9781 gaacgctatg cctccgcgct tacccgcgaa tctgtcgcac aggcattcta tatttcacca
     9841 aactatctct cgcacctgtt tcaaaaaacg ggggccattg gttttaacga atacctgaat
     9901 cacacgcgac tggagcacgc taagacgtta ttgaaaggct atgacctgaa ggtaaaagaa
     9961 gtggcgcacg cctgcggctt tgtcgacagc aactacttct gccgtctgtt tcgtaaaaac
    10021 accgaacact cgccgtcgga atatcgccgg cagtaccaca gccaactgac agaaaaaccg
    10081 actactccag aataagatgg gtttgctgcg gcgcagaaag cactttgcgc accgcgctca
    10141 ttacccgctg cggctcacgc aggaatgcgt agatgctgca ttgcacatag cggcaatgtt
    10201 caaatcgctc ggcaccggca agctcgatat ccgtaatcaa cagagcaaca tccgcccgac
    10261 ggatatcctc gtccgctaaa cgattctccg ttcccagcgc gccctgagtt tcaatgctga
    10321 cgccccactt ctctaactgg cacaactttt ccagccgttc cgccgccata taagtatgcg
    10381 ccacgccgct tacgcaggcg gttactgcca ccaggtatgc cattttaacc tcccacggta
    10441 acctgaaatc cggcccgttc tgccatttcg cgcatcgttg tcacatcggc tgacgacggc
    10501 gcaggcacct ctttcatcga ccatgttttc cccagcaggc ggtatttcgg ttcgccgtac
    10561 tgatgaaacg gtaacagatg gatctgcctg attttcagcg ggatcagtac atccagcgcc
    10621 tgctgcatat tctcccggct gagcgtgaaa ccagggatca acggtaaacg cgggatcacg
    10681 ttgacaccct cactcaccag caaacgcaga ttctccagca cgcgtggcag gttcatcttc
    10741 accacatccc gcgcctgagt cgcgtccata atttttaaat cgaacagcac ttcatcgcac
    10801 aatttcgcca gcggtaacag cttgcttgct ggcgcgtctc cggcagtttc aatggcgcat
    10861 gagacacccc acagccgcag tcgctgtaaa aaacgggtag caaactccgc ctgcattaac
    10921 acttcgccgc cagaaagcgt gacgccgccg ccggacgtgc gaaagaaaat gtcatctttc
    10981 atcacttccc gttccagagc gtcaaggctg atatcgcgac caatccgttc aaacgccccg
    11041 gaggggcatt catccgcatc acgcaaacat ttcgcacagt gcagacattt cgcctctctg
    11101 cgtaccgtct ggattttgcc ggagatcgac tccggattag cacaccacgg gcaaagatgc
    11161 ggacagcctt taaaaaagac caccgtacga atgccctcgc cgtcattcag tgaataacgc
    11221 tgaatgttga aaatgcgcgc cacatcatcg cggcgcgttt ccacaacgtt acagctgatg
    11281 cgctgtccgg cggatgatgt catcctggat ctccttcgac agttcgacaa agaaggcgct
    11341 gtatccggca acgcgcacca ccagcccggc ataatcttgt gggcgctgtt gcgcttcccg
    11401 caacgtgtcg gcgttcacca cgttaaactg aatatgctgc aacttaagct gggtaaacac
    11461 ccgtaagaag tcggccagtt tgcgtaatcc cgcttcacct tccagggtcg ccggagtgaa
    11521 tttcacgttc agcaacgtac cgttagacag cagcgtatta tcgagcttac tgactgactt
    11581 cagtaccgcc gttggccctt gtgcgtcctg acccagcata ggtgacaagc cgccgtctgc
    11641 cagttgttcc ccggcaaaac gaccgtctgg cgtcgcgcca accaccgatc ccaacggaac
    11701 gtgagcagaa acggtatacg atcccggcgt gaagtaaccg ccgcgcgggt tctggtattt
    11761 ttccacttct ttgcagtagt ggcgcaacag ttcggcgcta atgttatcca cctcgtcgat
    11821 atcgttaccg tatttctcaa agcggttaat taagcgagcg cggacttttt cgccttctgg
    11881 cgttgcaaag ttggctttta ataccgacag caattcgtca aaacttaaac gctgttgctc
    11941 aaaaaccatc cctttcaacg catggagaga atcgctcagg ttggcgatac caattccctg
    12001 tacgccggag aagttataac gcgcgccgcc atcggtaata tcgcgacctt tttccagaca
    12061 atcgctgata aaagacgaga gcagcggtac aggtgcccag tcgcgatggc cgatatcaca
    12121 aatattgctg ccctcaacca tcagggtgat gtagtggctg atcttggcgc ggatctgctc
    12181 cagtaaacct tcataagtca gcgcagcatt gccttcattt tcatgcaggc agatttccat
    12241 cactttcagc agattaaaca tcgcgatgtc atgcaagccg taggttctgc cgggaataga
    12301 taattccaca cagcccacta cggaatagtc gcgcgcatct tccagcgaaa cgccacggtt
    12361 gaggaacgct ggcaccacca cttcatcgtt aaagatttgc ggaataccgg tgccgaggcg
    12421 aatggtttcg gcggttttca tcaggaacgg cgtgtcgatc aaggcgttag tgcgcacgcc
    12481 gaggttcggt tgcggtaatt gcacgctttg ataggcgtca aggcaaagga acgaaagcac
    12541 gttcaccgcg ctacgtccgt tctcggttaa cccgccgagc agtgcggtat agccggtcgg
    12601 gaaaccggcg aaataacgcg cgctactggt ggagcgcaac agcacgatgt cgttgcattt
    12661 cacccataaa gattcgagca gttctttcag gaacgccgga tcttcgccct gggttaaaga
    12721 tgcctgatag aacggcaaca tatactggtc gaagcgccct aaagacagcg aactggcgtt
    12781 ggattcgtat tgcagaatga tgttcatgta ccagaataac tggcacgcct gccagaacgt
    12841 ctgcggctta tgttgcgcgt tatggcggga gatctccgca atagtcagca gctcttcgcg
    12901 acgctgggca tctgtgcagt ttgccgccat cgtttccgcc agttcggcgt aacgcaaaat
    12961 gtgtttctgc gaggcttcca gcagtaacaa cgcggcctga taaaagtgat tctccggctg
    13021 ttgctgacaa tgttgctgca tctgtgccac cagctcaccc agaccgtgat tcaacaggcg
    13081 tgggtaatca ataataatgt gcccctggcc tttatccgtc tggttgatgc tgaaaatctg
    13141 cgtgttggtc gcggctttta cttcatctgt catctgcccg ttgatgaaat ctttcatcga
    13201 acgtttttcc cagtacggga acaactcttc acggtagata cgtttatctt cttcgctgat
    13261 agcaaagcgg tcctgcggac gcgtcgggaa ttgatccagc tctttcagca gccagtaagg
    13321 gtccatttcc ggcgacataa tcccggcgcg cggttttacg gtgcggttac cggcaatcag
    13381 ttcttcatca cgaatcgaaa tttcaacatg ttcaaggata tacgctgttg ctttcgcccg
    13441 gcgcaatatc accggttcgc cttcggtttg ccgatggctg gcggtataaa gcagcgcccg
    13501 ctccagcgag atttcacggg tattggcaaa cagtgcagtt ttgaggcgag agatacgatt
    13561 cgtcatgagg aactcctgtg ggaaagcgaa cccgcccttg cgagcgggag aagaacttaa
    13621 gcggtttgcg ccaggtgagc ttcaattttg ctcataatgg cgtcagcacg ttttactgca
    13681 tcgctgatgt tgacgcgcac gatggtcttg ccagcaaaac gctcttcaaa cttgatgccg
    13741 atatctttgg tcagaatcac catatcagcg ctcgccacgt cttccgcagt cagttcattt
    13801 tccagaccaa ttgacccctg ggtttctact ttcacttccc agcctttcgc tttcgcggca
    13861 ctttccagcg cctctgctgc catataggta tgggcaacac ctgaaggaca tgcggttact
    13921 gcaataattt tcgtcatggc tcgttcctca attaattaat ttcaaaatcc aaatccaggt
    13981 cgtcttcttt ttcatcagtc gaagacccat ttttacgcgc cagacttttc agcacgttca
    14041 cacaaacagc cgtcaccact gctccgacag ccactgctgc gatataaccc aacttacctt
    14101 caaccaccgg cagcacaatc agaccacccc agcctgcata gcactgcgca ccgaccagcg
    14161 ccgcagttac tgcaccacaa actgaaccga ccataatgga aggaatgaca cgcagcggat
    14221 cggcagcggc gaaaggaatc gccccttcag taacgccaac acagcccatc accagtgccg
    14281 ctttgccggt ttcgcgctct tctgcggaga aatttttacg accaatcagc gtcgccagcc
    14341 ccatccccag cggcgggata caaataccca cggcagcaat ggccaccacg gtataaacac
    14401 cctgagcaac gcaaatcagc atgaaggcat aggccacttt gttaaccgga ccgcccatat
    14461 cgaacgccag catcagcccc atgatcaccg ccagcataac aatgctgccc tgctgcatcc
    14521 cctgaagcca ctgagtcagg ctgttggtca acgcccctac aggctcgccc aagccccaca
    14581 tcatgatgcc tgcggtaatc agtgtgccga tgataggaat aatgaagatg ggcatcaccg
    14641 agcgcagaac tttatgcacc ggaattttct tcaggtaatg caccacgatg ccgccgataa
    14701 tcccggcgat cagcgcacca aagaagcccg caccaaagct gttaccaacc caggcaccga
    14761 tagcgcacgg agccagcgca gaacgctctg caatggagta accaatgtaa gcggcgagga
    14821 aaggcaccat cagcgtcaaa cctgcaacgc cgatatcaaa cagttttttc aaattcggat
    14881 cggctacggc atccggcact gcgcctttgc catacaacat gacggaaacc gccagcaaaa
    14941 taccgcccga taccacgaag ggaatcatgt gtgaaacgcc cgtcattaaa tgctgacggg
    15001 tatttttcag gatctgcacc aactcattca taagctgctc ccgggcttgc aaaagcccat
    15061 gtccatattt ttgatggagg aaacaatagg caatcgcagg cggagcaaac agtggataaa
    15121 aaggccattt actggacttt tgtaatatca gtacaaaaat gcgatccgcc tcataacttg
    15181 cgataatccg gaagcatgcc gcaaaacggc gtcttgtgag acgcgtcgca aattgacggt
    15241 tacattacat ttgtccagct tttggcagat ttgtcacatg gcaggccgtt accgtgcgct
    15301 cttatcctgt aaacagaaaa ccgtaccggg agaaaggcaa tggccctgat tgtggaattt
    15361 atttgtgagc tacctaacgg cgtacatgcg cgtccggcaa gccacgttga aacgctgtgt
    15421 aatacttttt catctcaaat tgagtggcat aacctgcgta ctgaccgtaa aggcaacgcc
    15481 aaaagcgctc tggcgctgat tggcaccgat acgctggtgg gtgataactg ccagctactg
    15541 atttccgggg ccgacgaaca ggaagggcac cagcgtttaa gccaatggct gcgcgatgaa
    15601 ttcccccact gcgatgcgcc gctggcggaa gttaaatctg acgaactgga accactgccg
    15661 gtttcactga ccaatctgaa tccgcaaatt atccgcgccc gcaccgtgtg cagcggtagt
    15721 gcaggcggca ttctgacgcc gatctcttct ttagatctca atgcgctgag taatcttccc
    15781 gcagccaaag acgttgacgc cgagcaatcc gcactggaaa acggcctgac gctggtactg
    15841 aaaaacattg agtttcgtct gctggatagc gacggtgcta ccagcgcgat tctggaagcg
    15901 caccgatccc tggctggcga tacttccctg cacgaacatt tactggcagg cgtcagcgcc
    15961 ggattaagct gcgccgaagc gattgtcgcc agtgcgaatc acttttgcga agagtttgcc
    16021 cgttccagca gcagatacct gcaagaacgt gccctggacg tacgcgacgt ctgcttccag
    16081 ttactccagc aaatctacgg tgagcaacgc ttcccggcac cgggcaaact gacgcagtcc
    16141 gccatttgta tggctgatga actgaccccc agccagttcc tcgaactgga taaaaatcac
    16201 ctcaaaggat tgttactgaa aagcggcggc accacctcac atacggtgat ccttgcccgt
    16261 tcgttcaaca ttccgacgct ggttggtgtg gatattgatg ccctcactcc gtggcagcat
    16321 caaacgattt atatcgacgg caacgccggg gcgattgtgg ttgagccagg agaagccgta
    16381 gctcgttatt atcagcaaga agcccgcgta caggacgccc tgcgtgagca acagcgtgtc
    16441 tagctgaccc aacaagcccg taccgctgac ggtatccgca ttgaaattgc cgctaacatc
    16501 gctcactccg tggaagcgca ggccgcattc ggcaatggtg cggaaggcgt tggtttgttc
    16561 cgcactgaaa tgctctatat ggatcgcacc agtgcaccgg gcgaaagcga gctgtacaac
    16621 attttttgtc aggcgctgga gtctgccaac ggacgcagca ttattgtgcg cactatggac
    16681 attggcggcg acaaacccgt tgattatcta aacattcccg cagaggcaaa cccgttcctc
    16741 ggttatcgcg ccgtgcgtat ttatgaagaa tacgcgtcgt tatttaccac acaactacgc
    16801 tcgatcctgc gcgcctccgc tcacggcagc ctgaaaatca tgatcccgat gatctcctca
    16861 atggaagaga tcttatgggt gaaagaaaaa ctggcagaag ccaaacagca actacgtaac
    16921 gaacacattc cgtttgatga gaagatccag ctcggcatta tgctggaagt gccgtcggtg
    16981 atgttcatca tcgatcaatg ctgcgaagag attgatttct ttagtattgg tagtaatgac
    17041 ctgacgcaat atctgctggc agtggatcgc gataacgcta aggttactcg tcactacaac
    17101 agcctgaatc cggcattctt gcgggcgctc gattacgacg tgcaggcggt gcatcgccag
    17161 ggtaaatgga ttggtctgtg cggtgagctg ggagcgaaag gttccgtgct gccgttgctg
    17221 gtcggcttag ggctggatga actcagcatg agcgcaccat caattccggc ggcgaaagcg
    17281 cggatggcgc aacttgatag ccgtgagtgc cgcaagttgc tcaaccaggc aatggcctgc
    17341 cgtacttcgc tggaagtgga acacctgctg gcgcaattcc gcatgaccca acaggacgca
    17401 ccgctggtca ccgccgagtg catcacgctg gaaagtgact ggcgcagcaa agaagaagtg
    17461 ctcaaaggca tgaccgataa cctgctgctg gcgggccgct gccgctatcc gcgtaaactg
    17521 gaagccgact tatgggcgcg cgaggccgtt ttctctaccg gtctgggctt tagttttgcc
    17581 attccacaca gcaaatcaga acacattgag caatccacca tcagcgtggc gcgtctgcaa
    17641 gcgccggtgc gctggggcga tgatgaagcg caattcatca ttatgttaac cctgaacaaa
    17701 cacgctgcgg gcgatcagca catgcgcatt ttctcgcgcc tcgctcgccg catcatgcac
    17761 gaagaattcc gtaacgcgct ggttaacgcc gcctctgccg acgctatcgc cagcctgctg
    17821 caacatgaac tggaactgta aaaggaaaca tcatggaact gtatctggac accgctaacg
    17881 tcgcagaagt cgaacgtctg gcacgcatat tcccgattgc cggggtgaca actaacccga
    17941 gcattatcgc tgccagcaag gagtccatct gggaagtgct gccgcgtctg caaaaagcga
    18001 ttggtgatga gggcattctg tttgctcaga ccatgagccg cgacgcgcag gggatggtgg
    18061 aagaagcgaa gcgcctgcgc gacgctattc cgggtattgt ggtgaaaatc ccggtgactt
    18121 ccgaaggtct ggcagcaatt aaaatactga aaaaataggg tattactaca cttggcaccg
    18181 ctgtatatag cgccgcagaa gggttattag ccgcactggc aggggcaaaa tacgttgctc
    18241 cgtatgttaa ccgcgtagat gcccagggcg gggacggcat tcgtacggtt caggagctgc
    18301 aaacgctgtt agaaatgcac gcgccagaaa gcatggtgct ggcagccagc tttaaaacgc
    18361 cgcgtcaggc gctggactgt ttactggcag gatgtgaatc catcaccctg cccttagatg
    18421 tagcgcaaca aatgctcaac acccctgcgg tagagtcagc tatagagaag ttcgaacacg
    18481 actggaatgc cgcatttggc actactcatc tctaaaggag caattatgga ccgcattatt
    18541 caatcaccgg gtaaatacat ccagggcgct gatgtgatta atcgtctggg cgaatacctg
    18601 aagccgctgg cagaacgctg gttagtggtg ggtgacaaat ttgttttagt ttttgctcaa
    18661 tccactgtcg agaaaagctt taaagatgct ggactggtag tagaaattac gccgtttggc
    18721 ggtgaatgtt cgcaaaatga gatcgaccgt ctgcgtggca tcgcggagac tgcgcagtgt
    18781 ggcgcaattc tcggtatcgg tggcggaaaa accctcgata ctgccaaagc actggcacat
    18841 ttcatgggtg ttccggtagc gatcgcaccg actatcgcct cgaccgatgc accgtgcagc
    18901 gcattgtctg ttatctacac cgatgagggt gagtttgacc gctatctgct gttgccaaat
    18961 aacccgaata tggtcattgt cgacaccaaa atcgtcgcgg gcgcacctgc acgtctgtta
    19021 gcagcgggta tcggcgatgc gctggcaacc tggtttgaag cgcgtgcttg ctctcgtagc
    19081 ggcgcgacca ccatggcggg cggcaagtgc acccaggcgg cgctggcact ggctgaactg
    19141 tgctacaaca ccctgctgga agaaggcgaa aaagcgatgc ttgccgccga acagcatgta
    19201 gtaaccccgg cgctggagcg cgtgattgaa gcgaacacct atttgagcgg cgttggtttt
    19261 gaaagcggcg gctggcggcg gctcacgcgg tgcataacgg cctgaccgct atcccggacg
    19321 cgcatcacta ttatcacggt gaaaagtggc atttggtacg ctgacgcagc tggtactgga
    19381 aaacgcgccg gtggaggaaa tcgaaaccgt agctgcactt agccatgcgg taggtttgcc
    19441 aataactctc gctcaactgg atattaaaga agatgtcccg gcgaaaatgc gcattgttgc
    19501 aaaagcatct tgtgcagaag gtgaaaccat ccacaacatg cctggcggcg cgacgccaga
    19561 tcaggtttac gccgcactgc tggtagctga ccagtacggt cagcgtttcc tgcaagagtg
    19621 ggaataacct actccaaact cccggcttgt ccgggagttt gaacgcaaaa ttgcctgatg
    19681 cgctacgctt atcaggccta cgcaatctct gcaatatatt gaatttgcgt gcttttgtag
    19741 gccggataag gcgttcactc cgcatccggc actctgtgcc agtaatctga aactcccacg
    19801 ttgttcagga gttttttatc cgcttctgga cattttctcc acagaacaat cgttagcccg
    19861 gaaggtcgaa tctgtgactc ttattttcac ggacgaacca gatgaaagct tcactggcgc
    19921 tgctcagcct gctaacggca tttaccagcc actccttaaa atctcccgcc gttccaccaa
    19981 ccgtggtaca aatccaggcc aataccaacc ttgccatcgc agacggtgcc agacagcaaa
    20041 ttggcagcac gctattttac gatcgcagct tacctatcct ggcggtgatg ttccgcaaga
    20101 acgcggtgta tgttccgata tggtgatccg cgcattgcgc agccagaagg tcgatttgca
    20161 gaaactggtt cacgaagata tggcgaagaa ttttgctgag tacccgcaaa aatggaagtt
    20221 aaagcgcccg gacagcaaca tcgatcaccg tcgggtgcct aatctggaaa cctggtttag
    20281 ccgccacaac aaaacacgcc ccatcagtaa aaatgccggt gactatcaag cgggcgatat
    20341 tgtctcctgg cgattagata acgggctggc gcatattggg gtggcgtcag atggcttcgc
    20401 ccgcgacggt acgccgctgg tgatacataa cattggcgca ggcgcgcagg aagaagatgt
    20461 gctgtttagc tggcggatgg tggggcatta tcgttatttt gttaaataag agatcgcccc
    20521 acatgaagaa aaaattcaga tgctttttac actgacagct tcttttttct tacggctcac
    20581 cagcgccagt gccagcacaa tcagcacaat accgctacct tcaacggccc ccggattctc
    20641 accgagcagc caccaggaaa agagtacgcc acaaacggga acggccagtg tgcttaagct
    20701 ggcaatactg gcaggcaagt ttttcagcac aaacagccat aagctccacg ctagcgccgt
    20761 cgccagaatc gcactgtagg ccagcgccca gaacacggtg ggctgccagt caatttcacg
    20821 ttgcggcacc agtaaagcga ccacactcat caccagcgcc gcatacagca tctgccagga
    20881 tgttaacgat aataaatcca cgcgcggatg acgggcgtat aggcgtttag caacaatcgc
    20941 gctcgccccc caactaacac cggagaggat tgccagcatg gcacttttca tcgaagagaa
    21001 atcgagttgc cacggctgca acaccaaaaa taagccgaaa gcggcaatca gaatcgcgaa
    21061 atattgccca cgtcgcaggc gttcaccgag aaacaacgcg gcgaaaatca ccacccagaa
    21121 cggcatggta tagctcagga tcgccacctt ccccgcacct ccgctgacca acgcccactg
    21181 cgccagacca accatcccgc aggtttgtaa cagggcaatg gctaaggtgt atttaaacgg
    21241 cgtcgggcgc attccgcgac cacgtaataa aaggacgatg aataaaacga gagcgccgaa
    21301 aatgcagcgt aaggcggtaa agtcgaaggc accgatgtaa ctggtgactt gcttcatgaa
    21361 aatccagcta taactccaga taagtgtgag cacaaccaag ccactgattg ccagtgggtt
    21421 gctctttcct gcggcagaca tgagagatcc agtgtgtagt tttcctgtgg acactatacg
    21481 ccggtaaaga aacttcagcg actgcctgaa agacagtcgc tgaagaaggt cagattacag
    21541 caggtcgaaa cggtcgaggt tcatcacttt cacccatgcc gctacgaagt ctttaacaaa
    21601 cttctcgtgg gcatcgctgc tggcgtaaac ttccgctact gcacgcagga cagagttaga
    21661 accaaatacc agatccgcac ggctggctgt gtatttcact tcgccggttt cgcggtcacg
    21721 tccttcgaac agctctttcg attcgtcagt cgctttccac tcgtaacgca tatccagcaa
    21781 gttcacgaag aagtcattgc tcaatacgcc aacgcggtca gtgaagacgc cgtttttgct
    21841 gccatcgaag ttggcaccca gtacacgcat cccgcccacc agcgcagtca tttccggcgc
    21901 ggtcagcgtc agttgctgtg ctttgtcgat cagcagtgac tcggtggtgg aaacgtccag
    21961 acgagcgcga tagttacgga aaccgtcagc aattggctcc agcagctcaa acatctcaat
    22021 gtcagtctga tcctgacgcg catcaacgcg acccggcgca aacggtacat gaatgctcaa
    22081 acctgcggcg cttgcggctt tctcaacacc aaccacacca gccagcacga tgatatccgc
    22141 cagcgaggct ttaccagact ctttctggat tttctccaga acaggcagag cacgaacggc
    22201 tgcggcgttc acatcccagt cgcgctgcgg cattaatgcc agacgcgcac cgttggcacc
    22261 accgcgtttg tcgccaccac ggaaggtaga agcagatgcc caggccaccg ataccagctc
    22321 actaacagac agaccagaat ccgcaatcgc gaatttcaga tcgataatgt cctgctcggt
    22381 cgggttgtag atcggctgcg gcagcggatc ttgccagatc agatcttctt tcggcacttc
    22441 cggcccgatg tagcgagatt tcggccccat atccctgtgc gtcagtttga accaggcacg
    22501 ggcaaaggct tcgttgaacg cctgcggatc gttgaggaaa cgacgagaga tcttctcgaa
    22561 ctcaggatca aaacgcagcg tcaggtcggt caccagcatt gtcggtttac gtttcttcga
    22621 cggatcgaac ggatccggga taatttccgg tgcgtctacc gcttcgaact ggattgctcc
    22681 agccggactg cgggtctgta cccactcata cttgaacaga ttctcgaaga aatagttgct
    22741 ccactggatc ggcgtctggg tccagactac ttccagacca gaggtaatgg catctgcgcc
    22801 aacgccgctg ccgtaagtgc tcgcccagcc taaaccttgt tcttcaatcg gtgcagcttc
    22861 tggatcagga cctacgtttg atgtcggacc ggcaccgtgg gttttaccca gcgtatgacc
    22921 acccgcaatc agcgccacgg tttcttcgtc gttcatgccc atgttgccga aggtcgcgcg
    22981 gatagctgct gccgcagaaa gcggttcgcc gctgtgatcc gggccttccg ggttaacgta
    23041 aatcaggccc atctcggttg cacccagcgg tgctttcgcc agcgcttccg gatgacggtg
    23101 agtcagccag gctttttcat caccccagtt aacatccaga tccggttccc agacgtcttc
    23161 acgaccggca ccaaaaccga aggtacggaa gccggagttt tccagcgcca cgttacctgc
    23221 gaggataaac aggtcggccc aggagatttt ctgaccatat ttctgtttga ttggccacaa
    23281 caggcgacgc gctttatcga ggcttacgtt atccggccag gagttcagcg gtgcaaaacg
    23341 ttgctgacca cgacccgcgc cgccgcgtcc atcgattgaa cggtaagtac ctgcgccgtg
    23401 ccaagccata cgaataaaca gaccggcgta gctgccccag tcggctggcc accacggctg
    23461 agattctgtc agcagggctt tcagatcttt tttcaggccg tagtaatcta atttgctgaa
    23521 ttctttgcgg tagtcaaagt cctcacccag cgggttagaa cgattagaat gttggtttaa
    23581 caggtcaaca cggagttgat ttggccacca gtcgcgagtg gttgtgcccg cccccgcgct
    23641 ctggtcgtga ccgccctgat ggaacgggca tttgccagtg gctgtggtgt tatggatatc
    23701 gtctgacgtg ctcatcaatg tgctcccctc tacagtgtta ccgttacgat acacagcgtt
    23761 agagagaagt tataattgaa ttaatccacg gatgcgatag ttgagatctt acatattgtt
    23821 ggttaaagag atgtagatca aattgatcgt aattaagctt ttttgattat gtccgaattc
    23881 ggacaaatgc tttataaaaa agggttttag cttcatatcc ttcaagtaat gagggagaaa
    23941 acaaggctga caaccttatt ttcccgccct catttcgagg cagcattttg tgctctgttt
    24001 aaaatttgtg atcactgtgt gattttcaca aaagccacac tatttataaa ccaggtcgaa
    24061 cacccagcgt atggcaaatc gcgtaactca tttcagcgcg gttaagcgta tagaagtgga
    24121 aatctttcac tccttcacgg cttaaaatct tcaccatatc catggcaata ttcgcgccca
    24181 ccagtttgcg ggtttcggca tcgtcatcca gcccgtcaaa catttgcgcc atccacgccg
    24241 gaatacgcac gttggtcatg tcggcaaatt tcttcgcctg tttaaagtta gataccggca
    24301 aaatgcccgg gatgatttcc acatcaatgc ccgccgatac acagcggtca cgaaaacgca
    24361 ggtagctttc gacatcgaag aagaactgag taatcgcgcg gttggctccg gcatccactt
    24421 tgcgtttcag attaagcaaa tccgcctgag cgctttttgc ttccgggtga acttccggat
    24481 acgccgccac ggatatatcg aaatctgcca cttcttttaa cagcgtcacc aggtcagaag
    24541 catacatttc tggcttacca cttcccggcg gcagatcgcc acgcagtgcc acgatatgac
    24601 gaataccgtt attccagtag tcgcgtgcaa tggtgcgcag ctcgtcgggc gtcgcatcaa
    24661 tgcaagtaag atgcggtgcc gcttccagac cagtgcgatc tttaatgcct ttaataatgc
    24721 tgtgcgtacg gtcgcgctcg ccggagttcg cgccataggt caccgataca aacttcggtt
    24781 tcaggctgct aaggcgatcg atggagttcc acagggtctg ctccatttca ctggtacgcg
    24841 gcgggaaaaa ctcgaacgaa acgttaatct gcccctggac ttctgccagg ctctgattca
    24901 gggcatcccg ctggctggcg tgaaaaaagc tcatacctta cctcatcaat cgcttgtagt
    24961 tatatgttgt gttgtgcaca tctatccatt tagacgtcca gatgtaaaga tgaaggaaaa
    25021 gccgaagggc gtcaaccgaa aaatgaccaa tgatggtgag gaaaaatcag ggaagatgaa
    25081 aaaacttcag gatggtaaga aaaagaaaat ccgatatgca aagcaaaccg gactgcaaat
    25141 ataaactaac catgtactta ttgcttcttg ccggattcgt cgtaaacgct tcatccggcc
    25201 taattctatg ataattcaat atatttcatt agaaaccata gacccgaaaa catgagtacc
    25261 gggcattatt aaatttctga aattacaaca actgtgccag ccggttgata tccgactgaa
    25321 tcgccccggc ggtgacgtcg cgcccagcgc caggcccacg gatcaccagc ggattatcgc
    25381 ggtaccagcg gctttcgatg gcaaagacgt tatcgcatgg cagcagtgat gccagcggat
    25441 agtcttcacg caccgcctcc acgcccacac gcgctttgcc gttggcatcg aaacgcgcca
    25501 cgtagcgcag caccagcccc atttcgcggg ccgcttccag ccgttgcacc atctgctcgt
    25561 taagttcatc gccattttca aagaaatggt cgatgctgcc gccttcgcaa tgagcaggca
    25621 ccagcgattc cacacgtacc tggtccggtt caatgttgta acccgcttca cgcgccagaa
    25681 tcaccagctt gcgcattacg tctttgccgg agaggtcgtc acgcggatca ggctcggtta
    25741 agccctgctg ccacgcctga tccaccagct cggtaaacgg cacgctaccg tcgaattgca
    25801 ggaacagcca ggagagcgtg ccggagaaga tcccactgat cgacaaaatg gtatcgccgc
    25861 tgtcgatcag atcgcgcacg gtatggttga tcggcaagcc cgcaccaacg gtggcgttgt
    25921 atagccagtg acgcccggtt ttttcgaagg cgtcgtggat ctggcgatat ttattgctgt
    25981 cgcttgctcc cgccagtttg ttggcactga tgacgtggaa accgtggctg gcgaaatcga
    26041 gatactgatc agcaagctgc tggctggcgg taacgtccaa caccactaaa tcatcatacg
    26101 gatgggcgcg catccacagg aaaagcgact cttcatcctg ctcgaccgct tcatcgttga
    26161 agaaggctaa cgcgcggctg gcgtccagcc cgtcataact caacagactg cggcggctgt
    26221 ccaccacgcc tgccagcaca aattcaaagc cggtacgcgc cgaaagcgtg ctctgctcgc
    26281 gggcgaacag ttccagccag cgagagccga tattgccttt gccgaacaat accaggccga
    26341 tgcgtttttc tgcgcggaag acggactgat gcagcccctg aatcaggctt tcggtcgggc
    26401 cggtgcgcag tactgccacc agactgatgc cgtcatcgga ctgccacgta aactcgaccg
    26461 gctggccttt cagttgctgc cagaagcggt ggcaatgcag cgggttacgg gtgacacccg
    26521 ccccgaccat cgccaccagc gccagcccct gacgcaggcg cagttctcca ggtaatcccg
    26581 cttcgtcgag gattttcagc gcactgtcgg ccacttctga ggtgtagcaa aattgcaaca
    26641 actggcgatc gttatgcacg ccaaccgcca gtggacgcac ctgggcacgt ttcaggatct
    26701 ggtcgatctc tttatgcgcc agtttgaaat cctgactggc gggcacctga aactcaatca
    26761 aacagacatc atcgtggctg gtgacaatac gcgcaccagt accggaggcc agcacgcgtt
    26821 caatgcgcgt ggaaccttga tccggcgtgt agctacagcg cagctgcagg tcgatttcgc
    26881 tgccagaaac tggctgtaaa gtacgggcgt gaagaacggg agccgccaga cgcgccagtt
    26941 cgctggcctc atccagacgc agcaacggca gcaggcaggc atctttcact ttacgcgggt
    27001 cggcactgta taccccggcg acgtcgctcc agatggttac acgagaaaca cccgccagcg
    27061 caccgatttg tgtcgcggaa tagtcggaac cgttacgccc cagcaacacc gtttcaccgg
    27121 cgttgttacg gctgataaac ccggtcacca ccagacgttt gctcggatgt tgtaccagta
    27181 gctgttgcag caacgggtaa gaaagccctt catcaacctg cggttgcgca gcgcgttcag
    27241 cgcgtaaaaa ctcacgggca tcaagccagg ccgctggcag cccttgttga ttaagtaccg
    27301 cagacatcag gcgcgccgac cacacttcac cgtggcccac cacttccgca tacaccgcat
    27361 cgttaatacc actgtcgagc aacgccgcca ggcgctcaag atcgctgaca aaagcgctaa
    27421 tgaggctatc ggcttcttca gcgggtagca gaccgctaat cagatcgcac tgatagcgac
    27481 gcagcgtttg ttgaacctga tgcgcagaga gacgatcggt ctggcttagt ttcaaccagt
    27541 taatcaactg gttagtggtg ctaccggcgg cggaaaccac catcatatcg tcaggctgag
    27601 agaactccgc cataatgccc gcgacacgca aataacactt cacatcagcc agactactgc
    27661 caccaaattt atgcagctga cgacctttcg cccctgcctg cgcaatcaca ctcattttta
    27721 ccccttgttt gcagcccgga agccattttc caggtcggca attaaatctt cgccatcttc
    27781 aataccggtg gagatacgca gcagcgtctc ggagatcccg gcggcagcac gcgcttctgg
    27841 tgccatgcct gcatgtgtca tggttgcggc gtgagagatt aaactttcca ctccccctaa
    27901 tgattccgcc agcgtaaaca acgacagccc gcccaggaaa cgacacagcg tctgctcatc
    27961 gccatccagt tcaaaactca acattgcgcc aaagcctttt tgctgacgcg cggcaatttc
    28021 atgcccctga ttttccggca gcgacgggtg atacagtttt ttcaccaacg gctgggtttg
    28081 cagatatttc acaatcgcct gcgcgttgcg ctgcgccagc tccatacgcg gcaccagcgt
    28141 tcgcaacccg cgtagcagca gatagctgtc aaacgcgccg cccgtcacgc caatattgtt
    28201 tgcccaccag gcaagttcag tgacaacgtc cgggtcttta gcaatcacca cgccagccac
    28261 cacgtcagag tgaccgttca gatatttcgt acatgaatgc aacaccagat cggcacccaa
    28321 tgccagcgga ttttgtaatg ccgggcttaa gaaggtgtta tccaccacgc tcaccgcccc
    28381 gacttccctt gccagatggc agattttcgc aatatccacg acgcgtaaca atggattact
    28441 tgggctttct accagtacca gtttgggttt ttctgccagc gctgcccgta atgcctgttc
    28501 atcgccttga tcaacaaaca acacgcgata gcaaccgcgt ttcgccagac tgtcgaacag
    28561 gcgatagcta ccgccgtagc agtcgtgcgg cgcaaccagc agatcgccag gtttcaaaaa
    28621 gacggtcgtt accaggtgaa tcgcggacat gccggtatta gtaagtactg cgccagcacc
    28681 gccttccagt tctgccagcg cacgctgaac cacatcgcgc gttgggttgc cgcgacgcga
    28741 gtaatcatgc gcgcgcggtt cattaaatcc ggtaaagtta taggtgctgg aaagatggat
    28801 gggagggaca acgcaaccat actgttcgtc gtcatttaac ccgctacgca ctgcgatggt
    28861 ggcctgttta cgcgtcatgt gatgaagttt cctgggcttt gtcggtgaaa tgtcaggcac
    28921 cagagtaaac attgtgttaa tggacgtcaa tacatctgga catctaaact tctttgcgta
    28981 tagattgagc aaatcccaaa tagccgttaa aattatatgc attatcacgc cgacaggtgc
    29041 attacacgat gtcacggtaa cgcctgtacg gtaaactatg cgggtttacg gtcagtaccc
    29101 acataaattg tggggtctgg tctcaattta ttgacgaaga ggattaagta tctcatggct
    29161 gaatggagcg gcgaatatat cagcccatac gctgagcacg gcaagaagag tgaacaagtc
    29221 aaaaagatta cggtttccat tcctcttaag gtgttaaaaa tcctcaccga tgaacgcacg
    29281 cgtcgtcagg tgaacaacct gcgtcacgct accaacagcg agctgctgtg cgaagcgttt
    29341 ctgcatgcct ttaccgggca acctttgccg gatgatgccg atctgcgtaa agagcgcagc
    29401 gacgaaatcc cggaagcggc aaaagagatc atgcgtgaga tggggattaa cccggagacg
    29461 tgggaatact aaagcggaaa agagcggcgc ggagtggaat cgcctgatgc actacgctta
    29521 tcaggcctac gtcatattgc aatttattga atttgcacga acttgtaggc cggataaggc
    29581 gttcacgccg catccggcat aaacaacgag cacgttgtct gcgacccacc gctttttata
    29641 catggacgtt taactatgaa aaacaggctg ctgatcctca gcctgctggt ttctgtacct
    29701 gcctttgcct ggcagccgca aactggcgac atcatctttc aaatctctcg ctcatcgcaa
    29761 agtaaagcga tccaactggc aacccattcc gattacagcc acaccggtat gctggtgatg
    29821 cgtaacaaaa agccctacgt ttttgaagcg gtcggcccgg tgaaatacac cccgctcaag
    29881 cagtggattg cccatggtga aaagggcaac tacgttgttc gccgcgttga aggcggactg
    29941 agtgttgaac aacagcaaaa actgacgcaa acggcaaaac gttatctcgg taaaccgtat
    30001 gatttcagct tctcatggag cgacgatcgc cagtactgtt cggaagtggt gtggaaggtt
    30061 taccagaacg cgctgggaat gcgcgtgggc gaacagcaga agttgaaaga gtttgatctc
    30121 agcaacccgc tggtccaggc gaagctcaaa gaacgctacg gtaaaaatat tccgcttgag
    30181 gaaacggtag tttcaccaca agccgttttc gacgcgccac aactcaccac ggttgccaaa
    30241 gagtggccgc tgttttcgtg gtgatacgac caaaattcag gcacaaaaaa agcgccgtgc
    30301 ggcgcttttt tcggaaatcc ggtcttattt gctgcccggg atgttgaaac gcttgttgaa
    30361 gcggtcaaca cggccaccgg tagcaacatc acgctgtttg ccagtgaaga acgggtggca
    30421 cttgctgcac acgtcgaggt tcaggtcatg accaacggtg gagcggattt ttattacgtt
    30481 accgcaagag cagctagcag taatttcttc gtatttcggg tgaatatctt ttttcatggg
    30541 aaaacctcgg tttaaggccg cgtcgctctt ccagccctaa cgccagacac cacgcgatgt
    30601 taaaagtata gcttcaatac gatcatttcg tacgaaggcg cgaaatcata cagaaattaa
    30661 ccagcgtatg caaactgatc cgcactcttc tacggcaatg tgtatactaa cccaccgaat
    30721 ttcaagtcag gatgatgcta tgcccgttgc ccacgttgcc ttgcccgttc cgcttcctcg
    30781 tacctttgac tatctgctgc cagaaggcat gacggttaaa gctgggtgtc gcgtgcgcgt
    30841 gccgtttggc aaacagcagg agcgcatcgg gattgtggta tcagttagcg atgccagcga
    30901 actgccgctc actgagctaa aagcggtagt cgaagtgctg gatggtgagc cggtgtttac
    30961 tcactccgtc tggcgattgc tgctatgggc ggcagattac tatcatcatc cgattggcga
    31021 tgtgctgttt catgccttgc cgattttact acgccagggg cggcctgcgg cgaacgcgcc
    31081 gatgtggtac tggtttgcca ctgaacaagg tcaggcggtg gatctgaaca gcctgaaacg
    31141 ctcacccaag caacaacagg cgctggcagc gttacggcaa ggcaaaatct ggcgcgacca
    31201 ggttgccacg ctcgaattta atgatgccgc gttgcaggcg ctgcgcaaaa aaggtctgtg
    31261 tgatttagca agtgaaacac cagagtttag cgactggcga acgaactatg ccgtttctgg
    31321 tgagcggttg cgattgaata ccgaacaggc caccgccgtt ggcgcaattc atagcgcggc
    31381 agatactttt tctgcctggc tgctggcggg cgttaccggt tccggtaaaa cggaggttta
    31441 tctcagtgta ctggaaaacg tgctcgctca gggcaaacag gcgctggtga tggtgccgga
    31501 aatcggcctg acaccgcaaa ctatcgcccg ttttcgtgaa cggtttaatg cgcccgtgga
    31561 agttctgcat tccggtctga acgacagcga gcgtctttcg gcgtggctga aagcgaaaaa
    31621 tggcgaggcg gcgattgtga tcggcacccg ctccgcgctg tttacgccgt ttaaaaatct
    31681 cggcgtgatt gtcattgacg aagagcacga cagttcctac aagcagcagg aaggctggcg
    31741 ctatcatgcc cgcgacctgg cggtgtatcg tgcgcatagc gagcaaatcc cgattattct
    31801 tggctccgca acgcccgcgc tggaaacgtt gtgcaacgtc cagcagaaaa aataccgcct
    31861 gctgcgcctg acgcgccggg cggggaatgc gcgtccggca attcaacacg tgctggattt
    31921 aaaaggtcag aaggtgcagg caggtctggc tccggcgtta atcacccgta tgcgccagca
    31981 tttacaggcc aacaatcagg ttatcctctt tcttaaccgc cgtggctttg cgcctgcgct
    32041 actgtgccac gactgcggct ggattgccga atgcccacgt tgcgatcact actacacgct
    32101 gcatcaggcg caacagcatt tgcgctgtca ccactgtgac agtcagcgtc cggtgccgcg
    32161 ccagtgccct tcctgcggtt ccacgcacct ggtacccgtg gggctgggca ccgaacagct
    32221 tgaacagacg cttgcgccgc tgttccctga tgtgcccatt tctcgtatcg accgcgatac
    32281 caccagccgc aaaggggcgc tggaacagca actggcggaa gtacatcgcg gcggtgcgag
    32341 gattttgatc ggcacacaaa tgctggcgaa aggtcaccac tttccggatg tgacgctggt
    32401 ggcgttactg gatgtggacg gcgcgctgtt ttctgccgat tttcgctcgg cagagcgttt
    32461 cgctcagctt tacacccagg tcgctggtcg tgccgggcgt gcgggtaaac aaggcgaagt
    32521 ggtgctgcaa acgcaccatc cggaacatcc tctgttgcaa acgttgctct ataaaggcta
    32581 cgatgccttt gccgaacagg cgctggctga gcgacggatg atgcaactgc cgccatggac
    32641 cagccatgtg attgtgcgtg cggaagatca taacaatcag catgcgccgc tgttcctgca
    32701 acaactgcgt aatctgatcc tctccagccc gctggcagac gacaaactgt gggttctcgg
    32761 tccggttccg gcactggcac ctaaacgtgg cggtcgctgg cgctggcaga tattgttgca
    32821 gcacccttcc cgcgtgcgct tgcaacacat cattagtggt acgctggcgc tcatcaatac
    32881 aataccggat tcccgcaagg tgaaatgggt actggatgtt gatccgattg agggttaaac
    32941 cgcttacgat gcgaggcgga tcgaaaaatt caatattcat cacacttttc atgaaaattc
    33001 tgtaaccgtt ttcacgcgct atctgctaaa aatgttgccg atgtgaagta aacatggatg
    33061 tagtacgcct gacgtgccag gcgaggagtg agtgtgaaag cgaagaagca ggaaactgcc
    33121 gcgaccatga aagacgttgc cctcaaggca aaagtctcta cagcgactgt ctcccgagca
    33181 ttaatgaatc ccgataaagt ctcccaggcc acccgtaatc gggttgaaaa agcggcccgg
    33241 gaagtgggtt atttaccgca gcctatgggg cgcaacgtca agcgtaatga atcccgcacc
    33301 attctggtga ttgtcccgga tatctgcgat cccttcttta gcgaaattat tcgcggtatc
    33361 gaagttacgg cggcaaatca cggatatctg gtgctgattg gcgactgtgc gcatcaaaat
    33421 cagcaggaaa aaacctttat cgatttgatc atcaccaagc aaattgatgg catgttgctg
    33481 ctgggttcaa ggctgccgtt tgatgccagc attgaggaac agcgtaatct gccgccgatg
    33541 gtgatggcga acgaatttgc accggaactg gagctgccta ccgttcatat cgacaatctg
    33601 accgccgcat ttgatgcagt aaattattta tatgagcaag ggcataaacg gattggctgt
    33661 atagccggtc ccgaagagat gccactgtgt cactaccgcc tgcaaggcta tgttcaggcg
    33721 ctgcgtcgct gcggaattat ggttgatcca caatacatcg cccgtggcga cttcaccttc
    33781 gaagccggaa gcaaagcaat gcaacagctg cttgatcttc cacaaccgcc tactgcagtc
    33841 ttctgccata gcgatgtgat ggcgctcggc gcactttctc aggcaaaacg ccaggggctg
    33901 aaagtcccgg aagacctttc cataatcggt tttgataaca tcgacctgac gcaattttgt
    33961 gatccgccgc tgacaaccat cgcgcagccg cgttacgaaa tcggtcggga agctatgctg
    34021 ttattgcttg atcaaatgca ggggcaacac gttggcagtg gctctcgttt aatggactgc
    34081 gaacttatca tccggggatc aacacgcgcg ttaccttaaa gtaaaccttt aagactgccg
    34141 tgactggtca aaggcccgcc gcttaagtaa catggcgggc tgacgaacga ataaatacag
    34201 cgaaacgata gtggcacaac gagattatgt acgccgcagc caaccggcac cttcgcggcg
    34261 aaaaaagagc acctcacgga aaaagcaacg aaatctgcct gcggtttctc ccgctatggt
    34321 cgctattgct gccgccgttc ttgtgacctt tatcggtggt ctgtacttca ttacgcatca
    34381 caagaaagaa gagtccgaga cgctgcaaag ccagaaagtg accggaaacg gactaccacc
    34441 aaaaccagaa gaacgctggc gctacattaa agagctggaa agtcgccagc cgggtgtgcg
    34501 tgcgcccaca gaaccttctg ccggtggtga agtgaaaacg ccggagcaac tgacaccaga
    34561 acaacgtcag cttcttgaac aaatgcaggc tgatatgcgc cagcagccaa cgcagctggt
    34621 tgaggttccg tggaatgaac agacgccgga acagcgtcag caaacgctac aacgccagcg
    34681 tcaggcgcag caattagcgg agcagcaacg cctggctcag cagtcgcgta cgactgaaca
    34741 aagctggcag cagcagacgc gtacgtcgca agccgcgccg gtacaggcgc aaccgcgcca
    34801 gtctaaacca gcttctactc agcagccgta ccaggatctg ctgcaaaccc ctgcgcacac
    34861 gaccgcgcag tcgaagccgc agcaagctgc gccggttgct cgtgccgctg acgcgccaaa
    34921 accgacggcg gagaaaaaag acgaacgccg ctggatggtg cagtgcggtt cgttcagagg
    34981 cgcggaacag gcagagacgg tacgtgctca gctggcgttc gaaggatttg actcgaaaat
    35041 caccaccaac aatggctgga atcgtgtggt cattggcccg gtgaaaggca aagagaacgc
    35101 agacagcacc ctcaatcggt tgaagatggc gggtcataca aactgcattc ggctcgccgc
    35161 cgggggttga aaccctcaaa atccccccca tctataattg cattatgccc cgtacttttg
    35221 tacggggttt gtactctgta ttcgtaacca aggggtcagc tcgtgacaac tatagtaagc
    35281 gtacgccgta acggccatgt ggtcatcgct ggtgatggtc aggccacgtt gggcaatacc
    35341 gtaatgaaag gcaacgtgaa aaaggtccgc cgtctgtaca acgacaaagt catcgcgggc
    35401 tttgcgggcg gtactgcgga tgcttttacg ctgttcgaac tgtttgaacg taaactggaa
    35461 atgcatcagg gccatctggt taaagccgcc gttgagctgg caaaagactg gcgtaccgat
    35521 cgcatgctgc gcaaacttga agcactgctg gcagtcgcgg atgaaactgc atcgcttatc
    35581 atcaccggta acggtgacgt ggtgcagcca gaaaacgatc ttattgctat cggctccggc
    35641 ggcccttacg cccaggctgc ggcgcgcgcg ctgttagaaa acactgaact tagcgcccgt
    35701 gaaattgctg aaaaggcgtt ggatattgca ggcgacattt gcatctatac caaccatttc
    35761 cacaccatcg aagaattaag ctacaaagcg taaggatctc ccatgtctga aatgacccca
    35821 cgcgaaatcg tcagcgaact ggataagcac atcatcggcc aggacaacgc caagcgttct
    35881 gtggcgattg ctctgcgtaa ccgctggcgt cgcatgcagc tcaacgaaga gctgcgccat
    35941 gaagtgaccc cgaaaaatat cctgatgatc ggcccgaccg gtgtcggtaa aactgaaatc
    36001 gcccgtcgtc tggctaagct ggcgaatgcg ccgttcatca aagttgaagc gaccaaattt
    36061 accgaagtgg gctacgtcgg taaggaagtt gattctatta ttcgcgatct gaccgatgcc
    36121 gccgtgaaaa tggtacgcgt ccaggctatc gagaaaaacc gttatcgcgc tgaagaactg
    36181 gcagaagaac gtattctcga cgtgctgatc ccacctgcta aaaacaactg gggacagact
    36241 gaacagcagc aggaaccttc cgctgctcgt caggcattcc gcaaaaaact gcgtgaaggc
    36301 cagcttgatg acaaagaaat cgagatcgat cttgccgcag caccgatggg cgttgaaatt
    36361 atggctcctc cgggcatgga agagatgacc agccagctgc agtccatgtt ccagaacctg
    36421 ggcggccaga agcaaaaagc gcgtaagctg aaaatcaaag acgccatgaa gctgctgatt
    36481 gaagaagaag cggcgaaact ggtgaacccg gaagagctga agcaagacgc tatcgacgct
    36541 gttgagcagc acgggatcgt gtttatcgac gaaatcgaca aaatctgtaa gcgcggcgag
    36601 tcttccggtc cggatgtttc tcgtgaaggc gttcagcgtg acctgctgcc gctggtagaa
    36661 ggttgcaccg tttccaccaa acacgggatg gtcaaaactg accacattct gtttatcgct
    36721 tctggcgcgt tccagattgc gaaaccgtct gacctgatcc cggaactgca aggtcggctg
    36781 ccaatccgcg ttgaactgca ggcactgacc accagcgact tcgagcgtat tctgaccgag
    36841 ccgaatgcct ctatcaccgt gcagtacaaa gcactgatgg cgactgaagg cgtaaatatc
    36901 gagtttaccg actccggtat taaacgcatc gcggaagcgg catggcaggt gaacgaatct
    36961 accgaaaaca tcggtgctcg tcgtttacac actgttctgg agcgtttaat ggaagagatt
    37021 tcctacgacg ccagcgattt aagcggtcaa aatatcacta ttgacgcaga ttatgtgagc
    37081 aaacatctgg atgcgttggt ggcagatgaa gatctgagcc gttttatcct ataatcgcgt
    37141 tcaatcattt tcatcattgt ttgatggggc tgaaaggccc catttttatt ggcgcgtatt
    37201 atgactgaac aacaaattag ccgaactcag gcgtggctgg aaagtttacg acctaaaacc
    37261 ctccccctcg cctttgctgc aattatcgtc gggacggcgc tggcatggtg gcaaggtcac
    37321 ttcgatccgc tggtcgccct gctggcacta attaccgccg ggctattaca gatcctttct
    37381 aacctcgcca atgattacgg cgatgcggta aaaggcagcg ataaacctga ccgcattggg
    37441 ccgctacgcg gcatgcaaaa aggggtcatt acccagcaaa agatgaaacg ggcgctcatt
    37501 attaccgtcg tgctcatctg tctctccggg ctggcactgg ttgcagtggc atgccatacg
    37561 ctggccgatt ttgtcggttt cctgattctt ggcgggttgt cgatcattgc cgctatcacc
    37621 tacaccgtgg gcaatcgtcc ttatggttat atcggtctgg gtgatatttc cgtactggtt
    37681 ttctttggct ggttgagtgt catggggagc tggtatttac aggctcatac attgattccg
    37741 gcactgatcc ttccggcgac cgcatgcggc ctgctggcaa cggcagtact gaatattaat
    37801 aacctgcgtg atatcaatag cgaccgcgaa aatggcaaaa acacgctggt ggtgcgctta
    37861 ggtgaagtga acgcgcgtcg ttatcatgcc tgcctgctga tgggctcgct ggtgtgtctg
    37921 gcgctgttta atctcttttc gctgcatagc ctgtggggct ggctgttcct gctggcggca
    37981 ccattactgg tgaagcaagc ccgttatgtg atgcgggaaa tggacccggt ggcgatgcga
    38041 ccaatgctgg aacgtactgt caagggagcg ttactgacta acctgctgtt tgttttaggg
    38101 atattcctaa gccagtgggc agcataactg acaaatatca attaacaatt gatgattttg
    38161 ccaacagccc acatagcgcg atatactgaa aattctcgca gcaactgaat gttaagccta
    38221 tgaaatacga tacttccgag ctttgtgaca tctatcaaga agatgttaac gtcgtggaac
    38281 cgctgttctc caactttggc ggacgggcgt cgtttggcgg acaaataatc acggtaaaat
    38341 gtttcgagga caacgggttg ctgtacgatc tgctcgaaca gaatggccgt ggtcgtgttc
    38401 ttgtcgtcga tggcggtggt tctgttcgtc gcgcactggt cgatgctgaa ctggcgcgtc
    38461 tggcagtaca aaatgaatgg gaaggtctgg tcatttacgg cgcggtgcgt caggtagatg
    38521 acctggaaga gttggatatc ggcatccagg cgatggcggc aattccggtt ggtgccgctg
    38581 gcgaaggcat tggcgaaagc gatgtccgcg tcaattttgg cggtgtcacc ttcttctccg
    38641 gcgaccatct ttatgccgac aataccggga ttattctttc agaagatccg ctggatattg
    38701 aatgataaga aaggcaccgc aaggtgcctt ttttctgcgt tacctgttgg cctacacagt
    38761 aaagaaatta cgcgaaagat gaagcgtaat cagacctctt ccatgcgacc cagcagggcc
    38821 tgcagacgtt cctgccagcc gttctgctgt tctttcagat ggttgttctc acgctccagc
    38881 tcttcgcgct gatgctgggc attttgaact tcctgcgaca gtgagttgtt tttttctttc
    38941 agctcttcga tttccatctg caacagagtg atggtatcaa tcgcctgctg tacttttgct
    39001 tccagtttct caaacacttc taatgacatt gtcatacctc tcctgaattg caaggcgttg
    39061 atggataaaa aatcctcgtc ccgattaccg gtgacgcctt aataaatacg agcgcacttt
    39121 agttagctcc gattgtatga agccgcgcca tcgctgtcca gcggcacgcc ttgcagatta
    39181 cggtttgcca cacttttcat ccttctccta gtgacataat ccacatcaat cgaaaatgtt
    39241 aataaatttg ttgcgcgaat gatctaacaa acatgcatca tgtacaatca gatggaataa
    39301 atggcgcgat aacgctcatt ttatgacgaa gcacacacat tttaagttcg atatttctcg
    39361 tttttgctcg ttaacgataa gttaacagca tgcctacaag catcgtggag gtccgtgact
    39421 ttcacgcata caacaaacat taactcttca ggatccgatt atgagtcaaa catcaacctt
    39481 gaaaggccag tgcattgctg aattcctcgg taccgggttg ttgatttttt tcggtgtggg
    39541 ttgcgttgca gcactaaaag tcgctggtgc gtcttttggt cagtgggaaa tcagtgtcat
    39601 ttggggactg ggggtggcaa tggccatcta cctgaccgca ggggtttccg gcgcgcatct
    39661 taatcccgct gttaccattg cattgtggct gtttgcctgt ttcgacaagc gcaaagttat
    39721 tccttttatc gtttcacaag ttgccggcgc tttctgcgct gcggctttag tttacgggct
    39781 ttactacaat ttatttttcg acttcgagca gactcatcac attgttcgcg gcagcgttga
    39841 aagtgttgat ctggctggca ctttctctac ttaccctaat cctcatatca attttgtgca
    39901 ggctttcgca gttgagatgg tgattaccgc tattctgatg gggctgatcc tggcgttaac
    39961 ggacgatggc aacggtgtac cacgcggccc tttggctccc ttgctgattg gtctactgat
    40021 tgcggtcatt ggcgcatcta tgggcccatt gacaggtttt gccatgaacc cagcgcgtga
    40081 cttcggtccg aaagtctttg cctagctggc gggctggggc aatgtcgcct ttaccggcgg
    40141 cagagacatt ccttacttcc tggtgccgct tttcagccct atcgttggcg cgattgtagg
    40201 tgcatttgcc taccgcaaac tgattggtcg ccatttgcct tgcgatatct gtgttgtgga
    40261 agaaaaggaa accacaactc cttcagaaca aaaagcttcg ctgtaatatg actacgggac
    40321 aattaaacat gactgaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    40381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    40441 aatctaccca aaaccaggtt gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    40501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    40561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    40621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    40681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    40741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcacgagc gtgcacgtcg
    40801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    40861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    40921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    40981 gcgtcgttct tccgaagtat acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    41041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    41101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acactggcga
    41161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    41221 agtgaactat gcgttggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    41281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    41341 aaacaccaat ggtgtgtatg tggttccggc atttaccggg ctgggtgcgc cgtactggga
    41401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    41461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    41521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    41581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagtgcgcga
    41641 agtcaccgca ttgggtgcgg cctatctcgc aggcctggcg gttggcttct ggcagaacct
    41701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    41761 tgagcgtaat taccgttacg caggctggaa aaaagcggtc aaacgcgcga tggcgtggga
    41821 agaacacgac gagtaatgtc aggcacctga atcgattgcc tgatgctgat cgcctgatgc
    41881 gacgcttacg cgtcttatca ggcctacgag ccaatgccat tttgtgtagg cgggataagg
    41941 cgttcacgcc gcatccggca tcacgcagac tctgaaaccg tgcgggggca accccgcaca
    42001 catcaataat cccacccttc ccctgtgcta cacttcgcgc cattccttac tgcttagagt
    42061 ttgctatgag acgagaactt gccatcgaat tttcccgcgt caccgaatct gcggcgctgg
    42121 ctggctacaa atggttagga cgcggcgata aaaacaccgc ggacggcgcg gcggtaaacg
    42181 ccatgcgtat tatgctcaac caggtcaaca ttgacggcac catcgtcatt ggtgaaggtg
    42241 aaatcgacga agcaccgatg ctctacattg gtgaaaaagt cggtactggt cgcggcgacg
    42301 cggtagatat tgctgttgat ccgattgaag gcacgcgcat gacggcgatg ggccaggcta
    42361 acgcgctggc ggtgctggca gtaggcgata aaggctgctt cctcaatgcg ccggatatgt
    42421 atatggagaa gctgattgtc gggccgggag ccaaaggcac cattgatctg aacctgccgc
    42481 tggcggataa cctgcgcaat gtagcggcgg cgctcggcaa accgttgagc gaactgacgg
    42541 taacgattct ggctaaacca cgccacgatg ccgttatcgc tgaaatgcag caactcggcg
    42601 tacgcgtatt tgctattccg gacggcgacg ttgcggcctc aattctcacc tgtatgccag
    42661 acagcgaagt tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgtg gtttctgcgg
    42721 cggtgatccg cgcattagat ggcgacatga acggtcgtct gctggcgcgt catgacgtca
    42781 aaggcgacaa cgaagagaat cgtcgcattg gcgagcagga gctggcacgc tgcaaagcga
    42841 tgggcatcga agccggtaaa gtattgcgtc tgggcgatat ggcgcgcagc gataacgtca
    42901 tcttctctgc caccggtatt accaaaggcg atctgctgga aggtattagc cgcaaaggca
    42961 atatcgcgac taccgaaacg ctgctgatcc gcggcaagtc acgcaccatt cgccgcattc
    43021 agtccatcca ctatctggat cgcaaagacc cggaaatgca ggtgcacatt ctctgagtga
    43081 tttgatcgat tgagccttcc agtccttcgg gactggaatt tttttgttcg gagaacgaag
    43141 ataaggcaag tcaatcaaaa caggagaaaa acatggctga ttgggtaaca ggcaaagtca
    43201 ctaaagtgca gaactggacc gacgccctgt ttagtctcac cgttcacgcc cccgtgcttc
    43261 cgtttaccgc cgggcaattt accaagcttg gccttgaaat cgacggcgaa cgcgtccagc
    43321 gcgcctactc ctatgttaac tcgcccgata atcccgatct ggagttttac ctggtcaccg
    43381 tccccgatgg caaattaagc ccacgattgg cggcactgaa accgggcgat gaagtgcagg
    43441 tggttagcga agcagctggc ttctttgttc tggatgaagt accggactgc gaaacgctat
    43501 ggatgctggc aaccggtaca gcgattggcc cttatttatc gattctgcaa ctaggcaaag
    43561 atttagatcg cttcaaaaat ctggtcctgg ttcacgccgc acgttatgcc gccgacttaa
    43621 gctatttgcc actgatgcaa gaactggaaa aacgctacga aggcaaactg cgcattcaga
    43681 cggtggtcag tcgggaaacg gcagcggggt cgctcaccgg acggataccg gcattaattg
    43741 aaagtgggga actggaaagc gcgattggcc tgccgatgaa taaagaaacc agccatgtga
    43801 tgctgtgcgg caatccacag atggtgcgcg atacacaaca gttgctgaaa gagacccggc
    43861 agatgacgaa acatttacgt cgccgaccgg gccatatgac agcggagcat tactggtaag
    43921 cggttactta tcgataaacg gcacgatgag caaatccgca ctcatcttat tgatcatccc
    43981 gcgatatgcc ggcatcaaac ggttgataaa tgagtgatga tgaccacaga caaggaggtc
    44041 gcactgctct ttttgcataa tttccagcag tgtttccggc atttctccgc gttcaatacg
    44101 cagttttgtc ttcggccatt gaatattttt cgtcagttta tacagcttgt tatccgactt
    44161 attcttcaac aattgaagaa tatcttctgt tgcagggaag tagatacccg gatacaactc
    44221 gcttaagcca tcatcaatat gaattaacgt caggtgagcg tcattatgtc tggcgagctc
    44281 cagggcttta ttcaccagta aggcatcttc ttcattcccg gaaattgcca cgccaatgtg
    44341 tttataagcc atgtttaact ccttctaaag caactccatc aagctaacga acgcagtgat
    44401 aactcaaaaa taatcatctc ggcctggcat gagaaagtga aggccgcatc aagctcaact
    44461 tgcccctcca gatccttaat cttataggcc acgctggccc cttcatctgc agaggatgca
    44521 gcggcggcaa gctctttcag tttggtcata aaagactctg cttcttcgcg ggtggcaaaa
    44581 aagcgcgaaa acttactggt acagttgtcg ttatcaatca ccgtaccgat atcaatcgca
    44641 catcctttag tactgcattt atctacgaca tctttcatag ggcacctctg tatattcgcc
    44701 tctctgtcga ttacgactta agaataatgc gccgttaagg gaagaacaaa caagtaatga
    44761 tcttatggat taccattttt tttgatattg cttaaggtga agagttcaga aggattttcc
    44821 gggtattgtc atttgcatcg tggaaataac aatcccggaa aaaaatagca gtaagagata
    44881 tattaaaggt taattacttg atttattgtc ggctttatac ttcacatcct gagtatcttt
    44941 accatattta ttttcgcctt gtgtgccaac aaacgcacca agatcgataa gcatcatcac
    45001 cagaatcaac gtcgggacaa aacgccccac cgcccattgc cagacacccg gtaaaatcgc
    45061 tcagttaccc gccagcagca tccacgccac aatcatcaga aatgcccatg cgccggaacg
    45121 cccgcgatca tgcaagcgct taacagttac tgccgctgtt ggccagagca agcacacaag
    45181 gcaaaacgcc gcggtctgaa tatcgagtag attcttaccc gccagtgaga aaagcaccag
    45241 catgcctgcg aaccacaggc ctatccaaat ccagaaatca cggcgtccaa tacgcccttt
    45301 aaatgagaat aaccattgct gtatggtcat gtaagttcct tgatggttgt cttttccagg
    45361 attctaccct tttgacaagg gcgacagata tcgttttaat cggagccagt cataacaaaa
    45421 ggtactgtca atgaagccag ggtgtacgct gttttttctc ttatgttctg cattaaccgt
    45481 tacaacaacg gcgcatgcac aaacaccaga tacggcaacg accgcgcctt atctgctggc
    45541 tggagcccct actttcgatc tctccatcag ccagtttcga gaagacttta acagccagaa
    45601 tcccagcctg ccactgaacg aatttcgtgc catcgacagc agtcccgaca aagccaatct
    45661 cactcgtgct gcaagtaaaa ttaacgagaa cttgtatgct tctacagcgc tggagcgcgg
    45721 taccttaaaa atcaaaagca ttcaaatgac ctggctaccc atccaggggc cagagcaaaa
    45781 agcagcgaaa gcgaaagctc aggaatacat ggcagcagtg atccgcacac tcaccccatt
    45841 aatgaccaaa acacaaagcc agaaaaaact gcagtcgcta ctaacggcgg ggaaaaacaa
    45901 acgttattac accgagacag aaggtgcact gcgttatgtt gtcgcggaca acggcgaaaa
    45961 ggggctgacc ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc ttgaaggttt
    46021 gaataaatga caaaaagcaa agcctttgtg ccgatgaatc tctatactgt ttcacagacc
    46081 tgctgccctg cggggcggcc atcttccttt attcgcttat aagcgtggag aattaaaatg
    46141 cgacatcctt tagtgatggg taactggaaa ctgaacggca gccgccacat ggttcacgag
    46201 ctggtttcta acctgcgtaa agagctggca ggtgttgctg gctgtgcggt tgcaatcgca
    46261 ccaccggaaa tgtacatcga tatggcgaag cgcgaagctg aaggcagcca catcatgctg
    46321 ggtgcgcaaa acgtggacct gaacctgtcc ggcgcattca ccggtgaaac ttctgccgct
    46381 atgctgaaag acatcggcgc tcagtacatc atcatcggcc actctgaacg tcgtacttac
    46441 cacaaagagt ctgacgaact gattgcgaaa aaattcgcgg tgctgaaaga gcagggtctg
    46501 actccggttc tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggaaa aactgaagaa
    46561 gtttgcgcac gtcagatcga cgcggtactg aaaactcagg gtgctgcggc attcgaaggt
    46621 gcggttatcg cttacgaacc tgtatgggca atcggtactg gcaaatctgc aactccggct
    46681 caggcacagg ctgttcacaa attcatccgt gaccacatcg ctaaagttga cgctaacatc
    46741 gctgaacaag tgatcattca gtacggcggc tctgtaaacg cgtctaacgc tgcagaactg
    46801 tttgctcagc cagacatcga cggcgcgctg gttggcggtg cttctctgaa agctgacgct
    46861 ttcgcagtga tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg acaggtgccg
    46921 gatttcatat ccggcactta ctttccttaa ctcctcgcct taacgcaaaa tctcacactc
    46981 atgatcctgg atttcctcgg ctgaagcacg gttcagcgtc agtagatttc gttgtgtcgc
    47041 cagcaacaca aacgaattat cactctgccg taccatcgcc agcccgtagc ttcccatatg
    47101 ttcccgcgcc tcaggcactt cttctgcaag catcataaat gggctgcgtt gtaccagttc
    47161 gctttccgtt acccgacgcg ccaggtattc atgcccgcgc aaaccacctg gcagtggcaa
    47221 ccagcggctg ctgatgttcg ccagattttt atccagttgt tcgcgcacat caggacgaat
    47281 acaagagata tgaatatgaa aatggttttg cgtatgcccg gtgcgggagt tgatcgccaa
    47341 agaaaccgcg cgatcgggaa ccggctggcc gtattttttg ctcataaaat cacgcgcctg
    47401 ccaggccaac caaaagaagt tcggcgttga aggatcggtc aacaaaggac tttcagtacc
    47461 gttaatacga tacgttggca tcaacagata ttgcagtgga ccattaagat cttttaaaac
    47521 cacgtatccg gcattgggtt tgacttccgc acatggcgaa ggattttgat tttgctgctg
    47581 attgggcaaa cattcctcaa ggacaatctt acgtaatgta tccgactctt caccggttaa
    47641 tttccagtaa ccaataccgg cagccacaac ggcgataact atcatcacca aaaaaagaag
    47701 acccgctttt ttcatctttt tttccctgta cctcaaagag atgcaagggt aacgcaaaat
    47761 cgtgacaaat aaaaaacccg gtcgcgagaa accaccgggt ttgataatta tcctagggaa
    47821 atcagcgttt gctgatctga tcgaacgtac cgccgttagc aaaatgctct ttttgcgctt
    47881 tcgtccagcc gccgaactct tcatcaatgg tgaataactt cagctttgga aacgcatttt
    47941 cgtacttttt cgctacttca gcgtcgcgcg gacggtagta gtttttcgcg gcaatttcct
    48001 gaccttctgg cgagtagaga tatttcaggt aggcttccgc cacctctttg gtgccttttt
    48061 tctcgaccac tttatcgacc accgacaccg ttggctctgc gaggatagac tcactcggcg
    48121 tgacgatttc gaatttatct ttccccagtt cattcgctgc cagcagagct tcgttttccc
    48181 aggcgatcag tacatcgcca atcccgcgct cgacaaaggt gttagtggag ccgcgcgcgc
    48241 cagaatccag aacttcgacg tttttataca gtgcccgaac aaaatcctgt gcttttgcct
    48301 gatcgttgtt gttgtgatgc agcgcgtagc cccaggcagc caggtagttc cagcgtgcgc
    48361 caccagagct tttcggatta ggcgtgatca ccgaaacacc cggtttaatc agatcgttcc
    48421 agtcatggat ctgcttcgga ttgcccttac gcaccaggaa aacaatggtg gaagtgtacg
    48481 gtgcggagtt atccggcaga cgtttgatcc actctttatc aatccgcccg cgttccgcaa
    48541 tcgcgtccac gtcataggcc agagccagcg tgacaacatc agcttcaata ccgttgatta
    48601 ccgacgtcgc ttgtttacct gaaccaccgt gtgactgacg aatcaccacg ttatcaccag
    48661 tttgctgttt ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat tcgcgcgttg
    48721 gatcatatga aacgttaaga agctgaatat cctttgccat aacgctggtt gccgccagca
    48781 aaaatgttaa ccctacgccc cacttgttca tcgcccgact ctcttatgtt gtgttgtgat
    48841 gagcaaagcg tgccagaagg ttaaccaaac attaaagaat aaaaaaagat tggctataac
    48901 ttgcgggtat atgttgaggg attaaaaagg cggaagaaat ccgcctcata ttgctgacaa
    48961 agtgcgcttt gtccatgccg gatgcggcgc gaacgcctta tccggcctac aaaagtttgc
    49021 aaattcaata aattgcagaa ttcatgtagg cctgataagc gaagcgcatc aggcattttt
    49081 gcttctgtca tcggtttcag gctaaaggaa tctgcctttt tccgaaatca ttaatacagt
    49141 tttttcgcgc agtccagcca gtcacctttg aacggacgct tcatgttttc gatagcgtcg
    49201 atgatgtcgt ggtgaaccag ctgttcgttc tggataccta cacaacgacc gccgtaacct
    49261 gccagcagca gatcgatagc gtaagcgccc atacgggaag ccagaatacg gtcgtaaggc
    49321 accggagaac caccgcgctg gatgtggccc agcacagttg cgcgggtttc acgaccggtt
    49381 tctttctcga tgaaatgcgc cagttcgtca acatcacaca tatgttcggt aatcgccacg
    49441 atcgcgtgtt ttttaccttt cgcgataccc gctttgattt cgtttaccag gtcttcacgg
    49501 ctgaattcaa cttccggaac tacaacgaat tcacagccac cggcaatggc cgcagccagg
    49561 gtcagatcgc cacaataacg gcccatcact tccaccacgg aaatacgctg gtgagaagaa
    49621 gaggtgtcac gcagacggtc gatcgcttct acaacggtgc tcagcgcagt gaagaaaccg
    49681 atagtgtagt cagtgccttt gatgtcgttg tcgatagtgc ccggcagacc gatgcacggg
    49741 aagcccattt cggtcagacg cattgcaccc atgtaggaac cgtcaccgcc gataaccacc
    49801 agcgcgtcga taccacgttt tttcaggttt tcgatagcca cggcgcggat gttctcgtcg
    49861 cggaattccg ggaaacgcgc agaaccgagg aacgtaccgc cacggttgat catgtcagac
    49921 acgctgtaac ggtctagctg taccatacgg tcttcataca gacccagata gccgtcataa
    49981 atacccatta cttccagacc ttctgtcagc gcagaacgaa caaccccgcg aattgcggcg
    50041 ttcatgcctg gcgcatcacc gccgcttgtc aacacaccta ttttcttaat catgactacc
    50101 tctgaacttt ggaatgcaaa atgaaatctg ttgccggaag tcttcttgca catcgaagtg
    50161 atccaacgaa tgtgcaaata gtataacaat cacttcctgc tgaattgatt caggtcaggc
    50221 caaatggcgg tattttatac acaaaatgcg ggtctggctc tcttttatac tgattatgaa
    50281 agcatagacc gtttaccctc cctgggtacg acggaacagg ggtcctgatg gataatcaca
    50341 tccgatcccg gaaaacgccg taaaatagcc tgctctacct gatccgccac catatgtgcc
    50401 tgaaccaaag gcagagagtc ttccatttcc aaatgaatct gaataaagcg ggtcggccct
    50461 gactgccgcg tgcgaagatc gtgagcgccg ctaacacccg gccaggaagt cacgatatca
    50521 ataatttctt gccgttcttc atcaggcaat gcgcgatcca gtaatgactg taccgcctca
    50581 tatcccatgc gtaacgcgct atataaaata tagatgccga ttcccaatgc aaacagagca
    50641 tcggcgcgat gccagccgta ccaagacaac cccagcgcca gcagaattgc gccgttcatc
    50701 ataacatcag actggtaatg tagcatatca gcccgcaccg cctggctttg cgtccggcgc
    50761 accacccacc gctgaaacga gacaaggata atcgtacaaa ttagcgccac aattgtcacg
    50821 ataaccccga cgcctggatc tgtcatcggt gttggagata ccagatgttg aatacccgtc
    50881 aaaaacagga atagtgccga accggagata aacatacttt gcgccagcgc cgcgagggac
    50941 tcagctttac cgtgaccaaa cgagtgatta tcgtcggcag gttgcaggga atatcgcacc
    51001 accagtaaat tcgtcaacga cgcgccgata tccaccagcg aatccaccag cgcggcgaga
    51061 atactcaccg acccggtata ccaccatgca aaaattttaa tcagcaatag caacgaagcc
    51121 atcgccgtcg cagcaatcgc cgcccgactg accagccgtc cataagattg attcataaat
    51181 actcccgcta tcaactgacg ctagtataac ggaagcaaat catctgcaat gcattaagca
    51241 gcaggcaaat tgaggataaa aaaaaaccca catcatgtgg gggaagacag ggatggtgtc
    51301 tatggcaagg aaaacagggt ttactactgg gaacgtgagt tgctactact caatagcttc
    51361 aacgatgaac ttttttgcca ttgcgtcacg tcacgcaact gctccattcg ttgttgatgt
    51421 ttctcgttta aaaccgcttg ctgctccggc gttaacaggc gatacatttg gttgcggact
    51481 tttgccatct caacctgacg agcaatttgc tcattcgcca ttttttctgc ctgtgcgcgc
    51541 acagcgtttt catcaaaatt ttctgcggtg acaaggcgat gcattgtctc cagttcgcta
    51601 acattaacag gaggctgttc gtgccgggcc tgttgcataa gatctcgcat ctgctgacgc
    51661 tgatgttcgg ttaaacttat gccgtcgaac atatggctct gcgtactgcg ctgcgtaagt
    51721 tcttcacccg gatgccagtt atcgcctgaa ccgacttcag cagcgtggct taatgaactg
    51781 actgccagcg ttgaggccat gacggcagcg gtaactatgc gcatcatttg ctcccaaaat
    51841 ctttctgtcg cgattcaacg atagagagtt tacgattcag gctgcaaaca tgcgtcaggg
    51901 ggtgtaaaac aacgtaaagt catggattag cgacgtctga tgacgtaatt tctgcctcgg
    51961 aggtatttaa acaatgaata aaatcctgtt agttgatgat gaccgagagc tgacttccct
    52021 attaaaggag ctgctcgaga tggaaggctt caacgtgatt gttgcccacg atggggaaca
    52081 ggcgcttgat cttctggacg acagcattga tttacttttg cttgacgtaa tgatgccgaa
    52141 gaaaaatggt atcgacacat taaaagcact tcgccagaca caccagacgc ctgtcattat
    52201 gttgacggcg cgcggcagtg aacttgatcg cgttctcggc cttgagctgg gcgcagatga
    52261 ctatctcccg aaaccgttta atgatcgtga gctggtggca cgtattcgcg caatcctgcg
    52321 ccgttcgcac tggagcgagc aacagcaaaa caacgacaac ggttcaccga cactggaagt
    52381 tgatgcctta gtgctgaatc caggccgtca ggaagccagc ttcgacgggc aaacgctgga
    52441 gttaaccggt actgagttta ccctgctcta tttgctggca cagcatctgg gtcaggtggt
    52501 ttcccgtgaa catttaagcc aggaagtgtt gggcaaacgc ctgacgcctt tcgaccgcgc
    52561 tattgatatg cacatttcca acctgcgtcg taaactgccg gatcgtaaag atggtcaccc
    52621 gtggtttaaa accttgcgtg gtcgcggcta tctgatggtt tctgcttcat gataggcagc
    52681 ttaaccgcgc gcatcttcgc catcttctgg ctgacgctgg tgctggtgtt gatgttggtt
    52741 ttgatgttac ccaagctcga ttcacgccag atgaccgagc ttctggatag cgaacagcgt
    52801 caggggctga tgattgagca gcatgtcgaa gcggagctgg cgaacgatcc gcccaacgat
    52861 ttaatgtggt ggcggcgtct gttccgggcg attgataagt gggcaccgcc aggacagcgt
    52921 ttgttattgg tgaccaccga aggccgcgtg atcggcgctg aacgcagcga aatgcagatc
    52981 attcgtaact ttattggtca ggccgataac gccgatcatc cgcagaagaa aaagtatggc
    53041 cgcgtggaac tggtcggtcc gttctccgtg cgtgatggcg aagataatta ccaactttat
    53101 ctgattcgtc cggccagtag ttctcaatcc gatttcatta acttattgtt tgaccgcccg
    53161 ctattactgc tgattgtcac catgttggtc agtacgccgc tgctgttgtg gttggcctgg
    53221 agtctggcaa aaccggcgcg taagctgaaa aacgctgccg atgaagttgc ccagggaaac
    53281 ttacgccagc acccggaact ggaagcgggg ccacaggaat tccttgccgc aggtgccagt
    53341 tttaaccaga tggtcaccgc gctggagcgc atgatgacct ctcagcagcg tctgctttct
    53401 gatatctctc acgagctgcg caccccgctg acgcgtctgc aactgggtac ggcgttactg
    53461 cgccgtcgta gcggtgaaag caaggaactc gagcgtattg aaaccgaagc gcaacgtctg
    53521 gacagcatga tcaacgatct gttggtgatg tcacgtaatc agcaaaaaaa cgcgctggtt
    53581 agcgaaacca tcaaagccaa ccagttgtgg agtgaagtgc tggataacgc ggcgttcgaa
    53641 gccgagcaaa tgggcaagtc gttgacagtt aacttcccgc ctgggccgtg gccgctgtac
    53701 ggcaatccga acgccctgga aagtgcgctg gaaaacattg ttcgtaatgc tctgcgttat
    53761 tcccatacga agattgaagt gggctttgcg gtagataaag acggtatcac cattacggtg
    53821 gacgacgatg gtcctggcgt tagcccggaa gatcgcgaac agattttccg tccgttctat
    53881 cggaccgatg aagcacgcga tcgtgaatct ggcggtacag gattggggct ggcgattgtt
    53941 gaaaccgcca ttcagcagca tcgtggctgg gtgaaggcag aagacagccc gctgggcggt
    54001 ttacggctgg tgatttggtt gccgctgtat aagcggagtt aaactccgca tttgtaggca
    54061 ggataaggcg tttacgccgc atccggcatt tgagcaggat gcctgatgcg acgctgatag
    54121 cgtcttatca ggcctacact ccgggggtta ttttccccac aaccggcggg aaaaatcttc
    54181 gatcttgccg ctcagtcggc gcttttgcat cgtccgcgtc cagcttttcg ataacccggc
    54241 ggcggagagt aaacggtgat actgatcgtc atcaaacggc atatgccatg cgatggcggc
    54301 agcctcctgc acggtcacat cgctgacacg ggaaaccaac tccagcggcg cgtccgcaga
    54361 cactttccct ggcgctatca cgctatacaa ccaccccacc ttacc
//
LOCUS       DAJTUP010000015        53200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTUP010000015 DAJTUP010000000
VERSION     DAJTUP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.015"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(11..322)
                     /locus_tag="OO468_000926"
     CDS             complement(11..322)
                     /locus_tag="OO468_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR6258138.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO468_000927"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO468_000927"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO468_000928"
     CDS             701..1054
                     /locus_tag="OO468_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR6258139.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO468_000929"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO468_000929"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR6258140.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO468_000930"
     CDS             complement(2810..3280)
                     /locus_tag="OO468_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR6258141.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO468_000931"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO468_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO468_000932"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO468_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6258142.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO468_000933"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO468_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR6258143.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5282..8431)
                     /locus_tag="OO468_000934"
     CDS             complement(5282..8431)
                     /locus_tag="OO468_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR6258144.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="OO468_000935"
     CDS             complement(8454..9647)
                     /gene="acrA"
                     /locus_tag="OO468_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR6258145.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9789..10436
                     /gene="acrR"
                     /locus_tag="OO468_000936"
     CDS             9789..10436
                     /gene="acrR"
                     /locus_tag="OO468_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR6258146.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10564..13926
                     /gene="mscK"
                     /locus_tag="OO468_000937"
     CDS             10564..13926
                     /gene="mscK"
                     /locus_tag="OO468_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR6258147.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="OO468_000938"
     CDS             complement(14138..14299)
                     /gene="rsmS"
                     /locus_tag="OO468_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR6258148.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14313..14840)
                     /gene="priC"
                     /locus_tag="OO468_000939"
     CDS             complement(14313..14840)
                     /gene="priC"
                     /locus_tag="OO468_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR6258149.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14910..15287
                     /locus_tag="OO468_000940"
     CDS             14910..15287
                     /locus_tag="OO468_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR6258150.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15440..15991
                     /gene="apt"
                     /locus_tag="OO468_000941"
     CDS             15440..15991
                     /gene="apt"
                     /locus_tag="OO468_000941"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR6258151.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16120..18045
                     /gene="dnaX"
                     /locus_tag="OO468_000942"
     CDS             16120..18045
                     /gene="dnaX"
                     /locus_tag="OO468_000942"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR6258152.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17378..17442
                     /gene="dnaX"
                     /locus_tag="OO468_000942"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18098..18427
                     /locus_tag="OO468_000943"
     CDS             18098..18427
                     /locus_tag="OO468_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR6258153.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18427..19032
                     /gene="recR"
                     /locus_tag="OO468_000944"
     CDS             18427..19032
                     /gene="recR"
                     /locus_tag="OO468_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR6258154.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19142..21016
                     /gene="htpG"
                     /locus_tag="OO468_000945"
     CDS             19142..21016
                     /gene="htpG"
                     /locus_tag="OO468_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR6258155.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21197..21841
                     /gene="adk"
                     /locus_tag="OO468_000946"
     CDS             21197..21841
                     /gene="adk"
                     /locus_tag="OO468_000946"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR6258156.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21973..22935
                     /gene="hemH"
                     /locus_tag="OO468_000947"
     CDS             21973..22935
                     /gene="hemH"
                     /locus_tag="OO468_000947"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR6258157.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22932..23891)
                     /gene="aes"
                     /locus_tag="OO468_000948"
                     /pseudo
     CDS             complement(22932..23891)
                     /gene="aes"
                     /locus_tag="OO468_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24043..25347
                     /gene="gsk"
                     /locus_tag="OO468_000949"
     CDS             24043..25347
                     /gene="gsk"
                     /locus_tag="OO468_000949"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR6258158.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25480..27156)
                     /locus_tag="OO468_000950"
     CDS             complement(25480..27156)
                     /locus_tag="OO468_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR6258159.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="OO468_000951"
     CDS             complement(27394..28614)
                     /gene="fsr"
                     /locus_tag="OO468_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR6258160.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28832..30484
                     /gene="ushA"
                     /locus_tag="OO468_000952"
     CDS             28832..30484
                     /gene="ushA"
                     /locus_tag="OO468_000952"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6258161.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="OO468_000953"
     CDS             complement(30521..31000)
                     /gene="ybaK"
                     /locus_tag="OO468_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR6258162.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31204..31998)
                     /locus_tag="OO468_000954"
     CDS             complement(31204..31998)
                     /locus_tag="OO468_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR6258163.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32136..32477
                     /locus_tag="OO468_000955"
     CDS             32136..32477
                     /locus_tag="OO468_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6258164.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32692..35196)
                     /gene="copA"
                     /locus_tag="OO468_000956"
     CDS             complement(32692..35196)
                     /gene="copA"
                     /locus_tag="OO468_000956"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR6258165.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35458..36390
                     /gene="glsA"
                     /locus_tag="OO468_000957"
     CDS             35458..36390
                     /gene="glsA"
                     /locus_tag="OO468_000957"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR6258166.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36393..37685
                     /locus_tag="OO468_000958"
     CDS             36393..37685
                     /locus_tag="OO468_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6258167.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37810..38217
                     /gene="cueR"
                     /locus_tag="OO468_000959"
     CDS             37810..38217
                     /gene="cueR"
                     /locus_tag="OO468_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR6258168.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38218..38676)
                     /locus_tag="OO468_000960"
     CDS             complement(38218..38676)
                     /locus_tag="OO468_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR6258169.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38673..39590)
                     /locus_tag="OO468_000961"
     CDS             complement(38673..39590)
                     /locus_tag="OO468_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6258170.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39736..40413
                     /gene="fetA"
                     /locus_tag="OO468_000962"
     CDS             39736..40413
                     /gene="fetA"
                     /locus_tag="OO468_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR6258171.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40400..41179
                     /gene="fetB"
                     /locus_tag="OO468_000963"
     CDS             40400..41179
                     /gene="fetB"
                     /locus_tag="OO468_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR6258172.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="OO468_000964"
     CDS             complement(41242..42096)
                     /gene="cnoX"
                     /locus_tag="OO468_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR6258173.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="OO468_000965"
     CDS             complement(42157..42966)
                     /gene="ybbO"
                     /locus_tag="OO468_000965"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR6258174.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="OO468_000966"
                     /pseudo
     CDS             complement(42956..43578)
                     /gene="tesA"
                     /locus_tag="OO468_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43549..44235
                     /locus_tag="OO468_000967"
     CDS             43549..44235
                     /locus_tag="OO468_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6258175.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQLFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44232..46647
                     /locus_tag="OO468_000968"
                     /pseudo
     CDS             44232..46647
                     /locus_tag="OO468_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO468_000969"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="OO468_000969"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR6258176.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO468_000970"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="OO468_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR6258177.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="OO468_000971"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="OO468_000971"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR6258178.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="OO468_000972"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="OO468_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR6258179.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="OO468_000973"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="OO468_000973"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR6258180.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53200
                     /locus_tag="OO468_000974"
     CDS             52938..>53200
                     /locus_tag="OO468_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6258181.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVA"
BASE COUNT        12826 a        13687 c        14013 g        12674 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaggcc gcttacgcgg ccttagtgat tacatgttgt
     5281 atcaatgatg atcgacagta tggctgtgct cgatatcttc attcttgcgg ctaaagcggc
     5341 ggcgaaccac cacaaagaat accggaacga agaagattgc cagtaccgtt gcggtcacca
     5401 tcccgcccat tacaccggta cctactgcgt tctgcgcgcc ggaaccagca ccagtactga
     5461 taaccagcgg cataacgccg aggataaacg ccagcgaggt catcaggatc ggacgtaaac
     5521 gcatccgcac cgcatcaagc gtcgcttcaa tcagaccttt accttcttta tccatcaagt
     5581 ctttggcgaa ttcgacgata agtatcgcgt tcttcgccga caacccaatg gttgtgagca
     5641 gtcctacttg gaagtaaacg tcattggtca ggccacggaa ggtggcagcc agcaacgcac
     5701 cgataacccc cagcggaacg accagcataa cggagaacgg agtcgaccag ctctcgtaca
     5761 gcgccgccag acacaggaat acgacaatca acgaaatcgc gtacagtgaa ggtgcctggt
     5821 tgccggagag acgttcctga taggacatcc ccgtccagtc atagccaaca ccggtaggca
     5881 gtttgctcgc cagttgttcc atcagctcca ttgcttcacc ggtactttta cccggtgccg
     5941 cctggcctaa gatttccatg gatggcaggc cgttgtaacg ttccagacgc ggcgaaccgt
     6001 actcccaacg agaagaggag aacgccgaga atggcaccat ctgaccatca gcagcacgaa
     6061 cataccagtc gccgatatca tccggcagca tacggtattt cgcttctgac atgacgtaaa
     6121 ctttcttcac acgaccgcgg tcgataaagt cgttcacata gctaccgccc catgcagcgc
     6181 ccagagtggt gttaatgtcg ttgatagaaa cacccagcgc ctgcgctttt tcctggtcga
     6241 tatcaatctt aaactgcggg gtatcttcca gaccgtttgg acgtacgctg gtcagcatat
     6301 cagggtgctt cgctgcttct gcaagcagct ggttacgcgc ctgagtcagt ttttcgtgac
     6361 caaggccagc ctggtcaatc agctcaaagt caaagccggt tgcagtaccc agttccacga
     6421 ttgcgggcag gttaaaggcg aaaaccatcg catctttgat ttgcgagaaa gcgcgtgttg
     6481 cacgcatggt aatcgcttca actttgtttt cttcgcccgg acgatcggcc cagtccttca
     6541 aggaaacgaa cgcaataccg gtattctgac cacgtcccgc aaagccgaag ccgttaacgg
     6601 cgaacaccga ctcaacgttg ttcttttctt tggtcagata gtaatgcgtt acctcattga
     6661 gcactttctg cgtacgttcc tgcgttgcac ctgctggcag ctgaaccatg gtcataaata
     6721 cgccctggtc ctcatctggc aagaaggagc ttggcagacg cacgaacaga taggccatgc
     6781 cgaccacgat gatcagatac agcactaggt aacgccccgt actgcgcaga ataccgccta
     6841 cgctgtcggt gtagtggtgc gtgctcttct cgaacatgcg gttcaaccag ccgaagaagc
     6901 cttttttacc ttccccgtga tcgcctttgg caatcggttt cagcatggtg gcacaaagag
     6961 ccggagtcag gatcaacgct accagtaccg acagcgccat tgctgaaaca atggtaatag
     7021 agaactgacg atagatagca ccagtagaac cgccaaagaa ggccatcggt acgaataccg
     7081 ccgacagtac catcgcgata ccgaccagag cgccctgaat ctgccccatc gacttacggg
     7141 tagcttcttt tggcggcaaa ccttcttccg ccataacacg ctccacgttt tctaccacaa
     7201 cgatagcgtc atccaccaac aagccgatgg cgagcaccat cccgaacatt gttagcgtgt
     7261 ttatcgagaa gccaaaggcg gcaaggacgg caaaggtccc gagcaatacc accggtacgg
     7321 caatggtcgg aatcaacgtc gcgcggaagt tctgcaggaa cagatacata accaggaaca
     7381 cgaggatgat cgcttcgacc agcgttttaa ccacttcgtg aatagagatt ttcacgaacg
     7441 gcgtggtgtc gtacgggtaa acaattttca gacccgacgg gaagaacggt tccatcttcg
     7501 ccagttcagc acggattgcc gcagcggtat ccagcgcgtt tgcaccggtc gccagcttga
     7561 tccccagacc ggaagcaggt tggccgttaa actctgcgat gatgtcgtag ttctcaccac
     7621 ccagctcaat cttcgccaca tcacgcagca gtacgcggga accatcctga ttcactttca
     7681 gcaggatttt gccgaactct tcagtagagg tcagacgcgt ctgagcaata atagaggcgt
     7741 taagctgttg gcctttcacc ggcggcgtac caccgagctg acccgccgca acctgggcgt
     7801 tctgcgcttt gatggcggta atgacatcaa ccggcgttag ctggaatttg ttcagctcat
     7861 tcgggttcat ccagatacgc atcgcgtact gtgaaccgaa caactgaaca tcacccacgc
     7921 ccgacgtacg gctgatggca tctttcatat tcgccgctac gtagtcagag atatcctcct
     7981 gcgtcatggt gccatcggtg ttgataacgc cgacaaccat caggaagctg ctggatgatt
     8041 tctcaacgct caccccttgc tgctgaactt cttgcggcag caacggcatc gccagctgca
     8101 gtttgttctg cacctgaacc tgcgcgatat ccgcatcagt accagactca aaggtcaggg
     8161 tgatctgcac ggtacccgtg gagtcactgt tagaggacat gtacatcagg ttatcgatac
     8221 cgttcatatt ctgttcgata acctgtgtca ccgtgtcctg cactgttttc gcatcagcgc
     8281 cggggtagga ggcggagatc gttactgccg gcggtgcaat cgtaggatat tgcgccaccg
     8341 gcagtttgag gatcgccagc ccccctgcca acatgatgat aatggcgatc acccacgcaa
     8401 aaatcgggcg atcgataaag aaattaggca tgtcttaacg gctcctgttt aagttaagac
     8461 ttggactgtt caggctgagc accgcttgcg gcttgctggt tattatcagc ggtaacttct
     8521 tgtgctttta cctggacacc aggacgcact ttctgcagcc cacttattac tacgcgatcg
     8581 cctgctttca gaccttctgt caccagccac ttatcgccaa tagcctggct tgcaacgatc
     8641 ggacgggttt ccactttgtc atccgcgcca actaccagta cggtggcatc accacgcggc
     8701 gtacgggtta cgccctgttg cgggactaaa atagcgtttg gattaagccc ttcttccaga
     8761 cgtgcgcgca cgaacatacc cggcagcaga gtgtgatccg ggttcgggaa gatagcgcgt
     8821 agggtgatag acccagtggt ctgatcaacg gtaacgtcag agaattccag cgtaccgtcc
     8881 tgcgggaact taatgccgtc actggtgatc agcgacactt tggctttgcc gttctcttgt
     8941 ttcagcgtgc cattcgccag ttcctgtttc aggcgaagga agtcgttgct ggactgggtc
     9001 acatcaacgt agatcggatc aagttgctgc acggttgcca gcacagtcgc ctgaccgttc
     9061 tgtaccaatg cgccttccgt cacgttcgac ttaccaatgc gaccgctaat cggagaggtg
     9121 actttggtgt aagccagatt gatccgcgca gtttcaacgg cagctttcgc cgcagttacc
     9181 gcagcattcg cctgttgcgc atcagccaga gcctgatcgt acccttgctt actgatgtac
     9241 tgagtaccga gcagtttctg ataacgattc accgtcaatt gcgcgatatt ggctgcagcc
     9301 tgggctttcg ccagatcacc tttcgcactg tcgtatgtcg cctgataggt cgcaggatca
     9361 atctgataga gagagacacc agcttcgatg tcgctacctt ctttgaaatt acgcttcagg
     9421 ataatcccgc taacttgagg acgaacttct gcgatccggt aggcactggt gcgacctgga
     9481 agctcggttg tgatctgcag aggttcagtt ttgactgtta ctacgccaac ggcgggcatc
     9541 tgctggccac cttgttgggc ctgtttgtcg tcacatcctg ttagggctaa gctgcctgag
     9601 agcatcagaa cgaccgccag aggcgtaaac cctctgtttt tgttcatatg taaacctcga
     9661 gtgtccgatt tcaaattggt caatggtcaa aagttaataa acccattgct gcgtttatat
     9721 tatcgtcgtg ctatggtaca tacattcaca aatgtatgta aatctaacgc ctgtaaattc
     9781 acgaacatat ggcacgaaaa accaaacaag aagcgcaaga aacgcgccaa cacatcctcg
     9841 atgtggctct acgtcttttc tcacagcagg gggtatcatc cacctcgctg ggcgagattg
     9901 caaaagcagc tggcgttacg cgcggtgcaa tctactggca ttttaaagac aagtcggatt
     9961 tgttcagtga gatctgggaa ctgtcagaat ccaatattgg tgaactagag cttgagtatc
    10021 aggcaaaatt ccctggcgat ccactctcag tattaagaga gatattaatt catgttcttg
    10081 aatccacggt gacagaagaa cgacgtcgat tattgatgga gattatattc cacaaatgcg
    10141 aatttgtcgg agaaatggct gttgtgcaac aggcacaacg taatctctgt ctggaaagtt
    10201 atgaccgtat agaacaaacg ttaaaacatt gtattgaagc gaaaatgttg cctgcggatt
    10261 taatgacgcg tcgggcagca attattatgc gcggctatat ttccggcctg atggaaaact
    10321 ggctctttgc cccgcaatct tttgatctta aaaaagaagc ccgcgattac gtcgccatct
    10381 tactggagat gtatctcctg tgccccacgc ttcgtaatcc tgccactaac gaataaccct
    10441 gaatctgact ccaggatttt tcctggacat tttcgtcgtt gctattctgg ttcactgcgt
    10501 cgtgatattc ttgcggtttg actttttcag gtcgttcttc aggttcagaa accttcattc
    10561 atcatgacta tgttccagta ttacaaacga tcacggcatt ttgttttttc agcatttatt
    10621 gcttttgttt ttgtcttgtt atgccagaac acggcgtttg cgcgggcgtc atcgaatggt
    10681 gatctgccga caaaagcgga cctgcaggcg caacttgact cactaaataa acaaaaagat
    10741 ctttctgctc aggacaaact ggtgcagcag gatctgacag atacattagc caccctcgat
    10801 aaaatcgatc gcataaaaga agagacagtt cagctacggc aaaaagtcgc tgaagcgccg
    10861 gaaaaaatgc gccaggcgac cgcggcgtta acagcactta gcgatgtcga taacgacgaa
    10921 gaaacgcgca aaattctgag tacgctttcg ttgcgccagc tggaaactcg cgttgcccag
    10981 gcgctggacg atttgcaaaa cgcacaaaac gatctggcgt cttataacag ccagctggtt
    11041 tcgttacaga cgcagcccga acgcgtgcaa aatgcgatgt ataacgcttc gcagcagctg
    11101 caacaaattc gcagtcgtct ggatgggact gatgtcggcg agactgcctt acgtcccagc
    11161 cagaaagtgt taatgcaggc tcagcaggcg ttgctgaatg cggagattga ccagcagcgt
    11221 aaaagcctgg aagggaacac cgtcttgcag gataccttgc aaaagcaacg tgattacgtg
    11281 acggcgaaca gcgcccgtct ggagcaccag ttgcagctgt tgcaagaagc ggtaaacagc
    11341 aagcgcctga ctttaactga aaaaacggcg caggaagccg tctccccgga tgaagccgcg
    11401 cgtattcagg ctaatccgct ggtgaaacag gaactggaaa ttaaccagca gttaagtcag
    11461 cgtctgatta ccgcgactga aaacggtaat cagttgatgc agcaaaacat taaagtcaaa
    11521 aactggctgg agcgggcgct gcaatcggaa cgcaatatta aagagcagat tgccgtcctg
    11581 aagggcagcc tgctgttgtc tcgtatcctt taccagcaac aacaaacgct gccctcggcg
    11641 gatgaactgg aaaacatgac caaccgcatc gcggatttgc gtctcgaaca gtttgaagtt
    11701 aaccagcagc gtgatgcact cttccagagc gatgcgttcg tcaacaaact ggaagaaggt
    11761 cacaccaacg aagtcaacag cgaagttcac gatgcgttat tgcaagtggt tgatatgcgt
    11821 cgcgaattgc tggatcaact caacaaacag ttgggtaacc agctgatgat ggccattaac
    11881 ctgcaaatca accagcagca gttaatgagc gtatcgaaaa acctgaaatc catcctgact
    11941 cagcaaatct tttgggtgaa cagtaaccgt ccaatggact gggactggat caaagcgttc
    12001 ccgcaaagcc tgaaagatga atttaagtcg atgaaaatca cggtgaactg ggaaaaagcc
    12061 tggcctgccg tttttatcgc tttcctcgcc ggtttgccgc tgctgttgat tgccgggctg
    12121 atccactggc gtctgggctg gctgaaagcg tatcaacaga aactggcttc cgctgtgggt
    12181 tccctgcgta acgatagcca gctcaacaca ccaaaagcga tccttatcga cctgatccgc
    12241 gcgctgccgg tgtgcctgat tattctcgcg gttggcctga ttctgttgac catgcagctc
    12301 aacatcagcg aactgctatg gtcgttcagt aaaaaactgg cgattttctg gctggtgttt
    12361 ggcctgtgct ggaaggtact ggagaaaaat ggcgttgccg tgcgtcactt cggcatgccg
    12421 gagcagcaga ccagccactg gcgtcggcaa attgtccgca tcagtctcgc gttgctgcct
    12481 atccatttct ggtctgtggt ggcagaactt tccccgctgc atctgatgga tgatgtgctg
    12541 gggcaggcga tgattttctt caacctgctg ctgattgcct tcctggtatg gccaatgtgc
    12601 cgcgaaagct ggcgtgataa agagtcgcac accatgcgac tagtcaccat taccgtgctg
    12661 tcgattatcc cgattgcatt gatggtgctg actgccacgg gttatttcta caccacgctg
    12721 cgcctctccg ggcgttggat tgaaaccgtt tatctggtga tcatctggaa cttgctctac
    12781 cagacggtat tgcgcggctt aagcgtagcg gcgcggcgta tcgcctggcg tcgtgcgctg
    12841 gcgcgtcggc agaatctggt gaaagagggc gcagaaggtg ccgaaccgcc ggaagaaccc
    12901 accattgcac tggagcaagt taaccagcag acgctgcgta ttaccatgtt gctgatgttt
    12961 gcgctgttcg gtgtcatgtt ctgggcaatt tggtccgatt tgatcaccgt gttcagctat
    13021 ctcgacagca tcacgctctg gcattacaac ggcactgaag ctggcgctgc ggtggtgaaa
    13081 aacgtcacca tgggcagtct gttgtttgcg attatcgcct caatggtggc ctgggcgttg
    13141 attcgcaacc tgcctggttt actggaagtg ctggtgctct cgcgactgaa tatgcgccag
    13201 ggcgcgtcgt atgccattac taccatcctt aactacatca ttattgccgt tggtgcgatg
    13261 acggtgttcg gatcgctggg cgtctcttgg gataaactcc agtggctggc cgcagcatta
    13321 tccgtaggtc ttggttttgg tttacaagaa attttcggta acttcgtctc cggtttgatc
    13381 attctgttcg aacgtccggt gcgtattggc gatacggtaa ccattggtag cttctcgggg
    13441 acggtaagta agatccgtat tcgtgcgaca acgattaccg atttcgatcg caaagaagtg
    13501 atcatcccga acaaagcgtt tgttaccgag cgtctgatca actggtcgtt gactgacact
    13561 actacgcgtc tggtgatccg tctcggcgtg gcctatggct ccgatctgga aaaagtgcgt
    13621 aaagtgttac tgaaggcggc gactgagcac ccaagggtga tgcacgaacc aatgccggaa
    13681 gtcttcttta cggcatttgg tgccagcacg ttggatcatg agctgcgtct gtatgtgcgt
    13741 gaactgcgtg accgtagccg tactgtcgat gagctgaacc gtactatcga tcagctgtgc
    13801 cgtgaaaacg acatcaacat tgcctttaac cagcttgaag tgcatctgca caacgagaag
    13861 ggcgatgagg tgacggaagt aaaacgcgac tacaaaggcg acgatccgac accagcggtt
    13921 gggtaataac caaagggggc gacatttagt tgccccgaga ttactgacaa agtgcgcgtt
    13981 gttcatgccg gatgcggcgt gaccgcctta tccggcctac gaaaccgcaa gaattcaata
    14041 tattgcagga gcggtgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgccc
    14101 gcaatcttag gggacattta gcggccccat ttatttctca cttttccgcc tcatcatcgt
    14161 gcgttaattt cttttcataa tcacgcttta caatatccag cgcgcgcagc acggtactgg
    14221 cagggatctg attttcctcc agcagcacaa tcaaatcgac agccagtttg acatcgtcag
    14281 gggcattttc aagtgacata ctctttccat tgctagcggg ttaagcgcgc tagcctgttt
    14341 tcgatttttt ccagcgcatg gcggcagcgt gccaggcgcg cttcataggc ttccacttca
    14401 cgatgcagcg tttgctgttc cacgagatcg gtcacccgcg ccaggcgggc tctgcgttcg
    14461 gcaaccatct cacgtagccg ccgctcaaaa tcctgatgct gaatacgttt acgctgccag
    14521 cgggaaattt tcggtggcgc actgtcccac tcgcgcaatg accaggcgga ggcttcacgc
    14581 gcgatggctt ccagttgtgc cgccagatgt tccgccagcc aggccacttg cggcagctgt
    14641 tgctgctcaa ctgcatgacg aagcgcagcc agattatcgc ccgcctcgtc gagacaagac
    14701 tgtagtgttg tcgcacgagt ctgaaaaaga tgcctgtcga aacgagcact tagcgtggcg
    14761 aactgtgaca ccggggcaca acgctgacgc agcgtagcga gctgtccttc cagtttttcc
    14821 agcagcaggg cggttttcac gataagacct caatgaaaat gataattgtt atgctaaagt
    14881 agccactatg taagctgaca acactcaata tgcaacgaat cattttaatc atcattggct
    14941 ggctggcggt agtgctgggt acgctgggcg tggtattacc ggtattaccg acgacgccgt
    15001 ttatcctgct ggcggcctgg tgctttgccc gttcttcccc gcgctttcac gcctggttgc
    15061 tgtaccgctc atggtttggc agctatctac gtttctggca gaaacatcat gcgatgccgc
    15121 gcggcgtcaa accgcgggcg attttgctta ttttgctcac gtttgccatt tctctgtggt
    15181 tcgtccagat gccatgggtg cgcatcatgt tgctggtaat tctcgcctgt ttgcttttct
    15241 atatgtggcg aattccggtg attgatgaaa agcaagaaaa gcactgaagc acaacaatcg
    15301 cagttgcaat tattgcgtac agccagtaca ttctggcgtt ttcgagcaca ggcgcaggcg
    15361 gtcaaaggtt aaacaacagt tacttttgat acgtttaaaa cgcgccgtga gtaccaccgt
    15421 aacaagcagg catacactta tgaccgcgac tgcacagcag cttgagtatc tcaaaaatag
    15481 catcaaaagc attcaggact acccaaaacc cggcattctt ttccgcgatg tcaccagctt
    15541 actggaagac ccgaaagctt acgctctcag catcgacttg ctggttgagc gttacaaaaa
    15601 tgcgggcatt accaaagttg tcggcaccga agcgcgtggc ttcttgtttg gcgctccggt
    15661 agctctgggt ctgggcgttg gctttgtacc ggtccgtaaa ccgggcaaac tgccgcgtga
    15721 aaccatcagt gaaaattacg acctggaata cggcaccgat cagctggaaa tccacgttga
    15781 tgccatcaaa ccgggcgaca aagttctggt ggtggacgat ctgctggcaa ccggcggcac
    15841 tatcgaagcg accgttaaac tgatccgtcg tctgggtggt gaagtggctg acgctgcgtt
    15901 cattatcaac ctgtttgatc tcggcggcga acagcgtctc gaaaaacagg gcattaccag
    15961 ctacagcctt gtcccgttcc cgggccatta attatcgcca gtcttgtgct gcccacgcta
    16021 cggacagcac aagatgtgca ttcagcctcg ccgttctgac ggggctgtgt tagcattacc
    16081 ccttcgtgaa tccaccttcc agcgtttcag agcctgccaa tgagttatca ggttttagcc
    16141 cgaaaatggc gcccacaaac ctttgctgac gtcgtcggcc aggaacatgt gctgaccgca
    16201 ctggcgaacg gcttgtcgtt agggcgtatt catcatgctt atcttttttc cggcacccgt
    16261 ggcgtcggaa aaacctctat cgcccgactg ctggcgaagg ggctaaactg cgaaaccggc
    16321 attaccgcga cgccgtgcgg cgtgtgcgat aactgtcgtg aaatcgagca ggggcgcttt
    16381 gtcgatctga ttgaaatcga cgccgcctcg cgcaccaaag ttgaagatac ccgcgacctg
    16441 ctggataacg tccagtacgc tccggcgcgt ggtcgtttca aagtttatct gatcgacgaa
    16501 gtgcatatgc tgtcgcgcca cagctttaac gcactgttaa aaacccttga agagccgccg
    16561 gagcacgtta agtttctgct ggcgacgacc gatccacaga aattgccggt gacgattttg
    16621 tcacgctgtc tgcaatttca tctcaaggcg ctggatgtcg agcaaattcg ccatcagctt
    16681 gagcacatcc tcaatgaaga acatatcgct cacgagccgc gggcgctgca attgttggcg
    16741 cgcgccgctg aaggcagcct gcgagatgcc ttaagtctga ccgaccaggc gattgccagc
    16801 ggtgacggcc aggtttcaac ccaggcggtc agtgcgatgc tgggtacgct tgacgacgat
    16861 caggcgctgt cgctggttga agcgatggtc gagggcaacg gcgagcgcgt aatggcactg
    16921 attaatgaag ccgctgcccg tggtatcgag tgggaagcgt tgctggtgga aatgctcggc
    16981 ttgttgcatc gtattgcgat ggtacaactt tcgcctgctg cacttggcaa cgacatggcc
    17041 gccatcgagc tgcggatgcg tgaactggcg cgcaccatac cgccgacgga tattcagctt
    17101 tactatcaga cgctgttgat tggtcgcaaa gaattaccgt atgcgccgga ccgccgcatg
    17161 ggcgttgaga tgacgctgct gcgcgcgctg gcgttccatc cgcgtatgcc gctgcctgag
    17221 ccagaagtgc cacgccagtc ctttgcaccc gtcgcgccaa cggcagtaat gacgccaacc
    17281 ctggtgccgc cgcaatcagc gccgcagcag gcaccgactg taccgctccc ggaaaccacc
    17341 agccaggtgc tggcggcgcg ccagcagttg cagcgcgtgc agggagcaac caaagcaaaa
    17401 aagagtgaac cggcagccgc tacccgcgcg cggccggtga ataacgctgc gctggaaaga
    17461 ctggcttcgg tcaccgatcg cgttcaggcg cgtccggtgc catcggcgct ggaaaaagcg
    17521 ccagccaaaa aagaagcgta tcgctggaag gcgaccactc cggtgatgca gcaaaaagaa
    17581 gtggtcgcca cgccgaaggc gctgaaaaaa gcgctggaac atgaaaaaac gccggaactg
    17641 gcggtgaagc tagcggcaga agccattgag cgcgacccgt gggcggcaca ggtgagccaa
    17701 ctttcgctac caaaactggt cgaacaggtg gcgttaaatg cctggaaaga ggagagcgac
    17761 aacgcagtat gtctgcattt gcgctcctct cagcggcatt tgaacaaccg cggtgcacag
    17821 caaaaactgg ctgaagcgtt gagcatgtta aaaggttcaa cggttgaact gactatcgtt
    17881 gaagatgata atcccgcggt gcgtacgccg ctggagtggc gtcaggcgat atacgaagaa
    17941 aaacttgcgc aggcgcgcga gtccattatt gcggataata atattcagac cctgcgtcgg
    18001 tttttcgatg cggagctgga tgaagaaagt atccgcccca tttgatcgta agcacagctt
    18061 acgttcgtca tccttaacgt gattgagaga gaaacctatg tttggtaaag gcggtctggg
    18121 taacctgatg aagcaagccc agcagatgca agaaaaaatg cagaaaatgc aggaagagat
    18181 cgcgcagctg gaagttaccg gcgaatctgg cgcaggtctg gtaaaagtga ccatcaacgg
    18241 tgcacacaac tgccgtcgcg tagagatcga cccgagcctg ctggaagacg acaaagagat
    18301 gctggaagac ctggtggctg cagcattcaa cgacgcagca cgtcgtattg aagaaacgca
    18361 gaaagaaaaa atggcctctg tatcctccgg aatgcagctg ccgcctggct ttaagatgcc
    18421 gttctgatgc aaaccagccc gctgttaaca cagcttatgg aagcactgcg ctgtctgccg
    18481 ggcgttggcc cgaagtcggc gcagcgtatg gcgttcacgc tgcttcagcg cgatcgtagc
    18541 ggcgggatgc gtctggcgca ggcgctcacc cgggcgatgt cggaaatcgg ccactgcgcc
    18601 gattgccgca ctttcaccga acaggaagtc tgtaacatct gttcgaatcc gcgtcgtcag
    18661 gaaaacggtc aaatctgtgt ggtggagagt ccggcggaca tctacgccat tgagcagacg
    18721 gggcagtttt ctggtcgtta ttttgtgttg atggggcatc tgtcaccgct ggacggcatc
    18781 ggtccggatg atatcgggct tgatcgtctg gaacagcgtc tggcagagga aaaaatcact
    18841 gaagtgatcc tcgccaccaa tcccacggtt gaaggtgaag ctaccgctaa ctacattgcc
    18901 gagctttgcg cgcaatatga cgtggaagcc agccgaatcg ctcatggcgt accggttggc
    18961 ggcgaactgg aaatggtcga tggcaccacg ctgtcacact ccctcgccgg gcgtcataag
    19021 attcgttttt aagcaaacga gagcaggagc acctgctctc gcttgaaatt attctccctt
    19081 gtccccatct ctccctcatc ctgtttttaa ccttaaaatg gcattattga ggtagaccta
    19141 catgaaagga caagaaactc gtggttttca gtcagaagtg aaacagcttc tgcacctgat
    19201 gatccattct ctctattcca ataaagaaat cttcctgcgt gagcttatct ctaacgcctc
    19261 cgatgcggcg gacaagctgc gtttccgtgc gctatctaac ccggacctgt acgaaggtga
    19321 tggcgaactg cgcgttcgtg tctctttcga taaagacaag cgcacgctga ccatctccga
    19381 taacggcgtg gggatgaccc gcgacgaagt gattgaccat ctggggacta tcgctaaatc
    19441 cggtaccaaa tcattcctcg aatccctggg ttctgaccaa gcgaaagaca gccagctgat
    19501 cggtcagttt ggtgttggtt tctactctgc gtttatcgtg gccgacaaag tgaccgtgcg
    19561 tactcgcgcg gcaggcgaaa aaccagaaaa cggcgtcttc tgggaatcgg ctggcgaagg
    19621 tgaatatacc gttgccgata tcaccaaaga agatcgtggt actgaaatca ccctgcatct
    19681 gcgtgaaggc gaagacgagt tcctcgatga ctggcgcgtg cgttccatca tcagcaaata
    19741 ctccgaccat atcgcgctgc cggtagagat cgaaaaacgc gaagagaaag atggcgaaac
    19801 cgttatctcc tgggcaaaaa tcaacaaagc gcaggcgctg tggactcgta acaagtcgga
    19861 aatcaccgat gaagagtata aagagttcta caaacacatc gcccacgact ttaacgatcc
    19921 gctgacctgg agccacaacc gcgttgaagg taagcaggag tacaccagcc tgctgtacat
    19981 cccgtcccag gctccgtggg atatgtggaa ccgtgatcat aaacacggcc tgaaactgta
    20041 cgttcaacgc gtgtttatca tggacgacgc tgaacagttc atgccgaact atctgcgctt
    20101 cgtgcgtggt ctgattgact ccagcgatct gccgctgaac gtttcccgtg aaatcctcca
    20161 ggacagcacg gtaacgcgta acctgcgcaa tgcgctgacc aagcgtgtgc tgcaaatgct
    20221 ggaaaaactg gcgaaagacg acgcggaaaa ataccagacc ttctggcaac agtttggcct
    20281 ggtactgaaa gaaggtccgg cggaagattt cgctaaccag gaagcgatcg ccaaactgct
    20341 gcgttttgct tctacttata ccgattcttc tgcgcagacc gtgtctctgg aagactacgt
    20401 ttcccgcatg aaagaagggc aggagaaaat ctactacatc actgcagaca gctatgcggc
    20461 agcgaagagc agcccgcacc tggaactgct gcgtaagaaa ggcatcgaag ttctgctgct
    20521 ttccgaccgc atcgatgagt ggatgatgaa ctatctgact gagttcgacg gtaaaccgtt
    20581 ccagtcggtg tctaaagttg acgagtcgct ggaaaaactg gctgacgaag ttgatgagag
    20641 cgcgaaagaa gcggagaaag cactgactcc gttcatcgac cgcgtgaaag ctctgctcgg
    20701 cgagcgcgtg aaagatgtcc gtctgactca ccgtctgacc gatacgccag cgatcgtttc
    20761 gaccgacgcg gacgaaatga gcactcagat ggcgaaactg ttcgctgcgg cgggccagaa
    20821 agtgccagaa gtgaaataca tcttcgaact gaacccggat cacgtactgg tgaaacgtgc
    20881 ggcagatact gaagatgaag ccaagttcag cgagtgggta gaactgctgc tggatcaggc
    20941 gctgctggca gaacgcggca cgctggaaga tccgaacctg tttattcgtc gtatgaatca
    21001 gctgctggtt tcctgatgtc atgccggatg acctttgtgt catccggcat ttttcttttc
    21061 atcatctgta ctttccgcaa attatctcgc cattaaccgt ttcagcctca ggtgcctttc
    21121 ttgaggcaat cgcctgttgg tggtatcgtt tatcgctttt tcaaaaaaat cgacacattt
    21181 taaggggatt ttcgcaatgc gtatcattct gcttggcgct ccgggcgcgg ggaaagggac
    21241 tcaggctcag ttcatcatgg agaaatatgg tattccgcaa atctccactg gcgatatgct
    21301 gcgtgctgcg gtcaaatctg gctccgagct gggtaaacaa gcaaaagaca ttatggatgc
    21361 tggcaaactg gtcaccgacg aactggtgat cgcgctggtt aaagagcgca ttgctcagga
    21421 agactgccgt aatggtttcc tgttggacgg cttcccgcgt accattccgc aggcagacgc
    21481 gatgaaagaa gcgggcatca atgttgatta cgttctggaa ttcgacgtac cggacgaact
    21541 gattgttgat cgtatcgtag gccgccgcgt tcatgcgccg tctggtcgtg tttatcacgt
    21601 taaattcaat ccgccgaaag tagaaggcaa agacgacgtt accggtgaag aactgactac
    21661 ccgtaaagac gatcaggaag aaaccgtgcg taaacgtctg gttgaatacc atcagatgac
    21721 tgcaccgctg atcggctact actccaaaga agcggaagcg ggtaacacca aatacgcgaa
    21781 agttgacggt accaagcagg ttgctgaagt tcgcgctgat ctggaaaaaa tcctcggcta
    21841 atcaagccgc agcagatctg ctttcgggca ggtctgctta tccacctcag gcaatacctc
    21901 tcacagcaat tagttcttct tcctcacttt tccgctacaa ttatcaacaa gttgaatcga
    21961 taagaggcgg taatgcgtca gactaaaacc ggtatcctgc tggcaaacct gggtacgccc
    22021 gatgccccca cacctgaagc ggtaaaacgc tatctgaaac aatttttaag cgacagacgc
    22081 gtggttgata cctcacggtt gttatggtgg ccgttgctgc gcggcgtgat tttgccgctg
    22141 cgctcgccgc gtgtggcgaa gctgtacgcc tctgtctgga tggaagacgg ctcgccgctg
    22201 atggtttaca gccgccagca acagcaggcg ttggcacaac gtttaccgga tacgccagtg
    22261 gcgctgggca tgagctacgg ctcgccatca ctggaaagct ccgtcgatga actcctggca
    22321 gagcatgtag atcatattgt ggtgctgccg ctttatccgc aattctcctg ttctacggtc
    22381 ggtgcggtat gggatgaact ggcacgcatt ctggcgcgca aacgtagcat tccggggata
    22441 tcgtttattc gtgattacgc cgataaccac gattacatta atgcactggc gaacagcgta
    22501 cgcgcttctt ttgccaaaca tggcgaaccg gatctgctgc tgctctctta tcatggcatt
    22561 ccccagcgtt atgcagatga aggcgatgat tacccgcaac gttgccgcac aacgactcgc
    22621 gaactggctt ccgcactggg gatggtaccg gaaaaagtga tgatgacctt tcagtcgcgc
    22681 tttggccggg aaccctggct gatgccttat accgacgaaa cgctgaaaat gctcggagaa
    22741 aaaggcgtag gtcatataca ggtgatgtgc ccgggctttg ctgcggattg tctggagacg
    22801 ctggaagaga ttgccgagca aaaccgtgag gtcttcctcg gtgcgggcgg gaaaaaatat
    22861 gaatatattc cagcgcttaa tgccacgccg gaacatatcg aaatgatggc taatcttgtt
    22921 gccgcgtatc gctaaagctg agcggtaaag aactgagcgc cgtcgcgaag agcctcgtcg
    22981 gcggttttca tcatccgtga ataatgcaaa aaggcatgca gcgtacctgg gtagagtttg
    23041 aactcacagg gctgctgatg cgccgctaac gtctagtaaa gcagacggct gtcatccagc
    23101 agcggatcga actccgcccc ggcaataaaa cagggcggaa cttcgcgagt gagatcatta
    23161 ttaaacagac agtaatacgg tgactcgcgg tccgcgtcgt tgcttaaata cgcctcttca
    23221 tacatctgca aatcctgttg cgttaagcca tcccagacac cgcccaacag acgacgagtc
    23281 acggaatccc gtaatccgta aagcccatac cacagcaaaa cacccgcaat tttaccgcaa
    23341 tcgatctgtt tatcacgcaa ccacaacgca ctggcgagcg ccagcatggc acctgcggaa
    23401 tcaccggcaa agccaatgcg ggacatattg atttgataat cctccgcctg ctggtggaaa
    23461 taacaacaag cagccacaat ttcctctatc gcttgtggaa aacgcgcttc aggtgaaagg
    23521 gtgtaattaa taccaatcac cgtacattgg ctgtagcttg ccagcaggcg catgatgcga
    23581 tcgtgggtat cgagattgcc gagaataaaa ccgcctccat gcaaataaaa tagcgtcgcc
    23641 gggctatctg gctgcggaca aaagagacgt gtttccacct gcccatattt tgttggaacc
    23701 atgtaagctc tggttgccat ttctggagcg cccgcattcc agaatcggcg ctcaagcgtg
    23761 taatactgtc gttgctcagc aatcgttccc gttgcgggcc aggacggtaa atccggctga
    23821 agagtattca caacggtctt catttcagca gaaataaggt ccagaacagg tagtttgttt
    23881 tccggcttca tacaaaaact cctttcaaat tacgtcattg taaggaacca ctgccatgaa
    23941 aatgcgatcc cgcctgctga tattgaaact ggctgcgtct cgcgcgctcc cgtcagattg
    24001 tgttaacatt cgccgctcag ttaaccaccc gtaaaaacaa ccatgaaatt tcccggtaaa
    24061 cgtaaatcca aacattactt ccctgtaaat gcacgcgatc cgctgcttca gcaattccag
    24121 ccagaaaacg aaaccagcgc cgcctgggtc gtaggtattg accagacgct ggtcgatatt
    24181 gaagcgaaag tggatgatga attcattgag cgttatggat taagcgccgg gcattcactg
    24241 gtgattgagg atgacgtagc cgaagtgctt tatcaggagc taaaacagaa aaacctgatt
    24301 acccatcagt ttgcgggtgg caccattggt aacaccatgc acaactactc ggtgctcgcg
    24361 gacgaccgtt cggtgctgct gggcgtcatg tgcagcaata ttgaaattgg cagttatgcc
    24421 tatcgttacc tgtgtaacac ttccagccgt accgatctta actatctaca aggcgtggat
    24481 ggtccgattg gtcgttgctt tacgctgatt ggcgagtccg gggaacgtac ctttgctatc
    24541 agtccaggcc acatgaacca gctgcgggct gaaagcattc cggaagatgt gattgccgga
    24601 gcctcggcac tggttctcac ctcatatctg gtgcgttgca agccgggtga acccatgccg
    24661 gaagcaacca tgaaagccat tgagtacgcg aagaaatata acgtaccggt ggtgctgacg
    24721 ctgggcacca agtttgtcat tgccgagaat ccgcagtggt ggcagcaatt cctcaaagac
    24781 cacgtttcta tccttgcgat gaacgaagat gaagccgaag cgttgaccgg agaaagcgat
    24841 ccgttgttgg catctgacaa ggcgctggac tgggtagagc tggtgctgtg caccgccggg
    24901 ccaatcggct tgtatatggc gggctttacc gaagacgaag cgaaacgtaa aacccagcat
    24961 ccgctgctgc cgggcgctat agcggaattc aaccagtatg agtttagccg cgccatgcgc
    25021 cacaaggatt gccagaatcc gctgcgtgtc tattcgcaca ttgcgccgta catgggcggg
    25081 ccggaaaaaa tcatgaacac taatggagcg ggggatggcg cattggcagc gttgctgcat
    25141 gacattaccg ccaacagcta ccatcgtagc aacgtaccaa actccagcaa acataaattc
    25201 acctggttaa cttattcatc gttagcgcag gtgtgtaaat atgctaaccg tgtgagctat
    25261 caggtactga accagcattc acctcgttta acgcgcggct tgccggagcg tgaagacagc
    25321 caggaagagt cttactggga tcgttaagtt atcgtcggtt cgtaggccag ataaggcgtt
    25381 cacgccgcat ctggcatttg gctctcgatg cctgatgcga cgctggcgcg tcttatcatg
    25441 cctacatatt tttcatattt tacatccggc aaccaccgtt taccccgtca ccacttcacc
    25501 cgccggtggc gtttccagca gttccagcat agtacgggca atttcacgtt cacccatcac
    25561 tacctgattc gcgccacgtt cggtgatata cgtcacttca tcgtcataat gggcgcgggc
    25621 gataatctca atatccgggt ttttcgcgcg ggcagacgcc acaatctcac ccgcttcata
    25681 accgttgggg atcgtcagga tcagccattt tgcacattcc agatgtgcca gttgcataat
    25741 ttcttcgttc gctgcattgc ccaatacagc gcggacccca cgctctcgca gctcatcaac
    25801 acgggttcgt gacgtctcaa tcaccaccag cggaatatca gaggcgagca atttctcccc
    25861 cagcaggctg cctacacgac cgtaacccac caatagcgca tggttgcaaa tatccactgg
    25921 gatctgtttc tcttcttcga ttgcctcttc cagcgtctgc tcttccagcg tttcggtctt
    25981 cgccagatat ttctccagta gtgcgaacag taccgggttg agcataatcg acaggatcgc
    26041 ccctgccagt accaggtttt gtccggcctg cggcagtaaa ttcaatgcca ttcccagtcc
    26101 cgccaggata aacgcgaact caccaatctg cgccaggctg gcggcgatgg ttaatgccgt
    26161 acgttgggag tgaccaaaca gacggacaag gaacagggcc gcgaccgatt taccaaacag
    26221 aataattgcc agcgtcgcca gcactgccag cggttgctga atcagaatta acggatcaaa
    26281 caacatcccg actgagacaa aaaacagcac cgcaaacgcg tcgcgcaatg gcaacgtatc
    26341 gtgggcggca cggtgactca attcagactc gttcagtacc atcccggcaa agaacgcacc
    26401 gagtgcaaag gagacatcaa acagctctac cgcaccaaag gcaatcccta acgccagcgc
    26461 cagcactgac agggtaaaca gctcgcgaga accggttgcc gcgctgcgtg ccataatcca
    26521 cggcaccaga cggcgaccta ccagcatcat aatggcgata aatgcgatca ccttgccgat
    26581 ggtgatcccc atatcgactg caagagtggc aaagcccaca tcgccctgtt ccatcattcc
    26641 tgccactgcg ggcagcaaca ccagcgtcag aaccatcacc aggtcttcca caatcaacca
    26701 accgatggcg atttgcccac gctgactgtc tattaattgc cgttcttcaa gtgcgcgcag
    26761 taacaccacg gtactggcgg tggaaagaca taagccgaac acgataccgg tcattaacga
    26821 ccagcccagc acggcggaga gcgccatacc cagcagcgtc gccacggcta tctgggcgat
    26881 cgcgccggga atggcgatgg actttaccgc catcaaatcc ttgagcgaaa aatgcaaacc
    26941 gacgccgaac atcagcaaaa taacgcccag ttcagccagt tccggcgcaa gcttggtatc
    27001 ggcaacaaag cccggagtga atggtcctgc cagcacaccc gctaacagat atcccaccag
    27061 aggagaaata cgtagtttat tggccagcat gccgaggata aaggcgagca caaggccgcc
    27121 aacaatggtg gtgataagcg gggtggcgtg atgcattccg tctccttttc ctggtggtta
    27181 ttgtccattt ttggctggga aaaccaaaat tacaggtaat agtttatgac aatttcattg
    27241 atgatgttta tgaataattg ttgaattttg cagaaaaatg gaattagctg caaaaaaagc
    27301 acggttcgga aaacagaagg cggtttaaca gaggaaaggc ttatggcgac tgtaaaacga
    27361 tgcagccaaa gtttgccttt ggctgcaatg aaatcagtct ttatgccggt tatcaggcag
    27421 gaatatggtc aacatcccca atagtggcag gaaagcacag attttataga ctaactcgat
    27481 gctggtgtga tcggcgataa gccccagaac tgccgctccc agacctccca tgccgaaagc
    27541 aaaaccgaaa aagagtccag aaaccatacc gatacgtccc ggaagtagct cctgagcgta
    27601 gaccagaatg gcagagaatg ccgaagcgag gataaatcca ataatcaccg ttaaaacccc
    27661 cgtccagtgc aggcgggcgt agggtaaaat cagcgtaaac ggcgcaacgc cgaggataga
    27721 gccccaaatc acatatttcc gtccaatttt atcccctaca ggcccgccga tcaccgtacc
    27781 tgccgcaacg gcaaacagga aggcaaacag atgaagctgg gcattctgga tagataatcc
    27841 gaatttttgc atcagataaa aggtgtaata gctgctgatg ctcgccatat agaaatattt
    27901 cgagaaaatg aggattaaca gaatgctgac tgccagtacc actttgttgc gcggcagtgg
    27961 attgattatc gtcgctttgg gttttccttt attcattcgg tgctgtgccg agtaccaacg
    28021 gctaatttgc gccaacacca cgatcgccag cagtgccgca agcacaaacc aggcaacgtt
    28081 gcctttgcca taaggctcga taatcaccgc cgccagcaag ggtcccaggg aactgccaaa
    28141 gttgccgccg acctgaaaga tagattgcgc caggccatgc cgcccgccgg aagccatacg
    28201 ggctacgcga gaagattccg gatgaaagac cgatgaaccg gtaccgacca gcgccgctgc
    28261 cagcagaact gcgccaaaac tgcccgccag cgcaagcagc accagaccac ttaaggtaaa
    28321 gcacatgcca attggcaacg accacggcat cggatattta tcggtccagt agccgaccac
    28381 tggttgcagt agcgaagagg cgagctggaa ggtgagggtt atcatgccaa tctgcataaa
    28441 tgtcagagaa aattctgact gaagcagcgg ataaatcgcc agaatcagcg attggatcat
    28501 gtcgttcagc agatgtgaga ggctgatagc acctaaaata ccaaacgatg ttcgggcctt
    28561 ggtcgttgac gcagccgcgc ccgccacagg ctgggtttgt tcactcattg ccataggaaa
    28621 gtcacttttt cagggttgcg atgtaaagaa tgatcttatt tgtgattatt acccgactaa
    28681 catacctgta tgcgtcgtct gaaggaagtc tcaacgccga atacagaatt tctaatctgg
    28741 atgcagattt atcttcaccg gacgcagact tgtctatgat gttgcgtcat actatttttc
    28801 aacacgttga aatcaggtca gggagagaag tatgaaatta ttgcagcggg gcgtggcgtt
    28861 agcgctgtta accacattta cactggcgag tgaaactgct ctggcgtatg agcaggataa
    28921 aacctacaaa attacagttc tgcataccaa tgatcatcat gggcattttt ggcgcaatga
    28981 atatggcgaa tatggtctgg cggcgcaaaa aacgctggtg gatggtatcc gcaaagaggt
    29041 tgcggctgaa ggcggtagcg tgctgctact ttccggtggc gacattaaca ctggcgtgcc
    29101 cgagtctgac ttacaggatg ccgaacctga ttttcgcggt atgaatctgg tgggctatga
    29161 cgcgatggcg atcggtaatc atgaatttga taatccgctc accgtattac gccagcagga
    29221 aaagtgggcc aagttcccgt tgctttccgc gaatatctac cagaaaagta ctggcgagcg
    29281 cctgtttaaa ccgtgggcgc tgtttaagcg tcaggatctg aaaattgccg ttattgggct
    29341 gacaaccgat gacacagcaa aaattggtaa cctggaatac ttcactgata tcgaatttcg
    29401 taagcccgcc gatgaagcga agctggtgat tcaggagctg caacagacag aaaagccaga
    29461 cattattatc gcggcgaccc atatggggca ttacgataat ggtgagcacg gctctaacgc
    29521 accgggcgat gtggagatgg cacgcgcgct gcctgccgga tcgctggcga tgatcgtcgg
    29581 tggtcactcg caagatccgg tctgcatggc ggcagaaaac aaaaaacagg tcgattacgt
    29641 gccgggtacg ccatgcaaac cagatcaaca aaacggcatc tggattgtgc aggcgcatga
    29701 gtggggcaaa tacgtgggac gggctgattt tgagtttcgt aatggcgaaa tgaaaatggt
    29761 taactaccag ctgattccgg tgaacctgaa gaagaaagtg acctgggaag acgggaaaag
    29821 cgagcgcgtg ctttacactc ctgaaatcgc tgaaaaccag caaatgatct cgctgttatc
    29881 accgttccag aacaaaggca aagcgcagct ggaagtgaaa ataggcaaaa ccaatggtcg
    29941 tctggaaggc gatcgtgaca aagtgcgttt tgtacagacc aatatggggc ggttgattct
    30001 ggcagcccaa atggatcgca ctggtgccga ctttgcggtg atgagcggag gcggaattcg
    30061 tgattctatc gaagcaggcg atatcagcta taaaaacgtg ctgaaagtgc agccattcgg
    30121 caatgtggtg gtgtatgccg acatgaccgg taaagaggtg attgattacc tgaccgccgt
    30181 cgcgcagatg aagccagatt caggtgccta cccgcaattt gccaacgtta gctttgtggc
    30241 gaaagacggc aaactgaacg accttaaaat caaaggcgaa ccggtcgatc cggcgaaaac
    30301 ttaccgtatg gcgacattaa acttcaatgc caccggcggt gatggatatc cgcgccttga
    30361 taacaaaccg ggctatgtga ataccggctt tattgatgcc gaagtgctga aagcgtatat
    30421 ccagaaaagc tcgccgctgg atgtgagtgt ttatgaaccg aaaggtgagg tgagctggca
    30481 gtaatccgaa agtgccggat gtttgcatcc ggcacaatgc ttaatcgcgg cgggcgatat
    30541 cagcaaattt ggcatcgagg atctttgcca gatcgcctgc cgccagttcg atatccagtc
    30601 cgcgcttgcc gccggaaaca taaatagtgg caaattcttg tgcgggggcg tcgataatcg
    30661 ttggcagacg ttttttctgc cccagtgggc taatcccccc aaccaggtat cccgtcgaac
    30721 gctgcgcgac catcggatcg gccatctcaa ctttcttggc acccagcgct tttgctactt
    30781 ttttaagatc cagttgacct gcgaccggcg taacggccac ggcaaggtgt ttcatatcac
    30841 cgttcactgc caccagcagc gttttgtaga cctgatccgg attcaaacct aattttttga
    30901 cgacttcatc gccaaaattg gtttcagccg gatcgtgctc gtaggtatgg atttgaaacg
    30961 aaatcttgtt tttttcgagt aatttaactg cgggtgtcat ggtaattttc ctgccttaaa
    31021 caaaaaaacg aagcaagcat acgcctgatg gcggtccaaa aaaaagagtc atcttgccta
    31081 agagtattgg caggatggtg agattgagcg acaatcgagt tacaccgtcg cttaaagtga
    31141 cggcataata ataaaaaaat gaaattcctc tttgacgggc caatagcgat attggccatt
    31201 tttttagcgc aacatttgcg gcaaattccc ttctccatac aggtgtagtg caccgaccgc
    31261 gaccacatat cgccccggcg gcatggcgcg taatttatcc cgccaggcga gatttcgctg
    31321 atgcatcagc acatcgtaca gcgactgact gaacgtattg ggcagcgtta tatcattatt
    31381 ttgcggcggt gcattcagcc accagctcat catttgttgc agcaaccgtg cgttggtatg
    31441 ccagtgggtc agcgtatcgt ccagcagcgc cagtcctttg tcagggagct ggagcaacat
    31501 ggcaatctgg ttttcagccc cttccagttc aatcacgggt ttatgttgtt gcttcgccgc
    31561 ctgcaatagc tggtaatcga taccgtattc tgcccgcagc cccagttttt gtgcctgcgt
    31621 cgcctgaaga accatcgcga tttgccacag cggttgggta gaaaagagtg agggagaaat
    31681 gcccatctcc tggctaatgt gctgcaggtt ttggagttgc tcctcgctaa tgcgctcttc
    31741 cagcgcctcg caggcaggca aattagcaaa aggcgtatcg ctggtggaaa catccgcctc
    31801 gacgatcagc gcatcggcgt ttttgagctt tttgagcaaa cgggtgggca ggggagccat
    31861 atcgtggcta cccatatgaa tactgccaat cagatgaaaa tggcgattgc cggggagggt
    31921 gatatcgatg gcaggccagg tgtaatgatt accgcgcagc gcggcccaaa gcgtttttac
    31981 ccggtacaac agatccatac gacctccctt tgtgaaatat catgctagcg cgcggtgagg
    32041 gatggcgcaa atggagttgg ggacttgttt aatgtgtttg tatgattcag tatgttcttg
    32101 catcgctatt cacagggaag caacagttaa aaaccatgaa acaggcaaca agaaaaccga
    32161 cgacacccgg agatattctt ctctatgaat atctggaacc gctcgatttg aaaatcaatg
    32221 agttagcaga gttgctgcat gttcatcgta atcgcgtcag tgcactgatc aataacaatc
    32281 gtaaactcac cactgagatg gcattccgtc tggcgaaagt ttttgatacc acagtcgatt
    32341 ttaggctaaa cctccaggcg gcagttgatc tttgggaagt tgaaaacaac atgcgcaccc
    32401 aggaagaatt gggacggatt gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg
    32461 caaaaaaggt cgcgtaaggc caaaacatcg cttcatctcg taggccggat aaggcgttca
    32521 cgccgcatcc ggcatccgag caccattgcc tgatgcgacg cttacgcgtc ttatcaggcc
    32581 tacaaacctg tgcctgaacc gtaggccgga taaggcgttt acgccgcatc cggcagtcat
    32641 gcgtcgatgc caaatgcgcc gccctaaagc agcgcatccg caatgacgta cttattcctt
    32701 cggtttaaac cgcagcaacc ggttggcgtt acttactacg gtaattgacg agagcgccat
    32761 tgccgctccg gcaactaccg ggttaagcag tgttccagta aacggccaca aaataccggc
    32821 ggcgaccgga ataccgatgc tgttgtagat aaaggcaccg agcaggttct gcttcatgtt
    32881 gcgcagcgtt gcgcgggaaa tcgcgagcgc atccgcaacg cccatcaggc tatggcgcat
    32941 cagggtaatc gccgcggttt caatggcaac atcactgccg ccacccatcg caatgccgac
    33001 atccgcctgg gccagcgctg gcgcgtcgtt aatgccgtcg cccaccattg ccacctgacg
    33061 tccttcactt tgcagacgtt tgatcgcttc ggctttaccg tccggcagca ctccggcaat
    33121 cacttcatca attccagctt ctttggcgat cgcattggcg gtggttgggt tatccccggt
    33181 caacatcacc agacgatatc ccgctttatg caggcgttgc agcgccgcca cgctatcact
    33241 acgcaacgga tcgcgtacag ccagcagggc tactgctttc ccgtcaatcg ccagcagcac
    33301 aggcgttgct ccttgcgatg cctgagctga aatatcggct tcaatcgctt tagtatcgac
    33361 ttgctgatca tttaacaacg cctgattacc cagcaataac gcatgacctt ctgcttcacc
    33421 gctcacgccc agcccgcgca atgtgcggaa accgttgacc tgcggtagct gcatatcgcc
    33481 tgctttatca aggatcgctc gtgccaatgg atggctggaa ccttgctcca gcgccgccgc
    33541 cagacgcagc gcctgcgctt cgtcaacatc agcaaatgtt ttcactgcga caacctgcgg
    33601 cttcccttcg gtcagcgtcc cggttttatc gaacacgacg gtatcgagtg tactggcgcg
    33661 ttgcagcgcg tcggcgtccc gcaccagcac gccaaactca gccgcccgcc cgacgccgga
    33721 aataatcgac atcggcgtcg ccagtcccag cgcacacggg caggcgataa tcagcaccgt
    33781 agtggcaatc accagggtat agacaatctg cggtgccgga ccaaagaaat accagattgc
    33841 cgcactgaca agcgcaataa ccactactac cggcacaaat atggctgaga ttttatccgc
    33901 cagctgaccg atttctggct tgctgctctg ggcctggcgc accatgcgaa tgatccttga
    33961 tagcgtggta tggctgccaa ccgcactggc acgaaacagc acactgccgt cctgtaccac
    34021 tgtcccggca tggacgctat cgccttcgcc tttttgctgc gggattggtt cgcccgtcag
    34081 catcgcttca tccagccatg cttcgccctg ggtaatctcg ccatctaccg gcacgcgatc
    34141 gccggtcgtc aggcgcagca acatacctgg ctgcacttct gccagaggca cgcttttttc
    34201 accttcgtca gtaaccaggc gcgccgtcgg tggggttaaa tcgagtaatt tctccagtgc
    34261 cttagaagaa cgctgacgtg cgcgtgcttc cagcatatgg ccgagattga tcagaccgat
    34321 aatcatcgca ctggcttcat agtaaagatg tcgcgcttcc atcgggaacc actgcggcca
    34381 caggttgacg ctcatcgaat agagccacgc cacgcccgtt cccagcgcca ccagcgtatc
    34441 catcgtcgct gcaccgttca gcaggctttt ccatgcactg cggtaaaaat ggccgctggc
    34501 gaaaaccatc accgccaggg ttatcaggcc gataaccaac cacaggctgc ggttgtcggc
    34561 ggtgactatc atgttatcgc cgatcatccc ccagaccatc accgggatac ccaccgccag
    34621 tgcgacaatt gcctgccagc ggaagcgctt catcgtagcg acggcggttt cttgctggcg
    34681 ttcgcggcgt ttcgcgtcat cttcaatcgc ttccgcgccg tagcccgctt tttccaccgc
    34741 ctgcactaaa tcttgtgggg aggcactgcc catcaccagc gcagtacgct ccgccaggtt
    34801 tacccgtgcc tgagtgacgc ccggtacgct ttgcagcgca ttttgtacgc gggtgacaca
    34861 gctggcgcag ctcatgccgc tcagcagcaa ctgctggctg tcatcgtcat cggcggtcgc
    34921 tgccggaagc gcctcagaaa ccgctgtcag tgcttccgac gggattgatg actccgccag
    34981 cggtttagcc tttgggtggc ttacagatgc gtcataaccc gcttgtttga tggtttcgat
    35041 cagctgttct gcactggcag tcccggtaac gtgcgcttca gtgatagaca catccgcttg
    35101 ttccacatcc gggcgctgtt caagactttc tttcacgcgt ttaacgcagt gaccgcagga
    35161 caggccgtcc aggttcaggt cgatagtttg tgacataaaa cactccttta agacagtttt
    35221 gactggctgt gataaaggtt aaaccttcca gcaaggggaa ggtcaagaaa ttaataaacc
    35281 aggcgggtaa aagtccgtaa agattaaaaa atcggctcga tttgcatcag gattagacat
    35341 ttatttcttt gttttcctgt agttaagttg cgggtgctaa gttaaatcag gatgcctgaa
    35401 aatcggcact ggggtgagga attacctccc gcatctataa aaaggagtta acaaaagatg
    35461 ttagatgcaa acaaattaca gcaggcagtg gatcaggctt acacccaatt tcactcactt
    35521 aacggcggac aaaatgccga ttacattccc tttctggcga atgtaccagg tcaactggcg
    35581 gcagtggcta tcgtgacctg cgatggcaac gtctatagtg cgggtgacag tgattaccgc
    35641 tttgcactgg aatccatctc taaagtctgt acgttagccc ttgcgttaga agatgtcggc
    35701 ccgcaggcgg tacaggacaa aattggcgct gacccgaccg gattgccctt taactcagtt
    35761 atcgccttag agttgcatgg cggcaaaccg ctttcgccac tggtaaatgc tggcgctatt
    35821 gccaccacca gcctgattaa cgctgaaaac gccgaacaac gctggcagcg aattttacat
    35881 atccaacagc aactggctgg tgagcaggta gcgctctctg acgaagtcaa ccagtcggaa
    35941 caaacaacca acttccataa tcgggccatt gcctggctgc tgtactccgc cggatatctc
    36001 tattgtgatg caatggaagc ctgtgacgtg tacacccgtc agtgctctac gctcatcaat
    36061 actgttgaac tggcaacgct tggcgcgacg ctggcggcgg gtggtgtgaa tccgttgacg
    36121 cataaacgcg ttcttcaggc cgacaacgtg ccgtacattc tggccgaaat gatgatggaa
    36181 gggctgtatg gtcgctccgg tgactgggcg tatcgcgttg gtttaccggg caaaagcggt
    36241 gtaggtggcg gtattctggc ggtcgtccct ggcgtgatgg gaattgccgc gttctcacca
    36301 ccgctggacg aagaaggcaa tagtgttcgc ggtcaaaaaa tggtggcatc ggtcgctaag
    36361 caactcggct ataacgtgtt taagggctga tcatgatgaa cacggaaggt aataacggta
    36421 acaaacccct cggtctatgg aacgtcgttt ccatcggtat tggggcaatg gtgggggcgg
    36481 ggatcttcgc gctgctgggg caggctgcgt tgctaatgga agcctcgacc tggggcgcct
    36541 ttgcttttgg cggtattgtg gctatgtttt ccggttatgc ctatgcgcgt ctgggggcga
    36601 gctatcccag caatggcggt attatcgact tttttcgtcg cggattaggc aacggcgtct
    36661 tttcgctagc gctctcgttg ctatacctgt tgacgctggc ggtgagcatc gccatggttg
    36721 cccgtgcttt tggcgcttat gccgtgcagt ttttgcatga aggcagccag gaggagcacc
    36781 ttattttgct ctacgcgttg gggatcattg cggtgatgac gcttttcaac tccttaagca
    36841 accatgcggt agggcggctg gaagtgatcc tcgtcggcat taaaatgatg atcctgttat
    36901 tgctgattat tgccggtgtc tggtcgctgc aaccggcgca tatttccgtc tctgcgcccc
    36961 ccagctccgg tgcgttcttc tcctgtattg ggataacctt ccttgcctat gcgggctttg
    37021 gcatgatggc gaacgcggcg gataaagtga aagatccgca ggtcattatg ccacgggcgt
    37081 ttctggtggc gattggcgtt accacgttgc tttatatctc gctggcgctg gttttgctta
    37141 gcgatgtatc ggcattagag ttagaaaaat atgctgatac cgccgtagcg caggctgctt
    37201 ctccgctgct cgggcatgtg ggttatgtga tcgtcgtcat cggcgcttta ctggcgacgg
    37261 cttcaggcat taacgcgaac ctgttcgccg tgtttaacat catggacaac atgggcagcg
    37321 aacgcgaact gccgaagcta atgaataaat ccctgtggcg gcagagtacc tggggcaata
    37381 tcatcgtcgt ggtgttgata atgctgatga cggcggcgct aaatttagac tcactcgcca
    37441 gcgttgccag cgccaccttt ttgatctgct atctggcggt gtttgtggtg gcgatccgcc
    37501 tgcgtcatga tattcacgcc tcgttgccga ttcttatcgt tggtacgttg gtgatgttgt
    37561 tggtgatcgt tggctttatc tacagcctgt ggtcccaggg cagccgtgcg ttgatatgga
    37621 ttattggtgc aatcttactc agccttattg tggcaatggt catgaagcgt aataaaaccg
    37681 tataacacct ctctgtgcgc agtacttcct gtattattgt ggtggcggtc gatattcgca
    37741 ctggcaaaaa aacgtgcttg aatatctgtt gaaacccttt aacaaagcac aggaggcgtt
    37801 gcgcgaacga tgaacatcag cgatgtagca aaaattaccg gcctgaccag caaagccatt
    37861 cgcttctatg aagagaaggg gctggtgacg ccgccgatgc gcagcgaaaa cggttatcgc
    37921 acctacacgc agcagcatct caacgaactg accttactgc gccaggcacg gcaggtgggc
    37981 tttaacctgg aagagagcgg cgagctggtg aatctgttta acgacccgca gcggcacagc
    38041 gccgacgtca aacggcgcac gctggagaag gtggcggaga tcgaacgaca cattgaggag
    38101 ctgcaatcca tgcgcgacca gctgctggca ctggcgaatg cctgccctgg cgatgacagc
    38161 gccgactgcc cgattatcga aaatctctcc ggctgctgtc atcatcgggc agggtgatta
    38221 agacgagacg gctcggatat gtagggttat cccttctatc gcaatgactt caacatgcgt
    38281 acctgcaccg agatcctcgc tggcgctgac aggccatgaa ctgtcaccga cgcgcatatg
    38341 accgcgcccg ttgaccagcg gagattccag cacaaaacgt cggccaatca gctgctgccc
    38401 gcgctggttt aaatgactat cgctgtgctt ttgttcgcgc acccgccgcg acaaccattt
    38461 ccaccacagc caggcggcga gcagcgtcag gatggcaaac attacccctt gccactccca
    38521 acccagcggc accagccaga ccaccaggcc ggtaatcacc gctgccacgc cgctccacaa
    38581 caaataacca tttccgccca gcatctcggc tgccagcagc aacccgccga gactgagcca
    38641 gaaaatatgt ggatgaacga ccattaactc catcatggct gagtccgttt gttggcgctg
    38701 tctttcacca gctcggcaat cccggcaatc gaccccatca ggctgctggc ctctaatggc
    38761 atcatcacta ctttgctgtt actggaggaa ccgatctgct gtaacgcttc ggtgtatttt
    38821 tgcgctacga agtagttcac cgcctgaata tcaccggagg cgatagcttc agacaccatt
    38881 ttggtggcgc gggcttctgc ttcggcggaa cgttcacgcg cttcagcctg taaaaacgcc
    38941 gactgacgtt cgccttccgc tttcaggatt tgcgactgtt tttcaccttc ggctttgagg
    39001 atttccgcct gacggatccc ttccgcttca agaatgtaag cgcgtttggt acgttccgct
    39061 ttcatctgcg cgttcattga agagataagc tctgccggtg ggcgcacgtc gcgaatttca
    39121 atacgggtga ctttaatccc ccacgggttg gtggcttcat cgacaatacg cagcaggcgt
    39181 gagttgatgc tgtcgcgctg agagagcatt tcgtcaagtt ccattgaacc caacacggta
    39241 cggatgttag tcatggtcag gttgatgatc gccagctcca gattgctgac ttcataggca
    39301 gcgcgtggcg cgtcaatcac ctgaataaaa cagactgcgt cgatggtaac gttggcgtta
    39361 tctttcgaga taacttcctg ggaagggata tcgagcactt gctccatcat attgatcttg
    39421 cgaccaatgc gatccataaa cggcaccacc agactgagcc ccggctgtaa cgttttggta
    39481 tagcgaccaa aacgttctac cgtccattga tagccctgcg gcacgatttt gacgcccgcg
    39541 ccgacaatga ccagcgcgac aaaaatgaga atcgggataa agataagcat cggaaaaacc
    39601 tcctgttgta ccgtccataa tcagcaaaat tgctgcttga ttaaacaaat tatacctgat
    39661 tactgaaaga gagttccccc ttattcctgc gaaggataaa ctgtttttag taaaaatcag
    39721 aaaaagggaa cagcgatgca ggaaaatagt cctttgcttc agctacaaaa cgtaggatat
    39781 ctggcgggtg atacgaagat tcttaataac atcaattttt cgctgcgtgc tggcgaattt
    39841 aagttaatta ccggtccttc tggttgtggc aaaagtacgc tgctaaaaat agttgcttca
    39901 ttgatcagcc caaccagcgg aacgttactg tttgaaggtg aggatgtcag cacactaaaa
    39961 ccagaaatct accgccagca agtctcttac tgcgcccaga cgccgacgct gtttggcgat
    40021 acggtatacg ataatctgat ctttccctgg cagatccgtg accagcagcc tgacccggcc
    40081 atttttctcg attttctcga acgcttcgct ttgccggaca gcattttgac gaagaatatc
    40141 gccgagctat ctggtggtga aaaacaacgc atctcattga ttcgtaacct gcaatttatg
    40201 ccgaaggttt tattgctgga tgaaataacc agtgcgctgg atgaaagtaa taaacataac
    40261 gtcaatgaga tgatccatcg ttatgtgcgc gagcaaaata ttgccgtgct gtgggtgaca
    40321 cacgataaag acgaaattaa tcatgcggat aaagtgatta cactgcaacc gcatgccgga
    40381 gaaatgcagg aagcacgcta tgaactcgca taatattact aacgaatcat tagcactggc
    40441 attaatgctg gtggtggtgg caatcttaat tagccataaa gaaaaactgg cgctggagaa
    40501 agatattcta tggagcgtcg ggcgagcgat aattcagctg attattgtcg gctatgtgct
    40561 gaagtatatt ttcagcgtgg atgatgccag cctgacatta ttgatggtgt tatttatctg
    40621 ctttaatgcg gcgtggaacg cgcaaaaacg cagtaaatat attgctaaag cttttatctc
    40681 atcgtttatt gccattacgg tcggggcggg aattaccctg gcggtgctga ttctctcagg
    40741 ggcgattgaa tttatcccga tgcaggtgat ccctgtcgcc gggatgattg ccggtaacgc
    40801 catggtagcg gtggggttgt gttacaacaa tttagggcaa cgggtcatta gcgaacagca
    40861 acagattcag gagaaactga gtcttggcgc gacgccgaag caggcttcag cgatattgat
    40921 tcgcgacagt attcgcgcgg ctttaattcc gacggtcgat tcagcaaaaa cggttggctt
    40981 agtgagttta ccaggaatga tgtccgggct gatatttgcc gggattgatc cggtgaaggc
    41041 gattaaatat cagattatgg tgacctttat gctgctctca accgccagct tgtcgaccat
    41101 tattgcctgc tatttaacct atcgtaagtt ttataattcg cgccaccagt tggtggtgac
    41161 tcaattgaag aagaaatgat gcgatgccgg atgcgcagca tcgcatccgg cgttgtggtt
    41221 catgtgccga acaacggaat atcaatacaa caatgcatac agctggcggc gatacttcga
    41281 cgccagtgca tcacccgtac ccagcgcagc gaggatctcc tggaacgttt tacgcgtctg
    41341 accgtctgcg gcggtgagat ctttacgcag atgcccgaac agcaactcca gcgcctcttc
    41401 attgcgccca acctgatgca gttgcagcgc cagttgcgtc gccagtgcgg catcttctgg
    41461 attctccgcc acctgctgtt gcaactgttg aatttccggc ttatcagccg cctgcttcag
    41521 cagttcgatt tgcgctacca gcccctggta gcgggtgtcc tgatcctgca acggaatggt
    41581 tttcagcacc gcttccgcat cttcagaacg gttcagcgca atcagcgttt ctgccagcag
    41641 caggccgatc tccccgttct gattcgacaa ctgccaggcg tctttcagca atggcagggc
    41701 atcggtgtaa ttgccttcct gcatcagttg catcgcctgc tgcgctttca gctcttcttc
    41761 gcgcggcagc actttatcca gcagggcgcg gatcgcctct tccggttgcg gcccctggaa
    41821 gccatctacc ggttgcccgt tctggaacag atacacggtc ggaatcgcgc gcagaccaaa
    41881 ctgggcggca atcatctgct ccgcgtcgca gtccagcttc gccagaataa attgcccgtt
    41941 gtactgcgcc gcgaggcttt ccaggatcgg ggttaactgc aaacagtgct ggctacgttc
    42001 agaccaaaaa tagaacagca ccggagtggt catcgactgt tcaagaacct gttgcaggtt
    42061 agattcgtta atgttgacaa tattttctac ggacatggag tcgctctctg ttgtcgatat
    42121 tttctttgac atgggggctt aagcgcgcgc ttcaactcac ccctgcaata ttttgtccat
    42181 cacgcgccca ggcagcaggc gctcgagcac cattactgcc caggtcacca gcgttaccgg
    42241 atagcgcatc ttcggcttct cgctaataaa agcatggcgc actttgtcga ccaccgcttc
    42301 cggtcccaac gtaaagcggg cggcgatgcc gggattttcg actggtttat cactttgcgt
    42361 ctggttgacg ttgtcggtga agcgagtacg aatgggaccg ggttcgatca ggctgacttt
    42421 aattccgctg tggcgtagct ccatgcgcag cgcgtctgac cacgcctcca gcgcatattt
    42481 actggccgcg taagcgccac gacccggcgt ggagattaat cccatcaccg atgatgtcat
    42541 cacaatacgc ccttcaccgt gcggtaacat cgcgggtaac aggcgcatgg tgagctggtg
    42601 tgcgccgaaa aagttggcgg aaaactgctg ttccatctgc gcacggctga tggtggaaag
    42661 ggggccatac atgccgaatc cggcattgtt aaagatccca tacagacaat tatcggtcag
    42721 ggcgatcacc tcgtctgctg cgcgattaac actttctggt gaatccagat cgatcaacac
    42781 gccggtaaat cccatgttgt tcatacgctc aacatcattc ggttttcggc aacctgccag
    42841 cacatgaaaa ccctggcgtt ttaattcgag cgcgctttcc aggccaattc cactggaaca
    42901 tccggtaatt aagaccgatt tttgcataac tttacctgtc aggatctccg ttgctttatg
    42961 agtcatgatt tactaaaggc tgcaactgct tcgccatcca gtcggcaata aacggctggg
    43021 cgtcgcggtt gggatgaata ccgtcatcct gcatccattg tggcttgagg tagacctctt
    43081 ccataaaaaa gggcagcagc ggaacatcaa actctttggc gagtttgggg taaatggcgc
    43141 taaaggcttc attataacgg cgaccatagt ttgcaggcag acgtatttgc attaacaatg
    43201 gttcagcgtt ggcggctttg acatcctgca aaatctggcg cagcgtttgc tcggtttgct
    43261 gtggctgaaa accacgcaaa ccatcattgc gcccagttca accagcaccc aacgcggctg
    43321 atgctgtttg agcagagccg gaaggcgcgc cagcccttgt tgcgaggtgt cgccgctgat
    43381 gctggcatta actaccgacg ttttactctg ccacttatca ttcaacaagg caggccaggc
    43441 cgcgctggca gacattcgat acccggcgct caggctatca cccagaatca ataacgtgtc
    43501 cgctgcggcg gcacggaagg ttaacaggac caggaacagg aagggcaaat gccagcggaa
    43561 aacattgttg aagttcatca tcttaagaag tccgtcggtc agggggagca tgaactctcc
    43621 atcctcaccg gagttgagct ggttgtcaaa cgtggcgaga ccatcgcact ggtgggcgag
    43681 tcgggatcgg gtaagtcaac cttgctggcg atcctcgccg ggcttgatga cggcagcagt
    43741 ggcgaagtga gtctggtggg acaaccgcta cataatatgg acgaagaagc gcgggcaaag
    43801 ttgcgcgcga agcacgtcgg ctttgttttt cagttattta tgttaattcc tacccttaac
    43861 gcgctggaaa acgtcgagct tccggctctg ctgcgcggtg agagtagcgc ggaaagtcgt
    43921 aacggggcga aagcgttgct cgaacagtta gggctgggta aacgtctgga tcatcttccg
    43981 gcacagcttt ccggcggtga acagcaacga gtggcgctgg cacgagcctt taatggtcga
    44041 cctgatgtgc tgtttgccga cgaacccacc ggcaaccttg accgccagac gggcgataaa
    44101 attgccgacc tgctgttttc cctcaaccgt gaacatggca ccacgttgat tatggtgacc
    44161 cacgacctgc aactggcggc acgctgcgac cgctgcttac ggctggtgaa cgggcagttg
    44221 caggaggaag catgattgca cgttggttct ggcgcgaatg gcgttcgccg tcgctattaa
    44281 ttgtctggct ggcgctaagc ctggcggtgg cctgcgtgct ggcgctgggc aatatcagcg
    44341 atcgcatgga gaagggctta agccagcaaa gccgtgagtt tatggcgggc gatcgggcgt
    44401 tgcgcagttc acgcgaagtg ccgcaagcgt ggctggagga agcgcaaaag cgcggcctga
    44461 aagtcggcaa gcagctgact ttcgccacaa tgacctttgc aggcgacaca ccgcagctgg
    44521 cgaacgtcaa agcggtggat gatatctacc cgatgtatgg cggtctgcaa actaatcccc
    44581 ctggcctgaa accgcaggcg ggcagcgtat tgctggcccc acgcctgatg gcactgctta
    44641 acctgaaaac gggcgacacc attgacgtgg gcgatgccac cttgcggatt gccggagaag
    44701 tgattcagga accggattcc ggttttaacc ccttccagat ggctccgcgt ctgatgatga
    44761 atctggcgga tgtcgataaa accggagccg tgcagccggg gagtcgggtc acctggcgtt
    44821 ataaattcgg cggcaacgag aaccagctcg acggctatga gaaatggttg ttacctcagc
    44881 ttaaacccga acaacgctgg tacggtctgg aacaggacga aggcgcgctg gggcgatcga
    44941 tggaacgctc gcaacagttc ctgctgcttt cggcgcttct gaccttgctg ctggcagtgg
    45001 cagcggtggc ggtagcgatg aatcattact gtcgcagtcg ctacgatctg gtggcgatcc
    45061 tcaaaacgct gggggcaggg cgagcgcaac tgcgtaagct aatcgtcggt cagtggttga
    45121 tggtgctgac gctttcagcc gttaccggtg gggccatagg cctgttgttc gaaaacgtgt
    45181 tgatggtgct gctcaagccc gttctgcctg ctgcactacc gccagccagc ctctggccgt
    45241 ggctgtgggc gcttggcacc atgacggtca tctcgctgct ggtggggcta cgaccatacc
    45301 gcttgttgct ggcaacgcag cctttacgcg tattacgtaa tgatgtggtc gcgaacgtct
    45361 ggccgctgaa gttttatctg ccgattgtca gtgtggtggt tgtgctgctg ctcgccggat
    45421 taatgggtgg cagcatgctg ctttgggcgg tgctggcggg cgcggtagta ctggctttgc
    45481 tgtgcggtgt gctgggctgg atgctgctga atgtacttcg ccgcatgacg ctgaaatcgc
    45541 tgcctctgcg cctggcggtt agccgcctgt tacgtcagcc gtggtcaacg ttaagccagc
    45601 tttcggcatt ttcgctctcc tttatgctgc tggcactgct gctggtgctg cgtggcgatc
    45661 tgctcgaccg ctggcagcag cagctaccgc cagaaagccc gaactacttt ttaattaaca
    45721 tcgccacaga acaggtagca ccgctaaaag cgttcctcgc ggaacatcat atagtcccgg
    45781 aatcatttta tccggtagtg cgggcgcggc tgacggcgat taatgataag ccgacagaag
    45841 gtaatgaaga tgaggcgctt aatcgcgaac tcaatcttac ctggcaaaat acgcggcccg
    45901 atcataatcc gattgtcgcc ggtaactggc cgccaaaagc cgatgaagtg tcgatggaag
    45961 aggggctggc gaaacgctta aacgttgccc tcggtgatac cgtgactttt atgggcgata
    46021 cccaggagtt ccgcgctaaa gtgaccagcc tgcgcaaagt ggactgggaa agtctgcggc
    46081 ctaatttcta ttttattttc cctgaagggg cattagacgg gcaaccgcag agctggctta
    46141 ccagtttccg ctgggagaat ggcaacggca tgttgacgca actcaaccgc cagttcccga
    46201 ccattagcct gttagacatt ggcgcgattt taaaacaggt cggtcaggtg ctggagcagg
    46261 taagtcgggc gctggaggtg atggtggtgc tggtcaccgc ctgcggtatg ttgctgttgc
    46321 tggcacaggt gcaggtggga atgcgccagc gtcatcagga gctggtggta tggcgcacac
    46381 tcggtgcggg gaaaaaactg ctgcgcacga cattgtggtg tgatgttcgc catgcttggg
    46441 tttgtttccg gcctggtggc cgcaattggt gcggaaacgg cactggcagt gttgcaggcg
    46501 aaagtgtttg atttcccgtg ggagccagac tggcgattgt ggattgttct gccgtgcagc
    46561 ggtgcgctgc tgctgtcgct tttcggcggc tggctgggtg cgcgactggt taagggtaag
    46621 gcgctgttca ggcagtttgt ggggtgatga caggtgttag attgcctgat gcgctacgct
    46681 tatcaggcct acgaagagcg tgcaatatat tgattttgca agattgtgta ggccggataa
    46741 ggcgtttacg ccgcatctgg catgaacagt gtgcattttg gcaataaata tgaaaccaac
    46801 tgtcatgtcg gtttttttaa aggccgaagg aggtaaacct tcggccttag ctacgtttcc
    46861 tcagttattc aataaactga cggatcagtt ccagcagcgc gattagcgcc ttaagaacga
    46921 tgagaacttt atcgagaaaa gttttcatcc gttgctccct taagggagaa agacaatcac
    46981 agcttaccct tacgttgcct ataactatga ttttcttcat gtgctagatg aggactgtct
    47041 ctctccaaat tagagcacca gaatcagcac cacctgtatc ctgaccagga cttaaaagta
    47101 cttttccgca ctgcggtaat cccttgaggc agtatcacta ctagcgtcgg gattgtatcg
    47161 ccaacatagc tgtttgaata tgtaggcatc ctgacttata attcacttgc tagatgagga
    47221 ctgtttaatt tctgcccgga accaccaagt cttgggcgga aaaaataaaa aaaccgactg
    47281 tcatgtcggt ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ccagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggaggc gcgttcatcg ttatccatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtggggtgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgcagctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcc
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attaatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ttcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gcaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gagttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt tgaaagttgg aaaatttttc
    51121 caactaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgacgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg gggggttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ctggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc
//
LOCUS       DAJTUP010000016        52348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTUP010000016 DAJTUP010000000
VERSION     DAJTUP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.016"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..105
                     /locus_tag="OO468_000975"
     CDS             <1..105
                     /locus_tag="OO468_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR6258182.1"
                     /translation="GSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(143..1042)
                     /gene="nhaR"
                     /locus_tag="OO468_000976"
     CDS             complement(143..1042)
                     /gene="nhaR"
                     /locus_tag="OO468_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR6258183.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1108..2274)
                     /gene="nhaA"
                     /locus_tag="OO468_000977"
     CDS             complement(1108..2274)
                     /gene="nhaA"
                     /locus_tag="OO468_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR6258184.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            2860..3012
                     /gene="mokC"
                     /locus_tag="OO468_000978"
     CDS             2860..3012
                     /gene="mokC"
                     /locus_tag="OO468_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR6258185.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3116..4246)
                     /gene="dnaJ"
                     /locus_tag="OO468_000979"
     CDS             complement(3116..4246)
                     /gene="dnaJ"
                     /locus_tag="OO468_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR6258186.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4335..6251)
                     /gene="dnaK"
                     /locus_tag="OO468_000980"
     CDS             complement(4335..6251)
                     /gene="dnaK"
                     /locus_tag="OO468_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR6258187.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6628..7032
                     /locus_tag="OO468_000981"
     CDS             6628..7032
                     /locus_tag="OO468_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR6258188.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7058..7771
                     /gene="msyB"
                     /locus_tag="OO468_000982"
     CDS             7058..7771
                     /gene="msyB"
                     /locus_tag="OO468_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR6258189.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            7920..8486
                     /gene="satP"
                     /locus_tag="OO468_000983"
     CDS             7920..8486
                     /gene="satP"
                     /locus_tag="OO468_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR6258190.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8521..9108)
                     /gene="mog"
                     /locus_tag="OO468_000984"
     CDS             complement(8521..9108)
                     /gene="mog"
                     /locus_tag="OO468_000984"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR6258191.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9223..10176)
                     /gene="tal"
                     /locus_tag="OO468_000985"
     CDS             complement(9223..10176)
                     /gene="tal"
                     /locus_tag="OO468_000985"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR6258192.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10455..11885
                     /locus_tag="OO468_000986"
                     /pseudo
     CDS             10455..11885
                     /locus_tag="OO468_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            11955..12731
                     /gene="yaaA"
                     /locus_tag="OO468_000987"
     CDS             11955..12731
                     /gene="yaaA"
                     /locus_tag="OO468_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR6258193.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(12884..13180)
                     /locus_tag="OO468_000988"
     CDS             complement(12884..13180)
                     /locus_tag="OO468_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6258194.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13394..14680)
                     /gene="thrC"
                     /locus_tag="OO468_000989"
     CDS             complement(13394..14680)
                     /gene="thrC"
                     /locus_tag="OO468_000989"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR6258195.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFNLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14681..15613)
                     /gene="thrB"
                     /locus_tag="OO468_000990"
     CDS             complement(14681..15613)
                     /gene="thrB"
                     /locus_tag="OO468_000990"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR6258196.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15615..18077)
                     /gene="thrA"
                     /locus_tag="OO468_000991"
     CDS             complement(15615..18077)
                     /gene="thrA"
                     /locus_tag="OO468_000991"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR6258197.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18103..18222)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18158..18223)
                     /gene="thrL"
                     /locus_tag="OO468_000992"
     CDS             complement(18158..18223)
                     /gene="thrL"
                     /locus_tag="OO468_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR6258198.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18437..19123)
                     /locus_tag="OO468_000993"
     CDS             complement(18437..19123)
                     /locus_tag="OO468_000993"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6258199.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19523..19663)
                     /locus_tag="OO468_000994"
     CDS             complement(19523..19663)
                     /locus_tag="OO468_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258200.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            19759..20475
                     /gene="arcA"
                     /locus_tag="OO468_000995"
     CDS             19759..20475
                     /gene="arcA"
                     /locus_tag="OO468_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR6258201.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20534..21886)
                     /gene="creD"
                     /locus_tag="OO468_000996"
     CDS             complement(20534..21886)
                     /gene="creD"
                     /locus_tag="OO468_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR6258202.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(21944..23368)
                     /gene="creC"
                     /locus_tag="OO468_000997"
     CDS             complement(21944..23368)
                     /gene="creC"
                     /locus_tag="OO468_000997"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR6258203.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23368..24057)
                     /gene="creB"
                     /locus_tag="OO468_000998"
     CDS             complement(23368..24057)
                     /gene="creB"
                     /locus_tag="OO468_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR6258204.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24070..24543)
                     /gene="creA"
                     /locus_tag="OO468_000999"
     CDS             complement(24070..24543)
                     /gene="creA"
                     /locus_tag="OO468_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR6258205.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            24754..25623
                     /gene="robA"
                     /locus_tag="OO468_001000"
     CDS             24754..25623
                     /gene="robA"
                     /locus_tag="OO468_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR6258206.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25620..26267)
                     /gene="gpmB"
                     /locus_tag="OO468_001001"
     CDS             complement(25620..26267)
                     /gene="gpmB"
                     /locus_tag="OO468_001001"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR6258207.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26319..26839
                     /gene="yjjX"
                     /locus_tag="OO468_001002"
                     /pseudo
     CDS             26319..26839
                     /gene="yjjX"
                     /locus_tag="OO468_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(26924..27250)
                     /gene="trpR"
                     /locus_tag="OO468_001003"
     CDS             complement(26924..27250)
                     /gene="trpR"
                     /locus_tag="OO468_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR6258208.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27340..29277)
                     /gene="sltY"
                     /locus_tag="OO468_001004"
     CDS             complement(27340..29277)
                     /gene="sltY"
                     /locus_tag="OO468_001004"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR6258209.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29488..31155
                     /gene="ettA"
                     /locus_tag="OO468_001005"
     CDS             29488..31155
                     /gene="ettA"
                     /locus_tag="OO468_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR6258210.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31262..31429)
                     /locus_tag="OO468_001006"
     CDS             complement(31262..31429)
                     /locus_tag="OO468_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258211.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31463..32695)
                     /gene="nadR"
                     /locus_tag="OO468_001007"
     CDS             complement(31463..32695)
                     /gene="nadR"
                     /locus_tag="OO468_001007"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR6258212.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32716..34098)
                     /gene="radA"
                     /locus_tag="OO468_001008"
     CDS             complement(32716..34098)
                     /gene="radA"
                     /locus_tag="OO468_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR6258213.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34147..35115)
                     /gene="serB"
                     /locus_tag="OO468_001009"
     CDS             complement(34147..35115)
                     /gene="serB"
                     /locus_tag="OO468_001009"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR6258214.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35221..35865
                     /locus_tag="OO468_001010"
     CDS             35221..35865
                     /locus_tag="OO468_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR6258215.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            35893..36908
                     /gene="lplA"
                     /locus_tag="OO468_001011"
                     /pseudo
     CDS             35893..36908
                     /gene="lplA"
                     /locus_tag="OO468_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            36940..37203
                     /locus_tag="OO468_001012"
     CDS             36940..37203
                     /locus_tag="OO468_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6258216.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37364..38083)
                     /gene="deoD"
                     /locus_tag="OO468_001013"
     CDS             complement(37364..38083)
                     /gene="deoD"
                     /locus_tag="OO468_001013"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR6258217.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38140..39363)
                     /gene="deoB"
                     /locus_tag="OO468_001014"
     CDS             complement(38140..39363)
                     /gene="deoB"
                     /locus_tag="OO468_001014"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR6258218.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39415..40737)
                     /gene="deoA"
                     /locus_tag="OO468_001015"
     CDS             complement(39415..40737)
                     /gene="deoA"
                     /locus_tag="OO468_001015"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR6258219.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(40864..41643)
                     /gene="deoC"
                     /locus_tag="OO468_001016"
     CDS             complement(40864..41643)
                     /gene="deoC"
                     /locus_tag="OO468_001016"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR6258220.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            41901..43442
                     /locus_tag="OO468_001017"
     CDS             41901..43442
                     /locus_tag="OO468_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR6258221.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43414..44277
                     /locus_tag="OO468_001018"
     CDS             43414..44277
                     /locus_tag="OO468_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR6258222.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44491..45273)
                     /locus_tag="OO468_001019"
     CDS             complement(44491..45273)
                     /locus_tag="OO468_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6258223.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45270..46343)
                     /locus_tag="OO468_001020"
     CDS             complement(45270..46343)
                     /locus_tag="OO468_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR6258224.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46465..46626)
                     /locus_tag="OO468_001021"
     CDS             complement(46465..46626)
                     /locus_tag="OO468_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR6258225.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46743..47348)
                     /gene="osmY"
                     /locus_tag="OO468_001022"
     CDS             complement(46743..47348)
                     /gene="osmY"
                     /locus_tag="OO468_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR6258226.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47741..49330)
                     /gene="prfC"
                     /locus_tag="OO468_001023"
     CDS             complement(47741..49330)
                     /gene="prfC"
                     /locus_tag="OO468_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR6258227.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49421..50098)
                     /gene="yjjG"
                     /locus_tag="OO468_001024"
     CDS             complement(49421..50098)
                     /gene="yjjG"
                     /locus_tag="OO468_001024"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR6258228.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50113..50559)
                     /gene="rimI"
                     /locus_tag="OO468_001025"
     CDS             complement(50113..50559)
                     /gene="rimI"
                     /locus_tag="OO468_001025"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR6258229.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50528..50941)
                     /gene="holD"
                     /locus_tag="OO468_001026"
     CDS             complement(50528..50941)
                     /gene="holD"
                     /locus_tag="OO468_001026"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR6258230.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51044..52075
                     /gene="rsmC"
                     /locus_tag="OO468_001027"
     CDS             51044..52075
                     /gene="rsmC"
                     /locus_tag="OO468_001027"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR6258231.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12443 a        14188 c        13126 g        12591 t
ORIGIN      
        1 ggaagtaatc agattcctct atttattcgc ccgcgtacat atggaaacaa tgtctcaagc
       61 gggcaaataa tgagccgagt caaaatcgtg gtaatgtaca actaatctat ctcccccacc
      121 ttaactttgg gggagctgcc gattaacgca ccgctggact aaaaagcgca gaataatccg
      181 tattgcagat tcgctgtacc gccgggtgct gaatcatccg ctcagcaaaa atagcatggt
      241 actcttccat cacattctcg acgcgaccaa tttctacgac agttttatcg gcataaaagt
      301 catatgcata aagcgttggg gcaacgaaga ttgcattgtg catcgcacca aaagctttca
      361 tcaaagcggc atcatcaaac tcgccgagga tttctacgtt taatccctgg gagttaaacc
      421 agttaagcaa tttgcgccct aacattgaac gtcgcccagg aatcaaaagt cgccgttctt
      481 ccagacaagc cgggaacggt ttttctggtg gtggatttgt acaccagaaa ctcacgccac
      541 attcgccaat tctcacggag aacaggcctt cctgctgcgt agagtctatc ggacagtcag
      601 aaatgatcat atccagttta tgctgactta attgctccag cagcatttcg tgggtggatt
      661 cgaagcagcg aagatgaatg ggctcgcctt ctaccactgc ggcgttaagt acgctactga
      721 ccaggcgttt ggaaagtgca tcagccacgc caacgtcaaa caataaattg gattctttgc
      781 gatagttcac aatatccagc atttcctggc ttaaggtgaa cattttatcg gcatagcgat
      841 agaccagttc tcccagctcg ctgggttcga gaccacgtcc cttgcgttta aataatttgc
      901 cttgcaggcg ctcttccagc gctcgaatct gtccggtaat ggtttgtgga gttaaataaa
      961 gcgcctccgc tgcgccaacc acggaacctt ctttatagac atgccagaag taatacaagt
     1021 ggttgtaatt gatatgagac atgctcattt ctctccctga taacaatgaa aagggagccg
     1081 tttatggctc cccggtaaac cgtcctgtca aactgatgga cgcaaacgaa cgcgtaacca
     1141 gctgtatcca attaccgccg aagagataga accgaccagg ataccgagtt tcgcccagtt
     1201 aatcagttct ggatctacgc taccaaaggc caggctggca ataaagatag acatagtaaa
     1261 accgataccg cacaggatcc ccaccaccat aatttgctga taagtcgttc cctcaggcag
     1321 atgcgccagt ttcaaacgca gcgccaacca gcagaacaga ctaatcccca gcggtttgcc
     1381 aatcagcaag ccagcgatga tccccaatgg cagaatggag gtcaagccat ccagcgtgac
     1441 gccttgcagt gaaacgccag cattagcaaa tgcaaacagc ggcaaaatca gatacgccac
     1501 ccacgggtgc aacacatgct ccagtcgctt cgctggagaa cgcccatgct tctctttcaa
     1561 aggaataaag aagccgacaa ttacccccgc cagagttgcg tgaacccccg atttcaacac
     1621 cgcagtccac aacaccacgc caacaagaat atagacgccc gtgcggcgta caccacacag
     1681 attcaatacc gcgagtaccg caattgctac agccgcgacg ccaagggagg ccatcgataa
     1741 gtcattagtg tagaacaatg cgatgataat gatggcccca agatcgtcga taatagccag
     1801 agccatcaaa aagatcttca gcactaacgg aacacgactt cccaacagcg ccagtacacc
     1861 aagtgcaaaa gcaatgtcag tagccgccgg gatcgcccac ccttcgcggg taatcggatc
     1921 ggcatagtta aaagccagat agagtaatgc cggcacaatc atcccaccaa tagcggcgat
     1981 aactggaaat gcggcctggc gtaagctggc tagcgatcct tgcatcagtt cacgtttaac
     2041 ttccagaccg actaacagga aaaataccgc catcagcgca tcatttatcc ataacagcat
     2101 gtttttgttg atttcgagtg aaccaacccg gagctgaacc ggcgtctcca gaaagtcgtg
     2161 ataccatcca ctggttgcgc cgctgttggc cataatcatc gccaggatag cggcaatgat
     2221 aagaataatg cctcccgagg catcactgct aaagaatcga tgcagatgtt tcacttttta
     2281 tttctctttc aggtgaatag atcgttttta ctattttacc ccgcacgaat catcgttaaa
     2341 atcagattat agtgtaggaa taattcgttt tttacgagcc attgaagaat ttgcgccatc
     2401 agcggtgaga tgcgtttttg acggctgtaa gcgctcacga gtgaccctaa gcccagataa
     2461 tcatagctta aggtgaggat tcctgaggga ttaattagga aaagtaataa ttaatttgtt
     2521 gtcagtcggc gatgcttgtt aggccaatat ttcaaaaata atccagcttg tcgtcaataa
     2581 ttgcgatgaa gagataaatt aaagttaaat aattaaatca ttaaaaaatt acgccacaaa
     2641 aatgcgaggc gtcttcagga ttagaatcga taaaacaaaa tgggttaaag agagcgatat
     2701 aatagcggcg ggtgcttgag gctgtctgtc tcaggcatta gctgaacggc agatagagaa
     2761 aagccccgag tgatatttta ccatcaaccc gaggcctcct atatgctgaa cacatgtaga
     2821 gtgcctctta ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca taaggcgatg
     2881 attgtcgccc tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt aacgagaaaa
     2941 gacctctgtg aggttcacat ccgaactggc cagacggagg ttgctgtttt cacggcttac
     3001 gaatccgagt aagagcaacg gcggggagtg atccccgcca ctttacaggt gctcgcatat
     3061 cttcaacgca ccctattttt acccaggcct gcccacgggc aggcttttgg ggaggttagc
     3121 gggtcaggtc gtcaaaaaac ttcttcacac catcaaagaa gctctttgag cgcgggctgt
     3181 tgtgctcgcc ggttgggcca ccgaagcttt cttgcagctc ttgcagcagc tgtttctgct
     3241 tttcgttcag gcctaccggt gtttcgacga caacgcggca cagcaaatca ccctgtgcgc
     3301 caccgcggac agacttgacg cctttaccgc gcatacggaa tagcttaccg gtctgggttt
     3361 cgccaggcac tttcagtttg acgcgaccat caagggtcgg tacttcgatt tcgccaccca
     3421 gcgccgccat agcgaagttg atcgggactt cgcaatacag gttgttgcct tcacgctcga
     3481 aaatcgggtg ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt gcgccatgct
     3541 cgcccgcttc accttcgccc gcaagacgga tgcggtctcc agtgtccacc cctgccggga
     3601 ttttaacgga cagcgttttg ctgcgctcaa cacgaccatg accatgacat ttgttgcacg
     3661 gatctttgat cagcgtaccg cggccctgac agtgtggaca ggtctgctgt acagcgaaga
     3721 atccctggcg catctgcacc tgaccagaac catgacaggt cggacaggtc tgcggctgtg
     3781 tacctggttt tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc ggaatgcgga
     3841 tctctttggt cacgccacgt acagcttctt cgagggtgag ctccatgtta tagcgtaaat
     3901 cagcaccgcg cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg ccgaaaacgt
     3961 caccaaaaat atcgctgaag tctgcgccgc cgccaaaacc accgccgccc atgcctcctt
     4021 gctcaaacgc agcatgacca tactgatcgt atgccgcacg tttttgcgag tcggtcagaa
     4081 cttcataagc ttccttgatc tctttaaatt tcgcctcggc ctctttgtca ccctggttac
     4141 ggtccgggtg gtatttcatg gccaggcgtt tgtaggcctt tctgatttca cgctcttccg
     4201 ctgttttgga aacgcctaaa atctcgtaat aatcttgctt agccatcttt tttaaattgc
     4261 ccctagatga atgcacgggc ggagaggaaa ttccccttcg cccgtgtcag tataattacc
     4321 cgtttatagg gcgattattt tttgtctttg acttcttcaa attcagcgtc gacaacatcg
     4381 tcatctttcg cgttgtttgc agaagcatca gcaccggcag tctgctgctg ggcatgttgc
     4441 tgctgggcga tttccatcag tttctgggaa acctgtgcca gttcctgcat tttcgcttcg
     4501 atagcggctt tgtcttcacc tttcagagca gtttccagtg cagtcagcgc agactcgata
     4561 gcagttttgt cgtcagccgg cagtttgtcg cctgcttctt caacctgctt acgggtgctg
     4621 tgcagcagat ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt acggtcagct
     4681 tcggcgttag cttctgcgtc gcgtaccatt ttctggattt catcttcgtt cagaccagaa
     4741 gaagccttga tggtgatctt ctgctcttta ccgctgtttt tatctttcgc ggaaacgtgc
     4801 aggataccgt cagcatcgat atcgaaggta acttcgatct gcggcatgcc gcgcggtgcc
     4861 gggttgatac catctaggtt gaactgaccc agagatttgt tatcagccgc acgtttacgt
     4921 tcaccctgca gcacatggat ggttaccgca gactggttgt cttcagcggt agagaacacc
     4981 tggctgtgct tggtcgggat agtggtgttt ttcgcgatca gcgtcgtcat cacaccgccc
     5041 atggtttcga tacccagaga cagcggggta acgtccagca gcagtacgtc ttttacgtca
     5101 ccagtcagaa caccaccctg aacagcagca ccgattgcta cagcttcgtc cgggttaacg
     5161 tctttacgcg gctctttacc aaagaactca gcaactttct tctgaaccat tggcatacga
     5221 gtctgaccac caacgaggat aacgtcgtcg atatcagata cggacaggcc agcatcctgc
     5281 agtgcaactt tcagcggctc aatggaacgg tttaccagat cttcaaccag gctttccagt
     5341 ttcgcacgag tcactttgat gttcatgtgt ttcggaccgg tcgcgtctgc agtgatgtat
     5401 ggcaggttaa cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt ttctgccgct
     5461 tctttcaggc gctgcattgc cagcggatcg ttgcgcaggt caatgccctg atctttcttg
     5521 aattcttcaa ccagatagtt gatcagacgg ctgtcgaagt cttcaccacc caggtgggta
     5581 tcaccgttgg ttgccagaac ttcgaaggtt ttttcgccgt caacttcgtc gatttcgata
     5641 atagaaatat cgaaagtacc accccccagg tcataaaccg cgatagtacg gttgccagtg
     5701 cctttgtcca gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat acgttttact
     5761 tccagaccag cgatacggcc tgcgtctttg gttgcctgac gctgagcatc gttaaagtat
     5821 gccggtacgg tgataacagc ttcagttacc ggttcaccca ggtaatcttc agcggttttc
     5881 ttcatttttt tcagcacttc agcagaaatc tgcggcggtg ccattttctg gcctttaact
     5941 tcgacccatg cgtcgccgtt atcagcagca ataattttga acggcatgat ggaaacatca
     6001 cgctgtactt cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc aaacagggtg
     6061 ttttgcgggt tcgtcactgc ctgacgttta gccggctgac caaccagagt ttcaccatcc
     6121 tgggtatagg caatgataga aggcgtggtg cgatcgcctt cggcgttctc cagcacgcga
     6181 ggaatggtgc catccataat cgctacacaa gagttggtag tacccaggtc gataccaatt
     6241 attttaccca tctaaacgtc tccactatat attcggtcat catgtggttg tgagtctgta
     6301 ataggggcga aacgtctggt ttcaactgcc caatttcatt tttttgcaga ctcactcact
     6361 gcggttgact actaaatggg gtcgtaaccc acgtcatcaa gggggagatg caaaaatttt
     6421 ttgtgctttt gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat tgacagcaat
     6481 tttattcata taaagaatga atcgcagcca aggcgttcat caaaaaattg taataaaaag
     6541 aaaagattac gtgcctgaat cttctcttta tcagcagtaa actagtgggt attcatcccc
     6601 ctacctcttc ccactaagag aatccttatg aaatccgttt ttacgatttc cgccagcctg
     6661 gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg accataaact cctcggcgtc
     6721 attgcaatgc cgcgtaacga aaccaacgat ctggcgctga aacttcctgt ttgtcgcatt
     6781 gtgaaacgca tacaactctc tgccgaccat ggcgatttac agttaagtgg tgcatcggtt
     6841 tatttcaaag ccgcccgtag cgccagtcag agcctgaata ttccttcaga aataaaagaa
     6901 gggcaaacca ctgactggat caacattaac agcgataacg acaataaacg ctgcgtctca
     6961 aaaatcacct tttccggtca tacggtgaac tcatcggata tggccacgct gaaaattatc
     7021 ggcgacgact aacgccagaa tatagaagcc acaaaaaatg aatgttaatt acctgaatga
     7081 ttcagatctg gattttcttc agcattgtag tgaggaacag ttggcaaatt tcgcccgatt
     7141 gctcacccat aatgaaaaag gcaaaactcg cctctccagc atactgatgc gcaacgaact
     7201 gtttaaatcg atggaagggc atcccgagca acatcgccgc aactggcagc tgattgccgg
     7261 agaattacag cattttggtg gcgatagtat cgccaacaaa ctgcgcggac acggtaaatt
     7321 gtatcgggcc attttgctcg atgtttcaaa gcgattgaag ctgaaagccg acaaagagat
     7381 gtctacgttt gaaattgagc aacagttact ggaacaattt ctgcgtaata cctggaagaa
     7441 aatggacgag gaacataagc aggagtttct gcacgcggtc gatgccaggg tgaatgagct
     7501 ggaagagctg ctgccgctgc tgatgaaaga caaattattg gcaaaaggtg tgtcgcattt
     7561 gctttccagc caactgaccc gcattttacg cacccacgca gcaatgagcg tacttgggca
     7621 tggtttgctg cgcggcgcgg ggctgggagg ccctgtgggc gcagcattaa atggggttaa
     7681 agcggtcagc ggcagcacct atcgcgtgac gattccagcc gtactgcaaa tcgcctgcct
     7741 gcgccggatg gttagcgcca ctcaggtctg ataccggaaa ccggggaaaa ttttcatttt
     7801 cccctcaaaa gatcgtagac actgccccac tggctgatta ttatgccgcg ccctgaaaac
     7861 actacagtta ttcagggaaa ttatttcacc attcattcga tgatgatttt tgaggaatta
     7921 tgggcaacac taagttggct aatccggcac cgctgggcct gatgggcttc ggcatgacca
     7981 ccattctgct taacctgcac aacgtgggtt atttcgctct ggacggtatt attcttgcca
     8041 tgggcatttt ctacggcggc atcgcgcaaa tttttgctgg tctgctggag tacaaaaaag
     8101 gcaacacttt cggtttaacc gcattcacct cttacggttc tttctggctg acgctggttg
     8161 cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag ttccttggtg
     8221 tctacctggg tctgtggggc gtatttacgc tgtttatgtt cttcggcact ctgaaaggcg
     8281 cgcgcgtcct gcaattcgtt ttctttagcc tgaccgtgct gtttgccctg ctggcgatcg
     8341 gtaacattgc cggtaatgcc gcgatcatcc actttgccgg ctggattggt ctgatctgcg
     8401 gtgccagcgc aatctatctg gcgatgggtg aagtgctgaa cgagcagttt ggtcgcaccg
     8461 ttctgccgat tggtgaatcc cactaattgt cttgagatcc ccccgctcgg ggggattttt
     8521 ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca cttctggtgc
     8581 cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg caaaaatacc
     8641 atgcaccaca acgttaccct cagcgtcctt cacaccttcc agcgtctctt taatagactt
     8701 cggctgaccg ggtaagttaa ggatcagcgc ctgtttgcga atcacgccca cctgacgcga
     8761 aaggatcgca gttggtacaa aatgcaggct gatctggcgc atctgttcac caaagccagg
     8821 catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac gcgccgggcc
     8881 agttccgccc gtggtgagca ccagatggca actcatttca tccaccagct cacacaacgt
     8941 ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa acggcgtggt
     9001 tagcgccgat gtcagccatt cttccagcgc tggaatgcct ttatcctgat aaacgccgct
     9061 ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca agatttttcc
     9121 gtttaaacaa tctcgttaaa cagaatgata cactgcgaag ggagtgacag acaggcttca
     9181 gaagaggtag cgtgaccgac ttcccggtca cgctaagaat gattacagca gatcgccgat
     9241 cattttttcc agtttttcct ggtcaatagc aaacttacgg ataccttccg ccagtttatc
     9301 tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga tacgcgccgg
     9361 acgcgctttc acttcgccag tgtaagacag tttacgttcg atagcccctt cgctctccgc
     9421 cagctctttc agcagtgccg gtgcgatggt cagacggtca cagcctgcca gttccagaat
     9481 ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccgt gctctttgta
     9541 gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag cgtactcttt
     9601 cttatcggta ttcgctttgt accagtcaag aatacggcca acaaacggcg agatcaggaa
     9661 cacgcccgcc tccgcacaag cacgagcctg agcgaaggag aacagcaggg tcaggttaca
     9721 gttgatacct tctttttcca gctgttctgc agcacggata ccctgccagg tagaagccag
     9781 tttgatcaga atacgatcgt tgctaatacc tgcatcgttg tagagtttga tcaggcgttt
     9841 tgctttcgca attgacgctt cagtgtcata ggaaagacgc gcatcaactt cagttgagat
     9901 acggcccgga accagtttca ggatttccag accaatattt actgccagtt tgtcggtcgc
     9961 gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga cagcatcatc
    10021 aatcaactta cggtattccg gaatctgcgc tgcgttaaga atgagagaag ggttggttgt
    10081 ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg ccactacggt
    10141 ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct ttaaacagct
    10201 tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc agcgcaaccg
    10261 ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg cgttgatatc
    10321 gcccaccatt tttatacaaa acctcatgta tgctacgcag aagttatcaa gtacctcgta
    10381 gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg cgaccggcaa
    10441 taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt tggggatcgg
    10501 taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc ggatttgtgc
    10561 agtttcgcta catccgccag tttggcaaaa gtcttaaaaa tagcattcat ccacagccag
    10621 gcggtttaac ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg ggtagcggca
    10681 acctggccgg cgttgcgctg gcaattaccg ccggtggacc aggtgccgtc ttttggatgt
    10741 gggttgctgc gtttatcggc atggcgacct cgtttgccga atgttccctt gcacaacttt
    10801 ataaagaacg tgacgtcaat gggcagtttc gtggcggacc ggcatggtat atggcgcgcg
    10861 ggctggggat gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc gcctacggca
    10921 taattttcag cggagttcag gcgaacgccg ttgcccgagc cctgagtttt tcttttgatt
    10981 ttcctccgct ggtgacgggc attattctcg ctgtctttgc tctgctggca atcactcgtg
    11041 gtcttcatgg cgtcgcccgg ctcatgcagg ggtttgtccc gttgatggcg ataatctggg
    11101 tactgaccag cctggtgatt tgcgtaataa atatcgggca acttccccac gtcatttggt
    11161 ctatttttga gagtgctttt ggctggcagg aagcggcagg cggcgcggcg ggatatacct
    11221 taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag gtgggaatgg
    11281 gatcgacgcc aaacgcggca gcggcagctg catcctggcc tccgcatccg gcagcacaag
    11341 ggattgtcca gatgattggc atttttatcg acaccctggt catctgtacg gcaagcgcca
    11401 tgctgatatt actggcgggt aacggcacaa cctacatgcc gctggaaggt attcagctta
    11461 tccagaaggc gatgcgggtg ctaatgggtt cctagggtgc tgaatttgtt accctcgtgg
    11521 ttattctgtt tgccttcagc tccatcgttg ccaactacat ttatgccgaa aacaatctct
    11581 tctttttacg cctgaacaac cctaaagcga tctggtgttt gcggatctgc accttcgcaa
    11641 cggtcatcgg cggcaccttg ctaagccttc cgctgatgtg gcaactggca gatatcataa
    11701 tggcctgcat ggctattacc aatttgaccg ccattttact gctctcgcct gtggttcata
    11761 ccattgccag tgattatcta cgccagcgta aactcggcgt gcgcccggtg tttgatccgt
    11821 tgcgttatcc ggatatcggt cgccagcttt ctccggacgc gtgggatgat gtttcgcagg
    11881 agtaatcaca actatcgatc aactcattct ctttttttgc taaagtcggc ataaatttcc
    11941 tgcaaggact ggatatgctg attcttattt cacctgcgaa aacgcttgat taccaaagcc
    12001 cgttgaccac cacgcgctat acgctgccgg agctgttaga caattcccag cagttgatcc
    12061 atgaagcgcg gaaactgacg cctccgcaga ttagcacgct gatgcgcatc agcgacaaac
    12121 tggcgggtat caacgccgct cgctttcatg actggcagcc agatttcacg ccggcgaatg
    12181 cccgccaggc gattctggcg tttaaaggtg atgtctacac cggcttgcag gccgaaacct
    12241 tcagcgaaga cgatttcgat tttgcccaac agcatttgcg aatgctttcc ggcttgtatg
    12301 gcgtactgcg cccgctcgat ttaatgcagc cttatcgtct ggaaatgggg atccgtcttg
    12361 agaatgcccg agggaaagat ctgtatcaat tctggggaga tatcatcacc aacaagctga
    12421 acgaggcgct cgcagcacaa ggcgataatg tggtgattaa tctggcgtca gatgagtatt
    12481 ttaaatcggt gaagccgaag aaattgaatg ccgagattat caagccggtg ttcctcgatg
    12541 agaagaacgg caaatttaag atcatcagct tctacgctaa gaaagcacgc ggtctgatga
    12601 gtcgtttcat tattgaaaat cggctgacca aaccagagca actgactggt tttaatagcg
    12661 aaggttactt ctttgatgaa gattcctcca gcaatggcga actggtgttt aaacgctacg
    12721 agcagcgtta agtttgtagt caatgccgga tgcggcgtga acgccttatc cggcctacaa
    12781 aagcatgcag attcaatata ttgcagaatt aacgtaggcc tgataagacg cgccagcgtc
    12841 gcatcaggcg ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc
    12901 ctggaccatg accgccgtga tgatcatgag gagctttctt atggtggcgc ggcggtggcg
    12961 gcggtccgtg tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt
    13021 ggtcgcgcca gtgacctcca tcccagtaat agccacgatt atcacgatcg cctatctgta
    13081 attttactga cgggactaac gtaatttccg cagcctgtgc tgccatggga gcgaccagaa
    13141 ccagggaaag tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt
    13201 gcccgttatg ggtcgatatc cggagattac gcgatgagaa cgacggttgt tattctccgc
    13261 aatcctaatc tctaagttac cgcatttatt gataatttct cctttttccc tgtcgttttt
    13321 ctccataatt tcttcataaa aaagccgggc tgcataaaag caaacccggc ctgattgaga
    13381 taatgaatag attttactga tgattcatca tcaatttacg caacgcagaa aaatcggcgg
    13441 gcagattatg tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca
    13501 acgtttcacc gagaatcgct tccacgctct ctttaaattt cgccggatgc gcggtgccga
    13561 ggaacaagcc atattcgcct ggattcaact ggtcacgcag cgcacgatac gctacggcag
    13621 cgtgcggctc cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt
    13681 catcatccac ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact
    13741 cttccacacg cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct
    13801 gagtcgcttt gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt
    13861 tggtcgcagc aataaaacgt ttcaccggca gaccgaatga cttcgccagc agacccgccg
    13921 tcaaatcgcc gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcctcctgcg
    13981 gcagctgcgc aacagcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa
    14041 tcgagttagc cgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct
    14101 tcaccagcgc ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc
    14161 ccaacgtaca gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa
    14221 ccactttcac attcggcaaa ccgtagaaag catgagccac tgcagctccg gtatcaccgg
    14281 aggtcgcggt cagaatggtc actggcttat catccgcaat atgggtcagc atttgtgcca
    14341 taaagcgacc gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac
    14401 cgacatcgct ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct
    14461 cttccaggat ttcctgtggg atttcatcac caataaacgc cgagaggatc ttcgcactgc
    14521 gggtgacaaa atccagcttc agcatctcat caatttcagt caggttgaat tccggcaggt
    14581 cgtgcggaaa aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc
    14641 tgacctgctc gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg
    14701 cgcccgccgt atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct
    14761 tacccaacca gtcggcaacg cgctgggcgg tatccggctt gtcacataga gcgaacaagg
    14821 tcgggccgga gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg
    14881 cctgccggaa gccaggcagt aaccgttcac ggtagggttc agcgataaca tctttcatca
    14941 gcttcgcggc aagctcaggc tgacgggaat agcaggcgtg aatgaagcca gccagatgtc
    15001 gcccgtgcgc aatgcaatcc tggcggcgat actgcgccgg taaaatagcc cgggcttctg
    15061 ccgtcgagac tttaattccc ggatacgcca gcacccacag ccactcatca aaccctggca
    15121 cttgctggct gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg
    15181 gtgccacgtt gtcgtaatga atgctgccgg agatacgtcc ttccagctcg cccatcaaag
    15241 ccagcaaacg agtgtcatta agcggcttgc cgcagtgttc attcatcgcc atcagcgccg
    15301 cgaccaccga acaggcgctg gagcctaagc ccgaaccgat cggcatattc ttttccagag
    15361 tcatcgccac tggaatttgc ttgccaagct cctggcaaaa acgctcccag cactgataaa
    15421 cgatattttc ccgtggttct gacggcagct tatcggcaaa gcgtccgagt ttgttgagac
    15481 tgaatgtctc tgccgcctca accgtgacta catctccgag caatgcacca tcaacgggtg
    15541 ccaccgccgc cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat
    15601 aaactttaac catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag
    15661 acaccggcag cggtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc
    15721 tgataatagt ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga
    15781 tcattcccat ccacttcggc aatcttcacg cggcagacac catcttcatc aatattgcca
    15841 acatagcgca aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg
    15901 tcgagctgtg acagattcgc cataaaagcg gcaacatcac cctcggcgtt aaactctgcg
    15961 ggcagcacag gttcaatttc aatatccgcc agctccagtt cacgtcccgt ttcacgagcg
    16021 agaatcaata gtttacgcgc cacatccata ccagaaagat catctcgcgg gtccggttcg
    16081 gtataaccca tttcccgcgc cagcgtggtc gcctcggaga aactcatgcc ttcgtctaac
    16141 ttgccgaaga tataagaaag cgaacctgaa agaatgccgg agaacttcac caattcatca
    16201 cctgcattga gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg
    16261 tcatagagga atttacgccg cgatttttcc gccgcataac gcaactgatg atagtaatcc
    16321 atcgacgagg tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaggaag
    16381 tcggcatatt gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc
    16441 agatgatatt ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgt
    16501 gcaagttctt cctgccagtt ttccagatta aggccatgca cactagtgag cagagccttc
    16561 gagttggcaa cgccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgctga
    16621 cgcttcagtt gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg
    16681 ataacctgat cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca
    16741 tcgttattta ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg
    16801 ttgatattgg cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc
    16861 ataccatcac ctaccaccga gataatggcc agccgttccg tcaccgccag cggctccagt
    16921 aagccttctt tcagttccag gtagaactct tcctgcattg cccgttcagc tcgcacacag
    16981 tcgctttgtg gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc
    17041 acggaaatac gggcgcgtga cattgcagca aagacgcgcg ccgccatgcc aaccatccct
    17101 ttcatccccg ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg
    17161 accggtaatt cgtcttcatc acggctggca ccaatgagcg tacctggtgc ttgaggattt
    17221 ccggtatttt taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg
    17281 tgaagaactt tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc
    17341 aacaacctcg catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc
    17401 caaatctcgc aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa
    17461 ccgttacgtc caagcaccac cagttcgcct ttttcattac cggcggtgaa acctgccatc
    17521 aacaccatgt gatcagccgg aatgcggctt gccgcaatac ggcgggtgga ctcagcaata
    17581 tcgacggtag attcgaggta atgccccact gccagcagtt tttcgaccgg atcgataacg
    17641 gtaacgttgt gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg
    17701 ccacggcaaa tcagcgcagc gttgatgcta tccgggcact gccccaacaa actaatgcca
    17761 tgcaggacat gttttatttg ggcaaattcc tgatcgacga aagttttcaa ttgcgccagc
    17821 gggaaccccg gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca
    17881 tcgctgatat tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg
    17941 tggttggtga ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg
    18001 ctttccagaa tatcggcaac acgcagaaaa cgttctgcat ttgccactga tgtaccgccg
    18061 aacttcaaca ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa agcccgcact
    18121 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
    18181 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
    18241 taatttttat ctgtttgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
    18301 aataaaattt taatttactc acggcaggta accagttcag aagctgctat cagacactct
    18361 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
    18421 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
    18481 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
    18541 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
    18601 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
    18661 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
    18721 tcggcacacc agtaagcacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
    18781 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
    18841 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actacgcgca gtggtggcga
    18901 cagtgaaatc gacatcgtgt aacgattcag ccagtgtcgg gaaaacttta atattatcaa
    18961 taatatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
    19021 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
    19081 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
    19141 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
    19201 tcttaacgta agccgcagaa aaagcccttt acttagctta aaaaaggcta aactattttc
    19261 tgactgcact aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
    19321 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
    19381 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
    19441 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
    19501 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
    19561 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
    19621 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
    19681 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
    19741 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
    19801 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
    19861 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
    19921 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
    19981 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
    20041 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
    20101 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
    20161 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
    20221 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
    20281 aacccaggca aaattcagtc tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
    20341 aaaccgcacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
    20401 acgccggata cgccggaaat catcgccacc attcacggtg aaggttatcg cttctgcggt
    20461 gatctggaag attaatcggc tttaccaccg tcaaaaaaac ggcgcttttt agcgccgttt
    20521 ttatttttca accttatttc cagatacgta attcatcgtc cgttgtaact tctttactgg
    20581 ctttcatttt cggcagtgaa aacgcatacc agtctatatt acgagtcaca aacatcatgc
    20641 cggccagcgc caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga
    20701 gcagtcccca catcactcca tccagcaaca acagcgcgag ggtaaacaac atgctattgc
    20761 gccagccttt caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg
    20821 cgattatcca tgccacggta aaaccgatat gttcagaaag cgccagcaag agcaaataaa
    20881 acatcaccaa tgaaagcccc accagcaaat attgcattgg gtgtaaacgt tgcgcggtga
    20941 gcgtttcaaa aacaaagaac gccataaaag tcagtgcaat cagcagaatg gcgtacttag
    21001 tcgcccggtc cgttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg
    21061 ggaagttttc ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttattagcaa
    21121 accagctgct ttgccattgc gcctgaaaac ctgactcgct agcttcccgt ttggctggta
    21181 gaaaatcacc taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac
    21241 gcccggcagg caccacagaa agatcgccgg taccgcttaa attcagtgcc atattcagct
    21301 gcaggttctg cttccgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt
    21361 gctctaaccc ggtaccgggt tcaatggtca gtgccgttcc gttaacttca ggcgctttca
    21421 ccacaccaat accacgcgca tccccgacgc taatcacaat aaagggcttg cctaagatga
    21481 tatttggcgc gttgagctcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat
    21541 cactgtgcca gacctgaccg gtataaatcc ctatcttgcg ttcttccacg ttctgattgc
    21601 catcaaccat caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg
    21661 ttttatcctc ttcctgcacc gtataaagct cggtcacagg aatagcgata agcggcccaa
    21721 cgagtttttg cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat
    21781 cggcacgttc gacaatcact tgccgaatca gcattatcgg aatcaacaac agcaatactg
    21841 caccaaacaa ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg
    21901 caatggcagc aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt
    21961 gaagtcgtag cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt
    22021 taaacaaacg ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat
    22081 ttgcacgcgg caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac
    22141 cactaccggt atccagcacc ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg
    22201 ttatgcaacc gctctcggga gtaaaatcga tggcgttatc cagtaaattc cccagcgcct
    22261 gatccagtaa cgccggttca gcagcaacat taacctcggt gggcataaca tgcaaagtga
    22321 tgtttttttc tgccaactgc acggtgcgcg cttcgctgac gcggcgaaat aatgccgcca
    22381 catcaacagc agtcagaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg
    22441 tttccaccag cgcctgcatt cgcgcatttt gtgtcagaat gttgtcagta aaacgagcca
    22501 ctacttcagg cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg
    22561 ggctttttag ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca
    22621 gcttcacgcg catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat
    22681 cgggaagagg aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa
    22741 tggagcggtt gatccaccaa accatgcctg cgccaatcac cagtgcaatc cccaacaaaa
    22801 tggcgctggc ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt
    22861 tcggtttgcc tacgctcaaa acgccaataa gccgcgagcc gcccataatc ggcgcggcaa
    22921 catacatcac cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact
    22981 gaccacgcaa cgttagccag acgtcattcc agcgcgaata atcctgtcca acggctttat
    23041 ttgccgaatc gaacaatact ttgccatgcg catcggtcat atagacatga tattcattgc
    23101 gcactttgtt aatgccacca atattggcgc gaaacgggcg atgttgtagc tgattaaacg
    23161 cctgcgccag ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg
    23221 caagtaacgt tgccgtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa
    23281 cttctttgac aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc
    23341 ccagcagcaa ccgcatgccg atacgcatta caagcccctc aggctatatc ccatgccgcg
    23401 atgagtatta atcggtgaaa gatcggggtt gatggcgcgc agcttggcac gcagcgtttt
    23461 aatgtggata tcgacggtgc gatcgtaggt gtcctgcgca tcttcccaca cgctatccat
    23521 tagttgctgg cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa
    23581 ctcataccgc gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt
    23641 caattcaaaa tgaccaatac ggatgacggg agacggcgtc gagaacttct tcacccgacg
    23701 cagtaaggtg cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc
    23761 gtcagcgcca atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa
    23821 cagtacaggt aacgccggat agagcgccag taactggcgg cacaattcaa agccactgat
    23881 atcaggcaaa ccaacgtcga gaatcatgac gtcgggaacc tgctggcgag ctttatccag
    23941 caccggcaaa cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac
    24001 cagcgtgtcg gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa
    24061 tcgcctctgt tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga
    24121 accttctaca actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tggcgtcata
    24181 aaagcgcacg acctgtaacg acttaaagac cagggacgtg cgttttttga atactacctc
    24241 gccctgagct ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca
    24301 agaaatggcc gcatcggagg tatcttctgc caggcccaat ccccctttaa taccaccggt
    24361 tttcgcccgg ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac
    24421 aacaattttg tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc
    24481 tgcatgagcc aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt
    24541 catattgtta ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata
    24601 ttgattaaga acaaaatctc aattctttta tttccgtcaa gccctaaaac atactctact
    24661 aagaaaaaaa cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc
    24721 aggtgctcgt tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac
    24781 cttttaatct ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg
    24841 aaagcaggtt attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct
    24901 attggcgcgt atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact
    24961 gcgcgtccga ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc
    25021 cgcgcattca agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg
    25081 agcgcctttg gtattcgccc gccgctacgt ctgggtgaat tcacaatgcc agagcacaaa
    25141 tttgtcaccc tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg
    25201 gagcaaatct ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc
    25261 aacgcgccga ccattccgcc ggtgctctac ggcctgaatg aaacgcgtcc gagtcaggat
    25321 aaagacgacg aacaagaggt attctatacc accgcgttag cccaggatca ggcagatggc
    25381 tatgtactga cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat
    25441 gaaggtctgg gaaccggcgt gcaggagttt atcctgacgg tatacggaac gtgcatgcca
    25501 atgctcaatc tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagac
    25561 gccaaagcgg gagatcgccc aattaatctg cgctgtgaac tgctgattcc tatccgccgt
    25621 taacgctgca gctcatctaa tgcaggggcg tccagatgcg agatgtcccc tgcggtttca
    25681 acgacccagc cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag
    25741 ttacgcagac gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga
    25801 catcccagtg caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg
    25861 caagattcca gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga
    25921 atacgcccgt caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc
    25981 agagaatcga tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca
    26041 aagatgatgt cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct
    26101 aaatcgctac tgataatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc
    26161 gcctgttgct cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg
    26221 gcgttccact gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt
    26281 atactgctgt aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga
    26341 ccaccaatcc cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag
    26401 gatcctgcca tattgcatcc gttgccgtcg agagcggtgt accggaacaa ccctttggca
    26461 gtgaggaaac gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag
    26521 aggctgattt ttgggtggcg attgaagctg gcattgatgg cgacagcact ttcagttggg
    26581 tggtgattga aaacgccagc cagcgcggcg aggcgcgttc ggcaactttg ccgttacctg
    26641 cggtgattct ggaaaaagtt cgtgaaggcg aggcgcttgg tcccgtgagt cgcgttatac
    26701 cggtattgat gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact
    26761 cactcgcgcc agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc
    26821 cgtgtatcag gcattgtagg acggataagg cgttcacgcc gcatccgaca ttcggtgcac
    26881 gatgcctgat gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca
    26941 acacctcttc cagccactga cgcagctcca cgggcgcggc tttcaggctg ttagatccac
    27001 gcgtaatcgt cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc
    27061 cacgcaacag ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg
    27121 tcagcatcag gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt
    27181 cgacaaaacg taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt
    27241 gttgggccat aatatgtcgc cattgttagc gggggaagca aaatgactcc cccggttgta
    27301 ctcgctaaag agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc
    27361 cgtggcgctc atcaacgtcg gtttatcccc catgaaatag cggtagtaag catcataagc
    27421 cagcacgttc ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac
    27481 agcgtcgata cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt
    27541 ataagctgct gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta
    27601 actggtgcca atgttgatgt tcgtttccgg atccagcaat tgcccagggc tgctataacc
    27661 aggaatagag aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc
    27721 gctggcccct accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc
    27781 catcgcatag ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta
    27841 agccagcggg aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac
    27901 gctaagatcc caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt
    27961 tgacttgctc ttcaccagat tggcccactc gctacgcgcg gtattatcga gattccagta
    28021 catcaactcg cgcacgcgcg ccatctccgg cccctgagtc agggtgctgt caacattctg
    28081 cggcgcttta tcaatcttca gctcatactc ttcaccgatg cgttgtgctg caaccatcgg
    28141 gtagaaacca cgctgttgta tgagttgatg caaaatctct ttcgcttcag cttcacgtcc
    28201 gcgttccagc aataaatccg cctgccagta acgccattca tctttctctt ttgcttccat
    28261 cggcagacgc gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gcgccattcg
    28321 cacacggcgt tcaatcaacg acgtggattg cgagcgcata atggcgtcat cgcgccattt
    28381 cgcctgctca tcggtgacat cattgcccat caaacgccag gcgacgatat cgcgcagctc
    28441 ctgaatctga tcttcattaa gctgctgcgc ctgggcaagc gacgggatca tcagccgtgc
    28501 attttcggca tcctgccgcg ccacactggc aaacgccacc gccgccattt gacgggtaaa
    28561 atcggtcgcg ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga
    28621 aatgattgcc gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac
    28681 caggcctgtg ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc
    28741 ttgtttacct gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt
    28801 gccggttagc cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca
    28861 tttcgcatag tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc
    28921 taacaagcca cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt
    28981 gcgagcggga ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg
    29041 attcatcagg tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt
    29101 cagtccaggc atcatttgtt cgaccacatc catctgtcga ttatcccagg cctgcttgat
    29161 ttgcgcgtaa cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt
    29221 cagcagacag acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag
    29281 cgcatcctct aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta
    29341 accagtcatt gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga
    29401 tcgaccaact gggattaggc ggggaaacag gctacacttc gcctttgaaa actcatcatc
    29461 acgataaaca tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc
    29521 ggcaaagttg ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct
    29581 ggggcaaaaa ttggtgtcct gggtctgaac ggcgcgggta agtccaccct gctgcgcatt
    29641 atggcgggca ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt
    29701 ggttatctgc cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa
    29761 gaagcggttt cagaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac
    29821 gccgatccgg atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc
    29881 attcaggctc acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgctg
    29941 cgtctgccag attgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgtg
    30001 gcgttgtgcc gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac
    30061 cacctggatg ccgaatccgt ggcgtggctg gaacgcttcc tgcacgactt cgaaggcacc
    30121 gtggtggcga ttactcacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa
    30181 ctggaccgcg gtgaaggaat tccgtgggaa ggtaactact cctcctggct ggagcagaaa
    30241 gatcagcgtc tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa
    30301 gagctggagt gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg
    30361 gcacgctttg aagagctgaa cagcaccgaa taccagaaac gtaacgaaac caacgaactg
    30421 tttattccac ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa
    30481 tcctacggcg atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcgatc
    30541 gtcgggatca tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctccggt
    30601 caggaacagc cggacagcgg caccatcact ttgggtgaaa cggtgaaact ggcatcggtt
    30661 gatcagttcc gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg
    30721 ctggatatta tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt
    30781 aactttaaag gggttgatca gggtaaacgt gttggcgaac tttccggtgg tgagcgcggt
    30841 cgtctgcatc tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca
    30901 accaacgacc tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg
    30961 ggctgtgcga tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc
    31021 ctggactacc aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa
    31081 gagtacaaaa aacgcacgct gggcgcagac gcgctggagc cgaagcgtat caagtacaag
    31141 cgtattgcga agtaatgcgt aaaatgccgg atgcggcgcg aacgccttat ccggcctaca
    31201 aaaccagcaa tttcaatacg ttgtaaaact gtaggcctga taagacgcgg taagcgtcgc
    31261 atcaggcatt gagcaccgaa tgccggatgc ggcgtgaaca ccttttccgg tctacaacag
    31321 ccttagcatc ctcaatttcg tggcgggtca cccgtgcagg ttttttcaaa ccttgtttaa
    31381 tctcgacagc ctcttccaga gaggttttca actcatcaaa aaaactcatt ttatacctcg
    31441 cctttgagca gtttcgtcac ggttatcctt gctcccccat catctcccgc accagctcca
    31501 cgcagcgcag gaaacggctg tcgtaatcgt cctcttcaac ccgcacgaat tcgatattgt
    31561 tctcttccag catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga
    31621 ggctgcgtaa accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga
    31681 aacggtattc atcaatcagc gcctgcacga acggatgctc acgcccttcg tactttttgc
    31741 agaacgcctg ggtagtgaca aaatcggtgt caataaacgc cactttattg gcgtatttca
    31801 ctgcaaaatc aatgtattgt gcgtgaccca acgcgatttt atcgtagtcg gaatactgca
    31861 atgcgatttc atcaccgccg aggtgtgaaa agacataatc gcggccatat tcccacgcac
    31921 tggtggtgtt gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc
    31981 cgccaaggat cgccacggta cgcacaaaaa acggcttcac ttcggtagga atatattccc
    32041 agtagcggaa cgggttttcg cggatctgcg caccgctgat actcataaag gtacgtttcg
    32101 gatcgaccag caccgtctcg atccccagat gttccatata ctgtggcgca tcggcttctt
    32161 ccgaggtgta gatcaggtcc ggctggatcc ccttttcagc cataaacttt ttgatgccgt
    32221 tgctccacac atcccagcca tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa
    32281 tacggatatt tttctgatat ttaaaggttt gcaatagcca gcgcagacga tccggcaccg
    32341 tgggctgctg cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac
    32401 ccataatgat atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc
    32461 cggtatgcag cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgccggggaa
    32521 attcaagccc caaaaaacgg tgcaatgcct ccagcttttg cgcgctgggg cttttaattt
    32581 tggcattcag taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct
    32641 gtagcgtgca gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc
    32701 cctccgtatc tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac
    32761 gccaaaaatc tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc
    32821 ccggcgaaaa ccgtgtttcg ccgcttcaga aatccgctcc tggccactgg gcaccgggcg
    32881 gatctcccct gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc
    32941 acgcaggctg gaaaccatcg ccagcagtaa cgctaagtcg gcgctggttt cggttacctt
    33001 cacgccgccg accacgttca caaacacatc ctgatcggcc atttgcagac caccgtgacg
    33061 gtgcaagaca gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg
    33121 cggattcgcc attatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt
    33181 tccttcccac accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa
    33241 aattgccgaa gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag
    33301 ctcattcacc gcgccgaagc ggtttttatg gctgcgtaag gtgcgaaaac gggagtcggc
    33361 atcgccatcc agaagcaccg aacagtcgat acagtgttcc agcactttcg ggccagccag
    33421 cgaaccatct ttggttacgt gtccaaccat gacaatcgcc acaccgcgcg ttttggcgaa
    33481 acgcgtcaga taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac
    33541 atccgccata tgcatcacct ggatcgagtc aattaccatc agcttcggtt gctcttcttc
    33601 ggcaatcagg cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg
    33661 caggccaagg cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata
    33721 cagcgttttc atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc
    33781 cgcaccaggg ttaccgccaa tcagaatggc acttcctggc accacgccgc ctcctagtac
    33841 gcggtcgaac tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga
    33901 gagtttctgg acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg
    33961 cgccaccgtt ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc
    34021 actgcactgc ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg
    34081 ttttggagct tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatgacgggc
    34141 aggcaattac ttctgattca ggctgccaga gaggatgcag aataccccca tcaggtcagc
    34201 gtgacggatg gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc
    34261 cagccctgct gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg
    34321 cgccagcggg atttcatact cctgcgcgag gcgagtcaga gttttcgctt tgtactgcgc
    34381 gtctacgatg tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt
    34441 ggctaccacg gctgtcaggc gcagcttgtc gcgcaggtat tcagcaaaga aagtaaagcc
    34501 gccggaggca atcgccactt tccagcccag cgtttccagc ttgagcacca gctgcgttaa
    34561 gcctggcatc agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt
    34621 cagcgtcgcc acgcggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg
    34681 ttcggttacc tccgccacca tctcgcccgt tccggccagc ttggcaattt catcaataca
    34741 ttcaatctgg atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg
    34801 cgggattttc cccagcgggg cgacatccag ctgcgcttcg tgggccaggc gtgtagcccg
    34861 tgcggtgagt gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac
    34921 aatcaccatc gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc
    34981 acgaccatac agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc
    35041 accacttaat gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca
    35101 ggtaatgtta ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga
    35161 ggctaccttg tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag
    35221 atggctcgca caaaactgaa attccggcta catcgggcag tgattgtcct gttctgtctt
    35281 gccttgttag tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag
    35341 cgtaatccac agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt
    35401 gcaccgctga tgcgtaccga ctcaccggat gaaaaacgca ttcaggcgat cctcgatcag
    35461 ttaacagatg aaagccgtat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc
    35521 gcacgttctg gcgaaagcgt cgaagtgcgc gatcggctgg cgctcgacgg taaaaaagca
    35581 ggcggctatt ttaaccagca gattgtcgag ccaattgcgg gtaaaaacgg accgctcggc
    35641 tatctgcgcc tgacactcga cacccatacg ctcgccaccg aagcccaaca ggtggataac
    35701 accactaaca ttttacgcct gatgttgctg ctctcactgg caatcggcgt agtgctgacc
    35761 cgcacgctgc tacagggtaa acgcacccgc tggcagcaat cgcccttcct gttaaccgcc
    35821 agcaaaccgg tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga
    35881 aaggaaatcg ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt
    35941 aacctggcgg tggaagagtg tatttttcgc caaatgcccg ccacgcagcg cgttctgttt
    36001 ctctggcgca atgccgacac ggtagtaatt ggtcgcgcgc agaacccgtg gaaagagtgt
    36061 aatacccggc ggatggaaga agataacgtc cgcctggcgc ggcgcagtgg cggtggcggc
    36121 gcggtgttcc acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac
    36181 gataaaacta tctccacgtc gattgtgctc aatgcgctga acgcgctcgg cgtcagcgcc
    36241 gaagcgtccg gacgtaacga tctggtggtg aaaaccgtcg aaggcgaccg caaagtctca
    36301 ggctcggcct atcgcgaaac caaagatcgc ggcttccacc acggcacctt gctactcaat
    36361 gccgacctca gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa
    36421 ggcattactt cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggctcacc
    36481 atgagcaggt ttgcgaggcc ataaccgagg cctttttcgc ccattatggc gagcacgtgg
    36541 aagcggaaat catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg
    36601 cccgtcagag tagctgagag tggaacttcg gtcaggctcc ggcattctcg catctgctgg
    36661 atgaacgctt tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca
    36721 cccgcgccca ggtatttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc
    36781 gactgcaagg ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg
    36841 ttgacttccc ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg
    36901 taaggtaact cgctgattaa aatatccaag gaaggaaata tgatcgatct ggaaaaccag
    36961 gaacgggaga ttatcaattt aatgttcagc cagcgtattt cctggctagc cgccgtgcgt
    37021 atcaggcata aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct
    37081 ctaaaacaga tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatggcagaa
    37141 atttatggtt gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa
    37201 tgaatagatt cgtgaataga tacttgaatg ttttttattt tctatccata tgaaaaataa
    37261 tagtaaatct ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc
    37321 ccactccggc ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca
    37381 gcagaacgga ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg
    37441 cagtggtctg ctcgtgagtg cggatgtggt cagacacggt gcagatggtc agggctttcg
    37501 cgccaaactc tgcagccacg ccgtagatac cagccgcttc catttccacg ccgaggatgc
    37561 cgtatttttc catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga
    37621 acaggttacc cacgcgagca tcaacgccca gtgctttagc tgcatctact gcgttacgca
    37681 ccatgtcaaa gtcagcgata gcggcaaagt catggtcttt aaaacggatg cggttaactt
    37741 tggaatcggt gcaggccccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa
    37801 ctgcgccaca ggaaccaacg cggataattt tcttcacgcc gaaatcggtg atcagttctt
    37861 tggtgtagat ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc
    37921 ctttgtaagt accagtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt
    37981 caaggaaagt ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt
    38041 cagcgaaatc gcccatttct gcattaatgt gtggggtagc cattgtttta tcctttttgt
    38101 gacataacaa aggcggagtc aacgctccgc ccaaatccat cagaacatgg ctttgccata
    38161 ttccatatca gaagtaccaa aatattttgc cagagtctgg ccgatatccg cgaaggtttc
    38221 acggtgaccc agtgagcccg gttttacttt cgggccatat accagtaccg gaatgtgttc
    38281 acgcgtgtgg tcagtaccgg tccaggtcgg atcgcaaccg tggtcagcgg tgaggatcag
    38341 gatgtcgtca tcgcgcagca gagacatcag ctccggcaga cggcggtcga acagttccag
    38401 acccgcagca taaccggcga cgtcgcggcg gtggccccag gaagagtcga agtcaacgaa
    38461 gttggtgaag acgatggtgt tatcacccgc ttctttcatc tctttgatgg tggcgtcaaa
    38521 cagcgcgtcc aagccagtcg ctttcacttt tttggtgata ccgcagttgg cgtagatgtc
    38581 tgcaatttta ccgacagaaa ccacctggcc gtgtttttca tcaaccagtt tctgcagcac
    38641 ggtcggtgct ggcggctcaa cagccaagtc gtgacggtta ccggtacgct ggaagttacc
    38701 agctttgtca ccgataaacg gacgagcgat aacacgaccg atattgtagc cgccgttggt
    38761 cagctcttca cgggcgattt cgcacagttc gtagagttta tccagaccga aagtttcttc
    38821 atggcaggca atctggaaca cggagtcagc ggaggtatag aaaatcggct tgccggtttt
    38881 catgtgctct tcgcccagtt gatccagaat gaccgtaccg gaagagtggc agttaccgag
    38941 gtaacccggc agattagcgc gttcgaccag tttatccagc agctcttgcg ggaagctgtt
    39001 ttcgtgatcg gagaaatatc cccactcaaa cagaaccggg acaccggcga tttcccagtg
    39061 accagacggg gtatctttac cggatgacat ttcgtgcgcc catgcgtacg cgccgataac
    39121 ttcagcgttg ccgtccattc ccgccggaat gaaaccggta gaaccttcgt gtgctttcgc
    39181 cagccccaga cgggtcagat ttggcagatt gagcggccct ttacgaccat tatcagcttc
    39241 gcctttggca caagcttctg cgatatgacc cagggtgtca gccccgacgt caccaaagcg
    39301 ttctgcatct tctgtagcgc cgatgccgaa tgagtccagc accataataa atgcacgttt
    39361 catatgttct ccgtactcag tgcttcaaat ttaaatgatc agatcagtat accgttattc
    39421 gctgatacgg cgatagacag ttggtgtgct ttccggtgcg ttatcggcaa gtttaattgc
    39481 cgctttcacc gctttcgccg cttcctgcca gctgttttcg tctttcgcga ggataaccgc
    39541 cagcggacgc tgaccgtcta cctggtcgcc cagacgcgcc atatcagtaa agccgacgct
    39601 gtaatcgatg gtgtcagatg cctgacgacg accgccgccc attgcaacta ctgccatccc
    39661 cagcgcgcgg gtatccattt cactgacaaa accttcggta tcagcataga ctgctttcgt
    39721 cagcatcgct gtcggcagat acttcgcgta gttctcaacg aagtcggtcg ggcctttttg
    39781 tgccgctacc atacgaccaa agacttctgc cgctttaccg ttgtccagca ccgcctgcaa
    39841 tttcgcgcgc gcttcggcgt catctttcgc cagtttgccg gagatcaaca tctccacgca
    39901 cagcgccatc gtgacatcaa acagacgcgg gttacgatac tcacccgtca ggaactgcac
    39961 cgcttcacga acttcaaccg cgttacctgc actggaggcc agtacctgat tcatgtcggt
    40021 gagcagcgcg gtggtgcgca cgccagcgcc gttagccacg ccaacaatcg cttcggcaag
    40081 ggcttcagag agttcgtagg tcggcataaa cgcgccgcta cccactttca cgtccatcac
    40141 cagcgcgtcc agaccttccg ccagtttctt cgccaggatc gaggcagtga tcagcgggat
    40201 ggagtccacg gttgcggtaa tatcacgggt cgcgtagaaa cgtttatcag ccggagccag
    40261 tgaactggtc tgaccgataa tcgccacgcc gacgtcttta ataatttcgc ggaaacggtt
    40321 gtcatccggg aaaatgtcga agccagggat ggattccagt ttgtcgagcg taccgccagt
    40381 atgaccgagg ccgcgaccgg agatcatcgg aatatagccg ccgcaggctg cgaccatcgg
    40441 ccccaacatc agcgaagtga catcgccgac gccgccggtc gagtgtttat caacaatcgg
    40501 gccattcaga tgcaggcttt tccagtcgag aacggttcct gaatctcgca tcgccatggt
    40561 cagcgagaca cgctcaggca ttgtcatatc gtggaagaaa atggtcatcg cgagggcggc
    40621 aatctgccct tcggaaatag tgttgtcgcg aataccgttg ataaagaaac gaatttcttc
    40681 atcgctcagc gcatgaccat cacgtttttt acgaataatt tcttgtgcga gaaacaaggt
    40741 aacctcccga ggaaaagagt atgaggcggc ttttgccgga tgcgtctacg ccttatccgg
    40801 cctacgggta gatacgtctc gtaggcctga taagcgcagc gcatcaggcg taaagcatct
    40861 tacttagtag ctgctggcgc tcttaccgtc gccgtggccc agcgctttca gcaggcttgc
    40921 cagcaggctg gaagcaccaa agcggtagtg acgcgcatca gcccagtcag caccgaacag
    40981 ttcatctgcg atggcgagat atttctgcgc atcttccgca gtacgcacgc cgcccgccgg
    41041 tttgaaacca acggtttttt ctacgcccat atcacggatc acttccatca tgatgcgagc
    41101 gctttccggc gtcgcgttca cagccacttt accggtagag gttttgatga agtccgcacc
    41161 cgctttgatg gagatttcag acgctttacg gatcagcgct tcgtctttca gttcgccggt
    41221 ttcgatgatc actttcagca gtacattcgc tgccgcgcaa gcctctttac aggctttcac
    41281 caggtcaaaa ccaacctgct cgtcacccgc catcagcgcg cggtacggga acaccacgtc
    41341 aacttcatca gcaccgtagg cgattgccgc acgggtttct gccagcgcga tttcgatgtc
    41401 gtcgttaccg tgtgggaagt tggttaccgt agcgatacgg atttccgggg tgccctgctc
    41461 tttcagagtt ttgcgagcaa tcgggataaa acgaggatag atacagatag cggcggtatt
    41521 gccgaccgga gttttggcct gatgacacag agcaattact ttctcgtcgg tgtcgtcgtc
    41581 attcagggtg gtcaggtcca tcaatttcag tgcacgcagg ctgcttgctt tcagatcagt
    41641 catttcattc tcctggcttg tcgccaataa aattcacctt gcgagtttgt tagtattcta
    41701 acatctactc cgcaacacac ttcgatacac atcacaatta aggaaatcta cttacaagtt
    41761 tgcatcactg taatgcgatc tggttcaaat aattcacttt caaatgaatg cgtcagtggt
    41821 ggcaaacgca tcaggatcaa agtgaacatc acgaaacttc ttacaatggc gcatcacctt
    41881 cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa tgcactgcaa caacgttgcc
    41941 agcaaattgt caccagccca gtgcttagcc cggagcagaa gcgccatttt ctggcactgg
    42001 aagcagaaaa caatctgcct tacccacagc ttcctgccga agcccgccgc gcgctggatg
    42061 aaggtgtaat ctgcgatatg tttgaaggtc atgcgccgta caaaccgcgc tatgtcttac
    42121 ccgattacgc tcgttttctg gcgaacggtt ccgaatggct ggagctggaa ggcgcgaaag
    42181 atcttgatga cgcactctct ctgctgacca ttctttacca ccacgtaccg tcggtcacat
    42241 cgatgccggt ctacctgggg caactggatg cgttgttgca accgtatgtt agaattctaa
    42301 cacaaaacga gatcgatgtt cgaataaaac gtttctggcg ttacctcgac agaaccctgc
    42361 cagatgcctt tatgcacgcc aatatcggcc cgtctgattc gcccattacc cgcgcgatct
    42421 tacgtgcaga cgcgcaggtt tcaccgaacc tgacctttat ctacgatcct gaaatcaccc
    42481 ctgatgacct gctgctggaa gtggcgaaga acatctgtga atgtagcaaa ccgcacatcg
    42541 ccaacggtcc ggtgcatgat aaaattttca caaaaggggg ctacgggatt gtgagctgtt
    42601 acaactcact gccgctggcg ggtggtggca gcacgctggt acgccttaac ctgaaagcca
    42661 ttgccgagcg cagcgaatcg ctggatgact tctttacgcg cactctaccg cactactgcc
    42721 agcagcagat cgccatcatc gatgcgcggt gtgaattcct ctatcaacaa tcacacttct
    42781 ttgagaatag cttcctggtg aaagaagggc tgattaaccc tgaacgtttt gtgccaatgt
    42841 ttggcatgta tgggctggcg gaagcggtta acttgctgtg tgagaaagaa gggattgccg
    42901 cgcgctacgg taaagaagcc gccgcaaatg aagtgggtta tcgcatcagc gcgcaactgg
    42961 cggagtttgt cgccaatacc cccgtgaaat atggctggca aaaacgcgcc atgttacacg
    43021 cacagtcggg gatcagttcc gatatcggca ccacgccggg cgcgcgtttg ccgtatggcg
    43081 atgagccaga tccgatcacc catctgcaaa ctgtcgcccc gcatcatgct tattattatt
    43141 ccggcatcag cgacattctg acgctcgacg aaaccatcaa acgtaacccg caggcactgg
    43201 tacagctttg cctcggtgcc tttaaagccg gaatgcgtga atttaccgcc aatgtcagcg
    43261 gtaacgatct ggttcgcgtt accggttata tggtgcgttt gtcggattta gaaaaatatc
    43321 gcgccgaagg ttcacgcacc aacaccacct ggctgggcga agaagccgca cgcaacactc
    43381 gtattctgga acgccagccg cgcgtgataa gccatgaaca gcagatgcgc tttagtcagt
    43441 aagattatcc ccttctcctg cgttgacggg ccaggcagtc gtctggctct gtttttgcag
    43501 ggctgcaatc tgcgctgcaa aaactgtcac aatccgtgga cgatgggacg ttgcaatgac
    43561 tgtggggagt gcgtgccaca gtgtccgcat caggcgttgc agattgttga cggcaaagtg
    43621 gtgtggaacg ctgtggtctg cgagcaatgt gatacctgcc tgaagatgtg tccgcaacat
    43681 gccacaccaa tggcgcaatc catgagcgtg gacgaagtgc ttagccatgt gcgcaaagcg
    43741 gtgctgttta ttgaagggat aacggtaagc ggcggcgaag ccacgaccca actgccgttt
    43801 gtagtggcgc tgtttactgc tatcaaaaac gatccgcaac tgcgccatct cacctgtctg
    43861 gtggacagta acggtatgtt gagcgaaacc ggctgggaaa aattactccc ggtgtgtgac
    43921 ggcgcaatgc tcgatctcaa agcatggggt agcgaatgtc atcaacaatt aaccggacgc
    43981 gataatcaac agattaagcg cagcatctat ttgctggcag agcgcggcaa gctggcggaa
    44041 ctgcgtttgc tgatgatccc aggccaggtg gattatttgc aacacatcga agaactggcg
    44101 gcgtttatca agggacttgg tgatgttccg gttcgcctga acgcgtttca tgcccacggc
    44161 gtgtatggcg aggcgcaaag ctgggcgagc gccacgccgg aagacgttga gccgttggct
    44221 gatgcattaa aggtgcgcgg ggtgagccgg ttgatatttc cggcgctcta tttgtgacat
    44281 gccggatacg gcctacggtt cggcacagac ttgtaggcct gataagacgc gtcaagcgtc
    44341 gcatcaggca ttgtgcgcca actgccggat gcggcgtgaa cgccttatcc ggcctacggt
    44401 tcggcacaaa cctgtaggca tgataagacg cgttaagcgt cgcatcaggc attatgcgcc
    44461 gactgccgga tgctgcctac gaagggcaaa ctacccacga acgttaaaca ccgcatacgt
    44521 gttattaagc aacacttcgg caatctcatc cgcgggttcc gggcgtaact cgcaaagcac
    44581 ggcaaacaca cgggcagcct gttccgggcg gttaggctgc ccctgaaaac cgttgagcgg
    44641 catatccggc gcatcggttt ccagcaacaa cgatgccagc ggtaattttg cgatgacatc
    44701 gcgggtttta ctggcgcgtg gataggtgat agtaccgcct acgccgattt tgtagcccag
    44761 ctgtacaaac cgttcagcct gttgctggct gccagaaaat ccgtggacaa caccagcacg
    44821 cggcaaatcg tggcgtttaa gatgcatcgc cagtttgtcg tgcgtgcgcc gtgaatgcag
    44881 gatcaccggc agatcgtagc gtttcgccag tttcagttgt tcgtcgagta accactgctg
    44941 cctctcaaat tgcggatcgt cgccgaagag atccagaccg atctccccca ccgccaccac
    45001 cttcgccgga cgcctttcca gcgcctgctg tagctgctca agagacacat cgctatgttt
    45061 ttccaacata ccaggatgca agcccaatac ggcatacagc ggttgataat tttccgctaa
    45121 cgcctgcaca cgggcaaaat tttccgcctc tgtcgccgga acaatgatct tgcctacgcc
    45181 cgcttgtgcc gcgcgttgca ggctggcctc ttcatcgcca ataaaaggcg ggaaatcaaa
    45241 atggcagtga gtgtcgataa aacggcaaat caagccagat cctcattgtt aaatgtcggg
    45301 tcgttcgcct gcggagcgtc gctcacttcg gcaaccagcg tatcgttggc gactggcgca
    45361 ggcggaatga caatcgattc cggcgtcacc actggcacca gatggcgggt aagcggcgct
    45421 ttttcagtta atagcttgcc aaccgtggcg aggaaataac gaccgcataa acgcccaagt
    45481 ttatagtctt cacgcagcgc cggaatccga ctgccaagcg cgatactatg taatggcttc
    45541 ggcggataaa tttcggatat ccgcagcttg cccggtggtt tctcaataaa ttgctgaatg
    45601 tcacgatagc tggtttcatg atgctgcacc agattgacca gcggctgcag gctactgtca
    45661 cccagccagc gttccatgcg tttgaaccac tgcggcgtgt agtacatttg tgacggcaca
    45721 gtgcggatga cgaccaacgt tttagcgccc tgccttgccg cctctttaac cggaatcgca
    45781 tcactgatcc cgccatccag gtagttaatg ccttccagcg acactccgct acgataaaag
    45841 ccaggtatcg cactggaggc gcgaatcaca tccagccagt tttgtttggt tggtaaaaag
    45901 taattcggcg cgtagtcatc ctgacgacag gcgcacatat aaaacgattt gccgctgtca
    45961 aacaaccgcg cggcggtatc catttgcaac ggcatctggc ttgcggtggc ctccaccagc
    46021 cagtcgagat cgataagatt tcctccacgg acaaagcgca atggatcgaa aaattcgcgt
    46081 tttgtggtat agcgcatgat gactttgcgc gcgtaaccgg gctgattgca gataaacgcc
    46141 gagaggttct gcgccccggc agatgtgccg agataaagat cgaaaggatt aaactgcgcg
    46201 cgcataaact catccagcac gccagccgtg aaaattccac gctgtccgcc gccttcgcac
    46261 accagagcta ttcgtccagg ctggaacggc cttagcgaca acggcgcaat attaccaagc
    46321 gttacaggta ttcgctgccc cacttctgcc ttcctgtttt tgttgtgaaa gaaagacaaa
    46381 gtaacgcaat ttattattcg ctaaaaccgc aaaagccagt ccgctggact ggctttggat
    46441 aaaattttct taatggtctg aaatctaggg tcgttttcgg cccatgaaca aactcactag
    46501 gaacagaata atcccgacga caaagacaat tttagctgcg cctgcagcgg taccggccag
    46561 accaccaaac ccaagtgcgg cggcgattaa cgcgataacc agaaatatga tgccccaacg
    46621 aaacataagc gtctccttta ccatagttaa tgtcaccgct tgatatgagc gtgaaaatca
    46681 ctcaatgcga tatttttagt gtggtgcaca tcgcgcctcc cgacaaagtt cgggaggacg
    46741 aattacttag ttttcagatc atttttaacg cttttcacac catctaccgc tttggcgata
    46801 ctttcagcac ggtcactttg tgcctgagaa tctacggtac cggagagctg aaccacgcca
    46861 tcggtggttt caactttcac tttacgtgaa gggacgatat cgtccgccag cagtttggct
    46921 ttgatttcac tggtggtggc ggtgtcaccc gcgtagccct tcaccgagcc ttctttagcg
    46981 tcgcgaacgt gcagtttgtc gctgacagag gtcacgcctt caacgccttt cgccactttc
    47041 actgcctctt cggcctgggc ctgactttca acgaaaccgc tcagggtcac gactttttga
    47101 tcggttttga cagagatatc ggtgctcttg atgttgtcat gatccaccag cgccgccttc
    47161 actttcgcgg tgatggcgct gtcatccatg aaattaccga ctttattcat agagctatcg
    47221 actttttgcc ctgcgctttc attggtagtc tgcgcgttgt tttccgcgta ggcagagccg
    47281 gtcgcgacgg cagaggtcaa cattacagcc agcagagttt tcgaaatctt cagtcttgtc
    47341 atagtcatcg atttattcct gtatgtttgc tcgtaatttg agcctagcaa cacgaggttg
    47401 cattgctgaa tagggagaga cttcaccctc tacagaagtc aatggtcgcc atcacaaaag
    47461 cgatgggtga tgaataacga ccattacagc ctctgaatca gttattaata tcggtagaat
    47521 gacaatcgac ggctttagat actaatatct acgcattgaa cggtatttaa cgccatcaga
    47581 aatgtcatca ctttgttaaa tatagatcac aattttgaaa ccgctcggga tatcagcgaa
    47641 aacataagca aaagtgaatg ttttaagaac attccgtaag cggctaataa ggaagggaaa
    47701 ttgacagggc gcagcggcta ccgcgccctg gaggcaagaa ttaatgctcg cgggtctggt
    47761 ggaactgaac gtccggataa cgttcctgtg ccaggcgcag gttgaccatg ctggtagcga
    47821 tgtaagcgag gttatcgccg ccatcaagcg ccagttggct ttcgttctta cgcttgaact
    47881 cttcgaattt cttcgcgtct gcacattcta cccagcgggc agtggcaacg ttgactgact
    47941 catacactgc ttcaacgttg tattcgctct tcaggcgcgc taccaccaca tcaaactgca
    48001 gcacaccaac cgcaccaacg atcagatcgt tgttggagat tggacggaac acctgcaccg
    48061 cgccctcttc ggaaagctgt accagccctt tgagcagctg tttttgcttc agcggatctt
    48121 tcaggcggat acgacggaac agttctggtg cgaagttcgg aataccggtg aacttcatca
    48181 tctcaccctg ggtaaaggtg tcgccgatct gaatggtgcc gtggttgtgc aggccgagga
    48241 tatcgcccgg atacgcttct tcaacgtgcg aacggtcacc cgccataaag gtcagcgcgt
    48301 cggagatcac cacatctttc gcagtgcgca cctggcgcag tttcatgcct ttttcatatt
    48361 taccggacac cacacgcata aacgccacgc ggtcgcggtg tttcggatcc atgttggcct
    48421 gaattttaaa tacgaagccg gtaaatttat cttcgctcgc ttctacggta cgggtatcgg
    48481 tctgacgcgg catcggcgca ggcgcccact ccaccaggcc attcagcata tgatcaacgc
    48541 cgaagttacc cagcgcagta ccgaagaata ccggagtgat ttcgcccgca aggaacagct
    48601 ctttgtcgaa ctcgttagac gcgcctttaa ccagttccag ttcgtcacgc agctgctgtg
    48661 ccagatcttc accaaccgca gcatcgagat ccgggttatt cagcccttta acaatgcgga
    48721 cttcctgaat ggtgtgccct ttaccgctct gatagagata ggtttcatct ttataaaggt
    48781 ggtaaacgcc tttaaacagc ttgccgcagc caattggcca agtgatcggt gcgcagccaa
    48841 ttttcagctc gttctcaact tcatcgagca gttccatcgg gtcgcggata tcacggtcaa
    48901 gtttgttcat aaaggtgagg atcggcgtgt cgcgcagacg ggtaacttcc atcagcttac
    48961 gggtacgatc ttcaacacct tttgcggcgt cgataaccat caggcagcag tccaccgccg
    49021 tcagggtacg ataggtatct tccgagaagt cttcgtgccc cggggtgtcg agcaggttaa
    49081 ccaggcaatc gtgatacgga aactgcatca cagacgtagt aatggagatc ccacgctgct
    49141 tttccatctc catccagtcc gacttagcgt gctggttgga accacggcct tttactgtac
    49201 cggcggtctg aatggcctgt ccgaacagca gcaccttctc ggtgatggta gttttaccgg
    49261 cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc cacctctttc aaataaggag
    49321 acaacgtcat aattctttct tcttgagtaa gcggcatcgc gccgcgcttg ttgaaaacga
    49381 aaaattgcgg ctattttacc catcaacggg ggggaggcaa tcagtgttta cacaggagct
    49441 gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc gatgccttct ggttgctcgc
    49501 gattgtgcgc attcagccag caggtcgcaa gcccggcgtt gatgccaccg agaatatcgg
    49561 actcggcagt gtcgccaacc atcagcacgc gtgaacggtc aggattgccc gcctgttcca
    49621 gcgcataatc gaaaattttc ttattcggtt tggcaacgcc aacttcttcg gaaatcacca
    49681 gcaaatcgaa gtaatcacgc aggcccgtcc gttccagacg cacttgctgc aaggcgctaa
    49741 aaccgttggt gatgatgccg attttagcgt tgctacgaat ggcgttaagc agagaaaccg
    49801 cgcccggcaa cggcgtacag atttccgcca tcgcattaat aaaggcatcg ttaagcaagc
    49861 ctgcttcaac tttcagccgt tcggcccagc tctcgaaacg cccgtgctga agctgtaatg
    49921 aagtgatcgc gccgttttga taatccaccc acagtggctt gttaacggcc tgatagtcct
    49981 gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa catccgctgc aggccggtga
    50041 atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa aatccagtcc cacttcatta
    50101 ttccaccttg tcttacatac tgattggcaa cgccatgatg atggcgtctt cgcgaccgtc
    50161 cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta aagcctaaac tttcgtacag
    50221 ggcaatggcg gcagcatttg aggcacggac ttccagccat agtgtcgcca cgccgcgttt
    50281 ttccagttcg tcgatcagat gttccagcag cgccctaccc agcccctgac gctgatagtc
    50341 gggatcgacc gcaatattga acaatgtagc ttcatccagc accacttgcg taatcgcaaa
    50401 cgccgccatt ttgccgtttt gcgttaactg aaagttgaga taacgctcgc cctggttgct
    50461 ggcaaaggtt ttttcactcc acggaaaggc gtgggcgcgt tgttcaatgt ggtaagccgc
    50521 tggtaaatca gtcgtttcga gggaagaaat cgtgttcata tgtgcaaatt tgttgccata
    50581 acgcggcgcg tgccgttggg tttgcccgta attcggtgag cgccggtgat gccacctgag
    50641 cgccttccaa tgatagcggt tcgtcagtac ccaaccgcca gctgttgcag cgactgcctt
    50701 gcggcagcat cgcgattttt tctggcgtca gttgcagcac ttgatcgggg ctgacggtta
    50761 atgcgcgcag aacatcgctc actaaaggat cagtcagggc gggaagatcg tttgccacca
    50821 tcaccagacg gacgtgtgcc gggctcgcaa tggcaatctc cccctgcaac gcgccagggc
    50881 gacgcagcga ccactgggta atgcccagtt gctgtaactg ccagtctcgt cgggatgtca
    50941 tagcgaatcg ctcctgttgt caggggcgca aatatagcaa attcgtcgat accgcgccaa
    51001 catatggcta taatcgccgc cagtatcaat tgaggagcat tccatgtctg catttacccc
    51061 ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa caaagccgta ttctgtttgc
    51121 cggagactta caggatgacc tgcccgcgcg tttagatacc gcggccagcc gtgctcatac
    51181 ccagcaattc caccattggc aggtgttaag ctgccagatg ggggataacg cccgttttag
    51241 tctggtcgcc acggcgaatg atgtcgcaga ttgcgatacg ctgatttact actggccgaa
    51301 gaacaaaccg gaagcccagt tccagttgat gaatttactt tctctgctgc cagtggggac
    51361 agatattttt gtcgttggcg agaaccgcag cggcgtgcgc agcgctgagc agatgctggc
    51421 agattatgcg ccgttgaata aagtcgacag cgctcgtcgc tgtggcctct attttggtcg
    51481 tctggaaaaa cagccagtat ttgatgcgga taaattctgg ggcgaataca gcgtcgatgg
    51541 cctgacggtc aaaacgctgc ctggcgtgtt tagccgcgac ggtctggatg tcggtagcca
    51601 gttgctgctc tcgacgttaa ctccgcacac gaaaggtaaa gtgctggatg tcggctgtgg
    51661 cgcgggtgta ctttcagttg cctttgcgcg ccattcgccg aaaattcgtc tcaccttgtg
    51721 cgatgtctct gccccggcgg tagaagccag ccgcgcaaca cttgcagcca acggtgttga
    51781 aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa ggttgttttg atatgatcat
    51841 ctccaacccg ccgttccacg atgggatgca aaccagcctg gatgcggcgc aaacgctgat
    51901 tcgtggcgcg gtgcgtcatc ttaatagcgg cggcgagctg cgaattgtag cgaacgcctt
    51961 cctgccttac ccggacgtgc tggatgagac atttggcttc catgaagtta tcgcgcaaac
    52021 cgggcgcttc aaggtatatc gcgccattat gacccgccag gcgaagaaag gttgagtatc
    52081 tgatatctgt cgccgcatac cgtgtcggat gcggcgtgaa cgccttatcc gacctacaaa
    52141 acctgcacgt tagcccttcg taggccagat aagacgcgcc agcgtcgcat ctggcatctg
    52201 cccccgcatg cctaccaaac ctgcacgcta gtttcctgat ggacattttt ccagcaatta
    52261 cacatcaggc gataattaac tattgacgaa aagctgaaaa ccactagaat gcgcctccgt
    52321 ggtagcaatt ctttttaaga attgatgg
//
LOCUS       DAJTUP010000017        49183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTUP010000017 DAJTUP010000000
VERSION     DAJTUP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.017"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..862
                     /locus_tag="OO468_001028"
     CDS             <1..862
                     /locus_tag="OO468_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6258232.1"
                     /translation="AAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIALLAC
                     AGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYVNTKG
                     MSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNFKVPF
                     NFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETVLNLP
                     DIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKYLLAQ
                     FGVDIGEAAQ"
     gene            859..1824
                     /gene="yggF"
                     /locus_tag="OO468_001029"
     CDS             859..1824
                     /gene="yggF"
                     /locus_tag="OO468_001029"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR6258233.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            1846..2355
                     /gene="fumE"
                     /locus_tag="OO468_001030"
     CDS             1846..2355
                     /gene="fumE"
                     /locus_tag="OO468_001030"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR6258234.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2352..3065
                     /locus_tag="OO468_001031"
     CDS             2352..3065
                     /locus_tag="OO468_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR6258235.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3037..3714)
                     /locus_tag="OO468_001032"
     CDS             complement(3037..3714)
                     /locus_tag="OO468_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6258236.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(3708..4385)
                     /locus_tag="OO468_001033"
     CDS             complement(3708..4385)
                     /locus_tag="OO468_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR6258237.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4373..4756)
                     /locus_tag="OO468_001034"
     CDS             complement(4373..4756)
                     /locus_tag="OO468_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR6258238.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(4772..5080)
                     /locus_tag="OO468_001035"
     CDS             complement(4772..5080)
                     /locus_tag="OO468_001035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258239.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5081..5659)
                     /locus_tag="OO468_001036"
     CDS             complement(5081..5659)
                     /locus_tag="OO468_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR6258240.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(5682..6113)
                     /locus_tag="OO468_001037"
                     /pseudo
     CDS             complement(5682..6113)
                     /locus_tag="OO468_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6485..7504
                     /gene="epd"
                     /locus_tag="OO468_001038"
     CDS             6485..7504
                     /gene="epd"
                     /locus_tag="OO468_001038"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR6258241.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7554..8717
                     /gene="pgk"
                     /locus_tag="OO468_001039"
     CDS             7554..8717
                     /gene="pgk"
                     /locus_tag="OO468_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR6258242.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            8932..10011
                     /gene="fbaA"
                     /locus_tag="OO468_001040"
     CDS             8932..10011
                     /gene="fbaA"
                     /locus_tag="OO468_001040"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR6258243.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10202..11062
                     /gene="mscS"
                     /locus_tag="OO468_001041"
     CDS             10202..11062
                     /gene="mscS"
                     /locus_tag="OO468_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR6258244.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11201..11836
                     /gene="argO"
                     /locus_tag="OO468_001042"
     CDS             11201..11836
                     /gene="argO"
                     /locus_tag="OO468_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR6258245.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            11929..12669
                     /locus_tag="OO468_001043"
     CDS             11929..12669
                     /locus_tag="OO468_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR6258246.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            12846..13796
                     /locus_tag="OO468_001044"
     CDS             12846..13796
                     /locus_tag="OO468_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6258247.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(13738..15216)
                     /gene="scpC"
                     /locus_tag="OO468_001045"
                     /pseudo
     CDS             complement(13738..15216)
                     /gene="scpC"
                     /locus_tag="OO468_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15240..16025)
                     /gene="scpB"
                     /locus_tag="OO468_001046"
     CDS             complement(15240..16025)
                     /gene="scpB"
                     /locus_tag="OO468_001046"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR6258248.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16036..17031)
                     /gene="meaB"
                     /locus_tag="OO468_001047"
     CDS             complement(16036..17031)
                     /gene="meaB"
                     /locus_tag="OO468_001047"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR6258249.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17024..19168)
                     /gene="scpA"
                     /locus_tag="OO468_001048"
     CDS             complement(17024..19168)
                     /gene="scpA"
                     /locus_tag="OO468_001048"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR6258250.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19372..20265)
                     /gene="argP"
                     /locus_tag="OO468_001049"
     CDS             complement(19372..20265)
                     /gene="argP"
                     /locus_tag="OO468_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR6258251.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20648..21307
                     /gene="rpiA"
                     /locus_tag="OO468_001050"
     CDS             20648..21307
                     /gene="rpiA"
                     /locus_tag="OO468_001050"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR6258252.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21563..22795
                     /gene="serA"
                     /locus_tag="OO468_001051"
     CDS             21563..22795
                     /gene="serA"
                     /locus_tag="OO468_001051"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR6258253.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23024..23083
                     /gene="ibsC"
                     /locus_tag="OO468_001052"
     CDS             23024..23083
                     /gene="ibsC"
                     /locus_tag="OO468_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR6258254.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23416..23475
                     /locus_tag="OO468_001053"
     CDS             23416..23475
                     /locus_tag="OO468_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6258255.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23576..24178)
                     /locus_tag="OO468_001054"
     CDS             complement(23576..24178)
                     /locus_tag="OO468_001054"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR6258256.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24199..24382)
                     /gene="ssrS"
                     /locus_tag="OO468_001055"
     ncRNA           complement(24199..24382)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO468_001055"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24424..24753)
                     /gene="zapA"
                     /locus_tag="OO468_001056"
     CDS             complement(24424..24753)
                     /gene="zapA"
                     /locus_tag="OO468_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR6258257.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            24921..25499
                     /gene="ygfB"
                     /locus_tag="OO468_001057"
     CDS             24921..25499
                     /gene="ygfB"
                     /locus_tag="OO468_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR6258258.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25525..26850
                     /gene="pepP"
                     /locus_tag="OO468_001058"
     CDS             25525..26850
                     /gene="pepP"
                     /locus_tag="OO468_001058"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR6258259.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            26847..28025
                     /gene="ubiH"
                     /locus_tag="OO468_001059"
                     /gene_synonym="visB"
     CDS             26847..28025
                     /gene="ubiH"
                     /locus_tag="OO468_001059"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR6258260.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28048..29250
                     /gene="ubiI"
                     /locus_tag="OO468_001060"
                     /gene_synonym="visC"
     CDS             28048..29250
                     /gene="ubiI"
                     /locus_tag="OO468_001060"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR6258261.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29324..29407)
                     /locus_tag="OO468_001061"
     CDS             complement(29324..29407)
                     /locus_tag="OO468_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258262.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            29698..30792
                     /gene="gcvT"
                     /locus_tag="OO468_001062"
     CDS             29698..30792
                     /gene="gcvT"
                     /locus_tag="OO468_001062"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR6258263.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            30816..31205
                     /gene="gcvH"
                     /locus_tag="OO468_001063"
     CDS             30816..31205
                     /gene="gcvH"
                     /locus_tag="OO468_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR6258264.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31324..34197
                     /gene="gcvP"
                     /locus_tag="OO468_001064"
     CDS             31324..34197
                     /gene="gcvP"
                     /locus_tag="OO468_001064"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR6258265.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34464..35207
                     /locus_tag="OO468_001065"
     CDS             34464..35207
                     /locus_tag="OO468_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6258266.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35264..36697)
                     /gene="bglA"
                     /locus_tag="OO468_001066"
     CDS             complement(35264..36697)
                     /gene="bglA"
                     /locus_tag="OO468_001066"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR6258267.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            36742..37052
                     /locus_tag="OO468_001067"
                     /pseudo
     CDS             36742..37052
                     /locus_tag="OO468_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37216..37875
                     /locus_tag="OO468_001068"
     CDS             37216..37875
                     /locus_tag="OO468_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR6258268.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38071..39051)
                     /gene="ygfZ"
                     /locus_tag="OO468_001069"
     CDS             complement(38071..39051)
                     /gene="ygfZ"
                     /locus_tag="OO468_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR6258269.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39294..39560
                     /gene="sdhE"
                     /locus_tag="OO468_001070"
     CDS             39294..39560
                     /gene="sdhE"
                     /locus_tag="OO468_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR6258270.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39541..39948
                     /locus_tag="OO468_001071"
     CDS             39541..39948
                     /locus_tag="OO468_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR6258271.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(39988..40509)
                     /gene="fldB"
                     /locus_tag="OO468_001072"
     CDS             complement(39988..40509)
                     /gene="fldB"
                     /locus_tag="OO468_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR6258272.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40621..41517
                     /gene="xerD"
                     /locus_tag="OO468_001073"
     CDS             40621..41517
                     /gene="xerD"
                     /locus_tag="OO468_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR6258273.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41542..42252
                     /gene="dsbC"
                     /locus_tag="OO468_001074"
     CDS             41542..42252
                     /gene="dsbC"
                     /locus_tag="OO468_001074"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR6258274.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42258..43991
                     /gene="recJ"
                     /locus_tag="OO468_001075"
     CDS             42258..43991
                     /gene="recJ"
                     /locus_tag="OO468_001075"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR6258275.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44082..45180
                     /gene="prfB"
                     /locus_tag="OO468_001076"
     CDS             join(44082..44156,44158..45180)
                     /gene="prfB"
                     /locus_tag="OO468_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR6258276.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45190..46707
                     /gene="lysS"
                     /locus_tag="OO468_001077"
     CDS             45190..46707
                     /gene="lysS"
                     /locus_tag="OO468_001077"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR6258277.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(46750..47298)
                     /gene="idi"
                     /locus_tag="OO468_001078"
     CDS             complement(46750..47298)
                     /gene="idi"
                     /locus_tag="OO468_001078"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR6258278.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47353..47424
                     /locus_tag="OO468_001079"
     CDS             47353..47424
                     /locus_tag="OO468_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258279.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47421..47546)
                     /locus_tag="OO468_001080"
     CDS             complement(47421..47546)
                     /locus_tag="OO468_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258280.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47548..48996)
                     /gene="uacT"
                     /locus_tag="OO468_001081"
     CDS             complement(47548..48996)
                     /gene="uacT"
                     /locus_tag="OO468_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR6258281.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11864 a        12329 c        13259 g        11731 t
ORIGIN      
        1 tgctgcggcg tcgctggcag agcctatgtg ctgcattatt ggtgcttatc atgccaatta
       61 tcacaccacg caatatgttt atgagcatcg catgggcgtc aaacctggcg gcaatattgc
      121 actgctggcg tgtgcgggtc cgatgggcat tggcgctatc gattacgcca ttaacggtgg
      181 catacaaccg tcacgggtgg tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca
      241 gaagctgctg ccggtggatc tggcggccag taaaggcatt gagctggtgt atgtgaatac
      301 caaagggatg agcgatccta tccagatgct gcgggcgctg acaggagatg tcgggttcga
      361 tgacattttt gtttatgcgg cggtgcctgc tgtcgttgag atggctgacg aattactggc
      421 ggaagatggc tgtctgaact tctttgccgg gctgacggat aaaaacttca aagtgccgtt
      481 taatttctac aacgtccatt acaacagcac gcacgtagtc ggtacatccg gcggttcaac
      541 ggacgacatg aaagaggcga ttgcccttag cgccacagga cagttacagc cgtcgtttat
      601 ggtgacccat attggtggcc tggatgcggt gccagaaacc gtgctcaatc tgccggatat
      661 ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctcactg ccattgccga
      721 ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ttggcgcggc tggttgagga
      781 aacgcacggc atctggaatg aacaggccga gaaatatctg ctggcacaat ttggcgttga
      841 tatcggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt tacggaacag
      901 gcagcgttgg ctgcctggcc gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg
      961 gcggtcaccg cgatgcgcca ggcattaaac gacgtcgctt ttcgtggacg agtggttatc
     1021 ggcgaaggag aaattgacca tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc
     1081 gatgggccag aagtcgatat cgcggttgat cccattgaag gcacgcggat ggtggcgatg
     1141 gggcagagca atgcgctggc ggtcatggct ttcgctcctc gagatagcct gttacatgcg
     1201 cccgatatgt atatgaaaaa gctggttgtt aatcgactgg ctgccggggc gattgatctg
     1261 tctttgcctc tggcggacaa cttgcgtaac gtggcgcggg cgttaggtaa accgctggac
     1321 aagctgcgca tggttacgct ggataaaccg cgtctgagtg ccgcgattga agaagcaaca
     1381 cagctgggcg tgaaggtttt tgccctgccg gatggcgatg tcgccgccag cgtgctgacc
     1441 tgctggcagg ataatcctta cgatgtgatg tacaccatcg gcggtgcgcc agaaggcgtg
     1501 atttctgcct gtgccgttaa agcgttaggc ggggatatgc aggccgagct gattgacttt
     1561 tgtcaggcta aaggggatta cacggaaaat cggcagattg cagagcagga gcgcaagcgt
     1621 tgtaaggcaa tgggcgttga cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg
     1681 aacgatatcc tctttagcgc tacgggcgtg acgggcggcg agttggtgaa cggtatccaa
     1741 cagacggcga atggggtgcg gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt
     1801 aatataatag actccctgca ttgatggtga tctatcggaa gaccaatggc gacgctgaca
     1861 gaagatgatg tgcttgagca actggatgca caggacaatt tattttcatt tatgaaaact
     1921 gcgcattcta ttttgctcca ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac
     1981 gatgaagaga tcgtcgaata tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac
     2041 gatattgatg ttgcgctgcg tttgatttat gcgctgggga aaatggataa atggctgtat
     2101 gccgatatca cgcacttttc ccagtactgg cattacctga acgaacagga tgaaacgccc
     2161 ggatttgccg atgatataac ctgggatttt atcagcaatg tcaacagtat aactcgcaat
     2221 gcgacgctct acgatgcgtt aaaagcgatg aagttcgccg atttcgccgt ctggtcagag
     2281 gcgcgtttta gcggaatggt caaaacggcg ctgacgctgg cagtaacgac caccttaaag
     2341 gaattaacgc cgtgaaaatt gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc
     2401 atgatgaaga gatagaacgc gtgcataaac tgttgctgcg tatgctggcg gccttacaga
     2461 cggtaaatcc gcagcggcgt acggtggttt ttctttgcgc tccgccgggg acgggtaaat
     2521 ctaccctaac caccttctgg gaatatctcg cgcaacaaga cccggagctg cccgctattc
     2581 aggcgctccc gatggatggt tttcaccatt acaatagctg gctggacgcg catcaattgc
     2641 gccccttcaa aggcgcacca gagacatttg acgttgcgaa actggcggaa aatctgcgcc
     2701 aggtggtgga aggggattgt acgtggccgc agtacgatcg acaaaagcat gatcctgttg
     2761 aagatgcgtt gcacgttacc gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg
     2821 acgacgagaa gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc
     2881 cagctgctgc gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg gtatcgctgg
     2941 cagatgcaga agccttttat gaccgtaccg acgggccgaa tgttcgccgg gggctggaag
     3001 agagtctgcc tgcaaatttg accttaatga tgaccgctac gggtgaatat cgtctggtgg
     3061 attaacgttc ctgattacac catcttccag ccgcaccaca cgagagaaat gacgccgggt
     3121 aaatgcggcg tcatgactga ttgctaccac gctcgtgcct cgctggcggc acttctccag
     3181 ccagctttca aacacgctca accaattttc gtcaaaatcc cgactcggct cgtccagcag
     3241 taatagtggc ggagagagtg cttccaggca ggcgacggcg accatccgtc gctgcgcatt
     3301 atgcagatcc aacgggtgcg agttggcagt gtctgctaac tgacaacatt gcagggcagc
     3361 atgggtacgt tgagtgattt catccgccgg gcatttttgc agtttcaaac caaatgcgat
     3421 ctcgtcagcg acggtgctgt gaaaaagttg gttttccgct tcctgaaaca gtacgccaac
     3481 ctttgccgcg cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt
     3541 accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt tccctgcgcc
     3601 attgtcaccg gtcagcgcca accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag
     3661 acaatccgtt gccgcgccag gccagcgata agatatctta tttaacgtta acatagtggc
     3721 tggagcgctc cgttttgcaa ttgccacgct tgctggcagt agttcaggaa aggtgtgcga
     3781 tgacgttcaa aaagaataat cagcgaacca cgttcaaacg cccagtgttg taagcgctgc
     3841 aacagcattt cgctggcctg cggtgtcagg cggctaaagg cttcatcaag aattaagagc
     3901 tttggctgca tcgcgatggc gcaggcgatg atcacgcgct gtgtctctcc accggagagt
     3961 gttgccgggt gacgatggcg caacggctgg cactcggtca gagtcagggc ggcatcaatc
     4021 cgcgccataa tctctgcttc agcaagacac agattttctg gcccaaaggc aacttcttct
     4081 tccacgctaa aggtacagcc ggagagctgc aaatagggcg attgctggac gagctggatg
     4141 gtggcggatt gttcattaag cgggagatgc ccaatgggcg taccgaggag cgtccccgtg
     4201 cctgttatct ctcttggcag aaagtccgga taccagcccg ccatcagctg tgccagcgta
     4261 cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac atagtgaaag
     4321 tcataacaga acggaggatg cgttgagtgt gtcgggcaat aacggaactg ttctaacgta
     4381 accatatcca cgctccgaat tctgtcagca tcagcaagat catggtgtaa cgcgctacgc
     4441 gttgtagggt gctatcggct ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg
     4501 cccgcatatc cagcgccgca ccacggacag ccagatcgtt aagcgcattg tgtgttagcg
     4561 gaataattaa cgcgggcata gcacgtaaac gctgatacca gccctcatcc agcggcacgc
     4621 cgcgggcacg ctgcgcttca tgaataatcg ctaattgccg ttttaactgc tcaacgacca
     4681 ataacggccc ggcaaacaga taagcgacgc ctggcggcag gcgagaagca aacagagcgc
     4741 gaataaaaca ttgcacgggg acgtactgca ttcacaactg tgaggttgaa acaatcgcca
     4801 gaatcagcaa ccagagcgta atagcatgcg accaccgttc tggcgagcgt ggcgtgccgc
     4861 ttagccattc tggtagccag ccaccgtgta ccagccacag cccggctccc agcgagaaca
     4921 tcagccagac gacatatttt gcccgccgcc gcgtggcctt tagcgcaata aggcagaaaa
     4981 aggtcgccgc gctatagatc ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg
     5041 ccgctaatgc ccacagtgtt aatgaggtaa atggatgcat ttagcgcacg gcagccattg
     5101 ccggaaaatg gcgcgtggtg cgaatcggca gctggcgcag taaaagccag acaatcaccg
     5161 ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc gactccacca
     5221 ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgcta ccggtgacac
     5281 cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc gtaataatca
     5341 cgccgctgac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg
     5401 agaccaggcc gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg tcggtcagca
     5461 atccccagag taaattggtc agtaagccgg tcagcattcc gatcaccgga ccaagcagta
     5521 ccgcgctaat aagtgtgccg atggagtcga gaaatatagg taatttgacc atgctggcca
     5581 gttgcccgcc gatcatattg atggcgatag atataacgat caacaccagt gcctgactgg
     5641 aaaagtggct acgcgccata agaaaacctc tttgaaagta gttatttaac gggggaaata
     5701 tgcaactcgt cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg
     5761 ctaccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat atgaccgcca
     5821 agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gctcaccact ctgttccagc
     5881 gtgccgttta gtgagatcac ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg
     5941 gcatagcgca aggcgacgtc agtcaggcta ccggtacagc ctgcaatcta ggcggcatga
     6001 agttgatgtt gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg
     6061 cgcagtgcgt aaaagcgtgc ggaggaagaa tatgagtagg gcaatgtcat cattggagta
     6121 ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata ttctgatata
     6181 agtcagggtt tgtgacggca caacgaaaag attttgctac gcttattcgt gattctgcaa
     6241 agtggaataa attccacagg ggcggggctg aaaacgtgac gtaagtcaga taattgcttt
     6301 ctttgactgg acaatcattc cttttattcc acgtttcgct tatcctagct gaagcgtttc
     6361 agtcgattaa atgttcgaca attaaccaat cagtcgcagt ttgcgacagg taaggtttcc
     6421 ccggacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac cttgcaggag
     6481 atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta atgtggttcg
     6541 tgctttgtat gaatccggac gccgggcgga aattaccgtg gtggcaatca acgaactggc
     6601 ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc gttttgcatg
     6661 ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc gcgtattgca
     6721 tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag tcctcgactg
     6781 caccggcgta tatggctccc gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa
     6841 agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt acggcgtcaa
     6901 tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta ccacgaattg
     6961 cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtccg gcactgtgac
     7021 cacaattcac tccgccatgc acgatcaaca ggttattgat gcataccatc ctgacctgcg
     7081 tcgcacccgg gcagccagcc agtcgatcat tccggtcgat actaaactgg ccgctggtat
     7141 cacacgattt tttccgcaat ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac
     7201 cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa aagccaatga
     7261 agtcaacctg ttgctgcaaa aagcagcaca aggtgcattt catggtatag ttgactatac
     7321 ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca ttgtcgatgg
     7381 cacccaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt gcgataacga
     7441 atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg ttgctttcag
     7501 gtaagacgca agcagcgtct gcaaaacttt tagaatcaac gagaggattc accatgtctg
     7561 taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt gcggatctga
     7621 acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct tctctgccga
     7681 ccatcgaact ggccctgaaa caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc
     7741 ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac tacctgaaag
     7801 acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt gacgttgctg
     7861 aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg
     7921 aaaccctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac gcattcggta
     7981 ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgac gttgcgtgcg
     8041 caggcccgct gctggcagct gaactggacg cgctgggtaa agcactgaaa gaacctgctc
     8101 gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc gttctggact
     8161 ccctgtctaa aatcgctgac cagctgattg ttggtggtgg tatcgctaac acctttatcg
     8221 cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta
     8281 aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt
     8341 tctctgaaac tgcaccggct accctgaaat ctgttaacga tgtgaaagct gacgagcaga
     8401 tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag aatgcgaaaa
     8461 ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc aaaggtactg
     8521 aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc ggcggcgaca
     8581 ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc tccactggcg
     8641 gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg atgctcgaag
     8701 agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcacttgttt cagcgcgctt
     8761 ttagagcacg cattttttca tactctaaat aattcgagtt gcaggaaggc gacaagagag
     8821 tgaatcccca ggagcttaca taagtaagtg actggggtga gcgaacgcag acgcagcaca
     8881 tgcaacttga agtattatga gtataaggcc cgacgataca ggacaagaga catgtctaag
     8941 atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa agttttccag
     9001 gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac tgactccatc
     9061 aacgccgtac tggaaactgc tgctaaagtt aaagcgccgg ttatcgttca gttctccaac
     9121 ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca gggtgctgct
     9181 atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt
     9241 ccggttatcc tgcacactga ccactgcgcg aagaaactgc tgccgtggat cgacggtctg
     9301 ctggacgcgg gtgaaaaaca cttcgcagct accggtaagc cgctgttctc ttctcacatg
     9361 atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa atacctggag
     9421 cgtatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg tggtgaagaa
     9481 gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc ggaagacgtt
     9541 gattacgcat acaccgagct gagcaaaatc agcccgcgtt tcaccatcgc tgcgtccttc
     9601 ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac catcctgcgt
     9661 gattctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct gaacttcgta
     9721 ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag ctacggcgta
     9781 gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt tctgaactac
     9841 tacaaagcga acgaagctta tctgcagggt cagctgggta acccgaaagg cgaagatcag
     9901 ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc
     9961 gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta agtcattcct
    10021 ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca aggtgattca
    10081 gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact
    10141 gcccgtcata aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg
    10201 aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa
    10261 ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt
    10321 tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa
    10381 aatcgatgcc actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt
    10441 tacgctaatc gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact
    10501 cggtgccgca ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc
    10561 tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg
    10621 cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg
    10681 taaaattatc gttattccga acggtaaaat tattgccgga aatattatta acttctcccg
    10741 cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca
    10801 ggttaagcag atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga
    10861 aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg
    10921 gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga
    10981 atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt
    11041 gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc
    11101 ttattagtta ttctgattgc caattaatat tatcaatttc cgctaataac aatcccgcga
    11161 tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca
    11221 aggtcttgct cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat
    11281 gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga
    11341 tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg
    11401 gttgctagcg ctggtcacct ggggcggcgt agtcttcttg ctctggtatg gttttggcgc
    11461 ttttaaaaca gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg
    11521 cagatggaaa attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct
    11581 ggatactttt gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg
    11641 gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg ctattctcgc
    11701 agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt
    11761 gggatgtgtc atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca
    11821 agccttgttc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct
    11881 ccagaggtcc tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa
    11941 gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg
    12001 ccggatggac cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt
    12061 gccactcttg cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag
    12121 gcagatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa
    12181 gatatcagct cagcgaattt acgcacccag ccagattatg actatcagga tggtaaaagt
    12241 atcctgaaag gctaccgagc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa
    12301 ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttctgtgtcg
    12361 ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac
    12421 gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat
    12481 agcgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc
    12541 gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat
    12601 caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca
    12661 gcacaataat acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc
    12721 tcctttttct gtaattttct gtaattttct gtaaaaccat gacgtaatca cctatctttg
    12781 ctcgcggttt catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggag
    12841 gggggatgga catttttatt tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca
    12901 ataatattgc ccgggcggca gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca
    12961 ttgccgcgct ggaagaacaa attggctata cgctatttac ccgcaaagat aataatatca
    13021 gcctcaataa agccggtcag gaactgtatc aaaaactgtt cccggtttat caacgacttt
    13081 ctgcgattga taatgaaatc cataactcag ggcgtcgttc acgagaaatt gtgattggca
    13141 tagataatac ttatccgaca attattttcg atcagttgat tagtctcgga gataagtatg
    13201 aaggcgtgac cgcccagcca gttgagttca gcgaaaacgg cgttatcgat aatctttttg
    13261 accgtcagtt agattttatt atctccccac agcatgtatc tgcacgagtt caggaactgg
    13321 aaaacctgac tatcagtgaa ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt
    13381 atgaagagcg tcaagagcaa gagctgcttc aggagctgcc ctggctacaa atgcgctttc
    13441 aaaaccgggc taacttcgaa gcaatgattg atgcgaatat gcgaccttgt gggatcaatc
    13501 ccaccattat ttatcgtccc tacagtttta tggccaaaat cagcgccgtg gagcgcggac
    13561 attttctgac ggtgatcccg cattttgcct ggcgactggt gaatccggca acgctgaaat
    13621 attttgatgc gccgcacagg ccgatgtata tgcaggaata tctttattca atcagaaatc
    13681 atcgttatac cgccacgatc ttcagcatat tgctgaagat cgtgacggga caaaccatta
    13741 acccagcatc gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc
    13801 gtggtgaata tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat
    13861 aggatgtgca caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag
    13921 atcggcgatc ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg
    13981 gctgcacatt ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa
    14041 gatcgacaga tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc
    14101 gaccccggct acgtgcgttg aatttgcatg cccgtaaata tcaaactcca ggccgacgtt
    14161 cagagcgatg acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg
    14221 caacacaatg cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc
    14281 cgagattgtc aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac
    14341 caccgattcc tgtagcactt ccgaatacat catgaacgga ggaatttccg ggttttcccc
    14401 cagacgtgcc attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc
    14461 cggcggaata cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat
    14521 ctgctggcac atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc
    14581 cacgacggcg acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat
    14641 tgcatggaag atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatctgc
    14701 cagttctgca acgcgcggat cgtgatagtg attaagttca atgatcactt tcttcgctcg
    14761 cagcagctag gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc
    14821 cagtgccgat gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat
    14881 ttgcgccact tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt
    14941 acgtaaactg gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag
    15001 tacatcgtca gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt
    15061 tttggcctca tgctgttcgt tagctctgcg ggcaatcgcg gcgggtaggg ctttcggcga
    15121 acccgctggg gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc
    15181 ttcatcggcg gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat
    15241 taatgaccaa cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa
    15301 tcttcgctgt cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc
    15361 atggtgtgtg cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc
    15421 gctttctcag agatgtggtg cgccatttgt aaggtgaaat cttccagttc ttccacttcc
    15481 acaacatggt tgaggatgcc gacagccagc gcgcgctggg cggtgattgg cgaagcggta
    15541 aaaatcagct ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg aatgccgacc
    15601 aggttatacg ggacgccgag gtttacaggc gtcattgaga aggttgaggt actggcggcg
    15661 atgatcagat cggaactcat gatcatttca aatgcgccac cccaaacact accttccacc
    15721 atcgaaatga tcggtttcgg gaatttttgg atcatgcggg tgatttgacg caatggatca
    15781 tcataggaga gcggatcgcg accgccagac ggcagttcgt gaatatcgtg acctgcggag
    15841 aagactttgg atccacttgg cgcccgcaaa atgatacagc gaatttccgg ccggttgaga
    15901 tcgcttaacg cctgcataag atcatcaata aagactttac ttaaggcatt aagttttcgt
    15961 ccatagttaa actcaatgac tgccactttg ttgatagtgg caacgttcac atactgataa
    16021 gacataaaaa ttcctttaat caaaatattg cgtctggata aattcactga gctgccgcag
    16081 gccggtgcgc ggtgagagcg tattgttttt gaccgctaaa agcgtctggc ggtaatagcg
    16141 atcgaaatct tcattcgcga acaggtgatt cagtacttct tcttcggtct gcttacgcag
    16201 ccattccacc gattgttgtt gccgcacttg ttgtaaacga ccactggcag ttagcgcggt
    16261 tttgaagtcg atgatggcgt gccagatctc atcgattcca cgtttttcca gtgcgctaca
    16321 agtcagaccc cgtggctgcc attcgtcgta tttacgtcgc agaatatgca gggcactctc
    16381 gtacatatgc cgggcaatgg cgacattggt atggttatcg ccatcgtctt tgttgataac
    16441 gagcagatca gccacttcca tcagcccttt tttaatgccc tgcagatcat cgccgccacc
    16501 ggcaatttgc aacgagataa aacagtccac catgcgggcg acttccgttt ccgactgcgc
    16561 gatgccaacc gtttcgacaa tcactacgtc ataacccgcc gcttcgcata acagcattaa
    16621 ttcccgtgct cgctgactgg caccgcccag atgaccggag gagggtaccg ggcgaataaa
    16681 cgccgcctcg gcacgcgcca ggtcattcat gcgggtttta tccccgagaa tgctaccgcc
    16741 agtgaccggg ctgctgggat cgaccgcaat aacagcaacc tttaatccct cacgaatcaa
    16801 caacatgcca aaggcttcaa gaaaggtact tttccccgcg ccgggtgtgc cggtaacgcc
    16861 cagtcgcagg gcgttaccac agaacggcat aatagcgtca agcagctgtg tacttagcgc
    16921 ctgatgacgt gggtgacggc tttccaccag cgtcatggct tgggcgagcg tggcacgctc
    16981 accctgacgt aagcggcgaa tactttctgc cagcgtggct tcattaatca tgatgctggc
    17041 ttatcagatt cagtacgtcg cgcacactgt cgagcatagg tgtacctgga ccataaatcg
    17101 ccgccacgcc gcgctcttgc aggaaggcgt aatcctgcgg cggaatgacg ccacccgcga
    17161 ccacgcagat atcttcgcgt ccccattttt tcagcgcttc gaccagttcc gggatcagcg
    17221 ttttatgacc ggcagccagt gaggatgcgc ccactacgtg aacgtcgttt tctacggcta
    17281 ggcgggcgat ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg aaaccgagat
    17341 cggaataggc gctggcgatc actttcgcgc cgcgatcgtg tccatcctgg cccatcttag
    17401 cgatcagaat gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt tgcgcaacaa
    17461 tggcatcgaa ctcggaggcc gatttctcag actgatgata gctttgcgca atcacgccgg
    17521 taacacactg gcttggcacc agataacggt cgaaagcgac ttccagcgca tcggaaattt
    17581 cacccagggt ggcgcgaacg cgagcggcat taacagcggc agccagcagg ttttcgttat
    17641 gctgtgcggc gtgagtcagg gcgttcaacg cggcggttac ggcggcatca tcacgggtgg
    17701 cgcgaatgcg ttccagcgaa gcaatttgct cgttacgcac catcacgttg tcgatctcaa
    17761 gtacatcggt ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg atgacacgct
    17821 tgccctggtc gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt cgttttggca
    17881 gacctgcttc gatcgctttc gccatgccac cggcttcgtc gatctgttgg ataatagctc
    17941 tggcttgttt gacgatttga tcggtcagcg actcaatgta ataggatccg gccagtggat
    18001 cgacggtgtg gcagagttct gattcttcct ggatgatgat ctgggtgttg cgggcaatgc
    18061 gtgctgagaa atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta tgcagtgact
    18121 gagtaccgcc cagcgtcgca gccagtgctt caatggtggt gcggataacg ttgttatacg
    18181 gatcctgttc agtcaggctc cagcctgagg tctggcagtg ggtacgcagc gccagtgatt
    18241 tcggatcctg tgcgccaaat ccactgaccg cttcgctcca taaataacgt gccgcacgca
    18301 acatggcgac gttcataaac agatccatgc cgataccgaa gaataacgac aggcgaggag
    18361 cgaagtcatc aattttcagt ccggcagaga ttgctgcttt gatgtactca atcccatcgg
    18421 cgagcgtaaa tgctacctgc tgcacgcagt tggcacccgc ttcccccatg tggtaaccgc
    18481 tgatactgat ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg atgtcggcga
    18541 taatgcgcat tgacggtttt ggtgggtaaa tataggtgtt gcggcagagg tactctttga
    18601 gaatatcgtt ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc tcttctgcgg
    18661 cgacgatata aaacgccagt actggtagca ctgcgccatt catggtcatc gaaaccgaca
    18721 ttttatccag cgggatctgg tcgaacagga ctttcatatc ttccacggtg tcgatagcga
    18781 cgcccgcttt gccgacgtca cccgccacgc gtgggttatc ggagtcgtag ccacggtggg
    18841 tggcaaggtc aaacgcaacg gaaagacctt tttgcccggc ggccaggtta cggcgataaa
    18901 aagcgttgga ctcttttgct gttgaaaaac cagcatactg acggatggtc cacggttggg
    18961 cggtatacat agtggcacgc gggccacgaa cgtagggcgg caaaccagga agggtacctg
    19021 tcacctccag attatcgaga tcggcttcgg tatacagcgg cttgatggcg atcccttccg
    19081 cggtttgctg aaccagcgag tcgacagttt tctcccgacg gctcaattcc ttgttggcaa
    19141 gctgttgcca ctcctgctcg ttagacatta tttgctccgt aaaacgcaat caaaatgagg
    19201 gtatttgcgc ttacggtgag ctttttacgt gggtttgtcg ccagcaaaaa aaatgcattc
    19261 agacgtcgtt ttttgccgcc tgagatttgt ctattttgtg atgtgattca aggaatacaa
    19321 aaagaagtga ggacaacgcc tgatatgtgc tatcaggcat tatttgatgg attaatcctg
    19381 acgaaggact ttgtgaccat aatcgagtaa cgcatcagtg actttacgca tcatgcggct
    19441 ttcaggagca aagcggtgcc agtagagcat ccgtcgttga aatagcccag gcgttaagtc
    19501 aatcagttca ccgctggcca gctctttctc gatttgcagg tgcgggatca tacagcaggt
    19561 ggtgccctgg cgagcaagtt gtacgaacgc ttctgaagaa ttaacgatat ggcagggtac
    19621 gctgccagga ggaagatcga agttttgctg caaaaaggcc tggtgcatat cgtcaagatg
    19681 gtcaaacgcg accactggcg ctttcaataa tgccgaacgc gttacgccgt taggaaaata
    19741 tttttcggca aacggttttg agctgacgaa cagatagtcg agcgcaccga gtttatcgac
    19801 aagacaactc ggcagcgcct gatgttgaat actcaccgcg ccgaccactt cgccgcggcg
    19861 cagacgttcc tgagtacggg tttcatcttc tacctgcaag ttgaggcgga taggcgaatc
    19921 agccaacaca ggagccagtg ccggaagcaa ccacgtcgcc agactgtcgg cgttgaccgc
    19981 cagtgaaagc agcagcggag tcgaaccggt ttgttcatcg cccagccact cttcttccag
    20041 caactccacc tggcgcagca gtgccagcag tttttgccct tgttccgtcg ggcgcggcgg
    20101 tacggtacgc accaacagcg gctgcccgaa catattttcc agttgcttaa tgcgctgtga
    20161 gacggctgat tgtgtaatgc acagcttttg tgccgcgcgc tcaaatcctc gttcacgtat
    20221 caccgcatcc agtgcctgta atgttctgta gtccgggcgt ttcattgctg ttgtctgctc
    20281 ctggtaaatg ctttttcctg cactatgaca caattttgtg tcaggttgca tataccgaag
    20341 ccgtcggcgg gtaacatttg tatcaatggt taaacgcgca gcttaaaaga tcagggattt
    20401 gcgctattgc atttcgctta gcgacttcgc gccctggtta tgttgaatgg cgtggcgtta
    20461 ttgcctctgt tcgcaccgtg gagcggcata cccagaccgt tgtatgcttt atttgcgggg
    20521 cgaaagggga tgcccgccat tgcgcgatat tttatggatg agttaaccac ccggcttgcc
    20581 aacggggtct gaatcgcttt ttttgtatat aatgcgtgtg aaatttcata ccacaggcga
    20641 aacgatcatg acgcaggatg aattgaaaaa agcagtagga tgggcggcac ttcagtatgt
    20701 tcagcccggt accattgttg gtgtaggtac aggttccacc gccgcacact ttattgacgc
    20761 gctcggcacg atgaaaggcc agattgaagg ggccgtttcc agttcagatg cttccactga
    20821 aaaactgaaa agcctcggca ttcacgtttt tgatctcaac gaagtcgaca gccttggcat
    20881 ctacgttgat ggcgcagatg aaatcaacgg ccacatgcaa atgatcaaag gcggcggcgc
    20941 ggcgctgacc cgtgaaaaaa tcattgcttc ggttgcggaa aaatttatct gtattgccga
    21001 cgcctccaag caggttgata ttctgggtaa attcccgctg ccagtagaag ttatcccgat
    21061 ggcacgtagt gcagtggcgc gccagctggt gaaactgggc ggtcgtccgg aataccgtca
    21121 gggcgtggtg accgataatg gcaacgtgat cctcgacgtc cacggcatgg aaatccttga
    21181 cccgatagcg atggaaaacg ccataaatgc gattcctggc gtggtgactg ttggcttgtt
    21241 tgctaaccgt ggcgcggacg ttgcgctgat tggcacacct gacggtgtca aaaccattgt
    21301 gaaatgatct gacgggggaa cctcccccgt taaaaaaatt ctcttcatta aatttggtga
    21361 catgtgtcac gcttttacca ggcaattgtc gattgctcta aataaatcct ctaaaccagc
    21421 atattcatcc aagaattacc tttgcgtgat atttcctcaa catcgcgacg caaacgttca
    21481 tattgccgca atattatttt ttgatatgtt gaaaggcgga tgcaaatccg cacacaacat
    21541 ttcaaaagac aggattgggt aaatggcaaa ggtatcgctg gagaaagaca agattaagtt
    21601 tctgctggta gaaggcgtgc accaaaaggc gctggaaagc cttcgtgcag ctggttacac
    21661 caacatcgaa tttcacaaag gcgcgctgga tgatgaacaa ttaaaagaat ccatccgcga
    21721 tgcccacttc atcggcctgc gatcccgtac ccatctgact gaagacgtga tcaacgccgc
    21781 agaaaaactg gtcgctattg gctgtttctg tatcggaaca aaccaggttg atctggatgc
    21841 ggcggcaaag cgcgggatcc cggtatttaa cgcaccgttc tcaaatacgc gctctgttgc
    21901 ggagctggtg attggcgaac tgctgctgct attgcgcggc gtgccggaag ccaatgctaa
    21961 agcgcaccgt ggcgtgtgga acaaactggc ggcgggttct tttgaagcgc gcggcaaaaa
    22021 gctgggtatc atcggctacg gtcatattgg tacgcaattg ggcattctgg ctgaatcgct
    22081 gggaatgtat gtttactttt atgatattga aaacaaactg ccgctgggca acgccactca
    22141 ggtacagcat ctttctgacc tgctgaatat gagcgatgtg gtgagtctgc atgtaccaga
    22201 gaatccgtcc accaaaaata tgatgggcgc gaaagagatt tcgctaatga agcccggctc
    22261 gctgctgatt aatgcctcgc gcggtactgt ggtggatatt ccggcgctgt gtgatgcgct
    22321 ggcgagcaaa catctggcgg gggcggcaat cgacgtattc ccgacggaac cggcgaccaa
    22381 tagcgatcca tttacctctc cactgtgtga attcgacaac gtccttctga cgccacacat
    22441 tggcggttcg actcaggaag cgcaggagaa tatcggcttg gaagttgcgg gtaaattgat
    22501 caagtattct gacaatggct caacgctctc tgcggtgaac ttcccggaag tctcgctgcc
    22561 actgcatggt ggtcgtcgtc tgatgcacat ccacgaaaac cgtccgggcg tgctaactgc
    22621 gctgaacaaa atcttcgccg agcagggcgt caacatcgcc gcgcaatatc tacaaacttc
    22681 cgcccagatg ggttatgtag ttattgatat tgaagctgac gaagacgttg ccgaaaaagc
    22741 gctgcaggca atgaaagcta ttccgggtac cattcgcgcc cgtctgctgt actaattccc
    22801 cttctctgaa aattaacggg caggtcactg acttgcccgt ttttttatcc cttctccaca
    22861 ccgttgccgc gttgcgcatt ctactttgcg agtctgctcc agaaaataaa tcaaagtcag
    22921 atcaatgcgt tgtgtttatt taatggggat ttacgatggc gggacagcat ggggctgtaa
    22981 cggtaaagcc ctcaccgaag cgagggctta aaggagaagg gttatgatgc gacttgtcat
    23041 catactgatt gtactgttac tgataagttt cggagcttat taacagtcag tctcaaggga
    23101 ggagtcatcc tcccttaccc ttactcacta aattaggtca aagaatcaac gatgtcaatc
    23161 agggcgatgc ggggtgcatc gccctgaacc gaactttttg atagccactt catcgcccac
    23221 gtttactgcc gtaatagatc ctttttccca aaaattatct ctgttttgca tggggttttt
    23281 cgagcacgct cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga
    23341 gcttacgata gctgggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct
    23401 tgaaggagaa gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt
    23461 ttcccagctt attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac
    23521 taaattaggt taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc
    23581 actcccagac tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg
    23641 ggagtttttc caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct
    23701 gccagttttg taaggtccga tcataaaaac caccgcccat tcccagacgc tgaccgtact
    23761 catcaaaggc gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac
    23821 gcacatccag ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat
    23881 ggtaattcag gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc
    23941 gcttaccggc gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag
    24001 agaggaatac agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag
    24061 cggcttgttg acccatttcc agctgttgtt ccggcgttaa cgcacgacga cgttgccgaa
    24121 tcattttgcg gatttcttgt cgggataatg tcagtgggag ctctggtagt tgcgtcatgg
    24181 ctggtgccag ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc
    24241 ttggttcaag gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacgaac cgagcatgct
    24301 caccaaccgc ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg
    24361 cttgcgaaca tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa
    24421 gtgtcattca aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg
    24481 ttctatggtc tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt
    24541 ctttgctttt tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg
    24601 ttcagtattt gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc
    24661 cgctgcctga ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc
    24721 aaaaatttgg atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct
    24781 gccttcgtct ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa
    24841 gagacaagcc cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct
    24901 ccctttgacg acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga
    24961 accagtatct gaaccaacaa gggacgggtc tgacccctgc cgagatgcat ggtttaatca
    25021 gtgggatgat atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga
    25081 cgaacgaagg catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg
    25141 ccaccagcga tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg
    25201 atgatgtcag cgttttcgat cgggctgatg cactggcagg ttgggtcaat cacttcctgc
    25261 ttggtcttgg cgttacgcaa ccgaagctgg acaaagtgac cggcgaaacc ggtgaagcca
    25321 tcgacgatct gcgtaacatc gcgcagctgg gctacgacga agacgaagat caggaagagc
    25381 ttgaaatgtc gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg
    25441 acacctttac tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa
    25501 aacaaaaaac gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc
    25561 cgtcaggccc tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca
    25621 gaagtaacac gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac
    25681 ttcaccggct ttaacgaacc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat
    25741 aaccacagcg ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt
    25801 cgcttaggcc aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc
    25861 gaaatcaatc agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag
    25921 ggcgaatatg catatgctga tgaaatcgtg aacagtgcgc tggaaaaact gcgtaaaggt
    25981 tcgcggcaaa atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa
    26041 atgcgcctgt tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc
    26101 gccatggcac atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg
    26161 gaaggcgaaa ttcaccacga atttaaccgc cacggtgcgc gctatccttc ctataacacc
    26221 attgtaggca gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg
    26281 cgcgacggtg acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat
    26341 attacccgca ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac
    26401 attgtgctgg agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg
    26461 gaagtcactg gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg
    26521 aaaggtaatg ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc
    26581 cttagccact ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg
    26641 cgcattctgg aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat
    26701 gcggatgtgc cagaacaata tcgcggtatc ggcattcgta ttgaagacga cattgtgatt
    26761 accgaaaccg gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa
    26821 gcgttgatgg ctgctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg
    26881 gcgcgacgct ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga
    26941 ttgaagcgac tgcgccagag tcacatgctc atccgggctt tgatggacga gcgatagcgc
    27001 tggcggcggg tacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggctgatt
    27061 gcgcaaccgc catcactacc gtgcatgtca gcgatcgtgg tcatgctgga ttcgtcaccc
    27121 tcgccgcaga agattaccaa ctggcggcgc tggggcaggt tgtcgaattg cataatgtcg
    27181 ggcaacggct gtttgcattg ctgcgaaaag cacctggcgt aatgctgcat tgccccgatc
    27241 gcgtagctaa cgttgcccgt actcagagtc atgttgaagt gacgctggag agcggcgaga
    27301 tgctgacggg ccgcgtgctg atagccgctg atggcaccca ttcagcgtta gccaccgcgt
    27361 gcggcgttga ttggcagcag gagccttacg aacaactggc agtgattgcc aacgttgcta
    27421 cttctgttgc gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga
    27481 tgttgccgat gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg
    27541 aagaggtgct gtcgtggagc gacgagaagt tttgccgtga actccagtct gcctttggct
    27601 ggcgactggg gcaaattacc cacgccggta aacgcagtgc ttatccgctg gcattaaccc
    27661 gcgccgccag accgattacc catcgcactg tgctggtggg caatgcggcg caaactctgc
    27721 acccgattgc cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa
    27781 ccctgactca ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct
    27841 atcagcagcg tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac
    27901 atctttttgc caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga
    27961 tggaattatt caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc
    28021 gttgaaggcg gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggcggc
    28081 ggcatggtgg ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta
    28141 ctggagcagc gcgtaccgga acctctggcg gtagatgcac taccacaact gcgcgtttcg
    28201 gctatcaatg ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc
    28261 gctcgtaggg ccagttgtta tcacggcatg gaagtgtggg acaaagacag ttttggtcac
    28321 atctcgtttg acgatcaaag catgggctat agccatcttg ggcacatcgt tgaaaactca
    28381 gtgattcact acgcgctgtg gaacaaagcg cagcagtcgt cagatattac tctgttggcc
    28441 cccacagaat tacagcaggt cgcctgggga gaaaatgaaa ccttcctgac gctgaaagat
    28501 ggcagcatgt taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc
    28561 aacaaagcag atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc
    28621 attcgcacgg aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt
    28681 ctggcctttt taccgcttag cgatccgcat ctttgctcga ttgtctggtc actgtcgcca
    28741 gaggaagcgc agcggatgca gcaggcaagt gaagacgaat ttaatcgcgc gttaaatatc
    28801 gcttttgata atcgccttgg cttatgcaag gttgagagcg cgcgtcaggt gttcccgctg
    28861 acggggcgtt atgcgcgcca gtttgccgca caccgtctgg cgctggtggg cgacgccgcg
    28921 cataccattc acccactggc ggggcagggc gttaatctcg gatttatgga cgccgcagag
    28981 ctgattgctg aactgaaacg gttgcatcgt cagggtaaag acatcgggca gtacatttac
    29041 ctgcgccgct atgaacgtag ccgcaagcac agcgcggcgc tgatgctggc tggtatgcag
    29101 ggttttcgcg atctcttttc cggcgctaat ccggcgaaaa aactgctgcg tgatattggt
    29161 ttgaaactgg ccgacacgct tcctggcgtt aagccgcaac ttatccgcca ggcaatggga
    29221 ttaaacgatt tgcctgaatg gctgcgttaa aaatttctcc tctgttgttt atttgatacc
    29281 catcacactt tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta
    29341 atttcacctc cgttttcgca ttatattttc taataccatg attttttgat ttagtgtttt
    29401 ttgacatttt tttagctctt aatattgtct tattcaaatt gactttctca tcacatcatc
    29461 tttgtataga aattggtgta ttttttggtt ttttattctg tcgcgatttt tgcatttttt
    29521 aaccataagc taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta
    29581 agttcaggca aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttt
    29641 gtggtgataa tttcaccatg aaaaagttgt cagccccgct tattcaatga ggacaagatg
    29701 gcacaacaga ctcctttgta cgaacaacac acgctttgcg gcgctcgcat ggtggatttc
    29761 cacggctgga tgatgccgct gcattacggt tcgcaaatcg acgaacatca tgcggtacgt
    29821 accgatgccg gaatgtttga tgtgtcgcat atgaccatcg tcgatctccg cggcagccgc
    29881 acccgggagt ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac caaaagcggc
    29941 aaagcccttt actcggggat gttgaatgcc tctggcggtg tgatagatga tctcatcgtc
    30001 tactacttta ctgaagattt cttccgcctc gttgttaact ccgccacccg cgaaaaagac
    30061 ctctcctgga ttacccaaca cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac
    30121 ctttccatga ttgccgtgca agggccgaat gctcaggcaa aagctgccac actgtttaat
    30181 gatgctcagc gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca ggtgggcgat
    30241 ctgtttattg ccaccactgg ttataccggt gaagcgggct atgaaattgc gctgcccaat
    30301 gaaaaagcgg ccgatttctg gcgtgcgctg gtggaagcgg gcgttaagcc ctgcggtctg
    30361 ggggcgcgtg acacgctgcg tctggaagcg ggcatgaatc tttatggtca ggagatggac
    30421 gaaactattt ctcctttagc cgccaacatg ggctggacta tcgcctggga accgacagat
    30481 cgtgacttta tcggtcgtga agccctggaa gtgcagcgtg agcatggtac agaaaaactg
    30541 gttggtctgg tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt acgctttacc
    30601 gatgcgcagg gcaaccagca tgaaggcatt atcaccagcg gtactttctc cccgacgctg
    30661 ggttacagca ttgcgctggc gcgcgtgccg gaaggtattg gcgaaacggc gattgtgcaa
    30721 attcgcaacc gtgaaatgcc ggttaaagtg acaaaacctg tttttgtgcg taacggcaaa
    30781 gccgtcgcgt gatttacttt tttggagatt gattgatgag caacgtacca gcagaactga
    30841 aatacagcaa agaacacgaa tggctgcgta aagaagccga cggcacttac accgttggta
    30901 ttaccgaaca tgctcaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg
    30961 gcgcaacggt tagcgcgggc gatgactgcg cggttgccga atcggtaaaa gcggcgtcag
    31021 acatttatgc gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc
    31081 cggaactggt gaacagcgaa ccgtatgcag gcggctggat ctttaaaatc aaagccagcg
    31141 atgaaagcga actggaatca ctgctggatg cgaccgcata cgaagcattg ttagaagatg
    31201 agtaacggct ttattcctct tctgcgggag aggatcaggg tgaggaaaat ttatgcctca
    31261 ccctcactct cttcgtaagg agagaggttc acaattcact gcacgtttca ggaaccatcg
    31321 ctcatgacac agacgttaag ccagcttgaa aacagcggcg cttttattga acgccatatc
    31381 ggaccggacg ccgcgcaaca gcaagaaatg ctgaatgccg ttggtgcaca atcgttaaac
    31441 gcgctgaccg gccagattgt gccgaaagat attcagcttg cgacaccacc gcaggttggc
    31501 gcaccggcga ccgaatacgc cgcactggca gaactcaagg ctattgccag tcgcaataaa
    31561 cgcttcacgt cttacatcgg catgggttac accgccgtgc agctaccgcc ggttatcctg
    31621 cgtaacatgc tggaaaatcc gggctggtat accgcgtaca ctccgtatca gcctgaagtc
    31681 tcccagggcc gccttgaagc actgctcaac ttccagcagg taacgctgga tttgactgga
    31741 ctggatatgg cctctgcttc tcttctggac gaggccaccg ctgccgccga agcaatggcg
    31801 atggcgaaac gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc ttccaatgtg
    31861 catccgcaaa cgctggatgt ggtccgtact cgtgctgaaa cctttggttt tgaagtgatt
    31921 gtcgatgacg cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct gttacagcag
    31981 gtaggcacta ccggtgaaat tcacgactac actgcgctta ttagcgaact gaaatcacgc
    32041 aaaattgtgg tcagcgttgc cgccgatatt atggcgctgg tgctgttaac tgcgccgggt
    32101 aaacagggcg cggatattgt ttttggttcg gcgcaacgct tcggcgtgcc gatgggctac
    32161 ggtggcccac acgcggcatt ctttgcggcg aaagatgaat acaaacgctc aatgccgggc
    32221 cgtattatcg gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat ggcgatgcag
    32281 actcgcgagc aacatattcg tcgtgagaaa gcgaactcca acatttgtac ttcccaggta
    32341 ctgctggcaa acatcgccag cctgtatgcc gtttatcacg gcccggttgg cctgaaacgt
    32401 atcgctaacc gcattcaccg tctgaccgat attctggcgg cgggcctgca acaaaaaggt
    32461 ctgaaactgc gccatgcgca ctatttcgac accttgtgtg tggaagtggc cgacaaagcg
    32521 ggcgtactga cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcc
    32581 gtggggatca cccttgatga aaccaccacg cgtgaaaacg taatgcagct tttcagtgtg
    32641 ctgctgggcg ataaccacgg gctggagatc gacacgctgg acaaagacgt ggctcacgac
    32701 agccgctcta tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt
    32761 aatcgctatc acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat
    32821 ctggcgctga atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc
    32881 gccgagatga tcccaatcac ttggacggaa tttgccgaac tgcacccgtt ttgcccgccg
    32941 gaacaggctg aaggttatca gcagatgatt gcgcagctgg ctgactggct ggtgaaactg
    33001 accggttacg acgccgtttg tatgcagccg aactccggcg cacagggcga atacgcgggc
    33061 ctgctggcga ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg
    33121 atcccggctt ctgcgcacgg aactaacccc gcttctgcac atatggcagg aatgcaggtg
    33181 gtggttgtgg cgtgtgataa aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg
    33241 gaacaggcgg gcgataacct ctcctgtatc atggtgactt atccttctac ccacggcgtg
    33301 tatgaagaaa cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac
    33361 cttgatggcg cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt tattggcgcg
    33421 gacgtttcgc acctcaacct gcataaaact ttctgcattc cgcacggcgg tggtggtccg
    33481 ggtatgggac cgatcggtgt gaaagcgcat ctggcaccgt ttgtaccggg tcatagcgtg
    33541 gtgcaaatcg aaggcatgtt aacccgtcag ggcgcagttt ctgcggcacc gttcggtagc
    33601 gcctctatcc tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa
    33661 aaagcaagcc aggtggcaat cctcaacgcc aactatattg ccagccgcct gcaggatgcc
    33721 ttcccggtgc tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt
    33781 cgcccgctga aagaagaaac cggcatcagc gagctggaca ttgccaagcg cctgatcgac
    33841 tacggtttcc acgcgccgac gatgtcgttc ccggtggcgg gtacgctgat ggttgaaccg
    33901 actgaatctg aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc
    33961 gcagaaattg accaggtgaa agccggtgtc tggccgctgg aagataaccc gctggtgaac
    34021 gcgccgcaca ttcagagcga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt
    34081 gcggtattcc cggcaggcgt ggcagacaaa tactggccga cggtgaaacg tctggatgat
    34141 gtttacggcg accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc
    34201 actgattcga gtattttcta aaggcgcttc ggcgcctttt tagtcagatg acaaagtaca
    34261 aaagtgctca gacagtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc
    34321 cggcactggc gcgaatattt accctcaccc cgaccctctc cctgaaaggg cgagggggaa
    34381 aagcgtgcca acattgaaga ttgagccagt ttgttagcaa tctcaaagat acgtcaacaa
    34441 attaattttt ctcggaaaaa caaatggcta tagcacttgt gaccggtggc agtcgcggca
    34501 tcgggcgggc aactgcatta ctgttggcgc aagaagggta tacggtggcg gttaattatc
    34561 agcaaaacct ccacgcggcg caggaagtga tgaacttaat aatgcaagcc ggtggcaaag
    34621 cattcgtgct ccaggcggat atcagcgacg aaaaccaggt cgttgcgatg tttacagcaa
    34681 tcgatcagca cgatgaaccg ctagcagcgc tggtcaataa cgccgggatc ttgtttaccc
    34741 agtgcaccgt tgaaaacctt accgcagagc gaatcaaccg agtactttcc accaacgtga
    34801 cgggatattt tctctgctgc cgcgaggcgg taaaacgcat ggcgcttaaa aatggtggca
    34861 gtggcggcgc tatcgtcaat gtctcttcgg tggcctcacg gttgggttcg ccaggggaat
    34921 atgttgatta cgcggcatcg aaaggggcga ttgattcgtt aaccaccgga ctatcgctgg
    34981 aagtcgccgc gcaggggatc cgcgttaact gcgtgcggcc agggtttatt tataccgaaa
    35041 tgcacgccag cggcggcgag cctggacgcg tcgatcgcgt taagtcgaac atccccatgc
    35101 agcgtggtgg acaggcagaa gaggtcgcgc aggccattgt ctggctacta agtgataaag
    35161 cctcttacgt cacgggaagt tttatcgatt tggcgggcgg gaaataaaac agggaagttg
    35221 tctgaccgga tgcaacaagt attgcatccg gtacttcatc gacttaaagc ttctcgccgt
    35281 tgctggcaat cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc
    35341 cagtaccgtc gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg
    35401 tagtgaatga cacgcagtcg atgcagcccc acggtgtgta gcccatcaga tccacaccat
    35461 cgtaagtcac tgcttttttc atctcttcaa tatgggcgcg caggtagtca atgcggtagt
    35521 cgtcgttgat gctgccatct tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa
    35581 taaacagcgg cctctgataa cgctcataca gttcgcaaag ggcatagcgc agacctactg
    35641 gatcaatctg ccagccccag tcagacgctt taacatacgg gttcggtacg ctgccttcaa
    35701 aaccagagat cgcatcgccg gtgccgcctt cggccttcac tgcgttggtc atgtaatagc
    35761 tgaaaccaag ataatcgcag gtgccttcgc gcagtacatc gagatcgccg tcttccattt
    35821 tgatattaaa tccgcgacgt tcccactcgt tcaatacata ggacgggtaa tagccgcata
    35881 gctgcacatc ggtaaagacg tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt
    35941 ccgggttaca tgagtaggga tagagcggca ccatcgccag catacagccg actttcatct
    36001 ccgggttaat gcgacgcgcg gctttcactg ccagggcgct ggcgacaaac tggtgatgca
    36061 gcacctggta catcgtctct tccgggttgt catgctcggt atacaccacg ccggagcagc
    36121 agtaaccaaa cagcggcgca cgccagttac gctggttgtt tatttcgttg aaggtcatcc
    36181 agtatttgac tttatgcttg tagcgttcga ataccacttc cgcgaaacgg acaaagaaat
    36241 caacgaattt acggttggtc cagctaccgt attgctgcac cagatgcagc ggcatttcaa
    36301 agtgggagag ggttatcacc ggttcgatgt tgtatttcag cagttcatcg aacagagagt
    36361 cgtaaaactt cagcccttct tcgtttggct gagcttcatc accttttgga aaaatacgcg
    36421 tccaggcgat ggaagtccga aaacatttga agcccatttc ggcaaatagc ttgatgtctt
    36481 ccttgtagtg accataaaaa tcaacggctt catggtttgg atagtatttt cctggcaaga
    36541 cttctttggt gatttcgcgc ggcacgccgt gtgcgccaca ggtcagaacg tcacaaatgc
    36601 tcggtccttt gccgcctttg ttccagccgc cttcgacctg atgagcggca acggcgccac
    36661 cccataagaa atctttcggt aaggtgagtt ttttcactat cataggctcc agatcagtca
    36721 attcttacag gaaatagtac catgcagcca aacgacatca cttttttcaa cgtttccagg
    36781 atgacattct ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca
    36841 aaacgggtga tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg
    36901 aagtcaccgc aacctcaacc gtaacgctgg atacactgac ggaaaaacat gcagagcagg
    36961 aaaatatgac cctgcctgaa ctgaaaaagg tcattgctga catctatccc ggtcagacac
    37021 aattttatat gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt
    37081 tcgcatttaa tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa
    37141 caggtgttca ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt
    37201 tctctggagt aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag
    37261 gaaattgcca acagcgtcag tcacggcatt gggctggtgt ttggtatcgt tgggctggtg
    37321 ttgctactgg ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc
    37381 ctctatggcg gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct
    37441 catcaacggg caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt
    37501 gccggaacct acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg
    37561 atgattgtta tctggagcct agcattgctg gggattctgt tcaaactgac catcgcgcac
    37621 cgattcaaaa ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta
    37681 atttatgaaa tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc
    37741 gtggtttatt cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc
    37801 atctggcacg gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat
    37861 attgggcagg cgtaatgggt atgcctttgt aggcctgata agacgcgtca gcgtcgcatc
    37921 aggcatcgga gcgcaaccgc cggatgcggc gtgaacgcct tatccgatct acgggcctcg
    37981 tccctgttgt gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca
    38041 ggcgataaat gcctgatgcg atacagagat ttactcttcg agcgaatacg gcagcggctc
    38101 gatatgcagt gtattcgcat cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt
    38161 attcatgacg acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt
    38221 accggtacga cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag
    38281 tcggctggca ctacctgcca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc
    38341 tcgcgccacc atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc
    38401 ctggagattg gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa
    38461 accagcttca atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg
    38521 cagtttatcg gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc
    38581 cgggtgttca aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga
    38641 aggcagttcg ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag
    38701 cagcacacgc tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag
    38761 ttcagtcagc tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc
    38821 gcggaacaga cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag
    38881 gtgctgatct tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc
    38941 gctgtccgcg ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg
    39001 taaacgggca gaagccgtag gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc
    39061 ctgagttgct taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt
    39121 gctttcacta cgcgatctgt gccttctgcg aggcgactgc caaaaaaatg aagcccttga
    39181 attgttgtga attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagt
    39241 cgaaaaacac gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca
    39301 ttaacaacaa agcccgaatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt
    39361 caatcatgtc gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct
    39421 ttattcgtct gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta
    39481 aaccggccga tgcagaactg gaaataatgg tccgactcat ccagacacgg aaccgggaac
    39541 gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg
    39601 attcatgggc tggttgccgc tgttatttta ctcatgccct ggccgctcag ttacaccccg
    39661 ttatggatgg tgttactttc gctggtggta tttgattgcg ttcgcagcca gcggtgtatt
    39721 aatgctcgcc agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag
    39781 gagtggagca tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt
    39841 tctgatggcg gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa
    39901 tggcgggatt tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa
    39961 agcatatgcg ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat
    40021 ttgctcgcac cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag
    40081 cgccagaccc acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata
    40141 tccttccgtt ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag
    40201 cataccgagc gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag
    40261 cgcaataatt ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc
    40321 ttcctggatt tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc
    40381 cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat
    40441 atcgcggatt ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc
    40501 catattcata acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat
    40561 cgctttagtc acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa
    40621 gtgaaacagg agctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat
    40681 ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg
    40741 catcaccgcg ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca
    40801 gaacggctgg agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc
    40861 cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc
    40921 gcttcaccga aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt
    40981 ttattacagg cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa
    41041 gtgttgtatg ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc
    41101 agcctgcgtc agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg
    41161 ttaggggaag aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg
    41221 ctgaatggtg tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga
    41281 cagaccttct ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag
    41341 ctgtcaccgc atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat
    41401 ttacgcgtgg tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg
    41461 catgtcgcta ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt
    41521 ctgaaaagaa cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg
    41581 ttttcaggct ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc
    41641 atcaaaagca gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac
    41701 agcggtgtgt tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac
    41761 gttagtggca cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg
    41821 cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt
    41881 actgatatta cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg
    41941 ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag
    42001 aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg
    42061 gcaggtaaaa gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt
    42121 ggcgtccagc ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt
    42181 ccgggttacc agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc
    42241 agcggtaaat aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa
    42301 cggcagactt gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggag
    42361 tacgcagtgc gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa
    42421 gcggtgtcga aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta
    42481 ttgtggtcgg tgatttcgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga
    42541 tgcgctcgct tggttgcagc aatatcgact acctggtacc aaaccgtttc gaagacggtt
    42601 acggcttaag cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca
    42661 cggtggataa cggtatttcc tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc
    42721 cggttattgt taccgatcac catttgccgg gcgacacatt acccgcagcg gaagcgatca
    42781 ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg
    42841 cgttttatct gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc
    42901 gcggcatcgc aattcctaac ctggcagaac tgctggatct ggtagcactg gggacagtag
    42961 cggacgtcgt gccgctggac gctaataatc gcattctgac ctggcagggg atgagtcgca
    43021 tccgtgccgg aaagtgccgt ccagggatta aagcgctgct ggaagtggca aatcgcgatg
    43081 cacaaaaact agccgccagc gatttaggtt ttgcgctggg accgcgtctc aatgctgccg
    43141 ggcgactgga tgatatgtcc gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag
    43201 cgcgcgtgct ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag
    43261 gaatgcaggt tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac
    43321 ccggcgggct ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt
    43381 cgcgtatcaa agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgacggta
    43441 cgctgaaagg ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat
    43501 tagacacact ctaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt
    43561 tgtcgctgga agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg
    43621 agtggctggc cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg
    43681 ccgaaatgac catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt
    43741 tcccggagcc gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac
    43801 gtcatttgaa ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt
    43861 ttaatgtcga taccgctctc tggccggata acggcgtgcg cgaagtgcaa ctggcttata
    43921 agctcgatat caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct
    43981 ggccaattta gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct
    44041 tatcaccgca ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg
    44101 taaataatcg cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac
    44161 tacgacgcca agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc
    44221 tggaacgaac ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt
    44281 gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg
    44341 gctgtagaag ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg
    44401 gaagaaaaac tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc
    44461 gactgttacc tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc
    44521 atgcttgagc gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc
    44581 gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat
    44641 tacgcttacg gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taaaagcccg
    44701 tttgactccg gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt
    44761 gatgatgata ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg
    44821 tccggcgcgg gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc
    44881 ccgaccggga tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag
    44941 gccatgaagc agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag
    45001 aaacaggcga tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat
    45061 gtccttgatg actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag
    45121 gccgtgctgg acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga
    45181 ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat
    45241 gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg
    45301 aacgatttcc gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag
    45361 aacgaagaac tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc
    45421 cgtattatgg gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg
    45481 tacgttgccc gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac
    45541 ctcggcgaca tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct
    45601 atccactgta ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc
    45661 cacggcttgc aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac
    45721 gatgaatccc gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc
    45781 atggtgaacc gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt
    45841 gccgctgcgc gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt
    45901 atcgcgccgg aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa
    45961 atcaaccgta acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg
    46021 atggaactct acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg
    46081 ttccgtactc tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg
    46141 ctggacttcg gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc
    46201 ccggaaaccg acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct
    46261 atcggtatcc acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa
    46321 gaagtggcag aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt
    46381 tctccgctgg cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc
    46441 atcggtggtc gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg
    46501 caacgcttcc tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac
    46561 gatgaagatt acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt
    46621 ggcatcgacc gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg
    46681 ttcccggcga tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt
    46741 cggggttttt tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt
    46801 cgcctgcatc accatccacg gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac
    46861 atcagctaaa tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact
    46921 ggtggtgcgt gcggcaaata ccggacacac ttcattttcc acaatgcctc tcggatcggt
    46981 ggcgcggtag cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc
    47041 ataacggcaa cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca
    47101 aaccgagtta gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa
    47161 taattgcccc ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc
    47221 cgtgtgtgcg gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat
    47281 gacgtgttcc gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa
    47341 ttgaagagtg aaatgattaa ccaagagagc gtttatcgac aaccgcccgt tttgagcgga
    47401 tgccgacagg taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg
    47461 gccattgcaa cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg
    47521 aatatagcgc gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg
    47581 catcagaagc cacatacgta ctgtcctgac ttctaaatct ttatcagaga cggattcctt
    47641 caccaggtca gcatgatgct gatagccatt aaagaagacg ttcagcacaa cggcgctaag
    47701 ggttgcgagc ataatgccgc tatgtagcag cggttgcagt acggccggta acttagaaaa
    47761 gaaatcgtga gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag
    47821 gttgtaacgg ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac
    47881 catgccgaac atcactagac cagcgccgcc cagcacaaat tgcggaatgg aggctaccag
    47941 caccgccatt tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac
    48001 ccagcggcta tgaacgcgca tcacgctaac caggccaacg ttttgagaaa atgacgtgtg
    48061 ggggaagctg ttaaacgtgc cgcctatcat tgtccctacg ccatcgacac gcagcccgca
    48121 aataatatcg tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc
    48181 catcgactcg ataaacacga tgattaacac ggcagtcatg gtcagaatgg aaacgggatc
    48241 gaaaatcggc ataccaaacg acatcggggt gacaatcgca aaccacgagg catcatgtag
    48301 cccggataaa ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag
    48361 taatacggcg acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat
    48421 taagacggca aaggagatac ctaaataaac gggattacca tattgcggat tccctttacc
    48481 tccagcggcc cagtcaatac ccacctgaat gatgctaagc ccaatagaag taataaccac
    48541 accggtaacc agtggcggga ataaaggcat caagcgacca ataagtggcg ctaataatgt
    48601 ggtgataaaa cccgcggcga tagtggcacc aaatatcccc agcaggccga tatccgggtt
    48661 catcccaatg gctatcattg gtgttacagc agcaaaggtc accgacataa tcaccggcag
    48721 gcggatcccc ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca
    48781 aaagagatcc gagctaatga gcatcgcaat agcatctttt gagaggccca gtcggtcacc
    48841 aatcataaga ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag
    48901 tatgatcagc tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc
    48961 agaagatgag ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca
    49021 gaataaaaag caaccgttta cgttaatgaa actgtctaaa cacatgctcg tggagtgcca
    49081 gtttttgcga ggaaggggag ttagagacag gaattgcgga ggcaatcaca taatcgtgct
    49141 taaaccacga aaatgacaag agaaactgac aatggaatta tct
//
LOCUS       DAJTUP010000018        49031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTUP010000018 DAJTUP010000000
VERSION     DAJTUP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.018"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1154)
                     /gene="setB"
                     /locus_tag="OO468_001082"
     CDS             complement(<1..1154)
                     /gene="setB"
                     /locus_tag="OO468_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR6258282.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLGSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1522..2652
                     /gene="fruB"
                     /locus_tag="OO468_001083"
     CDS             1522..2652
                     /gene="fruB"
                     /locus_tag="OO468_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR6258283.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2652..3590
                     /gene="fruK"
                     /locus_tag="OO468_001084"
     CDS             2652..3590
                     /gene="fruK"
                     /locus_tag="OO468_001084"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR6258284.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3607..5298
                     /gene="fruA"
                     /locus_tag="OO468_001085"
     CDS             3607..5298
                     /gene="fruA"
                     /locus_tag="OO468_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR6258285.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5722..6663
                     /locus_tag="OO468_001086"
     CDS             5722..6663
                     /locus_tag="OO468_001086"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR6258286.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6651..7589
                     /gene="psuG"
                     /locus_tag="OO468_001087"
     CDS             6651..7589
                     /gene="psuG"
                     /locus_tag="OO468_001087"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR6258287.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7683..8933
                     /locus_tag="OO468_001088"
     CDS             7683..8933
                     /locus_tag="OO468_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6258288.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9004..9698)
                     /gene="yeiL"
                     /locus_tag="OO468_001089"
                     /pseudo
     CDS             complement(9004..9698)
                     /gene="yeiL"
                     /locus_tag="OO468_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9829..10770
                     /gene="rihB"
                     /locus_tag="OO468_001090"
     CDS             9829..10770
                     /gene="rihB"
                     /locus_tag="OO468_001090"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR6258289.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10870..12120
                     /locus_tag="OO468_001091"
     CDS             10870..12120
                     /locus_tag="OO468_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR6258290.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12176..13264)
                     /locus_tag="OO468_001092"
     CDS             complement(12176..13264)
                     /locus_tag="OO468_001092"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR6258291.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13267..14124)
                     /gene="nfo"
                     /locus_tag="OO468_001093"
     CDS             complement(13267..14124)
                     /gene="nfo"
                     /locus_tag="OO468_001093"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR6258292.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14198..15247)
                     /locus_tag="OO468_001094"
     CDS             complement(14198..15247)
                     /locus_tag="OO468_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR6258293.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15346..16227
                     /locus_tag="OO468_001095"
     CDS             15346..16227
                     /locus_tag="OO468_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6258294.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16433..17902
                     /gene="lysP"
                     /locus_tag="OO468_001096"
     CDS             16433..17902
                     /gene="lysP"
                     /locus_tag="OO468_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR6258295.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18194..20185
                     /gene="cirA"
                     /locus_tag="OO468_001097"
                     /pseudo
     CDS             18194..20185
                     /gene="cirA"
                     /locus_tag="OO468_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20217..21052)
                     /gene="yeiG"
                     /locus_tag="OO468_001098"
                     /pseudo
     CDS             complement(20217..21052)
                     /gene="yeiG"
                     /locus_tag="OO468_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21310..21978
                     /gene="folE"
                     /locus_tag="OO468_001099"
     CDS             21310..21978
                     /gene="folE"
                     /locus_tag="OO468_001099"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR6258296.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21995..23071
                     /locus_tag="OO468_001100"
     CDS             21995..23071
                     /locus_tag="OO468_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR6258297.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23213..24253
                     /gene="galS"
                     /locus_tag="OO468_001101"
     CDS             23213..24253
                     /gene="galS"
                     /locus_tag="OO468_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR6258298.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24533..25531
                     /gene="mglB"
                     /locus_tag="OO468_001102"
     CDS             24533..25531
                     /gene="mglB"
                     /locus_tag="OO468_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR6258299.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25592..27112
                     /gene="mglA"
                     /locus_tag="OO468_001103"
     CDS             25592..27112
                     /gene="mglA"
                     /locus_tag="OO468_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR6258300.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27128..28138
                     /gene="mglC"
                     /locus_tag="OO468_001104"
     CDS             27128..28138
                     /gene="mglC"
                     /locus_tag="OO468_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR6258301.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28174..29387)
                     /gene="preA"
                     /locus_tag="OO468_001105"
                     /pseudo
     CDS             complement(28174..29387)
                     /gene="preA"
                     /locus_tag="OO468_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29381..30619)
                     /gene="preT"
                     /locus_tag="OO468_001106"
     CDS             complement(29381..30619)
                     /gene="preT"
                     /locus_tag="OO468_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR6258302.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30813..31052)
                     /locus_tag="OO468_001107"
     CDS             complement(30813..31052)
                     /locus_tag="OO468_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR6258303.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31055..31774)
                     /gene="sanA"
                     /locus_tag="OO468_001108"
     CDS             complement(31055..31774)
                     /gene="sanA"
                     /locus_tag="OO468_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR6258304.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31924..32808)
                     /gene="cdd"
                     /locus_tag="OO468_001109"
     CDS             complement(31924..32808)
                     /gene="cdd"
                     /locus_tag="OO468_001109"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR6258305.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32938..33633)
                     /locus_tag="OO468_001110"
     CDS             complement(32938..33633)
                     /locus_tag="OO468_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR6258306.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33630..34028)
                     /locus_tag="OO468_001111"
     CDS             complement(33630..34028)
                     /locus_tag="OO468_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR6258307.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34267..35214
                     /gene="dusC"
                     /locus_tag="OO468_001112"
     CDS             34267..35214
                     /gene="dusC"
                     /locus_tag="OO468_001112"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR6258308.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35585..35668)
                     /gene="yohP"
                     /locus_tag="OO468_001113"
     CDS             complement(35585..35668)
                     /gene="yohP"
                     /locus_tag="OO468_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR6258309.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35892..37328
                     /gene="mdtQ"
                     /locus_tag="OO468_001114"
                     /pseudo
     CDS             35892..37328
                     /gene="mdtQ"
                     /locus_tag="OO468_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37381..38142
                     /locus_tag="OO468_001115"
                     /pseudo
     CDS             37381..38142
                     /locus_tag="OO468_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38259..38837)
                     /locus_tag="OO468_001116"
     CDS             complement(38259..38837)
                     /locus_tag="OO468_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6258310.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39007..39594
                     /locus_tag="OO468_001117"
     CDS             39007..39594
                     /locus_tag="OO468_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR6258311.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39768..40700
                     /gene="pbpG"
                     /locus_tag="OO468_001118"
     CDS             39768..40700
                     /gene="pbpG"
                     /locus_tag="OO468_001118"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR6258312.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40738..42453)
                     /gene="dld"
                     /locus_tag="OO468_001119"
     CDS             complement(40738..42453)
                     /gene="dld"
                     /locus_tag="OO468_001119"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6258313.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42649..44946
                     /gene="bglX"
                     /locus_tag="OO468_001120"
     CDS             42649..44946
                     /gene="bglX"
                     /locus_tag="OO468_001120"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR6258314.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45198..46115
                     /gene="osmF"
                     /locus_tag="OO468_001121"
     CDS             45198..46115
                     /gene="osmF"
                     /locus_tag="OO468_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR6258315.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46122..47279
                     /gene="yehY"
                     /locus_tag="OO468_001122"
     CDS             46122..47279
                     /gene="yehY"
                     /locus_tag="OO468_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR6258316.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47272..48198
                     /gene="yehX"
                     /locus_tag="OO468_001123"
     CDS             47272..48198
                     /gene="yehX"
                     /locus_tag="OO468_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR6258317.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48203..48934
                     /gene="yehW"
                     /locus_tag="OO468_001124"
     CDS             48203..48934
                     /gene="yehW"
                     /locus_tag="OO468_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR6258318.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48915..49022)
                     /locus_tag="OO468_001125"
     CDS             complement(48915..49022)
                     /locus_tag="OO468_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR6258319.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
BASE COUNT        11842 a        12087 c        13167 g        11935 t
ORIGIN      
        1 aacagagtgg cgataatcat caccatcgca aaccagaaca cagcgtgata attccagatc
       61 tcggcgacga tgcccgccac tgatcctgcg atgatccagc ccacgcgcga cgtgttggta
      121 tagagcgtgg tggctgaacc tgcctgaccg ggcatcaaat cctgaaaata gagcatcccg
      181 atgccgccca gaatgccaat aaaaatagca tttagcagct gcaagcccaa cagaatggca
      241 ggtgaatgcg ccatcagcat tcctgcgtaa aaacagacgc cacccacggc agcaacgcgc
      301 attaagaaac gcttacccag acgtttggcg aaatatccgg caatcaacat cgtcgggatt
      361 tccagcccgg cggcggtccc catcatcaca ccggccagtt tctcgggaag atgcagttcg
      421 ttgataataa atagcggcat gttgatgatg tacaggctgt tcgagcccca catcaatgta
      481 caaatgacaa acagcagtag cgtatcgcga cggttacggc gcggcgcttc aaccgtgccg
      541 gtcgccagcg gaagctcttt ttgcatcgac ggtaaaaaca gccacaccat cacgccgcaa
      601 acaataaacg ctaccgctgc gctcagatac attaccgtaa agctgaaacc catcgctaac
      661 gcataagcca gcggtgggcc aatgacccat gccagtgaaa cctgagcgcg taaaaaagag
      721 ctgaacatca ccgcctcacg tccggttttg tcggcatgtt cacgggcaag ggcaaacatt
      781 tgcgggttag cggtcgagcc aaagctgcca agaaagacgc caacgaatag caaaacaaag
      841 tagttgcgat tccaagcaaa aagggtgcag gccagcacgc ctaacaggca gcaaaagaca
      901 atcagcgatt tgcgatcgcc gcgcttatca gagcgcccag cgagaaactg acttaccaga
      961 atcccaatga cagcgctgcc ggtaaagaag aatcccacca tcgccggacg ggcatgtact
     1021 tcatcggtaa ggaaaatact gagtgtcggg gtttgcagag cacccgcaat accggtgaga
     1081 aaggcaacga ttaaaaacgc cgtcgaggtc aggtcaaacg atttcgcgct ggagactgcg
     1141 ggggagttat gcatgctggt cgatcactgg aagaggaaga cgcggagttt acgctgcttg
     1201 tcagaaaata aacagacagc gaaatcaaaa gcgccacgaa taatcaaaat gccatttcac
     1261 tgttgctgat tggctgctga cgctgctgaa atgaaattgc tgataacgga ttttcccatc
     1321 agcaattagg aaaaatgaca aaaaattgtg cagcacatca aacttttgct cataacttta
     1381 cggctttcct tgcgtgctga aacagaagta ttatgctttc ttgaaacgtt tcagcgcgat
     1441 cttgtcttta accctaagct aggttggcgc tttttttctc atagaggctg aatcgtttca
     1501 attcagcaag agaggagaac tatgttccag ttatccgtac aggacatcca tccgggcgaa
     1561 aaggccggag acaaagaaga ggcgattcgc caggtcgctg cggcgctggt gcaggccggt
     1621 aatgtagcag aaggctacgt caatggcatg ctggcgcgcg aacagcaaac ctcaacgttc
     1681 ctcggcaatg gtattgctat tccacacggc actaccgaca cccgcgatca ggtgctgaaa
     1741 accggcgttc aggtatttca gttcccggaa ggcgtcacct ggggtgacgg tcaggtagcg
     1801 tacgtggcaa tcggtattgc tgccagctcg gataaacatc tgggcctgct acgccagctg
     1861 acccacgtac tgagcgatga ttccgttgct gaacaactga agtcagcaac aacagcagaa
     1921 gaacttcgcg cattactgat gggcgaaaag cagagtgagc agctgaagct cgacaacgaa
     1981 atgctgacgc tggatatcgt cgccagcgat ctgctgactc ttcaggcgct gaacgctgcg
     2041 cgtctgaaag aggcgagggc agttgacgcc actttcgtca ccaaagccat caatgaacaa
     2101 ccgctgaatc tcggacaggg tatctggctg agcgatagcg ccgaaggcaa tctgcgtagt
     2161 gcgattgcgg taagccgtgc ggcaaatgct tttgatgtgg acggcgaaac ggcagccatg
     2221 ctggtgagtg tggcgatgaa tgacgatcag cccatcgcgg ttcttaagcg tctcgctgat
     2281 ttgttgctcg acaataaagc tgaccgcttg ctgaaagcgg atgcggcaac gttgctggcg
     2341 ctgctgacca gcgatgatgc gccgaccgac gacgtgttaa gcgcggagtt tgtggtgcgc
     2401 aatgaacacg gcctgcatgc tcgtccaggt accatgctgg tcaataccat taaacaattt
     2461 aacagtgata ttaccgtgac aaaccttgat ggcaccggca aaccggcaaa cggacgtagt
     2521 ctgatgaaag ttgtggcact tggcgttaag aaaggtcatc gcctacgctt taccgcccag
     2581 ggtgcagatg ctgaacaggc gctgaaagcc attggcgacg ctatcgctgc tggtcttggg
     2641 gagggcgcat aatgagcaga cgtgttgcta cgatcaccct taatccggct tatgaccttg
     2701 ttggtttctg cccggaaatt gaacgcggcg aagtgaacct ggtgaaaacc accggtctgc
     2761 atgcggcggg taaaggcatc aacgtggcca aagtattaaa agacctggga attgatgtca
     2821 ccgttggcgg cttcctgggt aaagacaatc aggatggttt tcagcaactg ttcagcgagc
     2881 tgggcattgc caaccgtttc caggttgtac aggggcgcac ccgaattaac gttaagctga
     2941 cggaaaaaga cggcgaagtg accgacttca acttctcggg tttcgaagtc acccccgccg
     3001 actgggaacg ctttgtgact gattctctga gctggctcgg tcagttcgat atggtctgtg
     3061 tcagcggaag cttaccgtca ggcgtcagcc cggaagcgtt caccgactgg atgactcgcc
     3121 tgcgtagtca gtgtccttgc attatctttg atagtagccg tgaagcgtta gtagcaggtt
     3181 tgaaagcggc accgtggctg gtgaaaccta accgccgcga gttggaaatc tgggcaggcc
     3241 gtaaactgcc tgaaatgaaa gatgtgattg aagctgcaca tgcgctacgt gaacaaggta
     3301 ttgcgcatgt tgttatttca ctgggtgccg aaggcgcgct ttgggttaat gcctccggcg
     3361 aatggatcgc taaaccaccg tcagtcgatg tcgtaagcac cgttggcgca ggggattcta
     3421 tggttggtgg cctgatttat ggcttgctga tgcgtgaatc cagtgaacac acactgcgtc
     3481 tggcgacagc tgttgcagcc ctggcggtaa gtcaaagcaa tgtgggtatt accgatcgtc
     3541 cgcagttggc cgcaatgatg gcgcgcgtcg acttacaacc ttttaactga cagcaggaga
     3601 ggcataatga aaacgctgct gattattgac gctaatctcg gtcaggcacg cgcctatatg
     3661 gcgaagaccc tgctgggcgc ggcggcgcga aaagcaaaac tggaaatcat cgacaatccg
     3721 aacgacgcag aaatggcgat tgttctcggt gattccatcc cgaatgacag cgcgctgaac
     3781 ggtaaaaatg tctggctggg tgatatttcc cgggcagttg cgcaccctga gctgttcctg
     3841 agtgaagcca aaggccatgc gaaaccttac actgcgccgg tcactgcgac agcaccggtt
     3901 gccgccagcg gtccgaaacg cgtagttgcg gtgactgctt gcccgactgg cgtagcacac
     3961 acctttatgg cggctgaagc cattgaaacc gaagcgaaaa aacgtggctg gtgggtgaaa
     4021 gttgaaaccc gtggttctgt tggcgcgggt aatgcaatca ctcccgagga agtagccgca
     4081 gcggatctgg tgattgtggc ggcagatatc gaagtggatc tggcgaaatt tgctggtaaa
     4141 ccgatgtatc gtacctctac cggtctggcg ctgaagaaaa cctcgcagga actggataaa
     4201 gcggttgctg aagcaacgcc gtatgaaccg gcgggcaaag ctcaaacggc gaccactgaa
     4261 ggtaagaaag agagtgcagg cgcttatcgt cacttgctaa cgggcgtctc ttacatgctg
     4321 ccgatggtcg ttgctggtgg tctgtgtatc gcgctttctt ttgcttttgg tatcgaagcg
     4381 tttaaagagc cgggtacgtt ggcagcggcg ctgatgcaga ttggtggtgg ttcagccttt
     4441 gcgctgatgg tgccggtact ggcaggttat attgccttct ccattgccga tcgtccgggc
     4501 ctcacgccgg gtctgattgg cggtatgctg gcggtcagca ccggttctgg cttcattggc
     4561 ggtattattg cgggcttcct ggctggttac attgcgaagt taatcagtac gcaactgaaa
     4621 ctgccgcaga gtatggaggc gctgaaaccg atcctgatca ttccgctaat ttccagtctg
     4681 gtggtcggtc tggcgatgat ctacctgatc ggtaaaccag ttgctggcat tctcgaaggg
     4741 ctgactcact ggctgcagac catggggact gcgaatgcgg ttctgctggg ggcgatcctc
     4801 ggtggcatga tgtgtactga catgggcggt ccggtaaaca aagcagcgta cgcattcggt
     4861 gtgggtctgc tgagtactca aacctatggc ccgatggcgg cgattatggc ggcaggtatg
     4921 gtgccaccgc tggcaatggg cctggcaaca atggtggcgc gtcgcaaatt cgacaaagcg
     4981 cagcaggaag gtggcaaagc cgctctggta ttgggactgt gcttcatttc ggaaggtgca
     5041 attccgtttg ctgctcgtga tccgatgcgt gtgctgccgt gctgtatcgt gggtggggcg
     5101 ctgactggcg caatctcaat ggcgattggt gcgaaactga tggcaccgca cggtggtctg
     5161 tttgttctgc tgatccctgg cgctattacg ccggtattgg gttacctggt agcaattatt
     5221 gccggtacgc tggtggcggg tttggcctat gccttcctga aacgtccgga agtggacgca
     5281 gtagcgaaag cagcgtaata aaaggtgtgt tacagggcag aaatttcctg ccctgtaaca
     5341 tttgatgagc gcaaagagaa tgaccagctt gtgaatgtaa ttttatgaat ttaaatggtt
     5401 ttatctttca gtagaaactt caaacgttca tcaaacacta aggttatttc ctcaagggca
     5461 gattattttc tgcccttttt tagtgttcct gttctgtgca tcccgtcaca aattcctcgc
     5521 ttttgtaatt caggcattta atcgcatttt gtgagcctgc tcaaatctgc ccattttgcc
     5581 tctctggcgg taatttactt tacgtaaaac atgcatacta tgagcacatg tttaaatatg
     5641 aacacatgtt taaaccgtct ttgcagtatc tatattcttg tggattgaca gggttggtaa
     5701 ttttttacca gaggacgtgc tatgcgcgaa aaggattatg tcgtaattat aggttcggcg
     5761 aatattgatg tcgccggata ttcacatgaa tcattaaatt atgcggattc aaatccaggt
     5821 aaaataaaat ttacgcctgg tggagtaggg cgcaatattg cacaaaacct ggcgttgctg
     5881 ggtaacaaag cctggctact gagcgccgta ggcagtgatt tttatggtca atcgctgcta
     5941 acgcaaacca atcaatctgg tgtttatgtc gataaatgcc tgattgtgcc gggagaaaat
     6001 acgtcgagtt atttatcatt actcgataat accggtgaaa tgctggttgc tataaatgac
     6061 atgaatatta gcaacgctat tacagctgaa tatctcgcac agcaccgtga atttattcag
     6121 agggcaaagg tcattgtcgc ggactgtaat atcagtgaag aggcactggc atggattctg
     6181 gataatgccg ccaacgtacc cgtatttgtc gatccggttt cagcgtggaa atgtgtcaaa
     6241 gtgcgcgacc gtctaaatca gaaccacact ctgaagccaa accgccttga agcggaaacc
     6301 ctgagtggga ttgcgctgtc agggcgtgac gatgtggcaa aagttgctgc ctggttccat
     6361 caacatggcc tgaaccgact ggtattgagc atgggcggcg acggcgttta ttacagcgat
     6421 atcagcggtg aaaatggctg gtctgcgccg atcaaaacca atgttattaa tgttaccgga
     6481 gcgggcgatg ccatgatggc gggacttgct tcgtgttggg tagacggaat gccgtttgcc
     6541 gaatctgttc gtttcgcaca gggatgttcg tcaatggcgc tctcctgtga atacaccaat
     6601 aaccccgatt tatcgattgc caacgttata tcgttagtgg agaacgcaga atgtctgaat
     6661 taaaaatttc ccctgaatta ttacaaattt ctccggaagt gcaggacgct ttaaaaaaca
     6721 aaaaaccggt tgtggcgctg gaatcgacca ttatttctca cgggatgccg ttcccacaaa
     6781 atgcccagac cgcaattgaa gtagaagaaa ctattcgtaa acagggcgca gtacctgcca
     6841 ctatcgccat tattggcggc gtgatgaaag tgggtttaag caaagaaaaa attgaattac
     6901 tgggtcgtga agggcataac gtgaccaaag ttagtcgtcg cgatttacct tttgttgttg
     6961 ccgccggaaa aaatggcgca accactgtgg cttcaacgat gattattgcg gcgcttgccg
     7021 gaattaaagt atttgccacc gggggaattg gtggtgtgca tcgcggggcg gaacatacct
     7081 tcgatatttc tgccgatttg caagaactgg caaatactaa tgtcaccgtt gtttgtgccg
     7141 gggcgaaatc tattctcgat ttaggattaa ccactgagta tttagaaacc ttcggtgtgc
     7201 cgttaattgg ctatcagact aaagcgctgc ctgcgttttt ctgccgtacc agctcgtttg
     7261 acgtcagcat tcgtctcgac agcgccagtg aaattgcccg tgcaatggcg gtgaaatggc
     7321 aaagcgggct gaacggtggc ctcgtggtag cgaacccgat cccggaacag tttgcgatgc
     7381 cggaggaatc tatcaatgca gccatagatc aagccgtcgc cgaagccgaa gagcagggcg
     7441 ttattggtaa agaaagtaca ccgttcctgc tggcgcgcgt tgctgagctg accagcggtg
     7501 acagcctgaa atccaatatc cagctggtgt tcaacaacgc cattctggcg agtgaaattg
     7561 ccaaagaata ccagcgtctc gcgggttaat ttctgacggg cagaaagccg tcgcccgtac
     7621 tcatcaagca taccaaaacc tggcgctgac gccgggttta ctggcatgaa gggaaaatca
     7681 ttatggatat aatgagaagt gttgtgggga tggtggtgtt actggcaata gcatttctgt
     7741 tgtcagtgaa taaaaagagc atcagtttgc gcacggttgg agccgcactg ctgctgcaaa
     7801 tcgctattgg tggcatcatg ctctacttcc caccgggaaa atgggcagta gaacaggcgg
     7861 cattaggcgt tcataaagtg atgtcttaca gtgatgccgg tagcgccttc atttttggtt
     7921 cgctggttgg gccgaaaatg gatgtcctgt ttgacggtgc gggttttatc ttcgcctttc
     7981 gcgtacttcc ggcgattatt ttcgttactg cgctcatcag tctgctgtac tacattggcg
     8041 tgatggggct gctgattcgc atccttggca gcattttcca gaaagccctc aacatcagca
     8101 aaatcgaatc ttttgttgcg gttaccacta ttttcctcgg gcaaaatgag atcccggcga
     8161 tcgttaaacc gtttatcgat cgcgtgaatc gcaacgagtt gtttaccgca atttgtagcg
     8221 ggatggcgtc cattgctggt tcggtgatga ttggttatgc cggaatgggc gtaccaattg
     8281 actacctgtt agcggcatcg ctgatggcga tccccggcgg tattttgttt gcacgtattc
     8341 ttagcccggc aaccgagcct tcgcaggtca catttgaaaa tctgtcgttc agcgaaacgc
     8401 cgccaaaaag ctttatcgaa gcggcggcga gcggtgcgat gaccgggctg aaaatcgccg
     8461 ctggtgtagc gacggtggta atggcgtttg tcgcaattat tgcgctgatc aacggcatta
     8521 tcggcggaat tggcggctgg tttggtttcg ccaatgcctc tctggaaagt atttttggct
     8581 atgtgctggc accgctggcg tggatcatgg gtgtggactg gagtgatgcc aaccttgctg
     8641 gtagcctgat tgggcagaaa ctggcgatta acgaattcgt cgcttacctg agtttctccc
     8701 catacctgca aacgggcggc acgctggaag tgaaaaccgt ggcgattata tcctttgcgc
     8761 tttgtggttt tgctaacttt ggttctatcg gtgttgtcgt tggcgcattt tcggctattt
     8821 cgccaaaacg tgctccagaa atcgcccagc ttggtttacg ggcgctggca gcagcaacgc
     8881 tttccaacct gatgagtgcg actattgccg ggttctttat tggtctggcg taatgctcga
     8941 tttgtagacc ggataaaacg tattaagcgt tgcatccggc aatcgcgcta ttgcaaataa
     9001 tttttactgc atcatcccgg agaatttatt ctccgggtcc atctccagcg ccagtcctga
     9061 caactgcttt ctgtttttaa ttagatatcc tttcttttaa ttaataaacc gtcgtgaatg
     9121 aattgcgcga gaacatataa aagatgtcga taagaaacgc ctaaatactc tgccgcttgc
     9181 gtatgctttt cgtgataaag atcaccttct tgtgagagta atataaatgc tgccaggcga
     9241 ttcactaacg gaaatgattg attctgagtt aaagaaacaa tattacgata atttttatga
     9301 cttaaggtga cgcaaagttt tcgtaaaaat agggtgtcgt ttaataacag cggacggtaa
     9361 tgtttcatag ggagcgcaag gcaccaacac tcttcaatag cctgcaccgc acgcggttca
     9421 tggtctttgt cgattaactc aatctcgcca ataaaacagg gagcggcaaa gaaatcgatc
     9481 agcgacacgc gaccattggc aagcgtggcg taaagcctgg cgcggcctcg cgtcagataa
     9541 aacagccatg acggctgttg cccttcctgc acaatgtagt cacgcgctaa aaaatgaaac
     9601 agccgcgtat cggctgaaac atccgttgaa aagcaatcct taaacgcaga ttcactcatc
     9661 aattgctgct taaggtcatt attgtggatt tctttcacga tgtttatccg ctatgagatt
     9721 tctcatattt agcatacgta cgctgttgtt aagattcaat cattggcccg gtcgaatttt
     9781 ctttgctgta gaaaaaaata acgcaactgg aaacagagga aataaacaat ggaaaagaga
     9841 aaaattattc tggattgtga gccgggtcat gatgatgcta ttgcaatgat gatggcagcg
     9901 aaacatccgg caatagattt attaggcatc actattgtag cgggtaatca gacgcttgat
     9961 aaaacattaa ttaatggcct gaatgtttgc cagaaactgg agattaatgt tccggtttat
    10021 gcggggatgc cgcaacccat tatgcgtaaa caaatcgttg ccgataatat tcacggtgaa
    10081 accggactgg atggcccggt attcgagccg ctgacccgcc aggcagaaag tacccatgcg
    10141 gtgaaatata tcatcgatac cctgatggca agcgatggcg atatcactct ggtgccggtt
    10201 ggtccgcttt caaatatcgc ggtggcaatg cgtatgcaac ccgcaatcct gcccaaaatc
    10261 cgtgaaattg tgctaatggg cggcgcttac ggtacaggca acttcacccc atctgccgag
    10321 ttcaatatct ttgccgaccc ggaagccgcc cgcgtggtgt tcacctccgg cgttccatta
    10381 gtgatgatgg gcctcgatct taccaaccag accgtttgca ccccggatgt aattgctcgg
    10441 atggaaaggg taggcggccc tgccggagag ctgttcagcg acatcatgaa cttcactctc
    10501 aaaacgcagt tcgaatacta cggcctggct ggcggcccgg tgcatgacgc cacctgcatt
    10561 ggttatctga tcaaccctga tggcattaaa acccaggata tgtacgtcga agtggacgtc
    10621 aacagcggcc cttgctacgg gcgtacggtc tgcgacgaac tgggcgttct tggcaagccc
    10681 gctaatacca aagtcggcat cactattgat accgactggt tctggggctt agtcgaagag
    10741 tgcgtgcgcg gctacatcaa aacccattaa gtgttgataa caagccggga cagtatcgca
    10801 agatgcaggc ccggctattt ctgatgggca gaaagccgtc gcccaaaatg acatgaagag
    10861 ataatcacta tggatgtcat gagaagtgtt ctgggaatgg tggtactgct ggcaattgcg
    10921 tttttactgt cagtaaacaa gaagaagatc agcctgcgta ccgttggcgc ggcgttagtg
    10981 ttacaggtcg tgattggcgg gattatgctt tggttaccgc cagggcgttg ggtcgcagaa
    11041 aaagtcgctt ttggcgtcca caaagtgatg gcgtacagcg acgcaggtag cgcatttatc
    11101 ttcggttctc tggtcggacc gaaaatggat accttatttg atggcgcagg atttatcttt
    11161 ggtttcaggg tattaccggc aattatcttc gtcactgcac tggtgagtat tctctactac
    11221 atcggtgtga tggggatctt aattcgcatt ctcggcggta tcttccagaa agctttaaat
    11281 atcagcaaga tcgagtcatt cgtcgcggtc accaccattt tcctcgggca aaacgaaatt
    11341 ccggcgatcg tcaaaccctt tatcgatcgt ctgaatcgca atgaattatt tacagcgatt
    11401 tgtagtggca tggcctcgat tgctggttcg acaatgattg gttacgccgc actgggcgta
    11461 cctgtggaat atttgctggc ggcatcgtta atggcgatcc ctggcgggat cttgtttgcc
    11521 cgcctgttaa gcccggctac ggaatcttcg caggtttctt ttaataacct ctctttcacc
    11581 gaaacaccgc caaaaagcat tattgaagcc gatgcgacag gggcaatgac cgggctgaaa
    11641 atcgccgccg gtgtagcgac agttgttatg gcatttgtcg ccatcattgc gttaattaac
    11701 ggtattatcg gcggcgttgg cggctggttt ggttttgaac atgcctcgct ggagtccatt
    11761 ttaggttacc tgctggcccc acttgcgtgg gtaatgggtg ttgactggag tgatgcaaat
    11821 cttgccggga gtttgattgg acagaaactg gcaataaatg aatttgtcgc ttatctcaat
    11881 ttctcaccct atctgcaaac ggctggcact ctggatgcta aaaccgtggc gattatttcc
    11941 ttcgcgttgt gcggtttcgc taactttggt tctatcgggg tggtggtggg ggcgttttct
    12001 gcggttgcgc cacaccgtgc gccggaaatc gcccagcttg gtttacgcgc gctggcggcg
    12061 gcgacacttt ctaatctgat gagtgccacc attgccgggt tctttattgg tttagcgtag
    12121 tgtgcaattt atgagccgga ttagatgcgg tcaagcgtcg aatccggcaa cattgttaca
    12181 ccattggcac taatgacagc gcgttatcgg cagacagggt agggttgttt aagctgccgc
    12241 tggcgcgtga aattgccgcg caggccatcg caaaacgggc gctgtcacgg aaattgtttc
    12301 cctccagaaa gctatatacc agacccgcca taaaaccatc gtcagcacca aaactgtcta
    12361 ccgtcgtatg cgctggcaca gtcagcaaaa attgttcacc atccttttcg ctgcaataaa
    12421 ctgactcatc gggcaaataa acaaacagtt gctgaacacc ttgctgatgc aatgcattca
    12481 ctgcggcatt acggtcagca tcgctggtga tcgcctgtcc ccataaaatt tccagctccg
    12541 gtaaagtggg tttcagggtg tgaatatgcg ccagccagtg tttgattttg cccgctttga
    12601 attctgaaac ggtatcgaca aacaccggga tctcatcagc gagggtaaag acccattcca
    12661 gcgcctcggc tgtcaggttg caatcagcca gtaccacgcc cgcatgacga agtaaatcgc
    12721 gcgacccgtt cagtagttgc ggtgtcaact gttccagcag atgggtatcg ttaattgcca
    12781 gcacggtttg atcgtctcga ttggcaattg ccagatacgt cgatgtgctt tgaccatgca
    12841 aacgaacgca gccggagaca ttcacgccgg cacggcgcgt ttcttccagg agcatttcgc
    12901 cataaaagtc atcgccaatc actgaaagca aatggacgtc acggcctaac agcgccagat
    12961 tgtgggcgat attgcgtcca acgccgcctg ccgaacaatg aattgtaccg ggatgagaag
    13021 ccgcttgcgg gtaatggata tccgccatcc cgcgaatatc catattgatt gtccccacca
    13081 ctacgcagta ttcctgctcg gtgagaatgt aacctttgtc tttaatccgg cctttgcgca
    13141 ttaaatccat aatatgcgcc gcaacgcgcg aacgactgat ttgcagcatg tccgcaattt
    13201 cgttctgctg aatcagcggg ttacgccgta aaattgcaag gatctccttt tcccggttat
    13261 ttatcttcag gctaccgctt tttcagtttg ttgcgctttc agccaggcga tctcttctgc
    13321 ccagatatcc gggttgatgg tttcaaggat cagcggaatg ccgtcgaaac ggtcgttctg
    13381 cataatccag cggaacgcat catgaccgat attgccttca ccgaggctat gatggcggtc
    13441 aacgcggctg ccaaaggtac ttttcgcatc gttaaggtgc atcccgcgca aatacttaaa
    13501 gccgacaata cgggcaaaat cagcgaacgt tttttcgcat tcggctggag tacgcaaatc
    13561 atacccggca gcgaaagcat ggcaggtatc aatgcagacg ccgacgcggg atttatcttc
    13621 cacgccgtcg ataatcgccg cgagatgttc gaatttaaac cctaagttac tgccctgacc
    13681 ggcggtgttt tcaatcaccg ctgttacacc ttgagtttta tccagcgcaa tgttgatgga
    13741 ttcggcaata cgcgcaaggc aatcctcttc tgaaatctgc atcagatggc tgccagggtg
    13801 gaagttgagc aaagaaagcc ccagttgttc gcaacgctgc atttcatcta taaaggcatc
    13861 gcgcgatttt tccagagctt cagtgaccgg atgtccgagg ttgatcagat aactgtcgtg
    13921 gggaagaatt tgcgccgatg tgtagtgata tttttcacag gcggctttga attcatcgat
    13981 ggtttgcgtc gtgagcggtg cggcacgcca ctgacgttgg tttttggtga acaaggcaaa
    14041 cgcggttgcg tcgatttcgg cggcgcgaat tgcggcattt gccagaccgc cagcagcact
    14101 aacgtgcgct ccaatgtatt tcatgcgagg actcctgtta aacccgctgg aggaaaacgg
    14161 taatgatagc gggttaacag gagcaagaag tagtggttta tgcgatgacg ctttgaatca
    14221 catagttaat cgcaccacca ccaacaatca gccaggcaaa cagtaccagt gccatcagca
    14281 gaggtttcgc cccagctttt ttcagcgcgc tgacgtgagt ggtcagaccc agcgccgcca
    14341 tcgccattgc cagcaggaag gtatccagcg ttaccagcat gttcaccacg ctctgcggta
    14401 acaggtggaa cgagttaaag atggcaacta cgatgaacaa gatggcaaac cacggaatag
    14461 tgattttgct tttctcgccg ctgttcgccc cagacagctg tttaacacgc gccgccagca
    14521 ggatgaggaa cggagccagc atcatcacgc gcagcatttt ggaaataact gctgcgtttt
    14581 ctgcatccgg gctgatggca tgacctgccg ccaccacctg cgccacttcg tgcacagtag
    14641 aaccaatgta gataccgaaa gtttccggac taaaccattg agacatcagc ggatatatcg
    14701 ccgggtagag gaaaatcgcg acggtcccga agataacaac ggttgcaaca gccacggtta
    14761 ctttactggc ttccgctttc actaccggct cagtcgccag taccgcggca gcaccacaga
    14821 tactgctacc ggcaccgatc aaccaactgg tgtgcttatc cagaccaaac actttctgcc
    14881 ccaggaagca agccagcagg aaggtactgg acagcgtcag cacgtcaatg atgatcccac
    14941 tgataccgac atcggcaatt tgcgagaacg tcagacggaa gccataaaga atgataccca
    15001 gacgtaataa atattgcttg gcaaacagca caccaccgtc acagcttttc cagatgtgcg
    15061 gatagatggt gttgcctaaa accatcccca acaagattgc gagggtgagg gcactaaacc
    15121 cggcacccgc aaccgcggga atggaaccac cccacagggc gaccccggtg ataactgcac
    15181 tcagggctaa ccccggaata aaatgccaca gtgtacgatg ttgtttctgt aaggtgatat
    15241 tcgtcataac cctctccttt aaccgggcat aaggttacga ctaactggtt taaaaataaa
    15301 attgattata tatttataat taatctttat aagtggtaag cgactatgca catcaccctc
    15361 cggcagttgg aagtttttgc agaagtattg aaaagtggat caaccaccca ggcgtcggtg
    15421 atgctggcgt tgtcgcaatc agcagtgagc gcagccttga ctgacctgga agggcagctt
    15481 ggcgttcaac tgtttgatcg cgtggggaaa agactggttg ttaatgaaca cgggcggctg
    15541 ctctatccgc gagcgttggc actgcttgaa caggcggttg aaatcgaaca actgtttcgc
    15601 gaagacaacg gcgcgattcg tatctatgcc agtagtacca tcggtaacta cattctgcct
    15661 gcagttatcg cccgttatcg ccatgattat ccgcagttgc cgattgaact tagcgttggg
    15721 aacagccagg acgtgatgca agcggtgctg gatttccgcg ttgatattgg ctttattgaa
    15781 ggaccgtgcc acagcactga aatcatttct gaaccgtggc tggaagacga gctggtggtt
    15841 ttcgccgcgc cgacttcgcc gttggctcgt ggtccggtca ccttagaaca gctggccgct
    15901 gcgccgtgga tcctgcgtga acgcggttcc ggcacgcggg atattgtcga ttatctgttg
    15961 ctgtcacatt taccgaagtt tgagatggcg atggaattag gtaactccga ggcaatcaaa
    16021 catgcggtgc gtcatgggtt gggaattagt tgcctgtcgc gacgtgtgat tgaagatcaa
    16081 ttgcaggcag gcacattaag tgaagttgcg gtccctctgc cgcgcctgat gcgtacgttg
    16141 tggcgtatac atcatcggca aaaacacctt tccaacgcgc tacggcgctt tctggactat
    16201 tgcgatcccg caaatgtgcc gcgttaagtt gctgtacaag aacatgctgg tgctgtgtcg
    16261 atttcgtgac gcagcgcctt cagcatgcat tcgccagaaa agagattggc tgctttactt
    16321 ataatccctg ggcgatcatg aaggtgtctt ataaccgtgt atttctgccg gaaggattgc
    16381 caatcgtctg ctacaatcgc gccatcattt ttaagatgga tagcattttt gtatgggttc
    16441 cgaaactaaa actacagaag cgccgggctt acgccgtgaa ttaaaggcgc gtcacctgac
    16501 gatgattgcc attggcggtt ccatcggtac aggtcttttt gttgcctctg gcgcaacgat
    16561 ttctcaggca ggtccgggcg gggcattgct ctcgtatatg ctgattggcc tgatggttta
    16621 cttcctgatg accagtctcg gtgaattggc tgcatatatg ccggtttccg gttcgtttgc
    16681 cacttacggt cagaactatg ttgaagaagg ctttggcttc gcgctgggct ggaactactg
    16741 gtacaactgg gcggtgacta ttgccgttga cctggttgca gcacagctgg tcatgagctg
    16801 gtggttcccg gatacaccgg gctggatctg gagtgcgttg ttcctcggcg ttatcttcct
    16861 gttgaactac atctctgttc gcggctttgg tgaagcggaa tactggttct cactgatcaa
    16921 agtcacgaca gttattgtct ttatcatcgt tggtgtgctg atgattatcg gtatcttcaa
    16981 aggcgcgcag cctgcgggct ggagcaactg gacaatcggc gaagcgccgt ttgctggtgg
    17041 ttttgcggcg atgatcggcg tagctatgat tgtcggcttc tctttccagg gaaccgagct
    17101 gatcggtatt gctgcaggcg agtccgaaga tccggcgaaa aacattccac gcgcggtacg
    17161 tcaggtattc tggcgaatcc tgttgttcta tgtgttcgcg atcctgatta tcagcctgat
    17221 tattccgtat accgatccga gcctgctgcg taacgatgtt aaagacatca gcgtcagtcc
    17281 gttcaccctg gtgttccagc acgcgggtct gctctctgcg gcggcggtga tgaacgcagt
    17341 tattctgacg gcggtgctgt cagcaggtaa ctccggtatg tatgcgtcta ctcgtatgct
    17401 gtacaccctg gcgtgtgacg gtaaagcgcc gcgcattttc gctaaattgt cgcgtggtgg
    17461 cgtgccgcgt aatgcgctgt atgcgacgac ggtgattgcc ggtttgtgct tcctgacctc
    17521 catgtttggc aaccagacgg tatacctgtg gctgctgaac acctccggga tgacgggttt
    17581 tatcgcctgg ctggggattg ccattagcca ctatcgcttc cgtcgcggtt acgtattgca
    17641 gggacacgac attaacgatc tgccgtaccg ttcaggtttc ttcccactgg ggccgatctt
    17701 cgcattcatt ctgtgtctga ttatcacttt gggccagaac tacgaagcgt tcctgaaaga
    17761 taccattgac tggggcggcg tagcggcaac gtatattggt atcccgctgt tcctgattat
    17821 ttggttcggc tacaagctga ttaaaggaac tcacttcgta cgctacagcg aaatgaagtt
    17881 cccgcagaac gataagaaat aagtttcctc ccttccttgc taagccctct caaccgagag
    17941 ggctttttca attccatttc cctgacaaat catgcggata taaaatttaa catttggatt
    18001 gataattgtt atcgtttgca ttatcgttac gacgcaatca aaaaaggctg acaaatcaga
    18061 ggctgttccg gctttctggg atgatcacct gcatacaaat aagtccaccg cgatgctgcc
    18121 gtacgcaagg ggacgtaaag aagatgtgag cgatacccat tttattttcg tagttacctc
    18181 atggagatat ggaatgttta ggttgaaccc tttcgtacgg gtcgggctgt gtttgtccgc
    18241 tatttcttgt gcatggcctg tgttagcggt cgatgatgat ggcgaaacga tggttgtcac
    18301 tgcatcttcc gtggaacaaa atcttaaaga tgcacccgcc agtatcagcg tcattaccca
    18361 ggaagacctg cagcgaaaac cggtacagaa tctgaaggat gtcctcaaag aagtgcctgg
    18421 cgtacaactg acgaacgaag gggataaccg taagggcgtt agtattcgtg gtctggacag
    18481 cagctacacc ctgattcttg tcgacggtaa acgcgttaac tcccgcaatg ccgtcttccg
    18541 ccacaatgat ttcgatctga actggatccc ggtcgattcc atcgaacgta ttgaagtggt
    18601 tcgtggcccg atgtcgtcgc tatacggctc cgatgcgctc ggcggtgtag tgaatatcat
    18661 caccaaaaaa atcggtcaga aatggtcggg taccgttacc gtcgatacca ccattcagga
    18721 acatcgcgat cgcggtgata cctataacgg tcagttcttt accagtggac cattaattga
    18781 tggtgtgctg ggaatgaaag cttacggcag cctggcaaaa cgtgaaaagg atgacccgca
    18841 aaactcaacg accaccgata ccggagaaac gccgcgtatt gaaggattct ccagccgcga
    18901 cggcaatgtc gaatttgcct agacaccgaa tcaaaatcac gattttactg ccggatacgg
    18961 tttcgaccgt caggatcgtg attccgattc gctggacaaa aaccgcctgg aacgccagaa
    19021 ctactgcgtc agccataatg ggcgttggga ttacggcacc agcgaactga aatcctacgg
    19081 tgagaaagtc gagaacaaaa accctggcaa cagcagcccg ataacttccg aaagcaatac
    19141 ggtcgacggc aaatacacgt tgccgctgac ggcgattaat cagtttctca cggttggcgg
    19201 tgaatggcgt cacgacaaac ttagcgatgc ggtgaacctg accgggggaa ccagctccaa
    19261 aacgtctgcc agccagtacg cgctgtttgt ggaagatgaa tggcggatct tcgagccgct
    19321 ggcgctgacg accggcgtgc gtatggacga tcacgaaacc tacggtgaac actggagtcc
    19381 gcgtgcctac atggtttata acgccaccga caccgtaacg gtgaaagggg gctgggcgac
    19441 ggcatttaaa gcaccttctc tgttgcaact tagccctgac tggacgagca attcctgccg
    19501 tggcgcatgt aagattgtgg gtagcccgga tctgaaacca gaaaccagcg aaagttggga
    19561 gctggggctt tactacatgg gtgaagaagg ctggctggaa gggggtgaat ccagcgttat
    19621 cgttttccgt aacgatgtga aagatcgtat cagcatcagc cgtacgtctg acgtcaacgc
    19681 tgcaccgggc taccaaaact ttgttggttt tgagacgggc gctaacggac ggcgcatacc
    19741 ggtatttagc tactacagcg ttaacaaagc tcgtattcag ggcgtggaaa ccgaactgaa
    19801 aattccgttc aacgatgaat ggaaactgtc gatcaactac acctacaacg atggtcgtga
    19861 tgtcagcaac ggcgaaaaca aaccgctatc cgatctgccg ttccatactg ctaacggtac
    19921 gctggactgg aaaccgctgg cgctggaaga ctggtcattc tatgtttctg gtcactatac
    19981 cgggcagaaa cgcgccgaca gcgcgacggc taaaacaccg ggcggttata ccatctggaa
    20041 taccggcgcg gcctggcagg tgactaaaga cgtcaaactg cgcgcaggcg tgctgaacct
    20101 tggcgacaag gatctcagtc gtgacgacta cagctataac gaagacggac gtcgttactt
    20161 tatggcagtg gattatcgct tctgatgaga agatgcccgg cgaaccgggc ggactttcac
    20221 ttcagtaaat actgcgcatg gaagcgcagg tgatcctcta taaaagaggc gatgaagtag
    20281 taactgtgat catatcccgg ctgaatacgc agcgtcatcg gccacgcttt ctggcgcgcg
    20341 gcttctgcca ataccgcagg ttgcaactgg tcggcaagaa actgatcatt atcgccctga
    20401 tcgataagcg tcgggatcgc atcctgcgcg ttactggcat acatcagcgc gcaactatcc
    20461 cattccagca tgcatttttg tcctcaccta aataggtgct aaacgctttg attccccacg
    20521 ggacgctgca cggattcaca atgggcgcaa aggccgaaac gctggtgtat ttacccggat
    20581 ttttcagcgc cataatcagc gcaccgtgac cacccattga gtgaccgcta atggcgcagc
    20641 ggtcgctgac attaaattgc gactgaacca gcgccggtaa ttcatcgcgc agataatcat
    20701 acatccggta atgcgtcgcc cacggcggtt gcgtggcatt aagataaaag cctgcgccct
    20761 ggcctaaatc gtagccatcg tcgttggcaa ccttttcgcc gcgcgggctg gtgtctggca
    20821 tcaccagtac aatccccagt tccgccgcta cccgctgggc acccgccttg gtggtgaagt
    20881 tctcgtcatt gcaggttaat ccggaaagcc agtacagcac tggtggcggg gtgtgatcac
    20941 gagggggggg gagaaagata ctgaacgtca tcgggcagtt taaggtactg gagtcgtgtc
    21001 gccagcgttg ctgccagcct tcaaaacagc ggtgctcttc gagcatttcc atgcgtggct
    21061 ccttgttgtg ttgtttgcaa tttcttcatc ataccgttta ttcaatgtgc tgtgagtaac
    21121 tttcacttcc gtatttgcat aacgatgttt taacatctgc tgatgaaagg cagcggcaat
    21181 tacaataatt atcgctgtga atactggatt atgtgcgccg cctcacgcac aataatcaag
    21241 ctgtaaatca gcttaataac tttgccccca cgcagggtgg aggcgtcaca cctgcaggag
    21301 aaatcataaa tgccatcact cagtaaagaa gcggccctgg ttcatgaagc gttagttgcg
    21361 agaggactgg aaacaccgct gcgcccgccc gtgcatgaaa tggataacga aacgcgcaaa
    21421 agccttattg ctggtcatat gaccgaaatc atgcagctgc tgaatctcga cctggctgat
    21481 gacagtttga tggaaacgcc gcatcgcatc gctaaaatgt atgtcgatga aattttctcc
    21541 ggtctggatt acgccaattt cccgaaaatc accctcattg aaaacaaaat gaaggtcgat
    21601 gaaatggtca ccgtgcgcga tatcactctg accagtacct gtgaacacca ttttgttacc
    21661 atcgatggca aagcgacggt ggcttatatc ccgaaagatt cggtgatcgg tctgtcaaaa
    21721 attaaccgta tcgtgcagtt ctttgcccag cgtccgcagg tgcaggaacg tctgacgcag
    21781 caaattctta ttgcgctaca aacgctgctg ggcaccaata acgtggctgt ctcgatcgac
    21841 gcggtgcatt actgcgtgaa ggcgcgtggc atccgcgatg caaccagtgc cacgacaacg
    21901 acctctcttg gtggattgtt caaatccagt cagaatacgc gccacgagtt tctgcgcgct
    21961 gtgcgccatc acaactgatt aaaaggcagg aaccatggag cgcaacgtca cgctcgattt
    22021 tgttcgcggc gtcgccattc tggggatcct gctattaaac atcagcgcct ttgggctacc
    22081 aaaggcggct tatctcaatc ccgcctggta cgatgctatt acgccgcagg atgcatggac
    22141 ctgggcattt ctcgatctca tcggccaggt gaaattcctc acgctttttg cgctgctgtt
    22201 tggtgctggc ctgcaaatgt tgctgccccg tggcagacgc tggatccagt cgcggttaac
    22261 gctgttagtc ttgttgggct ttattcacgg tttattgttc tgggacggcg atattctgct
    22321 ggcttacggg ctggtggggc ttggcgtttt gctgttattg gggctgattt ccgacagcca
    22381 gaccagccgc gcctggacgc cggatgcatc ggctattttg tatgaaaaat actggaagct
    22441 tcacggcggc gttgaagcga tcagtaatcg tgccgatggt gttggcaaca gtttactggc
    22501 actgggcgca cagtatggct ggcaactggc agggatgatg ctcattggtg cggcgctgat
    22561 gcgcagcggc tggctgaaag ggcagttcag cttacgtcac tatcgtcgta ctggttttgt
    22621 gctggtggcg attggggtga ccattaacct tcctgccatc gccctgcaat ggcagctgga
    22681 ctgggcgtat cgctggtgtg ccttcttact tcaaatgccg cgggaactga gtgcgccgtt
    22741 tcaggcgatt ggctatgcgt cgctgtttta tggcttctgg ccgcaattga gccgctttaa
    22801 gctggtgctt gcgatcgcct gcgtcggacg gatggcgctg accaactatc tattgcaaac
    22861 gctgatttgt accacgcttt tttaccacct cggtttgttt atgcattttg accgcctgga
    22921 gctgctggcg tttgttattc cggtatggct ggcgaatatc ctcttctctg ttatctggct
    22981 gcgttacttc cgccaggggc cggtggaatg gctctggcgt cagttaactt tgcgtgctgc
    23041 cggaccggca atatctaaaa catcaagata acgatctgga ttacattcgt taacaaaacg
    23101 gctgtaaccg tttccattgc tgtgactcga ttcacgaagt cctgtattca gtgctgacaa
    23161 aatagccgcc agcaagcagt catttactgc aatctcataa caggtagtga atatgatcac
    23221 cattcgtgat gtagcgcgtc aggctggcgt ctctgtggca acggtttccc gggtgctcaa
    23281 taacagcacg ctggtcagtg ccgacacgcg tgaagcagta atgaaagccg tgagtgagct
    23341 ggattatcgg ccaaacgcca atgcccaggc gctggcaact caggttagcg acaccattgg
    23401 cgtggtggtg atggacgttt ctgatgcgtt tttcggcgcg ctggtaaaag cggtggatct
    23461 ggtcgcccag cagcatcaga aatacgtgct aatcggcaat agctatcatg aagcggaaaa
    23521 agagcgtcac gccattgagg tgttaattcg ccagcgttgt aatgcgttga ttgttcactc
    23581 aaaagcattg agtgacgatg aactggcgca atttatggat aacattcccg gtatggtgtt
    23641 aatcaaccgc gttgtgccgg ggtacgccca tcgttgcgtt tgcctggata atctcagcgg
    23701 tgcccgaatg gcgacgcgca tgttgctgaa taacggtcat caacgtattg gttatctttc
    23761 ttccagccac ggcattgaag atgacgccat gcgtaaagca ggctggatga gtgcgttgaa
    23821 agagcaggat attattccgc cggaaagctg gattggcact ggtacgccgg acatgccggg
    23881 cggtgaggcg gcgatggttg aactgctggg gcgcaatcta caacttaccg ctgtatttgc
    23941 ttataacgac aatatggccg ctggcgcact gacagcatta aaagataatg gcattgcgat
    24001 tccgttacat ctctcaatca tcggtttcga tgatattccc atcgcccgtt acaccgaccc
    24061 gcaattaacg accgtgcgtt atcccattgc ttcaatggct aaattagcca ccgaactggc
    24121 cttgcagggg gcagcaggca atattgatcc tcgtgccagc cactgtttta tgccgacgtt
    24181 agtgcgtcgc cattctgtcg caacgcgcca gaatgcggcg gcgatcacta actcaacaaa
    24241 tcaggcgatg taaccgcttt caatctgtga gtgatttcac agtatcttaa caatgtgata
    24301 gctatgattg caccgtttta atgttgtaac ccgtatgtaa cagtgaataa tcactttcac
    24361 cgaggtaata gcgtcataac aacaattaaa gccgttttct ggagcgttac cgggcatgga
    24421 agaacgaatt ttaaaaagtg agcttcggcg ttcagtaaca cttcattaac tctactgccc
    24481 cgccgagcat ttatctcaag cactaccctg cataagaaaa accggagata ccatgaataa
    24541 gaaggtgtta accctgtctg ctgtgatggc cagcatgtta ttcggtgccg ctgcacacgc
    24601 tgctgatact cgcattggtg taacaatcta taagtacgac gataacttta tgtctgtagt
    24661 gcgcaaggct attgagcaag atgcgaaagc cgcgccagat gttcagctgc tgatgaatga
    24721 ttctcagaat gaccagtcca agcagaacga tcagatcgac gtattgctgg cgaaaggggt
    24781 gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg ggtacggtga ttgagaaagc
    24841 gcgtgggcaa aacgtgccgg tggttttctt caacaaagaa ccgtctcgta aggcgctgga
    24901 tagctacgac aaagcctact acgttggcac tgactccaaa gagtccggca ttattcaggg
    24961 cgatttgatt gctaaacact gggcggcgaa tcagggttgg gatctgaaca aagacggtca
    25021 gattcagttc gtactgctga aaggtgaacc gggccatccg gatgcagaag cacgtaccac
    25081 ttacgtgatt aaagaattga acgataaagg catcaaaact gaacagttac agttagatac
    25141 cgcaatgtgg gacaccgctc aggcgaaaga taagatggac gcctggctgt ctggcccgaa
    25201 cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg atggcaatgg gcgcggtaga
    25261 agcactgaaa gcacacaaca agtccagcat tccggtgttt ggcgtcgatg cgctgccaga
    25321 agcgctggcg ctggtgaaat ccggtgcact ggcgggcaca gtactgaacg atgctaacaa
    25381 ccaggcgaaa gcgacctttg atctggcgaa aaacctggcc gatggcaaag gtgcggctga
    25441 tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta ccttatgttg gcgtagataa
    25501 agacaacttg gctgaattca gcaagaaata agactgattg ggcgcagtcg tttactgcgc
    25561 ccttattgta tggccaacaa ggtaaaatta tatggtcagc tcaacgactc cgtcttccgg
    25621 ggaatacttg ttggaaatga gcggtatcaa caagtccttt cctggtgtta aggcacttga
    25681 taacgttaat ttaaaagtcc ggccacattc tatccatgca ttaatggggg aaaacggtgc
    25741 aggaaaatcg acattattaa aatgcctgtt tggtatttat caaaaagact ccggcaccat
    25801 tttattccag ggtaaagaga tcgatttcca ttccgccaaa gaggcactgg aaaatggtat
    25861 ttcgatggta caccaggagt taaacctggt attacaacgt tcggtgatgg ataacatgtg
    25921 gctggggcga tatcccacca aaggcatgtt tgtcgatcag gacaaaatgt accgcgaaac
    25981 caaagcgatt tttgatgaac tggatattga tatcgatccg cgtgcgcgcg tcggcacatt
    26041 atccgtttcg caaatgcaga tgatcgaaat cgccaaagcg ttttcctata acgcgaaaat
    26101 tgtgattatg gatgaaccga cttcttcgtt aaccgaaaaa gaggtcaatc atctgttcac
    26161 tattattcgt aaattaaaag agcgcggctg cggtattgtt tatatctcgc ataaaatgga
    26221 agaaatcttc cagttatgtg atgaagttac cgtattgcgc gacggtcagt ggatcgccac
    26281 cgaaccgctg gcaggactga cgatggacaa gatcatcgcc atgatggttg ggcgttctct
    26341 taatcagcgt tttcctgaca aagaaaacaa gccgggcgaa gtcatcctcg aggtgcgtaa
    26401 cctaacgtca ctgcgccagc cgtcgattcg cgatgtctcg tttgatctgc ataaagggga
    26461 gatcctcggt attgccgggc tggtgggggc gaaacgtacc gatattgttg agacgttatt
    26521 tggtattcgc gagaaatcgg ctggcaccat tacgttgcac ggcaaacaga tcaataacca
    26581 taatgccaac gaagccataa accacggatt tgcactggta actgaggagc gccgctcaac
    26641 gggaatttat gcctatctgg atattggttt taactcgtta atttccaata ttcgcaacta
    26701 caaaaataaa gttggtttac tggataactc gcggatgaaa agcgataccc agtgggtgat
    26761 tgattcgatg cgggtaaaaa cgccgggtca tcggacgcaa attggttcgc tctccggtgg
    26821 taatcagcaa aaggtgatta ttggtcgctg gctactaacg caaccagaaa tattaatgct
    26881 cgatgaaccg acgcgcggta ttgatgtcgg ggcgaagttt gaaatttatc agttaattgc
    26941 cgaactggcg aagaaaggca aggggattat tattatctcc tctgaaatgc ctgagttgtt
    27001 agggataaca gaccgtattc tggtcatgag caatggtctc gtttccggaa ttgtcgatac
    27061 aaaaacaaca acgcaaaacg aaattctgcg tcttgcgtct ttgcaccttt aagattaggg
    27121 gcttcccatg agtgcgttaa ataagaaaag ttttcttact tacctgaaag agggcggtat
    27181 ttacgtcgtt cttttagttt tgctggcgat tattattttc caggacccaa catttttaag
    27241 tctgttgaac ttaagtaata ttctcaccca gtcatcggtg cgtattatta tcgcgctcgg
    27301 tgtggcaggg ttaattgtca cccaggggac cgatctttct gctggtcgtc aggtagggct
    27361 ggcggcagtg gtggctgcga cattattgca gtccatggat aacgccaaca aagtgttccc
    27421 ggaaatggcg acgatgccga ttgcgctggt tattctgatt gtctgtgcca ttggtgcggt
    27481 gatcggtttg atcaacggtc tgattatcgc ttatctcaac gtgacgccgt tcattaccac
    27541 gctcggcacg atgatcatcg tctatggcat caactcgctc tattacgact ttgtcggggc
    27601 gtcgccaatt tctggttttg acagtggctt ctctaccttt gctcagggct ttgtcgcgct
    27661 ggggagtttc cgtctctctt acatcacctt ctacgcgttg attgcggtgg cgttcgtctg
    27721 ggtgttgtgg aacaaaaccc gcttcggtaa gaacattttt gccattggcg gtaacccgga
    27781 agcggcaaaa atatctggtg tcaacgtcgg cctgaacctg ctgatgatct acgcgttgtc
    27841 tggcgtgttc tatgcctttg gcgggatgtt agaagccgga cgtatcggct ctgccaccaa
    27901 caacctcggc tttatgtatg agctggatgc tatcgcggcg tgcgtggtag gcggtgtatc
    27961 gttcagcggc ggtgtgggga cggtgattgg cgtggtgacc ggggtaatta tttttaccgt
    28021 catcaactat ggcctgacgt atatcggcgt aaacccatac tggcagtaca tcatcaaagg
    28081 ggcgattatt atcttcgccg tagcgctgga ttcactgaaa tacgcgcgta agaagtgatg
    28141 tgattgcgga tattgccgga tgcgtctctg gttttacaac gttaccgggt gttctttctc
    28201 gcctttctta aacttcactt ccccgagctc aatacaaccc accgggcaga cgtgaccaca
    28261 aagcagacaa cccacacatt tctcggtatt acaatgcggt gtgcgggttt tctcgctcca
    28321 ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa cagcgtccac agccaacaca
    28381 tttgtcaaga ttgatacggg gatagacaat ataactgcgg tctaaatctt ccgccgggac
    28441 aatattgtta ttcgccagac ctaccatctc ctgcaacgaa tcaaatccct gatcggcgag
    28501 ataatgcgat aacccgctcg ccatatcttc tacgatgcga tagccgtact gcataatgcc
    28561 ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac tccgccgcat cttcccaggt
    28621 ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt tctggatggg tgcgcatttg
    28681 ctgaatgaag cgcagggcga tcggttttac cgctttaccg gaatatccgg aaatactcga
    28741 tttaccgtta acgatcggca taccgatttt ctgattaaga tcgatattgg tgatggattt
    28801 aacggtgtta attgccgcaa tgccatctgc gccgccgcgc ttcgccgcca gcgccacttc
    28861 gcacatatca ccgatattcg gcgtcatctt cgccagcatt ggcagcgtgg aaccccgttt
    28921 cactgcccga caatattttt ctaccagctc cgggctttgc ccgacatcgc tacccatcgc
    28981 atgagaagtc atttgcggac aggagaagtt acactcgatc atatccgcgc cagctgctcc
    29041 cattgctgct cattttcccc catgatcgaa gcgatcaata ctttgtccgg gtaatcttcc
    29101 ttcagccgac gcagggcggt cagattctct tccaacggat gttcagcaat ctgctccata
    29161 tttttgaagc cgataaaacc ggtatcttct ttcaccagat gatcaaaacg cggcgagact
    29221 tcgttggcga taaaaaagcc gatcgtttta aacaccacac cgccccaacc tgtgtcgtag
    29281 gctttggcac acatctcata gcagttgcct accggcgaag aagagaggca gaacgggttg
    29341 ggaaagttca cgccgcaaaa agtaatcgaa agatctttcg ttaacatgag caagctccct
    29401 ctaaatagtg atgaatcgcc tcggcggctt ctttcccggt tttcacggca tagaccacgg
    29461 ttttgtcacc ctcaacaata tcgccagcag caaagacttg cggatcgcgg atctgataat
    29521 tttgtgtttt gatggtgtta cgctgctgct ctaactccgc aaaggcatcc agtctggcat
    29581 gctgaccgac ggcgagaata attttatctg ccgccatcgt cagttcgcct gataaccgta
    29641 catgcttaaa cgtgacttta ttgccttcca cggctactgg cgtgaatcca tcgatgatcg
    29701 aaacgcccag ctcccgggcg ctggcaaact ctttttcgct tgccggaaac tcatctaact
    29761 cttcacgtgc tacgcaagtt accgcctgac agccgagaac tttcagcgtg ctggctacgt
    29821 ccatcgcgac atcaccgccg ccgataatta atgcgctttg tggaatgctg atatcgcctt
    29881 gcgcctgtcg tacacgttgc aggaagtcga cggcaatctc aacgtcgcta tgctcaaaca
    29941 gcggtagccc ggaaccgctt gataacccca cggtgaccag taccgcgcgg ttttccgctt
    30001 tcagttgctc cagggtgagt gttttaccga tttcgttatt gcacttaatg gtcacgccca
    30061 ttttttcaat acgggcgatc tctgcatcca gcactgattg cggtaaccgg aattgcggaa
    30121 taccgttacg caaccagcca ccggggtgcg cttctttctc ataaatcgtg acgtcataac
    30181 cctggtttgt cagtgtcaca ctggcctgca atccggcagg acctgcgcca ataatcgcga
    30241 ctttgccgag cgttttagta ccgggctgat aaatttccat cccggtttgt tgttcaaaat
    30301 cagtaacaaa acgctgtaag cggccgatat caatgggagc atcaacaccg gcacgggtac
    30361 aaccgctttg gcataatttc tccgtcgggc acactctggc acaaacggca ccgagggcat
    30421 tattttcgcg aattgtctcg gcagcgcctt taaaattacg aaagtagatt gagcgaataa
    30481 atttccccgg atcggtctgt gccgggcaag cctgactaca gggagcgtcg tgacataata
    30541 aacagcgaga ggcttcttta atcgccagta acggcgtaaa agccggagtg agttcatcca
    30601 gataattttg ttgcggcatg tgatcttcct taatgctatg agtaattatg gcgaacgcta
    30661 aataaatatt gcgtgctgat attaaggcta tgcgcgtggg gaaatttaag ctgtgaaagg
    30721 attcacaaat agctgcggtt cagaatgtta tatccgcagc ttattgagtg aagtaataat
    30781 tattctcaat aattaacaga aaagagaaaa tattaacgat agaaaataag ataaacaatc
    30841 atgccgatag ataaaattcc gaagccaata tatgccacca tataagcgaa gaagagtccg
    30901 ggattttttg ctcgccagac atacaccggt tccggtgcat ttttaacccg tttatcaatc
    30961 gcgcctgcgc gccagccttt ccatgcttcg cggaaacaaa atatcgggat aaggaaaaaa
    31021 acggcaacga caaaaaactg ctgtacatcc ataactactt tccttgtttc ttttgtaatt
    31081 caagtaactg ttcgggtgta acggcgggat acccctgcgc atcttccggc acctggtgca
    31141 tagccggaat agggaccagc ggccctaaaa aacgcggttc acgtttaaaa atataaaggt
    31201 cagccagcgc accgaaacgg gcggcaaatt cgcgaatacg tactgacagc atatctttcg
    31261 gtgacggtac ggcataacac tgagcctgaa tccccatatg cagcgcaata aataatgctc
    31321 gctcacagtg gaaacgttgg gtgataataa tgaaatcatt agtatcgaaa actttgcgtg
    31381 tacgcacgat ggaatccagc gtacgaaagc ctgcgtaatc gagaacaata tctgatgggt
    31441 cgacaccagc agcgattaaa tctttgcgca tggtcatcgg ctcgttataa ctttgcaatg
    31501 cgttatcgcc gctcagtaat agataattta ccttaccgct gttataggca ttaatcgctc
    31561 cttgaatgcg gtagcgataa tactgattaa taacgccggt acgataatat tttgctgtgc
    31621 cgagcaccac gccgacctgg cggtagggga gatcctgcaa ttcgtcgtag atataaggcg
    31681 cggttttcca gctcatccag cgatcgaggc cgagcacagt caacagcagc aagccgatca
    31741 ggactaacag gctgaggaac acgcgcttta acatgcagga gactcgagct aaggaaaatt
    31801 tccatcaggc tactttaccc gctggttttg cgcaagaaac ggcaaactgt ttgccgggaa
    31861 tttcgacacc aggagagggc tggcaagccg gacaaggcgt tcacgccgca tccggcacca
    31921 ggcttaagcg agaagcacgc ggtcgatact gtgacagccg agagctttca gcgttgcgga
    31981 ggtggcatcc cactgaatca acggcgcatc ggctttttct gccagaaccg cgcgctggat
    32041 atccgggtaa tcataaccct tgagattcaa cagaattaac gccccctgca acggcggcag
    32101 agtcgggttg aatgcggcgt tttcagcgta gctgccgctg aaaatacgac cgtctttaca
    32161 ttccagcgcg acaccgcttg gtgacttact gtaaggcatg tgcgaacggt ttgccgctgc
    32221 aatcgccgcc tgagaaagcg catcaccagt cagcgcatag ccgtgatcct gctcgtccat
    32281 cagcagcgtt ttaatctcca gatctttcgg cccaaaggca tccggcagat agtcacgcag
    32341 cgcgtgtgcc tcgcggcccg gcaaatgaat acgcagatcc agaccgctgt tcagttcatt
    32401 cataaactga cggcagtgac cacaaggcgt atagttaacg gtgatggctg caagcgcttt
    32461 ttcaccactc aaccaagcgt ggctgatcgc gctttgttcg gcatgaacgg tttgctgcat
    32521 tgttgcacca ataaattcca tattggcacc aaaataccag gttccgctca caccgcgcgc
    32581 aattgcgcca acattaaaat tcgacaatgg cgtacgcgca caggccgccg ccagcggaag
    32641 tagtgcgaat gccagcgcgt cttcgtccag tcccgttgcg ctcttcagcg atgagacttg
    32701 ctccccggtc aacaaagcgg ggaagtactt gtctgccaga ataggttcca gtgcagattg
    32761 caagttatcc gcaagttggg caaaagcggt ttgaaaacgt ggatgcatgg cgttgcctca
    32821 taatcatgta atgggataac agtttacgaa cccgttatgg ctttatatgt gatctgaatc
    32881 tcattaatta tgcaacacgt gaaacttaca tcaaaaatgc gcgacgtatc gcaaatttta
    32941 gcccattact gccagaataa tcgggaaaag gaacggtgcg atcagcgaag taattatccc
    33001 gcataacacc agcgctagcg aactaaatgc accttcctga taatccagct cggcgcagcg
    33061 cgccgtaccg agggcgtgcg aggcagtccc cattgccaga ccgcgcgcag ctttggtacg
    33121 aatacgcatc gcattaagca atgtatggcc aaataccgcg ccgaggatgc cgacgaaaat
    33181 cacgcaaact gcgctgattg ccggaatacc gccaatactg ccgccaaccg ccattgcaat
    33241 aggcgtggtg actgatttcg gcaggattga cgcggcgatt tccggtgaag cgcccatcaa
    33301 caatgccacg gaagtccccg tcaccatcgc aaccacgctg ccgataaaac agatggtgat
    33361 gatcgatttc cagcgcgcgc ggatctggtg tagctgctca tataaaggat aggccagcgc
    33421 gacgaccgcc ggttgcagca gatcgtttag cacttcgcta cctttaaagt aactgtcgta
    33481 agagatgccg gtcagcatta aaaaaggaat gatcaccacc attgctacca gcaacgggtt
    33541 aagcagggga aacttatacc gtgccgccag tttgcgggcg gcaaaaaaga caatcaacgt
    33601 cagcggtaat gaccaccaga tattcgccat cattcttctg atcctttctg acctacgact
    33661 ttacgttcac cgtgtaccag ttgtgaactc cagctcacca ccagaaaaac caccagcgta
    33721 ctgacagcac aggaaaccac taccgggcca aactgcgcgc ggagcaaatc aaaatattgc
    33781 atgacgccta cgccaatcgg cacaaacaat agcgccatat agcgaatcag tacgtagcac
    33841 cccggattga cccatttcgc cggaagaatt tgcaaggcca gcaggacaaa caggatcagc
    33901 atcccgatga tgctgcccgg aatggttacc ggtagcaggg aagcaatgaa aatgcctgca
    33961 tacaggcagg cataaatcag gacgaaagcg cgtaaatatt gccagataat gttcagtgtc
    34021 ttactcataa tcaacacccc agatgagatg gattcatcat acaattaatt cgtaaaacgt
    34081 gctaccgatc acattaagaa atcagcgctt attttttcgc tacccggtat tacgcaatga
    34141 gcagcgaact caggcataca tcaagcatag agagggattg cagtttgcag caatccctct
    34201 ttgcagacct ttaatccgct gaccataggc gctaacatag cgcctcattt ttttgcgggt
    34261 gatgatatgc gtgtattact ggcaccgatg gagggagtgc ttgactctct ggtgcgtgaa
    34321 ttgctgaccg aagttaacga ctacgatctg tgcatcaccg agtttgtccg cgtggtggat
    34381 caactgctgc cggtaaaagt ctttcatcgc atttgccctg agctacaaaa cgccagccgg
    34441 acaccatctg gtacgctggt gcgcgtgcag ttgttaggtc agttcccaca atggctggca
    34501 gagaacgccg cccgtgcggt ggagttaggt ttctggggcg tggatctcaa ttgcggctgc
    34561 ccgtcgaaaa cggttaacgg tagcggcggc ggggcgacgt tactcaaaga tcctgaactc
    34621 atctaccagg gggcaaaagc gatgcgtgaa gctgtaccgg cgcatttgcc tgtcagcgtg
    34681 aaagtgcgtc tgggctggga cagcggtgag aagaaatttg aaatcgccga tgcggttcaa
    34741 caggctggcg ctacggagct ggtggtgcat gggcggacga aagagcaggg ttaccgcgcg
    34801 gagcatattg actggcaggc gattggcgag attcgccagc ggctgaatat tccggtgatt
    34861 gccaacggtg aaatctggga ctggcagagc gcgcaacaat gcatggcgat cagcggctgc
    34921 gacgcagtga tgattggtcg cggggcgctc aatattccca acctgagccg ggtggtaaaa
    34981 tataacgaac cgcgaatgcc gtggccggag gtggttgctt tgctgcaaaa atatacccgt
    35041 ctggaaaagc agggcgatac cgggttatat cacgttgcgc ggattaaaca gtggttgagt
    35101 tatttgcgta aagaatacga tgaagcaacg gaattatttc agcatgttcg ggtgttgaat
    35161 aactcccctg atattgcaag ggctattcag gcaattgata tcgagaaact ctaaaaacca
    35221 tagtgatgtt tttgacaatg tgttagctct ttatcaatag gccgcacggt tgtttggtgg
    35281 cgtcaaaatc gccgaggcgt tccctgaagg ccggggcagc ccacatggat gtgggctgag
    35341 ggcgcgtttt acagggatgt tacctcgcgc ccgacccggt agccgtaagg gataagccga
    35401 gggcaccgcg cagcggcgat tttgttcgcc agagcccggg ggggcagggg gcggcggcga
    35461 ttggtcgccc cctgcgcgct ccttgcgcca gtgattatat gaagcaatgc acatgacagg
    35521 agcgcaacag gatgagagac tgaaatattc tcgttatttt cggatctgat gacattaatt
    35581 aattttaaaa tatcatctta aatacgccag tcaccaccag tagcccaatc aggaaaataa
    35641 tcaatacagc ccagagtata attttcattg gcgttccttt atttgtgagt gctgaataag
    35701 tataggaaaa aacgaagccc tttgccgtta tccataaacg attaagataa atcggaagat
    35761 atcatttact tagtctatat ttttctgggt atgtcgtttt attgctactg atgtattgcc
    35821 acgcgaaact aaaaaatata tcgcaacaca cggtaatctc cgcgcaccgt ttcttacctg
    35881 attattcccc catgaatcgt gattcctttt atccagccat cgcctgtttt ccgctgttac
    35941 tgatgctggc cgggtgtgcg cctatgcatg aaacccgcca ggcgttaagc cagcaaacgc
    36001 ccgctgcaca agttgacacc gcattaccca cggcgctgaa aaatggttgg ccagacagcc
    36061 aatggtggct ggagtatcac gataatcagc tcacttcatt aattaacaat gcactacaga
    36121 acgcaccgga tatgcaggtg gctgaacagc gcatttagct agctgaagcc caggcgaagg
    36181 cagttgccac tcaggatggt ccgcagatcg acttttcggc ggatatggag cggcaaaaaa
    36241 tgtcggcaga agggctaatg gggccgtttg ctctgaacga tccggccgca ggtacgaccg
    36301 gtccgtggta caccaacgga acttttggct taacggcggg ctggcatctc gatatctggg
    36361 gaaagaatcg ggcggaggtt actgcccgcc tgggtacggt taaagcacgg gcggcggaac
    36421 gcgagcaaac ccgccaattg ctggctggca gcgtagcccg cctgtactgg gagtggcaaa
    36481 cccaggcggc gttaaacacg gtcttgcagc aaatagaaaa agagcagaac accattatcg
    36541 cgaccgatcg ccagctatat cagaacggga ttacttcttc agttgacggt gtggaaaccg
    36601 atattaatgc cagcaaaacc cggcagcagc tcaacgatgt cgcggggaaa atgaaaatta
    36661 ttgaggcacg gttaaatgca ctcacaaatc accagactaa gtcattgaag cttaaaccgg
    36721 tcgcgttgcc gaaagtggca agccagcttc ctgatgaact ggggtactcc ttactggccc
    36781 ggcgggcaga tttgcaggcg gcgcactggt acgttgagtc atcgttaagc actattgatg
    36841 cggcaaaagc ggcattttat cctgacatca acctgatggc cttcctgcaa caggatgcgt
    36901 tgcacttaag cgatctgttc cgtcattccg cgcagcaaat gggcgttacg gcaggcctga
    36961 cgctacctat tttcgatagt ggtcgtctta acgccaatct cgatatcgca aaagccgaaa
    37021 gcaacttgtc tatcgccagc tacaacaaag cggtggttga agcagtgaat gacgtggcgc
    37081 gggcagccag tcaggttcag acactggcgg agaaaaacca gcatcaggcg caaattgagc
    37141 gcgatgcctt gcgtgtggta ggtcttgcgc aggcccgctt taacgcgggc atcattgctg
    37201 gttcccgcgt cagcgaagcc agaatcccgg cgctgcgtga gcgggccaat ggcctgttat
    37261 tgcaagggca gtggctggat gcctccattc gactcaccgg tgcgctgggc gggggttaca
    37321 aacgctgatg atatgcgcct tctatactta acgtttattc agcgttaagt ggagaactcg
    37381 atggcatagg tcgcgataat taccgcttcc gattcgggga tcggcaaaga gtgcgcgtta
    37441 ttactggcgc agcaggggtt tgatattggc attacctggc actcagatga agaaggggca
    37501 aaagataccg cgcgtgaggt ggttacccac ggcgtacgtg cggagatcgt gcaactggat
    37561 ctcggcaatc tgccagaagg tgcgcaggcg ctggagacac tcatccagcg atttgggcgt
    37621 attgatgtgc tggtgaataa tgcgggtgca atgaccaaag cgccgtttct tgatatggct
    37681 tttgatgagt ggcgcaagat ttttaccgtt gatgttgatg gtgcattttt atgctcgcaa
    37741 attgcggctc gtcagatggt gaaataaggg cagggcggtc gcatcatcaa cattacgtcg
    37801 gtacatgaac atacgccgct gccggatgcc agcgcctaca cagccgctaa acatgcgctc
    37861 ggcgggttaa ccaaaacgat ggcgctggag ttggtcaggc ataagatttt ggtgaacgca
    37921 gtcgcgcctg gggcgatagc cacgccaatg aacagcatgg atgatagcga cgtgaagccc
    37981 gacgcggagc cttcgattcc tctacggcgt tttggcgcaa cgcatgagat tgccagcctg
    38041 gtggtgtggc tttgttcgga gggcgcaaat tacacgacag ggcagtcgtt gatagtggat
    38101 ggcggcttta tgttggcgaa tccgcagttc aacccagaat agcgcagtga ggtgcgtgga
    38161 tatttcaatg ggttaggcca gataagacac cagcgtcgca tcaggcatgg aacacccggt
    38221 gccggatgtg gagtgaatgc ttatctgtgg caactgcttt acgcctgatt atccggcttt
    38281 ttcttcccgc gccgtttcag ccaccagcgc acgcccacca ccagaaccac gaccagaatc
    38341 aaccagaccc agtgcttcaa atgctggtcg agattgtgca accacggcgc aatcacctga
    38401 ccaccagcgt aaccaatagt ggtaaaaatc aacgcccagg caaatgcgcc gagaatattc
    38461 agcggcagaa agattttcgg cggcagctgg ctggcaccaa tcagcgtcgg gccaatcacc
    38521 cgaaagccat acataaagcg cgtaccaatg acaaacagat acggatggcg ttggataagt
    38581 ttctgtgccc gctcaatttt atcctgatgt ttcgagaaac ggcgtaacag cttgccgcca
    38641 aaccgccgcc cgcacagata gagcacctgg tcgccaatca tgccaccaag cgccacagaa
    38701 agtaccacca gtgggaactt taatagcccc tgatgtgccg caacgcctcc cagcaaagtc
    38761 acggtttcac cttccgccag gctaccgata accagcgcgg cataaccata ttgtgagatg
    38821 agtgtattga gatccatagg ccagtttgtc tccctttggt tgcctgtaag catacaccct
    38881 gtgaatcatt cacgctgaaa aaggacatac atcagacggt gcaaattaca tggatgaaca
    38941 aaaaataatc acaagctggc ttatacttga actgtcaggt tcatgaactc acaacaaggg
    39001 ggcggtatga gccatgtctg gggactgttt tcccatcccg atcgtgaaat gcaggtgatt
    39061 aatcgcgaaa acgaaacgat ttctcaccat tacacccacc acgttttgct gatggcggcg
    39121 attccggtga tttgcgcctt cattggcact acacagattg gctggaattt tggcgatggc
    39181 actatcctga agttatcctg gtttaccgga ctggcgctgg ctgtcttgtt ttacggcgtt
    39241 atgctggctg gggttgcggt gatggggcgg gtcatctggt ggatggcgcg caattacccg
    39301 cagcgtccgt ctcttgcgca ctgcatggtc ttcgcgggct acgttgcaac tccgctgttt
    39361 ttaagcggtc tggtggcgct ttatccactg gtctggttgt gtgcgctggt cggcacggtg
    39421 gcgctgtttt acaccggcta cctgctttac cttggtattc cgagcttcct gaatatcaac
    39481 aaagaggaag gccttagctt ctccagttcg acgctggcga ttggcgtgct ggtactggaa
    39541 gtgctgctca ccctaaccgt tatactatgg ggctacggtt atcgtttgtt ctagaaagtt
    39601 ctttgacgtt gcattgctgg cgtaaatgtc agcaatgcag catttactca cgattctcct
    39661 ttgacgatcg cactttttgc tcgttatgat ggcgtcgcta gcctcagtaa atccttactc
    39721 cggcggtgcg caacccgtgc gcgtgaacca ctatctgaat gctcatcatg ccgaaatttc
    39781 gagtttcttt atttagcttg gccctgatgc tggctgtgcc ttttgcaccg caggccgttg
    39841 cgaaaacggc agccgctacc accgcttcac aaccggaaat tgcctccggt agcgcgatga
    39901 ttgttgatct gaataccaac aaagtgatct attcgaacca cccggatctg gtgcgtccga
    39961 ttgcgtctat cagcaaatta atgaccgcga tggttgtgct ggatgcacga ctgccgctgg
    40021 atgaaaaact aaaagtggat atcagccaga cgccggagat gaaaggggtc tattcgcgcg
    40081 tacgactgaa tagcgaaatc agccgtaaag atatgctgtt gctggcgctg atgtcttcag
    40141 aaaaccgcgc ggcggcaagc cttgcgcacc attatcccgg tggttacaaa gcctttatta
    40201 aggcaatgaa tgcgaaagcg aaatcgctcg gaatgaacaa cacgcgcttt gttgaaccta
    40261 ccggattgtc ggtgcataac gtttcaactg cccgtgactt aaccaaactg ctcattgcca
    40321 gcaaacaata tccgttgatc gggcagttaa gtactacccg ggaagatatg gcaaccttct
    40381 ctaacccgac gtatacgctg ccgttccgca atactaatca tctggtgtat cgcgataact
    40441 ggaatattca gttaaccaaa accggcttta ccaatgcggc gggccattgt ctggtgatgc
    40501 gtacggttat caataataaa ccggtggcgc tggtagtgat ggacgcgttt ggcaaatata
    40561 cccattttgc cgatgccagc cgcctgcgta cctggattga aaccggtaaa gtgatgcctg
    40621 tgccggcagc agcgttgagc tataaaaaac aaaaagccgc ccaaatggcg gcggcggggc
    40681 agacggcaca gaacgattaa gtgaattcgg atggcgatac tctgccatcc gtaattttta
    40741 ctccacttcc tgccagtttt tccgtttact ggttttaccg atccccggat tcatgctatt
    40801 ggtcggatca ttctcgcgat agaacttctg caacgtctcc ggtgctttat acaaatgacc
    40861 gacgttatgc tcggcagggt actgcgcgcc gcgctgctgt agcagttcca gcatctgttc
    40921 ttttaacgca tgcacatcca cgcctttttt cactatgtaa tcctgatgga agacatagca
    40981 cataaaatgg ccgtaataga gcttgtgcac cagctggctg tcgatctccg gcggtaaatg
    41041 ctcataccac tcggtgtcgt tacgccgcag agcgatatcc agtgccagaa tgtcttcgac
    41101 ttcatcggaa tgcaccgcct gataacgaat tgctgcgccc gcagcggcga aacggtgtaa
    41161 aaacgctttg ctaccttcct ccggcgtaca gacaaagaaa tcgccctcgg cctgtttgaa
    41221 ataatccacc agccacgatt tggcttcacc gacgccatcg cctgccattt ttaacagcag
    41281 atgatgctcg tatttatcgc gccagttttt catgcgcggc ggtaaatggc tggggaacag
    41341 gtgaccgaat ttttgcattg cacggtcagt aaaatgcgga cggaagaatt tcactttctc
    41401 cagcatcgca tcggtgcgtc ccttgagatt aaagaagaac ggcatcttgt cggtgcctaa
    41461 cttatcaatc atcaggaagg tgtctttgcc gtatttttcc gcaatatcgt agatatcccg
    41521 gtgcatatat tccccggcaa ccggcagatt ttcgaagtta gccagaatat gacggcggat
    41581 ttcggtcagc acttccggct ggttggtgcc gatataaaac acctgctgat ttttttccgc
    41641 ttcgaaggta tcaagacgta ccgcaaagac ggccagcttc ccggcgcaac cagaagattc
    41701 aaataaccga tccggatcgg cgttataacg tgcgggcgtg tcggcttcaa tatcacgaac
    41761 gcggtggaca tagtcataat cgtgggcgtg acgaccatcg tgacgcacat cgtcatcttt
    41821 gatgcgatcg tcatccagct tgctaaggat ctgctccggc gtttcgccca gatcaatccc
    41881 cagatggttc accagcgtca gtttgccatc ttcatctata cgcgcgaata acgacatttc
    41941 ggtatacgcc gggccacgtt gcaccagcga gccgccggag ttgttacaaa taccgccgat
    42001 gaccgatgcg cctatacacg atgatccaat caccgaatgc ggttcgcgtc ccagcggttt
    42061 gagggctttt tccagcgaat agagcgtggt gcccggatag gccagcacct gttcgccctt
    42121 gccgagaacg tgcagcttgt cgagacgcag ggtgctgatg atcacgatat cgcgatcata
    42181 gtcgttaccg tttggcgtcg atccttcggt caggcctgta ttggcagcct gcatcagaat
    42241 aattttgtcg gcggtgacgc aggctttcag cacccgccac aattccagta gtgagccagg
    42301 gaaaacgaca gccagcgcgt cgccctgacc agaacggaag cccttgcgat agcgggccgt
    42361 ttttgcggga tcggtgagca ggtgtgaatg accgaccaga cgggcaagtt cattcaaaaa
    42421 ggctttatta tcagttgttg tcatggaaga cattttccac tccttgtggt ggcgaaaaaa
    42481 tatcacaaac tagaatagcg ccttgtatga caattcagcg agtgaatatt ttgaaaattc
    42541 agacaataaa ctgagcattc tttttctctc tccatcatgc ttatggcaaa ctacggtttt
    42601 tgcaccattt tcgctgccac gtcgggcaac aaaggaagaa aaatccatat gaaatggcta
    42661 tgttcagtag gaatcgcggt gagtctggtc ctgcagccag cactggcgga tgatttattc
    42721 ggtaaccatc cattaacgcc tgaagcgcgg gatgcgttcg tcaccgatct gctcaagaaa
    42781 atgacggttg atgagaaaat tggtcagctg cgcttaatca gcgtaggccc ggataatccg
    42841 aaagaggcga tccgcgagat gatcaaagac ggtcaggttg gggcgatttt caacaccgta
    42901 acccgtcagg atatccgcgc catgcaggat caggtgatgg aattaagccg cctgaaaatt
    42961 cctcttttct ttgcttacga cgtgctgcac ggtcagcgca cggtgttccc gattagcctc
    43021 ggtctggcct cgtcttttaa cctcgatgcg gtgaaaacgg tcgggcgtgt ctctgcttat
    43081 gaagcggcag atgatggcct gaatatgacc tgggcaccga tggtcgatgt ctcgcgcgat
    43141 ccgcgctggg gacgtgcttc cgaaggtttt ggcgaagata cgtatctcac ctcaataatg
    43201 ggtaaaacca tggtggaagc gatgcagggt aaaagcccgg cagatcgtta ctcggtgatg
    43261 accagcgtca aacactttgc cgcatacggc gcggtagaag gcggtaaaga gtacaacacc
    43321 gtcgatatga gtccgcagcg cctgtttaat gattatatgc cgccgtacaa agcggggctg
    43381 gacgcaggca gcggcgcggt gatggtggcg ctgaactcgc tgaacggcac gccagccacc
    43441 tccgattcct ggctgctgaa agatgttctg cgcgaccagt ggggctttaa aggcatcacc
    43501 gtttccgatc acggtgcaat caaagagctg attaaacatg gcacggcggc agacccggaa
    43561 gatgcggtgc gcgtggcgct gaaatccgga atcaacatga gtatgagcga cgagtactac
    43621 tcgaagtatc tgcctgggtt gattaaatcc ggcaaagtga ccatggcaga gctggacgat
    43681 gctgcccgcc atgtactgaa cgttaaatat gatatggggt tgtttaacga cccatacagc
    43741 catttggggc cgaaagagtc tgacccggtg gataccaatg ccgaaagccg cctgcaccgt
    43801 aaagaagcgc gtgaagtggc gcgcgaaagc ctggtgttgc tgaaaaaccg tctcgaaacg
    43861 ttaccgctga aaaaatcggc caccattgcg gtggttgggc cactggcgga cagtaaacgt
    43921 gacgtgatgg gcagctggtc cgcagccggt gttgccgatc aatccgtgac cgtactgacc
    43981 gggattaaaa attccgtcgg tgaaaacggt aaagtgctgt atgccaaagg ggcgaacgtt
    44041 accagtgaca aaggcattat cgatttcctg aatcagtatg aagaagcggt caaagtcgat
    44101 ccgcgttcgc cgcaagagat gattgatgaa gcggtgcaga cggcgaaaca atctgatgtg
    44161 gtggtggctg tagtcggtga agcacagggg atggcgcacg aagcctccag ccggaccgat
    44221 atcactattc cgcaaggcca acgtgacttg attgcggcgc tgaaagccac cggtaaaccg
    44281 ctggtgctgg tgctgatgaa cgggcgtccg ctggcgctgg tgaaagaaga tcagcaggct
    44341 gatgcgattc tggaaacctg gtttgcgggg actgaaggcg gtaatgcaat tgccgatgta
    44401 ttgtttggcg attacaaccc gtccggcaag ctgccaattt ccttcccgcg ttctgtcggg
    44461 cagatcccgg tgtactacag ccatctgaac accggtcgtc cgtataatgc cgacaagccg
    44521 aacaaatata cttcgcgtta ttttgatgaa gctaacggag cgctttatcc gttcggctat
    44581 ggtctgagct ataccacttt caccgtctct gatgtgaaac tttctgcgcc gaccatgaag
    44641 cgtgacggca aagtgacggc cagcgtgcag gtgacgaaca ccggtaagcg cgagggggcc
    44701 acggtagtgc agatgtactt gcaggatgtg acggcttcca tgagtcgccc tgtgaaacag
    44761 ctgaaaggct ttgagaaaat caccctgaaa ccgggcgaaa ctcagactgt cagcttcccg
    44821 atcgatattg aggcgctgaa gttctggaat caacagatga aatatgacgc cgagcctggc
    44881 aagttcaatg tatttatcgg cactgattcc gcacgcgtta agaaaggcga gtttgagttg
    44941 ctgtaatgct ttagtaaggg cgcgacgttt gcgccctttg cctgatgttc atatcttaac
    45001 ttgctgaatt ttcgcccttt gtaggccgga taaggcgctc gcgccgcatc cggcaggaaa
    45061 cgccggaccg aatatgcctc ttaagtattt atctgattct gcaactgaat ccttccgctc
    45121 aggctaactc tgtcattatc aactatgctt ttctcttaat tcgctgaaaa caacagtaaa
    45181 agaatgagga aagcatcatg ccactcttaa agctctgggc aggttcactg gttatgttgg
    45241 cagccgtgag cctgccgcta caggcggctt cccccgttaa agtcggttca aaaatcgata
    45301 ccgaaggcgc gctgctcggc aacatcatct tgcaggtact cgaaagccac ggcgtaccga
    45361 cggtgaataa agtgcaactc ggaacgactc ctgtggggcg cggggcgatt acttccgatg
    45421 aactggatat ctatccggaa tataccggca atggcgcttt cttctttaaa gatgaaaacg
    45481 atgcagcgtg gaaaaatgcc gggcagggct acgagaaagt caaaaagctc gatgcggagc
    45541 acaacaagtt aatctggctg acgcccgcgc ctgcaaataa cacctggact atcgccgtgc
    45601 gtcaggatgt ggcagagaaa aacaaactca cttcgcttgc cgacctgagt cgttatctgc
    45661 aagagggcgg caccttcaaa ctggcagcct cggcagagtt tatcgaacgc gccgatgcgt
    45721 tacccacgtt tgaaaaagcc tatggcttta agctcggtca ggatcagttg ctgtcactgg
    45781 ctggtggcga cacggcggtg acgattaaag ccgctgccca gcaaacctct ggcgttaatg
    45841 ccgcaatggc ttacggcact gacggtccgg tcgcggcgct ggggctgcaa accttaagcg
    45901 atccgcaagg cgtgcaacct atctacgcgc ctgcaccagt ggtgcgtgaa tcggtgttga
    45961 aggagtatcc gcaaatggca cagtggctac agccagtctt cgccagcctc gatgcaaaaa
    46021 cattgcagca actgaatgcc agcattgcag tggaaggact ggatgccaaa aaagtggctg
    46081 ccgactacct gaaacaaaaa gggtgggcga agtaatttcc cgtgacttat ctacgtatta
    46141 atcctgttct ggcgctgctg ttgttgctga cggcaatcgc agcggcgctg ccgtttatca
    46201 gttacgcgcc taatcgttta gtctcgggtg aagggcttca tctctggcaa ctgtggccgc
    46261 aaacgatctg gatgctggtg ggcgttggtt gcgcctggct gacagcctgt tttgtccccg
    46321 ctaaaaaagg cagcattttt gcactcattc tggcgcaatt cgtcttcgta ttgttggtgt
    46381 ggggagctgg aaaggcggcg acccaactgg cgcaaaatgg cagtgcgctg gcgcgtacca
    46441 gcctcggcag aggtttctgg ctggctgcgg cgctggcatt gctggcctgt agcgatgcca
    46501 tccgccgaat ctccacgcat ccgctgtggc gctggttgtt gcatatgcag attgccatta
    46561 ttccgctgtg gttgctgtac tccggcacgc ttaacgatct ctcactaatg aaagaatacg
    46621 ccaaccgtca ggatgtgttt gacgacgcgc tggcacaaca tctgacgttg ctgtttggtg
    46681 cggtgctgcc tgcgttagtg attggtgtgc cgttgggcat ctggtgctac ttttccactg
    46741 cgcggcaggg ggcaattttt tctctgctca atgtcattca gaccgtgcct tcggtggcgc
    46801 tctttggcct gttgattgcg ccgcttgccg cgctggttac ggcctttccg tggctgggga
    46861 agctcggcat agcaggaacc ggaatgacac ccgcactgat tgcgctggtg ctctatgcct
    46921 tgctgccgct ggtgcgaggc gtggtagtcg gtttgaacca gatcccgcgc gatgtgctgg
    46981 agagcgccag agcgatgggg atgagcgggg cgcagcgttt cctgcatgtt cagttaccgc
    47041 tggcgttacc ggtatttctg cgcagcctgc gggtggtgat ggtgcaaact gtaggtatgg
    47101 cggtgattgc ggcgttaatc ggcgcaggcg gttttggtgc gctggttttc caggggctgc
    47161 taagcagcgc cattgattta gtgttgctgg gggtgatccc ggtaattgtt ctggcggtgc
    47221 ttaccgacgc gctgttcgat ttgcttatcg cactgctgaa ggtgaaacgt aatgattgaa
    47281 tttagccatg tcagcaaact gttcggcgca caaaaagccg ttaacgatct caatctcaat
    47341 tttcaggaag ggagtttttc ggtgctgatt ggcacatctg gctccggcaa atccaccacc
    47401 ctgaaaatga ttaaccgcct ggtggagcat gacagcggag tgatccgctt tgccggagaa
    47461 gaaattcgct cgctgccagt actggagttg cgccgccgga tgggctatgc cattcaatct
    47521 attggcctgt tcccccactg gagcgtggcg caaaacatcg ctaccgtgcc gcaattacaa
    47581 aaatggtcgc gggcgcggat tgacgatcgt atcgacgaat taatggcgct actggggctg
    47641 gagtcaaatt tgcgtgagcg ttatccgcat cagctttccg gtggtcagca gcaacgtgtg
    47701 ggagtggcgc gtgcactggc tgccgatccg caagtcttac tgatggatga accttttggc
    47761 gcactggacc cggtaacgcg cggcgcgttg caacaagaga tgacgcgcat tcaccgtttg
    47821 ctggggcgca ctattgtgct ggtcactcat gatattgatg aggcgctacg gctggcagaa
    47881 catctggtat tgatggatca cggtgaagtg gtgcagcagg ggaatccgct gacgatgctg
    47941 actcgtccgg cgaatgattt tgtccgccag ttttttggac gtagtgaact gggggtgcac
    48001 ctgctttcgt tacgtagcgt ggcggattac gtgcgtcgcg aagaacgggc agaaggtgag
    48061 gcactggcag aagagatgac gctacgcgat gcgctctccc tgtttgtcgc gcggggatgc
    48121 gaggtgctgc cggtggtgaa cacgcagggc cagccttgcg gcacgctgca ttttcaggat
    48181 ctgctggtgg aggcgtaagc gtatgaagat gttgcgcgat ccgctgttct ggctcattgc
    48241 tctttttgtg gcgctgattt tctggctgcc ttacagccag ccgctgtttg ctgccttgtt
    48301 cccacaactg ccacgacccg tttatcagca agaaagtttt gcagctctgg cactggctca
    48361 tttctggctg gtgggaattt cgagtttgtt tgcggtgatc attggcacag gtgccggaat
    48421 tgctgttact cgcccgtggg gcgcggaatt tcgcccactg gtggaaacta ttgccgccgt
    48481 tggacagact tttccgcctg tcgcagtgct ggcgattgcc gttccggtga tcggctttgg
    48541 tctgcaacca gcgattatcg ccttgatcct ttacggtgtg ctgcccatcc tgcaggtgac
    48601 acttgccggg ctgggagcga ttgatcccag cgtgacagaa gttgcgaaag gtatgggaat
    48661 gagtcgaggt cagcgactgc gtaaggtcga gctaccgctg gcggctccgg tgattctggc
    48721 gggcgtgcga acttcggtga ttatcaacat tggtacggcg acgatcgcct caacggtagg
    48781 ggccagtacg ctgggtacgc ccatcatcat cgggcttagc ggatttaata ccgcgtatgt
    48841 gatccagggg gcgttactgg tggcactggc ggcgatcatc gcagaccgcc tgtttgaaag
    48901 gctggtgcag gcgcttagcc agcacgcaaa ataaaggtat aacctgcgag catgacgcca
    48961 ccaattccgc ctaacgccat aaacaggaac agggcgatga ccccaatttt agctatgcgc
    49021 ataatgcact c
//
LOCUS       DAJTUP010000019        47445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTUP010000019 DAJTUP010000000
VERSION     DAJTUP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.019"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..845)
                     /gene="nepI"
                     /locus_tag="OO468_001126"
     CDS             complement(<1..845)
                     /gene="nepI"
                     /locus_tag="OO468_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR6258320.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKL"
     gene            complement(1056..1508)
                     /locus_tag="OO468_001127"
     CDS             complement(1056..1508)
                     /locus_tag="OO468_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR6258321.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(1561..2895)
                     /gene="adeQ"
                     /locus_tag="OO468_001128"
                     /gene_synonym="yicO"
     CDS             complement(1561..2895)
                     /gene="adeQ"
                     /locus_tag="OO468_001128"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR6258322.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3070..4836
                     /gene="adeD"
                     /locus_tag="OO468_001129"
     CDS             3070..4836
                     /gene="adeD"
                     /locus_tag="OO468_001129"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR6258323.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(4882..6273)
                     /gene="uhpT"
                     /locus_tag="OO468_001130"
     CDS             complement(4882..6273)
                     /gene="uhpT"
                     /locus_tag="OO468_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR6258324.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(6411..7730)
                     /gene="uhpC"
                     /locus_tag="OO468_001131"
     CDS             complement(6411..7730)
                     /gene="uhpC"
                     /locus_tag="OO468_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR6258325.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(7740..9242)
                     /gene="uhpB"
                     /locus_tag="OO468_001132"
     CDS             complement(7740..9242)
                     /gene="uhpB"
                     /locus_tag="OO468_001132"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR6258326.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(9242..9832)
                     /gene="uhpA"
                     /locus_tag="OO468_001133"
     CDS             complement(9242..9832)
                     /gene="uhpA"
                     /locus_tag="OO468_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR6258327.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(9907..10197)
                     /gene="ilvN"
                     /locus_tag="OO468_001134"
     CDS             complement(9907..10197)
                     /gene="ilvN"
                     /locus_tag="OO468_001134"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR6258328.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(10201..11889)
                     /gene="ilvB"
                     /locus_tag="OO468_001135"
     CDS             complement(10201..11889)
                     /gene="ilvB"
                     /locus_tag="OO468_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR6258329.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(11995..12093)
                     /gene="ivbL"
                     /locus_tag="OO468_001136"
     CDS             complement(11995..12093)
                     /gene="ivbL"
                     /locus_tag="OO468_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR6258330.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            12658..12747
                     /gene="tisB"
                     /locus_tag="OO468_001137"
     CDS             12658..12747
                     /gene="tisB"
                     /locus_tag="OO468_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR6258331.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13027..14211
                     /gene="emrD"
                     /locus_tag="OO468_001138"
     CDS             13027..14211
                     /gene="emrD"
                     /locus_tag="OO468_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR6258332.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(14219..14716)
                     /locus_tag="OO468_001139"
     CDS             complement(14219..14716)
                     /locus_tag="OO468_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR6258333.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(14713..15075)
                     /locus_tag="OO468_001140"
     CDS             complement(14713..15075)
                     /locus_tag="OO468_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR6258334.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15065..15412)
                     /locus_tag="OO468_001141"
     CDS             complement(15065..15412)
                     /locus_tag="OO468_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR6258335.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            15521..15934
                     /locus_tag="OO468_001142"
     CDS             15521..15934
                     /locus_tag="OO468_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258336.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(15981..17423)
                     /locus_tag="OO468_001143"
                     /pseudo
     CDS             complement(15981..17423)
                     /locus_tag="OO468_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(17458..19173)
                     /locus_tag="OO468_001144"
     CDS             complement(17458..19173)
                     /locus_tag="OO468_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR6258337.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            19340..20233
                     /locus_tag="OO468_001145"
     CDS             19340..20233
                     /locus_tag="OO468_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6258338.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(20230..21044)
                     /locus_tag="OO468_001146"
                     /pseudo
     CDS             complement(20230..21044)
                     /locus_tag="OO468_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21044..22660)
                     /locus_tag="OO468_001147"
     CDS             complement(21044..22660)
                     /locus_tag="OO468_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR6258339.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            22955..23629
                     /locus_tag="OO468_001148"
     CDS             22955..23629
                     /locus_tag="OO468_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6258340.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(23668..25329)
                     /locus_tag="OO468_001149"
     CDS             complement(23668..25329)
                     /locus_tag="OO468_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR6258341.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(25525..25953)
                     /gene="ibpB"
                     /locus_tag="OO468_001150"
     CDS             complement(25525..25953)
                     /gene="ibpB"
                     /locus_tag="OO468_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR6258342.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26065..26478)
                     /gene="ibpA"
                     /locus_tag="OO468_001151"
     CDS             complement(26065..26478)
                     /gene="ibpA"
                     /locus_tag="OO468_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR6258343.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(26472..26544)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            26783..27115
                     /locus_tag="OO468_001152"
     CDS             26783..27115
                     /locus_tag="OO468_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR6258344.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27117..28349)
                     /locus_tag="OO468_001153"
     CDS             complement(27117..28349)
                     /locus_tag="OO468_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR6258345.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            28450..29550
                     /gene="cbrA"
                     /locus_tag="OO468_001154"
     CDS             28450..29550
                     /gene="cbrA"
                     /locus_tag="OO468_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR6258346.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(29511..30848)
                     /locus_tag="OO468_001155"
     CDS             complement(29511..30848)
                     /locus_tag="OO468_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6258347.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(30923..32071)
                     /gene="dgoD"
                     /locus_tag="OO468_001156"
                     /pseudo
     CDS             complement(30923..32071)
                     /gene="dgoD"
                     /locus_tag="OO468_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32068..32685)
                     /gene="dgoA"
                     /locus_tag="OO468_001157"
     CDS             complement(32068..32685)
                     /gene="dgoA"
                     /locus_tag="OO468_001157"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR6258348.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(32669..33547)
                     /gene="dgoK"
                     /locus_tag="OO468_001158"
     CDS             complement(32669..33547)
                     /gene="dgoK"
                     /locus_tag="OO468_001158"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR6258349.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(33544..34233)
                     /gene="dgoR"
                     /locus_tag="OO468_001159"
     CDS             complement(33544..34233)
                     /gene="dgoR"
                     /locus_tag="OO468_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR6258350.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            34511..35167
                     /locus_tag="OO468_001160"
     CDS             34511..35167
                     /locus_tag="OO468_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258351.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(35213..36025)
                     /gene="yidA"
                     /locus_tag="OO468_001161"
     CDS             complement(35213..36025)
                     /gene="yidA"
                     /locus_tag="OO468_001161"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR6258352.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(36139..36537)
                     /locus_tag="OO468_001162"
     CDS             complement(36139..36537)
                     /locus_tag="OO468_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR6258353.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(36777..39191)
                     /gene="gyrB"
                     /locus_tag="OO468_001163"
     CDS             complement(36777..39191)
                     /gene="gyrB"
                     /locus_tag="OO468_001163"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR6258354.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(39220..40293)
                     /gene="recF"
                     /locus_tag="OO468_001164"
     CDS             complement(39220..40293)
                     /gene="recF"
                     /locus_tag="OO468_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR6258355.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(40293..41393)
                     /gene="dnaN"
                     /locus_tag="OO468_001165"
     CDS             complement(40293..41393)
                     /gene="dnaN"
                     /locus_tag="OO468_001165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR6258356.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(41398..42801)
                     /gene="dnaA"
                     /locus_tag="OO468_001166"
     CDS             complement(41398..42801)
                     /gene="dnaA"
                     /locus_tag="OO468_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR6258357.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43098..43178
                     /locus_tag="OO468_001167"
     CDS             43098..43178
                     /locus_tag="OO468_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258358.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            43408..43548
                     /gene="rpmH"
                     /locus_tag="OO468_001168"
     CDS             43408..43548
                     /gene="rpmH"
                     /locus_tag="OO468_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR6258359.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            43565..43924
                     /gene="rnpA"
                     /locus_tag="OO468_001169"
     CDS             43565..43924
                     /gene="rnpA"
                     /locus_tag="OO468_001169"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR6258360.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            43888..44145
                     /gene="yidD"
                     /locus_tag="OO468_001170"
     CDS             43888..44145
                     /gene="yidD"
                     /locus_tag="OO468_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR6258361.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            44148..45794
                     /gene="yidC"
                     /locus_tag="OO468_001171"
     CDS             44148..45794
                     /gene="yidC"
                     /locus_tag="OO468_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR6258362.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKSMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            45900..47264
                     /gene="mnmE"
                     /locus_tag="OO468_001172"
     CDS             45900..47264
                     /gene="mnmE"
                     /locus_tag="OO468_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR6258363.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11701 a        12850 c        12033 g        10861 t
ORIGIN      
        1 agttttaccg aacgtttaag aatgaacgac gaaagcgacg taccaataaa gctggcgata
       61 ccaaaactca acagcaccag cgttaagcca tccacgccga atcccgccag gttcatatac
      121 accgggcgaa tatacgtgaa gaaagcaaac tgcccggcga aagacatgaa gatggcgatc
      181 atccctgcca tcacacccgg acgttgtaat aagcggaaag tattttgttt ctgatgcgag
      241 ggttcgcctg gcagtgaagg caatgatttg ataatccaga aaatacacag cacgcccatc
      301 accgccgccg cattaaagac attgcgccaa ccgataagct cgcctaaaaa actgcccaac
      361 ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga tcaccgacag cgccttcggc
      421 accgtacgcg gcggtaccag acgcatggtc agcgacgccg acatcgccca gaacccgccc
      481 agcgccagcc ccagacaggc acgaccgatt aaaagcaaac tgaatgagtt agcaaaggaa
      541 accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa taacaacgta gcggcggtca
      601 gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca ttgccacaaa ggcggtcacg
      661 gtcaccgatt gcccggcaac cccttccgaa atgcctaaat cctgggccat tggcgtcaac
      721 aaactgacgg gcaaaaactc aacgataatc agacaggcga cacaaaacgc caccgagaaa
      781 acggctgacc agttcggtcg ggtgatggca tccgcgccgc ggttttcggc aataaattca
      841 ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg accactttaa cattgaaatg
      901 tgtgacgaat ttaacgtttt tgcaactttt acggtgttgc ttgagctgga gttaagatcg
      961 aaacggaaca gggcaaagat taccggatgc ggcgtgaacg tcttatccac ccgacaggtc
     1021 aggcgcggtc tgtaggctga taagacgaaa cggtttcaat gcaaccagtg aacgccctcg
     1081 gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat taatatcctg catttcaggt
     1141 tcggtttcac gcagaaaacc cagcgccagc ctgacctgag catcggtaat cggcgcggaa
     1201 aggcgggctt tttgtaaaat ccggtgccat tgcagcaggt tctgttcgct atcatcgaca
     1261 atgacaattt gccagataag caacacctgc gcggcgtttt gcaggtgcga ctcatccggg
     1321 cgatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc ccatttgatc gaccatcgac
     1381 tccacgggta cgacatcgat attcagccga tcgctgagac ggcgaatcgc ttttctggcc
     1441 ccggatgtca gcacccgaaa accaaccaca aacactaccg ccagcgtggc gagcattatc
     1501 cagatcatga gttctcctgc gtaatcagaa ctcatgatat ctggaaaccc tcgccagtta
     1561 ctaatccacc agaataatct tcaatgcgaa cagagctgcg accaccacca cacacaggtt
     1621 cagatcgcgc cagcgcccgg tgcacacttt catgatgcag tacgacataa agccgagtgc
     1681 aatcccttcg gtgatcgaga aagtaaaggg catcatcacc gtggtaataa acgcaggcac
     1741 cgattcggta aaatcatccc agttaacgcg cgccaggctc gaagtcatca gcacgccaac
     1801 aaagattaac gctccagcgg ttgcgtaagg aggaaccatc gccaccagcg gtgagaagaa
     1861 cataactaac aggaacataa cgccgaccac gacagccgtt agccccgtgc gaccaccgac
     1921 tgccacacca gaagtacttt caatataggc agtaacagac gaggtaccga taaatgcgcc
     1981 tgccaccgaa ctgacgctat caacatacag cgccttattc atattgggga atttaccgtt
     2041 gctatctatt aagcccgctt tatcagttac accaattaat gttcctgatg aatcaaatag
     2101 gttgatcagc ataaaggaga aaatgatacc ggcgagttca agtgttaacg cgccgctcaa
     2161 atctacttca ccaatgacgc cgctaatatc aggcggaatg gaatagacgc cgctaaaatg
     2221 aacatcaccg aaaaataatc cacagcaaga cgtcaccaca atagaaacca gcaccgcggc
     2281 atgaaaatga cgtgatgaca acacggttat aataaaaaac cctaaaatac ctaacaacac
     2341 gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct ttattggcga caataacacc
     2401 agtgtttttt aatcccatta aggcaataaa taatccgatt ccactggtga taccaatacg
     2461 taaacttaat ggaatgttgg agatcatcca gtaccggata cgaaacagcg tgagcaaaaa
     2521 tagcccaatt gcgccccaga atatcgcgcc catcccggtc tgccaggaga tccccatcgc
     2581 ccccacgacc acgaacgcaa agaaggcgtt cagccccatt gccggagcca gcgccacggg
     2641 taagttagca aatatcccca tcgcaatact gccgataccg gcaatcaaac aggtggtaac
     2701 aaacaccact ttcgggtcca tttgtgccgc gccgaggatt tgcgggttca caaaaacaat
     2761 gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat tctgtgcgga cggtggtccc
     2821 atgctgaggt agtttaaata atcgcgaaag cgtccctgat tcattactca cgtaatcggt
     2881 attgtcatta ttcatttttt tgtccattgg aggagattta atcccttgct tttgtaaaac
     2941 aaatgacaac aacggttcag tgataattat cacatttcaa ttgcacatta atagatattc
     3001 tttaataatc tcgcgacgtt tctttatgat aaataataat caaattgata aaatcaaaat
     3061 gagaaaaata tgaataattc cattaaccat aaatttcatc acattagccg ggctgaatac
     3121 caggaattgt tagccgtttc ccgtggcgac gctgttgccg attatattat tgataatgtc
     3181 tctattctcg acctgatcaa tgccggagaa atttccggcc caattgtgat taaaggacgt
     3241 tacattgccg gtgttggcgc agaatacgct gatactccgg ctttgcagcg gattgatgct
     3301 cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc tgcatattga atccagcatg
     3361 atgacgccgg tcacttttga aaccgctacc ctgccgcgcg gcctgacgac cgttatttgc
     3421 gaccctcatg aaatcgtcaa cgtgatggga gaagccggat tcgcctggtt tgcccgctgt
     3481 gccgaacagg caaggcaaaa ccagtactta caggtcagct cttgcgtgcc cgctctggaa
     3541 ggctgcgatg ttaacggtgc cagttttacc cttgaacaga tgctcgcctg gcgggaccat
     3601 ccgcaggtta ccggccttgc agaaatgatg gactaccctg gcgtaattaa cgggcagaat
     3661 gcgctgctcg ataaactcga tgcatttcgc tacctgacgc tggacggtca ctgtccgggt
     3721 ttgggtggca aagaacttaa cgcctatatt gctgcgggta ttgaaaactg ccacgaaagt
     3781 tatcagctgg aagaaggacg ccggaaatta caactcggca tgtcgttgat gatccgcgaa
     3841 gggtccgctg ccagcaatct caacgcactg gcaccgttga tcaacgaatt taacagcccg
     3901 caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga tcgcccatga agggcatatc
     3961 gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc cgctgcatgt ggcatatcgc
     4021 gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc acctcggctt actggcaccc
     4081 ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta aggtcacggt gcagcaggta
     4141 ctggtgaaag gcgaaccgat tgatgcgcaa accttacagg cggaagagtc ggcgagactg
     4201 gcgctgtctg ccccgccata tggcaatacc attgcccgcc agccagtttc cgccagcgac
     4261 tttgccctgc aattcacgcc cggaaaacgc tatcgggtca ttgacgtcat ccataacgaa
     4321 ttgattacgc actcccgctc cagcgtctac agcgaaaatg gttttgatcg cgatgatgtg
     4381 tgctttattg ccgtacttga gcgttacggg caacggctgg ctccggcctg tggtttgctc
     4441 ggcggctttg gcctgaatga aggcgcgctg gctgcgacag tcagccatga cagccataat
     4501 attgtggtga tcggtcgcag tgccgaagag atggcgctgg cggtcaatca ggtgattcag
     4561 gatggcggcg ggctgtgcgt ggtacgtaac ggccaagtac aaagtcatct gccgttaccc
     4621 attgccgggc tgatgagcac cgacacggcg cagtcgctgg cggaacaaat tgacgccttg
     4681 aaagccgccg cccgtgaatg cggtccgtta cccgatgagc cgtttattca gatggcgttt
     4741 ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc aggggctatt tgatggcgag
     4801 aagtttgcct tcactacgct ggaagtcacg gaataataaa aaaagcccgg cgtcatgccg
     4861 ggcaaaagtc accagttacg tttatgccac tgtcaactgc tgaatttttt tctcgcggcg
     4921 gattttgcgt tcttccatta ccgccactat cgccatcagg cagatacaac caatcgcggc
     4981 gatatccagc gcggcgaagg tgcctgccca gccggtaagg ccgaataccg gcgtcccatc
     5041 ggcaatcatt cccagaccta acttggcaaa gctgtcacca atcaggtaag caaaggtgcc
     5101 tttaatacca tcggcagcgc caatcgcttt tttaggtaca aagccaacag cagccacacc
     5161 aatcaacaat tgcgggccaa agaccaggaa acccaacgca aagagagaag ccagatagat
     5221 atattcgtta ctggcatgtt gatacacacc gagcgtggcg ataatcagcg ccagcgcgat
     5281 gcaggccacc aggccacggc gaccgttcgc caggtcagag agccagcccc acagcagcgt
     5341 accgaccagc gcaccagctt caaacagcgt aaagccctga atcgccaccg ctttagagag
     5401 tttcagttcc tggaacgcgt atacggttga ccactggtcg ataccaatac gtaccacata
     5461 gaggaaaatg ttggcgaagc acagcagcca gatcactttg tttttcagca catactcaac
     5521 aaagatctgc cacttggtca tatcggtaga ttctgtctct ttgtcctctt cgctgatctc
     5581 ctcgccgaac agttcttcag ctttgccgag gccataagat tccggggagt cgctgccgta
     5641 acgcaggccg ataaaaccga caatcagcgc gataatcgac gggaagataa acatgccgat
     5701 gacatggcca tcgaacaggt aatttgcccc gaacagcgcc acacctgctg cgcctgcacc
     5761 gccaaggttg tgagaaatat tccagaaacc gaggaatgtc ccgcgtttac gacgcggcgt
     5821 ccatttggtg atggtggagt aactgcacga accgccggta ctctggaaaa agccgcttaa
     5881 ggcgtagaag gcaatcatca ggaacaggct aaccgagccg ctgcccatac tggcactgaa
     5941 gcccagcata caaatggcag agaggataag catgaacggc aggaattgtt tggtgttttt
     6001 gccgtcggcg tagtatgaaa ccaacgtttt acccacgcca taagtgatgg agaaacccag
     6061 gccgatcatc cccagctgcg tcatgctcaa cccgtaggtc gaaatcatat cgttctgcgc
     6121 gatgttaaag ttcttgcgaa tcaggtacat cgtcaggtag ccgataaaga ccaccaggta
     6181 ggattgcatg aacggtttga accacatttt gcgccgcact tcgagcggaa ggtccagggt
     6241 cggcttgcga acttggttta ggaaagccag catgggttac tcctgaaatg aatacctgcg
     6301 tgaggcatgc attgtaaaaa tcagcagcaa atcaggcctg agatagcgtc caagaaaaac
     6361 ctggaaaatt tcttagtttt gccggatatg aagtgaaaag gtgagatgca tcacgcttca
     6421 cgcggtgtct gggcgttcaa aaagggcagt aacagcagtg cggaaatccc ggcggcgata
     6481 gagataacca caaaaaatcc gctccagtgc caggtatcga gcactttcgc cagcggccaa
     6541 ccagcaagcg acgcccccag ataagcaaac aagccgacaa accccgtcgc cgcccctgcc
     6601 gcctctttgt gggaacactc tgccgccgcc ataccaatta acatctgcgg gccaaagaca
     6661 aaaaaaccaa tggtgaagaa gcaggttgcc tgcatcacgt agctggcaaa tggcatcagc
     6721 cacagcgagc cgactgaaag caaaattccg gcggcgaaaa tcaaattcat cggcccacgg
     6781 ttgccgttaa acaatttgtc cgagccccaa ccggctacca gcgcaccgat aaatccgccc
     6841 agttcaaaca tcgtcactgc cgtattcgcc gtgaccagat cgacgcccag cgtctcggac
     6901 atatacaaat tgccccagtc gttgatcgcc gcccggacca catagaccag cacatagcaa
     6961 aacgaaagca gccagatata cggattcagc aacacatatt tggtgaggat ctctttacgc
     7021 gtcaaccctg ccccttcttg ttgttgagca atttccagcg cgtcgtgccg ccattcaccg
     7081 accgccggta aacctaacgc ctgcgggcga tcgcgtagcc gccagcagag aaaaatcccc
     7141 acgactatcg ccatacaacc agcaatcatc atcccggcac gccagccgta atgcagcgcg
     7201 ctcgctgcca tcacaatggg aatgagtgcg ccgccgacgt tatgcgccgt gttccataat
     7261 gcccaccaac cgccgcgctc ggtacgtgaa taccaggccg ttaacagacg cgcacacacc
     7321 ggtgaacccc agccctggaa aaaggcgttc agcacccaga gcacggcaaa cgcccatagc
     7381 gacgtcgaga agccaaacag aatgttcatg atgcccgtgg cgataagccc tatccccata
     7441 aaataacggg catttgagcg atcgctgaca atgccggaga caaacttcga cacgccgtaa
     7501 gtaatgtaaa acagggtcgc taacaggccg atatcgctac ggctgagcac gccgttagca
     7561 aggatttctg gtaccgcggc gttaaaactt ttccgcgtga agtaaaacag cgcgtaaccc
     7621 agccagatgg tcagcagaat atgccgacgc cagtagcgat aacgggcatc aatttcatgt
     7681 ttatcagtca ttaatggcgc atcggcaggc gctttcagaa acggcaacat catcgaacct
     7741 tagacataac gttgaggtag agaaacgctg acacgcgtgc cgtgcagaca ggaaatgtgt
     7801 aatgtgccgc ccagcgccgt tacgcgctcg cgcattccgg tgaggccaaa accttgttgc
     7861 ccggaacccg gcggcaaacc gctgccatcg tcttcaatca ccagcatcaa ccgttcatcc
     7921 tgctgccagc cttgcagcgt aaccgcgctg gcatcggcat gtttcacaat gttgttcagc
     7981 ccttcctggc agacacgaaa cagcgtcacg cgctggtttt cgcttaatgc tgattcatcg
     8041 atacgccatt cgagatggct gacaatgccg cgcccttcca gctccatttc ccgcatcagt
     8101 gagcggatgg cctgctccag ggtgagatca tccagctggc gcggacgtaa ccgtcctaac
     8161 aaacggcgca ccgcgtcgta aacgcccagc gacagttgtt cgatgagctg cccgctctgc
     8221 ttcacactgg cgttatctgc cgccagccgc tgaacaatgc ccgcctgagt acgaatagca
     8281 gtgatggtct gaccgatatc gtcgtgcagc tcacgcgcca cgtcgcggcg cacgctctct
     8341 tcggtttcta acaaacgttc agccagatgc tgattgcgcg ccagttcctt ttgtagcgac
     8401 tggttaagtt cacgcaaccg ctggatgcct gcgcccagta acaaccctgt cagactttgc
     8461 accagcaacg agagtaataa atccaccaga tgatcgcgcc aggtttgact ggcgatcagc
     8521 gcgatggcgt tcatcaacgt cgcaatcagc gctccttgcc agccatagtg ccaggccagc
     8581 gcgataatcg gcagcgccag acagaatggc gtaaagcgcg acagttcgtc cggcaatccc
     8641 aactggagcc agagactgat aacaaacagc agcaagtacc agaccagatg ccgcccgcgc
     8701 cagttgatgg gctgagaaac cagtgacggg ccgagcggca gccaggtgtt attggcgaga
     8761 tagtgccaga acaccagaca tatcggggcc agcgtcagac cgccagttaa agtcagcaac
     8821 agcgcattcc acgactcttt gccgtgccaa agccagggca gcgactgcaa caacgccgcc
     8881 gccgttaacg ccgcaccctg taacagcaag gtgcgccagt cacgctgatg gcgatagcgc
     8941 gagatcagcg ctacgggcag taacgtcagc aaactaccga tcatcaataa cggaaaatgg
     9001 gttaaaccga ccgcctgcgt tagccagtaa atcagcagcc actccgcacc cagcaatacg
     9061 ggccagtatc cgcgcgggca ttgcagcatt agccccagac gcagaccaaa cggaaataac
     9121 agcaccgcca tatcagggcg ctcaaccaga tgcaggctga tactccatag gcaaaaccat
     9181 gcggcagaga agataaaaaa gcaggcaata acggtaatta agcgggagaa caacgtcttc
     9241 atcaccagcc atcaaacatg cggcgcgcca gctctacgtc gttactgacg cccagttttt
     9301 ccatcagatt ggcgcgatgg acgtgtaccg ttttcggtga caagcccagt tcggcggcaa
     9361 tctctttcac cgccattcct tgcgccagtt tttccgccac ctggcgttca cgtttggtca
     9421 gcgggtcctg acgaccggat gccagtttaa tggcaatatc cggcgtcaga taacagccgc
     9481 ccgtggcaac cgtatgcacc gcagcaatga gttcatccgg gctacagcgt ttggaaagaa
     9541 agccgcgtgc ccccgcgtta agcgcctgct caaccagcgc aggactgtcg tgaacggaga
     9601 gcataatcgt cgccatacct ttcggcagct ggcttagcag ctccagaccg gagatatcgg
     9661 gcatggagat atcgcaaata cacacctgca caccgcgccc cggcagcccc gccagcgcct
     9721 cgcgccccga accaaactcg gcaactacct gcaaatcagg ttccagcccc agcagctgcg
     9781 caaagccgga gcggacgatg aggtgatcgt ctataagggc aacggtgatc atggtcttgt
     9841 cctggcgggt aaaaaaaaac gcgcttacct taacgataag cgcgatgttg ttcaagcctt
     9901 gagcggttac tgaaaaaaca ccgcgatctt gttaaacatc gtcggatcgg actgattacg
     9961 ctgcactttc acgacatctt ccagcttagc gatttggctt atcatctgct ccagacgctg
    10021 gtcgtcattg accagtagcc agatatggct tttgtcgctg tcctgaatcg gcagacaaag
    10081 aatgccttca acgttaaaag cgcggcgggc aaaaaggcca caaacgtggg tcattacgcc
    10141 cggatggttg cgaacggtga gctccagaat tacgttgtta tgagttgtgt tttgcatggc
    10201 ttattccccc accatttcag tattcgccgc acctggcggc accatcgggt aaactttttc
    10261 ttcggcatca atgcgcacat ggatcagcgc cgggccaggg cgattgatga tttcctgcaa
    10321 tgcagcctgc ggatcggctt cgttattcaa atcacaggtt tcgaggccga atccggcggc
    10381 aatctgcata aagttgattt tgcccggata ggtggcggca aaaacgcctt gctcgtagaa
    10441 cagactctgt tgctgatgca ccagccccag cgcttcgttg ttcatcagaa tgattttgac
    10501 atccagctga ttttcactgg cggtcgccat ctcctgaata ttcatcatca ggctgccgtc
    10561 gccggagaaa cacaacactt tgcgatccgg gttcgccagc gccgcgccaa tcgccgcagg
    10621 caggccaaaa cccatcgtgc ccagcccacc ggaggtcagc cactggcgtg ggcgattgag
    10681 cggataagct tgcgcggtcc acatctgatg ctgacccaca tcggtggtga taatcgcatt
    10741 gtcatcgaca caggcggcaa cggcgttgat caggccgtaa tggcttaatg gatcgcacgc
    10801 tttcgggatt ggacacggga attcgcgctg caaatccgct accaactggt gccactctgc
    10861 acgcggttgc gcttccaccc gcgggatcaa ttgcgccagc acgtcatcaa catccgcctg
    10921 aatcgccaca tgcggctgct tgattttacc cagctctgcg cgatcgatat cgacatgaat
    10981 aattttggca ttcggacaga actgctcggt tttgccaatc gcccggtcat caaaacgcgc
    11041 accgagcaca atcaacaaat ccgcctcctg caaaatatag ttggtactgc gcacgccgtg
    11101 catccccagc atacccagcg acaacggatg cgcttttggc agcatgccca gcgccattaa
    11161 agtcatggtg gtaggcagtt gcgctttctc cgccagttca cgcacccgcg caggcgcatt
    11221 gatcacacca ccacccagat aaagcaccgg gcgtttggca gcgttaatca ttgtagctgc
    11281 gtcacgaatg ctttcttcgc taaaggcggg tgcaacggct ttttctgcca ccgcgggctg
    11341 agcttcaatc tcaaaaaccg ccgtttgtac atccttagga atgtctatcc acaccgggct
    11401 agggcggcct gattgcgcaa tgcggaaggc atcgctcatg acctgtggga gctcttcgat
    11461 atgtctgacc agatagttgt gtttggtgat ggggatagag atgccgtagg tgtccacttc
    11521 ttggaaggcg tcggtgccga tcatcgaggc gggaacctga ccagtgatgc aaatcagcgg
    11581 gatggagtcc agccgcgcat cagcaatggc ggtcaccagg ttagtcgcac ccggcccgct
    11641 acaggccata cagaccgccg gtttaccgtc ggtgcgcgcc attccctgag cgataaaccc
    11701 cgcgccctgt tcatggcgag ccagaatatg gcggatttgc gtgctttggc ttaaggcgtc
    11761 gtaaacaggc aggatagaac cgcccggaat gcccgtcaca atcttaatgc cctgctgttc
    11821 caggaaatga acgataaatt ctgcgccggt aaagcgctta cgcgtcgatg ttgtgcccga
    11881 acttgccatg ctccagtcct tttcttctgg gccgactttc cgggaggtgc ttaaacgaaa
    11941 aaccccgccc ggtttgcgcc ggcggggttt tggaatcgtg tgttgttcca gtccctacgg
    12001 cgcattgccg acgaccacca ccacacgcac gacgaccact gcggcggatg gcgcagttgg
    12061 tagtagtttt gcgttgaaca tggaagtagt cattggggac cttgttggtt ttgtgtttaa
    12121 caatatttat acaagcacag ctttacaggg gagacaatgg aaaatttttc agcaagggaa
    12181 aattgagggg ttgatcacgt tttgtactga attgcagata acaaaaaacc ccgccggagc
    12241 gaggtttcgt cagccgcctg cggctggtaa ccgcaaagca cactgtatta tgtcaacact
    12301 gaaagtatac gtgttccgcg cagaacgcgc aatttcggca cgaattttga cgtatttagt
    12361 gcatagttga gtatcgttca cagtttgcgt tttgtccaaa tattactgtt tatttataca
    12421 gtaagcttct ataatatcac tgtacgcaat gtgttatgcg ggggccgcat cgttacccgg
    12481 cgcactaagt cctggctgaa acgggtggtg ccgtcagcgc cttaaccccg cgtgagcaca
    12541 ctgtgttatg tcaacaagca caacgtttct ccttgagata ccgcgtgcac aacagctggc
    12601 aacaggcagc ggaaaggtac gtcagctggc agtgctcctg aaccacagga gacgcgtatg
    12661 agcctggtgg atatcgccat tcttatcctc aaactcattg ttgcagcact gcaactgctt
    12721 gatgctgttc tgaaatacct gaagtaattc agattcaagt cgcaccaaag gggagcgggt
    12781 aaccgctccc cttttatatt tagcgtgcgg gttggagtcg gatgcgatgc tgacgcatct
    12841 tatccgccct accatccctc ccggcaacat ttattgccgc ttttgtttac atattctgcc
    12901 gctaaacaat tccccattcc tggcgtatat ctggctaaca ttcatcaatg tgatagattc
    12961 ctctcccgca tttatgggaa tgcgtagtga cttattctaa ttatttttat aaaagcatcc
    13021 gtgataatga aaaggcaaag aaacgtcaat ttgttattga tgttggtatt actcgtggcc
    13081 gtcggtcaga tggcgcaaac catttatatt ccagctattg ccgatatggc gcgcgatctc
    13141 aacgtccgtg aaggggcggt gcagagcgta atgggcgctt atctgctgac ttacggtgtc
    13201 tcacagctgt tttatggccc gatatccgac cgtgtcggtc gccgaccagt gatcctcgtc
    13261 ggaatgtcca tttttatgct ggcaacgctg gtcgcggtca cgacctccag tttgacggta
    13321 ttgattgccg ccagcgcgat gcaggggatg ggcaccggcg ttggcggcgt aatggcgcgt
    13381 actttgccgc gtgatttata tgaacggaca cagttgcgcc atgctaacag cctgttaaac
    13441 atggggattc tcgtcagtcc gttgctcgca ccgctaatcg gcggtctgct ggatacgatg
    13501 tggaactggc gcgcctgtta tctctttttg ttggttcttt gtgctggtgt gaccttcagt
    13561 atggcccgct ggatgccgga aacgcgtccg gtcgatgcac cgcgcacgcg cctgcttacc
    13621 agttataaaa cgcttttcgg taacagcggt tttaactgtt atttgctgat gctgattggc
    13681 ggtctggccg ggattgctgc ctttgaagcc tgctccggcg tgctgatggg cgcggtgtta
    13741 gggctgagca gtatgacggt cagtattttg tttattctgc cgattccggc ggcatttttt
    13801 ggggcatggt ttgccgggcg tcctaataaa cgcttctcaa cgttgatgtg gcagtcggtt
    13861 atctgctgcc tgctggctgg cttgctgatg tggatccccg actggtttgg cgtgatgaat
    13921 gtctggacgc tgctcgttcc cgccgcgctg ttctttttcg gtgccgggat gctgtttccg
    13981 ctggcgacca gcggcgcgat ggagccgttc cccttcctgg cgggcacggc tggtgcgctg
    14041 gtcggcggtc tgcaaaacat tggttccggc gtgctggcgt cgctctctgc gatgttgccg
    14101 caaaccggtc agggcagcct ggggttgttg atgaccttaa tgggattgtt gatcgtgctg
    14161 tgctggctgc cgctggcgac gcggatgtcg catcaggggc agcccgttta agcgcacgtc
    14221 accgcagcat cgtcatcagc tccatgggag aacgatgctg ctttatcaga tcacgcatca
    14281 cccgcatatg cggtgcggag taagaataaa acgcctgata gcccgcacaa agcacgctgt
    14341 ctggcgtctc ctgccgagcg tgcgggcaca ggcgatgcca gtcgcacgcc tggcagtcac
    14401 ggcgcggttg cgggttttcg ctgccacacc atttttgtaa ggtggcgtca aaaagctcaa
    14461 tcgagatacg actgatatct gcccgcaccc aacactcaaa cagggcaatt aaaaatttcc
    14521 cccacagctc tgctgtcacc gaagcggccg tcagatcgcc ccggtcatca aattcaagat
    14581 agggaatgag ctgtacggag cggatgcccc gctgtttgaa attgtgataa aaatcggcag
    14641 gttgcagagc atccttgtac tctacaacca gcttatgcct gtcctcttca gcatcaatga
    14701 cctgacttcc tgtcatgcga ctctctcaat aaatacgata agttgatgta tatgcgttac
    14761 agcaaacagt attgcgagag ataacaccgc gagggagatc aaaaatttgt cacggactac
    14821 gtgaaattgg gaaaaatcgc tattcgtgac atccattaaa ttgcgattac gggtgtagtg
    14881 ccagaggatc agcgccacga tggcgaggat gccaatcgaa atccagaata acatgcccgc
    14941 ctggtgccag ttgtgtttga tagccaacgc catcaacgcg ccgtagccca gcatggtacg
    15001 aaaccacgcc agtgatgtgc gctccggctg cagccctggg tcggcaatgc gtctggcttt
    15061 acggctatcc ggcatacaac accagtccca taacgatcac cgccacaacc atcaatatta
    15121 agctgatgat taataagctg ttggtgtagg gcaggtcctc tttcagacgc atcgcttttt
    15181 cattgcgtaa ccagcgcagg tagccataca ttgctaaccc gccggaaaac agacacaaca
    15241 acaacgccag cagttcacga atgacgggcg tggcgaaatc tggcgcaagt tgatcaagcc
    15301 caacccccgc cgccagaaat cctagtgcgg tacggatcca cgccagaaaa gtacgctcat
    15361 tagccagcga gaagcggtaa tcgggcgctt ctccaaggcg ggaaattttc attgttgctc
    15421 ctttgcaatt tcactaacgc tctatcttta gcggtttttt ttaagaattt cgtccgcccg
    15481 tttcctgata tgcttttgcc atccgctaca aggaggtcag atgggtatca tagcgcaaaa
    15541 taaaatcagt tcattgggca tgctgttcgg tgccatcgct ttaatgatgg ggattatcca
    15601 cttttcattc ggcccttttt ccgcaccacc gccaacgctt gaaagcatcg tggcggacaa
    15661 aacggctgaa attaaaagag gtcttctcgc cggaataaaa ggcgaaaaaa ttaccactgt
    15721 ggagaaaaaa gaggacatgg atatcgataa aatcctcgat caaagcggta tcgcactggc
    15781 cattgctgcg ctactttgcg catttattgg cggtatgcgt aaggaaaatc gctggggcat
    15841 tcgcggcgcg ctggtttttg gtatcggcat cgtgtgcagc attttgctga tattcctgat
    15901 tttttccttc ctgaccggcg gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg
    15961 atcatcagcg ggcattatcg tcagaatttc tgttttttct cctccacctt cactccttgc
    16021 gtcggtaggc cggtgtcata gtcgcgtacc actggcgaat agccatcttg tgggcgtggg
    16081 cgaaacgccc ccatccagcg cggtcgtgca tctttacgcc acggacgcag actccattgg
    16141 taactgcgga acggatcgcg aattttgtcc atgtaatcca ataaggcgtc atgcattttg
    16201 ctgcgaacgt ctgcaaaacg gatatcatcg atcaggttat gcatttcatt tgggtcatta
    16261 cggcgatcgt aaagttcatc actggtgaag aggttgagca ccagtttaaa gtcatccgtc
    16321 acccagcaac gcaccggaat aaaaccgcca aagctgtcat gctcaatctc gtagcggtta
    16381 aattccacca tcacgccgcg tggctctttc acggcaagga tattttcccc cggcagaatc
    16441 tctggttttt caatatctgc cagcgccatc attgtcggca gtaaatcaat atgactgact
    16501 ggcgtatcga cctgtcgccg ctccccttgc ggcgaacgga tgatcagcgg aatacgggtg
    16561 atgtcgtcat acatcgccgc tcctttactg atcagcttat gtgcgcccat catttcaccg
    16621 tgatcggagg tataaatgac ccacgtattt tcacgttgct ctggcgttaa ggcattgatg
    16681 acacgtccga tttggtcatc aacaaagtca ttgcaggcaa aatagagcgg atggtgataa
    16741 agcccgtcat caccgactgg cgatggcatc gcctgcgccc ataagcggtg atgttccggt
    16801 ttgttcgcca ggtcatcctg tgatttctcg ccaagttcgt agtaaaaatc agcgtatttc
    16861 tctaaatact ccaccggaca ggtgaacggg tgatgcggct catcatacga aaccaccatc
    16921 aggaagggtt cctcggcgcg cacgggctat tgcagaaaat ctaccgctcg attgctgatg
    16981 cggtgcgccc aggtgaaggt ttcgtcaata tggttcgcct gtaaatcttc gacgctgttt
    17041 aggccattgc gccacaggct gatctctttt tccgtcagtt cgctaagata gttcgcccca
    17101 tcaaaccagt aatcagcatc ccactccggc ggacactcgc cagtgccgaa atagtcatga
    17161 ccgtcgagat gccatttgcc gatgtaacag gtgtgatagc cggcatcctt aaagtagcgc
    17221 cccatagtgg agatgttttt gcctggcgcg acgttgttgg tccacgggcc ggactggtta
    17281 gcgtagatac cggtgaatag tccggcccgt gcaggcgtac aaaccggtga acaagtgtag
    17341 gcggaattaa agcgaatacc ttccgccgcc agactatcaa tattttgcgt attcagcggt
    17401 ttaccgctat agcaaccgac catattggtg gcctgggtat cggtcaattg gggcgtttca
    17461 taggctatcc ttcgcgtcgg gagtaaaggt tacggctggg tcgtgacgat gccaccagct
    17521 gtcaaaaatc aggtagacaa tcactacggc agcgatgaaa taactgatca tcgctaacca
    17581 gcgcgtaccg tagccgccga attcagccag cccgacatag acgccaatca tcgcgaacag
    17641 gataccaatt gacgcgatct tgacgttttt ccacggtttc atgtccaccg caaacgcatc
    17701 tttgaaggtg aacggcgtgg cgcgcggttt gataaaaccg atcaccagca tcacgaccac
    17761 gttgatgcag aacgtacagg ccagcacata gaggaaatgg aagtcgaact tcaccagata
    17821 gttgatggtg atgtagctga ttatgccaat ccccatcgcc acttttgccg ccagcgccgg
    17881 gatgcgcgga aagaaaaagc ccataatgat gatggtaacc agcggcacgt tgtaaatgcc
    17941 gttgagctgt ttcatccagc tatacagccc ctgcggcgcg ttcgcgatcc acggcgctac
    18001 caaaaccgaa acgatagcga taaagaaacc aaatttgcgc ccgacggtga ccagctgctg
    18061 cggctcggca ttctggttaa tgatgcgacg gtaaataccc atactgaata atgtactggc
    18121 gctattcaga aagccgttga aggtactgat caccgcacca aataacaccg cgccgaagaa
    18181 accctccagt ggcactggca gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt
    18241 cggtagatcc tgatacagat gaaacgcaat caaccctggc agcaccagta ccagcgggtc
    18301 gagcattttc agcaccgccg ttaacagcgc ccccttttgc ccttccgcca ggctttttga
    18361 cgccagcgtg cgttgcacga tgccctgatt tgtacaccag taaaaggtgt tcaccagaat
    18421 taaaccggta aatgccgcgc caatcggcaa gggatcggtc gggccaccga ctgagtttaa
    18481 tttctcggcg tgaacggtgg taatttgctc aatgccctgc ataaagctgc ccttgcctat
    18541 cgcgatcagg ccaaataccg gcaccatcaa cccgccgata accagcccaa taccgttgat
    18601 ggagtccgcc actgccattg cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag
    18661 accaagcaaa attaccagta gccagatagc cgcaccgtga gaaatctgta gcgattcccc
    18721 gacgtgaaac aggctgttga gcgccaacgc gccggagtag agaacaatcg gcagaaagca
    18781 gacgccggtg gcaatgagga agcagaagtc gatgataata cgcgtcgttt tatcataacg
    18841 ttcctccaga aaatcgggga tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat
    18901 tagcgcgagg aagatcagcg tcaccgctga agtcacttcc cagcccatca ccgacatgcc
    18961 gcttttgtag gcctgcccgg aaagaccaac cagttgttcc gtggaaaggt tggttaacat
    19021 taacgaagcg gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat agccttgttg
    19081 cgatccggta tctgttttgc gaaccttcca ccaggtgatc accgccacca gcagcgtaaa
    19141 accgacaaaa ctcaagattt gtaacgaatt catctcatag cccttattta tgtttatgtc
    19201 ccggacgcat gaatataggg agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa
    19261 atacaaacgt cgtatccctg aacggattgt tttctgttaa gttcaggttg tgagcataat
    19321 tcgccggagg gatattaaaa tgaatggaaa attgcaaagt tcagatgtaa aaaacgaaac
    19381 tccgtacaat attcccttat tgattaacga aaatgtgatc tccagcggaa tttctctgat
    19441 ctcgctctgg catacctacg ccgacgagca ttaccgggtg atctggccgc gggacaagaa
    19501 gaaaccgctt atcgccaact catgggttgc ggtgtatacc gtacaaggat gtgggaaaat
    19561 tcttttaaag aatggcgaac aaataactct gcatagcaac tgtattatat ttttaaagcc
    19621 aatggatatt cactcttatc actgtgaagg tttagtctgg gaacagtact ggatggaatt
    19681 tacccccacc agtatgatgg atatttccgt tggtcagcaa agcgttattt ataatggcga
    19741 aatttataat caggaactca ccgaagttgc tgagttaata acttcaccag aagcaataaa
    19801 aaataatctg gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgtc
    19861 cgcagatggt aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca
    19921 tgccagtctg caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga
    19981 agcctggttg cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct
    20041 cgatgcgcgt ctggatctgg cgctatcgct attaaagcaa caaggaaact cggttggcga
    20101 agtcgctgat acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaaacataa
    20161 atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa cggatgccag
    20221 tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca actcatccag
    20281 aatagctttg gcgaccgagg cgctcggtac cgttttcgac aacgtaatcg cctgccacag
    20341 gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg ccacttgctg
    20401 actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt caggccgtag
    20461 tagcgcacgc gcatctgttt gcgatcttgc agcccaacaa tttgcgccat ccggctttca
    20521 ataccgattg gcatgtcaca gatgttgagg attttcgcat tcgggcgcag acgacgcgtg
    20581 gcttctgcga caattgctgc cgggttggag tagttgagca tctaggcgtt tggtgaatat
    20641 ttttccatat aatccaccag ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt
    20701 ccgccgggtc cgcaagtttc ctgaccaaca acaccgtggc gcagtgggat tttttcatcc
    20761 agttcgcgca tcgggtactt gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg
    20821 aacgccactt caggatcggt ggtgtaacta aacgcaatgt ccggcgcttt ttctttaagg
    20881 atgactttac aggcttcggc aatcacttcc tgccgcgcgc catcgttatc ataaaatttc
    20941 agtgcacgaa gcgggaaacg gtcctgattc gccaggagca tcaacacgat gcctggcgta
    21001 aaggtgcttc caccgcctgc gacaaccact gagaatttgg tcattatact gcctccgtaa
    21061 tggcaacatt ttctgctgat tgatgagaat taattaagct atcgagctgt tcacgcaatt
    21121 gggatacatg cagaccaatg atcacctgaa tggcatcgcc actacggaag acgccgtgcg
    21181 ctcccagctt tttaaagact tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac
    21241 gtagacgcgt cgcgcaattg ttaatgctgg agatattgcc gaccccgccc agggcttgca
    21301 ggataccggc agcctgatcc agctcttttt ttggctctgc agcggtggtt tggcctcgcg
    21361 aggctttgta ttcggctttt gagtagagtt tcacttccgc atcttcacgt cccggcgtgc
    21421 acatgttgaa ctgcagaatc agggtgcgga aaaccacgaa gtacagcagg gtaaagcaca
    21481 acccaatggc gatttgggtc agcatcatat ccgcgtggtt gctgaacatc gggatccagt
    21541 tttgcggtaa aacctggtca atcagacctc cgcccatgtt gcccaccaca ccaaagagat
    21601 acattacggt cgacattgag gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa
    21661 tgaacaggaa ggtaaattcc agcggttcgg taattcccac cagcatggcg gttaaggttg
    21721 cgggaatcag caagcccgcc acttttaccc gattttccgg tgcggcagtg aagtacatcg
    21781 ctaaagaaat gcccacggca ccaaagattt ttgagttacc gtgcagggca aaaccgcctt
    21841 ccgggaacaa cgatttcagt ggctcggcgc tcagactgaa ctcttgcaga tgctgcgccc
    21901 agtacatctg aatgccgcct tcaacagctg ccggaccaaa gataaacggt ccgtagatga
    21961 agtggtgtaa accggttggg atcagaatac gttcgaggaa ggtgtaaacc cacaccccaa
    22021 gcgcacccgc cgaacgcagg aacgcttgca gagattcaat ccccatttgt acttttggcc
    22081 agccgagcaa tgtcagccag gcacagggga tcatcaccag gaaggcgata atcaccacat
    22141 aagacgtccc ctggaaaatg ccgagaaaaa ccggcagttt tttatcgaac agacggttat
    22201 gcagcgccgt cacaatgccg gaaatgataa ttgcgccgat aatgctggta tcgagggttt
    22261 taatcccggc catcattgtc agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc
    22321 cgaagtagct tccccaggtc attcccatcg cattgatgaa atagttccag gtcaggaaac
    22381 tcaccatcac cgccagacaa gcacgtccct gcgcttgctt agcaaggcca atgggtaaac
    22441 cgacagcaaa aatcagcggc atattacgga ataccgtcca accgccctct tcaataatgt
    22501 gtacgatttg cgcgaataaa ctgttcggat cggtcagtga ttccccgaca aacatcgggt
    22561 tttgcagcaa gatggcaaga cccaccacaa tcccggcgaa gggaaacagc agcactggcg
    22621 tgaacatcgc gccgccaaag cgttgaattt gactgagcat ttccacatcc tcttttctca
    22681 attctgtaag taggaagcct gattggttat gcggcagacc tggagcgagg ataggaagat
    22741 gcgatcggca gaacatgacg acccaatggg attcgtgatc gctttcatgc ttttcgttgg
    22801 gcggtagaga tctacgtttt cgcgaaaaag tagacatctg tgagcacagg tacacagcat
    22861 cgaacacatc tttaaaaaaa agattttttt caatcgatta agcagaactt gtgggcgcat
    22921 tacccgtgct tgcaggcaaa aaagagagct agagatgatc tacaaaagca ttgcggagcg
    22981 gttaagaatt cgacttaatt ccgcggattt cacgctaaac agccttcttc ccggtgaaaa
    23041 aaagctggcg gaagagtttg cggtatcgcg gatgactatc cgtaaagcca ttgacctgct
    23101 ggtagcgtgg gggctggtgg tccgccgcca cggcagcggc acttacctgg tgcgcaaaga
    23161 tgtgctgcat caaaccgcca gcctgaccgg actggtggag gtgttaaaac ggcagggaaa
    23221 aacggtcacc agccaggtgc tgatttttga aatcatgcct gcgcctccgg ccattgccag
    23281 ccagttacgg attcaaatca acgagcagat ctacttctcc cgtcgcgttc gttttgtgga
    23341 agggaaaccg ctaatgctgg aagacagcta tatgccggta aaactgttcc gtaacctttc
    23401 gctgcaacat ctggaagggt cgaagtttga atatattgaa caagagtgcg ggattttgat
    23461 tggcggtaat tatgaaagcc tgatgccggt gctcgccgat agactgctgg cgcggcaaat
    23521 gaaggtagcg gaacacacgc cactgctgcg gatcacctcg ttgtcatata gcgagagcgg
    23581 ggagtttttg aattattcag tgatgttcag aaatgccagc gaatactagg tggagtacca
    23641 tttacggcga ctccacccgg aaaaaagtta accgatactc cagaagagca ccgccagtaa
    23701 ttggggggtg ataatgcgca ggaacatcac taacggatag acagtggcgt aagagagcgc
    23761 cgccgcacca ctggttggat gaagattatt agcaaacgcc agcgccggag gatcggtcat
    23821 ggaacctgcc agcatcccgc acatggtcag gtaattcatt ttggctaaca tccgcgccag
    23881 aatgccaaca gtaatcagcg gaacggcggt gatcagggca ccataaccaa tccagcttag
    23941 cccttcgcca ttgaccaggg tattcacaaa atccccacca gatttcagac caacgaccga
    24001 gaggaacagc acgatcccca gctcccgcag cgcgaggttg gcacttggcg gcataaacca
    24061 gtacagcttg ccgatactgc cgatacgccc gaggatcaac gccataatca gcggaccgcc
    24121 cgccagcccc agtttcaacg cggccgggaa tcctggcaca aagacgggaa tagaacctaa
    24181 caatacgcca agcccgatgc caataaacac tggcagcatc tgaacctgtt gcagtttttg
    24241 ctgcgcattc cccagcacat tggcaacggc atcaattgcg gacggacgcc ccaccaggtt
    24301 gaggatatcg ccgaactgca ggctgatatc gccactggcg accagttcga ccccggcacg
    24361 gttcaggcgc gagataacaa cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt
    24421 tccgagcacg ttttcattgg tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt
    24481 agacagcgag gtatcgacct cctgaccaat caccagttgc gcattatgta aatccgctgg
    24541 ctgccccacc agatgcagca aatcgcccag ttggataatg gtatctggcg aaggtacttt
    24601 tagggtttct tctcgtttca gacgcgagca gataattttg tcgccgttga gaatcggtac
    24661 atctttaatg gctaaatcat gcaggttagg gttctcaacg cgaatattga tagtcttgat
    24721 cagcgcgccg ccattggtgc gtgaagactc gtgctgctga gcttctgtct cgacattgac
    24781 gcggaaaata acccgcaaca tccacatggt gaacaaaatc ccgcaaatgc cgaatggata
    24841 cgccatcgcg taactcatcc ccatctgatc gaccatttcc attggtgtac ccaggtcgcg
    24901 caagatctgc tgccctgccc ccagcgctgg cgtattggtt accgcaccgg agaaaatccc
    24961 cagcactacc ggcagtggaa tatcaaacag tttatgcagg atggcggtaa ccagaccacc
    25021 gatgatgacg atcagaacag caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc
    25081 aaagaagccc ggccctacct gaatcccgat ggtataaacg aacaggatca ggccaaattc
    25141 ctgaataaca tgcagcatat cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc
    25201 gacgatgatc ccaccaaaca gcacgccgcc aatacctaat cctatgccgc gaaatttgac
    25261 gttgccgata aacaaaccga cgactgccac caaagccaga atactgaccg ttaatgctat
    25321 atcactcatc gtccatttct cttgagataa cattttaatt gctacggatt atggctgaat
    25381 cggtgaagca gatttgcctt atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc
    25441 ttaaacagtc gaaatttaac gcattgttga agaagaaaga caaaaaaagc cccaccagaa
    25501 tggcggggca aagagaatag ctagttagct atttaacgcg ggacgttcgc tgatagcgat
    25561 acgctgcgct gcgatgggtt caggctcatt acgaattaaa tcaatatgca gtaaaccgtt
    25621 tacgaaggtt gcgccagaga cttccatatt ttcagccagc gtaaaactca ggctaaatgg
    25681 ctgattcata agcccttgat gcagccattt tttctcttct tttggctgct ccggcgtgcc
    25741 ttttacgctc aggcgcgtgc cttccagttg aatctctaaa tcttcctgac ggaaacctgc
    25801 cagcgcaagg gtaatgcggt agtggttatc gtcgcttttc tcaatgttgt acggcgggaa
    25861 gctctggctt tcaccggcgt tttgcagtgc gttggccagt ttgtcaaaac cgatccattg
    25921 acgcatcagt ggggataaat cgaagttacg catagtcatt tctccttcta agaagcgagt
    25981 aagtacctgc aaatccgaag attcgcatat gctccctgac ggcgagcatg gagatgtcag
    26041 gccgcgccag gcggccttag ggaattagtt gatttcgata cggcgcggtt ttttcgcttc
    26101 cggaatcacg cgttcgagat cgatatacag caaaccattt accaggttag ccccacgaac
    26161 atgaatgttc tcagctaact ggaatttgcg ttcaaagttg cgttcagcga tgccctggta
    26221 cagataggtg cgctcttttt gttcgtcggc gtgagcacct ttcaccacca gcagattatc
    26281 ctgggcggta atttccagtt cgctctcagc aaaaccagcc acagcgatag caatgcggta
    26341 atggttttcg tctaccagtt caacgttata cggagggtag ccgccattac tctggctctg
    26401 gttgttttct aagtggttaa acaaacggtc aaatccaata gcagaacggt aaagcgggga
    26461 taaatcaaag ttacgcataa tcaatagctc ctgaaatcag cgagaatgta agaccttcca
    26521 caatggacag gtcaggtagc cagaacaccc atcaggcgcg tcctcatcga ctacgatgta
    26581 aaatgggtct ggaaatgaac ttttcaagcc ttatcagcgg actttttttt gcagtttatg
    26641 gtctattgca tagactgagg gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg
    26701 cgacgtaatg atgatgttat tttcagcaag ggatcgctat aactcatcag gccaacaccc
    26761 acgatcaaca gatgaattta ttatgataag aaatgtgttg ttagcgttca tgatatgcag
    26821 cggaatgaca ttactcggag gatgctccag cgtgatgtcc cataccgggg ggaaagaagg
    26881 aacgtatccc ggcacccgcg ccagcgccac catgattggc gacgatgaaa ccaactgggg
    26941 cactaagtcg ctggcgattc tcgatatgcc gtttaccgct gtaatggaca cgcttttact
    27001 gccgtgggat gtgttccgca aagacagctc ggtgcgctcg cgcgttgaaa aaagcgaagc
    27061 caacgcgcaa gccaccaatg cggttattcc accggccaga atgcctgata actgatttag
    27121 cgatcagttt cggtgatcca cagctggcca ccttccatcc acgccagcca ttgaccatcc
    27181 ggcgagaaga ccacggcgtc cgcagaaggt ggattggcgt ggttttcggt taaatactca
    27241 acctcgccgc tttgcgcatg ggcacaaaca attcgattat ccagcacaaa gcccaaccat
    27301 tctcctgacg gatgccagtt aaatgcagac tgaatatccg ttttgttatg ggttaacttg
    27361 cgcggttcgc cgccctgtgg cgagataagc cacagttgca caatgccgtt atcatcacgc
    27421 attaaaaacg cgatttgcgt accctgcgga ttacagcgca cccagtggcg cgggacgttg
    27481 actaaccccg gataagcccg atgatgggta aaggttaaac gtcgctgcac gacgccacgc
    27541 ggtggcgcgg gtagggttgt ttccgttcca cttaacggcg catcacctgc cgatttccag
    27601 ctagcttcat cttgcggtaa ctcaacgata aatagctccg gcactttctc gccctttggc
    27661 gaaagtgtgt cgccaataaa cgccagcgcg tgatttccta cccatccttc ttcataagca
    27721 cgattgattt catcactgcc aggctgcggc gtgggcgtgg ttttactcac cagcacacac
    27781 cagtggctac cgctgtattc acgcggatgt tgcttttgta cgttgaccgg gccaaacggt
    27841 gcagcaaccc cgacgtttcg caaatccagc gccggatcga gttgatgcat tacatggtcg
    27901 ttataggtaa agctccccct ttcaccgttc gggctaaaga catgcacatg gctgccaccg
    27961 cgcagcgctc ctggcgtata cggcgcggta atatccattg catcgagatt gctcaccttg
    28021 ccgccttcag caatgactcc gcgtcgatga tggaagtcgt aatgccatgt ttcatcagga
    28081 ttttcagggc cgtgaataaa gacatatttc tctgacttcg gatgaacggt caccacgccg
    28141 acgtgtgcgc cctgtgacgc gcgatagata acctcgacct cgcctgtatg gatattcaca
    28201 cgctcaatgg tctcgccggt aaacgacgcc ccagaaggac gcacgtcaaa taccagccac
    28261 tggctgtcgg gcgtccaggt attggtattt gtattggtat tggtattggt gagcaggtga
    28321 ttacggggag caaaggttat ctgtttcatg gcgcggtcct gatacaaaac ggttcccgcc
    28381 atcatacttc acaaggactt cactttcaga caatagtctt ttgccatcgt gatgaatgtg
    28441 agtgaaaaga tggaacattt cgacgtggcg attattggcc tcggcccggc agggtcggcg
    28501 ttggcacgaa agttagccgg caaaatgcag gtgatcgcgc tggataaaaa gcaccagtgc
    28561 ggtactgaag gtttcagcaa accttgcggc ggtctgctgg caccggacgc gcagcgatct
    28621 tttattcgcg atggactgac gcttcctgtc gatgtgatcg ccaatccaca gattttcagc
    28681 gtcaaaactg tcgacgtcgc cgcatcgctc acgcgtaact accagcgaag ctatatcaat
    28741 attaatcgcc atgctttcga cttgtggatg aaatcgctga tccccgccag cgttgaggtt
    28801 tatcacgata gcctgtgccg aaaaatctgg cgtgaggatg ataaatggca tgtcattttt
    28861 cgtgcagacg gctgggagca acatattacc gcccgctatc tggtcggtgc cgatggcgca
    28921 aactcgatgg tgcggcgaca tctctacccg gatcatcaaa tccgtaaata tgtcgctatc
    28981 cagcagtggt tcgcagagaa acatccggtg ccgttctact cctgcatctt tgataatgcg
    29041 ataactgact gttactcatg gagtatcagc aaagatggtt attttatctt tggcggtgcc
    29101 tatccaatga aagacggtca gacgcgtttc acgacgctga aagagaaaat gagcgccttt
    29161 cagttccagt ttggcaaagc ggtgaaaagt gagaaatgca cggtgttgtt tccctcacgc
    29221 tggcaggatt ttgtctgtgg taaggacaac gcctttctga ttggtgaagc ggcgggattt
    29281 atcagcgcca gctcgctgga ggggattagc tatgcgctgg atagcgcaga gattctgcgt
    29341 gcggtgttcc tgaaacagcc ggagaagagc aacgccgctt actggcgcgc cacacacaaa
    29401 ctgcgtttaa aactcttcgg caagatagta aaaagccgat gcctgaccgc accggcttta
    29461 agaaagtgga ttatgcgcag tggtgtggcg catattccac agttgaaaga ttatccaacg
    29521 cgcttcacat cacccaccag caggatgtaa gagagcgcgc caatcaacgc gacggcggag
    29581 atataaacca gtgcaggcgc gaaaccgtaa ccctgcgcca ggtaccccac caccagcgga
    29641 acggtgatgc cgcccagacc accggcgaag ttaaacacgc cgccggttaa accaatcagg
    29701 cgcatcggtg ccagagaaga gaccagcgac caggtaatcg aagcaaaacc gttgccgaag
    29761 aatgccagcg ccatcaggca cataatcatc atcggatcgt tggtgtagtt agcgcccata
    29821 atgcaggtgg agatcagcaa gccgcagata atcggcgttt tacgcgcaaa gcccagtgaa
    29881 aagcccttac gtaccagcag atccgcgacc cagccagaga gcaggacgcc gacaaacgcc
    29941 gcgaggaatg gcaccgtggt cataaagcct gctttcagcg ccgtgattcc tttttcctgg
    30001 gttaaatagt tcgggaacca ggttaagaaa aaccacagtg cagaagccac cgcaaattgc
    30061 ccaagataaa cgccgatcag tttacgatgg aacaccagtt tccagtcttt ggctgttaac
    30121 ggctgacgcg cctctttctt caccggcgca tcaccatcca ccagaccgcc gccatcacga
    30181 atgtaatcca gttcagcttt gctgatacct ttggtcaggc gcggcggctg ataaacctta
    30241 aaccaaatca gcgaccagat aatgccgata ccaccagtga caatgaacac ccagtgccag
    30301 ctcaacatct cctgaatcca gatcagcagc ggagtcaaaa acgccagacc gacaaactga
    30361 ccagacgtat aaaaaccaac ggcggaagcg cgttcatgtt ccgggaacca gctggtcacc
    30421 atccggttat tggtcgggaa agcgggcgct tcgaaaatac cggttatcgc acgcagacca
    30481 attaatgaca ttaatcccgt ggcaaagcct tggaataaag tcgccactga ccaaccaaat
    30541 atcgcaataa aataagtcac gcgagaacct acgcgatcta aaaaccaacc gccggggatc
    30601 tggcatagcg tataaagcca ggcgaaggcc gaaaatacat agcccatttc cgctttcgta
    30661 atgccgaact cttcctgaat atgggcggaa gccacggcca ggttagcgcg gtcaacataa
    30721 caaatgacca ccgtaataaa gatcatcacc agcgtcagat aacgccgacg ccccggcttt
    30781 gctgcattaa cgggaatatc catagcgagc tttctccaga ttttgggcat agcgaagccg
    30841 ctcaccatgc cctgtaattt acagagggtt atatttttgt attgctgttt tagtgcccga
    30901 tgaggggctt acgtggcagg aattaccact ctgctacgct gttatcttca tgacgccaga
    30961 gcggattacg ccagtccggg gcatttttac tgaactcaat aactttagct tcgtcgattt
    31021 ccacacctaa gcccggtttc gttaacggtt taaagaagcc gccaaccatg ctgaagtctt
    31081 ctttgttttt cacaaagtcg agtaactccg cgcctttgtt gtaatgaatg cccatacttt
    31141 gttactgaag taccgcgtta taggaaacga agtcgatatg caggcaagcc gccagtgcaa
    31201 tcggtccgaa cggacagtgc ggcgcaaggg tcacatcata ggcttctgcc attccagcaa
    31261 ttttgtagca ttcggtaata ccgcctgcat gggagagatc cggttgcaga atcgaaatac
    31321 cgcctgcctc cagcacgcgt ttaaaatcga aacgtgagaa catgcgctca cccgccgcca
    31381 gtggaatatg cgtttgtgcc gccaatttcg ggtagtattc ggcttgttcc gccagcaccg
    31441 gctcttcaat aaacagcggg cgatacggct ccagttcttt aatcagcact ttcgccattg
    31501 gcgcgctgac gcgaccgtgg aaatcaagac caaactcaat ctgattgcca aaagcttcac
    31561 gaatttgtgc cacggtgttg actgccgcat ctaccgcgcg ggagttatca attagcccca
    31621 gttcttcaca accgttcagt ttgaaggtat cgaagccgat ttcgcgcagg gttttaatgc
    31681 cgtcgataac atccgccgga cgatcgccgc cgacccaact gtaggcttta attttgtcgc
    31741 gaaccaggcc gcccatcagt tgccagactg gcgcattcag cactttgcct ttgatatccc
    31801 ataacgcctg gtcgatcccg gcgatggcgc tcatcaggat tggaccgcca cgataaaatc
    31861 cggcgcgata catcacttgc cataagtcgt tgatgcgcga aggatcctga ccaatcaaat
    31921 agtcacccag ctcgtgaact gccgcttcca ccgtacgggc gcggccttca atcacgggct
    31981 cgccccaacc gaccacgcct tcatcggttt caattttcag gaacatccag cggggaggta
    32041 aacgatacgt ggtaattttg gtgattttca ttgcactgcc tctcgatacg ccttaacaaa
    32101 tgctgctgcc tgctgcgcgg tgcgttctac ggactgcccg gcgcgataaa gatcgctgcc
    32161 taagcccgcc ccaacacaac ctgcgtctat ccactgcgcc aggttttctg gcgtcacgcc
    32221 gccaacggca aagactgcga tgtccggtgg taataccgct tttaacgctt tgatgtattg
    32281 cggaccaaaa gccgatgacg gaaatatttt cagcgcctgc gcgcccgctt cgagcgcggt
    32341 aaaggcttcg gtcgccgtcg cgcagccggg gcaaacggtc atgccgtagc ccaccgcgcg
    32401 gcggatcact tcactatgga tattgggcgt aacgatgagc tgacagccca tcctggcgag
    32461 cgcatcgacc tgttcaggtt tcagtaccgt acctgcgcca atcaacgcct tgtcgccata
    32521 cgcatcaacg atggcgggaa tgctctgctc ccattgtggg gaattcagcg ggatttcaac
    32581 cgcgtcgaac ccggcgtcaa tcaccgcgcc aacatgcgcc agcgcctcgt cgggcgtaat
    32641 gccgcgcaaa atggcgatca gcgggagttt agtttgccac tgcatgagcg atgctcctta
    32701 taccagcctg aaatgccgtg tcgcccgcca ccgccgccac gtcgcaaccc atcgcctgaa
    32761 aggcttgctg gtagcgcgcg gtcagcgatg ttccggcgac aagggtgatg gcgtgttgat
    32821 gggtcacata gtcgcgcata ctggcaacct ctgcgccaat caacaaacca gagagaaatt
    32881 cgctgacctg ttcgcgggga agtgttccca gcacatgcga ggcgcgaact tcaaaaagct
    32941 gcggcaatat ggcgggcgca ttaaggccgc gctcaaggcc agccgcgaag gcatcggcag
    33001 agttttcctg cggcggcaaa cctgcgccaa tcaatgagtg atttaacagt aaatgatgta
    33061 attcaccggt catcacggtg cgaaaatcgt tgatttgctg gctatcggcc tgcacccatt
    33121 tgcaatgcgt accgggcata acataaagag aggaaggagc cagagctcgc gcgccgatca
    33181 attgtgtttc ttcgccgcgc atcacattgt ggttatcgtc atgagagacg cataatccgg
    33241 gaataatcca gatattgtcg ccaacagacg ttaattgttc gccaatagac gaaaaacggg
    33301 caggaacaga taaatacgga gcaactttcc agccgacatt gctgccgacc attcctgcca
    33361 tcaccactgg cgtattttct tcacgccagt cggtcgtgac ttctgctaac actgcagccg
    33421 gagattttcc gttcaggcgc gtgacgcctg cttctgattg cctgctctcc aggcagtggt
    33481 cgccctgata aagccaggcg cgcagattgg tcgatcccca gtcaattgcg atgtagcgag
    33541 ctgtcatgtg atttccttta accttcgtgt cgagctggca atcatggtaa gcgccgcctg
    33601 ctctgccgca tcgccgtcct gatgccgtat cgcatcgaac agcgccttat gttcctggag
    33661 cgtttgcggc atgttggcct catcgcccat ccaggttcgt tcaaaaaccg cccgctgcag
    33721 cgaactgatc gcaatgctaa gttgctgtaa caccgggtta tgcaccgact gcagcaccgc
    33781 ctcgtggtag cgaatatccg cttcgttaaa cgcttcgcgg tcctgattgt tggcaatcat
    33841 ctcgttcagc gccgattcaa tctgcgccag atcgctggag gtcgcgcgct ctgctgccca
    33901 acgggcaatc gccggttcca ccagatttcg cacttcgctc atggcactga taagccgtgg
    33961 gtcgtagtcg ttttccagca cccattgcag tacgtcagtg tcgaggtaat tccactggtt
    34021 acgcggtgcc acaaacgccc cgcgataacg tttcatttca atcagccgct tcgccatcag
    34081 cgaacggaac acctcacgga tgatgttgcg cgaggttgca aactcttcac agagctccgc
    34141 ctcagccgca agtggcgagc caggtacgta tttgccgtga acgatctgtt tacccagcgt
    34201 aatgacaatg cgatcggttt tattgagagt catggagagt ccttgtgctc tgtatgttct
    34261 tctctacttt accccgatcg atgcataacg cggcaacttt gtagtaccag cgtgatgacg
    34321 ttcgcgtttg ccgcgcgcgt aatgtagtac aaacttatat tgttgcgcta caatttagat
    34381 caaaaaaaga acaacacata aaaaaatgac atgcgtcggg cagaaatctg aaaagggata
    34441 tcaggcgcta aacaggaggg aaagaagagt atgctttcaa cggcttagct actcgtttaa
    34501 aggattaatc atgaagttga attttaaggg attttttaag gctgccggtt tattcccact
    34561 ggcgctgatg ctttcaggct gtatctcgta tgctctggtt tcccataccg caaagggtag
    34621 ttcaggaaag tatcaatcgc agtcagacac catcactggg ctatcgcagg caaaagatag
    34681 taatggaaca aaaggctatg tttttgtagg ggaatcgctg gattacctta tcactgatgg
    34741 tgccgatgac atcgttaaga tgctcaatga tccagcactt aaccggcaca atattcaggt
    34801 tgccgatgac gcaagatttg ttttaaatgc ggggaaaaag aaatttaccg gcacaatatc
    34861 gctttactac tactggaata acgaagaaga aaaggcactg gcaacgcatt atggttttgc
    34921 ctgtggtgtt caacactgta ccaggtcact ggaaaaccta aaaggcacaa tccatgagaa
    34981 aaataaaaac atggattact caaaggtgat ggcgttctac catccattta aagtgcgatt
    35041 ttatgaatac tattcaccca gaggcattcc ggatggtgtt tccgcagcat tactgccagt
    35101 gactgttaca ctggacatca ttactgcacc gctgcaattt ctggttgtat atgcagtaaa
    35161 ccaataatca gtaagcgggc aaacgcgttt atgctgtttg cccgcccaca gattaattca
    35221 gcacatactt ctcaatagca aacgccacgc catcttcaag gttggatttt gtgacaaagt
    35281 tcgccacttc tttcaccgaa ggaatagcgt tatccatcgc cacaccgacg cctgcatatt
    35341 caatcattgc gatgtcgttt tcctgatcgc caatcgccat gatttcttcc ggtttaatac
    35401 ctaacacgtc ggccagtgat ttcacacccg tacctttgtt aacgcgttta tcgaggattt
    35461 cgaggaagta cggcgcactt ttcagcacgg tatatttctc tttcacttcc tgcggaatac
    35521 gcgcgatagc ctggtcgagg atggcgggtt catcaatcat catcactttc aggaactggg
    35581 tattggggtc cattttctcc gcttcgcaga acaccagcgg aattgtggca acgaaggatt
    35641 catgcaccgt gtagtagctg atatcgcggt tagcggtgta cagcgtggtg cggtccaggg
    35701 cgtggaaatg agaaccgact tcgcgagaga gtttttccag gaaacgatag tcgtcatagc
    35761 tgagagcggt ttgcgccacg gtgctaccat cagcggcctt ctgtaccagc gcgccgttat
    35821 aagtaatgca gtagtcgccc ggctgttcca tatgcagctc tttcaggtag ttgtgcacac
    35881 ctgcatacgg acgacccgtc gttagcacga cattcacgcc acgggcgcga gctgcggcaa
    35941 tcgcattttt aacggcgggt gaaatggtgt gatcgggcag cagaagggtg ccatccatat
    36001 cgatagcaat gagtttgata gccatgagtt cccaaggtag attagttcct gacccatgct
    36061 aacgcgattc cgctcaaaaa tcagtacaac acccgaggga aaaggggatg caacgcgggt
    36121 tgcatgctcc ccatttgctt agcggaagag tttccctttc agcagttcca tgcctgcgga
    36181 aagcagatcg ttattggctt gtgctgacac ttcaccttgc ggtgagagcg catcaataat
    36241 cttcggcaat tgctctgcca gtaaactgga agccgtactg gtatccacgc caagtttttg
    36301 cccgagatcg gacaccgcat ttgtgccgag cgccgactcc agttgctcgc cgctaactga
    36361 ttgatttctc tgttgattac tcaaccaggt tgagagaatg gcccctaagc cgccactttg
    36421 cagtttttcc agcagcacct gaatgccacc ctgctcctca acccaactta aaatcgcctg
    36481 atatttcccc gcatcgcctt tcagaaaggc accgacaact tcatcaaaaa gccccatgat
    36541 aatcacctgt aaagcgttac gtgttgaccc aaaaagtata gatctgcggg atgataattg
    36601 cggattgcag aaataaaaag ggcggagatg atctccgccc ttttcttata gcttcttgcc
    36661 ggatgcggcg tgaacgcctt acccggccta caaaatcatg caaaatcaat atattgcagg
    36721 agtctcgtag gactaataag cgtagcgcat caggcacgct cgcatggtta acgccattaa
    36781 atatcaatat tcgccgcttt cagggcgttc tcttcaataa acgcacgacg cggttctacg
    36841 gcgtcgccca tcagcgtggt gaacaactgg tcagcggcaa tcgcatcctt aacggtaacg
    36901 cgcagcatac gacggctttc cgggtccatg gtggtttccc acagctgttc cgggttcatc
    36961 tcgcccagac ctttataacg ctggatggag aggccacgac gggactcttt caccagccag
    37021 tccagcgcct gctcgaagct ggctaccggc tgacgacgct cgccacgttc gataaacgca
    37081 tcttcttcca gcaagccacg cagtttctca cccagcgtgc agatacgacg atattcgcca
    37141 ccggtgataa actcgtgatc cagcggatag tcagtatcca caccgtgggt acgcacgcga
    37201 acaatcggct cgaacaggtt ttgttcggca ttggtgtgga catcaaactt ccactggctg
    37261 ccgtgctgtt ctttgtcgtt cagttcgctg accagcgcgt tcacccagcg ggtaacggtc
    37321 tgctcatcag aaaggtcagc ttccgtcaac gtcggctgat agataagctc tttcagcatt
    37381 gctttcggat aacgacgctc catgcggttg atcattttct gcgtcgcgtt gtactcagat
    37441 accagtttct ctaacgcttc gccagccaat gccggtgcac tggcgttggt gtgcagcgtt
    37501 gcgccgtcca gcgcgataga gatctggtac tgatccatcg cttcgtcgtc tttaatgtac
    37561 tgttcctgct tgcctttctt cactttgtac agcggcggct gagcgatgta gacgtgaccg
    37621 cgttcaacga tttccggcat ctgacgatag aagaaggtca acagcagcgt acgaatgtgc
    37681 gagccgtcga cgtccgcatc ggtcatgatg atgatgctgt gataacgcag tttgtccggg
    37741 ttgtactcgt cacgaccgat accacagcca agcgcggtga taagcgtcgc cacttcctga
    37801 gaagagagca tcttatcgaa gcgcgctttc tcgacgttaa ggattttacc cttcagcggc
    37861 agaatcgcct ggttcttgcg gttacgcccc tgcttcgcag agccgcccgc ggagtcccct
    37921 tccaccaggt acagttcgga aagcgccgga tcgcgttcct ggcagtctgc cagtttgccc
    37981 ggcaggccag ctaagtcgag cgcaccttta cggcgggtca tttcacgcgc gcgacgcgcc
    38041 gcttcacggg cacgggcagc atcgataatt ttgccaacca cgattttcgc atcagttggg
    38101 ttttccagca ggtattccgc cagcagttcg ttcatctgct gttcaaccgc cgatttcacc
    38161 tcagaagaaa ccagtttgtc tttggtctgg gaggagaatt tcgggtccgg cacttttacg
    38221 gaaacgaccg caatcaggcc ttcacgcgca tcgtcaccgg tggcgctgac tttggctttt
    38281 ttgctgtagc cttctttgtc catgtaggca ttcagggtac gggtcatcgc cgcacggaag
    38341 cctgccaggt gagtaccgcc gtcacgctgc ggaatgttgt tggtaaagca gtagatgttt
    38401 tcctggaagc catcgttcca ctgcagcgcc acttcaacgc caataccgtc tttttcggtg
    38461 gagaagtaga agatatttgg gtggatcggc gttttgttct tgttcagata ttcaacgaac
    38521 gctttgatgc cgccttcata gtggaagtgg tcttctttgc cgtcacgctt gtcgcgcaga
    38581 cgaatggaaa cgccggagtt gaggaacgac aactcacgca gacgtttcgc cagaatttca
    38641 tattcgaact cggtcacatt ggtgaaggtt tcgaggctgg gccagaaacg caccatggtg
    38701 ccggtttttt cagtctcgcc ggtaaccgcc agcggagcct gcggtacacc gtgttcgtag
    38761 atctgacggt gaattttacc ctcgcgctga ataaccagct ccagtttttg cgacagggcg
    38821 tttactaccg aaacaccaac gccgtgcaga ccgccggaca ctttatagga gttatcgtca
    38881 aatttaccgc ctgcgtgcag aacggtcatg atcacttccg ccgccgatac gccctcttcc
    38941 gggtgaatac cggtcggaat gccgcgcccg tcatcctgta cagagacaga gttatcggcg
    39001 tgaatggtga cgataatttc tttacagtga cccgcgagcg cttcgtcgat agcgttatct
    39061 accacctcga ataccatgtg gtgcagaccg gtgccgtcat ccgtgtcgcc gatatacata
    39121 cccgggcgct tacgcaccgc atccagccct ttcaggactt tgatactgga ggagtcataa
    39181 gaattcgaca tcaacgtttc tcgctcattt atacttgggt taatccgtta ttttaccctt
    39241 ttccacggta aacatcttcg aattttcgtc cgacatgtct ataacgtgtt cagcactgat
    39301 cgcgctgaca aagacctgtg attgcgtcgc ttttaagcgg ctggcaagca acccgcgacg
    39361 ctcatcatca agctcagagg caaaatcatc tatcaggtag agacaccgcc gcccgctttc
    39421 acgagtgagg aactctcctt gcgccagacg taaggcgcac atcaacagct taagctgccc
    39481 acgcgataag gtatcttcca ccggcgcacc gtcggcgcga atgcgtaagt ccgctttatg
    39541 cgggccatgc gcggtgtagg ttagctggcg atcgcgttca aaattacgtt ccagcacctc
    39601 agcgtactct gtctctttct cccagccgcg ctggaaagag aaagtcagag aaaactcagg
    39661 gagaaattgc ttacaggtat catccatatc ggccgcgata ccggcgctat actccgcgcg
    39721 ccaggtgctg atttgctccg ccagcgggat cagttcttta tcccacgggc gtagctgttc
    39781 gtaacgagtc acctggcgca gcgccgcatt gcgctgcttg agcaatcgct tgagattgct
    39841 ccaggcggtg aaaaatccag gttcgttgtg aaagcatccc cagtcgagga atgctcttct
    39901 gtatttgggg ccgccgttga gtaaagtaaa cccttctggc gttatcaact gcatcggcat
    39961 caggtgcgcc agttccgcga ctttatgccc gtccgtaccg tcaatacgga ctttgctgtc
    40021 gccctgtttg tctttggtta agccaatcgc cgtctcgcgc tcttcgccct gtaatcgccc
    40081 gtggagaaca aatgcctcct gctcatggcg aatgacgcga ccaatctgca aactgcgaaa
    40141 cgcccgacca tggccgagcg tatagatggc ttccagcacg ctggttttgc cactgccgtt
    40201 ggcacctacc agaaagttaa agccgggaga taaagcgaga tccgcggttt caatattgcg
    40261 gaaatcgcgg atcaacaagc gggtgaggga cattacagtc tcattggcat gacaacatag
    40321 gcagccgact gtgatgcggc atcttcaatc tgcacgctgg aaaccgaatc ggtcagcatc
    40381 atgcggacgt tttcgcattt cagcgcgttc agaacatcca gcacatagct gacgttgaag
    40441 ccgatttcca tctccgcacc gctataggta acgtcgagga tctcttccgc ttcttcctgt
    40501 tccgggttgt tggcagtaat tttcagctgg ttttcgctga catacaggcg cacaccgcgg
    40561 aatttctcgt tagagagaat cgccgcgcga gcaaacgcct gcttgagcag atcgcagcct
    40621 gcttccagat gtttgtccgg gttcttcggc agaacgcgac ggtaatccgg gaagcgacca
    40681 tccaccagtt tcgaggtgaa aataaagtcg ccaacgtggg cacgaatgtt attgctgcca
    40741 atctgcacac gcagcagatt gtcgccgccg tcgagcatac gcatcagttc aatcacgcct
    40801 ttacgcggta cgatcaccga atggcttggc aaagattgac caattggcat tgaacagacc
    40861 gccagacggt ggccgtcggt tgccacggtg cgcagttctt caccttcggt ttcaaacagc
    40921 ataccgttta agtaatagcg aacatcctga tgcgccatcg aaaactgggt tgcttcaatc
    40981 agacgcttca tcgtcgcctg cggcagggta aattcgactt cgctctgcca gtcatcgagg
    41041 ttcgggaaat ccgccgctgg cagggtagac agcgaaaaac ggctacgccc ggagcgcacc
    41101 agcatccgtt caccttccag ctgcacggca atttccgcgc cttcaggcag accacggcag
    41161 atatcaaaga atttgcgcgc cggaacggtc gtcgctcctg gctcgtgtgg ctgaaccagc
    41221 gcaacgcgtg ccaccatttc catctcgaga tcagtaccgg tcagcgacaa cgtaccgtca
    41281 gcaacctgta acagcagatt accgagaatc ggtagcgtag gacgaccacc taacggaccg
    41341 ctcacctgtt gtagcggttt taataaatgc tcacgttcta cggtaaattt cataggttta
    41401 cgatgacaat gttctgatta aatttgaaaa atcttctttg atatcgtggc tctcttcacg
    41461 caactgctcg atcttacggc aggcatgaag caccgtcgtg tggtcacgcc caccaaacgc
    41521 atcgccaatc tctggcagac tgtggttagt cagctctttc gctagcgcca tcgccatctg
    41581 gcgcggacgc gccaccgagc gggatcgacg cttggaaagg agatccgcga ctttgatctt
    41641 gtagtactcc gccaccgtct tctgaatatt gtcgatggtg accagttttt cctgcaatgc
    41701 cagcaagtcg cgcagcgcct cacgcacgaa gtcgatggtg atcgcccgtc cggtaaagtt
    41761 ggcgttagca ataacgcggt tcagcgcccc ttccagctca cgtacgttag atcgtagacg
    41821 cttggcgata aagaacgcca cttcacccgg caaacgaatg tcattttcgt cggccttttt
    41881 catcaggatc gccacacggg tttccagctc tggcggttcg atcgccacag tcagtcccca
    41941 accgaagcgg gatttcaaac gatcctcaac gccgttgatc tctttcggat agcgatccga
    42001 ggtgagaatg atctgttgat taccttccag cagggcgttg aaggtgtgga aaaactcttc
    42061 ctgagatcgt tctttattag caaaaaactg aatatcgtcg atcagcagtg catctacgga
    42121 acggtagtag cgtttaaact cttcgatcgc gttgttttgc agggctttaa ccatgtcctg
    42181 aacaaagcgc tcggagtgca tataaaccac tttggcattc ggcttgcgcg ccataatgcc
    42241 gttacccacc gcatgcagca ggtgagtttt acccagaccc gtgccgccat aaaggaacaa
    42301 cgggttatag gcaccgccag ggttatccgc cacctggcaa gccgccgcgc gcgccagttg
    42361 gttagattta ccttcaacga agttatcaaa cgtgtgtttg acgtttacgt tagaacgata
    42421 ggtcggttct gccggggccg ggacgttatc ccaacctgag cgcgtagaag gcgcagcacg
    42481 ttgcggctgc gtttgcgcca cctgtgcagg ggccgcgacg ttgctcgtca ctgccgcttg
    42541 tggcgtttgc gtcaccggtt tggtgccgac ttcaaaacgc agctgtgggg catccgctcc
    42601 gcagaaactg gttagcagtc cattgatatt attaaggtac ttgtcccgta cccaatcgag
    42661 gacaaaacgg tttggcgcgt acagggccag cgtgttatcg ctcagttccg cctgcaatgg
    42721 gcgtatccac atactgaatt ctgtggctgg taactcatcc tgcaatcggg caagacactg
    42781 ctgccaaagc gaaagtgaca cggcggactc cactcgaaca aaagtcgata atgactaagg
    42841 ctgaaacatt catgattgtt gacgtacgtc gaaaagaccc tgcttgaggg tgacgcacga
    42901 accgctgtct gcggttatat gcccgatcaa gatcctgcaa aacgatcggg accgcggatc
    42961 atagcctaaa ctgcgcaaga gatcttctgt ttctcacaga ttttccctat ttatccacag
    43021 gactttccag aactcgctaa gtgtaaacga tcctgccgcg aggcgggcac gatttacgcc
    43081 gcatattgga aaatttaatg accatagaca aaaattggct taatcgatct aataaagatc
    43141 caggacgatc cttgcgcttt acccatcagc ccgtataatc ctccacccgg cgcgccatgc
    43201 tggtttccac tggtgtgagg tcgtacattt tccctgcgaa aaggtgcgga aaagcgcggt
    43261 aaataaggaa agagaattga ctccggtgtg tacaattatt acaatccggc ctctttaatc
    43321 acccatggct tcggtgtcca tcgtttcatt tttcggcgga tatccaataa agccattgaa
    43381 tttattcaag tttaggtaga aatcgccatg aaacgcactt ttcaaccgtc tgtactgaag
    43441 cgcaaccgtt ctcacggctt ccgtgctcgt atggctacta aaaatggtcg tcaggttctg
    43501 gcacgtcgtc gtgctaaagg ccgcgctcgt ctgaccgttt ctaagtaata aagctaaccc
    43561 ctgagtggtt aagctcgcat ttcccaggga gttacgcttg ttaactccca gtcaattcac
    43621 attcgtcttc cagcagccac aacgggctgg cacgccgcaa attaccattc tcggccgcct
    43681 gaattcgctg gggcatcccc gtatcggtct tacagtcgcc aagaaaaacg ttcgacgcgc
    43741 ccatgaacgc aatcggatta aacgcctgac gcgtgaaagc ttccgtctgc gccaacatga
    43801 actcccggct atggatttcg tggtggtggc gaaaaaaggg gttgccgacc tcgataaccg
    43861 tgctctctcg gaagcgttgg aaaaattatg gcgccgccac tgtcgcctgg ctcgcgggtc
    43921 ctgatagccc tcattcgggt ctatcaacgc ctgattagtc cgctactcgg gccgcattgt
    43981 cgtttcactc caacctgttc aagctacgga attgaggcat tgcgcaggtt tggagtgata
    44041 aaaggcagtt ggttgacggt gaaacgcgta ttaaaatgcc accctttaca ccctggtggt
    44101 gacgatcccg tcccgcccgg accatttgat accagagaac actaacgatg gattcgcaac
    44161 gcaatctttt agtcatcgct ttgctgttcg tgtctttcat gatctggcaa gcctgggagc
    44221 aggataaaaa cccgcaacct caggcccaac agaccacgca gacaacgacc accgcagcgg
    44281 gtagcgccgc cgaccagggc gtaccggcca gtggccaggg gaaactgatc tcggttaaga
    44341 ccgacgtgct tgatctgacc atcaacaccc gtggtggtga tgttgagcaa gctctgctgc
    44401 ctgcttaccc gaaagagctg aactctaccc agccgttcca gctgttggaa acttcaccgc
    44461 agtttattta tcaggcacag agcggtctga ccggtcgtga tggcccggat aacccggcta
    44521 acggcccgcg tccgctgtat aacgttgaaa aagacgctta tgtgctggct gaaggtcaaa
    44581 acgaactgca ggtgccgatg acgtataccg acgcggcagg caacacgttt accaaaacgt
    44641 ttgtcctgaa acgtggtgat tacgctgtca acgtcaacta caacgtgcag aacgctggcg
    44701 agaaaccgct ggaaatctcc tcgtttggtc agttgaagca atccatcact ctgccaccgc
    44761 atctcgatac cggaagcagc aacttcgcac tgcacacctt ccgtggcgcg gcgtactcca
    44821 cgcctgacga gaagtatgag aaatacaagt tcgataccat tgccgataac gaaaacctga
    44881 acatctcttc gaaaggtggt tgggtggcga tgctgcaaca gtatttcgcg acggcgtgga
    44941 tcccgcataa cgacggtacc aacaacttct ataccgctaa tctgggtaac ggcatcgtcg
    45001 ctatcggcta taaatctcag ccggtactgg ttcagcctgg tcagactggc gcgatgaaca
    45061 gcaccctgtg ggttggcccg gaaatccagg acaaaatggc agctgttgct ccgcacctgg
    45121 atctgaccgt tgattacggt tggttgtggt tcatctctca gccgctgttc aaactgctga
    45181 aatggatcca tagctttgtg ggtaactggg gcttctccat tatcatcatc acctttatcg
    45241 ttcgtggcat catgtacccg ctgaccaaag cgcagtacac ctccatggcg aagatgcgta
    45301 tgctgcagcc gaagattcag gcaatgcgtg agcgtctggg cgatgacaaa cagcgtatca
    45361 gtcaggaaat gatggcgctg tacaaagctg agaaggttaa cccgctgggc ggctgcttcc
    45421 cgctgctgat ccagatgcca atcttcctgg cgttgtacta catgctgatg ggttccgttg
    45481 aactgcgtca ggcaccgttt gcactgtgga tccacgacct gtcggcacag gacccgtact
    45541 acatcctgcc gatcctgatg ggcgtaacga tgttcttcat tcagaagatg tcgccgacca
    45601 ctgtgaccga cccgatgcag cagaagagca tgacctttat gccggtcatc ttcaccgtgt
    45661 tcttcctgtg gttcccgtca ggtctggtac tgtactatat cgtcagcaac ctggtaacca
    45721 ttattcagca gcagctgatt taccgtggtc tggaaaaacg tggcctgcat agccgcgaga
    45781 agaaaaaatc ctgattcggt gagttttcgc taaaataagg gcggtcagtt gaccgccttt
    45841 tttcttttcg tagggcggat aagcaccgcg catccgccac acaaagcaac aggaacatca
    45901 tgagcgataa tgacactatc gtagcccagg ccacgcctcc gggacgtggc ggcgttggca
    45961 tcctgcgcat ctccggcttc aaagcccgtg aagttgccga aaccgtgctg ggtaaactgc
    46021 ctaagccgcg ctacgccgat tatcttccgt ttaaagacgc cgacggcagc gtgctcgatc
    46081 aggggattgc gctatggttc cctggcccga actcgttcac cggcgaagat gtgctggaac
    46141 tgcaaggtca tggcggtccg gtgatcctcg acctgctgtt aaaacgcatt ctgaccattc
    46201 ccggcctgcg gattgctcgc cctggtgagt tttccgaacg cgcgtttctt aacgataaac
    46261 ttgacttagc ccaggccgag gcgattgccg atcttatcga cgccagttcg gaacaggcgg
    46321 cccgttcggc acttaactcg ctgcaaggcg cattctccgc acgggttaat catctggtag
    46381 aagccctcac ccacttgcgc atttacgtcg aagcggcaat tgatttccct gatgaagaga
    46441 tcgatttcct ctccgacggc aaaattgaag cccagcttaa tgacgtgatg gccgatctcg
    46501 atgcagtgcg tgctgaagca cgtcagggta gtttgttgcg cgaagggatg aaagtagtga
    46561 ttgccggacg tcctaatgcc ggtaaatcga gcctgttaaa cgcgctggcg ggccgtgaag
    46621 cggcaatcgt aaccgatatc gccggaacca cgcgtgacgt gctgcgtgag catatccaca
    46681 ttgacggaat gccgctgcat atcatcgaca ctgccgggct acgtgaggcc agtgacgaag
    46741 tggaacgtat tggtatcgag cgcgcgtggc aggaaattga acaggccgac cgcgtgctgt
    46801 ttatggtcga tggcaccacg acagacgccg ttgatccggc agagatctgg ccggaattta
    46861 tcgcccgtct gcccgcgaaa ctgccgatca ccgtggtacg caataaagcc gatatcaccg
    46921 gcgaaacgct gggaatgagt gaagtgaacg gtcacgcgtt aattcgtctc tcggcaagga
    46981 ctggtgaagg cgtggacgtg ctgcgtaacc atctcaaaca gagcatgggc tttgacacca
    47041 atatggaagg cggcttcctg gcgcgccgtc gccacttaca ggcgctggaa caggcagcgg
    47101 agcatttgca gcaaggtaaa gcgcaactgt tgggagcctg ggcaggtgaa ctgctggcgg
    47161 aagagttgcg cctggcacag cagaacttaa gcgaaatcac cggggaattt acttcagacg
    47221 acctgctggg gcggattttc tccagcttct gtattggtaa gtaaccgcgc ttacgaagcc
    47281 gtattctgac tgtcagatgc ggcttcgctt cattgttagc actcctgtta tgcctcaacc
    47341 cttttttaaa cattaaaatt cttacgtaat ttataatctt taaaaaaagc atttaatact
    47401 gctccccgaa cgattgtgat tcgattcaca tttaaacaat ttcag
//
LOCUS       DAJTUP010000020        47286 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTUP010000020 DAJTUP010000000
VERSION     DAJTUP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47286
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.020"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OO468_001173"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OO468_001173"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR6258364.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO468_001174"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO468_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR6258365.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO468_001175"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO468_001175"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR6258366.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO468_001176"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO468_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR6258367.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO468_001177"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO468_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR6258368.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO468_001178"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO468_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR6258369.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO468_001179"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO468_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR6258370.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO468_001180"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO468_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR6258371.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO468_001181"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO468_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR6258372.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO468_001182"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO468_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR6258373.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO468_001183"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO468_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR6258374.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO468_001184"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO468_001184"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR6258375.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="OO468_001185"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="OO468_001185"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR6258376.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="OO468_001186"
     CDS             11231..12085
                     /locus_tag="OO468_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6258377.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="OO468_001187"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="OO468_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR6258378.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="OO468_001188"
     CDS             complement(13548..14687)
                     /locus_tag="OO468_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR6258379.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO468_001189"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO468_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR6258380.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="OO468_001190"
     CDS             15873..17234
                     /locus_tag="OO468_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR6258381.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="OO468_001191"
     CDS             17231..17749
                     /locus_tag="OO468_001191"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR6258382.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="OO468_001192"
     CDS             17749..18069
                     /locus_tag="OO468_001192"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR6258383.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="OO468_001193"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="OO468_001193"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR6258384.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20110
                     /locus_tag="OO468_001194"
                     /pseudo
     CDS             18888..20110
                     /locus_tag="OO468_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20207..20629
                     /locus_tag="OO468_001195"
     CDS             20207..20629
                     /locus_tag="OO468_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6258385.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20677..21549)
                     /locus_tag="OO468_001196"
     CDS             complement(20677..21549)
                     /locus_tag="OO468_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR6258386.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21561..22655)
                     /locus_tag="OO468_001197"
     CDS             complement(21561..22655)
                     /locus_tag="OO468_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6258387.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22688..23686)
                     /locus_tag="OO468_001198"
     CDS             complement(22688..23686)
                     /locus_tag="OO468_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6258388.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23711..25222)
                     /locus_tag="OO468_001199"
     CDS             complement(23711..25222)
                     /locus_tag="OO468_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6258389.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25245..26227)
                     /locus_tag="OO468_001200"
                     /pseudo
     CDS             complement(25245..26227)
                     /locus_tag="OO468_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26324..29425)
                     /locus_tag="OO468_001201"
     CDS             complement(26324..29425)
                     /locus_tag="OO468_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR6258390.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29432..29605)
                     /locus_tag="OO468_001202"
     CDS             complement(29432..29605)
                     /locus_tag="OO468_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258391.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29723..30916
                     /locus_tag="OO468_001203"
     CDS             29723..30916
                     /locus_tag="OO468_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR6258392.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31114..32367)
                     /gene="glyA"
                     /locus_tag="OO468_001204"
     CDS             complement(31114..32367)
                     /gene="glyA"
                     /locus_tag="OO468_001204"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR6258393.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32694..33884
                     /gene="hmpA"
                     /locus_tag="OO468_001205"
     CDS             32694..33884
                     /gene="hmpA"
                     /locus_tag="OO468_001205"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR6258394.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33929..34267)
                     /gene="glnB"
                     /locus_tag="OO468_001206"
     CDS             complement(33929..34267)
                     /gene="glnB"
                     /locus_tag="OO468_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR6258395.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34328..35662)
                     /gene="glrR"
                     /locus_tag="OO468_001207"
     CDS             complement(34328..35662)
                     /gene="glrR"
                     /locus_tag="OO468_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR6258396.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35652..36365)
                     /gene="qseG"
                     /locus_tag="OO468_001208"
     CDS             complement(35652..36365)
                     /gene="qseG"
                     /locus_tag="OO468_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR6258397.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36530..37957)
                     /gene="qseE"
                     /locus_tag="OO468_001209"
     CDS             complement(36530..37957)
                     /gene="qseE"
                     /locus_tag="OO468_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR6258398.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38515..42402)
                     /gene="purL"
                     /locus_tag="OO468_001210"
                     /gene_synonym="purI"
     CDS             complement(38515..42402)
                     /gene="purL"
                     /locus_tag="OO468_001210"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR6258399.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42661..44217
                     /gene="mltF"
                     /locus_tag="OO468_001211"
     CDS             42661..44217
                     /gene="mltF"
                     /locus_tag="OO468_001211"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR6258400.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44214..44750)
                     /gene="tadA"
                     /locus_tag="OO468_001212"
     CDS             complement(44214..44750)
                     /gene="tadA"
                     /locus_tag="OO468_001212"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR6258401.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44775..45410)
                     /gene="yfhb"
                     /locus_tag="OO468_001213"
     CDS             complement(44775..45410)
                     /gene="yfhb"
                     /locus_tag="OO468_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR6258402.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45619..46467
                     /locus_tag="OO468_001214"
     CDS             45619..46467
                     /locus_tag="OO468_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR6258403.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46806..47286)
                     /locus_tag="OO468_001215"
                     /pseudo
     CDS             complement(46806..47286)
                     /locus_tag="OO468_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11272 a        12958 c        11895 g        11161 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtatattaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagcgc agcgcagcgc agcgcagatg taaggtgaca gtgattgaac tggcggcaac
    19441 cgtcatgggc cgtaatgcac caccgcccgt gcaacgctat cttttacagc gccatcagca
    19501 ggctggtgtg cgcattctgc tcaataatgc cattgaacat gtggtcgatg gtgaaaaagt
    19561 agaactgacg ctgcaaagtg gggaaacgct tcaggctgat gtggtgattt acggtattgg
    19621 tatcagcgcc aacgagcaac tggctcgcga ggccaacctt gatactgcca atggcattgt
    19681 cattgatgag gcttgccgca cctgcgatcc cgcgatcttt gccggtggcg atgtggcaat
    19741 cactcgtctt gataatggtg cactacaccg ctgcgaaagc tgggaaaacg ccaataacca
    19801 ggcgcaaatt gccgctgccg caatgttagg gctaccgcta ccgctactgc cgccgccgtg
    19861 gttctggagc gatcagtaca gtgataactt acagtttatt ggcgatatgc gtggcgatga
    19921 ctggctttgt cgtggcaacc cggaaactca gaaggcgatt tggtttaatc tgcaaaacgg
    19981 cgtgcttatc ggtgcggtca cgctgaatca gggccgtgag attcgcccaa ttcgcaaatg
    20041 gatccagagc ggcaaaacgt ttgatgcgaa actgctgata gatgagaaca tcgcgcttaa
    20101 atcactgtaa tcaggataat tagcgaatat ctcaatgcct ggggcgtggc gaggtgcaag
    20161 agtgtgtatt acgtttaaat cacattatct tgcaaaggga ttggttatga acacactacg
    20221 ttattttgat tttggagctg cccgccccgt tttgttatta attgcccgta tcgccgtggt
    20281 cttaattttc attatttttg gttttcccaa aatgatgggc tttgacggta cggtccaata
    20341 tatggcctcg ttgggcgcgc caatgccgat gctggcagcg attattgcgg tagttatgga
    20401 agtgcccgcc gcgatattaa tcgtgcttgg ctttttcacc cgtccgctgg cggtgctgtt
    20461 tattttctac acgctgggta cggcggtgat tggtcaccat tactgggata tgaccggcga
    20521 tgcggttggg ccaaatatga ttaatttctg gaagaatgtc agtatcgctg gcgcgttctt
    20581 gctattggca attaccggac cgggggcaat ttctctcgat cggcgttagg aaagatgccg
    20641 gatgcggcgt gaacgcctta tccggcattt tataaattac agccactcta cccgcaacga
    20701 catctctgag gttgttgatt cccctggtgc tagcgcaatg agatcacccc cttccgggcg
    20761 atgatgctca tccggcgcat ggctcatcgg ttccagacag aagaaatcaa acgcatatcc
    20821 tttatcaaac gcagggtcgg aaacaaagat gaaataacac ggtgcaggtg gcgtcgtttc
    20881 catgatgatg gcataacctt cctgcggctg ctcgatgcgg gcctgaccat tccatccggc
    20941 aaaaccattg ttcacccact gacgcggcaa cggcgcgagc tggttaaaat ccagttcctg
    21001 cggtagctgc tcgcaaaact cacccgccag ccactgctcc cgttccagcc agtaaccgct
    21061 cgcctgcgcc tgaatccgcg tttgtggtga caacggaaaa taaggatgcc agccggtacc
    21121 aaatggcagc gtctctgccc cttgattggt gacagagagc gtcaccgtca gcgtatccgc
    21181 cgttaaatga aacgcctgac ttacccgata gtgatagaca ccgctgcgat gttcatacac
    21241 cagacacaaa ctatcatcgc tgtgcgagac acattgccac tcgcccagcc agccatcgcc
    21301 gtgtagatag tgcgcatccc actcaacatt cggttgcagt tgatactcac gcccctgcca
    21361 gacaaaacgg ttgccgctca cccggtttgc aaatggcacc agcggaaaac atgaggcatc
    21421 ggttgccaca ccgctttttt taccaggacg taagagcggc gtcgtatcgc gccagaagcc
    21481 ttcgattacg ccgccctggt cagaaacgtc cagctttagc gacccgtggg ataaggtata
    21541 gatggtcatc cgctaactcc ttaatccggg aagttaatca caactttccc gcatttgccg
    21601 ctcgccatca gcacataggc atcacctgcc tgttctagcg agaagcgatg ggtaatggcg
    21661 ttacgcggcc acagcttcca gtcagtcaaa tcgtgggcgc atttttccat atggaacaga
    21721 ctggtcaccc aggagccgat aatccgccgt tgatggtgca tcagatcagc gctgacctcg
    21781 aattccactt ttccggtttc accaatgtaa accacccgcc cccagtcagc agtggattgc
    21841 agtgccagca agcgacctgc ggcattaccg gaacaatcga gcgcaacatc cgcgccaccg
    21901 tgggtaagtt cagcaattat ctgcggcaga ccttcggtgg ttgctaaata gccgtgatcc
    21961 atccccccta actgttttgc catcgccaga cgttccggca gcatatcaac gccgatgatc
    22021 cgttttgcac cgcgaccttt cgccagcatc atcgccatca tgccgaccgg ccccagaccg
    22081 accaccagca cgttatcgct gccggaaact tcgccgcgca aaatcccttc ataggccgtg
    22141 ccaacgccgc aactgataaa cgcaccatct tcgtagctca gcgcatccgg caggaggatc
    22201 agatcttttt cttccgccag cagatattcg gcatgaccgc cgtcacgctg ccagccgtaa
    22261 gccgcttttc ctttgccagt acaagagata ggaaagccgc ggcggcagtt cgggcaaaaa
    22321 ccacagccag aaatgtgata caccagcacg cggtcgccct ctttaaaatg gcggcagcct
    22381 tgccccatcg ccacaatctg cccgcacggt tcatgaccgt tgataaatcc ctggtataac
    22441 ggtttatcgg gtgccgccgc cgtggcacgg tgttgatgat agatatagtg gacatcgctt
    22501 ccgcaaatcc cggaggattt cattttgatc agtacctggt taatccccgg cgtcggcacc
    22561 gcaacttccc gcagatcgac ggttgaattt cctagtaaat aagcagccag catcgttttc
    22621 ataaatccct cattaacaat acgattaatt ttcatccctg cagcacccgc gccaggggcg
    22681 ttaaggttta gcgtttcgct ttactgcttc gctgggtcag caagatattc gccagcaccg
    22741 ccaccacgat gatgacgccg cgtaccacct gctggaaaaa ggagttaata ccgagcagca
    22801 ccagaccgtt accgattagc gtaatcacca gcacaccaag caatgtaccg aacagggaac
    22861 cgcgaccgcc ggaaagtgcc gtaccgccga ccacgaccgc ggcgatgacg tcaaactcca
    22921 gaccgtttgc ggcacctgcg ttaccggaac cgaggcgtgc cgccaacaaa atgccggtca
    22981 ccgccgctaa taatcccgaa agggtaaaga taagaatgcg cactcgacga acgttaatac
    23041 cgcacaactg cgccgccgtg gcattaccgc caacggcaaa aaccgagcgc ccgaaggcgg
    23101 tttttcggct aatgaacaca aacagcgcaa acaacaccat catgatcagc gcggatactg
    23161 gcacaccgag aaattgtccg cccagccagt ccagcacctc gttttcgtca atcggcactg
    23221 gcagtgcgtt ggtcataaac agccccattc cgcgcagagc gctccacagc cccagtgtgg
    23281 caacgaaact tggtacgtta aacacgccgc gcagcacccc ggcgagcgtt cccatcagcg
    23341 cgcctaacag caacaccagc agacaagcaa tcgccaacgg aacttcaaat tgcagcaaaa
    23401 atgccaggca caccgagaca aaagccacca tcggcccaac gctgacatca atttcaccgg
    23461 agataataat cagcgtcatc gcccaggcgg caatcccaat ggtggcggca tcgcgcagca
    23521 cgttcatctg gttattcaat gagataaagc caggcgcgtt cagggagaag accagataaa
    23581 gaatggcaat caccaccagc aaaccgatct cattaatatg gcgactgaca aattgtttga
    23641 gcgagacgct cttgccctgc ggcaatggta atgacgaagc atacatcttc aggttcctcg
    23701 gtaaaattca tcagtgcaca gacagaatgg cggacatcag ctcatccaca ttgaccggcg
    23761 agtgaaactc ctgcgagaac gtgccgtgct gtaataacag gatgcggtca cacaccagcg
    23821 gcaattcctc cacttcactg gagataaaca ccacgctttt tccttcggca gccagctcac
    23881 ggacaatacg gtaaatctgc tgtttggctt cgatatcgac gccgcgcgtt ggctcgtcga
    23941 gcaacaaaat ctggctggcg gcatagaccc aacgaccgat caccactttt tgctgattgc
    24001 caccagaaag cgtgccgatg ggtgtttcgc tactggcagc cttgaccgtc atccgctgca
    24061 tcacctcttc ggtcaggcgg cggatggtgg accattgcag cacaccgttg gtgctgattt
    24121 tttgccgatt ggtcagcact gtattttcgt caacgcctag ccagggaatg atccccgctt
    24181 ctttgcggtt ttctggcgta tagccaatgc cgcgtttcag catgtcgccg taatcggggc
    24241 gcatgatttt ctcgccgttg ataacaattt cgccctgttc atacgtctcc agcccaacaa
    24301 tcgccttcag caattcactg cgccctgccc ccagcaggcc agcaatgcca agcacttcgc
    24361 cacgacgtag cgtaaagctg atatcctcca gcttgggctt atggcgtaac gcacggactt
    24421 ccagtacggc ctgatccaca atttcctgag gggcaaccgg cgcaatatca acgtgatcac
    24481 gccctagcat tagcgacaca atatgatgcg tggaggtgtt ttcgagcatc acatcgcccg
    24541 ccacctgacc atcgcgcata acggtggcat aggaggcaat gcggcggatt tcttccatcc
    24601 ggtggctgac ataaatcacc gccacgccca gtgccgacat ctttttcacc gcactgatca
    24661 ccagttcaac ttctgcactg gcaagcgagc tggtgggttc atcaagaatg accacgcgcg
    24721 gctcgccctt catcacccgt gcaatttcca ccagttgctt ttgcgccggg cttagcgttg
    24781 aaacaagttg ttcaggacta acgtcaacgc ccagcgcctg taagcaacgt tgggcatcct
    24841 gcgccatttg caggtaatca atcatgccgt tgcggcgagg ccattgacca aggcagaggt
    24901 tttctgccac cgtcagccct tccaccagac ttaattcctg ataaaccgca cgaaccccca
    24961 gttcagcggc acggcgagtc aacgtagctt cgtcaccttc cagtcgcgtc tcgccaatcc
    25021 agatatcacc gctatccggg cgttcgctgc cggtaagcat tcgaatgaga gtcgatttgc
    25081 ccgcgccgtt tttacctaac agcgcacgaa cttcgccttt attgagcgtg aagttaacgt
    25141 tatccagcgc aacgacgccg gggtaacgct tatttcctgc caccactttt gctaccggga
    25201 ctgcctctgt tgccgtgaac atagggacct ctgcgaatca gcgattaggg cagaccatca
    25261 acgtgcgttg ccagccactg tttaccatct tccgttttgg tatagagatc gattggcacc
    25321 tgaatcactt tttcaccgtc ggcttgttta ttgataacct tcaatgtttg cgcgaaaaca
    25381 gcattgccca ttttcttacc ggaaatatcc actaccgctt tcagcacctg attgttttcc
    25441 agctcctgag caatttcggt tgtcatatcc gaaccgaaaa cagcaatttt tccggcctga
    25501 ttttgattac gtaccgcttt taccgcgccg agtgtcgcac cgcccgactc ccccataatg
    25561 gcgttgagat ccggcgtgga gataatcagt ttttcaccaa cggaaatcgc tttatctaaa
    25621 acagtccctt cctggttagc gacaatttgc gcgccgggaa cgcgggattt taatacttct
    25681 tcaaatcctt tacgtcgctg cacacaaact tcaaaggctt cgcaattgat gacggcaatt
    25741 ttcggctggt caattttatt ggcaataaaa taatcggcgg cagcgttacc cagtttttac
    25801 caaattccag tggatcgccg accagatacg ccgagacata tttatcgaca cccttttgat
    25861 taatacaggt gttgtagcaa atcaccggaa tacccgcttc actggcgcga cgaacggtac
    25921 ggctactgcc attttcagac actgccgata aaataatggc atcgacatta cgcgccacga
    25981 gggtatcaac aaaggtactt tctttcgaaa tatccccctg ggcgttagtt tcaattaact
    26041 gtacctgtac tgaagaatct tttgccgcat cctgaacgcc ctgacgcact ccagcgtaat
    26101 atccctgggt atcaaggtat attgcaccaa tggtcatttc tttatccgca gccctggcaa
    26161 atagtgcgct acctaatagc gtagccatta ataatagatt acgggttgtt ctcatttttt
    26221 taggcatagt gcctcctgta gggtttttat taacaacggc ttattctaat tattttgtga
    26281 tgagcggcag cgcgtgccgc tcaaatatta cagttaatag ggtttagtga acgtagttaa
    26341 aaataaaagg catcacagtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg
    26401 tcaattcagc gcgagcagac tcatattgcg caacctcccc gagtcccgcg gacgccagca
    26461 tcgccaccat caggcatttt tctttatgct gctgttgtaa atcgccatac agcgataaca
    26521 ggtcaggttg tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg
    26581 cccactgttt catttcacta aacagttgct gtgcggtttg ctgttcaccc agcagtcgca
    26641 gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt
    26701 taatggtgcg atcgccggtc gccgccagac gtaaacaacg catcgcttca gtttcatcgc
    26761 cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta
    26821 aacggccttc gcttaaattc tccggataat acagcgcggc atgaagcagt tcgctggcct
    26881 gctgcggctc tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact
    26941 gactggtgac cttcccttcc ccgccttccc acgggtggaa tttacgcgtg gcgagaatgt
    27001 ccgccgcttt gtctgcctga cccgtgagat gccacaaatt gagcagttct gcggtcatgt
    27061 cgtcgcgttt cagcgcaatt tccagattat tttccagacg cgccagtcgt ttctccggtg
    27121 tggctccact taacttatcc agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg
    27181 caagctgata agcattatca agataacgcg cggccagctc ataatcgtgt ttcttattcc
    27241 acgcatggat cgctaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac
    27301 gttgccataa ggcaatggct ttgttgtagc tacgtttgtt gtagtagaag caggccagta
    27361 aatggcgagc aaaccaacac tcttcaatac tctccagcgc cgccacttct tccagcgtat
    27421 tcgggaaacg gacaaactgc gggaagacat caatggcttt tgcgaccagt tcgccacgtt
    27481 cggctttcgg cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg cagtccagtg
    27541 cgttcagcat ctctgccgcc agggtgggca ttccccagtt aatcagttgc ccggcggtca
    27601 tcagagcgtt aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg gctgattcgc
    27661 tacgaccatc gaaccagttc agccaccaca gagtggcgtt cagcggataa tcacgcagca
    27721 gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact taacaccagc agcagattat
    27781 gcaagcaaag cacttcctga ttggttgggc aggcacgaag actttgttgg caaaaatcca
    27841 gacccgcgtc gaagttatca ttacgcgccg ccagtcgtgc cagaccataa tagccgcctg
    27901 ctttactatt gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgtc
    27961 cctgacgttc gtaagcactg gcgcgaatca aacttgcttg tccacactgc ggttttttgt
    28021 tcagcgcatg tgcacgtttc agcgcctgac tggcatacgc caccgcttgc gggaaatctg
    28081 cgcggttata ttccagcatc gccagcgcca ggttacagcg ataatccagc gggtccagcg
    28141 ccacgccgcg caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat
    28201 gctgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt
    28261 tggcgacgtc cggcaacggc aactcctgcg gctgatgttc ctgataactc agtacaatat
    28321 tgccgtcggc atcggagagt tcaatggtca gtctgtcagg attgataccg tgcaacacgc
    28381 cctggatggc ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg ttgcatttgc
    28441 cgatttcgcg gatcgccaga cggtatccgt tcaacggaga gatggcatac agcccccact
    28501 caatcccccg ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca
    28561 tgcccagaga atgataaggc aggaaatact gctcgaaacg cttctcttcg taagcatcaa
    28621 gccaggtaaa atcaggctgg ttatcggcaa aaataccggt catcagttcg atatacgggc
    28681 cgttattgtc ggtcagactc ttatcccacg cctggccaaa ttcactgtgt ccccaactcc
    28741 actgtttttt acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat
    28801 cgtgacacca cgcgccgaca aaatcgtact gcgatttttc ggccatatat gaggttggaa
    28861 caggcacatt tttatagcga gaaatgtcca ctccggcgga gtagtccact ttgtagtaag
    28921 tgccggtggc gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac accgcagtca
    28981 catccggtgg gaaaacgctc tgatgccctt cacccccttt cactgccggg ttgacccacc
    29041 acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt tccagtgccg
    29101 cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt
    29161 cgccgaccca caccgtctgt gcaccgtctt catgggcttc gagggtgaaa tcaacgggca
    29221 taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccgccg gagatccacg
    29281 gtcccagcat ccccaccagc gcaggtttaa tgacttcatt gtgatagaca aaatcgcgct
    29341 gtttcacttt atcccatgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt
    29401 tgatgtagtc gttttccagc cacaccgcct gctaggattt cagggttttc tgctcgctca
    29461 gcgtatcggt cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt
    29521 ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct
    29581 cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa
    29641 aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt
    29701 aactaaacaa ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca
    29761 taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct
    29821 ggcccggctg gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag
    29881 cgaaaaacgg gtggtgaata ttgacgatga aagccagacg cgcgggcata gtagcggtac
    29941 atggctgatt gcgccggaag gtgactggac gctgtgcctg aacgtgacgc ccaccagtat
    30001 tgagtgtcag gttgctaatg cttgtttaag tccgaaaggc gaatttgagt atttacagat
    30061 tgatgtaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg
    30121 taaattgtgg ccggaccgca ccatcaatct ggcgctggca atccacgggc aggttgatcc
    30181 ggtgaccggc gtatcgcaaa ccatgccgca agcgccgtgg acaacgccag ttgaggtgaa
    30241 gtatctgctg gaagagaagc tgggcattcg ggtgctggtc gataatgact gcgtgatgct
    30301 ggcgctggcg gagaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt
    30361 tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta
    30421 tggtagcgga cagattggtc acaccatcgt taatccggat ggagtcgtct gcgactgtgg
    30481 acgttacggc agcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac aggcgcgggt
    30541 atggctaaaa tcacaaccgg ttaatactca gcttgagcct gaaaaactga caaccgcgca
    30601 gttaatcgct gcctggcaga gtggagaacc gtggatcacc agctgggttg atcgctctgc
    30661 caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg
    30721 gttgtacggt cgcagttgtg cctttggtga gaactggctt aatactatta ttcgccagac
    30781 aggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc aaattggctt
    30841 cgggcaatta agccgcgcac aacaggtgct gggaattggc tatttgtatg ttgaggcgca
    30901 gttacgacag atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttgatga
    30961 tcataaacga aaaattgcct gatgcgctac gcttatcagg cctacacgga gattgcaata
    31021 tattgaattt gcaagatttt gtaggcctga gaaggtgttc atgccgcatc caacatgaac
    31081 aacgagcaca ttgtcagcaa atcaccgttt cgcttatgcg taaaccgggt aacgtgcgca
    31141 gatgtcgaga actttacctt tgatgcgctc gataacggct tcatcattga tgctgtccag
    31201 cacgtcacac atccagccag ccagttcttt cgcttcggct tctttaaagc cgcgacgggt
    31261 aatcgccgga gtacctacac gaataccgga ggtcacaaac gggctcttcg gatcgttcgg
    31321 tacgctgttt ttgttgacgg tgatgttagc acggcccaga gcggcgtctg cttctttacc
    31381 ggtcaggttt ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc cgccggaaac
    31441 cactttgtag ccgcgctcga ggaacacttc taccatcgct ttagcgtttt tcgcgacctg
    31501 ctgctggtaa gttttgaact caggctccat cgcttctttc agagcaaccg ctttaccggc
    31561 gattacgtgc atcaacggac cgccctgacc accagggaaa acggcagagt tcagtttttt
    31621 gtacagctct tcgctaccac ctttcgccaa gatcaggccg ccgcgcggac ccgccagggt
    31681 tttgtgagtg gtggtagtaa caacgtgagc atgaggaacc gggttcgggt agacgccagc
    31741 agcaaccagg cccgcaacgt gcgccatatc aacgaacaga taagcaccga tgctgtcagc
    31801 gatttcacgc attttcgccc agtcaaccac gccggaatat gcagagaaac caccgataat
    31861 cattttcggc ttgtgttctt tggcttgttt ttccagatcg gcgtaatcga tatgaccggt
    31921 agcatcgata ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa ccggagaacc
    31981 gtgagtcagg tgaccgccat gcgccaggtt catacccaga acggtatcac ctggttccag
    32041 cagcgcggtg tagaccgcaa agttagcctg ggagccggag tgcggctgga cgttagcgta
    32101 gtcagcgccg aacagttctt tcgcacgatc gatcgccagt tgttcaacga tatcaacata
    32161 ctcgcaaccg ccgtagtagc gtttgcccgg ataaccttca gcatatttgt tggtcagctg
    32221 agaaccctgc gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat
    32281 gtgctcttcc tgacgtactt tttcctgctc catagcctgc cacagttcgg catcataatc
    32341 ggcaatgttc atttcacgct ttaacatccg catctcctga ctcagctaac aataaaattt
    32401 ttggccttta taggcggtcc tgttggacaa cggcgaacag tataaccgaa tcattgtgcg
    32461 ataacaggtc ttgacaaagg aatttacgca aacgattacc ttcaggctac gcaaggcttt
    32521 ggagaataaa gagcttgcaa ccggaaacgg atttcttttc aggtttgtga tgcaaatttt
    32581 tcacttcatc acattctttc tgaaaaacac caaagaacca tttacattgc agggctattt
    32641 tttataagat gcatttgaga tacatcaatt aagatgcaaa aaaggaagac catatgcttg
    32701 acgctcaaac catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa
    32761 agttaaccgc ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt
    32821 ttaacatgag taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg
    32881 cctacgccag taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc
    32941 agaagcacac cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt
    33001 tggcaacgct ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag
    33061 cctatggtgt actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg
    33121 ccagcaaagc cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc
    33181 gcagcgcgct tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat
    33241 accgtccggg gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa
    33301 ttcgtcagta ctctttgact cgcaaaccgg atggcaaagg ctatcgtatc gcggtgaaac
    33361 gcgaagaggg tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg
    33421 tgaaactggt cgctccggca ggtgatttct ttatggctgt cgcagatgac acaccagtga
    33481 cgttaatctc taccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa
    33541 aagcaggcca cacagcacaa gtgaactggt tccatgcggc agaaaatggc gaagttcacg
    33601 cctttgctga tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct
    33661 ggtatcgtca gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga
    33721 tggatttgag caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctctgcg
    33781 gcccggttgg cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg
    33841 aaaacattca ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat
    33901 ggaaacatcc ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac
    33961 ggatgcgaat gacccgagcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg
    34021 tttgcgccgt gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa
    34081 tctcaatttt cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg
    34141 tatggccttt ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga
    34201 cttcggccag tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct
    34261 ttttcatgct attccttgaa aaggttgcct gtcttttgat ctgctaaacg taacacataa
    34321 cgccaattca ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa
    34381 attctgtccg gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtgattt
    34441 gcagcagctt acgcaagtag ttgagttcaa aatgattacg tgcctcaaca aaggttggca
    34501 gcgccgtatt ttcaccctcc agcgcctgct ccaccagcgc atcactaatc accggagatg
    34561 aggtcagcgc cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc
    34621 tcgcggtcat caggcgtttc atcgcatcgg tagagaacgc gcggacaaac ggtttatgtc
    34681 gctctgccgc ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct gtgcgctccg
    34741 ccagtgccgg aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt
    34801 ccccgcgcgc catcgctttt gacagatcac ggtgagtggc agaaataatc cgcacattga
    34861 tatcaatatc gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca
    34921 gtttgacctg taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt
    34981 ccgccgcctg gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat
    35041 gaccaaacag ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa
    35101 atggtttgct gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg
    35161 tgccgctctg accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt
    35221 ccagcaaacg cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg
    35281 tggctggcgc ggattgctcc agcgcatcgt caattgcctg atatagcgca tctttatcga
    35341 caggcttggt gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag
    35401 aaccatgcgc agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa
    35461 atagctgcat accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt
    35521 cgcgattcag tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt
    35581 cgctggtcag gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata
    35641 aatgcgcagg tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca
    35701 ctctcgtgcg gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga gagctggcgt
    35761 tcaatatcgg tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg
    35821 tgatgttgct ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa
    35881 cgttggcgct cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac
    35941 agtgggcgaa cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg
    36001 cggcgctcat acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt
    36061 tgccagctgc cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga
    36121 tcagcacaat ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc
    36181 agcgcccaga tgtcgctaca ttcagtagaa agatagtcag ccagttgata aaccggaatt
    36241 ttttcttctg ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat
    36301 gccagacatg gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt
    36361 cgcatattca ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga
    36421 catggacagc gcggagaaac cggggcgtac atatcgtacg tgaagatttc gagcactacc
    36481 cggggccgaa ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt
    36541 ttttcgacga cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat
    36601 acagttcccc ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc
    36661 ccttcaccgc cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc
    36721 gttcctcttg cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat
    36781 gtaaactgct gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt
    36841 aaagattatc cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga
    36901 gatcgacgtc ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg
    36961 tctccaccag cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac
    37021 ggttgtaatc aagcagttgt tcgatcagtt tttgcaaatt gcagctgctg ctatcaagaa
    37081 tgctcaccac ctctttttgc tctggcgtaa gcggcccgac aacctgatca gccagtaatt
    37141 cagtgccctc gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa
    37201 attgatggcg ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc
    37261 caaccgagcg taactcgctc ggtccacaga acgaaacgct attgcccaga gaacgccctt
    37321 cccccagacg gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga
    37381 aaagtagcac catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt
    37441 gcccacgttc ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg
    37501 tggcctgcac catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag
    37561 catcgggacc actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg
    37621 cctggtaaag tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac
    37681 gcttgcgctg gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac
    37741 ggtagctacg ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa
    37801 taagcgtagt gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg
    37861 cttgccatgc cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt
    37921 gtcgtaatga gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga
    37981 atagcctgat gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt
    38041 tgttatggaa taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc
    38101 tgatatcaga agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac
    38161 gtcccgaagg ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact
    38221 gtgccaataa agaaaatagt ttggtaacgt actgattata cgttgctttg agggatttat
    38281 ggcttcttcg ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acaacttaag
    38341 ataaacttat tagattgcaa taaaatcaat catttaagtg tcgccagaaa gcgacacggt
    38401 aaaccaccca ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga
    38461 tagtggttgg tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgacttaccc
    38521 caactgctta cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc
    38581 cggatgccag gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt
    38641 gactcgacca ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt tcgggttagc
    38701 cgggtaggtt tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt
    38761 gctttccagt gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga
    38821 gacagcaatc ggcatttgcg agcccaccat cccctgcaac agcagagacg ggctttgggt
    38881 tacttcaacc aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg
    38941 ccacaactca ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca
    39001 tacccccagg gccagcgttt gcggacgatg gaagaaggtt gcaaactcat cgcgtacacg
    39061 gtcattgaac aggatagact tcgcccaacc ttcaccggca cccagcacat caccgtagga
    39121 gaaacctccg cacgcgacca gggcgtgaaa atcctccagt cccgtacgtc cagtcagcag
    39181 gtcgctcata tgcacgtcga tagcatcaaa gcccccacgt tggaaagctg ccgccatttc
    39241 aacatgcgag ttcacgccct gctcacgcag tacggcaact ttcggacgtg cgccagtggc
    39301 aatatacggt gctgccacat cttcgttgat atcgaacgac agttttacat tcaggcccgg
    39361 atcggcgtcg ttagatttcg cctgatgctc ctgatcggca cactccgggt tgtcacgcag
    39421 gcgctgcatc tgccaggtag tttctgccca ccagacacgc aacgtagtgc ggctttcgct
    39481 gaatacagtc tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgcccaac
    39541 ataatggacg caatccgcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc
    39601 agcggcacga acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc
    39661 gtcacccaga gaggcgatat ccgcgtcaat gccacaatga cctgcaaagg ccatttccgc
    39721 cagcgttacc agcaggcctc catcagagcg gtcgtgatac gccagcagct tacgctgtgc
    39781 aaccagcgcc tgaatcgcgt catagaagcc tttcagttgg acaacatcgc gtacatctgc
    39841 cggtttgtcg ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt
    39901 gcctttgccc aaatcaatca gcagcagtgc gttatcttcg gtacaaagct gcggcgtgat
    39961 ggtgtgacgt acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt
    40021 catttcgcgc tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc
    40081 caccgggatc gtcaggccca gcgccggaca aagttcttca cccacggctt taacggcttc
    40141 atacaggccc gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt
    40201 aatgcgtttg atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc
    40261 cagacgggca gaggcggcga aatccagcag cgcaaccggc gcacgctcgc caatcgccat
    40321 cgcttcaccg tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac
    40381 ctgccacggc cccaccatct gatcgcgcgc caccatgccg gttacgctgc ggtcaccgat
    40441 ggtcaccagg aaagttttct ccgccacggt cggcagatgc agtacgcgtt tcaccgcgtc
    40501 tgcaatggtg atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc
    40561 gcgggtcatc ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt
    40621 atcaaaatga cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc
    40681 gtagggtgcg cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac
    40741 cgccagcacg tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg
    40801 ttcgtcgctt agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt cgctcaccag
    40861 ctccggcatc gcgttagaaa gaccgcccgc accgacgtcg tggataaaca gaattgggtt
    40921 agcatcacca agctgccagc aacggtcgat cacttcctgg caacgacgct ccatctccgg
    40981 gttgtcgcgc tgtacggaag caaagtcgag gtcggcatca gactgaccag acgccatgga
    41041 agacgccgcg ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt
    41101 cgcaccgacg ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc
    41161 cgccagcatg atcggtttgt gataaccgcg cagctcttcg ccattgtggc tgttcacttt
    41221 ttcttcataa gtacggaagt agccgttcag tgccggacga ccaaattcgt tgttaaacgc
    41281 cgcaccgccc agcgggcctt cggtcatgat gtccagcgcg gtaacaatgc gctcaggctt
    41341 accgaaatct tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg atacggagaa
    41401 accaaccaaa ccagctttcg gcttcgcgcc gcgcccggtg gcaccttcat cgcggatttc
    41461 accgccggaa ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt
    41521 ttcgactttc atcaggatgt gcgccggttc ctgatggaaa ttgtagcggc ccgtttcgtg
    41581 gtcagcaaag tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc
    41641 agagagaacg tgatctggcg tggtttcgaa agtatttttg atcatcctga acagcgattt
    41701 cggctgttgt tcaccgtcga taatccagtc ggcgttaaaa attttgtggc ggcagtgctc
    41761 agagttcgcc tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt
    41821 gaaagcatcc tgcagatagt caatttcatc ttccgccaga gccaagccaa gacgcaggtt
    41881 agcgtcgatc agcgcctgac ggccctgccc cagcaaatca acgcttgtga ccggagtcgg
    41941 ttgatggtgg gcaaacaact gctctgcatc atctaaatca aaaaagaccg tttccatcat
    42001 gcggtcgtgc agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc
    42061 tatatagtaa gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc
    42121 aatatcggtc gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag
    42181 gagtttgcct tgcggggcgt ggctggcgag tgccggacca tatttcagca ggcgttcaag
    42241 ttgtgcgtgc tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc
    42301 gtaaatattg tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg
    42361 gaatgccgac agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa
    42421 gctttcggtg tacccaaggg gggggaaacg ggcgtcatta taaagaatct gatgcgctga
    42481 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    42541 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    42601 cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa
    42661 ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc
    42721 gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa
    42781 gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac
    42841 gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggcgta
    42901 aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct tgataatggt
    42961 aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag
    43021 cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg tcagtatcgc
    43081 ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg tcatgtggta
    43141 gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg gaaggtagac
    43201 gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc
    43261 attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg
    43321 ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc
    43381 ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac gctggcacgc
    43441 attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt
    43501 ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa
    43561 gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag
    43621 gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc
    43681 ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg
    43741 caggatatga tgagtaaagt gccggaaagt gtgccggaga acgaacggat ctggtttgcc
    43801 ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa
    43861 accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag
    43921 aaaccctatt acagcaagct gacttacggc tacgctcgtg ggcatgaagc ctacgcttat
    43981 gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag
    44041 caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag
    44101 ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg
    44161 acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc
    44221 gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca
    44281 ctgagcaacg ccgcgcactc atccgccagt attccttccg taatttccac tcggtgattc
    44341 atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc
    44401 gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat
    44461 ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca
    44521 ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg
    44581 ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc
    44641 cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccaata ttcgtggcta
    44701 aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga
    44761 cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga
    44821 tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc
    44881 cgcagcggcg tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc
    44941 atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc
    45001 ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc
    45061 aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga
    45121 aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt
    45181 gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca
    45241 cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca
    45301 ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc
    45361 aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc
    45421 tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca
    45481 cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta
    45541 gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat
    45601 ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaagggctt
    45661 gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat
    45721 ttaagctctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc
    45781 gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg
    45841 gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg
    45901 ttacggctgg tcggcgaaaa actgattaaa gaaaataccg ccgctatgta cgcaacgcta
    45961 aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg
    46021 ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg
    46081 atgaagattg gcttcaatgc tgccgcactg cgcgatatgc atgcgctact cgcaacagta
    46141 caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag
    46201 ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc
    46261 tttactccga atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa
    46321 caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg
    46381 ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagt
    46441 gaagcgctgg tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt
    46501 gtgttgtttt gagagtttcc ttatggcgct gttaatcact aaaaaatgca tcaattgtga
    46561 tatgtgtgaa atcaaataaa aacacattta ttttcatagt gatacagcaa tgtgttattg
    46621 aatgtgttgt tggatttatg gtgcaaaaaa attttgcata agttcagcaa cacaaagaac
    46681 accactttct ttaacgtcct ccgcgcccta aaggacgggg ctttacggcg caaaaggcct
    46741 gtgacataca acattaactt ttcagtcaac cgtagcacgt tcttgtatac gccgtgtctg
    46801 cggttttata taaccctggc tggaaacctc ttatacaaag tcgattcgcc aacatcatag
    46861 atgatcgcca ccttctggcg agaaactcct gatgcaatta gtcgcccggc ctgcgcccat
    46921 tattctggtg aaagtttggg acggcgaccg ccaattcgtc cctgtgcgcg agcagcttcc
    46981 agtcctgctt ttgttcgttc aacaatcagt tcacgttcca tttcagccag ggcacccatc
    47041 acatggaaga aaaagcgtcc cattggtgtg ctggtatcaa ttgaatcagt cagactacga
    47101 aagttgatgc ctcgttcgcg caacgcctcc accagcacga caagatgccg catactgcgc
    47161 cccagccgat ccagcttcca gacaaccagt gtgtcgcctg ccgataatgt cttgagcagt
    47221 tttttcagcc ccggtctttc ggactttgta ccacttatct tgtcttcaaa aatcagctcg
    47281 catcct
//
LOCUS       DAJTUP010000021        47191 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTUP010000021 DAJTUP010000000
VERSION     DAJTUP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47191
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.021"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(259..876)
                     /gene="tdk"
                     /locus_tag="OO468_001216"
     CDS             complement(259..876)
                     /gene="tdk"
                     /locus_tag="OO468_001216"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR6258404.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1481..1894
                     /gene="hns"
                     /locus_tag="OO468_001217"
     CDS             1481..1894
                     /gene="hns"
                     /locus_tag="OO468_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR6258405.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2038..2946)
                     /gene="galU"
                     /locus_tag="OO468_001218"
     CDS             complement(2038..2946)
                     /gene="galU"
                     /locus_tag="OO468_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR6258406.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3148..4161)
                     /gene="rssB"
                     /locus_tag="OO468_001219"
     CDS             complement(3148..4161)
                     /gene="rssB"
                     /locus_tag="OO468_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR6258407.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4253..5158)
                     /gene="rssA"
                     /locus_tag="OO468_001220"
     CDS             complement(4253..5158)
                     /gene="rssA"
                     /locus_tag="OO468_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR6258408.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5271..5729
                     /locus_tag="OO468_001221"
     CDS             5271..5729
                     /locus_tag="OO468_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR6258409.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5779..6621
                     /gene="purU"
                     /locus_tag="OO468_001222"
     CDS             5779..6621
                     /gene="purU"
                     /locus_tag="OO468_001222"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR6258410.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6781..6865
                     /locus_tag="OO468_001223"
     tRNA            6781..6865
                     /locus_tag="OO468_001223"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6815..6817,aa:Tyr,seq:gta)
     gene            6912..7043
                     /locus_tag="OO468_001224"
     ncRNA           6912..7043
                     /ncRNA_class="other"
                     /locus_tag="OO468_001224"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7227..7904)
                     /gene="narI"
                     /locus_tag="OO468_001225"
     CDS             complement(7227..7904)
                     /gene="narI"
                     /locus_tag="OO468_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR6258411.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7904..8614)
                     /gene="narJ"
                     /locus_tag="OO468_001226"
     CDS             complement(7904..8614)
                     /gene="narJ"
                     /locus_tag="OO468_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR6258412.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8611..10149)
                     /gene="narH"
                     /locus_tag="OO468_001227"
     CDS             complement(8611..10149)
                     /gene="narH"
                     /locus_tag="OO468_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6258413.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10146..13889)
                     /locus_tag="OO468_001228"
     CDS             complement(10146..13889)
                     /locus_tag="OO468_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR6258414.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14282..15673)
                     /gene="narK"
                     /locus_tag="OO468_001229"
     CDS             complement(14282..15673)
                     /gene="narK"
                     /locus_tag="OO468_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR6258415.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16012..17808
                     /gene="narX"
                     /locus_tag="OO468_001230"
     CDS             16012..17808
                     /gene="narX"
                     /locus_tag="OO468_001230"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR6258416.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17801..18451
                     /gene="narL"
                     /locus_tag="OO468_001231"
     CDS             17801..18451
                     /gene="narL"
                     /locus_tag="OO468_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR6258417.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18452..19846)
                     /gene="ychO"
                     /locus_tag="OO468_001232"
     CDS             complement(18452..19846)
                     /gene="ychO"
                     /locus_tag="OO468_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR6258418.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20031..20384
                     /gene="ychN"
                     /locus_tag="OO468_001233"
     CDS             20031..20384
                     /gene="ychN"
                     /locus_tag="OO468_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR6258419.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20428..21123)
                     /gene="chaC"
                     /locus_tag="OO468_001234"
     CDS             complement(20428..21123)
                     /gene="chaC"
                     /locus_tag="OO468_001234"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR6258420.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21281..21511)
                     /gene="chaB"
                     /locus_tag="OO468_001235"
     CDS             complement(21281..21511)
                     /gene="chaB"
                     /locus_tag="OO468_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR6258421.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21781..22881
                     /gene="chaA"
                     /locus_tag="OO468_001236"
     CDS             21781..22881
                     /gene="chaA"
                     /locus_tag="OO468_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR6258422.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23286..23393
                     /locus_tag="OO468_001237"
     CDS             23286..23393
                     /locus_tag="OO468_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR6258423.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23542..24396)
                     /gene="kdsA"
                     /locus_tag="OO468_001238"
     CDS             complement(23542..24396)
                     /gene="kdsA"
                     /locus_tag="OO468_001238"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR6258424.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24432..25241)
                     /locus_tag="OO468_001239"
     CDS             complement(24432..25241)
                     /locus_tag="OO468_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR6258425.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25245..25637)
                     /locus_tag="OO468_001240"
     CDS             complement(25245..25637)
                     /locus_tag="OO468_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR6258426.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25634..26467)
                     /gene="prmC"
                     /locus_tag="OO468_001241"
     CDS             complement(25634..26467)
                     /gene="prmC"
                     /locus_tag="OO468_001241"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR6258427.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26467..27549)
                     /gene="prfA"
                     /locus_tag="OO468_001242"
     CDS             complement(26467..27549)
                     /gene="prfA"
                     /locus_tag="OO468_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR6258428.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27591..28847)
                     /gene="hemA"
                     /locus_tag="OO468_001243"
     CDS             complement(27591..28847)
                     /gene="hemA"
                     /locus_tag="OO468_001243"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR6258429.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29061..29684
                     /gene="lolB"
                     /locus_tag="OO468_001244"
     CDS             29061..29684
                     /gene="lolB"
                     /locus_tag="OO468_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR6258430.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29684..30535
                     /gene="ispE"
                     /locus_tag="OO468_001245"
     CDS             29684..30535
                     /gene="ispE"
                     /locus_tag="OO468_001245"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR6258431.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30686..31633
                     /gene="prs"
                     /locus_tag="OO468_001246"
     CDS             30686..31633
                     /gene="prs"
                     /locus_tag="OO468_001246"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR6258432.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31758..33437
                     /gene="dauA"
                     /locus_tag="OO468_001247"
     CDS             31758..33437
                     /gene="dauA"
                     /locus_tag="OO468_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR6258433.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33492..33770)
                     /gene="ychH"
                     /locus_tag="OO468_001248"
     CDS             complement(33492..33770)
                     /gene="ychH"
                     /locus_tag="OO468_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR6258434.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34048..34632
                     /gene="pth"
                     /locus_tag="OO468_001249"
     CDS             34048..34632
                     /gene="pth"
                     /locus_tag="OO468_001249"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR6258435.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34749..35840
                     /gene="ychF"
                     /locus_tag="OO468_001250"
     CDS             34749..35840
                     /gene="ychF"
                     /locus_tag="OO468_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR6258436.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36684..39551
                     /locus_tag="OO468_001251"
     CDS             36684..39551
                     /locus_tag="OO468_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6258437.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLSAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39651..41570)
                     /gene="dhaR"
                     /locus_tag="OO468_001252"
     CDS             complement(39651..41570)
                     /gene="dhaR"
                     /locus_tag="OO468_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR6258438.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41798..42868
                     /gene="dhaK"
                     /locus_tag="OO468_001253"
     CDS             41798..42868
                     /gene="dhaK"
                     /locus_tag="OO468_001253"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR6258439.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDAMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42879..43511
                     /gene="dhaL"
                     /locus_tag="OO468_001254"
     CDS             42879..43511
                     /gene="dhaL"
                     /locus_tag="OO468_001254"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR6258440.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43522..44940
                     /gene="dhaM"
                     /locus_tag="OO468_001255"
     CDS             43522..44940
                     /gene="dhaM"
                     /locus_tag="OO468_001255"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR6258441.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45260..46957
                     /gene="treA"
                     /locus_tag="OO468_001256"
     CDS             45260..46957
                     /gene="treA"
                     /locus_tag="OO468_001256"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6258442.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
BASE COUNT        11822 a        12172 c        11581 g        11616 t
ORIGIN      
        1 gtttttttgt ttacccaaag caacttttcc ataaaccgac agcattagcc ttcatcatat
       61 ttgcgacgat gtataacgcc taaacacagg gatattgtac tttacaggtc acaagtcaac
      121 gtcggtgctt aagagccctg tgaggcgtat agcggcgtta aaaaactgct gagaagggta
      181 tatagcccgg aagaagtgcg taaaacgaac tgacaggata aaagtgccct gctcaccctg
      241 tcagtaaaga aattcttatt aatcgtggcg atgcctttcc tgaatagccg ttaatgagcc
      301 gacttgtaac gcctctttat agtgtttacg gcatacagaa acgtatcgtt cattaccacc
      361 aattaccacc tgctcacctt cgttataagg tctgcctgct tgatcaagac gcagcaccat
      421 gcttgcttta cggccacaaa aacagatggt ttttaattca accagtttgt cggaccatgc
      481 cagtaagtat tggctgccaa taaataattc acctcgaaaa tcggtacgta aaccataaca
      541 aagtacgggt atatcgagtt gatcgacaac ctccgataat tcatatactt gttgtctggt
      601 taaaaactgg cattcatcaa ccagtacgca atgaattgcc tgctgttcat gttccgcacg
      661 aatctcatca aataatgatg aattttggtt aaataatttt gcaggcgatg acaaacctat
      721 acgcgaactg actttcccgg caccaaagcg atcatcaatt tctgccgtat atacgacagt
      781 gcgcatgccg cgttcctggt aattgtatga agattgcaac aatgctgtag acttacccgc
      841 attcattgcg gaatagtaga aatatagctg tgccatcagc cacaggccct caatgatatg
      901 aaataaagtt ggctggagtt tatcataatt cgtcagtttt tcagctcaac agtatgcaca
      961 gaagtgggta tatgcgttct cccttacgaa gccttgcata atccttctga gctatcatta
     1021 caactgcctc gcttgttata agcgggtaaa tgactgctgg taactattca caatctttaa
     1081 cctgttgcgc aagtaatagc cctctgttga cctccaggag atagtgcaat actaagtcca
     1141 tgctcttatt gcgactgttc tacttttcat cattcgctta atagggaatt ctcgtaaaca
     1201 caactaatac agaagactga aaggtcgtca gcctacgata atctccccat aaaatgtgac
     1261 atgaatcagg aagttttaac ctcacgtgct gcgaaatcat cggtgtaaat agggctatat
     1321 gccgcgtctt ttctggctaa ttttatgaaa agatatttat tggcggcaca aaataaagaa
     1381 caattttgaa ttccttacat tcctggctat tgcacaactg aatttaaggc tctattatta
     1441 cctcaacaaa ccaccccaat ataagtttga gattactaca atgagcgaag cacttaaaat
     1501 tctgaacaac atccgtactc ttcgtgcgca ggcaagagaa tgtacacttg aaacgctgga
     1561 agaaatgctg gaaaaattag aagttgttgt taacgaacgt cgcgaagaag aaagcgcggc
     1621 tgctgctgaa gttgaagagc gcactcgtaa gctgcagcaa tatcgcgaaa tgctgatcgc
     1681 tgacggtatt gacccgaacg aactgctgaa tagccttgct gccgttaaat ctggcaccaa
     1741 agctaaacgt gctcagcgtc cggcaaaata tagctacgtt gacgaaaacg gcgaaactaa
     1801 aacctggact ggccaaggcc gtactccagc tgtaatcaaa aaagcaatgg atgagcaagg
     1861 taaatccctc gacgatttcc tgatcaagca ataatctttt gtagattgca cttgcttaaa
     1921 atcccgccag cggcgggatt ttttattgtc cggtttaaga caatttaata agaataagct
     1981 ataaaaaaac ggcgtcgatt gctcaacgcc gtttcgtgga taacaccgat acggatgtta
     2041 cttcttaatg cccatctctt cttcaagcca ggctttaaat tccgtgccaa gggtgttatg
     2101 acgaataccg tattcaacga aggcctgcat gtaacctaat ttattaccgc agtcatggct
     2161 cttccctttc atatgatagg cttccaccgt ttctttttcg atcagcatat caattgcgtc
     2221 ggtgagctga atttcatcac cagctcccgg aggggttttt gccagcaacg gccaaatatc
     2281 cgcgctaagt acgtaacgac ccacaatagc gagattagac ggcgcaacat ccgctttcgg
     2341 tttttctacc acaccaacca tcggtacgct ttcacccggc gctaattcaa cgcctttgca
     2401 atccacaacg ccatatgcgg tcacatcagc aaccggttca accatgatct ggctatgacc
     2461 cgtttcatca aagcggcgga tcatctctgc caggttatcc tgtgacaaat cggattcata
     2521 ttcatccaga ataacatcag gcaaaataac agctaccggt tcatcaccca ctaccgggtg
     2581 agcacacaat accgcgtgtc ccaggccttt cgccagaccc tgacgaactt gcataatagt
     2641 cacgtgcggt ggacaaatag actgcacttc atcaagcagt tgacgtttta cacgtttttc
     2701 cagcattgct tccagttcaa aactggtatc aaagtggttt tcaatagagt ttttagatga
     2761 gtgtgtaacc agcacaattt cagtaatgcc agccgcaata cattcattca cgacgtattg
     2821 aattaatggc ttatcgacaa gtggcagcat ctctttcggg atggctttcg tcgccggcaa
     2881 catcctggtt cctaatcccg caacggggat aacggctttt ttgactttcg tattaatggc
     2941 agccatttaa attctcctgg actgttcgtg ttttgaacgt gttcatattt ctgtatcgca
     3001 tcccagtata tcagcacccc gaaggggcta agttctgaaa aggaggcgag atagcataat
     3061 taagacaatt acttatacga atggcgacga tagtaccacc agccgacatt agcaggtaat
     3121 gcaaatttag cccgcgttat cgtttgctca ttctgcagac aacatcaagc gcagtcgacc
     3181 accggttccc catatttggc attgccaggc atcgcatcgc tggctcaatt gattcaaata
     3241 agcgttacct aaagtgccta acggaacacc attactgatt tgcacctggt gttcgccggt
     3301 atttaacgtc gcattcagac ccgcagaaac cagaatgaga tttttcagtt cgcgatgata
     3361 atagccaact aatagcggaa actgccccgg cagattggcc tgacgaagta aatggtttac
     3421 ctgcttcaat aacgcgccca actctggcaa ccgttgattt tggtgtgcaa gctgttcctg
     3481 taataatccg ttaaacaatg cgcgtaataa caaggcagca agtacgccat tatgtccagc
     3541 tcgggtgaca tcaaggcaat aaaatgccag atcgttttcc gaaagtgcgg caatatcaag
     3601 caccaggccg ggtttgtccg cggcaaccaa ttgacggtaa ttaacccggc aatgggaaat
     3661 cacctgctga accggcggtt gtagttcctg taataatttc gccgctgcgg cagggttatc
     3721 aaccattgca tcccagtcgc gaaaaagcct ttcctcttcc tcaacgcgcg aattaaacat
     3781 gctgggatag agacaggcaa aaaccatctc gcgcaagcga ttcagatctt taactggttt
     3841 cagcaaaaca tcttcaacgc ccagacgtaa cgctttggca atatctgcca tattttcagt
     3901 ggcagatatc accagaactg gggtctggtc gcctctgtta cgtatatgct ccagcagttt
     3961 aagcccgttc attcgtggca tcgcgatatc acatatcatc aggtctggag tgaaacctcc
     4021 cagcaactca agggcatcca ccccatcagc cgccagtacc gttgtcgctc ccaatgagga
     4081 aaaccatgaa tccagaagcg agcgaaatac ctgctcatct tcaacaatga gaatctgttt
     4141 tccgaccaat ggctgcgtca tgttctctcc cctgactggc tttactcaat agtggcatgc
     4201 tattgccacc tgcgcctgtc agaattaact taagtgtaga taaaaattct ggtcaaatgt
     4261 tggtgcgtac caacggcaaa agttcgtcca ttttcctttc cactgccagc tgtccggccg
     4321 caatggcagc gtgcgcgcga tggaaatcaa gcgtagatat ttgcgggcaa acaggttgaa
     4381 tcagaatatc gggcggatca cctgccatgc ggttcctttt aaggcggttc tccaacacct
     4441 ggatagacgt ggtcataatc tctgttgccg ttggcgctgt cacagcccga cgtgtcgtta
     4501 tgctgcctaa cctttctttc agacgcgcat gccacggtag agaatcacca ttctcgctat
     4561 tttcttcact gacattaaag gagagcagat cttgttgcat caaatgagca tcgtgctgca
     4621 ggtcgaccgc tatcacaata tcagccccca atgcacgcgt aagagaaata ggaattgggt
     4681 taactactgc cccatcaacc agccagtagc cgttatgtgc aacgggtgcc atgagtcctg
     4741 gaatactgca tgatgcgcga atagcaagat ggagatcgcc ttcagtaaac cataattcac
     4801 gccccgtact taaattggtg gcaacagccg caaagcgacg ggaacaattt tcgatctctg
     4861 tttccggcat tatttcgcga tattgattga agacacgctc gccgcgcagt aacccaccgc
     4921 gctgccagga gaggtccatc aggcgtaaaa cgtcccaata actgaaagag gttacccaat
     4981 cttccagcgc agataatcga tcgcatgcat aggccgcgcc caccagcgaa ccaattgaac
     5041 atcctgcaac gatatcaatt tcaataccca ctttttttag cgcattaata acgccaatat
     5101 gcgaccaacc tctcgccgcg ccagatccca gcgccagccc tatttttatc tttctcatta
     5161 ttcccgcaag attcccctgg aatgcaatcg tagccacatc gctactgctc ggttaacata
     5221 gtgctactca ggcggttaat acgccgttat tgtttccagg gagattattt gtgtctcagc
     5281 tttgtccctg tggtagtgct gtcgagtata gcctatgttg ccacccttat gtgtctggtg
     5341 aaaaggttgc acctgatcca gaacatctca tgcgttcgcg ttactgcgct tttgtgatgc
     5401 aagacgctga ttatttaata aagacctggc atccctcttg tggagcagcg gcgttacgtg
     5461 ccgaattgat gaccggattt gcgcatactg aatggttggg attgaccgta tttgaacatt
     5521 gttggcagga cgccgacaat atcggatttg tcagttttgt tgctcgattt accgaaggtg
     5581 gcaaaacagg tgcaataatt gaacgttcgc gatttttgaa agaaaacggt caatggtact
     5641 atattgacgg tacacgtccg cagtttggtc gcaacgatcc ctgcccttgt ggttcaggta
     5701 aaaaatttaa aaagtgctgc ggccaataat ggttgacggt acggtttagc aaacactctc
     5761 aacaaggttt ttccagcaat gcattcactc caacgtaaag ttctgcgtac tatttgtccg
     5821 gaccaaaaag gtctgatcgc acgtattacc aatatttgct acaagcacga gttaaatatc
     5881 gtacagaaca atgaatttgt tgatcaccgt accgggcgct tttttatgcg cacggaactg
     5941 gaagggattt ttaatgattc caccctgctg gcggatctcg atagcgcatt gccagaaggc
     6001 tccgtgcgtg agctgaatcc tgccggtcgt cgccggatag tgattctggt cactaaagaa
     6061 gcgcattgcc ttggcgattt gttgatgaaa gccaattacg gcggtctgga tgtcgaaatc
     6121 gcggcagtta ttggtaacca cgatacttta cgttctctgg ttgagcgttt tgatattccg
     6181 tttgagctgg taagccatga agggttaagc cgcaacgagc acgatcaaaa gatggcggac
     6241 gccattgatg cttatcaacc tgactatgtg gtgctggcga agtatatgcg ggtattaacg
     6301 ccggaatttg tggcacgctt cccgaataaa atcatcaata ttcaccattc attcctgcct
     6361 gcgtttatcg gcgcacgtcc ttatcaccag gcctatgaac gtggtgtgaa gattattggc
     6421 gcaaccgctc actatgtgaa tgacaatctg gacgaaggcc caatcatcat gcaggacgtt
     6481 attcatgtcg atcataccta cacagctgaa gatatgatgc gcgcaggtcg tgacgtcgag
     6541 aaaaacgtct taagtcgtgc gctatacaaa gtactggcgc aacgcgtctt tgtttacggt
     6601 aatcggacga ttattcttta atcgccagca aaaataactg gttaccttta atccgttacg
     6661 gatgaaaatt acgcaaccag ttcatttttc tcaacgtaac actttacagc ggcgcgtcat
     6721 ttgatatgat gcgccccgct tcccgataag ggagcaggct agtaaaaagc attaccccgt
     6781 ggtggggttc ccgagcggcc aaagggagca gactgtaaat ctgccgtcat cgacttcgaa
     6841 ggttcgaatc cttcccccac caccatcact ttcaaaagcc ccggaattct caaacgaatc
     6901 tgcaatcaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa ggttcgactt
     6961 gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc gcgcagcgct
     7021 gagtaatcct tcccccacca ccatcgcttc cttgaataat caaaacctgt tactccgagc
     7081 taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct gtaggtcgga
     7141 taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac cccgccgaag
     7201 cagggtctat gtgaactaaa attcgtttag tgacgagcgc gcaccagctg gtacttacgt
     7261 gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg gaacagcagg
     7321 aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc atcgaggtgt
     7381 tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag tttcatcatc
     7441 tcgctaccgt ccatatgctg agcggagaac ggaatggtca gcaggcccag cgcgcactgg
     7501 ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg cacgcgtggg
     7561 ctgaacatac gacgtttcag caaaagcacg ccgccaatca gacacagcac gccgctggca
     7621 ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc ttcatacatc
     7681 cagtgcggcg tcaacatacc gaagaagtga ccgacaaaaa tccccagaat accgatatgg
     7741 aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga cgccgcgcgc
     7801 caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac cgccccggct
     7861 atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc tgtcctccgg
     7921 tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga
     7981 ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct tcccagacag
     8041 catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc gccactttgt
     8101 cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg gcataacggc
     8161 tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata tctttcaaac
     8221 cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc ggcagatggt
     8281 ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca
     8341 ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc aacagtgacg
     8401 tggcacggcc acggtcgaac agttcgctgt actggacctg ggcatcgagc ggatccatcg
     8461 ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac agatttttcg
     8521 acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca tccggatatt
     8581 caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt tgctggtcac
     8641 atcgatggca tcgatacgac ggctgttgaa cagattgaat ttggtatctg agccgtggca
     8701 gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc gtgccagttc
     8761 acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca gataacggta
     8821 catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg tatcaacttt
     8881 accgtcaaca gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt tcagcgcacg
     8941 cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta ccggaatacg
     9001 caagctttcg acgtctggca gaattccgtt gctgcccagc tcgcccgcgt ctgctgcaga
     9061 ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat attctggatg
     9121 cagcggcagc gccagtttcc attccatcgc cattttataa accggcgact gctgtgcagc
     9181 ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt
     9241 cggatcgagg aacacgtcca gctgacgctg gtagagatct ttctcgttct cggtgctggc
     9301 tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga tacgaccgac
     9361 acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc agaagatgca
     9421 cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc atccggtgat
     9481 gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat cttcttcacg
     9541 cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc agtgttcgca
     9601 caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg ccttctggat
     9661 gttgtcgaag ttcttgtctt tcgccagttt atcaaactca ccgcccaggt catcttccca
     9721 gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac gcggacgggc
     9781 aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgataat caaaatcgaa
     9841 cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcaaagattt tacccagcag
     9901 catggctcgg ttacccatgc gcggttgcag tttgccattg attttacgga tccagccgcc
     9961 tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct tggtttccac
    10021 gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt tacaggtaac
    10081 tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga cttgtgaacg
    10141 aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg
    10201 ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt gccgtagtag
    10261 ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt cggcgtgata
    10321 cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc aggcaggtta
    10381 acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg ctggctgaca
    10441 accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc gttatcggcg
    10501 atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc gcgacctaaa
    10561 gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg gtgcggcgtc
    10621 aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac ttctttcacc
    10681 gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc acgcatccac
    10741 tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat cagctcgtga
    10801 acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga ccaggtcggg
    10861 ctggagataa ttttgcgcgg ctgtgcctga atatcgcgga agcggatctt ctcgtcttct
    10921 ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc agcccaggct
    10981 ttcacggcta cctgaccgtt ggtttccggt gccagcgtca ggatcatctc tgccgcatca
    11041 attgcggtgt tcagcatcgg ctggcctttt gccggacctt ccgctttggt gtagttgagc
    11101 ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc gccgttaccg
    11161 attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgccgg ataatcgcgc
    11221 tctacgacca taatgtgcgg tgcagttttg cctgggatca ggtcgcattc gccttttttc
    11281 cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcgtgctg gataggcagc
    11341 gtgacgatgt cggtttcttt acccagatag ccgacgcaca cttcggagaa tttcttcgcg
    11401 atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac cgccgcagac
    11461 agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc gtaccaggtc
    11521 gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa gtccagcgta
    11581 accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc ttccggcttc
    11641 acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc cagcaggtac
    11701 ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg ccagatgaac
    11761 aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg gatggaaccc
    11821 tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc cgcttcgcca
    11881 gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc catgcgttcc
    11941 gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc catcggtgac
    12001 agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt atagaaataa
    12061 gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc
    12121 cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc gcccccgctc
    12181 tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt catatcgagg
    12241 tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg cgttttatca
    12301 gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac gccggtaatc
    12361 tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc acagttaacg
    12421 tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac ggtggtcacc
    12481 agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg
    12541 ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta cgggaagcct
    12601 acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt ttcttcgcca
    12661 gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg gaagccaata
    12721 gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt ccattccgga
    12781 ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg acctgcagcg
    12841 tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta gcgacgcaca
    12901 tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat tacgtggccc
    12961 atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag atcgcacagt
    13021 ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt gtaacgcact
    13081 tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc ccaggcgatg
    13141 atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc gccccaggtt
    13201 tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca agtaccgcca
    13261 atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc cggaattggc
    13321 gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg
    13381 atcagttcgt tcacctcctg ccaagaagaa cgaacaaatc caccgcgtcc acgcgcctgc
    13441 ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc aaccggatcg
    13501 ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat catcgggtat
    13561 ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg gcagccgcga
    13621 ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg ggtttcccag
    13681 gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca gtttaccccg
    13741 tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc ctcccagtca
    13801 cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt ctgtttgaag
    13861 tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc tgtcggccct
    13921 ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa
    13981 ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag ataccccttt
    14041 aggataatta acagttacag cataacatgc tgtaaattaa gacgttttat acctatagtt
    14101 atttaaacta ttaagatttt tatgcaaatg ggtattaagg agtattcccc atgggtaaca
    14161 tattaatggc tcttaatgaa atccggcgaa ataagccata taacactaat atgttgtaac
    14221 taaatgacgc ataaaaaaag cgcggcatca tgccgcgcaa aggataatca aatattaaga
    14281 tttatttttt agaatgccga ccatataccg cccaagtaat cactacgcag gcgatataga
    14341 aaatcaaaaa tactttcatt gcacctactg gcgaacccgt taatgccagc gagctaccaa
    14401 acgctttcgg gataaagaag ccaccaatcg cgccaatcgc agagatgaaa cccagcgccg
    14461 ctgccgtgcc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct tctgctttca
    14521 cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg gaaccactac
    14581 ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg aagcttccgc
    14641 cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc atcagaataa
    14701 agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca cctgcagaac
    14761 gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga acatccggga
    14821 actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gatgaaggag ccgaaggttg
    14881 ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc aactgctcct
    14941 tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca atggtgaaga
    15001 tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca gtcccatccg
    15061 gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc accagcggag
    15121 caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga cccagcgcgc
    15181 caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat gcgaagttcg
    15241 cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc gtggaggtat
    15301 cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg ctgaacgccg
    15361 tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt aataacgcgc
    15421 cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa ttaaaaccga
    15481 ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac gccagcagca
    15541 gacagggaac ggaaatccac aggttgcggc tggcaatacg ttgaccgcgt tgttgccaga
    15601 acgcaggatc ttccggtcgc caatctgtta tgacagctcc agtagccctt tcggaggcag
    15661 atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt agtgtctgga
    15721 gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca ttgatattta
    15781 tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc acggtgctgt
    15841 agaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag gagtaactct
    15901 ttccggctat gggtatactt cagccaatag ccgagaatac tgccattcca gaatatatcg
    15961 tcacattcat taaggttatt gctcatttaa agcctgaagg aaggggttta catgcttaaa
    16021 cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt gctttctact
    16081 gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt tcagggcagc
    16141 gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct gttggcggca
    16201 gtgccattaa gcgagaaaga caagccctta attaaagaga tggaacaaac ggcatttagc
    16261 gccgagttga ctcgagcagc agaacgagac ggacaactgg cgcaattaca gggcttacaa
    16321 gattactggc gtaatgaact gatccctgcg ctgatgcgtg cacaaaaccg agaaacggtg
    16381 tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg ttttgaccgc
    16441 accacggaaa tgcgtattga gacagtggta ctggtccatc gggtaatggc ggtatttatg
    16501 gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca accgtggcgg
    16561 caactgctgg caatggcgag tgccgtcagc catcgcgatt ttacccaacg cgcaaacatc
    16621 agcgggcgca acgaaatggc gatgcttgga actgcgttga acaatatgtc tgcagaactg
    16681 gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg gctggagcat
    16741 aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc ccgcgccccg
    16801 ctgtgtgaac gcctgtcacc ggtactcaac ggcttacaga atttaaccct gctacgtgat
    16861 atcgaactgc gggtgtatga cactgatgat gaagagaatc atcaggagtt tacctgccag
    16921 ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt attacccgtt
    16981 ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca gtacggtatt
    17041 ttgctggcga ccctgccgca ggggcgtcat cttagccatg accaacaaca actggtggat
    17101 accctggttg aacaacttac cgccacgctg gcgctggatc gccatcagga acgtcagcaa
    17161 cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga ttctattgcc
    17221 caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg cgatgcgctg
    17281 ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc atcctgggcg
    17341 cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg attacgtccg
    17401 gcgctggaag cgagttgcga agagtacagc gccaaatttg gcttcccggt gaagctggat
    17461 tatcaattgc cgcctcgtct ggtgccttca catcaggcaa tccacttgtt gcaaattgcc
    17521 cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaggtcgt ggtgacggtg
    17581 gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgtgg cgtgcctgaa
    17641 aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc gcaaagttta
    17701 cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt ggtcaccttt
    17761 attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc aggaaccggc
    17821 tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac agcttatcag
    17881 tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg gtattgaact
    17941 ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg gcatgaacgg
    18001 tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg tggtattcag
    18061 cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg atggctatct
    18121 gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag ctgctggcga
    18181 aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg ctaaccgtgc
    18241 cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc tcaagctgat
    18301 tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg aaagcacagt
    18361 aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg tggaagcagc
    18421 ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt tcgtcgtttg
    18481 acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag acacgctgcc
    18541 cctggttatc ttccaccacc acagacaatc gccagtgatt gcttgcccct tccccgttct
    18601 gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg cccggcgtca
    18661 aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa cgactgcgga
    18721 tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt gtcgccagaa
    18781 acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt cgctgcggat
    18841 tgtcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca gaaagttgtt
    18901 ttttgagcgg tacaccaaag cggtaattaa gattcagccc gaggttattt tgattttcgc
    18961 cactttcccc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg gtgtaattta
    19021 atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac aaatcaacac
    19081 gatcaccaaa atactgttct aggctgacac tggtattgag gtgttgatag aacggcatgc
    19141 gcatccgagc tgtcaggtcg tacccgcgcg ccatccgttg ttcctgcgtg gctgtctgtt
    19201 catgccatgc agcaaacggc tgataaaagt ttgccgataa tcgcaaatat tcgccccacg
    19261 cttcggcacc aaagcccgct cgctgaagat tttcgtcctg caagttgtca taaaaagtgt
    19321 tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca ttgctcacca
    19381 acccattatc ctgctgagta agaccaagct ggctccaggt gagataacga tcattatctt
    19441 gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta tccactttga
    19501 cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga ttaacctgtt
    19561 gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg ccagtatcca
    19621 gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca aagtgttttt
    19681 ccccatcatg attttcggga gccatgccta aatccggcag accatcattg ttattatcaa
    19741 agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg cctgctacaa
    19801 gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg tcggtaactt
    19861 ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac ttttgtcgta
    19921 ttttctcgcg ctttcaggaa aatccttaaa aatgtgaccg attggtcaga cgctgcagaa
    19981 gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata atgcaaaaaa
    20041 tagtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac agcttgcggc
    20101 tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc ctgatgtctg
    20161 atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac attcagcaaa
    20221 tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc tgtaccgacg
    20281 ggcgcgggat tagcacactt cctctgattg atggggtgga aatcggtact cttgtggaac
    20341 tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgttta agggggcgtc
    20401 tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccttaaca caccatccgg
    20461 ataattctcc gccagtagtt tttttaccga taccagcaaa tcattcagcc catcgtcctg
    20521 cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcat tggttcccag
    20581 cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt cagattcata
    20641 ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc catcatcaag
    20701 atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct tccacaacag
    20761 ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg tggtgcgtcc
    20821 gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc cggcggtcag
    20881 gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcatgatt cggtaaactc
    20941 cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc cctcatcagg
    21001 tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc ataagagtga
    21061 ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat cacgcgttat
    21121 caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt cttccggaca
    21181 tttttaccgc tcattttgaa accactaacg gaacaatatg caatccacgg accacttgca
    21241 aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt ttatgccatt
    21301 tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact ttatgcgcgg
    21361 tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga tcccatgcgc
    21421 tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc tttacgcttt
    21481 ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa gttgaacatt
    21541 aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt cagactgttc
    21601 ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg ccccaatgag
    21661 taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag acactttctg
    21721 ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg gataaccata
    21781 atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct tattttcccg
    21841 gttctggtgc tggtagtgct gttcctgtgg ggaagtagcc agacactacc agtggtcatt
    21901 gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt tgtccgtcat
    21961 gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct tagcctttca
    22021 gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga cgccgcgcca
    22081 acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg gctggttggc
    22141 ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct gtttggtatc
    22201 aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt tccaatggct
    22261 ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat ttctgcggca
    22321 atgtatggcg tatttttgct gatccaggcc aaaacgcatc aaagtttgtt tgtctacgag
    22381 cacgaagatg acagtgatga cgacgacccg caccacggta aaacgtctgc ccatagcagc
    22441 ctgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt caccaaaatg
    22501 aacgccagct cgctggagac attgctcgac agcatgaatg cccctgttgc ctttactggc
    22561 ttcctggtgg cactgttgat tctgtcgccg gaaggtttag gtgcgttaaa agcagtgttg
    22621 aacaaccagg ttcagcgcgc gatgaatctg ttctttggtt cagtgttagc aacaatttcg
    22681 cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt gcagtttgca
    22741 cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca tatctccttc
    22801 tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt tgccgcctat
    22861 ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc gggagagata
    22921 agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt aatcagaatt
    22981 gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa gccggaatgt
    23041 actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt agtatccaca
    23101 ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    23161 gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac aaaacggggg
    23221 ctaatcttga aaccagacac ttcaagaata gccgcaaaat gttgtcatta caacaaggcg
    23281 gctatatgac gctcgcgcag tttgccatga ctttctggca cgacctggca gcaccgatcc
    23341 tggcggggat tattgccgca gcgattgtca gctggtggcg taaccggaag taaggtgaaa
    23401 acgggcgtca ggctgccgtg atggcaatat gcgcccgaaa ccggccgcag gggtaacagc
    23461 ctgcggcctt ttttgtatct ggcacccatg aaaaaagtct taacgcagaa cgctaagact
    23521 ttatttttca agcaaaaaag attacttgct ggtatccagt tcttcgaaac ctttcaccag
    23581 atcatcaatc gctttcatct gcttgaggaa cggttccagt ttagccagcg gcagcgcgga
    23641 tggaccatca catttcgcat gttccggatc cggatgcgct tcaataaaca gccccgccag
    23701 acctaccgcc ataccggctc gtgccagctc agccacctga gcacgacgac caccggaagc
    23761 tgcgccaaac ggatcgcggc attgcagtgc gtgggtcacg tcgaaaatca ccggcgagtt
    23821 accagacact ttcttcataa tgctgaagcc cagcatatcg acaaccaggt tgtcatagcc
    23881 gaagttagca ccacgatcgc aaagaatcac tttttcgttg ccgccttctt tgaatttatc
    23941 aacgatatta cccatctgtc ccgggctgac aaactgtggt ttcttgacgt taattaccgc
    24001 accggttttc gccatggctt caaccaggtc agtctggcga gcaagaaacg ccggcaactg
    24061 aatcacatcc acgacatcag caacgggctg tgcctgactt ggttcgtgaa cgtcggtgat
    24121 aattttcacg ccaaaagtct gcttcaactc ctggaagatt ttcatccctt cttccaggcc
    24181 cggtccacga taagagtgga tggaggagcg gttggctttg tcaaaagagg ctttgaacac
    24241 gtaagggata cccagtttct gggtcacagt tacgtagtgc tcgcaaatgc gcatcgccag
    24301 atcgcgagat tccagcacgt tcataccgcc aaacagtacg aacggcaggt catttgctac
    24361 gttgatgtcg ccaatgctaa ccactttttg tttcatagga tcgccttaat cttgagtaaa
    24421 atgtcgatta attaatgcag cacaatatgt ttatgcgcga tgttatttat ttgcgcacgg
    24481 atcatttcgc tgatcgggtc ttccggacac cgttcaacga aataacttaa atcgttcaac
    24541 gcaacgtgtt cgcaatccag ttgcgcataa atcaacccac gatcgcgaat ttcataggga
    24601 tcttcagggt tgaattgtaa taaagcttcg ctggtgcgta acgccagctc catctgattt
    24661 tcttccatca acgaggcttt gagtgtatcc agcaatttgc ggattacctc aatgttatca
    24721 gcttcatcaa ggtcttcata aaacagttcc gccgacgggc tgatatttcc ctttaaccat
    24781 ccgtccagca tatgttcgct taacgattca ccgttaaaag gattaatcag ccaaatttcg
    24841 ccatccggac attcaatgcg caatatcagc tgcgtaggga aaatcaccgg cagcagcggc
    24901 aaatcgagac gattcgcgac ccataataaa accgcaccta atgataccgc actgccctgt
    24961 cgattcttta acacctggtc cagccataat gcatcggaaa gacgataaac gccgcgtgag
    25021 gctttaaatc cccagtcgcc gtaaaacagc gcgatcagtt tttccagctg ctcttctaaa
    25081 ggcagaagct ggctgatttc ttccttcgcc agactaacga gacgctccag ttcgtcgtaa
    25141 acatcttgcg agggaaaatc gcggcggatt gcttcgcaag ccaggatcat gccttcgcac
    25201 aatggcgctt tattaaattc gaaatcagct aacgatctca tgacttaccc cagtaacggt
    25261 acttttgtgg tggcgagttt aatgatgatg tacagcacca ccaacgccag cgggaaggca
    25321 ataatgcgcg cctgctgact gtgcatacga cgataatcga gtgcaataaa acccaaaacg
    25381 atataaatga taactccaaa cagcttttca gtcagccact gtgcctgccc ggaaaatggc
    25441 aggatgtgcg ctttagccat caacgcaatg ccgcttaaca ataacaccgt atcgacaacc
    25501 ggcggcacaa tgcgcgtcca gcgagcaaat gcctgtggat gcttctgata acgtagccag
    25561 aaacgtaagg ttaatagccc aacagaaagc gcgatactaa taagatgaac actaagcagt
    25621 gtagaaaaac ttgtcattga taatagcggc cgagcgttac gcgctcgtta tcaccatagt
    25681 cacggcaggt ttcgacgtca tgataccccg cgaggataaa tgcttgtcgc accgcttcgc
    25741 cctgctgcca gccatgttcc agaagcagaa agccgccgga taccagcgcg ttacgcgact
    25801 gttcgatgat atgcacgatg tctgccattc cactgtctgc cgcaaccagc gcagtgagcg
    25861 gctcaaagcg gacatcgcct tgttgaagat gtgggtcctg ctcgtcaata tacggcggat
    25921 tgctgacaat catcgcaaac tgctgcccgg ctagcgcgct aaaccagtcg ctttgcagaa
    25981 tgtggatatt tttgatcgcc agatgctggg cattacgttg tgccagggag acagcatcag
    26041 gcatacgatc tacagcgata atttcgcagt ccgggcgctc gctagccagc gcaagcgcaa
    26101 tcgccccggt acccgtcccg agatcgagaa tacggcaagg ttgttcaggc aaccgcgcca
    26161 gtgcctgctc caccagacac tccgtatccg ggcgcggaat taaggtcgct ggcgaaacaa
    26221 ataacggcaa cgaccagaat tctcgcaccc cggttaaatg agcaatgggt tcaccatcgc
    26281 gacgacgtgt cagtagcgca tcaagttgct gacattgttc gtcagtcagc tgcgtttcac
    26341 caaaggcgag aataaaagta cgccctctgc cggtaacatg ctccagcagg atttcagcat
    26401 cacgccgcgg gctttcgctc gcctgaagtt ggcttattgc ttcacgtaac cagtgttgat
    26461 attccattat tcctgctcgg acaacgccgc cagttggtcg gcctgatgtt cctggataat
    26521 cggttcaatc agcatatcca gcttaccttc catcacttca tccaggcggt agagcgtcag
    26581 gttgatgcgg tgatcggtaa cgcgcccctg cgggaaattg taagtacggt tacggtcgct
    26641 gcgatcgcca ctccccagca ggttacgacg ggtagacgct tcggcctgtt ggcgttttgc
    26701 catttcagca gcgtggatgc gagcaccgag cactgaaagc gccttcgctt tgtttttatg
    26761 ttgtgaacgt tcgtcctgac attcaacaac aatcccggtc ggcaagtgag taatacgaat
    26821 tgccgaatcg gtggtgttaa cgtgctgacc acccgcccct gacgagcgga aagtatcaat
    26881 gcgtaaatct gctgggttga tgtccggcag ttctgcgtca ggcagttctg gcattaccgc
    26941 aacggtacaa gcagaagtat gaatacgacc ctgcgattcc gtagcaggaa cacgttgcac
    27001 gcgatgaccg ccggattcaa atttcagacg accatacaca ccatcaccgc taattttggc
    27061 gatgatctct ttataaccac catgttcacc ctcgctggcg ctcatgattt ctacccgcca
    27121 gcggcgggct tcggcgtaac ggctgtacat acggaacaga tcgcctgcaa acagcgctgc
    27181 ttcgtcgccg ccggttccgg cacggacttc gaggaaggcg ttacgttcgt catcaggatc
    27241 ttttggtagt aatagaacct gtaattgctg ttccagttgc tcgctttttt ctttagcttc
    27301 gcgcagttca tcctgcgcca tctcacgcat ttcaggatcg tcgagcatca tctgtgcggt
    27361 ttcgatatct tcctgaacct gttgccagtc ggtaaaacag cgcgaaacat cacttaactg
    27421 cgcatattcg cgtgataatg cgcgaaaacg ttcctggtcg gcgatagttt gcgcgtcacc
    27481 cagcaacgcc tgaacttctt catggcgttc atgcagggct tccagtttgg caacgataga
    27541 aggcttcata ggcgtaaatg caccctgtaa aaaaagaaaa tgatgtactg ctactccagc
    27601 ccgaggctgt cgcgcagaat attcaggcgt tcgttatccc cgtcacgggc ggcctgttga
    27661 agtgatttcg ttggcgcatg gatcaagcgg ttagtcagtt tccatgccag atcctgcata
    27721 atggcttgcg cgtcgccgcc ctgctcaagg gccgctaacg ctttggcggt taactcatcg
    27781 cgaacgtgct ctgcctggct gcgatactcg cgaatggttt cgctggcgct ttgtgctcgc
    27841 agccacgcca taaattcgct ggtttcctga gcgacaatag tttccgcctc aaccgctgcg
    27901 gctttacgct gcgccaggtt gtgcgaaatg atgctttgca gatcgtccac actataaaga
    27961 taagcattcg ccagtttgcc aacttccggc tcaacatcgc gcggaacggc aatatccacc
    28021 aacagcattg gttggttacg acgacttttt aatgcgcgct ccaccatgcc tttcccgata
    28081 atcggtaacg ggctggcggt ggaactgatg atgatatcgg cttcgcgcag acgttcgtcg
    28141 atctcactca gggcaatcac ttccgcgcca acttcatctg ccagaatttg ggcacgttcg
    28201 cgagtgcggt tggcgataat catcttctgt actttatgtt cgcgcagatg acgcgctacc
    28261 agctcgatgg tttcgcccgc gcctaccagc aacactgtga ccgtagagag cgattcaaag
    28321 atctgccgcg ccagcgtaca agccgcaaaa gcgacagaca cagcactggc accgatatct
    28381 gtttcagtgc gaacgcgttt cgctacagag aaagatttct ggaacatgcg ttccagttcg
    28441 ctggccttca tatgaccttt ttgcgaatcg acaaacgctt ttttaacctg accgaggatc
    28501 tgcggctccc caagaaccag tgaatccagg ccgctggcaa cacgcattaa atggctaacc
    28561 gcgtcgttat cctgatgcca gtagaggctt ttacgcagat cttcttcatt aagattgtga
    28621 taatcgcaaa gccagcggat taacgcctct tgcaggttat cctgctcttc aacgctaaga
    28681 taaagttccg tgcggttgca cgtcgacagc accacgccgc cctgcaccat cggctgcgca
    28741 agcaggctgt caagcgcctg atcgagctta tccggcgaaa acgatacacg ttctcgcagc
    28801 gatacaggtg ccgttttatg gttgataccg agtgctaaaa gggtcatgtc tgcgggaaat
    28861 aataccaacg ttgatagggt tagtctgctt gcatcataca ggatgcgtaa gatcaataaa
    28921 agagagcgcc cccttttgga gtaattgccg aaagccgtta gattctggca attaagacaa
    28981 cttgaacata gacgatagcg gacggtaacg ctagcattaa gggttataac tgcaacgtat
    29041 ctcaaggact tgtcatcact atgcccctgc ccgattttcg tcttatccgc ctgctaccgc
    29101 tggctgctct tgtgctcact gcctgttccg ttaccacgcc caaaggtcct ggcaaaagcc
    29161 cggattcgcc acaatggcgt cagcatcagc aagacgtgcg caatcttaat cagtatcaga
    29221 ctcgcggcgc gttcgcttat atttctgacc aacaaaaagt gtacgcccgc tttttctggc
    29281 agcaaaccgg ccaggatcgc taccgtctgc tgctcactaa cccattgggc agcacggaac
    29341 tggagctgaa tgctcaaccg ggtaacgtgc agttagtcga caataaaggt cagcgttata
    29401 ccgccgatga cgccgaagag atgattggca aattgaccgg aatgccaatt ccgctcaaca
    29461 gcttgcgcca gtggatttta ggtttaccgg gtgatgcaac cgactacaaa ctggacgacc
    29521 agtaccgcct gagcgaaatt acctacagcc agaatggcaa aaactggaag gttgtttatg
    29581 gtggttatga caccaaaacg caacctgcga tgccagccaa tatggaactc accgacggtg
    29641 gtcaacgcat caagttaaaa atggataact ggatagtgaa ataatgcgga cacagtggcc
    29701 ctctccggca aaacttaatc tgtttttata cattaccggt cagcgtgcgg atggttacca
    29761 cacgctgcaa acgctgtttc agtttcttga ttacggcgac accatcagca ttgagcttcg
    29821 tgacgatggg gatattcgtc tgttaacgcc cgttgaaggc gtggaacatg aagataacct
    29881 gatcgttcgc gcagcgcggt tgttgatgaa aactgcggca gacagcgggc gtcttccgac
    29941 gggaagcggt gcgaatatca gcattgacaa gcgtttgccg atgggcggcg gtctcggcgg
    30001 tggttcatcc aatgccgcga cggtcctggt ggcattaaat catctctggc aatgcgggct
    30061 aagcatggat gagctggcgg aaatggggct gacgctgggc gcagatgttc ctgtctttgt
    30121 tcgggggcat gccgcgtttg ccgaaggcgt tggtgaaata ctaacgccgg tggatccgcc
    30181 agagaagtgg tatctggtgg cgcaccctgg tgtaagtatt ccgactccgg tgatttttaa
    30241 agatcctgaa ctcccgcgca atacgccaaa aaggtcaata gaaacgttgc taaaatgtga
    30301 attcagcaat gattgcgagg ttatcgcaag aaaacgtttt cgcgaggttg atgcggtgct
    30361 ttcctggctg ttagaatacg ccccgtcacg cctgactggg acaggggcct gtgtctttgc
    30421 tgaatttgat acagagtctg aagcccgcca ggtgctagag caagccccgg aatggctcaa
    30481 tggctttgtg gcgaaaggcg ttaatctttc cccattgcac agagccatgc tttaagccgg
    30541 gcaagctgag tttcggtgac aacgtcacct tgttccagac gttgcatcgc gctctttaat
    30601 acaccgcctg gaaaggatca tgcctggccc gcacagtttt cggcagattc tttccaccaa
    30661 tggacgcatg cctgaggttc ttctcgtgcc tgatatgaag ctttttgctg gtaacgccac
    30721 cccggtacta gcacaacgta ttgccaaccg cctgtacact tcactcggcg acgccgctgt
    30781 aggtcgcttt agcgacggcg aagtcagcgt acaaattaac gaaaatgtac gcggtggtga
    30841 tattttcatc atccagtcca cttgtgcccc tactaacgac aacctgatgg aattagtcgt
    30901 tatggttgat gccctgcgtc gtgcttccgc aggtcgtatc accgctgtta tcccctactt
    30961 tggctatgcg cgccaggacc gtcgcgtccg ttccgctcgt gtaccaatca ctgcgaaagt
    31021 ggttgcagac ttcctctcca gcgtcggtgt tgaccgtgtg ctgacagtgg atctgcacgc
    31081 tgaacagatt cagggtttct tcgacgttcc ggttgataac gtatttggta gcccgatcct
    31141 gctggaagac atgctgcagc tgaatctgga taacccaatt gtggtttctc cggacatcgg
    31201 cggcgttgtg cgtgcccgcg ctatcgctaa gctgctgaac gataccgata tggcaatcat
    31261 cgacaaacgt cgtccgcgtg cgaacgtttc ccaggtgatg catatcatcg gtgacgttgc
    31321 aggtcgtgac tgcgtactgg tcgatgatat gatcgacact ggcggtacgc tgtgtaaagc
    31381 tgctgaagcg ctgaaagaac gtggtgctaa acgtgtattt gcgtacgcga ctcacccgat
    31441 cttctctggc aacgcggcga acaacctgcg taactctgta attgatgaag tcgttgtctg
    31501 cgataccatt ccgctgagcg atgaaatcaa atcactgccg aacgtgcgta ctctgaccct
    31561 gtcaggtatg ctggccgaag cgattcgtcg tatcagcaac gaagaatcga tctctgccat
    31621 gttcgaacac taatcgaacc cggctcaaag acccgctgcg gcgggttttt ttgtctgtaa
    31681 tatccatttg tatgacctat gcctccttca cctgccattt agttgacaga tgatgcgctc
    31741 acggatgaaa cattattgtg aacaaaatat tttcctcaca tgtgatgcct ttccgcgctc
    31801 tgatcgacgt ttgctggaaa gaaaaatata ctgccgcacg gtttacccgt gacctgattg
    31861 ccgggataac cgtcgggatt attgctatcc cgctggcgat ggcgttggct attggtagtg
    31921 gtgtggcccc ccagtacggt ttatataccg cagctgttgc ggggattgtc attgctctaa
    31981 cgggtgggtc acgctttagc gtttccggtc cgactgcggc atttgtggta attctctatc
    32041 ccgtttcgca acagtttgga ctggcaggac tgctggttgc gaccttgttg tcgggaatct
    32101 ttttgattct gatggggctg gcacgctttg gtcgtctgat tgagtatatt ccggtttccg
    32161 tcaccttagg tttcacctcg ggtatcggga tcaccatcgg taccatgcag attaaagatt
    32221 ttctcggtct gcaaatggcc catgtcccgg aacattatct acaaaaagtc ggcgcattat
    32281 ttatggcgct gccgaccatt aatgtgggtg atgctgccat tggcattgtg acgcttggca
    32341 ttctggtttt ctggccgcgt ctgggcattc gtttacccgg tcaccttccg gccttgctgg
    32401 ctggttgcgc ggtgatgggg attgttaacc tgctcggcgg acatgttgct accatcggtt
    32461 cgcaattcca ctacgtcctg gccgatggtt ctcagggtaa cggtattccg caactgctgc
    32521 cgcaactggt gctgccgtgg gatctgccta attcagaatt cacgctaacc tgggattcta
    32581 ttcgcaccct gctgcctgcg gcattctcaa tggcaatgct cggggcgatc gaatctctgc
    32641 tctgcgctgt ggtactggat ggtatgaccg ggactaagca caaggcgaat agtgaactgg
    32701 tcggtcaggg gctggggaat atcatcgctc cattctttgg tggtattacc gctactgctg
    32761 ccatcgcgcg ttctgccgct aacgtccgtg ccggggcaac ttcccctatc tcggcggtga
    32821 tccactctat tctggttatt ctcgccctgc tggtactggc accactgctc tcctggctgc
    32881 cgctttccgc catggcagcc ctgctgttga tggtggcgtg gaacatgagt gaagcgcaca
    32941 aagtggtcga cttgctgcgt catgcgccga aagatgacat catcgtcatg ctgctgtgta
    33001 tgtcactgac cgtgctgttt gatatggtta ttgccatcag cgtggggatc gtgctggcat
    33061 cgctgctgtt tatgcgtcgt atcgcacgta tgactcgcct ggcaccggta gtcgtagatg
    33121 ttccagacga tgtcctggtt ctgcgcgtta ttggcccgct gttttttgct gctgctgaag
    33181 gcttattcac ggacctggag tcacgtcttg aaggcaaacg gattgtgatt ctgaagtggg
    33241 atgccgttcc ggtacttgat gctggtggtc ttgatgcgtt ccagcgtttt gtgaagcgtc
    33301 tgcccgaagg atgtgaactg cgcgtgtgca acgtggaatt ccagccactg cgaactatgg
    33361 ctcgcgcagg cattcaaccg atcccgggac gcctcgcgtt cttcccgaat cgtcgcgcgg
    33421 cgatggcgga tttataagta cgagattgac cagtcagcgc aaactgactg gtcagcaaac
    33481 tgcattattt gttagctatg acggcgatta tcgctacggc ggcaacgttt gtcatagtga
    33541 cgaacccacc agtaacggtc gcacacctgt tcatgcccgc caacacgggc acccgccagc
    33601 cagagaatgg caccgacgaa aatacttagc actgcaccat gtgcgaaata ctggggcata
    33661 ttaaactgtg gtaactggtt gaggattgaa taccccacgc cgaccaccat taccaccaga
    33721 cccaacccca tgagcacgtt accgagtatc gaagcgtttt tgcgtttcat atgtcacctc
    33781 cggaactttc tgggttgtaa cagggaatac ccctcttcct tatgtgtaaa gtatagacaa
    33841 caccctgtgg gttatgtgcg ggcgtaatca caattacaac ccttatttca acaaaacttt
    33901 acaaataaac gcctgaacac cttgactttc taatagtcca ctcaggcaat tattacgtta
    33961 tctgcatttg atacgcagtt ttttgtcaag cggggccgca accagtaaac tacgcgccag
    34021 ttatgtacac actcaggaca aaaaaacgtg acgattaaat tgattgtcgg cctggcgaac
    34081 cccggtgctg aatacgccgc aacgcgacat aatgctggtg cctggttcgt tgacttactg
    34141 gcagagcgtt tgcgcgctcc gctgcgcgaa gaggctaaat tctttggtta tacttcgcga
    34201 gtcacccttg gaagcgaaga tgtccgcctg ttagtcccga ctacatttat gaatctcagc
    34261 ggcaaagccg ttgcggcgat ggccagtttt ttccgcatta atccggacga aattctggtg
    34321 gcccacgacg aactggatct gcctcctggc gtcgccaaat ttaagttggg cggtggccat
    34381 ggtggtcaca atggactgaa agacatcatc agtaaattgg gtaataaccc taactttcac
    34441 cgtttacgca tcggaatcgg tcatccgggc gataaaaata aagttgtcgg ttttgtgtta
    34501 ggcaaaccgc ctgttagtga acagaagtta attgatgaag ccatcgacga agcggcgcgt
    34561 tgtactgaaa tgtggtttac agatggcttg accaaagcaa cgaaccgatt gcacgccttt
    34621 aaagcgcaat aagtcgttgt ctgcggcatt tttgccgagt gccgtgtata ataggcaaag
    34681 ttatttccat ttctgcaatc tgttagcaat aacaggttga ttattaagat attaaggtga
    34741 tttaaatcat gggattcaaa tgcggtatcg tcggtttgcc caacgtcggg aaatctaccc
    34801 tgttcaacgc gctgaccaaa gccggtattg aagcggccaa ctttccattc tgcaccattg
    34861 agccgaacac aggcgtcgta ccaatgcctg accctcgcct ggatcaactg gctgaaatcg
    34921 taaaaccgca acgtacgctt cccacgacca tggaatttgt cgatatcgcc ggtctggtaa
    34981 aaggcgcatc gaaaggcgaa ggtctgggta accagttcct gaccaacatc cgtgaaaccg
    35041 aagcgatcgg tcacgttgtt cgctgctttg aaaatgacaa catcattcac gtttccggca
    35101 aagttaaccc ggctgacgat attgaagtta tcaacaccga actggcgctg gcagacctcg
    35161 acacctgcga acgtgcgatt catcgcgtac agaagaaagc caaaggtggc gataaagacg
    35221 cgaaagctga gctggcggtc ctggaaaaat gcctgcccca gctggaaaac gcaggtatgc
    35281 tgcgcgcgct ggatttaagc gctgaagaga aagcggctat tcgttacctg agcttcctga
    35341 cgctgaaacc aacaatgtac atagccaacg tcaacgaaga tggttttgaa aacaacccat
    35401 atcttgacca ggtgcgtgaa atcgcggcga aagaaggttc tgttgtggtt ccggtttgtg
    35461 ctgctgttga agcagacatt gccgaactgg acgacgaaga acgtgacgag tttatgcagg
    35521 agcttgggct ggaagaaccg ggcctgaacc gtgtgatccg tgccggttat aagctgctga
    35581 acctgcaaac ttacttcacc gctggggtga aagaagtgcg tgcatggacc attccggttg
    35641 gagcaaccgc gccgcaggca gcgggcaaaa tccatactga ttttgaaaaa ggctttatcc
    35701 gtgcacaaac catctcgttt gaagatttca tcacttacaa aggtgaacaa ggcgcgaaag
    35761 aagcaggcaa aatgcgtgca gaaggtaaag attacatcgt gaaagatggc gatgtgatga
    35821 acttcctttt caacgtctaa ttaatttatt gtctcatgag gtttcgctaa atctcatgaa
    35881 gagcgcaaaa gtatataaaa tccacgcaat tgcgtggatt tttcatttca ccgagtccta
    35941 atcattcttg taacatccta cacttttgtg cttattgcct acagtgaacc acgagaataa
    36001 ttaaagacat aagtgttcag atgactgata tctcattagc agtgatataa cgtcagtttt
    36061 tgttccacta cacatcagcc ctcttcacag ctgatcaata ttgatctcgc tccaatatgc
    36121 tggcgagaaa aggacttacc tgaagggtaa tatcccgcag ataaagtccc cgaataacgt
    36181 gattacgtgc gatcctggca agatgaaaat gataaccagt agcacaataa taacgatagg
    36241 ccagaaaatc cattgaacaa aaatgaatat cgatgttttt tgctttttct tattagtaat
    36301 gcaaaaatgg cgcatattgt ttttgttatg cagatacatc aagcgaaaat cccataacta
    36361 attaggatta ttacaaaaaa tcttcaccaa attttttaac cgctgtttgt tgtaatattt
    36421 ttgccatatt catagtaacg aattggaata agtaacgaca acttagggaa taactcgata
    36481 taaactcacc tttgtaaggg gggggttaaa tatatattaa ttcctgtgtt cctgattttg
    36541 cccggtaata gagaattatt ctcagagaag ctcatcatga ttgcaccata cagatatcat
    36601 gatgataaga ggatattatt cattgactta aacaaaatga caaaatccat agacccatag
    36661 cctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat accaaagccg
    36721 atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa tttggcgcta
    36781 ttggtttgag tgcaattatc ttttcccccc tgctgatagc tgctgataca ggaagtcaat
    36841 atggcaccaa tattactatt aatgatggtg acagaattac aggagatacc gccgatccat
    36901 caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat atcaacctgg
    36961 gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga atcattattc
    37021 agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt ggtcaaacct
    37081 ctgccatcgg cattaactta attggtgact atacccatgc tgacttaggc acaggcagca
    37141 ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta acagccactc
    37201 aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat ggcaccagtg
    37261 tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag tacaggtgtt tatatcggtg
    37321 gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac ctgacaatcg
    37381 atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt gtcgatctcg
    37441 gaacaaacag taccattaaa accaatggcg ataatgctca cggcctctgg agctttggcc
    37501 aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat ggcgtcgaag
    37561 ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg cagggcggtg
    37621 gcctcgtcac cagtagttca gacgcgacaa tcaatttttc tggcacggca gcgcaacgaa
    37681 acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg gctgttatca
    37741 acatgcaaaa taccgatatt acggttgatc gtaatggcag tctggcgctg ggtttgtggg
    37801 cgctcagcgg cggtagaata accggagaca gtttggctat caccggcgcg gcaggagcca
    37861 gagggattta tgccatgacc aacagccaga tcgacctcac gagcgatctg gtcattgata
    37921 tgagtacacc cgaccagatg gccatcgcaa cgcaacatga cgatggttat gccgccagcc
    37981 gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt tctttccaaa ggtgggctaa
    38041 tcaatctgga tatgcaccct gggtcggttt ggacaggttc ctccctcagc gataatgtca
    38101 atggcgggaa actggacgtt gcaatgaata acagcgtctg gaacgtaaca agtaattcta
    38161 atctcgacac gctggcgctg agccattcaa ctgtcgattt tgccagccac gggtcaactg
    38221 ccggcacatt taccacatta aacgtagaga acctgagcgg taacagtacc tttattatgc
    38281 gtgctgatgt tgttggcgag ggtaatggcg ttaataatag aggggattta ttgaatatca
    38341 gcgggagtag tgctggtaat cacgtattgg ctatccgcaa ccagggcagc gaggccacaa
    38401 cgggaaatga agttctgaca gtggtaaaaa ccactgacgg cgcggcctcg ttcagcgcgt
    38461 cttctcaggt tgagttgggg ggatatctgt acgatgtgcg taaaaatggc actaactggg
    38521 agctttacgc ttccgggaca gttccggaac cgactcctaa tcctgaaccc acaccagctc
    38581 ccgctcagcc tcccatagtc aaccccgatc ctacgcctga acccgctccc acgcctaaac
    38641 ccaccacgac cgcagatgct ggcggcaatt atctcaatgt cggttactta ttgaactatg
    38701 ttgaaaaccg tacgctgatg caacggatgg gtgacttgcg aaatcagagt aaagacggta
    38761 atatctggtt gcgcagttat gggggaagcc tggactcctt tgccagtggc aaactgagcg
    38821 gctttgacat gggttacagc ggtatccagt ttggtgggga taaacgtctc tctgatgtaa
    38881 tgccgttgta tgtcggtctg tatattgact caacacatgc atcgccggac tatagcggag
    38941 gcgacggtac cgcacgttca gactacatgg gaatgtacgc cagttacatg gcacaaaacg
    39001 gtttttacag cgatctcgtt ataaaagcat cgcgccagaa aaatagtttc cacgtactgg
    39061 acagtcagaa caacggcgtt aacgccaacg gcactgcgaa tggaatgagc atctccctgg
    39121 aagccgggca gaggttcaac ctgtccccta ctggttatgg gttctatata gagccgcaaa
    39181 cccagcttac atacagccac cagaatgaga tggctatgaa ggcgagtaat ggcctcaata
    39241 tacatctgaa tcactacgaa tcgctgctgg ggcgtgccag catgatactg gggtatgaca
    39301 tcaccgcagg caacagccag ctgaatgtct atgtgaagac tggcgctatc cgcgagtttt
    39361 caggggatac cgaatatctg ttgaacgact cccgggagaa gtacagtttc aaaggtaatg
    39421 gctggaataa cggcgtggga gtcagtgcac agtataacaa acagcacaca ttctatctcg
    39481 aagcggatta cacgcagggt aacctctttg atcagaagca agtcaacgga ggatatcgct
    39541 tcagctttta agtgcgggat agacaagccc ttaaacgatg ctgaaaattg ttagcgtgcg
    39601 ccggaatgaa atacagatca ctctgatgta tcagaaatcg tttctgtctt tcatacccgg
    39661 cgcttaaact gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg
    39721 ccgatcccaa gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta
    39781 ataattgcct ctttttcaac ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg
    39841 acatcatctg tcgcctgctc ggtaaacaga tgttcaggta aatcactgac gcgaatgcgc
    39901 ccgttatcac tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcct
    39961 ggccatgcac aagaaactag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag
    40021 aagcgttttt caagactgcg taatttgtta ttcaccagcg ccggaatgct gccacgccgc
    40081 atacgcagag gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta
    40141 aaacgatttt gttccaccag cattgcgagg tccgcggtcg ttgttgcaat cactttgaca
    40201 tcaattggta ttaaacgccg cgcatccagt cgcgtgataa ccccctgctt gataacctgc
    40261 agcaaagcag actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg
    40321 ccgtgtgcca gttccagccg actcagacgg ccattttcat tgtccgtgcg atcgccacca
    40381 ataaattctt ccgccagcgc agcatcacca tataactcac aattgacggc gatataagga
    40441 cctgcagcac gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc
    40501 tcttctccac aaagcaggac aggaaagcta ctgcgcgccg cctggcgacc aaaatgaatc
    40561 aagcggcggg tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct
    40621 aattgactgg tcattaactg ccgcatctgt tccacaggat ggagcaacaa aataaagctg
    40681 gttccctgcg tttctattat cggtttaagg gttatcaccg catcaataaa ctggtgctgg
    40741 ctttcaaagg ttgcttctac gtgtttgagc ggatgtgcca gttttattgc ttgttgcaat
    40801 acggcgggta acgttaagag ttcagtgatt gcccgtccct gacttgccgt cgcgtcaagg
    40861 cgcaagaccc gcgccgcctg ggcattaata aattgcaaat taccctgctc gtcccagcta
    40921 atcacgccat catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt
    40981 tcagcgagca aactgtccgt cagcagtaaa ttaccgacct cgcgggcgat tgccaacgtc
    41041 aacggcaaat cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc
    41101 aatcggccct tgctgtcaaa caacggcgtt gcacaaaagg cccagttcca gagtgcctgt
    41161 ttgaaatgtt gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc
    41221 gcacaagtac caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgcactt
    41281 agctgctgca aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg
    41341 ataaacagcg cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc
    41401 ccgagcgtca gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc
    41461 tgatgtggta cgttccatgt ctcccgtttc atcagcttat tgcatcgctc ccaggaggtt
    41521 gcaattaagg gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc
    41581 ccttatgaca aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca
    41641 atatggaaca ttcattaaag catatgtttc actatgaaac ataatttggg ctaattttct
    41701 tttcccttgc ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac
    41761 atcatccatg ccctaccgta attgctggag caaaataatg aaaaaattga tcaatgatgt
    41821 gcaagacgta ctggacgaac aactggcagg actggcgaaa gcgcatccat cgctgacact
    41881 gcatcaggat ccggtgtatg tcacccgagc tgatgcccct gttgcaggaa aagtcgccct
    41941 gctgtcgggt ggcggcagcg gacacgagcc gatgcactgt ggttatatcg gtcaggggat
    42001 gctttcgggg gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga
    42061 atgctccatg caaattgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg
    42121 cgatattctt aactttgaaa cagcgaccga gttactgcac gatagcggcg taaaagtgac
    42181 cactgtggtc attgatgacg acgttgcggt aaaagacagt ctttataccg ccgggcgacg
    42241 cggcgttgcc aacaccgtat taattgaaaa actcgtaggc gcagcggcgg agcgtggcga
    42301 ctcactggac gcctgtgcgg aactggggcg taagttgaat aatcaaggcc actcaatagg
    42361 tatcgctctc ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta cccttgcgga
    42421 taatgagatg gagtttggcg taggcattca tggtgagcca ggtattgacc gccgcccttt
    42481 ctcttccctt gatcaaaccg tcgatgcaat gttcgacacc ctgctggaac atggctcata
    42541 ccatcgcact ttgcgtttct gggattatca acaaggcagc tggcaggaag aaccacaaac
    42601 caaacaaccg ctccagtctg gcgatcgggt gattgcactg gttaacaatc ttggcgcaac
    42661 tccgctttct gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg
    42721 attgactatc gaacgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt
    42781 ctcaatcacc ttactgaaag ttgatgacga aacgctggct ctctgggacg ccccggtcca
    42841 caccccggcc cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa
    42901 ttgttaactg gctcactcgt tgtggcgata ttttcaacac cgagagcgag tatcttaccg
    42961 ggctggatcg cgagattggc gacgctgacc acgggctaaa tatgaaccga ggctttagca
    43021 aagtggtgga aaaactccct gctatcgcag ataaagatat cggtttcatt ctcaaaaata
    43081 ccggtatgac gctgctttcc agcgtcggtg gtgccagtgg tccgctgttc ggtaccttct
    43141 ttatccgcgc cgcacaggcg acccaggcac ggcaaagcct gacactggaa gagctttatc
    43201 agatgttccg cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata
    43261 aaaccatgtg tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc agcgagcaaa
    43321 atctctctgt tccggtggcg ctcgaagctg ccagtagcat cgccgaatcc gctgcacaaa
    43381 gtacgattac gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg
    43441 gtcaccagga tcccggcgcg acctcggtga tgtttatgat gcaaatgttg gcgttagccg
    43501 caaaagagta aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg
    43561 ggagaaggtg tcggtgaatt agcccgtcag atgttaatga gtgatagttg taaaatcgcc
    43621 attgccgcgg gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg
    43681 gaggccatcg aatctgttgc tgatgccgac catgtgctgg tcatgatgga tatgggtagc
    43741 gcattattga gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta
    43801 cgtttgtgtg ctgcgccgtt ggtcgaaggt acactggcag caacggtcag cgcggcctcg
    43861 ggggcggata tcgacaaagt tatctttgac gctatgcatg cgctggaagc caaacgtgaa
    43921 caactgggtt taccgtcctc cgacactgaa atctctgaca catgtcctcc gtacgatgaa
    43981 gaagcccgtt ctctggcggt ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc
    44041 tcccggctgg tttatacctt atcgacattt aatgccgata tgttgctgga aaaaaacggc
    44101 aaatgcgtca caccagagag tattaaccag attgcgttac tacaagttcg ctataacgat
    44161 acgctgcgcc tgattgcgaa agggccagaa gctgaagagg cactgatcgc tttccgtcac
    44221 ctggctgaag ataactttgg tgaaacggag gaagtcgctc cacctactct gcgtcccgtt
    44281 ctgcctgttt cgggtaaagc cttttattat caaccagttt tatgtacggt acaggcaaaa
    44341 tcaaccctga ccgcggaaga agaacaagat cgattacgcc aggctattga cttcacgtta
    44401 ttagatctga tgacgttaac agcgaaagca gaagccagcg ggcttgacga tattgccgca
    44461 atcttttctg gtcaccatac actgttaggt gatccggaac tgctggcggc ggcaagcgaa
    44521 ctccttcagc atgaacattg cacggcagaa tatgcctggc agcaagttct taaagaactt
    44581 agccagcaat accagcaact ggatgatgaa tatctacaag ctcgctatat tgatgtggac
    44641 gatcttctgc atcgcaccct ggtccacctg acccaaacga aagaagaact cccgcagttt
    44701 aactcgccaa ctattctact ggcggagaac atttatcctt ccacagtact gcaactggat
    44761 ccggcggttg taaaaggtat ctgccttagc gccggaagtc cggtatccca cagcgcccta
    44821 atcgcccgtg aactggggat tggctggatt tgccagcagg gtgagaaact gtatgcgata
    44881 caaccagaag aaacgctaac gctggacgtt aaaacgcaac gtttcaaccg tcagggttaa
    44941 gccaccgttc ggatggcatc gttctgatgt catccggcaa tttacccagc actgatgttt
    45001 tttaactacc tgttatattt tttaccaatt tatgatgatt tttcttcgct aatcgcgaac
    45061 caaatgtcgc tggctggcta ataaacagac gttatttgat cactctattt cgccgttatt
    45121 tatcccttta attccctttt ctaaaatgcc tgaccgttcg cagaatgaga tttcgatcat
    45181 gcagctagtg cgatcctgaa ctaaggtttt ctgatacttg aataccgttt tattcggttt
    45241 cgccaaagga gaatgattga tgaaatcccc cgcaccttct cgcccgcaaa aaatggcgtt
    45301 aattccagcc tgtatctttt tgtgtttcgc tgcgctatcg gtgcaggcag aagaaacacc
    45361 ggtaacacca cagccgcctg atattttatt agggccgctg tttaatgatg tgcaaaacgt
    45421 caaacttttt ccggaccaaa aaacctttgc cgatgccgtg cctaacagcg atccgctgat
    45481 gatccttgct gattatcgga tgcagcaaaa ccagagcgga tttgatctgc gccatttcgt
    45541 taacgtcaat ttcaccctgc cgaaagaagg cgagaaatat gttccgccag aggggcagtc
    45601 actgcgcgaa catattgacg gactttggcc ggtattaacg cgttctaccg aaaacaccga
    45661 aaaatgggat tctctgttac cgctgccgga accttatgtc gtgccgggcg gacgttttcg
    45721 cgaggtatat tactgggaca attacttcac catgttagga cttgccgaaa gtggtcactg
    45781 ggataaaatc gcggatatgg tggccaattt tgctcatgaa ataaacactt acggtcatat
    45841 tcccaacggc aaccgcagtt actatttaag ccgctcgcaa ccgcccttct ttgccctgat
    45901 ggtagagtta ctggcgcagc atgaaggcga tgccgcgttg aagcaatacc tgccgcaaat
    45961 gcaaaaagaa tatgcttact ggatggacgg tgttgaaaac ctgcaagccg gacaacagga
    46021 aaaacgcgtt gtaaaacttc aggatagtac ccttctcaac cgctactggg acgatcgcga
    46081 tacgccacga ccagagtcat gggtggaaga tattgccacc gccaaaagca atccgaatcg
    46141 acctgccact gaaatttacc gcgacctgcg ctctgccgct gcgtctggct gggatttcag
    46201 ctcgcgctgg atggacaacc cgcagcagtt aaatacctta cgcaccacca gcatcgtacc
    46261 ggtcgatctg aacagcctga tgtttaaaat ggaaaaaatc ctcgcccgcg ccagcaaagc
    46321 tgcaggagat aacgcgatgg caaaccagta cgaaacgctg gcgaatgccc gtcaaaaagg
    46381 gatcgaaaaa tacctgtgga acgatcaaca aggctggtat gccgattacg acctgaaaag
    46441 tcataaagtg cgcaatcagt taaccgcggc cgccctgttc ccgctgtacg tcaatgcggc
    46501 agcgaaagat cgcgccaaca aaatggcgac ggcgacgaaa acacatctgc tgcaacccgg
    46561 cggcctgaac accacgtcgg tgaaaagtgg gcaacaatgg gatgcgccaa atggctgggc
    46621 accgttacag tgggtcgcga cagaaggatt acaaaactac gggcaaaaag aggtggcgat
    46681 ggacattagc tggcacttcc tgaccaatgt tcagcacacc tatgaccggg agaaaaagct
    46741 ggtggaaaaa tatgatgtca gcaccaccgg aacggggggc ggcggtggcg aatatccatt
    46801 acaggatggc tttggctgga ccaatggcgt gacgctgaaa atgctggatt tgatctgccc
    46861 gaaagagcaa ccgtgtgaca atgttccggc gacgcatccg accgttaagt cagcaacgac
    46921 gcaaccctca accaaagagg cacaacccac accttaacca gcgcttactc cgacaacgtc
    46981 attcggcttc tccggatggc gttgtcggca gataacacca tatctatggt tttttctaca
    47041 cagacggttt ttcaagtcct ggcgggaagt attgcgcaaa tgtttgccat acgtggttgc
    47101 cgaagatagc gtcatttcta cgggtaagtg tttcactttc atcacccggc gatttacgcc
    47161 gacgttggct atataaagca tcaattgcta c
//
LOCUS       DAJTUP010000022        45648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTUP010000022 DAJTUP010000000
VERSION     DAJTUP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.022"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..223
                     /locus_tag="OO468_001257"
                     /pseudo
     CDS             1..223
                     /locus_tag="OO468_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005094514.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            422..2110
                     /locus_tag="OO468_001258"
     CDS             422..2110
                     /locus_tag="OO468_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6258443.1"
                     /translation="MQSPLPYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNL
                     SDSKFISSFKSWFESGKIKNIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGE
                     FITYYENGAIESKRHFNNDKIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGK
                     LMQENFYQQSVLIGNDTSYYQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKD
                     GKQISVQTWYENGQKEEEKHFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEW
                     YENGIPESLYPYKNGKTDGIAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKE
                     TLYVNGIRQGVEKEFNDRTGKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLS
                     SLDNPTQIKDNATTGDSSAQFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATA
                     YKGNGIPANNEKYLAYLQQAATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAA
                     QGNFAAHFYLGRMYQNGDGVERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2163..3894
                     /locus_tag="OO468_001259"
                     /pseudo
     CDS             2163..3894
                     /locus_tag="OO468_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3954..4445)
                     /gene="yajQ"
                     /locus_tag="OO468_001260"
     CDS             complement(3954..4445)
                     /gene="yajQ"
                     /locus_tag="OO468_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR6258444.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4613..5524
                     /gene="panE"
                     /locus_tag="OO468_001261"
     CDS             4613..5524
                     /gene="panE"
                     /locus_tag="OO468_001261"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR6258445.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5487..6077
                     /gene="yajL"
                     /locus_tag="OO468_001262"
     CDS             5487..6077
                     /gene="yajL"
                     /locus_tag="OO468_001262"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR6258446.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6131..7579)
                     /gene="thiI"
                     /locus_tag="OO468_001263"
     CDS             complement(6131..7579)
                     /gene="thiI"
                     /locus_tag="OO468_001263"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR6258447.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7785..8027
                     /gene="xseB"
                     /locus_tag="OO468_001264"
     CDS             7785..8027
                     /gene="xseB"
                     /locus_tag="OO468_001264"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR6258448.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8027..8926
                     /gene="ispA"
                     /locus_tag="OO468_001265"
     CDS             8027..8926
                     /gene="ispA"
                     /locus_tag="OO468_001265"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR6258449.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            8951..10813
                     /gene="dxs"
                     /locus_tag="OO468_001266"
     CDS             8951..10813
                     /gene="dxs"
                     /locus_tag="OO468_001266"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR6258450.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            10868..11785
                     /gene="yajO"
                     /locus_tag="OO468_001267"
     CDS             10868..11785
                     /gene="yajO"
                     /locus_tag="OO468_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR6258451.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(11896..12414)
                     /gene="pgpA"
                     /locus_tag="OO468_001268"
     CDS             complement(11896..12414)
                     /gene="pgpA"
                     /locus_tag="OO468_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR6258452.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12392..13369)
                     /gene="thiL"
                     /locus_tag="OO468_001269"
     CDS             complement(12392..13369)
                     /gene="thiL"
                     /locus_tag="OO468_001269"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR6258453.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13447..13866)
                     /gene="nusB"
                     /locus_tag="OO468_001270"
     CDS             complement(13447..13866)
                     /gene="nusB"
                     /locus_tag="OO468_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR6258454.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(13886..14356)
                     /gene="ribE"
                     /locus_tag="OO468_001271"
     CDS             complement(13886..14356)
                     /gene="ribE"
                     /locus_tag="OO468_001271"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR6258455.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTVLEMINVLKAIKA"
     gene            complement(14445..15548)
                     /gene="ribD"
                     /locus_tag="OO468_001272"
     CDS             complement(14445..15548)
                     /gene="ribD"
                     /locus_tag="OO468_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR6258456.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15552..16001)
                     /gene="nrdR"
                     /locus_tag="OO468_001273"
     CDS             complement(15552..16001)
                     /gene="nrdR"
                     /locus_tag="OO468_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR6258457.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16152..16691
                     /locus_tag="OO468_001274"
     CDS             16152..16691
                     /locus_tag="OO468_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6258458.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            16989..17873
                     /gene="tsx"
                     /locus_tag="OO468_001275"
     CDS             16989..17873
                     /gene="tsx"
                     /locus_tag="OO468_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR6258459.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18050..18397)
                     /locus_tag="OO468_001276"
     CDS             complement(18050..18397)
                     /locus_tag="OO468_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR6258460.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18526..19497)
                     /gene="secF"
                     /locus_tag="OO468_001277"
     CDS             complement(18526..19497)
                     /gene="secF"
                     /locus_tag="OO468_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR6258461.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19508..21355)
                     /gene="secD"
                     /locus_tag="OO468_001278"
     CDS             complement(19508..21355)
                     /gene="secD"
                     /locus_tag="OO468_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR6258462.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21383..21715)
                     /gene="yajC"
                     /locus_tag="OO468_001279"
     CDS             complement(21383..21715)
                     /gene="yajC"
                     /locus_tag="OO468_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR6258463.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21738..22865)
                     /gene="tgt"
                     /locus_tag="OO468_001280"
     CDS             complement(21738..22865)
                     /gene="tgt"
                     /locus_tag="OO468_001280"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR6258464.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(22920..23990)
                     /gene="queA"
                     /locus_tag="OO468_001281"
     CDS             complement(22920..23990)
                     /gene="queA"
                     /locus_tag="OO468_001281"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR6258465.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24083..24656
                     /gene="acpH"
                     /locus_tag="OO468_001282"
                     /pseudo
     CDS             24083..24656
                     /gene="acpH"
                     /locus_tag="OO468_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24661..26478)
                     /gene="malZ"
                     /locus_tag="OO468_001283"
     CDS             complement(24661..26478)
                     /gene="malZ"
                     /locus_tag="OO468_001283"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR6258466.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26634..28007)
                     /gene="proY"
                     /locus_tag="OO468_001284"
     CDS             complement(26634..28007)
                     /gene="proY"
                     /locus_tag="OO468_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR6258467.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28083..29402)
                     /gene="brnQ"
                     /locus_tag="OO468_001285"
     CDS             complement(28083..29402)
                     /gene="brnQ"
                     /locus_tag="OO468_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR6258468.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29809..31104)
                     /gene="phoR"
                     /locus_tag="OO468_001286"
     CDS             complement(29809..31104)
                     /gene="phoR"
                     /locus_tag="OO468_001286"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR6258469.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31162..31851)
                     /gene="phoB"
                     /locus_tag="OO468_001287"
     CDS             complement(31162..31851)
                     /gene="phoB"
                     /locus_tag="OO468_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR6258470.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32041..33243
                     /gene="sbcD"
                     /locus_tag="OO468_001288"
     CDS             32041..33243
                     /gene="sbcD"
                     /locus_tag="OO468_001288"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR6258471.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33240..36383
                     /gene="sbcC"
                     /locus_tag="OO468_001289"
     CDS             33240..36383
                     /gene="sbcC"
                     /locus_tag="OO468_001289"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR6258472.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36509..37690
                     /gene="araJ"
                     /locus_tag="OO468_001290"
     CDS             36509..37690
                     /gene="araJ"
                     /locus_tag="OO468_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR6258473.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37833..38741)
                     /gene="mak"
                     /locus_tag="OO468_001291"
     CDS             complement(37833..38741)
                     /gene="mak"
                     /locus_tag="OO468_001291"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR6258474.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            38866..39777
                     /gene="rdgC"
                     /locus_tag="OO468_001292"
     CDS             38866..39777
                     /gene="rdgC"
                     /locus_tag="OO468_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR6258475.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39855..40310)
                     /locus_tag="OO468_001293"
     CDS             complement(39855..40310)
                     /locus_tag="OO468_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258476.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40307..40642)
                     /locus_tag="OO468_001294"
                     /pseudo
     CDS             complement(40307..40642)
                     /locus_tag="OO468_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40797..41081)
                     /gene="ppnP"
                     /locus_tag="OO468_001295"
     CDS             complement(40797..41081)
                     /gene="ppnP"
                     /locus_tag="OO468_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR6258477.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41153..41830)
                     /locus_tag="OO468_001296"
     CDS             complement(41153..41830)
                     /locus_tag="OO468_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR6258478.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42088..42279)
                     /gene="yaiA"
                     /locus_tag="OO468_001297"
     CDS             complement(42088..42279)
                     /gene="yaiA"
                     /locus_tag="OO468_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR6258479.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42329..42853)
                     /gene="aroL"
                     /locus_tag="OO468_001298"
     CDS             complement(42329..42853)
                     /gene="aroL"
                     /locus_tag="OO468_001298"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR6258480.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43036..43494)
                     /locus_tag="OO468_001299"
     CDS             complement(43036..43494)
                     /locus_tag="OO468_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR6258481.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43614..44423
                     /gene="proC"
                     /locus_tag="OO468_001300"
     CDS             43614..44423
                     /gene="proC"
                     /locus_tag="OO468_001300"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR6258482.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44440..45555)
                     /gene="adrA"
                     /locus_tag="OO468_001301"
                     /gene_synonym="dgcC"
     CDS             complement(44440..45555)
                     /gene="adrA"
                     /locus_tag="OO468_001301"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR6258483.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11852 a        11922 c        11184 g        10690 t
ORIGIN      
        1 cagttcatat gcaaaaagcg aatcccctcc cgcaagcact ataactttca ttgcacaaat
       61 cgatcatgtt gagctaaata aaacagcatt atctcatgat atgaaactcg ttcttgccga
      121 tcgtttttat ttcaaaacca gtaatccttg cgaaacaaat tcaatttctg ctcgaccaga
      181 atcaatggat attactgaca aagcctatgc cgacaaatta aaaaaattag aaactccttt
      241 tcttgatgat cgctatttct ttatgaccat caatcagtgt gacggtaatg gcactcccat
      301 gctgacagac ataaaagtat gtacaacaga attatgtggt gctgaatata caaagaaaat
      361 gtctactgta tggctcgatg aaactttaaa accaactgct aaacgtctgg caatttattt
      421 tattcaatct cctttaccct atgacgagga gaaaaaacaa tggaaagtca ccggatggta
      481 tatctcatca actgataatc agtcttcaac taaacagatt gcatttgaag gatatacaga
      541 cacagataac ctctccgatt ctaaatttat ttcttcattt aaatcctggt ttgaaagtgg
      601 aaagataaaa aacattgcta actataatcc aaaaggagaa agagatggaa aatacgaaag
      661 ttattacaat agcaatggta acgtctatga atctctaagt ttttctaatg gtcttatcaa
      721 cggtgagttt attacatatt atgaaaatgg ggccattgaa agtaaacgtc attttaataa
      781 cgacaaaatt aaagatggtg aatgccctca tttttatgaa gatgggaaaa tcaaacagca
      841 acatagctat ctgaatagta aactcgatgg tcctgcatat gaatattatc ctgatggcaa
      901 actcatgcag gaaaatttct atcagcaaag tgtgttgatt ggaaacgata ccagttacta
      961 ccaatccgga aaaataagct ctatacacaa tagaaattcc cgtggtcagt atgatggagc
     1021 taacgaacgt tatagtgaag agggtaagtt actgtctaaa tccgtctata aagacggaaa
     1081 acaaatttct gttcaaacct ggtatgaaaa tgggcagaaa gaagaggaga aacattttga
     1141 tgagcaaggc caattaaatg gtctggttaa gcaatggtat aaaaatggta atcttgcaaa
     1201 atcacaaaac tataaacatg acatactcga tggtaacagt gaagagtggt acgaaaatgg
     1261 catccctgaa agtttgtacc cttataagaa tggaaaaacc gacggcattg ccaaatcatg
     1321 gaataagtat ggtaaattaa cctattcaat cgaatacaaa aatggcgtag agaatggggc
     1381 atatcgtaac tggagcaaaa acacaggtaa actaaccaaa gaaaccctgt acgtcaatgg
     1441 tatcagacag ggagtggaaa aagagtttaa cgatcgcaca ggtaaactat taacctccac
     1501 gcaatatgtt aataataaaa gacatggtac ggaggaaact tatgatcaaa acggaataaa
     1561 atatattaca tgctatcaaa atgatcagaa attatcgtct ttagacaatc caacacaaat
     1621 aaaagataat gcaacaacag gcgattccag tgctcaattt gcattaggga aatacgaatt
     1681 catctgtgca aatatagatg aagggattaa atggttaacc aaatctgcag agcaaaaaaa
     1741 cactgatgcg atatactttt tagctacagc ctacaaaggc aatggtatac ctgcaaacaa
     1801 tgaaaaatac ctagcatatc tgcaacaagc tgcaacactg ggcagtagta atgcccaggc
     1861 ggaaattggc tatttatatc tcatcggaaa agaactacca cagaatcttc ctgatgctgg
     1921 tgtctggttc aaaaaagcag ctgcacaagg taattttgcc gcacatttct atctgggaag
     1981 gatgtatcag aatggtgacg gcgttgagag aaatatggaa aaagccaggt ttcatctatc
     2041 taatgcggca gaaggtggaa tagaacctgc gcttaaagca ttaaatgaac ttgaacatca
     2101 aacaaaataa agcgctcagg gaccacgtaa ggttcatcaa acagaaaagg atatagtttg
     2161 cgatgaaagc gaaatccact ttgttattaa tgattttatc tgttttttat tctcatcagg
     2221 ttttatccag cgaacccact cagccggatg tttattctgt cgtcgagaaa aaactaaact
     2281 ctgctttacc tctggaagaa aataccacct tcagatcgca ggctgaatgg tttaatttgc
     2341 aatatgaatt attagtatct ggctaccctg aacgtgctta caatttactg agcaaacttg
     2401 agacagaatc gaaaaacgct aaatattatc tggatttaac tgcctccaga gtcgctgaag
     2461 aagaagcacc agagacagcc ttaacttttt tatcaaaaat tgggttaaat aaaccggcta
     2521 ctcttacatc ctttgagagc tatatcaata gctggataaa aagcaaacaa cccgaaaagg
     2581 ctatgcagct tctcagcctc gatgagaatg ccctgaatta ttatttaccg actgtgttag
     2641 tcgcatatca gatcgttcct gataaggcaa taagtattta taattctgta tatggagata
     2701 atgttgttat accctatcag caagtcacca tgttacttac agttgcggag ggttatcaaa
     2761 caaaaggcga tataaaaaac gccattcatt atgccgataa ggcgctgaat atgtttgata
     2821 aagctattgc tgtagatagc agtcgtgaat cccattatag tgatgagtat ttgaaactca
     2881 tgaatatcta cgccgccaat ggcaacaaag aaaaagcggt ggttttatct caacgtctac
     2941 acaaagccat taccgaatct caatcgaatt acttaagtac tctggaagga attttattgt
     3001 tttatagaaa taatgatatg cagcaagact atcaaaacag cctttcaaat tacatagtat
     3061 ttatcgataa aatttttgcc ttttccccat caacaagaag tgaattaagc ctcattaatt
     3121 tattaaattc attaaatgag gtggaattga tgagaaaacg tctggcgttt ctgatgacag
     3181 caccagaata cgcttgttat aatagtcagt attgttatga agacaaagtc agcgcattaa
     3241 aaatcctgta tcgtcatcat gacaatgagc taatggacaa atatttcacg actcttctta
     3301 aagagatcag taatcaacca ttagaagact gggatataat aataagtaat actggtgaaa
     3361 aatttagtga agtgggtcta accaaatatg ctcaacatct cgcatctttc gctgaagaat
     3421 tttatataaa agagcaaaaa tcacattctg aaagtgaaat gagaggacta ttttcatcac
     3481 ttgccgaatt atatagtttt ggtaatgata ctgccagcgc aaaccgagtg ttccatcagc
     3541 atgtccctgc cttcaataca gatcatatga ttcaatattt tataaatgcc aaagaatgga
     3601 acaatgccag ggaattattg ataaaagagg aaatgttggg tatgcataat ctgatattgt
     3661 tggagaatat ttgtatgcaa aaaaaatgca gaatgcatgg cacacattac ctttacgctt
     3721 aataaattaa caacacaacc agcaataaca aaaatagata gcgtgggtaa tgagcaactt
     3781 tatcagattg gcaaaatcta ccataagttg gaaattaaac ctgagccaga gcaacaggtt
     3841 ttaattcagt cgctttatga ccgggcatca ggaagcgcct ctgcaactca ataaatgcat
     3901 ggtcaaaagg gccaccacgg tggccctgac aatcagtagc tatatccaga aagttaatcg
     3961 cggaagtttt tgaactggaa cggctgaccg agatcgccac cgcgcaccat cgccatgaca
     4021 gcctgcaaat catcacgaga tttgccagtc acgcggatct catcgccctg aatttgcgcc
     4081 tgcactttca gtttgctgtc tttgatcatc ttgacgattt ttttctgagt ggcgctctca
     4141 atgccctgtt tcagtttcgc ttccacaaac caggttttac cgctatgaac gatattttcc
     4201 ggtacatcca gtgaactgcc ttcaatgccg cgcttcagca gcttggcacg cagaatatcc
     4261 agcaactgat tgacctggaa gtcggactcg ctcaacactt tgatggtttt gctggcgtcg
     4321 ttcagctcaa atgaggcttc aacgttacgg aagtcaaaac gggactccac ttcgcgactc
     4381 gcgttatcga ccgcgttacg tgcttcctga agatcaactt cagagacaat atcgaaagat
     4441 ggcatctttt cctctccctt catttttgat gcgaagcata atacctgcaa agttaaataa
     4501 ccaacagtcc tgcaccagga cgtatccctt aattgtgcat agtcgcaaca gcatgcacaa
     4561 ttaagggata gccttattga acacccggag tggttgcggg tgaggaggaa caatgaaaat
     4621 taccgtattg ggatgcggtg ccttagggca attatggctt acagcacttt gtaaacaggg
     4681 tcatgaagtt cagggctggc tgcgtgtacc gcaaccttat tgtagtgtga atctggttga
     4741 gacagatggt tcgatattta acgaatcgct gaccgccaac gatcccgatt ttctcgccac
     4801 cagcgatctg ctcctggtga cgctgaaagc atggcaggtt tccgatgccg tcaaaagcct
     4861 cgcatccaca ctgcctgtaa ctacgccaat actgttaatt cacaacggca tgggcaccat
     4921 cgaagaattg caaaacattc agcagccatt actgatgggc accaccaccc atgccgcccg
     4981 ccgcgacggc aatgtcatta ttcatgtggc aaacggaatc acgcatatcg gtccggcacg
     5041 gcaacaggat ggcgattaca gttatctggc ggatattttg caaaccgtgt tgcctgacgt
     5101 tgcctggcat aacaatattc gcgccgagct gtggcgcaag ctggccgtca actgcgtgat
     5161 taatccactg actgccatct ggaattgccc gaacggtgaa ttacgtcatc atccgcaaga
     5221 aattatgcaa atatgcgaag aagtcgcggc agtgatcgaa cgcgaagggc atcatacttc
     5281 agcggaagat ttgcgtgatt acgtgatgca ggtgattgat gccacagcag aaaatatctc
     5341 gtcgatgttg caggatatcc gcgcgctgcg ccacaccgaa atcgactata tcaatggttt
     5401 tctcttacgc cgcgcccgcg cgcatgggat tgccgtaccg gaaaacaccc gcctgtttga
     5461 aatggtaaaa agaaaggaga gtgaatatga gcgcatcggc actggtttgc ctcgcccctg
     5521 gtagtgaaga gactgaagcc gtcaccacga tcaacctctt ggttcgcggc ggtatcaaag
     5581 tcaccactgc cagcgtcgac agcgatggca acctggcaat tacctgctcg cgcggcgtga
     5641 agctgctggc ggatgcgccg ctggtcgaag tggctgatgg cgaatatgac gtgatcgtgc
     5701 tgcctggtgg cattaaaggc acggagtgtt ttcgcgatag cactctgctg gttgaaaccg
     5761 ttaaacagtt ccaccgttcc gggcgtatcg tcgcggctat ttgcgccgcg ccagccaccg
     5821 tgctggtgcc gcacgatatc tttccgattg gcaatatgac cggcttcccg acgctgaaag
     5881 acaaaattcc cgctgaacaa tggcaggaca agcgcgtcgt ctgggatgca cgggtaaaat
     5941 tgctgaccag ccaggggccg ggtacagcta tcgactttgg tctgaaaatt atcgacctgc
     6001 tggttgggcg cgagaaagcc catgaagtgg catcacaact ggtgatggcg gcagggattt
     6061 ataattatta cgagtagtgt cggatgcggc aaacgtcgca tctgaccaga tgcgacgtaa
     6121 aaactacaaa ttacgggcga tataccttca cattgttaaa gccttgctcg cgcagataga
     6181 gcgcctgcag acggctcatc accccgcgct cacaccacag cagccaggtt ttgttctggt
     6241 cgagatcgcc aaatttggtg ctcagtttat agaacggcag agaaaccaca tcaatccctt
     6301 cgactttcag tggcttatct tcctgttcat cgacagaacg gatatcgaga atcacgtcgt
     6361 tcgggccgaa gccgttgacg gtttccactt ccaccacttc ctgctcggtc tgctgggcga
     6421 tttcgcggat atcaacgtta ttcgcttcct caaccacttt atcgagaata ctgaagtcga
     6481 acttctcttc ttccgcttca atcttcgatt taaccgcttt caccgtcggg cttttggaga
     6541 tcacgccaca atattccggc atcgtgcgag caaagtcttc ggtgccaatc tggcgagcca
     6601 ggttgatgat gtgctctttg tcgtaagaaa tcagcggacg caggatcagc gtgtcagaga
     6661 cgttatcaat caggcgcagg ttggtcagcg tctgactgga cacctggccg agcgcttcgc
     6721 cggtgaccag cgcctgtacg ccgtaacgtt cagccacttt agatgcggca cgcaccatca
     6781 tacgtttgag gataacgccc atctgaccgt cgtcgatttt ctcgagaatt tccccgacaa
     6841 ccggttcgaa attaatggcg acaaaacgca cgcggtggga gctgccaaag cggttccaca
     6901 gataatgcgc cacctgacgc acgccaattt catgcgctgc gccgccgagg ttaaagaagc
     6961 agtaatgcac gcggcagccg cgacgcatca acatataact ggaaacaccg gagtcgaaac
     7021 caccggaaat gagcgacagc acatcttcct gggtgccaat cgggaaacca ccaatacctt
     7081 cgtagcgacc tttaatcagc aggaggcgat cgtcttccac ttccagatga acagttacat
     7141 ccgggttagt cagcttcacg cgcgcggatt caatatgctg atttaaaccg ccgccgacgt
     7201 aacgttccac atcaatcgag ctaaaatcat gtttgccacg gcgcttcacg cgtacgcaga
     7261 aggttttgcc ttccagctga tcgcgatact gaaccaacgc tttctcaaaa atatcgtgca
     7321 tgtcggtaaa cggcacgtct tcgacttcga gaatatggtg gatacccgga atacgggtca
     7381 gggcgtcgcg aatagtcaga cgctggtttt catctttcgc gcgaacttcg atgttatccc
     7441 agtggcggac gacagcgagc gtctcatcat agtgctttaa aacgttacga atgttcccgg
     7501 taaggatttt tataaagcgc aagcgcacag attggctttt gatggtgatt tccgggaaca
     7561 atttaatgat aaacttcatg gcggcaatgg ttcgttggca agccttaagc gacttgtata
     7621 gggaaaaata cagcagccca cacctgcggc tgcatccagg cgcggaagta taccactaac
     7681 atcgctttgc tgcgcacatc accttaccat tgcgcgttat ttgctatttg ccctgagtcc
     7741 gttaccatga cggggcgaaa aatattgaga gtcagacatt cattatgccg aagaaaaatg
     7801 aggcgcccgc cagctttgaa aaggcgctga gcgagctgga acagattgta acccgtctgg
     7861 aaagtggcga cctgccgctg gaagaggcgc tgaacgagtt cgaacgcggc gtgcagctgg
     7921 cacgtcaggg gcaggccaaa ttacaacaag ccgaacagcg cgtacaaatt ctgctgtctg
     7981 acaatgaaga cgcctctcta acccctttta caccggacaa tgagtaatgg actttccgca
     8041 gcaactcgaa gcctgcgtta agcaggccaa ccaggcgctg agccgtttta tcgccccact
     8101 gccctttcag aacactcccg tggtcgaaac catgcagtat ggcgcattat taggtggtaa
     8161 gcgcctgcga cctttcctgg tttatgccac cggtcatatg tttggcgtta gcacaaacac
     8221 gctggacgca cccgctgctg ccgtagagtg tatccacgct tactcattaa ttcatgatga
     8281 tttaccggcg atggatgatg acgatctgcg tcgcggtttg ccgacctgcc atgtgaagtt
     8341 tggcgaagca aacgcgattc tcgctggcga cgctttacaa acgctggcgt tctcgattct
     8401 aagcgatgcc aatatgccgg aagtgtcgga tcgcgacaga atttcgatga tttctgaact
     8461 ggcgagcgcc agcggtattg ccggaatgtg cggtggtcag gcattagatt tagacgcgga
     8521 aggcaaacac gtacctctgg acgcgcttga gcgtattcat cgtcataaaa ccggcgcatt
     8581 gattcgcgcc gccgttcgcc ttggtgcatt aagcgccgga gataaagggc gtcgtgctct
     8641 gccagtactc gacaagtacg cagagagcat cggccttgcc ttccaggttc aggatgacat
     8701 cctggatgtg gtaggagata ctgcaacgtt gggaaaacgc cagggtgccg accagcaact
     8761 tggtaaaagt acctaccctg cacttctggg tcttgagcaa gcccggaaga aagcccggga
     8821 tctgatcgac gatgcccgtc aggcgctgaa acaactggct gaacagtcac tcgatacctc
     8881 ggcactggaa gcgctagcgg actacatcat ccagcgtaat aaataaacaa taagtattaa
     8941 taggcccctg atgagttttg atattgccaa atacccgacc ctggcactgg tcgactccac
     9001 ccaggagtta cgactgttgc cgaaagagag tttaccgaaa ctctgcgacg aactgcgccg
     9061 ctatttactc gacagcgtga gccgttccag cgggcacttc gcctccgggc tgggcacggt
     9121 cgaactgacc gtggcgctgc actatgtcta caacacccca tttgaccaat tgatttggga
     9181 tgtgggacat caggcttatc cgcataaaat tttgaccgga cgccgcgaca aaatcggcac
     9241 catccgtcag aaaggcggtc tgcacccgtt cccgtggcgc ggcgaaagcg aatatgacgt
     9301 attaagcgtc gggcattcat caacctccat cagtgccgga attggtattg cggttgctgc
     9361 cgagaaagaa ggcaaaaatc gccgcaccgt ctgtgtgatt ggcgacggcg cgattaccgc
     9421 tggcatggcg tttgaagcga tgaatcacgc gggcgatatt cgtcctgata tgctggtggt
     9481 cctcaacgac aatgaaatgt cgatttccga aaatgttggc gcgcttaaca accatctggc
     9541 tcagctgctt tccggtaagc tttactcttc actgcgcgaa ggcgggaaaa aagttttctc
     9601 tggcgtgccg cccattaaag agctgctcaa acgtaccgaa gaacatatta aaggcatggt
     9661 ggtgcctggc acgttgtttg aagagctggg atttaactac atcggcccgg tggacggtca
     9721 cgatgtgctg gggcttatca ccacgctaaa gaacatgcgc gacctgaaag gcccgcagtt
     9781 cctgcatatc atgaccaaaa aaggtcgtgg ttatgaaccg gcagaaaaag acccgatcac
     9841 tttccacgcc gtgcctaaat ttgatccctc cagcggttgt ttgccgaaaa gtagcggcgg
     9901 tttgccgagc tattcaaaaa tctttggcga ctggttgtgc gaaacggcag cgaaagacaa
     9961 caagctgatg gcgattactc cggcgatgcg tgaaggttcc ggcatggtcg agttttcacg
    10021 taaattcccg gatcgttact tcgacgtggc tattgccgag caacacgcag tgacctttgc
    10081 cgccggtctg gcgattggtg gatacaaacc cattgtcgcg atctactcca ccttcctgca
    10141 acgcgcctat gatcaggtgc tgcatgacgt ggcaattcaa aagctaccgg tcctgttcgc
    10201 cattgaccgc gcgggcattg ttggtgctga cggtcaaact caccagggcg cttttgatct
    10261 ctcttacctg cgctgcatac cagaaatggt cattatgacc ccgagcgatg aaaacgaatg
    10321 tcgccagatg ctctataccg gctatcacta taacgatggc ccgtccgcgg tgcgctaccc
    10381 gcgcggcaac gcggttggcg tggaactgac gccgctggaa aaactaccaa ttggcaaagg
    10441 cattgtgaag cgtcgtggcg agaaactggt gatccttaac tttggtacgc tgatgccaga
    10501 aacggccaaa gtcgccgaat cgctgaacgc tacgctggtc gatatgcgtt ttgtgaaacc
    10561 acttgatgaa gcgttaattc tggaaatggc cgccagccat gaagcgctgg tcaccgtaga
    10621 agaaaacgcc attatgggcg gcgcaggtag cggagtgaac gaagtgctga tggcccatcg
    10681 taaaccagta cccgtgctga acattggcct gccggacttc tttattccgc aaggaactca
    10741 ggaagaaatg cgcgccgaac tcggcctcga tgccgccggt atggaagcca aaatcaaggc
    10801 ctggctggca taatccctac tccactcctg ctatgcttaa gaaattattc atggcaggag
    10861 tggcagcatg caatacaacc ccttaggaaa aaccgacctt cgcgtttccc gactttgcct
    10921 cggctgtatg acctttggcg agccagatcg cggtaatcac gcatggacac tgccggaaga
    10981 gagcagccgt cccataatta aacgcgcgct ggaaggtggc ataaatttct ttgataccgc
    11041 caatagctat tccgatggta gcagcgaaga gatcgttggt cgcgcactgc gggatttcgc
    11101 ccgtcgtgaa gacgtagtcg ttgccaccaa agtgttccat cgcgttggtg atttaccgga
    11161 aggattatcc cgtgcacaaa ttttgcgctc tatcaacgac agcctgcgac gtctcggcat
    11221 ggattatgtc gatatcctgc aaattcatcg ctgggattac aacacgccga tcgaagagac
    11281 gctggaagcc ctgaacgacg tggtaaaagc cgggaaagcg cgttatatcg gcgcgtcatc
    11341 catgcacgct tcgcagtttg ctcaggcgct ggaactacaa aaacagcact gctgggcgca
    11401 gtttgtcagt atgcaggatc actataatct gatttatcgc gaagaagagc gcgagatgct
    11461 gcctctgtgt tatcaggagg gcgtggcggt gattccgtgg agcccgctgg cgcgggggcg
    11521 actgacgcgt ccatggggag aaactaccgc acgactggtg tctgatgagg tggggaaaaa
    11581 tctctataaa gaaagcgatg aaaatgacgc gcagatcgca gagcggttaa cgggcgtcag
    11641 tgaagaactc ggtgcaacac gagcacaagt tgcgctggcc tggttgttaa gtaaaccggg
    11701 cattgccgca ccgattatcg gaacttcgcg ggaagaacag cttgatgagc tgctgaacgc
    11761 ggtggatatc actttgaagc cataacagat tgccgagctg gaaacgccgt ataaaccgca
    11821 tgcggtcgta ggatttaaat aagagtccag gcctgatgag acgtgacaag cgtcacatca
    11881 ggcatcggtg cacaactacg acagaatacc cagcggccag tgatgaccga taaagtacag
    11941 gatgcctgcg gaaatcaccc cggcgacaat atcgtcgatc atgatcccca tgccgccatg
    12001 tacattgcga tcaaaccagc ggatcggcca cggcttccac atatccagaa tacggaaaat
    12061 cacaaacccg gcggcaaccc actgccagtc attggtcggc agcgccatga gcgtgatcca
    12121 cataccaata aattcgtccc agacaatgct gccgtgatcg tgcacgccca tatctttcgc
    12181 cgtttgatga caaagataga cgccgataca gatccccaac atcaccacca gcgagtagag
    12241 ctgccagggt aaaaaggtca tcagatacca gaaagggatc gccgccagcg atcccatcga
    12301 gccaggaacg attgggctta atccacttcc gaatccgaca gcaagcagat gccacggatt
    12361 actcatcttc aggcgacttt tcgcgacatc tttatggcgt gacaaaatgg tcatatcctt
    12421 tccagtcaaa cgtgacaggt tcgccgtcac gaataaaaca aagcccttcg atatcggcgg
    12481 tcatttgccc gatacaggta aacggtacgc caaggtgtcc gagagcaaca tccagcgcgc
    12541 cacggttcag ttccggcacc gtgaaacaca actcgtaatc ttcaccgcca gagagcgccc
    12601 agcgcagcgc ctgttccggt tcaacatggc gagaaagcgc atcagaaaac ggcagcaatg
    12661 ccaggtcaat acgtgcgccg cagtcgctgg ctttcacgat atgcccgaga tcggaaatca
    12721 gaccgtcaga gagatcgata gctgaatttg ccagatcgcg cagtgcctgc ccctgtaaaa
    12781 tacgcggcga tggacggaga tgacgtttga tcaagtagtc agcatcttta gcatcggcaa
    12841 cctgcaaacg gttttgcaaa atcgccagcc cggcggcgct atcgcccggt gtaccggtca
    12901 catagatcca gtcacccggt ttcgccccag agcgcgttaa ggctcgtccc atcggaacaa
    12961 agccgtggat acccaacgtc attgataatg gcccacgcgt ggtatcgccg ccaatgagtt
    13021 gcatatcgta ataattgaga agatcaaaca aactgtcgct gaaggactca agccacgctt
    13081 cgtctacgtc cggtaaggtt aatgccagcg tcagccaggc cggatcggcc cccatcgctg
    13141 ccagatcgct taggttcacc gccagtgctt tataagccag atcagcagga tcgatatcag
    13201 ggaggaaatg gttgcccgcc accagcgtat cagtgctgat cgccagggtc tgtttctcgg
    13261 ggatattgag aagagcgcaa tcgtcgccga tgcccagttc gacatcaaga cgagaacttc
    13321 ttacacggtc aaaataacgg gcaatcaggg agaactcgcc acatgccata cgttatgcct
    13381 cagcaggtaa aaaagaaaag gccggcgatc atggaacagt cttccgtgaa tctaccggcc
    13441 tggatattac tttttgttag ggcgaatcac aggtgctgct ttatcgagta cgccgttgac
    13501 gaacttatgg ctgtcttctg cgccgaacga tttcgccagt tcgatcgctt cgttaatggc
    13561 cactttgtat ggcacatcac tacgtttaga cagttcgtac agcgcaatgc gcagtactgc
    13621 tttttctacc tggcccagtt cttccagcag gcgggacagg tagggcttca tcagtccgtc
    13681 gaggtatgcg gtgttagtcg ccaccccggc cagcagctca cggaagtaca ggacgtcaac
    13741 gtcttttaca tcctgttcag ccaggaactg gtattcaaca tcagcgatgt cgttctggga
    13801 caactgccag gagtagagcg cctggacggc acactcacga gcgcggcgac gagcagcagg
    13861 tttcacggat ttccccttac taatttcagg ccttgatggc tttcaataca ttaatcattt
    13921 caagcacggt cagtgcagct tctgcacctt tgttgccagc tttggtgcca gcacgttcga
    13981 tcgcttgttc aatgctttca gtggtcagaa ccccaaaagc aaccggaatt tcgctgtcct
    14041 gggcaacatg cgccaggccg ttgcttgcac caccagcgac atattcaaag tgggcagtgc
    14101 caccacgaat aaccgtaccc agcgcaatca ccgcgtcgta tttaccggtt ttagccagtg
    14161 cacccgccgc cagcggcagc tcataggcac caggcaccca aacaacggta atgttttcat
    14221 cttttacctg accgatacgt ttcagtgcgt caattgcacc ttccagcagg ctgtcattga
    14281 taaagttgtt gaaacgcgcg atggtgatgg cgacgcgagc gtccggggta gcaacgttag
    14341 cttcaataat gttcattttc ttccttcggg ttcgagtatg gccccgcagg ggggcggatt
    14401 ttagcataat atttcgtgcg ctgcttccct ttcgagccgg gagatcatgc acccactaaa
    14461 tgcaggcaaa catccgggcc tacatgacgt atctctttga atttaaattg gggggcgtcg
    14521 gctaatttct caagccctgg cagcgagcat aatccacggg cgtcgctgcc taatagttta
    14581 ggtgcgatat agacaatcag ctcatcgact aaacccgcct gtagcaatac gccagcgagc
    14641 gttggccccg cttccaccca gatgctgtta atctgctgtt tacccagttg catcatcagt
    14701 acaaccagat ccagatgacc tttatgctct ggaatcagca aggtacgcac cgtttccggc
    14761 cactcacgag aatcttcctg cgtacgcgcg aaccaggttt cgccgggctg ctgcacaatg
    14821 cgatgttccg gcgtcacgcg attttggcta tcaatcacaa tacgtactgg ctggcggaga
    14881 ttttgttgcg ggtagagcgc ctgagtttgt tcatccagtt cagaccaacg cactgttaag
    14941 gctggatcat ccgccagcac cgtggcgctg ctggttaaaa tggcatgact ttgtgcgcgc
    15001 tgtcgttgta cgtcgcgccg cgcctgaggc gaagtgatcc actggctttc gccgctcgcc
    15061 atcgccgtgc ggccatcaag cgatgcgcca agttttaact gaatataagg aaagccggtg
    15121 cgcatccgct tgagaaagcc tttattcaat tgctcggctt cactcatcat cagcccgtgg
    15181 ctgacgtcaa tgccagcctg ttgcagacgg taaagtccac gcccagcgac ctgcgggtta
    15241 ggatcttgca tcgctgcgac cacgcgcgct acgccagcgg cgatgagtgc gtcacagcac
    15301 ggtggcgtac ggccatgatg gctacagggt tcgagtgtca catacgccgt tgcgcctttg
    15361 gctttttcac ccgccatacg caacgcgtgt acttcggcat gtggttcacc cgctcgttgg
    15421 tggtaacctt caccgacaat ttcgccatct ttgacaatga cgcacccgac attcgggttg
    15481 gggtgggtgg taaaacgtcc tcgttgcgcc agctttagcg cccgcgccat gtaatactcg
    15541 tcctgcacgg cttagtcctc caggcgcgcg atctcttcgc caaattcttt gatatcttcg
    15601 aaactgcggt agacagaggc aaaacggata taggcgactt tatcgagctt tttcaattgc
    15661 tccatcacca gattgccaat catcttgctc ggtacttcgc gctcaccggt ggcgcgcagc
    15721 tgcgatttaa tatgattgat tgccatttca acgtaatcgg aactcaccgg acgtttttcc
    15781 agcgcccgca gcattccgct acgcaatttc tcttcattaa acggttcacg cacgtcgttg
    15841 ctttttacaa cacgcggcat aaccagctcc gccacttcaa aggtggtgaa acgttcatta
    15901 cacaccagac actgccgacg gcggcgtacg gatgaaccct cgcccacgag acgagagtca
    15961 attaccttag tgtccacggc gaaacagaat gggcaatgca tacggcgtcc tgaccaggtg
    16021 gttaacggaa atctatttta ccctgaactg acgatacaac aaaggcgcaa cgcttttgag
    16081 ataaaggatc gatgctttcg ctgcttttac cctctaccat taggctaatt gcgacgagaa
    16141 agggacgacc tatgaatact aacgtttttc gactgctcct gctgggaagc ctgttcagcc
    16201 ttagcgcctg tgtgcagcaa agtgaagtgc gacagatgaa acacagcgtc agcacgctga
    16261 accaggagat gacgcaactc aatcaagaaa cagtcaaaat cactcagcaa aacaggctga
    16321 atgcaaaatc cagcagtggg gtttaccttc tgcctggagc gaaaacaccg gcaagactgg
    16381 aaagccagat cggtacttta cgtatgtcgc tggtgaatat tacgcctgat acagatggca
    16441 ccacccttac actacgtatt cagggtgagt cgaatgatcc cttgcccgca ttcagcggaa
    16501 ctgttgaata tgggcaaatt cagggaacaa tagacaactt tcaggaaatc aatgtgcaga
    16561 atcaattgat taatgcccct gccagcgtcc tcgcccccag cgatgttgat attccgttac
    16621 aattaaaggg gatttctgtg gatcagttag gctttgtacg tatccacgac attcaacctg
    16681 ttatgcagta aacgtatttc gggacgattt tgtgcgtccc gcaacatctt ccccgtcatt
    16741 ttgttactct gcttacatca cctggattga tagtaaaagt ttgcaacaag ggcgaaagtc
    16801 agtacaatcc ccgcccgaat gtgtgtaaac gtgaacgcaa tcgattacgt aaatgataga
    16861 actgtgaaac gaaacatatt tttgtgagca atgattttta taataggctc ctctgtatac
    16921 gaaatattta gaaacgcaat ttgcgccttt ttcactcccg caagggattt tcaaacagtg
    16981 gcatacatat gaaaaaaaca ttactggcag ccggtgcggt actggcgctc tcttcgtctt
    17041 ttactgtcaa cgcagctgaa aacgacaaac cgcagtatct ttccgactgg tggcaccaga
    17101 gcgttaacgt tgtcggaagc tatcacaccc gtttcggacc gcagatccgc aacgatacct
    17161 accttgagta cgaagcattc gctaaaaaag actggttcga cttctatggt tatgcggatg
    17221 cgccggtatt cttcggcggt aactccgatg ctaaaggtat ctggaaccac ggttctccgc
    17281 tgtttatgga aatcgaacca cgtttctcca tcgacaagct gaccaatact gaccttagct
    17341 tcggtccgtt caaagagtgg tacttcgcga acaactacat ttacgacatg ggtcgtaata
    17401 aagatggtcg ccagagcacc tggtacatgg gtctgggtac cgatatcgac actggcctgc
    17461 cgatgagcct gtccatgaac gtctatgcga aataccagtg gcagaactat ggcgcagcga
    17521 acgaaaacga gtgggacggt taccgtttca aaattaaata ctttgtgccg attaccgatc
    17581 tgtggggcgg tcagctgagc tacatcggct tcaccaactt cgactggggt tccgatttag
    17641 gggatgacag cggtaacgca atcaacggta ttaagacccg tactaataac tctatcgctt
    17701 ccagccatat tctggctctg aactacgatc actggcacta ctctgtcgta gctcgttact
    17761 ggcacgacgg tggtcagtgg aacgacgatg cagaactgaa cttcggcaac ggcaacttca
    17821 acgttcgctc taccggctgg ggtggttacc tggtagtagg ttacaacttc tgattatgaa
    17881 aatgccggga tttattcccg gcatttctga ttgttaacaa cgtgtgcttt gttcatgccg
    17941 gatgcggcgt aaacgctttg tccggcccac aaaagcacgt aaattcaata tattgctgaa
    18001 ataacgtagg cctgataagc gtagcgcatc aggcaatttt acgttttaat cacttcttct
    18061 tgttcatcat cgctttcagg tcagcgaatg ggttgtactt cgcttcaccg acatctttct
    18121 gcgcatcttc ccctgcgata acggtcgtac catactgatc cgcttcggta tatttcgaat
    18181 gctcatgatc gtggcaatag agacacaaca attcccagtt actgccatct tccgggttat
    18241 tggtatggtc gtgatcaatg tggtgaacgg taagttcacg caggttagaa taaacaaact
    18301 cgcgggaaca gcgaccgcag acccacggat agatttttaa tgctttttcg cgatagccgc
    18361 tttctaaccg tgcatagttt tttgggatga tagccatgaa aagttacctg gatgaaatgt
    18421 agggttacgc gcagtttccc aagatgcggg gattttcgca atctccatac atcaaaaaaa
    18481 atcccgatct tctgaccggg attttcaaca tcaacgggaa cttgattaag gcagaattga
    18541 cggctgatcc gccccttctt tttccacttt ctgctgcaac atgtgttcgc gcttcatacc
    18601 cagtttcaga gccaacgcag atgctacgta gatggaagat gcagtgccga tggaaacacc
    18661 gataagcatg gtcagcgaga agccttccag taccggacca ccgaagagat acagcatcag
    18721 gataaccatc aaggtagtac cggatgtgat caaggtacgg tgcagcgtct gggtcaagga
    18781 cacgttaaag atttcgtaag gcgtaccgcg acggatcttg cggaagtttt cacgaattcg
    18841 gtccgatacc acgatactgt cgttaagcga gtaaccgata accgacatca acgatgccac
    18901 aatggtcagg tcaatctcga tatggaataa cgacaaaata cccagcgtaa tgataacgtc
    18961 gtgcgtcagc gcaataacca cccctgccgc cagtcgccac tcaaagcgga aacctacgta
    19021 cacgaggata gacagcagcg ctgccatcaa cgccatcgca ccggtttgcg caaggtcagc
    19081 ccccacgctc ggaccgacga actcaatacg cttcactgct gcattctgat tggtggattc
    19141 gttaatcacc ttcagaactt ggctgcccag cacctgaccg ccggtttcgc cttcagcagg
    19201 cggcatacgg accatgatgt cgtggctgct accaaagttt tgcagcattg gctcttcaaa
    19261 accggctttt tgcaatgcat cacgcattac gtcaatttca gccggttttt cgagcgtaat
    19321 ttcgataacc gtaccaccgg tgaaatccag cccccagtta aagccgcgca cgcccataat
    19381 aacgatagca gcgattaaca gcagaccaga gatgccgaaa gcccagtagt cccagcgcat
    19441 aaagtcatag actttacggc cgtggtttag ttgttcaaca gtatattcct gtgccacatc
    19501 gcactcctca gattgacagc ttcttgacgc gcttgccgcc atatagcagg tttacgatgg
    19561 cacgcgtacc gacaatcgcg gtaaacatcg acgtcgccac accgataccg gtagtaatcg
    19621 cgaacccttt aattgccccg gtacccactg cgtacaggat gatgacttta atcagcgtgg
    19681 tgatgttcgc atcgaagata gaactgaatg cgccacgata accttcatca attgcctgtt
    19741 gaacagtacg tccgttgctc aactcttctt taatacgttc gttgatcagt acgttcgcat
    19801 cgaccgctac cgcaagggtt aagacgatac ccgcgatacc tggcatactc agtgttgcgc
    19861 ctggcaacag cgacataata ccgacgatca agatcaagtt ggcaatcaga gcactggtcg
    19921 caatcagacc aaacttctta tagaagatga tcatgaacag aatagaaacc agcagaccgg
    19981 caaggcaagc ctccagcccc tgctcaatgt tctgcatacc cagggtcggg ccaattgtac
    20041 gttcttcaac aatctgaatc ggcgcgatca acgcaccggc acgcagcagc agtgacagct
    20101 gacgggcttc gttcgggttg ttgatgccgg tgatacggaa gctgttaccc agacgagact
    20161 ggatgttggc gatgttaatc acctcttcct gtttcaccag aaccgcacga ccatttgcat
    20221 ctttcttacc gctgtctttg tactccacaa acagggttgc catcggtttg ccgatgttgt
    20281 ccttggtgaa gttagacata atgttgccac cagcgctatc gagcgagatg ttaacctgcg
    20341 gctggttgta ttcgtcctgg ctggaagtgg agtcggtgat atggtcacca gtcagaatta
    20401 cgcgtttgta cagcacaacc ggctgacctt cgcgggtctg tttcacttca gagtcgcccg
    20461 gtacgcgacc ggatgccgca gcggcctgat caacattggt gtttaccaga cggaattcca
    20521 gcgttgccgt cgcgcccaga atctctttcg cacgcgcagt gtcctgaata ccgggcagtt
    20581 caacaacgat acggtcagca ccctgacgct gaaccaccgg ctccgccacg ccaagttggt
    20641 ttacacggtt acgcaggata ttaatgttct gctgcaccgc atattcacgc gcttcactca
    20701 gacgagcatc gctcattacc gcacgcagct ggttgctgcc ctggctgcta atcaccaggt
    20761 ccggatggcg cttgctcaga tacgcaatgg cttcatcacg agctttagca tcgcggaaag
    20821 tgatgctcag gccgtagttg ttttctttac gaacagtggt atacgggatg cccttttcgc
    20881 gcaggtcact gcgtaggcta tcgatatttt gttcctggag tttgccaagc gcggtgtcca
    20941 tatccacttc catcaggaag tgaacgccgc cacgcaggtc aaggccgagc ttcatcggct
    21001 cagcatgaat agctgccagc cagcgcggcg ttgccggggc aaggttaagc gccacgacgt
    21061 atttgtcacc cataacgccc attaatgctt cacgagcgcg caactgggtg tcagtggagt
    21121 cgaagcgcgc aagaatagcg ccctcttcca gtgccacaga cttagcagtt attttttctt
    21181 cttgtaacgt tttctggacc tggatcagcg tttgctcact ggcggcgact ccgcgcgcac
    21241 cagtgatctg aacagccgga tcctcaccaa acaggttggg aagcgcatac agcagaccga
    21301 tgacaatcac cacgatcagc atgacgtact tccacaaagg ataacggttt aacacggcaa
    21361 ttcccttagg gaaaaatttt aattacagcg ccttcatggt gcctttcggc aggacggcag
    21421 ctacgaagtc acgtttaata actacttcag tggtgtcatt cagcgcgata gcaatgtagc
    21481 cgttttccgc tactttggtt acgcgaccaa ccaggccacc gttcgtcaga acttcatcac
    21541 ctttggcaat ggagtccatc agctttttgt gttctttggt gcgcttctgc tgtggacgca
    21601 ggatcatgaa atagaaaatc agaccgaata ccaccagcat caaaatcaaa gacatcgggc
    21661 taccttgcgc cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag ctcattaaat
    21721 ttccctcatt attaatatta atcaacgttc aaaggtggta cttctcgccc ctgacgctgg
    21781 taaaaatcag ttacgaagct ctctaattta ccctcttcaa tagccttgcg taaacccgcc
    21841 atcaaacgct ggtagtaacg aaggttatga atggtgttga gtcgcgcgcc taatatttcg
    21901 ttgcaacggt cgagatgatg caagtaagcg cgtgaataat tgcgacaggt gtagcaatca
    21961 cactcaggat cgagtgggcc agtatcgctc ttatatttcg cattgcggat tttcaccacg
    22021 ccatcggtca cgaacaaatg accatttcgg gcgttgcggg ttggcattac gcagtcaaac
    22081 atatcgatac cgcgacgtac gccttcaacc aggtcttccg gtttaccaac gcccatcagg
    22141 taacgcggtt tgtctgccgg aatttgcgga cacacatgct ccagaatacg gtgcatatct
    22201 gctttcggct cacccacagc cagaccgccg acagcgtagc catcaaagcc gatatctacc
    22261 agacctttaa cagatatatc acgtaaatct tcgtaaatac tgccctgaat tataccaaat
    22321 aacgcatttt tgttaccaag actgtcaaaa cgctcacggc tacgcttcgc ccaacgcaga
    22381 gacatctcca tagagcgttt tgcgtaatcc cagtcagcag gatacggcgt acactcatca
    22441 aagatcatga cgatatccga accaagatcg tactgaatct ccatcgactt ttccgggtcg
    22501 aggaaaatcg ggtcgccgtt gatcgggtta cggaagtgaa cgccctgttc ggtgatttta
    22561 cgaatatcac caaggctgaa gacctggaag ccgccggagt cggtaagaat cggtcctttc
    22621 cactgcataa aatcgtgcag atcgccgtgc agtttcatga tttcctggcc cgggcgcagc
    22681 cacaggtgga aggtgttgcc gaggataatt tgcgcgccag tggcttcaac ttcttccggc
    22741 gtcatccctt ttacggtgcc gtaggtgcca acaggcataa aacaaggcgt ttccactacg
    22801 ccacgatcaa agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc cagttcaaat
    22861 ttcatttttt ctccactacg tcagaaaaac agtccaacgt ttaaaccagc gccgcggaat
    22921 tactccccga cgcgctcatt aattgcctgc ggattgtacg tgataaacat cgcatcaccg
    22981 taactaaaaa agcgatattt ctcttctacc gccgctttat aggcattcat ggtgtgttga
    23041 taaccggcaa aggccgaaac cagcataatc agcgtcgact ctggcaagtg gaagttcgtc
    23101 accagcgcat cgaccacttt gtactggaag cccggataga taaagatttg ggtatcgtca
    23161 aagaacggtt cgataagatc gttttttgcc gcctgtgccg cgctttccag cgaacgtact
    23221 gaagtggtgc caaccgcaat cacccggtta ccgcgcgctt tcgccgccag taccgcgtct
    23281 accacatcct gcggtacttc agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg
    23341 acgcgcaccg gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc catctccacg
    23401 cctttggcgc gtaatttttc cagcaaaggc tcgtcaaaat gcagaccagc ggtcggggct
    23461 gcaaccgcgc ccggtttttc gctataaacg gtttgataaa gttcgcggtc agcgtcttcg
    23521 tccggacgat cgatatacgg cggcagcggc atatggccga tgctgttgag gatatccagt
    23581 accgagcgtt catcattaaa ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt
    23641 gcgttgatac tttcgtcatc gcccagcaac agttctgcgc caggttttgg cgctttcgag
    23701 gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc gctcaaccag cacttcaatc
    23761 ttgccgccgc tggctttacg cccaaacaga cgcgccggga tcacgcgggt attattaaaa
    23821 accagaagat cgccggggtt gagcttatcg agtaaatcgg tgaaagtacc gtgcgtcagc
    23881 gcgcccgtcg gcccgtccag cgacagtaaa cgacagctac tgcgttcagg catgggatag
    23941 tgggcaatca gggattcggg caattcaaag gagaaatcgg taacgcgcat gacactgact
    24001 cagactaaaa taagagtcgg gtagtctagt gccggggcgc tctccctgca acctttaccc
    24061 ccctttggat agaatataat cgatgaattt tttagctcac ctgcatttag cccatctcgc
    24121 ggaaagctcg ctttctggca atttactggc tgatttcgta cgcggaaatc ctgaagaaag
    24181 ttttccgccc gacgtcgtgg ctggcattca tatgcatcga cgtattgact gacaatctgc
    24241 cggaagtccg cgaagcacag gagtggtttc gtagtgaaac gcgccgcgtt gcgcctatta
    24301 cgctggatgt catgtgggat cactttcttt cccgccactg gtcgcagctg tcgccggact
    24361 ttccgctaca ggaatttgtc tgttatgccc gcgagcaagt gatgacgatt ttgccggact
    24421 caccgccacg ttttatcaat ctgaacaatt acttgtggtc agagcagtgg ctggtgcgct
    24481 atcgcgatat ggatttcatc cagaacgtgt taaacggcat ggcaagccgc cgcccacgtc
    24541 tggatgccct gcgtgactcc tggtacgatt tagacgctca ttatgacgcc ctcgaaaccc
    24601 gcttctggca gttttatccg cggatgatgg cgcaggcgtc acacaaggcg ttataaaacg
    24661 ttagttcatc cataccgtag ccgaaatggc aggcaaagcc agaatcccgt cagtcagttg
    24721 cccatgccct tctttgcatt gccattgcac ggcattgaga aacggtgacg cgggtagcac
    24781 cacttcactg gcctcgccac ggttgattgc caccagtaca cgttgctgat tcagcacgcg
    24841 gacaaatacc actacgttat cttccgcata cagcacctga cagccgccac gacgcagcgc
    24901 ctgacttttc ttacgcagca caatcattcg ccggtacagc gcgaataacg ccgtatcctg
    24961 cttttccacc tgccagggga acggtttacg gcaaaacgga tcgtttttac catccagtcc
    25021 cacttcatca ccgtaataaa tgcacggtac accaggccag gtgaacagcc agaccaccgc
    25081 cagcggcagg cgcgcaatat cccgaccgag cagcgtttta aatcgcgcag tatcgtggct
    25141 atcgagctga ttaaacatac gtaattgttg ttgatgagaa agccctgcgc ggtaattatc
    25201 catccaggcc atacaggttt gggcgtcaat ttgctgcgga tcataagaga tgtcggtatt
    25261 ggcaagaaat ccccacaacg ggaatgtgaa gccacgatag ttcatggcag catcttccac
    25321 atcggcctgt aaccattgcc gtgcatcgcc aaaatgttcg ccgacaatat acgcttccgg
    25381 ctgggtttct ttcgccgctt cggtgatccc ggcaacgtgc tgcatattat tgcgcgcccc
    25441 acccgcctcc cccagcatat gcaccacatc cagccgccag ccgtccatat tccacggcgc
    25501 tttcagccag tggcggacaa tactgtcttc cccgcgataa atttcattca ccagactttc
    25561 cgactgataa tccagcttcg tcaagctggc atagccaagc cagtcgagcg ccgtgccatc
    25621 atcactaaac gagtaccagt cgcgccaggg cgattcagag ttgtgacaag ctccccccgt
    25681 gccaagattg tgcctgtcaa accaggcatg ggaatcgcca ctgtggttaa acacgccgtc
    25741 cagcaccagc cgcattccca gctgctgcgt attgtgtcgc aaacgcagca acgccccatc
    25801 accgccaaac tgcggatcga catggcgata atcctcggta tcgtatttat gtacgctggg
    25861 agctttaaac accggattga gatacagcgc tgtcacgcca agctttttca gatacggcag
    25921 tttttcgctt atcccgtcca gatcgccgcc atagaacgtt gatccgcccg cctgcgccgt
    25981 gaccggttca tcccagtcac gcaagatgat ctcttgtccg gctgcatgat ggtaatagac
    26041 atgatcctgt tcagcttcac gaggaagact acgcgcaaaa cgatcaggga agatctgata
    26101 aaaaatctga tccgcagccc attgtgggcc gatatcaggt acatcgacgg caaactgctc
    26161 cagtcgtgcc ggcggcattc ggctgaagcc ctgcggtgta aaccaacgct ggcgatcgtg
    26221 ccacagcagt ttgaaactgt aacgccgccg gggttgtccg ctggagagat caatcgccgc
    26281 acgccatgcg gtgacgcctg gctgcggctg actgcgctgc ttatgcattg gtactgacat
    26341 ttcttcgtta tcgtgttctg tacgcagcat aatgcgctgc ggtgggtctt cgcccgtcag
    26401 ccacagtgta atgagcagtt gatctttgct ttgtttaaca aatgggggca ccggcaggtg
    26461 ccatgcattt aacatcataa ttcccctgtg atgaaaatct gatcaccttg ccatagccta
    26521 atgcaggatc actcatcatc tgaaggatat ttgggggagg aggacggggg cggataactt
    26581 tcggaatagc cggatcagga tttcctcctg aaccggctta cagaacgcaa gtattattgg
    26641 ttttcagcca gctgacgatc gtggcggcgt ttaaacatcc agccaatcaa cagcacaaca
    26701 atccacgcga aaccgacata cagcgagata cgcgtatccg ggtgataacc aatcaacccg
    26761 ataataaaga gcaggaaaat cagcccgccg atggtcgttg ctaccccacc cggcacttta
    26821 aatttcagcg ccttaacttc ttctggcggc aaacggcgac ggaaggcgat ttgcgacagc
    26881 aggatcataa tccacaccca taccgtggcg aaggttgcca gcgaagcgat caccaggaag
    26941 acgttttccg gcatgatgta gttcagatac accgcaaaca gcagcgcggt agtcatcacc
    27001 agcaccgtaa cccacggaat accgcgacgc gacgttttgc tgaaaatttt cggtgcgctg
    27061 ccctgctctg ccataccgtg gagcatacgg cctacgccaa atacgtcact gttaattgcc
    27121 gacagcgaag cggtcagcac aacaaagtta agaatgctgg cggcaaaggt aatgcccata
    27181 tgctggaacg tcagtacgaa cgggctaccg gcagtgccaa cctgattcca cgggtagata
    27241 gacataatga cgaacagcgt accgacgtag aacaccagaa tacgcatcgg cacggagtta
    27301 atcgcacgcg gaatcgattt ctcaggatct ttcgcttcac cggcggtaat cccgataatt
    27361 tcgatcccac cgtaagcaaa catcaccatt tgcaacgaca ttaccatgcc aagccagccg
    27421 ttactgaaga agccgccgtt gctccacagg ttatgaatac cggtcggttg cccgccgttg
    27481 ccaatccccc agatgatgat gccgaaaccg gcgacaatca tgatgatgat ggtggcgact
    27541 ttaaagaacg agaaccagaa ttccagctca ccgaagacct tcacgctcat caggtttacg
    27601 gcgcaaatga tcagcaccac gctcagtacc caaatccagt gcggcaccgt cgggaacaag
    27661 acacccatat agataccaaa agcggtcaca tcggcgatgg cgacaataag gatttcaaaa
    27721 cagtaggtcc atccggtaat gtaacctgcc agcgggccaa ggttttcctg cgcataacgc
    27781 gagaaagagc tggcggccgg gttatgtacc gacatttccc ccagcgcacg cataatgata
    27841 tacgccgcga taccaccgat aatataggcc aacaacacgc tcggaccggc cattttgatg
    27901 gcgtctgccg aaccgtaaaa cagcccggtg ccaattgctg aacccagtgc cataaagcgt
    27961 atgtggcggg tacttagccc acgctttagc ttattcttac tttccatcaa ttcctaaccc
    28021 atcaacacaa taaaaaacca cggggcatcg cagccccgtg gttaaaacaa atgttcagtg
    28081 atttagtgag cgctggaggt cacctgacga cctgccgcgc gatcccagat aatggccaga
    28141 accaccatca ccactgttgg cattaaccac gccagacctt gttcggccag cggtaaacgc
    28201 tgcgcccagg acggtaagat atcgctgaat gcagatgctt tgatcccgtc gagaatacca
    28261 aaaagcaggc tgataaacat cggcggagca atcacgcggg acgaattatg ccaccatgag
    28321 cgtgtaaaac ttaatacaac cagtgcgata cacggcggat aaatagcggt cagcaccggt
    28381 acggagatct ggatcagctg gcttaagccg aggttagaca ccaccatcga gaagccgccg
    28441 aggataaaca ccagcgtacg ataagagagc ggtacgtact gggcaaagaa ttctgcacaa
    28501 gcacaggtca ggccaaccgc cgtgaccaga caggcgatga agattaacgc cgccagcagg
    28561 aagctaccgc cgccgccaaa ggtgtgctga acgtaagcat gcagaatagc agcgccgttt
    28621 gcagactgat cgaccagcga cgcgctgtct gaacccagac agaacagcgc caggtacagc
    28681 aaagtcagac caacacccgc catcaggcca gcccagacgg tataacgggt cagcagacgc
    28741 gcttcggtaa cgccacgaga acgcgccgcg ttaacaataa cgataccaaa caccattgcg
    28801 cccagcgtat ccatggtcag atagccgtta acgaagccgt tagaaaatgc agcgttttga
    28861 taagcctcag tcgccgtgct gatagaaccc gccggccaga caatcgcgac aacagacagg
    28921 atgaccagcg cgataatttt cagcggcgca aggaagttgc ccacggtatc cagcagcttg
    28981 cccggataga gcgaaaccag aataacgata gcgaaataga ccaggctgta gataaacagc
    29041 ggcagcgcgg aatcacccgt cagcggcgca atccccactt caaaggaaac ggtagctgta
    29101 cgcggcgtag cgaaaagcgg ccccaccgcc aggtaacaaa ccgttgccag cagtacgcca
    29161 gcgactttac cgattggcgt gctgaggctg tcaacaccgc cgccaacttt tgccagcgcc
    29221 actaccgtta ataccggcag gccaacggca gtaatgagga agccgaatgc cgcagtccag
    29281 acgtgttcgc ctgcctgtaa gccgaccatt ggagggaaaa taatgttacc tgcgccgacg
    29341 aacaacgcaa atgtcataaa gcccagagcg atgatatcgc gcgatcttaa ttgatgggtc
    29401 ataaatcata ctgcctgtgg atgtggtgtt gtaacgttaa atttttgtgc caatccctgg
    29461 ggatgataca gcggtatatt taaacaattt tagcgggaaa tactcccaac caggcgcggc
    29521 gaagaatagt ttttcgattt accgcgttaa tgcagtcagt atgacagcac ctgaagcgca
    29581 atttaaacgc ttataacgtt ttaaagcaag gagggatcta aaaaccagag gtatatagcg
    29641 ataagaaatc accaaccagt ataaaacact atttccgtga aaaagacatg gctaatcatg
    29701 cgaacaaaaa atcaccaacc gttgtaaaat ctaacgcccg tattttgact gcaaagaaaa
    29761 taagccagca tcggctggct tatggcaata aaagatgaca aaggcggatt aatcgctgtt
    29821 tttggcaatt aaacgttccg ggataacaaa actgaaacgt gttccttttc ctactgtact
    29881 ctcaatattc aggcgacttt cgtgatgatt caccgcatgt ttcacgatcg ctaaccctaa
    29941 tccgctaccg ccggtttgcc gggaacgcgc tttatcaacg cgataaaaac gctcggtcag
    30001 gcgcggaata tgctccggtg caatgcccgg tccgttatct tcaacgctaa attcggcacc
    30061 gtgcggcact cgctgccagc gtacggtgat atgcgtgcct tccggcgtat gattcacggc
    30121 gttatagacc aggttcgaaa tcgcactgcg tagctgatct tcgttgccag acaccttgag
    30181 gccgttatct atctcaaagg taaatgtctg ttttttctga ctcagagtct gagcctcgcg
    30241 ctcaacaacg cgcagcatca tcggcacatc aaccttttca ttgagcaatt gcgtcggcgc
    30301 ggcttcaatt ttcgacagcg ttagcaactg cttcaccagc ccttccatcc gctgggtctg
    30361 ctcgcgcatg gtgtgcaacg ctttttcgcg taccgcgcct tccagcggct gctcatccat
    30421 catctccagg taaccctgta acacggtcaa tggcgtacgt aactcatggc tcacgttggc
    30481 aaaaaagtta cgccgcgccc cttccagttg atgcatttgc gtgacatcac gcgccaccat
    30541 cagcaactgt ttgtgggtat aaggcatgac gcgaatttcc agatgccgcc cggtgttgag
    30601 caccagattg agcgggcgag aaaaatcacg cgttttcaga tattgcgtaa actccgggta
    30661 acgcagtagg ttaaggatgt tctgcccgtt atcttccggc cagcgcaaac caagaatttg
    30721 ttgcgccaga ccgttacacc agaaaatacc gccctcttcc gtggtcagca ccaccgcgtc
    30781 gggcagcgac tccgcgccgc tacgaaagcg tttaatcaga ttgcccagtt cacggcggcg
    30841 ttttttattt cgcagctgca tctggtgtaa gccgtatagt agcggttccc agctaccacg
    30901 ccccggtggc ggggtcatac tgcgatccac ccacagccac catgaaaggc gcaataaatt
    30961 ccagaaatgc cagataagca gtcctgttac cgatgccagc aaaaaccagg gcaggtaacc
    31021 aaaaaatgca cccaggatga aagccgggag gcagcaaagt agcagctcca gcaccagcct
    31081 tttccacgac agccgttcca gcacgtaaga tactccagtt aagaaatcat aagccctgct
    31141 ctgcgtccga tgagcaaggc gttaaaagcg ggttgaaaaa cgatatcctg taccgcgcac
    31201 ggtctgcacc atgcggtcat gcccgccggg ctccagtgct ttacgcaggc gacgaatgtg
    31261 gacatcgacc gtgcggtctt ccacatacac gttagttccc cagacgtggt ttaacagctg
    31321 ctcgcggctg tacacgcgct caggatgcgt cataaaaaag tgcagcagtt taaattctgt
    31381 cggccccatc tccagcggct cttcgcccgc catcactcgg tgagatgtcg ggttgagact
    31441 taatccctgc atcttaatca cctcttccac cgccattggc gaaatacggc gcattaccgc
    31501 tttgattcgc gccaccagct ccttcggcga aaatggcttg gtgatatagt catccgcgcc
    31561 ggtttcaagg ccgcgcacgc gatcttcttc ttcccctctg gcggtcaaca tcaccactgg
    31621 aatatcccgg gtcatcgact cgcgcttgag gtgtttgatg aactggatac cggagccgcc
    31681 aggtaacatc cagtcaagga gaattaaatc cggccagggt tcattcagtt gattcacagc
    31741 actgtcataa tcttccgctt cgaccggctg aaagccattt tgttcgagca cgaagcagac
    31801 catttcgcga attggagctt catcttctac gaccagaata cgtctcgcca tgatttgccc
    31861 tgttgtaata aataggttgc gatcattaat gcgacgtcat tatgcgtcag atttatgaca
    31921 gatttatgaa aagctcgtcg cacatatctt caggttattg atttccgtgg cgcagaaaaa
    31981 agcaaatggc acatctgttt gggtataatc gcgaccatgc tttttcgcca gggaaccgtt
    32041 atgcgcatcc ttcacacctc agactggcat ctcggccaga acttctacag taaaagccgc
    32101 gaagctgaac atcaggcttt tcttgactgg ctgctggaga cagcacaaac ccatcaggtg
    32161 gatgcgatta ttgttgctgg tgatgttttc gataccggct cgccgcccag ttacgcccgc
    32221 acgttataca accgttttgt tgtcaattta cagcaaactg gctgtcatct ggtggtactg
    32281 gcaggaaacc atgactcggt cgccacgctg aatgaatcgc gcgatatcat ggcgttcctc
    32341 aatacaaccg tggtcgccag cgccggacat gcgccgcaaa tcttgcctcg tcgcgacggg
    32401 acgccaggcg cagtgctgtg ccccattccg tttttacgcc cgcgtgacat tatttccagc
    32461 caggcggggc ttaacggtat tgaaaaacag cagcatttac tggcagcgat taccgattat
    32521 taccaacaac actatgccga tgcctgcaaa ctgcgcggcg atcagcctct gcccatcatc
    32581 gccacgggac atttaacgac cgtgggttcc agtaaaagtg acgccgtgcg tgacatttat
    32641 attggcacgc tggacgcgtt tccggcacaa aactttccac cagccgacta catcgcgctc
    32701 gggcatattc accgcgcaca gattattggc ggcatggaac atgttcgcta ttgcggttcc
    32761 cccattccac tgagttttga tgaatgcggt aagagtaaat atgtccatct ggtgacattt
    32821 tcaaacggca aattagagag cgtggaaaac ctgaacgtac cggtaactca acccatggcg
    32881 gtgctgaaag gcgatctggc gtcgattacc gcacagctgg aacagtggcg cgatgtatcg
    32941 caggagccac ctgtctggct ggatatcgaa atcactactg atgagtatct gcatgatatt
    33001 cagcgcaaaa tccaggcatt aaccgaatca ttgcctgtcg aagtattgct ggtacgtcgg
    33061 agtcgtgaac agcgcgagcg tgtgttagcc agccaacagc gtgaaaccct cagcgaactc
    33121 agcgtcgaag aggtgttcaa tcgccgtctg gcactggaag acctggatga atcgcagcag
    33181 caacgtctgc agcatctttt caccacgacg ttgcgtaccc tcgccggaga acacgaagca
    33241 tgaaaattct cagcctgcgc ctgaaaaacc tgaactcatt aaaaggcgaa tggaagattg
    33301 atttcacccg cgagccgttc gccagcaacg ggctgtttgc cattaccggc ccaaccggtg
    33361 cggggaaaac caccctgctg gacgccattt gtctggcgct gtatcacgaa actccgcgtc
    33421 tctctaacgt ttcacaatcg caaaatgatc tcatgacccg cgataccgct gaatgtctgg
    33481 cggaggtgga gtttgaagtg aaaggtgaag cgtatcgcgc gttctggagc cagaatcggg
    33541 cgcgtaacca gccggacggt aatttgcagg tgcctcgcgt agagctggcg cgctgcgccg
    33601 acggcaaaat tctcgccgac aaagtgaaag ataagctgga actgacagcg acgttaaccg
    33661 ggctggatta cgggcgcttc acccgttcga tgctgctttc gcaggggcaa tttgctgcct
    33721 tcctgaatgc caaacccaaa gaacgcgcgg aattgctcga ggagttaacc ggcactgaaa
    33781 tctacgggca aatctcggcg atggtttttg agcagcacaa atcggcccgc actgagctgg
    33841 agaagctgca agcgcaggcc agcggcgtca cgttgctcac gccggaacaa gtgcaatcgc
    33901 tgacagcgag tttgcaggta cttactgacg aagaaaaaca gttaattacc gcgcagcagc
    33961 aagaacaaca atcgctaaac tggttaacgc gtcaggacga attgcagcaa gaagccagcc
    34021 gccgtcaaca ggccttgcaa caggcgttag ccgaagaaga aaaagcgcaa cctcaactgg
    34081 cggcgcttag tctggcacaa ccggcacgaa atcttcgtcc acactgggaa cgcatcgcag
    34141 aacacagcgc ggcgctggcg catattcgcc agcagattga agaagtaaat actcgcttac
    34201 agagcacaat ggcgcttcgc gcgagcattc gccaccacgc ggcgaagcag tcagcagaat
    34261 tacagcagca gcaacaaagc ctgaatacct ggttacagga acacgaccgc ttccgtcagt
    34321 ggaacaacga actggcgggt tggcgtgcgc agttctccca acaaaccagc gatcgcgagc
    34381 atctgcggca atggcagcaa cagttaaccc atgctgagca aaaacttaat gcgcttgcgg
    34441 cgatcacgtt gacgttaacc gccgatgaag ttgctaccgc cctggcgcaa catgccgagc
    34501 aacgcccact gcgtcagcgc ctggtcgcgc tgcatgggca gattgttccc caacaaaaac
    34561 gtctggcgca gttaatggtc actatccaga atgtcactct ggaacagacg caacgtaatg
    34621 tcgcacttaa cgaaatgcgc cagcgttata aagaaaagac gcagcaactt gccgatgtga
    34681 aaaccatttg cgagcaggaa gcgcgcatca aaacgctgga agcccagcgt gcacagttac
    34741 aggcgggtca gccttgtcca ctttgtggtt ccaccagcca cccggcggtc gaggcgtatc
    34801 aggcgctgga gcctggtgtt aatcagtctc gattactggc gctggaaaac gaagttaaaa
    34861 agctcggcga agaaggtgcg gcgctgcgcg ggcaactgga tgcattaacg aagcagcttc
    34921 agcgcgatga aaacgaagcg caaagcctcc gacaagatga gcaagcactt actcaacaat
    34981 ggcaagccgt cacggccagc ctcaatatca ccttgcagcc acaggacgat attcaaccgt
    35041 ggctggatgc acaagatgag cacgaacgcc agctgcggtt actcagccaa cgacatgaat
    35101 tacaagggca gattgccgcg cataatcagc aaattatcca gtatcaacag caaattgaac
    35161 aacgtcagca gcagctctta acggcattgg cgggttatgc actgacattg ccacaggaag
    35221 atgaagaaga gagctggttg gcgacacgtc agcaagaagc gcagagctgg cagcaacgcc
    35281 agaacgaatt aaccgcgctg caaaaccgta ttcagcagct gacaccgatt ctggaaacgt
    35341 tgccgcaaag tgatgatctc ccgcacagcg aagaaactgt ggcgctggat aactggcggc
    35401 aggttcatga acaatgtctc gcattacaca gccagcagca gacgttacag caacaggatg
    35461 ttctggcggc gcaaagtctg caaaaagccc aagcgcagtt tgacaccgcg ctacaggcca
    35521 gcgtctttga cgatcagcag gcgttccttg cggcgctaat ggatgaacaa acactaacgc
    35581 agctggaaca gctcaagcag aatctggaaa accagcgccg tcaggcgcaa acgctggtca
    35641 ctcagacagc agaaacgctg gcacagcatc aacaacaccg acctgacggg ttggctctca
    35701 ctgtgacggt ggagcagatt cagcaagagt tagcgcaaac tcaccaaaag ttgcgtgaaa
    35761 acaccacgag tcaaggcgag attcgccagc agctgaagca ggatgcagat aaccgtcagc
    35821 aacaacaaac cttaatgcag caaattgctc aaatgacgca gcaggttgag gactggggat
    35881 atctgaattc gctaataggt tccaaagagg gcgataaatt ccgcaagttt gcccaggggc
    35941 tgacgctgga taatttagtc catctcgcta atcagcaact tacccggctg cacgtgcgct
    36001 atctgttaca gcgcaaagcc agcgaggcgc tggaagtcga ggttgttgat acctggcagg
    36061 cagatgcggt acgcgatacc cgtacccttt ccggcggcga aagtttcctc gttagtctgg
    36121 cgctggcgct ggcgctttcg gatctggtca gccataaaac acgtattgac tcgctgttcc
    36181 ttgatgaagg ttttggcacg ctggatagcg aaacgctgga taccgccctt gatgcgctgg
    36241 atgccctgaa cgccagtggc aaaaccatcg gtgtgattag ccacgtagaa gcgatgaaag
    36301 agcgtattcc ggtgcagatc aaagtgaaaa agatcaacgg cctgggctac agcaaactgg
    36361 aaagtacgtt tgcagtgaaa taactattca gcaggataat gaatacagag gggtgaatta
    36421 tctcttggcc ttgctggtcg ttatcctgca agctatcact ttattggcta cggtgattgg
    36481 tagccgttct ggtggttgtg atggtggtat gaaaaaagtc attttatctt tggctctggg
    36541 cacgtttggt ttggggatgg ccgaatttgg cattatgagc gtgctcacgg agctggcgca
    36601 taacgtagga atttcgattc ctgctgccgg gcatatgatc tcgtattatg cactggtggt
    36661 ggtggtcggt gcgccaatca tcgcactctt ttccagccgc tactcactca aacatatctt
    36721 gttgtttctg gtggcgttgt gcgtcattgg caacgccatg ttcacgctct cttcgtctta
    36781 cctgatgctc gccattggtc ggctggtatc cggctttccg catggcgcat tttttggcgt
    36841 cggagcgatc gtgttatcaa aaattatcaa acccggaaaa gtcaccgccg ccgtggcggg
    36901 gatggtttcc gggatgacag tcgccaattt gctgggcatt ccgctgggaa cgtatttaag
    36961 tcaggaatgc tggcgttaca cctttttatt gatcgctgtt tttaatattg cggtgatggc
    37021 atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc tgcgcgaaca
    37081 atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga tgtttggcaa
    37141 cgcaggtgtg tttgcctggt tcagctacgt aaagccatac atgatgttta tttccggttt
    37201 ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg tgctgggaaa
    37261 tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag cagtgactga
    37321 ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga aaacaacgtc
    37381 gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac ctctgcaaat
    37441 attgttactg caaaatgcca aaggaggaga gttattaggt gccgcaggtg ggcaaatagc
    37501 gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga cgctggggct
    37561 ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga tgtcatcgtt
    37621 gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc tggcgaaacc
    37681 actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc gtgaacgcct
    37741 tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg caagcgtcgc
    37801 atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa ccacgcagcg
    37861 ccgcgtacgc cgctggaatc accgtgcttc gccttactca ccggcgtttc acattcgccg
    37921 ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc tacattgctc
    37981 atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg tgccagcgat
    38041 tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc gctttcttca
    38101 accaggcgga taatttcact gcctttcagc gcatgtccac tcaaacgacg ataatccgtc
    38161 gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca ataacaaggg
    38221 acttcctcgc gataacgcag ttcgtcttcg tccatccacg gtagcggatt gtgtccccac
    38281 tcacctgccg tgccattgcc gccgatatgc gtccgcccat tgaatgccac gcccgcgccg
    38341 catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc gccatctact
    38401 gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg ctgcaacctc
    38461 gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattgac attcttcacc
    38521 acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc gcgctgccct
    38581 gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg ccggtaatca
    38641 tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc cagtgcaatc
    38701 acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc cttatttttt
    38761 tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg ccgacaattc
    38821 gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct gtggttcaaa
    38881 aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga gatggaaaaa
    38941 cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa gatgggctgg
    39001 gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg tcaaattgtt
    39061 atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca ggcgctggaa
    39121 gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac cgaaaaagat
    39181 tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg ttttagccag
    39241 acaatgatgt ggctcgacac ggttaacggt ttgattatgg tggactgcgc cagtgccaaa
    39301 aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc ggttgtacca
    39361 ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc cggtagtgcg
    39421 gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga agatggcggc
    39481 gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca cattgaagcc
    39541 ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt tgtgatgtgc
    39601 gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca aaacgaagat
    39661 atcgaccgtg aagatttcgc ccagcgtttt gatgccgatt tcatcctgat gactggtgaa
    39721 ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca acgttaattg
    39781 ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg tacgaagcca
    39841 caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc aatattatgc
    39901 gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc agcacgcaga
    39961 atattcacta acccccattt atcgctggta aattgttttg ttaacagctc cgcttttggg
    40021 gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag gtaaggatac
    40081 cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa gtctttcggg
    40141 taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc ggtacttcca
    40201 ccattttggc tgcgcatggg ataacgggta cagtgaattt gcaggttgat cgttggcgtc
    40261 agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat tgtttttcct
    40321 caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc actaaatcac
    40381 gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatggatcc tcttttaacc
    40441 acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac cgatcctgag
    40501 gctcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat gccgtttggc
    40561 gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct gatttatctt
    40621 ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt atatcaacct
    40681 tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaaggaaat attattatgg
    40741 gttaaagaga ggatatttca gaaaaatgcc ctcccgttca aggggaaggc gaggaattac
    40801 agatagcggc acagataaga ggtaggttcg gcaacttgaa gatgaaactc actgtgaccg
    40861 ggaacattaa acaccgaacc ggcttcatac acttgccagt cggtcgcgtc aggcagtaac
    40921 acattcagcg cgccactgat taccgtcatc tcttccggct cagcggtgct gaaggtgtat
    40981 tcgccttcaa ccataacacc cacgctggcg cgaccagtgc tgctgctgga aaagccgatc
    41041 gatttcactt tgccggaaaa gtactcatta ctttgaagca taaactggcc ctgtctggta
    41101 gttgaacatc tacttcacta taggggccag aggcgctggc tgtcacgcaa aattacacca
    41161 gtaattccgc agccagccgt gcaatcaata cgttagagag caagacagga acatcgagct
    41221 gtttttgcag taaatcgcga tgacgttggt gaaatcctaa acaatccagc atgatgacat
    41281 cagccccttt tgccagtaat tctttcccag catcaatgat tttttgttct gaatcatgaa
    41341 tggggttacc caatgaaaat accggcgatt tctgcaaaat ttgccatttt tgcgcctgaa
    41401 cgggcagcat ctcctcaacc ggaacgataa cccccacctg atgatcttca acaatagagg
    41461 aaaccagtgg aggcaatatt cgcgacggct caagaaagat cgtattacgc gcagtcatac
    41521 tactaatgtt tgctgtactc attaataaaa tgacgtcata accctgatta tcgagcactt
    41581 caaccacacc ttgcagatct cgctccactt tacgacgcga aacatgggcc agctggttgt
    41641 catttaataa tgtgagaatg gtgtcttcgc ctgcttctgg cgcgtactcc gccatcactt
    41701 cttcacgact taacttcccc agcaggctat gatgggaaat attatcttcg tcaatgtatt
    41761 ccgtcaggag cggcaaaacc tcctgcatag gtacaatgcc gatggtcagg atcgccaacg
    41821 acgcactcat tgttcatcca ccttactttt ctttcgtcgt taattaccgg gcaagtgtga
    41881 agcaccactg ctgacactta cttgtgctac aaatgacaaa aagcgtagca gcagacgtgc
    41941 acggcataat gtaaagattc caaatgattc cagtaatgga atgttattgt ttaatattct
    42001 aattattaga aaaacataaa ttatgaaaaa tgtgacgcag atcccttgta gtggcactca
    42061 gaatcccttc cgagcagtct gatgcggtca ctctttgtca gggtcctcat agcgtttttt
    42121 cgcatccatc gcttcctgag ccgtcggatg ttcactgatc aacgagtctg gtttaggatg
    42181 atcggctctg agttggtacc aggttaccgt ctgtcctggc tttccttttt caatcgtcac
    42241 tatatatgct tcacgaggat aaggcggttt cgttggcatc gttcttcctt atttcacggg
    42301 atgaacgtta agtataggcg ctctaaaatc aacaattgat cgtctgtgcc agggcgctac
    42361 gaatttcaga aatcacctgg ctgggttcgt ttgttgcgtc gatgataata tgcgcaactt
    42421 cgcgatatag cgcatcgcgt tcttccagca cttcctgaac ttcttcgctc agcggttttc
    42481 ccgttaaggt tggccgtaaa tcttcttccg gtgcagcttg cagtcggtta accaggactg
    42541 atactggcgc acacaaataa accacgatcc cgttattttg catgaagtga cgattaaatt
    42601 ccgtcagaat aatgccgccg cctgtagcga taacggtgga tgccgcagtt accgcttcca
    42661 gcgccgccgt ttctctggcg cgaaatcccg cccactcttc cctttcgacg atctccgcga
    42721 ccgtcatatt gagctgtgat tgcaaccact gatcggtatc gacaaaccga cggttaagcg
    42781 aatcggcaag ggccattccg accgttgttt taccacagcc ccgaggcccg atcagaaaaa
    42841 gaggttgtgt catcgtgggt tttccccaat aggtcgcaat gcggcgaaag ccggtgtcat
    42901 gagaatagcg atcataccat caaactagta caatttcgat tgtaaagaaa aaattccact
    42961 taaagtaaaa atctcaatac atcccttact ataccaataa atattcaaga atgaagtgta
    43021 aataataaat tacatttagc cacgactacg ttgcacttcc agccaccact tctccagctc
    43081 cgcggcaaag gcctggcggt cacgttgtga aaggctatct ggtccgccgg tctggatccc
    43141 actggcacgt aaggtatcca taaaatcgcg catcgtcagg cgctcacgaa tggtcgctgg
    43201 cgtgtaacgt tcgccgcgcg aattaagcgc cgctgcgcct ttctcgatgg cttcagcagc
    43261 caaaggtata tctgcggtga tcaccaaatc gcccgcttca cactgccgga caatttcgtt
    43321 atcggcaacg tcgaaacctg ccgcgacgcg cagcgtacga ataaatcgcg atggcggcac
    43381 gcgtaaactc tggtttgcta ccagtaccag cggcatctgc atacgttccg ccgcgcgata
    43441 caaaatctct ttaattacat tgggacacgc gtcggcatcc acccaaattg tcataaagtc
    43501 atcctttgtt aggtaatcct ctattgtgtc gcgcttttgc cttccggcat agttctgttt
    43561 atgcttctgc cagcgattat caaaacaatg aatttcacgg caggagtgag gcaatggaaa
    43621 agaaaatcgg ttttattggc tgcggcaata tgggaaaagc cattctcggc ggtctgattg
    43681 ccagcggtca ggtgcttcca gggcaaatct gggtatacac cccctccccg gataaagtcg
    43741 ccgccctgca tgaccagttc ggcatcaacg ccgcagaatc ggcgcaagaa gtggcgcaaa
    43801 tcgccgacat catttttgct gccgttaaac ctggcatcat gattaaagtg cttagcgaaa
    43861 tcacctccag cctgaataaa gactctctgg tcgtttctat tgctgcaggt gtcacgctcg
    43921 accagcttgc ccgcgcgctg ggccatgacc ggaaaattat ccgcgccatg ccgaacactc
    43981 ccgcgctggt taatgccggg atgacctccg taacgccaaa cgcgctggta accccagaag
    44041 ataccgctga tgtgctgaat attttccgct gctttggcga agcggaagta attgctgagc
    44101 cgatgatcca cccggtggtc ggtgtgagcg gttcttcgcc agcctacgta tttatgttta
    44161 tcgaagcgat ggccgacgcc gccgtgctgg gcgggatgcc acgcgcccag gcgtataaat
    44221 ttgccgctca ggcggtaatg ggttccgcaa aaatggtgct ggaaacggga gaacatccgg
    44281 gggcactgaa agatatggtc tgctcaccgg gaggcaccac cattgaagcg gtacgcgtac
    44341 tggaagagaa aggcttccgt gccgcagtga tcgaagcgat gacgaagtgt atggaaaaat
    44401 cagaaaaact cagcaaatcc tgatgacttt cgccggacgt caggccgcca cttcggtgcg
    44461 gttacgtccg gctttctttg ctttgtaaag cgccaaatct gccgatttca accactcacg
    44521 atagtgactc atttgtgggt tcagcggcgc aacccccaca ctaatccgta aagttacctg
    44581 tggcgtattc ggcaagcgta acgtgtttag cccttcacgc acccgtaaca tggcggtaat
    44641 ggcgctctca gctggcgtac cggacatgat tactgcaaac tcatcgccgc caaaccgacc
    44701 aatcacatcg ctaccgcgca gggtaatttg taactgtcgg gtaagcgcca caatcgcttc
    44761 atcgcccaca tcatgacccc aggtatcgtt gatgctcttg aaatggtcga tatcgataat
    44821 cagtaacgtt gcatcgcgat tatgccgccg acagttatca aattcattgc gtaacatagt
    44881 ttcccaatga cgtcggttat acacgcccgt catgccgtcg cgagtactca tgacctgcaa
    44941 cctgcgttta tgttccgcca gtttggttgc cgtctggtag ctgacccagc caaacatcag
    45001 aggataaacg acaataatgg gaagggagag ccaccattcc agcggagcac tattgaacga
    45061 cacggtaatg cccgtaagct cgagggtgac aaggcaggaa accaccatca acaccagacc
    45121 cgcgacaaac agacgggggc cgcctgcccc catcaaattc agacacagaa tcatcaacat
    45181 agcggtggaa ggcagcacgt ttacgcccat tacgcctacc cacattcccg ctaatactgc
    45241 atcggttttt aagttgtaaa tttcccggct aagcggatcg acggccctgc tcgctatctg
    45301 ccaggctaaa tgcggccaga cgaacgccca gccgaccaac accagccacc accagcccgg
    45361 cggcgggtgc gaaaccagcg ttgaagcaat cggtaagaac atgccagcca ggccaaccgc
    45421 acggggtagt ctgacgcgac gggcgaagcg cagcccggac cgctgatgtt cgttttgagg
    45481 agttaaagga ggttcctccc catgcgccgc cgcttttttg aaaaagtttt catcattcat
    45541 tatttttggg aacattcaga aacaattttc ccaaattata gagacggggc taaaggcaga
    45601 agtatgaaat ttcgggtgag ccactttcgt agctcacccg ttagcact
//
LOCUS       DAJTUP010000023        43135 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTUP010000023 DAJTUP010000000
VERSION     DAJTUP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.023"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            52..1485
                     /gene="cscA"
                     /locus_tag="OO468_001302"
     CDS             52..1485
                     /gene="cscA"
                     /locus_tag="OO468_001302"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR6258484.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1493..2551)
                     /locus_tag="OO468_001303"
     CDS             complement(1493..2551)
                     /locus_tag="OO468_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6258485.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2719..2937
                     /locus_tag="OO468_001304"
                     /pseudo
     CDS             2719..2937
                     /locus_tag="OO468_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            2955..4283
                     /gene="dsdA"
                     /locus_tag="OO468_001305"
     CDS             2955..4283
                     /gene="dsdA"
                     /locus_tag="OO468_001305"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR6258486.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4391..5929)
                     /gene="emrY"
                     /locus_tag="OO468_001306"
     CDS             complement(4391..5929)
                     /gene="emrY"
                     /locus_tag="OO468_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR6258487.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(5929..7092)
                     /gene="emrK"
                     /locus_tag="OO468_001307"
     CDS             complement(5929..7092)
                     /gene="emrK"
                     /locus_tag="OO468_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR6258488.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7508..8122
                     /gene="evgA"
                     /locus_tag="OO468_001308"
     CDS             7508..8122
                     /gene="evgA"
                     /locus_tag="OO468_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR6258489.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8127..11720
                     /gene="evgS"
                     /locus_tag="OO468_001309"
     CDS             8127..11720
                     /gene="evgS"
                     /locus_tag="OO468_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR6258490.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11776..12921)
                     /gene="yfdE"
                     /locus_tag="OO468_001310"
     CDS             complement(11776..12921)
                     /gene="yfdE"
                     /locus_tag="OO468_001310"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR6258491.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(12995..13939)
                     /gene="yfdV"
                     /locus_tag="OO468_001311"
     CDS             complement(12995..13939)
                     /gene="yfdV"
                     /locus_tag="OO468_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR6258492.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14009..15703)
                     /gene="oxc"
                     /locus_tag="OO468_001312"
     CDS             complement(14009..15703)
                     /gene="oxc"
                     /locus_tag="OO468_001312"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR6258493.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15757..17007)
                     /gene="frc"
                     /locus_tag="OO468_001313"
     CDS             complement(15757..17007)
                     /gene="frc"
                     /locus_tag="OO468_001313"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR6258494.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17520..18143)
                     /locus_tag="OO468_001314"
     CDS             complement(17520..18143)
                     /locus_tag="OO468_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR6258495.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18440..18715
                     /locus_tag="OO468_001315"
     CDS             18440..18715
                     /locus_tag="OO468_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258496.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18792..19034)
                     /locus_tag="OO468_001316"
     CDS             complement(18792..19034)
                     /locus_tag="OO468_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR6258497.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19387..20307
                     /gene="lpxP"
                     /locus_tag="OO468_001317"
     CDS             19387..20307
                     /gene="lpxP"
                     /locus_tag="OO468_001317"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR6258498.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20662..20733
                     /gene="ypdK"
                     /locus_tag="OO468_001318"
     CDS             20662..20733
                     /gene="ypdK"
                     /locus_tag="OO468_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR6258499.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20798..22036)
                     /gene="alaC"
                     /locus_tag="OO468_001319"
     CDS             complement(20798..22036)
                     /gene="alaC"
                     /locus_tag="OO468_001319"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR6258500.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22413..23899
                     /locus_tag="OO468_001320"
                     /pseudo
     CDS             22413..23899
                     /locus_tag="OO468_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            23914..24648
                     /gene="ypdB"
                     /locus_tag="OO468_001321"
     CDS             23914..24648
                     /gene="ypdB"
                     /locus_tag="OO468_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR6258501.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24661..25518
                     /locus_tag="OO468_001322"
                     /pseudo
     CDS             24661..25518
                     /locus_tag="OO468_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25521..28016)
                     /gene="ptsP"
                     /locus_tag="OO468_001323"
     CDS             complement(25521..28016)
                     /gene="ptsP"
                     /locus_tag="OO468_001323"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR6258502.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28041..29078)
                     /gene="ypdE"
                     /locus_tag="OO468_001324"
     CDS             complement(28041..29078)
                     /gene="ypdE"
                     /locus_tag="OO468_001324"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6258503.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29078..30161)
                     /gene="ypdF"
                     /locus_tag="OO468_001325"
                     /pseudo
     CDS             complement(29078..30161)
                     /gene="ypdF"
                     /locus_tag="OO468_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30176..31423)
                     /locus_tag="OO468_001326"
     CDS             complement(30176..31423)
                     /locus_tag="OO468_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR6258504.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31445..31771)
                     /locus_tag="OO468_001327"
     CDS             complement(31445..31771)
                     /locus_tag="OO468_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR6258505.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(31990..32955)
                     /gene="glk"
                     /locus_tag="OO468_001328"
     CDS             complement(31990..32955)
                     /gene="glk"
                     /locus_tag="OO468_001328"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR6258506.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33159..34415
                     /locus_tag="OO468_001329"
     CDS             33159..34415
                     /locus_tag="OO468_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR6258507.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34530..34856
                     /locus_tag="OO468_001330"
     CDS             34530..34856
                     /locus_tag="OO468_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR6258508.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(34997..36235)
                     /locus_tag="OO468_001331"
     CDS             complement(34997..36235)
                     /locus_tag="OO468_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR6258509.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36571..37773
                     /gene="nupC"
                     /locus_tag="OO468_001332"
     CDS             36571..37773
                     /gene="nupC"
                     /locus_tag="OO468_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR6258510.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37823..39935)
                     /locus_tag="OO468_001333"
                     /pseudo
     CDS             complement(37823..39935)
                     /locus_tag="OO468_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40143..40218)
                     /locus_tag="OO468_001334"
     tRNA            complement(40143..40218)
                     /locus_tag="OO468_001334"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40183..40185),aa:Ala,seq:ggc)
     gene            complement(40258..40333)
                     /locus_tag="OO468_001335"
     tRNA            complement(40258..40333)
                     /locus_tag="OO468_001335"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40298..40300),aa:Ala,seq:ggc)
     gene            40554..40913
                     /locus_tag="OO468_001336"
     CDS             40554..40913
                     /locus_tag="OO468_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6258511.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40915..41307
                     /locus_tag="OO468_001337"
     CDS             40915..41307
                     /locus_tag="OO468_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR6258512.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41359..42774)
                     /gene="gltX"
                     /locus_tag="OO468_001338"
     CDS             complement(41359..42774)
                     /gene="gltX"
                     /locus_tag="OO468_001338"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR6258513.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43033..43108
                     /locus_tag="OO468_001339"
     tRNA            43033..43108
                     /locus_tag="OO468_001339"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43066..43068,aa:Val,seq:tac)
BASE COUNT        11473 a        10752 c        10014 g        10896 t
ORIGIN      
        1 taaaagttaa cgttaataat tcaccaaatt tgcttaacca ggatgattaa aatgacgcaa
       61 tctcgattgc atgcggcgca aaacgcacta gcaaaacttc atgagcaccg gggtaacact
      121 ttctatcccc attttcacct cgcgcctcct gccggatgga tgaacgatcc aaacggcctg
      181 atctggttta acgatcgtta tcacgcgttt tatcaacatc acccgatgag cgaacactgg
      241 ggaccaatgc actggggaca tgccaccagt gacgatatga tccactggca gcatgagcct
      301 attgcgctag cgctaggaga cgataatgac aaagacgggt gtttttcagg tagtgctgtc
      361 gatgacaatg gtgtcctctc acttatctac accggacacg tctggctcga tggtgcaggt
      421 aatgacgatg caattcgcga agtacaatgt ctggctacca gtcgggatgg tattcatttc
      481 gagaaacagg gtgtgatcct cactccacca gaaggaatca tgcacttccg cgatcctaaa
      541 gtgtggcgtg aagccgacac atggtggatg gtagtcgggg cgaaagatcc aggcaacacg
      601 gggcagatcc tgctttatcg cggcagttca ttgcgtgaat gggccttcga tcgcgtactg
      661 gcccacgctg atgcgggtga aagctatatg tgggaatgtc cggacttttt cagccttggc
      721 gatcagcatt atctgatgtt ttccccgcag ggaatgaatg ccgagggata cagttaccga
      781 aatcgctttc aaagtggcgt aatacccgga atgtggtcgc caggacgact ttttgcacaa
      841 tccgggcatt ttactgaact tgataacggg catgactttt atgcaccaca aagcttttta
      901 gcgaaggatg gtcggcgtat tgttatcggc tggatggata tgtgggaatc gccaatgccc
      961 tcaaaacgtg aaggatgggc aggctgcatg acgctggcgc gcgagctatc agagagcaat
     1021 ggcaaacttc tacaacgccc ggttcacgaa gctgagtcgt tacgccagca gcatcaatct
     1081 gtctctcccc gcagaatcag caataaatat gttttgcagg aaaacgcgca agcagttgag
     1141 attcagttgc agtgggcgct gaagaacagt gatgccgaac attacggatt acagctcggc
     1201 actggaatgc ggctgtatat tgataaccaa tctgagcgac ttattttgtg gcggtattac
     1261 ccacacgaga atttagacgg ctaccgtagt attcccctcc cgcagcgtga cacgctcgcc
     1321 ctaaggatat ttatcgatac atcatccgtg gaagtattta ttaacgacgg ggaagcggtg
     1381 atgagtagtc gaatctatcc gcagccagaa gaacgggaac tgtcgcttta tgcctcccac
     1441 ggagtggctg tgctgcaaca tggagcactc tggctactgg gttaacataa tatcaggtgg
     1501 aacaacggat caacagcggg caagggatcc gcgtcactct tcccccttca cgaccttcaa
     1561 taatatgcaa tgcagcttcc cgcccgataa tgtcatgtgg aagctgaatt gtggtcagcg
     1621 gcggtaaaaa cagatgcccg acgccaacca gattatcaaa gcccattaca gcgacatcct
     1681 gcgggatacg tacccccttc gccaaaagaa cctgataagc cacaaaggct gcgcgatcgt
     1741 taccacatat cagaacatca aaatctggtt tgcccgattt gaagtgagca ttgagtaaac
     1801 ttgcgagatc ggtgtagtga tcatcacctg ttgccatgtg aaattgtttc acctcagcca
     1861 gatctcgtcc agcatcacgc caagcctgct caaatccctg ccgacgatac cctgttgcca
     1921 acgcactttc cggtagccag aagcataatg gttgacgata gcccgccgcg agcaaatgct
     1981 gtgttgattc atattgtgca gtgtaatcat cagggatata actgggtaac gctgggtcat
     2041 ccgccacaca gttcgccaat acaatatttt caccatacag agactcaggc agcgtgatat
     2101 gtcgcagccc cattgtagta tagataatgc catccggacg gtgggcaagc agctgacgtg
     2161 ccgcgcgggc agcgtcatct tcagaaaaaa tattgattaa aaaactattc cagccgaact
     2221 cgctggcggt ttgctcaatg gcaagcagaa tatcaacaga gaaaggagtg gtagccgtgt
     2281 cctgcgccag cacggcgaga gtcgacggct tacgtccttg agcgcgcatc ttacgggcgg
     2341 aaagatcagg aacataattc agggtctgga ttgcctgcaa tacgcggtca cgcgttgcag
     2401 gacgcacaga ttctgcatta tgcatcaccc gggagactgt catcatcgac actcccgcca
     2461 ggcgtgcgac atcctttaat gaagccatac ccaagccgtt tgccgtaaaa cgggcactgt
     2521 agcagaaaca gacgtcactg gcgagatcca acgccctatc acctgacaca gcaatacaat
     2581 aaaaaataac aataattccc ggacaattgt ccccaattcc gcctctgttc tcgccaacga
     2641 gtaaccatat aggcaagtaa gtaaaacatg ccaactggca tcgccaacct ggagaagaaa
     2701 atataaatat ggatcattgt gctgccactg tatcccgaca tcagcccgga aattattgcg
     2761 attgctatcg gttcaggtgc aattggctgc acgatcgtta cggactcgct tttctggctg
     2821 gtgaagcaat attgcggcgc tacgctcaat gaaacattta aatactatac gacagcgaca
     2881 tttatcgctt cagtcgtcgc tctggcgggc acattcctgc tgtcatttat catctaagcg
     2941 caaagagacg tactatggaa aacgctaaaa tgaactcgct catcgcccag tatccgttgg
     3001 taaaggatct ggttgctctt aaagaaacca cctggtttaa tcctagcacg acctcattgg
     3061 ctgaaggttt accttatgtt ggcctgaccg aacaggatgt tcaggacgcc catgcgcgct
     3121 tatcccgttt tgcaccctat ctggcaaaag catttgctga aacagctgcc actgggggga
     3181 ttattgaatc agaactggtt gccattccgg ctatgcaaaa acggctggaa aaggaatatc
     3241 aacaaccgat cagcgggcaa ctcctactga aaaaagatag ccatttgccc atttccggct
     3301 ctataaaagc acgcggcggg atttatgaag tcctggcaca tgcagaaaaa ctggctctgg
     3361 aagcggggtt gctgacgctt gatgatgact acagcaaact gctttctccg gagtttaaac
     3421 agttctttag ccaatacagc attgctgtgg gctcaaccgg aaatctgggg ttatcaatcg
     3481 gcattatgag cgcccgcatt ggctttaagg tgacagttca tatgtctgct gatgcccggg
     3541 catggaaaaa agcgaaactg cgcagccatg gcgttacggt cgtggaatat gagcaagatt
     3601 atggtgttgc cgtcgaggaa ggacgtaaag cagcgcagtc tgacccgaac tgtttcttta
     3661 ttgatgacga aaattcccgc acgttgttcc ttgggtattc cgttgctggc cagcgtctta
     3721 aagcgcaatt tgcccagcaa ggccgtatcg tcgatgctga taaccctctg tttgtctatc
     3781 tgccgtgtgg tgttggcggt ggtcctggtg gcgtcgcatt cgggcttaaa ctggcgtttg
     3841 gcgatcatgt tcactgcttt tttgccgaac caacgcactc cccttgtatg ttgttaggcg
     3901 tccatacagg attacacgat cagatttctg ttcaggatat tggtatcgac aaccttaccg
     3961 cagcggatgg ccttgcagtt ggtcgcgcat catgctttgt cgggcgggca atggagcgtc
     4021 tgctggatgg cttctatacc cttagcgatc aaaccatgta tgacatgctt ggctggctgg
     4081 cgcaggaaga aggtattcgt cttgaacctt cagcactggc gggtatggcc ggacctcagc
     4141 gcgtgtgtgc atcagtaagt taccaacaga tgcacggttt cagcgcagaa caattgcgta
     4201 atgccactca tctggtgtgg gcgacgggag gtggaatggt gccggaagaa gagatgaatc
     4261 aatatctggc aaaaggccgt taataacgtt tcaacgcaac atcgcaatcc tttccctggg
     4321 tgagcgatgc tgccgatggc gcagacttaa gatccccggt cttacccgct ataaccccct
     4381 ccttttatca tcacccaacg cctttcgctg taaacggcgg tttcgcaaac caaaccaaaa
     4441 ccgtaagtaa gataaaagca atagccgcca tacggaaaat ctcatttgca gaaatagaaa
     4501 gtgactgctg ggttatttca ttattaattt cattaagaac tcctgaaagc gaaccatagt
     4561 atttatccat aatttgtgac gatgaattaa acacggggtt aaattgatcg atggttgctg
     4621 tcaactgact atggtgtaac gattcgcgtc gtccccacag cgtcattgtc aacgacgtac
     4681 caactgatcc tgacaaggta cgaaaaaaat tactcatact cgaggcattg gcaaatttat
     4741 tatctggcaa gcctgaaaac gaaatcgttg ttaagggtaa aaagaaacag gcaacggcga
     4801 atccctggaa aaactgtggc aaaatgatgc ctgtaaaatc aatcgttggc ataaatgtca
     4861 cagaacgcca gtaatagcaa accgcataca tcaaaaaact gaatgtcact aacacccgca
     4921 tgtctatttt attgccataa cgtcctatca aaggtgaaat caatagtggc atgatgccga
     4981 tgggcgcata agcaagcccg gcccatatcg cattataccc catcgttttc tggagtaact
     5041 gcggcataag gacgatcgct ccagagtaaa ataaatacgc gcaggtgata ctcacaatac
     5101 caatggtgaa attacgggac ttaaacaaac tgagatcaag aatcgggttc tctgaggtcg
     5161 attcccaaat gactaaagag atcagagaaa taactgatac tactgttaat attattattg
     5221 tactcgagtt gaaccaatcc agatcgcgcc ctttatcaag cataatttgc aagccaccaa
     5281 caccgagcac taacagggtc agtcttggta gattcatttt taccggtgaa gtttcagttt
     5341 ctcttccttt aagtaaggtt aagcataatg tcaggacgat aatccccata gggacattga
     5401 ttaaaaatat ccaaccccag ctaaagttat cgcaaatata gccgcccaat atcggcccac
     5461 atatcggagc gataatcacg gtcattgacc ataatgccag agcaaatgtt cttttttctg
     5521 gtggataatt ccttaataat aaactctgtg acagtggaat taacggcccc gccattaatc
     5581 cctgaacgac tctaaaaaat atcagcacat caagattggt cgataaacta cacattaagg
     5641 aagacagcga aaaaaaagtg actgaaagta aaaataacct taattcgcct attctttgtg
     5701 ccagcctgcc ggtaacaggg atcgcaatgg catttgctac gccaaacgag gtgataaccc
     5761 aggtgccttc gtctgttgat gctcccagaa agccagatat tgtcggtatt gcgacgttag
     5821 aaatagtgga atccaacatt tgcataaacg tcgctaatga caatgcaata gtaacgcacc
     5881 ataacgtccc accggttaat ggtgccggag ttgatttagt gattgccatt aaagttgtcc
     5941 attatgcgaa ataatgttgc taatttcttt ttctatcgga ctggtatcga taactaaagc
     6001 cttactggta taagcaggca tggaggtcac ggttgaagcc agatcaggca tctcagcaat
     6061 gtcttcgtcc ttcgtatcaa tagttgcagt catcgataaa ccaatgcgca aggggtgttc
     6121 catgagttct tttggatcaa gagaaacttc aaccggtaca cgctgaacga ttttgatcca
     6181 gttccctgtc gcattttgtg caggtaataa ggagaacgca ttgccggttc ccatattgat
     6241 ccctgtcacc cgaccatgaa acacaacatt ttcaccataa agatcgctga taatattgac
     6301 tgattgacca atccgtacat ccgtgagttg tgtttcttta aagttggcat taacccacat
     6361 ttgacgtgcc ggtacgacag ccattaacga ttgtccggaa ctcactgttt cgccgacctg
     6421 aacacttcgc tgggcaatat agccagtaac cggactcctt atatccgtac gtttaagcac
     6481 caaccaggct tctttggttg catccgccgc ttcaactact tgtggctgac ggtttaatgg
     6541 tgtgttcatt actaaagctt tattcgcttt ataagcctgg atagcggcat tcagtgccgc
     6601 tttgctactt attaacgtat ctttggtatg ctccagcgtt tcttttgaaa taaccccctg
     6661 cttcgctaaa ggcacacgac ggttataatc ttctaaagat tgttgatact gaatacgtgc
     6721 tgaagcgact tcggcactgt attgtttatc ctgtaagtac agtttattcg tttgccgaac
     6781 aatatttgcc agattatttt tagctttatt gagtgcgata gtggcatcag ttttatccag
     6841 tgaaactaaa atgtcaccct gtcgaacgta gttcgtatct ttatgattaa ctacagtgac
     6901 actacctgag acttgtgcag aaattggatc tgcattcccc gtgacatagg cgtcatctgt
     6961 actaatcatg tcttctaatt ccattgacca ataggcatag gcacctgaaa acgcaataaa
     7021 taaaactatc accaataaag aaaagtattt ttttctgtta gactgttttt tatttgaatt
     7081 aatctgttcc actattatct ctcatttcgc atagatgaaa tttatgaatt gaatgaaaag
     7141 agtattcatt cttcatatca aacacagtgc atgtgttgca cattatccca ggaacgttgg
     7201 aggcctaatt taaatgaggt gcaaaaaaaa caacagagga ctaaaccgtg gcttttgcaa
     7261 tacaattctt acgcctgtag gattagtgag aagacttata gtgctaactt gaaactataa
     7321 atcatcggta taatccctga ttttattgtt gacatttcat ttatgccgac tatttatatg
     7381 gtatacttgt cgaattatct taaaggaaac tcagattttc ttatttttat tgagaaaatg
     7441 agatgacgcc ttatgtctgt attactacag ggagaaggga gatgcttcat tgcaaaggga
     7501 ataatctatg aacgcaataa ttattgatga ccatcctctt gctatcgcag caattcgtaa
     7561 tttattgatc aaaaacgata ttgaaatctt agcggagtta actgaaggcg gaagcgccgt
     7621 tcagcgggtg gaaacactta agcctgatat cgtcatcatt gatgtcgata tccccggagt
     7681 taacggtatc caggtgttag aaacgctgag gaagcgccaa tatagcggaa ttattattat
     7741 cgtctccgct aaaaatgacc atttttacgg gaaacattgt gctgatgctg gcgctaatgg
     7801 tttcgtgagt aaaaaagaag gcatgaacaa tatcattgcg gctattgaag ctgcaaaaaa
     7861 tggctactgc tatttcccct tctctctcaa ccggtttgtt ggaagtttaa cgtccgacca
     7921 gcaaaaactc gactccttat cgaaacaaga aattagtgtc atgcggtata ttcttgatgg
     7981 caaggataat aatgacattg cggaaaaaat gttcatcagc aacaaaactg tcagcacgta
     8041 taaaagtcgc ctgatggaaa aattagaatg taaatcactg atggatcttt acacattcgc
     8101 gcaacgtaac aaaatcggct aatcacatga agtttttacc ctattttttt cttctctgtt
     8161 gtggtctttg gtcgaccata agtttcgcag acgaagatta catcgaatat cgtggcatca
     8221 gtagtaacaa ccgtgtcaca cttgatccag tacgtctgag caacaaggaa ttacgttggt
     8281 tagcgagcaa aaaaaatctt gtgattgcag tacacaagtc ccaaacggct accttgttgc
     8341 ataccgattc gcagcaacag gttcgtggta ttaatgctga ttatttaaat cttttaaaaa
     8401 gagcgttaaa tatcaaatta acactccggg aatacgcaga tcatcaaaaa gcaatggacg
     8461 cgcttgcaga aggtgaagtc gatatagtgt tatcacattt agttacttcg ccgcctctta
     8521 ataatgacat tgctgcaacc aaaccattga taattacctt tccggcgctg gtaaccaccc
     8581 ttcacgactc aatgcgaccg cttacctcac caaaaccagt aaatattgct cgggtagcaa
     8641 attacccccc agacgaggta attcatcaat catttccaaa agcaacaatt atctctttta
     8701 caaatttata tcaggcatta gcatccgtct cagctgggca caatgattac tttattggta
     8761 gtaacatcat taccagcagt atgatttccc gctatttcac tcactcctta aatgtagtga
     8821 aatattataa ctcgccgcgt caatataatt ttttcttgac cagaaaagaa tctgtcattc
     8881 ttaatgaagt actcaataga tttgttgatg ctttaacaaa tgaagttcgc tatgaagtat
     8941 cacaaaattg gcttgataca ggaaacctgg cctttctgaa caaaccatta gaactcactg
     9001 aacatgaaaa acagtggatt aagcagcatc ccaatttaaa ggtgctggaa aatccttact
     9061 cgccccccta ttctatgacg gatgaaaatg gctcggttcg gggcgttatg ggggacattc
     9121 ttaatattat taccttgcaa acaggtttaa atttttctcc gatcaccgtt tcacacaata
     9181 tccatgctgg aacacaactt agccccggag gatgggatat aatacctggc gctatttata
     9241 gtgaagatcg agaaaataat gttttatttg ctgaagcctt cataacaacg ccttacgttt
     9301 ttgtcatgca aaaagcgcct gacagtgaac aaacattaaa aaaaggaatg aaagttgcca
     9361 ttccatatta ttatgagctg cattcgcaat taaaagagat gtatccggag gttgaatgga
     9421 tacaggtcga taatgccagc gctgcatttc acaagattaa ggaaggtgaa cttgatgctc
     9481 tggtcgcgac acagctaaat tcgcgttaca tgatcgatca ttactatcct aatgaacttt
     9541 atcattttct tattcctggc gttccgaatg catcgctttc gttcgctttt cctcgcggag
     9601 aaccggaact taaggatatt attaataaag cactgaatgc aattccccca agcgaagttc
     9661 tgcgcctgac ggaaaaatgg attaaaatgc ccaatgtgac cattgacaca tgggacctat
     9721 atagcgagca attttatatt gttacgacat tatccgtttt attagttggc agtagccttt
     9781 tatggggatt ctacctgtta cgctcagttc gtcgtcgtaa agtcattcag ggtgatttag
     9841 aaaaccaaat atcattccga aaagcactct cggattcctt accgaatcca acttatgttg
     9901 taaactggca aggtaatgtc attagtcata atagtgcttt tgaacattat ttcactgcgg
     9961 attactacaa aaatgcaatg ttaccattag aaaacagtga ttcacccttt aaagatgttt
    10021 tttctaatgc gcatgaagtc acagcagaaa cgaaagaaaa tcgaacaata tacacacagg
    10081 tatttgaaat tgataatggc atcgagaaaa gatgcattaa tcactggcat acattatgca
    10141 atcttcctgc aagtgacaat gcagtatata tttgtggttg gcaagatatt actgaaacgc
    10201 gtgatctaat taatgcactc gaggtagaaa aaaataaagc gataaaggct accgtagcaa
    10261 aaagtcagtt tctggcaacg atgagtcacg aaataagaac accaataagc tctattatgg
    10321 gcttcctgga acttctgtcg ggttctggtc ttagcaagga gcaacgggtg gaggcgattt
    10381 cacttgccta cgccaccgga caatcactcc tcggcttaat tggtgaaatc cttgatgtcg
    10441 acaaaattga atcgggtaac tatcaacttc aaccacaatg ggtcgatatc cctactttag
    10501 tccagaacac ttgtcactct ttcggtgcga ttgctgcaag caaatcgatc gcattaagtt
    10561 gcagcagtac atttcctgat cattacctgg ttaagatcga ccctcaggcg tttaagcagg
    10621 tcttatcaaa tttactgagt aatgctctca aatttaccac cgagggggca gtaaaaatta
    10681 cgacctccct gggtcacatt gatgacaacc acgctgttat caaaatgacg attatggatt
    10741 ctggaagtgg attatcgcag gaagaacaac aacaactgtt taagcgctac agccaaacaa
    10801 gtgcaggtcg tcagcaaaca ggttctggtt taggcttaat gatctgcaaa gaattaatta
    10861 aaaatatgca gggcgattta tcattagaaa gtcatccagg cataggaaca acatttacga
    10921 tcacaatccc ggtagaaatt agccagcaag tggcgactgt cgaggcaaaa gcagaacaac
    10981 ccatcacact acctgaaaag ttgagcatat taatcgcgga tgatcatccg accaacaggc
    11041 tattactcaa acgccagcta aatctattag gatatgatgt tgatgaagcc actgatggtg
    11101 tgcaagcgct acacaaagtc agtatgcaac attatgatct gcttattact gacgttaata
    11161 tgccgaatat ggatggtttt gagttgactc gcaaactccg tgagcaaaat tcttccttac
    11221 ccatctgggg gcttacagcc aacgcacagg ctaacgaacg tgaaaaaggg ttaagttgcg
    11281 gcatgaactt atgtttgttc aaaccgttga ccctggatgt actgaaaaca catttaagtc
    11341 agttacacca ggttgcgcat attgcacctc agtatcgcca ccttgatatc gaagccctga
    11401 aaaataatac ggcgaatgat ctacaactga tgcaggagat tctcatgact ttccagcatg
    11461 aaacgcataa agatctaccc gctgcgtttc atgcactaga agctggcgat aacagaactt
    11521 tccatcagtg tattcatcgc atccacggtg cggctaacat cctgaatttg caaaagttga
    11581 ttaatattag ccatcagtta gaaataacac ctgcttcaga tgacagtaag cctgaaattc
    11641 ttcagctgct gaactctgtt aaagaacaca ttgcagagct ggaccaggag attgctgttt
    11701 tctgtcagaa aaatgactaa attgcggctc ccacaatgtt ctaatgtggg agctatttac
    11761 cagcacatct tacatttatg atgagaactc ctggcgaatt tgttccccat gctggtcgag
    11821 cgttgccgct cccggcataa catgcgggtc cgcgcagccg ctgattttta tcggattacc
    11881 cggcatcatt attcctccgg cttcaatcaa catatttctc gcctgagttt gtggcaaatt
    11941 aatggcctca gccacactta ataacggcgc gacgggcaca ccaacttcat gtattttggc
    12001 taaccaaact tcagctgcct gcgtttttaa cgttcgctca atatattgtt taagaatagc
    12061 ctggttttgt acgcgtaaaa tattgctgct aaatcgggga tcattaacca gttccgtaag
    12121 ctccagtgcc tggcataacg cagaaaaaag cttgtcatta ccacaacaaa tcgtaatcgg
    12181 cttatcctga gtatcgaaaa catcaaaagg tgccatgtag ggatggcgat ttcccaggcg
    12241 ttgtggtgac ttccctgtcg caatatatgc catcagacca tgctccagaa aactcagcgt
    12301 ggcatcaaac atcgctatat cgacatgcgc ccctctctgg ctcttttcgc ggccataaag
    12361 tgcactcact attccgctga ataaatagac accgccgcat agatccgcaa gagaggtacc
    12421 aacacgcact ggcggagcat cagggtaccc tgtttccatc attatcccac tcattgcctg
    12481 aatgatggta tcgtaggcag gagcatcttt tagcggaccg gtatgtccga aacctgacga
    12541 tgaagcatat atgaggcgcg ggttgatttc ttgtaacctt tcccatgaaa accccagttt
    12601 ttccattgta cctaggcgaa aattctcagc taatacatca gcttgtttga gcatatttat
    12661 aaatatactt ttatcgtgat cattctttaa atcaagaacc acactctctt tgccatgatt
    12721 aataaaactg taatagagtg actgtccatc cacataggga ccaaatgtgc gggtatcatc
    12781 accatgacct ggtggctcaa ctttaattac ccttgcgccc atattataaa gaagttgagt
    12841 tccgaaaggt ccattaagga catgtgtcat atcgataact aataagcctt caaacggccc
    12901 tttgctttca ttatttgtca tttgcttatg ccttgaaaaa agccgaaggt gaaaagacac
    12961 ttcggcattt atgtaaaatc aataacatga ttaattatga aaccagtcga ctgacataaa
    13021 tccacaaggg agccgtgacg acaaagccca gtacgctcac agccaatgac gctgtaccag
    13081 tacgggtgta gacattaaac cggctggcaa taatgattcc ggagaatgcc ggcggtaatg
    13141 cgcctgccag taccatcatc tgcaaatgtt cgctgttcaa atgacatgcc ataccaacga
    13201 gaagcagcgc cagtggcatc agaatcaact tcaggaaggt gttataagca atttcggcac
    13261 tgaactcgaa tttatgtgca gccagagtca accccgcagc gaataccgct acccctgagt
    13321 tagccttcgc aatcagatta aaggttgggt cccatgctgc cggaattttt accccaacca
    13381 gcaccaggat cgttgccaga acaggtgccc atactactgg ctcctttgcc gcagaaatta
    13441 atgcgctcag attactattc ttcttaccat ccgctcctga ggaaggattc agcaaataca
    13501 gaccaatagg aatagtaatt gcgttaacaa taatagaaat aattgctacc actaaacctg
    13561 ttgatacgga atcaccataa ataggatcga gaactgcaaa ccccaggaat ccaatggtag
    13621 gtgaacccgc aattaatgca catacagctg cttctgcatg ggtacgttta aaaaatttgt
    13681 agcaaccaaa ccaggagaaa aagaaacatc cgacaataac cacaagtgat acaagggtca
    13741 gacgagtgtc cgcaaaaatc atttccctgt ttgcccgggt aatagaaaca aatagagccg
    13801 caggaagagc gtagtttaat accagtttat taaatgcgcg ggcttgatct tctgaaaaag
    13861 tttctcgtct gccgctaaaa taacccaaca gcatgattac gataataggc aataaatcgc
    13921 caataaaaaa tgttagcata atagtatttc cgataagggt cccctcacca tcgcataatg
    13981 agttggcaag aggagacgat gtcagtattt aattaccagc gacttgtttt gggttaagtt
    14041 tggtaatatg gccactttca gttcctgctg cagggtcgat aaccacatta ataatggtcg
    14101 gtttgcgcga ctgaataccg gtggttaaag catgacgaag ttcatctgtc gtggtgacgt
    14161 tatagccaac gccacgaaac gcatccatta atttgtcata ccttgcatgg tgcaacagat
    14221 ccgttggtga tggtgcacca gcgccactga gatcaacacc gtctcctctg tagatgccgc
    14281 cattattaaa aataacgatc gtcaccggca ggttatatcg acaaatcgtt tcaatttcca
    14341 tcccactgaa accaaaagca ctatcacctt caatggcgac aaccggagaa ccagaagtca
    14401 cgctagcacc gatggcatag cccataccga tgcccatgac accccaggta ccacaatcca
    14461 gacgacgacg tggtttatac atatcaataa tatttcgtgc attatccagg gtatttgcac
    14521 cttcattaac taaataaata tcctggttct cgcgcaatac gtcgcgcaca gcacttaatg
    14581 cattaaagta atttaatggt tgagtatctg tacttaattt ttcatgcatt ttttgtgcat
    14641 tttgctgctt gtggatattt aaaatatcgc gccataccag tggagtcgta aatgtgtttt
    14701 gtttcagttc tgccagcata ccttgcatac tggatgcaat atcaccaacg actggcacag
    14761 caatggggcg gttgctgtca atttcctgcg gttcaatatc cagttgaata aactgtgtat
    14821 ctgccgccca tcctttttta ccgtgtgcca ataaccaatt cagtcgtgca ccaacaagca
    14881 tgacaacgtc agcatttgcc agggcaaacg aacgcgcagc tgccgcagaa agtggatgcg
    14941 tatcttcaag gatccctttc gccatagaca ttggcaggaa tggaatctga gcactttcaa
    15001 taaattcacg aagctgttca tcagcttgtg aatacgccgc gcctttgcca aggataatta
    15061 atggccgttc agcttttgct aaaagcgaaa ttgcgctagt gactgacttc gggcatggca
    15121 ataatgctgg cgacggattt tcaactttaa caatcgtggt taacgcttcg tctttttcca
    15181 tcgtcgcggc caggacattt gctggcaaat caagataaac tccgccaggg cgacccgata
    15241 cagagacccg gatagcgcgt gccaatgcaa tgccaagatc ctgcggctga ttaacgcgaa
    15301 atgctgcttt ggcatacggt tttgccgcat tcatttggtc cagctcttca taatcacctt
    15361 gctgtaggtc gacgatcgcg cggtcgctgg agccgctaat catgataatc ggaaaaccat
    15421 ttaccgttgc gttggccaat gcggtcaaac cattgaggaa tcctggcgca gaaactgtca
    15481 ggcagatccc cggtttttgg gtaagaaaac cgcttgccgc agcggcatag cctgccgact
    15541 gctcatgacg aaaaccaata taacgaatgc cttccgcctg ggcatggcgt gccatatccg
    15601 tcacaggaat acctacaaca ccataaatag tgtcaatatt attctgtttt aatgcttcaa
    15661 cgatgatatg cataccatct gtcatttgaa gttgatctga catgattaat aacctcgaaa
    15721 tttttataaa ttgggagcca gaagcccccg ttaatatcag atggcgtggt tttgcttcat
    15781 tgcagcaatt tcatcgtcgc tataacccag ctcctgcaat acagcagcgg tatgttcacc
    15841 taatagcggc gcagctttaa tatccggcgt aaaggcagag aatttcattg gacaaccaac
    15901 ggtcagatat tttccacgca acggttgttc cacttcaaca acactgccac tttggcgcaa
    15961 agagggatca agtgaaattt ctttcatact taaaaccggt gcacaaggaa tatcaaactg
    16021 agtcaaatag gccaccgctt catgtttatc aatagtgaca gtgtattttt cgatttcagc
    16081 aaaaatatcg aaaatatgtg gctgtcgggc atgggctgta ctgtatgccg gatcggtaat
    16141 ccattctggt ttgccgatgg ctttacaggt gttttcccag ttttgctcct gaatagtgaa
    16201 ataaatatag gcgttaggat cggtttccca gcctttacat ttcaggatcc agccaggctg
    16261 accgccacca cctgcattac caccgcgggg aactgcatca ccaaatgtac cattcggata
    16321 ctgcgggtat tcttccagat aacccaattt atcgagacgc tgctggtcac gtaatttcac
    16381 gcggcaaagg ttcaatacgg catcctgcat tgacatggtg actcgttgcc cacgccccgt
    16441 tttttcgcga tgcagcaaag cagcaagtaa accgatcagc aaatgcattc cggtgttgct
    16501 gtcacccaac gctgcagcgc ttaccagcgg cggaccatcc caaaaacccg tagtggatgc
    16561 cgcgccaccc gctgcctgag caacgttttc ataggctttt acattcacat aaggcgaaca
    16621 ctcatcaaac cctttgatcg aaccaaaaat cagacgtgga ttgatttctt gaatatgctc
    16681 ccaggtgaag cccatgtgat caatggcccc tggatgaaag ttctcgacta agatatcagc
    16741 ttcgcggatc agcttttcca ttacctcttt gccttccgct gttttggtat ttaactcaat
    16801 agaacgtttg ttactgttaa gcatggtgaa gtaaagcgca tcgatatcag gaatatctcg
    16861 cagttggtga cgcgttacgt caccaacgcc gggacgttca attttaataa cgtcagcgcc
    16921 aaaccaggcc agcatttgag tacaagatgg gccagattgc acaccggtga aatcgagaac
    16981 tttaattcct tgaagtggag ttgacatagc aatacctatt agttaattaa atatgtgtta
    17041 atttagcgtt tatttaaggc ataacgccct ctataaataa tctgcaaaat atcttcgtag
    17101 cggcactcga tatttttaaa ttcctgtatc aagtactgaa aattatttta tcgccacttc
    17161 caattccttt ctgcatggta agtagtgagg acgaaaagaa gaatatgata aatattaatt
    17221 cgtggtaatc agagagagtc gcgacatttg tgatgttatt cttacaactg taagaagcgc
    17281 cggtgattta caagtaagag tatgattacg ctttttaaag cgtaccataa agcataacat
    17341 aataaatgat ttgtgatgac acgggattat tgcggtttac tttgaggaaa ataaaatgcg
    17401 tgtttataga accgcgagcg ccaaataatt ttatgaccac aacctgtcgt attgtggtca
    17461 taaaaaattg tcggctggag ggagttaaat cctttacctc aatatactgc ctgtcagcat
    17521 tagttcacaa acagtgcttc gctatcgacg atgattccat cttccgcacc tttaagcgcc
    17581 aggtttgcct cgtagtattg ctttttatca agcagtttct gcgcatcagc aagtgcatta
    17641 cgagtctgtt taagcggcat cagatattgg ttttccatta cgccaacacc ggccaggcga
    17701 agctcttcca ttgcgccctt tttatcccct ttcgccattt tttcattggc aatttttatc
    17761 gcggcttctt tttcgggtgt tgctacataa ctctcagaaa ttccgacaga tgcattaatg
    17821 acaatatatt ggtcatcatt gagattggtc tttttacctg gcttagcgaa ctttgcccat
    17881 tcggtactgt catctgacag caaagcggaa gcttcattgg tcagttcttt tgccttttcc
    17941 ggatcgccat gaaacagagc caggcgcgct acctgcacgt cacgcatcgc atataaacct
    18001 tgttcagaga tacgctctgc tgctgccgtt ttctgggttt gctgagtttg ctgttgtgcc
    18061 gctaccggtg cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc
    18121 gtagccataa ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc
    18181 catcatattg ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt
    18241 atcttacaat tgtagttatt atcgcaaagc ggcatttagt cttacagttg taggaatatg
    18301 cctcctgtcg cagagaacta ttcttatata ccacaaagag atgtcattta ttatattgat
    18361 gtaaatattg attaattata tgtaaataca gccataacat cgtatggttc tcagtgaacc
    18421 attttcagga taactttcta tgaaagtaaa cttaatactt ttcagcttat ttttattggt
    18481 ctctattatg gcatgcaatg tttttgcatt ttccatttct ggtggtgtaa gtgagaggag
    18541 ctataaagag actgaaaaaa catcagcgat gacgaccaca cactctacaa aacttcagcc
    18601 atcacaggcg attttgttta agatgagaga agatgtgcca ccattaaacc tcacagaaga
    18661 aatcacgcct acttatccga caaaggcgaa ttatcttatt catcctgtgc gatagcgtct
    18721 gtcacatggc aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc caaatgagcc
    18781 atcctgaccc actatcgcct gccatacccc accataaagc gaatcatcaa gccacaagcc
    18841 ggggcaagaa acataaatat ccccgtcacc agttcattac cgccagttaa ccacatggtg
    18901 aagtaataaa tgagtgcagc gaggatcacc atgccgaaga aagaagagac cgccgatacc
    18961 acgttcagta cctgaccgat gtagccgctt acgcagacaa tacacagggc gagaaacatc
    19021 cataaataaa tcatagctca acctcctgcc gcgctaaaaa acctgtaact tcagtcactt
    19081 taatagtaag acaatcctta acctctggca atttttgcta agcatcaatg aaaacagcac
    19141 gttaaatgca acgctattgc gtgcggaaaa ttttttactt tgcacgatta ataatcagta
    19201 gctgaaagca gtcagccagt gataaagtag agccaccgca ttcatgctcc tcgtggttat
    19261 gtcctgacta gtctttacac tctttacagg aaccattgtc gtacatgatg gcccaaccaa
    19321 ttgagtgtac cgctgtcgac atggaatctt ctgcgataca acaattcgta tctacagaag
    19381 gtaactatgt ttccacaatg caaattttcc cgcgagtttc tacatcctcg ctactggctc
    19441 acatggtttg ggcttggtgt actctggctt tgggtacagc ttccttatcc tgttctctgc
    19501 tttctcggca cgcgtattgg cgcaatggcg cgaccattcc tgaaacgtcg tgaatctatc
    19561 gcccgtaaaa acctggaact ttgtttcccg cagcattctg cggaagaacg cgagaagatg
    19621 attgccgaaa actttcgttc actcggcatg gcgctggtag aaaccggcat ggcatggttc
    19681 tggcccgaca gtcgcgtacg taaatggttt gatgttgaag ggttggataa ccttaaacgc
    19741 gcacaaatgc aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc gctggaactg
    19801 ggcggccgcg tgatgggact gtgccaacca atgatggcta cctatcgtcc acataataat
    19861 cagctgatgg aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc gatgatcggc
    19921 agaaataatc tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt atggtttgct
    19981 cccgatcagg attatggtcg taaaggcagc tccttcgcgc cgttctttgc ggtggaaaat
    20041 gtcgccacaa ccaatggcac ctatgttctc tcccgtctct ctggcgcagc catgttgacc
    20101 gtaacgatgg taagaaaagc ggattacagc ggatatcgtt tgttcatcac cccagagatg
    20161 gaaggctacc cgacagatga aaatcaagcc gctgcttata tgaacaagat tatcgagaaa
    20221 gagatcatgc gcgcaccgga gcagtacctc tggatccacc gtcgctttaa aacgcgcccg
    20281 gtgggagaat cgtcgttgta catttaacaa actgctctta ctctttccct cctcaaatcg
    20341 gccataataa cctcatgatt tttatagctt ttttcttaag ttttttcctt cgctgaatat
    20401 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20461 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta gcgccgtctc
    20521 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20581 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20641 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20701 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20761 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    20821 gtttgctgct gacgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    20881 cctgacgaat acggtcgtgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    20941 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21001 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21061 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacgac
    21121 ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21181 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21241 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21301 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21361 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21421 gcacatcgta gcatttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21481 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21541 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21601 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21661 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21721 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21781 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    21841 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    21901 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    21961 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22021 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22081 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22141 agaacatttt tatcaatgga tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22201 tacgctaaca agaaaatcac caccaatttt attaacatta taatttcaat aaatttacca
    22261 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt taatcccctt
    22321 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22381 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22441 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22501 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22561 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22621 tggtgaacgt acgtattatc gcggtgatgt ccggcgggat tctgtttggc ccgtgggtag
    22681 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22741 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22801 tgaaaatccc caaagcacag cgctggcgct ggatatcgcc aacaaaacgc taccgctgtt
    22861 tcgccatgtc aatagcgagt cattacgcaa ggtctgcgaa attatccgcg atgacattca
    22921 cgccgatgcg gtggcgatta ctaataccga tcatgtgctg gcctatgttg gcgtgggtga
    22981 acataactat cagaatggcg atgacttcat tagcccgact acccgtcagg cgatgaatta
    23041 cggaaaaatc atcattaaaa acaatgatga agcccaccgc acaccagaga ttcattccat
    23101 gctggtgatc ccattgtggg agaaaggggt cgtgaccgga acgctgaaaa tttactactg
    23161 ccacgcgcat cagatcacct cgtcattaca ggaaatggcg gtcggtctgt cgcaaatcat
    23221 ctccacgcaa ctggaggttt cacgcgccga gcagctacgt gaaatggcaa ataaggcaga
    23281 gattcgcgcc ctgcaaagca aaattaatcc ccattttctg tttaacgctc tgaacgctat
    23341 ttcatcgtca atccgtctga atccggatac cgctcgccag ttgatcttta atctgtcgcg
    23401 ttatctgcgc tataacattg aattaaaaga cgatgagcaa atcgatatca aaaaagagct
    23461 gtatcaaatt aaagactata ttgccattga gcaggcccgc tttggcgaca agctgacggt
    23521 tatctatgat attgatgaag aggtgaattg ctgcattccc agcctgctga tccagccgtt
    23581 ggtggagaac gccattgtcc acggtattca gccttgcaaa ggtaaaggcg ttgtcaccat
    23641 cagcgttgca gagtgcggaa atcgggtacg cattgcggtg cgagataccg ggcacggcat
    23701 cgttccaaag gtgattgagc gggtcgaagc gaatgaaatg ccgggcaata aaattggcct
    23761 gctgaatgtc catcatcgcg tgaagttatt gtatggcgag gggctgcata tccgccgcct
    23821 ggagccgggg acggaaattg cgttttacat tcctaaccaa cgcaccccag tcgcctcaca
    23881 ggctacgtta ttgctttgag ccggagtgat attgtgaaag tcatcattgt tgaagacgaa
    23941 ttcctggcac aacaggaact gagctggcta attaaagagc acagccagat ggagattgtc
    24001 ggcacctttg acgacggtct ggacgtgttg aagtttttgc agcataaccg cgtcgacgcc
    24061 atttttctgg atatcaatat tccgtcgctg gatggcgtgt tgctggcgca aaacatcagc
    24121 cagttcgccc ataaaccgtt tattgtgttc atcaccgcgt ggaaagaaca tgcggtagaa
    24181 gcgtttgaac tggaggcgtt tgactacatt ctcaaaccgt atcaggagtc acgtattacc
    24241 gggatgctgc aaaaactgga agcggcctgg caacaacagc agaccagcag tacgcctgcc
    24301 gcgacggtaa cgcgtgagaa tgacaccatt aatctggtga aagatgagcg aataatcgtc
    24361 acgccaatta acgatatcta ttacgccgaa gcgcacgaga aaatgacctt tgtctatacg
    24421 cggcgtgaat cctacgtaat gccgatgaac attaccgaat tttgcagcaa actgccgccg
    24481 tcgcattttt tccgctgcca tcgctcattt tgtgtcaatc tgaacaaaat acgcgaaatc
    24541 gaaccgtggt ttaataacac ctacattctg cgactgaaag atctggattt tgaagtgccg
    24601 gtcagccgca gcaaagtgaa agaatttcgc cagttaatgc atctttaatc gggaactttc
    24661 atgaaagcac caggcttgcc cgccgatcaa caatttttcg ccgatctgtt cagcggccta
    24721 gtgcttaacc cgcaactact ggggcgcgtc tggtttgcca gccagcctgc ctcgttaccg
    24781 gtgggcagtt tatgtattga ttttccccgt ctggatatcg tgctgcgcgg cgaatacggc
    24841 aacctgctgg aagcaaagca gcagcgaatg gtggaaggag aaatgctgtt tattccggca
    24901 cgcgcggcta atttaccgat caacaacaaa ccggtgatgc tgttaagcct ggtgttcgca
    24961 ccgacctggc ttgggttatc gttttacgat agccgcacca cgtcgttgtt gcatcctgct
    25021 cgccagaccc agcttcccag cctgcaacgc ggtgaaggtg aagcgatgct taccgccctc
    25081 acccatctta gccgttcgcc gctggagcag aatatcattc agccactggt gttaagtttg
    25141 ctgcatcttt gccgcaacgt ggtgaatatg ccgccgggta attcacaacc gcgcggcgat
    25201 tttctctatc acagcatttg taactgggtt taggataatt atgcccagcc gctcacccgc
    25261 gagagcgtgg cgcagttttt taatatcacg cccaatcatc tgtcaaaact gtttgctcag
    25321 catggaacga tgcgttttat cgagtatgtg cgttgggtgc gaatggcgaa ggcgaggatg
    25381 attttgcaga aatatcatct gtcaattcat gaagtggcac agcgttgcgg ttttccggat
    25441 agcgactatt tttgtcgcgt tttccggcgt cagtttagtc tgacgccggg agagtacagc
    25501 gcccgttttc agggctaacg tcagaaggtt aattctgttt ccagcagcgt caggatactt
    25561 tgtgcatctt gcgcggcaaa caacgactgg cggaagtttt tattcaccag tttacgcgcc
    25621 agctgcgaga agactttcac atgattcatc ccttcgttag cacccagcgt cagcatgatc
    25681 accagttcga cttcgcccat ttctgactgc cagtcaaccg gtttcgccag ccgggcaatg
    25741 ctgatactgg aatgacggat ccactgagat ttggtgtgcg ggatcgctac gccaaaacca
    25801 acgccggtgg taacaatctc ttcccgctgc cagacatctt cttccagctc aaacggatgt
    25861 tcagtgcgcc cgttaacgcc gaggttgccg cacaggaact ggatcgcctg ctctttattg
    25921 ctaaaatcct gatcaacaaa gatattctcc agcgccagca gtgggcgaac gtcttcttcc
    25981 ggcgtaaagg cggtgagtaa cgcttcaatt tcctgcgcac tgcggcattc acatgcctga
    26041 cgcgccagtt cccgacacgc ctcgctatcc agttgacgaa gctggctttt caccgccgga
    26101 atacgcgggc tactcatact cagctcatcc aggcccagcc caagcagtag cggcagataa
    26161 cggctttcac cgcccagttc accgcaaatg cctacccatt tgccccgctg atgcgcagtg
    26221 gtaactattt gctgcaacat gcgcaggaac gatggcgtaa tcgggttata tagcggcgat
    26281 acgcgcgggt tattacgatc gaccgcatac agatactggg tcatatcgtt ggagccgata
    26341 ctgaagaaat cgacctcatc gcagaagtgg tcgatgatgt agcacaccga cggcacttcc
    26401 accatgatcc caagcgtaat cgtctctgca tgacgcaggc catcgcgctt aagctcaacg
    26461 atcgcttttt ggatctcgcc tttcacccat aagatctgat cgaggctgtg aaccatcggg
    26521 atcatcaact gggcgttgcc gaaactggcg gcgcgcaaaa tggcccgcag ttgagtgcgg
    26581 aacaggccag caaattccgg gtaaatacgt accgcgcgat agccgaggaa cgggttctct
    26641 tcctggggaa tattcagata aggaatgctt ttatcgccgc cgatgtccat cgtgcggaag
    26701 ataatcggct tgtcgcccgc cgccagtagc acctgctggt aggcttcaaa ttgctcctgc
    26761 tcgtccggcg cgctgtcgcg atccatatag agcatttcgg tacggaacag cccgacacct
    26821 tccgcgccgt tggcaaacgc gcctggcgct tccagagcgg tgccgatatt cgccgcaata
    26881 tcaatacgct tgttatcacg ggaataggcc agctgcgcgg cagcctgcgc ctgttgtttt
    26941 tggcgtttat ccgccagcgt ctgcgcgacc tgataataac cgctcaccgc gtcattcggg
    27001 ttaatcgcca gcacgccgca ctgggcgtca agcactgcag gttgcccggc ataacgggca
    27061 atcgcatcca gcggcaagcc actcagtacc gggatcgccg aggcacgggc cagaatcagt
    27121 gtatgcgagg tgcgcccggt tttctccaga atcatacccg caagattttt caaatcaagg
    27181 ctcaaaaact ggcttggggt taaatcttca gccaccagaa tggtcggttt ttccagcacc
    27241 agattgttgc gcggcttcag ttccggccag gtgatatgca gcaactgttc gctgatatcg
    27301 cgaatgtcgc tgacccgctc gcgcagataa tcgctggcag aagcagatag tttggcgcaa
    27361 acctgctcca tattgctgat gatcgccgcc cccagtccct gatgctgttc tgtcatcagg
    27421 cgacggatat tgcctgcaaa ttcatcatcc tgaatcagcg acaaatgagc gctgaggata
    27481 gttttgcttt cgccgtcacg ctcacgcaat tgctgattca gttgctcggc aagcgttgcc
    27541 aggctgtgct ccagccgggt ggaatcttgc gcactggcgg ggattgcccg ataactgtcg
    27601 aggctgtcgc tttgtaacag ggtcagcgta cccacgccga cgccgcttgc cagcacattg
    27661 ccgtacagta aatccgggtt aaggcggctt aatgaacgcg gcagcggatg cgccgtcagt
    27721 tctgcctgcg tcggctgaac gctgtcgctg tcgataaagc gcaccaggat gtactcttcc
    27781 agcacgcgcc gcgcctgctc ttcatcgctg ccgctaatgt tcaggctgca actgtcatta
    27841 aataaggtgc cagttccaat caacgccagc gagcttttag catcagcctt tgcgttctgg
    27901 cgatggttga taaaggtgat ttcgctttgc cactggctgc actgttcttt aagttcccac
    27961 gccggacgag catgtagacc gttaggcaaa ggacagagaa attgaatcgt taacataacc
    28021 gctccgggtt aatcaggaga tcatctgaaa tccgtcagtt gaacaaccgt ctcacgcgta
    28081 agacgttgaa taagggcaga taaaagttgc tccatctgca aaatgtcgcg gcaatcggca
    28141 atcgatgccg cgcaatgtcc gtggcgggtg gcaggcccca ttaccaatgt gggtacgcca
    28201 gtaccggtta agtgcaccgc tccaccgtcc gtgccgccgt tactgaacat atccgcctgc
    28261 aacggaacgc caatttctgc cgccacggtt tcaatccacg cggtaagttt tggcggcgca
    28321 atcagcgatt tatcgcttaa caccagcatc ggcccgttac caatctgacg atggttagcc
    28381 gcgccataat caaagttttt cgcccagcag gcggtatcaa gcacaatggc gacgtccggc
    28441 gacaccgcgc gggtagcagt ttgcccgccg cgtaatccca cctcttcgct ggaacttgcc
    28501 accagccaca cttccgcagg cagttcggcg tcgtgcagct cacgtagtaa cgtcaccagc
    28561 agatagcaac tgaggcgatc atcaaaggct ttgcccatca cccgctggtg agggaggacc
    28621 tgaaaagtgg tatcgaaggt gacgcgatcg cctggacgaa ttcccgcctg catcacttcg
    28681 tcataggtgc gcgcgccaat gtccacgcgc atggcgctaa cgtcattccc ctgccggtcg
    28741 ccgtcaagca ggcctggaat tttgcactct tcacgggtgg tgatgcgcac cggctgcagc
    28801 tggcgggcag ccatgcgcac gttgccaacc ggcagcacgt caatagcgcc ttcgcgggag
    28861 atgctgcgca ccataaatcc cacttcgtcc atatgggcgc agatcatcac cttcggacct
    28921 gtcgattcat tgaggcggat cagcaccgat cccagaccat caaatcgcac ttctttttgc
    28981 aggcgagccg cttcttccag caggatctgc cgcacttcct gttccgagga ggcgatcgca
    29041 tctgcctcgc tcaacgcttt taatagcgat aaatccatta tgcctctccc gtgagcaaca
    29101 ctgttttcgg catggcgtag agcacttctg cgccttgcgg ggtaaccagc acaacatctt
    29161 cgatgcgcac gcccccttgc cctagcaaat aaatccccgg ctccacggtc agcaacatgc
    29221 ctggctgtag cgtcgtggtg tcccgcggtg aaaaacgcgg atcttcatgg acttcaatgc
    29281 cgatagcgtg accagtgtta tgaccgaaat agtcgccaaa tccagcctcg gtaatgaccc
    29341 ggcgcgcggc gtcgtcaatc tgctggcagc gcacgccggg gcgaattgca gagattgccg
    29401 cgagctgtgc ctgcaggaca atctgataga cgtcaaacag cgggtgagat tcggcgctca
    29461 ccccttcgcc attcaccagc aaggtgcgcg tcatatcagc agtagccctg atacagcgca
    29521 ccaaaatcga gagtgacaaa ctcgcccgct gcaacaatct tgtcgctggc tttgccgtgc
    29581 ggcagcgccc cgcgccagcc gctggcgaca atggtgtcaa aagaggcttt ttctgcgccc
    29641 tgctggcgca taaaccactc cagttcagcg gctatctcgc gctcgctcat ccccgcctga
    29701 ataaagcggc gaatatgctc cgctccgcga tcggcaatcc cacaggcgag gcggattatc
    29761 tccacctcct ctggcgtttt gatttgccgc agcacatccg gcgtggcgct aaccagtttc
    29821 gcattgagtt cagacttcca gcggtgcgcg gtttcccaac tcacctgctg gccttcaaaa
    29881 ccgagcgttt gcaactgctc atcggcaatg atttgattga cgatagtggt aagcgtgttc
    29941 gttgcgtcaa gcaaatgcag ctggtagcct tgcgcacggg cttccacctc aacgaaatag
    30001 cgcgaatcta ccagaatgtg cgcactttca cggctaataa ccacatagcc tgatccggtg
    30061 gagatcccca gatgcggctg tttgttctgc cgtgaggaga gaagcaccgc atccagttgt
    30121 tgcaccttaa gccagttgcg cagcgaagcg agtaatgtca tctggatttt ccttcttaca
    30181 ggctatcgat taacaatttg cctttgcgga acatcatcag acgcaggaac accaccatca
    30241 gcgcagtaat gattgccccc agcgcgatcc ccgccatata aacgcccaag ttagtgacca
    30301 gcggccatgc ccagatagcg gattccggga accattgcac tgcgcccagc cagacagctg
    30361 cggttgagcc gacaatcgcg ccgaccatat atgacggaat agcggtgatg gggctttcca
    30421 gcgcaaacgg aatcgcaccc tcactaatcc ccataaaggc caggaacatc gcggtcttac
    30481 cctgcggata aagttgagcg ttgaacaggc gtttgccggt taaacgacgg tcaataatgg
    30541 tcgccagacc cagaccaatc ggcggaataa cgatagcgat agaacgagcg gtgaccggta
    30601 atacgtggtc agtggtaaag ctgaaggcaa cgaaacctgc ggctttgttg atcgggccac
    30661 caaggtcgat tgctgtcgcc gcggcgatcc ccattgcgta catcagcgcg cccttctcac
    30721 ctgcggcagt cagcacagta cggataccgc cgttgatcca gccaccaaag ggagtgatga
    30781 cgtagtacat cgccagcatg acaaaaatgg cggaaagaat cggtagcaaa aatgtggttt
    30841 tgaacgctaa caggaaatcc ggtaactgga ttttctggtt catccatttc acaaggtaac
    30901 cggcaacgat agaaataatc agcgcaccaa tgaaggtgga cggcaccggc gaagaggtcg
    30961 cccattgcat agtgctggga tcgaagttca gcagttgcgt cggctgggta gacatcaacc
    31021 cgccgataaa accagccggg aacgccagtt tgccaccgat agagttcgcc acaaatgcag
    31081 caaacatcgg aatggcaaaa ccaaacaaca cgccgccgaa ggactgcgac agccaggcaa
    31141 atttaagtaa ggagaggtcg aaaccggaga atttcccgct attaagagca tccatgatgc
    31201 cgatctcagc aggaatttta agccagctat aagcaatcag ctggctgaaa gcgaggatca
    31261 caccgcccat aattaaggtc ggcaccatgc gtgaaatccc tgacatcaca tgctgcggga
    31321 gttcacccca gaagctgctt ttagaggcct gcggattctt aactgccgct gccgcaccgg
    31381 atgcgccagg cacaacggtt gcactgcgtt ttttaatggc cataacaatt atccctgtcg
    31441 aatattactg ttgttcagag gcaatcatct cttcgatttc tttgatgatg cccgcagcgt
    31501 ttttaattgc gtcctgcaaa gtgatttcat aaacgtcgcg tgattcgaaa cgttcgttat
    31561 cttccggggt aactgccacg gaatggatga tgatggtcgc ttcggcgata tcctgcgccg
    31621 tcaggcgatt ctggatgccg tccgcgccct gggtttcaat tttcacttca taaccggctt
    31681 ctaccgccgc ttcttccagc acctgagcgg ccataaaagt gtgagccagg cccattgggc
    31741 aggcacataa ggcaatcagt ttcttactca tcgttaattc ctcgttcagt gatagttgac
    31801 tagtgaaatt atgaacgtgc aaaacaaatc gccgttacca gacaaataat gcatttactg
    31861 gagaattaat cccgctgtac ggaagtgtga cgcaggtcgg ccttgtgagg ggacacaaag
    31921 aaaaattgaa ttaactgtat gatttaaaag attatcggga gagttacctc ccgatataac
    31981 aggaaggatt tacagaatgt gacctaaggt ctggcgtaaa tgtgcaccgg aaccgagaag
    32041 acccggatag tcatggacga tgagatacac cggaatatca tggacatatt ctttaaagcg
    32101 ccctttatct tcaaatgcgg cacggaaacc ggaggctttg aagaactcaa ggaagcgcgg
    32161 cacgataccg cccgcaataa acacgccgcc aaatgtcccg agattgagcg ccagattgcc
    32221 gccaaaacgg cccataatga cgcaaaacag cgacaatgcg cggcggcaat cggtgcagct
    32281 gtcagccagc gcgcgttcgg taatatcttt tggcttgaga ttttctggca ggcggttgtc
    32341 agctttcaca attgcgcgat acaaattcac cagcccaggg ccagaaagca cgcgctccgc
    32401 cgaaacatga ccaatttccg cacgcaatat ttcgaggata atggcctctt cttcactatt
    32461 cggcgcaaaa tcaacgtgac cgccttcgcc cggcaagctt acccaacgct tatcgacatg
    32521 gaccagatgc gcaaccccaa gccccgttcc ggcaccgtaa accgcaatag gcttaccttc
    32581 gaccggttct gcgccaccaa actgaatcag atgctctttt ttcagcatcg ggatcgccat
    32641 cgatacagcg gtaaaatcgt taataatttc cagatggcta aaaccgagat tctttttcat
    32701 ttcggcaatt gagaacgccc aggtatggtt ggtcattgcc acccagtcac cggtaattgg
    32761 gcaagcgatg gcaatacagc cgtctttcac ctcgacctta tgttcttcaa gataaacgcg
    32821 aatgaccgct tcgaggctgg ggtaatcaag ccctgaatag gtcttagcct gcgagatttc
    32881 accactggca atatcacaca gagcaagacg tgcgttggtg ccgcccacat caccgactaa
    32941 tgcatacttt gtcattcttc aactgctccg ctaaagtcaa aataattctt tctcacactg
    33001 taaatacctg ggggcataac aacaacgccg gaaaggcagg ctccctgtaa atatcgatct
    33061 gggtcacaca attactttat cgtttcagca ccaattgcag cgatgccttt ttgcaagctg
    33121 ggcaaactaa gtatctgacc ccgcataagg aatagaacat gctccatccg cgagccagaa
    33181 ccatgttgtt attatcgctc cccgccgtgg caattgggat tgcgtccagt cttattctga
    33241 ttgtggtgat gaaaatcgcc tcggtattac agaatttgct ctggcaacga ctgccgggaa
    33301 ctctggggat agcccaggat tcacccctct ggatcatcgg tgtattaacg ctaacgggta
    33361 ttgcggtggg gttggttatc cgtttcagcc agggtcatgc cggaccagac cccgcctgtg
    33421 aaccgctgat cggcgcaccg gttccgccct ctgcgctacc tagacttatc gtagcattaa
    33481 ttctcggtct tgctggcggc gtcagcctgg ggccggaaca tccgatcatg accgtcaata
    33541 tcgcccttgc cgtcgccatt ggcgctcgtc tgttaccgcg cgtcaaccga atggagtgga
    33601 ctattttagc ctctgccgga accatcggcg cgctgtttgg tacacctgtt gcggcggcgt
    33661 tgatattttc gcaaacctta aatggcagta gtgaagttcc gctatgggat cgtctttttg
    33721 cgccgttaat ggcggcagca gctggtgcac ttactaccgg attatttttc catccccatt
    33781 tttcactgcc cattgcgcat tacggacaga tggaaatgac cgatattctc agcggtgcaa
    33841 ttgtcgcggc gattgccatc gcagcaggga tggtcgccgt atggtgctta ccacggttgc
    33901 acgcgatgat gaatcaaatg aaaaatccgg tgctcgtgct gggtattggc ggatttattc
    33961 tcggaattct gggggttatt ggtggaccag tttcgctgtt taaagggctg gatgagatgc
    34021 agcagatggt ggcaaatcag gctttcagca ccagcgatta ctttttgctg gcggtaatta
    34081 aacttgccgc cctggtcgtt gctgccgcca gtggctttcg cggtgggcga atcttcccgg
    34141 cagtgtttgt cggcgtggca ttagggttga tgctgcatga gcacgttccc gccgttccag
    34201 cggcaataac cgtttcctgc gctattctcg gcatcgtgct ggtggtaaca cgcgatggct
    34261 ggttaagtct ttttatggcg gcagtcgttg tacccaatac cacattgcta ccgctgctct
    34321 gtatcgtcat gcttccggca tggctgttat tagcaggtaa gccgatgatg atggtcaatc
    34381 gtccgaagca acagccaccc cacgataatg tttagcaaaa aatgctctcc tttatgattt
    34441 aagagttatg gcgatgatac ggggctttac ctccccgtaa tattgcgtta acaggccgct
    34501 gacggcttcg atcgtgaagg agaataacaa tgttcaggtc actgtttctg gcggccgccc
    34561 tgatggcatt taccccgctt gcagcaaatg caggtgaaat caccctactg ccatcgatca
    34621 aattacaaat tggcgatcgc gatcattacg gtaattactg ggacggtggt cactggcgag
    34681 atcgtgatta ctggcatcgc aattatgaat ggcgcaaaaa ccgctggtgg cgtcatgata
    34741 atggctatca tcgtggctgg gataagcgta aagcatatga gcgtggctat cgtgaaggct
    34801 ggcgcgatcg tgacgatcat cgcggaaaag gccgcggaca tgggcaccgc cattaaacgc
    34861 gtcctccaat ggagcacaat gcctgatgcg acgctagcgc gtcttatcag gcctacaaac
    34921 cggctcttta acgcgtaggg cggataaggc gtttacgccg catccgccaa tagtgccaga
    34981 tgcgacgctc attcaactac aatcccagcg ccgtccccac caacaaccag atattcagcg
    35041 ccacgaccag cactacaatc acccagcctg tctgttttac gcgtttgctg ttcaccagat
    35101 cgcccatcaa cttgctgtca ctggtgaaaa tcagcagtgg aaccagcgcc agggcgatac
    35161 caaaacttaa cagcacctga ctcataacca gaatccgtgt cggatctaat cccatcagaa
    35221 tgacaataaa tgacggcaac atggtgactg tacgacgcac ccacagcggg atatggaagc
    35281 gaatgaatcc ctgcatcacc acctgccccg ccagtgtccc caccaccgtt gaggacagtc
    35341 cggcagcaac cagacttaac ccaaagaccg ttgccgcagc atggcttaac agcggttgca
    35401 gcgtcagata agcctcatca agatcggcaa caccagtatg accagaaaag tggaacgccg
    35461 ccgcagctgt agccatcatc accagattga caaaaccggc aatcgtcatg gcgatagcca
    35521 catcccattt ggtggcggaa taacgttgtt gacgcgaacc gccatgtaaa tgctgagtga
    35581 gcgaagagtg caaataaatc acatgcggca taatcgtcgc ccctaacacg cctgctgcca
    35641 ggaagaccgc ctccgaagta ggtaaactcg ggatcaccat tcctttaccc agctgcgcca
    35701 ggttaggctg ggagaaaatc aactcgacaa tgtaagccgc ggcaacaaac aacagtaacc
    35761 cgccaatcac tttctccagc ggtttttgcc cgcgacgttg cagcattaaa atcaggaaag
    35821 tcgcgatccc cgtcagcacc gcgccctgca acaacgaaac accaagaatg agtttaaaac
    35881 cgatcgccgc accaataaat tccgccaggt cggttgccat cgcaataatt tctgcctgaa
    35941 cccaatagaa ccacactacg ggacgcggat agtgatcacg aatctgctcc gccagatttt
    36001 taccggtggc aatccctagt ttggcagaga ggatctgaat cagcatcgcc atcaggttgg
    36061 cccaaacgac aacccacagt agctgatagc cgaagctagc acccgcctga atattggtcg
    36121 caaagttacc gggatcgata taaccaatcg ccgcaatgaa cgcaggtccc attaatgcga
    36181 gcctcatctt acgcgccgcc cgtccgctgc tactctcaac gcgatagttc gtcatcttgt
    36241 gcctctaaaa catagccttt gctatgtttc atgctatgct aaacgagaat gattatcaaa
    36301 ttcatttaaa tggattgtgg tgatttctct gatagaccaa gattatgatt acgaaaagat
    36361 tgatgacttg aatgttatgg ttatgtttaa tgttagcaca tttacataac tttcagcttc
    36421 catacacaac atagcagaaa tgtatgacag atcactattt ttgaagcctg tcacaggacg
    36481 tcattatagt gtgtgtcaga tctcgttttc cttaaccatg ttacatagaa tgtgcacgga
    36541 aatttaacct gcctcatatt tggagcaaat atggaccgcg tccttcattt tgtactggca
    36601 cttgccgttg ttgtgattct cgcactgctg gtaagcagcg accgcaaaaa aattcgtatc
    36661 cgttatgtta ttcaactgct tgttatcgaa gtgttactgg cgtggttctt cctgaactcc
    36721 gacgttggtt taggcttcgt gaaaggcttc tccgaaatgt tcgaaaaact gctcggattt
    36781 gccaacgaag ggactaactt cgtctttggt agcatgaatg atcaaggcct ggcattcttc
    36841 ttcctgaaag tgctgtgccc aatcgtcttt atctctgcac tgatcggtat tctccagcac
    36901 attcgcgtgt tgccggtgat tatccgcgca attggtttcc tgctctccaa agtcaacggt
    36961 atgggcaaac tggaatcctt taacgccgtc agctccctga ttctgggtca gtcagaaaac
    37021 tttattgcct ataaagatat cctcggcaaa atctcccgta atcgtatgta caccatggct
    37081 gccacggcaa tgtccaccgt gtcgatgtcc atcgttggtg catacatgac catgctggaa
    37141 ccgaaatacg tcgttgctgc gctggtactg aacatgttca gcacctttat cgtgctgtcg
    37201 ctgatcaatc cttaccgtgt tgatgccagt gaagaaaaca tccagatgtc caacctgcac
    37261 gaaggtcaga gcttcttcga aatgctgggt gaatacattc tggcaggttt caaagttgcc
    37321 attatcgttg ccgcgatgct gatcggcttt atcgccctga tcgccgcgct gaacgcactg
    37381 tttgccaccg ttactggctg gtttggctac agcatctcct tccagggcat cctgggctac
    37441 atcttctatc cgattgcatg ggtgatgggt gttccttcca gtgaagcact gcaagtgggc
    37501 agtatcatgg cgaccaaact ggtttccaac gagttcgttg cgatgatgga tctgcagaaa
    37561 attgcttcca cgctctctcc gcgtgctgaa ggcatcatct ctgtgttcct agtttccttc
    37621 gctaacttct cttcaatcgg gattatcgca ggtgcagtta aaggcctgaa tgaagagcaa
    37681 ggtaacgtgg tttctcgctt cggtctgaaa ctggtttacg gctctaccct ggtgagtgtg
    37741 ctgtctgcgt caatcgcagc actggtgctg taaaaccata cataaaaaag ccggggatca
    37801 ttccccggct tttttacgcc tgttaatcag ctaatggctg cgggcgacca atcaaatacc
    37861 cttgcagata ttgcaccccg agcttatgca atagcgcctg ctgctgttgc gtctcgacaa
    37921 actccgcgac cacactcaat gacttcgctt tcgccagatc ggtaattgat ctcacaatca
    37981 tcgcatccag cgtattcgtg acaatatctt tcacaaagac gccatcaatt ttgatgatat
    38041 cagcctgcaa acgctttaac cgttcgtagt tggcatatcc ggtgccaaag tcatcaatcg
    38101 caatccggaa accaaactta tgcagctgct cgatgttgta catgctgctt tctgcgttag
    38161 aaaacgcctg ctcctcggtg atctcaagaa tgaccgcctg cggggagatg tgataacgct
    38221 taaacagacg aataatccgc ccggcaatat tcttttgcag cagcgtgagc ggcattaaat
    38281 tgactgaaaa gcgcggtcct tttttgtcgc aagggtgtgt tgccagccac ttcaacaagg
    38341 attccagcac ttgcaaatca aaacgcgcgc taaggttaaa ctgagcaata aggggcagaa
    38401 acttatccgg ggtcataatg ccgccgtcat atttcagtcg cgcgaggatc tcatcataac
    38461 cttcaccctc tttgttgcga attggctggg cgtagagcag caaatcaccc tgatctaacg
    38521 ccgtgcgaat ggtattcagc aatagcacct gtttagtggt ctgcccggaa accatctcct
    38581 ctctgctatc cagcgccagc acatgatgat gtgcgcagga ttgctccgcc agccagctta
    38641 actgccccaa caagggttgc agggtttcct gatttccatc aaaacgcccc caggcagcac
    38701 catagcccat atccagcccg gtattgttcc agtgaatttg ccgactattc aggatgttaa
    38761 ccatatgctg gagtcgccct tccgtttccg gcccgctcag cactaacagc agttcactac
    38821 ccggcaattg atacaacttt tcgttttcct gcatcaacgg cagcagcgta cggcaaattg
    38881 agcggataca gtgaacgcgc atcattaagc cgtaatgacg actcataaac tcaagattat
    38941 caatgcgcag gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgt tccaacgcac
    39001 gaaagttggg taggagcgtt aacggatctg ttagcgcctg caaatgccag cggcgattaa
    39061 gccattcact gcgatgataa atgcgcacca tatagagcag gcaaacgcta aaggaaatca
    39121 gcaccgcgag aataaatgcc agcgaatatt cggtttccac cccttgcaaa aagttctgat
    39181 tgtaattcag aaggcaaagc gttgaaacag cccaggtgag atttaaaaac ggatagcgaa
    39241 gattaccgac cccgagggta aagatgatga agaaaacggg taccaggtaa ccggcaataa
    39301 agtcgttttc ataaggtgtg cacaacagaa gtagcagcac gcttaacgct gccagccagc
    39361 ttaaggtaaa tgcgagtgag atagtagaaa agcatgttgt aaatcagcac tgcggtgaaa
    39421 aggcttagta aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc
    39481 tttagcggga aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc
    39541 gtcaccaaac caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc
    39601 caacgcgtcc ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg
    39661 ctgccgagca tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa
    39721 aacatcccgc ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc
    39781 attacgcaca gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc
    39841 gaaataaaac gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc
    39901 ttgatatttt ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg
    39961 gttttcaggc acacaaataa taagtttatt cgcatcgcga agcaaacagg ggccagcctg
    40021 ttgccgattt ttcatccaat gatgctttca ccaggcggcg ctaacccgga cgtagactgt
    40081 attacaaaag cggcaaaaag cagagacaaa aaacccccgc tttgcagcga gggttggaaa
    40141 tttggtggag ctaagcggga tcgaaccgct gacctcttgc atgccatgca agcgctctcc
    40201 cagctgagct atagccccac gatgcgttta cgtaccaaat ttgctgggtg caaaatttgg
    40261 tggagctaag cgggatcgaa ccgctgacct cttgcatgcc atgcaagcgc tctcccagct
    40321 gagctatagc cccgtcacgt aaagcttgtc gagttgacgg gcggcatcat atgaattccg
    40381 cccgaatgtg tcaacggcaa attgcaacgt gtagtttcaa tcgctgaaaa atcaggcaaa
    40441 tgaacaattt tggaaaaccg ctcgcattca gtagttattc atgtcacggt ttcctgtaaa
    40501 gtggtgttat aaaatgaact actaatagac ccacatacat tcagggaatt gttatgttca
    40561 aggagaggat gacaccagat gaacttgcca gactgaccgg ctatagccgc cagaccatta
    40621 ataaatgggt acgcaaggaa ggctggacaa cgtcaccaaa acctggcgtc cagggtggca
    40681 aagcccgact ggttcacgtc aatgaacagg ttcgtgaata tattcgcaat gctgaacgtc
    40741 cagaaggtca gggagaagcg cctgcccttt ctggtgatgc gccgcttgaa gtattgctgg
    40801 tgacactggc gaaagagatg acgccagttg aacaaaaaca gtttacatcc ttgcttctgc
    40861 gggaagggat tatcggattg ttacaacgct tagggattcg cgatagcaaa taatatgaaa
    40921 agattacgca ataaaatgac caccgaagaa ctggctgaat gcctcggtgt ggctaaacaa
    40981 actgttaacc gttggaccag agaaaaaggc tggaaaacgg aaaaatttcc cggcgtgaaa
    41041 ggcggtcgtg ccagacttat tctggtcgat acgcaagttt gcgagtttat tcagaacacg
    41101 ccagccttcc ataacacacc aatgttgatg gaagctgaag agagaattgc tgaatacgct
    41161 cctggagcac gcgccccggc gtatcgacaa atcatcaacg cgatagataa tatgacagac
    41221 attgagcagg aaaaagtcgc gcaatttttg tcacgtgaag gaattcgcaa tttcctcgcc
    41281 cgtctcgata ttgacgaatc agcataaata aaaaacggca ggatattatc tcctgccgtt
    41341 tatcttttta cacgctaatt actgctgatt ttcgcgttca gcaataaaat ccagcgcttt
    41401 gttgatacgc tcgatactgc gggtcttacc aattgcgtga acggtaacat ccagtgctgg
    41461 agactgcccc gcaccggtta cggcgacacg cagcggcata ccaactttac ccatacccac
    41521 ttccagctca tccgccgtcg cctgaatagc gtgatgaacg ttttcagcgg tccagtcagt
    41581 aatcgcggcc agtttgtcac gaaccacttc cagcggctga cgcgctaccg gacgcagatg
    41641 ttttttcgcc gcatcggcat cgaactcagc aaaatcttcg tagaaataac ggcagctctg
    41701 tgccatctct ttcagcgtct tgcagcgttc gcccagcagt ttcaccagat cagccagctg
    41761 cgggccgtta cgggtatcga tattttcctg ctcaatgtgc cactgtaagt gagtagcaac
    41821 atactccggc ggcagcgcgt taatgtagtg atggttcagc cacagcagct tgtcggtgtt
    41881 gaacgcactg gcagatttgc tgacggcatt caaagtgaag tatttgatca tctcttcacg
    41941 agtgaagatt tcctgatcgc cgtgggacca gcccagacgc accagatagt tcagcagtgc
    42001 ttctggcaaa taaccgtcat cacgatactg cattacgctg actgccccgt gacgtttgga
    42061 cagtttttta ccgtcatcgc cattgatcat agaaacgtgc gcgtaaaccg gcaccggcgc
    42121 tttcagggcc ttaagaatgt tgatctggcg cggcgtgttg ttgatatggt cttcgccacg
    42181 gataacgtgg gtgatttcca tatcccagtc atcgacaacc acacagaagt tataggttgg
    42241 ggaaccatcg gtacggcgga taataagatc gtccagttcc tggttgctga actcgatcgg
    42301 accacggatc tgatcgtcaa aaacaacaga accttcctgc gggttagcaa aacgtacaac
    42361 acacggttca tcatcagcat gatgctcatg gctgtggcgg cagcgaccgt cataacgcgg
    42421 cttctcacct ttcgccattt gctcttcgcg cagcgcttcc aggcgctctt tagagcaata
    42481 gcatttataa gcagtgccct cttccagcat ctgatcgatc accgcgttgt agcgatcaaa
    42541 acgtttggtc tggtagtacg gaccttcatc ccactccagg ctcagccagt tcatgccatc
    42601 cataatggct tcgatagctt ccggcgtgga acgctcaaga tcggtgtctt caatacgcag
    42661 cacgaactca ccgccgtggt tacgtgcaaa aagccaggag taaagagcag tacgcgcgcc
    42721 gccaacgtgc agatagcctg ttgggcttgg cgcgaagcga gttttgattt tcatgaaatg
    42781 gccttacgtt tagaaagatg ccgacaaccg gcaaatcctg gaaaaattga gtgggcgata
    42841 ttctatcact cccgcctgat tcctcaatgt agttcgggtt tctaccttac gcattattgt
    42901 tttcgttgac aaattgcgca acacggtctg ttttgcgatc atcttgctta aatttacgac
    42961 gaacgaacaa tttctttaga aaatgcgttg actcattttg aactctccct ataatgcgac
    43021 tccacacagc gggggtgatt agctcagctg ggagagcacc tcccttacaa ggagggggtc
    43081 ggcggttcga tcccgtcatc acccaccaac tactttatgt agtctccgcc gtgta
//
LOCUS       DAJTUP010000024        42374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTUP010000024 DAJTUP010000000
VERSION     DAJTUP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.024"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..417)
                     /gene="ssb1"
                     /locus_tag="OO468_001340"
     CDS             complement(<1..417)
                     /gene="ssb1"
                     /locus_tag="OO468_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR6258514.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQP"
     gene            671..3493
                     /gene="uvrA"
                     /locus_tag="OO468_001341"
     CDS             671..3493
                     /gene="uvrA"
                     /locus_tag="OO468_001341"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR6258515.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3528..3884)
                     /locus_tag="OO468_001342"
     CDS             complement(3528..3884)
                     /locus_tag="OO468_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6258516.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(3888..4304)
                     /locus_tag="OO468_001343"
     CDS             complement(3888..4304)
                     /locus_tag="OO468_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR6258517.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4415..5128)
                     /gene="aphA"
                     /locus_tag="OO468_001344"
     CDS             complement(4415..5128)
                     /gene="aphA"
                     /locus_tag="OO468_001344"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR6258518.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5530..5734
                     /locus_tag="OO468_001345"
                     /pseudo
     CDS             5530..5734
                     /locus_tag="OO468_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6237..7430)
                     /gene="tyrB"
                     /locus_tag="OO468_001346"
     CDS             complement(6237..7430)
                     /gene="tyrB"
                     /locus_tag="OO468_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR6258519.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7683..8762)
                     /gene="alr"
                     /locus_tag="OO468_001347"
     CDS             complement(7683..8762)
                     /gene="alr"
                     /locus_tag="OO468_001347"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR6258520.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8815..10230)
                     /gene="dnaB"
                     /locus_tag="OO468_001348"
     CDS             complement(8815..10230)
                     /gene="dnaB"
                     /locus_tag="OO468_001348"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6258521.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10313..11296
                     /locus_tag="OO468_001349"
     CDS             10313..11296
                     /locus_tag="OO468_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR6258522.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11462..11704)
                     /gene="pspG"
                     /locus_tag="OO468_001350"
     CDS             complement(11462..11704)
                     /gene="pspG"
                     /locus_tag="OO468_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR6258523.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11838..12875)
                     /gene="dusA"
                     /locus_tag="OO468_001351"
     CDS             complement(11838..12875)
                     /gene="dusA"
                     /locus_tag="OO468_001351"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR6258524.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13238..14223)
                     /locus_tag="OO468_001352"
                     /pseudo
     CDS             complement(13238..14223)
                     /locus_tag="OO468_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14541..15056
                     /gene="zur"
                     /locus_tag="OO468_001353"
     CDS             14541..15056
                     /gene="zur"
                     /locus_tag="OO468_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR6258525.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15098..15307)
                     /locus_tag="OO468_001354"
     CDS             complement(15098..15307)
                     /locus_tag="OO468_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR6258526.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15423..16748)
                     /gene="dinF"
                     /locus_tag="OO468_001355"
     CDS             complement(15423..16748)
                     /gene="dinF"
                     /locus_tag="OO468_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR6258527.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16821..17429)
                     /gene="lexA"
                     /locus_tag="OO468_001356"
     CDS             complement(16821..17429)
                     /gene="lexA"
                     /locus_tag="OO468_001356"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR6258528.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17539..17907)
                     /gene="dgkA"
                     /locus_tag="OO468_001357"
     CDS             complement(17539..17907)
                     /gene="dgkA"
                     /locus_tag="OO468_001357"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR6258529.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18078..20501
                     /gene="plsB"
                     /locus_tag="OO468_001358"
     CDS             18078..20501
                     /gene="plsB"
                     /locus_tag="OO468_001358"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR6258530.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20656..21528)
                     /gene="ubiA"
                     /locus_tag="OO468_001359"
     CDS             complement(20656..21528)
                     /gene="ubiA"
                     /locus_tag="OO468_001359"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR6258531.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21541..22038)
                     /gene="ubiC"
                     /locus_tag="OO468_001360"
     CDS             complement(21541..22038)
                     /gene="ubiC"
                     /locus_tag="OO468_001360"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR6258532.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22261..23841)
                     /locus_tag="OO468_001361"
                     /pseudo
     CDS             complement(22261..23841)
                     /locus_tag="OO468_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24070..24990)
                     /gene="malM"
                     /locus_tag="OO468_001362"
     CDS             complement(24070..24990)
                     /gene="malM"
                     /locus_tag="OO468_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR6258533.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25142..26482)
                     /gene="lamB"
                     /locus_tag="OO468_001363"
     CDS             complement(25142..26482)
                     /gene="lamB"
                     /locus_tag="OO468_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR6258534.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26554..27669)
                     /gene="malK"
                     /locus_tag="OO468_001364"
     CDS             complement(26554..27669)
                     /gene="malK"
                     /locus_tag="OO468_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR6258535.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28034..29224
                     /gene="malE"
                     /locus_tag="OO468_001365"
     CDS             28034..29224
                     /gene="malE"
                     /locus_tag="OO468_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR6258536.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29378..30922
                     /gene="malF"
                     /locus_tag="OO468_001366"
     CDS             29378..30922
                     /gene="malF"
                     /locus_tag="OO468_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR6258537.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            30937..31827
                     /gene="malG"
                     /locus_tag="OO468_001367"
     CDS             30937..31827
                     /gene="malG"
                     /locus_tag="OO468_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR6258538.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPISI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32198..33673
                     /gene="xylE"
                     /locus_tag="OO468_001368"
     CDS             32198..33673
                     /gene="xylE"
                     /locus_tag="OO468_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR6258539.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33717..34127)
                     /gene="psiE"
                     /locus_tag="OO468_001369"
     CDS             complement(33717..34127)
                     /gene="psiE"
                     /locus_tag="OO468_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR6258540.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34253..34396)
                     /locus_tag="OO468_001370"
     CDS             complement(34253..34396)
                     /locus_tag="OO468_001370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258541.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34397..34675
                     /locus_tag="OO468_001371"
     CDS             34397..34675
                     /locus_tag="OO468_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258542.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34722..36818)
                     /locus_tag="OO468_001372"
     CDS             complement(34722..36818)
                     /locus_tag="OO468_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6258543.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNNLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(36818..37555)
                     /locus_tag="OO468_001373"
     CDS             complement(36818..37555)
                     /locus_tag="OO468_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6258544.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37552..38190)
                     /locus_tag="OO468_001374"
     CDS             complement(37552..38190)
                     /locus_tag="OO468_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6258545.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38304..38546)
                     /locus_tag="OO468_001375"
     CDS             complement(38304..38546)
                     /locus_tag="OO468_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258546.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39009..40658)
                     /gene="pgi"
                     /locus_tag="OO468_001376"
     CDS             complement(39009..40658)
                     /gene="pgi"
                     /locus_tag="OO468_001376"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR6258547.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41183..>42374
                     /gene="lysC"
                     /locus_tag="OO468_001377"
     CDS             41183..>42374
                     /gene="lysC"
                     /locus_tag="OO468_001377"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR6258548.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLS"
BASE COUNT        10547 a        10914 c        10393 g        10520 t
ORIGIN      
        1 aggctgaccc caaccgccct gcggctgacc accaccgata ttgccacctg ccggagcgcc
       61 accaccctga cgaccaccca gcatctgcat ggtgccgcca acgttcacca cgacttctgt
      121 ggtgtagcga tcctgaccgg attgatcggt ccatttacgg gtacgcagct gaccttcgat
      181 ataaacctga gaacctttac gcagatattc gctcgccact tctgccagtt tgccgaacag
      241 cacaacgcgg tgccattcag tctgttcttt catctcgccg gtcgctttat cacgccagga
      301 ttcggaagta gccagcgtaa tgttggcaac tgcgccacca tttggcatgt agcgtacttc
      361 cgggtcctga cccagattac caacgagaat aaccttgttt acgcctctgc tggccatgtt
      421 cgtgtctcct gaaaaaaatc gttctgaata agtgtaaacg cgcgattgta ccattaccaa
      481 tagcgctttt actatgttgt gacctcggtt ccgggaaaca aacctggcca gacattgtta
      541 cacaacactc cggataatgc attccaatac tgtatattca ttcaggtcaa tttgtgtcat
      601 aattaaccgt ttgtgatcgc cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg
      661 ggaaaggtga atggataaga tcgaagttcg gggcgcccgc acccataatc tcaaaaacat
      721 caacctcgtt atcccccgcg acaagctcat tgtcgtgacc gggctttcgg gttctggcaa
      781 atcctcgctc gctttcgaca ccttatatgc cgaagggcag cgccgttacg ttgaatccct
      841 ttccgcctac gcgcggcagt ttctgtcact gatggaaaag ccggacgtcg atcatattga
      901 ggggctttct cctgccatct caattgagca gaaatcgacg tctcataacc cgcgttctac
      961 ggtggggaca atcaccgaaa tccacgacta tttgcgtttg ttgttcgccc gcgtcggcga
     1021 accgcgctgt ccggaccacg acgtcccgct ggcggcgcaa accgtcagcc agatggtgga
     1081 taacgtgctg tcgcagccgg aaggcaagcg tctgatgctg ctcgcgccaa tcattaaaga
     1141 gcgcaaaggc gaacacacca aaacgctgga gaacctggca agccagggtt acatccgtgc
     1201 tcgtattgat ggcgaagtct gcgatctttc cgatccgccg aaactggaac tgcaaaagaa
     1261 acataccatt gaagtggtgg ttgatcgctt caaggtgcgt gacgatctta cccaacgtct
     1321 tgcggagtcg tttgaaaccg cgctggaact ttccggtggt acagcggtag tggcggatat
     1381 ggacgacccg aaagcggaag agctgctgtt ctccgccaac ttcgcctgcc caatttgcgg
     1441 ctacagtatg cgtgaactgg agccgcgact gttttcgttt aacaacccgg caggtgcctg
     1501 cccgacctgt gacggccttg gcgtacagca atatttcgat cctgaccgcg tgatccaaaa
     1561 ccccgagctg tcactggctg gcggtgcgat ccgtggctgg gatcgccgca acttctatta
     1621 cttccagatg ctgaaatcgc tggcagatca ctataagttc gacgtcgaag cgccgtgggg
     1681 cagcctgagc gcgaacgtgc ataaagtggt gttgtacggt tctggcaaag aaaacattga
     1741 attcaaatac atgaacgatc gtggcgatac ctccatccgt cgtcatccgt tcgaaggcgt
     1801 gctgcacaat atggagcgcc gttataaaga gacagaatcc agtgcggtac gtgaagaatt
     1861 agccaagttt atcagcaatc gcccatgcgc cagctgcgaa gggacgcgtc tgcgtcggga
     1921 agcgcgccac gtttatgtcg agaatacgcc gctgcctgct atctccgaca tgagcatcgg
     1981 tcatgcgatg gaattcttca acaatctcaa actcgctggt cagcgggcga agattgcgga
     2041 aaaaattctt aaagagatcg gcgatcgcct gaaattcctc gtaaacgtcg gcctgaatta
     2101 cctgacactt tcccgctcgg cagaaacact ttccggcggt gaagcccagc gtatccgtct
     2161 ggcgagccag attggtgcgg gcctggttgg cgttatgtac gtgctggacg agccgtctat
     2221 cggcctgcac cagcgcgata acgagcgcct gttgggtacg cttatccatc tgcgcgatct
     2281 cggtaatacc gtgattgtgg tggagcatga cgaagacgca attcgcgccg ctgaccatgt
     2341 gatcgatatc ggtcctggtg cgggtgttca cggcggtgaa gtggtcgcag aaggtccgct
     2401 ggaagcgatt atggcggtgc ctgaatcgtt gaccgggcag tacatgagcg gtaaacgcaa
     2461 gattgaagtg ccgaagaaac gcgttccggc gaatccagaa aaagtgctga agctgacagg
     2521 cgcacgcggc aacaacctga aggacgtgac gctcacgctg ccagtcggtc tgtttacctg
     2581 catcacaggg gtttcaggtt ccggtaaatc gacgctgatt aacgacacac tgttcccgat
     2641 tgcccaacgc cagttgaatg gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca
     2701 gggactggag catttcgata aagtgatcga tatcgaccaa agcccaattg gtcgtactcc
     2761 acgttctaac ccggcgacct ataccggcgt gtttacgcct gtgcgcgaac tgtttgcggg
     2821 cgtaccggaa tcccgtgcgc gtggttatac gccaggacgt ttcagcttta acgtccgtgg
     2881 cgggcgctgc gaagcctgtc agggcgacgg tgtgatcaaa gtggagatgc acttcctgcc
     2941 ggatatctac gtgccgtgcg atcagtgcaa aggtaaacgc tataaccgtg aaacgctgga
     3001 aattaagtac aaaggcaaaa ccatccacga agtgctggat atgaccatcg aagaggcgcg
     3061 tgagttcttt gatgcggtgc cagctctggc gcgtaagctg caaacgttga tggacgttgg
     3121 tctgacgtac attcgcctgg ggcagtccgc aaccacactt tctggtggtg aagcccagcg
     3181 cgtgaagctg gcgcgtgagc tgtcaaaacg cggcaccggg cagacgctgt atattctcga
     3241 cgagccgacc accggtctgc acttcgccga tattcagcaa ctcctcgacg tgctgcataa
     3301 actgcgcgat cagggcaata ccattgtggt gattgagcac aatctcgacg tgatcaaaac
     3361 cgctgactgg attgtcgacc tgggaccaga aggcggcagt ggcggcggcg aaatcctcgt
     3421 ctccggtacg ccagaaaccg tcgcggagtg cgaagcttcg catacggcac gcttcctcaa
     3481 gccgatgctg taatcgttaa ggccgctttc tgagcggcct tttcctttca gagttgtacc
     3541 agcaatttac gcttttcttc cggtagtaaa ttcactgcct gctgataaga cgcatccacc
     3601 agataataga tttgcgaatc cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct
     3661 ttattcagat ggcggctagg acgcacatcg ctgtgctgct gacgtagcag ctccgccagc
     3721 tccgggctgg ttttcagcga aacagctggg cgattttcta cttctttcac catcgcaaac
     3781 agtacatctt ccactttgat ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg
     3841 cttggttttg ccatgcaata ttgtagcaac tccgaaatgg tcatttttta ctccccttgt
     3901 agtgtcgcga tgatgcgacg cgatccgccg tggatgcgat gttcccccag ccagatgcct
     3961 tgccaagtgc cggtctgaat acgcccttta tgtaccggca atacaagcga tgttcccagc
     4021 attgaggatt tgatatgaga aggcatatcg tctgctccct catagtcatg ctcataattt
     4081 ccgttgtcgg gaacggtgcg gaggaaaaaa cactccatgt cgtggcgtac ggtgggatcg
     4141 cagttctcat taagtgtcag agaggcggag gtatgttgca gcaacagatg cagtaagccg
     4201 atgttaacgc gcggcatatc agccagctga ttcagaattt catccgttac cagatgaaac
     4261 ccacgagatt tggcgctaag cgtgagcgtc ttttgatacc acatgtgctg ctccttgata
     4321 aaactctctt aatcagtttg cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac
     4381 agcattcggg agagcatcac tctctcccgc tctgtcagta ttctgaattg acgatcacct
     4441 cttcaccaaa cgcacccgct tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa
     4501 tgcggatacc acgagcgccg acatcgcgtg cggcggtaat atcgttatcg gaatcgccat
     4561 aaaaaattcg gatattttta tcctgcagcc attgcgattt tgtattttgc cctggtttat
     4621 cgcccgcaaa gattaccgga ttcatgttgg tggcaggaat atgaaaatta tccgccagcg
     4681 tttttgaaac cgtttctgtt ttcgtcgggc tacgaccagt cacaaagaag atcgcgtcac
     4741 cgcggcgtac atgcatatca atcagctggc gagcgacctc ttttggaatg ctgaattcat
     4801 cccagccatt attcattttt tcccagaaca caggattttt cagataatct tcgctttctg
     4861 gcgagaaggt ttttttgccg cgccagaacc ccggactgga aaaaagtacc gtgtcatcga
     4921 tatcaaaccc caccgccatt ggcggacgcc ctgcgaggct attttcaatt tgtgcgaccg
     4981 aaacccaatg aattggtgcc tgttcagcaa gcctggcaac gttagtccca gggttaagcg
     5041 gtgaaggaga tgaggccagg gcaacagcgg aactgtttag agcgaacaat aagcaaacgg
     5101 cactgattgc ctgtgtgatc ttgcgcatat ttttccctaa atagtcagta tgttgaaact
     5161 ttttattgtg agatttgttg caaaaaccat ctgaccataa cgccagctgc cggggaaagg
     5221 aaggattatc tgcggttttt gtgatgaaaa gcaggatgat tatatttaac ccagtaaata
     5281 cgttcctgtc tttttttgta taaaaaatgt ttattactcg atgtaaggat acattaattt
     5341 tacgtttatt atgatgaatc ctcggcaaat gagcgaaatt tgatagaaca tttatctgct
     5401 gtacgattta atgaacttaa tgatacagca ttacaaaaac aatcgaagtt tataaagatg
     5461 atttctgatt gaccagcccc ctaccctaca atggtgatta attttattac tcgcgatgga
     5521 gatgatgata tgaatatatc atatgtgaat agcaacaaaa cgacatcttt acctgttgag
     5581 ctagatgcac taaataataa agatatctct tatgccaaag atttcttttt atacatagaa
     5641 acacagttaa aaattgcaaa agatttttgt agacctggag aagaagtatc aagttctatt
     5701 gcaagtaaag tttttcacgc atttattgat ttagttaaca aaatcagggg taagaaagac
     5761 tttatgtata tttgcacgct ttgctgtttt gccgaggagg ttaaaggtga ttattctcat
     5821 tacaggacct ttttatttga tattggtaat caatataagg ttaaacttac acaaagcgga
     5881 aaaaagagtt ctctttaaca ttagaattta acgatactat aattgaatct cagaaagtca
     5941 caggcaataa ggcaaagcat attcttgagg atatagaaat attctatcgt aacaaacccg
     6001 atacttatta ttaaatttaa taatggttag atgtgcagaa attattactc cacacgaaaa
     6061 aatagggaaa ggaattccag ataattggga taaacttaac aataaaagaa ctgacaacgt
     6121 tttattatct ctcacgtaga acaacggcat caaaaaaatc atctacaaag aaatagtgaa
     6181 agcgacgaca gtttaatttc actgcaggct gggtaactcc agcctgcttt cctgcattac
     6241 atcaccgcag caaacgcctt tgccacacgt tgtacatttg ccgtatttaa cccggcgaca
     6301 cacatgcgac cgctggcgat gagatagaca ccaaattctt cacgtagtcg gtcaacctga
     6361 gcggcactta aaccggtata actgaacatg ccgcgctgat taagcagata atcgaaattg
     6421 cgttctggca tctctgtgct taataccttc accaattcct gacgcattgc cagaatgcga
     6481 gtacgcatct cttctacttc cgccagccag ctggctttca atgcctcgtc attcagcact
     6541 gcagccacca cctgcgcacc aaaattcggc gggctggagt agttgcggcg aactgttgct
     6601 ttcaattgcc ccagtacgcg gccagcggct tcggcatctt cacacagaac agaaagtccg
     6661 ccgacgcgct cgccgtaaag ggagaaaatt ttcgagaacg aattgctcac cagagcgggt
     6721 aatccagcac tggcaatggc gcgaatagcg taggcatcct cttccatacc ggcactaaat
     6781 ccttgatagg caatatcgag gaatgggata agctcgcggg ctttgagaat ttcaatcacc
     6841 gcatcccact gatcattagt gagatcggca cccgttgggt tgtggcaaca tggatgcagc
     6901 aacacaatac tgcgggcagg taatgttttc agcgtcgcca acaggtcatt aaagcgcacg
     6961 ctgttagtca cttcgtcata ccaggggtaa gtactcactt cgaatccagc cccggcgaat
     7021 attgctacgt ggttttccca agtaggatcg ctgacccaga cgcctgattc cgggaagtag
     7081 cgtttcagga aatccgcgcc cactttcaat gcccctgagc cgccaagggt ttgaatggtt
     7141 gctacgcgct gttgttgcag taccggatgg tccgcaccaa acagcagcgg cgcaatggca
     7201 tggcgatagc tgttaagccc ttccatcggt aaataaagcg aagcgccatg gggctgcgca
     7261 ttcaggcgcg cttccgcctc cgccacggct ttcaattgtg gaataattcc gtcttcgttg
     7321 tagtacagac cgatacttaa attcactttg tcgctgcgag ggtcttcttt aaaacgctcc
     7381 ataagcgtaa gaatcgggtc gccagcgtag gcgtcaactt tttgaaacac gcgatggttc
     7441 tccaggttta cgggcaggtg gttaaaacac aataaaccgg aagaaggcga agatcgagtg
     7501 gatgttcagg agcgaacggc aattagcaac agagtgagac taatgataaa cgtacatccg
     7561 ccagaggcac gacctttata agagcatgaa agaacatcaa cttattgaat ttttaggatt
     7621 ttattggccg gataaggcat tcacgccgca tccggcacag acaatcaaat attacagaac
     7681 gattaatcca cgtatttcat cgcgaccctt gaagtcaggc gcgtaataag ttcgtaagcg
     7741 cttactttcg tcatttcagc gatacgttct acgggcaaac cttcgcccca taaaatgacc
     7801 ggatccccgg ctttgtcctg cgcctgtgga cctaagtcta cgcagatcat atccatcgcc
     7861 acgcgcccga caatcggtac ttcgcgaccg ttcaccagca ctggcgtacc ggacggcgcg
     7921 gcgcgcggat aaccatcgcc atagcccatc gcgactacgc caagacgggt atcacgttcg
     7981 cttacccagg ttccaccata accaacaggc tctccggctt tatgctcacg cacggcaatc
     8041 aggctggagg ttagtgacat cactggctga cagccaaaat cggcaccggt ggagcgatct
     8101 tccagcggcg agacgccata aagaatgatg cccgggcgca cccagtcaaa atgcgactgt
     8161 ggccacagca gaatgccacc cgacgcggca atggaacgtt gaccaggttt gccttcgcaa
     8221 aaggtattaa agatagcgag ttgtttctcg gttgcgccac attttggttc atccgcgcgc
     8281 gcaaaatggc tgacgatatt caccggctga cgaacgtttt tgcactgggt caggcgatga
     8341 taaaacgcct cagcctgttc cggccttacg cccagacggt gcataccggt atcgagtttc
     8401 atccagacgg taaccggctc gtccaagcta gcctcttcca gcgcagccag ctgttcttcg
     8461 ttatgcacgg cggtatgaaa atgttgcgca gaaatcgtcg gcagatctct ggcatcaaaa
     8521 aagccttcga gtaacagtac aggtttggtg attccccccg cacgcagtcg cagagcttct
     8581 tcgagacggg ctacgccaaa ggcgtcagca tcggggagcg ttcgcgcggt ctcaagaaga
     8641 ccgtgaccat aagcgttcgc tttcaccacc gcaaccattt tactggcagg tgccagttca
     8701 cgaagacgtt gcaggttgtg tcgcagagcg cggcggttaa tcacaacagt tgccgcttgc
     8761 atttgtgttc cttgataatt gtttgcttta attacctaat tcataaaata attattattc
     8821 gtcgtcgtac tgcggccccg catagttgtc gaagcgcgac cattgaccgt taaaggtcag
     8881 gcgtaccgtc ccgattgggc cgttacgttg tttaccgata ataatttccg cgatgccttt
     8941 taaatcactg ttttcgtgat acacctcatc acgatagata aacatgatca agtccgcatc
     9001 ctgctcgata gagccagatt cacgcaggtc ggagttgacc ggacgtttgt cggcacgttg
     9061 ttccagagaa cggttcaact gggacagcgc caccaccggc acgttcagtt ctttcgccag
     9121 tgctttcagc gagcgggaga tttctgcaat ttccagcgta cggttatcgg aaagcgccgg
     9181 tacgcgcatc agttgcaggt agtcgatcat gataagcccg atgccgccgt gttcacgggc
     9241 aatacggcgt gcgcgggaac gcacttccgt tggcgtcagg ccggaagagt catcgatata
     9301 gatattgcgt ttttcgagca aaatacccat ggtgccggaa atgcgcgccc agtcttcatc
     9361 atcgagctgc ccggtacgga ttttagtctg gtcaacgcgc gacagcgacg ccagagaacg
     9421 catcatgatc tgttctgatg gcatctccag cgagaagata agtactggtt tatcctgcaa
     9481 cattgccgcg ttttcgacga ggttcatcgc aaatgttgtt ttacccatcg acggacgcgc
     9541 ggcgacgatg atcaaatccg acggctgcaa gccagcggtt tttttgttga gatcgtcata
     9601 accggtgttt accccggtaa cgccatcgtg tggctgctga aacaactgct caatacgcgc
     9661 cacggttgcg tcgagcacat cggcgatgtt cttcggccct tcgtctttgt ttgcacgact
     9721 ttcggcaatt ttaaagacgc gggattcagc aaggtccagc agatcttcgc tggtacgccc
     9781 ctgcggatca aaaccggctt cagcaatctc attcgcaacc gagatcatct cacggacaac
     9841 ggcacgttca cgcacgatgt ccgcataagc actgatgttc gccgcacttg gcgtattttt
     9901 tgacagctct gccagataag caaaaccacc gacgctatcg agttgcccct ggcgttccaa
     9961 cgattccgca agggtaatca gatcgatagg actaccgctt tcctgcaaac gcgccatttc
    10021 agtaaagata tgacggtgtg ggcgggtgta aaaatcgtct gctaccacac gctcggctac
    10081 atcatcccag cgttcgttat ccagcattaa accgcccaac accgactgct ccgcttcgat
    10141 cgagtgcgga ggcactttca gcccggcaac ttgtggatcg cgttcgcggg gttcagcctg
    10201 ctgtttgttg aagggtttat ttcctgccat agtgaatgga gttaccgaaa taaagaatgg
    10261 gtcgaaactt taccatatga agcagaccct gacgatacgt tctggaggac acatggcaac
    10321 acgaattgaa tttcacaagc acggtggccc ggaagtactt caagccgtag agttcactcc
    10381 tgccgatccg gcggagaatg aaatccaggt cgaaaataaa gccatcggca tcaattttat
    10441 cgacacgtat atccgcagcg gcctttaccc gccgccatcg ctacccagcg gattaggtac
    10501 cgaagcggca ggcatcgtga gtaaagtcgg cagtggtgta aagcatatta aggctggcga
    10561 tcatgtagtc tatgcgcagt cggcgttagg cgcttacagc tctgtgcata acattaatgc
    10621 ggataaagcg gcgattctgc ctgcggcaat ttcttttgag caagctgcgg catccttcct
    10681 gaaaggctta acggtttatt atctgctgcg caaaacctat gaaattaaac ccgatgaaca
    10741 gttcctgttc cacgcagcgg ctggcggcat tggcttaatt gcctgccagt gggcaaaagc
    10801 cctgggcgcg aaactcatcg gcaccgtagg aaccgcgcaa aaagcgcaga gcgcgctaaa
    10861 agccggcgcg tggcaggtta ttaactatcg tgaagagaat ctggtcgagc gattgaaaga
    10921 gatcaccggc ggtaagaaag tgcgcgtagt gtacgattcc gtgggcagag acacctggga
    10981 acggtcgctg gattgcctgc aacgccgcgg cttaatggtc agttttggca actcatcagg
    11041 tgcggttacc ggtgtgaact taggcattct caatcaaaaa ggctcgttgt atgtgacacg
    11101 cccttccctg caaggctata tcaccacgcg ggaggaatta accgaggcca gtaatgaact
    11161 gttctctttg attgccagcg gtgtgattaa ggtcgatgtc gccgagcagc agaaatatcc
    11221 gctgaaggat gcgcagcgtg cgcatgagat tctggaaagc cgggcgacgc aaggttccag
    11281 cctgctgatt ccataaaaga aatagggctt ccacctggga agccctttct ttttatagtt
    11341 cggctgtatg tagggtacag cacgatgaat ctgttagagg cgcaatagtg acagatttga
    11401 ttatcaattc ctattttgtt ctaaggataa aaccttaggt tgtgatcatc cgcacaatcc
    11461 cttagtaacg ccagcggtca taacgctgat atttcggcat ttttggtgct ttaatcgcct
    11521 taataaccca caccaccgca atcgccagta gtaaccacgg cagcaactta atcatcaatg
    11581 ccagcatacc gccgaggaac ataatggccg tcgccacaac cagcgcggcg ataatgccca
    11641 gcaacgaaac gccggtgacc atcagcatga caaaaaagcc aatcacaaaa agtagttcca
    11701 gcatgatgct ctcccaaata tgaaatctct tgctggcatt acaagaatca tgccaaaaat
    11761 aatctattga tttaacagca aaacgccccg cgacggtgcg cagggcgtgg tgaatttgac
    11821 tactttttgg tgaaaagtta acgcttatcc gccaccagtt tgagcgcgtg ttccagcacg
    11881 ttaatgtcag cacccgcttt atgggcattt tcacttaaat aacgccgcca ctgccgcgcg
    11941 ccaggaatac cctggaacaa acccagcata tgccggataa tatggccgag atacgtcccc
    12001 tggctgagtt catgctcaat gtacggatac atggcacgca ctactgccac cggatcggca
    12061 tcggtatccg aggaaccaaa gatctcccgg tctaccgccg ccagaatacc cggattctga
    12121 tacgcctcgc gcccgaccat cacgccatcc atatgttgca aatgcgcttt agcttcttcc
    12181 agcgacttga taccaccgtt aatcgacatt gtcagatgcg ggaagtcacg cttcagttga
    12241 tacacacgcg gataatcgag cggcgggatt tcacggtttt ctttcgggct taaccccgaa
    12301 agccaggctt tacgtgcgtg gatgatgaac atctcacact cgcctttgcc ggaaacggta
    12361 ttgatgaaat cgcagagaaa ttcatagctg tcctggtcgt cgatgccaat acgcgttttc
    12421 accgtcaccg gaatcgacac cacatcgcgc atcgctttca cgcagtcggc aaccagctgc
    12481 gcattaccca tcagacacgc accaaacatg ccgttctgca cccggtcaga cgggcagccg
    12541 acattcaggt tgatctcatc atatccgcgc gcttctgcca gctttgcaca ctgcgccagc
    12601 gccgccggat cgctaccgcc cagttgcaac gctaccggat gttcttcttc actgtacgcc
    12661 agataatcac ctttaccgtg aattatcgcc cctgtggtca ccatttcggt ataaagcaac
    12721 gtattgcggg aaagcagacg caagaaatag cggcaatgtc tgtccgtcca gtcgagcatt
    12781 ggtgcaacac taaaccgacc actccagtga acgtcagttt tttcaggcat tgcgctggtt
    12841 tggttgattt tttgcatttc agaattaccg tgcattttca aatgtagaga ttattttatc
    12901 gatatatcat ggggttatgt tattcagctt cactgttcag gaggctcaat agcggggtac
    12961 tataccataa caacaggaag cgcctgtctc attgcaaaag aaaattgaga tcctctcaag
    13021 gcatgaagct ctcacgaagt gatggaaata atcttattag ccgttaactt tgttaaggcc
    13081 aatgataaac aatccaggtt cgacgataaa taaaaaatca cacattaaac tctggtgata
    13141 tatctccctg ctaatagcat tgatagagaa aaaagaaccc aataaatatt gggtcctttt
    13201 atataatgcc ctccatacta tcgaagaact tcacatatta tttctccgat ttaaccccga
    13261 acaagtcata aattaattta gaagtgtctg taagtatttt aatctcttcc tttgaggttg
    13321 ggtcaaacga cttcgcgaag ctaattaatt gtggtgcagc atctcgcatt ttgcttaaaa
    13381 tatcaggatc gagcgttcct tcattcacca gatgtgacat cttatccaga tagccatcaa
    13441 aattcatttc tcccccatct gccagacgct tcatctctcc caggtacttc ttcatatcac
    13501 gttgatataa tttttcaaac tgagctttca ggtagctctc attattttca ttaatataca
    13561 ctgtttctga taaatctgcg taggcactag aatatgaaag tgaaattaac agcgaagcaa
    13621 ataattttaa gctgttgtca tcatgttcct ggcaggcatt aacaaacgtt aaaaaaccag
    13681 aacctattgc ttgaaaatgt atattcgcta aaggttcatt atccttagat tcctcataaa
    13741 atagctgaac cgtggaggga aggccattct tagtttctac atcaaaagta cattgttcaa
    13801 tgggcattgc atctttatta tccccaggat tacatatact attaatcgcg aaatgaaaaa
    13861 tagcccggtc aacaactgat gcaataaagt cacgatcgtt taaatcctca tcagtgcaat
    13921 cgtcagtata cttattgacc cacattttga gatcatttat gtgctcaatt aaaaacacat
    13981 ttttttacgt gcatgccata aattatgcgt gattcgttga ataaacaaat ctatttttgc
    14041 aataattata tcccgatgac gcagcgctgc ataatcaata gatttaccat atatattatt
    14101 taatttacct ttgattgaac ctctattatc caatttatct ttggttgcat ctttattttc
    14161 gaattgattt gaattgagtg ttttattaaa gtatccagtt gcaccgggta ttatcttcaa
    14221 catgattcat ctaccttttt tgtcgtaccg tgtcctcctt ataattcttt tgccatctgg
    14281 tcactatcaa aaatcgctca taatttctga actacaggta tgcgtttaaa cccagttaaa
    14341 taaatagcga gttaacttga agcaatttat ttaaaaatga attattaatt attatcgtgt
    14401 tgataatggt acgataaggc aacacaacac cttacctaca aaacagaaaa tcaacattac
    14461 ggcaacaata agggttctcg gtgtttttcc tggaacatgg taaagtaagg acattcttaa
    14521 cccccacttt gaggtgcccg atggaaaaga ccacaacgca ggagttatta gcgcaggctg
    14581 aaaaaatctg cgcgcagcgt aatgtgcgcc tgaccccaca gcgcctggaa gtgttgcgcc
    14641 tgatgagtct gcaggatggc gctatcagcg cttatgatct gcttgattta ctgcgcgaag
    14701 ctgaaccgca agccaagccg ccaacggttt atcgcgcgct ggattttctg cttgagcaag
    14761 gttttgtgca taaggtggaa tccaccaaca gttatgtgct ctgtcatctg ttcgaccagc
    14821 ccacgcatac gtcagccatg tttatttgcg atcgctgcgg cgcggtgaaa gaagagtgtg
    14881 cagaaggcgt ggaagacatt atgcatacgc tggcggcaaa aatggggttt gccctgcggc
    14941 ataatgtgat tgaagcacat gggctctgtg cggcatgcgt ggaagtggaa gcgtgtcgtc
    15001 atcctgaaca gtgccagcat gatcactctg tgcaggtgaa aaagaaaccg cgttaagagg
    15061 gcgtacatcc ttgtacacgt cgggcaggag ggattaatta ccagcgatat tcattgcggg
    15121 tttcccaatc cacgacctct ttttctgcct gatctttctg ataaccataa cgttcctgga
    15181 ttttaccgac cagttgatca cgtttacctt caatgatcgt catatcatca tcggtcagtt
    15241 tgccccattg ctctttcact ttacctttaa actgtttcca gttaccgccg gcttcatctt
    15301 tattcataat caagacctca tcgttaggtt gtgaatgaga gtacgttcac ttttcttctg
    15361 aacgtgagat taagtgtagt cagcaatttg gcttaggata atttattcag aatttttaac
    15421 cgtcacgttg cggcaaacca ggtaccgttg cgccagtgac gccgccagat agccgccaga
    15481 gaaagcccgc gcaacgccag aaagacggtt aatgccagcc acaaaccatg attacccagc
    15541 cacggcagcg taaggagcgt cagcgcaaaa cctgcggcgg ccaccgccat actgttacgc
    15601 atttcggcgg cacgcgttgc gcctataaac atgccgtcca gcagataaca ccagacgcca
    15661 accaacggca aaatcacctg ccagataaga tagcggtcag ccagctgctg aatctgggtt
    15721 aacgacgtca gcaacgcaat gatgtgttcc cctgccagca aataaaccac cgaaaatagt
    15781 aacgctacaa tccccgactg gcggcacgct gcccgccata catccagcaa ctggctaccg
    15841 tcgcgcgcac cgtatgcctg accggagtgc gcttcaaccg cgtaggcaaa accatcaagc
    15901 gcataggcgg taaaggtgag tagcgtcatc agaaccgcgt taacagcgat aatgtcactc
    15961 cccagtcgcg caccaagtac ggtgatcgcg ccgaaacaga gttgcaacaa cagcgaacgc
    16021 agcatgatat cgcggttaag cgccagcaag cgacggaagt ttcctcgcca ggcagttttc
    16081 agcatttcgc cggaaattcc gcgtagtttg aggattttac gcaccattag cagaccaatc
    16141 agcaatgttg catattccgc aataaccgtc gccagcgccg cgccctgcac gttcatatgc
    16201 agccccatca ccagccagac atccagcaca atgttgagga tattgccgac cactaacaaa
    16261 attactggcg cacgggcata ttgcacgccg agtaaccaac cgagtaatac cagattcgcc
    16321 agcgacgccg gagcgcttaa ccagcggatt tcaagaaagc gccgcgcctg ttccaggacc
    16381 gcttcactac cgccaacaat atgcagcgcc agatcgataa tcggcgtacg caacagcgca
    16441 attaacgccc cggcccccaa cgccaacagc aacggttgca ccagcgcacg ggctaatgcc
    16501 tgaggatttt tggcaccata agcctgcgca gtcagcccgg tggtgctcat gcgtaaaaac
    16561 agcaacagca taaagagaaa gctggtcgcc gttgcaccaa ccgccacgcc gcccaaataa
    16621 accggactat caagatgacc aattaccgcc gtatcgacca gtcccagcaa cggaacggtg
    16681 atattggaga aaatcatggg taaggcgaga tgccagagtg ctttatcaga tgaagtgagg
    16741 aatgccatgc agacaagcct gatgaagaga gatgaaaaac aaaccgcgac gccaagcggc
    16801 atcgcggtct cagagatatg ttacagccag tcgccgttgc gaataacccc aaccgccagc
    16861 ccttcaatgg tgaagctctg ctgacgaagg tcaacgacaa ttggtttaaa ctcgctattt
    16921 tctggcaaca gttcgacttt attgccctgt tttttcaggc gcttaacggt aacttcgtca
    16981 tcaatacgtg cgacaacgac ctgaccgtta cgtacatcct gagttttatg cactgccagc
    17041 aagtcgccat ccataatgcc gatatctttc atcgacatcc cgctgacgcg cagcaggaaa
    17101 tcagcattcg gcttgaacaa ggaaggatcg acctgataat gaccttcaat atgctgttgc
    17161 gccagaagcg gttcaccggc agccacacga cctaccagcg gcaacccttc ttcctcttcc
    17221 tgcaacagac gaatcccgcg tgatgcgccg gaaacaattt caataacgcc tttgcgtgcc
    17281 agcgccttca gatgttcttc agccgcgttt ggggaacgga accccaaacg ctgcgcgatt
    17341 tccgcacgcg tcggcggcat acctgtctgg ctgatgtgat cacggatgag atcaaacacc
    17401 tcttgttgcc tggccgttaa cgctttcatt ccgccccctg ggtgtatata cagttatact
    17461 gtgagtatat acagcaaaag gcgattttgg aaccataaac tgcacaataa accagagatt
    17521 tatcgaaatc tggaagggtt atccaaaatg cgaccataac aggatgcacc aggtaatcac
    17581 ggcgacgata atggcaatca gcaccgcggc ggatcccata tcttttgcgc gtccggaaag
    17641 ctcatggtat tcagagccaa ttcggtcaac cactgcttcg atggcgctat tgaggatttc
    17701 cacaatcatc accagcatca cggagctgat aagcagcacg cgggtaattg cgtccacatc
    17761 cagccagcag gcgatgacca ccgccaacaa taccgctacg ccttcctgac ggaatgccgc
    17821 ttcgttgatc catgcagcgc gtaaaccttt ccaggaatag ccagcagctt tgataattcg
    17881 ggtgaatcca gttgtattat tggccattga aagaaccttt ttacattatg agcgtcaata
    17941 tcagtgtacc gttaaatccg atgattcggg tttgcgtaac gccgcgcgaa acatgagcgg
    18001 ataccacaga atttcccatg actttctgct atccttgccg cgcatttgca ttattaacca
    18061 gaggctttac atcgtttatg tccggctggc cacgaattta ctacaaatta ctgaatttac
    18121 cattaagcat cctggtaaaa agcaagtcta ttccggcaga tcctgccccg gaactggggc
    18181 tggatacctc tcgtccaatt atgtacgttt taccgtacaa ctcgaaagca gatttgctga
    18241 cgttgcgcgc ccagtgtctg gcacatgatt tgcctgaccc gttagagccg ctggaaatcg
    18301 acggcacgct actgccgcgc tatgtgttca ttcacggcgg gccgcgtgta ttcacctatt
    18361 acacgccgaa agaagagtct attaagcttt tccacgacta tctcgatttg caccgtagca
    18421 acccaaatct ggatgtgcag atggtgccag tatcggtgat gtttggtcgc gcgccggggc
    18481 gtgaaaaagg cgaagtgaac ccgccgctgc gtatgcttaa cggcgtacag aaatttttcg
    18541 ctgtactgtg gctcggtcgc gacagttttg tgcgtttctc gccgtcagtg tcgctgcgcc
    18601 gtatggcgga tgaacacggc acggataaaa ctatcgctca gaaactggcg cgcgtggcgc
    18661 gtatgcactt tgcccgtcaa cgtctggctg ccgtaggccc acgtctgcct gctcgtcagg
    18721 atctgtttaa taagctgctc gcctcccgcg ccattgccaa agcggtagaa gatgaagcgc
    18781 gcagcaaaaa aatctcccat gaaaaagcgc agcagaacgc gattgcgctg atggaagaga
    18841 ttgcggcgaa tttctcttac gagatgatcc gcctgactga ccgcattctg ggcttcacct
    18901 ggaaccgatt ttatcagggc atcaacgtcc ataacgccga gcgcgttcgc cagctggccc
    18961 acgacggtca tgagctggtg tatgtgcctt gccaccgcag tcacatggac tacctgctac
    19021 tttcttacgt gctgtaccac cagggactgg tgccgccgca tatcgccgcc gggatcaacc
    19081 tgaacttctg gccggccggg ccgattttcc gccgtctggg ggcgttcttt attcgccgta
    19141 cttttaaagg caataaactt tattccaccg ttttccgtga gtatctcggc gaactgttca
    19201 gccgtggtta ttccgtcgag tacttcgtgg aaggcggtcg ttcccgcacg gggcgtttgc
    19261 tggatccgaa aaccggtacg ctgtcgatga ccattcaggc gatgctgcgt ggcggcacgc
    19321 gtccgattac gctgattccg atctatatcg gttatgagca cgtcatggaa gtgggtactt
    19381 acgccaaaga actgcgcggc gcgacgaaag agaaagagag cctgccgcag atgctgcgcg
    19441 gtttaagcaa gctgcgtaat ctcggtcagg gttacgtcaa cttcggtgaa ccaatgccgt
    19501 tgatgaccta ccttaaccag cacgtaccag actggcgtga atctatcgat cccatcgaag
    19561 cggtgcgtcc ggcctggtta acgccgacgg tcaataatat tgctgccgat ctgatggtac
    19621 gcattaacaa cgcaggcgcg gcaaacgcca tgaacctgtg ctgtactgcg ctactggcat
    19681 cacgtcagcg ctcactcacc cgcgagcagt taaccgagca actcaactgc tacctggatc
    19741 tgatgcgcaa cgtaccttac tccacggact ctaccgttcc ttcagccagc gccagcgagc
    19801 ttatcgatca cgcgctgcaa atgaacaagt ttgaagtcga gaaagacact atcggcgaca
    19861 tcatcattct gccacgcgag caagcggtgc tgatgaccta ctatcgcaac aacattgcgc
    19921 atatgttggt gctgccttcg ctgatggcgg caattgtcac ccagcatcgc cacatctccc
    19981 gcgacgtatt gatggagcac gtcaatgtgc tttacccaat gctgaaagcg gagctgttcc
    20041 tgcgctggga tcgcgacgag ttgccggacg ttattgatgc gctggcaaat gagatgcaac
    20101 gtcaggggct gattaccctg caagatgatg agttgcatat caacccggcg cattctcgca
    20161 ccctacagct gctggccgca ggcgcgcgcg aaacgctgca acgttatgcc atcaccttct
    20221 ggttgttgag tgccaacccg tcgatcaacc gcggtacgct ggagaaagag agccgcaccg
    20281 ttgcgcaacg tctctccgtg ctgcacggca tcaacgcgcc ggagttcttc gacaaggcgg
    20341 tgttcagttc tctggtgctg acgctgcgtg atgaagggta tatcagcgat agcggcgatg
    20401 ccgaaccagc agaaacgatg aaggtttatc agttgctggc ggagttgatt acatcagacg
    20461 tgcgtttgac gattgagagt gcgacgcagg gcgaagggta atcagagaga attgccggat
    20521 gcggcgaaaa cgccttatcc ggcctaccat gacctgcaaa ttcaataaat tgcgattcac
    20581 cgggtaggcc tgagaagcgc aacgcatcag gcaacccaga agaaagccgg atgatcatcc
    20641 ggctttttta catcatcaga aatgccagta actcattgcc agtcctaaaa acagtaccag
    20701 accaacatag ttattattca taaatgcttt aaagcaggct tcacgctcgc ggttggcaat
    20761 cagtttttgt tgataaacaa acagcgcgcc agccaccaca attgaccagt aatatcccca
    20821 gcctaagcca tttaactcac cgatgatcgc catcagtgcc agtacgccaa tctgcaaaat
    20881 accaataatc aatttatcgt attggccgaa caggattgcc gtggatttaa tgccaatctt
    20941 cacatcatca tcgcggtcaa ccatcgcata ctgcgtgtcg taagccaccg cccagagaat
    21001 attggcgagg aacattaacc agcaactcaa tggcaccgac tcactcacag cggcaaaagc
    21061 cattggaatc gaccagccaa acgccgcgcc cagcaccact tgcgggagat gggtataccg
    21121 cttcataaac gggtacaccc acgccaatgc cagagcggca atcgacaaca gaatggtcat
    21181 cgtattcagc gtcagaacca gtaaaaacga aatcagtacc agcactacaa acagcgcgcg
    21241 cgcctctttc tctgttaccg cgccgctggg aagtggtcgg ttcgccgtgc gcttaacatg
    21301 accatcaaac ttgcgatcag cataatcatt caccacgcaa ccagcagcgc gcatcagcca
    21361 gacacccgcg acaaacaccg ccatgatcca gagctgagga acgcccggtg tcgccaccca
    21421 caacgcccat aatgttggcc agagcagcag taacgcgcca attggcttat ccgtacgcat
    21481 taagcgatga aacgccagca gcttattctg cgtcagactc cactccatat ttttttcctc
    21541 ttagtacaac ggtgacgccg gtaaaaacag ttctgttagc aacagcggtt taccgcttaa
    21601 tcgcaggcgg gaacgtcgcc cccacagtcc ggcatcacgg cctatctcga taaaatcccg
    21661 ggttagtgtc gatgatgtga acagatagcg tcctaacggc gttttaccca atttttgtaa
    21721 cgctagttcc ggcccgctta atgttgatac aggaacgacg gtacgaccgg caagccacgg
    21781 ttcaccatcg gcacataaca aaatttcacg taaccagtaa cgagactctt tcggcagcag
    21841 cggcagttct tcggggattt cattctgctc gacaaacccc tcgcggatca tcgtcacact
    21901 caccgttttt ccctgctgtt caaaacgttt tgtcatggaa tcctccagca acagccagtc
    21961 gagcagttgc ggatccaggg cagggatctc tttaaaatag cgcagcgcac gcagttgcgt
    22021 taacgcgggg tgtgacatgc cgaactctcc gttacataac gtaaaggcat tgtatcgcag
    22081 aaaagtgttt acgggagatg taaagcaaat taagtgatct aatgcgcaac aaaaacgcaa
    22141 caatcgcatg aagtggtaaa acgctgttga accttacgct ttgccgatgc ccgtgctggc
    22201 gagggcaacc ataggggcat caccgcccat gatgctagga acggtgatgt tttcctttac
    22261 ttaaatacct ttattcttta tacgagtagc tcgccaactc gcagtaaaaa ttgggaaaag
    22321 cgcatattta ccatgagatt tggagctgaa aagtgtttcc tgagtaagat taataggcaa
    22381 catcttatgt ctggatttat agatacacgt ggaattccaa ttggtatctt ttgtaggatt
    22441 caacatatca tgtaagcgct gggagcttac caccataaca ggcccatcat cgacagagga
    22501 gaacaggata aatatattat cgtttcgtgt taatttatta gtgttaaagg ttcttaactt
    22561 ttcttcagtt tctataaata taggtgaaac atcatcacta cgaatccact ctttataaag
    22621 ataccaggca tttgctttga tcatatcatc cgttgatcgt aaggcctgat cgataagttg
    22681 gataaaaaaa ttgttatttt tatcaaccag ccctttattc atattaaatc tgttaataaa
    22741 atggatcatc tgttgggtat cgaaattcac cttactgaat cgatgttgac gcataaaatt
    22801 atcgaacttg tcatcttccc cagaatgtat ataactacgt ataattggat cttttatata
    22861 aggacaatgg ctaaatgttt tcttcatatt ctcaataaag tttttattaa caatattgag
    22921 aatttttaat tgcatcacca gttttactgc cagaacggat ttcaatatca ttgcattatc
    22981 tggcaatgta tcaatcattt ttataatgtt attgatataa ccatcgagat cttcattccc
    23041 aaatatatct gaataatcaa tagttaattc caattgttct gtaatgcaat cttaaaaatt
    23101 ccctgtcgac attcgcacat gatcaaacat aacagctgtc aaattcgatt taataaatga
    23161 tatttctttg gcagacagaa actctgctct ggtgaagtgc gaagcgtaaa gataacaatt
    23221 atcgaagttt gaatttctaa tctgacattc gcgaaaatta ctatgaccaa gttgactctg
    23281 cgagaagtca actgtatcaa gaatattttg gctaaaatca acacattcgc cactcgtttt
    23341 gagaaaaatc acattgtgta aacgtcgaca tttaaatatg acattatcaa ctgttgcctt
    23401 agtaaaattg caattctcaa tttctttaca ttcgaaagtt acatgttgta gatttgatga
    23461 taaaaaagac accttagaaa taacgtcacc tttaaaatga caatttgtta aatcaaaatc
    23521 agacaggtcg atatacccct gcgcatcttt atccatcaga aaattacaag cgcattctat
    23581 tttttcattt ctgtagaatt ttacacttat agtatcatta ctaattgaga cttcacgtaa
    23641 catatttatt gcatgatttt tttcgttccg ttgttttata tttaaaaata tatcactgat
    23701 ataaataaag taatctctgt ttaaacctgt caccccattg aaattacgta gtaaatcaag
    23761 atctactata ttatttcctt gcaccacatc atgtttcatc aaagagttat tgccgccaga
    23821 atttgtgata tgacctatca taaaacctct gtaatacaga aataaaataa cctggaaaag
    23881 tagcctgagc gtccagacac taccggcgaa aggaaaagaa tctctgataa tgcattgaaa
    23941 taatggacat tcttagtgaa gaagaagtga agtactataa aaaatcgctc atatcccagg
    24001 aaggaagcat aaaaaaaggt gcgccaggag acgcaccagt tgttgcaaaa tcagcactgt
    24061 ggggcgtaat taccccttgc cttttacact gctgataaag gtggaacggg cagatgtcga
    24121 tcccaggcgt tcagcttcat caagcagttt taacgcctta tcaacatcac ctttcgcgac
    24181 agcgtttttg atcgcggtat taaaataact ttccgtgtcg ttgagcatcg gctcgctttt
    24241 cttcaccggc accggagcag gtgcagccac agctggtgcc gccgtgttgc ctaccgtaac
    24301 cggagctgga gcggaagaac caaatagcgg tcctaccaac acgctggagc tggagttcgt
    24361 tttgactttc agtttcagta agccatcgct ggtatgacga gcaaccggat cgggaatatc
    24421 cgggatcgag ttaccgacgc ctttggcata ggctttagcc gggtcgagca gttgggtcgt
    24481 ctgctggaga tctttttccg tggtaaagac cagaacataa agtttttgct gccccaacgc
    24541 cggtgtcagg cgcataacgc cttccagccg atctgcactc atcacgccta gttcctggta
    24601 ggtgaaataa ctgctgggga agaaggctga tggggtcatg ttctgatcaa gaatcagcac
    24661 gttcggcgca aaaacgctgg tttgtttgtt cacttcgctg gtcagcgtca gggtcagttc
    24721 gccaatgttt gccgggacgc tgtacgcagc aaccggacca ctgatgccgg gaacgttcag
    24781 ttgttggccg ccggtcgcca gttgggtggt ctgggtttta gattgatcga ccggtgtcca
    24841 ggtgagttgt tgcagcgcgg cagatggaat ggctggcgcg tcgctggtgt tttgcggtac
    24901 gtagttaaca tcggcaaggc taattccagg cgcgcttgcc agtaaccctg ctgataaaca
    24961 gagggcgatg agacttttat tcattttcat tgttttcacc tcaaaatctg gagctcagcg
    25021 gtagccaggc aatagcgcgc taaacccgat aatcagaggg gctttcgccc ctttcagata
    25081 atgacaacct gtttttatgc cggatgcggc gtaaacgcct tatccggccc aggttttgct
    25141 attaccacca gatttccatc tgggcaccga aggtccactc gtcgctgtcg ccacgaccga
    25201 agctgccgcc gttgaaatca gcaggaacgg ctttgccgta gttcgggtta accttgctat
    25261 cgccgttgta gtcgtaaccc catttctcat cccacttggc gtaggttgcg aagacacgaa
    25321 tagccgggcg tgaccagatg ctgtcgccag cctgccattg ttgtgcgagg gtaattttgt
    25381 actgattgtt cttgtcgccg gtgcgctggg attcgacgtt gtcgtagccg atttccatca
    25441 cggtgctcat gattggcgtc cacttgtaca tcgggcgaat accaacggtc caccacttgg
    25501 tgccgttgtc gttatcccag ttgatatcct ggtacatacc cacgtacatc atgtcccagt
    25561 tgtcgcccat ggagatcgca ccgtggtcga ggatacgcag catgtgaccg ttgttgttga
    25621 tattgtaggc aaatttttcg ttatcaaacg cgacgccaga accctgcgac agacctttac
    25681 cctgcgaggt catcgagtca gtagcgtact gaacaacaaa cttgttaaag cccttcagga
    25741 cactctgagt atgttcagca gtgaataacc agccgtcttt cgatgcgcca tcaaccagac
    25801 gatagttatc acgcaggttg gcacgaccgt agtcgacacc cagttctaat gtgccgcccg
    25861 ggttgatttc catctgcgct aaacgcacat cgaaaacgtc gttcgcggtt tcgttggtat
    25921 agtcataaat attgttgctg gcaaaagagg aagaaccacc agcttcagag gagcgggttg
    25981 ctgccagaga gagtttaccg aagccaacat cgatgttttc cagaccggca ccaggaccag
    26041 aaatatccca gtagtagaag tcgatcatat gaacgtcatg acgttggtag aagcgcttac
    26101 ctgcccagat ggtggaacct ggcagccatt cgatcaggtt ttttccctgc acgtttgctt
    26161 cacggaaggc cggatcggta gcttcccagt cattctgttg cgcgacggaa taggccacgt
    26221 tagtgtcgaa atagaagctc ttatcgccct ctttccacac ttcctgaccc aattttaatt
    26281 cagcataagt ttcacattcg ttgccaagac ggtatttact ttgagcaccg gtagtctgga
    26341 aacactgttg ttcaccgccg ctacctgtcc agccaatacc ggaacgtgca tagccgtgga
    26401 aatcaacagc cattgcctga gcagacatta cgcccgctgc gacggcaacc gccagaggaa
    26461 gtttgcgcag agtaatcatc attctatctc ctgagtcatt gcttttcttt tttcacatca
    26521 cctgtgacag gctttgtgtg ttttgtgggg tgcttaaacg cccggctcct tatgcagtcg
    26581 acgacatgca gtgccatcct cacggaacag atggcaacgc tctggcggca ggccgatagc
    26641 gaatgtggca ccttcttcta ccaacaccac gtcgttctgg cggtacacca ggttttgacg
    26701 aatggaaggg atctggatat agatttgggt ttcgttgccg agttgctcga cgacctgaac
    26761 ttcaccctca aggatgacgt cagcgatatc actcggcagt agatgttccg ggcgaatacc
    26821 cagcgacata ttggctccaa cctggacatc acggctttca acaggcagcc agacttgttg
    26881 acgatttggc atcggcagct ccacctgcac ttgatcgatt gcggtggcgg tcacttttac
    26941 cggcaggaag ttcatctttg gcgaaccgat aaatccggcg acaaaacggt ctgccggata
    27001 gtggtacagt tccagcggtt tcccaacctg tgccacgcga ccggcgtcca gcaccacgat
    27061 tttgtcggcc agcgtcatcg cttcgacctg atcgtgggtg acgtaaatca ttgtgcggcc
    27121 caggcgttta tgcagacggg agatttcgat acgcatttgc acacgcagtg cagcatcgag
    27181 gttggagagc ggttcatcga gcaaaaatac gcttggctcg gccaccagcg tacggccaat
    27241 cgccacgcgc tgacgctgac caccggagag cgctttcggt ttgcgatcca gcaaatgcgc
    27301 cagttgcagc acttccgcca cctggttgac gcgttggtta atcacctctt tttttgcgcc
    27361 agccagtttc aggccaaatg acatgttttc tgctactgac aggtggggat agagcgcgta
    27421 agactgaaac accataccaa cgccgcgttc tgctggcgga gtgtcattca tccgtttctc
    27481 accgatgaac aggtcgccgc tggtgatcgt ctcaagcccg gcaatcatgc gcagtaaagt
    27541 cgatttaccg cagccagacg gtccgacaaa caccacgaat tcaccttcat ggatatcgag
    27601 attgatatct ttcgatacca cgacctcgcc ccaggctttc gttacatttt gcagctgtac
    27661 gctcgccatg cccttctccc tttgtaacaa cctgtcatcg acagcaacat tcatgatggg
    27721 ctgactatgc gtcatcagga gatggcttaa atcctccacc ccctggcttt tttatggggg
    27781 aggaggcggg aggatgagaa cgcggcttct gtgaactaaa ccgaggtcat gtaaggaatt
    27841 tcgtgatgtt gcttgcaaaa atcgtggcga ttttatgtgc gcatccccac attaccgcca
    27901 attctgtaac agagatcaca caaagcaacg gtggggcgta ggggcaagga ggatggaaag
    27961 aggttgccgt ataaagaaac tagagtccgt ttaggtgttt tcacgagcac ttcaccaaca
    28021 aggaccatag attatgaaaa taaaaacagg tgcacgcatc ctcgcattat ccgcattaac
    28081 gacgatgatg ttttccgcct cggctctcgc caaaatcgaa gaaggtaaac tggtaatctg
    28141 gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga
    28201 taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tccctcaggt
    28261 tgcggcaact ggcgatggcc ctgacattat cttctgggcg cacgaccgct ttggtggcta
    28321 cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta
    28381 tccgtttaca tgggatgccg tacgttacaa cggcaagctg attgcttacc caatcgctgt
    28441 tgaagcgtta tcgctgattt ataacaaaga cctgctgccg aacccgccaa aaacctggga
    28501 agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa
    28561 cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa
    28621 gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg gcgcgaaagc
    28681 gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag acaccgatta
    28741 ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg
    28801 ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt
    28861 caagggtcaa ccatctaaac cgttcgttgg cgtgctgagc accggtatta acgccgccag
    28921 tccgaacaaa gagctggcga aagagttcct cgaaaactat ctgctgactg atgaaggtct
    28981 ggaagcggtt aataaagaca aaccgctggg tgccgtagcg ctgaagtctt acgaggaaga
    29041 gttggcgaaa gatccacgta ttgccgccac catggaaaac gcccagaaag gtgaaatcat
    29101 gccgaacatc ccgcagatgt ccgctttctg gtatgccgta cgtactgcgg tgatcaacgc
    29161 cgccagcggt cgtcagactg tcgatgaagc cctgaaagac gcgcagactc gtatcaccaa
    29221 gtaatgctgt gaaatgccgg atgcggcgtg aacgccttgt ccggcctaca aaactgaaac
    29281 gcatgtaggc ctgataagac gcatcagtgt cgcatcaggc agttgttgtc ggataaggcg
    29341 tgaaagcctt atccgtcctg gaatgaggaa gaaccccatg gatgtcatta aaaagaaaca
    29401 ttggtggcaa agcgacgcgc tgaaatggtc agtgctaggt ctgctcggcc tgctggtggg
    29461 ttaccttgtt gttttaatgt acgcacaagg ggaatacctg ttcgccatta ccacgctgat
    29521 attgagttca gcggggctgt atattttcgc caatcgtaaa gcctacgcct ggcgctatgt
    29581 ttacccggga atggctggaa tgggattatt cgtcctcttc cctctggtct gcaccatcgc
    29641 cattgccttc accaactaca gcagcactaa ccagctgact tttgaacgtg cgcaggaagt
    29701 gttgttagat cgctcctggc aagcaggcaa aacctataac tttggtcttt acccggcggg
    29761 cgatgagtgg caactggcgc tcagcgacgg cgaaaccggc aaaaattacc tctccgacgc
    29821 gtttaaattt ggcggcgagc aaaaactgca actgaaagaa acgaccgccc agcccgaagg
    29881 cgaacgcgcg aatctgcgcg tgattaccca gaatcgtcag gcgctgagtg acattaccgc
    29941 cattctgccg gatggcaaca aagtgatgat gagctccctg cgccagtttt ctggcacgca
    30001 gccgctctac acactcgacg gtgacggcac gttgacgaat aatcagagtg gcgtgaaata
    30061 tcgtccgaat aaccaaattg gcttttacca gtccattacc gccgacggca actgggggga
    30121 tgaaaagcta agccccggtt acaccgtgac caccggctgg aaaaacttta cccgcgtctt
    30181 taccgacgaa ggcattcaga aaccgttcct cgccattttc gtctggaccg tggtgttctc
    30241 gctgatcact gtctttttaa cggtggcggt cggcatggtt ctggcatgtc tggtgcagtg
    30301 ggaagcgttg cgcggcaaag cggtctatcg cgtcctgctg attctgccct acgcggtgcc
    30361 atcgttcatt tcaatcttga ttttcaaagg attgtttaac cagagcttcg gtgaaatcaa
    30421 catgatgttg agcgcgctgt ttggcgtgaa gcccgcctgg ttcagcgatc cgaccaccgc
    30481 tcgcacgatg ctgattatcg tcaatacctg gctgggttat ccgtacatga tgatcctctg
    30541 catgggcttg ctgaaagcga ttccggacga tttgtatgaa gcctcagcaa tggatggcgc
    30601 aggtccgttc cagaacttct ttaagattac gctgccgctg ctgattaaac cgctgacgcc
    30661 gctgatgatc gccagcttcg cctttaactt taacaacttc gtgctgattc agctgttaac
    30721 caacggcggc ccggatcgtc ttggcacgac cacgccagcc ggttataccg acctgcttgt
    30781 taactacacc taccgtatcg cttttgaagg cggcgggggt caggacttcg gtctggcggc
    30841 agcaattgcc acgctgatct tcctgctggt gggtgcgctg gcgatagtga acctgaaagc
    30901 cacgcgaatg aagtttgatt aagggagata acaaaaatgg caatggtcca accgaaatcg
    30961 caaaaagcac gtttgtttat tactcacctg ctactgctac tttttatcgc ggcgattatg
    31021 ttcccgctgc tgatggtcgt cgctatctcg ctgcgtcagg gaaacttcgc gaccggtagc
    31081 ctgatcccgg agcaaatctc ctgggatcac tggaaactgg cgttaggttt tagcgttgaa
    31141 caggctgatg gtcgcattac gccaccgcca ttcccggtac tgctgtggct gtggaactcg
    31201 gtaaaggtcg ctgggatttc cgcgattggc attgtggcgc tctccaccac ctgcgcctac
    31261 gctttcgccc gtatgcgctt tccaggcaaa gcgacgctgc tgaaaggaat gctgattttc
    31321 cagatgttcc cggcagtact ttcactggtc gcgttgtatg cgttgtttga tcgtctgggt
    31381 gagtacattc cattcattgg cctgaatact cacggcggcg taattttcgc gtatctgggt
    31441 gggattgcgc tgcatgtctg gaccatcaaa ggctatttcg aaaccatcga cagttcgctg
    31501 gaagaagcgg ctgcgctgga tggtgcgaca ccgtggcagg ccttccgcct tgtcctgttg
    31561 ccgctgtcag taccgattct ggcggtggta ttcatcctgt cgtttatcgc tgccattact
    31621 gaagttccgg tcgcgtcgct gttactgcgt gacgtaaaca gctacaccct ggccgtgggg
    31681 atgcagcaat acctcaaccc gcaaaactac ctgtggggtg actttgccgc cgctgccgtg
    31741 atgtctgcat taccgatctc catcgtcttc ttgctggctc aacgctggct ggtcaacggc
    31801 ctgacggcag gtggtgtgaa aggttaaaga tgttgttctg ccaatgttat gccgctgcac
    31861 cctcaactta cgttatccca acttgtgact attattcggc gctccacgga gcgccttttt
    31921 ttctttcgtc tgcaatctga atcgttcgcc ggttaatatt cccatcatag aacttattat
    31981 ttttacgtta tttgttttcc cacttacgat aattctcttt cgtgctctga gtcacggcaa
    32041 tagtattgtt tcatcaattt ttgataatta tcacaattaa gatcacagaa aagacattac
    32101 gtaaacgcat tgtaaaaaat gataattgcc ttaactgcct gacaattcca acatcaatgc
    32161 actgataaaa gatcagaatg gtctaaggca ggtctgaatg aatacccagt ataattccag
    32221 ttatatattt tcgattacct tagtcgctac attaggtggt ttattatttg gctacgacac
    32281 cgccgttatt tccggtactg ttgagtcact caataccgtc tttgttgctc cacaaaactt
    32341 aagtgaatcc gctgccaact ccctgttagg attttgcgta gccagcgctc tgattggttg
    32401 catcatcggc ggtgccctcg gtggttattg cagtaaccgc ttcggccgtc gtgattcact
    32461 taagatttct gctgtcctgt tttttatttc tggtgtaggt tctgcctggc cagaacttgg
    32521 ttttacctct ataaacccgg acaacacagt acctatttat ctggcaggtt atgtcccgga
    32581 atttgttatt tatcgtatta ttggcggtat tggcgttggt ttagcctcaa tgctttcacc
    32641 aatgtatatt gcggaactgg ctccagctca tattcgcgga aaactggttt catttaacca
    32701 gtttgcgatt attttcgggc aacttttagt ttactgcgta aactatttta ttgcccgttc
    32761 cggtgatgcc agctggctga atactgacgg caggcgttat atgtttgcct cggaatgtat
    32821 ccctgcactg ctgttcttaa tgctgctgta taccgtgcca gaaagtcctc gctggctgat
    32881 gtcacgcggc aagcaagaac agacggaaag tatcctgcgc aaaattatgg gcaacacgct
    32941 tgcaactcag gcagtacagg aaattaaaca ctccctggat catggccgca aaaccggtgg
    33001 tcgtctgctg atgtttggcg tgggcgtgat tgtaatcggc gttatgctct ccatcttcca
    33061 gcaatttgtc ggcatcaatg tggtgctgta ctacgcgccg gaagtgttca aaacgctggg
    33121 ggccagcacg gatatcgcgc tgttgcagac cattattgtc ggagttatca acctcacctt
    33181 taccgtactg gcaattatga ctgtggataa atttggtcgt aagccactgc aaattatcgg
    33241 cgcactcgga atggcaatcg gtatgtttag cctcggtacc gcgttttaca ctcaggcatc
    33301 gggtattgtg gcgctactgt cgatgctgtt ctatgttgcc gcctttgcca tgtcctgggg
    33361 tccggtatgc tgggtactgc tgtcggaaat cttcccgaat gctattcgtg gtaaagcgct
    33421 ggcaatcgcg gtggcggccc agtggctggc gaactacttc gtctcctgga ccttcccgat
    33481 gatggacaaa aactcctggc tggtggccca tttccacaac ggtttctcct actggattta
    33541 cggttgtatg ggcgttctgg cagcactgtt tatgtggaaa tttgtcccgg aaaccaaagg
    33601 taaaaccctt gaggagctgg aagcgctctg ggaaccggaa acgaagaaaa cacaacaaac
    33661 tgctacgctg taatcttcct gtccagcacg ccgcgccatt tcggcgtgct gtctttttac
    33721 tcccgcttca gccgtttcga attacacagc cacagggtga tcaccagtaa caggatcgca
    33781 gccgagtaga tcaacacatc cagtggcgat ttatgatcga cgatgatcaa gcgcacaatg
    33841 gcggtgatcc caatatagac aaagtaacgt agcgggaagt gaaaaccgga ctgaaagtac
    33901 ttcacaatca gcgcgataaa ttcgaaatag agaaagtaaa ccaccagtcc ttctaccagc
    33961 tcatatttgc tggtttgttc tggcgcgaac agcacatcag ccagatgcac cgtttctttg
    34021 ccgaggaaga caaccaaaat caggccgagg cacagcaggc caagattgag tacggtctgc
    34081 aaaatggtgg agataaactc cacgcgcgga cgagagagtg acgtcatagc agtacctcct
    34141 tcacagtggc gagcttcctg tataacgcaa aaatgtgacg gagatctata ttttttgtgt
    34201 atgttttgtt caacctggat ttggagcgcg cctccaccaa agccggatat acctacccgt
    34261 aggccggata agccgcgcct ggcggcgcat ccggcaagac aaaccgcacc aaagccggta
    34321 attcaaaaat cacccattca ggtgattccc agccaccagc aatgcgcgaa aaatcgacaa
    34381 tgttcctctg gaacaaatga aacgtaacct gataaaggtg gtaaaaatga agccttattt
    34441 tgctgctttg atgttatcag tctctgttct tcctgcttat gctggtccgc tcggtactgc
    34501 tgataaagcc gacctaccgc aaagcaatgt ctccagccca atgatggccc agtcgctcag
    34561 gcaaccagac ctgcaaccga tatcgacgga caggaagaca gaatgttttc gcctttacac
    34621 accagatcgt aaaccggggg tgaactgcgt tcctgatggc tcaacaggtc attaagaatg
    34681 agggtgccag tgagtgcgaa aactggcacc tttcccatga cttagcggaa atttacgcta
    34741 cggtcgctgg tcatgtcata caactggaac ttacgcccaa gttgctgacc accgtcacgc
    34801 gtcagcggcg tccagccaat cgccgcacgg ctgcgtgtcg ggccagacga gaagagatcc
    34861 aacggtaccg agacatacac gcctttggtg aagtcccctt cgccgtactc ctctttcgaa
    34921 acgttggtga tcgtggcata gccacccacc accacgccgc tgtcaaagcg tttagcgatc
    34981 tccagcgtgc cgcctttatc ccctgccaga tactgcccga cgctggcttt caccaacacg
    35041 tcctgggcga aagatggcgt ccagtaggcg gtcagatgtc cggttttcac gctgtagtcg
    35101 gtgaatttca tcatatcttt tgcactacgc cagtcgcgct gtttaacgta gttggcatcc
    35161 agaccaaacg cccagttact gtccagcgga cgatacaaca cttcggcccc cgcaccgcca
    35221 aacatggttt cgagataacc tccatagacc tgaccgtaga agccgttgcc cagatgctgg
    35281 aagtagttgg cttgcaggtt attcacatag acatcgttct gcacatactc gcgcacatgg
    35341 gtacgaacgc gcggtaagtg cgagtcctgc ggtggattgg tgtagttaaa cttgtcgtag
    35401 ttgttggcaa gatttgcaaa caggctgccg gtggtcagta gatggtccgt cagccacaaa
    35461 tctgccgttc ccatcacgcc cagctgatac atgtaaaagt tttccgggcc accgaccgac
    35521 tggttcagca ccggatcgat atggaaatca aagcgtgatt tgtcgatata ccagccctgc
    35581 tcggtggact tcggaaccac tggctcgacg cgtttgtgcg ccagcgtcgt ttcgtggccc
    35641 aacggctctc cggcgagatt atttttcagg ctggcgacat cggtttccgt cgtcacttgc
    35701 ggcatgttaa ggcgattttc cgtaatgcgg atcgttttga tcccatccgg cagatcgttc
    35761 atcacgatcc gattagcacg gatgatccct tcgcgcgaat cacgatattt cacctgctcg
    35821 ccggtaacat acagcgtatc gcctttcgcc tggatctgcg gatcggcaag tccggcgttg
    35881 tatttcagca acgttaactg attcgccacc accgaatgct gcaaaatggc atcctgcggc
    35941 tgcggttgat attgcgggcg ggcgttatcg atgtaggacg ggcgcagatc gttaaagttg
    36001 gtgcgcagcg tgacgccaaa cataaaggtg ttgccacgtt catagctaag gttaacgtcg
    36061 gcccaatcgg taacgcgata aatcgccccg acgttaaact tacttttttg ctccagcttc
    36121 ccggcaaaat cctgctgata attattacct tcatactcca gtttcagacg cagcggttgc
    36181 cagggcgtct ggtattccac gccgccaaac agtgaggcag gaccgtggaa catctggcta
    36241 ccgtcgatag atcccgcctg tttgtagctg ttatcgcgat agcaatattt atcactggct
    36301 gaacagagcg gattttttac attaccgctg gtgcccagat atccccagcc caggccgagc
    36361 gtaaaatcga acggccccca ggctttgctg gcaacaagat attccgcatc aaacagcccc
    36421 gtaccgccga tatcccgcgc accaaccgcc acttgcggca gccagtaact ctcttcccac
    36481 aaacgcagtt tgagatcgaa ggctttatct ttatacgttt gatcgccaga gaacgcttcg
    36541 acgctgctgt actgccgggt gcgcacgtcg gtgtagcgca gcgttgtttc cagccacggg
    36601 aagagttgca ctgaagctga gtaataacgg tactgatcgt tatcgcgata gttcagactc
    36661 aactcccctt cccgtgccat acgcgcggtg ggagtttgta ataatcctac gccaccgaaa
    36721 tccgactgcg acggaccaat gggcgcagga tatgtttcgc cgtagcaagc tgtgctaatg
    36781 cccagcgcca gtaagctaag cagatgtctt tttttcatta ttggggtatc cgctgcgtca
    36841 gagtctgaag aatgtcggcg ttaagggcat caggcgtctc actccagacg gaatctgcga
    36901 ggccaacata aataatgctg ccgggcatcg gctctacgtg acgcttgttc cagtaagcca
    36961 ccggcgcttt ttgcgtgcgt ccgtccgggt aaacgaccca cgcgtagctg cgatccgcac
    37021 cgctgagcag gctctggtca gagagatagc tcgccacgtc gcgacctgga gtgaatggct
    37081 gattgccagg atggctgata agcccgaaca acgtgaccgt ggacggcggt ggtccgaccc
    37141 acagcgtgta gttgccctgc aacggcgggt taccgcgttc ggcaacgcgg acgatatcgg
    37201 gatcaagatt gattttttgc ctgcctgtca ctttcaatgc ctggatttgc tggcgtaagg
    37261 cgttaatggc ggcggcatcg tcggtgctgg aatctgcgcc ctgttctgcc agccgtgtca
    37321 gcaacgcctg ctgctgacgc aatgctgccg ccgttgccag ctcttcgcta atcaccgcac
    37381 caggccacca gctgtttgcc agtcgaggtt gccctaccag atcgattaaa tgctctgcgc
    37441 ccgttagcgt tttggcctca ctgctgccat tgctgaacac cttgacggtt cctgccgcga
    37501 agaccgatga cgctcccaca ctcaaaagca acgcgacaat agtttgttta atcattgttt
    37561 tgccgccttg atgagagtgg ttttcaccgg gaaataatcc gcgccgagat actgttccga
    37621 ctggcgaatt tgcccttcgc tgtcgatcca gtagcggtta tgccagcgcg cctggtcggt
    37681 ggagacttct tcgtccagca cgcgaaccag ggtttcatcg ctgccgacct tgacggtatc
    37741 ggtgccatcc catttgaaga ctgagcgtgc ggtggcgtag cgtacctgct ggtactcggt
    37801 ccagcccatc gtgcgcgtcc atgttgcacc atcaacaatt tgtgcgggtt taatcagcgg
    37861 gtcggcggca aggttattca cttcaatcag gttgtcgccg ccaagcaaag tcttcaccag
    37921 acgaccatgt tgtgtgacaa gagtggcttg atcctgagtg acccattttt gttgtccgtc
    37981 ttcagcgaag gccagcacca caaacaattg cggcccgcca ttaagctgca tgtactggct
    38041 ggcgtagggc atattttgaa tatcatcgtc cgtcagctgt acgcctggcg tgccgaacag
    38101 actgtcccac agtgaattgc ccagctcttt agtcgtggct gaacaggcct gtaatagcag
    38161 gcagataaga atgagtgcag gtcgcttcac gactcttctc cagaggggca gaataaccac
    38221 accggagtgt ggttatggta aatcccttca tacttcaagc cacagatgca atgactcaaa
    38281 ttattttggg gatacatact ttattactgg gtactggtgg tcgtggttgt ggtggtcccg
    38341 gtattagaac catcaccgcc accggttgcc gcgagcgcga cacccaccgc cgagcttacg
    38401 gtgctgacgc tggtcgcgga ggaactcccc gccgaaaccg acgttgctgc cgactctgcc
    38461 gcttcgccca cctgtacagg tgcagcccac gcagaagtcg ccgcaagcgc agatatggca
    38521 aaaatgccat acagaacttt tttcataaca atttcccttc attgaatgaa tggagattta
    38581 cccaaaaaca tttcgggcgg gatcgagtat acacaactaa agcatgcgaa ttacggcgcg
    38641 gggaattagc gatggagttt taggataatt ggaatgaata tcaaatataa gtttcaactt
    38701 atatttaaat aacaataaaa atcataagct taaaataaaa aacaaaagta attaatctgc
    38761 gtattctccg aaataaacaa cgattaaaaa tctattttag gattaacctc acggtatgat
    38821 ttccgttaaa tcacagacaa ggggatttat ctgataaaac aatgccgact tattgtcggc
    38881 attgttttta aggttattta actcctcata ctatgcatta atgtcgatat tggcatcagg
    38941 ctccggttgc cggatgcggc gtaaacgcct tatccggcct acatatcggc atcaggcacg
    39001 catgatgatt aaccgcgcca cgctttatag cggttaatca gaccattggt cgagctatcg
    39061 tggctgctga tttctttatc atctttcagc tctggcagaa tacggtttgc cagttgttta
    39121 cccagctcca cgccccactg gtcgaaggtg aagatgttca ggatcacgcc ctgagtaaag
    39181 attttgtgct catacagcgc aatcaacgca cccaggctga acggagtgat ttcgcgcagc
    39241 aggatggagt tggtcgggcg gttaccttcg aacactttga acggcaccac gtagtcaagc
    39301 gttgccggat ctttaccctg atcgcgatat tcctgctcaa ccacttcgcg ggatttacca
    39361 aacgccagcg cttcggtctg ggcgaagaag ttagacagca gtttctggtg gtgatcagag
    39421 agcgggttat gggtgatagc cggggcgatg aaatcgcacg gtaccatttt ggttccctgg
    39481 tggatcagct ggtagaacgc gtgctgaccg ttagtgcctg gttcacccca gataatcggg
    39541 ccagtctggt aatccacaac gttaccgtta cggtcaacat acttaccgtt ggactccata
    39601 ttgccctgct ggaagtacgc cgcgaaacgg tgcatatact ggtcatacgg cagaatcgct
    39661 tcagtttccg caccaaagaa attgttgtac cagatgccaa tcagcgccag caggacaggc
    39721 aggtttttct cggcaggcgt ggtggagaaa tgcttgtcca ttgcgtgtgc gccagaaagc
    39781 agttcaacga agttatcaaa gccgatggag agaacaatcg acaggccaat cgctgaccac
    39841 aaagagtaac ggccgccgac ccagtcccag aactcgaaca tgttggcagt atcaatacca
    39901 aactcgccaa cggctttggc attggttgag agcgccgcaa aatgtttcgc aacgtgtttt
    39961 tcatcacctg ccgctttcag gaaccagtca cgcgcgctat gggcgttggt catggtttcc
    40021 tgagtggtga aggttttaga tgctaccagg aacagcgtgg tttccgggtt tacttttttc
    40081 agcacttccg agatgtgagt cccatcgacg ttagaaacaa agtgcatgtt caggtggttt
    40141 ttgtacggac gcagtgcttc ggtcaccatg tacgggccga ggtcagaacc gccgatcccg
    40201 atgttcacta cgtcagtgat tgctttgccg gtataaccct tccactcacc ggagataatc
    40261 gcttccgaga aggttttcat cttctccagc accgcgttga cttctggcat aacatctttg
    40321 ccatcaacca gaatcggggt attgctacga ttacgcagcg ctacgtgcag cacggcgcgg
    40381 ttttcagtgc ggttgatctt ctcgccagag aacatcgact taatcgcgcc cgccagatcg
    40441 cactctttcg ccagatcctg taatttcgcc agcgtctctt cagtgatgcg gtttttggag
    40501 taatccacca gcatctgatc gtcgaaggtt gcggagaact tagaaaaacg atcgccgtct
    40561 ttagcaaaaa gatcggcgat cgtaacgtct ttcatttcat cgaagtgttt ctgtagtgcc
    40621 tgccaggcag cggtctgcgt tggattgatg tttttcatta gcaatactct tctgattttg
    40681 agaattgtga ctttggaaga ttgtagcgcc agtcacagaa aaatgtgatg gttttagtgc
    40741 cgttagtgta atgttgagtg taaaccctta gcgcagtgaa gcatttatta gctgaactac
    40801 tgaccgccag gagtggatga aaaatccgca tgaccccgtc gttgacaacc gccccgctca
    40861 ccctttattt ataaatgtac tacctgcgct agcgcaggcc agaagaggcg cgttgcccaa
    40921 gtaacggtgt tggaggagcc agtcctgtga taacacctga gggggtgcat cgccgaggtg
    40981 attgaacggc tggccacgtt catcatcggc tacaggggct gaatcccctg ggttgtcacc
    41041 agaagcgttc gcagtcgggc gtttcgcaag tggtggagca cttctgggtg aaaatagtag
    41101 cgaagtatcg ctctgcgccc acccgtcttc cgctcttccc ttgtgccaag gctgaaaatg
    41161 gatcccctga cacgaggtag ttatgtctga aattgttgtc tccaaatttg gcggtaccag
    41221 cgtagctgat tttgacgcca tgaaccgcag cgctgatatt gtgctttctg atgccaacgt
    41281 gcgtttagta gtcctctcgg cttctgctgg tatcactaat ctgctggtcg ctttagcgga
    41341 agggctggaa cctggcgagc gattcgaaaa actcgacgct attcgcaaca tccagtttgc
    41401 cattctggaa cgtctgcgtt acccgaacgt tatccgtgaa gagattgaac gtctgctgga
    41461 gaacattact gttctggcag aagcggcggc gctggcaacg tctccggcgc tgacagatga
    41521 gctggtcagc cacggcgagc tgatgtcgac cctgctgttt gtcgaaatcc tgcgcgaacg
    41581 cgatgttcag gcacagtggt ttgatgtacg taaagtgatg cgtaccaacg accgatttgg
    41641 tcgtgcagag ccagatgtag ccgcgctggc ggaactggcc gcgctgcagc tgctcccacg
    41701 cctcaatgaa ggcttagtga tcacccaggg atttatcggt agcgaaaata aaggtcgtac
    41761 aacgacgctt ggccgtggag gcagcgatta tacggcagcc ttgctggcgg aggctttaca
    41821 cgcatctcgt gttgatatct ggaccgacgt cccgggcatc tacaccaccg atccacgcgt
    41881 agtttccgca gcaaaacgca ttgatgaaat cgcgtttgcc gaagcggcag agatggcaac
    41941 ttttggtgca aaagtactgc atccggcaac gttgctaccc gcagtacgca gcgatatccc
    42001 ggtctttgtc ggctccagca aagacccacg cgcaggtggt acgctggtgt gcaataaaac
    42061 tgaaaatccg ccgctgttcc gcgcgctggc gcttcgtcgc aatcagactc tgctcacttt
    42121 gcacagcctg aatatgctgc attctcgcgg cttcctcgcg gaagttttcg gcatcctcgc
    42181 gcggcataat atttcggtag acttaatcac cacgtcagaa gtgagcgtgg cattaaccct
    42241 tgataccacc ggttcaacct ccactggcga tacgttgctg acgcaatctc tgctgatgga
    42301 gctttccgca ctgtgccggg tggaggtgga agaaggtctg gcgctggtcg cgttgattgg
    42361 caatgacctg tcaa
//
LOCUS       DAJTUP010000025        41947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTUP010000025 DAJTUP010000000
VERSION     DAJTUP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.025"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..322
                     /locus_tag="OO468_001378"
     CDS             <1..322
                     /locus_tag="OO468_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6258549.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OO468_001379"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OO468_001379"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR6258550.1"
                     /translation="MKPTTIASLQIYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OO468_001380"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OO468_001380"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR6258551.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO468_001381"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO468_001381"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR6258552.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OO468_001382"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OO468_001382"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR6258553.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OO468_001383"
     CDS             complement(3726..4955)
                     /locus_tag="OO468_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6258554.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OO468_001384"
     CDS             complement(5019..5459)
                     /locus_tag="OO468_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6258555.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OO468_001385"
     CDS             complement(5564..6334)
                     /locus_tag="OO468_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6258556.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OO468_001386"
     CDS             complement(6331..7257)
                     /locus_tag="OO468_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6258557.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OO468_001387"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OO468_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR6258558.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO468_001388"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO468_001388"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR6258559.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OO468_001389"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OO468_001389"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR6258560.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO468_001390"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO468_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR6258561.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMVIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OO468_001391"
     CDS             12964..13311
                     /locus_tag="OO468_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR6258562.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OO468_001392"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OO468_001392"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR6258563.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OO468_001393"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OO468_001393"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR6258564.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OO468_001394"
     CDS             complement(15131..15493)
                     /locus_tag="OO468_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR6258565.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO468_001395"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO468_001395"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR6258566.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OO468_001396"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OO468_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO468_001397"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO468_001397"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR6258567.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO468_001398"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO468_001398"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR6258568.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO468_001399"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO468_001399"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR6258569.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO468_001400"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO468_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR6258570.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OO468_001401"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OO468_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR6258571.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO468_001402"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO468_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR6258572.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO468_001403"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO468_001403"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR6258573.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OO468_001404"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OO468_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR6258574.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OO468_001405"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OO468_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR6258575.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OO468_001406"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OO468_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OO468_001407"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OO468_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR6258576.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO468_001408"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO468_001408"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR6258577.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OO468_001409"
     CDS             complement(36348..36386)
                     /locus_tag="OO468_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258578.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OO468_001410"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OO468_001410"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR6258579.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OO468_001411"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OO468_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR6258580.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OO468_001412"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OO468_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR6258581.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO468_001413"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO468_001413"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR6258582.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO468_001414"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO468_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR6258583.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO468_001415"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO468_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR6258584.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
BASE COUNT        10082 a        11330 c        10402 g        10133 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagatatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 caccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgtctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagc
//
LOCUS       DAJTUP010000026        40069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTUP010000026 DAJTUP010000000
VERSION     DAJTUP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.026"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..728)
                     /locus_tag="OO468_001416"
     CDS             complement(<1..728)
                     /locus_tag="OO468_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR6258585.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAG"
     gene            974..1600
                     /gene="gstB"
                     /locus_tag="OO468_001417"
     CDS             974..1600
                     /gene="gstB"
                     /locus_tag="OO468_001417"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR6258586.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1597..2711)
                     /gene="yliI"
                     /locus_tag="OO468_001418"
                     /pseudo
     CDS             complement(1597..2711)
                     /gene="yliI"
                     /locus_tag="OO468_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2822..3205)
                     /gene="bssR"
                     /locus_tag="OO468_001419"
     CDS             complement(2822..3205)
                     /gene="bssR"
                     /locus_tag="OO468_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR6258587.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3418..4743
                     /gene="rimO"
                     /locus_tag="OO468_001420"
     CDS             3418..4743
                     /gene="rimO"
                     /locus_tag="OO468_001420"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR6258588.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4790..6118)
                     /locus_tag="OO468_001421"
     CDS             complement(4790..6118)
                     /locus_tag="OO468_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR6258589.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6126..8468)
                     /locus_tag="OO468_001422"
                     /pseudo
     CDS             complement(6126..8468)
                     /locus_tag="OO468_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8646..9557)
                     /gene="gsiD"
                     /locus_tag="OO468_001423"
     CDS             complement(8646..9557)
                     /gene="gsiD"
                     /locus_tag="OO468_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR6258590.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9560..10480)
                     /gene="gsiC"
                     /locus_tag="OO468_001424"
     CDS             complement(9560..10480)
                     /gene="gsiC"
                     /locus_tag="OO468_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR6258591.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10498..12036)
                     /gene="gsiB"
                     /locus_tag="OO468_001425"
     CDS             complement(10498..12036)
                     /gene="gsiB"
                     /locus_tag="OO468_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR6258592.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12056..13927)
                     /gene="gsiA"
                     /locus_tag="OO468_001426"
     CDS             complement(12056..13927)
                     /gene="gsiA"
                     /locus_tag="OO468_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR6258593.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13914..14879)
                     /gene="iaaA"
                     /locus_tag="OO468_001427"
     CDS             complement(13914..14879)
                     /gene="iaaA"
                     /locus_tag="OO468_001427"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6258594.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15083..16318
                     /gene="moeA"
                     /locus_tag="OO468_001428"
     CDS             15083..16318
                     /gene="moeA"
                     /locus_tag="OO468_001428"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR6258595.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16318..17067
                     /gene="moeB"
                     /locus_tag="OO468_001429"
     CDS             16318..17067
                     /gene="moeB"
                     /locus_tag="OO468_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR6258596.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17246..17908)
                     /gene="fsa"
                     /locus_tag="OO468_001430"
     CDS             complement(17246..17908)
                     /gene="fsa"
                     /locus_tag="OO468_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR6258597.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18039..18938
                     /locus_tag="OO468_001431"
     CDS             18039..18938
                     /locus_tag="OO468_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR6258598.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            18944..21376
                     /locus_tag="OO468_001432"
     CDS             18944..21376
                     /locus_tag="OO468_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR6258599.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21522..22337
                     /gene="ybiV"
                     /locus_tag="OO468_001433"
     CDS             21522..22337
                     /gene="ybiV"
                     /locus_tag="OO468_001433"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR6258600.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22489..23754
                     /locus_tag="OO468_001434"
     CDS             22489..23754
                     /locus_tag="OO468_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR6258601.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(23995..25587)
                     /locus_tag="OO468_001435"
     CDS             complement(23995..25587)
                     /locus_tag="OO468_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR6258602.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25806..26726
                     /gene="ldtB"
                     /locus_tag="OO468_001436"
     CDS             25806..26726
                     /gene="ldtB"
                     /locus_tag="OO468_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6258603.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26784..27902)
                     /locus_tag="OO468_001437"
     CDS             complement(26784..27902)
                     /locus_tag="OO468_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR6258604.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27899..28366)
                     /gene="mntR"
                     /locus_tag="OO468_001438"
     CDS             complement(27899..28366)
                     /gene="mntR"
                     /locus_tag="OO468_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR6258605.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28552..28680
                     /gene="mntS"
                     /locus_tag="OO468_001439"
     CDS             28552..28680
                     /gene="mntS"
                     /locus_tag="OO468_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR6258606.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            28952..30535
                     /gene="opgE"
                     /locus_tag="OO468_001440"
     CDS             28952..30535
                     /gene="opgE"
                     /locus_tag="OO468_001440"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR6258607.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30583..31098)
                     /gene="ompX"
                     /locus_tag="OO468_001441"
     CDS             complement(30583..31098)
                     /gene="ompX"
                     /locus_tag="OO468_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR6258608.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31151..31216)
                     /locus_tag="OO468_001442"
     CDS             complement(31151..31216)
                     /locus_tag="OO468_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258609.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31451..32338
                     /gene="rhtA"
                     /locus_tag="OO468_001443"
     CDS             31451..32338
                     /gene="rhtA"
                     /locus_tag="OO468_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR6258610.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32637..33140
                     /gene="dps"
                     /locus_tag="OO468_001444"
                     /gene_synonym="pexB"
     CDS             32637..33140
                     /gene="dps"
                     /locus_tag="OO468_001444"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR6258611.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33544..34290
                     /gene="glnH"
                     /locus_tag="OO468_001445"
     CDS             33544..34290
                     /gene="glnH"
                     /locus_tag="OO468_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR6258612.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34429..35088
                     /gene="glnP"
                     /locus_tag="OO468_001446"
     CDS             34429..35088
                     /gene="glnP"
                     /locus_tag="OO468_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR6258613.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35085..35807
                     /gene="glnQ"
                     /locus_tag="OO468_001447"
     CDS             35085..35807
                     /gene="glnQ"
                     /locus_tag="OO468_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR6258614.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36068..38292
                     /gene="ybiO"
                     /locus_tag="OO468_001448"
                     /pseudo
     CDS             36068..38292
                     /gene="ybiO"
                     /locus_tag="OO468_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38289..39215)
                     /gene="rlmF"
                     /locus_tag="OO468_001449"
     CDS             complement(38289..39215)
                     /gene="rlmF"
                     /locus_tag="OO468_001449"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR6258615.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39491..39751
                     /locus_tag="OO468_001450"
     CDS             39491..39751
                     /locus_tag="OO468_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6258616.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
BASE COUNT         9971 a        10580 c         9754 g         9764 t
ORIGIN      
        1 cccgcacctg ccgtggttcc tgtcttcatg ccatcaacat tcagattgct gctccacagc
       61 agacggttac ggttaggctg acgaattttg ttgaaggtga actctttctc tttatgaatg
      121 gcgtactctt ccggcacatc gtggatcaat gctttaccca gcaatgccat atcgcgcgcg
      181 gtactgaact gccccggcgc atccaggccg tgcaccgtct ggaaggtagt gttggtcagc
      241 cccagttttt tggcataacc attcatcaga ccaataaagg actcctggct cccggcaacg
      301 taatctgcca gcgcaataca ggcgtcatta ccggactgga taatcacacc tttgttcaag
      361 tctgccaccg aaacctgatc gcccggtttg aggaacatta ccgatgaacc acgcagtgcc
      421 ggatttcccg tcgcccaggc atctttaccg accgtcacca tatcggtgag tttaatctta
      481 tcggccttaa gcgcctgccc aaccacatag ctggtcatga ttttagtcag gctcgcagga
      541 tccagtttct catccgcatt gccttctgcc agcactttac cgctggcgta atccattaaa
      601 atccatgcac gcgcatccac gctcggcgct tcaacggttt gttcggccgc gaatgccgtt
      661 ggggcaaaaa ggaataaaaa tgcagaaccc gctgcaagac cacgaaggag agaggagtat
      721 tgcgtcataa atgccacccg agtatccatt caaaaaaata cgttatatcg ccgtatgata
      781 agttcggtga ttaataacgc acacacaaac ttaaagaaac cgtaagttgg taaagttttt
      841 aaagtttatg caatatcctg ccgcttcgct gcttctggtg tcattttttt gccagctgtt
      901 gcttaaaagt taactttact tcaccatcgg agtcctgttc agtctgtggc tgcctgtcgg
      961 aaaaggggtg agtatgatta cgctgtgggg tcggaataat tcaactaacg taaaaaaagt
     1021 attgctgacg ctcgaagaac tggaactacc ttatgagcaa attctcgcgg gccgtgagtt
     1081 tgggataaat cacgatgctg attttctggc gatgaaccct aacgggctgg tgccgttgtt
     1141 gcgtgacgac gaaagtgatc tcattctttg ggaatcaaac gccattgtcc gctatctggc
     1201 ggcacagtac gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg cggaagcgga
     1261 aaaatggatg gactgggcaa accagacgct cagtaatgct catcgcggga tcctgatggg
     1321 attagtcaga acaccaccgg aagagcgcga tcaggccgcc attgatgcca gttgcaaaga
     1381 gtgcgacgcc ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat ggttctctgg
     1441 cgacgagttt ggtgtgggcg atatcgctat cgcaccgttc atctacaatt tgtttaacgt
     1501 tggcctgacc tggacaccgc gtccaaatct gcaacgctgg tatcagcaac tcactgaacg
     1561 ccccgcagtg cgcaaagtgg tgatgattcc cgttagctaa ttgcgtgggc taactttaag
     1621 taattcccca ctggactcgt cggtgagaac gtataaataa ccgtcgggtc cagtgcgaac
     1681 atcacgaatt cgctgccctc tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc
     1741 gttgacgctc atcacaatga catctttatc tttcagctcc caataaataa tttttgctgc
     1801 cactggggga atttatcgct gttatagaag gccatgccgc tcacagcggg cgaatctttc
     1861 cagtaaaaaa caggttgctc ggtcccggcg acgatctccc ctttcgcttc cggtatctta
     1921 aagcctgaat agttgattcc ccaggttgcc agcggccagc cgtagttttt gcctttttgc
     1981 gggatattaa tttcatcacc accgcgcggg ccatgttcat tcagccacag tgcattactc
     2041 cacggattca tcgccattcc ttgcggatta cgaatgccat aagaccagat ctcggcgcgc
     2101 gcaccggatt cctttataaa aggattatca tccgggattt cgccctggtc ggtcagacgc
     2161 accagtttgc cctgtaattt atccagatcc tgcgccgtcg ggcgctgatt gttttcgccc
     2221 agagcaataa aaagataacc tttaccgtcg aataccagcc gcccgccaaa atggttgccg
     2281 gtagacagtt ttggcatctg gcggaagacg gtgcggaagt cggtcacttt tgagagatca
     2341 tcacttaagc ggccataacc cacagcagtt ccggctttgc catcatcgcc aacttcggaa
     2401 taacttaacc agatgcggcg agactgagca aaatcaggcg ctaaaaccac gtccagcagg
     2461 ccgccctgcc cgtgcgccca aacgtccgga actccggaaa gcggcgcaga taatcctttt
     2521 cctgcttgcc agtgacgcaa ctcgccgccg cgcagagtga ttaacatacc gtgattatcg
     2581 ggtaaaaagg ccagtgccca gggatggtcg agtttgtctt gcagtacttc gacatttacc
     2641 gttgcaggag ccgcccagag agcggaagaa agacaaataa ggggcacaag gaaaaaggat
     2701 tgtcgatgca taacgcctcc ggattgactc tggcttaaag cgtagtcagt ggaggagata
     2761 acaaattcat ttttacaaaa acttaaacat gaagggggag acgctttctc ccccttagtt
     2821 ttcaggcctt ctcaagcatg gcgtgcttct gcaggctctg gatactcagc gttaagctca
     2881 tcagacaatt ttcaagctta tcggcgttga cggtaataac agtcgggcaa tcatggtgcc
     2941 cactcatcaa acatactgcg gctgtcgcta atgcttcttc agcatgatga agagcactcc
     3001 actcttcctg atccagatga agattcaacc gcagcgattt atcgtgcagt tcgcgattca
     3061 gtttaaaaaa gttatctcgt agatgattgc tttcgctgac ggacatgtat ccttttgcct
     3121 tcctcagttg cacgtatgca gcgaggtcaa cgcgtgcgtc gatcaaccgg ttcagcagat
     3181 cgattcgctg tctgtcaacg aacatacttc gatcctcctc tttccagcct tgttgcaatt
     3241 aaatagtatg gctatttttt gagcaaaatg ctgtccagga tcaattcttc acgccgaatt
     3301 aacaaaattt acatttcctt cgcctccgcc agcgaatgag attgcgatac aaattttatc
     3361 cagaactctg tagaatccct gccctgacct ttcctgtaac tgaatatttt ttaagctatg
     3421 agcaaagtaa ctccccagcc gaaaatcggc tttgtttccc ttggctgtcc gaaaaacctt
     3481 gtcgattcag agcgtattct caccgaactc cgcactgaag gttatgacgt ggtaccgagc
     3541 tatgacgatg ctaacatggt gatcgtcaac acctgcggct ttattgacag cgcggtacaa
     3601 gaatcactgg aagccattgg tgaagcgttg aatgaaaacg gcaaggtaat tgtgaccggt
     3661 tgtctggggg caaaagaaga tcagatccgc gaagtccacc cgaaagtgct ggaaatcacc
     3721 gggcctcata gctatgagca ggttctggag cacgttcatc actacgtgcc aaaaccgaaa
     3781 cacaacccat tcctgagcct ggtgccagaa caaggtgtga aactgacgcc gcgtcattat
     3841 gcctatctga aaatttcgga aggctgtaat caccgctgca ccttctgcat tattccgtct
     3901 atgcgcggcg acctgatgag ccgtccgatt ggcgaagtgt taagtgaagc gaaacgtctg
     3961 gtagatgcgg gcgttaaaga gattctggtg atctcgcagg atacttccgc ctatggcgtt
     4021 gatgttaaac atcgtactgg cttccacaac ggcgagccgg taaaaaccag catggtcagc
     4081 ctgtgcgaac agttatcgaa actggggatc tggacacgtc tgcactacgt ttacccttat
     4141 ccgcatgtgg acgacgtcat cccactgatg gcagaaggca aaatcctgcc gtatctggac
     4201 attccgttgc agcacgccag cccgcgcatt ctcaaactga tgaagcgtcc gggttctgta
     4261 gatcgccaac tggcgcgcat caaacagtgg cgcgaaatct gcccggaact gaccctacgc
     4321 tcaaccttta ttgtcggctt ccctggcgag acggaagaag atttccagat gctactcgac
     4381 ttcctgaaag aagcacgtct ggatcgcgtt ggctgcttta aatacagccc ggttgaaggt
     4441 gcagacgcca atgccctgcc tgaccaggtt ccggaagaag tgaaagaaga acgctggaac
     4501 cgtttcatgc agctgcagca gcagatttcc gccgagcgcc tgcaagagaa agtgggccgt
     4561 gaaattctgg tgattatcga cgaagtggac gaagaaggcg cgattggtcg cagcatggca
     4621 gatgcaccgg aaatcgacgg cgcggtttat ctcaatggtg aaaccaacgt taagccgggt
     4681 gatatcctgc gtgtgaaagt cgagcacgcc gatgagtacg atttgtgggg tagccgggtt
     4741 taattaattc aggccgggca aaatcatttg tccggcctgc gaataatgat tactgccagc
     4801 taatctgtgc cctgccgttt ttcttactct tatacagccg ctcatccgcc agcattttca
     4861 agcgttcgac ggtagtacat tcatttgaat gcgctacacc gatactggtg cttaaattaa
     4921 tgcttcccac ctggaaatga acgatttctt ttagtatcga tagcaactct tccagcgtgc
     4981 cgccagtata aatcaccgca aattcgtcac ctccgaagcg gtagacttta cctttatcta
     5041 ccgggatctg tgatttaagg attttcacca ctttcatcaa cacttcatcg cccagcaaat
     5101 gaccgaaggt gtcgttaatg tttttgaatt tatcgacgtc aaacagacac aaataaccgc
     5161 ctttttccgg cagcgctttt aaatcttcat caaaggattt tctgcccatt gctttggtca
     5221 ggtcgtccag gaacgccgag ttatacatac tgtagagttt tttgacatcg gcagccgaaa
     5281 agaaaagccc gccatccagt acgcgttcaa aatggttatt aatgatggct tctttctcca
     5341 caataccacg attaatcact ctgcgtacca gatagaggca actggcaccc aatatcactg
     5401 ccaccaatgc ggcaaagaaa ataatcaatg tacaaacccg caaataaaca tcttttttat
     5461 acgcaaatac gtacaatccg gtcggcaaat aggcgaagtg ctccatcgct tccgaaacgg
     5521 tgaagatggc gtttttagca ttatattcat caccggtcac cggtttaccg ttcttaaata
     5581 gagagaacat caggttctcg ctgtccatca ccagcgattt tgacagtaaa gaaaaacgct
     5641 ccagaccaag caaaaatatc agactacgat tgtgagttac cggatagagg taatagacga
     5701 aggttttacc gtcaaaagag ataaaccctg tatttttgac atgtcgatac ttcacaacct
     5761 gcataatatc tgcacaataa tttttctcga actcctttac ctgaatacat tcgtcactgg
     5821 aaatcagata aggaccgaca tcgtacaaat aagaggggga aaccgagtcg gcgtaagctg
     5881 tgcgcaaacc aaccaacagc tgttcctccg tgggatttag cgtgttgata agatcaattg
     5941 ccacgctacg ccgatattca gcaatatttg ccaccgcaaa agcagtatca ataacggact
     6001 gtttcaggct atacacccgt tcctttacca tttgagtgta gatcccgcag gcaactgctg
     6061 aaatcatgat aaaaatcagc agactgactg taagtacgaa cttattgatt ctggacatac
     6121 gtaaactact cttttactaa ttttccactt ttatcccaag cggagaatgg catgggtttc
     6181 tggtacagat acccctgagc ctgaacacat cctaatttaa ttaattcgcg cgcaacgtct
     6241 tcgctctcta cgccttccgc aatcaccggg atccggttat agcgtgccag atcgaaaata
     6301 atgcgcacaa tttttcccgc agtaccgaaa atatcgccgg taaattgcga gtccaccttt
     6361 atgctgtctg gattaatttc ataaaaacgt ttcaggtttg acaaaccggt gccaaagtca
     6421 tcaatcgata tccgtacccc ttgtttacgc agttgttcaa tattgagata aaaatgttca
     6481 ttaattcgct cgatcacggt ttcagtgatc tcaacacaga ggctgttcgc cttcaggcca
     6541 ttttctttca tgatggtggt aaaacgctca tgaaagtgcg gttcgtttaa ctgccagggc
     6601 gaaacattaa tatgcagtaa gaaatcatcc ttcagcgacg cacgttgact aaaatgtctg
     6661 aattaaccca ttgccgtttt aataatctga taacccagct cattgataaa cccgatatct
     6721 tcagcaatgg taatgaactt cagcggtgaa atgatccccc gctccgcaga aacccaacgg
     6781 cacaacgcct ccgcgctcag aatggcgcgg gtgttaatat caacgatcgg ttgatagacg
     6841 aggaatagtt cgccaatttc cattgcatgg cgaatattac gcgcaatcca gatattctct
     6901 tctatttcgt tgagtttcgc gtcatcacag aaaataaact cggagaacat atggtcacgg
     6961 atactggaaa tcgccagttt cagtttttct gcgtaactcc aggcgttttc cccctgcaaa
     7021 ggttcacaaa tggcagactt atggatgcgc agattgcggt agccaaagct gctgtcaaaa
     7081 tcgtttacca tcgctatttt acgggtaaat gtctgtacat tttcccgaca cagaagtaaa
     7141 tagagatcgg cattgaatgt cacgatatcg cagcattgcg catatttctc ggcaattcgc
     7201 tgacacaatt tcctgacgat tttattgcgt tcatcaacac caaacaccgc ctccacggct
     7261 tccagaccac acagtttgat gatccccaca gccagatact gattattgtt ggactcaata
     7321 atcgccatat ttttaataaa accgctttcc tgatcgtaag agatcagtga gagcaattta
     7381 tttgaccgtt caatttcggc atctttcatt tccgcgatcg aatttttgat gctgataatc
     7441 tctttcgcat cgccttcgcg aattttcaga gcctcgatct gatccgttga gtcgggcaaa
     7501 aacgccagcg tattaaccag ctcggtcatt tcagaaaatt gccgacggaa cacacgagtg
     7561 gtcatgaaat ataccaccag caacacgata ataatgaacg caaacggtag ataaaaaagt
     7621 ttatctgatg cgttcaataa attaccgcca ggataataaa aatagatttc gaacaatgca
     7681 ttatttggca ctgctgatgt ctttgcaata tactccgtat cgacagaatc aataatatta
     7741 taattacctt cgctattata aataatcagt ttttcacgag cgagttgatt cttggcaatg
     7801 atctcatgac cttgtttcaa aaatagaact tcatcattag cgctgatttg atcaagatac
     7861 tcgttcagat accgccagtt taaatcgaca tgaattttat ctaccgctaa caaatcgtga
     7921 tctttgccgt tgatacgtag cgtaaaagag atacattttt tacccggacg atcaaagcaa
     7981 ttgtatgccg gattccagct gttgttagag tcatctgaag ccaccgacca cggtcgtttc
     8041 gatgggttaa aatcggcaaa aataacccgc cctgactcgt cgacaagcgg tccatttact
     8101 tgttcctgat gataaacaac gattttatca ttcgccctcc gcgaaaagag ataatattta
     8161 ttatcatcaa gtaccagtcc caccgagtct aaaaatggca tcatttccat tttcagaatc
     8221 acggcgcgtt gaatctcctc tgtatttttt aataaataca gtgagtcctt taatgtcagg
     8281 gccgaacgtt caatatcttt aaagaaatag ccaacgtttt tctgaataag ttcaaagcgg
     8341 ctatcggcac gttttaccgc gcgttccgat accagttgat agttaatcat gaaaaaaaga
     8401 ccaataaatg acagtgctgc caataaaaat aaaattatca gccttatctt ttcgtataaa
     8461 ctcagcatgt taatacattt aacaaaataa ctatctgaac gctgtgccgc tgcaaaaata
     8521 gtcccttgct gcggcttttt aaccaaatta cccgattcta caaaatcagc agaaagttac
     8581 aacacttgct tagggcaata atttcctgtg aggtgattac cctttcaagc aatattcaaa
     8641 cgtaactatc ctttaatttt cggatccagc gcatcgcgta aaccatcgcc caacaaattg
     8701 aacgccagta cggtcagaaa aatagccagg gccggaaaaa cagcgacatg cggcgcgata
     8761 accatatccg ctcgtgcctc attgagcatt gctccccact ctggtgtcgg cggctgcgca
     8821 ccgaggccga gaaatgacag gctggcggca gagataatcg aggtgccaat gcgcatggtg
     8881 aaaaacacca cgatagaaga gacggtccca ggcaggatat gacgcaataa aatggtcata
     8941 tcgctggcac caatactgcg tgctgactca ataaaggttt gctgtttcaa caccagcgtg
     9001 ttgccgcgca ccaggcgggc aaacgcgggg atggaaaaaa tggcgactgc gataatcacg
     9061 ttagcaatgc cgcttcccaa caccgcaaca acagcgatcg ccagtaaaat acccgggaag
     9121 gcaaacagca catcgcaaat gcgcatgatc agccgatccc accagccttc ataatatcca
     9181 gccaacaagc ccagcaacgt ccctatcgcc acaccgataa acacggcaaa cacgcccgcc
     9241 gccagcgaga tttgcgcacc aaccaggaca cggctgaaaa tgtcgcgccc cagtgaatcg
     9301 acgccaaacc agtgctgcaa agaaggtccg ttattcagat tgtcataatc aaaataattt
     9361 tcggcgtcat agggagcgat ccagcgtgca aaaatggcca ccacaatcaa taaaataacg
     9421 aataatgcgg cggtcatcgc catatgctga cggcgaaatc gtcgccagaa ttcatgccac
     9481 ggtgtacgta cctggtcagg tttgaccagt ggcatggcgt ttaacaccgc ctgacgtcgc
     9541 cagttaaata gtcgcatcct tacttgtacc tgatagccgg gttaatggcg gcgtaaagca
     9601 catccaccac taagttgata agaataaatt ccagcgagaa aagcagaatt tccgcctgaa
     9661 tcaccgggta atcgcgcatt tctacagagt caacgagtaa gcgtccaagt ccgggccagt
     9721 tgaaaacttt ctccaccacg atagaaccac cgagcaaaaa gccaaactgt aagcccatca
     9781 tggtcactac cgggatcatc gcgttgcgta gcccgtgttt gaggacaacc caggtttcgc
     9841 tcaccccttt cgccctcgcg gtacgcatat aatcttcgct taaaacgtcg acaaatgacg
     9901 cacgggtaaa gcgcgccatc acggcggcca ctgccgcgcc gagcgtcagg gagggtaaaa
     9961 tgtagtgctg ccaactgtct gctcccacag taggcagcca gcccagttca acggagaata
    10021 cctgaattaa aaacatcccc agcgcaaatg ccggaaacga gatccccgac accgcaatgg
    10081 tcatactcaa tcgatccggc caacgattac gccagacggc ggcgataatt cccgccgcca
    10141 taccaaatat caccgcccag accatactgg ttatggtcag ccatagcgtt ggcataaagc
    10201 ggctggcaat ctcatcggca accggacgac gcgacaccat cgacaggcca aaatccccct
    10261 gcacagcatt gctgatatag tgccagaact ggtgatacag cggctgatcc aaccccagct
    10321 gctgacgcac cagttctata acctgcgcat cagcttcggg cccggcaatc aatcgcgccg
    10381 gatcgccggg cagcatgtgg acaaataaaa acaccagcac cgagacgata aacagcgtcg
    10441 gaatcaaccc cagtaatcgt ttgataacgt aataaagcat tccactccct gcgttgctta
    10501 ttgcaaatcc gcgtcttcaa agctgaagcc ggtatctggc atgatccaaa aaccggtcag
    10561 gtttttactg tgtgccgaca ccagtttttc taccaccagc gggatccacg gcgattcttg
    10621 ccagatgata tcctgcgccg ccttatataa gcgggtcttt tccgccggat cattcgtttt
    10681 cagtgcctga gccaggaagt catccacctg tttattgctg taaaacgcgg tattaaacag
    10741 cgtcggtggc cagttttgcg aggcaaacag cggcgatagt gcccagtcgg cttcgccggt
    10801 tgaagccgac cagccagtgt agaacatccg cacgccactc tctttttgcc ctttaccttc
    10861 aacttctgcc gcccgctgtc cggcatccat cgcggtcacc tgggctttaa tcccgacctg
    10921 cgctaactgc tgctgggtaa attgcagcac tttctgcgcg gtgctgtggt tatgtgacga
    10981 ccacagcgtg gtactgaaac cgttgggata tcccgcctct ttcagtaatt cgcgcgcttt
    11041 cactggatcg taaggccacg gtttataact ttgcgcgtag gcgatacttg gcggtaccac
    11101 accagtagct ggagttgcat agcccgcaaa ggcaactttc accagcgccg gacggttaat
    11161 ggcgtaattc agcgcctcac ggaccttcgg gttatcgaac ggcttttgcg tcacgttcat
    11221 actgatataa cgctgcataa ttgacggact ggccatcaac tcgatatttt tgtttttctc
    11281 cagcagtgtg gcctgctcgt agggaatggg gaaagcaaac tgcgcttcac cggtttgcag
    11341 cattgccgcg cgggtgttgt tatccgccac cggacgccag gttatgctgt ccagtttggg
    11401 caatcctggc tgccagtaac ccgcgaattt tttcaccttc acaaaatcgg tctgattcca
    11461 ggtatccagt tcatacggtc cggttcccac cggatgaaaa ccaatctcct tgccatattt
    11521 ttccagcgct gtcggtgaaa tcatcgcggt cgccggatgg gcaagaatat taataaacgc
    11581 tgagaacggc tgtttgaggg taatctttac cgttgtcgga tcgatcgcct ccgttttggc
    11641 aatattctta tacaggttat agcgtttaag atgattcgcc ggatcgctgg cccggtccag
    11701 attcgctttt accgccactg cgttgaaatc ggtgccatcc tggaatttaa ttccttcccg
    11761 caatttcacg gtgtaagtaa tgccgtcatc ggaaacggta taactctccg ccagcacgtt
    11821 tttcagtttc atctctttat ccagaccgaa cagcccctgg taaaacgatt tcgctacggc
    11881 ctgagataac gtgtcattcg cgtcatacgg atcgagcgtg gtgaaattcg atcctaccgc
    11941 caccaccaca tctttggcag cgaatgcaca agatgccatc aacgctgtcg caatgcccag
    12001 cgccactaac ccactacggt gtacagctct tgccatttta ttctccgcct gaatgttatc
    12061 tacgcatgaa tgcgtattct gattgtggtt gtgcgacgta atgccccggc ccgacgcatt
    12121 gcaacgagac ggctgccacc tcttcgccac gcagatgaat attgctggga agatcgtccg
    12181 acagcagtac acgctgcggt cgttgtcggg acggttcagc gaccggaact gccgccagta
    12241 atttacgcgt ataaggatgc tgcgggtttt cgaagaccgc gcaccgtgaa ccaatttcaa
    12301 caatttgccc gagatacatc accgccacac gatgactaat ccgctctacc acagccatat
    12361 cgtgggagat aaacagatac gcaatgccga aatcacgctg gagatcgagc aacaagttga
    12421 taatctgccc gcgaatagaa acatccagcg ccgaaacggc ttcgtcggca atgatcactt
    12481 ttggattcaa tgccaacgcg cgagcaatgc agatgcgctg gcgctgaccg ccggaaaact
    12541 catgcgggta acgccaggca tgttcaggta acaggcccac gcgctccagc aaccacgcaa
    12601 cgcgtgcagc cgcgtcttta cctggcaata aaccgtgtac acgcagcggt tcgataatcg
    12661 aatcaccgat ggtctgacgt gggtccagcg aagcgtaagg gtcctgaaaa ataaactgaa
    12721 tatcccggcg caatgcctga agtttgccag gtgacaaggt atcgattcgc tgaccgttaa
    12781 agataatttc gccgccctgc gattcgacca ggcgcagcaa cgcccgcccg gtagtggatt
    12841 taccgctgcc agactcgccc accagcgata gcgtttcgcc aggccagaga tcaaaactga
    12901 ctttctcaac ggcatgcact tcccgcgtta cgcgattcaa caaaccgctg cgcaaaggga
    12961 aacgggtgac aagattacgc acccgtaaaa caggttcgcc atccaccacc gttttctgct
    13021 cgatgggggg ttcctgtttc gctggatgtt caagcgatat caatgggaaa cgtcggggat
    13081 aatctaaccc tttcatcgca ccaagttgcg gaacagcagc taacagcgca cgggtgtaag
    13141 gatgttgcgg tgcatgaaaa atctgttcga cggtacccgt ttccaccgcc tcgccctgat
    13201 acatcaccag tacccgatcg gcaatctctg ccaccacgcc catatcgtga gtgataaaga
    13261 taacgcccat cgacatctct ttttgcaata ctttgattaa ttgcaggatc tgcgcctgaa
    13321 tagtgacatc cagcgcggtg gttggctcat cggcaatcag caccgccggg cggcatgaca
    13381 gcgccatcgc aatcatcact cgctggcgca tcccgccaga gagttgatgc gaataacgtg
    13441 aaagaatggt ttgtgcctcc ggaatgcgta cctgatccag catccgcttc gcctcgacca
    13501 tcgcttcttc acgactggcg ttctgatgca gacgaattga ttcggcaatc tgttcaccca
    13561 cagtaaatac tgggttcagc gatgtcatcg gctcctgaaa tatcatcgcc atatccgcac
    13621 cgcgcacatg gcgcatttgt gcagcgctct gctcgctaag ttcaatcaca tcacgactgc
    13681 gccgccgcaa cagcatttta tcgcactgta ctaaaccgcc cgcctgttcc aacagacgca
    13741 tcaatgccag cgcagtcact gacttaccgg agccggattc gcctacaatt gccagcgtct
    13801 caccgcgttg cagactaaaa gagagattgc ggaccgcagc tattttctgc tggtcctgca
    13861 taaaggcaat attaagattt tcaaccgcca gcacattacc ggcatcaagt tcatcactgt
    13921 gtggcaacgg tgtccccttt ttcacggtag ataccggtgg ttggcgtatc gcctgcgtag
    13981 ccccaggcgc gatacattcc ttcggtgtta aacggtagcg cgacattccc ttcatggtcg
    14041 atagcgatta agccaccgct accgccaagc gcagggagtt tttccattac tacccgctcg
    14101 caggcttccg cgagacttaa tccgccgtaa tccattaacg cggcgatgtc atatgccgcc
    14161 agcgcgcgga tgaagacttc gcctgtgccg gtacaagaaa ccgccacact ggcgttattg
    14221 gcgtagcatc cggcacccac taagggacta tcgccaactc gtccgggtaa tttattggtc
    14281 attccgcctg tggacgtggc tgccgccaga ttgccgtcta aatccaacgc cacggccccc
    14341 acggtgccca ttttctgttt ttcatccagt ggcgcaccgc tatggtcgag gactgttgcc
    14401 ccttcctcgc gcgctgccag tagttgttca taacgcaaag gcgtggagaa aatctccggt
    14461 gagacgcgct ccatgccatg agcaaacgca aaattttctg ccccttcgcc aatcatcatc
    14521 acatgcgggc tttgctccat caccagccgg gcggcaagaa ccggattacg cagatgacta
    14581 acgcccgcca ccgcaccggc tttcagggtg ttaccatcca tcacacaggc gtccagttca
    14641 tgggtttcat cacgcgtaaa gacagcgcca attccggcgt taaacagtgg acactcttcc
    14701 agcagacgca ccgcttccgt caccacatcc agcgcacttt cgccggcttc cagcattttc
    14761 tgcccggttt caacaatggc agacaacgcc tcgatgtagc gtaattcctg ttgcagactc
    14821 atctgcgcgc ggctaattgc acctgcgcca ccatgaattg caatgactgc tttgcccata
    14881 gtattcgtcc attaagggtg gctcgccact tttgctataa atatcagaat ttttatgaat
    14941 tactcattca atttttgaat atagaaagat ggatatggca tgtaaaggca ggagccaacg
    15001 ctacgcccga ggtggcatac agtctatggc gtttttctga cataataggc aaattcgatt
    15061 ttgcctccgc aggagtgttt tcatggaatt taccaccgga ttgatgtcgc tcgacaccgc
    15121 gcttaatgag atgctttctc gcgttacccc actgaccacc caggaaacgc tgccactggt
    15181 tcagtgtttt ggtcgtattc tggcgagcga tgtcgtttcg ccactggatg tcccggggtt
    15241 tgataactcc gctatggacg gctacgcggt gcgtttagcc gatattgcct ccgggcaacc
    15301 gctgcccgtt gccggtaaat cctttgccgg tcagccatac catggtgaat ggcctgcggg
    15361 tacctgcatt cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag cggtggtgat
    15421 gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt actgctgaag tgcgtagcgg
    15481 gcaaaatatt cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt tcccggcggg
    15541 aactcgcctg actaccgcag agctgccagt aattgcttca ctggggattg ccgaagttcc
    15601 ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc agttgcccgg
    15661 tcagccgctg ggcgacggcc aaatctacga caccaaccgt ctcgccgtac acctgatgtt
    15721 agaacagctg ggttgcgagg taatcaactt agggattatc cgcgacgatc cacatgccct
    15781 gcgcgccgca tttattgaag ccgacagcca ggcggatgta gtgatcagtt ccggcggtgt
    15841 ttcagtgggt gaagcggatt acaccaaaat gattcttgaa gagctggggg agatcgcctt
    15901 ctggaagctg gcgattaaac caggtaaacc gttcgcgttc ggtaaactca gcaatagctg
    15961 gttctgcggc ctgccgggca acccggtttc agcgacgctg accttctatc aactggtaca
    16021 gcctttgctg gcaaaactaa gcggcaacac cgccagcggc ctgcccgcgc gccagcgcgt
    16081 acgcacagcg tcccgcctga aaaaaacgcc aggacgtctt gatttccagc gcggcgtgct
    16141 gcaacgcaac gccgacggtg aacttgaagt gacgaccacc ggacatcagg gttcacatat
    16201 atttagctcc tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc gcggcaatgt
    16261 ggacgtgcgc gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag gcctgtaatg
    16321 gcggaactca gcgatcagga gatgctgcgc tacaaccggc aaatcattct gcgcggtttt
    16381 gattttgacg gtcaggaggc gctaaaagat tctcgtgtgc tggtagtcgg cctcggtgga
    16441 ctcggctgcg cagcttcgca gtatctggca agcgccggtg tcggcaacct gacgctgctc
    16501 gacttcgaca cggtttcgct ctcgaatctg caacgccaga cactgcacag tgatgccacg
    16561 gtcgggcaac cgaaggtgga atccgcccgt gacgccctga cgcggatcaa cccacatatc
    16621 gcgattacgc cagtcaatgc actgctagat gacgcagaac ttgcagcatt gattgctgaa
    16681 cacgatctgg tgctcgactg tacggataac gttgcggtac gtaatcaact gaacgcaggc
    16741 tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga agggcaaatc
    16801 acggtcttca cttatcagga cggcgaaccg tgctatcgct gccttagccg tttgtttggc
    16861 gagaacgcgt taacctgcgt agaagcaggc gtgatggcac cgttgatagg cgtaattggt
    16921 tcgttgcagg caatggaagc gatcaaactg ctggcaggtt atggcaaacc ggccagcggg
    16981 aaaatcgtca tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact gatgcgcaat
    17041 ccggggtgtg aggtgtgcgg gcagtaattg ccacctccaa gcctgataag acgcgacaag
    17101 cgtcgcatca ggcatttgct ccagacgcca gatgcggcgt gaacgcctta tctggcctac
    17161 ggttcgggca cagatttcca agcgcggcag gcatcggtgc actccgccac gtcatcagca
    17221 atttatgagg atgcagaacg tccggttaaa tcgacgttct gccaaacgct ccctgccagt
    17281 cctgctcaaa cttcgtcaca gcggcttcca ccgccggata gctaatcatc tgttgtgcca
    17341 catccagtgg cagagtaatt gattcacatc cggccagtaa gcagtccagc gcctgacgcg
    17401 gggttttgaa actcgctgcc agcactttcg cctgcggcgc atgcattttc aataactggt
    17461 gtaagtcggt cacagtctga atgccgctac cgccctgagc atcaatacga ttaacgtaag
    17521 gcgcaacata ttccgcacct gccagcgccg acagcagccc ttgtgccgcg ccatataccg
    17581 cggttcccag cgtcggaatc ccttccgctt ttaacatctt aatcgctgcc agcccctcgg
    17641 ctgtcaccgg aactttcacc acgatatccg caataataga acgcagctta agcgcgtcat
    17701 tcaccatccc ttcagcagtg gtagccatta cctgggcaaa cagacgcccc tgaccgccca
    17761 tcgcttcatg aagttgcgga agcacaacat ccagcggttt tttacccgcg gcgataatgc
    17821 ttgggttagt ggtcacaccc gccagcggaa aaatacgtga cagcgccttc accgcaacaa
    17881 cgtctgaagt atccagatac agttccataa ccatcctcaa aatgtacttt ttgtctgaat
    17941 gtagactatc acgggaaaac tctctgaaca gttgacgcac atcaatctaa ctttcattcg
    18001 aaagtatttt aatgtttgaa cgaaacagag aggcaactat gattttcaat attcagcgct
    18061 actcgaccca tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc tgttcgctgg
    18121 gctgccgctg gtgtcagaac ccggaaagcc gcgcccgtac gcaggatctg ctgtatgacg
    18181 cacgactgtg tctggaaggc tgcgagctgt gcgctaaggc cgcgccggaa gtgattgagc
    18241 gcgcgctgaa tggtttgctt attcatcggg aaaagttaac cccggagcat ctgacggcgt
    18301 taaccgactg ctgtccgaca caggcgttaa ccgtgtgtgg tgaagtgaaa agcgttgagg
    18361 agatcatggc gaccgttctg cgcgataaac cgttttacga tcgcagcggc ggcggcttaa
    18421 cgctttcggg tggtgaaccc ttcatgcagc cggaaatggc gacggcgctg ctgcaagcct
    18481 gccacgaggc aggcattcat actgcggtag aaacctgtct gcatgtgccg tggaaatata
    18541 tcgccccttc tctgccctat atcgatctgt ttcttgccga tttaaaacac gttgccgacg
    18601 cgccgtttaa acagtggacc gacggtaacg ccgccagggt gctggataac ctgaaaaaac
    18661 tcgccgcagc gggcaaaaaa atcattatcc gcgtgccgct gattcagggc tttaatgccg
    18721 acgaaacctc tgtaaaagcc attaccgatt ttgccgccga cgagctgcac gttggcgaaa
    18781 ttcattttct gccctaccac acgctgggca tcaacaaata tcacttactt aatctgccct
    18841 atgacgcccc ggaaaaaccg cttgatgcgc cagagctgct cgattttgcc cagcagtatg
    18901 cctgccagaa agggttaacc gcgaccttac gaggataaca accatgacca cactgaaact
    18961 ggacacgctc agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata ttgtgaagcc
    19021 gccagtctgt accgagcgcg cgcagcacta taccgagatg tatcaacaac atctcgataa
    19081 gccgatcccg gtacgtcgcg cgctggcact ggcgcatcac ctggcgaatc gcaccatctg
    19141 gatcaaacac gatgagttga tcattggcaa ccaggcaagc gaagttcgcg ccgcgccgat
    19201 cttcccggaa tatactgtct cgtggatcga aaaagagatt gatgatctgg cagatcgtcc
    19261 cggtgctggc tttgcggtga gcgaagagaa caaacgtgtt ctgcatgaag tgtgcccgtg
    19321 gtggcgcggt cagacggtac aggatcgctg ctatggcatg tttaccgatg agcaaaaagg
    19381 tctgctggcg accggcatca ttaaagcgga aggcaatatg acctccggcg atgcgcacct
    19441 ggcggtcaat ttcccgctgc tgctggaaaa agggcttgat ggtctgcgtg agaaagtagc
    19501 ggaacgtcgc tcgcgcatca atctgacggt gctggaagat ttgcacggcg aacagttcct
    19561 gaaagcgatt gatatcatgc tggtggcagt cagtgaacac attgaacgtt tcgctgccct
    19621 ggcgcgtgca atggccgcga ccgaaacccg cgaaagccgt cgcgatgaac tgctggcgat
    19681 ggcagaaaac tgcgatctta tcgcccacca gccgccgcag actttctggc aggcgctgca
    19741 actgtgttac ttcatccagt tgattttgca gattgaatct aacggtcact cggtgtcgtt
    19801 tggtcgtatg gatcagtatc tctacccgta ctatcgccgc gacgttgaac tgaaccagac
    19861 gctggatcgc gaacacgcca tcgaattgct gcatagctgc tggttgaaat tgctggaagt
    19921 gaacaaaatc cgctccggct cccactcaaa agcctctgcg ggaagtccgc tgtatcagaa
    19981 cgtcaccatt ggcggacaaa atctggttga tggtcaacca atggacgcgg tgaatccact
    20041 ctcttacgcg atcctcgaat cctgcggtcg cctgcgttcc actcagccta acctcagcgt
    20101 gcgttaccat gcaggaatga gtaacgattt cctcgacgcc tgcgtacatg tgatccgctg
    20161 cggcttcggg atgccggcat tcaacaacga cgaaatcgtg atcccggaat ttattaaact
    20221 cggtattgaa ccgcaggacg cttatgacta cgcagcgatt ggttgtatag aaaccgccgt
    20281 cggtggcaaa tggggctatc gctgtaccgg catgagcttt atcaacttcg cccgcgtgat
    20341 gctggcggcg ctggaaggcg ggcgtgatgc caccagcggc aaagtgttcc tgccacaaga
    20401 aaaagcgttg tcggcaggta acttcaacaa cttcgatgaa gtgatggacg cgtgggatac
    20461 gcaaatccgt tactacacgc gtaaatcaat cgaaatcgaa tatgtcgtcg acaccatgct
    20521 ggaagagaac gtgcacgata ttctctgctc ggcgctggtg gatgactgta ttgagcgagc
    20581 gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc aggtcggtat
    20641 tgccaacctc ggcaacagcc tggcggcagt gaagaaactg gtgtttgaac agggcgcgat
    20701 tggtcagcaa cagctggctg ccgcactggc ggatgacttc gatggcctga ctcatgagca
    20761 actgcgtcag cggctgatta acggtgcgcc gaagtacggc aacgacgatg ataccgtcga
    20821 tacgctgctg gctcgcgctt atcagaccta tatcgacgaa ctgaaacagt accataaccc
    20881 acgctacggt cgtggtccgg ttggcggcaa ctattacgcg ggtacgtcgt ctatctccgc
    20941 taacgtaccg tttggcgcgc aaactatggc aacgccggac gggcgtaaag cccacacccc
    21001 gctggcagaa ggcgctagtc cggcctctgg tactgaccat ctcggcccta ctgcggtcat
    21061 tggctcagtg ggtaaactgc ctacggcagc gattctcggc ggcgtgttgc tcaaccagaa
    21121 actgaatccg gcaacgctgg agaacgaatc tgacaagcag aaactgatga tcctgctgcg
    21181 taccttcttt gaagtgcata aaggctggca tattcagtac aacatcgttt cccgcgaaac
    21241 gctgctggag gcgaaaaaac atccggatca gtaccgcgat ctggtagtgc gtgtcgcggg
    21301 ttattccgcc ttcttcaccg cgctctctcc agacgctcag gacgatatca tcgcccgtac
    21361 tgaacatatg ctgtaatctc ttcggcccgg ctgcggcgtc gccgggctaa atcattcaca
    21421 tcatcaattt catccttact ttcattcgaa atataatttg tgctctgcgt cacattgtta
    21481 ttacactgtt acggtttcat tttcgagcca ggagcacacc tatgagcgta aaagttatcg
    21541 tcacagatat ggacggtact tttcttaacg acgccaaaac gtacaaccaa ccacgtttta
    21601 tggcgcaata tcaggaactg aaaaagcgcg gcattaagtt cgttgtcgcc agcggtaatc
    21661 agtattacca gcttatttcg ttctttcctg agctaaagga tgagatctct tttgtcgcgg
    21721 aaaacggcgc actggtttac gaacatggca agcaactgtt ccacggcgaa ctgacccgac
    21781 atgaatcgcg gattgttatt ggcgagttgc taaaagataa gcaactcaat tttgtcgcct
    21841 gcggtctgca aagtgcatat gtcagcgaaa atgcccccga agcatttgtc gcactgatgg
    21901 caaaacacta ccatcgcctg aaacctgtaa aagattatca ggagattgac gacgtactgt
    21961 tcaagttttc gctcaacctg ccggatgaac aaatcccgtt agtgatcgac aaactacaca
    22021 tagcgctgga tggcattatg aaacccgtca ccagtggttt tggctttatc gacctgatta
    22081 ttcccggtct acataaagca aacggtattt cgcggttact gaaacgctgg gatctgtcac
    22141 cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg aaaatggcgc
    22201 gttattcctt tgcgatgggc aatgctgcgg aaaacattaa acaaatcgcc cgttacgcta
    22261 ccgatgataa taatcatgaa ggcgcgctga atgtgattca ggctgtgctg gataacacat
    22321 ccccttttaa cagctgacct ctctaccgga gccatgcgct ccggttttcc cttcttttta
    22381 catcctgtac atcacatcaa atcattaaac ttgcattaac ttatttataa cttgtattgt
    22441 cacatgcaaa ttagcatagt ttcgaatgaa agttaatgca aggtggttat ggcttctact
    22501 tttaccagcg acacattgcc tgccgatcac aaagcagcta tccgtcagat gaagcacgcg
    22561 ctgcgggcgc agcttggcga tgtccagcag atctttaatc agctaagcga tgacattgcc
    22621 acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg gccggtgctg
    22681 tcttatgccg atatcaaagc aggtcatgtt actgcagagc agcgcgaaca gattaaacgt
    22741 cgcggttgtg cggtgataaa aggccatttc ccccgcgaac aagcgctagg ctgggatcag
    22801 tcgatgctgg actatctgaa ccgcaaccgc tttgacgagg tctacaaagg ccccggcgat
    22861 aatttcttcg ggacgctcag cgcttcacgt cccgagattt accccatcta ctggtcgcag
    22921 gcgcaaatgc aggcccgcca gagtgaagaa atggcgaatg cgcagtcgtt tctcaatcgt
    22981 ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag cgtcatctac
    23041 cctgaccgta tccgccgcct tccgcccgga acgacctcca aaggtcttgg agcgcatacc
    23101 gactccgggg cgctggaacg ctggctgctt ccagcgtatc agcacgtttt cgccaacgtc
    23161 tttaatggca atctggcgaa atacgatccc tggcatgcgg cacatcgtac ggaagttgaa
    23221 gagtacacgg tggacaacac caccaaatgt tccgtgtttc ggacattcca gggctggact
    23281 gcgctctcgg atatgctgcc tggtcagggg ttgctgcacg tcgtgcccat tcctgaagcc
    23341 atggcgtacg tactgttacg tccgctgctt gatgatgtgc cggaggatga actgtgcggc
    23401 gtagcgcccg gaagagtgtt gccggtatca gagcaatggc atccactgtt aattgaggcg
    23461 ttaaccagca ttccaaaact cgaggccgga gactccgtct ggtggcactg cgacgtcatc
    23521 cattccgttg cccccgttga aaatcaacag ggttggggca acgtgatgta cattcctgcg
    23581 gcaccgatgt gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc actggaaaaa
    23641 ggcgcatcgc cgggcgactt cccgcgcgag gactatgaaa caaactggga aggacgcttt
    23701 acgcttgccg acctcaacat tcacggtaag cgagcgttgg gcatggatgt ttgagagtta
    23761 atggaagtga attgatgtgg gattaagaaa atcgattcga cgttactcag attgctgaca
    23821 agtgcgcttt cttaatgcct gatgcggcgt taacgcctta tccggcctac aaagtcctgc
    23881 agattcaata tattacaggt actgcgtagg cctgataagc gtagcgcatc aggctatttt
    23941 gcatttgtca tcagccgtcg tcaccccgac agtatcgggg tgacggataa tcacttactc
    24001 gatcccttta ctacgcaggt aatcttcgta attaccgcta aagtcgatca cgcgttccgg
    24061 ggtgatttcc agaatgcggg tcgccaggga gcttacgaac tcacggtcgt gtgaaacaaa
    24121 gatcagcgtg ccctgataca gttccagcgc catgttcagt gactcgatgg attccatatc
    24181 caggtggttg gtcggttcgt ccatgatcag aatgttcggc ttctgcatca ttaacttacc
    24241 aaacagcatc cgcccttttt ccccaccgga aagcacttta gctggctttt tgatgtcgtc
    24301 ctggctgaac agcaaacgac cgagaatgct gcgtaccgcc tgctcgtcat cgccttcctg
    24361 cttccactga ctcatccatt cgaacacggt cagatcattt tcaaactcat attcgtggtc
    24421 ctgggcgtag taaccaatgc gcgcgttttc agaccatttt acggtgccgc tgtccggttg
    24481 cagatcgccc accagcgttt tcagcagcgt tgatttaccg acgccgttgg tacccagtac
    24541 cgcaagtttt tcaccgacct ccagcagcag gttgagattt ttaaacagtg gaccgttatc
    24601 aaaccctttg gtcagacctt ccacttccag cgcgttacgg aacagtttct tatcctgttc
    24661 aaaacggata aacgggttct gacggctgga ggctttcacc tcttccagtt tgattttatc
    24721 aatctggcgc gcgcgcgaag ttgcctggcg agatttcgag gcgttggcgc taaagcggct
    24781 aacgaaagat tgcaactcag caatctgcgc tttcttcttg gcgttatcgg ccagcagacg
    24841 ttcacgcgct tgggtcgccg ccgtcatgta ctcatcgtag ttacccggat aaacgcgcag
    24901 ctcgccgtaa tccagatccg ccatgtgggt acagaccatg ttaaggaagt gacggtcgtg
    24961 cgagatgatg atcatggtgc tgtcacgctc gttcagcacc tgttccagcc agcgaatggt
    25021 gtcgatgtcg aggttgttgg tcggttcgtc gagcaggaga atatccggct cagcaaacag
    25081 cgcctgcgcc agaagcacac gcagcttcca gccaggagca acttcactca tcgggccgta
    25141 gtgctgttcc actggaattc ccacgccaag caacagttca ccggcgcgag cttccgcaga
    25201 gtaaccgtcc atttcgccgt atttcacttc cagatcggcc actttatagc cgtcttcttc
    25261 actcatttcc ggcaaagcat agatgcggtc gcgctcctgc ttcacttccc acaactcttt
    25321 atgccccatg atcaccgtat ccagcacagt gaactcttca aaggcaaact gatcctgacg
    25381 cagcttacca atgcgctcgt tgggatcgag ggaaacgtta cccagcgtcg gctcaaggtc
    25441 gccgccgaga atcttcataa aggtggattt accactaccg ttcgcgccaa tcaggccgta
    25501 acggttgccg ccgccgaatt tgacggaaat gttttcaaac aacggcttac tgccgaactg
    25561 catggtgacg ttactggaaa ctaacacagg cgtatcctga aaagagatat gacaaaccgc
    25621 gcattatgcc ataagtctga aagaagatcc tgctcctcgc ttaaggtcta cactcaagcc
    25681 aaaggcaaaa aatgtgattt cgtacacatc tgatttcact gtgagctgga atgaacttat
    25741 aatgcgcttc caatactcta atattctcaa cccaatggcc tgccaggcac aaaatctcgc
    25801 ttaacatgaa tatgaaattg aaaacattat tcgcagcggc cttcgctgtt gtcggctttt
    25861 gcagtaccgc ctctgcggta acttatcctc tgccaaccga cgggagtcgc ctggttggtc
    25921 agaatcaggt gatcaccatt cctgaaggta acactcagcc gctggagtat tttgccgcgg
    25981 agtaccagat ggggctttcc aatatgatgg aagcgaaccc gggtgtggat accttcctgc
    26041 cgaaaggcgg tactgtactg aacattccgc agcagctgat cctgccggat accgttcatg
    26101 aaggcatcgt cattaacagt gcagagatgc gtctgtatta ctatccgaaa gggaccaaca
    26161 ccgttatcgt gctgccgatc ggcattggtc agttaggcaa agatacgcct atcaactgga
    26221 ccaccaaagt tgagcataag aaagcaggcc cgacctggac gccgaccgcc aaaatgcacg
    26281 cagagtaccg cgctgcgggc gaaccgctac cggctgtcgt tccggcaggt ccggataacc
    26341 cgatgggtct gtatgcactc tacatcggtc gcctgtatgc tatccatggc accaacgcca
    26401 acttcggtat cggcctgcgt gtaagtcatg gttgtgtgcg tctgcgtaac gaagacatca
    26461 aattcctgtt cgagaaagta ccggtcggta cccgcgtaca gtttattgat gagccggtaa
    26521 aagcgaccac cgagccagac ggcagccgtt atattgaagt ccataacccg ctgtctacca
    26581 ccgaagccca gtttgaaggt caggaaatcg tgccaattac cctgacgaag agcgtgcaga
    26641 cagtgaccgg tcagccagat gttgaccagg ttgttcttga tgaagcgatc aaaaatcgtt
    26701 ccgggatgcc ggttcgcctg aattaatcgt caaaacgtaa aacaaaggcg gactcataat
    26761 ccgccttttt tatttgccag accttaccag gccgggatca taactaacaa aacgtatccc
    26821 accagcgccg cccacaacag catcggtatt gaatagagat ggaaacgcca ccagatgcgg
    26881 cgatcgttcg ccatacgtag cgcaatcaaa tttgccagcg atcctggtaa taaaccaaag
    26941 ccccccacgt ttaccgccca tgccagtaat aaagacggag gtacataatt gagcagcaaa
    27001 atggtactcg gcacgttact gatcacctgc gataaaccga ctgccgttaa ccataacccg
    27061 ggttcagata aatgactcac gttacccaac acgccttgca acgctggaag ctgggtcagt
    27121 aaatggacgt cgataaacat cgccataaac accagcagca acgtccagtc cacactcaac
    27181 accacgcgac gcgccagcag cacaaagccc gccgccacaa tcaccagtcc ccacagctct
    27241 tgtttgaact ccagcgccgt cagaaagacg atatacagcc ccagacaatt ccacaccagc
    27301 cgcggtttcc actccggtgt ttgcaccccc gtatggtatt gcagcgcctt tccagggaaa
    27361 caacaccagc acaacagcag gagtgtcagc atcattgcgc cagccatcgg tgccatttgg
    27421 gcaataaatc cggcaaacga aagaccagaa cgtccccaga taagaatatt ttgcgggttg
    27481 ccaattggcg tcagtagcga accagcgttg actgccagcg cctcaaaaat aatcagccga
    27541 ttaaccggga tctcacacag tctttttagc gtgatagtca gcggaacaac aataaacagc
    27601 gcgacatcgt tggtcagaaa ggtagaaagc agcgccgccg ccagcaccat aaacatcgcc
    27661 agccgacgct ccgtagcaaa gcggcgcacc attttgcgcc ccagcacatc aaaataaccg
    27721 cttaactcca cacctttggt cagcagcatc aggccgctta aggtgatgat ggtgtgccag
    27781 tcgatagcag caggccagga tttcggtgca aagggcacga aaaagcttaa tccgatacca
    27841 acaagaatta ataactgaaa aaaacgatcg ccttgcagcg tgcgtaaaaa aggcaggctc
    27901 atttggcacc gtgtttctgg gtaaacaaac gaaaagcgtc gagcgtctct tcactaacat
    27961 ggtgctccat gccttccgcg tcgcgacggg cgatttccgg actgacgccc aacaccaaca
    28021 agaaattttc gactatctga tgtcgctcgc ggctttcctg cgccagcttc tctccttctg
    28081 ccgttaaaaa cacgccacgc caggggatca tttcaatcag ccccatggtt gccagccgct
    28141 taagcatttt agccaccgtc ggttgcgaaa ctcccagacg agcagccatg tccacctgac
    28201 gagcttcccc cacttccctg atcaagtcag aaatcagctc aacgtaatca tcaataagct
    28261 cgcgccgatg cgcctctctg acctggcgga acccttcaac gtgctcttcc acgttcacta
    28321 actgcgtcac tttttttgct attggcgtac ctgcgcgacg actcattgtg cttcctcatt
    28381 atggtgacgc atacatagcg ttctcatttc gtgcgcacat tgtaaaccag agttgcgaag
    28441 gtacaaaaaa ttaacgtttt agcaatagct atataatata gcctgtgcta tatctgtatg
    28501 taatgcaatc atccctcaag gatcgacggg attagcaagt caggaggtct tatgaatgag
    28561 ttcaagaggt gtatgcgcgt gtttagtcat tctcccttta aagtacggtt aatgctgctc
    28621 tctatgttgt gcgatatggt caacaacaaa ccgcagcaag ataaaccttc agataaatag
    28681 cggcgtcgcg gtacgccgct tcactcctgc ttttatgcag gcataacgcg ttttggtctg
    28741 aaaaaaacca ctttttgtcg gatttgcaat ccccttcgca aaagatttgt tcgtcagtag
    28801 ttgacctgaa cggcggctcg ctctatcttc ttgcagccct gcgtatattg cggctcgcgg
    28861 atgcggaccc ctttcccctc ttcacgcact cttgcaggta ttgacccttg acgccagggt
    28921 aagcacatgg cgtttgttac gatagtggca tatgaattta accctcaaag aatcgcttgt
    28981 tacccgtagc cgggtattta gcccgtggac tgcgttctac tttttacagt cgctattaat
    29041 taacctcggc ttaggttacc ccttcagttt gctctacacc gctgcgttta cggctatttt
    29101 gcttttgcta tggcgaacat tgcctcgcgt acaaaaagtt ctggtcggtg tcagttcgct
    29161 ggtggcggct tgttatttcc cttttgctca ggcctacggc gcgcctaatt tcaatacatt
    29221 gctggcattg cactccacca atatggaaga gtcgaccgaa atcctgacga tttttccgtg
    29281 gtacagctac ctggtcggct tatttatttt tgcgctcggc gtaatagcaa tcaggcgaaa
    29341 aaaagagaat gaaaaagcgc gctggaatac cttcgacagc ctgtgtctgg tattcagtgt
    29401 ggcgacattt tttgttgctc ccgtgcaaaa cctggcctgg ggtggcgtat ttaaactgaa
    29461 agatactggc tatccggtat ttcgttttgc taaggatgtc atcgtcaata ataacgaggt
    29521 gattgaagag caagaacgga tggcaaaact ttccggaatg aaagatacct ggacggtcac
    29581 tgccgttaag ccgaagtatc agacctatgt ggtagtgatc ggtgaaagcg cgcgtcgcga
    29641 tgccctcggt gcctttggcg gtcactggga caataccccg tttgccagca gcgttaacgg
    29701 tttgatattt gctgactaca ttgctgccag tggctccacg cagaaatcgc ttggcttaac
    29761 gctcaatcgc gtagtcgatg gcaaaccaca gtttcaggat aactttgtca ccctggcaaa
    29821 tcgcgcgggc ttccagacct ggtggttttc caaccagggc caaatcggcg aatacgatac
    29881 tgctatcgcc agcatcgcca aacgtgcaga tgaagtgtac ttcctgaaag aaggtaattt
    29941 tgaagcagat aaaaacacga aagacgaagc gttactggat atgaccgctc aagtgctggc
    30001 gcaagagcac tcgcaaccgc agctgattgt tctgcatctg atgggctcgc atccgcaggc
    30061 ctgcgacagg acacaaggca aatacgaaac ctttgtgcaa tcgaaagaaa cgtcgtgcta
    30121 tctctatacc atgacgcaaa cggacgattt actgcgcaag ctgtacgatc agttacgcaa
    30181 cagcggcagc agcttctctc tggtttactt ttctgaccac ggtctggcct ttaaagagcg
    30241 cggcaaagac gtgcaatacc ttgcccatga tgataaatat cagcaaaatt tccaggtgcc
    30301 ttttatggtc atttccagcg gcgataaagc gcatcgggtg attaaagccc gccgctcagc
    30361 caatgacttc ttaggctttt tctcgcagtg gacgggaatt aaagcgaagg aaataaatat
    30421 caaatacccg tttatatctg agaagaaagc cgggccgata tacatcacca acttccagtt
    30481 acagaaggtg gattacaacc atctcggaac cgatattttc gacccgaaac cttaaaaaca
    30541 aaaatccgcc ccgagaggcg gatttttata tcaccaaagt gattagaagc ggtaaccaac
    30601 accggcaatc caggtgccta cgtcaacgct acgaatacgg ctctgctcgt aagagaagtc
    30661 cagagcaacg ttttccatcg ggttgaactg cagaccagca ccgtaggaga aaccgtagtc
    30721 gctggtgtcg tgtttgtagg tcgggtattc agtggtctgg aatttaccat aacccacacc
    30781 cactacaccg tagatgcttg cccagtcgtt aatgcggtaa gccggaccag cagtgatgcc
    30841 gtagtactgg tttttgttgt agtcaccaga gcttgcagta cggcttttct cggtgtaagt
    30901 gaaagaaccg atcacaccca gcgggctgtt gtcttcttca tagcggtatt tcaggttgaa
    30961 accgcccatt ttgttcattt ggccctgagc gtcgctctgt gcgtaaccgc cagttacggt
    31021 agaagtcgca gctacggaag tacctgcggt gaaagccaga actgcggcca gtgctgaaag
    31081 acatgcaatt tttttcataa ccacctcaaa tgtgattcaa ataagtccta agttttaaat
    31141 atatcaaaaa ttaatgggaa actcttcgcg atttgtgatg tctaacgggc catttcatgt
    31201 aacagaacgt ttccatacac cgctatccat ctaaatttaa atcacttttt cagagaactg
    31261 cgtaagtatt acgcatgttt tccctgtcat tcatccagat tattcctaat caccagacta
    31321 atgattccat caatcctggc gcattttagt caaaacgggg gaaaattttt tcaacaaatg
    31381 ctcaaccagc attgggtata tccagtacac tccacgcttt acttaagtct agatatttgt
    31441 gggagaaagg atgcctggtt cattacgtaa aatgccggtc tggttaccaa tagtcatatt
    31501 gctcgttgcc atggcgtcta ttcagggtgg agcctcgtta gctaagtcac tttttcctct
    31561 ggtgagcgca ccgggtgtca ctgcgctgcg tctggcatta ggaacgctga tcctcatcgc
    31621 gttctttaag ccatggcgac tgcgctttgc caaagagcaa cggttaccgc tgttgtttta
    31681 cggcgtttcg ctgggtggga tgaattatct tttttatctt tctattcaga cagtaccgct
    31741 gggtattgcg gtggcgctgg agttcaccgg accactggcg gtggcgctgt tctcttctcg
    31801 tcgcccggta gatttcgtct gggttgtgct ggcggttctt ggtctgtggt tcctgctacc
    31861 gctggggcaa gacgtttccc atgtcgattt aaccggctgt gcgctggcac tgggggccgg
    31921 ggcttgttgg gctatttaca ttttaagtgg gcaacgcgca ggagcggaac atggccctgc
    31981 gacggtagca attggttcgt tgattgcagc gttaattttc gtgccaattg gagcgcttca
    32041 ggctggtgaa gcactctggc actggtcggt tattccattg ggtctggctg tcgttattct
    32101 ctcgaccgct ctgccttatt cgctggaaat gattgccctc acccgtttgc caacacggac
    32161 atttggtacg ctgatgagca tggaaccggc gctggctgcc gtttccggga tgattttcct
    32221 cggagaaaca ctgacaccca tacagctgct ggcgctcggc gctatcatcg ccgcttcaat
    32281 ggggtctacg ctgacagtac gcaaagagag caaaataaaa gaattagaca ttaattaaat
    32341 ttacatttct gtatggttat gcataaccat gcagaatttc tcgctacttt tcctctacac
    32401 cgtatttata tatcgaatta tgcaaaagca tatttattcc gaaaattcct ggcgagcaga
    32461 taaataagaa ttgttcttat caatatatct aactcattga atctttatta gttttgtttt
    32521 tcatgcttgt taccactatt agtgtgatag gaacagccag aatagcggaa cacatagccg
    32581 gtgctatact tagtctcgtt aattactggg acataacatc aagaggatat gaaattatga
    32641 gtaccgctaa attagttaaa tcaaaagcga ccaatctgct ttatacccgc aacgatgtct
    32701 ccgacagcga gaaaaaagca acagtagagt tgctgaatcg ccaggttatc cagtttattg
    32761 atctttcttt gattaccaaa caagcgcact ggaacatgcg cggcgctaac ttcattgccg
    32821 tacatgaaat gctggatggc ttccgcaccg cactgatcga tcatctggat accatggcag
    32881 aacgtgccgt gcagctgggc ggtgtagctc tggggaccac tcaagttatc aacagcaaaa
    32941 ccccgctgaa aagttacccg ctggacatcc acaacgttca ggatcacctg aaagaactgg
    33001 ctgaccgtta cgcaatcgtc gctaatgacg tacgcaaagc gattggcgaa gcgaaagatg
    33061 acgacaccgc agatatcctg accgccgcgt ctcgcgacct ggataaattc ctgtggttta
    33121 tcgagtctaa catcgaataa atccatcgct gatggtgcag aactttagta cccgataaaa
    33181 gcggcttcct gacaggaggc cgttttgttt tgcagcccac ctcaacgcac ttatttagtg
    33241 catccatctg ctatccccag ctgattaagt aaattttttg tatccacatc atcacacaat
    33301 cgttacataa agattgtttt ttcatcaggt tttacgctaa ataatcactg tgttgagtgc
    33361 acaattttag cgcaccagat tggtgcccca gaatggtgca tcttcagggt attgccctat
    33421 aaatcgtgca tcacgttttt gccgcatctc gaaaaatcaa tgagttgcaa aattggcacg
    33481 attttttcat atatgtgaat gtcacgcagg ggatcgtccc gtggatagaa aaaaggaaat
    33541 gctatgaagt ctgtattaaa agtttcactg gctgcactga ccctggcttt tgcggtttct
    33601 tctcatgccg cggataaaaa attagttgtc gcgacggata ccgccttcgt tccgtttgaa
    33661 tttaaacagg gcgataaata tgtgggcttt gacgttgatc tgtgggctgc catcgctaaa
    33721 gagctgaagc tggattacga actgaagccg atggatttca gtgggatcat tccggcactg
    33781 caaaccaaaa acgtcgatct ggcgctggcg ggcattacca tcaccgacga gcgtaaaaaa
    33841 gcgatcgatt tctctgacgg ctactacaaa agcggcctgt tagtgatggt gaaagctaac
    33901 aataacgatg tgaaaagcgt gaaagatctc gacgggaaag tggttgctgt gaagagcggt
    33961 actggctccg ttgattacgc gaaagcaaac atcaaaacta aagatctgcg tcagttcccg
    34021 aacatcgata acgcctatat ggaactgggc accaaccgcg cagacgccgt tctgcacgat
    34081 acgccaaaca ttctgtactt catcaaaacc gccggtaacg gtcagttcaa agcggtaggt
    34141 gactctctgg aagcgcagca atacggtatt gcgttcccga aaggtagcga cgagctgcgt
    34201 gacaaagtca acggcgcgtt gaaaaccctg cgcgagaacg gaacttacaa cgaaatctac
    34261 aaaaaatggt tcagtactga accgaaataa taacgctaca cctgtaaagc gcactggcag
    34321 ttccctctcc cccctgggga gaggattagg gtgaggggcg caaacccgct ccggggccat
    34381 taattaccct gaatttgatt atttacacca cggtaacagg aacaacatat gcagtttgac
    34441 tggagtgcca tctggcctgc cattccgctt ctgattgaag gtgccaaaat gaccctgtgg
    34501 atttcggtcc tcggtctggc tggcggtctg gtaatcggat tgctggcagg ttttgcacgc
    34561 accttcggtg gttggatagc caaccacgtc gcgctggtct ttattgaagt gatccgcggc
    34621 acacctatcg tcgtccaggt gatgtttatc tatttcgccc tgccgatggc gtttaacgac
    34681 ttacgcatcg acccatttac tgcggcggtg gtcaccatca tgatcaactc cggcgcgtac
    34741 attgcggaaa tcacgcgtgg tgcggtgctg tctatccaca aaggttttcg tgaagcagga
    34801 ctggcgctcg gtctttcacg ttgggaaacc attcgctacg tcattttacc gctggcactg
    34861 cgtcgtatgc tgccgccgct gggtaaccag tggatcatca gcattaaaga cacctcgctg
    34921 tttattgtta tcggcgtggc ggaactgacc cgtcaggggc aagaaattat tgccggtaac
    34981 ttccgcgccc ttgagatctg gagcgccgtg gcggtgttct atctgattat taccctggtg
    35041 ctgagcttta ttctgcgtcg tctggaaaga aggatgaaaa tcctgtgatt gaatttaaaa
    35101 acgtctccaa gcactttggc ccaacccagg tgctgcacaa tatcgatttg aacattgccc
    35161 agggcgaagt cgtggtgatt atcgggccgt ccggttccgg taaatcgacc ctgctgcgct
    35221 gcatcaacaa actggaagaa atcacctccg gcgatctgat tgtcgatggc ctgaaggtca
    35281 acgatccgaa agttgacgag cgcctgattc gccaggaagc aggtatggtg ttccagcagt
    35341 tttacctctt cccgcatctg acggcgctgg gaaacgtcat gtttggtccg ctacgcgtgc
    35401 gtggcgcgaa caaagaagag gcggaaaaac tggcgcgtga gctgctggcg aaagtcggtc
    35461 tggcagaacg tgcacatcac tacccttccg aactttctgg tggtcaacag cagcgtgtgg
    35521 cgattgcccg cgcgctggcg gtgaagccga aaatgatgct gtttgatgaa ccgacttccg
    35581 ctcttgaccc ggaactgcgc catgaagtgc tgaaggttat gcaggatctg gctgaagaag
    35641 ggatgacgat ggtgatcgtg acccacgaaa tcggttttgc cgagaaagta gcttcgcggc
    35701 tgatctttat cgacaaaggc cggattgcgg aagatggcaa tccgcaggtg ttgatcaaga
    35761 acccgccgag ccagcgcttg caggaatttt tgcagcacgt ctcttaataa gacacattgc
    35821 ctgatgcgct acgcttatca ggcctacagg atatctggca acttattaaa attgcatgaa
    35881 cttgtaggac ggataaggcg ttcacgccgc atccggcaaa aaagcccgca cgttgtcagc
    35941 aacctgctta atatcccttc ctccctttca cccgaaaggg aggcacacca gattcctctc
    36001 atttaaaatc gcccctcccc cagcatctat acttatcttt ttgctctgtt ttctcactgg
    36061 aggagtcatg cggtggatcc tgttcatcct cttctgcctg ctgggcgcac ctgcccacgc
    36121 ggtatccata cccggcgtta caaccacaac gacaacggac tcaacgactg aaccggcccc
    36181 ggaaccggat atcgaacaaa aaaagcggcc tatggcgcac tggcggatgt gctggataac
    36241 gacacctcgc gtaaagagtt gatcgaccag ttgcgtactg tcgccgcgac gccccctgcg
    36301 gaaccggtcc cgaagatcgt gccgccgacg ctggttgaag agcaaaccgt gctgcaaaag
    36361 gtcaccgaag tcagccgcca ttatggtgaa gccctttccg cccgcttcgg gcaactttat
    36421 cgcaatatca ccggctcccc gcataagcca tttaatccac aaaccttcag caatgcgctg
    36481 agccattttt caatgttagc ggtattagtg tttggttttt actggctgat tcgcctgtgc
    36541 gcactgccgc tatatcgcaa aatgggtcag tgggcgaggc aaaaaaatcg tgagcgcagc
    36601 aactggttgc agcttccggc gatgattatt ggggcgttta ttatcgacct gctgttactg
    36661 gccctgacat tatttgtcgg ccaggtatta agcgacaacc tgaatgcagg cagtcgcacc
    36721 atcgctttcc aacaaagttt gtttctcaac gcctttgccc tgattgaatt tttcaaagcc
    36781 gtactacgcc tgattttttg cccaaacgtg gcggagctgc gcccgttcac gattcaggac
    36841 gagagcgccc gttactgggg tcgtcgcctg agctggttaa gcagcctgat tggctatggc
    36901 ctgattgtgg ccgtgccgat tatctctaat caggtgaatg tacagatagg tgcgctggcg
    36961 aacgtcatca ttatgctgtg catgaccgtc tgggcgttgt acctgatctt ccgaaataaa
    37021 aaagagatta cccagcatat gctcaacttt gcggagcatt cgctggcttt tttcagcctg
    37081 tttatccgcg cctttgcgct ggtgtggcac tggctggcaa gcgcctattt tatcatgctg
    37141 tttttctttt cgttgttcga tccgggcaac agtctgaaat ttatgatggg tgcaacggtg
    37201 cgcagcctgg cgattattgg tatcgcagcg tttgtttccg gtatgttttc ccgctggctg
    37261 gcgaaaacca tcaccctctc gccacatact cagcgtaact atccggagct acaaaaacgg
    37321 ctgaatggct ggctttcggc ggcgctgaaa acggcgcgta ttctgaccgt ctgcgtggca
    37381 gtaatgttgc tgttgagcgc atggggattg ttcgatttct ggaactggct gcaaaacggc
    37441 gcggggcaga aaaccgtaga tatcctgatc cgcatcgcac tcattctttt cttctcggcg
    37501 gttggctgga cggtgctcgc cagtttgatc gaaaaccggc tggcttcgga tattcatggc
    37561 cgcccgctac ccagcgcccg tacgcgtacc ctgctgacgc tgttccgtaa cgcgctggcg
    37621 gtgattatca gtaccatcac catcatgatt gtgttgtcgg aaatcggcgt caatatcgcg
    37681 ccattgctgg caggtgccgg ggcattaggt ctggctatct cgtttggttc gcaaacgctg
    37741 gtaaaagata ttatcaccgg ggtatttatt cagtttgaaa acggcatgaa cactggagat
    37801 ttggtgacta tcgggccgtt gaccggcaca gtggaacgga tgtcgattcg ctccgtgggc
    37861 gtgcgccagg ataccggggc gtatcacatc attccgtggt cttcgataac cacctttgct
    37921 aacttcgtcc gcggtattgg ttcggtagtg gcaaactacg atgttgatcg ccatgaagat
    37981 gctgataaag ccaatcaggc actgaaagat gcggtaacgg aattgatgga aaacgaagaa
    38041 attcgcgggc tgattattgg tgaaccgaac tttgccggga ttgtcggctt aagcaatacc
    38101 gcgtttacac tgcgtgtttc gttcaccacg ctgccactca aacagtggac ggtacgcttt
    38161 gccctcgaca gccaggtgaa aaaacatttc gacctggcgg gcgttcgcgc gccagtgcag
    38221 acttatcagg tgctgcctgc tccgggcgcg accccggctg agccgctgcc gccgggggag
    38281 ccaacgcttt aacgttggcg attaacaaaa cggcggcgct gctcgtcgtt cataaaggtc
    38341 caggcaataa agcgactctg cttttgcccc tgggccatct ctttttttac caccttcacc
    38401 gcgcccacgt cggtcagagc acgatacaac ggcggtaagt tttcaccacg agataccagc
    38461 gaggtaaacc acatcacctg cttcgcgaag cctttgctct cttcaatcat ctttttgata
    38521 aagttgactt caccgccttc acaccacaac tcctgttgct gaccaccaaa gttcagtgca
    38581 tcgtctttgt tcagccccag gttacggcgt ttacgctcac tacctgcccg cgccgcagcg
    38641 gcggaatcgt ggaatggcgg gttacacaag gtcgcgtcgt attgctcgtt tttgtggatg
    38701 atgccgttaa aaatcgcccc actctctttt tgccgacgca gacgaatggc gcggttaagc
    38761 cccggattag cactgataat cgcctgcgca ctgcttaacg cctggctgct ggtttcacta
    38821 ccggtaaaac gccagccata ttcatgtacg ccaatcagcg gataaataca gttcgcacca
    38881 acgccgatat ccagaatgct ggcattcgct ggaattgttc cgcttgcttc agccagtaaa
    38941 tcggcaaggt gatgaatata atccgcccgg cccggtactg gtgggcagag aaaaccgtcg
    39001 gggatatccc agttcgctac ggcgtaaaaa tgggccagca acgccttatt gagcgccttc
    39061 accgccagcg gattggcaaa gtctacgctt tgctcccctg cgggtgtaag cgtgaggaat
    39121 tgcctgagtt caggattgac ctgacaaagc gtggcgagat cgtagcggct gtgatgacgg
    39181 ttgcgcggat gcaaccccgg tttctgggcg gacatagcat tctcctttaa gcagcggcgt
    39241 aagatacccg cataacgcga cagggtaaat atctggctgc gtttatcatt ccccaaaacc
    39301 ttaacatggc tatggtttat ggcaattcat cataaaaatc agcattatgc atgtcattga
    39361 tttgtcttat tttgtaaaaa tatatcgttc aaaaaataag catttctctg tcaatttttt
    39421 gcgtcatttg catcgcgcag gcgacctaca cttatcaggc actacccgtt gtatttatga
    39481 ggatgtcatt atgaaaaagt gcctcacact actgattgcc accgtcctga gcggaatctc
    39541 tctcacggct tatgccgcgc aaccgatgag taacctggac agcggtcagc tacggcccgc
    39601 cggtaccgtt tcggcaactg gcgcatcaaa cctaagcgat ctggaggata aactggcaga
    39661 aaaagcgcgc gaacaaggcg cgaaaggtta tgtcattaat tccgcaggcg gaaatgacca
    39721 gatgttaggt actgcaacca tctacaaata accccaccaa cgtaactgca ttcgcctctg
    39781 gatgcagttt tcgcctgttt tgtaatcaaa aaacatcaaa tatgaacgca atgtaaataa
    39841 atgtatttct ttttcgcgca atgggtgata gaaaatcgca tcaagtgata atgcttatca
    39901 aaattattat cacttgcacg aacactatca cgggattaac agtggcatcg catccgcaga
    39961 gatgctttct cgtggcagtg aaaatttcaa catataagaa aaagtcacct gcaaaatgga
    40021 aaacaatcgc aatttccctg ccagacaatt tcattcgctc acgttcttt
//
LOCUS       DAJTUP010000027        39106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTUP010000027 DAJTUP010000000
VERSION     DAJTUP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.027"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..155
                     /locus_tag="OO468_001451"
     CDS             <1..155
                     /locus_tag="OO468_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6258617.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO468_001452"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO468_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR6258618.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO468_001453"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO468_001453"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR6258619.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO468_001454"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO468_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO468_001455"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO468_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR6258620.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO468_001456"
     CDS             complement(5626..7134)
                     /locus_tag="OO468_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6258621.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO468_001457"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO468_001457"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR6258622.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO468_001458"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO468_001458"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6258623.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO468_001459"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO468_001459"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR6258624.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO468_001460"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO468_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR6258625.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO468_001461"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO468_001461"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR6258626.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO468_001462"
     CDS             complement(14283..15602)
                     /locus_tag="OO468_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR6258627.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO468_001463"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO468_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR6258628.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO468_001464"
     CDS             16699..17034
                     /locus_tag="OO468_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR6258629.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO468_001465"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO468_001465"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR6258630.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO468_001466"
     CDS             complement(17790..19172)
                     /locus_tag="OO468_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6258631.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO468_001467"
     CDS             complement(19358..20302)
                     /locus_tag="OO468_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6258632.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO468_001468"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO468_001468"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR6258633.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO468_001469"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO468_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR6258634.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO468_001470"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO468_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR6258635.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO468_001471"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO468_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR6258636.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO468_001472"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO468_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR6258637.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO468_001473"
     CDS             complement(25947..26768)
                     /locus_tag="OO468_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR6258638.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO468_001474"
     CDS             complement(26868..26951)
                     /locus_tag="OO468_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258639.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO468_001475"
     CDS             complement(27044..27379)
                     /locus_tag="OO468_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR6258640.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO468_001476"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO468_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30047
                     /locus_tag="OO468_001477"
     CDS             29136..30047
                     /locus_tag="OO468_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6258641.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30182..31402
                     /gene="mlc"
                     /locus_tag="OO468_001478"
                     /gene_synonym="dgsA"
     CDS             30182..31402
                     /gene="mlc"
                     /locus_tag="OO468_001478"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR6258642.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31527..32222
                     /gene="bioD"
                     /locus_tag="OO468_001479"
     CDS             31527..32222
                     /gene="bioD"
                     /locus_tag="OO468_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6258643.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO468_001480"
     CDS             complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO468_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR6258644.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO468_001481"
     CDS             complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO468_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR6258645.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34283..35137)
                     /locus_tag="OO468_001482"
     CDS             complement(34283..35137)
                     /locus_tag="OO468_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6258646.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO468_001483"
     CDS             complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO468_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6258647.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35767..37805)
                     /locus_tag="OO468_001484"
                     /pseudo
     CDS             complement(35767..37805)
                     /locus_tag="OO468_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37866..38975)
                     /locus_tag="OO468_001485"
     CDS             complement(37866..38975)
                     /locus_tag="OO468_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6258648.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
BASE COUNT         9940 a         9474 c        10003 g         9689 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
    29761 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
    29821 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
    29881 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
    29941 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
    30001 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
    30061 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
    30121 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
    30181 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
    30241 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
    30301 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
    30361 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
    30421 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
    30481 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
    30541 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
    30601 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
    30661 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
    30721 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
    30781 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
    30841 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
    30901 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
    30961 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
    31021 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
    31081 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
    31141 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
    31201 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
    31261 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
    31321 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
    31381 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
    31441 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
    31501 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
    31561 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
    31621 aaagggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
    31681 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
    31741 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
    31801 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
    31861 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
    31921 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
    31981 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
    32041 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
    32101 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
    32161 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
    32221 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
    32281 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
    32341 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
    32401 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
    32461 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
    32521 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
    32581 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
    32641 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
    32701 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
    32761 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
    32821 tcgctatgat taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg
    32881 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
    32941 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
    33001 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
    33061 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
    33121 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
    33181 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
    33241 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct
    33301 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
    33361 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
    33421 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
    33481 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
    33541 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgtg cgtaatcgtc
    33601 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
    33661 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
    33721 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
    33781 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
    33841 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
    33901 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
    33961 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
    34021 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
    34081 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
    34141 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg
    34201 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
    34261 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
    34321 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
    34381 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
    34441 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
    34501 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
    34561 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
    34621 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
    34681 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
    34741 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
    34801 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
    34861 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
    34921 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
    34981 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
    35041 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
    35101 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
    35161 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
    35221 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg
    35281 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
    35341 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
    35401 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
    35461 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
    35521 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
    35581 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
    35641 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
    35701 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
    35761 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag
    35821 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
    35881 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
    35941 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
    36001 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
    36061 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg
    36121 acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg
    36181 gcgaggatca catagcccat attccctgca gattcatgag agataaggtc ttcttgctct
    36241 gttggcatca ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg
    36301 tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg
    36361 atgtcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg
    36421 acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg
    36481 tcgatggcat ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc
    36541 gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt
    36601 atgccgacgt taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg
    36661 ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc
    36721 tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc
    36781 ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta
    36841 cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat
    36901 ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca
    36961 tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagctgt gtcgttatag
    37021 cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa
    37081 gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt
    37141 ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt
    37201 gcggcactga tctgtgcggt actgtagctg ccatagcgac tgagaaaacc cccgcaagag
    37261 ttcatcagac ggtacgggac gttcgagttg gtgatatttc cgccatctac gcctgttccg
    37321 tacaggacat gtacagcctc attgccgtaa tctttcagga tccgccgaag attatcactg
    37381 atggtatcca gggcttcgtc ccaacttatc cgttcaaatt taccttcacc gcgctcgccg
    37441 acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg gatagagcgc
    37501 ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac
    37561 tccacccagt acacggtgtc atctttcaca tgcaaacgta gcagacagcg gctcccgcag
    37621 ttaacggtgc aggaactcca gaccgttttc tcttctaccg gagcctctgc cgcccggacc
    37681 atttgggaaa atggcagagt gaaagcactg cttgccagcg cgagactgcc aagtgcggag
    37741 gttttcatca gacttctatg gctgatttca gccttcatga gcgcctctgt ggtatggatt
    37801 ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta
    37861 gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt
    37921 ggtgaagggc gcagagtggt tagcgtattc acacatccgc catggtcgat tttatcgcca
    37981 gacatattgg gctcgtgcca ggctccctgg cccatagcgc taactccagg gagaatgcgt
    38041 ggtgttactt tggctggtag ccgaacttcg ccacgatggt taaacacccg caccatatcg
    38101 ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc ctgacggcag
    38161 gctgccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa
    38221 ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc aaaggttgag
    38281 gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt
    38341 tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc
    38401 tcggggtcgt cacgaaatgc tttgtaggcg acaaaatggc cattgggatc tttacgctta
    38461 tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt
    38521 ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag
    38581 acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg
    38641 aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg
    38701 ttaggaataa tatcttcctg ctcaacggtc atcaggtctt ccagcagaat gtcggcatat
    38761 tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc
    38821 tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt atttcccgcg
    38881 tagttccaga tggactccat tgccagacgg atggatggac ttgaagtagt tgactcaatg
    38941 ctacttaagg tcctgatatg actgacatta atcatgataa cactgtatat ccatccagtt
    39001 ctttctctgg gtctccctat cggtatcagc aagtctacac ctcaagccct gacacaaggc
    39061 tgacacaaat caatctactc agcgtagcta agcctcagtt agtccg
//
LOCUS       DAJTUP010000028        38187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTUP010000028 DAJTUP010000000
VERSION     DAJTUP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.028"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     misc_feature    <1..81
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            128..1027
                     /gene="hisG"
                     /locus_tag="OO468_001486"
     CDS             128..1027
                     /gene="hisG"
                     /locus_tag="OO468_001486"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR6258649.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1033..2337
                     /gene="hisD"
                     /locus_tag="OO468_001487"
     CDS             1033..2337
                     /gene="hisD"
                     /locus_tag="OO468_001487"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR6258650.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2334..3404
                     /gene="hisC"
                     /locus_tag="OO468_001488"
     CDS             2334..3404
                     /gene="hisC"
                     /locus_tag="OO468_001488"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR6258651.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3404..4471
                     /gene="hisB"
                     /locus_tag="OO468_001489"
     CDS             3404..4471
                     /gene="hisB"
                     /locus_tag="OO468_001489"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR6258652.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            4471..5061
                     /gene="hisH"
                     /locus_tag="OO468_001490"
     CDS             4471..5061
                     /gene="hisH"
                     /locus_tag="OO468_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR6258653.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5061..5798
                     /gene="hisA"
                     /locus_tag="OO468_001491"
     CDS             5061..5798
                     /gene="hisA"
                     /locus_tag="OO468_001491"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR6258654.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            5780..6556
                     /gene="hisF"
                     /locus_tag="OO468_001492"
     CDS             5780..6556
                     /gene="hisF"
                     /locus_tag="OO468_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR6258655.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            6550..7161
                     /locus_tag="OO468_001493"
     CDS             6550..7161
                     /locus_tag="OO468_001493"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR6258656.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7347..8324)
                     /gene="wzzB"
                     /locus_tag="OO468_001494"
     CDS             complement(7347..8324)
                     /gene="wzzB"
                     /locus_tag="OO468_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR6258657.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(8470..9590)
                     /gene="ugd"
                     /locus_tag="OO468_001495"
                     /pseudo
     CDS             complement(8470..9590)
                     /gene="ugd"
                     /locus_tag="OO468_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(9840..11246)
                     /gene="gndA"
                     /locus_tag="OO468_001496"
     CDS             complement(9840..11246)
                     /gene="gndA"
                     /locus_tag="OO468_001496"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR6258658.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11355..11714)
                     /locus_tag="OO468_001497"
     CDS             complement(11355..11714)
                     /locus_tag="OO468_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258659.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(11711..12217)
                     /locus_tag="OO468_001498"
     CDS             complement(11711..12217)
                     /locus_tag="OO468_001498"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258660.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12363..12926)
                     /locus_tag="OO468_001499"
     CDS             complement(12363..12926)
                     /locus_tag="OO468_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258661.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13133..14071)
                     /locus_tag="OO468_001500"
                     /pseudo
     CDS             complement(13133..14071)
                     /locus_tag="OO468_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14134..14508)
                     /locus_tag="OO468_001501"
     CDS             complement(14134..14508)
                     /locus_tag="OO468_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR6258662.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(14512..15660)
                     /gene="wzy"
                     /locus_tag="OO468_001502"
     CDS             complement(14512..15660)
                     /gene="wzy"
                     /locus_tag="OO468_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR6258663.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(15680..16585)
                     /gene="rfbG"
                     /locus_tag="OO468_001503"
     CDS             complement(15680..16585)
                     /gene="rfbG"
                     /locus_tag="OO468_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR6258664.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(16578..17468)
                     /locus_tag="OO468_001504"
     CDS             complement(16578..17468)
                     /locus_tag="OO468_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR6258665.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(17498..18754)
                     /gene="wzx"
                     /locus_tag="OO468_001505"
     CDS             complement(17498..18754)
                     /gene="wzx"
                     /locus_tag="OO468_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR6258666.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(18770..19315)
                     /gene="rfbC"
                     /locus_tag="OO468_001506"
     CDS             complement(18770..19315)
                     /gene="rfbC"
                     /locus_tag="OO468_001506"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR6258667.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19320..20198)
                     /gene="rfbA"
                     /locus_tag="OO468_001507"
     CDS             complement(19320..20198)
                     /gene="rfbA"
                     /locus_tag="OO468_001507"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6258668.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20257..21156)
                     /gene="rfbD"
                     /locus_tag="OO468_001508"
     CDS             complement(20257..21156)
                     /gene="rfbD"
                     /locus_tag="OO468_001508"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR6258669.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21156..22241)
                     /gene="rfbB"
                     /locus_tag="OO468_001509"
     CDS             complement(21156..22241)
                     /gene="rfbB"
                     /locus_tag="OO468_001509"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6258670.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(22614..23507)
                     /gene="galF"
                     /locus_tag="OO468_001510"
     CDS             complement(22614..23507)
                     /gene="galF"
                     /locus_tag="OO468_001510"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR6258671.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(23682..25076)
                     /gene="wcaM"
                     /locus_tag="OO468_001511"
     CDS             complement(23682..25076)
                     /gene="wcaM"
                     /locus_tag="OO468_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR6258672.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25087..26307)
                     /gene="wcaL"
                     /locus_tag="OO468_001512"
     CDS             complement(25087..26307)
                     /gene="wcaL"
                     /locus_tag="OO468_001512"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR6258673.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26304..27584)
                     /gene="wcaK"
                     /locus_tag="OO468_001513"
     CDS             complement(26304..27584)
                     /gene="wcaK"
                     /locus_tag="OO468_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR6258674.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(27764..29242)
                     /gene="wzxC"
                     /locus_tag="OO468_001514"
     CDS             complement(27764..29242)
                     /gene="wzxC"
                     /locus_tag="OO468_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR6258675.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29244..30638)
                     /gene="wcaJ"
                     /locus_tag="OO468_001515"
     CDS             complement(29244..30638)
                     /gene="wcaJ"
                     /locus_tag="OO468_001515"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR6258676.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(30693..32063)
                     /gene="cpsG"
                     /locus_tag="OO468_001516"
                     /gene_synonym="manB"
     CDS             complement(30693..32063)
                     /gene="cpsG"
                     /locus_tag="OO468_001516"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR6258677.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32256..33692)
                     /gene="cpsB"
                     /locus_tag="OO468_001517"
     CDS             complement(32256..33692)
                     /gene="cpsB"
                     /locus_tag="OO468_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR6258678.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(33695..34918)
                     /gene="wcaI"
                     /locus_tag="OO468_001518"
     CDS             complement(33695..34918)
                     /gene="wcaI"
                     /locus_tag="OO468_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR6258679.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(34915..35394)
                     /gene="gmm"
                     /locus_tag="OO468_001519"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(34915..35394)
                     /gene="gmm"
                     /locus_tag="OO468_001519"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR6258680.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35394..36362)
                     /gene="fcl"
                     /locus_tag="OO468_001520"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35394..36362)
                     /gene="fcl"
                     /locus_tag="OO468_001520"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR6258681.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36365..37486)
                     /gene="gmd"
                     /locus_tag="OO468_001521"
     CDS             complement(36365..37486)
                     /gene="gmd"
                     /locus_tag="OO468_001521"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR6258682.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(37512..38060)
                     /gene="wcaF"
                     /locus_tag="OO468_001522"
     CDS             complement(37512..38060)
                     /gene="wcaF"
                     /locus_tag="OO468_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR6258683.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38076..>38187)
                     /locus_tag="OO468_001523"
     CDS             complement(38076..>38187)
                     /locus_tag="OO468_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR6258684.1"
                     /translation="KKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10191 a         9578 c         8614 g         9804 t
ORIGIN      
        1 ctggaagaca ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc
       61 accttccggg ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa
      121 taagaaaatg acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag
      181 tgatgactca cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg
      241 cctgatcgcg atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat
      301 tccctgtctg gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga
      361 agaagagctg cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg
      421 tctggatttc ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg
      481 tccgctctcc ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta
      541 tctcgaccag aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc
      601 gccgcgtgcc gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga
      661 agctaacggc ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg
      721 cgatggcgaa atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca
      781 gggtgtgatc caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct
      841 ggatgaagtc atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg
      901 tgatcaacag cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat
      961 ggaaaaactg aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat
     1021 ggagtgatcg ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa
     1081 caacgccagc tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt
     1141 aacgatattc tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg
     1201 aagtttgata aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc
     1261 agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa
     1321 accttccaca ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt
     1381 tgccagcagg tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc
     1441 ccgctcttct caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa
     1501 gtggtgctgt gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc
     1561 gatgtgcagg acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt
     1621 acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa
     1681 gcaaaacgcc aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg
     1741 tcggaagtgc tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg
     1801 ctttctcagg ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat
     1861 atggcgcgtc gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa
     1921 accacccgcc aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc
     1981 gtggagatct ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc
     2041 gaactggtcg atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa
     2101 tcggcaggcg actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc
     2161 acctgttcca gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg
     2221 aaagaaggtt tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg
     2281 accgcccaca aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca
     2341 ccgtgactat tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg
     2401 cgcgtcgtct gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg
     2461 tggagtttaa gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg
     2521 tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg
     2581 gcgcggacga aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca
     2641 tcctctactg cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg
     2701 aatgccgcac agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca
     2761 agctggacgg cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga
     2821 tcaatccgca ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg
     2881 ccgatgaagc ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat
     2941 atccgcacct ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt
     3001 gcggatttac gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct
     3061 acccgctctc gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg
     3121 tcgccatgag cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac
     3181 tgaaagagat cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc
     3241 gctttaaagc ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg
     3301 atcagaataa acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag
     3361 aaagccagcg cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct
     3421 ttttatcgat cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg
     3481 ttttgacaaa ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc
     3541 gggctacaag ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca
     3601 agcggatttt gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca
     3661 gtttgatgaa gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc
     3721 gaaagtaaaa ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta
     3781 tgtaattggc gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt
     3841 acgctacgac cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga
     3901 ccgttacgcc catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct
     3961 ggatcgcgaa ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga
     4021 tcagatcgcc acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat
     4081 cgacgatcac cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc
     4141 cctcggcgac aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct
     4201 tgcccgctgc gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac
     4261 ctaccagcgt gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc
     4321 ttacaccatg ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt
     4381 agaaagcctg ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga
     4441 caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc
     4501 gccaacctga actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc
     4561 cgtgacccgg acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca
     4621 caagcagcga tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc
     4681 caaccggtgc tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc
     4741 aatggcgtcg acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt
     4801 ctgccactgc cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt
     4861 caggggattg aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat
     4921 ccgtggacca tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat
     4981 aacttctacg gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg
     5041 aaaaacttcc tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg
     5101 tgcgtctcca tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc
     5161 gcttacagga ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg
     5221 caaaagatcc ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg
     5281 tcccggtgca ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag
     5341 ctggcgttgc gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag
     5401 gctggtttga acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg
     5461 agcaaggcaa caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg
     5521 aacaactggt ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct
     5581 cgcgcgacgg cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat
     5641 atccgcaagt ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc
     5701 tacgtggcac tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca
     5761 ccgtgaagga ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga
     5821 tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc
     5881 actggcaaaa cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc
     5941 ttccagcgat ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga
     6001 cattccgttt tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc
     6061 ctttggcgcg gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac
     6121 tcgcctggcc gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga
     6181 cgccgaaacc ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt
     6241 cactcaatgg gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat
     6301 cgtcctcaac atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa
     6361 aaaagtgcgt gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga
     6421 acacttcctg gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt
     6481 ccacaaacaa ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga
     6541 gatcaggata tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt
     6601 ctgatgccgg tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg
     6661 aacccggaag ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact
     6721 aaacagcgac tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt
     6781 gccccggact gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc
     6841 cacaaaggca ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa
     6901 ctggaacaac tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa
     6961 ctgtatgcca gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc
     7021 gcactggcag caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg
     7081 tatcacctgc tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac
     7141 ctgcataaac ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg
     7201 gagccattgc ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc
     7261 ggataagatg cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc
     7321 ccgtttctct taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc
     7381 cccaacacaa tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt
     7441 gcctttttcg gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca
     7501 tgaatatcaa gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga
     7561 tagtagtttg gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc
     7621 gcttcgctcc ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt
     7681 ggctttgtca cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc
     7741 agatctttct gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc
     7801 cgtcccagag caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca
     7861 acttgctgaa tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa
     7921 gaaacagtca atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc
     7981 tcttcctgat tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt
     8041 aacgtctcct gcaaatccga tactttcggt gcagcctgac catagataac attcatggca
     8101 ttgttatagc cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat
     8161 ttctccttcg ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg
     8221 ataattgtca tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc
     8281 gggtcatggt tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac
     8341 ggaaatttcc aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga
     8401 taaaaataat gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat
     8461 aacaagatgt tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt
     8521 aagctcttct gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag
     8581 atcacgttcc aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat
     8641 cacttcaacg cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa
     8701 gttatctgac ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg
     8761 acaattgccg agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta
     8821 tctttcggca gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata
     8881 cgcggatcga gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca
     8941 taactatcca gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt
     9001 ttaatcgctt ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg
     9061 ccttcctgta acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata
     9121 cgtgacggat ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa
     9181 ataatatttt cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt
     9241 gatttgatga ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct
     9301 acactggatg tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg
     9361 acataatcag catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta
     9421 tctgattgca aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc
     9481 atagcaacgc gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga
     9541 agcccgtttg acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct
     9601 cagaattaac ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt
     9661 ttaacgctac gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag
     9721 aatagctgat gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg
     9781 caaaaaaaag cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat
     9841 taatccagcc attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc
     9901 ccgaaatagt cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta
     9961 tcgtaatagg caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa
    10021 gcgacgacat cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc
    10081 agcagcaggt tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac
    10141 tgcgcacgga tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc
    10201 caattgtatt cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt
    10261 ttgcccagat acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac
    10321 tgagcttgcg gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag
    10381 atataacgtg caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg
    10441 ctctggctgg tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc
    10501 aggtagttac cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag
    10561 ctgctaagtt caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga
    10621 ttcaggccac ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca
    10681 ataccattgt gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg
    10741 catggctcgc cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca
    10801 taagcctctt tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg
    10861 ccggaaacac cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga
    10921 atggtgtcct ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat
    10981 ggtttcaggg aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg
    11041 cgacgaggcg tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc
    11101 ttgcctggat tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag
    11161 acggtataac cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg
    11221 actacgccga tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct
    11281 attaaccgtt atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg
    11341 ttacagcatg cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact
    11401 ttgaattttg gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata
    11461 aacgaactat taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac
    11521 tcgggattac taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc
    11581 atccctttat agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat
    11641 aactcactat attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt
    11701 gaattaaaat tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc
    11761 gaaaaaaaat aatacacaca aggataaaat atcgtcccaa cttgtaattt gtaatgttga
    11821 tatcttttat taaacttgca attgagatcg caacctgcaa tactgacaat gggtaaatgg
    11881 gtgataaata cctgtcacct tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa
    11941 tcgagaaaaa tacccaaagt attttattat tcctaagtgt aaagacagac agtatagcat
    12001 aaaaaacaac atcactgcca actttattaa ttgcttgttg caaatctaat gtggcaggac
    12061 ttataccatg tcgagcttgt tctgctgttt gaacttgtag atcaatttca ttccataggt
    12121 agtgaccaaa tatatttcct ccagcaggac taaaaggatc accagagatc caaaaactac
    12181 ggacatacca ataaatgcca ccaagaattg ttgccaagct aaaaataaga aatgtctgta
    12241 tgtttctctt tttcgctatc aatataacaa atatgatggc tccatatatg gcaccaaata
    12301 gttttacgcc acaagcaatt ccaataaaaa aaccacataa aaaaattaat tcattttgtg
    12361 ttttattgtt ttctgagcga atatatataa gaatactggc tgcagcaaaa caaagatagc
    12421 gttaaatcaa tatatgcata accaagatga tctttaagtg gattgatagt aaatagtagg
    12481 attgtagcaa taatgcctgg ataataagtt ttcgtaattt cccatgaaca gccaacaagt
    12541 ccacaggcta ttacaaaaaa acagatattt gctaaaaact gagctaacct tacatctcct
    12601 aactgtaaac ctaaagccat taaaagatct gcattttgcg ggaacagagg aaaacgcaaa
    12661 tattgttcaa caacaattgc atggtgctca actaatgaac gagccaaagg taagtgatac
    12721 atagtatcat cccaatcacc gggagctcgc agggacgata acaatgtaac cacaaaaatc
    12781 aaaacaagaa ataaaataac aaacagcgta ctgaactcaa tattatttat tgccagccat
    12841 ctaaattgct gacgtttaat aatgcttaaa acccaacaca ccgtaaaaat taaaaagata
    12901 gcaatcttat taaattgccc gaccatgccc agaaagtaaa ttatcaaaat atttaaagac
    12961 atccctatgc ataggcaata aaaaataaag aaaatttgtt aagtcctgcc accccctcct
    13021 taaaaaaaga tgacaataca atattaccaa tgacaaaaaa tgtcattcca aaagcaaaac
    13081 aatacacaac atacaaatac aacggcaatt caatcatagt aaaatactct atttacatta
    13141 tcagtttata gtttttttca gaaagataaa ataatgcctt catttcacgt ctagttgttg
    13201 tatttgaatg caaaataatg ccacatacaa atgccattat tgacgataac atgatggagg
    13261 cagttaaaaa ggcagtcgga aatcttctca ccaaacctgt atcaatataa tcaacaatga
    13321 tgggcaatcc taaaattata gacaaagcag caagtaaaac gccgataata ctaaaaaagt
    13381 atagtggccg ttcaagcgag taaagtttaa ttattgtttt cagaatcttg aacccatcac
    13441 tccaagttga tagtttgctc acactccctt cagatcgctc gccatatttt gtattaacct
    13501 caccatactt catacgtaat tctaaagcat gaattgtaag ctcagtttcg atttcaaatc
    13561 cccttgatag acatggaaaa gatttaacat agcgtctgga aaatacacga taccccgaca
    13621 acatatcagt aaaattccct ttaaagatag tctgaacagt accagtcaac aatttatttc
    13681 ctcagcgatg cccttttctg tatgtatttt gatctccatt ctcttgacga catcctacaa
    13741 ccatatccaa ttgatttttt atgagatggt taatcatttc tggagcagaa cttgcatcat
    13801 aagtgtcatc tccatcaacc attagataaa tatccgcatc tacatctgag aacatacgtc
    13861 ttacgacatt ccccttgcct ttcagaggaa cagagtgaac tgttgctcca gcatcctctg
    13921 ccacttgtgc tgtactgtca gtcgaattat tatcaaatac atatatagaa gcagttggaa
    13981 tattcgtttt gaaatcattt atgacttttg ctattgcctt ctgctcatta taacaaggaa
    14041 taattacagc gatattaaaa gagtgataca taagcccctc atataaacta tttagctaat
    14101 gtgcaagtaa ttgtgttttt aacaaatcgt ttactaacga aaaatgaaat attttgatgc
    14161 gcaatatcct aaaaccaaac taaaaagaga aaaggtgaac aatgtaaata ttggagataa
    14221 agctaagaga tcaaataaaa cgccgaataa ataactcatc gcccccataa aaaatataaa
    14281 tataaaatat ctagccttgg ttgcagatac tttaaatgag catcttgcat ttgcaaaaaa
    14341 agaaaaggtt gctgcacata taaaaccaac aatgttactt cggccttgac caaaagctaa
    14401 attattataa cacgcataaa atacccccca atgtaaagca gtattgacaa ttccaattaa
    14461 aaaataacta aaaaaagaac tggtcaataa cttccctatt tttaacatcc tttattttgc
    14521 tccagaagtg aggttattac taatttggat attttctata gaaaataccc ataaaactac
    14581 aacgcctata ataaaaattc ccatgggtgt aaaaagaaat ggattagtta tagaaacggt
    14641 aatactgaaa aaaagtatta acgacatcat taatgatgtt tttgtactat ttaaaaaata
    14701 gcctttcaaa aagataagca acataggaac taatgatata aatacaccaa taacgcctgt
    14761 tttctgaagt atctcaagag gtactatttc aatccttaat cgatctaata taaatgaacc
    14821 aaatcctctt ccaaacaaga acgtcgctaa atcaacattt ttataataaa aatataaatc
    14881 attaaatcta acagagtcag aatcttcact gcgtgatccg accatgtaaa gcataattat
    14941 aaatagaacg ctgcccaata ttattataat agtatttaaa tatagcttat gtaatttaaa
    15001 actggctaat aacagtgata atgttgtaaa aagtaataca cctcttgatt ctgtcaataa
    15061 tatcgcaagc actattatga gacattttaa atattttttg tcgacaaaac tgataatcgc
    15121 accgacacca aaaaagtaga aggatttgga aaaaaaagcc ccatcgggac gaaaaaagaa
    15181 ctcgctagtc aatgataagt gttcataaat taaattaaaa tttaacaaac cgaaattcaa
    15241 caaaattatg tatgaaatat ataacagtga tccatataat gcaaatgcat taactatttt
    15301 aataaaaatc tcacatgaaa atacttttaa agtatatctt agtgtaaata gatatataag
    15361 tgacagcata aaaaggtatg gttgcacatc aaacagtata tcatttattt tattaccagc
    15421 taataagccg acaaagaagc cataaaaagg aaataaaagc aaccatataa aaagactgac
    15481 acatttttgg gtgattgaaa aattgaatgt aactaaatta aaaattaaaa gtataaaact
    15541 aaatacaaat aataaatatc gcaaagggaa tattcccaat ggctccagta atcttccacc
    15601 accaccaata atcagttcaa tacataaaaa tgttataaaa attttattta tattattcat
    15661 attcgtaagg tgatgttttt taaaatatgt ttaaaatcaa cattaataaa atataaattt
    15721 tatcagcctt tttccgcctt aacttccaaa aacttaaagc caagatcaaa ttgcctatat
    15781 aacctttacc caattgatta ttcacaaagc tatcatcagc taaggcatta gctattttag
    15841 ttacttcttt tcgataccac ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa
    15901 aactaatgtt tgctcccact tggttgtttt catgctgtct gtacagcatt gttggttttc
    15961 tatcaatata ccacgaataa tttctcgtcc gcgcaaatga atataaaaac caatcatgca
    16021 gacagatatc ttgtgcagca tttttattat ttatgatgaa cttcttaaat tcaataagcg
    16081 tttctttttt tataacatat gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt
    16141 gagtttgcga ctgagcctta tcaactaaat tttttcgccc tgatggataa tatgcaatta
    16201 cattgctgga ataacaattc gcattattaa ataccaagca gtcaattgca ttttttattt
    16261 tatctttata ccaaatatcg tcctgatcac taaaacagat ataatcatag ttttcaagat
    16321 cgacatcttt aattaatcta taaaaatttt tacctgcccc cccaaactta ttgacgcttg
    16381 gaagtatatt tataattgag ggatatgata attgaaattc ttcacaaata tttaatgtat
    16441 tatcagttga taaatcatct gatataaaaa ttgagatgtc tacatctttt tgttcaataa
    16501 tactttttaa ttgttcctca atccagcatt cgccattata cgtagccata agtacagcta
    16561 ctcttttttg atcagaacta tgcattataa cgaccgcccc cagtaattcc tcttattcca
    16621 tccagcgtag ccaaaatatg ttttttcata tattcaattt tatttttttg agttgcgata
    16681 attaaagtcg tatgaattaa agaaaaaaca acttccctaa tagagagtaa aagaggcaca
    16741 tgattttttc taagcaaaag aaatgcattt cttactcgat aataacgacg tactggcgag
    16801 tgaattggta tgttaatccc acagattttt actgacttat ccccaatagc atgaaccatt
    16861 tctatatcag gaatgatatg aactaatatg ccatagttta agcatctaag acaccattct
    16921 gtatcgacat aatcaataaa aagttcctca tccatcattc caacaatctc aaaaacttct
    16981 tttgaaacca tggttccgga tgagattgtt actgaacttt taaacggtgt ctgtccttca
    17041 gtgacatgaa ttttctctcg aagaccattt tttttgatat tacaaatagg ataataatgt
    17101 gacttatctc tatcaataaa aactgggcca gctatttttg cattttcatt atccatatag
    17161 caggaaagtt ttttgataaa ctcattgcga atacggctat cttgatcaaa aaaaattatc
    17221 ttctctgccc ctttttcaag agaatatcga atcccaatat tttgtgcttt tgctatacct
    17281 tcattcctac caagacaaat taaatgaact ttttcaatgt ctgctaattt atctttcaag
    17341 gtgaaattag tcctattatc gactactaca acgtaacaat tttgctcctt tagttcagtg
    17401 atcagtgcat tcagattttt aagttcggga ttatatgtca caatgacagc gtaaatatta
    17461 ctattcatta tccaagtgac tcagtaattg gttaatttta gttttcatat acagaacaca
    17521 ttctagtata aagccattgt aataacagag catctaaaat tacacgtatg ctccaggata
    17581 ttgcggcacc cagtaaccca tatttttcga ccataaaata caaaagggct aaataaggaa
    17641 ccaattcagc acaatgaatc aaagcagtaa ttttagattt tcctaatgcc tggattgcag
    17701 agaatggtat cattgccagc gcattgaaga aaaaaccgat caataacaca tttaatatat
    17761 taaaggaatt aatttgataa ttctcaccaa accatatttt caatacaagc cctgaatata
    17821 acaagcctat gattatcacc ggcaaacaga ttagaaacat taaaagcaat gatttattta
    17881 cattacgttt aaattcttta aagtctttga tgttacttaa ccttggaaac actgccctcc
    17941 cgattgctgc aggtattatt cctaatttta gaataacctc cgctggtgca gaataaaatg
    18001 caactttatc tgcccccata atatttgaca ctataaatcg atcaaaatac accatgaccg
    18061 gacttatgat attacttact gtcatccaac cgccaaagaa aaagagtcgt ttaaaggttt
    18121 tataacaaaa atgaaccccc gaaattttaa tatcattcct gacataatag gcagagacta
    18181 aaatcgaaat aactcttgca aatattaaac cagcaaccgc tgccgacaat gtagcactat
    18241 aaaaaacaaa tatggccgga attcccgcga tgcaagagct tgatatagat ttttgaatat
    18301 ttacaatgcc aaatttttca tccccctcaa gaatggctga ccataattga tttagaataa
    18361 ataatggaat gcaaatagcc aacagtttaa atgctagttg tatatcacta tgttcaacac
    18421 cagaaatttt tagataatta acaattccat cagagaaaat caataataaa aaagcaccaa
    18481 agcatgaaaa tagcaccaaa aaagatgtac ttgttgaaat taccttggtt ctttcatgat
    18541 gattatcacg atgaattgca atttctctaa taacagagcg agtcaggcct acatcaaaaa
    18601 ttccagcata cccaacaagt gcaattgcaa ttgtataaac accgaaacct tcaggcccca
    18661 aacctcgagc gagaaatcct agcgagggga tagcaattaa agttggtatt gcatagccaa
    18721 aaaggttcca gacactattt tttattatgc tcattattac atttctttat catataaaat
    18781 aatcagcatc tacaaatttt tgaccattaa tatctttatc agaaagcaat ggattttgaa
    18841 ccggccattc tatatttata cttttgtcgc tccataaaat cccacgctct tgcatttttg
    18901 aatagaaatt atttgttttg tagacaaatt ctgcttcatc actaagaacg caaaacccat
    18961 gagcgaatcc ttccggtatc cataattgac gcttattctc cgccgacaaa tttacaccaa
    19021 cccacttgcc aaatgttggt gatgatttcc gaatatcaac agctacgtca aaaacttcac
    19081 cgacaacaca gcgtaccagt tttccttgag cataaggttc cagctgatag tgcagtccgc
    19141 gtaacacacc cttagttgat ttagaatggt tatcctgaac aaactcaacc tttcgtccta
    19201 cagcctcttc gaaaactttc tgattgaagc tttccataaa aaagccccgc tcatcaccaa
    19261 atacttttgg ttcaaaaatt agcacatcag gaatttcagt tttaattacg ttcattttat
    19321 taataacctt taatcatttt cagcagatac tgaccataag catttttctt cagcggctcg
    19381 gctaatacct tcacttgctc agcatcaata aagcctttac ggtgagcaat ttcttccggg
    19441 caggaaactt tcagcccctg acgctcttca attgttgcaa tgaagttgct tgcttcaata
    19501 agactttgat gtgtccccgt gtccagccat gcataaccac gccccatcat agcgacagac
    19561 aaacgtcctt ggtccatata aatacggtta atatcggtaa tttccagttc gccacgtgca
    19621 gatggtttaa ggtttttcgc catttctaca acgtcattgt catagaaata aagcccagta
    19681 accgcatagt tactttttgg ttcctgcggt ttttcttcca gactaattgc cgtaccgtta
    19741 tcatcaaatt ccaccacacc gtagcgctca ggatcattta cgtgataggc aaaaaccgtt
    19801 gcgccactct ctctgttaac agcggtatcc attaatttcg gcaggtcgtg accatagaag
    19861 atattatcac caagtaccag cgcacaatca tcaccaccta taaattcttc accaatgata
    19921 aatgcttgcg ccagaccatc cggactcggt tgtactttat attgcagatt aagcccccac
    19981 tggcttccat caccaagcaa ttgttgaaaa cgtggtgtat cctgcggcgt actgataatc
    20041 agaatatctc gaatacccgc caacataagc gttgaaagcg gataataaat catcggttta
    20101 tcataaatcg gtagcagttg tttgctcaca gccattgtca caggataaag acgagtgccg
    20161 gaaccaccag ccagaataat acctttacgc gttttcattt caccattcct tttaattcat
    20221 ctcgctctag catcatgagc gagatgcaat aatttgttaa attgccgtgg tcgtaaataa
    20281 ttcgttgagc atacgtttca cgcccacctg ccagtcaggc aagacaagcg caaagttctg
    20341 ctgaaatttt tcggtattga ggcgagaatt atggggacga cgtgctggtg tgggataggc
    20401 cgttgtcggc acggcattaa gtttgttaag agcaaggttg atccccgctc tgcgcgcttc
    20461 ttcaaatacc agagcggcat aatcgtgcca ggttgttgta ccaccagcaa ccagatggta
    20521 caaaccagca acttctggtt tatttgcggc cacgcgaatg gcatgagcgg tgcaatcagc
    20581 cagtaattca gcccccgttg gtgcgccgaa ttgatcgttt attacagcca actcttcgcg
    20641 ttcttttgcc aggcgcaaca tcgttttggc aaagttattt cctttaccag cgtataccca
    20701 gctggtacgg aaaataagat gctttgcgca atgttcttgt aacgcttttt ccccagctag
    20761 cttggtttca ccgtagacat ttagcggtgc tgttgcatcc gtttccagcc atggcgtgtc
    20821 accgttgcct gggaaaacat aatcagtgga gtagtgtata acccaggctc cgacttcatt
    20881 agctgctttt gcaatcgctt cgacgcatgt cgcgttaagt aattgcgcga aattcggttc
    20941 tgattccgct ttgtctactg cagtgtgagc agcagcatta acaataacat cggggcgaat
    21001 ttttttgacg gtttcagcca caccttcggg gttgctgaaa tctccacaat aatcagtgga
    21061 gtgaacatcg agcgcaatca agttacccag aggagccaga gcacgctgca gttcccaacc
    21121 cacctgccct gttttgccga aaagcaggat attcattact gacggccctc atagttttgt
    21181 tcaatccacg actgataagt accgcttttc acattctcaa cccaatttgt attagccaga
    21241 taccattcca ccgttttacg aatcccgctc tcaaacgttt cttgcggttt ccagcccaat
    21301 tcgcggctaa ttttatcggc atcaatggcg taacggcggt catgccccgg gcgatcggca
    21361 acataagtaa tttgctcacg ataagatttc tctttcggta caatctcatc caacaaatca
    21421 caaatagtga gcactacgtc gatgtttttc ttttcgttat gtccaccaat gttataagtt
    21481 tcacccgctt taccttcggt tacgacggta tataacgcac gagcatgatc ctctacatac
    21541 aaccagtcgc gaatttgatc ccctttacca taaataggta atgccttacc ttccagagca
    21601 ttcagaataa ccaatggaat aagtttttcc gggaagtgat aaggaccata attgttcgag
    21661 caattagtca caatggttgg taaaccataa gtccgcttcc aggcacgtac caaatgatcg
    21721 ctggaagctt tagacgcaga atacgggcta cttggcgcgt aagctgtcgt ttccgtaaat
    21781 agcggtaacg cttcgttatt atttgcttca tccggatggg gtaagtcacc ataaacttca
    21841 tcagtagaaa tatgatgaaa gcggaaactt tttttctttt catcattcag agcagaccag
    21901 taattgcgcg ccgcttctaa aagaacataa gtccccacaa tattggtttc aataaatgcc
    21961 gcagggcccg ttattgaacg gtcaacgtgg ctctctgccg ccaggtgcat caccgcgtct
    22021 ggctggtgct gtgcgaaaat acgcgacatg gctacagcat cgcagatatc tgcatgctca
    22081 aaagcataac gttcagaatc agaaacatca gcgagtgatt ccaggttccc ggcgtatgtt
    22141 aatttatcga cattaacaac actatcttgc gtattattta ttatgtgacg aacaacagca
    22201 gaaccaataa accctgcgcc accagtaaca agtatcttca cttttttatt ccatatagct
    22261 atagagcatg ctgtgaaata gactgctcca gatttgatta atagaggtat taatacacgc
    22321 taccgcccct ggcttaacag ctaccagtgc actgcatgca tctcaacgat gtggcgagcg
    22381 ttagccactc gcgccaaatc cgaaaaattc aaacgttaat tgtcttacca aacagccgta
    22441 gaaacaagga aaatcctgga aaaatttgaa taatatgagc tatgtaactt cttgttttta
    22501 taggctttat aaaaacggca acggcaggaa aattcaatga gcaaaactta tgccacgaat
    22561 cttcactgcc gttataattt tcttatcaac cgttacatcc ggtcagattt tcattattcg
    22621 cttaacagct tctcaatacc tttacggaac ttcgcccctt ctttcaggtt gcgtaggcca
    22681 tacttcacaa acgcctgcat atagcccatt tttttgccgc agtcgtaact gtcgccggtc
    22741 atcagcattg catcaacgga ttgttttttc gccagctcgg caatagcatc agtcagctga
    22801 atacgtcccc atgcaccagg ctgagtacgt tccagttccg gccaaatatc ggcagaaagc
    22861 acatagcgac ctacggccat gatgtctgag tccagcgtct gcggctgatc cggtttttcg
    22921 ataaattcaa caatgcggct gactttaccc tcacggtcca gcggctcttt agtctggatg
    22981 acggagtatt cagagaggtc acccggcata cgttttgcca gcacctggct gcggcccgtt
    23041 tcgttgaaac gtgcaatcat ggcagcaagg ttgtaacgta gcgggtcggc gctggcatcg
    23101 tcgatcacaa cgtctggcag taccacgaca aatgggttgt caccaatggc aggtcgcgca
    23161 cacaaaatgg agtggcctaa acctaaaggt tcgccctgac gcacgttcat aatgatcacg
    23221 cccggcggac agatggactg tacttccgcc agcagttgac gcttcacgcg ctgctcaagg
    23281 agtgattcta actcataaga ggtgtcgaag tggttttcga ccgcgttctt ggacgcgtga
    23341 gttaccagga ggatttcttt gatccctgca gccacaatct cgtcaacaat gtactgaatc
    23401 attggcttgt cgacgattgg tagcatctct ttgggtatcg ccttagtggc aggcaacata
    23461 tgcatcccga gacccgctac aggaataact gcttttaaat tcgtcattat ttcatccacc
    23521 tgtaaaatgg ttgctgaatt atagcttgtt cgattttttt cgccagcatc aattaccctg
    23581 aattgattac tgaattactt gtgatgttac gccgcttcgt tgtggattgc agtagcattg
    23641 ttcctaagta tggctccatt tttccaggaa tggtcgcaaa tctactccct ccgttccggc
    23701 aatctaaagt taatcttttc cacattaaca atatggtgat taatcctgtc gatatcgacg
    23761 gagctttgcc ctctttcatt caccgcatga acatttgcaa gagacagcag tgtttctttt
    23821 ttcgccataa agacgccgcg aacgtctttg cgcatgtcga agttcatgct caatgctggt
    23881 ccaactgagg attcctgcat cacattgata tttcgcataa aaagatgttg cggcttgttg
    23941 tgtaactcca gcgaggcacg cttcatctca atgttagtta gcgacacaaa ggagacggca
    24001 ttcccggcgg agatttggat gccgcgcaat ttataagcaa gatgggtatt atccagttga
    24061 atattattca ctcggaaatt ttgcggtatc gagagatatt tgcctttaat taccccatag
    24121 ccgattaaca tcccggcgct attaatcatt tcaatattat caatcacgaa attgtcacaa
    24181 ccgtaaatag cgactgtcgc gttatcaatg cccgctttct tactgaaatc cggcgtgata
    24241 ttgcgggcat tgatattacg aataacaaaa tgtttaccat tttcaacatg gatcaactgc
    24301 cgacaatccg atcccgtgat attcgccacg acaaagtttt tcactgcctg gtcttccggg
    24361 tagttgttat cataagtgct tcccgcaagg cctatgccga tgccccagtt gattttgccg
    24421 ttggtacagt tgatacgctc gatgacatgg tcagatatca aaatatcact gtcgttaatt
    24481 gccacgttcc attcaatggc gtcgccctgt aagtcgctga acttacagtt ggtaatgttg
    24541 gcaccgataa tctggttatg aaatccctgg cgtaagatgg cgtaattagc gtggctgaca
    24601 gtgaggttat cgatggtcag gttgcgcatg acccgtttgt ttttgccgcc aatataaatc
    24661 tgcattaccg ggccaaagcc gctcatcgcc agccctttga tggtgcagtc agaaccacgc
    24721 acatccaggg taatgttatg cataccgccg ccctctcccc ctgtcacctg gctgccgtcc
    24781 tgtaagacaa atcgccctct gccgttgccg cgcaggcttc caaggatgtg taacgttttg
    24841 ccgggaggga tgaagatgcc ggtgttgata ttgtcacaaa cgaatccggc aggcacgacg
    24901 accgtttgcc cttcgctgaa ggcttgttta aatgaggcga tccagtcatg tgggttgtag
    24961 tcgttaatgt taacgctttg tcgggcggga agtgcgcgag cgaaaggggt atggaggaag
    25021 gcaagcgccg agcttgccgt caggaaggtg cgtcgggaga gtgttttaaa tggcatacgt
    25081 tctcctctat aaagcctgca gcaagctggc gagttctcga ttaatcacct gctggttaaa
    25141 atcgtgttcg actttttcgc acgcacgttt gacgaccgga gccagttcgt cggtatccag
    25201 ttggctaaac gccgccagac gttgtgccag tgcgcgagca tcgttctcag gcagcagcca
    25261 gccggattta tcggcctcca ccagttcagg aattccgcta tgcagagtag aaaccaccgg
    25321 aataccgacc gccatcgctt ccattagcgc caccggaatg ccttccatat cgccatccgc
    25381 gcccgttacc gatggcaaca ggaagacatc cgcgtcgtcg agcatctcct tcacttcatg
    25441 gctcggttta aagcccggca tctctaccac atcttccagt tgatattgtt cgatgatggt
    25501 gcgcaggcgt cgttcccaca ggccaatgcc gaggatgcga tagcgaaatg ccacgccctg
    25561 ctctttcaac tgacggcagg cttcaatcgc cacatgcagg ccttttttct cggttaagcg
    25621 tgcgacagat atgatttcca gcggcgttgc gggcgctttc accggacgcg ggctaaagcg
    25681 cgtcatgtcc acgcccatac gcgatacggc gattttttct ctggggcagc ccattttttg
    25741 cagccttccg gcccacagat cgcttatcgg taacatcagg tcgccacggc gaaacagttg
    25801 ttgatattcg ggagtgtagt ggttgagcac ttcccgactg gagatatcaa taccgtggaa
    25861 gatggtggca attttgccgc gaatgatacc cagttcgcgt agttttgctg cggttacccc
    25921 cgccgggcca aaatgagcga tgaacacatc ggcgcgaaac ggtgttgcga cctgaccaca
    25981 aatggcagac aaaatcaggt tccgcgactc ggcaccatag cgtttgaggt tgagcgcatg
    26041 ccaggtattt ttacgatgaa tgccacgtaa ggtctggctg gcgcggtggc gcagtttcgc
    26101 tactttgccc gcaggttcgt cctgtaacca gcgggttctg gcgacaaggt tgtatttcgt
    26161 ccatgccgcg tgggtgttct ctgtatcgcc tttttgcaaa gcgacaatct ccacttcaaa
    26221 gcccatatca ataaacgcgg taatttgatt gaggacgaag gtttccgacg acagcgggaa
    26281 tttcaataag aagaagccga ccttcatttc acctccccga tgcgctcaag cacagactgc
    26341 accatctgca ttcccgtctg acgttcgcga ctgacggctt cgttaaatcg agcgttcagc
    26401 accggaagct ggcctaaggt atccgcaacc atcgcttgca ggctgccgtc cagtaaatga
    26461 cgaatatcaa tcgccatctc tggcagtccc agctgctgca taatcccggc ggatttatgt
    26521 tcatagttga tggcaatcgc cggagtagca aagttcattg agataatggc ggagtgcagg
    26581 cgcgtaccga cggtgagttc acaggctccg agaattttgc ccatttccag atcgttgagt
    26641 tcatccatca ctacgtggta acgggcagga tcgctgatgt gctggcgcag attgagcgcc
    26701 accatgcggt cgtctttgtt atagctgtcg atacccgtac aggtggagag cgcaatcacc
    26761 tgatagcctt catcgagaat gcgattgacc accccggcaa aggctttttc atacgcttgt
    26821 tgagtggtgc cgagacgttt gtcgaacggt gccagttcgc gcagggtaat tgccactgtt
    26881 ttctgttgtg cggcaacgtc cagccagtgt tgaacggcat agctggcggt gaagtcttcg
    26941 gtgtggtgat cgaccagcca cgcggtatcg acgccatgtt ccacttttgc ggtggtgata
    27001 tttctgcgtt tcatcaaatc aaggctgacc gattcgcgca ggatcagcgc gtcgcagtga
    27061 ccaaagacat agttcgccag ttggttaaat tgctcatcct ggaaggggcc gacgctgtga
    27121 ccaatcataa acagcggctt tttcgccata aatgtgcaaa gcgcatgttc aaactgcggc
    27181 acgccgtaga gatcgacaaa aaacgatccg ccgacctgaa taatggcgtc gtaacctgac
    27241 agcaggcgca cgaagtcggt gaatccctgg gcgatggcga tattgcgcag cttgccagtg
    27301 tcggtgacgc gtgagagcaa tacctgatgc tggtagcggc gacggaggac ttttttaatg
    27361 cgcccgacaa cgcccgccgc gctattgtgt tgtgtcatct gcaggaacag cggatcgccc
    27421 attaccgggc ggttgagcag ccaggaagaa ctgaccggat agcggctcat cacgtccact
    27481 tcggtatgtg gattgaggat gttgatggca tcaagtaagc cgcgcaagat ggcgctgtcg
    27541 ccacgattgc cgcaagtgtg gttgcccaga ataagtaatt tcattttttc ctcataaatt
    27601 tgatgccagg tgaggctgtg tttgttgtgt tcgttgccgt tttttgcctg atgcgacgct
    27661 ggcgcgtctt atcaggccta caggtcccga gcacagaacc gtaggacgga taaggcgttt
    27721 tacgccgcat ccggcaaccg ttgtcggaac cgaaaacagc aattcacccc gcccgtaaaa
    27781 gcattttcat tttttcgctg cgacaaaact gacgcttcac ttccaccacc agcggatggc
    27841 gtgacagcac aatcatcacc acaaacgcca gcacgcccgc ggctatttgc accgccagca
    27901 gcatccccag cgccagttgc cctttcagca caatgcccag cgcataactg accaccagcg
    27961 tcggcaacga gagataaaac ggcagccaca aactcaggat gtactggcga taactggaac
    28021 caagaaccgg tttaatcatc acgaagtaac tcagaatggt gttgataatc tgcaccagca
    28081 ggaaaccgag cgtgacgccg attgcgcccg ccatctgccc accaataaca atcgccggaa
    28141 taaacagaaa tgttttgaat acgttgaatt taaagctgat atcgacccgc gctttcgcca
    28201 tcagcagcga accaatcggg ttacccacgg agcgcagcag acccacaata cacagcaatt
    28261 gcagcaccgg aataatgctg ttccactttt cgccgaatac cagcggtacg aagttattcg
    28321 acaccaccat cagccccagc agcgccggaa agttgataat ccccactatc gacagcagct
    28381 tgtagaagtt aacacgcagc ttttcggtgt cgtcctgaat tttggcaaac gccggaaaca
    28441 acacgcgggt gatgattggg ttcagcttca tcggcggtac gacggccacg ttgtacgcca
    28501 ggttgtatcc ccctgccacg cccgcgccaa gaatacgcgc cagcaccagc gttgaaaggt
    28561 tggtgttgag atagttgatg atgctgtccg ccgtcagcca ggcaccaaaa cgtaagttcg
    28621 gtgccacaga cgccagcgag aagtgcagac cggggcaata aattttgcgt ccaaagtagc
    28681 caaacagcag tgttctcacc gcactattga ccagataacc gaggatcgcg gtcatcgcca
    28741 gcggccagaa atgggcgcta actaccgtaa aagtgaagcc cgccagcacc gcgctggttt
    28801 cgatcatgcc gattttgttg aactccagct ccttctgcat caacgcgcgg aactgttgcc
    28861 cgtgggggat caccacaaac gccagcgaca atgttttaat caacggtgcc aggtccgggt
    28921 tattcagcac gtcgccgatg acatcactca acaaaaacac cgccacacat accacaagcc
    28981 ccagcccgac gttcagccag tacagcgtgg tgagttcaag gtgactgatt tcttttcgct
    29041 gaataatcga gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg ataatcacca
    29101 gcgacacagt cagcaggccg aactggtggt tgtcgataat ccgcgccagc acggtcatct
    29161 gcaccagccc gaggccgatg atgatcaccg tggcaatagc cgaccacttc gcgccgctga
    29221 tggttttttc acgtaagctc atatcaatat gccgctttgt taacgaagcc cttgaatacc
    29281 gtcaggaaaa cgattttgat atcgaaccag acgctccatt cgcggatgta ctcaaggtcg
    29341 aactcgacgc gtttttccat tttctccagc gtgtcggttt cgccgcgcca gccattaatc
    29401 tgcgcccagc cggtaatgcc cggtttcact ttatggcgca gcatgtagcc ttcaatgagc
    29461 tggcgatact gttcgttatg cgctactgcg tgcggacgtg gaccgacaat cgacatcccc
    29521 ccggtcagca cattgataaa ctgcggcaat tcgtccagcg aggtgcggcg cagaaagttc
    29581 cccactttag tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt gtcgttctcc
    29641 atcactttca tggaacggaa cttccacact ttgatcggct tgccatccat accatagcga
    29701 gtctggcgga aaataaccgg ccctggtgaa ctgagtttca ccgccagcgc aatacagcac
    29761 agcaccgggg agatcagcag cagaataagc gtcgccagca caatgtcttc cgcacgtttt
    29821 agcaggcggt taacccccga aagcggcgtg tcatacagcg gcaccaccgg aacgccgttc
    29881 atctcttcga gacgtgaatg gagaatgttg aaggtaaaga cgtcggggat cagcagcacc
    29941 gaacaggtgg tatccgccag ttgatggacc agttttttca ctcgtgcgcc gtcgcacatt
    30001 tgcatcgcga tatagacgtt atgaatcttg cccgcttttg catcctcgac cagctgttgc
    30061 aggttacccg cccagtcgtt agaaacgccg cccggtttcg ggtcgtggta aacgcccacc
    30121 acttcaaacc ctaaccacgg ctgattacgg aaactctcca tcagcatttg tccggcggct
    30181 aaatcccccg ctacggcgac catccgcttg ttgtagccat gattacgcag ccagcccgcc
    30241 ccaatgcgaa tacatgaacg gcaaaccacc agtccgatgc tggtcagccc ataccacgcc
    30301 agccagattt tcagttgcgt gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa
    30361 atcacgctta aggtccagtt ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg
    30421 cgccatgagc gataaaaatc ggtgatgccg cccagcatct ggaacaccac cagcgtaatc
    30481 agcgccacca acaggtgcat gtagaggaat gacagtccgc tgacttcgca aaccagccat
    30541 agcccggcaa acatgatggt gatatctgaa aagcgttgca ccatagagat taacgatgca
    30601 ttggttttcg ctcgctcgcg cttttttaga tttgtcatcg ttgttcctgt tattagcccc
    30661 ttacccgcag tggggtaagg gaagatccga cattactcgt tcagcaacgt cagcagagtt
    30721 cgcgttcgcg cttccatcag cggcacatca ccgcgcgatt ccacattcag gcgcaccacc
    30781 ggttcggtat tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca
    30841 tcgctgcgat ccaccgccag cgcctcacgg ctaaaatgct gttccacgcg gttaatcgcc
    30901 tcaacgggtt gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg
    30961 tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc
    31021 cacgggatca tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt
    31081 tcgccaccgt agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt
    31141 ttcgacatta ccggcgtgcc gcctgcagca gtcaccacat caatagtgtt ccaagagaga
    31201 cgcggatcat ggatgatctt cgcgccggga tttttttcga ggaacgcttc tgccagcagg
    31261 ccgacaatgt agtagccctc gataaactgc cctttttcgt caaacagaaa acagcggtca
    31321 aaatcgccat caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg
    31381 tcgtcgcgac attccggcag cagcgggtta ggaataccgt tggggaaatt gccgtccggt
    31441 gtgttgtgta ctttgattaa ttccacgggc gcgccgaggg ctttaaagcg ggcttcaatg
    31501 gcgtccacca ccggacccgc tgcgccattc ccggagttga tcaccagctt gagcggcgtg
    31561 agatttttga cattgatata accgaacagg tgatcaacgt aagcgtcacg caggttgatt
    31621 tgctgatagc gaccgcgttt ggtttcatcg acgggaggaa agtcgttggc ttcagccaga
    31681 cgctggacgt cgcgcagtcc ggtatcaccg ctgatcgtgc gagccccctc gcgcaccagc
    31741 ttcatgccgt tgtaatccat cggattatgg ctggcagtaa cttcaatgcc gccatccacg
    31801 ccgagatgga acgtggcgaa atagatctct tcggtgccgg acataccgat atccagcaca
    31861 tcgacgcccg catcctgtaa accttttgcc agcgccagtt ttaaggtttc gctggtgaga
    31921 cggacatcac cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca
    31981 atgcgccagg cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct
    32041 ttaaagcagg ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc
    32101 gaaacggcca attttatttt tgtcgttatt tacaccgcgg tttcgcattc attgcctgat
    32161 gcgacgctgc cgcgtcttat caggcctaca aaaccgaacc gtaggtcgga taaggcgttc
    32221 acgccgcatc cgacaaacag cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg
    32281 aagcgcacca catcatcctc ttcgagataa gagccggagc gcacttcaat taaatcgagc
    32341 ggaattttcc ccgggttttc caggcagtgc gtcgccccca gcggaatata aatggactcg
    32401 ttttcaccaa gcagtttgat atcaccatca atggtgactt ttgccgttcc cgcgaccact
    32461 acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg
    32521 gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag agtcatattt gccccacgga
    32581 cgatacactt cgcgatgcac ccgatgctca tggcgaccat cggctttgat ctgctcgacc
    32641 acttttttca catcctgtac cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc
    32701 accactacca gatctttcac cccgacggtg gtgaccaggc ctgattcggc atacacatag
    32761 ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg
    32821 gcgctgatct cccataatga agaccaggaa ccaacatcgc tccagcccgc atccatcggc
    32881 accacaacag catctgccgt aggttccatg accgcgtaat ccaccgactc ttccgggcag
    32941 gcgagaaacg cttcttcatc cacacgaata aaatcgagat ccggatcgac ggcgctcatc
    33001 gctttttcgc aggcatcgag gatatccggg cgatattttt tcagttcttc gagatagcgt
    33061 ccggcgcgga acaggaacat gccgctgttc cagtaatatt cgccgcttgc tacataggcc
    33121 tgggcggttt ccagattcgg tttttcgaca aactgcgcca cttcaaaggc caccgtatcc
    33181 tgctcaccca cagacacttc accgcgacga atatagccat aaccggtttc tggtagatcc
    33241 ggcacaatgc caaaggtcac cagcttgccc gcttcggcat aaggcatggc attacgcacg
    33301 gcggcacgga acgcgtcttc atcggcaatc acatgatccg ccgccaatac cagcattaac
    33361 gggtcgctct ccgggctatg acgttttgcc gccagcgccg ccagtgcaat ggcaggtgca
    33421 gtgttacgcc ctgccggttc gagaataatg ttctcggtga gtttgttcag ttgacgcagc
    33481 tgttccgcga caataaagcg gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc
    33541 acgccgttca ggcggcagat ggtggtttgc agcatggtga gatcgccttt caggcataaa
    33601 aactgcttgg ggtaaagtac gcgggaaagc ggccataaac ggctaccgga gccacctgcc
    33661 atcacaactg gatagagttt cgactgcgcc ataattatcc ccgaatatca tttataaatt
    33721 gacgtaacac gttctcttta tcgagcgtgc gttcggcata ttcacgtgcc accgtgttat
    33781 gtttgggcag caggagcgcc tgacgaatcc ccgccaccag cgcctcgacc gattccggtt
    33841 ctacgcaaac cgcaataccc ggaaaggttt cgcaaagctg ccccagttct gtgtgggcct
    33901 ctgcggtaat caccgcgtta ccgcctaccg ccagaatatt ggtcagtttc gacggcaata
    33961 cggcatctgc cgcgccgcgt ttttgcacca ccagatggca atcgcccatc ttcagcagtg
    34021 caggtaaagc gtcatacgat tgcagcggga aaaattgcat gttgcgcagt ccacgctgct
    34081 gcgccatttt ttccagccgc gctttgccgc cgccctgccc gacaatggca aaaatcagcg
    34141 gttcatcgcg caggagatcg gcagcttcaa taacgttttc cagcccctgc ttttcaccaa
    34201 tattgccgga gtaaagaatg atttttttgt tatccggcag gccaagctgg ttacgaaggg
    34261 catcaacatc ggcgtctgca acatgctgaa aacgggcaat ttccgaccag ttggggaaga
    34321 agatgacgtt atccgccgcc acgccttttt cgatggcttt attcatcatc gaacgcgaaa
    34381 tcgtggaaac gttatcgacg ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg
    34441 ccactttgcc gcctttgcct tttccggcaa ggcccagccc cagcatggcg tccacttcgt
    34501 aatcctgaat atgcagcacg gtacgcgcac cagagagctt cgccagcagg cgcattcccg
    34561 gcgtgcaaaa cagcgttggc actacgccaa taatgcgatc cggcttccag cgacgttgcg
    34621 ccatcagtgg aaagaaactg ctgacggcaa aactgccgag atgcaacaag cgtttcaggg
    34681 tgctcggctg ttttggcacg tacagcgggc agcgccacac cgtggcggcc ccttcttctc
    34741 gtttgtagcg ccaggcggaa tagttctcac ccacctgcca ttgcgggtag taaggcggtg
    34801 cggtaatgac ccgcacctca tgaccttgtg ccgccaacca ttccaccatc tcgccggtgt
    34861 atttgccgat gccggttaac tccggcgagt agttaatgcc gtagaccagt attttcataa
    34921 tccgggtact ccggcacgct tctcagcgag aaaataggct cggctgttag cgtgaacatc
    34981 gttactggcg agcagcgcgt ccggcgtcag ccagcggtaa tcgtcatgct gctcatccgg
    35041 cagtaacagc tcttcttccg ctactctgaa gcgaaaaccg agcaccacat agtgagtggt
    35101 gaaatccgtg ccggagaagt tatcgtcata aaaatgctgc cagacaccgt aaaactggcc
    35161 tgctgttatc ggcagacgca gccccagttc cgccatcgtc agccgctcaa atgcggcttc
    35221 cagcgtttcg tctttctgca cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg
    35281 gttggttctt ttgccaagca gaaactcgcc gcgactgttc tcgacaataa agtcgagaga
    35341 gacaagcggt gtggagcgca ctacagtggc aaagtcttcc tgacgtaaaa acatcattcc
    35401 ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag tgctggcaag ccccgcttcc
    35461 agtgagattt cgtgatacca gccaagctga tgcaggcgcg tcacatccag cagtttgcgc
    35521 ggcgtaccat ccggtttgct ggcatcaaaa accacccgac ctttgtaacc caccactttg
    35581 gcgatggttt gcgccagctc gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc
    35641 gacagcatcg gttgggtatt ctccagccag acttcatgcg ccagctccat gacatgaatg
    35701 ctcgccgccg ccatatcatc gacgtgcagg aattcacgca tcggtgtacc gctgccccat
    35761 accaccacgt ccggcgcatt ctgtgccgtc gcctcgtgga agcgacgcag caatgctggt
    35821 atcacatggg agttactcgg gtggaagttg tcatgcggcc cgtacaggtt ggtcggcatg
    35881 acagtgcggt aatcgcgtcc atactggcgg ttgtaagatt cgcacagttt gatcccggcg
    35941 attttggcaa tggcgtaagg ctcgttggtc ggctccagcg tgccctgcaa taactcgctt
    36001 tctgccatcg gctgttttgc cagtttcggg tagatgcagg acgatccgag aaacatcagt
    36061 ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc tctcaatcat catgttctgg
    36121 tagatgaaat ccgccggata ggtgttgttg gcaacaatgc cgcccacttt cgccgccgcc
    36181 agatagacct ggtcaatgcg ttcgctggca aagaaatcat gcacggcgcg gctgtccagc
    36241 aggttcagtt catcgcgggt acgtaatacc agctccacat caccgcgctg ttcgagctgc
    36301 cgcttgatgg cagaaccgac catcccacaa tggccagcga taaaaatgcg ttgtttactc
    36361 atgcttatga ctccagcgcg atcgccacgt cgtagccgtg agatttcagc agagagtgtt
    36421 ttttcgccgc ttcgaggtca ttagccacca tttcagacac catctctctg agggtgattt
    36481 ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt
    36541 ctgccggacg gaagtaacgc ggatcaacgg cgataatcac atcacccggt ttaacgcccg
    36601 gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc ttcaacaccc gtgccttcaa
    36661 agcgcagttt gatgcccagc tgtgctgccg ccatttccac aaactgacgt acggagtact
    36721 gaacgccggt cgcgataacg aaatcttccg gctgttcctg ttgcagcatc atccactgca
    36781 tttttacgta gtctttggcg tggccccagt cacgcaggga atccatattg ccgaggtaca
    36841 ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga
    36901 aggtttcgcc gcggcgcggg gattcatggt tgaagagaat tccgttacaa gcgtacatgc
    36961 cgtaggattc gcggtagtta acggtgatcc agtaggcgta cagtttggcg accgcatacg
    37021 gagatcgcgg gtagaacggc gtggtttctt tctgcggaat ttcctgcacc agaccataca
    37081 gttcagaggt ggaagcctga tagaaacgag ttttcttttc cagaccgagg aagcggatcg
    37141 cctccagcag gcgcagcgta cccatcgcgt cgacgtcagc ggtatattct ggtgactcaa
    37201 aagagaccgc aacgtggctc attgcgccta ggttgtacac ttcatccggc tgtacttcac
    37261 gcaaaatgcg cgtcaggtta gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg
    37321 ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg aatgacgatg
    37381 cgcgacgctt aataccatgc acctcgtaac ctttttccag cagaaactct gccaggtaag
    37441 aaccgtcttg tccggttaca ccggtgatga gagcgacttt tgacatgtct tattcctctg
    37501 tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg
    37561 caaaccacat ttgccggaag cgatttaaaa acactgcttc gtgcacccac gacggtgccg
    37621 tcgccgattg tgacgcctgg ggcaacaaag acatcggttg ccagccagca tttctcgcca
    37681 atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg catggtcgtg gctaccggtg
    37741 cataaataac ttttttgcga tatcaccgaa tgtgcgccaa tggttatttc accgagggta
    37801 tataaattga cgtcatcgcc aacccacgcg taatcaccta aggttaattt ccacggatag
    37861 gtaattttta ctgacggacg aataactacg ttttttccta tttttgctcc gaataaacgt
    37921 aataaaaaag cccgccagcg atacaatact tgtggcgacc aggcaaatat tgttgcctgt
    37981 actgcccacc ataattgcac tttaatagcg ttgccgcccc ggaacccttt cggcaccgag
    38041 aaaccgctta aatcttgcat tggttttcct tatattcaga ctttgttata taaggcttta
    38101 gtcttacctg tcgtacgttg gcgtaaatgc caggataatt ctgcccagaa gccaggtaca
    38161 tgtaatatct gccgttggac ttttttg
//
LOCUS       DAJTUP010000029        36930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTUP010000029 DAJTUP010000000
VERSION     DAJTUP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.029"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..557)
                     /gene="yhdJ"
                     /locus_tag="OO468_001524"
     CDS             complement(<1..557)
                     /gene="yhdJ"
                     /locus_tag="OO468_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR6258685.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVR"
     gene            complement(643..939)
                     /gene="fis"
                     /locus_tag="OO468_001525"
     CDS             complement(643..939)
                     /gene="fis"
                     /locus_tag="OO468_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR6258686.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(965..1930)
                     /gene="dusB"
                     /locus_tag="OO468_001526"
     CDS             complement(965..1930)
                     /gene="dusB"
                     /locus_tag="OO468_001526"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR6258687.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2259..3140)
                     /gene="prmA"
                     /locus_tag="OO468_001527"
     CDS             complement(2259..3140)
                     /gene="prmA"
                     /locus_tag="OO468_001527"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR6258688.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3152..4603)
                     /gene="panF"
                     /locus_tag="OO468_001528"
     CDS             complement(3152..4603)
                     /gene="panF"
                     /locus_tag="OO468_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR6258689.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4593..4835)
                     /locus_tag="OO468_001529"
     CDS             complement(4593..4835)
                     /locus_tag="OO468_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR6258690.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(4944..6293)
                     /gene="accC"
                     /locus_tag="OO468_001530"
     CDS             complement(4944..6293)
                     /gene="accC"
                     /locus_tag="OO468_001530"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR6258691.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6304..6774)
                     /gene="accB"
                     /locus_tag="OO468_001531"
                     /gene_synonym="fabE"
     CDS             complement(6304..6774)
                     /gene="accB"
                     /locus_tag="OO468_001531"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR6258692.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(7752..8726)
                     /locus_tag="OO468_001532"
     CDS             complement(7752..8726)
                     /locus_tag="OO468_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6258693.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            8878..10818
                     /gene="csrD"
                     /locus_tag="OO468_001533"
     CDS             8878..10818
                     /gene="csrD"
                     /locus_tag="OO468_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR6258694.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11123..12166
                     /gene="mreB"
                     /locus_tag="OO468_001534"
     CDS             11123..12166
                     /gene="mreB"
                     /locus_tag="OO468_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR6258695.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12232..13335
                     /gene="mreC"
                     /locus_tag="OO468_001535"
     CDS             12232..13335
                     /gene="mreC"
                     /locus_tag="OO468_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR6258696.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13335..13823
                     /gene="mreD"
                     /locus_tag="OO468_001536"
     CDS             13335..13823
                     /gene="mreD"
                     /locus_tag="OO468_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR6258697.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            13832..14425
                     /gene="yhdE"
                     /locus_tag="OO468_001537"
     CDS             13832..14425
                     /gene="yhdE"
                     /locus_tag="OO468_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR6258698.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14415..15884
                     /gene="rng"
                     /locus_tag="OO468_001538"
     CDS             14415..15884
                     /gene="rng"
                     /locus_tag="OO468_001538"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR6258699.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            15952..19752
                     /gene="yhdP"
                     /locus_tag="OO468_001539"
     CDS             15952..19752
                     /gene="yhdP"
                     /locus_tag="OO468_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR6258700.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            19908..21353
                     /gene="tldD"
                     /locus_tag="OO468_001540"
     CDS             19908..21353
                     /gene="tldD"
                     /locus_tag="OO468_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR6258701.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21481..22410)
                     /gene="aaeR"
                     /locus_tag="OO468_001541"
     CDS             complement(21481..22410)
                     /gene="aaeR"
                     /locus_tag="OO468_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR6258702.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22593..22796
                     /locus_tag="OO468_001542"
     CDS             22593..22796
                     /locus_tag="OO468_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR6258703.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            22804..23736
                     /gene="aaeA"
                     /locus_tag="OO468_001543"
     CDS             22804..23736
                     /gene="aaeA"
                     /locus_tag="OO468_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR6258704.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            23742..25709
                     /gene="aaeB"
                     /locus_tag="OO468_001544"
     CDS             23742..25709
                     /gene="aaeB"
                     /locus_tag="OO468_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR6258705.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            25801..26073
                     /locus_tag="OO468_001545"
     CDS             25801..26073
                     /locus_tag="OO468_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR6258706.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26129..26392)
                     /gene="yhcN"
                     /locus_tag="OO468_001546"
     CDS             complement(26129..26392)
                     /gene="yhcN"
                     /locus_tag="OO468_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR6258707.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(26757..27227)
                     /gene="argR"
                     /locus_tag="OO468_001547"
     CDS             complement(26757..27227)
                     /gene="argR"
                     /locus_tag="OO468_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR6258708.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27662..28600
                     /gene="mdh"
                     /locus_tag="OO468_001548"
     CDS             27662..28600
                     /gene="mdh"
                     /locus_tag="OO468_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6258709.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(28662..29729)
                     /gene="degS"
                     /locus_tag="OO468_001549"
     CDS             complement(28662..29729)
                     /gene="degS"
                     /locus_tag="OO468_001549"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR6258710.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(29819..31166)
                     /gene="degQ"
                     /locus_tag="OO468_001550"
                     /pseudo
     CDS             complement(29819..31166)
                     /gene="degQ"
                     /locus_tag="OO468_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31320..31718)
                     /locus_tag="OO468_001551"
     CDS             complement(31320..31718)
                     /locus_tag="OO468_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR6258711.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            31912..33039
                     /gene="zapE"
                     /locus_tag="OO468_001552"
     CDS             31912..33039
                     /gene="zapE"
                     /locus_tag="OO468_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR6258712.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33258..33686
                     /gene="rplM"
                     /locus_tag="OO468_001553"
     CDS             33258..33686
                     /gene="rplM"
                     /locus_tag="OO468_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR6258713.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            33702..34094
                     /gene="rpsI"
                     /locus_tag="OO468_001554"
     CDS             33702..34094
                     /gene="rpsI"
                     /locus_tag="OO468_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR6258714.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34489..35127
                     /gene="sspA"
                     /locus_tag="OO468_001555"
     CDS             34489..35127
                     /gene="sspA"
                     /locus_tag="OO468_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR6258715.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35133..35630
                     /gene="sspB"
                     /locus_tag="OO468_001556"
     CDS             35133..35630
                     /gene="sspB"
                     /locus_tag="OO468_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR6258716.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(35673..>36930)
                     /gene="dcuC"
                     /locus_tag="OO468_001557"
     CDS             complement(35673..>36930)
                     /gene="dcuC"
                     /locus_tag="OO468_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6258717.1"
                     /translation="CGVWLGFGGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIM
                     AVGGYARYMERIGASCAMVSLLSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGM
                     LLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQ
                     LPVASCVIISVAISHFFVQRAFDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILM
                     LGSLFLAHVGLMQSELHLVVVMLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQF
                     VNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSG
                     NAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPF
                     AVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8926 a         9256 c         9748 g         9000 t
ORIGIN      
        1 cgcacgcgcg gaaaatccca gacgttaccc ggtacttttt gatgattgta tggctgtgga
       61 ggatttttgc gataatcgat caacgcgcgc tgcgatccgg ttttggcttc taccagaata
      121 gcatcaccgt tgaatgtgta gttctttgcg tctttcacca tcatcaggat gggttcgtac
      181 atggagccgt agtgtttttt cgcctgtact ccagaactgt catatgacca gacgatgcga
      241 cttttgatgg taaaaagctt gcggcactgg agatcgataa agggcatgtt ttccgtactg
      301 ttcataatgt acatgctgcc ctgctttttc agaacgcggt ggcactctgc aatcacttca
      361 aacagccagt cgataaacag atcttctttc caggcttcga tcagaccatc aaaattttta
      421 ccgatgttat acggtgggtc ggcaaagagc agatcgacgc tttcagtagg tagcttttta
      481 agttcggcaa aggcatcacc gtgaataatg gtcttagctt cattaccaaa ccgggtcggt
      541 tcacatcctg ttctcatggt cactcccttt gtgacaccta taaaaaaggc gcttccccat
      601 gccgagtagc gcctttttaa tcaagcattt agctaacctg aattagttca tgccgtattt
      661 tttcaatttt ttacgcagcg taccacggtt gatgcccatc atcagcgcag cacgggtctg
      721 gttaccacgg gtgtattgca tcaccatgtc caacaggggc tgttctactt cagccagtac
      781 cagctcatag aggtcattca catcctgacc attcagttga gcaaaatagt tcttcagtgc
      841 ctgtttaacc gagtcacgca ggggtttttg ggttacctga tcctgagagt taacggtaga
      901 aacggtcagt acgtcagaat ttacgcgttg ttcgaacata gttctgtcag ctctttattt
      961 ctgtttacgc aaaattttcg aagtatgcct ccaacgcctc cagctgttcg ctggcatcct
     1021 caatggcgtt gaatgtgcgc cgaaactggt catttggagc gtgttcctgg agataccagg
     1081 aaacgtgttt acgtgcaatt cggtaccctt ttgccggacc ataaaagtca tgcagttccc
     1141 gaacgtgcgc gcaaagcaag cgcttaacct ctgccaaagg cagtgggggc agcaactccc
     1201 cagtgtccag ataatgctgg atttcccgaa agatccaggg tcttccctga gctgcgcggc
     1261 ctatcatcag ggcatccgcc cctgtatagt cgagcacagc tctggcttta agcgggtcag
     1321 taatgtcgcc attcgcgata accggaatgg aaactttctg cttaactgcc cgaatactgt
     1381 cgtactcagc ttctccattg aacaaacagg cgcgtgtacg gccatgaatg gtcagagcct
     1441 gaatgccaca gtcttcagcc agttgggcaa tctcttcgca gttacggtgt tccggtgccc
     1501 agccggtgcg aatcttcagg gtaacaggaa cgtccactgc attgacgacc tcggtaagga
     1561 tcgatttaac gacatccggg tactgcaaga gggctgaacc tgcgagcttg cgattcactt
     1621 ttttagccgg gcaacccata ttgatatcaa taatctgggc accgctttcc acgttaatac
     1681 gtgctgcatc tgccatttct ttcggagcgc taccagcaat ttgcacggtg cgaataccgg
     1741 gttcatcaat gtgcaccatc cgtaaacgag atttgtcgct ttcccaaacc tgtgggttag
     1801 aagacatcat ctcggatact gtcaatccgg ctcccatctc gtagcacaac gtccgaaaag
     1861 gtctgtctgt aatgccagcc atgggcgctg cgatcaggcg atttctgagc tgatattgtc
     1921 cgatgcgcat gagttaagaa atgaccatac tgtgactgca aggcggcgta tattacgcat
     1981 tttttgcacg agatgaaagg ccaaactttg accaatcctc tgagatggat caaagaattg
     2041 catttaaaat gagcgtggtg cgataattac tcataaaaat catcatatta gaaaatagtg
     2101 actaaaattt acactcaaag aaatttgagt aagttctcaa tttttcttta tgaatgaaaa
     2161 ttttggcacg caaatttgcg taaataatcg gcaaatttac gtgccttttg tgagcttgct
     2221 cgcacttcgc cccgcgtcac cctacggcga tgcgaaggtc aattcttacg accggtaata
     2281 cggcaccact cttctttttc cacgaccggg tccagtgcga agctatcggc ataagcttca
     2341 caaacgctct ctgcctggct tgccagaata ccggaaaggc ccagcaaacc gcctgaaact
     2401 ggcaggacgc tgattaacgg tgccagttca cgtaatgggc ctgcaaggat gttagcgacc
     2461 accacgtcgg ctttcatttc ttctggctga tctttcggta agtagagttc cagacggtca
     2521 gaaacgccat tacgttcggc gttatcgcgg ctggcctgaa tcgcctgcgg atcgatatca
     2581 ataccaatgg cttttgctgc acccagtttc agcgccgcga tcgccagaat gccggaacca
     2641 cagccaaagt cgatgactgt tttaccggtt aaatcgaggc tgtcgagcca ttgcaggcac
     2701 agagaggtgg ttggatgggt acccgtacca aacgccagcc ctggatctaa catcacgttg
     2761 acggcgtttt cgtccggcac atcacgccag ctagggcaga tccacagtcg ttcaccaaag
     2821 cgcatcgggt ggaaattatc catccattcg cgctcccagt ctttatcttc tagttgttcg
     2881 attttatgcg cgaagcctgc gccgagcagc ggatggtttt ccagaatcgc caccacgtcg
     2941 ttcatatcgg tttcagcgtc gaacagacca atcacatcgg tgtcgcccca caggcgcgtt
     3001 tcgcccggca gcggctcaaa tactggcgta tcgtgggtat cctgaaaagt gatagaaacg
     3061 gcacccgctt ccatcagcgc atcgctaaga tcttccgcgt tcgcgccggt ggtgttcagt
     3121 ttcagttgga tccaaggcat ggcaaaactc tttatttatc agtagtcaaa acggtagctt
     3181 gcgggacgga tgtaccgaaa cggtttccga ccaggaaagc cagcaaactt agtagtaacg
     3241 agggcacgat agggtggaag cccaggtact gaatattcag cgtcgcgagt acggcataca
     3301 gcacgccgcc aacgatcatc gcacttagcg cgcctttggc gttggcgcgt tcccagtaaa
     3361 gacccagcac cagcggccac aggaaaacgg cttccagccc accgaaggcc agcaaattca
     3421 gccagatgat catttctggc ggcttccagg cggcaagcag cagcaacgcg ccgagaacta
     3481 acgtaattac cgccgacatc cgcttcagac gcgtctcgtt ttgcatttga tccggacgga
     3541 tattcagata gagatcttta atgatcgtag cggaactttg cagcagttgg gcgttaattg
     3601 tcgacatgat cgcagccata ggtgcagcca gaaagatccc ggcagcaaaa ggtggcagca
     3661 cttttaccat taacgtcggg atcaccagat ctggtacggt gagatcgggg atcactgcac
     3721 gacctaacgc tccggccagg tgcataccga acatcaggat tgcgactata atcgtaccga
     3781 tgatgatccc ccggtgtact gctttgctgt ctttataaga gatacagcgc accgcagtat
     3841 gcggcaggcc aatcacgcca aagcacacca gtacccagaa cgacgtcata aaggcaggcg
     3901 acagaatatc gtcagcgcct tgtggcgtaa ccagttgcgg atcgatggtt tgcaaggtct
     3961 gtactgcgtt gcttaagccg ccagcggcat gtaccacgcc aataagcagc acaacggtgc
     4021 caatcagcat cacaagccct tgcatggtgt cgttcagcac gctggcgcga aagccaccaa
     4081 aggcggtata taacgcaatg ctgataccaa aaatcagcag cccggtttca taaggaatac
     4141 ccgccgcggt ttccagcagg cgcgcaccgc cgataaactg cacggtcatc gcaccaacga
     4201 acgcaaccag caaactcaaa ctcgccagcc acaccagaag acgactctgg tagcgggcaa
     4261 acagcatatc gttcagcgtc actgcattgt agcggcgcgc aagaatcgca aacttcttgc
     4321 cgagaatacc gagtgaaagc cagacagcag gaagctgaat catcgccagc aatacccagc
     4381 ccagcccgta tttataagca gctcctggcc cgccgataaa cgaactggca ctgatatagg
     4441 tcgcggtgag agtcatcgcc agcacaatac cgcccataga gcggctgccg aggaaatact
     4501 cattaaggaa ggtgccggtg ctccgtttac gcatcgcata aaccgagata ccgaacacca
     4561 ccaccagata ggcgaccagc ggtagaatta cttcaagctg catcgtcatc ctccagtggg
     4621 atatcgcgat agataaattt caccatcgcc cagcacagtc caataaacag tagcggcgtc
     4681 aggatgcagg ccatctcaaa ccagcgcgga aagccggtaa aaccgggggc aacgccaggt
     4741 aagtaagcgt ctactaacca aactgccaga tacaaaaggg tcagccccag cgcccagcgg
     4801 gcctctttat gggcctgaac aaaacgagtg tccatttttt gtccctgatg ggtgaagaaa
     4861 gcgaggattg taccttatgg gggttgtcga tccccagtaa taaaaaaggc cggaaaatcc
     4921 ggccttttga cgcttttgca gtcttatttt tcctgaagac cgagtttttt ctccagatag
     4981 tggatgttag tgccaccatg ctggaagttc tcgtcattca tgatgcggat ctgcagatca
     5041 acgttggttt tgataccgtc gatgatcagc tcctgcagcg cattcttcat gcgggcaatc
     5101 gccacgtcac ggttttcacc gtagcaaatc agcttaccga tcattgagtc atagtacggc
     5161 ggtacggtgt agcccgcgta gatatgagac tcccaacgta cgccaaaacc gccaggtgcg
     5221 tggaaacggg tgattttgcc tgggcttggc aggaaggtgt tcggatcttc ggcgttgata
     5281 cgacattcca ccgcatggcc gcgaacgtga acttcttctt gcttgatcga cagcggttga
     5341 ccggcagcga tacgcagctg ttctttgatc aggtcaacgc cggtgatcat ttctgtaacc
     5401 gggtgttcta cctgaatacg ggtgttcatt tcgatgaaat agaactcgcc gttttcgaac
     5461 aggaactcga aagtacctgc accgcgatag ccgatatcaa cacacgcttt agcgcaacgt
     5521 tcgccgatgt agcgacgcag ttccggggta atgcccggtg ctggtgcttc ttcgaccact
     5581 ttctggtggc ggcgctgcat ggagcagtca cgttccgcca gatagatagc gttgccctga
     5641 ccgtcagcca gtacctgaat ctcgacgtgg cgaggattct ccaggtattt ctccatgtaa
     5701 accatatcgt tgctgaaagc agctttcgct tccgcacggg tcatggagat ggattgtgcc
     5761 agttcagcgt cgccgcgcac tacgcgcata ccgcgaccgc cgccgccgcc ggaggctttg
     5821 ataatcaccg gataaccaat gcgtttagca atggcgcggt ttttatccat atcgtcgccc
     5881 agcgggccgt cagaaccagg tacgcaagga acgcccgctt ttttcatcgc cgcgattgcg
     5941 gatactttgt cgcccatcag gcgaatggtt tctgctttcg ggccgatgaa gataaagccg
     6001 gagcgttcaa cctgctcggc aaagttggcg ttctcggaga ggaagccgta acccggatgg
     6061 attgctactg cgccggtgat ttcagcggcg ctgatgattg ccgggatgtt cagataactt
     6121 tttactgacg gagcagggcc aatacagacc gtttcatctg ccagtaatac gtgttttaga
     6181 tcgcgatccg tgctggagtg cacagcgaca gtcttgatgc ccagttcttt acaggcacga
     6241 agaatacgca atgcaatctc gccgcggttg gcaataacaa ttttatccag catgttcgcc
     6301 tcgttactcg atgacgacca gcggctcgtc aaattctacc ggttgtccac tttcgaccag
     6361 aattgctttc acggtaccgg atttgtccgc ttcgatctgg ttcatcattt tcatggcttc
     6421 aacgatgcac agggtatcgc ccacgttgac tttctgaccc acttcgatga acgcttttgc
     6481 gtccgggctt ggggtgcggt agaaagtacc aaccatcggg gaacgtacga tgtgaccact
     6541 gatttccgct gctgctggcg cttccatgga aggaacggtc gccggagcgg ctgcgttaga
     6601 ttgagctggc tgctgcatca ttggtgcagc gtaagcttgt tgcatcacag ggaaacttgc
     6661 ggcaggagct gcacggctaa tgcgtactga ctcttcgcct tcagaaattt ccagttcgga
     6721 gatgcctgat tcttcaacca gctcgatcag ttttttaatc ttacgaatat ccatgagtgg
     6781 gttccgtact ctttgtttag tgtgattgtg acaggcgttt caccgccgtc tgtaaagcgt
     6841 atgccccgac agctgtatgc atagcgataa attccagcag gccgggacgc cggtctattt
     6901 tgccccagag tgtcaatgtt agacttgacg gacattgtgc agcgatctgc tgctatcctc
     6961 cggcaaaaaa caaaatatac cttcgttgcg ccaatgtcac cttttccgca gcgcaaaaac
     7021 tgcgtcaggg aggacgaggt cgcacattat aacgatttcg tagcaattgg cagctaaata
     7081 ctggtcttat cagggaatat aatcaacagc taacatgtaa ataaccttca acaccgtgta
     7141 atttgcaaca agccgcacaa ttcacgaaat tagcgccatc atcgacggaa cttcttataa
     7201 cgtaaggtga aaagctgaaa agccagccct gcgaagatga ggggctgcaa tgagataatc
     7261 ttcacagacc acaaataatg tagggatgcc aggatcgcga caagatagac gacattacgc
     7321 agcggttgct ggggattacc caattttcac tgcatcgtct ggatagatgt gaatgctaaa
     7381 gcacgcaaaa tcaaccaact gacaattctc agcattaaat aggttcgagt ggttaatttg
     7441 tagtcaaata aaatcagatt attcacccca tggtaccaga aatcagtaac aggttaagcg
     7501 tagtgtcgcc caggcaaagc accataaacc taacaggcga cggaggcgta tcaataatgg
     7561 ctgtttagag gagtatcaat gcgcatcaat ttgtggtcag tcgaaatcat gactttccag
     7621 tttggatgat gttgtacaac ttcctgacca aggtgttcga cgaaagctgt ccggctaacc
     7681 gcttttcccg ttaaccgatt gaataatgat gtcaaaatga gttccctctc ttattattcc
     7741 ttgctaaatg gttagttaac cttcaccagc gtgcgaccct ggatctggtt attaatgatg
     7801 gcctcggcaa agttcggtgc ctctgacaga gatatctctt ttgccgcctg ggtatagaat
     7861 gattccggta aatcggcgac cagtcgctgc caggcttgtg cgcggcgttc tggtggcgtc
     7921 attactgaat ccaccccttg caaacggaca ttacgcagaa taaatggcat gaccgtggtt
     7981 ggcagagtaa aaccacccgc cagaccacag gccgccacgc agccgccgta attcatttgc
     8041 gccagcactt ttgccagcac tttgtcgcca acagtgtcaa ttgccccagc ccagacttgt
     8101 ttttccagag gacgggattc ggcaaactca tcacgaggga gaataccgct ggcacctaaa
     8161 cttttcagat attcatgggt actttcgcga ccggaaacgg caacgacctg ataacccaac
     8221 ttatgcagca gcgccacggc ggtactgccg acgccaccac tggcacccgt cacgacaatc
     8281 tcaccgtcct gcgggcgaac accggcatct tccagcgcca tcacacacag catggcggta
     8341 aaaccggcag taccgataat cattgcttta cgcgcgtcca gcccttgcgg catggcaacc
     8401 agccagtcac ctttcactcg cgcctgctcc gccagcccac cccagtggtt ttcaccaacg
     8461 ccccagccag tgagtaacac ctcctgaccg gcatgaaaac gcggatcttc gctggtgcgt
     8521 acagttccgg caaaatcgat cccaggaatc atcggaaaat tacggatgat ttttccctta
     8581 ccggtaatcg ccagcgcatc tttatagttc aggctcgacc agtgaacatc gaccgtgaca
     8641 tcgccctccg gcaggcgacg ttcgtccaga gtctgtactg atgcgagagt tttgccgtcc
     8701 tgctgttcta aaagtaacgc ctgcataagt ggtcctcatg tgcatgatgg attggaaaat
     8761 aatttctgaa gactatactc gctaatggaa aagcaaagcc gatgaagcgc aataaattgc
     8821 gagataaatc tgatttgcta gtatgcccgc ttcctcacta tcggagttaa cacaaggatg
     8881 agattaacga cgaaattttc ggcctttgtt acgctgctca ccgggttaac aatttttgtg
     8941 actttgctgg gctgttcgct aagtttctac aacgccattc agtataagtt tagtcatcgc
     9001 gttcaggcgg tggcgacggc gattgatacc caccttgtgt cgaatgactt cagcgcatta
     9061 aggccacaaa ttaccgaatt aatggtgtca gcagatatcg ttcgtgtaga cctgctgcat
     9121 ggtgataaac aggtttatac cctggccaga aatggtagtt atcgtccggt tggcaccagc
     9181 gatctgtttc gtgaactgag cgttccgttg ataaagcatc cggggatgtc gttgcgtctg
     9241 gtttatcagg atccgatggg caactatttc cattcgttga tgaccaccgc gccgctcacg
     9301 ggggcgattg gctttatcat tcttatgctc tttctggcgg tacgctggtt acaacggcaa
     9361 cttgccgggc aagaattgct ggaaacccgg gctactcgta tcttaaacgg tgagcgtggc
     9421 tctaatgtgt tgggaaccat ctatgaatgg ccgcccagaa ccagcagtgc gctggatacg
     9481 ctgcttcgtg aaattcagaa cgcacgcgaa caacacagcc gtcttgatac gctgatccgc
     9541 tcttatgccg cacaggacgt gaaaaccggc ctcaataacc gactcttttt cgataatcag
     9601 ttagcaacgt tactggaaga tcaggagaaa gtaggtaccc acgggatcgt gatgatgatt
     9661 cgtctgccgg atttcaatat gttgagcgat acctgggggc acagccaggt tgaagaacag
     9721 ttcttcactc tgacgaatct gctgtcgaca tttatgatgc gctaccctgg cgcactgctg
     9781 gcgcgttacc accgcagtga ttttgctgcg ctgttaccgc accggacgtt aaaagaggca
     9841 gagagcatag ccagtcagtt aatcaaagcc gtcgatacct tgccgaacaa taaaatgctc
     9901 gatcgcgacg atatgatcca cattggtatc tgtgcctggc gtagtggtca ggataccgag
     9961 caggtaatgg aacatgcaga gtctgccacg cgtaatgcgg gattgcaggg tggcaatagc
    10021 tgggctattt atgatgactc gttgcctgaa aaaggacgcg gtaatgttcg ctggcgtacg
    10081 cttatcgagc aaatgctcag tcgcggcggc ccgcgccttt atcaaaaacc ggcggttact
    10141 cgcgaaggtc aggttcatca tcgcgaactc atgtgccgca tcttcgatgg taatgaagag
    10201 gttagctcgg cggagtatat gccgatggtc ttgcagtttg gcttatcgga agagtatgac
    10261 cgtctgcaaa tcagccgtct tattccacta ttgcgttact ggccagagga aaatctggcg
    10321 attcaggtta ccgttgagtc gctgattcgt ccgcgttttc agcgttggct gcgcgatacg
    10381 ttaatgcaat gtgaaaaatc acaacgaaaa cgcataatta ttgaacttgc agaggccgat
    10441 gtaggtcaac atatcagtcg tttacaacct gttattcgtt tagtgaatgc tttaggggta
    10501 cgggtagccg tcaaccaggc tggtttgacg ctggtaagca ccagttggat caaagaactt
    10561 aatgttgagt tactcaagct ccatccgggg ctggtcagga acattgagaa gcgaacggag
    10621 aaccagctgc tggttcaaag cctggtggaa gcctgctccg ggaccagcac ccacgtttac
    10681 gccaccggcg tgcgttcgcg aagcgagtgg cagaccctga ttcagcgcgg tgttacaggc
    10741 gggcaagggg attttttcgc gtcctcacag ccacttgata ctaacgtgaa aaaatattca
    10801 caaagatact cggtttaacc tgccgtttaa tccgttttca cgtagaataa cgcgcgctgc
    10861 gtctcatggg agtgtgcttg tctgctcgcc agattgttgc agcacatatg cagatgaatg
    10921 accttacgcg gttgcaaaca ggcgaggaat gctgctgatg cattaagcct ttctggactc
    10981 aggcagagat ttgtaacaaa ggaaacgaac tgcactaatt ttcaccgtag cagatgattt
    11041 ttgcgccttg tcgctgctgc gtgtggttgg taaagtaagc ggattttctt ttccgcccca
    11101 gctttcagga ttatccctta gtatgttgaa aaaatttcgt ggcatgtttt ccaatgactt
    11161 gtccattgac ctgggtactg cgaataccct catttatgta aaaggacaag gcatcgtatt
    11221 gaatgagcct tccgtggtgg ccattcgtca ggatcgtgcc ggttcaccga aaagcgtagc
    11281 tgcagtaggt catgacgcga agcagatgct gggccgtacg ccgggcaata ttgctgccat
    11341 tcgcccaatg aaagacggcg ttatcgccga cttcttcgtg actgaaaaaa tgctccagca
    11401 cttcatcaaa caagtgcaca gcaacagctt tatgcgtcca agcccgcgcg ttctggtttg
    11461 tgtaccggtt ggcgcgaccc aggttgaacg ccgcgcaatt cgtgaatccg cgcagggcgc
    11521 tggtgcccgt gaagtcttcc tgattgaaga accgatggct gccgcaattg gtgctggcct
    11581 gccggtttct gaagcgaccg gttctatggt ggttgatatc ggtggtggta ccactgaagt
    11641 tgctgttatc tccttgaacg gcgtggttta ctcctcttcc gtgcgcattg gtggtgatcg
    11701 tttcgacgaa gctatcatca actatgtgcg tcgtaattac ggttctctga tcggtgaagc
    11761 caccgcagaa cgtatcaagc acgaaatcgg ttcggcttac ccgggcgatg aagtccgtga
    11821 aatcgaagtt cgtggccgta acctggcaga aggtgttcca cgcggtttta ccctgaactc
    11881 caacgaaatc ctcgaagcac tgcaggaacc gctgaccggt attgtgagcg cggtaatggt
    11941 tgcactggaa cagtgcccgc cggaactggc ttccgacatc tccgagcgcg gcatggtgct
    12001 caccggtggt ggcgcactgc tgcgtaacct tgaccgtttg ttaatggaag aaaccggcat
    12061 tccagtcgtt gttgctgaag acccgctgac ctgtgtggcg cgcggtggcg gcaaagcgct
    12121 ggaaatgatc gacatgcacg gcggcgacct gttcagcgaa gagtaatcgg atgcaggcag
    12181 gggaagcgtc tgtttaccct gcctggtctg atacgagaat acgcataact tatgaagcca
    12241 atttttagcc gtggcccgtc gctacagatt cgccttattc tggcggtgct ggtggcgctc
    12301 ggcattatta ttgccgacag ccgcctgggg acgttcagtc aaatccgtac ttatatggat
    12361 accgccgtca gtcctttcta ctttgtttcc aatgctcctc gtgaattgct ggatggcata
    12421 tcgcagacgc tggcctcgcg tgaccaacta gaacttgaaa accgggcgtt acgtcaggaa
    12481 ctgttgctga aaaacagtga actgctgatg cttggacaat acaaacagga gaacgcgcgt
    12541 ctgcgcgagc tgctgggttc cccgctgcgt caggatgagc agaaaatggt gactcaggtt
    12601 atctccacgg ttaacgatcc ttatagcgat caagttgtta tcgataaagg tagcgttaat
    12661 ggcgtttatg aaggccagcc ggtcatcagc gacaaaggtg ttgttggtca ggtggtggcc
    12721 gtcgctaaac tgaccagtcg cgtgctgctg atttgtgatg cgacccacgc gctgccaatc
    12781 caggtgctgc gcaacgatat ccgcgtaatt gcagccggta acggttgtac ggatgatttg
    12841 cagcttgagc atctgccagc gaatacggat attcgtgttg gtgatgtgct ggtgacctcc
    12901 ggtctgggcg gtcgtttccc ggaaggctat ccggtcgcgg ttgtctcttc cgtaaaactc
    12961 gatacccagc gcgcttatac tgtgatccag gcgcgtccga ctgcaggact gcaacgtttg
    13021 cgttatctgc tgctgctgtg gggcgcggat cgtaacggcg ctaacccgat gacgccggaa
    13081 gaggtgcatc gtgttgctaa tgaacgtctg atgcagatga tgccgcaggt attgccttcg
    13141 ccagacgcga tggggccaaa gttacctgaa ccggcaacgg ggatcgctca gccgactccg
    13201 cagcaaccgg cgacaggaaa tgcagctact gcgcctgttg cgccgacaca gcccgctgct
    13261 aatcgctctc cacaaagggc tacgccgccg caaagtggtg ctcaaccgcc tgcgcgtgcg
    13321 ccgggagggc aatagtggcg agctatcgta gccagggacg ctgggtaatc tggctctctt
    13381 tcctcattgc gctgttgctg caaatcatgc cctggccgga taacctgatt gttttccggc
    13441 caaactgggt gttacttatc ttgttgtatt ggatcctggc cttgccccat cgcgtaaatg
    13501 tgggcacagg ttttgtgatg ggtgccatac tggatctgat cagcggctcg atgcttggcg
    13561 tacgcgtatt ggcgatgagc atcattgctt acctggtggc gctgaaatac cagcttttcc
    13621 gcaacctcgc attatggcag caggcgctgg tcgtcatgtt gctttcgctg gtggtggata
    13681 ttattgtttt ctgggcagag tttttagtga ttaacgtctc tttcagaccg gaagtgttct
    13741 ggagtagtgt agtcaatggg gtgctctggc cgtggatttt cttgctgatg cgtaaagtcc
    13801 gtcagcagtt tgcagtgcaa taaaggtttc tatgacttct ctgtatttag cttccggttc
    13861 tccgcgtcgt caggagttac ttgcgcaact tggcgtgacc tttgaacgta ttgttacggg
    13921 cattgaggag cagcgtcagc cgcaggagag cgcgcagcag tatgttgtgc gtctggcacg
    13981 cgagaaagca caggcgggtg tcgcgcaaac ggcacaggat ctcccggtgc tgggtgcgga
    14041 tattatcgtt atcctgaacg gagaagtgct cgagaaaccg cgcgacgcag agcatgcggc
    14101 gcagatgttg cgcaaattat cgggtcagac ccatcaggtg atgacggcag tggcgttggc
    14161 cgacagccag cacattctcg attgcctggt ggtcaccgat gtgactttca gaacgttaac
    14221 agacgaagac atcgcgggct atgtcgccag cggtgaaccg ttagataaag caggtgcata
    14281 cggtattcag gggctgggtg gctgttttgt caggaagata aatggcagct atcacgccgt
    14341 agtcggctta ccgctggttg aaacgtatga attattaagt aattttaacg cactgcgtga
    14401 gaaaagggat aaacatgacg gctgaattgt tagtaaacgt aacgccttcg gaaacgcgag
    14461 tggcgtatat tgatggcggt attctgcagg aaattcatat tgaacgtgag gcgcgacgcg
    14521 gaatagtagg caatatctac aagggtcgtg taagtcgtgt acttccgggt atgcaggcgg
    14581 cttttgtaga tattgggctg gataaagccg cgtttcttca tgcatccgac atcatgccgc
    14641 ataccgaatg tgtggcgggt gaagaacaaa agcaattcac ggtgcgcgac atctcggaac
    14701 tggttcgtca ggggcaagat ctgatggtgc aggtggtgaa agatccgctt ggcactaaag
    14761 gtgcgcgcct gaccaccgat atcacgctgc cttctcgcta tctggtattt atgccagggg
    14821 cttctcacgt tggggtttcc caacgaattg aaagcgaatc agaacgtgaa cgtctgaaaa
    14881 aagtggtcgc agagtattgc gacgagcagg gcgggtttat catccgcacc gcagcggaag
    14941 gggttggcga ggctgaactg gcctccgatg ccgcttatct gaaacgcgtc tggaccaaag
    15001 ttatggagcg taaaaagcgc ccgcagaccc gttatcagct gtacggcgaa ctggcgctgg
    15061 cgcagcgtgt tctgcgtgat tttgccgacg ccgaactgga ccgcattcgc gttgactcac
    15121 gcctgactta cgaagcattg ctggagttta cctcggagta cattcccgag atgaccagca
    15181 agctggagca ttacaccgga cgccagccga ttttcgatct ctttgatgtc gaaaacgaaa
    15241 tccagcgagc gttggaacgc aaagtagaac tgaaatccgg tggttatctg attatcgacc
    15301 agaccgaagc gatgaccacc gtggacatca ataccggagc gtttgtcggt catcgcaatc
    15361 tggacgacac tattttcaat accaatattg aagcgacgca ggctatcgct cgccagttac
    15421 ggttgcgtaa tctgggcggg attatcatta ttgatttcat cgatatgaat aatgaagatc
    15481 accgccgccg cgtgctgcat tcgctggagc aggcgttgag caaagaccgg gtgaaaacca
    15541 gcgttaatgg tttttcggcg ctggggctgg tggagatgac gcgtaaacgc acccgcgaaa
    15601 gcattgagca cgtactgtgt aacgaatgcc caacctgcca cggtcgcgga acggtgaaaa
    15661 ccgtggaaac ggtatgctat gaaatcatgc gcgagattgt tcgtgtccac catgcttacg
    15721 actccgaccg tttcctggtc tatgcttctc cggcagtagc cgaagccttg aaaggcgaag
    15781 agtcacactc gctggcggaa gtggaaattt tcgttggcaa acaggttaaa gtacaaattg
    15841 aaccgctcta taaccaggag cagtttgacg tcgtaatgat gtaaacagat gctgggccgc
    15901 catccggcaa agggtttttg agtcacattt ttagcagaca aggagtgacg ggtgaggcga
    15961 ttgccgggga ttttactgct tactggagcc gcgctcgttg tgatcgctgc cctgctggtt
    16021 agcggcctgc gtattgcttt accgcatctt aacgcctggc gtccggaaat cctcaacaaa
    16081 atagaatccg cgactggcat gccggtagaa gccagtcagc tctcagccag ctggcagaat
    16141 tttggcccga cgcttgaagc acacgacatc cgtgcagaac taaaagatgg cggcgaattt
    16201 tcggttaaac gcgttactct ggcgctggat gtctggcaga gcctgttaca tatgcgctgg
    16261 cagtttcgcg acctcacttt ctggcagctg cgctttcgca ccaacactcc tatcaccagc
    16321 ggtggtagcg atgacagtct ggaagccagt cacatcagcg atctgtttct tcgtcaattt
    16381 gaccatttcg atctccgcga cagtgaagtc agttttctga cgccatccgg tcagcgcgcc
    16441 gagctggcga tcccacaact cacctggctg aacgatccac gtcgacaccg tgcggaaggc
    16501 ctggtaagcc tctccagcct taccggacag cacggcgtga tgcaggtgcg catggatttg
    16561 cgcgatgatg aggggttgtt aagcaatggt cgcgtctggc tacaggcgga tgacatcgac
    16621 ctgaagccgt ggctcggtaa atggatgcag gacaatattg ctctggaaac ggcacagttc
    16681 tcccttgaag gctggatgac gatcgacaaa ggcgatgtaa ccggcggtga cgtctggctg
    16741 aaacagggcg gtgccagctg gttgggcgag aagcaaacgc atacgctgtc ggtggataat
    16801 ctgaccgcgc atattacgcg tgaaaatccg ggctggcagt tctctattcc cgatacacgg
    16861 atcacgatgg acggcaaacc ctggccgagc ggagcattga cgctggcctg gataccggaa
    16921 caggacgttg gcggcaaaga caataaacgc agtgacgaac tccggattcg cgctagtaat
    16981 ctggagctgg caggcctgga gggcatacgc ccgctggccg cgaaactttc acctgcactg
    17041 ggtgatgttt ggcgctccac acaaccgagc ggcaagatta acactctggc gctggatatc
    17101 ccgcttcagg cggcagacaa gacccgtttt caggcatcgt ggagcgatct ggcctggaag
    17161 caatggaaat tattaccggg tgcggaacac ttctccggga cgctttccgg cagcgttgaa
    17221 aatggtttgc ttaccgcgtc gatgaagcag gcaaagatgc cttacgaaac ggtattccgt
    17281 gcgccactgg aaatcgccga cggccaggca actataagct ggctgaacaa tgacaaaggt
    17341 ttccagctgg atgggcgtaa tattgacgtt aaagccaaag ccgtccatgc gcgcggcggt
    17401 tttcgttacc tgcaacctgc taacgatgaa ccctggctgg gtattctggc tggcatcagt
    17461 accgatgatg gttcacaagc ctggcgctat ttcccggaaa acttgatggg taaagacctg
    17521 gttgattatt taagtggtgc gattcagggc ggtgaagcgg ataacgcgac gctggtttat
    17581 ggtggcaatc cgcaactctt cccctataaa cacaacgaag gtcagtttga agtgctggtg
    17641 ccgctgcgca acgcgaagtt tgccttccag ccggactggc ctgcattaac taaccttgat
    17701 attgaactgg actttattaa cgacggttta tggatgaaaa ccgatggcgt taatctgggc
    17761 ggcgtgcgcg cgagtaatct caccgcagtg atccctgact actcaaaaga aaaactgctg
    17821 attgacgctg acattaaagg tccgggtaaa gccgttggcc cttactttga tgagacaccg
    17881 ctgaaagatt ctctgggtgc gaccctgcaa gaactccagc tcgacggcga tgtgaatgct
    17941 cgcttacatc ttgatatccc gctgaacggc gaactggtaa ccgcgaaagg tgaagtgacg
    18001 ctgcgtaata acagtctgtt tatcaaacca ctcgacagca tcctgaaaaa tttgagcggt
    18061 aaattcagct ttatcaatgg cgatctgcaa agtgaaccac tgacagcaag ctggtttaat
    18121 cagccgttga acgtggattt ttccaccaaa gaaggggcaa aagcctacca ggttgcggtg
    18181 aatctcaacg gtaactggca accggcgaaa accggcgttc tgcctgaagc ggtgaacgaa
    18241 gcattgagtg gcagcgtggc gtgggatggt aaagtgggca ttgatctgcc ttatcatgct
    18301 ggcgcgacct ataacgtaga gctgaacggc gatctgaata atgtgagtag tcacttacct
    18361 tcaccgttag ccaaacctgc gggtgaacca ctagcggtaa acgttaaggt tgatggcaat
    18421 ctcaacagct ttgaattaac cggacaggct ggtgcggata accatttcaa tagccgctgg
    18481 ttgcttggtc aaaagctgac gctcgatcgt gctatttggg cggcagacag taaaacgctc
    18541 ccgccgttgc cggaacaaag tggcgttgaa cttaatatgc cgccgatgaa tggtgccgag
    18601 tggctggccc tgttccagaa aggcgctgcg gagagtgtcg gtggtgcagc gagtttccca
    18661 caacacataa cgttacgtac gcctatgttg tcactgggaa atcagcaatg gaataacctg
    18721 agtattgttt cgcaaccgac ggcaaatggc accctggttg aggcgcaagg gcgtgaaatc
    18781 aacgccacgc tagcgatgcg taataacgcg ccgtggctgg cgaatatcaa atatctttat
    18841 tacaacccga gcgtggcgaa aactcgtggt gattcaacgc cgtcatcacc tttcccgaca
    18901 acggagcgca ttaacttccg tggctggccg gacgcacaaa tacgatgcac agagtgctgg
    18961 ttctgggggc aaaaattcgg tcgcattgac agtgatatca ccatttctgg cgatacgtta
    19021 acgctgacca atggactgat tgatactggt ttctcgcggc ttactgccga tggtgaatgg
    19081 gttaataatc cggggaatga acgtacctcg ctgaaaggaa aactgcgtgg gcagaaaatt
    19141 gatgccgccg cagaattttt tggtgtcacg acgcccatac gccagtcgtc atttaatgtg
    19201 gattacgatt tacactggcg caaagcaccc tggcagccag atgaagcgac gttgaatggc
    19261 atcattcata ctcaactggg taaaggcgaa attaccgaaa tcaataccgg acatgccggg
    19321 caattgctgc gcttattgag cgtagatgcc ctgatgcgta agctgcgttt tgatttcaga
    19381 gacacttttg gcgaagggtt ctattttgac tccattcgca gcaccgcgtg gattaaagac
    19441 ggcgttatgc acaccgacga cacgctggtg gatggcctgg aggcggatat cgccatgaaa
    19501 gggtcggtaa atctggtacg ccgcgacctg aatatggaag cggttgtcgc accagagatt
    19561 tctgcgacgg tgggcgtggc tgcggctttt gcggttaacc ccattgttgg cgcggcagtg
    19621 tttgccgcca gtaaagtgct ggggccgctg tggagcaaag tctccatttt gcgctatcac
    19681 atttcgggtc cgctggacga tccgcaaatc aacgaagtgt tgcgccaacc gcgtaaagaa
    19741 aaagcgcaat gatttgacga gggcgcgtaa ttgccccaat ctcatatgat aatcgttgcc
    19801 aaaggccaac gagccggaac ataactgtag gccggatagg gcgttcacgc cgcatccggc
    19861 agccgtaaaa aatcctctac tgcagtaact aacgagtagc aaaaacgatg agtctgaacc
    19921 tggtaagtga acaattgcta gcggcgaacg gcctgaaaca tcaggacttg ttcgcgatcc
    19981 tcggtcaact ggccgaacgt cgccttgatt atggcgatct ctattttcag tcgagctatc
    20041 acgaatcctg ggttttagaa gaccgcatta ttaaagatgg ttcttacaac atcgatcagg
    20101 gcgttggcgt gcgtgcaatc agcggtgaaa aaaccggatt tgcttacgct gaccaaatca
    20161 gcctgctggc gctggaacag agtgcgcaag cggcgcgcac catcgtccgt gatagtggtg
    20221 atggcaaagt acagacgctg ggcgcggtag agcatagccc gttgtatacc tcggtagatc
    20281 cgctgcaaag catgagccgt gaagagaagc tggatatcct gcgtcgcgtc gataaggttg
    20341 ctcgcgaagc ggacaagcgc gtacaggaag tgactgccag cctcagtggt gtctatgaat
    20401 taattctggt tgcggccacc gacggcacgc tagcggcgga tgtccgtccg ctggtgcgtc
    20461 tttccgtgag cgttctggtc gaagaagatg gcaaacgcga acgcggtgcc agtggcggcg
    20521 gcggtcgttt tggttatgag ttcttccttg ccgatcttga cggcgaagtc cgtgcggatg
    20581 catgggcaaa agaagcagtg cgtatggcgc tggtcaatct ttctgccgtt gctgcaccag
    20641 cgggcaccat gccggtagta cttggcgcag gttggccggg cgtgctgttg catgaagcgg
    20701 taggtcacgg tctggaaggc gacttcaacc gccgtggcac ttcagtattt agtggacagg
    20761 tcggggagct ggtggcttca gaactgtgta ccgtggttga tgacggcacg atggtcgatc
    20821 gccgtggttc ggtggcgatt gatgatgaag gtacgccagg ccagtacaac gtgctgattg
    20881 agaacggcat tctgaaaggc tacatgcagg ataaactcaa cgcgcgtttg atggggatga
    20941 cgccgactgg caacggtcgc cgtgaatcct acgcccatct gcccatgccg cgtatgacca
    21001 acacctatat gctgccgggt aaatcgaccc cgcaagaaat tattgaatcc gttgagtacg
    21061 gtatctatgc accgaacttt ggtggcggtc aggtggatat cacctccggc aaattcgttt
    21121 tctccacttc agaagcatat ctgattgaaa acggtaaagt aacgaagccg gtgaaaggcg
    21181 caacgttgat tggttccggt atcgaaacca tgcagcagat ttcgatggtt ggcaacgacc
    21241 tgaaactgga taacggcgtg ggtgtctgcg gtaaagaagg gcaaagtttg ccggttggcg
    21301 tgggccagcc aacgttgaaa gtcgataacc tgactgttgg cggtactgcg taataatttc
    21361 attattttca gaaggataat taatctttct acgtgcacga acggtcccct cgctcctctg
    21421 gggttagggt gaggggaacc cgttggcaca ggtttgtaga acgtaacagt acaatatgaa
    21481 ttacttctct ttcccgcgcc cgtgcatctc ctgaaacaat ttaccgacct caacaaaata
    21541 atccgtcagc gagttgatca cgacctgtac cttcagcggc agcttatctt tttcggtata
    21601 taacgcataa accgggcgtg gatctgactg ataacgcggc agcaggatct ccagctcccc
    21661 acgattgatc tcgttgatca cccacatcag cggcacgtag gcgatcccgg cacccgccgt
    21721 tagccagcgc accagcgtca tcggatcatt agtcacaaat ctcccttgcg ggatcaggcg
    21781 agtcgagatc ccttccggtg caatcagttc aaattcattg tcgggccgca cgctgtattc
    21841 aagccatgaa tggctactta aatcggcggg tttttccggt atgccgtatt gtgtgagata
    21901 gcttttcgcg gcgcacacca ccattggcat cgcgccaaga cggcgggaaa acaggctgga
    21961 atcctgcaac gcgccgacgc ggatcaccac atccagaccg tcggcaatca ggtcgggggc
    22021 tggaattccg gtaaccagat tggcgctcaa acctgggtat tctttcagca ttttggctgt
    22081 cagcccggcg agaacatttt gtgccatagt tgaagaacag ccaatgcgta gcgtcccgat
    22141 gggggtgtta ttgaaggcat acagttgctc atgaacatcc tgcacttcat gaagcatacg
    22201 acggcagccc tggtagtaaa ttctaccggc ttcggtcagg ccaatgctgc gtgtgctacg
    22261 gtttaacagc tttacctgca actcatcttc cagttttgac accgtctgac tgatggacga
    22321 aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag ccaaattcaa ctactttggc
    22381 aaacaccgac atgcgtttta gtcgttccat tattcactct gacttaaaag tgatttagat
    22441 cacataatat agataacagc ataacagtta cgctaatata ttaaatatca atctacagca
    22501 atgttgctct cgcccggctt tccatgccct tctctgcggc gacagatgct gaaaataata
    22561 acgcctgctc tctctataca accaaggtca acatgagtct gtttcccgtt atcgtggtgt
    22621 ttgggctgtc cttcccaccg atattttttg aattgctttt atcactggcg attttctggc
    22681 tggtgcgccg ggtacttgtg ccaacaggta tctacgactt tgtctggcat ccggcgttgt
    22741 tcaacaccgc gctctattgc tgcttgtttt atttgatatc gcgactgttc gtttgaggtt
    22801 gaagtgaaaa cactaataag aaaattctcc cgtacggcca tcacggtcgt attagtcatt
    22861 ctggccttca tcgcaatttt taatgcctgg gtctattaca ccgaatcccc ctggacgcgt
    22921 gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg acgtttctgg actcattacc
    22981 caggtgaatg ttcatgataa ccagctggtg aaaaaaggac agatactgtt caccatcgac
    23041 cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg atgttgctta ttatcaggta
    23101 ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc gtctcggtgt gcaggcgatg
    23161 tctcgcgaag agatcgacca ggctaacaac gtactacaaa cggttctgca tcagttagcg
    23221 aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg aacgcacggt gatccgagcg
    23281 ccagcagatg gctgggtgac caacctcaac gtctataccg gtgagtttat tactcgagga
    23341 tcaacggcgg ttgcgctggt gaaacagaac tccttctatg tactggccta tatggaagaa
    23401 actaagctgg aaggggtgcg tccggggtat cgtgcagaga tcacgccgct tggcagtaac
    23461 aaagtgctga aagggactgt tgatagtgtt gccgctgggg tcaccaacgc cagcagcacg
    23521 cgtgacgaca aagggatggc gaccatcgac tctgaccttg aatgggtgcg tttggcgcaa
    23581 cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga acatctggcc tgcgggcacc
    23641 actgctacag tggtggtcac tggcaaacaa gatcgcgacg aaagccagga ttcgttcttc
    23701 cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac gatgggtatt ttctccattg
    23761 ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc cattgtactg gcgctgtttg
    23821 ttggctttca cttccagctg gaaacgccac gctgggcggt actgacagcg gcgattgttg
    23881 ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc tggcgctatt cgctatcgtg
    23941 gctttttgcg catcatcggc acatttattg gctgtattgc cggactggtg atcatcattg
    24001 cgatgatccg cgcaccatta ttgatgattc tggtgtgctg tatctgggcc ggtttttgta
    24061 cctggatatc ctcgctggta cgaatagaaa actcgtacgc gtgggggctg gccggttata
    24121 ccgcgctgat cattgtgatc accattcagc cggaaccatt gcttacgccg cagtttgccg
    24181 tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc gattatggcg gatttgctct
    24241 tttctccgcg atcgatcaaa caagaagtag atcgagagct ggaaagtttg ctggtcgcgc
    24301 aatatcaatt aatgcaactc tgtatcaagc atggcgatgg tgaagttgtc gataaagcct
    24361 ggggcgacct ggtgcgacgc accacggcac tacaaggcat gcgcagcaac ctgaatatgg
    24421 aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc gatcaatacg ctatcgctga
    24481 cgctgattac ccaatcctgc gaaacttatc ttattcagaa tacgcgcccg gaattgatca
    24541 ctgatacttt ccgcgaattt tttgacacgc cggtagaaac cgcgcaggac gtccacaagc
    24601 agctcaaacg cctgcggaga gttatcgcct ggactgggga acgggaaacg cctgtcacca
    24661 tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct caagcgcggc gttatcagta
    24721 acacaaaaat caacgccacc gaagaagaga tcctgcaagg cgaaccggaa gtaaaagtag
    24781 agtcagccga acgtcatcat gcaatggtta acttctggcg aaccacactt tcctgcattc
    24841 tgggcacgct tttctggctg tggacgggct ggacttccgg cagtggtgca atggtgatga
    24901 ttgcggtcgt gacgtcactg gcaatgcgtt tgccgaatcc acgcatggtg gcgatcgact
    24961 ttatctatgg gacgctggcc gcgctgccgt tagggctgct ctactttttg gtgattatcc
    25021 ctaataccca acagagcatg ttgctgctgt gtattagcct ggcagtgctg ggattcttcc
    25081 tcggtataga agtacagaaa cggcgactgg gctcgatggg ggcactggcc agcaccataa
    25141 atattatcgt gctggataac ccgatgactt tccatttcag tcagtttctc gacagcgcat
    25201 taggacaaat cgtcggctgt gtgctcgcgt tcaccgttat tttgctggtg cgggataaat
    25261 cgcgcgacag gactggacgt gtactgctta atcagtttgt ttctgccgct gtttccgcga
    25321 tgactaccaa tgtggcacgt cgtaaagaga accacctccc ggcactttat cagcagctgt
    25381 ttttgctgat gaataagttc ccaggggatt tgccgaaatt tcgcctggcg ctgacgatga
    25441 ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt taacgaggat ttatcggcgt
    25501 ttcaccgaca aatgcgccgc acagcagacc atgtgatatc tgcccgtagc gatgataaac
    25561 gtcgtcggta ctttggccag ttgctggaag aactggaaat ctaccaggaa aagctacgca
    25621 tctggcaagc gccaccgcag gtgacggaac cggttcatcg gctggcgggg atgctccata
    25681 agtatcaaca tgcgttgacc gatagttaag tcaaaaccga cgccaaaagc gtcggttttt
    25741 tcatggctat acttagcgat gcacggcaga actcgccgcg aaacgtgacg gtggcaacag
    25801 atgaatattt atgcctttga ttttgatgag attgagagtc aggaggattt ttatcgtgac
    25861 tttagccaaa cctttggtct ggctaaagat aaggtacgcg atctcgactc actatgggat
    25921 gtgttaatga acgatgtcct gccgctacca cttgagattg aatttgttca tctgggagag
    25981 aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg atgaggcaga ggaagagctg
    26041 gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa aaaagcccac gaaccggggg
    26101 caatatcgtc ggacaaaacg atgagggttt atttgtacag ttcagccgta gcgtgccagg
    26161 tgtcaccgct acgagcttca gtaatctggt aggccgttgc gcctttctct tccgcttttt
    26221 tgttcagcat ttcacgtata tccattggcg aagacgccac accacttacg gatacggtcc
    26281 cgattgcttc acgattttgt gcttgtgcag catcaatgga gtcggcagcg aatgcaccga
    26341 aagagagaac agaaagtacg cttaatgcag ctacagtggt tttgattttc atgattttta
    26401 cctcgacata atcttttagc tgggcctttg tttcgtgacc catatcacaa aatcaagtat
    26461 acactaatta ttgaactaat taataccaca ctaactgttt cagttgatac taagtgttaa
    26521 aaatgtgaat tttaataaca aaaaagtata aaaaacacgg ttatttatta ttaattatag
    26581 tattttcaat tagataatga attttgcata atgtgctttt tttaccgtat gtattcattt
    26641 tgtgaatgat gtgtcgcggt aaaacgcact attcgttaaa tgtatcgcag gagaaagaag
    26701 gggaatgaga gggataagca acattttccc cgccgccaga agcgacgggg cagagattaa
    26761 agctcctggt cgaacagctc taaaatcgct tcgtacaggt ctttgacggt gaaaccgtta
    26821 gcaggggtgg taaagatggt gtcatcgcca gcgatggtgc ccagaatacc ttctgctttg
    26881 cccagtgagt ccagcaggcg agcaattaac tgcgccgcgc cagggctggt atgaatcacg
    26941 acaactgcat cgttgtagtc gatatccagc accagattct tcaatggact ggaggtggtt
    27001 ggtacaccca gttcagctgg caggcagtaa accatttcca ttttggcatt gcgtgtacgt
    27061 acagcaccaa acttggtcaa catccgcgag actttagact gattaatatt gtcaaagcct
    27121 tgctcctgca acgcggcgac gatttcgccc tgggagctaa atttctcttc tttaagtaat
    27181 gctttaaatg ctttaactag ttcttcttgc ttagccgagc ttcgcataag tcacccgata
    27241 tggtggttga tacaacatta ttgtgcatac agatgaattt ttatgcaaac agtcagccct
    27301 gaagaaggct gaaataatgt tatgaaagag cgggatttta tcaaatttcg ttattgagaa
    27361 acatgcctgc gtcacggcat gcaaattctg tttaaaagta aattaattgt tatcaaattg
    27421 atgttgtttt ggctgaacgg tagggtatat tgtcaccacc tgttggaatg ttgcgctaat
    27481 gcataagcga ctgttaatta cgtaagttag gttcctgatt acggcaatta aatgcataaa
    27541 cgctaaactt gcgtgactac acattcttga gatgtggtca ttgtaaacgg caattttgtg
    27601 gattaaggtc gcggcagcgg agcaacatat cttagtttat caatataata aggagtttag
    27661 gatgaaagtc gcagtcctcg gcgctgctgg cggtattggc caggcgcttg cactactgtt
    27721 aaaaacccaa ctgccttcag gttcagaact ctctctgtat gatatcgctc cagtgactcc
    27781 cggtgtggcc gtcgatctga gccatatccc tactgctgtg aaaatcaaag gtttttctgg
    27841 tgaagatgcg actccggcgc tggaaggcgc agatgtcgtt cttatctctg caggcgtagc
    27901 gcgtaaaccg ggtatggatc gttccgacct gtttaacgtt aacgccggca tcgtgaaaaa
    27961 cctggtacag caagttgcga aaaactgccc gaaagcgtgc attggtatta tcactaaccc
    28021 ggttaacacc acagttgcaa ttgctgctga agtgctgaaa aaagccggtg tttatgacaa
    28081 aaacaaactg ttcggcgtta ccacgctgga tatcattcgt tccaacacct ttgttgcgga
    28141 actgaaaggc aaacagccag gcgaagttga agtgccggtt attggcggtc actctggtgt
    28201 taccattctg ccgctgctgt cacaggttcc tggcgttagt tttaccgagc aggaagtggc
    28261 tgatctgacc aaacgtatcc agaacgcggg tactgaggtg gttgaagcga aagccggtgg
    28321 cgggtctgca accctgtcta tgggccaggc agctgcacgt tttggtctgt ctctggttcg
    28381 tgcactgcag ggcgaacaag gcgttgtcga atgtgcctac gttgaaggcg acggtcagta
    28441 cgcccgtttc ttctctcaac cgctgctgct gggtaaaaac ggcgtggaag agcgtaaatc
    28501 tatcggtacc ctgagcgcat ttgaacagaa cgcgctggaa ggtatgctgg atacgctcaa
    28561 gaaagatatc gccctgggca aagagttcgt taataagtaa ttgattagcg aataataaaa
    28621 aaccggagca cagactccgg tttttgtttt gagcactcga cttaattggt tgccggatat
    28681 tcctgaatgg tgacctgcag cgttaactgc ttatcatcac gcatcactac aaccgggatc
    28741 accgaaccag ggcgaatttc cgccacctga tccatcgtct ccagagcaga gatggccggt
    28801 ttgttatcca ccgaaataat cagatcgttg acctgaatac ccgcattcgc cgctgggccg
    28861 tcaggtgaca cttcattaac cacgatccct tgcagttgat ctataccacc gctctgcgcg
    28921 tgcagtggtg cgatctcgcg tccgccgata ccaatgtagc cgcggatcac gcgaccatcg
    28981 cggatcagct tatccataat tttggttgct aactggaaag gaatcgcaaa gccgatacct
    29041 tccggcgttt cgccatcgtt actcttatca aacgacagcg tgttaatgcc catcagttct
    29101 cccagcgagt tcaccagcgc gccgccagag ttaccgtggt taatggaagc atcggtttgt
    29161 aggaagtttt gccgcccggt cgggttcaga ccgattcgac ccgtggcact aataatccct
    29221 tgggtaatgg tctgcccgag gttgtacggg ttaccgatcg ccagtactac gtcgccaatg
    29281 tgcggtacgc gacgtgcatt aattggaatg gtaggtaaac cgccagtggc attaatttta
    29341 agtaccgcca gatcggttag agagtcagat cccaccagca atgcttcaaa tacacgtcca
    29401 tcctgtaagg cgacgatgat ctgatcggcg tcgttgatga cgtgtttatt ggtgatgata
    29461 taaccgcgtt gatccatgat tacaccggat cccagggtgc ggatctcaag ctggttgtga
    29521 gagttggtgt tcaaaccacg gttgtaaacg ttaaccaccg ctggcgcggc gcggcgaacc
    29581 gccagattat agctggcagg cgtctcatcg gtactgtcaa attgcggagt ggaaagcggg
    29641 ttaaggctgc gcagcgaagg catggcaacc agcagaatag cgccgacaat taatccaatc
    29701 gcaacggaac gtaagagctt cacaaacatg atggaggcgt cattaaaaaa gggaacggca
    29761 gcagcatacc acgagttaac cggacatcac acgtcagcct gatgcccggt ttacgacatt
    29821 aacgcatcag cagatagatg ctttcattgc cgcgtacaat ttgcagggcg atgatggccg
    29881 gttttgccgc cagcacttta cgcatttcag caatcgagtt cacccgatcg cggttgacgc
    29941 caatgatcac atcgtctttt tgcaagccag cctgagcagc tgggcttcct ttgacaactt
    30001 catctatctt aatacctttg ccgccatctt ttagctgacc atcgctcaac gttgcacctt
    30061 ccagcgctgg ccgacgaaga ggtgctggta tcaagcgtca cttctacttc cagtggtttg
    30121 ccgttacgca gcaggccaag cttcactttc gtgcccggct ctgtagtcgc gatacgagag
    30181 cgcaactcag caaagctatt cagcggtttg ccgttgaggc tggtaataat atcgcccgct
    30241 ttgacgcccg ctttcgctga gccagaactt ggcaacactt cgctgacaaa cgcgccacgc
    30301 tgcacgtcaa ggttgaaggc tttggcgata tcggcactca tctcggtgcc tttgatgcct
    30361 aacaaaccgc gtttgatttc accaaagtcg ataagctgct gcgccagtgt tcgcgccata
    30421 ttactgggga tggcaaatcc aatcccgacg ctcccgccgc caggcgcaag gattgcagtg
    30481 ttgatgccaa ttaactcacc gttaaggttt aatagtgcac cgccggagtt accgcggtta
    30541 atggaagcat ctgtctggat aaagttttcc agaccttcaa gattcaaccc gctgcggcct
    30601 aatgcggaaa caatgccaga ggtggcggtt tgcccaaggc caaatgggtt accgaccgct
    30661 acggcaaaat caccgacgcg caatttatcg gagtcggcaa tagcgatttg cgttaatttg
    30721 ctcgggtttt gaatttgtaa cagggcgata tcgctctggt catcgctacc aatcagtttt
    30781 gcatcaaact cgcgcccatc attgagctga atactgattt tctgtgcctg attaatcaca
    30841 tggttgttgg tcagcacata gcctttactg gcgttgatga tgacaccgga acctaaacct
    30901 tcgaagggtt gtgcaggttg atccggtaaa tcatcaccaa aaaacttttt gaattcttcc
    30961 gggattttct gtccctgact ggccgttcct tccacccgta cgctcaccac tgccggaagc
    31021 actttttcca gcattggagc cagactgggg agaggggcct gatcggcaac ctggcctgga
    31081 atcgacgcga cggcctgaaa tgacgccgag agagttaacc cggcacttaa cgctaatgca
    31141 ctcaacagct gggtttgttt tttcattatt cctgctctcg tacctgaatg ataagaaaag
    31201 agattcaaaa cagtttgatg ttattgaatt ttcagacggc taacaatgag acaccagatt
    31261 aaatgatagt cggggaggga gagaagagga gggcgcaatg gctgcgcccg aaaaataaat
    31321 tagtcgcgct tcgcgccagt acgcagcagg ccggatgcgc cttcagaata gtcgcgaggc
    31381 acctgcaccg gtgcctgatc gttgctggct tcagactctg ccagacgatt acggaacggg
    31441 tttgcttcag cagacagttc cggcagcagg ctgctggagc tttttgccat gtgctgatac
    31501 agctggcgat agtcgtgcgc catggtatcc agtaattccg cgctgcgggc aaagtggcta
    31561 accagctctt cgcgatactc gtccagttca gctttattct tttccagttc gtactgcaac
    31621 gcctgttgct ggcgtagttt acgattacca aaacgcatgg ccacagcacc aataatgatg
    31681 ccgacgacta acccaattag cgcatattcc caggtcatga acatctcccg ttgtcttttg
    31741 tttccgtagg gtgttggctt caggctccat gcctgcggct gattatgcca ctataaccgt
    31801 taattccaca gaagtggaat cccgactgca tatcgcgtag tgtagaacgg cctttttttc
    31861 gtcaaccgtg aacaacggcg caccgattat tcaaggaata acaataagat catgcaaagc
    31921 gttaccccaa catcgcaata cctgaaggcg ctcaatgaag gcagccatca acccgacgac
    31981 gttcaaaaag aggccgtcag ccgcctggaa attatttatc aagaactcat caatagcagg
    32041 ccaccagccc ccaggacgag tgggctaatg gcgcgggtcg gtaagctgtg gggtaaacgc
    32101 gaagacacaa agcatacgcc agtgcgtggc ttatatatgt ggggcggtgt aggacgcggg
    32161 aaaacctggc tgatggacct tttctatcaa agcctgccgg gagagcggaa acagcgcctg
    32221 cactttcacc gttttatgct gcgggtgcac gaagagctaa ctgccttaca ggggcagacc
    32281 gatccgctgg aaattattgc cgatcgcttt aaagccgaaa ctgacgtgct ctgttttgac
    32341 gaattttttg tttctgatat taccgacgcc atgctacttg gcggtctgat gaaagccctg
    32401 ttcacccgcg gtattaccct ggtagcgacg tcaaatattc cgccggacga actttatcga
    32461 aatggcctgc aacgtgcgcg ttttctgcct gcaatcgatg ccattaaaca gcattgtgat
    32521 gtaatgaacg tggacgctgg tgttgattat cgactgcgta cactcactca ggcgcatctg
    32581 tggctttcgc cactcaacga tgaaacccgg gcgcagatgg ataaactatg gttggcgctg
    32641 gcgggggtga aacgagaaaa ttcaccgacg ttagaaatca accatcggcc attggcgaca
    32701 atgggcgtcg agaaccagac gctggcggtt tcttttacta cgctgtgcgt cgacgcccgc
    32761 agtcagcatg actatattgc gctctcacgc ctgtttcaca cagtaatgtt gtttgatgtg
    32821 ccagtcatga cgcggttgat ggagagcgaa gcgcggcgct ttattgcgct ggtggatgag
    32881 ttttacgagc gccatgtcaa attagtggtg agtgcagaag tgccgctgta tgaaatttat
    32941 cagggtgagc ggctgaagtt tgagttccag cgttgcctgt cacgtctgca agagatgcaa
    33001 agcgaagagt atctgaagcg cgagcatttg gcgggttaaa acctgccaca aatcacaaaa
    33061 aggggtcgat ctttgacccc gacttctcta taatcctgcg accccacgtt acaagaaagt
    33121 ttttttccca aaactttttg tgtgctggca taggctattc gaaggggtag gtttgccgga
    33181 ctttgtcgtg tgaacctcaa caattgaaga cgtttgggtg ttcaccaacg tgtaactatt
    33241 tattgggtaa gcttttaatg aaaactttta cagctaaacc agaaaccgta aaacgcgact
    33301 ggtatgttgt tgacgcgacc ggtaaaactc tgggccgtct ggctactgaa ctggctcgtc
    33361 gcctgcgcgg taagcacaaa gcggaatata ctccgcacgt agataccggt gattacatca
    33421 tcgttctgaa cgctgacaaa gttgctgtaa ccggcaacaa gcgtactgac aaagtgtact
    33481 atcaccacac cggccacatc ggtggtatca aacaagcgac ctttgaagag atgattgctc
    33541 gccgtcctga gcgtgtgatt gaaatcgcgg ttaaaggcat gttgccaaaa ggcccgctgg
    33601 gtcgtgctat gttccgtaaa ctgaaagttt acgcgggtaa cgagcacaac cacgcggcac
    33661 agcaaccgca agttcttgac atctaatcgg gattataggc aatggctgaa aatcaatact
    33721 acggcactgg tcgccgcaaa agttccgcag ctcgcgtttt catcaaaccg ggcaacggta
    33781 aaatcgtaat caaccaacgt tctctggaac agtacttcgg tcgtgaaact gcccgcatgg
    33841 tagttcgtca gccgctggaa ctggtcgaca tggttgagaa actggacctg tacatcaccg
    33901 ttaaaggtgg tggtatctct ggtcaggctg gtgcgatccg tcacggtatc acccgcgctc
    33961 tgatggaata cgacgagtcc ctgcgttctg aactgcgtaa agctggcttc gttactcgtg
    34021 acgctcgtca ggttgaacgt aagaaagtcg gtctgcgtaa agcacgtcgt cgtccgcagt
    34081 tctccaaacg ttaattggct tctgctacgg cagaaaacaa tttgcgaaaa aacccgcttc
    34141 ggcgggtttt tttatggcta aattctgaat cagcgtaaaa aatgggaagt tgctttttgc
    34201 tgccgtctga aagacaggta aaacaaaaac cacatcgcca ataagggtct aagtcaacta
    34261 tttcagacta aagcgcatct ctttttcccc atttccggca tccactcacc acaaaggtcg
    34321 caaaatctgg taaactatca tccaattttc tgcccaaatg tcgggtattg ctcatttttt
    34381 gtttgatttt cgaacaaaga gagtcgttcc ttattgggta acacaacttc tgactggcca
    34441 cctggtggct ggtagcagta aaaattctga ctatacctgg aggttttcat ggctgtcgct
    34501 gccaacaaac gttcggtaat gacgctgttt tccggtccta ctgacatcta tagccatcag
    34561 gtccgcattg tgctggctga gaaaggtgta agtttcgaga tcgaacacgt ggaaaaggac
    34621 aatccgcctc aggatctgat tgacctcaac ccgaatcaga gcgttccgac cctggtggat
    34681 cgtgagctga ccctgtggga atctcgcatc attatggaat atctggatga gcgtttcccg
    34741 catccgccac tgatgcctgt ttacccggta gctcgcggtg aaagccgtct gtacatgcat
    34801 cgcatcgaaa aagactggta cacgctgatg aacactatca tcaacggttc agcttctgaa
    34861 gcagatgccg cacgtaagca actgcgcgaa gaactgctgg cgattgcgcc ggtcttcggt
    34921 cagaagccgt acttcctgag cgatgagttc agcctggtcg attgctacct tgctccgctg
    34981 ctgtggcgtc tgccgcaact gggcatcgag ttcagcggcc cgggtgcgaa agagctgaaa
    35041 ggctatatga cccgcgtctt tgagcgtgac tctttccttg cttctttaac tgaagcagaa
    35101 cgtgaaatgc gtctgggccg gagttaatct gtatggattt gtcacagcta acaccacgtc
    35161 gtccctatct gctgcgtgca ttctatgagt ggttgctgga taaccagctc acgccgcacc
    35221 tggtggtgga tgtgacgctc cctggcgtgc aggttcctat ggaatatgcg cgtgacgggc
    35281 aaatcgtact caacattgcg ccgcgtgctg tcggcaatct ggaactggcg aatgatgagg
    35341 tgcgctttaa cgcgcgcttt ggtggcattc cgcgtcaggt ttctgtgccg ctggctgccg
    35401 tgctggctat ctacgcccgt gaaaatggcg caggcacgat gtttgagcct gaagctgcct
    35461 acgatgaaga taccagcatc atgaatgatg aagaggcatc ggcagacaac gaaaccgtta
    35521 tgtcggttat tgatggcgac aagccagatc acgatgatga cactcatcct gacgatgaac
    35581 ctccgcagcc accacgcggt ggtcgaccgg cattacgcgt tgtgaagtaa tacaaaacag
    35641 gcccaggcgg cctgttttgt ctttttaatg acttaataaa atagcgtgat tgtggcaatc
    35701 atattcacca cgaaaccgac tgccatgggg atcgctgtcc gcttcaccac cgcaaaaggc
    35761 gaaacgcctg caattcctga cgtaacgacc accaccgcag taatcggcga aaccgcgcgc
    35821 gctagcgtcg tggcaaaatg catcggcata atcattacaa ccgctggtac atgtagtccg
    35881 gctgcgatat tcggaataag actggcaaat gacataaacg gcgcattgcc agagcccatc
    35941 acaatggcac aaatggcaat gactagcgcc ataataatca tcacgccaat accgcccaga
    36001 ccagaatgct ccgcacccct gataaccgca tcaacagtgc caatcgtcgt taagcctttc
    36061 gcaaatattt cacccgcgac caccagcgtt accacgttga caaactgcgt acccatgccg
    36121 tcaaaaaacg cctgcacatc gtccattgtt tcgcgcaagt tatgcttgcg gaagaactca
    36181 acaaacatcg tcaccgtcaa acttagtaac atcaccacca ccagatgcag ttctgactgc
    36241 atcagcccga cgtgggcgag gaacagcgag ccgagcatca ggattaacgg catcacaggt
    36301 aaaatggcgt aatagagcgg cgggacatta tcgagagttt tttgctctgc ctgttcgtga
    36361 ttgatatttt tatctttttt gtcaaaagcg cgttgcacga aaaagtggga gatcgccacc
    36421 gagataatga cgcaagaggc gaccggaagc tggtagtgga agaagtatgt ggcaattttc
    36481 attcccgcga cctgggctgc aaaaatggag ttcgtttcca gaatccccca ctcaatggac
    36541 atcgtggttg cgataaccgc caccgcagag aggcgactta ctcccagact caccagcgtc
    36601 ggaaataagg tgaccatcag caacataccc agaccggagg cgctggtaat aaactgcgcc
    36661 atgatttggc cgatgacgta agttgccgac agaataatat acggcgagcg aatgagtttt
    36721 aacgggcggc ttagcaagct caccatcgcg caactggctc ctatgcgctc catgtagcgg
    36781 gcataaccgc ccaccgccat aatcgacagc cccaatccgg caatgcggtt ggacagcatg
    36841 cgcaggattt cattataaat gtcgacgatc aagtagccgc tgcttttggc gggatcgagt
    36901 acaccaccga accctaacca gactccgcac
//
LOCUS       DAJTUP010000030        36461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTUP010000030 DAJTUP010000000
VERSION     DAJTUP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.030"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(20..1255)
                     /locus_tag="OO468_001558"
     CDS             complement(20..1255)
                     /locus_tag="OO468_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6258718.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHRQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1402..1478)
                     /locus_tag="OO468_001559"
     tRNA            complement(1402..1478)
                     /locus_tag="OO468_001559"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1442..1444),aa:Pro,seq:tgg)
     gene            complement(1521..1607)
                     /locus_tag="OO468_001560"
     tRNA            complement(1521..1607)
                     /locus_tag="OO468_001560"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1571..1573),aa:Leu,seq:cag)
     gene            complement(1628..1703)
                     /locus_tag="OO468_001561"
     tRNA            complement(1628..1703)
                     /locus_tag="OO468_001561"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1668..1670),aa:His,seq:gtg)
     gene            complement(1762..1838)
                     /locus_tag="OO468_001562"
     tRNA            complement(1762..1838)
                     /locus_tag="OO468_001562"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1802..1804),aa:Arg,seq:ccg)
     gene            complement(1941..3326)
                     /locus_tag="OO468_001563"
     CDS             complement(1941..3326)
                     /locus_tag="OO468_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6258719.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3517..4257)
                     /gene="rffM"
                     /locus_tag="OO468_001564"
     CDS             complement(3517..4257)
                     /gene="rffM"
                     /locus_tag="OO468_001564"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR6258720.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4260..5612)
                     /gene="wzyE"
                     /locus_tag="OO468_001565"
     CDS             complement(4260..5612)
                     /gene="wzyE"
                     /locus_tag="OO468_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR6258721.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5609..6688)
                     /gene="rffT"
                     /locus_tag="OO468_001566"
                     /gene_synonym="wecF"
     CDS             complement(5609..6688)
                     /gene="rffT"
                     /locus_tag="OO468_001566"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR6258722.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6685..7935)
                     /gene="wzxE"
                     /locus_tag="OO468_001567"
     CDS             complement(6685..7935)
                     /gene="wzxE"
                     /locus_tag="OO468_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR6258723.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(7937..9067)
                     /gene="rffA"
                     /locus_tag="OO468_001568"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(7937..9067)
                     /gene="rffA"
                     /locus_tag="OO468_001568"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR6258724.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9072..9746)
                     /gene="rffC"
                     /locus_tag="OO468_001569"
                     /gene_synonym="wecD"
     CDS             complement(9072..9746)
                     /gene="rffC"
                     /locus_tag="OO468_001569"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR6258725.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9724..10605)
                     /gene="rfbA"
                     /locus_tag="OO468_001570"
     CDS             complement(9724..10605)
                     /gene="rfbA"
                     /locus_tag="OO468_001570"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR6258726.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10624..11691)
                     /gene="rffG"
                     /locus_tag="OO468_001571"
     CDS             complement(10624..11691)
                     /gene="rffG"
                     /locus_tag="OO468_001571"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR6258727.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11691..12953)
                     /gene="wecC"
                     /locus_tag="OO468_001572"
     CDS             complement(11691..12953)
                     /gene="wecC"
                     /locus_tag="OO468_001572"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR6258728.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(12950..14080)
                     /gene="wecB"
                     /locus_tag="OO468_001573"
     CDS             complement(12950..14080)
                     /gene="wecB"
                     /locus_tag="OO468_001573"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR6258729.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14136..15182)
                     /gene="wzzE"
                     /locus_tag="OO468_001574"
     CDS             complement(14136..15182)
                     /gene="wzzE"
                     /locus_tag="OO468_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR6258730.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15194..16297)
                     /gene="wecA"
                     /locus_tag="OO468_001575"
     CDS             complement(15194..16297)
                     /gene="wecA"
                     /locus_tag="OO468_001575"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR6258731.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16537..17796)
                     /gene="rho"
                     /locus_tag="OO468_001576"
     CDS             complement(16537..17796)
                     /gene="rho"
                     /locus_tag="OO468_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR6258732.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17881..17982)
                     /gene="rhoL"
                     /locus_tag="OO468_001577"
     CDS             complement(17881..17982)
                     /gene="rhoL"
                     /locus_tag="OO468_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR6258733.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18123..18452)
                     /gene="trxA"
                     /locus_tag="OO468_001578"
     CDS             complement(18123..18452)
                     /gene="trxA"
                     /locus_tag="OO468_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR6258734.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18583..19848
                     /gene="rhlB"
                     /locus_tag="OO468_001579"
     CDS             18583..19848
                     /gene="rhlB"
                     /locus_tag="OO468_001579"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR6258735.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            19982..21466
                     /gene="gppA"
                     /locus_tag="OO468_001580"
     CDS             19982..21466
                     /gene="gppA"
                     /locus_tag="OO468_001580"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR6258736.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21513..23534)
                     /gene="rep"
                     /locus_tag="OO468_001581"
     CDS             complement(21513..23534)
                     /gene="rep"
                     /locus_tag="OO468_001581"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR6258737.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23621..23902
                     /gene="ppiC"
                     /locus_tag="OO468_001582"
     CDS             23621..23902
                     /gene="ppiC"
                     /locus_tag="OO468_001582"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR6258738.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(23948..25423)
                     /gene="ilvC"
                     /locus_tag="OO468_001583"
     CDS             complement(23948..25423)
                     /gene="ilvC"
                     /locus_tag="OO468_001583"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR6258739.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25573..26463
                     /gene="ilvY"
                     /locus_tag="OO468_001584"
     CDS             25573..26463
                     /gene="ilvY"
                     /locus_tag="OO468_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR6258740.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26460..28004)
                     /gene="ilvA"
                     /locus_tag="OO468_001585"
     CDS             complement(26460..28004)
                     /gene="ilvA"
                     /locus_tag="OO468_001585"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR6258741.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28007..29857)
                     /gene="ilvD"
                     /locus_tag="OO468_001586"
     CDS             complement(28007..29857)
                     /gene="ilvD"
                     /locus_tag="OO468_001586"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR6258742.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(29922..30851)
                     /gene="ilvE"
                     /locus_tag="OO468_001587"
     CDS             complement(29922..30851)
                     /gene="ilvE"
                     /locus_tag="OO468_001587"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR6258743.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30871..31134)
                     /gene="ilvM"
                     /locus_tag="OO468_001588"
     CDS             complement(30871..31134)
                     /gene="ilvM"
                     /locus_tag="OO468_001588"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR6258744.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31131..32777)
                     /locus_tag="OO468_001589"
     CDS             complement(31131..32777)
                     /locus_tag="OO468_001589"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR6258745.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32780..32830)
                     /locus_tag="OO468_001590"
     CDS             complement(32780..32830)
                     /locus_tag="OO468_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258746.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(32916..33014)
                     /gene="ilvL"
                     /locus_tag="OO468_001591"
     CDS             complement(32916..33014)
                     /gene="ilvL"
                     /locus_tag="OO468_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR6258747.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33366..34845
                     /locus_tag="OO468_001592"
                     /pseudo
     CDS             33366..34845
                     /locus_tag="OO468_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(34870..35208)
                     /gene="maoP"
                     /locus_tag="OO468_001593"
     CDS             complement(34870..35208)
                     /gene="maoP"
                     /locus_tag="OO468_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR6258748.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35327..36166
                     /gene="hdfR"
                     /locus_tag="OO468_001594"
     CDS             35327..36166
                     /gene="hdfR"
                     /locus_tag="OO468_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR6258749.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     DVY"
     gene            complement(36262..36337)
                     /locus_tag="OO468_001595"
     tRNA            complement(36262..36337)
                     /locus_tag="OO468_001595"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36302..36304),aa:Trp,seq:cca)
     gene            complement(36346..36422)
                     /locus_tag="OO468_001596"
     tRNA            complement(36346..36422)
                     /locus_tag="OO468_001596"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36386..36388),aa:Asp,seq:gtc)
BASE COUNT         8903 a         9977 c         9187 g         8394 t
ORIGIN      
        1 cttccaaatg gccgcttttt tacttattca ccaccatcaa atgtgcctgc ataatgtcac
       61 tggccgggcg accgtgcgcc agcaaatcag ccattgcttt aagatatggc ggtagatggc
      121 ggaaataacg ctgatacccg gcacacaaat aattcagccc cggtttgccg ctggcatcga
      181 gcatgaagcg gtgtttcggg cagcctcccc aacatgcttt taacacgtta caactgcgac
      241 actgcgccgg taattgctta aatttatctt caccaaacac ctgctgttgc ggggaatcga
      301 tcatttctgc aattgtttgc cgatgcatat tccccagccg atattgcgga taaacatagt
      361 gatcgcaggc gtaaacgtcg ccgttgtgct caacaatcac cgagcgccca caggttggct
      421 gatgatggca aaccgcaccc ggcgcaccga caaaattggc aaacgcccat tcgatattca
      481 tcacgaaaat cttgccgacg tcgcgtttga tccagtggtc aaaaatcgcc accagaaatt
      541 caccaaattc atcggggcgc acagaccatt ccgtcagttc cccctgaata tcaccaggcg
      601 catgtagttt cagtccatcg cggacagctg tttcatcagc caggcgctcg accaccggaa
      661 taaactggat gaattcgact cccgcatcgc acaaaaaatc ataaacctgt aacggttgct
      721 gcgcgctggt gcggttgacg cagaccagca cgttatagtc gacatgatgt ttttgcagga
      781 gcgtcagggc acgcatcacc agtttatgcg tgggtctgcc gcctttggtc acgcgatatt
      841 gattgtggat ctcagccgga ccatccagcg ataacccaac aagaaaatta ttttctgcca
      901 gaaatgcaca ccattcgtca tcgagcagca cgccgttagt ctggaaactg ttacttatct
      961 tcctgccagc accatatttc gcctgtagcg ccacggcacg gcggtaaaac tccagcccga
     1021 gtagcgttgg ttcgccgccc tgccaggtaa aagccacttc gttttgcagt tcgctggcgg
     1081 cgatatagtg acggatatac gcttccagcg tgtcatcgtc catatgcgtg actggctttt
     1141 cgcggtaaag ggattgtttt tcgagataaa aacagtagtc acagttcaga ttacaatcgg
     1201 aaccgctcgg tttcgctatc acatgaaaag cacgcgttgg aacctgttgc agcatggtcg
     1261 ctccttgcct gaagtcatcc ttcaatacgt taagggcgaa atcataccgt ggggcaatga
     1321 gttattacgg gcgtttattg cgaagggagg gtgacgaaat gcacagaaaa caaaaaaccc
     1381 cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt cgaacctccg acccactggt
     1441 cccaaaccag ttgcgctacc aagctgcgct actcgccgaa atactgcttt ttgaattttt
     1501 agttcaattc tttaaagtcg tggtgcgagg ggggggactt gaacccccac gtccgtaagg
     1561 acactaacac ctgaagctag cgcgtctacc aattccgcca ccttcgcatt gtcacaactt
     1621 ctaataatgg ggtggctaat gggattcgaa cccacgacaa ctggaatcac aatccagggc
     1681 tctaccaact gagctatagc caccactaca aatcttgtta cgcggtatta ctaccaccgc
     1741 agctcaagcg ctgggactaa atggcgcgcc cgacaggatt cgaacctgag acctctgcct
     1801 ccggagggca gcgctctatc cagctgagct acgggcgctt agcgccgttg cgggggtgga
     1861 taatacggac ttcccacccc tctgtctagt ccctttttaa ataaattgct cgtttggtta
     1921 cggtttgtgc gttttgctgc ttattcctcc agtttatgcg ctttaccgtg gcgattaagc
     1981 ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga tgatgccgac aaacagcgac
     2041 atacgcgtat cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa tgccattgtt
     2101 acgtagttgg cccacgggaa caggattgag cggaacggat ggctggcaat cgccgcttta
     2161 tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa accatggcac catccccgga
     2221 agcacgctgg cactgtagac gtagacaaac acacgctgcg gattgggaat gatgtagttc
     2281 aggcatgagc caattagcag aatagcaata gatactgcca cacccgcaac cggtacgccg
     2341 tgacgggaaa cttttgccat tgccgccggt aactgacggt ttttcgccag tgcgtagagc
     2401 atacgtccgc aactgtacat gccgctgtta cagccagaga gcgcagccgt cagcaccaca
     2461 aagttgataa tgcccgccgc tgcggtaata ccgattttgg caaaagtcag tacgaacggg
     2521 ctgccgttgc tgccaatttc attccacggg aagatggtga cgataacgaa aatcgcgcct
     2581 acgtagaaaa tcaggatccg ccacagcact ttgcctacgg cactgcgcag cgtcacctgc
     2641 ggattcttcg cttcaccggc agtaatgcca atcagctcca cgccctggta ggacgccacc
     2701 acaatacaca gagcggtcag gaaccctttc cagccacccg caaagaaacc gccatgctct
     2761 gtgagattgc taaaaccaat cgactgcccg ccattgccaa agccaaagaa aatcacgccc
     2821 aggccaatga caatcatcac gataatcgtg gtgactttga tcatcgcgaa ccagaactcg
     2881 atttcgccgt acaaccgtac cgccgccaga ttcgccaacg ccaccagcgc caccgcgatc
     2941 aatgcgggta tccactgcgc catctccggg gaccaaaact ggacataaac gccaatggcg
     3001 gtgatttctg agatccccac cgccatccac ataaaccagt aagaccaggc ggtgagatag
     3061 ccaaaaaacg ggctcatata acgatgcgca taaacggcga acgaaccggt aaccggttcg
     3121 aggaacaaca tttcgcccat tgaacgcatg atgaaaaaaa cgaacagccc ggcgatgata
     3181 taggccaaca atacggatgg cccggcccat ttcagggtac tggcggcccc cataaacagg
     3241 ccgacgccaa tggtgccccc cagggcgatg agttcgatat gtcgagcttc cagcccacgc
     3301 tgtagctctg gtttgttatc tgccataaat cctcgtgttg tgtttgcatg ctttccggtg
     3361 ttaccggtta tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat tcaggagaaa
     3421 tggcaaataa agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag acaggcgacg
     3481 aagtgaccac tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg tgtagtgcca
     3541 gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg aaagcaggcg
     3601 gtagagccac tccagtccca gcgtttgcca gattttcggt gcgcgtttta cgtgaccggt
     3661 gaaaacatcg taagtcccgc caacgcccat atacagcgca tctggatgca ccagacggca
     3721 gtcgcgcatg aagatctcct gctttggcga tcccatcgca acggtgacga tttgcgctcc
     3781 gctggcatga atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat aaccatcctg
     3841 actgccaacg atattcacat tccattggtt gcgcagttta gcttcagttt gcgccagcac
     3901 ttcaggttta ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc gcgccatcag
     3961 ctcttcccag agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt actttttacg
     4021 tactgaacgt acaatgctga tgccatccgc atatttaaat tcggcagcgt taattaactc
     4081 cctgacctcg gcgttatctt caatggtcag cattttttcg gcattaatgg caaccagcgt
     4141 tccctgctta agctgcccgt cagcaaacag ataatcgagg gcatgctgca tatcacgcca
     4201 accaatcaac tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt tattcattgt
     4261 tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag tccggcgctt
     4321 tcaaaaagcc agtacaacag ttttgcgatc atcagacatg cgccgaagac cacgataaaa
     4381 aagactacgc gtgagacaaa cgaatccagc ccttcacgtg ccagcacgat catattgaag
     4441 atcgccccaa agcagaaact gtgcaatatc gcagccttat agcgattagt ctcgcggttg
     4501 cccagctcat acagccagtc gaaccatttg atgatcagtc caaccacaat cgccccgagt
     4561 gggatgaaca acgcgccgcc catcaccacc agtgagccta taagcgtagg cgagatcgcc
     4621 agtccggagt ggttattcag tacttcccag gtaaagtaat tggctgagtt cagcaccata
     4681 ctcgggcgcc ccggccacag ccaggaaggg ataaagacat agaaatcgcg gacaattgga
     4741 gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag attctcccac
     4801 ggtgaaaagg tgtcgcgagt gagatagaga aacgtataga acgcttcatc gccgctcaca
     4861 ttcattccat agcgttttaa cgccagccag aacatgccaa caatccccag cacgcccgcc
     4921 gccgccagca tccacaacga aatccagccg cgaataatgc caataaacag gaagatagcg
     4981 aatgcgatga tgatattggc gcgagtgccg ccgacaatca tataagtcag caagccaaag
     5041 gcgaccgtgc tgacgaggaa aaacagccac gctttgctgt cctggcgcag aaagtagacc
     5101 accagcatcg ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc ggagacttca
     5161 ctggaaaaga tctgactgta ggagttaagc cggaacagca aaaagccgtt gtgcatgaag
     5221 aagatgccga cgcttaccag cgcgataccc atcaggatca cccacgtaag attggtctcc
     5281 acgcggttca tggtaaacag cggacggcgc ggtacatcag caacgcgttt gcgtaggcgg
     5341 gttttgtagg tgacatagta aaccgcgtag aagcagcccg cagaaagcaa cacctgcaac
     5401 aagatttctg gcggcgcgac accaacatca aagcgaaata ccagcacgct ggtcagcggg
     5461 aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga agacattgaa gttaaagcgc
     5521 acacggcgaa actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag cagccagacc
     5581 acaaacaggc cactgaattg cagcagactc atgcgacctc cccggcggca atcgccaacg
     5641 cccgctgcca gccttgtaga tagttagggc taaagaaggc aatggtgttt ttatccgccg
     5701 acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg gtagtaaaca
     5761 gcactggcaa atgctgttcc gtcatatcct gccagaacgg attttccctg ttaagcacac
     5821 aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg cgggcaaaaa
     5881 taaagtaacc aagatcgcac tgacgaagta gcgccagata ggcgtcaaat tccagttttt
     5941 cgctcaaaat ttgcagattt tcttcgctga ataactccag ccccgcctga cgaacttcct
     6001 caatgtacgc ttcgttatta ggcggatatc ccatcggcac caccactttt accgtatcgc
     6061 caaattgctg atgaacggca cgtaaagcgg cgacatgctc attgctgcgg tcgccggagt
     6121 tccccaccag aatggtcatt tttccttcac gttgccgatc gttcgccatc gtattgagag
     6181 aagggtccat ccgcgtcggg aagtacagca gttctccccg cacctttggg tgtgttttgg
     6241 caaaaaagct caaatcgccg cgggtggcaa atacacagcc gactcgcttt tgcgccaggc
     6301 gacgtagtgg gtaaaaaagc ttatatctca agccactgga aagctcatac aggtctgccc
     6361 cccagatatg ccagaaaaac tggctgggct taatgccacc actcagcaga gccagccaca
     6421 gtgtgggatt gaactgaccg tggaaaaaaa atcgctgctg acggttagct tttgctttcg
     6481 cgatgaccgc ttccgccagc gattttttcc cagggaaaaa ttgcacagaa agcgccggac
     6541 agctatcact taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg tgctcgctcg
     6601 tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg gttatggtga gggatatccg
     6661 atcccagtac gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa acacgccaca
     6721 acaaagagaa aaatagacga tataagttgc catatatgcc tgcgccgcgc ccagtgcacc
     6781 atgcgcaggg attagccagt gggcaaatac catcaataaa gtgaactggc tgacttccgc
     6841 cagaatataa aaccgcagtg acgctttggc gatcaccaga taaccaaaga cataagcgcc
     6901 cacttttaac acatcaccca ctaactgcca ggcaaagaga tcgcgcatag cggtaaattt
     6961 attcgacaac agcaaccaga tagcaaaatc acgcagcaac cagacggtaa aactcgctgc
     7021 cgccactgcc ggtaagacga atttcagcga tttaaccact tcccgggtga tatcgcgctt
     7081 ttccgttagc cgcgacaacg tgggcagcaa atatacgctg aacgatgccg taataaattg
     7141 caggtaggca tcggaaatac tgctcacccc ttgccagatc cccacttcat cccagctata
     7201 ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca ggcaaggtca ccgacgtaat
     7261 caacgccatg agcgtaaatt tgctcaactg ccctgccaga ccgttatccc agctgggttt
     7321 cagatagctt aacgggatga caccacgttt aatcaacatg atggcggcag gaattaccac
     7381 cagcgcggga atcagcgcca gacccagcaa cgccccttca taaccgccca aacggtaact
     7441 gacgtagtac gcgagaacgc caatcaagct gccgacaatc agcgataacg cattacctgc
     7501 ggcatcgcga aagcctttca tcagcgccag taacaggttg ccccaggcga tccccatttg
     7561 caccagcgcc actaaacgca ccagcccctg atagtcggta ttaccaaaca atccctggct
     7621 gattggcgca gctgccagca caaaaaccag cgccatcagc gtagagaaac caagtaccat
     7681 cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt actgggcaac
     7741 gtatttggtt acaccgttaa agatgccagc cccggcaagc acgccgagca cggtaatcaa
     7801 ctggcggaaa tttgccgcca gcccaagccc cgccggacca aatgacaccg ccagcaactt
     7861 accgaccagt aacccggcac caatcttgac cagtgtactg gccgccgtcc acaaggacgc
     7921 ttttgccaac gacatatcag gagaagtagt tcaacaaagt cgcaattacc gtacgctgat
     7981 tgacgggcga caggttgtag aacagcggca ggcgcagcag gcgctcgctc tctttggtgg
     8041 tgtagcgatc ttcaccgtgg aactcaccaa agtgttcccc cgcagggcaa ccgtgcagcg
     8101 gaatgtaatg aaacaccgcc atgatttccg cttctttcag aaagttaatc aacgcgctcc
     8161 ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc gttctgcacg cagccatcgg
     8221 gaatcgacgg cagctcgata cgcccggctt tcgccagagg cgctaacgta tcgtagtagt
     8281 tttgccacag cgccagacgt tgctggttga tacgatccgc tgcttccagt tgcgcccaca
     8341 ggtacgcagc ttgcagatcg gacatcaaat agctggagcc aatatcgcgc caggtatatt
     8401 tatcgacctg accacggaag aactggctgc ggttagtgcc cttttcacgg atgatctcgg
     8461 ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc gccttcacca cccgccgtgt
     8521 agtttttggt ttcatggaag ctaaagcagc caatatgacc aatggttccc agtgcacgcc
     8581 ctttgtaagt ggacatcacg ccctgagcgg catcttccac cacaaacaaa ttatgctttt
     8641 tcgccaacgc cataatggtg tccatttcgc aggccacacc cgcgtaatgg accggcacga
     8701 taacgcgcgt tttgtcggtg atcgctgctt caatcaacgt ttcgtcgatg ttcatggtgt
     8761 ccgggcgaac atccacaaag acgattttcg cgccacgcag cacaaaggca ttggcggtgg
     8821 agacaaaggt gtagctcggc atgatcactt catcgccagg ctggatatcg agcagcagcg
     8881 ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg ctgccaaaac
     8941 gttgctccag ccactgctgg cagcgacggg taaaaccgcc atcgccacac agtttgccgc
     9001 tacccattgc cgactgcata tagtcgagtt cggttcccac caccggcggt gcgttaaatg
     9061 gaatcatgtg atcacctgta taaccagtac gcggtgcttt ctacattcgc accactttgt
     9121 atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa agttgtttta
     9181 ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct cagcacctgc accgcgtcca
     9241 gccagcaggc caattcgcgc atctgtcgca ttgagttccc gtaaagagac atagccgcga
     9301 atatcgccgg acgccgcacg tagaatcaga cattgatgat caaaggtgcc gcgcacggca
     9361 ttttcaatcc actgtgcata aaagcgaccg ctggcgtcag gcgcatacca cggcgcacga
     9421 aaacggcttt gcgcaaatgc ggcgctagct aactgacgta atgcgggaat atcggtctct
     9481 tgtgccacga cagcaccgct atcactgaca ttgttcacgg gtagcgccaa atcaacttca
     9541 ccttctacca gggagaatcc cagctgttgc agggcatcca gttcccccgt atttgatgcc
     9601 gcaattttgg cctgcacccg tgaccatggc gctaaggcgt ctgacgtcag gagcggtgct
     9661 tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc ccaggttagt
     9721 ggctcaatac tggcgcggac gggcacgaag taactccagc agatattggc cgtagccagt
     9781 tttcgctaat gaactggcag cacgcttcac accctcgtca tcgagccagc cgttacgcca
     9841 ggcgatctct tccaggcagg caatcttaaa gccctggcgt ttttctactg tctgcacaaa
     9901 agtgctggcc tcaatcaggc tgtcgtgagt gccggtatcc agccaggcaa atccgcgccc
     9961 gagcagttca acggtcaggt tgcccgcctc gaggtacatc tggttgatgg aggtaatctc
    10021 cagttcaccg cgctccgacg gcttcacctg ctttgcgtac tccacgactt tactgtcgta
    10081 gaaataaagc ccggtcaccg cccagtttga cttcggctgt tttggctttt cttcaagcga
    10141 gatagcgcgg aaattgtcgt caaattccac cacgccaaag cgttccgggt ccatcacctg
    10201 atagccaaaa accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct tcggactgaa
    10261 gccctgaccg aagaagatgt tatcgcccag caccagacaa gaaggttcac cattaaggaa
    10321 ggtttcaccg atgataaagg cctgcgccag accgtccggg ctgggctgtt cggcatattc
    10381 cagctggata ccgaactcac taccatcgcc cagcaggcgc tggaaataac ctttatcttc
    10441 cggcgtagtg atgatgagaa tttcgcggat accggccagc atcagcaccg acagcggata
    10501 gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc gcgtaatcgg
    10561 atgcaatcgg gtaccggaac cgcccgccag gataataccc ttcatgtttg cctccgctga
    10621 aaattagcct ttcagaccta aacgctcacc ctgatagctg ccgtcctgca cctgcttcca
    10681 ccagctttca ttagccagat accactgaac cgttttacgc attccacttt caaaggtttc
    10741 ctgcggcagc cagccaagtt cacgggcaat tttcgaagca tcaatggcat aacgcagatc
    10801 atgccccgga cggtcagcga caaaggtgat caggtcacga tagtacgcca cgccgtgcgg
    10861 cttgttcgaa gccagttctt ccagcagctc gcaaatggtt tccacaacat cgagattctt
    10921 acgctcgttg tgaccaccaa tattataggt ttcaccgact ttcccggtgg tcgccacgca
    10981 atacagcgcg cgggcgtgat cttccacata cagccagtca cggatttgct gcccgttgcc
    11041 atataccggc agcggtttac ccgccagcgc gttgaggatc atcagcggga tcagtttttc
    11101 cggaaagtgg taaggaccgt agttattcga gcagttggtg atcagcgtcg gcagaccgta
    11161 ggtccgcagc caggcgcgca ccagatggtc gctgctggct tttgacgcgg aataagggct
    11221 gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca ggtcaccata
    11281 tacttcgtcg gtggagatat gatgaaaacg gaacgctgat tttttatctt ccgtcagcgt
    11341 attccagtaa gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt tggtttcaat
    11401 aaacgctgcc gggccgtcaa tagaacggtc aacatggctt tcggctgcca gatgcatgac
    11461 acagtctggc tgatgctcag tgaatacgcg tgccagttct gcccgatcgc agatatcaac
    11521 tttctcaaag gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca ggtttccggc
    11581 gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt tcgttgatga tataacgcac
    11641 cagcgccgag ccaataaacc cggcaccacc tgttatcaga atttttctca tcagcgccag
    11701 actcctttgg catcgacgac atactgctga tggacattgt cgccattgat aactttgaac
    11761 tgactatgat cgaccagcat caccagcacg tctgccgttg ccagcgcctc gtcaagctgc
    11821 gccagagtac aaagcccggt cagttttttc ggcaactggt ggatgttagg ctcaacaacc
    11881 agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga tttccatcgc cgggctttcg
    11941 cgcaggtcat caatattcgg tttaaacgcc agaccaaagc aggcgatttt cagttcactg
    12001 gctcgtttat cggtagcagc aaggcagtcg gcaaccgccg ctttcacctg atcgataacc
    12061 cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga taagccgcgc ctgctggggg
    12121 ttctgtgcca cgataaacca cggatcaaca gcaatgcagt gaccgcccac gccagggcca
    12181 ggctgaagaa tattaacgcg agggtgacga ttcgccaggc gaatcagttc ccagacgtta
    12241 atcccctgat cggcacaaat cagcgacaat tcattagcaa aagcgatatt cacatcgcgg
    12301 aagctgtttt cggtgagctt acacatttcc gccgtccgcg agttagtgac gacacactca
    12361 ccttcgagga aaattttgta caattcgctg gcgcgggccg aacaaaccgg cgtcatacca
    12421 ccaatcacgc gatcgttttt aatcagctcg accataacct ggcccggtaa tacacgttcc
    12481 gggcagtaag caatgttgac gtccgcctgc tcgcccacct gctgtgggaa agtcagatcc
    12541 gggcgcatct ctgctaacca ttctgccatc ttctcggttg accccaccgg cgaggtggat
    12601 tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc agccgattca
    12661 acgtaggtca tatctggctc atgatcgcct ttaaacggcg taggtacagc aatcagccag
    12721 gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac cgccttctac ggcagttttt
    12781 actacgctcg ccaaatcagg ctcgacgata tggatttcgc cacgattgat ggtatcaacc
    12841 gcgtgttggt tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa cgctgctgct
    12901 gtaggcagcc cgatgtaacc cagtccgata acagaaatgg tcgcaaaact catagtgata
    12961 tccgattatt ttttaacgct tccagaatgc gagagcatgc ctgaccatca ccatacgggt
    13021 tatgggcgcg gctcatagct tgatattcgt tttcgtcttt taaaagacgc gtcacttcct
    13081 cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc accgcttccg
    13141 gacgctcagt ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc gcttcttcct
    13201 gaatgccgcc tgagtcagtc aaaatcagcc aggcgtggtt catcagccag acaaacggta
    13261 aatactcctg gggatcgatc agaatgacat ttttcacatg ccccagaatg cgattgaccg
    13321 gctctctgac gttcgggttg agatgcaccg gatagacaat ctggatgtcc tggtgcgtgg
    13381 tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc acgaccgaaa ctctcacgcc
    13441 tatgaccggt caccagaatc atctttttat cgggggcgat aaacgggtaa tttgccgcca
    13501 gttctgaacg cagcgtgtcg ctgctcatca cctggtcacg cacccataac agtgcatcaa
    13561 tgactgtatt accggtaatg aagattcggc tatccgcaac gttttcacgc agcaagtttt
    13621 gccgggaagt ttcggttgga gagaagtgat acatcgccag atgcccggtc aatgtacggt
    13681 tagcctcttc cggccacggc gaatagagag cgcccgtgcg cagaccagcc tcaacgtgac
    13741 caacaggaat acgctgataa aacgccgcca ggctggttgc cagcgtcgtc gtcgtatcgc
    13801 cgtgaaccag cacgacgtct ggtttgaact cggcaagaat aggttttagc ccttccagaa
    13861 tccgacaggt tatctctgtc aggccctgtc ctggctgcat tatgttgaga tcgtagtcag
    13921 gtacaatgga aaagagtttc agtacctgat caagcatctc ccgatgctgc gcagtgacgc
    13981 aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg caccaacgga gccatcttga
    14041 tggcttccgg gcgcgtacca aatacagtca gtactttcac atcgattctc ttcgaataag
    14101 cggcgagcgc ctttgcgctc accgcagcag tgttgctatt tcgagcaacg gcgggttaat
    14161 gcgacaccag ccccgatcag ccccccgaca atgccccaca taatcatcag gaaggcacga
    14221 cgtgggctat cgcgttttac cggttcttcc ggcgtacgca aatagcgata ggtctgaaaa
    14281 cgcggatcca gggttggacc aacattcagg gtgtttaaca tggctcgatt ctgatcatag
    14341 tcgagatcaa aggccggacc gacggcctgt aaattttcca gtcgagcctg gagcattgga
    14401 cgcccaagca ggaacatttc tgaatcaggt aattcctcgg caggcacatc tgtcgcactg
    14461 cgcgaaatat tatgctgctc agcaattttc agcgcctgct caatactgtt catccggcgg
    14521 tcgtagatgg ctttcgccac ctcttcctga cgcttcacct gagctttcat ctggatggtg
    14581 cgtgccgccc atgcgccttt cagctcatca ttcagatggc tggctgcacg ctggctggca
    14641 aaagcaacat actgacgtaa caggttatta gcgtcaggcg cggtttcggc aataagcttc
    14701 acgctgtcat tgaccgcgcg agtaaagtct ccggggataa actggatgtt gttaatcatt
    14761 tcatccagca acgccgcatc ggctttgctg ttgcccacca tccgctgttt gtaatagtcg
    14821 gtttgcagcc agaactctct gcgggtatcc cacgaggcca gttgcataac aaactcttta
    14881 taggcttcgt ccatgaccga tggttgatcg gcagaagcca tgtttgaacg gacatccagg
    14941 ttacgcaaaa attgctgctg cgagtaatat ccccccagca tattcaccgt tggacgatcg
    15001 gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag tatacgccag cgcgattaac
    15061 gcaaacgcca gccccatgcc aataatccat agcttcccag cccacaaggt acgaaacaac
    15121 ccacgaatat ccagttcatt ttcagcgtct tcggccggtt tcccaggcat tggttgtgtc
    15181 atcacatcct catttatttg gttaaattgg ggctgccacc acgatttcta cgcagtctgc
    15241 gttttacgcg cttaataaag cgagcaactt tccaggcacg cttaatgcaa tatccataga
    15301 ggaagaatgc tagcaaaaag agcaccagca tgacccactc cgggacaaaa tgagaatatt
    15361 ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag ggtaatcagc acaaacgcct
    15421 gacgggaagt aaacccggca cgcatgatca aatggtgaat atgctgacgg tcaggagaga
    15481 atgggctcat gcctttacgc aggcgacggt acataatcgc caccatatcc attagcggaa
    15541 tggcgattat ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc tgggtcgttt
    15601 cgagcaggat ccagataacg gtaaaaccaa tcagcgtact gcccgcatca cccataaaga
    15661 ctttgtagcg gcgacccagg ataccaaggt taagcatgat gtatggcagg atggcggcga
    15721 tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa atcataccga
    15781 ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc atcaatgcca tcaaccatgt
    15841 tgaacgcatt aatggccgcc cagacggcaa atagcgtcag gaagtaacca aacggtccga
    15901 gcaccatctc ccaggagcca aagatataac ccaggctact gagataaagt ttgccgaaca
    15961 ccatcataac aatgccaaca gcggcctgta tggtggcacg gatttttacg ctgatatcaa
    16021 aacgatcatc cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga tagagagatg
    16081 catgcggaat atagtaatcg acaattccga acgtgaagca aatccctgcg taaaccgaaa
    16141 tccccccaac gagaggtatc aatccctggt gacgtttgcg gaagtttggt ttatccacta
    16201 aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg aataaaaaaa
    16261 tactgatgag atcagtactc actgtcagta aattcacaat gcatgctctc agagaaaatt
    16321 attagcagaa gtataaccac gaagaccttt attcagaagg gaaagtccgt tccgaaccca
    16381 ccagccctgt gcaaaaatca accatttgat atgcaaattg cataacatgc cagcaaattc
    16441 caggaatttt cctaacatcg ccagcgcgac attaagatta cagatataaa agcaaaacgc
    16501 cacgtaaaca cgtggcgttt ttggcataag acaaatttat gagcgtttca tcatttcgaa
    16561 gaaatcgtca ttggtcttgg tcatggccag tttattaatg aggaattcca ttgcatcgat
    16621 ttcgcccatc gggtgaatga ttttgcgcag gatccacatt ttctgcagtt cttcctgagt
    16681 cgtgagcagc tcttctttac gggtaccgga acggttgtag tcgatagccg ggaagacgcg
    16741 tttttcagcg atcttacgag agaggtgcag ttccatgttg cctgtacctt taaactcttc
    16801 gtaaataact tcgtccattt tagaaccggt atcgataagc gccgtcgcga taatggtcag
    16861 gctgccgccc tcttccacgt tacgcgccgc accaaagaag cgtttcggac gatgaagggc
    16921 attggcgtcc acaccaccgg tcaacacttt acctgacgcc ggaacaacag tgttgtaagc
    16981 gcgcgccaga cgagtgatgg agtcgagcag aatgataacg tctttcttgt gctcaaccag
    17041 gcgtttggcc ttctcgatca ccatttccgc aacctgaacg tggcgagatg cgggttcgtc
    17101 aaaggtagaa gcaacaactt caccttttac cagacgctgc atctcggtta cttcttccgg
    17161 acgttcgtcg atcagcagaa ccatcagcac acaatccggg tggttgtaag caatgctctg
    17221 agcaatgttc tgcagcagca tagttttacc ggctttcggc ggtgccacaa tcagaccacg
    17281 ctgaccacga ccgataggtg atgccagatc cagtacgcga gcagttaaat cttcagtaga
    17341 accgttacca cgttccatac gcagacgaga gtttgcgtgc agcggggtta agttctcaaa
    17401 gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa ctttcagcag
    17461 cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta ccagagatgg tatcaccagt
    17521 gcggaggttg aaacggcgga tttggctagg ggaaacgtag atgtcatcag gaccggcgag
    17581 gtaggagctg tctgcggaac ggaggaaacc aaatccatcc tgcaatatct ccagtacgcc
    17641 atcaccaaag atatcttcgc cactctttgc gtgctgcttc aggatggcaa aaataatgtc
    17701 ctgcttacgc atgcgagcca ggttttccag ccccatattt tcgccgagag tgatcagctc
    17761 agaaaccggc gtattcttta attcggtaag attcatagtg gtgtgagttc ttaaacttgg
    17821 ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga tcatccatgc ctgtttacgg
    17881 tcatcgactc atgtcttttc gctgcctggt cacaggagag tacgcagaac tgaaacgaca
    17941 agacggattg agtgacgagc cagaaatctg ttcacttcgc atttaagata aaacgggaag
    18001 cgttggaaat tacaagattc aaacttaata aggtatgttt aatacgaagt caacactaag
    18061 ttagcatgac tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa catgaaattc
    18121 ccttacgcca ggttagcgtc gaggaactct ttcaactgac ctttagacag tgcgcccact
    18181 ttggttgccg ccacttcacc gtttttgaac agcagcagag tcgggatacc acggatgcca
    18241 tatttcggcg cagtgccagg gttttgatcg atgttcagtt ttgcaacggt cagtttgccc
    18301 tgatattcgt cagcgatttc atccagaatc ggggcgatca ttttgcacgg accgcaccac
    18361 tctgcccaga aatcgacgag gatcgccccg tccgctttga gtacatccgt gtcaaaactg
    18421 tcgtcagtca ggtgaataat tttatcgctc atatataact ccacaggaat aagcctggcg
    18481 tgttggtttc gttgttggtg taacattaac caactaaagg ttgactttat ttcaccggat
    18541 acgctttcgt aaagcaatag taagctgata ttctaccaca ctatgagcaa aacacattta
    18601 acagaacaga agttttccga cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa
    18661 aaagggtttc ataactgtac gcccattcag gcactggccc ttccgctgac gctggcgggt
    18721 cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca
    18781 acgtttcatt atcttctctc tcatcctgcg attgccgatc gcaaggtgaa tcaaccgcgt
    18841 gccttaatta tggcaccgac gcgtgaactt gccgtgcaga tccacgccga cgcggaaccg
    18901 ctggcggaag ctaccggcct gaagctgggc ctggcttacg gtggtgatgg ctatgacaaa
    18961 cagctgaaag tgctggaaag cggcgttgac attctgattg gcaccacggg tcgtttaatt
    19021 gactacgcca agcagaacca cattaacctc ggtgccattc aggtagtggt actggacgaa
    19081 gccgatcgca tgtacgatct aggctttatt aaagatatcc gctggctgtt ccgccgtatg
    19141 ccgcctgcaa atcagcgcct caacatgctg ttctccgcca cgctttcgta ccgggtacgt
    19201 gaactggcgt tcgagcagat gaacaatgcc gaatatattg aagtggaacc ggaacagaaa
    19261 acgggccacc gtattaaaga agagcttttc tacccttcta acgaagaaaa aatgcgtttg
    19321 ctgcaaacgc tgatcgaaga agagtggcca gacagagcga ttattttcgc caataccaaa
    19381 caccgttgtg aagagatctg gggccacctg gcggcagatg gtcatcgtgt cggtttgttg
    19441 acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac ccgtggcgat
    19501 ctggatattc tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc ggcagtgacg
    19561 cacgtcttta actacgattt acccgatgac tgtgaagatt acgttcaccg tattggtcgt
    19621 acaggtcgcg caggcgcaag cggtcactct atcagcctgg cgtgtgaaga gtatgcattg
    19681 aatttgcctg ctattgagac ctatattggt cactcaattc cggtaagcaa atacaatccg
    19741 gacgcattga tgaccgatct gccaaaaccg ctgcgcctca cgcgcccgcg cacaggcaat
    19801 ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat aaaatgtcat
    19861 actcaaaatc attcaaactg tatcaagatg gcaagagaat gaatcccgat gagcttactg
    19921 tagtaagtga ttcgggtgaa agaatgcagc caacacagag acagattgaa ggatgaagag
    19981 tatgggttcc acctcgtcgc tgtatgcagc cattgatctc ggttcgaata gttttcatat
    20041 gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg acgcgaatta aacgcaaagt
    20101 gcgtctggct gctggcctga atagcgaaaa tgacctctct aatgaagcaa tggagcgcgg
    20161 ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat atccctccct cgcaaattcg
    20221 cgttgtcgct acggcgacat tacgacttgc cgtcaatgcg ggtgatttta ttgccaaagc
    20281 gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt gaagaggaag cgcgtctgat
    20341 ttatcagggc gttgctcaca ccactggtgg tgccgatcag cgcctggtgg tggatatagg
    20401 cggtgccagt actgaactgg taaccggcac gggtgcacaa accacctcgt tgttcagcct
    20461 gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc tggggcaaga
    20521 aaattttgat gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt
    20581 acggtatcac ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg cgttacagga
    20641 aatcatgatg gcacagggga tggatgaacg cattaccctg gaaaagttgc agcaattgaa
    20701 acagcgagcc attcattgcg gtcggctgga ggaactggag attgacgggc tgacgctgga
    20761 acgtgcatta gtgttcccga gtggtctggc gatcctgatc gccattttta ccgaactgaa
    20821 tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct acggcatgtt
    20881 gcatctggcc gtcgagcagg atattcgcag ccgtacactg cgtaatattc aacgccgctt
    20941 tatgatcgat attgatcagg cacagcgcgt agccaaagtt gcggctaact tcttcgatca
    21001 ggtggaaaac gaatggcatc ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca
    21061 gcttcatgaa atcggcctga gcgttgactt caaacaagct ccgcaacacg ctgcttatct
    21121 ggtgcgtaac ctggatcttc ccggttttac ccccgcacag aagaaactgc tggcgacgct
    21181 actgctcaac cagactaacc cggtcgatct ctcatcgctg catcagcaaa atgccgtacc
    21241 accgcgcgtc gcagaacaac tctgccgttt actccgcctg gctatcattt ttgccagccg
    21301 tcgccgtgac gatctcgtgc cagagatgac attacaggct aaccatgaac tgttgacctt
    21361 gacgcttccg caaggttggc taacccaaca tccgctgggt aaagagatta ttgctcagga
    21421 aaaccagtgg cagagctatg tccactggcc gctggaagtg cattaatgag taagtgccgg
    21481 atgcgatgct gacgcatctt ctccggcctt gattatttcc ctcgttttgc cgccatcatc
    21541 gctttcagat tcgccagatg gctttgccct ttctgcatcc gttcttcggc actgaccact
    21601 ttgcgctcct gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa gcggctcggc
    21661 tccgggcgca ccagttcgcc gtactgacgg cgttctttac acagcgtaaa ggtcaattcc
    21721 ttctgggcgc gggtaatgcc gacataggcc agccgccgct cctcatcgat attatcttca
    21781 tcgatactgc tctggtgcgg caaaaaccct tcttccatac cgaccatgta gacataagga
    21841 aactccagcc cttttgacgc gtggagagtc atcagttgca cctgatccag ctcttcttca
    21901 ctctcaccac gctccatcat gtcgcgtaaa gtaaagcgcg tcaccacctg ggtgagcgtc
    21961 atcggctcat ccagttcgct gccttccagc atctccgtca tccagctaaa cagttggttg
    22021 acgttcttca tgcgcatttc ggcagctttc gtgctgggcg atgtttcgta cagccaggat
    22081 tcataatcca tgccgtggat cagatcgcgc accgcggcaa tcggctcccg ctccgccaga
    22141 cgctggattt ctgccaacca gtgagtgaag cgggtcaatg cttcataacc acgtccgcta
    22201 agcgtctgac tcaggcccat atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc
    22261 gcccactcac ccagcttttt cagcgtcgcc gggccaatct ctcgcttcgg cgtgttaacg
    22321 atacgcagaa atgcgctgtc atcgtccggg ttagtcagca cgcgcagata agccagcaag
    22381 tccttgattt caggacgaga gaaaaacgac gtaccaccag aaattttgta cgggatgcgg
    22441 ttttgcatca ggaatttttc aaacacccgc gactgatagt taccgcgata aagaatagcg
    22501 taatctttgt actgcgtttt attgacgaag tgatgcgcaa tcagttcacc agtgacgcgc
    22561 tcggcttcat gttcttcgtt attcgcgctt aaaactttta gctctgtgcc gtaacccagt
    22621 tcggagaaca gacgcttttc aaagacgtgc gggttattgg cgatcaggat gttcgccgct
    22681 ttcaaaatac gtccggaaga gcggtagttc tgctcaagct taatcacctt cagcgccgga
    22741 aaatcctggc tcagcagcac caggttttgc ggacgtgcgc cgcgccagga gtagatcgac
    22801 tggtcatcgt cacccaccac ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt
    22861 tcatactggc tggtgttggt gtcctgatat tcgtccacca gcaggtagcg aattttgttc
    22921 tgccagcgct cgcggacttc ttcattgcgt tgcagaagta gcgtcggcaa taaaatcaga
    22981 tcatcgaagt cgagcacatt acaggctttc aggtgtgcat catacagccc ataacaatgg
    23041 gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg cctgggacgg tgttttgaga
    23101 tcgttcttcc agttagagat ggtcgaaatc aactgttgca ggagaacttt gtcatcttca
    23161 atcagccctt cggtcaactc tttgagcaac gccagctgat cggtatcgtc aaacagcgaa
    23221 aagttcgcct tcatccccag cgccgcatac tcacgcttga tgatatccag ccccagcgtg
    23281 tggaaggttg agatcatcag cccatgcgcc tctttgcgac ccagcgtctg tccgacacgc
    23341 tctttcatct cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg tcgcgcctga
    23401 taaccgcacc cgcggatcag atgggcgatt ttattggtga taacacgagt tttaccggaa
    23461 cccgcacccg ccagcaccag gcaggggccg gtaacgaatt cgacagcttg ttgttggccg
    23521 gggtttagac gcataggaaa atcgctcaat gaaagtcggg aggggaaaat aacagcgtgg
    23581 tagtatagcc agcctcatcc ttaccactca aggcacgatc atggcaaaaa cagcagcagc
    23641 actgcatatc cttgtaaaag aagagaaact ggctctggat cttctcgtgc agattaagaa
    23701 cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag gcaaacgcgg
    23761 cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata aagttgtgtt
    23821 ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt accacatcat
    23881 taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct tgaactctga
    23941 atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg cgcagtttct
    24001 tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc agctgcgcgt
    24061 tatctaccgc accttccgga atagctttac ccaaatcgcc cggttgcagt tctgccataa
    24121 acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac tcggcagtat
    24181 cagagataac cacgttcatt tcatacagac gcttacggac gatggtgttg gcaatcagcg
    24241 gcagttcgtg cagtgattca tagtaagcag attcttcgat gatgccggaa tcaaccatgg
    24301 tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg cctttatcga
    24361 agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag gcggttttgc
    24421 cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc gccatcatac
    24481 cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagc ggtgccatga
    24541 tctctttcag ctgttcagac aacgcgtaag cacgcagttt cgccgggtta gagagacggt
    24601 ccatcatcag tgtgataccg ccctgcttca gcgcttcggt gatggtttcc caaccgaact
    24661 gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg tcgaagcaca
    24721 gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc aggtcagatt
    24781 tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg gttgctgccg
    24841 cccatgcttt agcgatcgcc atgccttcgc ctttcggatc gttttccggg tgaacggcaa
    24901 tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg ccagggcatt
    24961 tcggcgcaac catgactacg gtgatgtctt tacggatctg ctcgcccact tcgacgatgt
    25021 tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt acggtgcgca
    25081 ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc tgtgggatca
    25141 gttcttcgta agtacccact ttaaaaccat tttcggtcgc tttacgccag gacgcgcgct
    25201 tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa tcacgcatgt
    25261 tcagaccctg gttcagacct tgtgcgccac agccgacgat gactactttt ttaccctgaa
    25321 ggtagctcgc gccatcggcg aattcatcgc gccccataaa gcgacatttg cccagctgtg
    25381 ccagctgctg acgcagattc agtgtattga agtagttagc catggtgatt cctcgtgatg
    25441 ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat gacaggaaat
    25501 ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac gtcaacattg
    25561 agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc ggaaagccgc
    25621 cattttggcc gcagcgcgcg ggcgatgcac gttagcccgt ccacgctctc acggcagatt
    25681 cagcgcctgg aagaagatct cggccagccg ctgtttgtgc gcgataaccg cacggtgacg
    25741 ctgactgaag cgggcgaaga actgcgcgtt ttcgcccagc aaacgctgtt gcagtatcag
    25801 cagctgcgcc acaccatcga ccagcaaggg ccgttgctct atggcgaatt acatatcttc
    25861 tgctcggtga ccgccgctta cagccatctg ccgccgattc tggatcgctt ccgcgcggaa
    25921 cacccgtcgg tggagatcaa actcactacc ggtgatgcgg cagatgcaat ggaaaaagtc
    25981 gtcacaggtg aagctgatct ggcgattgcc gggaagccag agactctgcc cggcgcggtg
    26041 gcgttttcga tgttggagaa tctggcagtg gtgttgattg cacccgcatt gccctgcccg
    26101 gtgcgtaatc aggtttctgt cgaaaagccg gactggtcaa cggtaccgtt tatcatggcc
    26161 aatcaggggc cggtgcgccg ccgcattgag ttgtggttcc gacgcaataa aatcagtaac
    26221 ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt ggcgctcggc
    26281 tgtggcgtgg cattgttgcc ggaagtggtg ctggaaaaca gccccgaacc ggtgcgtaac
    26341 cgcgtgatga ttttagagcg cagcgatgag aaaacgccgt ttgagctggg cgtgtgtgcg
    26401 cagaaaaagc ggctacatga gccgctaatt gaggcattct ggaagatttt gccgaaccat
    26461 taacccgcca aaaagaacct gaacgccggg ttattggttt cgtcgtggca atcgtagccc
    26521 agctcattca gccgggtttc gaaatccggt tcatggtcgc caagttcgaa cgccgccagt
    26581 acgcgcccgt agtcggtgcc atggctgcga tagtggaaca aagaaatgtt ccagtgcgta
    26641 cccagcgtgt tgaggaagcg cagcagcgcg cccggtgatt ccgggaattc gaagctgtag
    26701 aggcgttcct gcaacggatg tgacggacgc ccgccgacca tatagcgcac atgcaacttc
    26761 gccatttcgt cgtcggagag atccaccacg ctgtagccgc cgtcgttgag catctgcaaa
    26821 atttctttgc gctcttcgag gccgcggctc aggcgcacgc cgacaaagat gcaggcgttt
    26881 ttggcatcgg caaaacggta gttgaactcg gtgaccgatc gcccgccaag cagttggcag
    26941 aatttgagga agctgccttt ttcttccgga atggtcaccg ccaacaacgc ttcacgctgt
    27001 tcgcccagtt cgcagcgttc cgagacgtag cgcaggccgt ggaagttcac gttggcaccg
    27061 gaaagaatat gcgccagccg ttcgccgcga atgttgtgct gggcgatata ttttttcatt
    27121 cccgccagcg ccagcgcgcc agagggttcc gccaccgcac gcacatcttc gaataaatcc
    27181 ttcatcgccg cacagatcgc atcgctatcg acggtgatga tgtcgtcgag atactcctgg
    27241 cataaacgga aggtttcgtc accgatgcgt tttaccgcga caccttcggc gaacagtccc
    27301 acgcgtggca gatcaaccgg atgacccgca tccagcgctg ctttcaggca ggcggagtct
    27361 tccgcttcta cggcgatcac tttgatttgc ggcatcagtt gtttgatcag caccgctacg
    27421 cccgccgcca gaccgccgcc gccgactggc acaaatacgc ggtcgagatg ggcgtcctgc
    27481 tggagcagtt ccagcgccag cgtgccttgc ccggcaatca ccatcggatg gtcgaacggc
    27541 ggcacccagg tgaacccctg ctgctgtgac agttcgatcg ctttagcttt cgcttcgtca
    27601 aagttcgcgc catgaagcag cacttcgccg ccgaatccac gtaccgcatc gactttgatg
    27661 tcggcggtgg cggttggcat aacgatcagg gccttcacgc ctaaccgcgc agaagaaaac
    27721 gcgacgccct gcgcgtggtt acccgcagaa gcagtgatca cgccgtgcgc tttttgttct
    27781 tccgtcaggc ccgccatcat ggcgtatgcg ccgcgcagct taaagctgtg cactggctgg
    27841 cgatcttcgc gcttcaccag aatgacgtta tcaagacgcg acgacagttt ttccattttt
    27901 tgtagcggcg taacctgcgc cgcctcgtaa accggcgcgc gcagcactgc tcttaaatat
    27961 tcggcacctt ccggagcacc ggacaggggt tgcgagtcag ccattattaa ccccccagtt
    28021 tcgatttatc gcgcaccgca cctttgtcgg cgctggttgc caggctggcg taggcgcaca
    28081 gggcaaagga aacctgacgt tcacggtttt tcggcgtcca ggctttgtca ccccgagctt
    28141 cctgcgcttc acgacgcgcc gccagttcgg catcgcttac ctgtaactga atgccacggt
    28201 tcgggatgtc gatagcgatc aggtcaccat cttcaatcag accaatgctg ccgccgcttg
    28261 ccgcttccgg tgagacgtgg ccgatggaaa gaccagaggt gccgccagag aaacgaccgt
    28321 cggtgatcag cgcacaggct ttaccgagac ccattgattt caggaagctg gttgggtaga
    28381 gcatttcctg catgccaggg ccgcctttcg ggccttcata gcggatcacc accacatcgc
    28441 ccgcgacaac tttgccaccg aggatcgctt ctaccgcgtc gtcctggctt tcgtacactt
    28501 tcgccgggcc ggtgaatttg aggatgctgt catcgacgcc cgcggttttc acgatgcagc
    28561 cgttttcagc aaaattaccg tagagcaccg ccaggccgcc gtctttgctg taggcgtgtt
    28621 ccagcgagcg gatacagccg tttgcgcgat cgtcatcgag agaatcccaa cggcaatcct
    28681 gcgagaatgc ctgtgtagta cgaatgcccg cagggcctgc gcggaacata ttttttaccg
    28741 cgtcatcctg ggtcagcata acgtcgtatt gttccagagt ttgcggcaac gtcaggccaa
    28801 gtacgttttt cacatcacgg ttcagtaacc ccgcgcgatc cagttcaccg agaataccga
    28861 taacaccacc agcacggtga acatcttcca tatggtattt ttgggtgctc ggcgcaactt
    28921 tgcataactg cgggacttta cgcgaaagtt tgtcgatatc actcatggtg aagtcgattt
    28981 ccgcttcctg cgccgccgcc agcaggtgaa gtaccgtgtt ggtagagccg cccatcgcga
    29041 tatccagcgt catggcattt tcaaacgccg ctttgctagc aatattgcgc ggcagtgcac
    29101 tttcgtcgtt ttgctcgtag taacgtttgg tcaattcaac aatgcgttta ccggcattga
    29161 ggaacaactg cttacggtcc gcgtgggttg ccagcagcga gccgttgccc ggctgcgaca
    29221 ggcccagcgc ttcggtcagg cagttcattg agttagcggt aaacatcccg gagcaggagc
    29281 cgcaggtcgg acacgcggaa cgttcaacct gatcgctctg ggagtcagaa actttcgggt
    29341 ctgcgccctg gatcatcgca tcaaccagat cgagtttgat tatctgatcg gacagtttgg
    29401 ttttcccggc ctccatcggg ccgccggaaa caaagatcac cggaatattc aggcgcaggg
    29461 aagccatcag catccccggg gtgattttgt cgcagttaga gatgcagacc atggcgtcgg
    29521 cgcagtgggc gttgaccata tactcaacgg aatcagcgat cagttcgcga gatggaagtg
    29581 aataaagcat ccccccgtgg cccatggcaa tcccatcatc caccgcaatg gtgttgaact
    29641 ctttggcaac gccgccagcc gcttcaattt gttcggcgac cagtttaccg agatcgcgca
    29701 gatggacgtg acccggtaca aattgggtga acgagttcac aaccgcgata atcggcttgc
    29761 caaaatcggc gtcggtcatt ccggtggcgc gccacagcgc acgagcaccc gccatattac
    29821 gaccatgagt ggtggtggcg gaacggtact taggcatact ttatttactc ccagtgtctg
    29881 tctcgtaaat gggacggtgc gtgccgtccc attttttgta tttattgatt aacttgatct
    29941 aaccagcccc atttatcttc ggtttcgcca gtgaagaggc cgaagaaggc ttgctgaatg
    30001 cgtttggtaa ccgggccaca acggccttcg ccaatctgaa taccgtctac gctgcgcact
    30061 ggcgtgattt ctgccgccgt accggacata aagacttcat ccgccaagta cagggattcg
    30121 cgcgacagca cctgctcacg cacttcaatt cccagctctt tcgccagttt gatgatggca
    30181 tcacgggtaa tacccggcag cgcggaggag gtgaacggtg gggtgaacag cacaccatct
    30241 ttcacttcaa acaggttttc gcctgcgcct tcagagatat aaccgttcac atccagcgcg
    30301 ataccttcct gataaccgtg gcggcgcgct tcgctaccca ccagcaggga agagagatag
    30361 ttaccaccgg cttttgccgc agttgggatg gtgtttggtg ctgcgcggtt ccaggaggaa
    30421 accatcgcat cgatcccctg ctccagcgct tctgcgccca gatacgctcc ccacgggaaa
    30481 gcggcgataa tcacatcggt tgagtatccc gctggcgggt ttactcccat gccaacatca
    30541 ccgacgaaga tcagcggacg gatataggcg ctggtgagat tgtttttgcg gatcacgtca
    30601 cgacaagctt ccatcagctc atcaatgctc tgtgaaaccg ggaagcgata gattttggcg
    30661 gagtcatgca gacgctgcat atgctcacga tggcggaata caaccggtcc tttgtgtgag
    30721 tcgtagcaac ggatgccttc aaaaaccgag gtgccatagt gcagcgcgtg cgacatcaca
    30781 tgcaccttcg cgtcttccca gcgaaccatc tccccattga accaaatgta atcagctttc
    30841 ttcgtggtca tttttatatt ccttttgcgc tcaggcgcgg atttgttgtg atgtggttgt
    30901 gctctggcag atggcaacgt gtgcgacgtc caccagttta tttaactgac taaacagtaa
    30961 gtcgaccgac cgtgggctgg caacggtcaa ttcgatattt atattttgtg catcgctggc
    31021 ggcagccata ttcattgagc agacgtggaa accacgatga cgcaccacgc gtaaaacacg
    31081 ttctaaggtt tccggattga agcgagccga tacattgacc tgatgttgca tcatgataat
    31141 ttctccaaca tttctgaatt actggcacct ggcggcacca gcggccagac gttctcaagt
    31201 tcgtcgattg agacatgaag caggtatggc ccatcactgt tcagcatggt gtcgagtgcc
    31261 gcttcaacct ggtctttacg ggtgatgtgt tggccaggga tgccgaaggc gctggctaac
    31321 atgaggaaat cggggttatc ggtaagggtg gtttcgctgt atcgttcctg aaaaaacagt
    31381 tgctgccatt gtcgaaccat ccctaaccgt tggttatcga gtaagacgat tttcaacggt
    31441 aactgcttgc gttttacggt gcccagctct tgcacattca tcatgaaaga gccgtcaccg
    31501 gagatacaga caacggtatc gttcggtcgc gcgacttgtg cgccaactgc cgccggtaaa
    31561 ccaaaaccca tggtacctaa gccgctggag gtgatgaaat tttccgggcg agtgtgggcg
    31621 atgtgctgcg cagcccacat ctggtgctgc cccacatctg tggtcacgac gcaatccgca
    31681 ggtttacgat ccgacagttg ttttaacaac aacggagcgt agatagcgtc accgggatgg
    31741 tcgtaacgcc aggaatgttc atcacgcagc tgcgcgcagt gttgctgcca gtcattgata
    31801 tttaacggct gctgtaatgc tggtaacaga gcatttaaat caccttgtaa tgccacatgt
    31861 gcctgacgca gcttgttcat ttctgccggg tcgatatcca tatggataac actggcgtgt
    31921 ggtgcgaagg tgttcagttt gccggtcacc cggtcatcaa aacgtgcacc cacggcgatc
    31981 agcaggtcac actcctgcac cgcgaagttt gccgctttgg ttccgtgcat ccccagcatg
    32041 cccagatagt acggataatc tgcttctact gcgcccagcc ctttcagcgt acaggtggca
    32101 ggcatttttg tggcagcgag aaattcacgc aaagccggaa ctgcctgcgc catccccacg
    32161 ccaccgccaa cgtacagcat cggtttttgc gcttttgcca gcatctggcg cgcttgctcg
    32221 acttcggcat gtgggaaagt cacttcgttt tcaacggtgg tgaaccacgg ttccagatcg
    32281 ccgctggcta actggatatc ttttgggata tcgaccagaa ccggaccagg acgacctgag
    32341 ctggcaacgt cgaatgcttc agccatgatg cgcggcaact cttccagaga ctgcaccaga
    32401 aagctgtgct tggtacaggc taacgacaat cccaggacat ccacttcctg aaatgcgtcg
    32461 gtgccgataa acggtgcgga cacctgacca gtgatggcaa cgacagggat ggaatctaac
    32521 agtgcgtccg caagcccggt tatcaggttg gttgcgcccg gaccagacgt ggcgatacat
    32581 acgccagttt taccggtagc acgggcataa ccgatagccg ccattgccgc accctgctca
    32641 tgtcggcaca gcaagtgctc cacgccgccg tcatacaatg catcgtaaac cggcataatt
    32701 gcgccacccg gataaccgaa aacggtgttc acaccctgtg cccgcaacgc atgtaccacc
    32761 cactgtgcgc cattcatagt tagttccccg tcctgaatct tgagaaacag aattttgtgc
    32821 tgttattcat tgtctgctcc tcggttatgt ttttaaggtc aaaaaaaccc ccggaccttt
    32881 cggtgcgggg gtcttagttc gttaaggctt gatctctaag cctttcctcg tccaagtgca
    32941 gccccgcacg gtgggataat aatcaccacc acgctaatca cgaccaggct aatcactcgt
    33001 agaagggctg tcattttgtc ttttcttgca tcttgttcga aggaatgcct aaagagttac
    33061 catagatttt gcgaatagca caagatattt ttgggcctat ttttatgatg gctttaataa
    33121 tttcaataga aattgttgtt ttttaaaaag aaaatagtga gtgaaaaaat tcaatttcag
    33181 tataaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt tgcgagcatc
    33241 attccaccgc cagaacaact caacttcact tgcacaaata aacaggaatc agccactaaa
    33301 attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg actcaaggag
    33361 ggtttatgtc actgtcaatt gttcataccc gcgcagccct ggaagtaaat gcgcccccaa
    33421 tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg ggcttaacag
    33481 aaacaacggt gaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat agcggatatg
    33541 aatatccggc gaaaaaaatc accatcaacc ttgctccagc cgatctgcca aaagaagggg
    33601 gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag cttacagcca
    33661 ataagttaga tgaatatgaa ttagtcggag aactagcgct tacaggcgct ctgcgtggcg
    33721 ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa attatcgtcg
    33781 cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg atagccgatc
    33841 atctgcaggc tgtctgtgcg tttctggaag gtaagaacac tctcgaacgc ccgaaaccaa
    33901 ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt caggagcaag
    33961 gaaaacgagg actggaaatt accgccgctg gtgggcacaa ccttttactg attgggccgc
    34021 cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca gatttaagca
    34081 atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa tcagtacaaa
    34141 aacaatggcg gcagcgcccg ttccgctcac ctcatcacag tgcgtcgtta actgcgatgg
    34201 tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac ggcgtgcttt
    34261 ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga gagccgattg
    34321 aatccgggca gatccatctt tcacgcacac gagcaaaaat aacctatcca gcccgtttcc
    34381 agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac cataaccgct
    34441 gcacgccaga acagacatta cgttatctca ctggagatcc cattaccacc ccccggcatt
    34501 ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg cgtaatggcc
    34561 gccagagagc gccaatttaa gcggcagaat aaactgaatg cctggctgga tagtccggaa
    34621 atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggttggaaga aacgctgatc
    34681 catctggggt tatcgattcg tgcctggcag cggttactga aagttgcacg aaccattgct
    34741 gatattgatc agtctgacat tatcacacgt cagcatttgc aggaggcagt tagctatcgt
    34801 gcgattgacc gtttgctcat ccatctgcaa aaactactga cataaaaaaa gggcatttcg
    34861 ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct gcggtttacc
    34921 gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact tggaccacac
    34981 gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga agagtttctc
    35041 ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat aaccatgacg
    35101 ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc cacgtggata
    35161 atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc tgcacgctcc
    35221 taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac agagtgtaaa
    35281 acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg atacggaatt
    35341 gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg ctgaatcgct
    35401 ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc aactgggtgt
    35461 gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg aaaaactact
    35521 gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg tggcgcatac
    35581 ctcacgacat aacgagtttt ctatcggtgc cagcgcctcg ttgtgggaat gtatgcttaa
    35641 tcagtggttg ggacgcttgt atcaaaatca ggatgcccat acaggcttac agttcgaagc
    35701 gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc ttgatcttct
    35761 tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg ggtatttcac
    35821 tttagcgctt tataccagtg ccccttctaa actaaaggga gatcttaact atctgcgact
    35881 ggagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg acgaagttcc
    35941 cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc ttaatggttg
    36001 cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg ttgtcgatag
    36061 cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata aaaatgcgtt
    36121 gattcgcgat cttttgaaaa ttaacgtgct ggatgatgtg tattaatctg aatggctggc
    36181 aaggatgccg gtagaaggat ttacttcgga gaggattatt tcagataaaa aaaatcctta
    36241 gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca acacccggtt
    36301 ttggagaccg gtgctctacc aattgaacta cgcccctaat tagggtggcg gaacggacgg
    36361 gactcgaacc cgcgaccccc tgcgtgacag gcaggtattc taaccgactg aactaccgct
    36421 ccaccgaatt cttttacaac caccggtttt acgaccggct t
//
LOCUS       DAJTUP010000031        34974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTUP010000031 DAJTUP010000000
VERSION     DAJTUP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.031"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..650)
                     /gene="deoR"
                     /locus_tag="OO468_001597"
     CDS             complement(<1..650)
                     /gene="deoR"
                     /locus_tag="OO468_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR6258750.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRP"
     gene            complement(708..1304)
                     /gene="ybjG"
                     /locus_tag="OO468_001598"
     CDS             complement(708..1304)
                     /gene="ybjG"
                     /locus_tag="OO468_001598"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6258751.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1589..2815
                     /gene="mdfA"
                     /locus_tag="OO468_001599"
     CDS             1589..2815
                     /gene="mdfA"
                     /locus_tag="OO468_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR6258752.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(2856..3140)
                     /locus_tag="OO468_001600"
     CDS             complement(2856..3140)
                     /locus_tag="OO468_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258753.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3226..4041)
                     /gene="ybjI"
                     /locus_tag="OO468_001601"
     CDS             complement(3226..4041)
                     /gene="ybjI"
                     /locus_tag="OO468_001601"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR6258754.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4041..5249)
                     /locus_tag="OO468_001602"
     CDS             complement(4041..5249)
                     /locus_tag="OO468_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6258755.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5333..5869
                     /gene="rcdA"
                     /locus_tag="OO468_001603"
     CDS             5333..5869
                     /gene="rcdA"
                     /locus_tag="OO468_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR6258756.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6044..7729)
                     /locus_tag="OO468_001604"
     CDS             complement(6044..7729)
                     /locus_tag="OO468_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR6258757.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            7999..8376
                     /locus_tag="OO468_001605"
     CDS             7999..8376
                     /locus_tag="OO468_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR6258758.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8406..8663)
                     /gene="grxA"
                     /locus_tag="OO468_001606"
     CDS             complement(8406..8663)
                     /gene="grxA"
                     /locus_tag="OO468_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR6258759.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8823..9110
                     /locus_tag="OO468_001607"
     CDS             8823..9110
                     /locus_tag="OO468_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR6258760.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9094..9816
                     /gene="nfsA"
                     /locus_tag="OO468_001608"
     CDS             9094..9816
                     /gene="nfsA"
                     /locus_tag="OO468_001608"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR6258761.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            9877..10779
                     /gene="rimK"
                     /locus_tag="OO468_001609"
     CDS             9877..10779
                     /gene="rimK"
                     /locus_tag="OO468_001609"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR6258762.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            10867..11343
                     /locus_tag="OO468_001610"
     CDS             10867..11343
                     /locus_tag="OO468_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR6258763.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11695..12807
                     /gene="potF"
                     /locus_tag="OO468_001611"
     CDS             11695..12807
                     /gene="potF"
                     /locus_tag="OO468_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR6258764.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12902..14035
                     /gene="potG"
                     /locus_tag="OO468_001612"
     CDS             12902..14035
                     /gene="potG"
                     /locus_tag="OO468_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR6258765.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14045..14998
                     /gene="potH"
                     /locus_tag="OO468_001613"
     CDS             14045..14998
                     /gene="potH"
                     /locus_tag="OO468_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR6258766.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14995..15840
                     /gene="potI"
                     /locus_tag="OO468_001614"
     CDS             14995..15840
                     /gene="potI"
                     /locus_tag="OO468_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR6258767.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            15900..16388
                     /locus_tag="OO468_001615"
     CDS             15900..16388
                     /locus_tag="OO468_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR6258768.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16429..17556
                     /gene="rlmC"
                     /locus_tag="OO468_001616"
     CDS             16429..17556
                     /gene="rlmC"
                     /locus_tag="OO468_001616"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR6258769.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17731..18462)
                     /gene="artJ"
                     /locus_tag="OO468_001617"
     CDS             complement(17731..18462)
                     /gene="artJ"
                     /locus_tag="OO468_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR6258770.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18754..19422)
                     /gene="artM"
                     /locus_tag="OO468_001618"
     CDS             complement(18754..19422)
                     /gene="artM"
                     /locus_tag="OO468_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR6258771.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19422..20138)
                     /gene="artQ"
                     /locus_tag="OO468_001619"
     CDS             complement(19422..20138)
                     /gene="artQ"
                     /locus_tag="OO468_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR6258772.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20145..20876)
                     /gene="artJ"
                     /locus_tag="OO468_001620"
     CDS             complement(20145..20876)
                     /gene="artJ"
                     /locus_tag="OO468_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6258773.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEIITVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20894..21622)
                     /gene="artP"
                     /locus_tag="OO468_001621"
     CDS             complement(20894..21622)
                     /gene="artP"
                     /locus_tag="OO468_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR6258774.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21840..22355)
                     /locus_tag="OO468_001622"
     CDS             complement(21840..22355)
                     /locus_tag="OO468_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6258775.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23004..24773
                     /locus_tag="OO468_001623"
     CDS             23004..24773
                     /locus_tag="OO468_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6258776.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            24984..25307
                     /locus_tag="OO468_001624"
     CDS             24984..25307
                     /locus_tag="OO468_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR6258777.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25304..26134
                     /gene="amiD"
                     /locus_tag="OO468_001625"
     CDS             25304..26134
                     /gene="amiD"
                     /locus_tag="OO468_001625"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR6258778.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26131..27144)
                     /locus_tag="OO468_001626"
     CDS             complement(26131..27144)
                     /locus_tag="OO468_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR6258779.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27243..28673)
                     /locus_tag="OO468_001627"
     CDS             complement(27243..28673)
                     /locus_tag="OO468_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR6258780.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28684..29685)
                     /gene="ltaE"
                     /locus_tag="OO468_001628"
     CDS             complement(28684..29685)
                     /gene="ltaE"
                     /locus_tag="OO468_001628"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR6258781.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29722..31440)
                     /gene="poxB"
                     /locus_tag="OO468_001629"
     CDS             complement(29722..31440)
                     /gene="poxB"
                     /locus_tag="OO468_001629"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR6258782.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31573..32541)
                     /gene="hcr"
                     /locus_tag="OO468_001630"
     CDS             complement(31573..32541)
                     /gene="hcr"
                     /locus_tag="OO468_001630"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR6258783.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32553..34205)
                     /gene="hcp"
                     /locus_tag="OO468_001631"
                     /gene_synonym="priS"
     CDS             complement(32553..34205)
                     /gene="hcp"
                     /locus_tag="OO468_001631"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR6258784.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34349..>34974)
                     /gene="lysO"
                     /locus_tag="OO468_001632"
     CDS             complement(34349..>34974)
                     /gene="lysO"
                     /locus_tag="OO468_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6258785.1"
                     /translation="HQQEKLPSRIAMALESLKLCGVVVIGFAIGLSGLAFLQHATEAS
                     EYTLILLLFLVGIQLRNNGMTLKQIVLNRRGMIVAVVVVVSSLIGGLINAFILDLPIN
                     TALAMASGFGWYSLSGILLTESFGPVIGSAAFFNDLARELIAIMLIPGLIRRSRSTAL
                     GLCGATSMDFTLPVLQRTGGLDMVPAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8670 a         8662 c         8993 g         8649 t
ORIGIN      
        1 ggacgcacct tgccaaattt actgtggtcg acaaccagca catgcttttg cgccatcgac
       61 atggcccagt gttttaccgg caactcttca agattaaaac aggtagcgcc tttactgaca
      121 tgcacgcccg ccgcggaata aaaagcgata tccgggcaaa aattattcaa cgtttgctga
      181 aaatcgatgg gtttgaaaat ggcgttgctg gcgtgaaatt caccaccgca cagaaatgcg
      241 cggcaatggg gtttctcttt cagcgccaga aaggtattta gcgaataaca aacggcggta
      301 aaagggattt cattatcaat cgcttcaata atccacggcg tggtggtgcc acagtcaaaa
      361 aagagggtct gatcgggttc taccagtgtc gcagccagtt ttgccgcccg gcgtttttct
      421 tccaccaggc gggatttttg atcgcttaac aggtaatggc tggcactgcg cggttccaga
      481 acaatatagc cgccgagcaa aacgacgggc gcactgtggt tgttcagatc gcgacgaatc
      541 gtcatctccg aaaccccaag cagggcggcg gcgtctttaa gatgtaactt atcgctgcgt
      601 tttaattctt gcagcagctg cccgatacgc tcttcgcgac gtgtttccat aatccctctg
      661 aatagttatt gaagcgagcc gctcaatact acacttttta gcagagatca gtcacgcacc
      721 cagcctttgc ggatcggtaa tgcaaaacag acgcgatacc acgattgcag acgttgatag
      781 agtttatgcc ccatcgcttg ccagataatc tgggcactaa ggcagccaat catacctgcc
      841 agcaatccac cgagcatatc cagcggccag tggacgccaa gataaacgcg cgaccaggca
      901 atgacgacgg ccagcaccat taaaagtgag ccggaccaca ggcgatgcca gcataaaaat
      961 gccagtgcaa aggtgaaaat caccgtaccg tgatcgctcg ggaatgagtc atccgccgca
     1021 tgatgcagga agttatagcc gatattttcg acaaagggtc ggtcgtgcgg aaaaagatgt
     1081 cccatcgtcc aggacacaaa caggctgacc gccagcgcga tagcgatttt tatcaccagt
     1141 tgccgttgtg ctgtaagccc ccacaaccaa agtaccgcgg ccagcaacgg caccacggta
     1201 atcaaatctt tagcaataaa aatcgccaac gagatcatcc acggagccga gtctggcgtc
     1261 gcgttaataa gagagaatag agagagattc aaattttcca gcataacttc ccgacgcaaa
     1321 gtgattaaaa gggagccaat acaggcaagt cgttgagaat aaagtgcagg ttaaactggg
     1381 taaagcggca tcgtcttatt tccctcaagc ggcctgttta cggtgggtga ttgtaacggg
     1441 cataggttaa ataaaactta aagaaagcgt agctatactc gcaataatgt aagaatgtgc
     1501 ttaaccgtgg tttcagctac aaaattcgct ttctcgttag ctgcgctttt attaaactct
     1561 gcgcgattat tattggcgaa gaaattgcat gcaaaataaa ttagcttccg gtgccaggct
     1621 tggacgtcag gcgttacttt tccctctctg tctggtgctt tacgaatttt caacctatat
     1681 cggcaacgat atgatccaac ccggtatgtt ggccgtggtg gaacaatatc aggcgggcat
     1741 tgattgggtt cctacttcga tgaccgcgta tctggcgggc gggatgtttt tacaatggct
     1801 cctggggccg ctgtcggatc gtattggtcg ccgtccggtg atgctggcgg gtgtggtgtg
     1861 gtttatcgtc acctgtctgg caatattact ggcgcaaaac attgaacaat tcaccctgtt
     1921 gcgcttcttg cagggcataa gcttctgttt cattggcgct gtgggatacg ccgcaattca
     1981 ggaatccttc gaagaggcgg tttgtatcaa gatcaccgcg ctgatggcga acgtggcgct
     2041 gattgctccg ctacttggtc cgctggtggg cgcggcgtgg atccatgtgc tgccttggga
     2101 ggggatgttt gttttgtttg ccgcattggc agcgatctcc tttttcggtc tgcaacgagc
     2161 tatgcctgaa accgccatgc gtataggcga gaaactgtca ctgaaagaac tcggtcgtga
     2221 ctataagctg gtgctgaaga acggccgctt tgtggcgggg gcgctgggat tcgttagcct
     2281 gccattgctg gcgtggatcg cccagtcgcc gattatcatc attaccgggg agcagttgag
     2341 cagctatgaa tatggcttgc tgcaagtgcc tattttcggg gcgttaattg cgggtaactt
     2401 gctgttagcg cgtctgacct cgcgccgcac cgtacgttcg ctgattatta tgggcggctg
     2461 gccgattatg attggtctgt tggtcgctgc tgcggcaacg gttatctcat cgcacgcgta
     2521 tttatggatg accgccgggt taagtattta tgctttcggt attggtctgg cgaatgcggg
     2581 actggtgcga ttaaccctgt ttgccagcga tatgagtaaa ggtacggttt ctgcggcgat
     2641 gggaatgctg caaatgctga tctttaccgt cggtattgaa atcagcaaac atgcctggct
     2701 gaacgggggc aacggactgt ttaatctctt caaccttgtc aacggaattt tgtggctgtc
     2761 gctgatggtt atctttttaa aagataaaca gatgggaaat tctcacgaag ggtaaaaaaa
     2821 tgcctgattg atttgtgcga tcaggcattc tcgaattaat ggtgatggtc gtcaatctgg
     2881 tgttcgataa ccatcccttc acctacgctg gcaagatggc gaacataagg atgcgggcgg
     2941 taagccggag ctggcgcagg agccacatag acggtttgcg gcacagtcgc gacgctgacc
     3001 gcttgtggaa cgctgactga atcaggaacc acaaccactt tgtatccact cggcacatcg
     3061 acggtgacac tttgcgccat cgccacacca gtaaagccca ttaaaagcgc gcccaacagt
     3121 agacaatttt tcataatcat ttcgcctgaa ataatgcagc gggttaattt ccgcgaatta
     3181 tgcagccttc attcaggcat tgtgttgccg ggcgtgctgc cgtttttatt ggtcaaatgg
     3241 cgcttcgtgt ttaagaactt tatcgatcac atccagtacg ccttcacggt tattgtagcc
     3301 tgcccggtat tttgccgctg cgacgactgc gctgccggca ttttccattg caaaactaaa
     3361 gcctgcctga cgcagcatct caatatcgtt accgccatcg ccaaagacca ccacttcgct
     3421 gtcgtctatt ccccataatt tctgcagttg gcgaaggcca ttggctttat gtacgccggg
     3481 gataatcaga tcgatgctgc cgttgccggt gtggactggc accataatat cgccgatggc
     3541 ctcatgtaat gctttttgta cttgtggaat cagttcatcg gaaagattca ggccaaactt
     3601 aaagaaaata tcttctaagt tgtcaaagtt atcgacgtat tccagacggt gataatacat
     3661 ttccgccacc gttttcatgg catcgtcata ttttttgagt gtataggcgc tattttttcc
     3721 gcaggcaata atttccactt ccgggcgcgt cagcaaatgt tccacgacag tagcaaacgc
     3781 atccttcgac agctcgccat taaaaacatc tttgccttca ctcattaccc agccgccgtt
     3841 ttcagccaca aaggcaattt cattcgcaat ttcagggaag aaagagatca actgataata
     3901 ttgattcccg ctggcgacca caaagcgaat gccttgtgct ttcatttgct gatactgagc
     3961 cataaaccgc tcacggttat aggttttttg atcgcttaag aaagtaccat ccatgtctac
     4021 cgcaattaat ttaatgctca tcaactattc tccatcgccg tctgcgtttt ggtatcgggt
     4081 ttggcgacgg ctttcgcgac aatagccgcg agaataacca gcgccagtac aaccagcatt
     4141 gcactacgta atccataatg ttcgccgaga tagcccagca gcggcggccc gacgaggaaa
     4201 gccagataac cggtcgttgc taccacactg acgcgggttg gtgcatcggg gccggtatcg
     4261 ctggcggcag aaatggtcag cgggaagccc agcgaggcac ccagtcccca aagtacaaca
     4321 gacactcctg cgacccaggc gctatcgaca aaaataatca gcccaatacc caacgccccc
     4381 attagtgcac tggcccgaac cacggcaacg cgactgtaac tgtcgatgaa ccaaccgccg
     4441 gtgaagcgtc caacggtcat ccccagggta aaaccggcat aaatcagtga gccggaagtg
     4501 gggctaaaac cgtgaccatc aaccattaat aagggtaacc agtcgttggc agaaccttcg
     4561 gcaaaggcca tcgccagcac tacaacacca atcaacagca actggatatc gcgataaaaa
     4621 ggaatgcctt tttcgccatg ctgggtgcca tcggcagcat ttttgcccgt accgtcaggg
     4681 attgcctgaa tggcgatata aataggcgcg atacctacca gcgccgccaa taaaatgtgc
     4741 accgttgccg gaacgccaaa ggccgtcagt gccatcccga caccagcgcc tgccagcgtg
     4801 cccaggctat aaaaaccgtg catcatcggc aaaaccgttt tattcatttc tcgctcaacg
     4861 gcggcacctt caacgtttat cgccacttcc gcagaaccaa aacttgcccc aaagacgccg
     4921 agaccaacgg caaagagcag gggcgatgtc agccagagtg ccagacttaa tatcatcatc
     4981 ccgatcaatg cgcaggacat cgtgaccagg atgacattac gtgtcccaaa gcgtttcact
     5041 aaccacgccg agcagagaat accgctcatc gaaccgatcg acagaccaaa gagaacaccg
     5101 cccatttcag cgatcgagac agagagaata tcgcggatag caggcgtacg ggttgcccag
     5161 gacgccatta acaggcctgg caaaaagaag aacataaaca gcgcccaggt tcggcgtttc
     5221 aatgcattac gtgaagaatt tacggtcata gatcacgtca aaataagaag agggaagaca
     5281 acattagcaa ggttgtgtac atttgtacac aattgcagac agaggaaatg acatgcgtcg
     5341 cgctaacgat ccgcaacggc gagaaaaaat tatccaggcc acgctggagg cggtgaaact
     5401 ttacggaata catgctgtta cgcaccgcaa aattgctacc cttgccgggg taccgttggg
     5461 atcgatgacc tactattttt caggaattga tgagttgttg ctggaggcgt tcagcagttt
     5521 tactgagatt atgtcccggc aatatcaggc attttttagc gatgttagtg atgctcaagg
     5581 cgcatgccag gctatcaccg atatgatcta cagctcacag gttgcaacgc cggataatat
     5641 ggagctgatg taccagctct acgcgctggc tagccgaaaa ccgctattaa aaacggtaat
     5701 gcaaaactgg atgcagcgca gtcagcaaac gctcgaacaa tggtttgaac ccggaaccgc
     5761 ccgcgcgctt gatgcgttta ttgaggggat gacgctgcat tttgtcaccg accgtaagcc
     5821 gctatcgcgc gaggagattt tgaggatggt tgagagggtt gcagggtagt agataaattt
     5881 tagataacaa aaaacccatc aaccttgaac cgaaatggcg gggttgatgg gctccacaaa
     5941 atggggacat caaagaaaag cagtggcact aattaagact gatgccctgc ggaaaagttc
     6001 tgcggttgtg caaaaaaatt tcattttcag ggcaacttca gttttatcct aatcctggcc
     6061 ataccatgac gatgattgtc cctgccagcg tcagcaggac gttggcgatt gcgtaggtgc
     6121 ccgcatagcc cagcgcaggg atgttactgc gagctgtatc actgatgatc tccattgccg
     6181 gcgcgcaggt gcgtgcgccc atcattgcgc cgaacaacag tgcgcggttc attcgcaata
     6241 cataagcacc gaacaagaaa cagataacca cgggcacaag actgacaatt aatccggcaa
     6301 tcaacatctg accgccaatc gcgcccaggc cgttattaat accgctaccg gcgctcagac
     6361 caacgcctgc cataaacacc atcaagccga actctttcac catgcttaat gcaccttgcg
     6421 gaatgtaacc gaaggtcggg tggttagcac gcataaagcc cagcataatt ccggcgaata
     6481 acaacccggc agcgttcccc atgccgaaac tgaatgtgct gaactggaag gtgatcatcc
     6541 cgatcatcag cccaataaca aagaaggcgc aaaatgccag caggtcagtg acctggctgt
     6601 gaatcgagat aaagccgatg cgatcggcga tggtttttac gcgacgggca tcaccgctga
     6661 cttgtaaaac gtcacctttg ttaagcacga cgttgtcatc tatcggcatc tcaatctggc
     6721 tacgaatgac gcggttaagg aagcaaccgt gatcggtcaa cttcagttgt gcgagacgtt
     6781 tacctacagc gttatggttt ttaacgacca cttcttcagt gacgatacgc atgtcgagaa
     6841 ggtcacgatc gaaaacttct ttaccgttac ggaagctggg atcgagtcgg gcatgggcgt
     6901 cgggatagcc taccaacgct atttcatcgc ccatttgtag cacggcatca ccgtctggat
     6961 ttgccagaat cccgttacgt cgaatacgtt caatgtagca gccggtttgt cgataaatac
     7021 ccagttcacg cagatttttg ccgtcggtcc aggccaccag ctccgggccg acgcgatagg
     7081 cgcggatcac cggtaaataa accttacggt tggcatcagt gtccaggcca cgttcgcggg
     7141 cgatttgctg ggcgctggtc tgtaagtcct gatgctgcaa tttcggcaag taacgcgcac
     7201 caacaatcaa actcaccaga ccgattaaat aggttaaggc atacccgagg ctcagattat
     7261 ccagtgccag tgagagctgc ctgctttcca tgccggaatg atgcagtgta tcgccagcac
     7321 cgaccagaac cggtgtcgac gtcatagagc ctgctaacat accggccgtc aggccaatat
     7381 cccagccaaa cagcttacct aaccctaagg cgatcaccag cgcactgcca accatcacca
     7441 gtgctaacat taggtaattt ttcccatcgc gaaaaaaaat ggaaaaaaag ttcggtccgg
     7501 cttcgacccc gacgcagaaa ataaacagca taaagccaag attaagcgca tcggtgttaa
     7561 tgctgaaatg ttgttggcct aataacagcg atacgactaa aacgccaatg gaattaccca
     7621 gttggatcga accaagtcgt aactttccga gacatagccc aagcgcgagg accacaaata
     7681 ataacagaat gtaattccca tttaacaatt cggcgacgtt tatattcacg gaggctaact
     7741 tcttgtttac tagtaagctg ttgaaagaaa tgctaattta cgataatgtg ttttaccaga
     7801 attcagggcg cagattcatt cagcgcacct aaacgatagt aaagtaacaa tatattttac
     7861 tagtgtaatc acattaggta tcaacggcta tatgaattgc gttggcctat attagcatgg
     7921 aatgcgaagc ggctttatct tactgaacgc cacactggcg aaaaatgtgt tcgatagacg
     7981 cagtgtcagg aggaacgagt gaaacataaa caacgttggg cgggggcaat ctgctgtttt
     8041 gtcctcttca ttgtggtgtg cctttttctg gcgacgcaca tgaaaggcgc ttttcgggct
     8101 gccgggcatc ctgaaatcgg cttgctgttt ttcattcttc ctggagcagt tgccagtttc
     8161 ttttcacagc gtagagaagt cctgaaacct ctatttggcg caatgctggc ggcaccctgt
     8221 tcaatgctca ttatgcggct gtttttttca ccgacgcgct cattctggca agagctggca
     8281 tggttactaa gcgcggtgtt ctggtgtgcg ctgggggcac tgtgtttctt atttatcagt
     8341 agtttgttta aaccacagca cagaaaaaat cagtaaagcc ctcaacgcga gggcttgtca
     8401 gacgatcagg cgtccagatt ttctttcacc catgcagcaa aatcggtata gccgccgata
     8461 tgttgctgat cgacaaaaat ctgcggcacg gtttctacgg gtttacctgc cttttgttgt
     8521 agatcttctt tagtgatccc ttccgcacga atatctacat actgatactg aaaatcatcg
     8581 cgttcattgc tcaatttctc agccagatct tttgcacgca cacagtaagg gcaacccgaa
     8641 cgaccaaaaa taacggtttg cattatttct ctcctcatag atttatgcct gtaatgatca
     8701 cgctaaaatg tattcgctga aagtaggttt aacctgttgc attaattgct aaaagctata
     8761 actgttaaac acaatacagt gaaaagtttt agactgaagg ctcactttgc agagggaagc
     8821 gtatgcgcgc gatcggtaaa ttgcctaaag gcgtgttgat actggaattt atcggaatga
     8881 tgctactggc ggtggcgctg ctgtcggtaa gcgactccct gtcgctgcct gagccatttt
     8941 ctcggccaga agtgcagatt ctgatgattt ttctcggtgt tttgctcatg cttcccgctg
     9001 cggtggtggt tattcttcag gtggcaaaac gtcttgcccc acagctgatg aactgtccac
     9061 cgcaatattc acgttcagaa agagaaaaag ataatgacgc caaccattga acttatttgt
     9121 ggccatcgcg ccattcgcca tttcactgat gaacccattt ccgaagcgca gtgtgaggcg
     9181 attattaaca gcgcccgtgc gacgtccagt tccagttttt tgcagtgtag tagcattatt
     9241 cgcattaccg acaaagcatt acgtgaagaa ctggtgacgc tgaccggcgg gcaaaaacac
     9301 gtagcgcaag cggcggagtt ctgggtgttc tgtgccgact ttaaccgcca tttacagatc
     9361 tgtccggatg ctcagctcgg cctggcggaa caactgttgc tcggtgtcgt tgatacggca
     9421 atgatggcgc agaatgcatt aaccgcagcg gaatcgctgg gattgggcgg ggtatatatc
     9481 ggcggcctgc gcaataatat tgaagcggtg acgaaactgc tgaaattacc gcagcatgtt
     9541 ctgccgctgt ttgggctgtg ccttggctgg cctgcggata atccggatct caagccgcgt
     9601 ttaccggcct ccattttggt gcatgaaaac agctatcaac cgctggataa agacgcactg
     9661 gcgcagtatg acgagcaact ggcggaatat tacctcaccc gtggcagcaa taatcgctgg
     9721 gatacctgga gcgatcatat ccgccgaaca atcattaaag aaagccgccc atttattctg
     9781 gattatttgc acaaacaggg ttgggcgacg cgctaaaacc gccacgtcga tgtatgatac
     9841 gcgggctttt gaccaggtct gacagagagg tgcagggtga aaattgccat attgtcccgg
     9901 gatggaacgc tctattcgtg taagcggctg cgtgaagccg ctatacagcg cggtcacctg
     9961 gttgaaattc ttgatccgct ttcttgctac atgaacataa atcctgcggc gtcttctatt
    10021 tactacaaag gccgcaagtt accccatttt gacgcagtga tcccgcgtat tggcaccgcc
    10081 attacctttt atgggacggc ggcactgcgc cagttcgaga tgctggggag ctatccgctc
    10141 aatgagtcgg tcgccattgc ccgggcgcgt gacaaattgc gttccatgca actgctggcg
    10201 cgtcagggca tcgacctgcc tgtcacgggc attgcgcatt cgccggatga taccagcgat
    10261 ttaatcgaca tggtcggtgg tgcgccgctg gtggtcaagt tggttgaagg cacgcaggga
    10321 attggcgtcg tgctggcgga gactcgtcag gcggcggaaa gcgtgattga cgctttccgc
    10381 ggtctgaacg cgcatattct ggtgcaggaa tatatcaaag aggcgcaagg gtgcgatatc
    10441 cgctgtctgg ttgttggtga tgaagtggtc gctgcgattg aacggcgggc gaaagagggc
    10501 tattttcgtt ccaatttgca tcgtggcggc gcggcaagcg tcgccagtat cacaccacag
    10561 gagcgtgaaa tcgcgataaa agccgcgcga acgatggcgc tggacgttgc tggtgtggat
    10621 attctgcgtg ctaatcgcgg gccgttggtg atggaagtga atgcgtcgcc ggggctggaa
    10681 ggaatagaaa aaaccaccgg tatcgatatc gcgggtaaaa tgatccgctg gatcgaacgc
    10741 cacgctacga cagaatattg cctgaaaacg ggtggttagt cgcaatcaca ttactgatca
    10801 tggttttgcc tgcgcttttt gcgtaagctg tgccggtctt tttatcgaaa gaggttgtac
    10861 aaaattatga catcgctggt cgttcctggt ctggatacgc tgcgtcaatg gctcgatgac
    10921 ctggggatga gtttttttga atgtgataac tgtcaggctc tgcatctgcc ccatatgcag
    10981 aatttcgacg gtgtctttga tgccaaaatc gatctgatcg ataacacgat cctgttttct
    11041 gccatggcgg aagtccgacc ttcagccgta ttgccgctgg cggcggattt atctgccatc
    11101 aatgccagtt cgctgaccgt gaaagcattt cttgatatgc aggatgataa tctgccaaag
    11161 ctggtggttt gccagtcttt atccgttatg cagggcgtaa cctatgagca gtttgcatgg
    11221 ttcgtgcgtc agagcgaaga gcagatttcg atggtcattc ttgaagctaa tgcccatcaa
    11281 ctgctgttac cgactgacga tgaagggcaa aacaacgtta ccgaaaacta ttttctccac
    11341 tgataattcc tttcgagcac gcagtcgctg ccgcagtgac tgcgcgctgc aaaattatct
    11401 gctgttttta accttttctt aaagattatt tcacttctct tgtgtcgatt tggctttatc
    11461 acatagagca aatacgcata aaaatttgtt aaataccgtt ttttaatccg agctatagtc
    11521 tcaaaccctg gctaaagtta ttcttgcgat gcttctatat agcgagcagt gctggccggg
    11581 agagagttct cttttcttac accgcgccga taaaaaaata tgcacgttta ttgcatatct
    11641 ttcagtgtga caacttttgt tcgtttgtta acgaactttc agaaggaaag agatgtgacc
    11701 gccttaaata aaaaatggct atcgggtctg gttgcgggtg ctctgatggc cgtctctgtc
    11761 ggcacgctcg cggctgaaca aaaaacactc cacatttata actggtctga ttatatcgcc
    11821 cctgacacgg tggccaattt tgaaaaagaa accggtatta aagtcgtcta cgatgttttc
    11881 gactctaacg aagtactgga aggcaaatta atggcgggta gcaccggctt tgacttggtg
    11941 gttccatctg ccagctttct agaacgccag ttgactgcgg gagttttcca gccgctggac
    12001 aaaagcaaat tgccggagtg gaagaatctc gacccggagc tgctgaagct ggtcgccaaa
    12061 cacgatcccg acaataaatt tgctatgccc tatatgtggg cgacgaccgg gattggctat
    12121 aacgttgata aagttaaagc tgtgctgggc gaaaacgcgc ccgtcgatag ctgggacttg
    12181 atcctcaaac ctgaaaatct ggaaaaactg aaaagctgcg gtgtctcttt cctggatgcg
    12241 ccagaagaag tttttgctac cgtgttgaat tatctcggca aagatcccaa cagcactaaa
    12301 gcggatgatt acaccggacc ggcaaccgat ctgctgttaa agctgcgccc gaacattcgt
    12361 tatttccatt catctcaata cattaacgac ctggcaaacg gcgatatttg cgtcgctatc
    12421 ggctgggcag gtgatgtctg gcaggcgtca aaccgcgcga aggaagcgaa gaatggagtg
    12481 aatgtctcgt tctcgattcc aaaagaaggg gcgatggcgt tctttgatgt attcgccatg
    12541 ccagcggatg ccaaaaacaa agacgaagcc tatcagttcc tgaattacct gctgcgcccg
    12601 gatgtagtag cgcatatttc cgaccatgtg ttctatgtca acgccaataa agcagccacg
    12661 ccgctggtga gtgcggaagt ccgtgagaac ccgggtattt atccgcctgc ggatgttcgt
    12721 gcgaagctgt tcactctgaa agtgcaggac ccgaaaatcg accgtgtgcg cacccgcgcg
    12781 tggaccaaag tgaagagcgg aaaataatcc gcagccttag atgccgggcg ggcgcaccac
    12841 acccgccggc aattcgcacc atcatggtgc gcttgcacac attcaatgcc ggagagcagc
    12901 cgtgaatgac gctatccctc gcccgcaggc gaaaacccgt aaggcgctga cgccgctatt
    12961 agaaatccgc aacctcacca aatcctacga tggtcaacat gcggtggatg atgtcagcct
    13021 gaccatctac aaaggtgaaa tcttcgcgct gctgggcgca tccggctgtg gcaagtccac
    13081 gctgctgcgt atgctggcag gtttcgaaca accttctgcc ggacagataa tgcttgatgg
    13141 cgtcgatttg tcacaggttc cgccttacct gcgccccatc aatatgatgt ttcagtctta
    13201 cgcgctgttt ccgcatatga ccgtggaaca gaacatcgct tttggcctga aacaggacaa
    13261 actaccgaaa gcggaaattt ccagtcgggt caatgagatg ctcggcctgg tgcacatgca
    13321 ggagttcgcc aaacgtaaac cgcatcagct ttctggtggt cagcgacagc gtgtggccct
    13381 ggcccgaagc cttgccaagc gcccgaaact attactgctc gacgagccga tgggcgcgct
    13441 ggataaaaag ctgcgtgacc ggatgcagct tgaagtggtg gatattctgg agcgcgtcgg
    13501 tgtgacttgt gtgatggtca cccacgatca ggaagaggcg atgaccatgg cggggcgcat
    13561 cgccattatg aatcgtggga aatttgtcca gattggcgaa ccggaagaga tctacgagca
    13621 tccgactacc cgctatagcg ccgaatttat tggctcggta aacgtctttg aaggcgtact
    13681 caaagagcgt caggaagatg gcttggtgct tgattcgccg gggctggtgc atccactgaa
    13741 agtcgacgcg gatgcctcgg tggtcgataa cgtgccggta catgtggcgc tgcgcccgga
    13801 aaaaatcatg ctttgcgaag agccgcccgc caatggttgt aacttcgcgg tgggggaggt
    13861 gatacacatt gcctatctcg gcgatctttc ggtgtatcac gttcgtctga aaagtgggca
    13921 gatgattagt gcccagctac aaaacgccca tcgtcatcgt aaagggttac cgacctgggg
    13981 cgacgaagtg cgtttgtgct gggaagtgga cagctgtgtg gtgctgacgg tttaaggagc
    14041 aaagatgaat acacttgaac ctgctgccca gtcgaaaccg ccgggcggat ttaagctgtg
    14101 gttgtcgcag ctgcaaatga agcatgggcg caaactggtc attgcgttgc catatatctg
    14161 gttgatcttg ctgtttctgc tgccatttct gattgtcttt aaaataagcc tggcggagat
    14221 ggcgcgcgct attccacctt ataccgaact gatggagtgg gctgacgggc aactgtccat
    14281 cactcttaat ctcggtaatt ttctgcaact gaccgacgat ccgctctatt tcgatgctta
    14341 tctccagtcg ctacaggtgg ctgcgatttc gacattttgc tgtttactga tcggctatcc
    14401 gctggcgtgg gcggtggcgc acagtaagcc ttcgacccgt aatattttat tactactggt
    14461 gatcctgccg tcgtggacct cgtttctgat ccgcgtttat gcttggatgg gaatattaaa
    14521 aaacaacggt gtgctgaata attttctgct gtggctgggg gttatcgatc aaccgctgac
    14581 cattcttcat accaatctgg cagtttatat tggcattgtt tacgcctacg tgccgtttat
    14641 ggtgctgccg atttataccg cgttgattcg tattgattat tcgctggtgg aagcagcgct
    14701 ggatctcggt gcacgaccgc tgaaaacgtt ctttactgtg atcgtgccgc tgactaaagg
    14761 tgggattatt gccggatcga tgctggtgtt tatcccggct gtgggcgagt ttgtgatccc
    14821 ggaactgctc ggtggcccgg acagcatcat gatcgggcgc gtgctatggc aagagttttt
    14881 caataaccgc gactggccgg tggcctcggc ggtagcgatc atcatgttgc tgctgctaat
    14941 tgtgccgata atgtggttcc acaaacacca gcaaaaaagc gtgggagaac acggatgaat
    15001 aatttaccgg tagttcgttc gccctggcgg attgtgattt tgctgctggg ctttaccttt
    15061 ctctacgcgc caatgctgat gctggttatc tattcgttta acagctcgaa gctggtgacg
    15121 gtgtggggcg gctggtcaac gcgctggtat ggtgagttat tgcgcgatga cgcgatgatg
    15181 agtgcggttg gttcaagcct gacaattgct gcctgtgcgg caacggcggc ggcgatcctc
    15241 gggactattg cggcggtggt gctggtgcgc tttggcaggt ttcgcggatc aaatggcttt
    15301 gcctttatga tcaccgcgcc gctagtaatg ccagatgtca tcacgggctt gtcgctgttg
    15361 ttattattcg tcgcgcttgc tcatgccatt ggctggcctg cggaccgcgg tatgcttacc
    15421 atctggctgg cgcatgtcac tttctgtacg gcttatgtgg cggtcgttat ttcgtcgcgt
    15481 ctgcgggaac tggatcgctc gatagaagaa gccgcgatgg atctcggtgc gacgccgctg
    15541 aaagtgtttt tcgtcattac gctaccgatg atcatgcccg cgatcatttc tggctggtta
    15601 ctggctttta ctttgtcgct tgatgatctg gtgatcgcca gctttgtttc cgggccggga
    15661 gccaccacgt taccgatgct ggtcttttcc agcgtgcgga tgggggtgaa tccggaaatc
    15721 aacgccctgg caacattgat cctcggcgcg gtcggaattg tcggatttat cgcctggtat
    15781 ctgatggcgc gcgcagaaaa acagcggata cgcgacatcc aacgtgcaag acgtggctga
    15841 agacactaaa atttgccaac ctggctacat aatgccgcgc atgtcgcggc attgttttca
    15901 tggaagacga aacgttggga ttttttaaga aaacatcttc atctcatgct cgcctgaatg
    15961 tgcctgcgct ggtgcaggtg gcggcgctcg ccattattat gatccgtggc ctcgacgtgc
    16021 tgatgatttt caatacgctg ggcgtgcgcg gtattggcga gttcattcat cgcagcgtac
    16081 aaacctggag tttaacgctg gtctttttaa gcagtctggt gctggttttc attgagatct
    16141 ggtgtgcgtt ttcactggtg aaagggcgtc gctgggcgcg ctggctatat ctgctgacac
    16201 aaatcaccgc cgcaagttac ttgtgggcgg cttcgctggg gtacggttat ccggagctgt
    16261 tcagcattcc cggtgaatca aaacgtgaaa tcttccatag cctgatgctg cagaagctgc
    16321 cggatatgct catcctgatg ctgctgttcg ttccctcgac cagtcggcgg ttcttccagt
    16381 tgcaataatg tgtataatcg tcgcccctga tgatgtgaag gtcaatgtat gcagtgcgca
    16441 ctttacgacg cgggtcgctg tcgttcctgt cagtggataa cgcagccgat tccagagcaa
    16501 ctctccgcta aaaccgccga tcttaaaaat ctgctcgccg attttccggt tgaggaatgg
    16561 tgcgcgccgg tgtcaggccc ggaacaaggg tttcgtaata aagccaaaat ggtggtgagt
    16621 ggtagcgttg aaaaaacact gctcggtatg ctgcatcgcg atggcacgcc ggaagacctt
    16681 tgtgactgcc cgctttatcc agcctcattt gcgcccgttt ttgcggcgct aaaaccgttt
    16741 atcgcccgag cggggttaac gccctacaac gtggcgcgta agcgtggcga actgaaatac
    16801 attctgctga ctgaaagcca gagtgatggc ggcatgatgc tgcgctttgt actgcgttct
    16861 gataccaagc tggcgcaact gcgtaaggcg ctgccgtggt tacacgaaca actaccgcag
    16921 ctgaaagtta ttaccgtcaa tattcagccg gtacatatgg cgattatgga aggggagacg
    16981 gagatctacc tgaccgaaca acaggcactg gcggagcgtt ttaatgacgt accgctgtgg
    17041 atccgtccgc aaagtttctt ccagactaat ccggcggtcg ccagccagtt gtacgccacc
    17101 gcgcgcgact gggtacgaca gctgccggtt aaacatatgt gggatctgtt ctgcggtgtg
    17161 gggggctttg gtttacactg cgcgacgcct gacatgcagt taaccgggat cgaaattgcg
    17221 ccagaggcca ttgcctgtgc aaagcagtca gccgctgaac tgggcttaac tcgtttacaa
    17281 tttcaggcgc tggactccac tcagtttgcc actgctcagg gggaggtgcc ggagctggtg
    17341 ctggttaacc cgccgcgccg cggcattggt aaaccgctgt gtgattatct ttcaacgatg
    17401 gcaccgcgtt ttattatcta ctccagctgt aacgcccaaa ccatggcgaa agatatccgc
    17461 gaactgccag gttaccgcat cgaacgggta cagctttttg atatgttccc gcacaccgcg
    17521 cactatgaag tgttgacgct gctggtgaag caataaaaaa gccacaggtg cggcttcaga
    17581 ttgctgatca ggtgcgcttt gttcatgccg gatgcggcgt gaacgcctta tccggcctac
    17641 aaaatcatgc taaatcaata cattgcagag ttgatgtggg ccggataagc gcagcgcatc
    17701 aggcaatctt acttttgtca tcagtctcaa ttactgcgga aaccactggt cattgatttt
    17761 ctgatatgta ccgtcagctt taattgctgc cagcgcgtta ttcagttttt ccagcagagc
    17821 tttgttatcc ggacgtacag cgatacccag gccggtgccg aagtattgcg gatcggtcac
    17881 tttttcagtc gcaacgccca gttgcggatt ggttttcagc cattcgttta ccaccgcggt
    17941 gtcaccaaat accccatcaa tacgaccatt tttcagatcg ataaaggcat tctgataact
    18001 gtcataggag acagttttca cttccgggtg ctgatcctga atatatttct ggtgcgtcgt
    18061 accgttttcc atcccaatgc gtttgccttt caggtcggca aacgttttgt aggtatcttt
    18121 tttggaaatc acgacggctg agttttcata gtagggtgtg gtaaacgaaa cctgcttgct
    18181 acgctccggg gtgatatcca taccggagat tacggcgtca tattttctga atttcagtga
    18241 cgggatcagg ctgtcgaacg cgtgattagt aaaagtacat tctgcctgca tttgtttgca
    18301 caaggctttt gccagatcga tatcaaagcc gacaatctca ttattagcac ctatagattc
    18361 aaagggtgga taggtggctg aaacgccaaa attgattttc tctgcggcag aagcaccgaa
    18421 agtaaaggaa gcaagtaaag cggcaagaac taactttttc atgatggaac tcccgtctgt
    18481 caatcttatg atttttggcc gtgtctgcgg catgggataa caatgccatc aagtgaattt
    18541 atatgcaata aacatgatta aataatttaa atgaaataaa aaaagacgga taacttagtg
    18601 agttgtccgt cttcattata agaatttatg cactatgtag gccggataag gcgtccacgc
    18661 cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg ctggcgcgtc ttatcagacc
    18721 tacaaacccc cccggcgaat gtacgcagct acattaattc cgccgttcga atgccagtgc
    18781 tttgcgctcg atcagacgca tcatcagcgt cagcaggccg ttgacgacca agtaaataat
    18841 ccctgccgca ccgaacacca ttacatcgta ggtgcgtccg tacaacaact ggctgtatcc
    18901 catcacttcc atcagcgtaa tggtgtaagc cagagaggta cttttgaaca ccagcaccac
    18961 ttcgttggaa taagaagaga gcgagcgttt aaaggcatac ggcagcagga tcgccagcgt
    19021 atcttttttg ctcattccca gggcgctaca ggactgccac tgaccttctg ggatcgcacg
    19081 aattgcaccg taaaacagct gcgtggtata cgccgcactg ttcagcgata acgcaatcag
    19141 cgcacataac cacggttctg acaacaaatg ccacagtgcc ggatactcct gcaacgtcgg
    19201 aaactggccc ggcccgtaat aaatcaggaa gatctgcacc agcagcggcg taccggtaaa
    19261 cagcgtgata taaccccgca ccagccacac cagcaccggc gttttcagcg tcaggatgat
    19321 ggtaaaaatc aatgccagaa tcagtgccac aatcagcgag gcaacggtta gcgtcaggct
    19381 ggtgtgcagc cctttcatca gctcgggtaa atactcaaac attagctggg cctccgctca
    19441 aaacgtgtcg cgcgcaggtc aatgcgtttg agaatgtact gactgagcag ggtgatcacc
    19501 aggtaaatcg ccgccgccac aatgtaccag gtaaatggtt cctgggtacg agtagcaatg
    19561 cttttggttt gtaacattaa atcattcaca ctaatcaaac tgaccagcgc ggtatctttc
    19621 agcagcacca gccactggtt accgaggcca ggcagcgcat gacgccacat ctgcggcatc
    19681 accagacgga aaaagatagc cgatttcgac agccccagcg cctgaccaga ttcccactgg
    19741 cccaccggta cagctttcaa cgcgccgcgc agcgtttgcg aggcataggc ggcatacagc
    19801 agtgacagag cgatgacacc acaaaggaac ggactcacgt cgaagttctc aatgtccatc
    19861 tgcaccggga tctgcacgaa cccaagattg atagtgaagc catccgaaag cgtcagcagt
    19921 aactgcgagg agccaaaata gataaacagc actaccagaa tttctggcag gccacgcaga
    19981 atggttacca gcgctgaacc tgcccacgcg acaggacgcc atttcgctga ctcccatacc
    20041 gcaaagaaca tcgccagcgc cagcccgaca atcaatgcac aaacggcaag gccgacggtc
    20101 atcccggcgg cgcttgctaa aggaaaaaat tcattcatca ggaattactt ctggaaccat
    20161 ttgttgtaga tggtttcgta agtgccatct ttcttcactt tttccagcgc agtgttgagt
    20221 ttctgctgca gttcagtgtt tccctgacgt accgcgatgc cgaggccagt accgaaataa
    20281 tctttatcgg tcactttatc gcccactgcc gccagttttg ggttatcttt cagccactca
    20341 gtgaccactg cggtgtcacc aaatacgctg tcgatacgcc cgttttgcag atccagtttt
    20401 gcgttctggt agctgtcata cggaacggtg atgatttccg ggtgcttatc cataatgaac
    20461 ttctggtgtg tcgtcccgtt ctgaacgccg acttttttgc ctttcagctg atcaacactg
    20521 gtgtatttgc cttgctgacc cacaaacagg gcagagttgt catagtacgg ggtagtaaac
    20581 agtacctgct tttcacgctc cggagtgata tccatgcccg ccatcactgc ttctacgcga
    20641 cggaatttca ggcttgggat caggctgtca aacgcctggt tagagaaagt acaggttgca
    20701 tcaatctctt tacacagtgc ctgcgccagg tcgacgtcaa aaccaacgat ctggttgttt
    20761 gcatcaatcg attcaaacgg aggatagggg gcttcggtag caaaacgaat ggtttcggca
    20821 gctgtggcgg aaagactaaa acctgcaatt aacgcggcaa tcagaacttt tttcattgtt
    20881 gttatcccga atcttagtga gagagatagt ttttaaatgc ttcggtttgc ggctcggtaa
    20941 agcagctcgc gtcgccttgt tctacgatat gaccattttc catatacacc actcggctgg
    21001 cggttttacg cgccacttca acttcgtggg tgacgatcac ctgggtaata ttcgtttctg
    21061 ccagctcacg aatgatgctg acgatttgtg ccgtaatttc cgggtccagt gcggcggtcg
    21121 gttcatcgaa cagcagtacc tgcggttcca tcatcaacgc acgggcaata gcaacacgct
    21181 gctgctgacc accagaaaga tgcagcgggt aacgatcgct ataaggtttg agacgcagac
    21241 gttccagcag tttttctgca cgggccagcg cctgatcttt actcaacccc agtacacggc
    21301 agggcgcttc aatcaggttt tgctgcacgg tcagatgcgg ccacaggttg tattgctgaa
    21361 acaccatgcc aacgttacga cgcaaatcgc gaatcgcttt gtcagagggt gttttggtga
    21421 aatcgaaatg gttgcctgca atgttgagcg taccggagcg cggcatctca agcagattga
    21481 gtacacgcag cagcgagctt ttacccgcgc cgctggggcc aagtaacacc agcgtttcgc
    21541 cctgtgggca atccagcgtg atatcgaaca gcgcctgatg cgcgccgtag aagcaattaa
    21601 tgccgtttaa ttgaatactc attgacactc gtatactggc agtctgatag ctattgaggt
    21661 cgaagatagt acctttaaca gaataattat gcaatattta tgctttaaaa gttaaaagca
    21721 aagcgcatta ttcaataaac atagcacaaa ataacggggg cggtggtcgg tgagcataaa
    21781 tgtcggcatt cctcacgaaa tgccggataa tttacggggt ttattggttg atcaaggcgt
    21841 tagcgattct cgatggactg acggagcgta cccgccgtgg catgaacgct accgcctaag
    21901 tagcgcacat cgtcgatgac ccaacactgg ccttcctgaa tcattaacac ttcatcctgc
    21961 cagccctggt caccctgttt gagatccacc cgcaatggaa tgttacgggc atcacgatta
    22021 gggatagtcg atgcactggc aacgtgggcg ctgtctggca aggtggttcg actggagaac
    22081 ggatcgctgc ttaacaattc acgatgacta ttatcgcggc tggcatcgct aagttgggtt
    22141 gccagtttgt cactcaaata ggggcgcagg gcggtaatgt cattgctgcg atgttgaatg
    22201 cggtagtcgt agaattgttg agccacactg tcaggccctc cctggacaca ggaaccggtg
    22261 cgggggccgt tatctttata agctggcgtg acggtggtac aggcactcag cagcagtgca
    22321 catggaataa gtagagatag tgaacggtag cgcataatca tttccttatt agcttgctgt
    22381 ctgcgtcaca ctacgaccaa acgctatcag attatttaat acattgtttt taatacaatt
    22441 ctgaaacaat ctgaacacct gattgggttt gagtccattg ctggcgaccc actcaaggtt
    22501 gctcgtagct gacttactta aattacccaa acaatagaca gtgtaaacca tttgtgaggt
    22561 tgagcgtgtt atatggctga aatgatcatt taaggtaaat atgatagttc tgaaatggtg
    22621 gtataaatcc tcctgtagag gttaagagca tctgcaagaa gccagtttca catttataac
    22681 gcgtgataaa tgaatgcggt tattgtctaa tcattacaaa atttttcaca tgcgataaga
    22741 gatcctgcga ttaagatgat tgccaaaatt acgttgtatt tttgaaaatg agcgaaattt
    22801 gatagtccca gctttgactc tcgataatat tatttatgct acaattgtgg gtaaagaatt
    22861 taagctaatg aatttgagtg aagaataaat taaattgctt cagtatttcg ttaattaata
    22921 taaatacaaa aggcgttgtg cttttataat tataaagcgc gaggagttcg gtggattatc
    22981 gtttttcttg ggtgattttt aatatgataa acaatatatc tgatcaagca tcatcatttc
    23041 caggcacaca actaaatcaa agtgataatt tcctggattc actgagggaa tttttcgcga
    23101 tattaaatcc cagtcgtaag ggtgagttgt ctacatggga taccatctac ttgcatctga
    23161 tattagcaat aaatgctgac agtgatctca taaaaaatga tgtgttgctc gcggaaaata
    23221 ttccatcagc gaattaccaa tttaatacat tttttagcaa tactttcgag attgatgtta
    23281 aaaaatatct gggtaaatca gaagataacg aagttgaaat aaaagcgggt aatgaaagaa
    23341 tctctattgg aatacgtaat gtatcgaatg gaaaacttga aagacaacag tttcttttcc
    23401 cactggacta tgagaataaa ttacaagaac aattagataa gtattttacg attgaatcgc
    23461 acccattatt atacaggtat accattggta gtaagattgc gaatgtgatt tttgaaaagt
    23521 tatacagtcg tattgacttt aacaaagaac aatatatatc ctttataaag gacgcattta
    23581 ttcattttta tgattatagc aggcgatatg ctattagtga gaatatcgat aaagacgctg
    23641 taacaaacaa tatcgcattg atgagcactt tttatgacag tgataatacg tcaggtgagg
    23701 ttttaaataa tgattttaca gaagaagagt catttgaaac tgcgttagat gtagagcatg
    23761 caatagtgct tggatttgct gatgataatt ttgaaacaaa acctgttcat tatcaggatt
    23821 tattaacacg atttagtgcc tttcaagata ccgtgtttaa tctgtttcct gaaatgcact
    23881 cttcgcatta ccacgatatt tgtagcgtaa gtgtggatat gacaaaggga acgcaatgca
    23941 tgattcactt gatggtgaat gaagaagttt tcatgtctct gcctgttcca gttgccacga
    24001 tggtacgtga agatgcaagt aatttagtca atcttaaaac actactcaat gatggttgtt
    24061 tcattaaata tagtcatttt aatgacgtgg cattaataaa gcaaaacata agtaatctct
    24121 atttatcaca cactgttgtg aatgaaagca tattgaaaaa atgttgtttt gaaaacggca
    24181 gtttaggtga tgttaaaata acaaatagca atgtaataaa cagtgcattc aaaaatattt
    24241 cgtttagatc tgttaaaatt aataacgtaa atactcattc tttaaaattt attaactgca
    24301 attttttcaa tgttgatatg ataagagtta atctcagcaa atgtcttttt catgaatgta
    24361 gtatgcatgg tgtcaagata aaaccctggt tgcctgtaaa atggacaaaa gaattaataa
    24421 gtgattattt gtatggctgt ttactatctc tgtactctat ctgtgcaagg gatatatata
    24481 atatgaacgc cgggaataac gtgaaggttg ctgcggatgc tttccttgaa attattttct
    24541 ctcttaaaaa taaatattgt attaaactat taagcgctca ggatcgggcg tttatatacg
    24601 agtttgcaag aatgatcttc gcttatatta atgacaaaag cattgagatc ttattacttt
    24661 cttgttttgc ggcggctgac caaaaagcaa tccagcggta tagacctcag tcacaagatg
    24721 gggaagactt taggagtcat ttacaatata agttgcctct ttccgctcat taatgttgtt
    24781 ggtgcaacat aataagacag gtaagcggta ccctataatg aaaccaatat cgggttatgc
    24841 aacggataat cgtaaagact taagtgggtg tttttgacac aatgagatga tttcctaaac
    24901 tgatcaaaac cttcttccca acatggcata tcagtccgat aatgctttag agatcgaagg
    24961 cttaacaaac aaggagatcg atcatgcaat tttctacaac cccaactctg gaaggcctga
    25021 ccatcgttga atattgcggt gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc
    25081 gtgatttctt tgccggtatc cgcgatatcg ttggcggacg ttccggtgcg tatgaaaaag
    25141 aactgcgtaa agcacgggag atcgcctttg aggaattagg ctcccaggcg cgggcgctgg
    25201 gggccgatgc cgtagttggt attgatatcg actacgaaac ggtcgggcaa aacggcagta
    25261 tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg
    25321 gctggtcgct gccgctctgt tactggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa
    25381 agagggatat cagcttgata cccgacacca ggcgcaggcg gcgtatccgc gcattaaagt
    25441 gctggtgatc cactacaccg cagatgattt tgatagctcg ctggcgacac tgactgataa
    25501 gcaggtcagc tcgcattatc tgatccctgc ggtgccaccg cgatacaacg gtaaaccgcg
    25561 catctggcaa ctggtgccgg aacaagaact ggcctggcat gcggggatta gcgcctggcg
    25621 cggggcaacg cgccttaacg acacctctat tggcattgag ctggaaaacc gtggctggca
    25681 aaaatcatcc ggagtgaaat attttgcccc gtttgaacag gcacagattc aggcgcttat
    25741 tccgctggcg aaagatatta ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca
    25801 tgcggatatc gcaccgcagc gcaaagacga tccggggcca ttatttccct ggcagcaact
    25861 ggcgcagcag gggattggtg cctggccgga tgcgcagcgg gtgaactttt accttgccgg
    25921 gcgcgcgccg cacacaccgg tagataccgc gtcattgctg gagcttttgg cgcgctacgg
    25981 ttatgacgta aaacctgata tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca
    26041 gatgcatttc cgcccgacgt tatataacgg cgaagcggat gcagaaactc aggcgattgc
    26101 cgaagcattg ctggagaaat acgggcagga ttagcgcggc agttttccgt ggtcgcgtag
    26161 ccaggcggca gttttctcga tgccttcatc cagggtgatg accggctgat aacctaactc
    26221 ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc aacttggagg cgccgtagtg
    26281 ggtcagcggc ggctcttttg ctgacttgcg gcctaaacgc tccatgctgc gggcgatcat
    26341 atccagcatc gggtaaggga cggaacgaat acgacagtca atattcaact cgtcgatcag
    26401 cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg gtgatgttgt acacacgccc
    26461 ggaaggtagc ttatcgcagg cttcctggct tgccagccac attgcgtgca cggcattttc
    26521 atagtaggtc atatcgacca gcgcactgcc gccatgcggt aacagaatac tgccgtagtg
    26581 gtgcatcata tgcgccagac ggggaataaa gactttatcg tgcggtccga acagactttg
    26641 tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc gaaagcatat tgatcacttc
    26701 ttcgctggct gctttgctgc gggcaaactc gttggcgaag cggtgagggc gaaaatcttc
    26761 tttaatattg cgatggtggt gataatcgaa gtacagggag ggggaagaga tatgaataaa
    26821 gttacgcaca ccccaggcga cagcccattc acccaggcgg cgagtggcgc gaacgttagc
    26881 cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag ctggagcagt gccacagcgt
    26941 atcaatgccc gcgagcatca ctttagcttg tgatgaaacc agctcggtca gatccgtcgg
    27001 aacaaactct gcgcccattt tttccagcaa tttacccatt gcttcgttgc gaccgctcgc
    27061 acgcacgctg atgcctttct ggcataaaaa ctctaccgcg tttcgaccta agccgctggt
    27121 ggcgccggta accagtacct tcatatcaat ccactgttgt tgagaaaatg acgtgcgcat
    27181 tcttccgtga tttcccccat gatgcaatgg gaaacatgaa agaataacgc aggttttgtc
    27241 gattaatctg tgctttgttc tgccagtctg gcgatttgtt ttgccattcc gcgaaaaata
    27301 aacagatgcg cggggatcat caataaccag taaaacagcc ccggcatacc gtgcggatgc
    27361 cagaaagcgc ggacatcgat agtacgatag tcacctttat cttccagggt aaaacacagt
    27421 cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa gttgcttttt cggttcaacg
    27481 acaatcactt tccagctatc caccgcatcg ccggtctgta aatattcgcg ctccgggcgg
    27541 cctttcgcca gattatgacc gatcgcgcgg tccatcaacg cccgtgtctg ccacaaaata
    27601 ttgccaaaga aataacgctc tttaccgccg atttggttca ccaccagcca taaagccgca
    27661 aggctggcgg acgttttaac ggtaaacccc gcctgtttgg cgaaataacc gtactccggt
    27721 cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc tggagttgac cagtttttct
    27781 tcctctttca acgtgctacg taccgcgtca tcgaaagcga tcagccgttg tgggatgagt
    27841 gcacgtagcg cggtatcatc tgccagcaga tcgtgtttca gcccctgaat caacgccctg
    27901 gcggtggtgg gcggtacgga agtaatcaca ttgagaaacc acaccgaaat ccagcgggtg
    27961 gggaggggga tggggatcaa ccagcggcgc ttaccgctca ccgccataaa atgttcaaac
    28021 tgttgctgat aactgagcac ctctggtccg gcggcttcga agatgcggtg ttcgttggct
    28081 ggatggtcta acagcgccac cagatagtgc agcaagtttt ccagcgcgat gggcgtggtg
    28141 cgtgaacgta cccagcgtgg cggcgttaac actggcaggt tgtagaccat atcgcgcatg
    28201 acttcgaacg ccgctgaacc tgcgccaacg ataattccgg cccgaagttc ggtcactggt
    28261 acattcgctt cacgaagaat gtccgccgta gcctgacgag ctcgcagatg atccgactgc
    28321 tcatgtggcg gggcctgcaa cgaactgaga aagattaatt gcttaactgg tacttcacgt
    28381 agcgcatcgc ggacgttgag agccagctgg cgctcctgag cgataaaatc gccgccttcg
    28441 cccatgctgt gcaccagaaa atagacggta tcgatatcct gcaacagggc cggaaggtta
    28501 tccggccagc tgagatcgac tttatggcaa ctgacgtttg ccagttgcag ctttgcaagc
    28561 ctgtcgacat gacgtgccgc cgctaggatc tgatgccctt gctggctgag tgtgcgcacc
    28621 agatgctgac caatgtagcc actggcaccg agaactaaaa tgcgttgcgg cacgtctctc
    28681 tccttaacgc gccaggaatg cgcgccagtg ggcggcgact tccgccagtt gttcgcgcga
    28741 gacgtcaaga tgcgtcacca ggcggacaat cggcgaggcg ttaatcagca cgtttctcgc
    28801 tttcatgtat tcgcctaacg cggcagcatt ttcttccccg acgcgaacaa acagcatatt
    28861 agtgtcctga cgcatcacat ccgcgcctgc ttcacgcagt tgctccgcca tccaggcggc
    28921 gttgtcgtga tcttcctgca accgtgcgac gttattttcc agggcataca tcccggcggc
    28981 agccagaatc ccggactgac gcatcccgcc accggccatt ttccgccagc ggatggcacg
    29041 tttaatgtaa tcacgattac cgacgagtaa tgaaccgact ggcgtcccaa gacctttcga
    29101 caggcaaatg gtgaacgaat cacaatattg cgtgatctct ttcagttcgc agccgtaagc
    29161 caccacggca ttaaagatgc gcgcaccgtc aacatgcagc gccagattgc gctcgcgggt
    29221 aaattcccat gcttctttca ggtattcacg cggcagcact ttgccgttgt gggtgttttc
    29281 cagactgagt aatttggtgc gggcgaaatg gatatcgtcg ggtttgattt tcatcgccac
    29341 tttatccagc ggtagcgtgc cgtcggcagc cgcgtcgatg ggttgcggct gaatactgcc
    29401 cagcaccgct gcgccaccgg cttcaaacag atagttatgc gcggcctgac cgacaatata
    29461 ctcttcgcca cgttcgcagt gactgagcag agcgaccagg ttggcctgag tgccggtagg
    29521 cagaaaaatg gcggcttctt taccggaaag ctctgctgca tagtcctgca gagcattaac
    29581 ggtagggtcg tctccgtaaa cgtcgtcccc aaccggggcg gccatcatcg cttcgagcat
    29641 ggcgcggctc ggtcgggtaa cggtatcact gcgtaaatca atcatggcat gtccttatta
    29701 tgacgggaaa tgccaccctt tttaccttag ccagttcgtt ttcgccagtt cgatcacttc
    29761 atcgccgcgt ccgctgataa ttgcgcgcaa catatacagg ctaaaacctt tggcctgttc
    29821 gagtttgatc tgcggtggaa tggctaattc ttctttggcg accaccacat ccaccaacac
    29881 cggaccgtcg atagagaagg cgcgttgcag ggcttcatca acttcagagg ctttttctac
    29941 acggataccc gtaatgccgc acgcttcggc aatgcgggca aagttggtgt cgtgcagctc
    30001 agtaccgtct gtcaggtaac ctccggcctt catctccatc gccacaaagc ccagcacgct
    30061 gttattaaag acgataattt tcaccggcag tttcatctgc actactgaga ggaaatcgcc
    30121 catcaacatg ctaaaaccgc catcgccgca catggcgacc acctgacgtt ccggctcggt
    30181 tgcctgcgca cccaacgcct gcggcatggc gttagccatc gaaccgtggt taaacgaacc
    30241 taacaggcga cgcttgccgt tcattttcag ataacgtgcc gcccataccg ttggcgtgcc
    30301 gacgtcacag gtaaagatgg cgtcatcggc ggcaaaatga ctaatttgct gcgccagata
    30361 ttgcgggtga atggctttct cgctcggttt agctaaatcg tccagccctt tgcgggcatc
    30421 gcggtaatct tccagcgctt tatccagaaa cttgcgatcg gttttttctt ccaccagtgg
    30481 cagcaatgcg cgcagggttg atttgatatc gccgaccagc gccatatcta ccttgctatg
    30541 cgcgccgatg ctggctggat taatatcaat ctgaataatt ttggcatcgg tcgggtagaa
    30601 cgcacggtag gggaactgag taccgagtag cactaacgtg tcggcgttca tcatggtatg
    30661 gaaacctgac gagaatccga ttaacccggt cattccaaca tcatacggat tatcgtattc
    30721 gacatgctct ttaccgcgta gggcatgaac aataggtgct ttaattttcc cggcaaactc
    30781 aactaactct ttatgcgccc ccgcgcagcc gctgccacac atcagggcga tattgctgga
    30841 ataacgcagc agttgcgcca gtttgcgtaa ctcttcttct tccggcatca cgactggctg
    30901 tggcgcatga taccagtgcg tggttgcccc ttctggcgca ggttttaacg ccacgtcgcc
    30961 cggtaataca acaacggaaa cgccacggtt aagcaccgct ttgcgcatag caattgccag
    31021 cacttgtggg atctgctccg ggctggaaac cagctcgcaa tagtgactac attcgcggaa
    31081 tagctcttgt gggtgggttt cctggaaata gccgctgcca atttcgctgg agggaatatg
    31141 agcggcaatc gccagtaccg gaacgttatt gcggtggcaa tcgaataggc cgttgatcaa
    31201 gtgcaggttg cctgggccgc acgatccggc acagaccgcc agttctcctg aaagttgtgc
    31261 ttcagcgcca gcggcaaacg ccgccacttc ttcatggcgg gtggacatcc actcgatggt
    31321 gcccatgcga ttaagactgt cgctaagacc gttcagagag tcgcccgtga ctccccagat
    31381 gcgtttcacg cctgccgatt cgagtgtttt ggcgatataa gctgcaaccg tttgtttcat
    31441 ggttctccat ctcctgaatg tgataacggt aacaagttta gttcatctga cggaggggga
    31501 agggatggga gagaaaggag gcactaacgg ttaaatagcc cgatgaaagg aatatcatcg
    31561 ggcataaggc gattatgcga gaaccaaatc cccctgcgga tggcaggagc aggccagtac
    31621 gtaaccttca gcgatttcgg cgtcggtcag cgtcattgta ctgctcaccg tatattcacc
    31681 ggaaaccact tttgtcttac agcagccgca aacacccgca cggcaggcag cgacaaccgg
    31741 aacgttattg ctttccagcg cctccagtag cgtggtgcca accggggcgt aaaattctcg
    31801 tgccggttgc agtttggtga atttcagacc gctggtcgcc gcttctgcta ctggggtgaa
    31861 gaatttctct ttaaagaaac gcgtcacgcc gagcgctttc acttcctgct ctacccaatc
    31921 catatacgga gccgggccgc aggtcatcac ggtacgtgaa gctaagtcag gtacatctgc
    31981 cagcagttcg cgagtgaggc gaccagcgat aaagccttcg gtaacgttat tttctgccac
    32041 cagcgttacc ggatagttac gccactcatc ggcgaaaata acatcctgcg gcgtacgcac
    32101 gttgtagatc acccgcacat cggcctgtgg acggttcttc gcaagccagc gacgcatcga
    32161 cataatcggc gtgacgccgc agcctgccgc cagcaacagg aatttatctt ctgctttatc
    32221 gtcgcaggta aattccccca tcgcgtccga aagccagaga taatcaccgc gttttacatc
    32281 gcgcgtcagc cactgggagc cgacaccgtc atcaatccgc cgcacggtca gggtgatata
    32341 ttcactcacg cctggcgtgg aggaaatggt gtaagcacgc agcgtttccg ctgagtttcg
    32401 cacgctgacc agtgcatatt gcccggcgcg atatgggtag tgatcgtggc aaatcaggga
    32461 aatcgtccac acatccggcg tttcttgcgt aatgtgatga acctgtatcc gccacgggca
    32521 ttgattcgtt ggcatcgtca tcgacaaact ccttacgcgt tcaacagttg cttcatgtct
    32581 tcttcaacag tggtgataga acgcaggccg aatttctcgt tcagcaccgc cagcaggtct
    32641 ggtgtcagga aaccaggtgc agtcgggccg gtgacgatat ttttcacgcc cagagaaagc
    32701 agcgtcagca gaatgacaat cgctttctgt tcaaaccagg agagcaccag cgacagcggc
    32761 agatcgttca caccacagcc cagtttctct gccagagtga cagccagaat aattgctgag
    32821 taagcatcgt tacattgacc tgcatctacc agacgcggca gaccttcgat atcgccaaac
    32881 tcaagtttgt taaagcgata tttaccacag gcgagggtca ggatcaggca gtcatccggc
    32941 acgctggtgg cgaaatcggt gaagtagtgg cgctcgccgc gtgcgccgtc acagccacca
    33001 agcaggaaga tatgacgcag tttttcacgg ctaaccagat caatcagcgt atcggctgca
    33061 ccaagcagcg tctggcgacc aaagcccacg gtgatcaggt gaggaatttc gctgtacggg
    33121 aagcccgcca tttgttgcgc ctgggcgatg accgcagaga aatcttcgcc atccagatga
    33181 cgcacgccag gccagccaac gatgctgcgg gtccagatac gatcgtcata agcgcctacg
    33241 gttgggtcga tgatgcagtt cgaggtcatc acgatggggc cagggaaacg agcgaactcc
    33301 acttgctgat tctgccagcc gctgccgtag ttaccgacca gatgcttgaa tttacgcagc
    33361 tccgggtagc catgcgcagg cagcatttcg ccgtgggtgt agacattaac gcccgtgcct
    33421 tcggtctgtt ccagcaggtt gtagagatct ttgagatcgt gaccggaaat cagaatgcat
    33481 ttacccgccg tcgctttgac gttgacctgg gttggcgtcg ggtgaccgta tttaccggtt
    33541 tcgcctgcat ccagaatgct catcactttg aagttcatct ggccgatttc cattgaacac
    33601 tcaagaagcg cgttcatatc ggcaggccag gtccccagcc acgccatgat tttatggtac
    33661 tgggcataaa tatcgttgtc gtattgaccg agaacatgcg cgtgttccat ataggccgcc
    33721 gcacctttca ggccatacag gcacagcaga cgcaggccga gaatgttttc gccaatcgcc
    33781 gctttatctt tgttaggggt aaattctgct gcctgacgtt gcagctcgcc gagatcatcg
    33841 ctcaccagtt gcaggtcagc catcgggtta tcgacgcgcg cgttggcatc tacagccagg
    33901 cattgtgctt tcagcgcctc gcgcaaggca atcgcttcac gagcgtagcc gacaatacgc
    33961 ggagaatcga agttaacgtt ggtcagggtt gagaaaaagg cacgtggcgc gaagctgtca
    34021 acatcgtggt tgatgatgcc gtattcacgc gcttttaccg cccaggcaga aagcccttgc
    34081 agcgccgcga tgagtaaatc ctgaaggtca gaagtttccg ccgttttacc acacatcccc
    34141 tgcgcgtatg agcagccgtt ttctgccgga gtacggatag tttgttcaca ttgcacacaa
    34201 aacatgatca caccttttaa agttatattt aatatacatg tttaagggta agacgcttaa
    34261 cgcggggata aaagggattt ttcatgcaac tttaagggag attgatttag cgcaattttg
    34321 gcggcaggga actaccgcca gagaggtatt acgcagagaa aaaggcgatg aggatcggca
    34381 ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg gaccatatcc agcccgccag
    34441 tacgttgaag aacgggcagg gtgaaatcca ttgatgtggc accgcataag cccagtgcag
    34501 tagagcggct gcggcgaatc agcccaggga tcaacataat agcaatcagt tcacgggcca
    34561 gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa agattcggtc aataaaatac
    34621 cggaaagaga ataccagccg aaaccggagg ccattgccag cgcggtattg atggggagat
    34681 caagaataaa ggcgttaatt aaaccaccaa ttaatgaact gacaaccacc accacggcga
    34741 caatcattcc ccggcgatta aggacaatct gctttaaggt catgccatta ttgcgcaact
    34801 gaataccaac gaggaaaagt agcaaaatta acgtgtattc actggcttcg gtcgcgtgtt
    34861 gtaagaaagc cagtccactc agaccaatgg caaaaccaat cactactacg ccgcacagtt
    34921 ttagcgactc cagcgccatc gcaatacgcg acgggagttt ttcttgctga tggt
//
LOCUS       DAJTUP010000032        34550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTUP010000032 DAJTUP010000000
VERSION     DAJTUP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.032"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..334)
                     /locus_tag="OO468_001633"
     CDS             complement(<1..334)
                     /locus_tag="OO468_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258786.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="OO468_001634"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="OO468_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR6258787.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="OO468_001635"
     CDS             complement(1695..2075)
                     /locus_tag="OO468_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR6258788.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..2505)
                     /locus_tag="OO468_001636"
     CDS             complement(2089..2505)
                     /locus_tag="OO468_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258789.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO468_001637"
     CDS             complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO468_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR6258790.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3506..3973)
                     /locus_tag="OO468_001638"
     CDS             complement(3506..3973)
                     /locus_tag="OO468_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR6258791.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4138..5007
                     /gene="pldA"
                     /locus_tag="OO468_001639"
     CDS             4138..5007
                     /gene="pldA"
                     /locus_tag="OO468_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR6258792.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5140..6969
                     /gene="recQ"
                     /locus_tag="OO468_001640"
     CDS             5140..6969
                     /gene="recQ"
                     /locus_tag="OO468_001640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR6258793.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7033..7653
                     /gene="rhtC"
                     /locus_tag="OO468_001641"
     CDS             7033..7653
                     /gene="rhtC"
                     /locus_tag="OO468_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR6258794.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO468_001642"
     CDS             complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO468_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR6258795.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8446..9468
                     /gene="pldB"
                     /locus_tag="OO468_001643"
     CDS             8446..9468
                     /gene="pldB"
                     /locus_tag="OO468_001643"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR6258796.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9476..10276
                     /gene="yigL"
                     /locus_tag="OO468_001644"
     CDS             9476..10276
                     /gene="yigL"
                     /locus_tag="OO468_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR6258797.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10352..11251
                     /gene="bioP"
                     /locus_tag="OO468_001645"
     CDS             10352..11251
                     /gene="bioP"
                     /locus_tag="OO468_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR6258798.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO468_001646"
     CDS             complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO468_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR6258799.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12328..14589
                     /gene="metE"
                     /locus_tag="OO468_001647"
     CDS             12328..14589
                     /gene="metE"
                     /locus_tag="OO468_001647"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR6258800.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14628..15443)
                     /locus_tag="OO468_001648"
     CDS             complement(14628..15443)
                     /locus_tag="OO468_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR6258801.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15705..16466
                     /gene="udp"
                     /locus_tag="OO468_001649"
     CDS             15705..16466
                     /gene="udp"
                     /locus_tag="OO468_001649"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR6258802.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16607..18034
                     /gene="rmuC"
                     /locus_tag="OO468_001650"
     CDS             16607..18034
                     /gene="rmuC"
                     /locus_tag="OO468_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR6258803.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18129..18884
                     /gene="ubiE"
                     /locus_tag="OO468_001651"
     CDS             18129..18884
                     /gene="ubiE"
                     /locus_tag="OO468_001651"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR6258804.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO468_001652"
     CDS             18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO468_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR6258805.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19500..21140
                     /gene="ubiB"
                     /locus_tag="OO468_001653"
     CDS             19500..21140
                     /gene="ubiB"
                     /locus_tag="OO468_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR6258806.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21219..21488
                     /gene="tatA"
                     /locus_tag="OO468_001654"
     CDS             21219..21488
                     /gene="tatA"
                     /locus_tag="OO468_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR6258807.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21492..22007
                     /gene="tatB"
                     /locus_tag="OO468_001655"
     CDS             21492..22007
                     /gene="tatB"
                     /locus_tag="OO468_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR6258808.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22010..22807
                     /gene="tatC"
                     /locus_tag="OO468_001656"
     CDS             22010..22807
                     /gene="tatC"
                     /locus_tag="OO468_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR6258809.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKAEAESEKTEE"
     gene            22849..23631
                     /gene="tatD"
                     /locus_tag="OO468_001657"
     CDS             22849..23631
                     /gene="tatD"
                     /locus_tag="OO468_001657"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR6258810.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23628..24116)
                     /gene="rfaH"
                     /locus_tag="OO468_001658"
     CDS             complement(23628..24116)
                     /gene="rfaH"
                     /locus_tag="OO468_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR6258811.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24283..25776
                     /gene="ubiD"
                     /locus_tag="OO468_001659"
     CDS             24283..25776
                     /gene="ubiD"
                     /locus_tag="OO468_001659"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR6258812.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25822..26523
                     /gene="fre"
                     /locus_tag="OO468_001660"
     CDS             25822..26523
                     /gene="fre"
                     /locus_tag="OO468_001660"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR6258813.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26715..27878)
                     /gene="fadA"
                     /locus_tag="OO468_001661"
     CDS             complement(26715..27878)
                     /gene="fadA"
                     /locus_tag="OO468_001661"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR6258814.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27888..30077)
                     /gene="fadB"
                     /locus_tag="OO468_001662"
     CDS             complement(27888..30077)
                     /gene="fadB"
                     /locus_tag="OO468_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR6258815.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30267..31598
                     /gene="pepQ"
                     /locus_tag="OO468_001663"
     CDS             30267..31598
                     /gene="pepQ"
                     /locus_tag="OO468_001663"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR6258816.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31598..32212
                     /locus_tag="OO468_001664"
     CDS             31598..32212
                     /locus_tag="OO468_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR6258817.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32251..33702
                     /gene="trkH"
                     /locus_tag="OO468_001665"
     CDS             32251..33702
                     /gene="trkH"
                     /locus_tag="OO468_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR6258818.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33714..34259
                     /gene="hemG"
                     /locus_tag="OO468_001666"
     CDS             33714..34259
                     /gene="hemG"
                     /locus_tag="OO468_001666"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR6258819.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8145 a         8720 c         9327 g         8358 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtgatga tagagatgat
     2221 atatactgct aatgtaccaa aaacataaat ttttatatat atgaaaccac tatcacggag
     2281 tcgttggcaa ttcatgttga tgacgagata atggagtgcg atggtagaga ctataacaag
     2341 aaaacctgct tccccatcgt taaaaaagat aataagaaag gcaaaaatga aatttattaa
     2401 aataaataaa aatatatatc ggtgtctgga aatcttaccg ttagatgttg ggataaacat
     2461 acgtaacata aattttacat ccttgtgtga gtctcctgtc agcatggcaa tatgcccact
     2521 ctcatacaag agtcggcata ttttttcaga atatatttat tttttatttg gacgttctac
     2581 gctgcgtata aatcgcgtcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
     2641 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
     2701 gcgtcgggcc aatgtactga aaaaagccta acgttgagag acgcaggcgc gtggcagcgg
     2761 cagtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
     2821 gcgacatcgg gttttgcccc atatggctgg ttgaactgtc ggcaatagca aacaggtaaa
     2881 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     2941 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     3001 taggtagcga accaaaagtc cacagctgga ccaacacgcc acatatcgcc agaatcaccg
     3061 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     3121 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     3181 agataaacag tagccagttg ccaccaatca gtacggcaga gactgccagc ataaaaattt
     3241 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     3301 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     3361 agtaaatcag cttgaagtat gctggcgcaa taccccaaat aaaataagcg gcaagagcga
     3421 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     3481 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     3541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     3601 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     3661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     3721 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
     3781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
     3841 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt
     3901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     3961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     4021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     4081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     4141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     4201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     4261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     4321 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     4381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     4441 ttaggcccaa actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     4501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     4561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     4621 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     4681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     4741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     4801 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     4861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     4921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     4981 ggggttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     5041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     5101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     5161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     5221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggttgttatg
     5281 cccactggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     5341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actacaggcc
     5401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     5461 atgacaggct gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg
     5521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa
     5581 gcgcactgta tctcccaatg gggtcacgat ttccgcccgg aatatgccgc gctcggtcag
     5641 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     5701 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     5761 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     5821 atgcgctacg tgcaggaaca gcgcggtaag tctggcatta tctactgcaa cagccgggcg
     5881 aaagtagaag acaccgctgc gcgcctgcaa agcaggggta ttagcgcggc ggcctatcat
     5941 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgatctg
     6001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     6061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     6121 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     6181 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     6241 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     6301 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     6361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     6421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     6481 cgcgactatg gtcatgacaa actgaaagtc tatggtatgg gccgtgataa aagccatgaa
     6541 cattgggtga gcgtgatccg ccagctgatt catctcggcc tggtgacgca aaatattgcc
     6601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     6661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     6721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     6781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     6841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     6901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     6961 gaagagtagt cagcagcata aaaaagtgcc agtatgaaga ctccgtaaac gtttcccccg
     7021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     7081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     7141 gaagcgatga tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg
     7201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggta
     7261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     7321 gaggctgtat ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg
     7381 aaaggcttac tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc
     7441 tcattgtttg tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt
     7501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg
     7561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     7621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     7681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     7741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     7801 gtcctttaat ccatagagca atcctttgag caagggtggc gtaaccgatc atcacaataa
     7861 tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg
     7921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     7981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     8041 gatcaattgc gccagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     8101 ctgcccactt taacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     8161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     8221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     8281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     8341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     8401 cgcttaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     8461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     8521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     8581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     8641 agctacgtaa aatatgcgga actggcctac gacctgttcc atttggggtt tgatgtctta
     8701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     8761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt
     8821 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     8881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     8941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     9001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     9061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     9121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     9181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgcaaac gcttctctta
     9241 caagctgaag aggaacgcgt ggtggataac cgcatgcacg accgtttttg tgaactccgc
     9301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taataaaagg tgcttaccat
     9361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     9421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     9481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     9541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     9601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     9661 gattacctcc aatggtgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa
     9721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     9781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     9841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag ccgggattac tggagccgga
     9901 aggcatcagc aaagtgtttt tcacctgcga ttcccatgaa caactgctgc cgctggagca
     9961 ggcgattaac gctcgctggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
    10021 ggaagtgatg gcgggcggcg tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact
    10081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
    10141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagatctgca
    10201 tccggagctg gaagttattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
    10261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
    10321 tggatactcg ttaatcaaag agttttccat tgtggcgtta cttatcatca ccacgattct
    10381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
    10441 tgcggtgctg gtgcgcgttg gcttggcggc actcgttttt ctgccgtttc tgcgtacccg
    10501 tagcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
    10561 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat tgttgctgtt
    10621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
    10681 gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga ttattcgcta
    10741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
    10801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagta
    10861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
    10921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
    10981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
    11041 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
    11101 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
    11161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
    11221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
    11281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
    11341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
    11401 cgcgcggcaa ccatttgaat caacaataag gtgttatcga cgcttttcag tgacgggctg
    11461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
    11521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
    11581 tggtcaggtg ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca
    11641 ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc
    11701 tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg
    11761 aaatttccta aagcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc
    11821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg cggcagcacc
    11881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg ctggctctta
    11941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    12001 gattgcgtct gatgcaacgt tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct
    12061 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    12121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    12181 aaatcattcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    12241 cttcagtaag ctcccggcga tgaataaact tgccgccttc cctaaattca aaatccatag
    12301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    12361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    12421 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    12481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    12541 accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    12601 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    12661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    12721 aaaggccaac agttcaaact gacttggacg cagctgctgg aggaagtgga cgaggcgctg
    12781 gcgctgagcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    12841 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    12901 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    12961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    13021 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    13081 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    13141 aacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    13201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    13261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    13321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    13381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    13441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    13501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    13561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    13621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    13681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    13741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    13801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    13861 tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg
    13921 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    13981 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    14041 accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct ggaagccgct
    14101 ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    14161 agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    14221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    14281 gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    14341 gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc tggcgtctat
    14401 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    14461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    14521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    14581 cgggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    14641 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    14701 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtacacgata
    14761 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    14821 ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg
    14881 atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac
    14941 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    15001 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    15061 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    15121 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    15181 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    15241 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    15301 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    15361 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    15421 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    15481 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    15541 cctgcagaat gaagagtgat ttatgtgata tgaatcactt ttggtgggta aatttatgca
    15601 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    15661 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    15721 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    15781 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    15841 ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    15901 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    15961 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    16021 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    16081 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    16141 ctattggcgc gacaactcac gttggcgtga cagtttcttc tgataccttc tacccaggtc
    16201 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    16261 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaactctg ctgaccatgt
    16321 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    16381 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    16441 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    16501 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    16561 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    16621 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    16681 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    16741 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    16801 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    16861 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    16921 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    16981 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    17041 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    17101 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    17161 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    17221 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    17281 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    17341 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    17401 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    17461 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    17521 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    17581 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    17641 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    17701 ccgtaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    17761 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    17821 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    17881 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    17941 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    18001 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    18061 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    18121 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    18181 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    18241 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    18301 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    18361 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    18421 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    18481 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    18541 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    18601 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    18661 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    18721 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    18781 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    18841 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    18901 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    18961 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    19021 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    19081 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    19141 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    19201 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    19261 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    19321 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    19381 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    19441 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    19501 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    19561 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    19621 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    19681 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    19741 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    19801 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    19861 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    19921 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    19981 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    20041 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    20101 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    20161 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    20221 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    20281 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    20341 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    20401 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    20461 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    20521 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    20581 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    20641 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    20701 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    20761 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    20821 tcggtattcc tgctctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    20881 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    20941 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    21001 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    21061 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    21121 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    21181 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    21241 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    21301 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    21361 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    21421 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    21481 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    21541 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    21601 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    21661 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    21721 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    21781 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    21841 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    21901 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    21961 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    22021 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    22081 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcactt
    22141 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    22201 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    22261 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    22321 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcatt
    22381 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    22441 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    22501 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    22561 ctcgtcagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    22621 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    22681 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    22741 agaggaaaac gacgctgaag cagaaagcga aaaagctgaa gcagaaagcg aaaaaactga
    22801 agaataaatt caaccgcccg tcagggcggt tgtcatatgg agtacaggat gtttgatatc
    22861 ggcgttaatt tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct
    22921 tttgacgcgg gagttaatgg gctactcatc accggcacta acctgcgtga aagccagcag
    22981 gcgcaaaagc tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac
    23041 gacagcagcc agtggcaagc tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca
    23101 gaagtggtgg cgattggtga atgtggtctc gactttaacc gtaacttttc gacgccggaa
    23161 gagcaggaac gcgcttttgt tgctcagcta cgcattgccg cagaattaaa catgccggta
    23221 tttatgcact gtcgcgatgc ccacgagcgg tttatgacat tgctggagcc gtggctggat
    23281 aaactgcctg gtgcggttct tcattgcttt accggcacac gcgaagagat gcaggcgtgc
    23341 gtggcgcgtg gaatttatat cggcattacc ggttgggttt gcgatgaacg acgcgggctg
    23401 gagctgcggg aattgttgcc gttgattccg gcggagaaat tgctgatcga aacagatgcg
    23461 ccgtatctgc tcccgcgcga tctcacgcca aagccatcat cccggcgcaa cgagccagcc
    23521 catctgcccc atattttgca acgtattgcg cactggcgtg gagaagatgc cgcatggctg
    23581 gctgccacca cggatgccaa tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg
    23641 aactcggtgt tcttcacact gtgcttaatc tctttattaa taagattaag caatagcatg
    23701 gagcgagcct caccatcggg ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg
    23761 gtaataatca ccttatcacc cggataaggg ggggccggat cgacaatgtc tttcggttta
    23821 tataccgata gctgatgaat taccgccgat gggactatcg ctggcgacgc gccaaagcgc
    23881 acaaagtggc tgacgccacg cgtagcgctg atggtcgtgg tatgaatcac ttctggatca
    23941 aattccacaa acaggtagtt agggaataac ggttcactga ctgcggtacg tttaccccgt
    24001 acgatttttt ccagggtgat catcggagcg aggcaattaa ccgcctgtct ctcaaggtgt
    24061 tcctgggcac gttgaagttg cccgcgcttg cagtacagta aataccagga ttgcataatg
    24121 actcttatcc gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa gttaattata
    24181 ttccccggtt tgcgttatac cgtcagagtt cacgctaatt taacaaattt acagcattgc
    24241 aaagatgaac gccgtataat gggcgcagat taagaggcta caatggacgc catgaaatat
    24301 aacgatttac gcgacttcct gacgctgctt gaacagcagg gtgagctaaa acgtatcacg
    24361 ctcccggtgg atccgcatct ggaaatcact gaaattgctg accgcactct gcgtgctggt
    24421 gggcctgcgc tgttgttcga aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg
    24481 ttcggtacgc caaagcgcgt ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt
    24541 gaagttggta aattattggc gtttctgaaa gagccggagc cgccaaaagg tttccgcgac
    24601 ctgtttgata aactgccgca gtttaagcag gtgttaaaca tgccgacaaa gcgactgcgt
    24661 ggtgcgccct gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc
    24721 attatgacct gctggccgga agatgccgcg ccgctgatta cctgggggct gaccgtaacg
    24781 cgcggcccgc ataaagagcg gcagaatctg ggcatttatc gccagcagct gattggtaaa
    24841 aacaaactga ttatgcgctg gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg
    24901 tgtgcggcgc atccgggcga acgtttcccg gtttctgtgg cgctgggtgc cgatcctgcc
    24961 acgattctcg gtgcagtcac ccccgttccg gatacgcttt cagagtatgc gtttgccgga
    25021 ttgctacgcg gcaccaaaac cgaagtagta aagtgtattt ccaatgacct cgaagtgcca
    25081 gccagtgccg aaatcgtcct ggaagggtat atcgatcctg gtgagatggc gccggaaggg
    25141 ccgtatggtg accacacagg ttactataat gaagtcgata atttcccggt gtttaccgtg
    25201 acgcatatta cccagcgtga agatgcgatt taccattcca cctataccgg gcgtccgcca
    25261 gatgagcccg cggtactggg cgtggcgttg aacgaagtgt ttgtaccgat tctgcaaaaa
    25321 cagttcccgg aaattgtcga tttttacctg ccgccggaag gctgttctta tcgtctggcg
    25381 gtagtgacga tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg
    25441 tcgttcttac gccagtttat gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac
    25501 gcacgcgact ggaacgatgt gatttgggcg attaccaccc gtatggaccc ggcgcgggat
    25561 actgttctgg tagaaaatac gcctattgat tatctggatt ttgcctcgcc tgtctctggg
    25621 ctgggttcaa aaatggggct ggatgccacg aataaatggc cgggggaaac ccagcgtgaa
    25681 tggggacgtc ccatcaaaaa agatccagat gttgtcgcgc atattgacgc catctgggat
    25741 gaactggcta tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt gccctattta
    25801 tcgatccgac agagaaagcg catgacaacc ttaagctgta aagtgacctc ggtagcagct
    25861 atcacggata ccgtatatcg tgtccgcatc gtgccagacg cggccttttc ttttcgtgct
    25921 ggtcagtatt tgatggtagt gatggatgag cgcgacaaac gtccgttctc aatggcctcg
    25981 acgccggatg aaaaagggtt tatcgagctg catattggcg cttctgaaat caacctttac
    26041 gcgaaagcag tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga ccttccccac
    26101 ggagaagcgt ggctgcgcga tgatgaagag cgtccgatga ttttgattgc gggcggcacc
    26161 gggttctctt atgcccgctc gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat
    26221 atcaccattt actggggcgg gcgtgaagag cagcatctgt atgatctctg cgagcttgag
    26281 gcgctttcgt taaagcacct tggtctgcaa gtggtgccgg tggttgaaca accggaagca
    26341 ggctggcgtg ggcgtactgg taccgtgtta acggcggtat tgcaggatca tggtacgctg
    26401 gcagagcatg atatctatat tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg
    26461 ttttgcagtg agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc
    26521 tgagatataa aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa actttattca
    26581 gtggcattta gatctatgtc gcatccggca attggtgcac gatgcctgat gcgacgctgg
    26641 cgcgtcttat caggcctaca ttggtgccgg atcggtagac cggataaggc gttcacgccg
    26701 catccggcaa gtggttaaac ccgctcaaac accgtcgcaa taccctgacc cagaccgata
    26761 cacatcgtcg ccagaccaaa ctgaacgtct ttgcgttcca tcagattcag cagcgtggtg
    26821 ctgatacgcg caccggaaca acccagcgga tgacccagcg cgatcgcacc accattgagg
    26881 ttgatcttct cgtcaatctg ctccattagc ccgagatctt taatgcacgg caggatctgc
    26941 gcggcaaagg cttcgttcat ctcaaacacg ccgatatcgc tggcagaaag ccccgctttt
    27001 ttcagcgcca gcttcgaggc cggaaccggg ccgtaaccca taatcgatgg gtcacaacca
    27061 acgaccgcca tcgaacgcac acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt
    27121 tcactcatca ccagcatggc agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt
    27181 accgtaccgt ttactggatc aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt
    27241 tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga cgccgtcggc atcgtgacca
    27301 ccagttggga tgatttcatt tttaaatgcg gccgactgcg tggcggccca ggcgcgggcg
    27361 tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga taccgtgcat acgcgccagc
    27421 atttctgccg ttaagcccat catgcccgcc gctttggcga cattgcggct caggccgggg
    27481 tgaaaatcga cgccgtgact catcggcaca tggcctatat gctccacgcc gccaaccaga
    27541 catgcctgcg tatcgccagt catgatcatt cgtgctgcgt catgcagtgc ctgcatggat
    27601 gaaccacaca agcgattgac ggtaaccgcc gggacagagt gcggcacttc tgccagcagc
    27661 gccgcgttac gggcgatgtt aaaaccctgc tccagcgtct gttgcacaca accccagtaa
    27721 atatcgtcga gggccgccgc ttccagcgcc gggttacgcg ccagcaggct acgcattaaa
    27781 tgagcggaga gatcttccgc acgcacgtta cgaaaagcac cgcccttcga acggcccatc
    27841 ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg tgactcctta agccgttttc
    27901 aggtcgccaa ccggacgggc tggctcaacc ggaggatagt acggttcgtt atgacgcgct
    27961 ttattacgca gaccttccgg cacttcatac agcgggccga ggtgctgata ttgctgtgcc
    28021 atatcgaggt attttgcgct accgagggtg tccagccagc ggaacgcgcc gccgtggaac
    28081 ggagggaagc ccaggccgta gaccagcgcc atatccgctt ccgccggagt ggcgataatg
    28141 ccctcttcca gacagcgcac cacttcgttg accatcggga tcatcatgcg ggcgataatc
    28201 tcttcttcgc tgaaatcgcg cttcggttgg ctcacttctg ccagcaggtc ttcaacggcg
    28261 gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt tataacgcca gaaaccgagg
    28321 ccgttcttct gaccaaagcg gttggcatca aacagcgcgt cgatggcatc gcggtaatct
    28381 ttctgcatcc gctgcgggaa gcctgctgcc atgacagcct gagcgtgatg cgcggtatca
    28441 atgcccacaa cgtccagcag atatgccggg cccatcggcc agccaaactg tttttccatc
    28501 actttgtcga tcttgcggaa atccgcgccg tcgcgcagca gctggctgaa accggcgaaa
    28561 tacgggaaca gcacgcggtt aacaaagaag ccggggcagt cgttaaccac aatcggcgtc
    28621 ttgcccatct tgctcgccca ggcgacaact ttcgcgatgg tttcgtcgga gcttttctcg
    28681 ccgcgaataa tttctaccaa cggcattcgg tggaccgggt taaagaagtg catcccgcag
    28741 aagttttccg ggcgttccag cgcgttggcc agttcgctga taggaatggt tgaagtgtta
    28801 gacgccagca cggtatcctg gcgtactttt tgttcggttt ctgccagtac ggcttttttc
    28861 actttcgggt tttcaacaac cgcttctacc acaatatcca cgcggtcaaa tccggcgtag
    28921 tcgagcgttg ggtggatcgt ggagatcacg ccagccagtt tcagaccatc gattttgccg
    28981 cgctcaagct gcttgttcag cagtttcgcg gcttcggtca tgccgagggt taacgacttg
    29041 tcgttgatat ctttcatgac aaccggcacg cctttccacg cggactggta agcgataccg
    29101 ccacccatga tgcctgcgcc cagcacagca gcctgtttcg gggtttcaac gtctttggtg
    29161 agtttcttcg ctttgccttt tacatattga tcgttaagga aaatgccgac cagtgcgcga
    29221 gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt ccagatttaa ggcttcttca
    29281 cgaccaaaac gggccgcagc ttcaatggtt tttactgcgg tgatgggggc cggataatgt
    29341 ttccccgccg tttgcgcgac cattccttta gcgatggtga agctcatggt ggcttcaatc
    29401 ttgctcagtt ttagcggttc ctgcttcggc tgacgtttcg ctttccagtc gaggtcgccg
    29461 ttaatggcct ggcgtaaaat cgccatcgcc ccttcaatca gtttttctgc tttgactacg
    29521 ccatccacca gaccgatttt cagcgcctga tccgcgccga catctttacc ggcggcaatg
    29581 atttccagtg cactgtcagc gcccagcata cgtggcatac gtacagaacc gccaaagccc
    29641 ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca gatccggcgt cgccagacga
    29701 taatcggtcg ccagcacgca ttcgcagcca ccacccagcg catagccgtt gacggcagca
    29761 atggtcggca ccggcagatc ttccaggcga ttaaacacgc tattggcaaa gtgcagccac
    29821 tgacttaact gttcttcagg aacgaggaac agggacaaaa attcggtgat atcagcaccg
    29881 acgataaagg ctgctttgtt cgaacgcagc agcagccctt ttagatctga ttgctgttcc
    29941 agcacgccga tggcctcgcc gaggctggcg acggtcgcag tgtcgagttt attaactgaa
    30001 cctggggcat caaataccag ttcggcaatg ccatcttcca gccagtcaag gtacagggtg
    30061 tcgcctttgt aaagcatgtc agtctcctga atccgcaagg taatctggtc gtaccagatg
    30121 catcggagtg tgtgttttgt gttaaaaata tgcaaataaa agattaaaga aatgccgatc
    30181 tgatcacgct cggcagaaat cacgctctgg atgaacgatg tgctaagatg cggagactta
    30241 aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc ctcgctctat aaaaatcata
    30301 tagctacctt acaagaacgg actcgcgatg cgctggcgcg cttcaagctg gatgcattac
    30361 ttattcactc cggcgagctg ttcaacgttt ttctcgacga tcatccctat ccgtttaaag
    30421 tgaacccgca attcaaagcg tgggtgccgg taactcaggt gccaaactgc tggttgctgg
    30481 tggatggcgt gaacaagccg aaactgtggt tctatctgcc ggttgattac tggcacaacg
    30541 tcgaaccgct gccgacctcc ttctggactg aagatgtgga agtgatcgcg ctgccgaaag
    30601 ccgatggcat tggtagcctg ctgcctgctg cgcgcggcaa tatcggttat atcggtccgg
    30661 tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa catcaacccg aaaggggtga
    30721 tcgactacct gcattattac cgctccttca aaaccgagta cgaactggcc tgtatgcgtg
    30781 aagcgcagaa aatggcggtc aatggtcatc gtgcggcaga agaagcgttc cgttctggca
    30841 tgagcgagtt cgatatcaat attgcctatc tgaccgcgac cggtcatcgt gataccgacg
    30901 taccttacag caacattgtg gcgcttaacg aacacgctgc ggtgttgcat tacaccaaac
    30961 tggaccacca ggcaccggaa gagatgcgca gcttcctgct ggatgccggg gccgaatata
    31021 acggctatgc ggctgacctg acccgtacct ggtcggcaaa aagcgacaac gactacgcac
    31081 agctggtgaa agacgtaaat gatgaacaac tggcgctgat cgccaccatg aaagcaggcg
    31141 tcagctatgt ggattaccac atccagttcc atcagcgcat cgccaaactg ctgcgtaaac
    31201 atcaaatcat caccgatatg agtgaagaag cgatggtcga aaacgatctc accggaccgt
    31261 ttatgccgca cggtattggc catccgctgg gcctgcaggt gcatgacgtc gccggtttta
    31321 tgcaggatga tagcggtaca cacctcgcgg caccggcaaa atatccgtac ctgcgctaca
    31381 cccgtattct ccagccgggc atggtgttaa ccatcgaacc gggtatctac ttcattgaat
    31441 cgcttctggc accgtggcgt gaagggcagt tcagcaagca cttcaactgg cagaaaattg
    31501 aagcactgaa accgttcggc ggcattcgta tcgaagacaa cgtggtgatc cacgaaaaca
    31561 acgtggaaaa catgacccgg gatctgaaac tggcgtgatg gaaagctggt taattcctgc
    31621 ggcaccggtc acggtcgttg aagagatcaa aaagagccgc ttcataacga tgttggcgca
    31681 taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga
    31741 tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg gatgattctc aacagctggg
    31801 tttctctgac gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat
    31861 gggcagcggc gtcggggaaa ttaccgctgt ggtagtgcgc tactacggcg gcatattgct
    31921 tggcaccggt gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct
    31981 aacgacccaa cgcaagacgc cattaaccga atatactttg caatgtgaat atagtcagtt
    32041 aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa attatcaaca gtgattatca
    32101 ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa
    32161 gctggcggat tttagccgtg gttcattgca attgttagcg attgaagaat aatccccact
    32221 tcgttttgca gaactaagga agcggcagag atgcattttc gcgccattac ccgaatcgtt
    32281 ggactactgg tcatcttatt ttcagggacc atgattatcc ctgggctggt agcactcatc
    32341 taccgggatg gagcgggccg cgcttttacc cagacctttt ttgtcgccct cgccattggc
    32401 tctatgctgt ggtggccgaa ccgcaaagag aagggcgaac tgaaatcccg tgaggggttt
    32461 ctgatagtgg tgctgttttg gaccgtgctg ggtagtgtcg gtgcgctccc ttttatcttc
    32521 tcggaaagcc cgaacctcac gattaccgat gcgttttttg aatctttctc tggcctgacc
    32581 accaccgggg ccactacgct ggtggggctg gattcgctcc ctcatgccat cctcttttat
    32641 cgccagatgc tgcaatggtt tggcgggatg gggatcatcg tgttagcggt tgcgatactg
    32701 cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa
    32761 gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa ctctgtggtt gatttatgtc
    32821 ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg gaatggatgc ctttgatgcc
    32881 atcggccata gctttgcgac tatcgctatt ggcggcttct cgacacatga tgccagtatc
    32941 ggttatttcg acagtccgac tattaacact atcattgcta tcttcctgct gatctccggc
    33001 tgtaactacg gtctgcactt ttcactgtta agtgggcgta gtctgaaggt ttattggcgc
    33061 gatccggaat ttcgcatgtt tatcggcgta cagtttacgc tggtggttat ttgtacactc
    33121 gtactgtggt ttcataatgt ctacagttcg gcgctgatga caattaacca ggcgtttttc
    33181 caggtggtat cgatggcgac aaccgccggg tttacgactg acagcattgc ccgctggccg
    33241 ctctttttgc cggtactgct tttatgttca gcttttatcg gcggttgcgc cgggtcaacg
    33301 ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg
    33361 aaacgactgg tgcatccgaa cgccgtctat agcattaagc tggggaatcg cgcactgccg
    33421 gaacgtatcc tcgaagccgt ttggggattt ttctccgcct atgcattggt gtttattgtc
    33481 agtatgctgg cgattatcgc cacgggcgtg gatgattttt ctgcctttgc ctcagttgtt
    33541 gcgacattga ataacctggg gccagggctt ggcgtggttg ctgataactt taccagtatg
    33601 aacccggtgg ctaaatggat cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt
    33661 acattactgg tgctctttac cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa
    33721 cattaattct tttctcaaca agggacggac aaacgcgcga gattgcctcc tacctggctt
    33781 cggaactgaa agaactgggg atccagacgg atgtcgccaa tgtgcaccgc attgaagaac
    33841 cacaatggga aaactatgac cgtgtggtca ttggtgcttc tattcgctat ggtcactacc
    33901 actctgcgtt ccaggaattt gtcaaaaaac atgcgacgcg actgaattcg atgccgagcg
    33961 ccttttattc cgtaaacctg gtggcgcgca aaccggagaa gcgtactcca cagaccaaca
    34021 gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg
    34081 ccggggcgct gcgttaccca cgttatcgct ggtacgaccg ttttatgctc aagctgatta
    34141 tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt tgtctatacc gattgggagc
    34201 aggtggcgaa tttcgcccga gaaatcgccc atttaaccga caaaccgacg ctgaaataag
    34261 cataaagaat aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg
    34321 aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg cagccgggtc
    34381 agcggggttc tcctgagaat tccgacagag aaagcaaaaa taaatgcttg actttgtagc
    34441 gggaaagcgt attatgaaca ccccgcgccg ctgagaaaaa gcgaagcggc actgctcttt
    34501 aacaatttat cagacaatct gtgtgggcac tcgaagatac ggattcttaa
//
LOCUS       DAJTUP010000033        33797 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTUP010000033 DAJTUP010000000
VERSION     DAJTUP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33797
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.033"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1984
                     /locus_tag="OO468_001667"
     CDS             <1..1984
                     /locus_tag="OO468_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR6258820.1"
                     /translation="RTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPV
                     MVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTEL
                     VPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADD
                     VSAFELGNNWTADLGRVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSD
                     GDACTTDSRESYNALSCYESISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFE
                     NNYLRQTTAQSISETVQLSASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSL
                     SDTPTMDSNNNSHSTNVPTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNT
                     DTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASG
                     TDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVE
                     VNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFP
                     IVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTC
                     SVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            1981..3069
                     /locus_tag="OO468_001668"
     CDS             1981..3069
                     /locus_tag="OO468_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR6258821.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3157..4455)
                     /gene="exuT"
                     /locus_tag="OO468_001669"
     CDS             complement(3157..4455)
                     /gene="exuT"
                     /locus_tag="OO468_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR6258822.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            4940..6352
                     /gene="uxaC"
                     /locus_tag="OO468_001670"
     CDS             4940..6352
                     /gene="uxaC"
                     /locus_tag="OO468_001670"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR6258823.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6367..7854
                     /gene="uxaA"
                     /locus_tag="OO468_001671"
     CDS             6367..7854
                     /gene="uxaA"
                     /locus_tag="OO468_001671"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR6258824.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILSTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            7937..8488
                     /locus_tag="OO468_001672"
     CDS             7937..8488
                     /locus_tag="OO468_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR6258825.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8493..9737)
                     /gene="sstT"
                     /locus_tag="OO468_001673"
     CDS             complement(8493..9737)
                     /gene="sstT"
                     /locus_tag="OO468_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR6258826.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10333..11298)
                     /gene="alx"
                     /locus_tag="OO468_001674"
     CDS             complement(10333..11298)
                     /gene="alx"
                     /locus_tag="OO468_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR6258827.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11569..12567)
                     /locus_tag="OO468_001675"
     CDS             complement(11569..12567)
                     /locus_tag="OO468_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6258828.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12646..13338)
                     /locus_tag="OO468_001676"
     CDS             complement(12646..13338)
                     /locus_tag="OO468_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR6258829.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13415..13918)
                     /locus_tag="OO468_001677"
     CDS             complement(13415..13918)
                     /locus_tag="OO468_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR6258830.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14003..15139
                     /gene="rlmG"
                     /locus_tag="OO468_001678"
     CDS             14003..15139
                     /gene="rlmG"
                     /locus_tag="OO468_001678"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR6258831.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15424..15738
                     /gene="higB"
                     /locus_tag="OO468_001679"
     CDS             15424..15738
                     /gene="higB"
                     /locus_tag="OO468_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR6258832.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15735..16151
                     /gene="higA"
                     /locus_tag="OO468_001680"
     CDS             15735..16151
                     /gene="higA"
                     /locus_tag="OO468_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR6258833.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16196..18214)
                     /gene="fadH"
                     /locus_tag="OO468_001681"
     CDS             complement(16196..18214)
                     /gene="fadH"
                     /locus_tag="OO468_001681"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR6258834.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18440..20791)
                     /gene="ygjK"
                     /locus_tag="OO468_001682"
     CDS             complement(18440..20791)
                     /gene="ygjK"
                     /locus_tag="OO468_001682"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR6258835.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20808..21227)
                     /locus_tag="OO468_001683"
     CDS             complement(20808..21227)
                     /locus_tag="OO468_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258836.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21205..21558)
                     /locus_tag="OO468_001684"
     CDS             complement(21205..21558)
                     /locus_tag="OO468_001684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258837.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21559..21879)
                     /locus_tag="OO468_001685"
     CDS             complement(21559..21879)
                     /locus_tag="OO468_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258838.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22013..23446)
                     /locus_tag="OO468_001686"
     CDS             complement(22013..23446)
                     /locus_tag="OO468_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR6258839.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23509..23958)
                     /locus_tag="OO468_001687"
     CDS             complement(23509..23958)
                     /locus_tag="OO468_001687"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR6258840.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(23955..27041)
                     /gene="ebgA"
                     /locus_tag="OO468_001688"
     CDS             complement(23955..27041)
                     /gene="ebgA"
                     /locus_tag="OO468_001688"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR6258841.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27224..28207)
                     /gene="ebgR"
                     /locus_tag="OO468_001689"
     CDS             complement(27224..28207)
                     /gene="ebgR"
                     /locus_tag="OO468_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR6258842.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28425..28756
                     /locus_tag="OO468_001690"
                     /pseudo
     CDS             28425..28756
                     /locus_tag="OO468_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28798..30177)
                     /gene="ygjG"
                     /locus_tag="OO468_001691"
     CDS             complement(28798..30177)
                     /gene="ygjG"
                     /locus_tag="OO468_001691"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR6258843.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30595..32115
                     /gene="aer"
                     /locus_tag="OO468_001692"
                     /pseudo
     CDS             30595..32115
                     /gene="aer"
                     /locus_tag="OO468_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32269..32892)
                     /gene="nfeR"
                     /locus_tag="OO468_001693"
                     /gene_synonym="yqjI"
     CDS             complement(32269..32892)
                     /gene="nfeR"
                     /locus_tag="OO468_001693"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR6258844.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33169..33797
                     /gene="nfeF"
                     /locus_tag="OO468_001694"
                     /pseudo
     CDS             33169..33797
                     /gene="nfeF"
                     /locus_tag="OO468_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
BASE COUNT         8235 a         8726 c         8871 g         7965 t
ORIGIN      
        1 ccgcacacta tttaatgcac aaggcgggaa ctttaccttt aactttctgc cactcggtgc
       61 aatcgatggg atgcgtatcg ggtcgacact gagctattta aaccaggcgc aaagccagca
      121 aggaacccca gtaatggttc tgctttcgcg caattctcgt gttgacgctt atcgtaatga
      181 gcaacttttg ggatcgtttt atctcaatag tggttcgcaa tttattgata ccagttcctt
      241 tccgccaggc agctacagcg tggcattaaa agtctacgaa aataaccaac tcacccgcac
      301 cgagcttgtg ccgtttacca aaaccggcgg tctgactgac ggaaatgcgc aatggttctt
      361 acaggcaggt aaaactacat cacaggtttc tgatgatgaa agttcagctt atcagctagg
      421 agtacgcctg ccattacatc cgcaatatga gctctacgca gggctggcga atgctgatga
      481 tgtgagtgct ttcgagttag gtaataactg gacggcagat ttaggcaggg tggggaatct
      541 tgcaatcagc gccagcgtgt tccgtaacga tgacggcggc aaaggtgata tgcaacaggc
      601 caactggagt aatccgggat ggccgacgtt gggcttttat cggaccaact ctgacggcga
      661 tgcttgtaca accgacagca gagagagcta taacgcctta agctgttatg aaagtatttc
      721 cgcgacagtt tcactgaatt ttgtcggctg gaatatgatg ctgggttata cctgcacaca
      781 aaataacact gatgatagtt tgcgttggga taaacagcag agctttgaaa ataactatct
      841 tcgccagaca acagcgcaaa gtatctctga aactgtacaa cttagcgctt cccgcgctat
      901 tgtgatgcgt gactggattt tgagtacgtc agtcggtgtt ttccatcgta atgacaacgg
      961 tggcgataac gacgacaacg gcttgtactt atcgttttcg ttatctgaca cgccaacgat
     1021 ggatagcaat aacaacagcc attcaaccaa tgtgcctacg gattatcgtt atagcgaaca
     1081 ggatggcgat caaacgtcat ggcagttatc gcataccttt tataacgatt cattcagcca
     1141 taaagaactt ggcgtaaccg tcggaggcct gaacaccgat accataaaca gcgcggttaa
     1201 cgggcgttgg gatgggcaat acggaaatgt ctacgctacc gtatctgata gttatgaccg
     1261 taagaatcat gatcatcttt cggcctttac gggcacttat agctctacgc tggctgtcag
     1321 tcgctatggc gttaatttgg gagccagtgg tacagacgat ttgctgggtg cggtactggt
     1381 ggatgtgaaa ggcttctctg aacaggatga agagagtcag gatctgcaac tcgaagcgcg
     1441 ggtggcaggc agccgaactt tgcagcttgg tcaaagtgac agtgtgttgt tcccttatcc
     1501 tggatttcag tctggttttg ttgaggttaa cgacagtagc cagggcaatc agcaggggac
     1561 aacaaacatc attaacggtg cagggaatcg tgaattaatg ttgttgcctg gcaagctgcg
     1621 ctatcgcgaa gtgtctgcca gctttaacta caactatatc ggtcgtttat tactgccggc
     1681 agcggtgaaa aaattcccga ttgtggggct gaacagcgcc atgttactgg tagctgaaga
     1741 tggcggattt acacttgaaa ttaacggcag cgaaaaagag ctctatctgc tttccgggca
     1801 gcaattcctt aagtgtccac tgagtgttgt aaagaaacgc gccagcattc gttacagcgg
     1861 agatgttact tgtagtgtgg tgacttattc acaattaccg gagtcgattc aggttcaggc
     1921 acagttgaaa cagcctaaat tacgtggaaa cgttcagacg gcgcaaaggg aggttgcacc
     1981 atgagaaatc gattgattgc ggcgatattg ggtttgtttg gcacgctcac tggcgttcag
     2041 gcagctcctg acgtgaccag tgaaattacg tatgatttgg catctggtag agcggattat
     2101 tacttctgga aggatgaagc gtcggcagga aataatggat atatgtggta tgaatgttcg
     2161 tatcctgacc tccaacaaac ctgtacagct aatggaaata tatcgacagt acaaatctat
     2221 ttaactgaac aacgcagtgg gatgcgttgg ccggtaaaac tcaaaggatt taaaacagcc
     2281 attgtaagta gcgatgaagc gccgctagga tgcaaggggg gcaaagggct tcagacgaat
     2341 cttaaggatt ctaatagatc ttcatgtaca gaagatggtc aacattatta tatatacgat
     2401 acaaagtttc ttacgctcta ccttgagcag acagagatga agaatttgcc gattggtggc
     2461 gtctggaagg ggaaagttaa attacattcg aacagcccgg cccaggacta tttcgcaaat
     2521 attaccctga atacgctcga ccccaaccat attgacgtgt tcttcccgga gttcgcccac
     2581 gccacgccaa gggtgcagtt agacttgcat ccaacaggaa gcgttaacgg cagcaactac
     2641 gcgcaagatc tgaccatgtt ggacatgtgc ctgtacgatg gttttaacgg taatgccatc
     2701 agttatgaaa tcatgctcaa agatgaaggg cgaccagctg cagggcgcag agacggttac
     2761 ttctctatct atcgtcaggg agggaccacc accgacgagg gagaacgcat tgattaccgg
     2821 gtcaaaatgt acaacccgga aaccggtggg caaattgatg tgcgcaataa tgaaaatatg
     2881 gtctggaaca gcattaacct gaaacgtgtg cgtccggtgg tactgcccgg tattcgctat
     2941 gccgtgatgt gtgtgccgac acccttgact ctggcagtcg ataaattcag cgtgatggat
     3001 aaacaggccg ggtactacat gggcaaattg tcagtaatct ttacgccttc cttgccaacc
     3061 atcaattaac cctgcaaacc ggacaaacgc ttttttctat ataaacgacg aagccgcctg
     3121 ataaggcggc ttcgtcgtta taacttacgt taaacgttaa tgttgcggtg cgggatcgtt
     3181 atgtgtttcc tgcgccacct caatcgccgg tttgttctgc aatacggtcc agataaccag
     3241 cgcacccagc aggtcaaata ctgccagaac tgcgaacagc gggctgaagc cgatggtgtc
     3301 agccagtgca ccgacaacca gcgcaaacag cgtacttgcc agccatgcgg acatcccggt
     3361 taaaccgttc gccgtcgcca cttcgttacg accgaacacg tcagaagaga gcgtaatcag
     3421 cgcgccagac agtgcctggt gggcaaaacc accgatacac agcagcataa ttgcgacata
     3481 cgggttggtg aacagaccga tcatacccgg gccaatcatc agcacagcac ccagcgttac
     3541 gaccatctta cgggaaacga tcaggttcac accaaaccaa cgctggaaca gcggcggcag
     3601 gtaaccaccg aggatacaac cgaggtcagc aaacagcatc ggcatccagg cgaacatcgc
     3661 gatctctttc aggttaaagc cgtaaacttt aaacatgaac agcgggatcc acgcgttaaa
     3721 agtaccccag gccggttctg caaggaaacg cggcagtgcg ataccccaga actgacggtt
     3781 acgcaggatc tgaccaacgg acattttctt cgccgtgctc acctggtgct gggcttcctg
     3841 accattaata atatagtcgc gttcttcatc ggtcagatgc ttctggtcgc gcggatgttt
     3901 atagaaaatc agccatgcca tcgcccagat aaagctcaat gcaccggaga tgataaatgc
     3961 catctgccag ctgtgcatca cgattgccca taccaccagc ggcggcgcaa tcatcgcacc
     4021 aatcgaagaa cctacgttaa agtagcctac tgcgatggaa cgctctttcg ccgggaacca
     4081 ttcggagctg gctttcagac ccgccgggat catcgctgct tccgcggcac cgaccgcacc
     4141 acgagcaaca gctaggccac cccagctacc tgccagcgcg gttgcaccac agaacacggc
     4201 ccacagcaca gcaaacattg cataaccgat tttcgtaccc agcacatcca gtacataacc
     4261 tgctaccggt tgcatgaccg tataagcagc agaataggct gcgatgatat aggaatactg
     4321 ttgggtggag atgtttaact cttccatcag agttggcgca gctgccgcca cagtgttacg
     4381 cgtcaggtaa ccaagcacgg tgccgagcgt caccagtgcg atcatatacc aacgtaaccc
     4441 tttaatttta cgcatgtaaa acctcatcgt tttgttatat ccgcaggaag cgcggccatc
     4501 atcccgccag aggctgaaga tgttagttac gaccgggtaa ccgaaaaaaa aaaccggcac
     4561 gccccgaacg ttgccatgaa agtttcgtgc taattcggct tccgtaccgg ttaacccgat
     4621 tcatctaaca aatgaatgaa tcggcaattt gcattccgtc gacttatgat ttgcgacggc
     4681 agaaagataa cttgtcatac aaccttaaaa ggtgagagcc atcacaaatg tgcgaatatt
     4741 tgtagggaca ttacctgacg acagcgaggc cagtactggc gcggcctgca gcgagattta
     4801 ccactttgag agtaattttt ttaactacgt ttattgatct aactcacgaa aatatcttcg
     4861 gactctggaa attggtgtga taactttgtc agcatcgcac cataagcaag ctagctcact
     4921 cgttgagagg aagacgaaaa tgactccgtt tatgactgaa gatttcctgt tagataccga
     4981 atttgcccgc cgtctgtatc acgactacgc aaaagaccag ccgattttcg attaccattg
     5041 ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt aaaaacctgt atgacatctg
     5101 gttgaagggc gatcactaca aatggcgcgc tatgcgtacc aacggtgtgg ctgagcgtct
     5161 gtgtactggt gatgcgtctg accgtgaaaa atttgacgcc tgggcggcga ctgttccgca
     5221 cactatcggc aacccgttat accactggac gcacctcgaa ctgcgtcgtc cgttcggtat
     5281 cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc tggaacgaat gtaacgaact
     5341 gctggcgcag gataacttct ccgcgcgcgg catcatgcag cagatgaacg tgaaaatggt
     5401 cggcaccacc gatgacccga tcgattctct ggagcatcac gcagagatcg ccaaagacgg
     5461 ctctttcacc atcaaagtgc tgccgagctg gcgtccggac aaagccttca atatcgaaca
     5521 ggcgaccttt aacgactaca tggcgaagct gggcgaagtt tccgataccg acattcgccg
     5581 ctttgctgac ctgcaaactg ccttgaccaa acgtctggat cacttcgccg ctcacggctg
     5641 taaagtgtct gaccatgcgc tggacgtggt gatgtttgct gaagcgaacg aagcggaact
     5701 ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg agcgagcacg aagtggcaca
     5761 gttcaaaact gcggtactgg tgttccttgg tgccgaatat gcacgtcgcg gctgggtaca
     5821 gcagtaccac attggcgcgc tgcgtaataa caacctgcgt cagttcaaac tgctggggcc
     5881 ggatgtaggc tttgactcca tcaacgaccg tccgatggcg gaagagttgt ctaagctgct
     5941 gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt ctctactgcc tgaacccgcg
     6001 cgataacgaa gtgctgggca ccatgatcgg taacttccag ggcgaaggta tgccgggcaa
     6061 aatgcagttc ggttccggct ggtggtttaa cgatcagaaa gacggtatgg aacgtcagat
     6121 gacccaactg gcgcagctcg gtctgctgag ccgctttgtc ggtatgctga ctgacagccg
     6181 tagcttcctg tcatacaccc gtcacgaata cttccgccgc attctgtgcc agatgatcgg
     6241 tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac ctgctgggcg agatggtgaa
     6301 aaatatttgc tttaacaatg cgcgtgacta cttcgccatt gaactgaact aaggtctggg
     6361 gttgatatgc aatacatcaa gatccatgcg ctggataacg tcgcggtcgc tttagcagat
     6421 ttggctgaag gcacagaagt cagtgtcgat aaccagactg ttacgctgcg ccaggatgtt
     6481 gctcgtggac ataaatttgc gttaacggat atcgcaaaag gggccaacgt catcaaatat
     6541 ggcctgccga ttggttatgc attggcggat attgcggcgg gggaacacgt ccacgcccac
     6601 aatacgcgca cgaatctgag cgatctggat cagtatcgct atcaacttga ttttcaggat
     6661 ctgcctgcgc aagcggcaga tcctgaagtg cagatctatc gtcgcgctaa cggcgatgtc
     6721 ggggtgcgta acgagctgtg gatcctgtca accgtgggct gtgtcaacgg catcgcgcgg
     6781 caaatccaga accgtttcct gaaagagacc aacaacgccg aaggtaccga cggcgtgttc
     6841 ctcttcagcc acacctatgg ctgctcacaa ctgggcgacg atcacatcaa cacccgcacc
     6901 atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag tgctggtgat tggtctgggc
     6961 tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg gcgatatcga tcctgaacgc
     7021 gttcatttca tgatctgcca acagcaggat gatgagatcg aagccgggat cgagcatttg
     7081 catcagctgt ataacgtgat gcgcaacgat aaacgcgagc caggcaaact cagcgaactg
     7141 aagtttggtc tggagtgcgg tggttctgac ggtctttctg gtattactgc taacccgatg
     7201 ctggggcgtt tctctgacta cgtgattgct aacggcggta ctaccgtact gaccgaagtg
     7261 ccggagatgt ttggcgcaga gcagttgctg atggaccatt gccgcgacga agcaacgttt
     7321 gaaaaactgg tcaccatggt caacgacttc aaacagtact ttattgccca tgatcagccg
     7381 atctacgaaa acccatcacc ggggaacaaa gcgggcggta tcaccacgct ggaagacaaa
     7441 tcacttggct gtacccagaa agcgggttcc agtgtcgtgg ttgacgtgct gcgttacggc
     7501 gagcgtctga aaacgccagg gctgaacttg ttaagtgcgc cgggtaacga tgccgtagcg
     7561 accagcgccc tggcgggtgc gggctgccat atggtgctgt tcagtactgg tcgcggcacg
     7621 ccatacggtg gatttgtgcc gacggtgaaa atcgccacca acagtgaact ggcggcgaag
     7681 aaaaaacact ggatcgactt tgacgcgggt cagctgatcc acggtaaagc gatgccgcag
     7741 ttgctggaag aatttatcga caccatcgtt gagtttgcca acggtaagca aacctgcaac
     7801 gagcgtaacg acttccgcga actggcgatc ttcaaaagcg gcgtaacgct ataaagtcga
     7861 aatgagttgt taataaaaaa cggcgtttca taatgtgaag cgccgtttgc ttttccatac
     7921 ctgctaagga tttatcatga ccgcgtattg gctggcccag ggcgtgggtg tcatcgcctt
     7981 tctgattggt atcacaacat ttttcaatcg tgacgaacgt cgcttcaaaa agcagctttc
     8041 ggtctatagc gccgttattg gcgtacattt ttttcttctg ggcacctatc ccgctggtgc
     8101 cagtgccatc cttaatgcca ttcgtacatt gattacctta cgcacgcgca gcttatgggt
     8161 aatggcgatt tttattgtgc tgactggcgg aattggcctc gcgaagttcc atcatcctgt
     8221 cgaactattg ccggttatcg gtacgattgt cagtacctgg gcgctgttcc gctgtaaagg
     8281 gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc tgggtgattc acaacttctg
     8341 ggcggggtcg ataggcggca cgatgattga gggtagtttt ctgcttatga atggcctgaa
     8401 tatcattcgt ttctggcgga tgcagaaaag gggaattgat ccgtttaaag tagagaaaac
     8461 ccctcccgcc atagacgaaa ggggttaaac aattaattac gcagggcgct atttgccaga
     8521 cgatcgtctt ccgcctggca agctgccgca gtgaacagca cgtcagttga agagttcagc
     8581 gcggtttcgc aagagtcctg caatacgccg ataataaagc cgacggcaac cacctgcatg
     8641 gcgatatcgt tcgaaatacc gaacatatta caggccagtg ggatcagcag caaagaaccc
     8701 cccgccacgc cggatgcgcc acaggcacac agagaagcca ctacgctcaa cagcagcgct
     8761 gtgggcagat cgaccggaat acccagcgta ttaaccgcag ccagcgtcaa cacggtaatg
     8821 gtgattgctg cgcccgccat attgatggtg gctcccagcg gaatagaaac tgaataggta
     8881 tcgcgatcca gattcagctt ttcacacagc gccatattca ccggaatgtt cgctgcagag
     8941 ctgcgggtga agaaggcata cacaccgctt tcgcgcaggc acagcagcac cagcgggaac
     9001 gggttacgac gaattttcca ccacaccagc aatgggttaa ccaccagcgc caccagtaac
     9061 atacagccaa ccagcacgac cagcagttgc gcgtagcccc acagtgtgga gaaaccggtg
     9121 gttgccaggg tagaagaaac cagcccaaaa ataccgaacg gtgcgaagcg aatgaccagt
     9181 ttcaccataa aggtaacggc attcgacata tcattgacca ggtttttggt ggtctcgtta
     9241 ccgtgacgca gtgcgaagcc gaggccgatc gcccacacca gaatcccgat gtagttacct
     9301 ttcagcaggg cgtcgatggg gttggaaacc atgctcatta ccagtccgcg catcacttcg
     9361 acaatgcctg acggcggcga aatatcaccc gcgctactgg acaagtgcag ggtagaaggg
     9421 aaggcaaagc tgaagactac tgcggccaga gctgcagaga aggtgcccag cagatagagg
     9481 aacaaaatag gacggatatt ggttttctgc ccgtgctggt ggttagcaat agatgccatt
     9541 accagcatca acaccaggat gggggcaaca gctttcagtg cgccgacgaa caaagtacct
     9601 aacagaccaa cagcttccgc cgcgggtttt gagatccatg ccagaagaat ccccagaaca
     9661 aggccgacca ggatttgttt taccaggctg ccatgagcca gacgccggaa tagccccggt
     9721 gaacgttgcg tagtcatttt tcgatccttt cattgtgttg tcgcacatcc ctggtgcgtt
     9781 ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa ggtcgagtat aaggaaagtt
     9841 acagcttcgg gaagcgaaaa atgctggatt ttaaagagag gtcatgtttt gcaacttaat
     9901 ggttgcattt atttaacata aacagaggga aggactggct actaaggctg attagataac
     9961 atatgggcaa aactatttgg aatatgagaa cggttgcgct cctttaacgt gttatgccat
    10021 ataaagtcac tggcgcaggg agcgcgcagg gggcggccaa tcgccgccgc cccctgcacc
    10081 cccaggctct ggcgaacaaa atcgccgctg cgcggttccc tcagcctatc ccttgcggct
    10141 accgggtcgg gcgcaaggta acgtccctgt aagatgcgcc ctcgccccgg cctccaggga
    10201 acgcttcggc gattttgacg ccaccaacac ccgtgcggtc tttttgtaaa gagctataag
    10261 ccgattacat aattaatagc tcacctttgt ctggaatgag ttatctctag gaatggctta
    10321 atatacagcg aatcattctc cccgctgctt atcatgccga taattcaccc aggcgttgat
    10381 aataaacgtc atcaccagaa tgccaaacac cacgcccagc gagacggcga ttggaatatg
    10441 gtagaagtcg acaatcagca tcttgatacc gataaacacc agaatcaccg ccaggccata
    10501 tttgagcatc gagaaacgct ctgctacgcc cgccagcagg aaatacatcg cacgcaggcc
    10561 gaggattgca aacaggtttg aggtcagcac aatgaacggg tcagtggtca cggcgaagat
    10621 agccggaatg ctatccacgg caaaaatcac gtcgctcaat tccaccagaa tcagtaccag
    10681 catcagcggt gtggcgtaca gcaatccatt cttacgcaca aagaaatgct cgttgtcgat
    10741 ggtgtcggtc atgcgcaaat gaccgcgtag ccagcgcacc agcggcttgt cgccaatgcc
    10801 tgattcatct tcatgggcga gcgccatctt cacgccggta aacagcagga aggcaccgaa
    10861 gatatacagg atccagtcga actgcgaaat cagccagctg cctgtgaaga tcatgatggt
    10921 acgcagaaca atcgccccga gcacgccata caccagcacg cgacgttgta atgccgccgg
    10981 aacggagaaa tagctgaaca acatcagcca gacaaagacg ttatcgaccg ccagcgattt
    11041 ctcaatcaga taacccgtga gaaaggccag ggcctgtgga tcggcaacgg cgcgaccttc
    11101 ggtttgcacc agataccacc agaaagcggc gttaaacagt aacgacagcg tcacccagac
    11161 cagcgaccag gccgcagcct gtttcatggt catggcatgt gccccacgac gcccctgcaa
    11221 caacaggtcg atagccagca taatggcgac aacaacagcg aatccgcccc atagcaacgg
    11281 cgtgccgaca gtattcatag aagttcctta cacataaaaa aacggccaac gtcggaagac
    11341 gtcagccgct gctttttatg catagacctc gccttccggc aaggtctcac ttacaacaaa
    11401 aaaggaatac gtctgcgtat tcctttcggg ttgcccggtg accggatgtg gtttttcact
    11461 catcgtaatg acgatcgacc ggcaatgaag ttactcccct ttgcaggtaa caaagtatga
    11521 cagaccattc agtcagtcaa tggtctgtta catacctttt acactgtttt actttggcaa
    11581 gtatagtttt acgctatctg ccggaaaaat caccccagtt tggcggcgga tctcggtcag
    11641 cagtttggct gtgatgcgac tgaccgccag ccctggatga tccaccagat gctcatccac
    11701 cagggtagcg aacagctctg cttcgtagag catggtatta atatgctgcg gctgggtgag
    11761 atcctgcatc tggctgccac gcggcacaaa gcagactttc tggcattcag acagtttttc
    11821 aatcaccagc gatcccgctt cgccctgaat ctcgcttccc aggacagaat cactgacctt
    11881 tgagtgctgc aaggtgacgc tgaaatcacc gtaatccatc accaccacgc cgtgggcgtc
    11941 aacgccgctt gccagcaaac tcgccgttgc ctgcacgctt ttcggctcac caaataacgc
    12001 caccgccgac gccaggcagt aaaagccgat atccataatt gaaccgttag agaatgccgg
    12061 attaaaggtg ttgggattct caccgtccag gtaacgttga taccgcgagg aatactggca
    12121 atagttgaaa aagactttac gcagtttgcc tgctttcggc agcgcctggc gcaacaaatg
    12181 aaagttcggc aggcaggcgg ttttaaatgc ctcaaacagc accacctgat tttcgcgcgc
    12241 acaggcaatg gcggcatcca cttccgccag attcgacgcc agcggttttt cgcaaatcac
    12301 atgaatttta tggctaagga aaagttgtgt ctgggaaaag tgcagggaat tcgggctggc
    12361 aatatacacc gcgtcaatgg catcgctttc cgccatcgct tccagcgacg taaacagatg
    12421 ctcgacagaa aaatcattgg cgaagtgctg agcctgctca aggctgcggg aatatacggc
    12481 ggttaacttg tatttaccgc tctcatgggc ggcctcgacg aactggcgag tgatccagtt
    12541 cgtaccaatc acagcgaaac gtatcataac gcgtggatac tccataaagg gattctgtcg
    12601 tcaatgtagc acgccttttt catcttcgac gtgcgcagat acgcattatt ctttcgccgg
    12661 acagccatgc tcactaaacg gcccaatcat gaccgatggc ccgagatatt tcggctggcg
    12721 atggaggatt tttgcgtcaa tcaccgcgct gacacgagcg agtttctccc gcagttgagt
    12781 ataaaaaccg tgcttcatat tgaggtctgg cgcgttaaac ccgatagcgt cgatgccgta
    12841 ctgttttgcc agccagatgg tgcgttggtt atgaaattct tgcgagataa tggtgatatg
    12901 gttttcccca aacacctttt tggcacgcac taccgaatcc agcgttgaga atccggcata
    12961 gtcgcaaaag atcactttcg ccggtacgcc tttagcgatc aaagcctgct gcatacctga
    13021 tgcttcatcg taatttttac gtccgttatc gccactcacc agtagccatt tcacttttcc
    13081 ggcgtggtac aacgctgctg cggtatcaat acgtcgggta aagtagcgat tacccggcct
    13141 cgcccccagc aataaaccca cgttacgcgc cgggacggcg ttgacatcgc cccaggtcag
    13201 ctgcttgctg gcgttgacca taacgcgatc ggcgataaaa atggtcgctc ctgccacaag
    13261 taacagtaag caggcaatct ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg
    13321 ggcaaatgcg cgcagcatgg tcgttatcct taacgaaagg ggggaggtcg attttaggcg
    13381 ctaaagaaag aaaagataac gcctgacatc attttcagat tttgtcctgc ccaagcgaca
    13441 actgcgttag ccacagccgg gtgtcgaact caagctggtg gtactgcggc tccatatggc
    13501 agcacaattg atagaacgct ttgttatgct ctttctcctt caggtgcgcc agctcgtgca
    13561 ccacgatcat gcgcaaaaac ggttccggcg cgttgcgaaa cacggtagcg acgcggatct
    13621 ctgcttttgc tttcagcttg ctgccctgca cacgagaaac agcggtatgt aacccgagcg
    13681 cgttttttag cacatggatc ttattgtcat acatcacttt attgatcggc ggggcattac
    13741 gcagaaactg atttttcaga tcctgagtat attgccagag agctttatcg gtggcgtagt
    13801 cgtgtgttcc cggatagcgt tttgccagca catcgcccag acgctgttcg ttaatcagtg
    13861 ttcgcacctg cgaaagcagc tgctcgggat aaccctgaag ataagtaaga ttgctcataa
    13921 ccgcccacga aaaagagaaa aagggtatac tcacgcaccc ttttcagggg caacgtcgaa
    13981 attttatcat tcaggagggc cgatgagcca cttagacaac ggtttccgtt cactgacact
    14041 acaacgtttt ccggcgacgg atgacgttaa cccgctacag gcgtgggaag cggcagatga
    14101 atatttgctg caacagttgg acgacacaga aatcctcggc ccggtgttga tcctgaatga
    14161 tgcctttggt gcgttaagct gcgcgctggc ggaacataag ccgtacagca ttggcgactc
    14221 atacatcagt gaactggcga cgcgcgagaa tttacgccta aacgggattg atgagtcgag
    14281 cgtgaagttt ctcgacagca ccgccgacta cccgcaacag ccgggtgtgg tgctgatcaa
    14341 agtgccgaaa acactggcat tgctggaaca gcaactgcgt gcgctgcgca aagtggtcac
    14401 gtcggataca cgtattattg ccggtgccaa agcccgtgac attcacactt ccacgctgga
    14461 actgttcgaa aaagtgctcg gcccgaccac caccacgctg gcatggaaaa aagcgcgcct
    14521 gattaattgc acgttcaatg aaccgccgct ggccgatgcg ccgcagaccg ttagctggaa
    14581 gctggaaggt actgactgga ctatccataa ccatgcgaat gtcttctccc gcaccgggct
    14641 tgatatcggc gcgcgcttct ttatgcaaca tctgccagag aatctcgaag gggagattgt
    14701 cgatctcggt tgcggtaatg gcgttattgg tctgacgctg cttgataaaa acccgcaggc
    14761 gaaagtggtg tttgtcgatg aatcgccgat ggcggttgct tccagccgtt tgaacgttga
    14821 aaccaacatg ccagaggcgt tggatcgcag cgagtttatg atcaacaacg cgctctccgg
    14881 cgtggagcct ttccgcttta atgctgtgct ctgcaaccct ccgtttcacc aacaacatgc
    14941 gctgaccgat aacgtcgcct gggagatgtt ccactacgcc cgccgctgcc tgaaaatcaa
    15001 cggcgagctg tatatcgttg ccaaccgcca tctggattac ttccataaac tgaagaaaat
    15061 tttcggcaac tgcaccacta ttgcgacgaa taataaattt gtggtgctga aagcggtgaa
    15121 gctgggacgt cgtcggtaag attgggagtt acctgccgga tgcggcgtaa acgccttatc
    15181 cggcctacgt tcagcacgtt acccccaggc cggataagac gcggcaagcg tcgcatccgg
    15241 caatgtctgc tcagttctgg aatcgcgctt ccataaaaca aaaaatccct gtcaccatcc
    15301 tctacattct cttgtttagc gtttttctac gtttattctt ccgtcacaca gataaattca
    15361 tccgttgcca atctgtcaac ggatgttatc atattgccaa tttgtcaacg gacgtgatga
    15421 agaatgcacc tgataactca aaaagcattg aaagatgctg cggaaaaata cccgcaacat
    15481 aaaacggagt tggtggctct ggggaacacg attgctaagg gatatttcaa aaaacctgag
    15541 tcattaaaag cagtattccc atctctggat aacttcaaat atctggataa gcattatgtt
    15601 ttcaatgttg ggggcaatga attacgtgtt gtagcaatgg tcttttttga atcgcaaaag
    15661 tgctacatac gtgaagttat gacgcataaa gaatacgatt tctttaccgc tgttcatcgt
    15721 actaagggga aaaaatgatt gcgattgccg acatcttgca agcaggagaa aagctaactg
    15781 ctgtggcacc ttttctggcg ggtattcaga acgaggaaca atacacccag gcgctggaac
    15841 tggtagatca tctgctgctc aacgatcctg aaaacccctt gctggatctg gtgtgtgcca
    15901 aaataaccgc gtgggaagaa tcagcgcccg aatttgcgga atttaatgcc atggctcaag
    15961 ccatgcctgg cggtatagcc gtgattcgta cccttatgga tcaatatggt ttaacccttt
    16021 ccgatctgcc ggaaattggc agtaaatcta tggtgtcacg cgttttgagc gggaagagga
    16081 aattaacgct ggaacacgct aaaaaattgg caacgcgatt cggcatttct cccgccttgt
    16141 ttattgatta acgcgacgtg ccgaatgcgt tgcatccggc gcttcaaata acgatttaaa
    16201 tctccagcgc cagccgtgtt ccctgggcaa ttgcccgtcg tgcgtccagc tccatagcca
    16261 catcgcagcc gccaattaaa tgcacagttt tcccgctatc aatcagcggt tgcgccagcg
    16321 cgcggtttgg ctcttgccct gcgcagatca ccacattgtc cactgctaat acctgcgttt
    16381 cgccgttgat caccacatgc agcccgtcat cgtcaatctt ctgataactt acgcctggga
    16441 tcattttcac accccgcgag agcagggtgg tgcgatggat ccagccggtg gttttgccta
    16501 acccctgtcc cggtttgctg gctttgcgct ggagcatcac aatttgccgt gggctacgcg
    16561 ggatctgcat tccctgcggg cttaagccac cagcctgttg taggctgctg tcgatccccc
    16621 actcattaca gaacctggcg atattctggc tggtggattc gcccggctga cttaaataca
    16681 tcgccgtatc aaaaccaatc ccgccacaac cgatgatggc aaccttgttg cctaccggcg
    16741 ctttgtcgcg cagtacatcg agataactca ataccttcgg atgatcgatc ccgtcgatag
    16801 gcggaacacg cggcacgatc ccactggcga ggatcgtttc atcgaacgcc tgtaactgat
    16861 ccgccgtcac ggtgtgattg agtttcagcg tcacgcccgt cacttcgatc atccggcggt
    16921 agtagcgcag cgtttcgtaa aactcctctt tgccggggat ctgtttggcg atattaaact
    16981 gcccgccgat ctcgctgtgc gcatcaaaca atgttacctg atgcccacgc gccgccgcgt
    17041 taatggcaaa cgccagccca gcaggtccag cgccgaccac cgccagattt tttttctgca
    17101 cggcgggaag gattggcatt ttggtttcgt ggcaggcgcg aggattcacc aggcacgagg
    17161 tgactttgcc aacgaagatc tgatcgaggc agccctggtt gcagccaata caagtgttga
    17221 tctcatcggc tcgccctgat tgcgcttttg acagcagttc cgcatccgcg agaaacggtc
    17281 gcgccatcga tactatatcg gcatcgccgc gcgagagaat atcgtcggca acctgcggat
    17341 cgttaatccg gttggtggtt accagcggca gcgagacgtg gcctttcagt ttgcgcgtga
    17401 cccagctaaa tgcgccgcgc ggcacgggcg tggcgatggt cggaatccgc gcttcgtgcc
    17461 agcctattcc ggtgttgata atggtcgcgc ccgccgcttc aatggcctgc gccagttcta
    17521 ccgtttcggc aaaagtgccg ccgccttcga ctaggtcgag catcgacaac cggtaaataa
    17581 taatgaagtc gttgccgacg cgttcgcgca ccgtacgcac tacttctacg gcaaatcgca
    17641 tccggttgcg gtaatcgccg ccccactggt cactacgctg attggtgcgc agcgtcagaa
    17701 attcgttgat caaataccct tcagagccca tcacttcgac gccatcgtat ccggcctccc
    17761 gcgccagttg cgcgcagcgg gcgaaatcgt cgatcagttg caggatctct tcatgggtga
    17821 gttcgtgagg aacgaaacgg ttgatggggg cctgcaatgc ggacggtgcg accagatgcg
    17881 gttggtagct gtagcgcccg gtatgcaaaa tttgcagggc gattttgccg ccttcctgat
    17941 gtacggcttc ggtaatggtg cgatggtgtg ggatctggct ggcgtcgttg agcattgcgc
    18001 caccttccat gccaacgcct gttaaatctg gtgcaatgcc gccgctgaca atcagcgcca
    18061 cgccgtgacg ggcgcgttcg gcataaaacg cagccagccg ttcagcaccg tccgggtatt
    18121 cctccagccc ggtgtgcatt gaacccatca acacgcggtt ttttaacgtg gtaaaaccta
    18181 aatccagcgg ggcgaacagc gacgggtagc tcataaaatt gtccagtatg taaaataatt
    18241 gttatgtagt cggatgagtt ctaatttagc cgtcgatgga gaaaagggaa aaggggagtg
    18301 cggggtttgt gatgggattc aaaaagaata cgcccggtgg taacgccgga tgcgacgctt
    18361 gccgtgtttt atccggcttg ggggattgca catgtagggc ggataaggcg ttcacgccgc
    18421 atccgccagc cgttgcaaat tactgcttac ggaaaaaatc gttatacaac atatacaaat
    18481 gagcggcact ccaggagaaa tttggtgcgc cttgctgcgc gcctgtcagc gggttgtaat
    18541 tctcctgaat tgggccatcg gcggttaatc ctttggcgtg cctgaagaac gtatccgcca
    18601 gcttcagggc atcatcacga taaccgtaac gctccatccc tttcagacca aaccagaact
    18661 gatccaccca tacgcgcccg cgccagtaga tatcagcgcc aaaagccgga ttggttaacg
    18721 ccgccgttcc cagcggaaca aatgtgttga actctttagg atcgagcatc accttcacca
    18781 ccgcgtcggc attggcctgc gttgccgcac cgttaaacag cggcgaccag ccttccggcc
    18841 ctttaccgcg ctcaacaatc ggtttgcccg cgcagccgtt cgccagtggt ttatcttcaa
    18901 tacgtacgtc atagtagaac tgggtagtcg ggtcgaacat acaggtgttg atgtagtccg
    18961 cgagctgctg tgccaactgg cgatagcgtt tggcctcttc tggcttaccg aggatggttg
    19021 ccatctccgc cagataatgg ttatcgctgt acatatagct ggcctgatcc accgactcct
    19081 gcaatagcga gtagcccagc aacgttccgt cctgactgcg gttttcggcg aatttcaccg
    19141 tccagtcgct acgtttgccg ccattagcga catatttatc caactgttct ttgtcgataa
    19201 aaccaaagac ggcggcatca tcacgccccg attcccacga cgcagcgacc tgtgccggaa
    19261 tttccagact gtcgtactgg cctttctcca ccacgcgggc gtagttgttc agcccagact
    19321 gcgtctcttc tttatcgcct tttttcaccg taaacagcat ctcgccgctc tcggtgttgt
    19381 gggctttgtc gcgggtcgcg ccatattccg gcaccccgtt gccgttatga tcgcggttac
    19441 gtaaccacca gtcgtgatag gccaccagtt tcgggtacat ctctgccaac caggctttat
    19501 cctgggtgac gttgtatact tccatcaccg accaggcggc aaggctgggt ttggtattac
    19561 gttcgttcca gttgccgcca tcgccaccac gctcggggct aaggttccac gctatcaggt
    19621 cggggacaaa gcccacatcc tgcggacgca cgctgtcgcc aggctggatc tgccaggaga
    19681 agaccgcgcg gatattctct ttggcgatat ccggattgaa atgcgccatc gcaaacgcct
    19741 gcttccaggt atcccacggc caggtctgat tgccggagaa ccagcgtccg gtcaccgacg
    19801 gcgtgacggt gttataattc accgcaccgc ccggtgagcg ccagttaccg ttgagcgttt
    19861 cgatcgcttt caccgcgacg cgcgtctgtt ccggcgtcgc atccggattg gttaaccctt
    19921 tcttcaggta ttcttcccag cgttgctgcg aggcggtgag ataaaacgcc ggacgcgcca
    19981 gaatatcgcg gatctgcatt tgctctttgc taacttcctg agcggtcagc agatgggaat
    20041 aggtggtgta gagcgtggtc gaaccgttga tatgcgcctt actggtaaag cgattaccgt
    20101 tgatttcagt ctgcaccggc agggatttat gcacctgata ttccgattcg ccggaggtca
    20161 gcagatccca ggtggcgcgc actttgccaa aggtgacttt caggccatca cgggtggcgc
    20221 tgattttgcg ctgatagtca gggtattcgc cagcaatggt tttatcggaa agcggtttcc
    20281 cttctttcgc ttccagtttt tccagcagtt cgccatccca caccagatcc agcggtttat
    20341 tgctgatgat tttagtttcc agtagtgacg tgcgcggcgt ggcgaagcgc agagtcattt
    20401 cgacctgcac atcttttgct gtcagttttt gcaccagcgc gccgggaata ctgtatgcct
    20461 ccagcgtgaa gttgactttc ttgccgtctt gccagacagt caggcggtcg aaattgctgg
    20521 ccataaagtt gatgtattct tccgtcagca gcgcaacgcc cggaaagccg cccatggtgt
    20581 tcgggccgtc tggcaacaga tgaccatgcc aggctccgag atcgaaaaac ggattaaaac
    20641 gctggtgatc gtcgtaatcg taatccttca tgtactgcgg cgcaccagta cggttaatca
    20701 cgtttttata attgtcggcg ttagcggcat gagcggaaaa acttatcagc agagcgcagg
    20761 ttactggcgt taaaatagtt ttgattttca tgagattaca gtccttatta ccagtagaaa
    20821 tattgcatca ggaagatttg gtcgctggga tcgttagagg catttttcat gtagaagcgg
    20881 ctatcaaaag ccgtggcgaa acgtccgccg ccatattcgt accagatacc aaactgcatg
    20941 aaagaggtgg tttcatcgtc agcgtggtca gggcgatgtt tggcgtaggt gtaagcggtg
    21001 gagagataaa cgccttttaa cgcttcatac atggcgctca ccatcatccc gctttcactt
    21061 cccggtacgt ccagcccgtc ggcacgtccg gtatggtgcc aggcgcgagc ggcaaagtta
    21121 ggggtaaaca ggcccgtgag atagagttgt cctgtgccgt cggtaatttc cagaccattc
    21181 atccaggtga tgccttcggc aaggtcatac tggatgtaac cgttcatcca ttgccggata
    21241 ggtgtatttg tcgtcgtagc gatcgtactt gccaaaatgt agccaggctt tgctttcgtc
    21301 atgatgtccg gcaggtgtgg cggtgacgct ataacgaaag ttgccgctta agttctgtac
    21361 cttcagcgcg gtcataatgt cgcgggtatt gggaatgacg tagcccaaat cgggtgtgaa
    21421 gtcgccccac cattgcaggt catccagcga ggaatcctga cgcagactga gcatgatttc
    21481 ggtgccatca ttggtacgat aaccgccata gaaacggtta atcccacctt cgaaaccacc
    21541 ccagctatgg tcaggcacct aggcattacc gtcgtggtcg gcctgcacgg tccagccttc
    21601 gccgtaaatc aggccgaacc aggagccgta cttcatctcc aggccacctt caatataggt
    21661 gccgtcgctg tatttatgtt tattctcacc ctctaaatcc atatatccac caagccctaa
    21721 ttcgccgtaa aaacgaaatg aaggagaaga tgtcgaggta attgcgggtg tttttgtgtc
    21781 gtcatgttct gccggacgtg cttcttctcc tgccgcagaa aaggcgtagc aaaacggcag
    21841 agcaagtaat gctctgcatg gtgcagtgat taatttcatt gtgttaatcc atgagctaaa
    21901 gaaaaagtac agccttttat cgttgtaatt ataaaaatat aaaggcagaa actattttcc
    21961 ctctcctcat ccaggaagag ggaaaatgcc tgctcatttt ttattagtct gcttaatcaa
    22021 cattgctggc tggcgtcgct tcaatatggc gctcttcagc cgttaatccc tttatatatt
    22081 tgctgtattt ccaccacgca aagccgagga agataacaat accgccgacg ttataaaaaa
    22141 tgatggtcag aatattcgcg ccagtcggga atgtcgaagc gacaaaccct acggcaaata
    22201 tcgcaatcag cattgaaaca acgataatac cggtgcggcg ggagcccata cggaaatcgc
    22261 gtggcaggtg atctaatttg gcgcgtaaat tcaggtaagc cagcatgatg aataacggcg
    22321 gaagcatgga cgctgcggcg gtcatattaa taatggtatt catcagatcc tgcacggtat
    22381 tggaaccgag catcgggata atcatcagcg ggatgacgat cagaaactgg atccatgctg
    22441 cgcgcgccgg aacgccgttt tcgttaagtt cgacggtttt cttaccaaag atgccttccg
    22501 ggatttcgga gaagaaaatt ttcaccggcg ttgcggtcca catcagcagg gaaccgaaca
    22561 ttgcggtaaa ggacaccaga ccgacaaagc gattcatcaa cgcttccggc agaccaaaat
    22621 acgccgccat gccgtggaat acctgcaccg atccgccggt aaatttcaac tctttgctgc
    22681 tgacgaagac gttaatcagc accgaggaga cggaatacag tacgccgata aaaatcccgg
    22741 cgaggatgat cactttaacg aacgatttcg aaccgccttt gacgtcgtta acgtacaccg
    22801 cgacggactc cgcgccacct gcggccataa agatccaggt ggtaacgccg aggaacgccc
    22861 agttgaagtt cgggatcatc gcgtcaacgg tgatcggatc ggcaggctgt acgccgccaa
    22921 ccagtgccgt acctgccagt aaaatgtagg agagcgttaa cagcagcatc agcgttgaag
    22981 tgacggaggt aattggcccc agcattttcg ccccgttggt ggaaacccag gtggagaagg
    23041 cgaacagcac catactgata acggtggtgg caaccggcgt cataatatat tcgtagccga
    23101 ggaaggcata cgaagcataa gcaataacgc gcggcaacag cgaggtgaaa aagaacagat
    23161 ttacgaacca gtaagtgtag gcagtaataa atgcccaacg tccgcccagc gaacttttta
    23221 cccacgcgta gacaccggct tctgagtttt tatttaacga aacaaattct gcgatgatca
    23281 gacagaaggg aataaaataa aaaatcgtcg cgaggaaaaa catcggtgcc gaggccagtc
    23341 caagctcaat attattattg ataacgttgt taaagctgta aacggcggca aaagtcagcg
    23401 agagcaagcc gaatttgccg attgtattac gtttggtatc agacataaca catctccgaa
    23461 aatgtgtagg gtagcggtgg cggcaaactc cttttgccgc cgtagttgtt atttgttatg
    23521 gaaataacca tcttcgatgg tgactttgag aaccactttt ttgaccgcgt tattgcagat
    23581 aaaccgatac gcttcatgga tatcgcaaat aacgatttgc ccttcgtgga cctctacggt
    23641 ttctccgcag ccttttaaat attcacggtc agtttcatca cgataatatt ccactacctg
    23701 taatgtctct ttcggcgcat attcaatttt ttgctgccct tgcaggtagt aatgcacttc
    23761 aaaatagcgt cgatgcccgg taaatagcgc atcggtcgcg gagtcatttt ccacacggta
    23821 ggtcaatgaa tcaccgatgg agtgggcgac gccaggctga atgttgtcga tattttcaat
    23881 cgcttcaacg cagcgttgcc acttcttgcc agaggcgtaa atctggcgga actgttctaa
    23941 gttatcgatg atcctcattg cttattctcg ctgtgcaaat tcgtggaaaa gaaccctgcg
    24001 ccgaactcat acgacgccag gctttgcgcg gtagcttctc cgccagaaac cggcagcaac
    24061 gtaaagccgt agctgaagtc acggaaccag acgcgccagg agtccagcac ctcgctgccc
    24121 caggagttgg agccgaggcc aagtagctgg tggtcgaggt tcagggtgat gtcatcactg
    24181 cgctgcagct cgttacagtg ctgggcagcg tggatgtttt cctgggtata gtgccaggcg
    24241 ctgaagttaa ttgggcgctg cggaaccacc agcagaccgt tgccgtggcg gttagtcagt
    24301 gccgtccagc ggacatgctg acggttgccg ttgttctgcg ggaagggata gttctcgaac
    24361 atggcatcga cggtgctgcg ccagatatcg atgatgttag cctgctggct gtcggcgtag
    24421 ttttcgcccg gtccacgacc gtaatacgcc acctgatcgt attcgccgtt aatccccatg
    24481 gtgaaaccga tgcacggaat gatgtgcgga tagtcgccgt aacgctcgcc ggaaagcgcc
    24541 acgttaacct ggccatcggc agcgatgcgc cagatgtagg tacagcgcat cccgaagtca
    24601 aacaccggcg gtgcaataac tgtgcggctg atgatcaaca cttcaccatc gctctgttct
    24661 acggcaaagt cgcgcagatg ttcctgcatg atctgcaaat gattcggctg ccacaaccct
    24721 tcgtactcct gcttgtggtt gtcgatcatc ggcttgaaga agttgatctt tggctcgcgg
    24781 gtcagcagcg actcgccatt tacctgccag gatgtcggtt tgccactcat ttttgagaag
    24841 gtgatcgcga agttgtagcc gcgaacggtg cagctcaaac gatcgtcttc cagcgtcagc
    24901 ggacgcgcat tatttggtgc gaaaggcact ggctgcgcgg tgttttcctt cagcgggaac
    24961 tgataagtgg cgatagaatg cccggcttcg ctgtagcggg tgcgggaatc tttggtcacc
    25021 gtaatgttga ggaacgcttc gcgggcgtcc agctgcggca gcgtgatctg caagggggct
    25081 tcgctgttcg gcgcaacgtc gcgcagttta atctgctgcg tcgcgagcgt ttcaccttcg
    25141 gcgcgcacct ctgcgtgcag ggtgtagtca tcaagcgtgg taaaccacag tttattttcg
    25201 actttcaact cgccgcgagt cagcgcgtgg atttttaccg gcgcgataac ctgtttgtac
    25261 tctttcaggc ccgatcccgg cgtctgatcg gaatagatca aaccatcaag acagaagtta
    25321 tagttgttgg gatagtcgcc gtagtcgccg ccgaatttat accagacatt gccgtggtcg
    25381 tcctgtgcct ggatcccgtg gtcgcaccac tcccagacat aatgaccctg aatgcaatcg
    25441 tgcttataga agacgttctg gtactccgtc agcccgcccg gtccgttccc catcgcatga
    25501 gcatattcac agatgatgcg cggcttcgga tgcgggtatt caccaaactc attcatcagc
    25561 ggcacgcggg tgtacatggt ggaaataata tcgaccactt cagcatcgcg atcttcttcg
    25621 taatgaacca gtcgcgtgtc atccagcgct ttcgccgcat ggtacatcgc gcggatgtta
    25681 cagccatagc cggattcatt gcccagcgac cagatgatga tcgacggatg gtttttctgt
    25741 gcgtggatat ggcgaacaat gcgctcgacg tagacttttt cccactgcgg atcgtcggta
    25801 atacggctga tatcgccgac attagcaaag ccgtgcgatt cgacgtcggt ttccgccatc
    25861 acaaacaggc cgtagatatc acacagttcg taaaaacgcg gatcgttcgg gtagtgagcg
    25921 gtacgcacgg agttgatatt gtgctgcttc atcaactgga gatctttctc gacgcgatcc
    25981 attccaacgg cgcggccttt gcgatgatcg ttgtcgtgac ggttgacgcc gtgcagcatc
    26041 acataacggt tattgatcca gaacagaccg tcgcgcactt tgatatcacg gaagccaacg
    26101 cgttgtggca ccacttccag aacgttgccg ttggcgtctt tcagcgtcat gaccagatgg
    26161 taaagataag gggattctgc tgaccattgc tgcggctgtt cgacagtaaa agcaaagctg
    26221 gcgctggtca gtttttcaat tgccaaatga tcaatggcgc tgctgtgcac cacgcgttcg
    26281 ccatcaaaca gggtatattc cagcgtcgtg acgacagggg aggcggcgag attttccagc
    26341 accacttcgc aggaaagcgt ggcatcgcaa taggcttcgt caaagtcggt acgcacagtg
    26401 aaatcgttaa tatgcgttag gtgttttccg accagataaa catcgcggaa gatccccgcc
    26461 gaccaccaca tatcctggtc ttccacgtag gtagagtccg cccactgcat cacgcgcaca
    26521 cacaacaggt tgtcgccggt tttaaccatc gcgctgatgt caaactctgc ggtcaggcga
    26581 ctgcccttgc tgaaacccac atactgaccg ttaacgtaga cttcaaaata ggtttcgacg
    26641 ccgtcaaatt taatcagcgt ctgtttaccc tgccagccgt cgctgagggt gaaaatacgt
    26701 tgataggcac cggttgggtt atcgctgggg acaaacggca catcgatggg gaacggaaaa
    26761 ccttcgtcgg tatattgcag tttgccgtga ccttccattt gccacatggc ggggacggta
    26821 atatgccccc agtcagccat taactcagag gtgaaggctt ctggtacttg cagcggatgg
    26881 tcaaaaaagt ggaaattcca ctgactgctt aagggcagaa acaggctgct ggtttcgcgg
    26941 gcaaaggtac gcgcttgcgc aacagaatca tatgaaaaaa agtacgcacg cggcgcaagt
    27001 cggttttcgt gggtgagctg aatgttttcc cagcgattca tagggcctcc cggtagagag
    27061 aaatcggaac cattcatggt gatggtagtt tgtgtaattg ctgggggttt agtaaaattt
    27121 ttactaaaaa gcagcaggta aatgcattta ctttaactgg atcacgaaaa taaacggcag
    27181 cactgtccgg caaaaatgat tgccgaacag tgtgtaaggg gatttaacgg gtcgtgccgc
    27241 gcagttttaa tttgctggga acgaagacta gcagcggcag cgcgcgacca tcgcgggctt
    27301 tttcatacac caggttaacg ccctgacttc ccatcatctc ggaatggatg cgcacggtgg
    27361 agagcggcgg aaaggtaaat cgcgcggtgg ggatatcgtt aacgctgata agcgaaatat
    27421 cttgtgggat gttcaggcca cgttcatgaa ttgcccgcag tacgccgata gcaatggaat
    27481 cggaagcaac aaacagtgcc ttcggatagt cttcccgcgc cagcatttgt tttgccagtt
    27541 cataacccga cgaactggaa aaaccgccgc gccagatatc ctcttcgcgt accacttgtt
    27601 tcagtcggcc atattccgca aaggcgacct cacgaatatc cgccttgcca ggctcatctt
    27661 caccgccaat aaaaccaata cgattaacgc cctggttgat atagaagtcg atgatttctt
    27721 tactgatgcg tgccagatcg atatccaccg catcgtaacc gctgccgggt tcgtgaaagt
    27781 cgataaaaca gatattgtcg gtcaacgcgc tggcagcggc gcgcagggcg ggcgtgggtt
    27841 tgccgacaat taaaataccg gtgacgtttt taatgtctgg taatccgttg tgttcataac
    27901 agttggtcag ttcgatcgcc agcttttcgc actgagtttc aatgccgtgg cggatcgcca
    27961 gatagtaagg atcgttgatc tccagctcct gctggtagct gtagatagcc agaatatggt
    28021 gttggttgac tgcgcctgtc tgtagtttac gggcgctact ggtcttgtac tccagctttt
    28081 cggcgatctc gagaatgcga tgtttcgtct cttctttcac attcaatgtc ggatcgtcat
    28141 ttaagaccct ggatactgtc gccagggata cgccagcttc gattgcgatg tcttttaatg
    28201 ttgccatgtt tacctttttt actttccctg atgtgcgccc ccattgtaga ggaagtgctt
    28261 atcgggtcac ctgttttcag taaataaagc gcgtttcttt gacgctgtaa cggtaacaca
    28321 gcggcttagg aatgtgatca cgcctgcatt ctccattttt agcctacagg attgagaaaa
    28381 acttttctat agtttagtaa aattttactc gaagggagga agcaatggaa accgtggctt
    28441 acgctgattt tgcacgtctg gaaatgcgcg tcggaaagat tgtggaagtg aaacgccatg
    28501 aaaacgccga caagctgtac atcgtacagg ttgatgtggg gaaaaaacac tgcaaaccgt
    28561 gaccagcctg gtgccgtact acagcgaaga ggagctgatg gggaaaacgg tggtggtctt
    28621 atgcaatctg caaaaagcga aaatgcgtgg tgaaacgtcg gaatgcatgc tgttgtgtgc
    28681 ggaaaccgat gacggcagtg aaagcgtgtt gttaacgccg gagcggatga tgcctgcggg
    28741 cgtacgcgtc gtataaaaag atcggatggc gacgtcgtat cgccatccga tttgatatta
    28801 cgcttcttcg acacttactc gcatggccgc cagcgcctta cgcgccgctt tgatcaccag
    28861 ttcacactgt tcaatggtca gtgtcagtgg cggttcaatg cggatcgttt tggcgttatt
    28921 gagcgttccg gccaccagta cgcgctggcg gaacatctcg ctggcaaagt tatagccgat
    28981 ttcgttatca acaaactcaa tcgccatcaa catcccttta ccacgcgctt cctgtaccag
    29041 atcgggatat tcccgcgcca gttgacggaa accgtccagc aacatatcgc ctttttgctc
    29101 agcctgagcc ggtaagttct gctccagcaa cacattgatg gtcgccagcg ccgccgcaca
    29161 ggccagcggg ttgccgccaa aggtggtggt atgcaggaat gggttgtcga acagaactga
    29221 aaacacctct tcagtggcga tggtcgcgcc aatcggcatc acgccgccgc cgagggcttt
    29281 tgccagacac agaatgtccg gctgaacgtt ctcatgttcg caggcaaaca tcttgcccgt
    29341 gcgccccatg cccgtttgta cttcatcgag gatcatcagt gcgccgaact catcgcacag
    29401 cttacgcacg gcggtgagat agcccggcgg cggcagaatt acgccacctt cgccctgaat
    29461 cggttcgagg atcaccgcag ccacatcatc accggttttt ttgcactcat taagagccgt
    29521 gcgcatggct tcgatattgc caaacggcac atgacggaac cctggcagca gtggcataaa
    29581 cggtttgcgg aaggtcgatt tcgccgtggc cgacagcgcg ccaagtgatt taccgtggaa
    29641 cacgccgctg gtggcaataa aagtaaattt gccgcgcggc gactgataag ccttcgccag
    29701 cttcagcgcc gcttcaacgg actcggtgcc gctattacag aagaagctgt atttcagttt
    29761 accgggcgtt agcgcagcaa gggttttcgc cagcatagcc cgtaacggat cgagtagctc
    29821 ctggctgtgc agtggttgtt tcgcaagttg attctgtacg gcggaaacca caactggatt
    29881 acggtgcccc acgttgaaaa ttccaaaacc tcccaggcag tcgataaact cctgtccctg
    29941 ggtgtcgaca agcgtattta aacctcccgc ttgccactct acggctccgt aatccccgcc
    30001 ggcggtaaca gatttgcgat actctaaaaa ccccggattg acatgctctt tgaagtattc
    30061 aatcacctct cggttaagtg ctttcatctc ctcatgatcc agcgttcgct tctcaatgag
    30121 attcagggcg tgggcgctgc acgctaaagc cgatgcgctc gaaggtaacc tgttcaaaat
    30181 atgctccgga ggctcgcgta tcacatgata ccgatttaag tattgcaggg attgcgccac
    30241 tccggctgcg atcgcgaaaa tcgggataaa cacaaatgcg gttatcattt gtgcaatatt
    30301 taatgctatc cgttaacatt tgttgttatt ttgcacggtt tttgtcgggt atggatttga
    30361 tgattgctgc gcaaatgcac gttttcaggg caataattgc ctgatttttg ggcaagataa
    30421 ttaacgtttt cgtatagtaa cagagtgaaa gtattttaaa tcaaagaatt agatttgtgc
    30481 tttaatcgca aattgcgatc taaatcaaat taatcggtta aagataaccg cagcggggcc
    30541 gacataaact ctgacaagaa gttaacaacc atataacctg cacaggacgc gaacatgtct
    30601 tctcatccgt atgtcaccca gcaaaatacc ccgctggcgg acgataccac tctgatgtcc
    30661 actaccgatc tgcaaagcta tatcactcat gctaatgaca cttttgtgca ggtgagcggc
    30721 tttaccttgc aagagttaca agggcagccg cacaacatgg tgcgtcaccc ggatatgcca
    30781 aaagcggcgt ttgcggatat gtggttcacc ctgaaaaaag gggagccctg gagcggcatc
    30841 gtgaaaaatc gccgcaaaaa tggcgaccat tattgggtgc gggccaatgc ggtaccgatg
    30901 gtgcgcgagg gaaaaatcag tggctatatg tcgattcgta cccgggcgac ggatgaagag
    30961 attgcggcgg tggagccgct gtacaaagcg ttgaactccg gacgtaccag taagcgtatt
    31021 cataaaggcc tggtggtgcg taaaggctgg ctgggtaaac tgccttcatt accgcttcgc
    31081 tggcggacgc gtggagtgat gaccctgatg tttatcttgc tggcggccat gctttggttt
    31141 gttgctgccc cggtggtgac gtatttcctc tgtgtgttag tggtattgtt ggcaagcgct
    31201 tgttttgaat ggcagattgt gcgcccgata gaaaatgtcg cccgtcaggc actgaaggtg
    31261 gcgaccggag agcgtaatag tgttgagcac ctgaatcgca gcgatgagct ggggctgaca
    31321 ttacgcgcgg tagggcagct tggcctgatg tgccgttggc taattaacga tgtctcaagc
    31381 caggtgtcca gtgtcagaaa tggcagtgag acgctggcga aaggcaccga tgaactgaac
    31441 gaacataccc agcagacagt tgataacgtt cagcaaacgg tggcgaccat gaaccaaatg
    31501 gcggcgtcgg tgaaacagaa ctctgccacg gcgtcggctg ccgataaact ttctatcacc
    31561 gccagtaatg cggcagtgca gggtggggag gcgatgacca cggtgatcaa gacaatggac
    31621 gatatcgccg acagtaccta gcgcattggc accattactt cgctgattaa cgatattgcg
    31681 tttcagacca atattctggc cctgaatgcg gcggtggaag cggcgcgtgc cggcgaacag
    31741 ggcaaaggtt ttgcagtggt ggcaggggaa gtgcgtcatt tagccagccg cagcgctaat
    31801 gctgccaacg atattcgcaa gctgattgat gccagtgctg ataaggtgca atccggttcg
    31861 cagcaggtac acgccgccgg acggacgatg gaagatattg tggcacaggt gaaaaacgtc
    31921 acccagttga tcgcccagat tagccattca acgctggaac aggccgatgg gctttccagc
    31981 ctgacccgtg cagtggatga gcttaacctg atcacccaga aaaatgccga gctggtggaa
    32041 gagagtgcgc aggtgtcggc gatggtgaaa caccgcgcca gccgactgga agacgcggtg
    32101 acggtgctgc attaatcggt atgccggatc cggcggttgg agcacaatgc ctgatgcgat
    32161 gctggcgcat cttatcaggc ctgcggggtg tgtagcgggt gtaggccgga taaggcgcgt
    32221 caagcgtcgc atccggcaat tgcaccgcgc cactggcgga tgcggcgatt aatccagttg
    32281 cgtaatatca aaagcggcgc ggtcgatcac cgcaatgatc ttttttattt gtgcatcact
    32341 gatatcgctc tggttgacgc gtaaatccag caccgcttta aaattatcca gcgcccgctt
    32401 catttgcggg ttctggcgca gcgccgcgcc aacgcaacgc gctttgatgc gttcttcaat
    32461 catctccact tgttcgcggt tttcttccag ccactgcgcg ccttgttcgg tcagcgcaat
    32521 ctgcttctta ccaccttcct cttcgcggat ggtaatcagc gactgctcct gcagaaaatc
    32581 cagcgtcggg tagatgacgc ccgggcttgg ggtgtaattc ccctgggtta gattctcaat
    32641 cgctttaatc aattcgtaac cgtggctgtc atcgcgcgag agaatatcca gaatcaccag
    32701 acgcaattca ccgtgaccaa agaaacgttg ccgacgaccg ccgccgcgac cgtggcgacc
    32761 accgcaacat tgaccgtgtt catgctggtg accgtgtccg cagtgctcgt gttctgactt
    32821 ctcacctttg cagcagccct catggcgtgg ctggccttca tgtttacaac acccttcgtg
    32881 atgatggctc atatcagcct ccttttgctt gattagatat atctaattta tatctgatgt
    32941 gagcattact gcaagtacaa aaatgatttt taaacgtgga agcacctcac atctaggccg
    33001 gataagacgt tcacgccgca tccggcagca ttgctatggc actgaatgcc ttcacatact
    33061 tcagacatat cagttctaca aatcgcttgc atttatcatg attaacaatc attatcattt
    33121 gcgaatttta tttagatata tctgattacc atcacgaagg cgataacaat gaataacacc
    33181 ccccgctacc cgcagcgcgt tcgcaatgat ctgcgcttcc gtgaactgac tgtgctacgc
    33241 gttgagcgca tcagcgccgg ttttcagcgc attgtccttg gcggcgaggc gttggacggt
    33301 tttacatcgc gcggctttga cgatcacagc aaactcttct ttcctcaacc tgacgctcac
    33361 tttgtgccgc caacggtaac ggaagagagc atcgtctggc cggaaggacc acgcccaccg
    33421 tcgcgtgact atacgccact gtatgacgaa ctacgccatg aactggcgat cgatttcttt
    33481 attcacgacg gtggggtcgc cagcggctgg gcgataaact tacggtggca ggtccgcgcg
    33541 gttcgctggt ggtgccggaa gattacgcgt atcagctgta tgtctgcgat gaatccggaa
    33601 tgcccgcact gcgccgccgc ctggaaacgt tgagcaaact tgccgttaaa ccgcaagtta
    33661 gtgcgctggt tagcgtgcgg gataacgcct gtcaggatta tctcgcgcac cttgatggtt
    33721 ttaatatcga atggctggca catgatgagc aggcggtaga tgcgcgtctg gcgcagatgc
    33781 aaatccctgc cgatgat
//
LOCUS       DAJTUP010000034        33576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTUP010000034 DAJTUP010000000
VERSION     DAJTUP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.034"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..383
                     /locus_tag="OO468_001695"
     CDS             <1..383
                     /locus_tag="OO468_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6258845.1"
                     /translation="NTRTVQNWLSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTL
                     LPTIAGLSGLNNARFQFFNWMSGLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLP
                     VVLLVFGLAGSLVVLWKKKYGNRG"
     gene            387..770
                     /gene="mzrA"
                     /locus_tag="OO468_001696"
     CDS             387..770
                     /gene="mzrA"
                     /locus_tag="OO468_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR6258846.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            917..1271
                     /locus_tag="OO468_001697"
                     /pseudo
     CDS             917..1271
                     /locus_tag="OO468_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            1309..1614
                     /locus_tag="OO468_001698"
     CDS             1309..1614
                     /locus_tag="OO468_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR6258847.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            1617..2021
                     /locus_tag="OO468_001699"
     CDS             1617..2021
                     /locus_tag="OO468_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR6258848.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            2011..2310
                     /locus_tag="OO468_001700"
     CDS             2011..2310
                     /locus_tag="OO468_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR6258849.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            2496..2792
                     /locus_tag="OO468_001701"
     CDS             2496..2792
                     /locus_tag="OO468_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR6258850.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            2862..3848
                     /gene="yqjG"
                     /locus_tag="OO468_001702"
     CDS             2862..3848
                     /gene="yqjG"
                     /locus_tag="OO468_001702"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR6258851.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            4141..4509
                     /locus_tag="OO468_001703"
     CDS             4141..4509
                     /locus_tag="OO468_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR6258852.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(4716..5612)
                     /gene="yhaJ"
                     /locus_tag="OO468_001704"
     CDS             complement(4716..5612)
                     /gene="yhaJ"
                     /locus_tag="OO468_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR6258853.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            5717..6418
                     /locus_tag="OO468_001705"
     CDS             5717..6418
                     /locus_tag="OO468_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR6258854.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            6441..6605
                     /locus_tag="OO468_001706"
     CDS             6441..6605
                     /locus_tag="OO468_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258855.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(6817..8127)
                     /locus_tag="OO468_001707"
     CDS             complement(6817..8127)
                     /locus_tag="OO468_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR6258856.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(8155..9486)
                     /locus_tag="OO468_001708"
     CDS             complement(8155..9486)
                     /locus_tag="OO468_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR6258857.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(9761..11125)
                     /gene="tdcG"
                     /locus_tag="OO468_001709"
     CDS             complement(9761..11125)
                     /gene="tdcG"
                     /locus_tag="OO468_001709"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6258858.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(11197..11586)
                     /locus_tag="OO468_001710"
     CDS             complement(11197..11586)
                     /locus_tag="OO468_001710"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR6258859.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(11600..13894)
                     /gene="tdcE"
                     /locus_tag="OO468_001711"
     CDS             complement(11600..13894)
                     /gene="tdcE"
                     /locus_tag="OO468_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR6258860.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(13928..15136)
                     /gene="tdcD"
                     /locus_tag="OO468_001712"
     CDS             complement(13928..15136)
                     /gene="tdcD"
                     /locus_tag="OO468_001712"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR6258861.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(15162..16493)
                     /gene="tdcC"
                     /locus_tag="OO468_001713"
     CDS             complement(15162..16493)
                     /gene="tdcC"
                     /locus_tag="OO468_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR6258862.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(16515..17504)
                     /gene="tdcB"
                     /locus_tag="OO468_001714"
     CDS             complement(16515..17504)
                     /gene="tdcB"
                     /locus_tag="OO468_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR6258863.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(17603..18541)
                     /gene="tdcA"
                     /locus_tag="OO468_001715"
     CDS             complement(17603..18541)
                     /gene="tdcA"
                     /locus_tag="OO468_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR6258864.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            18731..19075
                     /gene="tdcR"
                     /locus_tag="OO468_001716"
     CDS             18731..19075
                     /gene="tdcR"
                     /locus_tag="OO468_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR6258865.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            19331..19871
                     /locus_tag="OO468_001717"
                     /pseudo
     CDS             19331..19871
                     /locus_tag="OO468_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            19893..20240
                     /locus_tag="OO468_001718"
     CDS             19893..20240
                     /locus_tag="OO468_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258866.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            20409..21059
                     /locus_tag="OO468_001719"
     CDS             20409..21059
                     /locus_tag="OO468_001719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258867.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(21567..21943)
                     /gene="rnpB"
                     /locus_tag="OO468_001720"
     ncRNA           complement(21567..21943)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO468_001720"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(21976..23121)
                     /gene="garK"
                     /locus_tag="OO468_001721"
     CDS             complement(21976..23121)
                     /gene="garK"
                     /locus_tag="OO468_001721"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR6258868.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(23218..24108)
                     /gene="garR"
                     /locus_tag="OO468_001722"
     CDS             complement(23218..24108)
                     /gene="garR"
                     /locus_tag="OO468_001722"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR6258869.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(24138..24908)
                     /gene="garL"
                     /locus_tag="OO468_001723"
                     /pseudo
     CDS             complement(24138..24908)
                     /gene="garL"
                     /locus_tag="OO468_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(24924..26258)
                     /gene="garP"
                     /locus_tag="OO468_001724"
     CDS             complement(24924..26258)
                     /gene="garP"
                     /locus_tag="OO468_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR6258870.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            26633..28204
                     /gene="garD"
                     /locus_tag="OO468_001725"
     CDS             26633..28204
                     /gene="garD"
                     /locus_tag="OO468_001725"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR6258871.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(28256..29080)
                     /gene="agaR"
                     /locus_tag="OO468_001726"
     CDS             complement(28256..29080)
                     /gene="agaR"
                     /locus_tag="OO468_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR6258872.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            29329..30609
                     /gene="kbaZ"
                     /locus_tag="OO468_001727"
     CDS             29329..30609
                     /gene="kbaZ"
                     /locus_tag="OO468_001727"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR6258873.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            30632..31105
                     /gene="agaV"
                     /locus_tag="OO468_001728"
     CDS             30632..31105
                     /gene="agaV"
                     /locus_tag="OO468_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6258874.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            31116..31895
                     /locus_tag="OO468_001729"
     CDS             31116..31895
                     /locus_tag="OO468_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR6258875.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            31885..32694
                     /locus_tag="OO468_001730"
     CDS             31885..32694
                     /locus_tag="OO468_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR6258876.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            32781..33215
                     /locus_tag="OO468_001731"
     CDS             32781..33215
                     /locus_tag="OO468_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR6258877.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            33212..>33576
                     /locus_tag="OO468_001732"
     CDS             33212..>33576
                     /locus_tag="OO468_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6258878.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLG"
BASE COUNT         8688 a         8693 c         8019 g         8176 t
ORIGIN      
        1 gcaatacccg caccgtacaa aactggctat ctcatttacc cgcgcattat catcaacgcg
       61 cacaccatct ttttcataaa cacggtttat cggcgctgtt aattggtcgc tttattgcat
      121 ttgtcagaac actgctgccg acgattgccg ggttatcagg gctgaataac gcgcgctttc
      181 agttcttcaa ctggatgagc ggtctgctgt gggtattgat cctgacaact ctgggttaca
      241 tgctcggcaa aacgccggta tttttaaagt acgaggacca gctgatgtca tgcctgatgc
      301 tgctcccggt ggtgctgctg gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa
      361 agaaatatgg aaatcggggg taagggatgc aaatacctcg catgtcgctt cgccagctag
      421 cctggtccgg cactgtttta cttctggtcg gcacgctgtt gctggcctgg tccgcggttc
      481 gccagcaaga gtctacgctg gcgattcgtg ccgttcatca aggcacaacg atgccagacg
      541 gtttttcgat ctggcatcac cttgacgctc atggcattcc tttcaaaagt atcaccccca
      601 aaaacgacac tctgttaatt acatttgact ccagcgacca gagcgccgcc gcaaaagcgg
      661 tcctcgacag aacattgccc catggctaca tcattgcgca gcaggacaat aacagtcagg
      721 ctatgcagtg gctgacccgg ttacgggata actctcatcg cttcggataa cttcctgtta
      781 tccgaaacat atcactcact ttggtgattt caccgtaact gtctatgatt aatgaggcgg
      841 tggttaacac taccgcttcg ttgtctggac aatcgttcct ttgtaatagg tccgaatcac
      901 aatggaaggt tcaagaatga aataccgcat cgctttagct gtttctctct ttgctcttag
      961 tgccggtagt tatgccacta ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat
     1021 cagctatgcc gaaaaacacc aaaaccagaa tcgtattgac ggtctgaata aagccctgag
     1081 tgaagtccgg gccaactgtt cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc
     1141 aaagcagaaa gatgaggtgg cggaacgcca gcaagattta gccgaggcga agcaaaaagg
     1201 cgatgccgat agaacggaaa ctggcagaag cgcaggaaga gctgaaaaag ctggaagcgc
     1261 gcgactacta actcacaata gtcactactt actcacctgg agaaaactat gtcgaaagaa
     1321 cacactacgg aacatctgcg tgctgagttg aaatcccttt ccgatacgct ggaagaggtg
     1381 cttagctcat ctggcgagaa gtcgaaagaa gagttgagta agattcgtag caaagcggag
     1441 caggcactga aacagagccg ttatcgcctg ggtgaaaccg gtgatgccat cgccaaacaa
     1501 acccgtgtcg cggcggcgcg tgctgatgag tatgtgcgtg aaaatccgtg gaccggcgtg
     1561 ggaattggcg ctgcaatcgg tgtagtgctc ggcgttctgc tgtcgcgtcg ttaattatgg
     1621 cggacactca tcacgcgcaa gggcccggta aaagcgttct gggcatcggg cagcgaattg
     1681 tttccatcat ggttgaaatg gtggaaacac gtctgcggct ggcggtggtg gagctggaag
     1741 aggaaaaagc taatctcttt caacttttac tgatgctggg cctgacgatg cttttcgctg
     1801 catttggtct tatgagcctg atggtgctaa ttatttgggc ggttgatccg caatatcgcc
     1861 tgaatgcgat gattgccacc accgtggtgt tgctgctact ggcactgatt ggcggtatct
     1921 ggacgttacg taaatcgcgt aaatccacct tgctgcgcca tacacgccat gagctggcaa
     1981 acgatcggca actgctcgag gaggagtccc gtgagcagta aagtcgaacg tgaacgacgt
     2041 aaggcgcaac tgcttagcca gatccagcaa caacggctgg atatttccgc cagtagtcgt
     2101 gaatggctgg aggcaacagg cgcttacgat cgtcgctgga atatgctgct aagtctgcgc
     2161 tcctgggcgc tggttggcag tagagtgatg gcgatctgga cgattcgcca tcctaatatg
     2221 ctggtccgct gggccagacg cggttttggc gtatggagcg cctggcgtct ggttaaaacg
     2281 accctcaagc agcaacagct tcgcggttaa cttccctctg gccggagcaa ttccggcctt
     2341 atccatcaaa ttttttgaat atttttgaca gttttccttg ctaacaatca tcattcacca
     2401 cgtttatgat tctctccatc gacagcaacg acgctaatag cgcgcccttg cacaaaaaaa
     2461 caatcagcag cctgagtggc gcagtggaga gtatgatgaa aaaattagaa gatgttggtg
     2521 tactggtagc gcacatttta atgccgattc tgtttattac cgcaggctgg ggaaaaatca
     2581 ccgcttactc tggtacccaa caatatatgg aagcaatggg tgtcccgggt tttatgctgc
     2641 tgacggcatt tttatttcac agcaactttg ctgaaggcgt caactcgctg atgttcatga
     2701 aaaacctgac aatttctggc ggattcctgc tgctggcaat taccggtccg ggcgcgtata
     2761 gcatcggccg cctgctgaat aaaaagtggt aagcacgcgc tatacttaac gataaaaaac
     2821 gaggaggaag ctcctcgttt ttgctattgg aggagagaaa aatgggtcaa ctgattgacg
     2881 gcgtctggca tgacacctgg tacgatacca aatctaccgg cggtaaattt caacgttcag
     2941 cttccgcatt tcgtaactgg ctcactgccg atggcgcacc tggccccact ggcacaggcg
     3001 gttttatcgc agagaaagat cgttatcatc tctatgtttc actcgcctgc ccgtgggcgc
     3061 accgcacgct gatcatgcgc aaactcaaag gactggaacc gtttatttcc gtttccgtag
     3121 tgaacccgct gatgctggaa aacggctgga cctttgatga cagttttccg ggagcaaccg
     3181 gagatacgct ctatcaacat gaatttctgt atcagcttta tctccacgcc gatccacact
     3241 acagcggacg agttactgtt cccgtgctgt gggacaaaaa gaaccacacc atcgtcagca
     3301 acgaatcagc agaaatcatc cgcatgttca ataccgcgtt tgatgcgctg ggcgcaaaag
     3361 cgggtgatta ctacccacca gccctgcaaa cgaaaattga cgaacttaac ggctggattt
     3421 atgacaccgt taacaacggc gtgtataaag ctggttttgc caccagccag caagcctacg
     3481 acgaggcggt ggcgaaagtg tttgaatcgc tggcgcgact ggagcagatt ttaggccagc
     3541 atcgttacct gaccggcaac cagctaaccg aagccgatat tcgcctgtgg actacgctgg
     3601 tgcgttttga tccagtgtat gtgacccact tcaagtgtga taagcaccgc atcagcgatt
     3661 acctgaatct gtatggcttc ctgcgcgata tctaccagat gccggggatc gccgaaactg
     3721 tcaatttcga tcacatccgt aatcattact tccgcagcca taagaccatc aaccctacgg
     3781 ggattatttc aattggtccg tggcaggatc tcgatgaacc gcatggacga gatgttcgct
     3841 tcggttaaaa aaaggcacct tacgaggtgc ctctgataat tagctaagtg catgttgttc
     3901 atgtcggatg cggcgtgaac gccttatcaa acctacaaag cgtttatatt caatatgtta
     3961 caatgaccgc gtaggccaga taagcgtagc gcatctggcg attttgcgct tgccatcagt
     4021 cttagcgcgc cttcacggca ctttattaat tccttgaacg aatatttact gccatttcat
     4081 tcacgtctat tcttaatttg ctgctttaaa aacaagtgat tgagcaaaat tgaggcaaaa
     4141 atggactggt atctgaaagt actaaaaaat tatgtcggtt tccggggtcg tgcgcggcgc
     4201 aaagagtact ggatgtttat tctggtcaac atcatcttta cgttcgtgct ggggctgctg
     4261 gataaaatgt taggctggca acgcgctggt ggcgaaggca tcctgacgat aatctacggt
     4321 attctggtgt ttttaccgtg gtgggcggtt cagttccgcc gcctgcacga caccgaccgc
     4381 tcggcgtggt gggcactgct attcttaatc ccgtttatcg gctggcttat tatcatcgtc
     4441 tttaactgcc aggcgggtac gccaggcgaa aaccgctttg gaccagatcc aaaactggaa
     4501 caagaataat gattaagcac cttgtaaaag gtgcttcaaa ttgttgacaa agtgcgcttt
     4561 gtttatgccg gatgcggcgt aaacgcctta tccagcctac aaattgccaa aattcaatat
     4621 attgcagtga ctgcgtaggc ctgataagcg tagcgcatca ggcagttttg cgtttgtcag
     4681 cagtcagaag caccttgtga aaggtgctta atagattatt ttccagcgaa caactttgga
     4741 atttcccgca gacaccagga tttcgcttcc cccatactgt cacgacgcca ggccataata
     4801 atatcgatct cgctggtcga ttccgggctg acgacacgca accgcccttc cgcaatatct
     4861 ttttcgacca tcggatacgg catcgtcgcc acgccaagcc ccgccagtaa tgcctgacgt
     4921 ttatcttcaa tcgtgctcac cgttaagcgc ggctgtttgt ccagcagctg tacggtcaac
     4981 accgggcgct cacgagcggt atccgctacc gcaattccac gatatttcac acgcgtcact
     5041 tcagataacg gttccggctc ctgatgaatc gggtgatcag gcgcggcaac gtagacgttc
     5101 attaacgtat agagcttgcg cgagttgatc tccgacgagg aacgaaaatg catatccggc
     5161 gcgataacga tatccgcccg cccctgctcc agccgttccc acgcccccgc cagcacttct
     5221 gtgatgattg ccagttgggt attggctttt gccgccagtt tgtcgattaa cgggaaaaag
     5281 gcaggtgtcg gtaccagcgc ttcggtcaca atggtgagat gcgtttccca accgcgcgcg
     5341 agagcttccg catcagtagt cagtttatct gcggcttcca gcaaaacgcg ccctcgctcc
     5401 agcaacatcc tcccgacatt ggtgaatttg gtgcgatggc ccgagcggtc aaacagcacc
     5461 acatccagct cttcttccag tttttgcatg gtgtagctaa gtgcggaagg cacgcgtccc
     5521 agctcatccg ctgccgccgc aaaactgccc cggcgatcga tcgcatccat aacccgtagt
     5581 gcttccagcg ttaatgccct ttctttggcc atttcgttct cattcaggaa atttgaacat
     5641 accgggcaga atatctggct aacaatgtag cgtccagccc cttaccataa aaggaagtaa
     5701 agagaggtca agaattatga ttactacccg aactgccagg cagtgtggac aagcagacta
     5761 cggatggttg caggcccggt atactttttc ctttggacac tacttcgacc cgaaattgtt
     5821 aggctatgcc tccctgcgtg tgcttaacca ggaagtgctg gccccaggtg ccgcctttca
     5881 gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt ctggatggag aagcagagta
     5941 tcgcgacagc gaaggcaatc atgttcaggc cagcgccgga gaggcgttgc tgctctctac
     6001 ccagccgggt gtcagctata gcgaacacaa tctcagcaaa gacaaaccgt taacgcgaat
     6061 gcagctttgg ctggatacct gcccgcagcg agagaatccg ctgattcaaa agctggcgct
     6121 taatatggac aagcagcaat taatcgcctc gccagagggt gcgatgggaa gcctgcaatt
     6181 acgccaacaa gtgtggctgc accatatcgt gctcgacaaa ggcgaaagtg cgaatttcca
     6241 gttgcatggg ccacgcgcgt atttgcaatc gattcacggg aaatttcatg cgcttacgca
     6301 tcatgaagag aaagcggcgc taacctgcgg tgatggggcg tttattcgtg acgaggctaa
     6361 cattacgctg gttgccgatt ccccactgcg cgctttgctg atagatttgc ctgtctagtt
     6421 gtttttacag ggagatgatg atgagtaaaa aatcgaccaa aaagcgccag ccagcgaagc
     6481 ccgtggtggc gaaagaacct gctcgcaccg ccaaaaattt tggctatgaa gagatgttga
     6541 gcgagctgga agctatcgtc gcggatgctg aaacgcgttt agccgaggat gaagctaccg
     6601 cgtaaagaca ggttgccgga tgcggcgtaa acgccttatc cggcctacaa aacacaacaa
     6661 catcaacaaa ttaaacaccc cgtaggccgg ataagatgcg ccagcatcgc atccggcatc
     6721 aggtacaaaa cacaacaaca tcaacaagtt gagcaccccg taggccggat aaggtgcgcc
     6781 agcatcgcat ccggcacaat actcaaaacc tggcgtttat ctggccttgc tcgccataat
     6841 ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc
     6901 aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc
     6961 cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcgca
     7021 gctgttcgac gcaccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc
     7081 catcgagata gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat
     7141 tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat
     7201 tgcgctcaaa tgagaaagca tcagcgcacg cgccagtcgt tcatcatccg ctccgaagtg
     7261 ttctgctaca accaccacag gcattattgc agtgatcccc tggttacccg agccggagtt
     7321 actcatagcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat
     7381 cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt
     7441 cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag
     7501 cttcgcggaa tcgagaataa agcggatcgc cgcaaatggg acttcattga cgaacttcag
     7561 gatctcagcc agcgtcgttc tggaaagcac cgtcagcggc gactcttgct cgccctctgc
     7621 cacacacgcc tggtgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt
     7681 atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga
     7741 gaagaggatt tcattgcaag gttcctggat cttaacagag actttccccg ccgccagcag
     7801 tgctttggca tcggtaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt
     7861 ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc
     7921 gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg
     7981 ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg
     8041 ttcagtacat cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca
     8101 taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga
     8161 aaatgccagg aacagagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga
     8221 catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca
     8281 ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag
     8341 tacgatggcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt
     8401 ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt
     8461 tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag
     8521 aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat
     8581 cgccagcgcg gaaatattct gctcgtaggc ttttaccgct tcgtcatgtc ccatcgccag
     8641 cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat
     8701 tgcccgcaat gctttatgcc gcgccacttc aattgatttt tcccgcgagc gataagagat
     8761 caccatcggg cttaacgtct ggataaacag aatcgacgtc agggtaaacg gcagcgtaat
     8821 aatggcgttt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca
     8881 cattcccacc atcgacacgc ccagcgcagc gactaccagc agcttggtca gcaccatgtc
     8941 ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat
     9001 cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg
     9061 cagataggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag
     9121 cattacgaaa tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat
     9181 gacactcggg taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg
     9241 aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat
     9301 caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat
     9361 aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact
     9421 cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat
     9481 ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa
     9541 ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg
     9601 aggtcagaga aaatcttcac aatcaagact ttaaaataac ggagatggat aagaattttc
     9661 tactaaatta atcacaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg
     9721 tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa
     9781 tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat
     9841 acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca
     9901 tccgcgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt
     9961 gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg
    10021 ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgctgcca
    10081 ttgaacaggc cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt
    10141 tcattttata cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct
    10201 cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg
    10261 caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca
    10321 gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctag
    10381 aaaccagctg acgacgcagc gctacggcac gacacggcac attgagcgga ccaggcagca
    10441 cgccttcagt gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa
    10501 accctgcgtc aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag
    10561 atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt
    10621 aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc
    10681 cgccgccgac agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga
    10741 tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta
    10801 caggaaaatc aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta
    10861 actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc
    10921 ccatgataat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccgtaca
    10981 gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa
    11041 aactttttcc ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt
    11101 tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga
    11161 gcggaacgac cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc
    11221 ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg
    11281 gtaggtcgcc tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc
    11341 attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc
    11401 aaccacgatc gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg
    11461 gatctcaccg gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa
    11521 atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt
    11581 tttcatacat cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt
    11641 gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt
    11701 taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga
    11761 cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc
    11821 cgacaaggtt ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga
    11881 aggtgtacga gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg
    11941 aggccacggc acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg
    12001 ttccggcgcg acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag
    12061 tgatagtcag aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt
    12121 tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc
    12181 cgtactgcgg atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga
    12241 ttggtttcac gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg
    12301 cgatgccgca tgccatagtg cgatagacat cacgatcgtg cagcgccatc agcgaagctt
    12361 cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg
    12421 ccagccagtc catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt
    12481 ccatcagcgg tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt
    12541 taattgcgta gagcagcgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct
    12601 taccaatcac cattgggctg acgcagcagg caatcgcgta atcgtcgctg ttgaagtcag
    12661 tacgcatcag atcgtcattt tcatactgca aggaagaggt gacgatcgac acctgcgcgg
    12721 catatttttt gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg
    12781 gtgccggccc catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg
    12841 tacgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga
    12901 acagcgaatc aaattccggt gtacgcagga agcgtaccat acggatcttc atgatgaagt
    12961 gatcgatcag ttcctgtgcc tgctgctcat tgaggacgcc agctttaaag tcgcgctcaa
    13021 tgtagatatc caggaacgat gccgtgcgcc ccagcgacat cgcgccgcca ttttgcgatt
    13081 tcactgccgc cagataagcg aagtagagcc actgcaccgc ttcctgtgca ttctgcgccg
    13141 ggcgagagat atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac
    13201 gatgctctgc cagctcctca cgcagacgga tggtggcttc cagatcctcg cctttttcca
    13261 gacgagactg gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat
    13321 acagcgctac gcggcgatag tcaccgataa tgcgcccacg accatagcca tctggtaaac
    13381 cggtcagcac gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc
    13441 cctggttatg ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac
    13501 ggccataggc gtggaatgaa cttttaatca tattaatgcc accgaacggg tgtagcgcac
    13561 gtttcaacgg cgcatccgtt tgcaggccaa caattttttc cagcggctgg ttaatatatc
    13621 ccgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg
    13681 ttgcgttttc gatacggatg ccttccatta ccttttccca caattccgtg gtggcaggcg
    13741 tcgcttcggc gagaaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat
    13801 cgcggacatt aatttcgctt ttccagtccg tacctttaaa gccaagccat gcgtcagcgt
    13861 acagcttatc gctggtatca atatctacct tcatgaaaaa taatctctct acaatacttc
    13921 aactaaatta tgcaaattct gcgggcgcgt taactttacc taaatgaatg gcatccaaag
    13981 caatcatttt ttcttcgtta gtcggaataa cgacgcaaat gacatgcgca ttttcactgg
    14041 aaacaattcg ctcgccaaaa gagttagaac gattattcat ttctgtatca atctctacgc
    14101 ctaatacagc caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc
    14161 cgccggtgaa tataattcca tccagccgac gtaatgaagc tgcatgtccg gcaatatgac
    14221 gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc
    14281 aggctttttc cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact
    14341 ctttattcac tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg
    14401 acatcgcacc aaagtcgaca tcgccactgc gggtacccat catcaagcct tccagcggcg
    14461 tcattcccat tgaggtgtca acactctgac cgttgcgaac tgcgcagatt gacgcgccat
    14521 tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc agcgaatgtg
    14581 cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact
    14641 cttcataata tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga
    14701 aactggtatc aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa
    14761 taccacttaa atttgcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat
    14821 caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc
    14881 ggtggccaat taaggccacg ctgtcattta aattccgttt ttccagttca aatgcaattg
    14941 ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg
    15001 ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat acttcacaat
    15061 cgctggcatc gagtacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa
    15121 ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg
    15181 atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg
    15241 tatttcgcca gagacggcgc tttacggata gcatacatcg gcaacaggca cagcagggat
    15301 gcgataatcg gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca
    15361 acaacccagg tggagcccat gatgaagatc atgctgagag tattcagttt acccagcgac
    15421 actttggttt tgtcaccttt ataaccgaac ttcagaatca gaccattcaa gccttccagc
    15481 gtccccagat agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc
    15541 gcatattcca gtgtaatcgc gaacgttgtt ttcgtaccgg tcatggacgc aaagtggtta
    15601 gccagataag aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac
    15661 agagtaaaca ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca
    15721 cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac
    15781 tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaagttaaa ggagaaaacc
    15841 atgatggaaa tccccagcca gacagtgatc aggataccgt catgaccggt taacgacagc
    15901 gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa
    15961 atcagcacca ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga
    16021 tccttaccaa accagatgac gaaagccatc agcagcaaca ggaacagcgc cacaaagccg
    16081 cgattcagcg gtgcaaatcc gagctggttt tcccagaacg tcataaaggt attggtaata
    16141 gtaacgccat aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc
    16201 acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta
    16261 gagccagaaa gacacagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc
    16321 aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac
    16381 agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta
    16441 cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat
    16501 cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg
    16561 atattgccgc cggaaataat actgacggtt tttctatttt gaatatattg gtctaattta
    16621 ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc
    16681 tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg
    16741 actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc
    16801 gccagggtgc cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg
    16861 ccgtgaacgt tttctgactg tacgccaata acacgaatgg tcgggttaat agatttaatt
    16921 gccaccgcaa taccagcaat taaaccacca ccaccaattg gcacaatcac gttatcgaca
    16981 tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga
    17041 tcatcgtaag gtgggataaa aatacgacct tccatttcga caatttcgct cactttagcg
    17101 atagtgtcgt tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc
    17161 gctactttgg attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc
    17221 gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg
    17281 cgtttttccg catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa
    17341 cccgtacgct gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa
    17401 tagttggagc gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca
    17461 ataatatcat caatagcaac cggcagatcg tatgtaatat gcattataaa acctcttcgc
    17521 ctgaattaaa tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt
    17581 atgtttaaat aaaaacaaac aactaaccaa cttctattaa ttgccttcgt ctacacccat
    17641 tataagatga atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat
    17701 aatttttcga ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag
    17761 taataaattg attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat
    17821 cagcattgag aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa
    17881 tactgatgcc atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt
    17941 gtggcaacac ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc
    18001 gggacttact ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct
    18061 gaagcttcat ttctgcactt aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg
    18121 ccggtaagaa cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct
    18181 ctttgaactt gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa
    18241 atgagacttc caccaccgct tcagaagaca taccgcttat ctcattaacc atatttttca
    18301 tttcacgggt aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta
    18361 caccggtatt tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga
    18421 ttttactgac ggccggttga gttaacccta attcttttgc agccgagccg atagagccac
    18481 ttctaatgac ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca
    18541 taacgaccta cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaataaacag
    18601 gatatgtgcg accactcaca aattaacttt cattactttc caaggtatcg ttattacaat
    18661 taaacatctt taaatatcaa caagttaaag cataaaaatc agcataaacc ctgattttta
    18721 tcaaaaaaaa atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt
    18781 taatacaaaa aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa
    18841 atttgagtta aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc
    18901 gataaatact gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt
    18961 ttatcaatat tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt
    19021 caactttttc tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct
    19081 tctcccctca taggcgacga atagcatttt gtgttgagga tcacaaaacg aataattgct
    19141 ggtcgccgcg ataaggtcag acgaagtcaa caagggggat tttcactgtg ctttacatca
    19201 gcgtaggaca cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga
    19261 tagtgcgtaa gtgtcgtgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag
    19321 gcaggcatca atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat
    19381 gcacgcagtg gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt
    19441 tgcagaaatt gtctccacca atgacgttaa taaaatttac agtataagta atgaactccg
    19501 gagagtatta tctgcaatta ctgcattgaa tttctatcaa ggcgatgttc cttctgtcat
    19561 gattccgaat ccaaccggaa aatatgagtc cattcatcat agatatttct acaggagaac
    19621 atgatgatta tatcatacaa acattagatg taggcacttt tgcacctttt ggtgaacaat
    19681 gtacttgctc agccgtcaat aaaaaagagc tggaatgtat taaggagacg atttctaagt
    19741 attgcgcaaa gttcacccaa aaagaagcca tcttaacacc ccctaggcac tttaataaaa
    19801 cctgcattac ttcagattgt tggcaaattc tctttttttc acccgatcat tttaataatg
    19861 atttttattg attatttata aaggataaaa taatgttccc tgtttcatca attggtaatg
    19921 atattagtag tgatttagtc cgtcgaaaaa tgaatgacct tcctgaaagc cccataggaa
    19981 ataacctcga agcactagca ccaggtatag aaaaactaaa acagacctct attcaaatgg
    20041 tcactttact taacgcgtta caaccaagtg gaaaatgcat tatcactggt gattttcaaa
    20101 aagaattagc gtacttacaa aatgtaattc tttataatga ctcgtctctt cgtctggatt
    20161 ttttaggtta taacacccaa attattcaac gatcggacaa tacctgcgaa cttaccatta
    20221 atgaaccgtt aaaaaactag gaaatatcta caggcaatat caatgttaat tgcccattaa
    20281 aagatattta caatgaaatc aggaggttga acgtaatctt tagttgtggg actggagata
    20341 tagttgatct atcctctctg gacttacgta atgtcgattt agattattat gatttcacag
    20401 ataaacatat ggctaatact attctaaatc attttaaatt ggattcaaca aattttacta
    20461 atgccaacat gtttcaggtt aattttgtta gttcaacaca aaacgccaca atctcctggg
    20521 attatttact aaaaataacg cctgttttaa taagcattag cgatatgtat tctgaagaaa
    20581 aaatcaagtt tgtcgaaagt tgtttaaatg agcttggaga cattaccgaa gaacaattaa
    20641 aaattatgag atttgcaatt ataaaatcta taccaagggc aactcttaca gataaattag
    20701 aaaatgaatt aacaaaagaa atatataaaa gctcatcgaa aatcaacaat tgcttgaaca
    20761 gaattaaatt accagagatg aaagaattct catcaaaaaa aacatatgat tacatcgata
    20821 caatcattga agattatgaa aatatcaaag aaaatgctta tctggtcgtc ccccaaatta
    20881 attatactat ggatttaaac atagaagact ctagctcaga agagttaata ccctcgagaa
    20941 agacgaaaat tctccggaca atggctttga ggtcggggaa tataacacat atgaagcata
    21001 taactcagag aatcaatatt ttaccagaga ggactatacg tataattacg accttttaaa
    21061 tgcaatatag acttaataac ttcttacaaa gtctattatt aataccgtag atattcatct
    21121 taccctgtgc accaaaagca aaaacttatc gacgcgggtt tgaaactgat ggcaaccgca
    21181 aaaatgcctg atgcgctacg cttatcaggc ctacgccatc tctgcaatat attgaatttg
    21241 cgtgcttttg taggcaggat aaggcgttca cgcagcatcc ggcatgaata aagcgcactc
    21301 aacaatctga aacccgccgg aacggtttat tacgtacatc aggtgaaact gaccgataag
    21361 ccgctttctt ttgggtatag tgtcgtggac agtcattcat ctttctgccc ctccaaaagc
    21421 aaaaacccgc cgaagcgggt ttttacgtat atcaggtgaa actgaccgat aagccgcttt
    21481 cttttgggta tagcgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc
    21541 cgccgaagcg ggtttttacg taaaacaggt gaaactgacc gataagccgg gttctgtcgt
    21601 ggacagtcat tcatctaggc cagcaatcgc tcactggctc aagcagccta cccgggttca
    21661 gtacgggccg taccttatga acccctattt ggccttgctc cgggtggagt ttaccgtgcc
    21721 acggactgtt accagccgcg cggtgcgctc ttaccgcacc ctttcaccct tacctgatcc
    21781 cgcttgcacg ggccatcggc ggtttgctct ctgttgcact ggtcgtgggt ttccccccca
    21841 ggcgttacct ggcaccctgc cctatggagc ccggactttc ctcccctcca cccgtctccc
    21901 ccgaagagga cgacgacgaa gcggcgactg tctggtcagc ttcggcgcgc agtatagagg
    21961 gtttgcgcgc ccttgtcacc ccgtgttgcg cattccaatc gccagtgtcg cggcaatatt
    22021 acgtgaagca cggcagatat tgtcataagc cccgcggaat gcttcgtcca acgtacctat
    22081 gctggtcaat acgctgaaga ccgcatcaat gccatgctga tgtacaacgc caacatcatc
    22141 ggtcaggcta cccgcaatgc caatcaccgg tttatggtac ttcttcgcca cgtttgcgac
    22201 gccaatcggt accttgccgt gaatactctg gctgtcaata cgcccttcac cggtgatcac
    22261 cagcgtacaa tcgtgaatat gttcctccag attcagcgcc gtagtgacga tttcaatacc
    22321 acttttcagt tccgcaccaa gaaacgccat cagcgccgca cccataccgc ccgcagctcc
    22381 tgcgccgggg acatctttta catcaacatg cagcgctttt ttaatgacat cggcatagtg
    22441 agagaggtta ttgtccagct caacaatcat cgcttcactg gctccctttt gtgggccaaa
    22501 gatgcgcgat gcgccgttat cgcccaccag cggattggtg acatcacaag cgacgcgaat
    22561 gacgcaatct tttaagcgcg gatcgaggcc ggaaatatca atatcattca gagtattaag
    22621 actaccgccg ccaaaaccaa tttcattgcc gttggcgtcg cataatttcg cccccagcgc
    22681 ctgtaccatg cctgcgccgc catcatttgt agcgctaccg ccaatgccga taataatgtt
    22741 tgtcgcgccg ctctccagcg cctgcaggat taactcgcct gtgccgcgtg aagtggtcac
    22801 gagtggatcg cgtttttccg caggtaccag ctccagccca ctggccgccg ccatttcaat
    22861 aaacgcggtt ttgccatcgc cggagatccc ccaactggca ttcactttct cgcccagcgg
    22921 ccctgtaacc caggcgtgac gttcagaacc ctgggtggct gcaatcatcg cttccaccgt
    22981 tccttcgcca ccgtcggcaa ccggaataga aacgtactgt gcatcaggaa aaatttcccg
    23041 aaatcctttt tctatcgcct gcgcaacctc gctggcagat aaactttctt tataagagtc
    23101 tggggcgatt acgattttca tacctatgcc tgttaccaca tgacgccgga gggcgtttct
    23161 cttattcggc ctggattcca ggcccggatt gcaatacgcc atccgggcac gacgtcatta
    23221 acgagtaact tcgactttcg ccagtttttc gtagtagcac accagggcgc tatgatccgc
    23281 cgttcctaaa ccatctgctc gcagtgcctg catcatctcc ataaccgcag ctgtgagcgg
    23341 cagttgcgcg ccgacgccgt gggaagtatc cagcgcattt gccagatcct taatatgcag
    23401 atcaatacgg aagcccggct tgaagttgcg gtccatcacc atcggcgctt tggcatccag
    23461 cacggtactg cccgccagtc caccgcgaat tgcctgatac accaggtccg ggttaacgcc
    23521 cgctttagtt gccagcgtta acgcttctga catcgcggca atattcagcg ccacaatgac
    23581 ctgattcgcc agtttggtga cgttacctgc accaattgcc ccggtatgca ccacggaacc
    23641 cgccattgct ttcatcaaat catagtattt gtcgaaaata gccttgtcgc cgcccaccat
    23701 cactgacagc gtaccgtcga tggctttcgg ttcaccgccg ctcaccggag catccagcat
    23761 atcaatgcct ttcgctttca gcgcttcgct gatttcacgg cttgccagcg gtgcgataga
    23821 actcatatcg atcaataccg tacctggctt cgcgccttca ataatgccat tctcacccag
    23881 cgccacctct ttcacatgag gggagtttgg cagcatggtt atgatgacgt cgcactgttc
    23941 agcgatcgct ttagccgtag aggctgtttc tgcacctgca gcaatcacgt cagcaatagc
    24001 ttctgggtta cggtcagcaa ccaccagcga gtaacctgct ttcagaaggt ttttactcat
    24061 tggtttaccc ataatcccca ggccaataaa accaactttc atagtcatat caatcatctc
    24121 tcttgttgcg gtggtggtta ttttttaaag gtatcagcca gtttatgagt ggcagagcgg
    24181 aagacgccga gatcgctgcc gacagccaca aacgtcgcgc cccattccag ataacgacgc
    24241 gcatcggctt cgaccggcgc gaggataccg ctgggtttgc cgtgcgcgct ggcacggtta
    24301 aaaatgtgct gaattgcttt ttgtacatcc gggtgtgatg cattgccgag atggcctaat
    24361 gccgcggcca gatcgctggg gccgacgaag atgccgtcta cgccttcggt agcggcaatg
    24421 gcatcgacgt tatctacgcc ctgctgactt tctatctgga ccagaatagt gatgttcttg
    24481 ttcgactgag cgaaataatc cgccacggtg ccaaacatat tggcgcggtg agaaacggag
    24541 acgccgcgaa tgccttccgg tgggtaacgg gttgatgcca ccgcctgctc tgcttcctct
    24601 tttgtttcta caaaaggaat caggaagtta tagaaaccga tatccagaag acgcttaata
    24661 atttccggct cgttggtcgg cactcacact actggcgcgc tggcgctgcc tttcaaggcc
    24721 attaactgcg gaataaacgt ggagatatcg tttggcgcat gttcgccatc cagcaccagc
    24781 cagtcaaacc cagccaaacc aagaacttca gtgctaatcg ggttagagag tgctgaccag
    24841 caaccaattt gtacctgttt cgcagccagt gcggctttga atttattcgg gaaaacatcg
    24901 ttattcatcg cttatacctt tgcttatttc tgcaattcca tacgtttaat gtcgccaact
    24961 acgaagaggt agcagaccat cgccatcagc gctgaacatc ccacgaaaac cagtgctgca
    25021 ttgaaggagt gcagttcact taccaggtag ccaatcacca gtggagtgac aatggaggca
    25081 acattgccaa agacgttaaa gacgccgcca cagaggccaa caatctcttt cggcgcggtg
    25141 tcagaaatca ccggccagcc cagagcacca aatcctttgc caaaaaaagc cagcgccatc
    25201 agcatgacca ccagcgtggt gttgttggtg tagttacata agatgatggt ggaagccagc
    25261 aacattccca gcacaatcgg tagcttacgt gccagggtca gggataaacc gcgtttgatc
    25321 agataatccg agaagacacc tcccagcacg ccgcccgcaa aaccacacag tgccggaatc
    25381 gaggcgacca gacccacttt cagaatcgac atgccttttt cctgcaccag ataaatcggg
    25441 aaccaggtga ggaagaacca ggtgatggtg ttgataaaat attgtccgaa aaatacgccc
    25501 aacatcatgc ggttagagag caattgcttg atgtaatgca gtttgggacc gcttgctgcc
    25561 gcgctgcccg gctttttgtg gtccatatcg accaccgcgc cattttcaga gataaacttc
    25621 agctcttcct cagacatacg tgggtgatct gtcgggttat gaatcaactt gatccacagc
    25681 gccgtcagca caaaacaaat cacccccata acggtaaaga cgtgctccca tccccaggcg
    25741 aaagtcagcc agccaagcag aggcgaaaag agcgccagcg agaaatattg tgccgagtta
    25801 aagatggcgg aggcagtacc acgttctttc gtcgggaacc aggcggcgac aatacgggcg
    25861 ttcgccggga aggatggagc ttccgagaat cccagcataa agcgcataaa gaacatggag
    25921 atccctgccc aagccagcgg gaacatatca acaaagcctt gcaggaaggt gaacagcgac
    25981 cagaaaaaga ggctgtaggt gtaaactttt ttcgagccaa acttatcaag caaccagccg
    26041 ccggggattt gcatcagcaa gtaggcccag ccaaaagcgg agaagatgta acccatcgaa
    26101 accgcactta actgcaactc ttttgccact tcggtaccag caatagacag cgttgcacga
    26161 tcggcgtagt taacggcggt aacaataaaa ataatcagta atattaaata gcgggtatgc
    26221 acacctttcc ttttttcgtc aacggtgtcc agaatcattt tatttacctc gggtacttat
    26281 gctgattttt attattatgg ggaaggtgtt atttataagt ttcatttatg ccgtaacgac
    26341 aatgaactcg ggaattagta taagcagcgc gagaataata atcattgtgc aaatgctaat
    26401 ttaattaata ctttttaaat attattttga gcatatgcac ataaggttgc gcgctaaagc
    26461 acagatttgc gctttacctt gccgggcggc actgcaatcc ctaaaatgat tgacattgat
    26521 cacatttctg cgtttaaact cctgacattc ttatttcacc caatgaaatc atttattttt
    26581 aaatgagacc aggtcctcat tttaataacc cctggctgga gaatattgca caatggccaa
    26641 catcgaaatc agacaagaaa cgccaactgc gttttatata aaagttcacg acacagataa
    26701 tgtggcaatt attgttaatg ataatggcct gaaagcagga acgcgttttc cggatgggct
    26761 ggaattaatt gaacatattc cccaggggca taaagtcgca ttgctggaca ttccggctaa
    26821 tggtgaaatt attcgttatg gcgaagtgat tggttacgcc gtgcgtgcaa tcccacgcgg
    26881 aagctggatc gacgaatcaa tggttgtact accggaagcg ccgccgttac acacgctgcc
    26941 actggcaacc aaagtcccgg aacccttacc gccgctggaa ggatacacct ttgagggcta
    27001 tcgcaatgcc gatggcagcg tgggcaccaa aaacctgctc ggcatcacca ccagcgtcca
    27061 ctgtgtggca ggcgtggtgg actatgtagt aaaaatcatt gaacgcgatc tgctaccgaa
    27121 atacccgaac gtcgatggcg tggtggggct gaatcatttg tacggttgtg gcgtggcgat
    27181 taacgcaccg gcagcagttg tacctattcg taccattcac aatatttcgc tgaaccctaa
    27241 ctttggcggc gaagtaatgg tgattggcct gggttgtgaa aagttgcagc ctgagcgcct
    27301 gctgactgga acggatgatg tgcaagctat tccagtagaa agcgccagca ttgtcagttt
    27361 gcaggatgaa aagcatgtcg gttttcagtc catggtcgag gatattttgc aggtcgccga
    27421 acgccatcta caaaaactga atcaacgtca gcgagaaacc tgtccggctt cagaactggt
    27481 tgtcggcatg cattgcggtg gcagcgatgc gttttctggc gtaacggcaa acccggcggt
    27541 tggctatgcg tctgatctac tggtgcgctg cggcgcaacg gtgatgtttt cagaagtcac
    27601 ggaagtgcgt gacgcgatcc atctgctgac accacgcgca gtgaacgaag aggtcggcaa
    27661 acggctgctg gaggagatgg agtggtacga taactatctc aatatgggaa aaaccgaccg
    27721 cagcgccaac ccttcgccgg gcaacaagaa aggcggtcta gcaaacgtgg tagagaaagc
    27781 gctcggctcc attgctaaat cgggcaaaag tgcaattgtt gaagtgctgt cgcccggtca
    27841 acgcccgact aaacgcggat taatttacgc cgcgacgcca gccagcgatt ttgtctgtgg
    27901 cacgcaacag gtggcttcgg gtatcaccgt gcaagtgttt acgaccggtc gtggtacgcc
    27961 gtacggcctg atggcggtac ccgtcattaa aatggcaccc cgcaccgagc tggcgaaccg
    28021 ctggtttgat ttaatggata ttaatgcggg caccatcgct accggcgaag aaactattga
    28081 agaggagggc tggaagttgt tccactttat tctcgacgtc gccagcggga agaagaaaac
    28141 cttctcggat caatggggac tgcataacca gctggcggtg tttaacccag caccggtgac
    28201 ctgataattc aattcgtatt atgcccgata gcacacgttt atcgggcata ttgagtcaca
    28261 gcgaagacgc gtgatccccg accagaatca cttcaacccc agcctttcgc agtccttcca
    28321 gactatccgc aggaatgcct tcatcaacaa tgatcatgtc gatacgttga gtatcaatga
    28381 tcttatgtaa actggaacga ttgaatttac tggaatcggt gaccacgatg atccgttccg
    28441 caacttcgca catccgacgg cttaaacggg cttcatcttc attatgtgtg ctgacgccgc
    28501 gctccagatc gatcgcatct acaccaagaa acagcatatc gaagtggtaa ttttgcagcg
    28561 attgctcagc ctgatcgccg taaaaagatt gcgactgacg gcgcaaatgc ccgccggtca
    28621 tcagcagctc aacgccttcc gcttccagca acgcattagc cacgttcata ccgttggtca
    28681 tcgcaattac gtcagtgtac ttgcgcatca gacgagcaat ctcaaaagtg gtggtcccgg
    28741 aatcgaggat cacccgatga cctggctgaa tcaactcaac ggcagctttc gcaacgctgc
    28801 gtttcatcgc ggtgttcagt gcgcttttat cttccactga tggctcgact gacggcgtcg
    28861 tgctatcgca gatcaacgcg ccaccatagg cacgcacagc gatcccctgc ttttccagaa
    28921 acgccagatc attgcggatc gtcacagtag atacgccata caatgccgac agatcgttaa
    28981 cctgcacact cccttgctgt cgcagacgct gaatgatctg ttctcgtcgc tcgctggtgc
    29041 ctgtcactcg cttctcacct gaagcgtcgg tattactcat agtaagtcct ttcgtaaaac
    29101 tttcgtttca tttcgttttg cctattaacg cctttctatt aagcaaatgc aagcccacct
    29161 tgcccattgg cgcaagctac tctcgtttca gtgactttca ttacgtttct tttgtgaatc
    29221 agatcagaaa actattatct ttcgttttat ttttatctca ccatgacgca gtatcaattg
    29281 aaacaaaacg aaagatcaat atcgcagtaa tctgaactgg agaggaaagt gaaacatctg
    29341 acagaaatgg tgagacagca caaagcgggc aaaacaaatg caatttatgc cgtttgttcc
    29401 gcacatccgc tggtgctgga ggctgcaatc cgctacgcca gtgcaaacca aacgccgtta
    29461 ctgattgaag caacctccaa tcaggtagac cagttcggcg gttataccgg aatgacgccc
    29521 gccgattttc gcggctttgt ttgtcagctc gccgactcgt tgaatttccc gcaggatgcg
    29581 ctgattctgg gtggtgacca tctggggcca aaccgctggc aaaacctgcc agccgctcag
    29641 gcaatggcca atgccgatga tttgattaaa agctacgttg cggcaggatt caaaaaaatc
    29701 caccttgatt gcagcatgtc ctgtcaggac gatccaattc ccttaactga tgacatcgtg
    29761 gctgaacgcg ccgcccgtct ggcgaaagtg gcggaagaaa cctgtcgcga acactttggc
    29821 gaagccgatc tggagtatgt cattggtacc gaagtgccgg tacctggcgg cgcgcatgaa
    29881 accttaagcg agctggcggt caccacgccg gatgccgccc gcgccacgct ggaagcccat
    29941 cgtcacgcct ttgaaaagca aggtttgaat gccatctggc cacgcattat tgccctggtg
    30001 gttcaacccg gcgtcgaatt cgatcacacc aacgttattg attatcagcc cgccaaagcg
    30061 agcgccttaa gccagatggt cgaaaactac gaaacgctga ttttcgaagc gcactctacc
    30121 gattatcaaa cgccgcaatc tctgcgccag ctggtgattg accactttgc cattctgaaa
    30181 gttggcccgg cgctgacctt cgccctgcgt gaagcccttt tttctctggc ggcgattgaa
    30241 gaagaactgg taccagcgaa agcctgttcc ggtctgcgtc aggtgctgga aaacgtgatg
    30301 ctcgaccgcc cggaatactg gcaaagccac taccacggtg acggcaacgc gcgtcgtctg
    30361 gcgcgtggtt acagctactc agatcgcgtg cgctattact ggccggacag ccagattgat
    30421 gacgctttcg ctcatctggt tcgtaatctg gcggattcac caattccgct accgctcatc
    30481 agccagtatc tgccgctgca gtacgtgaaa gttcgctccg gcgagctgca accgacgcca
    30541 cgggaactca ttataaacca tattcaggac atcctggcgc agtaccacac agcctgtgaa
    30601 ggccaataag caaaacaaag aggaacatgc tatgccaaat attgttttaa gccggattga
    30661 tgaacgcttg attcacggtc aggtcggcgt tcagtgggtc ggatttgcgg gggcaaatct
    30721 ggtgctggta gccaacgatg aggttgccga agatccggta caacaaaacc tgatggaaat
    30781 ggtactggca gaagggatcg ccgtacgttt ctggacgctg caaaaagtta tcgacaacat
    30841 tcatcgcgcc gccgatcgac agaaaatcct gctggtttgt aaaacacccg ccgatttcct
    30901 gacgctggtg aaaggtggcg ttccggtgaa tcgcattaac gttggcaata tgcactacgc
    30961 caatggcaaa caacaaatcg ccaaaacggt ttctgtggat gcgggcgata tcgcagcatt
    31021 taacgacctg aaagccgctg gggtggaatg cttcgttcag ggcgtcccga cagagcctgc
    31081 tgtggacctc tttaaattac tttgagggat tcatcatgga aatcagcctg ttgcaggcat
    31141 ttgcgttggg cattatcgcc tttatcgctg gcctagatat gtttaacggc ttaacccata
    31201 tgcaccgccc ggtggtcctc ggcccgttgg tcgggctggt acttggcgat ctgcataccg
    31261 gaattttaac cggcgatacg ctggaactag tgtggatggg gctggccccg ctggcgggcg
    31321 cacagccgcc taacgtgatt atcggtacta tcgtcggcac ggcgtttgcc attactactg
    31381 gcgtgaaacc cgatgtcgcg gtcggtgtcg ccgtaccttt cgctgtcgca gtacagatgg
    31441 ggattacctt cctgttctcg gtgatgtctg gcgtgatgtc tcgctgcgac cggatggcgg
    31501 agaacgccga tactcgcggc attgaacgcg tgaactatct ggcactgctg gcactcggca
    31561 ctttctattt tctctgcgct ttcctgccta tctacttcgg cgcggaacat gcaaaaacca
    31621 tcattgatgt cctgccgcaa cgattaatcg acggcctcgg cgtcgcgggc ggcatcatgc
    31681 cagcaatcgg ttttgccgtg ctgctgaaaa tcatgatgaa aaacgtctac ataccctact
    31741 tcatcctggg ctttgttgcc gcaacctggc tcaagttacc ggtgctggct attgctgccg
    31801 ccgcgctggc gatggcgctg atcgatctgc tgcgtaaatc tcctgaaccg actcaacctg
    31861 cggcacagaa agaggaattt gaagatggca tctaatcaaa ccaccctgcc gaacgtcagc
    31921 gaaaatgaag aaacactgct gactggcgtc aatgaaaacg tgtatgaaga tcggagcatt
    31981 gccgcggagc tgacgaaaaa agatatcaac cgcgtcgcct ggcgttccat gctgttacaa
    32041 gcgtctttta actacgaacg tatgcaggct tctggctggt tgtacggtct gctgcctgct
    32101 ctgaaaaaga tccacactaa taaacgcgac ctggcgcgcg ccatgaaggg gcatatgggt
    32161 ttcttcaata cccatccgtt tctggtgacg tttgttatcg gcattatcct tgcgatggag
    32221 cgttctaagc aggacgttaa cagtattcag agcaccaaaa ttgccgtcgg tgcgccgctt
    32281 ggcgggattg gcgatgcgat gttctggcta acgctactgc cgatttgtgg cgggataggc
    32341 gcaagcctcg cgctacaagg ctctattctt ggcgcagtcg tctttattgt gctgttcaac
    32401 gtggtacact tggggctgcg ttttggtctg gcgcattatg cttaccgcat gggcgtggcg
    32461 gcgattcccc ttattaaagc gaataccaaa aaagtcggcc atgctgcgtc aatcgttgga
    32521 atgacggtaa tcggcgcact ggtggcaacc tatgttcgtt taagcaccac gctggaaatc
    32581 acggcgggcg acgcagtagt taagttacag gctgatgtta tcgacaaact gatgcctgcc
    32641 ttcttaccgc tggtctacac cctgaccatg ttctggctgg tacgccgcgg ctagagtccg
    32701 ctgcgcctga ttgcggttac cgtggttcta gggatcgtcg gtaaattctg ccatttcctt
    32761 taaatacaat gaggtattag atgttaagta ttattttgac agggcatggc gggtttgcca
    32821 gtggcatgga aaaagcaatg aagcaaatcc tcggcgatca atcgcagttt attgccatcg
    32881 attttcccga aacctcaagc gccgcgctgc ttacatcgca gcttgaagag gccatcgccc
    32941 aactggattg tgaagacggc attgtttttc tgacggattt gctgggcggc acgccgtttc
    33001 gcgtagcttc aacgctggcg atgcaaaaac cgggctgtga agtgattacc ggcaccaatt
    33061 tgcaactgct actggagatg gtgctggagc gcgaagggtt aagcggtgaa gaattccgtg
    33121 tacaggcgct ggagtgtgga caccgagggc tgaccagtct ggtggacgag ttaggccgct
    33181 gccatgaaga atgtccggtc gaggaaggaa tatgacacac gttctgcgcg ccagaaggct
    33241 gctgactgaa gagggatggt tcgatgacca tcagttgcgt gttgctgacg gtgtcatcgc
    33301 agcaatcgaa ccgattccag tgggcgtgac tgaacgcgat gcggaacggc tctgccccgc
    33361 ttacatcgac acccatgtac acggtggtgc gggcgttgat gtaatggatg acgcgccgga
    33421 tgtactcgac aagctggcaa tgcacaaggc acgcgaaggt gtcggcagtt ggttaccgac
    33481 caccgtaacc gcgccgctta ataccattca tgcggcgctg aaacgtattg ctcaacgttg
    33541 ccaacgcggc ggacctggtg cgcaagtgct ggggag
//
LOCUS       DAJTUP010000035        33331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTUP010000035 DAJTUP010000000
VERSION     DAJTUP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.035"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1254
                     /gene="waaA"
                     /locus_tag="OO468_001733"
     CDS             <1..1254
                     /gene="waaA"
                     /locus_tag="OO468_001733"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR6258879.1"
                     /translation="LLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRHPLKPGGIM
                     LHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQHVYLPYDL
                     PDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSAAGYAKLGK
                     FVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLAAKAVTLRR
                     QWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPDAINLVRQA
                     GLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGHNPLEAAAH
                     AIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRSFYGRHAVE
                     VLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1262..1741
                     /gene="coaD"
                     /locus_tag="OO468_001734"
     CDS             1262..1741
                     /gene="coaD"
                     /locus_tag="OO468_001734"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR6258880.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1780..2589)
                     /gene="mutM"
                     /locus_tag="OO468_001735"
     CDS             complement(1780..2589)
                     /gene="mutM"
                     /locus_tag="OO468_001735"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR6258881.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2687..2854)
                     /gene="rpmG"
                     /locus_tag="OO468_001736"
     CDS             complement(2687..2854)
                     /gene="rpmG"
                     /locus_tag="OO468_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR6258882.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2875..3111)
                     /gene="rpmB"
                     /locus_tag="OO468_001737"
     CDS             complement(2875..3111)
                     /gene="rpmB"
                     /locus_tag="OO468_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR6258883.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3328..3996)
                     /gene="radC"
                     /locus_tag="OO468_001738"
     CDS             complement(3328..3996)
                     /gene="radC"
                     /locus_tag="OO468_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR6258884.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4168..5388
                     /gene="coaBC"
                     /locus_tag="OO468_001739"
     CDS             4168..5388
                     /gene="coaBC"
                     /locus_tag="OO468_001739"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR6258885.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5366..5824
                     /gene="dut"
                     /locus_tag="OO468_001740"
     CDS             5366..5824
                     /gene="dut"
                     /locus_tag="OO468_001740"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR6258886.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            5931..6527
                     /gene="slmA"
                     /locus_tag="OO468_001741"
     CDS             5931..6527
                     /gene="slmA"
                     /locus_tag="OO468_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR6258887.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6564..7205)
                     /gene="pyrE"
                     /locus_tag="OO468_001742"
     CDS             complement(6564..7205)
                     /gene="pyrE"
                     /locus_tag="OO468_001742"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR6258888.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7271..7987)
                     /gene="rph"
                     /locus_tag="OO468_001743"
     CDS             complement(7271..7987)
                     /gene="rph"
                     /locus_tag="OO468_001743"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR6258889.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8114..8977
                     /locus_tag="OO468_001744"
     CDS             8114..8977
                     /locus_tag="OO468_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR6258890.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9198..10022
                     /gene="dinD"
                     /locus_tag="OO468_001745"
     CDS             9198..10022
                     /gene="dinD"
                     /locus_tag="OO468_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR6258891.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10313..10930
                     /locus_tag="OO468_001746"
     CDS             10313..10930
                     /locus_tag="OO468_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR6258892.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10927..12609)
                     /gene="ligB"
                     /locus_tag="OO468_001747"
     CDS             complement(10927..12609)
                     /gene="ligB"
                     /locus_tag="OO468_001747"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR6258893.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12867..13490
                     /gene="gmk"
                     /locus_tag="OO468_001748"
     CDS             12867..13490
                     /gene="gmk"
                     /locus_tag="OO468_001748"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR6258894.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13545..13820
                     /gene="rpoZ"
                     /locus_tag="OO468_001749"
     CDS             13545..13820
                     /gene="rpoZ"
                     /locus_tag="OO468_001749"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR6258895.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13839..15947
                     /gene="spoT"
                     /locus_tag="OO468_001750"
     CDS             13839..15947
                     /gene="spoT"
                     /locus_tag="OO468_001750"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR6258896.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            15954..16643
                     /gene="trmH"
                     /locus_tag="OO468_001751"
     CDS             15954..16643
                     /gene="trmH"
                     /locus_tag="OO468_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR6258897.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16649..18730
                     /gene="recG"
                     /locus_tag="OO468_001752"
     CDS             16649..18730
                     /gene="recG"
                     /locus_tag="OO468_001752"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR6258898.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18764..19969)
                     /gene="gltS"
                     /locus_tag="OO468_001753"
     CDS             complement(18764..19969)
                     /gene="gltS"
                     /locus_tag="OO468_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR6258899.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20249..21640
                     /gene="xanP"
                     /locus_tag="OO468_001754"
     CDS             20249..21640
                     /gene="xanP"
                     /locus_tag="OO468_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR6258900.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21761..23470
                     /locus_tag="OO468_001755"
     CDS             21761..23470
                     /locus_tag="OO468_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6258901.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23523..25841)
                     /gene="yicI"
                     /locus_tag="OO468_001756"
     CDS             complement(23523..25841)
                     /gene="yicI"
                     /locus_tag="OO468_001756"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR6258902.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25851..27234)
                     /locus_tag="OO468_001757"
                     /pseudo
     CDS             complement(25851..27234)
                     /locus_tag="OO468_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27527..27621
                     /locus_tag="OO468_001758"
     tRNA            27527..27621
                     /locus_tag="OO468_001758"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27561..27563,aa:Sec,seq:tca)
     gene            27893..29104
                     /locus_tag="OO468_001759"
     CDS             27893..29104
                     /locus_tag="OO468_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6258903.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29516..30559
                     /locus_tag="OO468_001760"
     CDS             29516..30559
                     /locus_tag="OO468_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR6258904.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31074..31502
                     /locus_tag="OO468_001761"
                     /pseudo
     CDS             31074..31502
                     /locus_tag="OO468_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31532..32683)
                     /locus_tag="OO468_001762"
                     /pseudo
     CDS             complement(31532..32683)
                     /locus_tag="OO468_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         8334 a         8767 c         8228 g         8002 t
ORIGIN      
        1 cttctctacc ttattcagcc gctgatctgg atacggctct gggtgcgcgg acgtaaggct
       61 ccggcctatc gaaaacgctg gggtgaacgt tacggttttt accgccatcc gctaaaacca
      121 ggcggcatta tgctgcactc cgtctccgtc ggtgaaactc tggcggcgat cccgttggtg
      181 cgcgcgctgc gtcatcgtta tcctgattta ccgattaccg ttacaaccat gacgccaacc
      241 ggttcggagc gcgtacaatc ggctttcggg aaggatgttc agcacgttta tctgccgtat
      301 gacctgcccg atgcgctcaa ccgtttcctg aataaagtcg accccaaact ggtgttgatt
      361 atggaaaccg aactatggcc taacctgatt gcggcgctac ataaacgtaa aattccgctg
      421 gtgatcgcta acgcgcgact ctctgcccgc tcggccgcag gttatgccaa actgggtaaa
      481 ttcgtccgcc gcttgctgcg tcgtattacg ctgattgctg cgcaaaatga agaagatggt
      541 gcacgttttg tggcgctggg cgcaaaaaat aaccaggtaa ccgttaccgg tagcctgaaa
      601 ttcgatattt ctgtaactcc gcagttggct gctaaagccg tgacgctacg ccgccaatgg
      661 gcaccacacc gcccggtatg gattgccacc agcactcacg aaggtgaaga gagcgtggtc
      721 attgccgcac atcaggcatt gttacagcaa ttcccgaatt tattgctcat cctggtaccc
      781 cgtcatccag aacgcttccc ggatgcgatt aaccttgtcc gccaggctgg actaagctat
      841 atcacacgct cttcagggga agtcccctcc accagcacgc aggttgtggt tggcgatacg
      901 atgggcgagt tgatgttact gtacggcatt gccgatctcg cctttgttgg cggttcactg
      961 gttgaacgtg gtgggcataa tccgctggaa gctgccgcac acgctattcc ggtattgatg
     1021 gggccgcata cttttaactt taaagacatt tgcgcgcggc tggagcaggc aagcgggctt
     1081 attaccgtta ccgatgccac tacgcttgca aaagaggttt cctctttact caccgacgcc
     1141 gtttaccgta gtttctatgg ccgtcatgcc gttgaagtac tgtatcaaaa ccagggcgcg
     1201 ctacagcgtc tgcttcaact gctggaacct tacctgccac cgaaaacgca ttgaggttgt
     1261 tatgcaaaaa cgggcgattt atccgggtac tttcgatccc attaccaatg gtcatatcga
     1321 tatcgtgacg cgcgccacgc agatgttcga tcacgttatt ctggcgattg ccgccagccc
     1381 cagtaaaaaa ccgatgttta ccctggaaga gcgtgtggca ctggcacagc aggcaaccgc
     1441 gcatctgggg aacgtggaag tggtcgggtt tagtgatttg atggcgaact tcgcccgtaa
     1501 tcaacacgct acggtgctga ttcgtggcct gcgtgcggtg gcggattttg aatatgaaat
     1561 gcagctggcg catatgaatc gccacttaat gccggaactg gaaagtgtgt ttctgatgcc
     1621 gtcgaaagag tggtcgttta tctcttcatc gttggtgaaa gaggtggcgc gccatcaggg
     1681 cgatgtcacc catttcctgc cggagaatgt ccatcaggcg ctgatggcaa aattagcgta
     1741 gcgtttatgc cggatggtat gccatccggc tcgcatgaat tacttctggc actgccgaca
     1801 ataaaacgtt gcccgctgcg catgtttagt cgccacaatc ggcgtaccgc acacccgaca
     1861 cggctcacct tttcgcccgt aaacctgcaa ttcctgcgcg aaatagcccg gtttaccatc
     1921 actttgcaga aaatctttca gcgttgtacc accctgctca atcgaacgca gcaacaccgc
     1981 tttaatcacc cgagctaaca attcacactc tgccagcgac agtgatgacg ccagccgatc
     2041 cggatggatc cccgccgcaa acagtgattc gctggcatag atattcccta cccctaccac
     2101 cagcttgtta tccatcagcc acggtttaat cgccgttttt ttcttcgcgc acttctgatg
     2161 cagatactca ccattgaaat cgtcgctaag cggctccggt ccaagatggg ccagcacatt
     2221 atgcccttcc agctctttgg tccacagcca ggcaccaaag cggcgcggat cggtgtagcg
     2281 cagcacttta ccgttgctca tcaccaaatc cacatggtca tgcttttcag ggggaagttc
     2341 ttctggaagg atgcgcaggc tgccagacat ccccaaatga atgataatcc agccctcagg
     2401 cagctccagc agcagatatt tagcccgccg ctgcacgcta agcactggtt gatcgcttaa
     2461 tcggtagatc tcttctgaaa ccggccagcg caagcgtccg ttgcgcacca ccgcatgaag
     2521 aatggttgca ccaacgagat gcggttctat gccgcgacgg ctggtttcaa cttcgggtaa
     2581 ttcaggcata gcatctccag gaatgaacag atgcaatcaa tatgggggca agcagataac
     2641 aaaaaacccc gccggagcga ggtttttcgt tacatcaaag cgagaattat ttgattttcg
     2701 cttctttgta gatcacgtgc tgacgaacaa ctggatcgaa ttttttcagt tccagttttt
     2761 ccggcttagt acgtttgttc ttcgtagtgg tatagaagtg accagtacca gcagaagaaa
     2821 ccagcttgat tttctcacga atacctttag ccatgattta tttcctctaa gtacttagta
     2881 cttttcgcca cgggcacgca gttcagccag aactgtatcg atgccttttt tatcgattac
     2941 acgcatacct ttagcagata cgcgcagggt gacaaaacgc ttctcgctct caacccagaa
     3001 acggtgagag tgcaggttcg gcaggaaacg gcgtttagtc gcgttcagtg cgtgggaacg
     3061 gttgttaccg gtcaccggac gcttgccagt aacttggcag actcgggaca tgtctattct
     3121 ccaaaaatca aattagctcg agcttcgtat ggggtattgg cgcctcgtca ggctttacag
     3181 cccggtcatc gcagttctat gtgaactctc gattgccagg cccaaatgcc aaacccgaga
     3241 ttctcaaagg tggcgtagta tacgctgact cagcgatgtg ctcaagtccc gaacagacaa
     3301 agatcccgaa ggatcgcgca tagcgggtta aatccagccg cgttcggcaa aagaaacata
     3361 ctctccacgc ccaatcacga tatggtcgag cacgcgtaaa tccatgaact gacaactctt
     3421 tattatccgt tcagtaatga gtttatccgc tttactgggt tcagcacaac ccgaagggtg
     3481 attatgtgca aggatcagcg ccgaggcgtt tatttttatc gcttcgcgga taatttcccg
     3541 aggatggact tcaacatggt ttagcgtgcc ggaaaaaaga cggctatgcg ttataacccg
     3601 gtgttgtgag tcgagaaaga tcaccataaa gatctcccgc tcctcacccg tgagctggct
     3661 ttgtaaaaat tcccgcgtca tctccgggct gagtaaaggg ctttcttcac gcatccgcac
     3721 gttgtagtaa cgtcgcgcca gttcagcaat cccctttaac tgggcaaatt tcgctacgcc
     3781 aattccatga acaccactaa attgttcata ttcagaggtt aacaagccat aaagagagcc
     3841 gaaattctcc agcatctctt ttgccagggt taatacatct ttaccgcgcg ttccggtacg
     3901 cagaaatagc gccagcagct cgacatccgt taaggcgcta ataccaaact tcagcatttt
     3961 ttcgcgcggc atcaacagct gtgcattgtt tttcaccttc acctcctttg tggtgctcgc
     4021 atcctgtcat agccgttccg ttaaatcgac ggccagtttt caatcctgga aagagcctcg
     4081 caaagtgaat ctcaggtgaa ggcacgacaa caaaagtatt gtgataaaat cgccaacttc
     4141 tggtgtcacc caacaggaaa aatcatcatg agcctggccg gtaaaaaaat cgttctcggc
     4201 gttagcggcg gtattgctgc ctataaaacc cctgaactgg tgcgtcgttt gcgcgatcgc
     4261 ggggccgacg tccgcgtagc catgaccgaa gcggcaaaag cctttatcac cccacttagc
     4321 ttgcaggcgg tttctggtta tcccgtttcc gacagtctgc ttgacccggc agccgaagcc
     4381 gctatgggcc atattgagct gggtaaatgg gctgatttag tgattctcgc ccctgccacg
     4441 gcagatttga ttgcccgtgt tgctgccgga atggcgaatg acctggtatc gacgatttgt
     4501 ctggctacac ctgcgcctgt agccgtgctc cccgccatga accagcagat gtaccgtgcc
     4561 gctgccacgc agcataattt agaggtgctt gcttcccgtg gtttgctcat ctgggggcca
     4621 gacagtggca gtcaggcttg tggtgatatc ggtcctggac gaatgctcga tccgttaacc
     4681 attgtggata tggcggtggc gcatttttcg cccgtcaacg acctgaaaca tctgaacatt
     4741 atgattaccg ccggtccgac gcgtgaaccg ctcgatccgg tgcgttatat ctctaatcac
     4801 agctccggca agatgggttt tgctatcgcc gccgctgctg cccgtcgtgg cgcgaacgtc
     4861 acgctggtat caggtccagt ttcactaccg acgccaccgt ttgttaaacg tgttgatgtg
     4921 atgaccgcgc tggaaatgga agccgccgtg aatgcttctg tacagcagca aaatattttt
     4981 atcggttgcg ccgccgtggc ggattatcgc gcagctaccg tggccccaga aaaaatcaaa
     5041 aagcaggcca cgcagggtga tgaattaaca ataaaaatgg ttaaaaaccc cgatatcgtc
     5101 gcaggcgttg ccgcactaaa agaccatcga ccctacgtcg ttggatttgc cgccgaaaca
     5161 aataatgtgg aagaatacgc ccggcaaaaa cgtatccgta aaaaccttga tctgatctgc
     5221 gcgaacgatg tttcccagcc aactcaagga tttaacagcg acaacaacgc attacacctt
     5281 ttctggcagg acggagataa agtcttaccg cttgagcgca aagagctcct tggccaatta
     5341 ttactcgacg agatcgtgac ccgttatgat gaaaaaaatc gacgttaaga ttctggaccc
     5401 gcgcgttagg aaggaatttc cgctcccgac ttatgccacc tctggctctg ccggacttga
     5461 cctgcgtgcc tgtctcgacg acgccgtaga actggctccg ggtgacacta cgctggttcc
     5521 gaccgggctg gcgattcata ttgccgatcc ttcactggcg gcaatgatgc tgccgcgctc
     5581 cggattggga cataagcacg gtatcgtgct tggtaacctg gtaggattga tcgattccga
     5641 ctatcagggc cagttgatga tttccgtgtg gaaccgtggt caggacagct tcaccattca
     5701 acctggcgaa cgcatcgccc agatgatttt tgttccggta gtacaggctg aatttaatct
     5761 ggtggaagat ttcgacgcca ccgaccgcgg tgaaggcggc tttggtcact ctggtcgtca
     5821 gtaacacata cgcatccgaa taacgtcata acatagccgc aaacatttcg tttgcggtca
     5881 tagcgtgggt gccgcctggc aagtgcttat tttcaggggt attttgtaac atggcagaaa
     5941 aacaaactgc gaaaaggaac cgtcgcgagg aaatacttca gtctctggcg ctgatgctgg
     6001 aatccagcga tggaagccaa cgtatcacga cggcaaaact ggccgcctct gtcggcgttt
     6061 ccgaagcggc actgtatcgc cacttcccca gtaagacccg catgttcgat agcctgattg
     6121 agtttatcga agatagcctg attactcgca tcaacctgat tctgaaagat gagaaagaca
     6181 ccacagcgcg cctgcgtctg attgtgttgc tgcttctcgg ttttggtgag cgtaatcctg
     6241 gcctgacccg catcctcact ggtcatgcgc taatgtttga acaggatcgc ctgcaagggc
     6301 gcatcaacca gctgttcgag cgtattgaag cgcagctgcg ccaggtattg cgtgaaaaga
     6361 gaatgcgtga gggtgaaggt tacaccaccg atgaaaccct gctggcaagc cagatcctgg
     6421 ccttctgtga aggtatgctg tcacgttttg tccgcagcga atttaaatac cgcccgacgg
     6481 atgattttga cgcccgctgg ccgctaattg ccgcgcagtt gcagtaatat gacgccggat
     6541 gacttttcat ccggcgagtt tctttaaacg ccaaactctt cgcgataggc cttaaccgcc
     6601 gccagatgtt ccgccatttc cggcttctct tccagatagg caatcagatc tttcagggtg
     6661 atgatagaga tcactttgca gttgtaatca cgctcaactt cctgaatcgc cgaaatctcg
     6721 ccgcgcccgc gttcctgacg atcgagcgaa atcaacacgc cagcaagcgt cgcgccattg
     6781 gcctgaataa tctccatcga ctcgcgaatc gccgttccgg cggtgatcac atcatctacc
     6841 agcattacgc gtccttgtaa cgcgctacca accagattgc cgccttcacc gtggtctttt
     6901 gcttctttgc ggttaaagca gtacggcagg tccaggtcgt gatgctccgc cagtgccaca
     6961 gcggttgtgg tggcaatcgg gatccctttg taagcagggc caaacagcag atcgaactca
     7021 atgccggaat ccaccaacgc ttcagcgtaa aaacggccta acagtgccag atcgcgcccg
     7081 gtattaaaca gcccggcgtt gaagaaatag gggcttttgc gcccggattt cagcgtaaac
     7141 tcgccaaact ttaacacctg cttgctaagc gcaaattcaa taaactggcg ctgatatgat
     7201 ttcatgcctt cgctcctcat cttacttttc tacagacaaa aaaaaggcga ctcatcagtc
     7261 gccttaaaaa tcagtttgcc agcgccgcct tctgcgtcgc tacaatggat tcgattcccc
     7321 ctcgggccag cgccaacaag gtgagtagct cttcatgggt gaacggctcg ccttctgccg
     7381 tcccctgcac ttcaatgatg cgcccgtctt cggtcatcac tacgttcatg tcggtctctg
     7441 cggctgagtc ttcaacgtat tccagatcgc aaattgcttc accgtttaca ataccgacag
     7501 aaactgcggc taccatccct ttcatcgggt tggttttcag cttgccgttt tccaccagct
     7561 tctgtagcgc atctgccagc gccacgcagg cacccgtaat cgacgcggta cgagtgccac
     7621 catcagcctg aagcacgtcg cagtccagcg taatggtgaa ctcacccagc gctttcaaat
     7681 ctactgccgc gcgaagagca cgggcgatca gacgctggat ttccattgtg cgtccaccct
     7741 gcttaccttt cgccgcttca cgagcgttac gggtgtgggt agaacgtggc agcatgccgt
     7801 actctgcggt gatccagccc tggccctgac ctttcaggaa gcgcggcacg ccttcttcaa
     7861 tagaggcggt acacaatact ttggtatcgc caaattcgac cagcaccgag ccttctgcat
     7921 gttttgtata gttacgagtc agggtaacgg gacgcacctg attattgcta cggcctgctg
     7981 gacgcatatt gaaatctccg gcttgaaaca aatgtggctg cgcattatac ggacttccgg
     8041 cggttattcc tatcctgaca aggcatcgat ggctataatc cttccacctc tccttttata
     8101 aacaggaacg tctatgatcc gcagtatgac cgcctacgcc cggcgtgaaa tcaagggtga
     8161 atgggggagc gcaacctggg aaatgcgctc ggtaaaccag cgttatctgg aaacttactt
     8221 tcgtctgccg gagcagttcc gtagccttga acctgtcgtt cgcgagcgta ttcgttctcg
     8281 cctgacgcgc ggtaaagtgg aatgtaccct gcgctatgag ccagatgtta gcgcacaagg
     8341 tgagctgatc ctcaatgaaa aactggctaa acagctggta actgccgcga actgggtaaa
     8401 aatgaagagt gacgaagggg aaatcaaccc ggttgatatt ctacgctggc cgggcgtgat
     8461 ggcagcccag gagcaggatc tcgacgcgat tgccgctgaa attctcgcgg cgttggatgg
     8521 tacgctggac gactttatcg tcgcgcgcga aaccgaaggt caggcactga aagcattgat
     8581 cgagcagcgt ctggaaggcg tcaccgccga agtggtcaaa gtccgcgccc atatgccgga
     8641 aattctgcaa tggcagcgtg agcgtctggt cgcgaagctg gaagatgctc aggttcagtt
     8701 ggaaaacaac cgtctggagc aggagttggt tctgctggca caacgaattg acgttgccga
     8761 agagctggac cgcctcgaag cgcatgttaa agagacgtac aacattctga agaaaaaaga
     8821 agcggttggt cgtcgtctgg actttatgat gcaggagttc aaccgtgagt cgaacactct
     8881 tgcgtcgaaa tctatcaatg ccgaagtgac aaactccgcc atcgagctga aagtgttgat
     8941 tgagcagatg cgcgagcaga ttcagaatat tgagtaactc gtaacaccaa ttctgacttt
     9001 tcagccttca caaatgccgc ccactcaaac agagcggcat ttttcttccc cgcaacttca
     9061 attctgtttt gcgtgcctgc tccagatttt gcgatgtttt tttgcccagc acactgagaa
     9121 cgtgagatac tcacaactgt atataaatac agttacagat ttactttctt tgcaatcgat
     9181 atcacatgga gtgggcaatg aacgaacatc atcaaccttt tgaagagata aaactgatta
     9241 atgcaaacgg agcagaacaa tggtcagcaa gacaacttgg gaaactactg ggttattcag
     9301 agtatcgtca ctttatacct gtattaacgc gcgccaaaga ggcctgtgag aacagtggtc
     9361 acacaattga tgaccatttc gaggagatcc tcgatatggt caaaattggc tcaaatgcca
     9421 aaagagcatt aaaagacatc gtactctccc gctatgcctg ttacctggta gtacaaaacg
     9481 gcgaccccgc gaaaccggtc attgcggcag ggcagactta ttttactatc cagacccgac
     9541 ggcaggagct tgctgacgat gaagcattta agcaacttcg tgaagacgaa aagcgtctgt
     9601 ttctgagaaa tgagttgaaa gaacataaca aacaattggt tgaggcagca cagcaagctg
     9661 gggtagcgac agctactgat ttcgccatat ttcagaatca tggttaccag gggctgtatg
     9721 gtggattaga tcagaaagcc atccaccaac tgaaggggct gaaaaagagt cagaagatcc
     9781 tggatcatat gggttcaaca gaactggcgg ctaatctctt tcgagctacc caaacagaag
     9841 aaaaactcaa gcgggatagc gtgaattcaa aacagcaagc taataccaca cactttgacg
     9901 tgggtagcaa ggtgaggcaa accattcagg aacttggcgg aaccatacct gaggagttgc
     9961 cgaccccgca ggtcagcatc aagcagttgg aaaacagtgt aaaaattaca gagaagaaat
    10021 agcagaaacg aaaaactctt tttgttgatt gagacacccg atgcgtaagc caaggtccag
    10081 gtgcagtgaa cacaatggct aaacattgca cctttctttt cccccctcag ttttaaccta
    10141 ttttttctta tgcattttct cagacaagaa gtcagaagaa tgcatctacg ctacagaaaa
    10201 tagcgatttc acataactaa aagttatcaa tttcctctca ccttaaaaaa tatcaatcgt
    10261 gacaatgcgc acaaatcgct accctgccag acagattttt agggagagaa ccatgctgtt
    10321 acacattttg tatttggttg gcattactgc cgaagccatg acgggggcgc tggcggccgg
    10381 gcgacggcgc atggatacat ttggcgtaat tattattgca accgccaccg caattggcgg
    10441 agggtcagtg cgcgatattc tgctgggcca ctatccgctc ggctgggtca aacacccgga
    10501 atatgtgatt atcgtcgcta ccgccgcagt actcaccact atcgttgctc ctgtaatgcc
    10561 ttacctgcgc aaagtgtttc tggtgctcga tgcgctcgga ctggtggtct tttctatcat
    10621 cggcgcacag gttgcactgg atatggggca cggcccaatt attgccgttg tcgcggcggt
    10681 aaccaccggt gtatttggcg gcgttttgcg cgatatgttc tgtaaacgca ttccgctggt
    10741 ctttcaaaaa gagttgtatg ccggtgtctc atttgcctcc gccgtgctgt acatcgcgct
    10801 gcaacactat gtttctaacc atgatgtggt gattatctcc accctggtat tcggcttttt
    10861 tgctcgttta ctggcgctac gtcttaagct gggattaccg gtcttttact acagccacga
    10921 aggtcactaa ggttcaaaac ctgtgatctg ctgggcagcc agccaactgc ccagcttctt
    10981 gatttgcgca ttttccttcc attcaataac ctgtctggcg cgcccagatc cagtccccgg
    11041 cagctgctgc cagaactgct ccgtgctaaa taaaagttgc gaccaggacc gttcatcact
    11101 ggcattaagc gccgcccggg ttagcggtat tcccattgcc atcacccagc gagtaaaagg
    11161 ctggttacga gccagattaa actgatgcca taactgcgca cttttacttt tcgcgatccc
    11221 cggcgtgttc tgtaattgct ctggcgttaa taaaagccag gaaaagatat gctcaaagcg
    11281 atgagtctga tgcagcgcgc gccaaccggc ctcaccaatg ccatccagcc ccagaacctg
    11341 ttttgacccc agccagacta agcgtgaaat gaactgttcc tgacaaacat cagaagcaaa
    11401 gtagcaggtc aacgggttaa agcggttttc tggtggtgtc ggttttgtac gttctgcacc
    11461 gcgccagacc acatcatcaa tgcgaggaat accctgaccg gcaaggctga cgaggatctg
    11521 atcaccaggc gcaatatccc actcctgcca gcgcctgacg gaaccaatat tcacccgctg
    11581 gactttttta tcatccagca tgacaggtgc gagtgacgca accaccgata ttttaccgct
    11641 cttacccacc gcaaactgaa tcgccttcac ttcggcaacc tgagctacag gttgatattt
    11701 ccaggccacc agccactccg cctggcccgg tagccaatga cgggattcgg gttccttagc
    11761 ccctcgcaca accacgccat cggtgacgaa gggtaatttc gctttccacc actcattgcg
    11821 tacgcgcgca acctcatcag catttttaac cgcacgggta tacctctgcg ttagagtaaa
    11881 acctgcggta gccagctcct ttaaacgatc ggtcattaac tgcggtccat ccggccatgc
    11941 ccagacaaaa acagccagtg agtttagcgt gtcgctattc ccctggcgca tcatcaggcc
    12001 agcaactttt gcgcgggcat ttattccccc catttgttgt tggatatgcc cctcgcgctg
    12061 gagaaatatt tccccctgaa gcgtactgtt ggctaaaggc ccgctaacgg tttgcagcac
    12121 agcggaaatt aagctaactt tctgcgtcca gtcctcgcct ttcaggccat taccgcgact
    12181 gattgctttg ttcagtttcc cgtcccgata aaccagggtt accgcaacgc catcaacttt
    12241 tggctgcacc caaagatcgc tacgttctcg catccacaga cttaatgcgt ttttatccgc
    12301 cattttgcgt acacccgtat gggcaaccgg atgcataacc gcaccattta aaggcggcat
    12361 catcacgtcg cggggctcgc tcacaaagca gcgttgccac tgcgtaagac gggcgcttaa
    12421 ctgatcgtaa acaccgtctt ccacctcact ttttccttcc ttccagtagt catcgtccca
    12481 ctgttttatt tgctgttgca ggcgggaaat ttcttcctgt gctctggctg gcgaccaggc
    12541 cggacagacc gcccacacag atgattgcca gcacaagata cttattaata tcgccatcca
    12601 tactttcatc ttcacctccg ctgtagtcag gctggcagat atacaacgtg gtgaaggcaa
    12661 agccgagtgg caaaaacgga gtctgcgagg acgcttcctg agaatcgtct ttattgcagt
    12721 aaatcacagg caaatgcgga aacagctacg caaaacgcaa caactttgcg caaaaagtgt
    12781 gagcaagggc tacgtcacat ggccgcgccg tgtataataa gctcgtatgt aggctttatt
    12841 tcgctaatca catacgaaag atactcatgg ctcaaggcac gctttatatt gtttctgccc
    12901 ccagtggcgc gggtaaatcc agcctgattc aggctttatt aaaaacccaa ccgttgtatg
    12961 acacccaggt ttctgtttca cacaccacac gccaaccgcg tcctggtgaa gtccacggtg
    13021 aacattattt ctttgttaat catgatgaat ttaaagaaat gattagcaga gatgcgttcc
    13081 tcgaacacgc agaagttttt ggtaattact atggcacttc gcgtgaggcc attgagcaag
    13141 tactggcgac cggtgtcgat gtttttctcg atatcgactg gcagggcgcg cagcaaattc
    13201 gccagaagat gccgcacgcg cggagtatct ttattttacc gccgtccaaa attgaactgg
    13261 accgccgtct acgcggtcgc ggtcaggaca gcgaagaggt cattgcaaag cgtatggcgc
    13321 aagctgttgc agaaatgagc cattacgccg aatatgatta tctgattgtg aatgatgact
    13381 tcgataccgc gttgaccgat ttgaagacca ttattcgcgc cgaacgtctg cgcatgagcc
    13441 gccaaaagca gcgtcatgac gctttaatca gcaaattgtt ggcagactga acctgatttc
    13501 agtatcatgc ccagtcattt cttcacctgt ggagcttttt aagtatggca cgcgtaactg
    13561 ttcaggacgc tgtagagaaa attggtaacc gttttgacct ggtactggtc gccgcgcgtc
    13621 gcgctcgtca gatgcaggta ggcggaaagg atccgctggt accggaagaa aacgataaaa
    13681 ccactgtaat cgcgctgcgc gaaatcgaag aaggtctgat caacaaccag atcctcgacg
    13741 ttcgcgaacg ccaggaacag caagagctgg aagccgctga attacaagcc gttaccgcta
    13801 ttgctgaagg tcgtcgttaa tcacaaagcg ggtcgccctt gtatctgttt gaaagcctga
    13861 atcaactgat tcaaacctac ctgccggaag accaaatcaa gcgtctgcgg caggcgtatc
    13921 tcgttgcacg tgatgctcac gaggggcaaa cacgttcaag cggtgaaccc tatatcacgc
    13981 acccggtagc ggttgcctgc attctggccg agatgaaact cgactatgaa acgctgatgg
    14041 cggcgctgct gcatgacgtg attgaagata ctcccgccac ctaccaggat atggaacagc
    14101 tttttggtaa aagcgtcgcc gagctggtag agggggtgtc gaaacttgat aaactcaagt
    14161 tccgcgataa gaaagaggcg caggccgaaa actttcgcaa gatgattatg gcgatggtgc
    14221 aggatatccg cgtcatcctc atcaaacttg ccgaccgtac ccacaacatg cgcacgctgg
    14281 gctcacttcg cccggacaaa cgtcgccgca tcgcccgtga aactctcgaa atttacagcc
    14341 cgctggcgca ccgtttaggt atccaccaca ttaaaaccga actcgaagag ctgggttttg
    14401 aggcgctgta tcccaaccgt tatcgcgtaa tcaaagaagt ggtgaaagcc gcgcgcggca
    14461 accgtaaaga gatgatccaa aaaatcctct cggaaatcga agggcgtttg caggaagcgg
    14521 gaattccctg ccgcgtcagt ggtcgcgaga agcatcttta ttcgatttac tgcaaaatgg
    14581 tgctcaaaga gcagcgtttt cactcaatca tggacatcta cgctttccgc gtgatcgtca
    14641 atgattccga cacctgttat cgcgtgctcg ggcaaatgca tagcctgtac aagccgcgtc
    14701 cgggtcgcgt gaaagactac atcgccattc caaaagcgaa cggctatcag tctttgcaca
    14761 cctcgatgat cggcccgcac ggtgtgccgg ttgaggtcca gatccgtacc gaagatatgg
    14821 atcagatggc ggagatgggt gttgccgcgc actgggctta taaagagcac ggcgaaacca
    14881 gcaccaccgc gcaaatccgc gcccagcgct ggatgcaaag cctgctggag ctgcaacaga
    14941 gcgccggtag ttcgtttgaa tttatcgaga gcgttaaatc cgatctcttc ccggatgaga
    15001 tttacgtttt cacaccggaa gggcgaattg tcgagctgcc tgccggtgca acgcccgtcg
    15061 acttcgctta tgcagtgcat accgatatcg gtcatgcctg cgtgggcgca cgcgttgacc
    15121 gccagcctta cccgctgtcg cagccgctta ccagcggtca aaccgttgaa atcattaccg
    15181 caccgggcgc tcgcccgaat gccgcgtggc tgaactttgt cgttagctca aaagcgcgcg
    15241 ccaaaattcg tcagttgctg aaaaacctca agcgtgatga ttctgtaagc ctgggccgtc
    15301 gtctgctcaa ccatgctttg ggtggtagcc gtaagctgaa tgaaatcccg caggaaaata
    15361 ttcagcgcga gctggatcgc atgaagctgg caacgcttga cgatctgctg gcagaaatcg
    15421 gacttggtaa cgcaatgagc gtggtggtcg cgaaaaatct gcaacatggg gacgcctcca
    15481 ttccaccggc aacccaaagc cacggacatc tgcccattaa aggtgccgat ggcgtactga
    15541 tcacctttgc gaaatgctgc cgccctattc ctggcgaccc gattatcgcc cacgtcagcc
    15601 ccggtaaagg tctggtgatc caccatgaat cctgccgtaa tatccgtggc taccagaaag
    15661 agccagagaa gtttatggct gtggaatggg ataaagagac ggcgcaggag ttcatcaccg
    15721 aaatcaaggt ggagatgttc aatcatcagg gcgcgctggc aaacctgacg gcggcaatta
    15781 acaccacgac ttcgaatatt caaagtttga atacggaaga gaaagatggt cgcgtctaca
    15841 gcgcctttat tcgtctgacc gctcgtgacc gtgtgcatct ggcgaatatc atgcgcaaaa
    15901 tccgcgtgat gccagacgtg attaaagtca cccgaaaccg aaattaatgt tttatgaacc
    15961 caacacgtta tgcacgcatc tgcgaaatgc tcgccaggcg gcagcctgat ctgaccgtct
    16021 gcatggagca ggtccacaaa cctcataacg tttctgcgat tattcgtacc gcagatgccg
    16081 ttggcgtaca tgaagttcac gccgtctggc ctggtagccg catgcgcacc atggcttcgg
    16141 cagcggcggg tagtaacagc tgggtacagg tgaaaacaca ccgcaccatt ggcgatgccg
    16201 tcgctcatct caaaggccag ggcatgcaga ttctggcaac ccatctttct gataatgctg
    16261 tcgatttccg cgaaattgat tacactcgcc cgacctgcat tttgatggga caggagaaaa
    16321 cgggcatcac gcaggaagca ttggccctgg cggatcagga catcatcatt ccgatgatcg
    16381 gcatggtgca gtcgctgaat gtttccgttg cctcagccct cattctttac gaagcccagc
    16441 gtcagcggca aaatgcaggc atgtacctgc gtgaaaacag catgttgccg gaagcagagc
    16501 aacaacgcct gttgtttgaa ggcggctatc cggtgctggc gaaagtcgca aaacgcaaag
    16561 gcctgcctta tccccacgtc aatcagcaag gcgagatcga agctgatgcc gactggtggg
    16621 ctactatgca ggctgcaggg taagtgccat gaaaggtcgc ctgttagatg ctgtcccact
    16681 cagttcccta acgggcgttg gcgcagcact tagtaacaaa ctggcgaaaa tcaacctgca
    16741 taccgtgcag gatctactct tacaccttcc cctgcgctac gaagatcgca cccatctcta
    16801 ccccatcgga gaactactgc cgggcgttta tgccacggtg gaaggcgaag tgctgaactg
    16861 caatatctcc ttcggcggtc ggcggatgat gacctgccag atcagcgacg gttccggcat
    16921 cctcaccatg cgctttttca atttcagcgc ggcaatgaaa aatagcctgg cgacgggccg
    16981 ccgtgtactg gcttatggcg aagcaaagcg cggtaaatat ggtgcggaga tgatccaccc
    17041 ggaataccgc gtgcagggcg atctcagcac gccagaatta caggaaacgc tcacgccggt
    17101 ttatccaaca acggaaggcg taaagcaggc cacgctgcgt aaattaaccg accaggcgct
    17161 ggatctgctc gacacctgcg ccattgaaga actcctgccg ccggaactgt cacaaggaat
    17221 gatgacgcta ccggaagcgt tgcgcacttt gcaccgcccg ccaccgacgc tacagcttag
    17281 cgatctggaa accgggcagc atccggcgca acgtcgtctg attctggaag aactgctggc
    17341 gcacaacctc agcatgttag ccttacgtgc cggagcacag cgttttcatg cccagccgct
    17401 gagcgccaat gacacgctga aaaataaact cctcgccgcc ttaccgttca agccaacggg
    17461 cgcacaggca cgcgtagtgg cggagatcga gcatgatatg gcgctggatg tgccgatgat
    17521 gcgcctggta cagggcgatg taggttccgg taaaacgctg gtcgccgccc tcgccgcgct
    17581 gcgtgcgatt gcccacggca aacaggtggc actgatggca ccaaccgaat tactcgccga
    17641 gcagcacgcc aataacttcc gcaactggtt tgcaccgctc ggtatcgaag tgggctggct
    17701 cgccggtaag cagaaaggca aagcgcgact gtctcagcag gaagccatcg ccagcggtca
    17761 ggtgcagatg attgtcggta cacacgccat cttccaggaa caggtgcagt ttaacggcct
    17821 ggcgctggtg attatcgacg aacagcatcg ttttggcgtg catcagcgtc tggcattgtg
    17881 ggagaaaggc cagcagcagg gcttccatcc gcatcagttg atcatgaccg ccacgccgat
    17941 cccccgcacg ctggcaatga cggcgtatgc cgatctcgat acctcggtga tagatgagct
    18001 gccgccaggc cgcacgccag tgactacggt cgctattcct gatacccgcc gtaccgacat
    18061 cattgaccgc gtgcgccacg cctgcataac tgaaggtcgt caggcgtact gggtttgtac
    18121 gttgattgaa gagtcagagt tactggaagc gcaggcggcg gaagctacct gggaagagtt
    18181 gaaactggcg ctaccagaat tgaatgttgg cctggtacac gggcggatga aaccagccga
    18241 gaaacaggcg gtgatggcgt cgtttaaaca aggtgagcta catctgctgg ttgccactac
    18301 cgttattgaa gtcggtgttg atgtgcctaa cgccagcctg atgattatcg aaaaccctga
    18361 gcgtctgggt ctggcgcagt tacaccagct gcgcgggcgc gtaggtcgtg gcgcggtggc
    18421 ttctcactgc gtgctgctct acaaaacgcc gctttctaaa acggcgcaaa ttcgcctgca
    18481 agtgctgcgc gacagtaacg acggttttgt gattgcgcaa aaagatctgg agattcgcgg
    18541 ccctggcgaa ttgttaggca cgcgtcagac gggtaatgct gaatttaaag tggcggattt
    18601 actgcgcgat caggcgatga tcccggaagt tcagcgcctg gcgcgacata ttcacgaacg
    18661 ttacccacaa caggcaaaag ccttgataga acgctggatg ccggagacag aacgttactc
    18721 gaatgcgtaa aaggcggcgg tgagaagacc gccgcttcat cggttaaccg gcaaaaatcg
    18781 gcaacattaa atacagctta atcaccagcg cattgacgat atcgataaag aacgcaccga
    18841 ccatcggcac caccaaaaac gccatgtgcg acgggccaaa gcgttcagtg atcgcctgca
    18901 tgttggcgat tgccgttggc gttgcaccga ggccaaaacc acagtgaccc gcagccagca
    18961 ctgccgcatc gtagtttttg cccatcatgc gccaggtaac gaagatggca tacaacgcca
    19021 tgaagatggt ctgtaccacc agaatcgcca gcatcggcag cgccagcgaa gccagctccc
    19081 acagtttcag ccccatcaac gccatcgcca ggaacaacga caagcttacg ttacccagca
    19141 cggataccgc acgctcaaag acgcggtaaa agcccattat tgacagaccg ttgctcagaa
    19201 tcacgccaac aaacagtaca cagacgaagg tcggcagttc aaaagcagtg ccagccaaaa
    19261 gttgcgcaac aattttcccc accgtcaggc agatagcaat cagcgcgata gtttcaatca
    19321 gcaccaacga ggtgatcatg cgtcccacat ccggcttttc aaacgccgtc gggacttcct
    19381 ggtcatccgg aataccgttc ggcgtggtgg agtgtttcac cagatagcgc gccaccggac
    19441 cgccaatcaa gccgcccagc accagaccga acgttgcaca ggccatcgcc acttccgtcg
    19501 cattggtgaa gccataacgt tcaatgaaca atttactcca cgcagcgccc gtaccgtgac
    19561 cgccggaaag cgtaatagaa ccggccaaca gccccatcag cggatcaagc cctaacaggc
    19621 tagccatacc aatgccaatg gcattttgca tcaccaacag accaacaacc acaatcaaga
    19681 agatgccaac cacacgccca ccggcacgca aactggcaat gttggcgttc aggccaatgg
    19741 tggcgaagaa agccagcatt aacggatcgc gcagggacat atcaaagttg acttcccagc
    19801 ccatgctttt tttcagtact agtagcgcca gcgccaccaa caaaccaccc gcaacaggtt
    19861 ccggtatggt gtatttcttc aaaaaggaga cggaatggac caacttacgc ccgagcagca
    19921 acgtcagcgt tgcggcaaca agcgttgcta aagtatcgag atgaaacata gttactcctt
    19981 tgtatccgca tcactctttc atactcgtca tacttcatcg cgcagtggca acttcatgaa
    20041 ctatgacggg tatcgagtta tgctttcctg ggtcaaattc tcagctgtta atgaacaaat
    20101 ggcatggatt ttaagcagaa aaataccaaa agttatataa aaaagtgcat ttatctcatt
    20161 taaatattta gatgaggatg tggcaaacgt ttgcttttct ctttcggtca gctaaaatgc
    20221 ccgctttgct accacgggat tgttttcgat gtctgtttcc accctcgagt cagaaaatgc
    20281 gcaaccggtt gctcagactc aaaacagcga actgatttac cgtcttgaag atcgtccgcc
    20341 gcttcctcaa accctgtttg ccgcctgtca gcatctgctg gcgatgttcg ttgcggtgat
    20401 cacgccagcg ctattaatct gccaggcgct gggtttaccg gcacaagaca cgcaacacat
    20461 tattagtatg tcgctgtttg cctccggtgt ggcatcgatt attcaaatta aggcctgggg
    20521 tccggttggc tccgggctgt tgtctattca gggcaccagc ttcaactttg ttgccccgct
    20581 gattatgggc ggtaccgcgc tgaaaaccgg tggtgctgat gttcctacca tgatggcggc
    20641 tttgttcggc acgttgatgc tggcaagttg caccgagatg gtgatctccc gcgttctgca
    20701 tctggcgcgc cgcattatta cgccgctggt ttctggcgtt gtggtgatga ttatcggcct
    20761 gtcgctaatt caggtggggc tgacctccat tggcggcggt tacgcagcca tgagcgataa
    20821 caccttcggc gcaccgaaaa atctgctgct ggcaggcgtg gttttagcct taattatcct
    20881 gcttaaccgt caacgtaacc cttacttacg cgtggcctca ctggtaattg cgatggcggc
    20941 cggatatgcg ctggcgtggt ttatgggcat gttgccagaa agcaacgaac cgatgacgca
    21001 agaactgatt atggtgccaa cgccgctcta ttacggtctt ggcattgaat ggagtctgct
    21061 gctgccgctg atgctggtct ttatgatcac ttcgctggaa accattggcg atatcacggc
    21121 gacctctgac gtttccgaac agccagtgtc cggtccgctg tacatgaaac gcctgaaagg
    21181 cggcgtgctg gcaaacggcc tgaactcgtt tgtttcggcg gtgtttaaca ccttcccgaa
    21241 ctcctgcttc gggcaaaaca acggagtgat ccagttgact ggtgttgcca gccgctatgt
    21301 cggttttgtc gtcgcgctga tgttgatcgt gctgggtctg ttcccggcag tgagcggttt
    21361 tgtacaacac attccagaac cggttctggg cggcgcaacg cttgtaatgt ttggcaccat
    21421 cgccgcctcc ggtgtgcgta tcgtttctcg tgagccgctg aaccgtcggg cgattctgat
    21481 tatcgcgctg tcgctggcgg ttggtctggg cgtgtctcag cagccgctga ttttgcagtt
    21541 tgcccctgaa tggctgaaaa acctgctctc ctccgggatc gccgcgggcg gtattactgc
    21601 catcgtgctg aatctgattt tcccaccaga aaaacagtaa ttcactggcg gcggtaagat
    21661 aattaccgcc gcagtctcac ctctttcacc attcccgcct tgaggattgc gcgtaaatcg
    21721 tgcataactc ccttatgtgc atttcacggg atggaagacc atgaaattta ttgggaagct
    21781 gcttctctac attctcatcg ctctgttagt ggtgatcgct ggcctctatt ttcttctgca
    21841 aacccgctgg ggagcagaac atatcagcgc atgggtttcc gagaatagcg actatcatct
    21901 ggccttcggg gcgatggatc accgtttttc cgcgccatct catatcgtgc tggagaatgt
    21961 cacgtttggt cgtgatggtc agcccgcgcc cctggtggca aaaagtgtcg acattgcgct
    22021 aagcagtcgc caactgaccg aaccacgcca tgtcgatacc atcctgctgg aaaacgggac
    22081 gctgaatctc accgaccaga ccgcgccgct accgttcaaa gccgatcgtc tgcaactgcg
    22141 tgatatggcg tttaatagcc cgaatagcga atggaaactg agcgcgcagc gggtaaatgg
    22201 cggcgtggtt ccgtggtcac cagaagccgg taaagtgctg ggtacgaagg cgcagattca
    22261 gtttagtgcc ggatcgcttt cgctcaatga tgttcctgcc accaatgtac tgattgaagg
    22321 cagtattgat aacgatcgcg ttacgctgac taacctgggt gccgacatcg cccgcgggac
    22381 attaaccgga aacgcgcagc gtaacgccga cggcagctgg caagtggaaa atctgcgcat
    22441 ggcggatatc cgtctacaaa gcgaaaaatc gctaaccgac ttctttgcgc cattacgctc
    22501 tgtcccgtcg ttgcagattg gtcgcttgga agtcatcgat gctcgtctgc aaggtccgga
    22561 ctgggcggtg accgacctcg atctcagctt gcgcaacatg accttcagta aagatgactg
    22621 gcagacacag gaaggcaaac tgtcgatgaa cgccagcgag ttcatttatg gttcgctgca
    22681 tttatttgac ccgattataa acgcggaatt ttccccgcag ggcgtagcgc tgcgccagtt
    22741 caccagccgc tgggaagggg gtatggtcag aacgtcaggg aactggctgc gtgacggtaa
    22801 aacgttgatc cttgatgatg cggcaattgc cgggctggaa tataccttgc cgaaaaactg
    22861 gcaacagttg tggatggaaa cgacacccgg ttggttaaac agcctgcaac tgaagagatt
    22921 tagcgccagc cgcaatctga tcattgatat cgaccctgac ttcccgtggc agctcaccgc
    22981 gctcgatggt tacggtgcca acctgacgct ggttaccgat cataaatggg gcgtctggag
    23041 tggctcggcg aatctgaatg ccgccgccgc gacattcaat cgtgttgatg ttcgtcgccc
    23101 gtcgctggcg ctgaccgcca acagcagcac ggtgaatatc agcgaactga gtgcatttac
    23161 tgaaaaaggc attctggaag ccaccgcaag tgtttcacaa acgccacaac gtcagaccca
    23221 tatcagcctg aatggacgcg gtgtgccggt gaatattttg caacaatggg gatggcctga
    23281 attaccgttg actggcgacg gcaatattca gcttaccgcc agtggagata ttcaggccaa
    23341 tgccccgctg aaacctacgg ttagcgggca attgcatgcc gtgaacgccg caaagcagca
    23401 agtgactcaa accatgaatg cgggcgtcgt ttccagcggt gaagttacat cgacggagcc
    23461 ggtgcggtaa acatcaatct cgccgggtac gactcagacg tacccggcat tccgtcaata
    23521 gattacaacg taattgtcag cgcattccct tgaggcgtca ccaccagccc ctgctcactt
    23581 tcagcctgcg aaccgccttg cagaccattt actttcacaa cattgcgcaa gcacagcgtc
    23641 cagttcttcg cctcgcccgc accagtcaca gtaatcgtgt tgccagtacg tgctgctttt
    23701 aaagtaaaga tcaccgatcc gtcagcagca ggcacttcac agacggcttc atgcccgtct
    23761 tgcagattaa agaggtggaa tgccgtgcct tcgtgccaca cgtaatcggg acgttgatcg
    23821 ttgttgccca gcgccagtag agtgttatca cgcacataaa cgggcagact caggaagccg
    23881 tgctgctgtt tatgccagcg actaccgtcg agttcatcgt tgtgccacag gtgtgtccag
    23941 cgaccttccg gcaggtagaa ctgcacatcg cccgcttcag tgaacaccgg cgcaaccatc
    24001 acgttgtcgc ctaacatgta ttgacggtca aggtaatcac aagccggatc gtccgggaac
    24061 tccatcatca tggcccgcat catcggcgta ccccgcgcgt tcgcacgcgc agcttcacga
    24121 tacagatacg gcatcatgcg gcatttcagt tgcgtgaaga agcgcaccac atcacaggac
    24181 tcatcatcgt aggcccacgg cacacgataa gatttgctac cgtgtaaacg gctatggctg
    24241 gagagcaaac caaacgcgca ccagcgtttg taaacgtgcg ccggagcggt attttcaaag
    24301 ccgccgatat cgtggctcca gaagccaaaa cctgaaaggc caatagacaa accaccgcgc
    24361 aggctttccg ccattgattc gtagttagcg taacaatcgc caccccagtg taccgggaat
    24421 ttctgcgcac cgacggaggc cgagcgggca aacaagacag cttcttcctc accaacggtg
    24481 tccttgagca cgttccacac cagttcgttg tagatgtacg cataatggtt atgcattttc
    24541 tgcgaatcgg aaccgtcaaa ccactgaaca tcaattggga tacgttcgcc aaagtcggtc
    24601 ttaaagcaat caacgcccat cgcgaccaga cctttcagtt tgtcggcgta ccatttgcag
    24661 gcatccggat tggtaaagtc ataaatcgcc agacctggct gccatttatc ccactgccat
    24721 agcgaaccgt ccgggcgttt gagtaaatag cctttctctt gtaactcttt aaagacgggg
    24781 gatttttgac cgatataggg gttaatccag acgcagattt tcagcccttt cgctttcagg
    24841 cggcggatca tcccttccgg gtcagggaaa gtcagcgggt cccactcaaa atcgcaccac
    24901 tggaaggctt tcatccagaa acagtcaaag tggaaaacat gcagcggcag attgcgttcc
    24961 gccataccat cgataaagct gtttaccgtc gcttcgtcgt agttggtggt aaatgaagtg
    25021 gttagccaca ggccgaagga ccacgggggc ggcagcgccg gacgaccagt aaagcgggta
    25081 taacgatcaa gtaccgcttt cggcgtcggg ccgtcgataa caaagtattc gagatattca
    25141 ctctcaacgc tgaactgcac tttggagact ttctccgatc ccacttcaaa agagacacac
    25201 tggggatgat tgaccagtac cccataacca cggttagtca tgtagaacgg gatattttta
    25261 tacgcctgtt cagtacttgt gccgccgtcc cggttccagg tctctaccgt ctggccattg
    25321 cgcaccaggg cagtaaagcg ctctcccaga ccgtaaactg tttcgccaac gccaagatca
    25381 agccgctcaa acatataatt gcgttgatta ttcgtgtcct gcacgtagcc attatttttc
    25441 acctgactac cggtaatacg ttcgccgttg cgcagaaaat ccagtgacca gaactcacct
    25501 ttgctgacac gcgcgcttaa gttgccactt ttaaactcag cataacgttc tgtgttttcg
    25561 attgtgacct tcacgtcctg caaaatattg agcggataat gaggaccgtt attcagcgcc
    25621 ccctgaaaat gctcaatccg cacaccgaca ataccttcct gtggggagaa aaagcgcagc
    25681 gtaaataaag gcgtatcaag ctgccaggta cgttcacgca catcacgggg ggcagcatag
    25741 accaccattt cattatcctg ctgttcaacc tcgaatacct gaagcgggtg aatcaaattg
    25801 aggccaggtt gaatcaacca gtttccatcg ctaattttca ttgccgttcc ttagttctgc
    25861 acttcttgag aggtgaattg ttgctggcaa cggcgtttac cctgagccag ctgttccata
    25921 acggttttca gattgtgcgt cgtgagtgag tagtagcgtt tagcgataat cgcgctcagc
    25981 aaataacaga tcgccggaac aatcgtgaat agcgcaataa tgatgctaat cgtggcgctg
    26041 ttctgcgctt tttctgccgc atcatatccg ccataagcca gcatccagcc gataagagcg
    26101 ccgccgaagg ccaaccccag tttgagcaca aacagcgtgc cagcaaaact gatcccggtc
    26161 aggcgtttac cattgcacca ctcgccgtag tcgacggtat cgaacatcat tacccactgg
    26221 ataggtgtca ccagttgatg caacacacca atcacgaaga tgaagacaaa catagtgatg
    26281 ctggcctgca tgggaacaaa gaacatcgcg aggctaatca ctgccagcag ggcgttcgtc
    26341 caccagaaga tagtgacttt acatttccag tcggtcagag gttttgccag tgcggaacca
    26401 atcaggttac ccacgcaata agtggtgaga aaagcgacaa acacttccgg cgtgcccaaa
    26461 atccatgtga cgtaatacat catcgcccca ccgcgtacgc acaccgccag gatattgaaa
    26521 atggttagta aaccgacaat ccgccactgg tcgttttgcc agatatcacg taaatcttcc
    26581 cgcatagacg ttgttgtagg tggtgcttca acgcgttctt tagtggtgaa gaaacaaaat
    26641 gccagcatca tgaatgccac cacggaaagg accacgatac cgccctggaa accgagtggt
    26701 ttattatcac cgccaattaa attaaccagt ggcatcatca gaacagtaga aagcatgcct
    26761 cccgcggtcg ccagcacaaa acgccaggat tgcagcgaga tacgctgagt cgggtcattg
    26821 gtgattacac cacccaatgc gcagtaaggg atattgacga cggtataaag taaggtaagt
    26881 tagggtgtaa gtaattgctg catagatcat tttgccgttc atactgagat ctggcgtgct
    26941 ataggccagt acacagacga tcccgaatgg cagtgcgcca aacagtaccc acggacgaaa
    27001 tttaccccag cgagagcgcg ttcgatcggc caacaacccc atgcaaggat cggaaatcgc
    27061 atccagtgca cgagcgacca aaaacatggt tccgacaaat ccggcaggaa tgccaaaaat
    27121 atcggtataa aagaacatca tatataacat tacgttatcg aaaataatgt ggctggcggc
    27181 gtctcccatg ccataaccaa ttttctcttt aacggacaac acttcactcc tcatctgctt
    27241 ttccttgatg ctataggggt gctgagaccg ataacgcatt ttgtaaacca tccctgtgag
    27301 agcgaatatt ccgatatctg gttattaatt tatgattctt gttttatgtg atcgtggtag
    27361 cgttaattcc gctcatatat cattgtaaaa tatgggtttt atataaatta taatgctttc
    27421 gtgataatac gctgcgttta ttaggcggaa aaaactgatc tgggggatgt agaaactcaa
    27481 ggaagtagct ataatgcgcc ccgcctccat gtagcaatcg aggcgcggaa gatcgtcgtc
    27541 tccggtgagg cggctggact tcaaatccag ttggggccgc cagcggtccc gggcaggttc
    27601 gactcctgtg atcttccgcc agtttacctc ttctgaactc tcccaatgtc aataaatctc
    27661 ttctaatccc ttgtatatca gtatttattt gtatcctgag gtcaacctac ttcaccccgt
    27721 atcaagtttc ttttgggggt actttagggg gtgttatttc ctattgaaat acaagcaccc
    27781 ccataatagg aaaaatgtga tacataacaa cggcttttaa caatatcttt gttatacaag
    27841 gggtgtattg ggggtatcag gttagttact ggcagaaatc cccctcagga acatgaattc
    27901 atcaaaagcc ggaggatgtc atggcattaa cgatatcaaa gtgaaaaccg caaaacccaa
    27961 agacaaaccc tataagcttg ctgatggcgg cggtatgtat ctactgatta acactaatgg
    28021 ttctaaatac tggcgcatga agtaccgctt tgctggtaaa gaaaagatgc tttctattgg
    28081 cgtatacccc gatgtaacgc tagccgatgc tcgcgagaaa cgcagtgaag ccaggaaact
    28141 cctcgctgct ggaggcgatc caggggaggc aaaaaaggaa gaaaaaatcg cgcagcaaat
    28201 gagtctgaaa aatacgttcg aggctattgc tcgcgaatgg caccagttaa aagctgatcg
    28261 gtggtcatta cgctatcgtg atgaaatcat tgataccttt gaaaaagaca tttttcccta
    28321 cattggcaag cgccctattg ccgaaatcaa accaatgaag ttactggaag cactgcgtaa
    28381 aatggaaaaa cgtggagctc tggagaaaat gcgcaaggtc cgccagcgct gtggtgaggt
    28441 gttccgctat gcaattgtta ctggccgggc agactataac ccagcccccg atcttgccag
    28501 tgctttagct acaccgaaaa aagtacattt tccctttctt actgctaatg aacttcccca
    28561 cttcctcaac gatctggcgg gttataccgg aagtataatc actaagacag ctactcagat
    28621 cattatgtta accggcgtac gaacacaaga actgcgtttt gcacgctggg aagatatcga
    28681 ttttgagaca aaattgtggg agatcccggc agaagtaatg aagatgaagc gacctcatat
    28741 tgtgccgctc tctgagcagg tcattatgct atttaagcag cttgaaccaa tctcaaaaca
    28801 tcatccccta gtctttatcg gtagaaatga tccccgcaaa ccaattagta aggaaagcat
    28861 taaccaagta attgaattac ttggatataa gggaagactc acagggcacg gtttcagaca
    28921 caccatgagt acaattctgc atgaacaagg ctttaattcc gcctggattg aaatgcagtt
    28981 agctcatgtg gataaaaatt ccatcagggg tacctataat catgccctgt atctcgatgg
    29041 ccgccgtgaa atgatgcaat ggtacgctga ttacattgat tcactctcca gccgggaaag
    29101 ttaagtcaga aatacttttc cctgatgttt tcctcaatta agtattttat cctctggcct
    29161 acaaggctgg aggatttttg ttattatatt atttttttgc gtcttcaaac ttattacaaa
    29221 aacataatta aaaacaaatt gacattttta aattggcaat tacaaaatac agccttgcag
    29281 ttatttcgat acctttagca aaggaatagt tgattcagat ataatttagc atttcaatca
    29341 tctgacatca ttataaaaaa tgagattcac ataaagataa aattattgtt ctcaaggaaa
    29401 gcatgaaggg gcttcgtaag caatattcat tcggtgccaa gacaggactt gatacacaga
    29461 atctcaactt ttatgctctc agactatatc accacatgtt ttacaggctc agaaaatgaa
    29521 cgatagatta tgctttgaag tgcacgataa caagggttat ttcgtatttc cggatacctg
    29581 gtttggccca ttgctggggg agtttgaaga agtgcttgat gcatacgacg ctgatgaaat
    29641 cagcgagacg agttacatca acaaactccg acgccttgct caacgggagc cggactttat
    29701 tgatatacac gctcatctgg catatgcatt tctggaacag aatgccccgc gtaaggcact
    29761 gaacgccgct cttaaaggac tggcggcagg taatcgtatc atcccggaaa gtttctgtgg
    29821 tgaaataatc tggatgcatc ccgaaaaccg gccttatctg cgcgcgcttt atgcagcaat
    29881 tctcgcaaat gtgcacttac agcgccatca ggatgcggtt atgctcactg ataaaatact
    29941 ggcatacaat cctgaagata atcagggggc tcgctggttg ttgggttcag agctgttacg
    30001 caccggcgat catgagcgag ccttcagtgt actgaaagag catgctgatg aattttcacc
    30061 ttactggtat gaactcggtc tcctgcattt tctgaacggg gaacatgtta aggccgccac
    30121 agcttttcgc catggctttg ccacaaacac ttacatcgca gaaatgctgt gcggaaatct
    30181 gcatccattt ccacttgctg tgtggcatga cttctccggc tctctggata cggcagaaga
    30241 ttactatgca acatactcac cattatgggg acaatatccg gaagcattgc tgtttgtaaa
    30301 ctggctctat aaccattcct ccgtgctgca tgaacgatca gaaatcatta aatgcgccga
    30361 aatgctgatt caggaagatg atttcgaaat atgcgaaagt attttaagac agcaggagca
    30421 cctgtggaaa agaattgaca agacactttc tgaggaaatt gtacagaaat gcagaaatat
    30481 gaacggtgaa tatatctggc cctggatatt gcccttttcc gcagcaggga tgaaacattc
    30541 aagcatccag tatcagtaga tattgcatta gtgtaacttt aaaacaagta ataatttatt
    30601 atccaataat aacactgtgt tccttcccca ggtgattacc ctggggttat tttctgaaag
    30661 tttcctttgc aaaaggatag cgttgtaaat tactttaacg aattactcca tgaactcatt
    30721 aaagttcttt cgtaatactt tatcgtcaaa cacgttatag ctggcatcaa aaaccacaga
    30781 tttaccctgc cagcgggtaa tcccctcctc tggcgttttc ggttgaactg gtatccccgt
    30841 ccaacacagg cagccattat aaacagtaaa aaattgtatt cgctgttatg aaatcaatag
    30901 tcctgacgta ctgttgaatg tgcttgtgtt ctgaacctcg tacaaccagt acatttgata
    30961 tcgaattata aacaaaacat tgcatccaac ttttttaata tggacaatag ttgtccatac
    31021 ggtggtttta tattcgataa atcaatggat ttcattgagt cacaggactg gtaatggatg
    31081 aaaatgcttt aaggtttgcc tcatactggc gcaactcgct tgcagatgct gagtcaggaa
    31141 agggctgttt tgaacggaaa gacgcccaaa atttcactca ctggcatggg atagcggcgg
    31201 gacgtcttga cgaagcgatt gtcaataaat tttttaaggg agaaaaagac gatgtcgaaa
    31261 ctgtcaatgt catcttacgc ccaaaagttt atttccggtt actgcagcat ggtaaggacc
    31321 gttccgcagg cacgcctgat attgttacac cgatagtgac gccagccttg ctaagccgtg
    31381 agggtttttt atatccgacg ccagcgacct ccattcccag agacctgctt gaacctttgc
    31441 caaaaggagc attttcgatt ggtgagattg ggcagtatga caaatacaag acgacccata
    31501 ctgatcctac ccacgtaatg tggacacggc cctaagcgag attatggttt tcaaattgtt
    31561 ccgggctgag accgccacag gcactgtgac gacgccagcg attgtaatca cactcgatat
    31621 aattaaacac cgttgcccgc attatttccc ggctgctaaa gcgttccccg tggatacatt
    31681 ccaccttcag cgaatgaaag aagctttcca cacaggcatt gtcataacag tagcctttcg
    31741 cactcatact gccacgcagg ttgtgtcgct tcagcattgc ctgataatcc cctgaacagt
    31801 attgaccacc acggtccgta tgaacaatga cgctttccgg gcgtcttctc cgccacaacg
    31861 ccatttgcag tgcatcacag gccagttgtg ctgtcattcg cggtgacatc gaccagccaa
    31921 tgacggcgcg tgaccacagg tcgatgacta ctgcgagata caaccagccc tcatcggtac
    31981 gcaagtacgt gatgtcaccc gcccacttct ggtttcgggc cactggcgct gaagtcctgc
    32041 tccagcagat tctccaatac gggcaggcca tgtgcacggt agctgaccgg gctgaacttc
    32101 cggctggctt tcgcccgcag cccctgacga cgcaggctgg cggcaatggt tttaatattg
    32161 aactccggca gttcgtcagc aaggcgggga gcaccgtatc gctgctttgc ctcaaagaat
    32221 gccttatgga cagcggcatc gcaggtgagc cgaaactgtt ggcgcaggct catctggtga
    32281 cgacgcctga gccagacata ccagccgctg cgggcaaccc gaagtacacg acacatcgct
    32341 ttgatgctga actctgcccg atgattttcg ataaagacat acttcatttc aggcgcttcg
    32401 cgaagtatgt cgcggccttt tggaggatgg acagttcctc agcctgctcc gccagttgtc
    32461 gtttaaggcg gacattttca gcggccagtt cgctttcgcg ctctgacgaa ctcatttgtt
    32521 gctgctgttt actgcgccag gcataaagct gagattcata caggctgagt tcacgggctg
    32581 cggcggccac accgatgcgt tcagcgagtt tcagggcttc gttacgaaat tcaggcgtat
    32641 gttgtttacg gggcttcttg ctgattgata ctggttttgt catgagtcac ctctggttga
    32701 gagtttactc acttagtcct gtgtccacta ttggtgggta agatcaccct ccattaaaac
    32761 taaaacgggc gctttctgat atatcatctg taagcgcagt aataccatta gtagcacctc
    32821 gcgcattgat tattgaatat tataactgga atgttatatt tcttgttatg atgctgttca
    32881 gtattataat aatgttatta tcaccaaaga taactcatgc aaatgagaaa gtaatatgtc
    32941 aaaacagtgc atcctttgtg accaaagagt ttatctgcgg cactatactt gtctcattaa
    33001 tactcagttc tctattttgc tacatatcaa tatcaccatc aattttcatg catacttatg
    33061 gcctgagtac ttttagttac tcaattattt tctccttctc agttttattt ttcataatcg
    33121 gaaatcaatt atcaaagatt gaaaaattat ccaacctatg gtttttatta cccaatagcc
    33181 aattttatcg cattacaaat aaatggttca aaaacaggat ttgccgcatc tattacaggt
    33241 tttactcaaa caataagcgc tggtacaatc agtttcctgt cagtaaccgt aagcgacaac
    33301 ggtgttggtc ccggaactac tttaggtctt g
//
LOCUS       DAJTUP010000036        31780 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTUP010000036 DAJTUP010000000
VERSION     DAJTUP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.036"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..697
                     /gene="menF"
                     /locus_tag="OO468_001763"
     CDS             <1..697
                     /gene="menF"
                     /locus_tag="OO468_001763"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6258905.1"
                     /translation="FASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPERLWR
                     RRDKALRTEALAGTVANHPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQT
                     LDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLLPTAAVAGLPRDLARQFIARH
                     EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEID
                     NKAAGLRTLLQME"
     gene            786..2456
                     /gene="menD"
                     /locus_tag="OO468_001764"
     CDS             786..2456
                     /gene="menD"
                     /locus_tag="OO468_001764"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR6258906.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            2453..3211
                     /gene="menH"
                     /locus_tag="OO468_001765"
     CDS             2453..3211
                     /gene="menH"
                     /locus_tag="OO468_001765"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR6258907.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            3226..4083
                     /gene="menB"
                     /locus_tag="OO468_001766"
     CDS             3226..4083
                     /gene="menB"
                     /locus_tag="OO468_001766"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR6258908.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            4083..5045
                     /gene="menC"
                     /locus_tag="OO468_001767"
     CDS             4083..5045
                     /gene="menC"
                     /locus_tag="OO468_001767"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR6258909.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            5042..6397
                     /gene="menE"
                     /locus_tag="OO468_001768"
     CDS             5042..6397
                     /gene="menE"
                     /locus_tag="OO468_001768"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR6258910.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            6502..6774
                     /gene="pmrD"
                     /locus_tag="OO468_001769"
     CDS             6502..6774
                     /gene="pmrD"
                     /locus_tag="OO468_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR6258911.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(6768..7154)
                     /gene="arnF"
                     /locus_tag="OO468_001770"
     CDS             complement(6768..7154)
                     /gene="arnF"
                     /locus_tag="OO468_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR6258912.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7154..7489)
                     /gene="arnE"
                     /locus_tag="OO468_001771"
     CDS             complement(7154..7489)
                     /gene="arnE"
                     /locus_tag="OO468_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR6258913.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(7486..9138)
                     /gene="arnT"
                     /locus_tag="OO468_001772"
     CDS             complement(7486..9138)
                     /gene="arnT"
                     /locus_tag="OO468_001772"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR6258914.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            complement(9138..10028)
                     /locus_tag="OO468_001773"
     CDS             complement(9138..10028)
                     /locus_tag="OO468_001773"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR6258915.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(10025..12006)
                     /gene="arnA"
                     /locus_tag="OO468_001774"
                     /pseudo
     CDS             complement(10025..12006)
                     /gene="arnA"
                     /locus_tag="OO468_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            complement(12006..12974)
                     /gene="arnC"
                     /locus_tag="OO468_001775"
     CDS             complement(12006..12974)
                     /gene="arnC"
                     /locus_tag="OO468_001775"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR6258916.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(12978..14117)
                     /gene="arnB"
                     /locus_tag="OO468_001776"
     CDS             complement(12978..14117)
                     /gene="arnB"
                     /locus_tag="OO468_001776"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR6258917.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            14425..15027
                     /locus_tag="OO468_001777"
     CDS             14425..15027
                     /locus_tag="OO468_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR6258918.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(15066..15491)
                     /gene="nudI"
                     /locus_tag="OO468_001778"
     CDS             complement(15066..15491)
                     /gene="nudI"
                     /locus_tag="OO468_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR6258919.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            15770..16312
                     /locus_tag="OO468_001779"
     CDS             15770..16312
                     /locus_tag="OO468_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6258920.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16399..17601
                     /locus_tag="OO468_001780"
     CDS             16399..17601
                     /locus_tag="OO468_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR6258921.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            17656..18126
                     /locus_tag="OO468_001781"
     CDS             17656..18126
                     /locus_tag="OO468_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR6258922.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(18167..19117)
                     /locus_tag="OO468_001782"
     CDS             complement(18167..19117)
                     /locus_tag="OO468_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR6258923.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(19310..20500)
                     /gene="glpC"
                     /locus_tag="OO468_001783"
     CDS             complement(19310..20500)
                     /gene="glpC"
                     /locus_tag="OO468_001783"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR6258924.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(20497..21756)
                     /gene="glpB"
                     /locus_tag="OO468_001784"
     CDS             complement(20497..21756)
                     /gene="glpB"
                     /locus_tag="OO468_001784"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR6258925.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(21746..23374)
                     /gene="glpA"
                     /locus_tag="OO468_001785"
     CDS             complement(21746..23374)
                     /gene="glpA"
                     /locus_tag="OO468_001785"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR6258926.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            23647..25005
                     /gene="glpT"
                     /locus_tag="OO468_001786"
     CDS             23647..25005
                     /gene="glpT"
                     /locus_tag="OO468_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR6258927.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            25010..26086
                     /gene="glpQ"
                     /locus_tag="OO468_001787"
     CDS             25010..26086
                     /gene="glpQ"
                     /locus_tag="OO468_001787"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR6258928.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(26128..26370)
                     /locus_tag="OO468_001788"
                     /pseudo
     CDS             complement(26128..26370)
                     /locus_tag="OO468_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            26549..27199
                     /gene="inaA"
                     /locus_tag="OO468_001789"
     CDS             26549..27199
                     /gene="inaA"
                     /locus_tag="OO468_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR6258929.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(27253..27507)
                     /gene="yfaE"
                     /locus_tag="OO468_001790"
     CDS             complement(27253..27507)
                     /gene="yfaE"
                     /locus_tag="OO468_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR6258930.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(27507..28637)
                     /gene="nrdB"
                     /locus_tag="OO468_001791"
     CDS             complement(27507..28637)
                     /gene="nrdB"
                     /locus_tag="OO468_001791"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR6258931.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(28871..31156)
                     /gene="nrdA"
                     /locus_tag="OO468_001792"
     CDS             complement(28871..31156)
                     /gene="nrdA"
                     /locus_tag="OO468_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR6258932.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         7568 a         7932 c         8557 g         7723 t
ORIGIN      
        1 tttcgcaagt ccggtcaacg cggcggcgat gatggctgcc agtcgtcgac tgaatctgaa
       61 ttgctaccat ttttacatgg cctttgatgg cgaaaatgct tttcttggct cttcaccgga
      121 acggttatgg cggcggcgtg acaaagcgct gcgtactgaa gcgctggcgg gaacagtagc
      181 aaatcatcct gatgataagc aggcgcagca gttaggagag tggctgatgg cggatgataa
      241 aaaccagcgc gagaacatgc tggtggtgga agatatctgc caacgattac aggccgatac
      301 ccagacgctg gatgttttac cgccgcaggt actgcgtctg cgtaaagtgc agcatcttcg
      361 ccgctgtatc tggacttcac tcaacaaagc ggatgatgtg atctgtttac atcagttgct
      421 gccaacggca gcagttgctg gcttaccgcg cgatctggcg cgacagttta tcgcccgtca
      481 cgaaccgttc acccgagaat ggtacgccgg ttctgcgggc tatctctcat tacaacaaag
      541 cgaattctgc gtttccctgc gctcagcaaa aattagcggc aatgtcgtgc gattatatgc
      601 tggcgcgggc attgtccgtg gttccgaccc cgagcaagag tggcaggaaa tcgacaacaa
      661 agcggcaggg ctgcgtactt tattacaaat ggaatagtaa tgagtcgcat cattaccgat
      721 tcatatcaat aatctatttt tgtagctcct tatacttagc cgcaatattg ataccggaca
      781 aactcatgtc agtaagcgca tttaaccgac gctgggcggc ggtcattctg gaagcattaa
      841 cgcgtcacgg cgtcagacac atctgtatcg ccccaggctc gcgttctaca ccgttaacgt
      901 tagcggcggc ggagaattcc gcattcattc accacaccca tttcgatgag cgtgggttgg
      961 ggcatctggc gctggggctg gcgaaagtca gcaagcagcc ggtggcggtg attgtgacct
     1021 ccggcacggc ggtggcaaat ctctatccgg cactgattga agccgggtta accggagaaa
     1081 aactgattct cttaaccgcc gatcgcccgc cggagctaat tgactgcggc gcgaatcagg
     1141 caattcgcca gccgggaatg ttcgcctctc accccacgca cagtatttca ttgccgcgcc
     1201 cgacccagga tatccccgca cgttggctgg tttctaccat cgaccacgct ctcggtacgc
     1261 ttcatgcggg gggagtccat atcaactgcc cgtttgctga accgctgtat ggcgaaatgg
     1321 acgataccgg gcttagctgg caacagcgtc tgggtgactg gtggcaggac gacaaaccgt
     1381 ggctgcgtga agcgcctcgt ctggaaagtg aaaaacagcg cgactggttc ttctggcgac
     1441 aaaagcgcgg cgtggtggtt gccgggcgca tgagtgcgga agagggcaaa aaagttgccc
     1501 tgtgggcgca aactcttggc tggccgctga ttggcgatgt gctgtcacaa accgggcagc
     1561 cgctgccgtg tgccgatctt tggttaggca atgccaaagc gaccagcgag ctgcagcagg
     1621 cgcaaattgt ggtgcaactg ggaagtagcc tgacggggaa acggctcctg caatggcagg
     1681 caagctgtga accagaagag tactggattg ttgatgacat tgaagggcga cttgatccgg
     1741 cacaccatcg cggacgtcgc ttaattgcca atattgccga ctggctggag ctgcatccgg
     1801 cagaaaaacg ccagccctgg tgcgttgaaa tcccgcgcct ggcggaacag gcaatgcagg
     1861 cggttattgc ccgccgtgat gcgtttggcg aagcgcaact ggcgcatcgc atcagcgact
     1921 atctgcctga acaggggcaa ttgtttgttg gtaacagcct ggtggtacgt ctgattgatg
     1981 cgctttcgca acttccggca ggttacccgg tgtacagcaa ccgaggggcc agcggtatcg
     2041 acggactgct ttcgaccgcc gcaggcgttc agcgggcaag cggcaaaccg acgctggcga
     2101 ttgtgggcga tctctccgcg ctttacgatc tcaacgcgct ggcgttattg cgtcaggttt
     2161 ctgcgccgct ggtattaatt gtggtgaaca acaacggcgg gcaaattttc tcgctgttgc
     2221 caacgccgca aagcgagcgt gagcgtttct atctgatgcc gcaaaacgtc cattttgagc
     2281 acgccgccgc gatgttcgag ctgaaatatc atcatccgca aaactggcag gaacttgaaa
     2341 cggcatttgc cgacgcctgg cgcacgccaa ccaccacggt gattgaaatg gtggttaacg
     2401 acaccgatgg tgcgcaaacg ctccagcaac ttctggcgca ggtaagccat ttatgatcct
     2461 gcacgcgcag gcaaaacacg gaaaactagg tttaccctgg ctggtgtttt tgcacggttt
     2521 ttccggcgat tgccacgaat ggcaagaagt gggcgaggcg tttgccgact actcacggtt
     2581 gtatgttgat ctcctaggtc atggtggttc ggcggcgatt agcgtcgatg gatttgatga
     2641 tgtcaccgac ttactgcgta aaaccttggt tagttacaac atccttgact tctggctggt
     2701 ggggtactcg cttggcggac gggtggcgat gatggcggct tgccaggggc tggcggggct
     2761 ttgtggggtt attgtcgaag gcgggcatcc ggggctgcaa aatgctgaac aacgtgcgga
     2821 acgtcagcgt tccgatcgcc aatgggcgca gcgttttcgc acagaaccgt taacggcggt
     2881 atttgccgac tggtatcaac agcctgtttt tgcctcgctc aatgacgatc aacgccggga
     2941 gctggtggcg ctgcgcagca acaataatgg cgcaaccctt gccgccatgc tggaggcgac
     3001 ttctctcgcc gtccagcctg atttacgtgc taaccttagc gcccgcacat ttgcgtttta
     3061 ttatttatgt ggtgaacgtg acagcaaatt ccgcgccctg gcggcggaac tggctgccga
     3121 ctgccatgtc attcctcgcg ccggacataa cgcgcatcgg gaaaatcccg ctggcgtaat
     3181 cgcaagtctg gcgcagatct tgcgtttctg actaaaggac acaatatgat ttatcctgat
     3241 gaagcaatgc cttacgcacc ggttgaatgg cacgactgct ccgaaggttt cgaggacatt
     3301 cgttatgaaa aatccaccga cggtatcgca aaaatcacca ttaatcgtcc gcaggtgcgc
     3361 aatgccttcc gtcctctgac ggtaaaagag atgatccagg cactggcaga tgcgcgttat
     3421 gacgacaata tcggcgtgat cattctgact ggcgcaggcg ataaagcgtt ctgctccggt
     3481 ggtgaccaga aagtgcgtgg tgattacggc ggctataaag atgattccgg cgtacatcac
     3541 ctgaacgtgc tggacttcca gcgtcagatc cgtacctgtc cgaaaccggt tgtcgcgatg
     3601 gtggctggct actccatcgg cggtggtcac gttctgcaca tgatgtgcga cctgactatc
     3661 gcagcagata atgccatctt cggtcagact ggcccgaaag tcggttcctt cgacggcggc
     3721 tggggcgctt cctacatggc tcgtatcgtc gggcagaaaa aagcgcgaga aatctggttc
     3781 ctgtgccgtc agtacgacgc aaaacaggcg ctggatatgg gccttgtcaa caccgtggta
     3841 ccgctggcgg atctggaaaa agaaaccgtc cgttggtgcc gcgaaatgct gcaaaacagc
     3901 ccgatggcgc tgcgctgcct gaaagctgcg ctgaacgccg actgtgacgg gcaggcgggg
     3961 ctgcaggagc tggcgggcaa cgccaccatg ctgttctaca tgacggaaga aggtcaggaa
     4021 ggtcgcaacg ctttcaacca gaaacgtcag cctgatttca gcaaatttaa acggaatccg
     4081 taatgcgtag cgcgcaggta taccgctggc agatccccat ggacgcgggg gtggttctgc
     4141 gcgacaggcg gttaaaaacc cgcgacgggc tgtacgtttg cctgcgtgag ggcgagcgcg
     4201 aagggtgggg ggagatctcc ccactgccgg gcttcagcca ggaaaactgg gaagaggcgc
     4261 aaagcgtgct gcttgcctgg gtaaataact ggctggcagg cgattgcgag ctaccgcaga
     4321 tgccttccgt ggcctttggc gtaagctgtg cattggcaga actgacagat acgttgccgc
     4381 aagcagccaa ctaccgtgcg gcaccgctgt gtaatggcga tccggacgat ctgatcctca
     4441 aacttgccga tatgccgggc gagaaagtgg cgaaggtcaa agtgggattg tacgaagcgg
     4501 tgcgcgacgg catggtggtg aatctgttgc tggaggcaat tccggatctg catttgcgtc
     4561 ttgacgcaaa tcgcgcctgg acaccgctga aaggtcagca gtttgccaaa tatgttaacc
     4621 cggattatcg ccaccgcatc gcgtttctcg aagagccgtg caaaacccgc gatgattcgc
     4681 gagcgtttgc ccgtgaaacc ggcattgcca ttgcctggga tgaaagcctg cgcgagccgg
     4741 attttgcctt tgtggctgaa gagggcgtgc gcgcggtagt tatcaaaccc acgctcacgg
     4801 gcagtctgga aaaagtacgc gagcaggtac aggcggcgca tgcgctgggg ctgacggcgg
     4861 tgatcagttc ttccattgag tcgagcttag gtctaacgca actggcgcgg attgctgcct
     4921 ggttaacgcc agacaccatt ccgggactgg acacgctgga tctgatgcag gcgcagcagg
     4981 ttcgtcgctg gccggggagc ccactgccgt tagtggacgc tgatgtacag gagcagttgt
     5041 tatgatcttc tctgactggc cgtggcgtca ctggcggcaa gtgcggggag aagccatcgc
     5101 cttacgtctt aatgacgagc aactcaactg gcgcgagctt tgtgctcgcg tcgatgagtt
     5161 agctacgggc tttgcggcgc agggggtggt tgagggcagc ggcgtgatgt tgcgggcgtg
     5221 gaatacgccg caaacgctgc tcgcctggct ggcgttactg caatgcgggg cgcgggtgtt
     5281 gcccgtgaac cctcagctgc cgcaaccgtt gcttgaagaa ttgctgccca atctgacgct
     5341 gcaatttgcg ctggtgccgg atggggaaaa cacgtttccg gcattaacgt cgctgcacat
     5401 tcagctggtt gaaggcgtac atgccgctgc gtggcagccg acgcgtctgt gctcaatgac
     5461 gttgacctcg ggttctaccg gtttgccgaa agccgctgtc catacttatc aggcccatct
     5521 tgccagtgcg caaggtgtgt tatcgctgat tccgtttggc gatcacgatg actggttgct
     5581 ctctttaccg ctgtttcacg tctccggtca gggaattatg tggcgctggt tatacgctgg
     5641 tgcgcggatg acggtgcgtg ataaacagcc attggagcaa atgctggcag gctgtactca
     5701 cgcttcactg gtgccaacac aactctggcg tttgctggtt aaccgcagtt ccgtttccct
     5761 gaaagcggtg ttacttggcg gcgcggctat cccggtcgag ttgacggaac aggcgcgcga
     5821 gcaggggatt cgttgctttt gcggctatgg tctgaccgag tttgcctcca cggtatgtgc
     5881 gaaagaagcc gacggcctgg cagacgttgg tttgccgctg ccgggtcggg aagtgaaaat
     5941 cgttaataat gaagtgtggc tgcgggctgc cagtatggca gaaggttact ggcgtaacgg
     6001 gcaactggtt tcactggtta atgacgaagg ctggtacgct acgcgcgatc gcggtgagat
     6061 gcataatggc aagctgacca ttgtcggacg tttagacaat ctgttcttca gtggcggtga
     6121 gggtattcag ccggaagaag tcgagcgtgt tattgctgca catcctgcgg ttttgcaggt
     6181 gtttatcgtt tccgttgccg acaaggagtt tggtcatcgg ccggtagcgg tggtggagta
     6241 tgaccagcaa accgttgatc ttggcgaatg ggtgaaagat aagctggctc gttttcaaca
     6301 accggtgcgc tggctgactt taccgccgga gctgaaaaac ggcggtatta aaatttcacg
     6361 tcaggcgcta aaagagtggg tgcaacgtca gcaataatta accctccgtt tcacttctca
     6421 tttctgccat tccattgccg ttgataaaga gtttatctgt taatctgtaa attgatgtga
     6481 aaactcttag caaacaggat aatgcaatgg aatggctggc acaaaaaatc gtgctgcaat
     6541 aaacaagata acagacatgt acttatgctc tgcgatgctg gcggcgcgat aaaaatgatc
     6601 gccgaagtga agagtgactt cgccgtgaaa gtgggggatt tactctcgcc tttgcagaat
     6661 gcgctttatt gtattaatcg tgaaaagctg catacggtga aagtcctctc ggcaagcagt
     6721 tatagccccg atgagtggga acggcagtgt acagcggcag ggaaaactca gtaacgttgt
     6781 tttgtcgtgg gcaggaaaat cagcatcaac ccgctcataa tacaggctac tcccagtagt
     6841 gctttcagcg aaaaagtccc ttcccagccg ggaagtacca ttgaagcaat ccataccagc
     6901 acataactca tactgagcaa ggcataggct ttgcttagcg caagtttatg tagcgtttta
     6961 taccaacaaa aaacggaaag cagataaccc agcagcccga gtaacagtat ccgggcatcc
     7021 aggccaaaag ctaacagagc cgcgataaaa tcccataagt gcgtcatcgg cggcagatga
     7081 cttgccgcaa aacccaggct taattgcgca actgaggcaa taatgacgct gaataatccc
     7141 catatcaggc acattacacc gtactcccga ggatcacaat gccaccaata atgaacgcca
     7201 ccccacacca gtgacgcggc gataccggtt cgtgccacag ttttactgca gccagcgtca
     7261 cccagacaaa attcagactt aacatcgggt aggcaatgcc taccggtacg ttctgcaaga
     7321 ccagcagcca gagcatcatg gcaagaccaa gacaagccag cgccagtccc agccacagca
     7381 cgatatgttt gcgccgtttg tttatcgcca caaagcaggt tgcctgtttc tgacacaact
     7441 gcccggcaac gctaagcaag ctggcaaaga ctaatgttag ccagatcatt tgggacgata
     7501 ctgaatcagc accagacgct cctgacgaac gatggcatct gcgggcggaa tggcgagact
     7561 gttgatatct tcatcgcggt caaccgaaag cacgagtgta ataatcccct cctgacgatg
     7621 ttgattaagc cagtttgcga actcatcacc gctgacaaat ctccctttcg catccggata
     7681 attaaggccg tatttcaact cacctgtctg gcgatacatg atgatgtcat cgcgttgcag
     7741 gctccatgcc agacctgcgg caacaccgac gctatcagta agaatatagc ggcttggctg
     7801 aagtgattct tgtgtcatct cgacaaaaaa ttgcggatgt tttccttcca tgactctgtc
     7861 aggaattgaa aatcctacca gcaacgccag ccccagcggg caaagcgcgg cgaaagacca
     7921 ggtcttttcg acgtttgtta aggtgtacca gccgaaaaat gcccatagcg aaaaaatcga
     7981 ccaggcacaa aagactttat agctctcgaa ggtttgccac accggcgtgt tcattggtcc
     8041 ccagggggag atcacaaatg tggcaataat gccagtgacg ccaaaagcga tgttaatcca
     8101 gccattaatc cgcagcgcca gaggattatt ttttgctgcc agcaacgcgt aatgcgccaa
     8161 cagcattgcc agaggtgcaa agcaagaaag aatataggtg ggcaatttgc ctttagcgac
     8221 ggagaaaaac agcagcggca ttatcgtcca gctcaacaaa tagacggttg cggaatgctt
     8281 gcggtttttc cagcctgtgt acagtgcacc ggggagtaat cccagccacg gcaggctacc
     8341 ggcaatgatg accggcacgt agtaccagaa cggagctcta tgttgggcat catctagtgc
     8401 aaagcgttga atatgctcaa cccagaaaaa atagtgccag aagtcaggct cccgctgcgc
     8461 tatcgccagt ccccagggga gaaccgtcag tacgcaactg ataaccgcca gccagccgta
     8521 aataaagaga tctttccagc gtttttgcgt cgctacccat ggcagcacgc ttaataccgg
     8581 cacggcaagg gcgagaaaac ctttggtcat cacccccatg ccgcaggtga ttcccagcag
     8641 taaaaatcct gcgcttttgc ctttccacgt ctgtgcctgc attgccagcc agaagctgca
     8701 cattcccgcc accagccaga atgcaataaa cggatcgagc acggcataag tgccgatggc
     8761 atagacaata aacaatgaga gataaattac tgtggcgagt agagccagac gtttatcgcg
     8821 ccataagcgc agagtaaacc aggtcaccag cgcggcagtt aacagggtcg caaagataac
     8881 gcctgcccgc acaccaaagt tattcgcgcc aaatagccat tgcccaatgc tgttaatcca
     8941 gtatccggca atgggttttt cgaaataacg tagccctaac agatggggca caatccagtc
     9001 gccggatgcc agcatttctc gactgatttc cgcataacgc gtttcatcgg gttgccagag
     9061 cagacgcgtg ctgatcggta acaggtaata gcaggcaata aacgcgaaga tgccgataag
     9121 gtaacgtacc gatttcatca gcgactaccc gcaatttgtt ggcaacccag ccagccttca
     9181 cgtccggcaa tatttccgcg aacaacttgt ccgagcggca acgtctctga taacagttcg
     9241 cttaaagggc aaaatgtcac gccttcctga gctgcgcgtt tgaggagatc cacaaaatta
     9301 tgctgataag cgcagccttc aacttctgca tgaatggtat aaaccggcgt gcctttatct
     9361 cgcaggatgc ggttgagtaa ccaaccgtta aaatcttctg ctttcacatc ccgaccaatc
     9421 acttcatccc aggtgggtaa ggtcaccgga atttgcgcag tgccagggtt tcctgattca
     9481 agcagtggac ggaacggtat ggccccacga caatcgctgt tgtagcgcaa atggaacgct
     9541 tcttttgctt cgatcacctt ctggtcggca cgccagcccg cagcggcaga acaggttacc
     9601 ggttgaccga taatctcttc cagagtgcga agaccgcgtg caatatcgtc gatcattgtt
     9661 tgccgatccc agttaccgct acgggcttgc caggcatggt gatcccaggc gtgcagaccg
     9721 acttcgtgat gtttagccgc ttcacgaatg atatcggcat tggcatgacc aatctcttta
     9781 cctggccagg ccgtacctgc cagtaaaata tcccagccat aaagcgatgc cgcgtttgag
     9841 cgcagcatct tccacaaaaa ctgtggcttc accagtcgcc agagatggcg gcccatattg
     9901 tccgggccga cgctgaaaaa aatgctggcc tgaatattat gcttactcaa gatttccagc
     9961 agacgcggca cgccttcacg ggtgccacga aaggcatcga catcaatgcg taagcctact
    10021 ttggtcatga tggtttatcc gtaagatcaa cggtgcgcag gaagaaatcc agcgtttcgt
    10081 cgatggtttc ctgcatatca attttcggct cccagtccag gcagcggtgg gcattgcgga
    10141 tgctcggttt acgatgctct acgtcctgat atcctttgcc gtagtagcag ctactttcga
    10201 caacgcgaaa gcccgcaaac ggtgggaaat gatggcgcag cggatgtttt tcgaagctcg
    10261 ccagcagcat ctcgcccagt tcctcaatgc tcgcttcgtt ctcaggattg ccaatgttga
    10321 taatttcgcc gtcgcagcga tttcccgcat tttcgataat gtggtacaac gcctcgatac
    10381 cgtcccggat atcagtaaag cagcgttttt gttttccgcc atcaattagc ttaattggtg
    10441 aaccttctac cagattgaga atgagttgcg taatagcgcg ggagctgcca attcgcgctg
    10501 cattaaggtt atccagtcgt ggtcccatcc agttaaacgg gaggaagagg gtgaactgta
    10561 aaccctcttt ttcgccatag gcccagatca cccgatcaag taattgtttt gacaccgaat
    10621 aaatccagcg tggtttattc accgggccga cgattaaatt agaatggtcc tcatcgaagt
    10681 atttatcgct acacatccca taaacttctg aagttgacgg gaagatgatt cgcttacggt
    10741 acttaacgca gtagcggata atgcgcagat tctcttcaaa atcgagttca aatacgcgca
    10801 gcgggttgcg ggtatattca atcggcgtgg ctatcgccac cagcggcaag acgacatcac
    10861 attttttgac gtgatactca atccattcgg aatgaatact gatatcgcct tcgacaaagt
    10921 gaaaatgtgg atgattcaga aaacggctta tcgcatcgct gccaatatcc agaccgtaaa
    10981 cttcataatg atcttcgcgc agcaggcgtt ctgtcagatg gttgccaata aagccattca
    11041 ccccgaggat gagtacccgg gtacggcgtc gggcggtgca ggcaggctgg ctattcaagc
    11101 gtgaaccttg caccaggccc agcatctgcg ctaattgcga gccctgcata gtaatgccgt
    11161 cgcccgcctg tccggtgacg atttccagcg cgccatcgcc acaggcaatc agcagtggcg
    11221 caacagaaat cacgctcccc ggctgtgctt tgctggcatg aggatgaaca cgcgacgacc
    11281 agacggtgaa tttctgattg ccaacatagc tgaaggcacc cggccacgga tcggcaacgg
    11341 cacgtaccat gttgtgcagt acggatgccg gtttatgcca ttcaaggaaa ctgtcatccg
    11401 gcgttctgcg accaaaacag gtggcttcgt tttcgcgctg ggcgatttcc agaatattac
    11461 cgtgtttaat ggcgggtaat gtctgttcca gtagctggcg cgcggcatgg cacaatttat
    11521 gatgcagcgt aatagcgata tcgtctggcg caatggcaac gcgcagttgc gccacaatgg
    11581 ccccagcatc ggcacgttta ccattcggtg caatgtaacg cccgtttccg tttcaccgtt
    11641 caccagcacc cagttcagcg gcgcgcgacc acgatatttt ggtaacagcg aaccgtgcag
    11701 attaaatgca cctgcgggag cgagctgaaa aatctcgtcg caaataagat ggcgataata
    11761 aaaagagaaa atcacctctg gtgacagttg ggcaatgcgt tccacccaca gcggatgatt
    11821 aacgttatcc ggcgcataaa ccggaatgtc tctttccgcc gccagatgag ccaccgaacc
    11881 ataaaaggct ttttcaccgg gattatcggt atgggtaaaa atggcgctaa tttcgtaacc
    11941 ggcagccagc agagcttcaa taccgaggca tcccatatcg tggtaggcaa aaacgacggt
    12001 tttcattatt cattttcctt gctggatgga cggataactt gctgaacaaa atagcggggg
    12061 cgggcgcgga catcggtgta gatcctgccg atatattcac cgagtaatcc catgccgata
    12121 aactgagcgc caataaaagt aaacagcacg gcaaatagca taaagacgcc ttctgccgcc
    12181 cattgtggtc cgaaggtcag gcgtaaaatc accagcagca ccgcaatgct aaaacctcca
    12241 atcgcaataa tgctgccgag cagactcagc atacgtagcg gcgttgtggt aaggcaggtc
    12301 accaggtcgt acatcaaatt aatcaggcgc ataaaactgt atttggattc accaaactca
    12361 cgctcggcat gatgtactgg aatttcaatt gcacggcggg cgaagatatt cgccagaatc
    12421 gggataaagg tgctgcgttc atggcagtgc aacatcgcat cgacaatatg acggcgatag
    12481 gcgcgcagca tacaaccgta gtcacccatc gctttgccag tggtgcgctg aataagccgg
    12541 ttaatcatct tcgaagctgt tttacgaaac cagctgtcct ggcggttctg gcgtacagtc
    12601 cctaccacgt cgtaaccttc atcggctttt gccaccaggc gggggatttc ttctggcgga
    12661 ttctggagat cggcatcaag ggtaataatt aagtcgccag taacgtgact gaatcccgcc
    12721 ataatcgctg aatgttgccc gtaattgcgg ttaatcaaaa tagacacaat atggctgttc
    12781 tccgcttgtg aggcttcgac cagcatatgc gcggaattat cgctactgcc gtcatcaatc
    12841 agcaggatct catactcttt ccccaacgat tcacaggctg tggtggtgcg cctgattaat
    12901 tccggtaagc tttcctgctc gttataaacg ggaataacca ccgagacttt cttaacaggg
    12961 tggatttcaa acatggctta ttgtcctgcg agttgctgaa gggctgtgat gacgcggtcg
    13021 gcatcggcgg tagtcatatc cgggaacagc ggcaacgaac agatgcgttc gctattccat
    13081 tcggtattcg gtaacgacag cgtgggaaaa cgctcgcgat aatatttttg tgtgtgagcg
    13141 gcgcggaaat gtaacccggt accaatgcct ctttctttta acgcttccat caacgcatcg
    13201 cgactgatac cacaacgttg ttcatcgaca cgaataataa acagatgcca ggcgtgaacg
    13261 tgcggccagg cgggaaggct taatggctga aaggggagag ctgccagtgc ttgctgatat
    13321 tgctgggcaa tttcgcgccg acgggtgttg aggtgctcta atttgactaa ctgtgtcagg
    13381 gcaatcgcgg cgttaatatc ggtcagattg tacttatagc ccggtgttaa gacttcagcc
    13441 tgcggtgcac ggccccaggt ttgtctgtca taggcatcga cacccagacc gtgaaatttc
    13501 agcatccgta gctggcgggc aaggttttca ttatcagtta caatcaggcc accttcagca
    13561 caggtaatat ttttaatggc atgaaatgaa aaaatagcgg taccttttgc gccaatatgt
    13621 cgccctttgt aatacgtacc gacggcatgg gcagcatctt cgataactgc gatgccgtaa
    13681 cgttcgccaa tggcgcgaat ggcgtcaata tctgctggcg caccggcata atgcaccgga
    13741 atgatggctt tagtgcgtgg cgtaatggct gattcgatcg cttcaggcgt gaccatcagc
    13801 gtatcgcggt cgacatccac cattaccggc gttgcaccca gcaaggaaat catattgagg
    13861 gttgaaaccc aggtcaggga aggcgtaatc acttcatcgc ccttgccaat ttccaacgcc
    13921 attagcgtga tatgcattcc ggcggtggct gaactgaccg cgatggcatg ctgatttccc
    13981 gtcaactggc aaaaggcttg ctcaagcgcc tgattcttcg gaccggttgt gatccaaccg
    14041 gattcgagaa cctctttaac tgcagcgagt tcctccacgc ccattgctgg tcgcgaaaaa
    14101 ggcaaaaatt ctgacattgc ttttccttcc gccattgaat acctgtccac ttatatttgc
    14161 tataaagagt gttgtgtata ttttgccatt tggagcgaaa ttttaaggat agaatattaa
    14221 cttaacctta agaaactaat attagacgta aatattgaaa tttttatatt ttttcttatt
    14281 taggctttgc atttggcaaa aatttgaggc attttgccga catcgtagga tttttaatat
    14341 tacaccaact gctaattatc gccagaaatg tcgctcaatt tcacggtaat tgtctggttg
    14401 cgcttgtcta taggtggagt ttacgtgtta gctttttgcc gctcatcatt gaagtcaaaa
    14461 aaatatttca tcattttact ggcgctcgct gcaattgccg gactgggtac tcatgccgcc
    14521 tggagtagca atggtttgcc acgtatcgac aataaaacat tggccagact ggcacagcag
    14581 cacccggttg tcgttttgtt tcgccatgct gaacgttgcg atcgttcaac caatcaatgc
    14641 ttgtcagata aaacaggtat tacggttaaa ggtacccagg atgcccgtga actgggcaac
    14701 gcttttagcg ctgacatctc tgatttcgat ctttattcca gtaataccgt ccggaccatt
    14761 cagtcggcga cctggttttc cgcgggtaaa aagctgacgg tagataaacg acttcttcag
    14821 tgcggtaatg agatttatag tgcaattaag gacttacaaa gcaaagcgcc tgataaaaat
    14881 atcgttattt tcacccataa tcattgcctg acatatattg ctaaaaataa gcgtgatgcg
    14941 atatttaaac ctgattatct ggatggttta gtcatgcatg tggaaaaagg caaagtttat
    15001 ctggatgggg aattcgttaa ccactaaatt ttcaatctga cagccagtga tggctgtcat
    15061 cattgttaaa gaagaccttt caaacgtaac gtttttcggg tggcaacatt taaatcataa
    15121 tgcaccagat cttcaggttt tacccacgcg tagtcctgaa actcttcgtt tattttcact
    15181 tctcggttgg cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga
    15241 ccatctgcat atgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc
    15301 aaaagcagct gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt
    15361 tcgccagact ccacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg
    15421 tcggccattt tgcacagcaa ataagcacca tcattttgaa tcaaagggca tacaatagtc
    15481 cgttgtcgca caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca
    15541 gaaatatgtg aaagtgacag taatctcttt tgcaatgata actgcactta ttattatttt
    15601 tgtgtgtatc ggtaaagtgt gtttattttt tcggcgttta attatttaag aagtatttag
    15661 ttacaataag tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca
    15721 atcccgcagc tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat
    15781 tgcgctagca ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat
    15841 cagcggccag gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac
    15901 gggcctggct ttaagcggta actggacaca taacgacgac gacggtgacg tcgcgggcgt
    15961 ggggctgggg ttgaatctgc ctctcgggcc gttaatggcg accgttggcg gaaaaggcgt
    16021 gtacaccaac ccgaattacg gcgatgaagg ttatgccgca gcggtaggag gtggtttgca
    16081 gtggaaaatt ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcgct
    16141 ctccagcggt attcaaagtt atgaggaagc gaatgctggc gcgcgttaca ccattatgcg
    16201 tccagtcagt attgaggcgg gttatcgcta cctgaatctg tcgggtaaag acggtaaccg
    16261 cgacaacgct gtggctgacg gcctgtatgt tggggttaac gccagtttct gatcccttcc
    16321 ggcgcggcat tcagtttctt actacgcctt atctcccttt gcgttatagt gattcgataa
    16381 caaaagcagg agataaccat gttaaaagtg gaaatgctat ccaccgggga tgaagtgtta
    16441 tacgggcaaa tcgttgacac taacgctgcc tggctggccg attttttctt tcatcagggg
    16501 ctgccattat ctcgccgcaa tacggtgggg gataaccttg atgacttagt caccattctt
    16561 cgcgaacgta gtcagcacgc cgatgtgctg atcgttaacg gcgggctggg accgaccagc
    16621 gatgatttaa gcgcactcgc cgctgcgaca gcaaaaggtg aaggcctggt gctgcatgaa
    16681 gcctggctca aagagatgga acgctatttc cacgaacgtg gacgagtaat ggcaccgagc
    16741 aaccgtaaac aagcggagct gcctgccagt gctgaattta tcaataaccc ggtaggcacc
    16801 gcctgtggtt ttgccgtgca gcttaatcgt tgcctgatgt tctttactcc cggcgtaccg
    16861 tcagaattta aggtgatggt cgagcacgaa atcctgccgc gcctgcgcga gcgtttttct
    16921 ttaccacagc cgccggtttg tctgcgttta actacctttg gtcgttcgga aagcgatctg
    16981 gcacaaagcc tggacactct acaactgccg ccgggcgtaa caatgggcta tcgctcctca
    17041 atgccgatca tcgaactgaa actcaccgga ccggcaagcg agcaacaggc gatggaaaaa
    17101 ctgtggctgg acgttaaacg tgttgccgga cagagcgtga ttttcgaagg caccgaagga
    17161 ctgcccgcgc agatcagtcg cgaattgcaa aaccgccagt tcagcctgac gttgagcgag
    17221 caattcaccg gtggtttatt ggctttgcaa ctttctcgcg caggtgctcc attgctggcg
    17281 tgtgaagtga ttccttcaca ggaagaaacc ctggcgcaaa ctgcgcactg gattacagaa
    17341 cggcgggcca accattttgc cgggctggca ctggctgttt cgggtttcga gaacgagcat
    17401 ctcaactttg cgctagccac gccagacggc actttcgctc tgcgtgtgcg tttcagcact
    17461 acacgctaca gcctggctat ccgtcaggaa gtgtgcgcaa tgatggcact gaatatgcta
    17521 cgccgttggt taaacggcca ggacatcgct agtgagcatg gctggattga ggttgttgag
    17581 tccatgactt tatccgtctg atttcgccac tctgtgccgg tttactgcac cggcacattc
    17641 cccctccggc aaaatttgtc caaccctccc tacggtgagc gtggtgttgg ggccagtgtg
    17701 gcacgggctg cgcgctgggg acgcattgag aattacatgg cgcaagtcaa cgattcgctt
    17761 tgtctgttag tgcaggtgga aagtaaaacg gcactggata acctggacga gatcctcgac
    17821 gtcgaaggga ttgatggcgt gtttattgga ccagcggatc tttctgcgtc gttgggctac
    17881 ccggataacg ccgggcaccc ggaagtgcag cgaattattg aaaccagcat tcggcggatc
    17941 cgtgctgcgg gtaaagcggc tggttttctg gctgtggctc ctgatatggc gcagcaatgc
    18001 ctggcgtggg gagcgaactt tgtcgctgtt ggcgttgaca cgatgctcta cagcgatgcc
    18061 ctggatcaac gactggcgat gtttaaatca ggcaaaaatg ggccacgcat aaaaggtagt
    18121 tattgatatc aaaggcccat ggggatcggc tgtgggcctg tgttaattag tggttattcg
    18181 ctgccagatc ggcttcgctt agctgggtgg ccgcgagcac ttggtcacga tcaatgccct
    18241 gttccagcat gcgcaaagca attttaatgg cttgttcatg cataccttcc tgcaaacctt
    18301 ctaatttacc ttgctgcaaa cctactaatt taccttcttg ccagccaatt tgatgtcctt
    18361 cctgccgtag ccgttcagca atagtcatta atctctcctt ttgtagtggt gaacgttcgg
    18421 caatctcctg aataaaacgg gtgaaacggg aggtatcgcc gcattgcagc agataattaa
    18481 acagtgtttg tagctggctg tcattagtga agcctctaac caaaagcgtg gtgatcctgt
    18541 cgaccatgcc gattaaatcg tggtcgcgaa tatgcttttg aatcagttcc agcagagcta
    18601 tacgccgatg ttgcatgatc tcatcgtcag gtacgatggt gatatccacc aacggaaaag
    18661 cttcggtgta caactgccgg gcaagttgcg gatcgtcaaa ctcatccagc cagttaagtg
    18721 agtacgggta gggcgatgtt tcgccatgat aaaacagcaa tggcaccacc agcggaactc
    18781 tgtcatagcc tttatctagg tgactctgca tggcggcagt ggcatagcgc attggccgaa
    18841 aagccatatt cttttctgca gagctttgat gttcaatcac gcagtagata tagccgtcac
    18901 cgtcgctggt ttccacggac cacaaaacat ccgagtagta agcgcgtaaa cttttttcaa
    18961 taaaactagt gggttccagg cgtaaggttt gcaggttgca aagcttgcgc agtggttctg
    19021 gtaaatgtat ttcgagaaaa tcccgtgcgg tttcgggtgt gaatataaag gttttaaata
    19081 ccgcatcatg cggggaggag gttgttgatt cggtcatggt tgtccgtcac ctgatattta
    19141 tgatgacgcg acattacggt tatcccaggc ctttgacatc tgccagtttc agcctttgcg
    19201 agacgttttc agaggaattt tcactgcgtt gcaacgcata cattgggcac ggaatcgtgt
    19261 acgcaaaaat gcccggtaac cacatgctta ccgggcatca gaagggagtt taagccagcg
    19321 cctgggccag tagcgtaatc ggatgttcgc agcgaagact tgtggacatc tcaatctgcc
    19381 atttacaggt ttcgcagtcg gtgaccacca gatctgcgcc gctttcttct atctggcgga
    19441 acagtggtgc gccgatggct tgtgaggtgg ggtagttctc ttttttgaaa ccgtaagtac
    19501 ccgcaatacc gcagcactgg gaatccagca ccgttaactc aagccccggg atgttacgca
    19561 acagctccag ggtgtagagc gtccagccca ttttttccat atggcacgga gtgtgataaa
    19621 ccactttcag cggcagcggt ttcagcggta acgttttgcc ttcgtccagc ttgcgccaca
    19681 gccagcgggt tgccagttcg atatgatcgc gcaagccttt gttgtcgaca ttcagcactt
    19741 ccgggtattc gtcgcgcagg gcaaatgtac aggttgagga ggtggcaatc accggaatgc
    19801 cttttactcc cacagcttcg cggatcgact ctacattcgt aattgcctgt ttgcgtgctt
    19861 tatcggtaaa gccgttggcg attagcggta cgccgcagca tttttctttg ctgagcagtt
    19921 gtacaccggt acccattgcg ttgagcactt taattaaatc tttacctaac tgcggatggt
    19981 tgtagttaac gaagcagccg tgaaagaaag cgacctggtc tttatattgt gcttgctgag
    20041 ccgctatgct gcgataccag cgacggaacg tgccgaagga gtatttcggt agcgtgcgac
    20101 gatgatcgat ttttaacgcc gcatcaagca gctgccgcac cggtttcagc gaggtagcgg
    20161 tgttgacgat tggtgcgaac ggcgtggaaa cgctacccat caggtcggta tgactcaaca
    20221 caaaattacg cagcgacggg cgcgtggtgt catatttcgc ccgcgcgcgc tggataatat
    20281 cgccaatctt cacatcggac gggcaggcga cttcacaacg tttgcagttg atgcaatatt
    20341 tcagcgcctc gtcatacagt gcgccatctt tcaaacgcag acgctcgcca tccggcccgg
    20401 cttgttttgg ccctggataa ccgggattca cccggctcac cgggcaggcg gtggtgcaga
    20461 cggtgcactt aatgcagttt tcgaagctgg tgtcgttcat tgttggcctc ctgcgcgttg
    20521 ggcaatctgt tgagcggcat gtaaagcact gacggcacaa acaccgccgc cgcatccctg
    20581 ggcgatggga tcaaatccgc ccagcaccga accgatggca aacaggttct caatggtttg
    20641 ccctgcctgc gacgggcgta gcgtctcatc agtggttaca ccgaactgct gccacggttg
    20701 cggcgcaaaa aaatcgccct tataccattc accccgcgtg gcggtttgta gcacatcaag
    20761 gccgagaatc ggctcgcgaa tgccgttacg ttccgctact agtccgccac taaagaaact
    20821 gccgctggcg agaaccgcga aacgtggacg tagcggaata tcggcgtgat tgcgggtcca
    20881 gatttcattc actacgccat ttttacaggt cacttttttc acttcatcgc ccggcatcca
    20941 cactccacct tggcgcacaa actggcgctg taactggttt tgcagacgaa tgcccagcac
    21001 ggaaggcggc agcgttggca aaagcatcag tgaacaaggt agtttttcat tcaaccaacg
    21061 ccacagtttg tcatcggcca ggccgaagca ggcgggcatc aggatcattt cgcaggtatt
    21121 ggcgacagga ataagcgcat caagtaacag cggccagttt tcttcattat caaggaaacg
    21181 ggcgatattc accgcgcgaa attcggtggc gttattgcgc aggacatcca gttccggcag
    21241 ctctatttct gcggtttcaa ccgaaagatc cagttcgcgc aacgaagccg ccgcaagatg
    21301 cgcctggaaa tccatcagcc cgcttatccc cactacacat atcttcttcg cgggtagcgg
    21361 ccagacggga acttctggcg aacttaacca ggttgcacgc aaagtgccga gcggcgtaat
    21421 ccgctggtga gccagttcta cgctgccctg caattgcgca ccgctttcag cgattaatgc
    21481 ctgcgcctgg caagcgagat cgagcacgcg ttgtggcccg agaagagagt aaggatgtgc
    21541 aggtgcctgc tgacgcaaag attccagtcc actgtgaatg tctgccacgg gttgaccatc
    21601 tggcagatgg ctcagcaaat ccagcgaacc ggatgagaaa tgcaacgcgc tttgaccacg
    21661 cgtgacaatg gcacagcgca ggccgtgttt ttgcagttgc aggccacaga gtaatccggc
    21721 gaggccgccg cccataatga cagtatcaaa gcgcatcttt ctgctccttc tccagaccac
    21781 acagtccctg ataaacccag cgggtaaatt cgctttcgcg cagtgcgtct ccccaggcga
    21841 tgggttgcac gcctttccag cgttcgttaa ggaaggtgga aagttgctcg atagattgcg
    21901 cggacgtcgt gacgttaaac cgttgcagca gtccggcagc gcggcaggcg cagagttcgc
    21961 cctggcaggt gcccatcccc acacgggtac gacggcgtaa atccagcagg ctattaacgt
    22021 ttaaattttc taccgcgtac tgcacttcac ccgcagttac cgcttcgcac tcacatacca
    22081 ggctacggtg tagacggcct tcgctcagcc aggcaggcgt gcgatcgcca tgacgataaa
    22141 ccgcagaacc gcgcagcggg gcaggcaggg agatgacttt acgcaaggta acttcagcgg
    22201 gttcttgtga accaggcagt gccagatcgg cagtcgtaca ggggcgcgtg ttgcccagtt
    22261 tgcggcatac cgcgtcggta gcccattcag ccatcagccg ataggtcatc agtttgccac
    22321 cggtgatggt gataaatccg tccagaccat cgcgttcagc atggtcgagc agcacgatgc
    22381 cacggctgac gttacgtccg ctcgggtcgt catcgctggc aaccagcggg cgcacgccag
    22441 aataggcccg caaaatgcgc gttttcgcca tcacgggggc cagtttttcc ccttcacgca
    22501 gcagaatatc aacctcttct gccgtcactc gattatcgtc aatctcgttg taatcaatac
    22561 gtaaagaggt ggtaccaatc agcgaaatgg tatcgccagg caccagaata tcggcgtcgg
    22621 aaggtttacg gcagcggttg atcacatgct ggttaatgcg gtgatccatg atcagcagcg
    22681 atcctttcgc cgggaacatg cgaatgcgca gatcggcata ttcggcaatg tgttgccccc
    22741 agatcccagc ggcattaacc acgacaggtg catgaagggc ctgagtttcg ccggtgagat
    22801 ggttacgtac acgaacaccg cacaccgtcg cgccttcacg aatcagcccc gtgacttcat
    22861 gagcggtaag gataacggca ccgtgttctt tggcatccag catgtttgct gcggtcagac
    22921 gaaatggatc aacggtgcca tccggaactt tcaccgcgcc aatcagtgcc gggttaacgg
    22981 caggttcgat aatgcgcgct tgctgcgggt ctatagcttc tgcgctgatc cctgcttctt
    23041 cgcaggcgcg aataaaagtg gcctggaatg agaggtcatc ttccggcagg gtgataaaca
    23101 ggccgttggt tggttcaacg cagtgacgtg caatgcgttt caggatctgg ttttcactaa
    23161 tgcattcgcg ggccgattcc gcatcggtta ctgcatagcg cgcaccgctg tgcagcaggc
    23221 cgtggttacg cccggtggca ccggttgcga tgtcgtggcg ctcaaccaaa atcacgcgca
    23281 gcccgcgcag ggcacagtcg cgggcaatcc cggctcccgt tgcgccgccg ccaatgataa
    23341 tcacgtcact tgattgcgag tcgcgagttt tcattgttat ccctctgaag ttcgtttttt
    23401 accatttagc catagtaaaa acatgaattg tttgatttcg cgcatattcg ctcatgattc
    23461 gaaagtaaaa cgtgatttca tgcgtcattt tgaacatttt gtaaatctta tttaataatg
    23521 tgtgcggcaa ttcacattta atttatgaat gttttcttaa catcgcggca actcaagaaa
    23581 cggcaggttc tctcactgaa tcaggctgtt aatcataaat aagaccacgg gccacggagg
    23641 ctatcaatgt tgagtatttt taaaccagcg ccacacaaag cgcgcttacc tgccgcggag
    23701 atcgatccga cttatcgtcg attgcgctgg caaattttcc tggggatatt ctttggctat
    23761 gcggcttact atttggttcg taagaacttt gcgcttgcta tgccttatct ggttgagcag
    23821 ggattctcac gcggtgattt aggttttgcc ctttcgggga tctcgattgc ttatggattt
    23881 tcgaaattca tcatgggttc ggtatcggat cgctcgaatc cgcgcgtttt cctgcccgca
    23941 ggtttgattc tggcggcggc agtgatgttg tttatgggct ttgtgccatg ggcgacgtcg
    24001 agcattgcgg tgatgtttgt actgttgttc ctctgcggtt ggttccaggg gatggggtgg
    24061 ccgccgtgtg gtcgtactat ggtgcactgg tggtcgcaga aagaacgtgg cggcattgtg
    24121 tcagtgtgga actgtgcgca caacgtcggt ggtggtattc cgccgctgct gttcctgctg
    24181 gggatggcct ggttcaatga ctggcatgcg gcgctctata tgcctgcttt ctgcgccatt
    24241 ctggtggcat tattcgcctt tgcgatgatg cgcgataccc cgcaatcctg tggcttgccg
    24301 ccgatcgaag agtacaaaaa tgattatccg gacgactata acgaaaaagc ggaacaggag
    24361 ctgaccgcga agcaaatctt catgcagtac gtgctgccga acaaactgct gtggtacatc
    24421 gccatcgcca acgtgttcgt ttatctgctg cgttacggca tcctcgactg gtcaccgact
    24481 tatctgaaag aggttaagca tttcgcgctg gataaatcct cctgggccta cttcttctat
    24541 gagtatgcgg gtattccggg caccctgctg tgcggctgga tgtcggacaa agtcttccgt
    24601 ggtaaccgtg gggcaaccgg cgtcttcttt atgacactgg tgaccatcgc gactatcgtt
    24661 tactggatga acccggcagg taacccaacc gtcgatatga tttgtatgat tgttatcggc
    24721 ttcctgatct acggtcctgt gatgctgatc ggtctgcatg cgctggaact ggcaccgaaa
    24781 aaagcggcag gtacggcagc gggctttacc gggctgtttg gttacctggg cggttcggtg
    24841 gcggcgagcg cgattgttgg ctacaccgtg gacttcttcg gctgggatgg cggctttatg
    24901 gtaatgattg gcggcagcat tctggcggtt atcttgttga ttgttgtgat gattggcgaa
    24961 aaacgtcgcc atgaacaatt actgcaaaaa cgcaacggag gctaatggca tgaaattgaa
    25021 gctgaaaaac cttagcatgg cgatcatgat gagcactata gtcatgggaa gcagtgcaat
    25081 ggcggcggac agcaacgaaa aaatagtcat cgcccatcgc ggtgccagtg gatatttgcc
    25141 ggagcatacg ctgccagcaa aagcgatggc gtatgcgcag ggagcggatt atctggaaca
    25201 ggatttggtg atgaccaaag acgaccatct ggttgttctg catgaccatt atctcgatcg
    25261 tgttactgat gttgccgatc gtttcccgga tcgggcgcgc aaagacggtc gttactacgc
    25321 gatagatttc acgctggatg aaattaagtc gctgaaattt accgaaggtt tcgatattga
    25381 aaacggtaaa aaagtacaga cttatccggg gcgtttccca atgggtaagt ccgacttccg
    25441 ggtgcacacc tttgaagaag agattgaatt tgttcagggg ttaaatcact ctaccgggaa
    25501 aaatatcggt atctatccag aaatcaaagc gccgtggttc catcatcagg aagggaagga
    25561 tattgcggca aaaacgctgg aagtgctgaa gaaatatggt tacaccggta aagacgataa
    25621 agtttatttg caatgttttg atgctgatga gctgaagcgt attaagaatg agctggaacc
    25681 caaaatgggc atggagctca atctggtaca gctgattgcc tataccgact ggaatgaaac
    25741 gcagcagaaa cagccggatg gaagctgggt taattacaac tacgactgga tgtttaagcc
    25801 gggagccatg aaacaggtgg cggaatatgc agatggtatt ggtccggatt accatatgtt
    25861 gattgaggag acatcgcagc cgggtaatat caaactcact ggcatggtgc aagatgccca
    25921 gcagaataaa ctggtagtgc atccttatac cgtgcggtca gataaactgc ctgaatacac
    25981 tcctgatgtg aatcagttat atgatgctct gtataacaaa gcgggtgtaa atgggctgtt
    26041 tactgatttc cctgataagg cagtaaaatt tcttaataaa gagtaatgtt actgcaggat
    26101 ggcgttcatg cgccatcctg tctcatatca aatttttcca atcgccacta attgttccat
    26161 gcacatttgt agtaaaaaca gtggactgcc ttctaccggc ttttctttag tcagcaacga
    26221 aatctgtcga tagaaggttg gttcgtgagg aacggaacac aattcacctg caatgctttt
    26281 cagcgttaac tctggttgta aggcgatgcc taatccctgt cgaataaaat tcatcgctgt
    26341 atccgactga ttaaattcac attttatctg ctgggcgcac tattagtggt agtggctggt
    26401 ggaatctatg cgctgcaaat gaagggcaaa agcggtgtca cacctgtagc ggcaaaagaa
    26461 attcattaat acgacacgtt tcattaagat tttcctcagg taaccaccta tagtcattgt
    26521 cgttaataaa aatgacaggc gatagggtat ggcagtttca gcaaagtacg acgaatttaa
    26581 tcactggtgg gcaacagagg gcgactgggt tgaagagcct aactatcgcc gtaatggtat
    26641 gagcggggtg caatgcgtcg agcgcaacgg caaaaagctg tatgtaaagc gcatgacgca
    26701 tcatctgttt cattcagtac gttatccgtt cggccgacca acgattgtcc gtgaggttgc
    26761 ggtaattaaa gaactggaac gggccggtgt catcgtgccg aagatcgttt ttggtgaagc
    26821 ggtgaaaatt gagggtgaat ggcgcgcgtt gctggtgact gaagatatgg cggggttcat
    26881 cagcattgct gactggtatg cccggcatgc agtatcgcct tattctgacg aagtacggca
    26941 agccatgttg aaagcagtgg cactggcgtt taagaaaatg catagcgtta atcgtcagca
    27001 tggctgttgc tatgttcgcc atatctatgt gaaaacagaa ggtaaagcag aagctggttt
    27061 tcttgatctg gagaaaagtc gtcgccgctt gcgtcgcgat aaagcgatta atcatgactt
    27121 ccgccagctg gaaaaatatc tggagccgat ccctaaagcg gactgggagc aggtgaaagc
    27181 gtattactac gccatgtaat cgttgtaggc cagataatac gcgtaagcgt cgtatctggc
    27241 gctagtaccc gatcacaact cgatttcaat atcgcctttt gcccggcaac aacagggcaa
    27301 aatttccccc ggctgaataa aggctaacgg ttcggcaatc cagtccacct gacctgcgac
    27361 cagtcgggtg cgacaggagc cgcagtaacc ttcgcggcac tggtactcaa ccgccacatt
    27421 gtgggattcc agcgccgcca gaagggaagg gtgttcatcc tggcacagca gttgtgtgcc
    27481 agtgatgcgc agggtaacgc gggccatcag agctggaagt tactcaaatc gtcggtgtcc
    27541 acttccgcgt caatctgccc gaccagataa gaactgactt ccacttcctg cggagcaacc
    27601 tgcacgttat cagacaccag ccaggtgttg atccacggga tcgggttgga gcgcgtctgg
    27661 aacggcagat ccaaaccgac tgcctgcatg cggatattgg tgatgtattc aacgtactgg
    27721 cagagaatat ctttattcag accaatcatc gaaccgtcac ggaacagata atccgcccag
    27781 tctttctctt gctgtgccgc ctggacaaac aggtcatagc actcctgctt acattcttcg
    27841 gcaatttccg ccatctccgg atcatccgcg ccgctgcgca gcagattcag catatgctga
    27901 gtaccggtca ggtgcagggc ttcatcgcgg gcaatcaggc gaataatctt ggcgttacct
    27961 tccatcagtt cgcgttctgc aaaggcaaag gaacaagcaa agctgacgta gaagcggatc
    28021 gcttccagcg cgttaacgct catcaggcag agatacagtt ttttcttcag ctcgcgcagg
    28081 ctaacggtta cggttttacc gttaacggtg tgggtgcctt cgcccagaag gtgccagtag
    28141 ctggtcattt cgatcagttc atcgtaatag ctggagatcc cttccgcacg tttctggatc
    28201 tgctcgttgg tgacgatatc gtcaaacaca acagacggat cgttaacgat attacgaatg
    28261 atatgagtat aggaacggga atgaatcgtt tctgagaacg cccaggtttc gacccaggtt
    28321 tccagttccg gaatagaaat aagcggcaat agcgccacgt tcgggctacg accctgaatg
    28381 gaatccagca gcgtctgata tttcaggttg ctgataaaga tgtgtttttc gtgctccggc
    28441 agcgcctggt aatctatacg gtcgcgggag acgtcaactt cttccggacg ccagaagaaa
    28501 gagagctgct tttcgatcag cttttcgaag atgtcatatt tttgctgatc gtagcgagcc
    28561 acgttgaccg gctgaccaaa gaacatcggt tctttgagct gatcattttt cgtctgtgaa
    28621 aaggtggtat atgccatgag tgtgtcctgt tgggagttta atgccggata aggcatttta
    28681 cgccgcatcc tgtgcctgat gcgacgctgg cgcgtcttat caggcctaca aatccgagcc
    28741 gtaggccgga taaggcgttc acgctgcatc cggcacccgg agcctgatgc gacgctggcg
    28801 cgtcttatca ggcctacaaa ccgagccgta ggccggataa ggcgtttacg ccgcatccgg
    28861 catctcaata tcagatctta catgcgccgc tttcgcagcc atcgtcctgg attgacggta
    28921 ccagatcgtc ctgcgcgtct tcagcgccgt cacgggtgtt ctgataatac agcgttttga
    28981 ccccgaattt gtaggcggtg agcaggtctt tcagcaactg ctgcatcggc acttttcctg
    29041 acgggaaacg tgacggatcg tagttggtgt tagcagagat cgactgatcg ataaatttct
    29101 gcatgatacc taccagttgc aggtagccat cgttacccgg catttcccac agcagctcat
    29161 aggcgtcgtg caggtgctcg tagtccggca ccacctggcg caaaataccg tctttcgacg
    29221 ctttgatgct gacgtaaccg cgcggcggtt caataccgtt agtggcgtta gagatctgcg
    29281 aagaggtctc ggacggcatc agggcagaaa gcgtggagtt acgcaggccg tgcgttttga
    29341 ttgattcacg caatgcttcc cagtcgtaat gcagcggctc attagcgatg gcatccagat
    29401 ctttcttata ggtatcgatc ggcaggatcc ctttcgcgta agtggtttcg ttaaaccacg
    29461 ggcacgcgcc ttgctcttta gccagctcat tagacgcttt cagcaggtaa tactgaatcg
    29521 cttcgaaggt tttatgcgtc aggttgttgg cgctgccgtc ggagtagcgt ttaccgtgct
    29581 tcgccagata gtaagcgaag ttaatcacac caatacccag cgtacgacgc cccatcgctc
    29641 cacgtttggc ggccgggatc gggtaatcct gataatccag tagcgcgtca agtgcacgaa
    29701 ccgccagaat cgccagctct tccagttcat ccaggctatt aattgcgccc aggttgaaag
    29761 cagacagcgt acacagcgcg atttcaccgt tctcgtcgtt gacgtcgttc agcggtttgg
    29821 tcggcagggc aatttctagg cacaggttag actggcgaac tggcgcgatg gtcggatcaa
    29881 acggactatg ggtattacag tggtcaacgt tctgaatata gatacgaccg gtagacgcac
    29941 gttcctgcat catcagcgag aacagctcaa cggctttcac acgctgcttg cggatgctgt
    30001 cgtctttctc atatttggta tacaggcgtt caaactcttc ctgatcggcg aagaacgcgt
    30061 cgtacagccc cggtacgtcg gacgggctga acagggtgat atcttcacct ttcagcaggc
    30121 gggtatacat cagtttgttg atttgtaccc cgtagtccat atgacgcacg cggttgcctt
    30181 caaccccacg gttgtttttc aacaccagca ggctttccac ttccagatgc cacatcgggt
    30241 agaacagcgt tgccgcaccg ccgcgcacac cgccctgaga gcaggatttc accgctgtct
    30301 ggaaatgttt gtagaacggg atacagccgg tatggaacgc ttcgccaccg cgaatcgggc
    30361 tgcccagcgc acgaatacgt ccggcgttga tgccgatccc ggcacgctgg gaaacatatt
    30421 tcacaatcgc gctggaggtg gcgttgatgg aatccaggct gtcaccgcac tcgatcagta
    30481 cgcaggagct gaactgacga gtcggggtac gcacgccgga catgattggc gtcggcagcg
    30541 aaattttaaa tgtggaaacc gcgtcataaa aacgcttcac gtattgcagg cgcgtttcac
    30601 gcgggtagtt cgagaacagg cacgcggcaa caagaatata aaggaactgg gcgctttcgt
    30661 agatttcgcc ggtcacgcgg ttctgtacta gatatttgcc ttccagctgc ttaacggcag
    30721 cataagagaa ggtcatatca cggtcgtgat cgataaacgt gtccatctgc ttgaactctt
    30781 cttccgtgta gtcttccagc agatgattat cgtatttgcc catctcgacc attttcacca
    30841 cgtggtcgta cagcgcaggc ggctcaaact ggccgtaggc ttttttacgc aggtggaaga
    30901 tcgccaggcg cgcggcgaga tactgataat ccggcgcatc acgggagatc aggtctgcgg
    30961 cagccttgat aatagtttcg tggatgtcag aggtcttgat accgtcataa aactgaatgt
    31021 gggagcgcag ctcgacctgg gaaatcgaaa cgttatgcag tccttctgcc gcccaatcca
    31081 gaacgcgatg gattttgtcg agattgatgc gctctgtgct accgtcgcgc tttgtcacca
    31141 gcagattctg attcatgtat gtcgtacctg tttttggaat tctttccgca atagtttcat
    31201 gcagactacc tgtagtaagg gaagctgctt agaacactat atataggggg tatgtttggg
    31261 attcactgca agatagtgtg aaaatgaccc tcttgcaagt gcataacttt gtggataact
    31321 caggaaggaa aaagtggctt tcgcgcacct taggtcagac aaggtgtccg ggaaagtcaa
    31381 tgggaagaga aaaatttgtt aaaaataact gttcggcgat ttcttgaacg gcagaatagg
    31441 ttggataaat tgatatagat ggtttattaa tctttttgct aatgattgca aaaattgatc
    31501 taagcacgag tcatcacata atctgatgta tcagttgtgg tcaatatata agcgaatgta
    31561 agcgattgtt gatgtcgaaa tggttgtttt ctattcggtt attgttaaat gcgaagccat
    31621 tttgttaatt taccttatat tataagaaaa ttattcatta ctttaattta gtttaaatat
    31681 aactggtatc ccttaaggtt attaacttga attatgacgc actgatatta ttcatcaaat
    31741 aataacaaaa tagccattgc atcgggttgg acaatttaca
//
LOCUS       DAJTUP010000037        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTUP010000037 DAJTUP010000000
VERSION     DAJTUP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.037"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO468_001793"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO468_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR6258933.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO468_001794"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO468_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR6258934.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO468_001795"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO468_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR6258935.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO468_001796"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO468_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR6258936.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO468_001797"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO468_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR6258937.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO468_001798"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO468_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR6258938.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO468_001799"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO468_001799"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR6258939.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO468_001800"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO468_001800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR6258940.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO468_001801"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO468_001801"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR6258941.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO468_001802"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO468_001802"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR6258942.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO468_001803"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO468_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR6258943.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO468_001804"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO468_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR6258944.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO468_001805"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO468_001805"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR6258945.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO468_001806"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO468_001806"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR6258946.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO468_001807"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO468_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR6258947.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO468_001808"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO468_001808"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR6258948.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO468_001809"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO468_001809"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR6258949.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO468_001810"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO468_001810"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR6258950.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO468_001811"
     CDS             21622..22212
                     /locus_tag="OO468_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR6258951.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO468_001812"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO468_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR6258952.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO468_001813"
     CDS             22684..23379
                     /locus_tag="OO468_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR6258953.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO468_001814"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO468_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR6258954.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO468_001815"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO468_001815"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR6258955.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO468_001816"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO468_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR6258956.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO468_001817"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO468_001817"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR6258957.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO468_001818"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO468_001818"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR6258958.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7028 a         7823 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTUP010000038        29968 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTUP010000038 DAJTUP010000000
VERSION     DAJTUP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29968
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.038"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1582
                     /locus_tag="OO468_001819"
     CDS             <1..1582
                     /locus_tag="OO468_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR6258959.1"
                     /translation="LIGENAWGKSSLLDALTLLLSPESDLYHFERDDFWFPPGDINGR
                     EHHLHIILTFRESLPGRHRVRRYRPLEACWTPCTDGYHRIFYRLEGESAEDGSVMTLR
                     SFLDKDGHPIDVEDINDQARHLVRLMPVLRLRDARFMRRIRNGTVPNVPNVEVTARQL
                     DFLARELSSHPQNLSDGQIRQGLSAMVQLLEHYFSEQGAGQARYRLMRRRASNEQRSW
                     RYLDIINRMIDRPGGRSYRVILLGLFATLLQAKGTLRLDKDARPLLLIEDPETRLHPI
                     MLSVAWHLLNLLPLQRIATTNSGELLSLTPVEHVCRLVRESSRVAAWRLGPSGLSTED
                     SRRISFHIRFNRPSSLFARCWLLVEGETETWVINELARQCGHHFDAEGIKVIEFAQSG
                     LKPLVKFARRMGIEWHVLVDGDEAGKKYAATVRSLLNNDREAEREHLTALPALDMEHF
                     MYRQGFSDVFHRVAQIPENVPMNLRKIISKAIHRSSKPDLAIEVAMEAGRRGVDSVPT
                     LLKKMFSRVLWLARGRAD"
     gene            complement(1579..2535)
                     /locus_tag="OO468_001820"
     CDS             complement(1579..2535)
                     /locus_tag="OO468_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR6258960.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            2686..3801
                     /gene="macA"
                     /locus_tag="OO468_001821"
     CDS             2686..3801
                     /gene="macA"
                     /locus_tag="OO468_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR6258961.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            3798..5744
                     /gene="macB"
                     /locus_tag="OO468_001822"
     CDS             3798..5744
                     /gene="macB"
                     /locus_tag="OO468_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR6258962.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(5817..6041)
                     /gene="cspD"
                     /locus_tag="OO468_001823"
     CDS             complement(5817..6041)
                     /gene="cspD"
                     /locus_tag="OO468_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR6258963.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6364..6684
                     /gene="clpS"
                     /locus_tag="OO468_001824"
     CDS             6364..6684
                     /gene="clpS"
                     /locus_tag="OO468_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR6258964.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6715..8991
                     /gene="clpA"
                     /locus_tag="OO468_001825"
     CDS             6715..8991
                     /gene="clpA"
                     /locus_tag="OO468_001825"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR6258965.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9394..9481)
                     /locus_tag="OO468_001826"
     tRNA            complement(9394..9481)
                     /locus_tag="OO468_001826"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9445..9447),aa:Ser,seq:gga)
     gene            complement(9735..9953)
                     /gene="infA"
                     /locus_tag="OO468_001827"
     CDS             complement(9735..9953)
                     /gene="infA"
                     /locus_tag="OO468_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR6258966.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10238..10942)
                     /gene="aat"
                     /locus_tag="OO468_001828"
     CDS             complement(10238..10942)
                     /gene="aat"
                     /locus_tag="OO468_001828"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR6258967.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(10984..12705)
                     /gene="cydC"
                     /locus_tag="OO468_001829"
     CDS             complement(10984..12705)
                     /gene="cydC"
                     /locus_tag="OO468_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR6258968.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12706..14472)
                     /gene="cydD"
                     /locus_tag="OO468_001830"
     CDS             complement(12706..14472)
                     /gene="cydD"
                     /locus_tag="OO468_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR6258969.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14595..15560)
                     /gene="trxB"
                     /locus_tag="OO468_001831"
     CDS             complement(14595..15560)
                     /gene="trxB"
                     /locus_tag="OO468_001831"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR6258970.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16104..16598
                     /gene="lrp"
                     /locus_tag="OO468_001832"
     CDS             16104..16598
                     /gene="lrp"
                     /locus_tag="OO468_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR6258971.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16733..20761
                     /gene="ftsK"
                     /locus_tag="OO468_001833"
     CDS             16733..20761
                     /gene="ftsK"
                     /locus_tag="OO468_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR6258972.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            20916..21527
                     /gene="lolA"
                     /locus_tag="OO468_001834"
     CDS             20916..21527
                     /gene="lolA"
                     /locus_tag="OO468_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR6258973.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21538..22881
                     /gene="rarA"
                     /locus_tag="OO468_001835"
                     /gene_synonym="mgsA"
     CDS             21538..22881
                     /gene="rarA"
                     /locus_tag="OO468_001835"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR6258974.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            22972..24264
                     /gene="serS"
                     /locus_tag="OO468_001836"
     CDS             22972..24264
                     /gene="serS"
                     /locus_tag="OO468_001836"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR6258975.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24504..26947
                     /gene="dmsA"
                     /locus_tag="OO468_001837"
                     /pseudo
     CDS             24504..26947
                     /gene="dmsA"
                     /locus_tag="OO468_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            26958..27575
                     /gene="dmsB"
                     /locus_tag="OO468_001838"
     CDS             26958..27575
                     /gene="dmsB"
                     /locus_tag="OO468_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR6258976.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27577..28368
                     /locus_tag="OO468_001839"
     CDS             27577..28368
                     /locus_tag="OO468_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR6258977.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28404..29030)
                     /locus_tag="OO468_001840"
     CDS             complement(28404..29030)
                     /locus_tag="OO468_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6258978.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29345..>29968
                     /locus_tag="OO468_001841"
     CDS             29345..>29968
                     /locus_tag="OO468_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6258979.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNG"
BASE COUNT         7401 a         7402 c         8085 g         7080 t
ORIGIN      
        1 cctgattggg gagaacgcgt ggggtaaatc cagcttgctg gacgccttaa ccctgctgct
       61 atcgccagaa tcagatctct accattttga gcgcgacgat ttctggttcc cgccgggaga
      121 tatcaacggg cgagaacatc atctgcatat tattttgacc ttccgcgaat cgctgccagg
      181 ccgacatcgg gttcgccgtt atcggccgct ggaagcgtgc tggacgccat gcaccgatgg
      241 ctatcaccgt attttttatc gtctggaagg ggagagtgcg gaagacggca gcgtgatgac
      301 actgcgcagt tttctcgata aagacggaca tccgattgat gttgaggata ttaacgatca
      361 ggcacgccat ctggtgcgtt taatgccggt gctgcgcttg cgtgatgccc gttttatgcg
      421 ccgtattcgt aacggcacgg tgccaaatgt ccccaatgtg gaagtcaccg cgcgccagct
      481 cgatttcctc gcccgtgagt tgtcctcaca tccgcaaaat ctctctgatg gacagattcg
      541 tcagggactt tccgcaatgg tgcagttgct tgagcattat ttctctgagc agggggctgg
      601 acaggcgcga tatcgtttaa tgcggcggcg ggccagcaat gagcaacgaa gctggcgcta
      661 tctggacatc atcaaccgga tgattgaccg acctggaggg cgctcgtatc gggttatttt
      721 gctcggcctg tttgctactt tattgcaggc aaaaggcaca ttgcgactgg ataaagacgc
      781 gcgtccgtta ttgctaattg aagatccaga aacccgcctg catccgatta tgctttccgt
      841 tgcctggcat ctgttgaatc ttttgccttt acagcgtatt gctaccacca actctggcga
      901 gctgctttcg ttaactccag tggaacatgt ttgccgcctg gtgcgtgaat cttctcgtgt
      961 tgccgcctgg cgtctggggc caagtggctt gagcaccgaa gacagccgcc gcatctcttt
     1021 tcacattcgt tttaatcgtc cgtcatcgct gtttgctcgc tgctggttgc tggtggaagg
     1081 ggaaacggaa acctgggtta tcaatgaact ggcgcgtcag tgcggacatc attttgatgc
     1141 cgaagggatc aaggtcattg agtttgccca gtccgggcta aagccactgg ttaaatttgc
     1201 ccgccgaatg gggattgaat ggcatgtact ggtcgatggc gatgaagcag ggaagaaata
     1261 tgccgctacg gtacgcagcc tgttgaataa cgatcgggaa gccgaacgag aacatttaac
     1321 ggcgttaccg gcgctggata tggaacattt tatgtatcgc cagggatttt ccgatgtgtt
     1381 ccaccgcgtg gcgcaaatcc cggaaaatgt accgatgaat ctacgcaaaa ttatctcgaa
     1441 agcgatccat cgctcttcca aacccgatct tgccattgaa gtggcaatgg aggcaggacg
     1501 tcgtggtgtg gactccgtac caacgctgct gaaaaaaatg ttctcacgcg tgctgtggct
     1561 ggcgcgcggt cgcgcggatt aaccgcgaaa catcgtggcc atttgtggct gaatagcgtc
     1621 gagcatctca tagcgccgac ggtattcagc ccgtttttta ctggcgattt cggcaatctc
     1681 ttttcgtgct atctgtgctg gaaggcggta atggcgttca gcatcacata cgccgccaac
     1741 cgactcccag aacgcgttgt aatcagcatg gatcttgcct tctttatcgc gataacgcag
     1801 gctgcggtaa atatgcgttt cattgctgac ggcaataatc tgctctacct gcaaacgttg
     1861 ggcaaacaga caggccgctt ccatcacgag gcgtttggga aatagcccgt ggcaggcttt
     1921 cgtcgcattc tggatttcct gatgtggaat ttcccatttt gcgccttgca gtccgccaat
     1981 aaacatcgtt cttttcccct gatattcaca cagggtaaac gtgatctctg ccagaggaat
     2041 accttcgctg ttgcggaaca ggattgtgct gtcaccttct ttatccattg agatcatcat
     2101 ggtcagctca agcgtgaact gctcaccgtt tttgccttcc agtttagcca gttgcagccc
     2161 gggggtattc aaatataagc tgaattcttc cgccgacata cacccgcgga gtaacgcata
     2221 atggtaacgt aacgcctcca gcaattgctt acggctaaga ttcgcggcaa ggtaagggcg
     2281 atgcagacgc acaggcagtc gcggctggcg cgttaacaac acattgagat taggccagtg
     2341 ggaaagttcg ttcatccact caacgcttaa acgcggcata atcaacgagc gcagcaaaaa
     2401 tttctggcga aaactacagc gatgccagaa tttacccggc cgacactgtc cacgtgccag
     2461 actaagaaaa agtgacaggc tgctgagaga ttcagatggc gtaaaggtcc gttcagttag
     2521 ctgcgacata ttcatgaaat caatggttat acatgacgtc gatttcacca ttgcgtatct
     2581 taaccaaaca tcaatagtgt gattactaac gtaaatttta gggttttgtt gatatttcgt
     2641 tgaagttaat gacccggatt ggcatatgga gtattcagaa tatttatgaa aaagcggaaa
     2701 accgtgaaga agcgttacgt tattgcgctg gtgatagtca tcgccggact gattacgtta
     2761 tggagaattc ttaacgcacc cgtgccgact tatcagacac tgattgtgcg ccccggtgat
     2821 ttacagcaaa gcgtgctggc gaccggaaag ctggacgcgc tgcgtaaggt tgacgtgggc
     2881 gcgcaggtca gcggtcagtt gaaaacgctg tcggtggcga ttggcgataa agtaaaaaaa
     2941 gaccagcttt taggggtcat tgatcctgaa caggctgaaa accagatcaa ggaggtcgaa
     3001 gcaacgctga tggagctacg cgcgcagcgg cagcaggcgg aagcggagct gaaactggcg
     3061 cgggtgacgt attcccgtca gcaacgtctg gcacaaacgc aagcggtttc actgcaggat
     3121 ctcgataccg ccgcgacgga gatggctgtg aaacaggcgc aaattggcac cattgacgcg
     3181 caaatcaagc gcaatcaggc ctctctcgat acggctaaaa ccaatctcga ttacacccgt
     3241 atcgttgccc cgatggctgg ggaagtgacg caaatcacca ctctgcaagg ccagacggtg
     3301 attgccgcac agcaagcacc gaacattctg acgctggcag atatgagcac tatgctggta
     3361 aaagcgcagg tttctgaagc ggatgtaatc cacctgaagc cggggcaaaa agcctggttt
     3421 acggtgcttg gcgatccact gacgcgctac gaggggcaaa tcaaggatgt gctaccgacg
     3481 ccggaaaagg taaacgacgc tattttctat tacgcccgtt ttgaagtacc caaccccaat
     3541 ggtttgctgc ggctggatat gaccgcgcaa gtgcatattc agctcaccga tgtgaaaaat
     3601 gtgttgacga tccctctgtc ggcgttaggc gatccggttg gcgataatcg ttataaagtc
     3661 aaattgttgc gtaatggtga aacacgcgag cgtgaagtga cgattggcgc acgtaacgat
     3721 accgatgttg agattgtcaa agggctggaa gcgggcgatg aagtggtgat tggtgaggcc
     3781 aaaccaggag ctgcacaatg acgcctttgc tcgaattaaa ggatattcgt cgcagctatc
     3841 ctgccggtga tgagcaggtt gaggtgctga agggcatcag cctcaatatt tatgcgggtg
     3901 agatggtcgc gattgttggc gcttcgggtt ccggtaaatc gaccctgatg aatattctcg
     3961 gctgcctgga taaggccacc agcggcacct atcgcgtagc cggtcaggat attgccacgc
     4021 tggacgccga tgcgctggcg caactgcgcc gcgagcattt cggctttatt ttccagcgtt
     4081 accatttgct tccgcattta accgtcgagc agaacgttga agtacccgcc gtctatgccg
     4141 gtcttgagcg gaaacagcga ctgcttcgtg cccaggagtt gctgcaacgg ctggggctgg
     4201 aagaccgtac agagtattat ccggcacagc tttcgggtgg tcagcaacag cgcgtcagca
     4261 tcgcgcgggc attgatgaac ggcggtcagg taattcttgc cgatgaaccg accggcgcac
     4321 tggacagcca ttctggcgaa gaggtgatgg cgatcctgca tcagctgcgc gatcgcgggc
     4381 atacggtgat tatcgtcacc cacgatccgc aggtcgctgc tcaggccgag cgggtgatcg
     4441 aaattcgcga cggcgaaatt gtgcgcaatc ctcccgccat tgaaaaagtg aatgttgctg
     4501 gcgggacgga gcctgttgtc aatacagtgt ctggctggcg gcagtttgtc agcggtttta
     4561 acgaggcgct gacgatggca tggcgggcgc tggcagcgaa taaaatgcgt actttactga
     4621 ccatgctggg gattattatc ggtattgcgt cggtggtttc cattgtcgtg gtgggtgacg
     4681 ccgccaaaca aatggtgctg gcggatattc gttctattgg tacgaatact attgatgtct
     4741 atcccgggaa agattttggc gatgacgatc cgcaatatca gcaggcgctg aagtacgacg
     4801 acttaatcgc catccaaaaa caaccgtggg tcgcctcagc cacacctgcc gtctcgcaaa
     4861 acctgcgcct gcgttataac aatgttgatg ttgctgccag tgccaatggc gtgagcggcg
     4921 attattttaa tgtctatggc atgaccttca gtgaaggaaa cacctttaat caggagcagc
     4981 tgaacggtcg tgcgcaggtc gtggttctcg acagtaatac tcgccgccag cttttccccc
     5041 ataaagcaga tgtggttggc gaggtgattc tggtcggcaa tatgcccgcc agagtcattg
     5101 gtgtggcgga agaaaaacag tcgatgtttg gtagcagtaa agtgctgcgt gtctggctac
     5161 cttacagcac gatgtccggg cgagttatgg gccagtcgtg gcttaactcc attactgtca
     5221 gggtgaaaga aggatttgac agcgccgagg cggaacagca actcacgcgt ttactttcac
     5281 tgcgccacgg taagaaggat ttctttacct ggaacatgga cggcgtcttg aaaactgttg
     5341 aaaagaccac acgtacttta caactgtttc tgacgctggt ggcggtgatt tcgctggtgg
     5401 tgggcggtat tggtgtaatg aatattatgc tggtgtcagt gaccgagcgg acgcgggaaa
     5461 ttggcattcg catggctgta ggtgcgcgag caagcgatgt tttgcaacag ttcctgatcg
     5521 aagccgtact ggtttgcctg gtcggtggcg cgttgggaat aacactgtca ctgttaattg
     5581 ctttcacctt gcagcttttc ttacccggct gggagattgg tttttcaccg ttggcgctgc
     5641 tgctggcgtt tctctgctcg acggccaccg ggattttatt tggctggtta cccgcacgaa
     5701 atgcggcacg actggatcca gtagatgctc tggcacggga gtaatttttg agataaaaat
     5761 gccagccgat cgggctggca ttttgccttt aggatgtaca caatgagaca gaagagctat
     5821 gcgactgccg cttctacttc gacgggcaca ataacactgg cgtgattgcc ttttggcccc
     5881 tggtggacat caaactgaac ggattgtcca gcttttagcg ttctgtaacc atccatctga
     5941 atggtggaat aatgagcgaa aatatcttcg ccgccgcctt cagggcagat gaaaccaaac
     6001 cctttggcat tgttgaacca cttaacagta cccttttcca tgcttcgaca tccttcgcaa
     6061 atcttataca agtaagatgg aataaaccgg ggtcagagag ggggctgttc aaaacctcgc
     6121 caactctaga aatacaattt agagaattag ggcgagccgt caagcatttg acaggggcca
     6181 gggggcaggt atgaatcaaa aatttgaagc agttaacgct attgacagga atgtgacaga
     6241 tgtcgctgat gccaacgata gatgatagtt atctatcatg tggagtagat tggtcaggca
     6301 aataagctct tgtcagcggc agggcgttct gccgataacc gtaaccgaag atgataactg
     6361 acaatgggta aaacgaacga ctggctggac tttgatcaac tggcggaaga aaaagttcgc
     6421 gacgcgctaa aaccgccatc tatgtataaa gtgatattag tcaatgatga ttacactccg
     6481 atggagtttg ttattgacgt gttacaaaaa ttcttttctt atgatgtaga acgtgcaacg
     6541 caattgatgc tcgctgttca ctaccagggg aaggccattt gcggagtctt taccgccgag
     6601 gttgcagaaa ccaaagtggc gatggtgaac aagtacgcga gggagaatga gcatccattg
     6661 ctgtgtacgc tagaaaaagc ctgaatgcag gcataaaaat tgggggaggt gcctatgctc
     6721 aatcaagaac tggaactcag tttaaatatg gctttcgcca gagcgcgcga gcaccgtcat
     6781 gagtttatga ccgtcgagca cttgttactg gcgctgctca gtaacccatc tgcccgggag
     6841 gcgctggaag cgtgttctgt ggatttggtt gcgctccgtc aggaactgga agcctttatt
     6901 gaacaaacca cacccgttct gcctgccagt gaagaggagc gcgacacaca gccgacgctg
     6961 agttttcagc gtgtactgca acgtgcggtc ttccatgtcc agtcctccgg tcgcaatgag
     7021 gttaccggtg caaacgttct ggtcgctatc ttcagcgaac aggagtcgca ggcggcatat
     7081 ctgttgcgta aacatgaagt cagccgtctc gatgtggtga actttatctc tcatggcacg
     7141 cgtaaagacg agccgacaca gtcttctgat cctggcagcc agccaaacag cgaagaacaa
     7201 gctggtgggg aggaacgtat ggagaatttc acgacgaacc tgaaccagct tgcgcgtgtg
     7261 ggcggaatcg acccactaat tggtcgtgag aaagagctgg agcgtgctat tcaggttctc
     7321 tgccgtcgcc gtaaaaacaa cccgctgctg gtgggggaat ctggtgtcgg taaaaccgcg
     7381 attgcagaag gtcttgcctg gcgaattgtt cagggtgatg tgccggaagt gatggctgac
     7441 tgtacgattt actctctcga tatcggttct ctgttagcgg gcacaaaata tcgcggcgac
     7501 tttgaaaaac gttttaaagc gttgctcaag cagctggagc aggacactaa cagcatcctg
     7561 tttattgatg agatccacac cattatcggt gcgggtgcag cgtctggtgg tcaggtcgat
     7621 gcggctaacc taatcaaacc gttgctctcc agcggtaaaa ttcgcgtaat tggttcgaca
     7681 acctatcagg agttcagcaa cattttcgag aaagaccgtg ctctggcgcg tcgcttccag
     7741 aaaattgata ttactgaacc gtcgatcgaa gaaactgttc aaatcatcaa tggcctgaaa
     7801 ccgaagtatg aagcgcacca cgacgtgcgt tataccgcaa aagcggtgcg tgcggcggta
     7861 gagctggcgg tgaaatacat taacgatcgt catctgccgg ataaagccat tgatgttatc
     7921 gacgaagcag gcgctcgcgc acgtctgatg ccggtaagca aacgcaagaa aaccgttaat
     7981 gtggcggata ttgagtccgt ggtggcccgt attgcacgca ttccagagaa gagtgtttct
     8041 cagagtgacc gcgataccct gaaaaaactc agcgatcgcc tgaaaatgct ggtcttcggc
     8101 caggataaag ccattgaggc gctgactgaa gccattaaga tggcgcgtgc aggtttaggt
     8161 cacgaacata aaccggtcgg ttcgttcctg tttgccggtc ctaccggggt cgggaaaaca
     8221 gaggtgacgg tacagctttc gaaagcgttg ggcattgagc ttctgcgctt tgatatgtcc
     8281 gagtatatgg aacgccatac cgtcagccgt ctgattggtg cgcctccggg atacgttggt
     8341 tttgatcagg gcggtttgct gaccgatgcg gtaatcaagc atccacatgc ggtgctgctg
     8401 ctggacgaaa tcgagaaagc gcatccggac gtgttcaata ttctgttgca ggtgatggac
     8461 aacggtacgc tgaccgataa caacggacgc aaagcggact tccgtaacgt ggtgctggtg
     8521 atgaccacta acgccggggt acgggaaact gagcgcaaat ccattggtct tatccaccag
     8581 gataacagca ccgatgcgat ggaagagatc aagaagatct ttacaccgga attccgtaac
     8641 cgtctcgaca acattatctg gttcgatcat ctgtcaactg acgtgatcca tcaggtggtg
     8701 gataaattca tcgtcgagtt gcaggttcag ctggatcaga aaggtgtttc tctggaagtg
     8761 agccaggaag cgcgtaactg gctggccgag aaaggttacg accgggcaat gggcgcacgt
     8821 ccgatggcgc gtgtcatcca ggacaacctg aaaaaaccgc tcgccaacga actgctgttt
     8881 ggttcgctgg tggacggcgg tcaggtgacg gttgcgctgg ataaagagaa aaatgagctg
     8941 acttacggat tccagagtgc acaaaagcac aaggcggaag cagcgcatta atctgattgt
     9001 caggtaggtt ggtgaagtcc gtaatctcgt caggggttac ggacttttta tttatggggt
     9061 ggaacggtga aaaccctatt tttggaggtg aaggtaagtt gttgataatt agtgctgctg
     9121 gaaggtaagg ataaaaaagg gtgctgcagg agaatgggat ggttttgctt tattaacaac
     9181 gggctaaacg tgtagtattt gagttcactg ccgtacaggc agcttagaaa tcgacggggt
     9241 gcggtaaaac ctttgcgaac gcgttcactg ccgtacaggc agcttagaaa ttcacaggta
     9301 acatactcca cccgcccacc atgttcactg ccgtacagac agataaaatg cgaaaaaaaa
     9361 gctcgcactt tcgtacgagc tcttctttaa atatggcggt gaggggggga ttcgaacccc
     9421 cgatacgttg ccgtatacac actttccagg cgtgctcctt cagccactcg gacacctcac
     9481 caaattgttt tgctaccaaa cctcatgggt ggcaacgggg cgctactata gggagttgga
     9541 gtaaaacggt caagaagaat tttaatgata attattgttt gctcatactg taaacaactt
     9601 gtgcagtata tctacatcga gacaagttat ggacttatac ttccaaagta cttcatacat
     9661 atcacaaaat aaaaaggccg gttaaaccga ccttttactc gttctttctc ttcgcccatc
     9721 aggcggtaaa acaatcagcg actacggaag acaatgcggc ctttgctcag gtcgtacggg
     9781 gtcagttcaa cagtcacttt gtcgcccgtc aggatgcgga tgtagttttt gcgcatttta
     9841 ccggagatgt gtgcagtaac cacgtgaccg ttttctaact ctacgcggaa catggtatta
     9901 ggcaacgttt caagaacggt accttgcatt tcaatattgt cttctttggc catctaatcc
     9961 tctggggtat cactaccgta atttgaaccg gcaagataat gccgaagttc tgtaaataag
    10021 taaagatttg cgcgctaaat cgcaacaaac aggttcggca cattactccg aaaacacacg
    10081 gctaagccgc accaaaagcg caacgtataa gggagcggtg agataaacga tgggcgttac
    10141 ctgacgcgaa aaattcctta tcggcagcgg ggtaatgagc gtaaccaact ctgcgaccgc
    10201 aattataaca ctctggggag aaatgtgccg aaaacattca ttcttgtggt gaaaacaagc
    10261 accgtggtac ccagaaatta ttcggcaatc gtccgaggcg catttgattg agataattaa
    10321 ggtaatcccg gcggggaatt tcgcaggcac caagcgatgc tgtgtgatcg ttaaggacct
    10381 ggcagtcgat aagcttaccg ccatgaccga taaattcctc acagaatacc agaagcgccg
    10441 ttttagacgc attttccatc cggctgaaca tggactcgcc acaaaatagc gttccctggg
    10501 ccacgccgta cataccgccg acaagctcat cttcacgcca gacttcaatg gagtgggcat
    10561 gaccgagttc gtgaaggcga tggtaggctt cgaccacgcc acgcgtgatc caggttcctt
    10621 cttcgcgatc gctggcacag ccttcaatga cctgaccaaa agcgtaattc atcgtgacac
    10681 gatagggcga gcgtttatga aatcgcttca tactacggct gatatgcagt gattctggcc
    10741 atagcaccgc gcggggatcg ggcgaccacc agaggatggg gtcgcctgga gaaaaccacg
    10801 gaaaaatacc acgctggtaa gccattaaca ggcgcgcagg gctaagatcg cccccaagtg
    10861 ccagcaggcc gttaggctca cgtaatgcgc cttccgggga agggaaggct attgaatggc
    10921 gagaaagctg aaccaagcgc atgaccgcaa aactccacgc aagtcggatc gttcaataat
    10981 agcttacaaa ccctgcttga actggtaata acgcccctgt ctggcaagca gttctgcgtg
    11041 agtaccttgc tcaataattt gcccgttgtc catcactatt atttgttgga aacgagagag
    11101 tccgcgaagt cgatgggtga ccattaacac cgttttctca cgcatcattt ttgcaagcaa
    11161 ttcaaggatc tggctttcgg ttgtggcatc taagccttcg gtaggttcat ccagcaacac
    11221 cagtggcgca tcatgtaaca gcgtacgggc gatagccaga cggcgcagtt caccaccgga
    11281 gagctggcgt ccgccttcac ctaaccaact gttgagacct gcatcctcga gcagcttctc
    11341 cagaccaacg cgacgcaaga tctccgacag agcctcatca ctactgccag gcgaggcgag
    11401 taacagatta tcacgcagtg tggcgctaaa cagatgcact cgctgaggaa caacgctgat
    11461 ggtctgtcgt agagccgctt cattcaggct ggctatgggg ctatcgttaa gcaaaatctc
    11521 gccctgttgc gggtcccatg cgcgggtcag cagttgtaac agtgttgatt tgccgcatcc
    11581 ggttcgcccg agaatcgcta tatgttcccc ggcgtttacc tgaagagaaa tccctttaag
    11641 tgcctgttgg gattgctccg gataagtgaa ctgaacatcc cgtaatgtca gcgaaacgcg
    11701 atcggcaaca cgagtttggg tatcagggaa ggtgacctcc ggtttttgat ccgttaagtc
    11761 agagatacgt acggcagagg caatgacttg ccccagatgc tgaaatgcac ccgttactgg
    11821 tgccagtgct tcaaacgcgg ctaacgcaca aaagacaaac agggcaatta atgcgccggg
    11881 ttgagcattg ccgccaacgc cgccagacgc catccacagc atcaggatca ccgctaacgc
    11941 gccaatgagc agcattatcg cttgcgacaa tgcggtcagt tcagattgac ggcgttgcgc
    12001 ttccagccat tgaatttctg tattctctag ttgcgtgcga taacgatcgc tggcaccaaa
    12061 aatggtcagc tcagcttgcc cttgcagcca ggccgtcagt tgttggcgat actgtccgcg
    12121 aagatgagtc agattttgcc cggtgctttt tcccgcacga taaaacagcg gtggcatcag
    12181 gaaaagcgtc agtaacataa cgctgcccag cgtaaaggcg agggtgaaat caaggaaact
    12241 taacccgatt gtcaccacca taatcaccac aaaagcgccc accagcggcg agataacgcg
    12301 caggtaaaga tgatcgagcg tatcaacatc cgccaccacg cggttgagca attcgccctg
    12361 acgatagcgc gccagtccgg caggggagag gggcaacaat ttgctgaagg tgtaaatgcg
    12421 cagatgctgc aacacgcgga aagtcgcgtc gtgacttacc agacgttcaa aataacgccc
    12481 ggcagtacgg gtgattgcgg caccacgcac gcccgcagcg ggtagcatat agttgaagct
    12541 gtacagtccg gcaacccccg caaccgctga ggccgagagg aaccagccgg aaagtgtcaa
    12601 cagaccgata ctggcgagca gcgtcacaat tgccagcaca ataccaagac ttaacatcca
    12661 tttatgacgt ttatacagtg ccagataggg tagcaaagcg cgcatttaaa tctcctcctg
    12721 acgatgggcc agtaatgtgg cgaatgggcc accagccaca cttaattccg cgtaacgtcc
    12781 ttgctcaata atccggccat cctgcataac ccaaatgaca tcccagtcag caagatcttc
    12841 taactggtgg gtgaccatta acgttgtctg gcgcagagag gcggcattca gcgcctccat
    12901 tacgcgctgt tcactgtgag catcaaggct ggcagcgggt tcatccaaca gtaatagcga
    12961 acagggattt agtaacgcac gggccaccgc cacgcgctgc gcctgcccca cggaaaggcg
    13021 ggcagcctgg tcgccaacag gtgtatcaac gccttgtggc aggagcggta gaaactcgct
    13081 gacccaggcg ttatccagcg ctgcttgtaa ttcttgttcg ctggcatcag gtcgcgccag
    13141 tagtacgtta tcccgcaatt ttgctgccgg taattgtggg ttttgcccaa cccaggagag
    13201 atgtttacgc catgattctg gtgataaatc acgtaattct atcccgttga ttcgtaatga
    13261 tccctgatat gagagaaaac cagaaagcgc gttcagcagt gagcttttac ctgaaccgct
    13321 gcggccaacc aacactgcac gttggcctgc tggcaaagta aagttcagcg gtccggccag
    13381 cgttttacct tccggcgacg tgataaacag atcctcggcc tcaatggtca gcggatcggt
    13441 caatgctaat tccgcctcac cgcgctgcgg atgggcgagc ggggtttcca taaacgtttt
    13501 cagactgtca gctgcaccaa cagcctgggc tttagcatga taaaacgtac cgagatcgcg
    13561 taatggctgg aaaaactctg gcgcaaggat cagggccaga aaacctgcag ccagcgtcac
    13621 cccagtatcg tagtgaccaa aatccagctc gccgagatag gaaaaaccaa agtagaccgc
    13681 caccagagca attgacagcg aggtaaaaaa ttcgagaatg ccggaggata aaaacgccag
    13741 tcgtagcact tccattgtcc gttggcggaa atcttccgaa gcagaacgaa tactttcaat
    13801 ttcagcttca ccacgaccaa aaatacgcaa tgtttccatg ccgcgcaggc gatcgaggaa
    13861 atgcccactt aagcgagcaa gagcgagaaa gttacgccgg ttagcatcgg cagcccccat
    13921 tccaaccagc gccataaaca acggaattag cggtgctgtg cccagcagaa tgagcgccgc
    13981 agcccagtta gaggggaaga tcgccaccac aatcagcaac ggcaccgaca ctgccagcgc
    14041 catttgcggc agatagcgtg catagtaatc atgcatatcg tcaatttgct cgagtaccag
    14101 cgtcgcccag ctccccgcag gtttaccctg aatccacgct ggccctgctt gttgcagacg
    14161 gtcgagaacc tgacggcgga tggcaaagcg gatatgctgc ccggcgtgat aacccacccg
    14221 ttcgcgtaac cagaccaccc atgcgcgcaa tacaaaggtc agaaccagta acgtaaaggg
    14281 aagcagcagg gcttcacggg gaatattctc cataatcata tgttgcagaa ttcgcgccat
    14341 gaaccaggcc tgggcaatga tcaatatgcc gcttacaaag cccagcagac gagaaatatt
    14401 cagccaacgt tgggagatga cgctttgctg ttttaaccag cgggttaact ctttttgacg
    14461 agatttattc attgcacgct tagcaggtga gttatcagaa ttatttgcag agcaatgtta
    14521 caacggggaa aaaataaagg cgacccatag tcgcaaggtg tcgccttctt tacttttgtt
    14581 actgatttgt aaaattattt tgcgtcagct aaaccatcga ggtagcgttc cgcatcaagt
    14641 gctgccatgc agccagtacc ggccgaagta atggcctggc gataaatgtg atccatcacg
    14701 tcgcctgcgg caaagacgcc aggaatgctg gtctgggtgg cattaccatg aatacccgac
    14761 tgtactttga tgtagccgtt ttccagttcc agctgccctt cgaaaatcgc agtattcggg
    14821 ctgtgaccga tagcaacaaa cagaccggca acgtcgagtg actcgatgtt atcgctgttt
    14881 tgcgtatcgc gcagacgaac gccagtgaca cccatttgat cgccggtcac ttcttccagc
    14941 gtacggttgg tgtgcagaat gatgttgccg ttctccactt tatccatcag acgcttaata
    15001 aggatttttt ccgcgcggaa accgtcacgg cggtgaatca gatgcacttc cgaagcgatg
    15061 ttagacagat acagcgcctc ttcaaccgcg gtattgccgc cgccgatgac cgcaactttc
    15121 tggttgcgat agaagaaacc gtcgcaggtt gcacaagcag aaaccccacg gcctttaaag
    15181 gcttcttcag agggcaggcc gagatagcgt gcagaagctc cggtggcaat aatcagcgcg
    15241 tcgcaagtgt attcgccgtt atcgccattc agacggaacg gacggttttg cagatccacc
    15301 ttgttgatat gatcaaaaat gatctcagtt tcaaacttgg tggcatgttc gtgcatgcgc
    15361 gccattaata acggaccggt cagatcgttt ggatcaccag gccagttttc cacttccgtg
    15421 gtggtggtca gttggccgcc tttttccatg ccggtaatca gcacaggttg caggttggcg
    15481 cgcgccgcgt agacagcagc ggtgtatccc gccgggcctg aacccaggat aagcagttta
    15541 ctgtgtttgg tcgtgcccat gagatcccca tagttgttgg cagacaatgg gcaggattgt
    15601 agggaattta cagacgtaaa aaaagagtat gacgattttg ttaacaattt gtgcaatcgg
    15661 cagcatcgat aagcaggtca aattctcccg tcattatcac ctctgctact taaatttccc
    15721 gctttataag ccgattaaat gatgaataaa cgcccctgtt aatgaatatc tggcatgttg
    15781 tactaaaaat cgatgttttg ctttgacaat cccctggtgt tttgcgaaaa cattcgagga
    15841 agaaaaaaac agtattctta tatgcgcata accatgtatg taaataccat gtttaccgtg
    15901 ctagtgaaat ctacgtatgg cgtggacaga cgccattcgt gatgtcgata gctgccacaa
    15961 ggcaacggtc ttctcaccgt agacccaggc attgcgcgcc gtgaatcttc atgatttcgg
    16021 tctatcgtga cgggtagcga ctctgaacag tgatgtttca gggtcagaca ggagtaggga
    16081 aggaatacag agagacaata ataatggtag atagcaagaa gcgccctggc aaagatctcg
    16141 accgtatcga tcgtaacatt cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg
    16201 agctttctaa acgtgtggga ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg
    16261 aaagacaagg gtttattcag ggctatacgg cgctgcttaa cccccattat ctggatgcat
    16321 cacttctggt attcgttgag attactctga atcgtggcgc accggatgtg tttgaacaat
    16381 tcaataccgc tgtacaaaaa cttgaagaaa ttcaggagtg tcatttagta tctggtgatt
    16441 tcgactacct gttgaaaaca cgcgtgccgg atatgtcagc ttaccgtaag ttgctggggg
    16501 aaaccctgct gcgtctgcct ggcgtcaatg acacacggac atacgttgtt atggaagaag
    16561 tcaagcagag taatcgtctg gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg
    16621 cgtattttga ttacactcct gttaatccat acagcaacag tactggggta acctggtact
    16681 gttgtccgtt ttagcatcgg gcaggaaaag cctgtaacct ggagagcctt tcttgagcca
    16741 ggaatacact gaagacaaag aagtcacatt gacaaagtta agtagcggcc gccgccttct
    16801 ggaggcattg ctgatcctta ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag
    16861 ctttaaccct tcggacccca gctggtcgca aacggcctgg catgaaccta tccataattt
    16921 aggtgggatg cccggtgcgt ggttggcaga tacgctgttc tttatttttg gcgtgatggc
    16981 ttacaccatt cccgtcatta ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag
    17041 cgacgaatac attgattatt ttgccgtttc gctacgcatc attggcgttt tggcgctcat
    17101 ccttacctcc tgtggtctgg cggcaatcaa cgctgacgat atctggtatt ttgcctccgg
    17161 tggcgtcatt ggcagcttac taagcactac gctacaacca ctgctacaca gtagcggggg
    17221 aactattgcg ctgctctgcg tttgggcagc gggcctgacg ttgttcaccg gttggtcatg
    17281 ggtgaccatt gctgaaaaac tcggcggctg gattttaaac attctcacct tcgccagtaa
    17341 tcgtacccgt cgcgatgata cctgggtcga tgaagatgag tatgaagacg acgaagagta
    17401 tgaagatgaa aatcacggca aacagcatga atcacgccgt gcccgtattc ttcgcggcgc
    17461 gctagcgcgt cgtaaacggt tggcggaaaa attcattaat ccgatggggc ggcaaacaga
    17521 cgctgcgttg ttctccggta agcggatgga tgatgacgaa gagattacct acactgcacg
    17581 cggtgtggct gccgacccgg acgacgtcct attttcgggc aatcgtgcaa cgcagccaga
    17641 atatgacgaa tacgatccat tattaaacag tgcgccaatt accgaacctg tcgctgtagc
    17701 agctgctgct accacggcga cacaaagctg ggctgcgccg gttgaacctg tgactcagac
    17761 gccgcctgtt gcctctgttg atgttccacc ttcgcaacct acagtagcct ggcagcctgt
    17821 accgggtcca caaacgggag agccggttat tgctcctgca ccggaaggtt acccacagca
    17881 gtcacaatat gcgcagcctg cagtgcaata taatgagccg ctgcaacaac cagtacagcc
    17941 gcagcagccg tattatgcac ctgcagctga acaacctgcg caacagccgt attatgcccc
    18001 tgcgccagaa caaccggtgg caggtaacgc ctggcaagcc gaagagcagc aatccacttt
    18061 tgctccacag tctacatacc agactgagca aacttatcag cagccagccg ctcaggagcc
    18121 gttgtaccaa cagccgcaac ccgttgaaca gcagcctgtt gtggagcctg aacccgttgt
    18181 agaagagaca aaacccgcgc gtccgccgct ttactatttt gaagaagtgg aagagaagcg
    18241 agcccgtgaa cgtgaacaac ttgcggcctg gtatcaaccg attccagaac cggttaaaga
    18301 accagaaccg atcaaatctt cgctgaaagc accttctgtt gcagcagtac ctccagtaga
    18361 agccgctgcc gctgtttctc cgctggcatc tggcgtgaaa aaagcgacac tggcgacggg
    18421 ggctgccgca accgttgccg cgccagtctt cagtctggca aatagcggtg gaccgcgtcc
    18481 gcaggtcaaa gaggggattg gcccgcagtt gccacgaccg aaacgtatcc gcgtgccaac
    18541 tcgtcgtgaa ttggcgtctt acggtattaa gctgccctca cagcgtgcgg cggaagaaaa
    18601 agcccgtgaa gcccagcgca atcagtatga ttctggcgat cagtataacg atgatgaaat
    18661 cgatgcgatg cagcaggatg aactggcacg tcagttcgcc cagacacagc agcaacgcta
    18721 tggcgaacag tatcaacatg atgtgcccgt aaacgcagaa gatgcagatg ctgcggcaga
    18781 ggctgaactg gctcgtcagt ttgcgcaaac tcaacaacaa cgttattccg gcgaacaacc
    18841 ggctggggcg aatccgttct cgctggatga ttttgaattt tcgccaatga aagcgttgct
    18901 ggatgatggt ccacacgaac cgctgtttac gccaattgtt gaacctgtac agcagccgca
    18961 acaaccggtt gcaccacagc agcagtatca gcagccacaa cagccggttg cgccacagca
    19021 gcagtatcag cagccacaac agcaggttgc gccgcagcca caatatcagc agccgcaaca
    19081 accggttgcg ccacagcagc aatatcagca gccgcaacaa ccagttgcgc cgcagccgca
    19141 gtatcaacag ccgcaacaac cggttgcacc gcagccgcag tatcagcagc cacaacagcc
    19201 agttgcgcca cagccgcagg ataccctgct tcatccgctg ttgatgcgta atggcgacag
    19261 ccgtccgttg cacaaaccga cgacgccact gccttctctg gatttgctga caccgccgcc
    19321 gagcgaagtg gagccggtag atacctttgc gcttgaacaa atggcgcgtc tggtggaagc
    19381 gcgtctggct gatttccgta ttaaagccga tgtcgttaat tactctccgg ggccagttat
    19441 cactcgcttt gaacttaacc tggctcctgg cgtaaaagcg gcgcgcattt ctaacttgtc
    19501 acgggacctt gcccgttcac tttcgacggt ggcggtgcgt gtcgttgaag ttattcctgg
    19561 caaaccctat gtaggtctgg agttaccaaa taaaaaacga caaaccgttt atctgcgcga
    19621 agttttggat aacgccaaat tccgcgataa tccgtcgcca ttaaccgtgg tgctgggtaa
    19681 agatatcgcc ggtgagccgg tggttgccga tctggcgaaa atgccgcact tgttggttgc
    19741 ggggactacc ggttccggta aatctgtcgg tgtgaacgcg atgatcctga gcatgcttta
    19801 taaagcacag ccagaagatg tgcgtttcat catgatcgac ccgaaaatgc tggagctttc
    19861 ggtttatgaa ggcattccgc acctgttaac ggaagtcgtt actgatatga aagatgccgc
    19921 caacgcgctg cgctggtgtg ttaacgagat ggagcgtcgg tataaactga tgtctgcgct
    19981 gggtgtgcgt aatctggcgg gttataacga aaaaattgct gaagccgatc gcatgatgcg
    20041 tccgattcca gacccgtact ggaagccggg tgacagtatg gatgcccagc atccggtgct
    20101 gaaaaaagaa ccatacattg tcgtgttggt tgacgaattt gccgacctga tgatgacggt
    20161 aggtaaaaaa gtggaagagc tgatagctcg tctggcgcaa aaagcccgtg ccgcgggtat
    20221 ccacctcgta ctggcaactc agcgtccatc ggttgatgtt attactggtc tgattaaagc
    20281 gaatattccg acccgtatcg cctttaccgt atccagtaag attgactcac gtaccattct
    20341 tgatcaggct ggcgcggaat cactgctggg tatgggggat atgctctact ctgggccgaa
    20401 ctccacgttg ccggtacgtg tccatggtgc ttttgttcgc gatcaggaag ttcatgccgt
    20461 ggtgcaggac tggaaagcgc gtggtcgccc acagtatgtt gatggcatca cctccgacag
    20521 cgaaagcgaa ggtggtgcgg gtggtttcga tggcgctgaa gaactggatc cgttgttcga
    20581 tcaggcggtg cagtttgtca ctgaaaaacg caaagcgtca atttctggcg tacagcgtca
    20641 gttccgcatt ggttataacc gtgcagcgcg tattatcgaa cagatggaag cgcaggggat
    20701 tgtcagcgaa caggggcaca acggtaatcg tgaagtgctg gccccaccgc cgtttgacta
    20761 attaatgcat tgccggataa ggcgcggtag cgtcgcatcc ggcactctat caactgaaaa
    20821 ttcagtattt tcttctttcc tcaagctgat tattagcctg gaatagagag tagagggaac
    20881 tcccgatcgg gagtgacgta atttgaggaa taatgatgaa aaaaattgct atcacctgtg
    20941 cattactctc aagcttagta gcaagcagcg tttgggctga tgccgcaagc gatctgaaaa
    21001 gccgcctgga taaagtcagc agcttccacg ccagcttcac acaaaaagtg actgacggta
    21061 gcggcgcggc ggtgcaggaa ggtcagggcg atctgtgggt gaaacgtcca aacttattca
    21121 actggcatat gacacaacct gatgaaagca ttctggtttc tgacggtaaa acactgtggt
    21181 tctataaccc gttcgttgag caagctacgg caacctggct gaaagatgcc accggtaata
    21241 cgccgtttat gctgattgcc cgcaaccagt ccagcgactg gcagcagtac aatatcaaac
    21301 agaatggcga tgactttgtc ctgacgccga aagccagcaa tggcaatctg aagcagttca
    21361 ccattaacgt gggacgtgat ggcacaatcc atcagtttag cgcggtggag caggacgatc
    21421 agcgcagcag ttatcagctg aaatcccagc aaaatggggc tgtggatgca gcgaaattta
    21481 ccttcacccc gccgcaaggc gtcacggtag atgatcaacg taagtagagg cacctgagtg
    21541 agcaatctgt cgctcgattt ttcggataat acttttcaac ctctggccgc gcgtatgcgg
    21601 ccagaaaatt tagcacagta tatcggtcag caacatttgc tggctgcggg gaagccgttg
    21661 ccgcgcgcta tcgaagccgg gcatttgcat tctatgatcc tctgggggcc accgggtacc
    21721 ggcaaaacaa ccctcgctga agtgattgcc cgctatgcga acgctgatgt ggaacgtatt
    21781 tctgccgtca cctctggcgt gaaagagatt cgcgaggcga tcgagcgcgc ccggcaaaac
    21841 cgcaatgcag gtcgccgcac tattcttttt gttgacgaag ttcaccgttt caacaaaagc
    21901 cagcaggatg catttctgcc acatattgaa gacggcacca tcacttttat tggcgcaacc
    21961 actgaaaacc cgtcgtttga gcttaattcg gcactgcttt cccgtgcccg tgtttatctg
    22021 ttgaaatccc tgagtacaga ggatattgag caagtactaa ctcaggcgat ggaagacaaa
    22081 acccgtggct atggtggtca ggatattgtt ctgccagatg atacacgacg tgccattgca
    22141 gaactggtga atggcgacgc gcgccgggcg ttaaatacgc tggaaatgat ggcggatatg
    22201 gccgaagtct atgatagcgg taagcgggtc ctgaagcctg aattactgac cgaaatcgcc
    22261 ggtgaacgta gcgcccgctt tgataacaaa ggcgatcgct tttacgatct aatttccgca
    22321 ctgcataagt cggtacgtgg tagcgcaccc gatgcggcgc tgtactggta tgcgcgaatt
    22381 attaccgctg gtggcgatcc gttatatgtc gcgcgtcgct gtctggcgat tgcgtctgaa
    22441 gacgtcggta atgccgatcc acgggcgatg caggtggcaa ttgcggcctg ggattgcttt
    22501 actcgcgttg gcccggcgga aggtgaacgc gccattgctc aggcgattgt ttacctggcc
    22561 tgcgcgccaa aaagcaacgc tgtctacact gcgtttaaag ccgcgctggc cgatgctcgc
    22621 gaacgcccgg attatgacgt gccggttcat ttgcgtaatg cgccgacgaa attaatgaag
    22681 gaaatgggct acgggcagga atatcgttat gctcatgatg aagcaaacgc ttatgctgcc
    22741 ggtgaggttt acttcccgcc ggaaatagca caaacacgct attatttccc gacaaacagg
    22801 ggccttgaag gcaagattgg tgaaaagctc gcctggctgg ctgaacagga tcaaaatagc
    22861 cccataaaac gctaccgtta atgttatcgt tgcggtaatg ttgttactgt atccctgtgg
    22921 tcgcaggctg tggccacatc tcccatttaa ttcgataagc acaggataag catgctcgat
    22981 cccaatctgc tgcgtaatga gccagacgca gtcgctgaaa aactggcacg ccggggcttt
    23041 aagctggatg tagataagct gggcgctctt gaagagcgtc gtaaagtatt gcaggtcaaa
    23101 acggaaaacc tgcaagcgga gcgtaactcc cgatcgaaat ccattggcca ggcgaaagcg
    23161 cgcggggaag atatcgagcc tttacgtctg gaagtgaaca aactgggcga agagctggat
    23221 gcagcaaaag ccgagctgga tgctttacag gctgaaattc gcgatatcgc gctgaccatc
    23281 cctaacctgc ctgcagatga agtgccggta ggtaaagacg aaaatgacaa cgttgaagtc
    23341 agccgctggg gtaccccgcg tgagtttgac tttgaagttc gtgaccacgt gacgctgggt
    23401 gaaatgcact ctggcctcga ctttgcagct gcagttaagc tgactggttc ccgctttgtg
    23461 gtaatgaaag ggcagattgc tcgcatgcac cgcgcactgt cgcagtttat gctggatctg
    23521 cataccgaac agcatggcta cagtgagaac tatgttccgt acctggttaa ccaggacacg
    23581 ctgtacggta cgggtcagct gccgaaattt gctggcgatc tgttccatac tcgtccgctg
    23641 gaagaagaag cagacaccag taactatgcg ctgatcccaa cggcagaagt tccgctgact
    23701 aacctggtgc gcggtgaaat catcgatgaa gatgatctgc caattaagat gaccgcccac
    23761 accccatgct tccgttctga agccggttca tatggtcgtg acacccgtgg tctgatccgt
    23821 atgcaccagt tcgacaaagt tgaaatggtg cagatcgtgc gcccagaaga ctcaatggcg
    23881 gcgctggaag agatgactgg tcatgcagaa aaagtcctgc agttgctggg cctgccgtac
    23941 cgtaaaatca tcctttgcac tggcgacatg ggctttggcg cttgcaaaac ttacgacctg
    24001 gaagtatgga tcccggcaca gaacacctac cgtgagatct cttcctgctc caacgtttgg
    24061 gatttccagg cacgtcgtat gcaggcacgt tgccgcagca agtcggacaa gaaaacccgt
    24121 ctggttcata ccctgaacgg ttctggtctg gctgttggtc gtacgctggt tgcagtaatg
    24181 gaaaactatc agcaggctga tggtcgtatt gaagtaccag aagttctgcg tccgtatatg
    24241 aacggactgg aatatattgg ctaataccca atttttctga atctaaaaag cgcctttagg
    24301 gcgctttttt tgtctccctt tgataccgaa caataattac tcctcactta cacgtaatac
    24361 tactttcgag tgaaaatcta cctatctctt tgattttcaa attattctat gtatacaagc
    24421 ctatatagcg agctgctata gaaataatta cacaatacgg tttgttactg gaatcaatcg
    24481 tgatcaagct tgagtgagcc attatgaaaa cgaaaatccc tgatgcggta ttggctgctg
    24541 aggagagtcg ccgtggtttg gtaaaaacga cagcgatcgg cggcctggca atggccagca
    24601 gcgcattaac attacctttt agtcggattg cgcacgctgt cgatagcgcc attccaacaa
    24661 aatcagacga aaaggttatc tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc
    24721 tacgtatgca cgtcgtggac ggtgaaatca aatatgtcga aacgacaata ccggcgatga
    24781 caattacgac ggcctacact aggttcgcgc ctgcctgcgt gggcgttcca tgcgtcaccg
    24841 tgtctacaat ccggaccgcc tgaaatatcc gatgaaacga gtcggggcgc gcggtgaagg
    24901 caaattcgag cgcattagct gggaagaagc ctacgacatc atcgcgacca atatgcagcg
    24961 cctgatcaaa gagtacggca acgagtctat ctatctgaac tatggcaccg gtacgctggg
    25021 cggcaccatg acccgctcct ggccgccggg aaataccctg gtcgcgcgcc tgatgaactg
    25081 ctgcggcggt tatcttaacc attacggtga ctactcttcc gcgcaaattg cggaaggttt
    25141 gaaccatacc tacggcggct gggcagatgg taacagcccg tcggatatcg aaaacagtaa
    25201 attggtagtg ctgtttggta ataacccagg cgaaacgcga atgagtggcg gtggggtgac
    25261 ttactatctt gaacaggcac gccagaaatc taatgctcgc atgatcatca tcgatccgcg
    25321 ctataccgac accggtgccg ggcgcgaaga tgagtggatc cctattcgtc caggaacaga
    25381 tgccgcactg gttaacggtc tggcgtacgt catgatcact aaaaacctgg tggatcaggc
    25441 attcctcgat aaatattgcg ttggctatga cgagaaaacc ctgcccgcca gtgcgccgaa
    25501 aaatggtcat tataaagctt atattctggg tgaagggcca gatggcgtgg ctaaaacacc
    25561 ggaatgggcc tcgcaaatca ccggtgttcc ggcagacaaa atcatcaaac tggctcgtga
    25621 aatcggcagt accaaaccgg cgtttatcag ccagggatgg ggcccgcagc gtcacgctaa
    25681 cggtgaaatc gcaacccgtg ctatctcgat gctggcgatt ctgaccggta acgttggtat
    25741 taatggaggg aacagcggcg cgcgtgaagg ttcatacagc ttaccgtttg tccgtatgcc
    25801 gaccttggaa aacccgatcc agaccagcat ttcgatgttt atgtggaccg atgccattga
    25861 acgtggcccg gaaatgacgg cgctgcgtga tggtgtgcgc gggaaagata agctggatgt
    25921 gccgatcaaa atgatctgga actatgccgg taactgcctg attaaccagc attctgaaat
    25981 caaccgtacc catgaaatcc tccaggatga taagaagtgc gagctgattg tggttatcga
    26041 ctgccacatg acctcatcgg cgaaatatgc tgacatcctg ctgcctgact gcaccgcttc
    26101 cgaacagatg gactttgcac tggatgcatc ctgcgggaat atgtcttacg tgattttcaa
    26161 cgatcaggtg attaaaccgc gctttgaatg taagaccatc tatgaaatga ccagcgaact
    26221 ggcaaaacgt cttggcgttg agcaacagtt tactgaaggc cgtacccagg aagagtggat
    26281 gcggcatctg tatgcccagt cgcgggaagc gattcctgaa ctgccaacgt ttgaagagtt
    26341 ccgcaagcag gggatcttta aaaagcgcga cccacaaggg catcacgttg cttataaagc
    26401 cttccgtgaa gatccgcagg caaacccact gactacgcca tcgggcaaaa ttgagattta
    26461 ttcgcaggcg ctggctgaca ttgccgctac ctgggaattg ccagaaggcg atgtgatcga
    26521 tccactgccg atctacacgc cgggctttga aagttatcag gatccgctga acaaacagta
    26581 tccgctgcag cttacaggtt tccactataa atctcgcgtt cactcaactt acggcaacgt
    26641 tgatgtgctg aaagcggctt gccgtcagga aatgtggatc aacccgcttg atgcccaaaa
    26701 acgcggtatc cacaacggcg ataaagtcag gatctttaac gatcgtggtg aggttcatat
    26761 tgaggcgaaa gtgacgccac gaatgatgcc gggcgtggtc gcactgggtg aaggcgcctg
    26821 gtatgacccg gatgcaaaac gtgtcgataa gggcggttgt atcaacgtac tgaccactca
    26881 acgtccgtct cctctcgcta aggggaatcc gtcacataca aaccttgttc aggttgaaaa
    26941 ggtgtaagga gtaaccgatg acaacccagt atggattttt tattgattcc agccgttgca
    27001 ccggttgcaa aacctgcgag ctggcttgta aagactacaa agatttgacg ccagaagtca
    27061 gtttccgccg catttatgaa tatgctggcg gcgactggca ggaagataac ggtgtctggc
    27121 accagaacgt gtttgcctac tatctgtcga tttcgtgcaa ccactgcgaa gatccggctt
    27181 gtactaaagt ctgcccgagc ggtgcgatgc ataaacgcga agatggtttt gttgtggtcg
    27241 atgaagatgt atgcattggc tgccgttact gtcatatggc ttgcccgtat ggcgcaccgc
    27301 aatataacga aacgaaaggc catatgacca aatgcgatgg ttgttatgac cgtgttgcgg
    27361 agggtaaaaa gccgatctgt gttgaatctt gcccgctgcg ggcgctggat ttcggcccta
    27421 tcgacgagct gcgtaaaaaa catggcgatc tggcggccgt tgcgccgttg ccgcgagctc
    27481 actttaccaa accgaatatt gtgatcaaac ccaatgccaa tagccgcccg accggggata
    27541 ccactggcta tctggcaaac ccgaaggagg tgtgagatgg gaagtggatg gcatgaatgg
    27601 ccgctgatga tcttcacggt cttcgggcaa tgtgtggcgg gtggttttat cgttctggct
    27661 ttggcgctgc tcaaaggcga cctgcgagca gaagcccagc agcgtgttat cgcctgcatg
    27721 tttggcttat gggtactgat gggcattggc tttatcgcct ctatgctcca tcttggttca
    27781 ccgatgcgcg cctttaactc gctcaaccgg gtaggggctt cagcactcag taacgaaatc
    27841 gccagcggtt cgatcttttt tgctgtaggc ggcatcggct ggttgctggc aatgctgaaa
    27901 aagctgtcac cggcattgcg tacgctatgg ctggtggtga cgatggttct tggcgtcgtc
    27961 tttgtctgga tgatggtgcg tgtgtataac agcattgata ccgttccgac ctggtacagc
    28021 atctggacgc cgatgggctg ggcgatgcgt ctgctgccag ccatttctgt actggcactg
    28081 gtggtgagtg gcgtgatgtc agtgatgcag ggcacagagc tggcaactat tcatagttct
    28141 gtgcagcagg ccgcagcgct ggtgccggac tatggtgcgc tgatgtcctg gcggatcgtg
    28201 cttttggccg ttgccctgtg cttgtggatt gcaccacagc taaaaggtta tcagcctgcg
    28261 gttccgctac tatccgtctc attcattctg cttctggcag gggaattaat cggtcgcggc
    28321 gtattctatg gtttgcatat gaccgtgggg atggccgtcg caagctaatc agtaacaacc
    28381 ggggtttcgg ccccggttct cttttatttc tgcttcgtta acgtgtcata actggtcatc
    28441 aaattacgat aatcaggaat atggttagag aacaacgtcg ccagtccttc aatatcatta
    28501 cgccagtcac ggtgcagctc gcaagccacg ccgaaccatg tcatcaattg cgcaccagct
    28561 tgcgacatgc gatcccacgc cgaatgccgg gtaatttcat taaaggtccc tgatgcatca
    28621 gtcacgacaa agacatcaaa cccttcttca atggctgaaa gcgccgggaa tgccacgcaa
    28681 acttcggtta ccacaccggc aataattaac tgttttttac ctgtcgcttt gactgctttt
    28741 acaaaatctt cgttatccca ggcgttaata ttgcccgggc gagcaatgta aggtgcatcg
    28801 gggaattgtg ctttcagttc tagaactaat gggccgttag gaccggtttc aaaactggtg
    28861 gtaagaatag ttggtaagtt gaaatactta gccaggtcac ccagcgccag cacgttattt
    28921 ttaaacttat cgggttcgat atcccgtaca agggaaagta aaccagcctg gtgatcaaca
    28981 agcaaaacgg cagcatcatt tttatcaaga cgaacatacg gtttggtcat cctcttctcc
    29041 tttcgagagt cagtggtacg tctgaaatcg gtcagacgcg atgcattgct ctgaaagcat
    29101 agacgggaaa tatgagtttg ctgtgaccat gaaatttttc gactgaacga agcgtatgaa
    29161 atttgtgtta gatcaataaa aacaatcaga tgagattaat catctcttca gcattagcgt
    29221 tatgaatgag taatataaat cgataatacc gccgctgcga tatgcgcgta ttttcaccca
    29281 ttgacaatgt ttttggcggt ggcatgatgc gcatgaaatt tgaacttcct cacggtttta
    29341 attcatgtcc acgtataccc ggcctgtcat gcttttgctg tctggcctgc ttttgttgac
    29401 tctggcgatc gcggtgttaa atacactcgt gccgctttgg ctcgcccagg aacacatgtc
    29461 cacatggcag gtaggcgttg tcagctcatc ctattttacc ggcaaccttg tcggtacatt
    29521 gctgacaggg tatgtcatta aacgcattgg ctttaaccgc agctattatc tgtcctcctt
    29581 catttttgct gctggctgtg ccggccttgg cctgatgatt ggtttctgga gctggttggc
    29641 gtggcgtttt gtcgcgggcg tcggctgtgc catgatttgg gtggttgttg agagcgcgct
    29701 gatgtgcagt gggacgtcac gtaaccgtgg gcgtttgctt gctgcgtata tgatggttta
    29761 ttacgtggga acgtttttag gccagttact ggtcagcaaa gtctcaaccg agctgatgag
    29821 cgtattgccg tgggttacag gtttgacatt ggcagggatc ttaccgctgt tgtttacgcg
    29881 tgtcctgaat cagcaggctg aaaaccatga ttcgacgtca attacggcaa tgctaaaact
    29941 ccgtcaggcg cggcttggcg tgaatggc
//
LOCUS       DAJTUP010000039        29805 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTUP010000039 DAJTUP010000000
VERSION     DAJTUP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29805
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.039"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            9..1382
                     /gene="ravA"
                     /locus_tag="OO468_001842"
     CDS             9..1382
                     /gene="ravA"
                     /locus_tag="OO468_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001453595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR6258980.1"
                     /translation="MFLLGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGP
                     LSIQALKDEGRYERLTSGYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVE
                     KIPMRLLVAASNELPEADSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPV
                     PDALQVTDEEYERWQKEIGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRL
                     LQASAFFSGRSAVAPVDLILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLG
                     AIVQRHLQLQQQQSDKTALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDM
                     EVVHISFERSALEQWLSKGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLA
                     LPGSSAEGLPGEIKQQLEELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQ
                     IRQAQQC"
     gene            1376..2827
                     /gene="viaA"
                     /locus_tag="OO468_001843"
     CDS             1376..2827
                     /gene="viaA"
                     /locus_tag="OO468_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR6258981.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(2832..3824)
                     /gene="asnA"
                     /locus_tag="OO468_001844"
     CDS             complement(2832..3824)
                     /gene="asnA"
                     /locus_tag="OO468_001844"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR6258982.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            3976..4434
                     /gene="asnC"
                     /locus_tag="OO468_001845"
     CDS             3976..4434
                     /gene="asnC"
                     /locus_tag="OO468_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR6258983.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4524..4967
                     /gene="mioC"
                     /locus_tag="OO468_001846"
     CDS             4524..4967
                     /gene="mioC"
                     /locus_tag="OO468_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR6258984.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5346..7235
                     /gene="mnmG"
                     /locus_tag="OO468_001847"
     CDS             5346..7235
                     /gene="mnmG"
                     /locus_tag="OO468_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR6258985.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7299..7922
                     /gene="rsmG"
                     /locus_tag="OO468_001848"
     CDS             7299..7922
                     /gene="rsmG"
                     /locus_tag="OO468_001848"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR6258986.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8539..8919
                     /gene="atpI"
                     /locus_tag="OO468_001849"
     CDS             8539..8919
                     /gene="atpI"
                     /locus_tag="OO468_001849"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR6258987.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            8928..9743
                     /gene="atpB"
                     /locus_tag="OO468_001850"
     CDS             8928..9743
                     /gene="atpB"
                     /locus_tag="OO468_001850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR6258988.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9790..10029
                     /gene="atpE"
                     /locus_tag="OO468_001851"
     CDS             9790..10029
                     /gene="atpE"
                     /locus_tag="OO468_001851"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR6258989.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10091..10561
                     /gene="atpF"
                     /locus_tag="OO468_001852"
     CDS             10091..10561
                     /gene="atpF"
                     /locus_tag="OO468_001852"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR6258990.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10576..11109
                     /gene="atpH"
                     /locus_tag="OO468_001853"
     CDS             10576..11109
                     /gene="atpH"
                     /locus_tag="OO468_001853"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR6258991.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11122..12663
                     /gene="atpA"
                     /locus_tag="OO468_001854"
     CDS             11122..12663
                     /gene="atpA"
                     /locus_tag="OO468_001854"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR6258992.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12714..13577
                     /gene="atpG"
                     /locus_tag="OO468_001855"
     CDS             12714..13577
                     /gene="atpG"
                     /locus_tag="OO468_001855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR6258993.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13604..14986
                     /gene="atpD"
                     /locus_tag="OO468_001856"
     CDS             13604..14986
                     /gene="atpD"
                     /locus_tag="OO468_001856"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR6258994.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15007..15426
                     /gene="atpC"
                     /locus_tag="OO468_001857"
     CDS             15007..15426
                     /gene="atpC"
                     /locus_tag="OO468_001857"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR6258995.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15777..17147
                     /gene="glmU"
                     /locus_tag="OO468_001858"
     CDS             15777..17147
                     /gene="glmU"
                     /locus_tag="OO468_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR6258996.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17309..19138
                     /gene="glmS"
                     /locus_tag="OO468_001859"
     CDS             17309..19138
                     /gene="glmS"
                     /locus_tag="OO468_001859"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR6258997.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19300..20121
                     /locus_tag="OO468_001860"
     CDS             19300..20121
                     /locus_tag="OO468_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR6258998.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20108..22216
                     /locus_tag="OO468_001861"
     CDS             20108..22216
                     /locus_tag="OO468_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6258999.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22213..23880
                     /locus_tag="OO468_001862"
     CDS             22213..23880
                     /locus_tag="OO468_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6259000.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            23883..25409
                     /locus_tag="OO468_001863"
     CDS             23883..25409
                     /locus_tag="OO468_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR6259001.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25410..27026
                     /locus_tag="OO468_001864"
     CDS             25410..27026
                     /locus_tag="OO468_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR6259002.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27082..27234)
                     /locus_tag="OO468_001865"
                     /pseudo
     CDS             complement(27082..27234)
                     /locus_tag="OO468_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27257..27634)
                     /locus_tag="OO468_001866"
     CDS             complement(27257..27634)
                     /locus_tag="OO468_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259003.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28044..28415)
                     /locus_tag="OO468_001867"
     CDS             complement(28044..28415)
                     /locus_tag="OO468_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259004.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28476..28973)
                     /locus_tag="OO468_001868"
     CDS             complement(28476..28973)
                     /locus_tag="OO468_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259005.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29049..>29805)
                     /locus_tag="OO468_001869"
     CDS             complement(29049..>29805)
                     /locus_tag="OO468_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /protein_id="HCR6259006.1"
                     /translation="QLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPHSDIDLLVTVT
                     VRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAKRELQFGEWQ
                     RNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLFEALNETLT
                     LWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQPVILEARQ
                     AYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         7673 a         6991 c         7677 g         7464 t
ORIGIN      
        1 gtgaaagtgt gttcctcctt ggcccgccag gtattgccaa aagtttgatc gcccggcgct
       61 taaaattcgc ctttcagaat gcccgcgcgt ttgaatatct gatgacccgc ttctccacgc
      121 cggaagaagt ttttggtccc ctttctattc aggcgctaaa agatgaaggg cgctatgaac
      181 gtttaaccag cggttacctg ccggaagcag aaatcgtctt tctggatgag atctggaaag
      241 cgggcccggc aattcttaat accttgctca ccgccattaa cgagcgccag ttccgcaacg
      301 gtgcacacgt agaaaaaatc ccgatgcgtc tactggtggc ggcctccaac gagctgccgg
      361 aagcagacag cagtctggaa gcgttatatg accgcatgct gattcgtcta tggttagata
      421 aagtgcaaga taaagcgaat ttccgctcca tgctgaccag tcaacaggat gaaaacgaca
      481 atccggttcc tgatgccttg caggtcacag atgaagaata tgaacgctgg cagaaagaga
      541 ttggtgaaat tacgctgccc gatcatgtat ttgagctgat ttttattctg cgccagcaac
      601 tggataaatt accggatgcg ccttatgtct cggatcgtcg ctggaaaaaa gcgatccgct
      661 tattgcaggc cagcgccttt tttagcggtc gcagtgctgt tgccccggtt gatctcattt
      721 tgctgaaaga ttgcctgtgg tatgacgcgc aaagcctgaa tttgatacaa caacaaattg
      781 atgtattgat gaccggtcat gcctggcaac agcaagggat gttgacccgc ctgggcgcga
      841 ttgtgcaacg tcacctgcaa ctccagcagc aacaaagcga taaaacagcc ttaacgttaa
      901 ttcgtctggg cggcattttc agccgtcgtc agcagtatca acttcctgtt aacgttactg
      961 cttccactct gactctgctg ctgcaaaaac cgttaaaact gcatgatatg gaagtggttc
     1021 atatctcctt tgagcgtagc gcgctggaac agtggctgag taaaggtggt gaaattcgcg
     1081 gcaaactgaa cggtatcggc tttgcccaga aactgaatct ggaagttgat agcacccaac
     1141 atcttgttgt acgcgatgta agtttacaag gcagtacgct ggcacttccc ggttcatcgg
     1201 ctgaaggtct gccaggtgaa ataaaacaac aactggaaga gcttgaaagc gactggcgta
     1261 agcaacacgc tttattcagc gaacagcaaa aatgtctgtt tatccctggc gactggttag
     1321 gtcgcattga agacagccta caggatgtcg gtgcacagat tcgtcaggca caacaatgct
     1381 aacgctggat acgcttaatg tgatgctggc cgtcagcgaa gagggattga tcgaagagat
     1441 gatcatcgca ctgctggcct caccgcagct ggcggtcttc tttgaaaaat tcccacgact
     1501 gaaggcggca atcactgatg atgttccccg ctggcgtgag gcgctgcgca gtcggctgaa
     1561 agatgcccga gtcccgccgg aactcaccga agaggtgatg tgctatcagc aaagccagct
     1621 cctctccacg ccgcagttta ttgtgcagct accacagatc ctggacttac tgcatcgtct
     1681 gaattcccca tgggcagaac aagcccggca gttggttgat gctaacagca caataacttc
     1741 agcgttacac acgctttttc tccagcgttg gcgtttaagt ctgatcgtgc aagcaacgac
     1801 gttaaatcaa cagctattag aagaagaacg cgaacaactg ttaagtgaag ttcaggaacg
     1861 catgacgctg agcggacaac ttgaaccgat tctcgcagat aacaataccg cagctggtcg
     1921 tctgtgggat atgagcgccg gtcaacttaa acgtggcgac tatcagttta ttgtgaaata
     1981 cggtgaattt cttaacgaac agccggaact gaaacgcctg gcagagcagc tggggcgttc
     2041 tcgggaagcc aaatcaatac cgcgcaacga tgcgcagatg gaaaccttcc gcaccatggt
     2101 gcgcgaaccg gcgacggttc ctgagcaggt tgatggtctg caacaaagcg atgatatttt
     2161 acgtctcctg ccgccagaac tggcgacact agggataacg gaactggagt atgagtttta
     2221 ccgtcggctg gtggaaaaac agttgctcac ctatcgcctg cacggtgagt cgtggcgtga
     2281 aaaagtgttc gaacgtccgg tggtacataa agattacgat gaacagccgc gcgggccgtt
     2341 tattgtctgt gtggatactt ccggctcaat gggcggcttt aatgaacagt gtgcgaaagc
     2401 gttctgcctg gctttgatgc gcattgctct cgctgaaaac cggcgctgtt atattatgct
     2461 attttccacc gagatcgtcc gttatgagct ttcaggccca caaggcatcg aacaagcaat
     2521 ccgtttttta agccagcagt ttcgtggcgg caccgatctt gccagttgtt ttcgcgccat
     2581 tatggaacgc ttgcaaagca gggaatggtt tgatgccgat gcggtggtga tttctgattt
     2641 tatcgctcag cggttgcctg acgacgtgac gagtaaagtg aaagagctgc agcgggtaca
     2701 tcagcatcgc tttcatgccg tggcgatgtc ggcacacggc aaacccggca tcatgcgcat
     2761 tttcgatcat atctggcgct ttgataccgg gatgcgaagc cgcctgctca gacgctggcg
     2821 gcgataaatt attacagcag agaagggacg ctctcgcgaa cagcagctgg ccatactcca
     2881 cactgaacct ggccgatatg cggcagttgc agcagcagca tagtcaaacg agactggccg
     2941 ataccgccgc cgatggtctg cggcatttca ccgcgcagca gcgcctgatg ccattccagc
     3001 tgcagacgat cttcatcacc ggtcagcgcc agctgatgct tcagcgtgtc ggcatccacg
     3061 cggatcccca tggaagaaag ctcaaacgca tcttccagta ccgggttcca caccagaata
     3121 tcgccgttca ggcccgcatg gcccagctct gacggggtgc tccagtcatc ataatccggt
     3181 gcgcgcacgt cgtgacgatg accatcactc aacttgccgc caatcccgac gaggaatacc
     3241 gcgccaagat ctttcgcaat cgcccgctca cgccctttgg catcgagatc cggataacga
     3301 gacagtaact cctggctgtg tacgaagtga atctgatccg gcaggaacgg tgccaggcca
     3361 aactcttcgt taaccgcagc ttcggttgct ttaattcccg cccagatcgc ctctaccgtg
     3421 cttttcagag tcgagagttg acgctcaccg tcgcccatta cgcgttccca gtcccactgg
     3481 tcaacataga ccgagtgcaa cggagaaaga cggtcttcat cggggcgaag ggctttcatg
     3541 tgcgtataca gcccttcgcc cgcgctgaag tcgtgttgcc ctaaggtctg acgtttccac
     3601 ttcgccagtg aatgaaccac ttcgaactgg gcatcaggca gagctttcac ttttacctgc
     3661 accgcttttt cacagcccga caagttatcc tgcgtgccat cccccacacg gctaagaatc
     3721 ggcgcctgga cttcgatcag ccccagacgt tcttccagtt ggcgagaaaa gtgagatttc
     3781 acgaagctaa tttggcgttg tttggcaatg taagcggttt tcatttttta tactcctgcg
     3841 tcctgttgct tatgattaag caacaaaaac cgacacacat gcaataatca ttcaataaaa
     3901 agcctaacac acttttgatt cattaaaaaa gacggctaaa atagaatgaa tcatcaatct
     3961 gcataagaaa atcctatgga aaattatctg atcgacaatc tggaccgtgg catcctggaa
     4021 gcattaatgg gcaatgcgcg caccgcttac gccgaactgg cgaaacaatt tggcgtcagt
     4081 ccggggacga ttcacgttcg agtagagaaa atgaagcagg cggggatcat taccggggcg
     4141 cgtattgatg tcagcccgaa gcagctcggt tatgacgtag gctgctttat cggcattata
     4201 ttaaagagcg ccaaagacta cccttccgcg ctggcaaagc tggaaagcct tgatgaagtc
     4261 actgaagcct attacacaac cggccactac agcatcttta taaaagttat gtgccgttcg
     4321 atcgacgctc tccagcatgt acttatcaac aagatccaaa caattgatga aattcagtcc
     4381 accgagacat tgatcgtcct gcaaaacccg atcatgcgta ccatcaagcc ctgatcggct
     4441 tttttaatcc catacttttc cacaggtaga tcccaacgcg ttcacagcgt acaatacgcc
     4501 actcttaata aaggtggcgg tttatggcag atatcactct tatcagcggc agcaccctcg
     4561 gcggtgccga atatgtagca gaacacctgg ctgaaaagct ggaagaggtg ggttttacca
     4621 ccgaaacgct gcacggtccg ctgttggaag atttatctgc ctcggggatc tggctggtta
     4681 tcagctccac ccacggtgcc ggagatattc cggacaacct ttctcctttc tatgaagcat
     4741 tgcaggaaca gaagcccgat ctttctgcgg tccgctttgg cacaatcggt attggcagtc
     4801 gtgaatatga caccttttgt ggggctatcg ataaactcga ggcagaactc aaaaattccg
     4861 gtgcaaaaca gacaggcgaa acactgaaga tcaacattct tgatcacgac attccggaag
     4921 atccggcaga agaatggctg ggatcgtgga ttaatttact caaataagta tacagatcgt
     4981 gcgatctact gtggataact ctgtcaggaa gcttggatca accggtagct atccaaagaa
     5041 caaccgttgt tcagtttttg agttgtgtat aacccctcat tctgatccca gcttatacgg
     5101 tccaggatca ccgatcattc acagttaatg atcctttcca ggttgttgat cttaaaaatc
     5161 ggatccttgt tatccacagg gcagtgcgat cctaataaga gatcacaata gaacagatct
     5221 ctaaataaat agatcttctt tttaataccc aggatcccag gtctttctca agccgacaaa
     5281 gttgagtaga atccacggcc cgggcttcaa tccattttca taccgcgtta tgcgaggcaa
     5341 tcaccatgtt ttatccggat ccttttgacg tcatcatcat tggcgggggt catgcaggca
     5401 ccgaggccgc gatggctgcg gcgcgtatgg gtcaacagac tctgcttttg acacacaata
     5461 tcgacactct ggggcagatg agctgcaacc cggcgatcgg cggtattggg aagggacatc
     5521 tggtaaaaga agtggatgca ctcggcggtc tgatggcgaa agcgatcgat caagcgggta
     5581 tccagtttag gatactaaac gcaagcaaag gaccggcggt tcgcgctacc cgagctcagg
     5641 cggatcgtgt gcgctaccgt caggcggtac gtacggcgct ggagaaccaa ccgaacctga
     5701 tgatcttcca gcaggcggtt gaagatctta ttgtcgaaaa cgatcgcgtg gtcggtgctg
     5761 ttacccaaat gggactgaag ttccgtgcca aagctgtggt gctcaccgtt gggacgttcc
     5821 tcgacggcaa aattcatatc ggtctggata attacagcgg tggccgtgct ggcgatccgc
     5881 catccattcc gctctctcgc cgtttgcgtg aactgccgct gcgcgttggt cgtctgaaaa
     5941 ccggtactcc gccgcgtatt gatgctcgaa ccatcgactt tagcgtgctg gcgcaacagc
     6001 atggcgataa cccaatgccg gtattctcgt ttatgggcaa tgcgtccctg catccccagc
     6061 aggtgccgtg ttatatcact cataccaacg agaaaaccca tgatgtgatc cgcagtaacc
     6121 tcgatcgtag cccaatgtat gcaggggtga tcgaaggtgt cggcccacgc tactgcccgt
     6181 cgatcgaaga caaagtcatg cgcttcgcag acagaaatca gcatcagatc ttccttgaac
     6241 cggaaggact gacctctaat gaaatttatc cgaacggtat ctccaccagc ctgccgttcg
     6301 atgtgcagat gcaaatcgtc cgctctatgc aggggatgga aaacgcgaag atcgtgcgtc
     6361 cgggttatgc cattgagtat gacttcttcg atcctcgcga cctgaaaccg acgctggaga
     6421 gcaagtttat ccaggggctg ttctttgctg gtcagattaa cggcactacc ggttacgaag
     6481 aagccgctgc gcaaggtttg ctggccggtc ttaacgctgc ccgtctgtct gctgacaaag
     6541 aaggttgggc tccggcacgt tctcaggcgt atctcggcgt actggttgat gacctgtgca
     6601 ctttaggaac caaagaaccg tatcgtatgt ttacttcgcg cgcagaatat cgtctgatgc
     6661 tgcgcgaaga taatgcggat ctgcgtttga ctgaaatcgg tcgtgaactg ggcctggtgg
     6721 atgacgaacg ttgggcgcgc tttaacgaga aacttgagaa tatcgagcgt gagcgtcagc
     6781 gtctgaaatc gacctgggta accccgtcgg cggaagctgc agccgaagtg aatgctcacc
     6841 tgactgcgcc gctttcccgt gaagccagtg gtgaagatct gctgcgtcgt ccggaaatga
     6901 cttatgaaaa attaaccacg ctgacgccgt ttgcccctgc gttgacagac gaacaggcgg
     6961 cggaacaggt tgagattcag gttaaatacg aaggttatat cgcgcgccag caagatgaga
     7021 tcgaaaagca gctgcgtaac gagaacaccc tgctacccgc gacactggat taccgccaga
     7081 tatccggtct ttctaacgaa gtgatcgcca aacttaacga tcacaaaccg gcctctatcg
     7141 gccaggcttc gcgtatttct ggcgtcacgc ctgcggccat ctccattctg ctggtgtggc
     7201 tgaaaaaaca gggtatgctg cgtcgtagcg cataacgcat taaaaatgcc tggtaagcac
     7261 ccgcttacca ggcaacgcat caagaacagg taatcaccgt gctcaacaaa ctctccttac
     7321 tgctgaaaga cgcaggtatt tcgcttactg atcaccagaa aaaccagctt attgcctacg
     7381 tgaatatgct gcataaatgg aacaaagcgt acaacctgac ttcggtccgc gatcctaatg
     7441 agatgctggt acgccatatt ctcgatagca ttgtggtggc accgtatctg caaggtgaac
     7501 ggtttatcga tgtcggcacc ggaccaggac tgccaggcat tccactctct atcgtgcgtc
     7561 ctgaagccca tttcactctg ttggatagcc ttggtaaacg cgtgcgtttc cttcgtcagg
     7621 tgcaacatga gcttaaactg gagaatattg aaccagtaca gagcagggta gaagagtttc
     7681 cttcagagcc gccatttgat ggcgtaatta gccgcgcttt tgcctctctg aacgatatgg
     7741 tgagctggtg ccaccatctt cctggtgagc aaggccgttt ctacgcgctg aaagggcaaa
     7801 tgccggaaga tgaaatcgct ttgttgcccg aagaatatca ggtcgaatca gtggttaaac
     7861 ttcaggttcc agccctggat ggcgaacgtc atctggtggt gattaaagca aataaaattt
     7921 aatttttatc aaaaaaatca taaaaaattg accggttaga ctgttaacaa caaccaggtt
     7981 ttctactgat ataactggtt acatttaacg ccacgttcac tcttttgcat caacaagata
     8041 acgtggcttt ttttggtaag cagaaaataa gtcattagtg aaaatatcag tctgctaaaa
     8101 atcggcgcta agaaccatca ttggctgtta aaacattatt aaaaatgtca atgagtggtt
     8161 tttgttgtgt aaatgttatt tattaaaaca gtatctgttt ttagactgaa atatcataaa
     8221 cttgtaaagg catcatttgc caagtaaata aatatgctgt gcgcgaacat gcgcaatatg
     8281 tgatctgaag cacgctttat caccagtgtt tacgcgttat ttacagtttt tcatgatcga
     8341 acaggggtag cggaaaagtc gcaattgtat gcactggaaa aatatttaaa catttattca
     8401 ccttttggct acttattgtt tgaaatcacg ggggcgcacc gtataatttg accgcttttt
     8461 gatgcttgac tctaagcctt aaagaaagtt ttatacgaca cgcggcatac ctcgaaggga
     8521 gcaggagtga aaaacgtgat gtctgtgtcg ctcgtgagtc gaaacgttgc tcggaagctt
     8581 ctgctcgttc agttactggt ggtgatagca agtggattgc tgttcagcct caaagacccc
     8641 ttctggggcg tctctgcaat aagcgggggc ctggcagtct ttctgcctaa cgttttgttt
     8701 atgatatttg cctggcgtca ccaggcgcat acaccagcga aaggccgggt ggcttggaca
     8761 ttcgcatttg gcgaagcttt caaagttctg gcgatgttgg tgttactggt ggtggcgttg
     8821 gcggttttaa aggcggtatt cttgccgctg atcgttacgt gggttttggt gctggtggtt
     8881 cagatactgg caccggctgt aattaacaac aaagggtaaa aggcatcatg gcttcagaaa
     8941 atatgacgcc gcaggattac ataggacacc acctgaataa ccttcagctg gacctgcgta
     9001 cattctcgct ggtggatcca caaaaccccc cagccacctt ctggacaatc aatattgact
     9061 ccatgttctt ctcggtggtg ctgggtctgt tgttcctggt tttattccgt agcgtagcca
     9121 aaaaggcgac cagcggtgtg ccaggtaagt ttcagaccgc gattgagctg gtgatcggct
     9181 ttgttaatgg tagcgtgaaa gacatgtacc atggcaaaag caagctgatt gctccgctgg
     9241 ccctgacgat cttcgtctgg gtattcctga tgaatctgat ggatttactg cctatcgact
     9301 tgctgccgta cattgctgaa catgtactgg gtctgcctgc actgcgtgtg gttccgtctg
     9361 cggacgtgaa cgtaacgctg tctatggcac tgggcgtatt tatcctgatt ctgttctaca
     9421 acatcaaaat gaaaggcatc ggcggcttca cgaaagagtt gacgctgcag ccgttcaatc
     9481 actgggcgtt cattcctgtc aacttaatcc ttgaaggggt aagcctgctg tccaaaccag
     9541 tttcactcgg tttgcgactg ttcggtaaca tgtatgccgg tgagctgatt ttcattctga
     9601 ttgctggtct gttgccgtgg tggtcacagt ggatcctgaa tgtgccgtgg gccattttcc
     9661 acatcctgat cattacgctg caagccttca tcttcatggt tctgacgatc gtctatctgt
     9721 cgatggcgtc tgaagaacat taatttacca acactactac gttttaactg aaacaaactg
     9781 gagactgtca tggaaaacct gaatatggat ctgctgtaca tggctgccgc tgtgatgatg
     9841 ggtctggcgg caatcggtgc tgcgatcggt atcggcatcc tcgggggtaa attcctggaa
     9901 ggcgcagcgc gtcaacctga tctgattcct ctgctgcgta ctcagttctt tatcgttatg
     9961 ggtctggtgg atgctatccc gatgatcgct gtaggtctgg gtctgtacgt gatgttcgct
    10021 gtcgcgtagt aagcgttgct tttatttaaa gagcaatatc agaacgttaa ctaaatagag
    10081 gcattgtgct gtgaatctta acgcaacaat cctcggccag gccatcgcgt ttgtcctgtt
    10141 cgttctgttc tgcatgaagt acgtatggcc gccattaatg gcagccatcg aaaaacgtca
    10201 aaaagaaatt gctgacggcc ttgcttccgc agaacgagca cataaggacc ttgaccttgc
    10261 aaaggccagc gcgaccgacc agctgaaaaa agcgaaagcg gaagcccagg taatcatcga
    10321 gcaggcgaac aaacgccgct cgcagattct ggacgaagcg aaagctgagg cagaacagga
    10381 acgtactaaa atcgtggccc aggcgcaggc agaaattgaa gccgagcgta aacgtgcccg
    10441 tgaagagctg cgtaagcaag ttgctatcct ggctgttgct ggcgccgaga agatcatcga
    10501 acgttccgtg gatgaagctg ctaacagcga catcgtggat aaacttgtcg ctgaactgta
    10561 aggagggagg ggctgatgtc tgaatttatt acggtagctc gcccctacgc caaagcagct
    10621 tttgactttg ccgtcgaaca ccaaagtgta gaacgctggc aggacatgct gacgtttgcc
    10681 gccgaggtaa ccaaaaacga acaaatggca gagcttctct ctggcgcgct tgcgccagaa
    10741 acgctcgccg agtcgtttat cgcagtttgt ggtgagcaac tggacgaaaa cggtcagaac
    10801 ctgattcggg taatggctga aaatggtcgt cttaacgcgc tcccggatgt tctggagcag
    10861 tttattcacc ttcgtgccgt gagtgaggct accgctgagg tagacgtcat ttccgctgcc
    10921 gcactgagtg aacaacagct cgcgaaaatt tctgctgcga tggaaaaacg tctgtcacgc
    10981 aaagttaagc tgaattgcaa aatcgataag tctgtaatgg ctggcgttat catccgatcg
    11041 ggtgatatgg tcattgatgg cagcgtacgc ggtcgtcttg agcgccttgc agacgtcttg
    11101 cagtcttaag gggactggag catgcaactg aattccaccg aaatcagcga actgatcaag
    11161 cagcgcattg ctcagttcaa tgttgtgagt gaagctcaca acgaaggtac tattgtttct
    11221 gtaagtgacg gtgttatccg cattcacggc ctggccgatt gtatgcaggg tgaaatgatc
    11281 tccctgccgg gtaaccgtta cgctatcgca ctgaacctcg agcgcgactc tgtaggtgcg
    11341 gttgttatgg gtccgtacgc tgaccttgcc gaaggcatga aagttaagtg tactggccgt
    11401 atcctggaag ttccggttgg ccgtggcctg ctgggccgtg tggttaacac tctgggtgca
    11461 ccaatcgacg gtaaaggtcc gctggatcac gacggcttct ctgctgtaga agcaatcgct
    11521 ccgggcgtta tcgaacgtca gtccgtagat cagccggtac agaccggtta taaagccgtt
    11581 gactccatga tcccaatcgg tcgtggtcag cgtgaattga tcatcggtga ccgtcagaca
    11641 ggtaaaaccg cactggctat cgatgccatc atcaaccagc gcgattccgg tatcaaatgt
    11701 atctatgtcg ctatcggcca gaaagcgtcc accatttcta acgtggtacg taaactggaa
    11761 gagcacggcg cactggctaa caccatcgtt gtggtagcaa ccgcgtctga atccgctgca
    11821 ctgcaatacc tggcaccgta tgccggttgc gcaatgggcg aatacttccg tgaccgcggt
    11881 gaagatgcgc tgatcattta cgatgacctg tctaaacagg ctgttgctta ccgtcagatc
    11941 tccctgctgc tccgtcgtcc gccaggacgt gaagcattcc cgggcgacgt tttctacctc
    12001 cactctcgtc tgctggagcg tgctgcacgt gttaacgccg aatacgttga agccttcacc
    12061 aaaggtgaag tgaaagggaa aaccggttct ctgaccgcac tgccgattat cgaaactcag
    12121 gcgggtgacg tttctgcgtt cgttccgacc aacgtaatct ccattaccga tggtcagatc
    12181 ttcctggaaa ccaacctgtt caacgccggt attcgtcctg cggttaaccc gggtatttcc
    12241 gtatcccgtg ttggtggtgc agcacagacc aagatcatga aaaaactgtc cggtggtatc
    12301 cgtaccgctc tggcacagta tcgtgaactg gcagcgttct ctcagtttgc atccgacctt
    12361 gacgatgcaa cacgtaagca gcttgaccac ggtcagaaag tgaccgaact gctgaaacag
    12421 aaacagtatg cgccgatgtc cgttgcgcag cagtctctgg ttctgttcgc agcagaacgt
    12481 ggttacctgg cggatgttga actgtcgaaa attggcagct tcgaagccgc tctgctggct
    12541 tacgtcgacc gtgatcacgc tccgttgatg caagagatca accagaccgg tggctacaac
    12601 gacgaaatcg aaggcaagct gaaaggcatc ctcgattcct tcaaagcaac ccaatcctgg
    12661 taacgtctgg aggcttgcct tagggcaggc cgcaaggcat tgaggagaag ctcatggccg
    12721 gcgcaaaaga gatacgtagt aagatcgcaa gcgtccagaa cacgcaaaag atcactaaag
    12781 cgatggagat ggtcgccgct tccaaaatgc gtaaatcgca ggatcgcatg gcggccagcc
    12841 gtccttatgc agaaaccatg cgcaaagtga ttggtcacct tgcacacggt aatctggaat
    12901 ataagcaccc ttacctggaa gaccgcgacg ttaaacgcgt gggctacctg gtggtgtcga
    12961 ccgaccgtgg tttgtgcggt ggtttgaaca ttaacctgtt caaaaaactg ctggcggaaa
    13021 tgaagacctg gaccgacaaa ggcgttcaat gcgacctcgc aatgatcggc tcgaaaggcg
    13081 tgtcgttctt caactccgtg ggcggcaatg ttgttgccca ggtcaccggc atgggggata
    13141 acccttccct gtccgaactg atcggtccgg taaaagtgat gttgcaggcc tacgacgaag
    13201 gccgtctgga caagctttac attgtcagca acaaatttat taacaccatg tctcaggttc
    13261 cgaccatcag ccagctgctg ccgttaccgg catcagatga tgatgatctg aaacataaat
    13321 cctgggatta cctgtacgaa cccgatccga aggcgttgct ggataccctg ctgcgtcgtt
    13381 atgtcgaatc tcaggtttat cagggcgtgg ttgaaaacct ggccagcgag caggccgccc
    13441 gtatggtggc gatgaaagcc gcgaccgaca atggcggcag cctgattaaa gagctgcagt
    13501 tggtatacaa caaagctcgt caggccagca ttactcagga actcaccgag atcgtctcgg
    13561 gggccgccgc ggtttaaaca ggttatttcg tagaggattt aagatggcta ctggaaagat
    13621 tgtccaggta atcggcgccg tagttgacgt cgaattccct caggatgccg taccgcgcgt
    13681 gtacgatgct cttgaggtgc aaaatggtaa tgagcgtctg gtgctggaag ttcagcagca
    13741 gctcggcggc ggtatcgtgc gtaccatcgc aatgggttcc tccgacggtc tgcgtcgcgg
    13801 tctggatgta aaagacctcg aacacccgat cgaagtcccg gtaggtaaag cgactctggg
    13861 ccgtatcatg aacgtactgg gtgaaccggt cgacatgaaa ggcgagatcg gtgaagaaga
    13921 gcgttgggcg attcaccgcg cagcaccttc ctacgaagag ctgtcaaact ctcaggaact
    13981 gctggaaacc ggtatcaaag ttatcgacct gatgtgtccg ttcgctaagg gcggtaaagt
    14041 tggtctgttc ggtggtgcgg gtgtaggtaa aaccgtaaac atgatggagc tcattcgtaa
    14101 catcgcgatc gagcactccg gttactctgt gtttgcgggc gtaggtgaac gtactcgtga
    14161 gggtaacgac ttctaccacg aaatgaccga ctccaacgtt atcgacaaag tatccctggt
    14221 gtatggccag atgaacgagc cgccgggaaa ccgtctgcgc gttgctctga ccggtctgac
    14281 catggctgag aaattccgtg acgaaggtcg tgacgttctg ctgttcgttg acaacatcta
    14341 tcgttacacc ctggccggta cggaagtatc cgcactgctg ggccgtatgc cttcagcggt
    14401 aggttatcag ccgaccctgg cggaagagat gggcgttctg caggaacgta tcacctccac
    14461 caaaactggt tctatcacct ccgtacaggc ggtatacgta cctgcggatg acttgactga
    14521 cccgtctccg gcaaccacct ttgcgcacct tgacgcaacc gtggtactga gccgtcagat
    14581 cgcgtctctg ggtatctacc cggccgttga cccgctggac tccaccagcc gtcagctgga
    14641 cccgctggtg gttggtcagg aacactacga caccgcgcgt ggcgttcagt ccatcctgca
    14701 acgttatcag gaactgaaag acatcatcgc catcctgggt atggatgaac tgtctgaaga
    14761 agacaaactg gtggtagcgc gtgctcgtaa gatccagcgc ttcctgtccc agccgttctt
    14821 cgtggcagaa gtattcaccg gttctccggg taaatacgtc tccctgaaag acaccatccg
    14881 tggctttaaa ggcatcatgg aaggcgaata cgatcacctg ccggagcagg cgttctacat
    14941 ggtcggttcc atcgaagaag ctgtggaaaa agccaaaaaa ctttaacgcc ttaatcggag
    15001 ggtgatatgg caatgactta ccacctggac gtcgtcagcg cagagcaaca aatgttctct
    15061 ggtctggtcg agaaaatcca ggtaacgggt agcgaaggtg aactggggat ctaccctggc
    15121 cacgcaccgc tgctcaccgc cattaagcct ggtatgattc gcatcgtgaa acagcacggt
    15181 cacgaagagt ttatctatct gtctggcggc attcttgaag tgcagcctgg caacgtgacc
    15241 gttctggccg acaccgcaat tcgcggccag gatctcgacg aagcgcgagc catggaagcg
    15301 aaacgtaagg ctgaagagca cattagcagc tctcacggcg acgtagatta cgctcaggcg
    15361 tctgcggaac tggccaaagc gatcgcgcag ctgcgcgtta tcgagttgac caaaaaagcg
    15421 atgtaacacc ggcttgaaaa gcacaaaagc cagtctggaa acaggctggc tttttttacg
    15481 cgttgacccg tcctgaagag cgttcacata gatcctgctg atataaaaca cccctgtttt
    15541 cccgtttatt cattgatcga aataagagca aaaacatcca cctgacgctt aaatgaaggt
    15601 actgccttaa ttttctgcag ataaaaggcg tgacgatggt cgaaaatggc actttcgtca
    15661 gcggggataa tccgttattg aacaatttat cctctgtcca tttcacgatg aaaaaaatgt
    15721 agttttttca aggtgaagcg gtttaaattc gttctcaaat tacagtcagg acgcgtatgt
    15781 tgaataatgc tatgagcgta gtgatccttg ccgcaggcaa aggcacgcgc atgtattccg
    15841 atcttccgaa agtgctgcat acccttgccg ggaaagcgat ggttcagcat gtcattgatg
    15901 ctgcgaatga attaggcgca gcgcacgttc acctggtgta cggtcacggc ggcgatctgc
    15961 ttaaacaggc gctgaaagac gacaacctta actgggtgct tcaggcagag cagctgggta
    16021 cgggtcatgc aatgcagcag gccgcacctt tctttgccga tgatgaagac attttaatgc
    16081 tctacggcga cgtgccgctg atctctgtcg aaacactcca gcgcctgcgt gatgctaaac
    16141 cccagggtgg cattggtctg ctgacggtga aactggatga tccgaccggt tatggacgta
    16201 tcacccgtga aaatggcaaa gttaccggca ttgttgagca caaagatgcc accgacgagc
    16261 agcgtcagat tcaggagatc aacaccggca ttctgattgc caacggcgca gatatgaaac
    16321 gctggctggc gaagctgacc aacaataatg ctcagggcga atactacatc accgacatta
    16381 ttgcgctggc gtatcaggaa gggcgtgaaa tcgtcgccgt tcatccgcaa cgtttaagcg
    16441 aagtagaagg tgtgaataac cgcctgcaac tctcccgtct ggagcgcgtt taccagtccg
    16501 aacaggctga aaaactgctg ttagcaggcg ttatgctgcg cgatccggcg cgttttgatc
    16561 tgcgcggtac gcttactcac gggcgcgatg ttgaaattga tactaacgtt atcatcgagg
    16621 gcaacgtgac tctcggtcat cgcgtgaaaa tcggcaccgg ttgcgtgatt aaaaacagcg
    16681 tgattggcga tgattgcgaa atcagtccgt ataccgttgt ggaagacgcg aatctggcag
    16741 cggcctgtac tattggcccg tttgcccgtc tgcgtcctgg tgctgagttg ctggaaggtg
    16801 cacacgttgg taacttcgtt gagatgaaaa aagcgcgtct gggtaaaggc acgaaagccg
    16861 ggcatctgac ttacctgggc gatgcggaaa ttggcgataa cgttaacatc ggcgcgggaa
    16921 ccattacctg caactacgat ggtgcgaata aatttaagac cattatcggc gacgatgtgt
    16981 ttgtcggttc cgacactcag ctggtggccc cggtaacagt aggcaaaggc gcgaccattg
    17041 ctgcgggtac aactgtgacg cgtaatgtcg gcgaaaatgc attagctatc agccgtgtgc
    17101 cgcagactca gaaagaaggc tggcgtcgtc cggtaaagaa aaagtaattc tggccgggta
    17161 atccggtcat ctgggatgag gagataacat aatctccctc ccacaagcag taactataaa
    17221 aataacccca ctctctacaa ggctcggggc gcccgaaaaa acgggcatac aggttgaccg
    17281 acaacgatat aaatcggaat caaaaactat gtgtggaatt gttggcgcga tcgcgcaacg
    17341 tgatgtagca gaaatccttc ttgaaggttt acgtcgtctg gaataccgcg gatatgactc
    17401 tgccggtctg gccgttgttg atgcggaagg tcatatgact cgcctgcgtc gcctcggtaa
    17461 agtccagatg ctggctcagg cagcggaaga acatcctctg catggcggca ccggtattgc
    17521 tcacactcgc tgggcgaccc acggtgaacc ttcagaagct aacgcgcatc cgcatgtttc
    17581 tgaacacatt gtggtggtgc ataatggcat catcgaaaac catgaaccgc tgcgtgaaga
    17641 gttaaaagcg cgtggctata ccttcgtttc tgaaaccgac accgaagtga ttgcccatct
    17701 ggtgaactgg gagctgaaac aaggcgggac tctgcgtgag gctgttctgc gtgctatccc
    17761 gcagctgcgc ggtgcgtacg gtacagtgat catggactcc cgtcacccgg ataccctgct
    17821 agcggcacgt tctggtagtc cgctggtgat tggcctgggt atgggcgaaa actttatcgc
    17881 ttctgaccag ctggcgctgt tgccggtgac ccgtcgcttt atcttccttg aagagggcga
    17941 tattgcggaa atcactcgcc gttcggtaaa catcttcgat aaaactggcg cggaagtaaa
    18001 acgtcaggat atcgaatcca atctgcaata tgacgcgggc gataaaggta tttaccgtca
    18061 ctacatgcag aaagagatct acgaacagcc gaacgcgata aaaaacaccc ttaccgggcg
    18121 catcagccac ggtcaggttg atttaagcga gctgggaccg aacgccgacg aactgctgtc
    18181 gaaggttgag catattcaga tcctcgcctg tggtacttct tataactccg gtatggtttc
    18241 ccgctactgg tttgaatcgc tggcaggtat tccgtgcgac gtcgaaatcg cctctgaatt
    18301 ccgctatcgt aaatctgccg tgcgtcgtaa cagcctgatg atcaccttgt cacagtctgg
    18361 cgaaaccgcg gataccctgg cgggcctgcg tctgtcgaaa gagctgggtt accttggttc
    18421 gctggcaatc tgtaacgttc cgggttcttc tctggtgcgc gaatccgatc tggcgctgat
    18481 gaccaacgcg ggtacggaaa tcggcgtggc gtccactaaa gcattcacca ctcagttaac
    18541 tgtgctgttg atgctggtgg cgaagctgtc tcgcctgaaa ggtctggatg cctccattga
    18601 acatgacatt gtgcatggtc tgcaggcgct gccgagccgt attgagcaga tgctgtctca
    18661 ggataaacgc attgaagctc tggcagaaga tttctctgac aaacatcacg cgctgttcct
    18721 gggccgtggc gatcagtacc caatcgcgct ggaaggcgca ttgaagctga aagagatctc
    18781 ttacattcac gctgaagcct acgctgcagg cgagctgaaa cacggcccgc tggcgctgat
    18841 tgacgcggat atgccggtta tcgtcgttgc accgaacaac gaactgttgg aaaaactgaa
    18901 atccaacatt gaagaagttc gcgcgcgtgg cggtcaattg tatgtcttcg ccgatcagga
    18961 tgcgggtttt gtaagtagcg ataacatgca catcatcgag atgccgcatg tggaagaggt
    19021 gattgcaccg atcttctaca ccgttccgct gcagctgctg gcttaccatg tcgcgctgat
    19081 caaaggcacc gacgttgacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaatc
    19141 actatcatag ataaaaaagg gctgctgtgg gcggacaata aagtcttaaa ctgaacaaaa
    19201 tagatctaaa ctatgacaat aaagtcttaa actagacaga atagttgtaa actgaaatca
    19261 gtccagttat gctgtgaaaa agcatactgg acttttgtta tggctaaagc aaactcttca
    19321 ttttctgaag tgcaaattgc ccgtcgtatt aaagaggggc gtggccaagg gcatggtaaa
    19381 gactatattc catggctaac agtacaagaa gttccttctt caggtcgttc ccaccgtatt
    19441 tattctcata agacgggacg agtccatcat ttgctatctg acttagagct tgctgttttt
    19501 ctcagtcttg agtgggagag cagcgtgcta gatatacgcg agcagttccc cttattacct
    19561 agtgatacca ggcagattgc aatagatagt ggtattaagc atcctgttat tcgtggtgta
    19621 gatcaggtta tgtctactga ttttttagtg gactgcaaag atggtccttt tgagcagttt
    19681 gctattcaag tcaaacctgc agcagcctta caagacgagc gtaccttaga aaaactagaa
    19741 ctagagcgtc gctattggca gcaaaagcaa attccttggt tcatttttac tgataaagaa
    19801 ataaatcccg tagtaaaaga aaatattgaa tggctttatt cagtgaaaac agaagaagtt
    19861 tctgcggagc ttttagcaca actatcccca ttggcccata tcctgcaaga aaaaggagat
    19921 gaaaacatta tcaatgtctg taagcaggtt gatattgctt atgatttgga gttaggcaaa
    19981 acattgagtg agatacgagc cttaaccgca aatggtttta ttaagttcaa tatttataag
    20041 tctttcaggg caaataagtg tgcagatctc tgtattagcc aagtagtgaa tatggaggag
    20101 ttgcgctatg tggcaaatta atgaggttgt gctatttgat aatgatccgt atcgcatttt
    20161 ggctatagag gatggccaag ttgtctggat gcaaataagc gctgataaag gagttccaca
    20221 agctagggct gagttgttgc taatgcagta tttagatgaa ggccgcttag ttagaactga
    20281 tgacccttat gtacatcttg atttagaaga gccgtctgta gattctgtca gcttccagaa
    20341 gcgcgaggag gattatcgaa aaattcttcc tattattaat agtaaggatc gtttcgaccc
    20401 taaagtcaga agcgaactcg ttgagcatgt ggtccaagaa cataaggtta ctaaggctac
    20461 agtttataag ttgttacgcc gttactggca gcgtggtcaa acgcctaatg cattaattcc
    20521 tgactacaaa aacagcggtg caccagggga aagacgttca gcgacaggaa cagcaaagat
    20581 tggccgagcc agagaatatg gtaagggtga aggaaccaag gtaacgcccg agattgaacg
    20641 cctttttagg ttgaccatag aaaagcacct gttaaatcaa aaaggtacaa agaccaccgt
    20701 tgcctataga cgatttgtgg acttgtttgc tcagtatttt cctcgcattc cccaagagga
    20761 ttacccaaca ctacgtcagt ttcgttattt ttatgatcga gaatacccta aagctcagcg
    20821 cttaaagtct agagttaaag caggggtata taaaaaagac gtacgaccct taagtagtac
    20881 agccacttct caggcgttag gccctgggag tcgttatgag attgatgcca cgattgctga
    20941 tatttattta gtggatcatc atgatcgcca aaaaatcata ggaagaccaa cgctttacat
    21001 tgtgattgat gtgtttagtc ggatgatcac gggcttttat atcggctttg aaaatccgtc
    21061 ttatgtggtg gcgatgcagg cttttgtaaa tgcttgctct gacaaaacgg ccatttgtgc
    21121 ccagcatgat attgagatta gtagctcaga ctggccgtgt gtaggtttgc cagatgtgtt
    21181 gctagcggac cgtggcgaat taatgagtca tcaggtcgaa gccttagttt ctagttttaa
    21241 tgtgcgagtg gaaagtgctc cacctagacg tggcgatgct aaaggcatag tggaaagcac
    21301 ttttagaaca ctacaagccg agtttaagtc ctttgcacct ggcattgtag agggcagtcg
    21361 gatcaaaagc catggtgaaa cagactatag gttagatgca tctctgtcgg tatttgagtt
    21421 cacacaaatt attttgcgta cgatcttatt cagaaataac catctggtga tggataaata
    21481 cgatcgagat gctgattttc ctacagattt accgtctatt cctgtccagc tatggcaatg
    21541 gggtatgcag catcgtacag gtagtttaag ggctgtggag caagagcagt tgcgagtagc
    21601 gttactgcct cgccgaaagg tctctatttc ttcatttggc gttaatttgt ggggtttgta
    21661 ttactcgggg tcagagattc tgcgtgaggg ttggttgcag cggagcactg atatagctag
    21721 acctcaacat ttagaagcgg cttatgaccc agtgctggtt gatacgattt atttgtttcc
    21781 gcaagttggc agccgtgtat tttggcgctg taatctgacg gaacgtagtc ggcagtttaa
    21841 aggtctctca ttttgggagg tttgggatat acaagcacaa gaaaaacaca ataaagccaa
    21901 tgcgaagcag gatgagttaa ctaaacgcag ggagcttgag gcgtttattc agcaaaccat
    21961 tcagaaagcg aataagttaa cgcccagtac tactgagccc aaatcaacac gcattaagca
    22021 gattaaaact aataaaaaag aagccgtgac ctcggagcgt aaaaaacgtg cggagcattt
    22081 gaagccaagc tcttcaggtg atgaggctaa agttattcct ttcaacgcag tggaagcgga
    22141 tgatcaagaa gattacagcc tacccacata cgtgcctgaa ttatttcagg atccaccaga
    22201 aaaggatgag tcatgagtgc tacccggatt caagcagttt atcgtgatac gggggtagag
    22261 gcttatcgtg ataatccttt tatcgaggcc ttaccaccat tacaagagtc agtgaatagt
    22321 gctgcatcac tgaaatcctc tttacagctt acttcctctg acttgcaaaa gtcccgtgtt
    22381 atcagagctc ataccatttg tcgtattcca gatgactatt ttcagccatt aggtacgcat
    22441 ttgctactaa gtgagcgtat ttcggtcatg attcgaggtg gctacgtagg cagaaatcct
    22501 aaaacaggag atttacaaaa gcatttacaa aatggttatg agcgtgttca aacgggagag
    22561 ttggagacat ttcgctttga ggaggcacga tctacggcac aaagcttatt gttaattggt
    22621 tgttctggta gtgggaagac gacctctctt catcgtattc tagccacgta tcctcaggtg
    22681 atttaccatc gtgaactcaa tgtagagcag gtggtgtatt tgaaaataga ctgctcgcat
    22741 aatggttcgc taaaagaaat ctgcttgaat tttttcagag cgttggatcg agccttgggc
    22801 tcgaactatg agcgtcgtta tggcttaaaa cgtcatggta tagaaaccat gttggctttg
    22861 atgtcgcaaa tagccaatgc acatgcttta gggttgttgg ttattgatga aattcagcat
    22921 ttaagccgct ctcgttcggg tggatctcaa gagatgctga acttttttgt gacgatggtg
    22981 aatattattg gcgtaccagt gatgttgatt ggtaccccta aagcacgaga gatttttgag
    23041 gctgatttgc ggtctgcacg tagaggggca gggtttggag ctatattctg ggatcctata
    23101 caacaaacgc aacgtggaaa gcccaatcaa gagtggatcg cttttacgga taatctttgg
    23161 caattacagc ttttacaacg caaagatgcg ctgttatcgg atgaggtccg tgatgtgtgg
    23221 tatgagctaa gccaaggagt gatggacatt gtagtaaaac tttttgtact cgctcagctc
    23281 cgtgcgctag ctttaggcaa tgagcgtatt accgctggtt tattgcggca agtgtatcaa
    23341 gatgagttaa agcctgtgca ccccatgcta gaggcattac gctcgggtat cccagaacgc
    23401 attgctcgtt attctgatct agtcgttccc gagattgata aacggttaat ccaacttcag
    23461 ctagatatcg cagcgataca agaacaaaca ccagaagaaa aagcccttca agagttagat
    23521 accgaagatc agcgtcattt atatctgatg ctgaaagagg attacgattc aagcctgtta
    23581 attcccacta ttaaaaaagc gtttagccag aatccaacga tgacaagaca aaagttactg
    23641 cctcttgttt tgcagtggtt gatggaaggc gaaacggtag tgtcagaact agaaaagccc
    23701 tccaagagta aaaaggtttc ggctataaag gtagtcaagc ccagcgactg ggatagcttg
    23761 cctgatacgg atttacgtta tatctattca caacgccaac ctgaaaaaac catgcatgaa
    23821 cggttaaaag ggaaaggggt aatagtggat atggcgagct tatttaaaca agcaggttag
    23881 ccatgagaaa ctttcctgtt ccgtactcga atgagctgat ttatagcact attgcacggg
    23941 caggcgttta tcaagggatt gttagtccta agcagctgtt ggatgaggtg tatggcaacc
    24001 gcaaggtggt cgctacctta ggtctgccct cgcatttagg tgtgatagca agacatctac
    24061 atcaaacagg acgttacgct gttcagcagc ttatttatga gcatacctta ttccctttat
    24121 atgctccgtt tgtaggcaag gagcgccgag acgaagctat tcggttaatg gagtaccaag
    24181 cgcaaggtgc ggtgcattta atgctaggag tcgctgcttc tagagttaag agcgataacc
    24241 gctttagata ctgccctgat tgcgttgctc ttcagctaaa taggtatggg gaagcctttt
    24301 ggcaacgaga ttggtatttg cccgctttgc catattgtcc aaaacacggt gctttagtct
    24361 tctttgatag agctgtagat gatcaccgac atcaattttg ggctttgggt catactgagc
    24421 tgctttcaga ctaccccaaa gactccctat ctcaattaac agcactagct gcttatatag
    24481 cccctctgtt agatgctcca cgagcgcaag agctttcccc aagccttgag cagtggacgc
    24541 tgttttatca gcgcttagcg caggatctag ggctaaccaa aagcaagcac attcgtcatg
    24601 acttggtggc ggagagagtg aggcagactt ttagtgatga ggcactagag aaactggatt
    24661 taaagttggc agagaacaag gacacgtgtt ggctgaaaag tatattccgt aagcatagaa
    24721 aagcctttag ttatttacag catagtattg tgtggcaagc cttattgcca aaactaacgg
    24781 ttatagaagc gctacagcag gcaagtgctc ttactgagca ctctataacg acaagacctg
    24841 ttagccagtc tgtgcaacct aactctgaag atttatctgt taagcataaa gactggcagc
    24901 aactagtgca taaataccaa ggaattaagg cggcaagaca gtctttagag ggtggggtgc
    24961 tatacgcttg gctttaccga catgacaggg attggctagt tcactggaat caacagcatc
    25021 aacaagagcg tctggcaccc gcccctagag ttgattggaa ccaaagagat cgaattgctg
    25081 tacgacaact attaagaatc ataaagcgtc tagatagtag ccttgatcac ccaagagcga
    25141 catcgagctg gctgttaaag caaactccta acggaacctc tcttgcaaaa aatctacaga
    25201 aactgccttt ggtagcgctt tgcttaaagc gttactcaga gagtgtggaa gattatcaaa
    25261 ttagacggat tagccaagct tttattaagc ttaaacagga agatgttgag cttaggcgct
    25321 ggcgattatt aagaagtgca acgttatcta aagagcggat aactgaggaa gcacaaagat
    25381 tcttggaaat ggtttatggg gaagagtgag tggttaggct agctacattt aatgacaatg
    25441 tgcaggttgt acatattggt catttattcc gtaactcggg tcataaggag tggcgtattt
    25501 ttgtttggtt taatccaatg caagaacgga aatggactcg atttactcat ttgcctttat
    25561 taagtcgagc taaggtggtt aacagtacaa caaagcaaat aaataaggcg gatcgtgtga
    25621 ttgagtttga agcatcggat cttcaacgag ccaaaataat cgattttcct aatctctcgt
    25681 cctttgcttc cgtacgcaac aaggatggag cgcagagttc atttatttac gaagctgaaa
    25741 caccatatag caagactcgt tatcacatcc cacagttaga gctagctcgg tcattatttt
    25801 taattaactc ctatttctgt cgaagctgtt tgagcagtac cgctttacag caagagttcg
    25861 acgttcagta tgaggttgag cgagatcatt tagagataag gatcttaccc agttcatcgt
    25921 ttcctaaagg ggcgttagag ctgtcggccg tagtgcagct tttggtttgg ttgttttcgg
    25981 atcaagatgt tatggattcg tatgaaagta tttttaggca ctatcaacaa aatagagaaa
    26041 ttaagaacgg cgttgaaagc tggtgcttta gctttgaccc tccgcccatg cagggttgga
    26101 aattacatgt aaaaggacgt tcttctaacg aggataagga ttatttagtt gaggaaatag
    26161 taggtttaga aatcaacgct atgcttccta gcacaacagc tattagccat gcctcttttc
    26221 aggaaaagga ggcaggtgat ggtagtacgc agcacatagc ggtttcaaca gagtcagttg
    26281 ttgatgatga gcatctacag ttggacgatg aggaaacagc caatatagac acagacacac
    26341 gagtcataga ggctgagccg acatggataa gttttagtag acctagtcga attgaaaaat
    26401 ctcgcagggc aagaaaaagt agccaaacta ttttagaaaa agaagaagca acaacaagtg
    26461 aaaatagtaa tttggttagt actgatgagc cacacttagg tggtgtccta gcagcggcag
    26521 atgtgggtgg gaagcaggat gcaaccaatt acaactctat ttttgctaat cgatttgctg
    26581 cttttgatga gctactttca attctaaaaa ctaaatttgc atgtcgggtg ctttttgaag
    26641 aaaccttggt tttgccaaaa gttgggcgta gccgattaca tctgtgtaaa gatggctcac
    26701 caagagtgat taaagccgtt ggggtgcaac gtaatggcag tgaatttgta ttgctagagg
    26761 tggatgcatc ggatggggtg aaaatgcttt ctaccaaagt gttgagtggc gttgatagcg
    26821 aaacatggcg gaatgatttt gaaaagatac ggcgtggagt ggtgaagagc tcattgaatt
    26881 ggccaaatag tttgtttgat caattatatg gacaagacgg gcatagaggg gtgaatcatc
    26941 caaaggggtt gggggagctg caagtatcga gagaggatat ggaagggtgg gctgagagag
    27001 tggttagaga gcaatttacg cattaaagga atgactgaaa gagcctgtaa acccttttgt
    27061 gtaagtgctt ttgccggtca gttaaaggtg gccatttaaa cggtcaccaa attcgatcat
    27121 aaaacggttc atggccggct tccagttgcg gatcggcatc gtccatttct tggtcgccgc
    27181 ctggatagcc aggtacacca ccttcatcgc tgattcgtcc gtagggaaca ccttgagttc
    27241 atattaatgg aattttctac aaatagcctc cgtggttttg aggggggatt acagacgatc
    27301 catagtagta atccaatgag ttcttgagcg cggcgacatg tttggacgcc ttggcaaaaa
    27361 ttagagcctg cttgaagtgc aggcgagcac gtgcttggac gattgatcca ttcgcggtca
    27421 aaaactcaat cttggatgac agcgtgtcag gaaatccaat atcgaagtcc cgccccgtgt
    27481 caaagtaggg catttcatga tcaaaggacg aagcttccca ggccacttcc ctaatattcc
    27541 agccgctaga aacaagattt cgtacgactt catcaacttt tttgctttcc aacttgtcac
    27601 aaagtgcata ggcaagagct ttggcggcag acacgtgcgt ttcctcttaa acataggtcg
    27661 aacggatacc cgaggcaaag cggagcatta gtcccaaagc atctccggta gatccggagg
    27721 ctccatactg aaggcatggc atcttgggca ttgatatttg aaacttctct ctagatagca
    27781 gggatttctt ggccttgcct catgctgata gcttgtaaat atcacacgat ccttagcccc
    27841 acaaagtgag catactccgt agctttcatt tatagaaatc gtcgttagat tgtcgcagca
    27901 tgtgcacgtt ccgactattt gctcaggaag catcgccata ccttggccta ggtaaagaag
    27961 atcagactcg tagccacatc ggtggcaact gagcttgtac gcaatgccca tctggttgtc
    28021 tcacctgaat cgtaacgtca gagctattca tccatccatt cggcatcaat gtatgccact
    28081 aagccgacca gctgaccgtt tggactgaag tatgttgcct gcaccccact tgacatctca
    28141 tcaataccac cgcactgcgc taggcgccgg tcccacttgc ccacagaagc gtcaggatcg
    28201 gctgagttcc ccaagaccct tgtgagccat gcctccttta actcaacctc tcgaatgatt
    28261 gcgtcggcca gctcctccgc gttatctagt tcgacatagt ctggaattcg gacgtacgtt
    28321 ctgaacacag ctggatactt acttgggcga ttgtcataca tgacatcaac aatgtacccg
    28381 tttgtgtaac cgtctcttgg aggttcgtat gacactagtg gttcccctca gcttgcgact
    28441 agatgttgag gcctaacatt ttattagaga gcaggctagt tgcttagata catgatcttc
    28501 aggccgttat ctgtcagggc aagcgaaaat tggccattta tgacgaccaa tgccccgcag
    28561 aagctcccat ctttgccgcc atagacgccg cgcccccctt ttggggtgta gaacatcctt
    28621 ttgccagatg tggaaaagaa gttcgttgtc ccattgttgg caatgacgta gtagccggcg
    28681 aaagtgcgag acccatttgc gctatatata agcctacgat ttccgttgcg actattgtcg
    28741 taattggatg aactattatc gtagttgctc tcagagttgt cgtaatttga tggactattg
    28801 tcgtaattgc ttatggagtt gtcgtagttg cttggagaaa tgtcgtagtt ggatggggag
    28861 tagtcatagg gaagacgagc ttcatccact aaaacaattg gcaggtcagc aagtgcctgc
    28921 cccgatgcca tcgcaagtac gaggcttaga accaccttca acagatcgcg catagtcttc
    28981 cccagctctc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct tgaacgaatt
    29041 gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg aacaaattct
    29101 tccaactgat ctgcgcgcga ggccaagcga tcttcttgtc caagataagc ctgcctagct
    29161 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
    29221 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
    29281 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
    29341 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
    29401 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
    29461 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
    29521 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
    29581 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
    29641 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
    29701 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
    29761 ggttcgagat ggcgctcgat gacgccaact acctctgata gttga
//
LOCUS       DAJTUP010000040        29255 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTUP010000040 DAJTUP010000000
VERSION     DAJTUP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29255
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.040"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..450
                     /locus_tag="OO468_001870"
     CDS             <1..450
                     /locus_tag="OO468_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6259007.1"
                     /translation="ERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADD
                     ITMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMV
                     HHHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            472..1881
                     /locus_tag="OO468_001871"
     CDS             472..1881
                     /locus_tag="OO468_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259008.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1859..2638
                     /locus_tag="OO468_001872"
     CDS             1859..2638
                     /locus_tag="OO468_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR6259009.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2635..3495
                     /locus_tag="OO468_001873"
     CDS             2635..3495
                     /locus_tag="OO468_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR6259010.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3643..4218)
                     /locus_tag="OO468_001874"
                     /pseudo
     CDS             complement(3643..4218)
                     /locus_tag="OO468_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4235..4495)
                     /locus_tag="OO468_001875"
     CDS             complement(4235..4495)
                     /locus_tag="OO468_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6259011.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4486..5757)
                     /locus_tag="OO468_001876"
     CDS             complement(4486..5757)
                     /locus_tag="OO468_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6259012.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5835..6197)
                     /gene="queD"
                     /locus_tag="OO468_001877"
     CDS             complement(5835..6197)
                     /gene="queD"
                     /locus_tag="OO468_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR6259013.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6516..8315
                     /gene="cysJ"
                     /locus_tag="OO468_001878"
     CDS             6516..8315
                     /gene="cysJ"
                     /locus_tag="OO468_001878"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR6259014.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8315..10027
                     /gene="cysI"
                     /locus_tag="OO468_001879"
     CDS             8315..10027
                     /gene="cysI"
                     /locus_tag="OO468_001879"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR6259015.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10101..10835
                     /gene="cysH"
                     /locus_tag="OO468_001880"
     CDS             10101..10835
                     /gene="cysH"
                     /locus_tag="OO468_001880"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR6259016.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11099..11251
                     /locus_tag="OO468_001881"
     CDS             11099..11251
                     /locus_tag="OO468_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6259017.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11445..12536
                     /locus_tag="OO468_001882"
                     /pseudo
     CDS             11445..12536
                     /locus_tag="OO468_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12682..13719)
                     /gene="iap"
                     /locus_tag="OO468_001883"
     CDS             complement(12682..13719)
                     /gene="iap"
                     /locus_tag="OO468_001883"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR6259018.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            13971..14879
                     /gene="cysD"
                     /locus_tag="OO468_001884"
     CDS             13971..14879
                     /gene="cysD"
                     /locus_tag="OO468_001884"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR6259019.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14881..16308
                     /gene="cysN"
                     /locus_tag="OO468_001885"
     CDS             14881..16308
                     /gene="cysN"
                     /locus_tag="OO468_001885"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR6259020.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16308..16913
                     /gene="cysC"
                     /locus_tag="OO468_001886"
     CDS             16308..16913
                     /gene="cysC"
                     /locus_tag="OO468_001886"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR6259021.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            16963..17286
                     /locus_tag="OO468_001887"
     CDS             16963..17286
                     /locus_tag="OO468_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR6259022.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17480..17791
                     /gene="ftsB"
                     /locus_tag="OO468_001888"
     CDS             17480..17791
                     /gene="ftsB"
                     /locus_tag="OO468_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR6259023.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17810..18520
                     /gene="ispD"
                     /locus_tag="OO468_001889"
     CDS             17810..18520
                     /gene="ispD"
                     /locus_tag="OO468_001889"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR6259024.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18520..18999
                     /gene="ispF"
                     /locus_tag="OO468_001890"
     CDS             18520..18999
                     /gene="ispF"
                     /locus_tag="OO468_001890"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR6259025.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            18996..20045
                     /gene="truD"
                     /locus_tag="OO468_001891"
     CDS             18996..20045
                     /gene="truD"
                     /locus_tag="OO468_001891"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR6259026.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20026..20787
                     /gene="surE"
                     /locus_tag="OO468_001892"
     CDS             20026..20787
                     /gene="surE"
                     /locus_tag="OO468_001892"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR6259027.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20781..21407
                     /gene="pcm"
                     /locus_tag="OO468_001893"
     CDS             20781..21407
                     /gene="pcm"
                     /locus_tag="OO468_001893"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR6259028.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21547..22686
                     /gene="nlpD"
                     /locus_tag="OO468_001894"
     CDS             21547..22686
                     /gene="nlpD"
                     /locus_tag="OO468_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR6259029.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22749..23741
                     /gene="rpoS"
                     /locus_tag="OO468_001895"
     CDS             22749..23741
                     /gene="rpoS"
                     /locus_tag="OO468_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR6259030.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23861..24268)
                     /locus_tag="OO468_001896"
     CDS             complement(23861..24268)
                     /locus_tag="OO468_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR6259031.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24415..25008
                     /locus_tag="OO468_001897"
     CDS             24415..25008
                     /locus_tag="OO468_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR6259032.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25008..25955
                     /locus_tag="OO468_001898"
                     /pseudo
     CDS             25008..25955
                     /locus_tag="OO468_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26228..26791)
                     /locus_tag="OO468_001899"
     CDS             complement(26228..26791)
                     /locus_tag="OO468_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259033.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            26956..27207
                     /locus_tag="OO468_001900"
     CDS             26956..27207
                     /locus_tag="OO468_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6259034.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27209..>29255
                     /locus_tag="OO468_001901"
     CDS             27209..>29255
                     /locus_tag="OO468_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6259035.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     "
BASE COUNT         7130 a         7260 c         7928 g         6937 t
ORIGIN      
        1 gaacgtgtgc agatcctcaa aaccggcaac atgggcttta ccaccgaagt gtccggctgc
       61 tggagctgca tccacgaaat ctacgaaagc gttattaacc gtattcgtac tgagttcccg
      121 cacgccgacg acatcaccat gctgggcggt cactcctctc atagctatca gaacggcacc
      181 aacatgtact tcgtctatga ctacaacgtt gttgactgta agccggaaga ggaaatcgac
      241 aagtaccaca atccgctcaa caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg
      301 atggtgcacc accatggcat tggtaaacat cgcgttcact ggagcaagct ggaacacggc
      361 agcgcgtggg cgttgctgga agggctgaaa aagcagttcg atcctaacgg cattatgaat
      421 acgggtacta tctatccgat tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg
      481 cgttgccttt tcggcttctc aggcgagaag ccgttcttat taccggacaa tgaaggggta
      541 aagatgaaca cttcaccggt gcgaatggat gatttaccgc ttaaccgttt tcactgtcga
      601 attgctacgc tcactttcgg cgcacacctg accgacggtt atgttctagg cgtcattggt
      661 tacgccatta ttcaacttac gcccgccatg caactgacgc cgtttatggc gggaatgatc
      721 ggcggctcgg cgctccttgg tttgtttctt ggcagcctgg ttcttgggtg gatctccgac
      781 catattggtc ggcaaaaaat cttcaccttc agctttttgc tgattacgct cgcttcgttc
      841 ttgcaatttt ttgccaccac gccagagcat cttattgggc tgcgcatttt gatcggcatt
      901 ggtctgggag gcgattactc agtaggtcac accttgctgg ctgaattttc cccgcgccgc
      961 catcgcggta ttttgctggg cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca
     1021 agtattgccg gacatcactt tatttccgaa aacccggagg cctggcgctg gttgctggca
     1081 tcggcagctc tgcccgcgtt gttgattacg ttattacgct ggggaacgcc agaatcgcca
     1141 cgttggctac tgtgccaggg gcgttttgca gaagctcacg ctatcgtgca tcgctatttt
     1201 ggtccccatg ttttactggg cgatgaagtg gtaacggcga cccataaaca catcaaaacc
     1261 ttgttctctt cgcgttactg gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc
     1321 gtaatcccat ggtttgtgat ttatacctgg ctgccaacta tcgcccagac tattggtctg
     1381 gaagatgcgc tgactgccag cctgatgctt aatgcgttgt taattgtggg cgcgctgctg
     1441 ggattagttc tgacgcacct gctggcacat cgcaaatttt tgctgggaag ttttttgctg
     1501 ctggcggcaa cgctggtagt aatggcctgt ttgccttccg gcagttcatt aacgctgctg
     1561 ctttttgttc tcttcagcac caccatttcg gcagtcagta atctggtggg cattttgcct
     1621 gcggaaagtt ttcctactga cattcgctcg ctgggagtcg gttttgccac cgccatgagt
     1681 cgacttggcg cggcggtaag tactggcctg ctgccgtggg tgctggcgca gtggggaatg
     1741 caagtcacct tattgctcct agcgacagtg ttgttggttg gttttgttgt gacctggcta
     1801 tgggcaccag aaactaaagc cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat
     1861 gaacattctg ttagcgttta aagccgaacc ggatgccgga atgctggcgg aaaaagagtg
     1921 gcaggcggcg gctcagggta atagcggacc ggatgtttcg ctactgcgaa gtttactcgg
     1981 tgctgatgaa caggccgctg ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat
     2041 gtctttaacc gcgttgagca tgggggatga acgggcgttg cactggctgc gctatctcat
     2101 ggctcttggg tttgaggaag ctgtattgct ggagacggcg gcagatctgc gctttgcgcc
     2161 ggaatttgtc gctcgccata ttgccgaatg gcagcgtcag aatccgctgg atctgataat
     2221 taccggcagc caaagcagcg aagggcagaa tgggcaaacg ccatttttgc tggcggaaat
     2281 gctgggctgg ccctgcttca cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac
     2341 cctcgaacag cgtactgaac atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat
     2401 tgccgtgcgt cagtgcggtg aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc
     2461 tgcggggaaa gcagaaatta tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg
     2521 tctgcaactg gcaagagcag agcaacgacg cggagcgacg ctgattgatg ggcaaacggt
     2581 agcagaaaaa gctcaaaagc tatggcagga ctatttacgc caaaggatgc agccatgaat
     2641 atcgcaattg tgacaataaa tcaggagaat gccgcaatag cgagctggct ggcggcgcag
     2701 gatttcagcg gttgtacgct ggcgcactgg caaatagaac ctcagcccgt agtggcggaa
     2761 caggtgctgg atgcgatggt ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt
     2821 ccgccgggaa cgtttggcga tgaactgtcg acccggctgg cctggcgttt acatggtgca
     2881 agcatctgtc aggtgacttc gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg
     2941 ggaaatgcgt taacggcgac gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg
     3001 cgtcaggcgg gagcagcgaa aaacgccacg ttgccctctg gaatgcagca actgaacatt
     3061 gtcccaggtg cgcttcccga ctggctggtt agcacagagg atctgaaaaa tgttagccgc
     3121 gacccattag ctgaggccag acgggtgctg gtggtgggac agggcggaga ggcagataac
     3181 caggaaatcg ttatgctggc agaaaagctg ggagccgaag tgggctacag tcgggcgcga
     3241 gtgatgaacg gtggcgttga tgctgaaaaa gtgattggta tttccggaca tctgctggca
     3301 cctgaggtgt gcattgttgt tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc
     3361 aatagcaagt ttatggtggc gattaatcat gacgccagcg cagcggtatt ttcacaggca
     3421 gatattggcg tggttgatga ctggaaagtg gtgctggagg cactggtgac aaacattcac
     3481 gcagaatacc aataacgtgc tgccggatga tcctgcctac ggttctggac tggggacctg
     3541 taggcctgat aagacgcgtt aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg
     3601 gccaacaaat caaaacaaat tcaattggtt acgattatcc cgtcaaagca attttttcgc
     3661 taacgtccag actccggtat tcgtggtgga aagcgccacg acacccgcgt taatcgcatt
     3721 acgcgcatct tcttcatcgc ataccagccc gccagcaatc agcggttggc ggattttttc
     3781 cgtcacccag cccagcactt tgggcatgca gcctggcagg atctcaatac aatccggatt
     3841 cgattgcgca acttacttat caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg
     3901 atgaatacag aaaaaaactt ccgctcttgc cgcttttagc atcgatgctt tggtgctgat
     3961 aattccatcc gcctcggtca ccagtttcaa aaactgaatc accacttctt tatttgacgc
     4021 gccttccagc aaatcaacat gaataaaagc atatttcccg gcgttcttaa tctttttaac
     4081 gatattactg atggtgcaga tattgccgta caacacggaa ataaactggc attcagaatc
     4141 gatggccagt tgcaggctgg cattatcttt aacggcagca atcaccggat tctggcggag
     4201 caggtgtaag aggggcataa attatcctta ttatttatcc aaagcggtag gtgatgccga
     4261 atccggaagg cggatagcgc cactgttgta gtgtggattc gtcgcacagc aaacggcagg
     4321 tgccacactc caggcagccg cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa
     4381 aaagacctac cggacaggcg ttaatcaacc gttgtaccat ctgacgctca acggagtcag
     4441 ccggaacaat gtgcggcgca tcagcaacgc gccagagatt acgggctaca gacatcgcag
     4501 actcctgata acatcgcccg ccagatgcca caggccatga cgacgtaaat gatgccagaa
     4561 taactggcgc agcggtggaa caggtttatc accctgatcc cataaatcgc gggaaatatc
     4621 ctgcattaac gcaggccacg cccgatacct gccagggcgt tgcaaaagcg ccggaacatg
     4681 ctgataacgt tgtagaacat cccatagcag gctgcgctct acgttgtgat gataaagcgc
     4741 aaacagattt tgcggctcgc ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg
     4801 tgcgccagtc agcgccatat ccatgccgcg cacggaaatt ccggtattga cgcaactgcg
     4861 caacgcatcg cccaccagca gccagccgtt accggcgtat tgcaccggca tactgtgcaa
     4921 gccaccttct ggcaccagat gcgcaccata ctccagtgat tccgtgtttt tgataagcgt
     4981 gcgcactgcc ggatgcgctt taaagcgagt cagcagctcg cttgccggaa cacgactttg
     5041 cgtaagggaa gagagcgggc aaacaatccc taacgagagc gtttgttgat tagtataaag
     5101 aaatgcgccg ccgggtaagt catcacagat tccgccgctg aacagcaacg ctgcgccttc
     5161 gttattctcc agatgaaaac gttcttcaat agcggatgtt tccagcgaca gcacttcttt
     5221 gatccccaac gccatcgctt cgccagcagg acgagtcact aacccgtgac gttcagccag
     5281 gacgctgttg gcaccttctg ccagcaccac ataacgggcg cggagaatat cgtcaccaca
     5341 aataacgcca cagactctgc cgttttcttc atacagtgca tccaccgtcg ctccggggat
     5401 gcattcgaca ccttcttttt cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa
     5461 tacattccag gattcaccgc cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt
     5521 taacagggaa aggctttcgt gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg
     5581 cgggaggagt tccgcgagtg catgggtata taaccgcctg ccggaaagat ttttgctgcc
     5641 ggggatttca gcgcgttcga gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc
     5701 gcaagcggtg cctgcaatac cggcaccaat aataataatg tcgcagtcgt cttccatgtc
     5761 atgccccaga tatccagaac acaacaccct aacatagcgt tactaaaggg aaattgaccg
     5821 ccgacacact cttatcattc gccgcgatag atacaccctg cggtgcaggt ttcttttacc
     5881 atcaccgcac ttaacagcgg cacaacgggt ttaacctgat cccaaatcca ttttgctaaa
     5941 acttcgctgg ttggattttc cagacctgga atatcattga gataatggtg atcgaggcgc
     6001 tcataagttg gcttaaacgc cgctttcagt tcagcgaaat cgataatcca gcccgtatgc
     6061 ggatcgactt ccccggtaat ttccagtcgc accataaagg aatgcccgtg caggcgacca
     6121 catttatgcc cttccgggac atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta
     6181 aataacgtgg tggacatcat aatttctcta caggacgata aaaccgccgt agagtaccgg
     6241 aaaggctgtt ttttcgcatt atctaaaacg actttgttgc gcaaaatcgc tgatttatct
     6301 taatgattgg ctaaattcat ttgtttttca ttaggttggt taatctattt tgttgttaaa
     6361 gactattgct aaaacaggtt agtcgatttg gttattagtt atcgctatcc cgtctttaat
     6421 ccacaccgtt caccccgtta accttacctt ctcttctgtt ttatgggcgc tgacagggcg
     6481 cagaaacagc tttgcttact ggaacataac gacgcatgac gacacaggtc ccaccttccg
     6541 cgttgcttcc gttgaacccg gagcaactgg cacgccttca ggcggccacg accgatttaa
     6601 cgcctaccca gcttgcctgg gtttctggct atttctgggg cgtgctcaac cagcagcctg
     6661 ttgcgcttgc agtgacgcca gcgcccgccg cagaaatgcc gggtataacg attatctctg
     6721 cttcgcaaac cggcaatgcg cgccgggttg ctgaagcatt acgcgatgat ttattagcag
     6781 caaaactgaa cgttaagctg gtgaacgcgg gcgactataa attcaaacaa atcgccagcg
     6841 aaaaactgct catcgtagtg acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg
     6901 tcgcgctgca taagttcctg ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg
     6961 ccgtgtttag cctcggcgat agctcttatg aatttttctg ccagtccggg aaagattttg
     7021 acagcaagct ggcggaactg gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg
     7081 aataccaggc tgctgccagc gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg
     7141 cgcctgtcgc ggcaccttcg caatccgtcg ctactggcgc ggtaaatgaa atccacacca
     7201 gcccgtacag caaagacgcg ccgctggtgg ctagcctctc tgttaaccag aaaattactg
     7261 ggcgtaactc tgaaaaagac gttcgccata tcgaaattga cttaggcgac tcgggcctgc
     7321 gttaccagcc gggtgacgcg ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag
     7381 aacttgtcga actgctgtgg ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt
     7441 tgcctctgaa cgaagcgcta cagtggcact tcgaactgac cgtcaacacc gccaacattg
     7501 ttgagaatta cgccacgctt acccgcagtg aaacactgct gccgctggtg ggcgataaag
     7561 cgaagttaca gcattacgcc gcgacgacgc tgattgttga catggtgcgt ttctccccgg
     7621 cacagcttga tgccgaagcg ctaattaatc tgctgcgccc gctgaccccg cgtctctatt
     7681 ccattgcctc ctcgcaggcg gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc
     7741 gttacgacgt ggaaggccgc gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg
     7801 tggaagaaga gggcgaagtc cgcgtattta tcgaacataa cgataacttc cgcctgcccg
     7861 ctaacctgga aaccccggtg attatgattg gcccaggcac cggtattgcg ccgttccgcg
     7921 cctttatgca gcaacgcgcc gctgacgaag cgccaggtaa aaactggctg ttctttggca
     7981 atccgcattt tacggaagac ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg
     8041 gtgtgctgac gcggatcgat ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac
     8101 aagataaact gcgcgaacag ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca
     8161 tttatgtctg cggcgacgct aatcgcatgg cgaaagacgt tgagcaggca cttctggaag
     8221 tgattgccga atttggtggc atggacaccg aagcggcgga tgaattttta agtgagctgc
     8281 gcgtagagcg ccgttatcag cgagatgtct actaatgagc gaaaaacatc cagggccttt
     8341 agtggtcgaa ggaaaactga cagacgccga gcgcatgaag cttgaaagca actacctgcg
     8401 cggcaccatt gcggaagatt taaacgacgg tctgaccggc ggctttaagg gcgataactt
     8461 cctgctgatc cgcttccacg gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg
     8521 tgctgaacag aagctggagc cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg
     8581 agttatcacc accaaacagt ggcaggcgat cgataaattt gccggtgaaa acaccatcta
     8641 tggcagcatt cgcctgacca accgccagac gtttcagttc cacggcattc tgaaaaagaa
     8701 cgtcaaaccg gtgcaccaga tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa
     8761 cgacatgaac cgtaacgtac tctgcacctc gaacccttac gagtcgcagc tgcacgcaga
     8821 agcgtacgag tgggcgaaga agatttctga acatctgctg ccgcgtaccc gcgcgtatgc
     8881 ggagatctgg ctcgatcagg aaaaagtcgc caccaccgat gaagaaccga tccttggtca
     8941 gacttacctg ccgcgtaagt tcaaaaccac ggtagtgatc ccgccgcaga acgatattga
     9001 tctgcacgcc aacgacatga acttcgtggc gatcgcggaa aacggcaagc tggtgggctt
     9061 caacctgctg gtgggcggtg ggctttctat cgaacacggt aacaagaaaa cctacgcccg
     9121 cacggcgagt gagtttggct atctgccgct agagcatacg ctagcggtgg cggaagccgt
     9181 cgtgacaact cagcgtgact ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata
     9241 cacactggag cgcgtggggg ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat
     9301 caaatttgaa ccgatccgcc cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt
     9361 taagggcatt gatgataact ggcacctgac gctgtttatc gaaaatggtc gcatccttga
     9421 ttatccgggg cgtccgctga aaactggcct gctggagatc gcgaagatcc acaaaggtga
     9481 tttccgcatt acggcgaacc agaatctgat catcgccggt gtgccggaaa gcgagaaagc
     9541 gaagatcgag aaaattgcca aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga
     9601 aaactcgatg gcctgcgtgt cattcccgac ttgcccgctg gcgatggcgg aagcggagcg
     9661 tttcctgccg tcttttatcg acaacatcga taatttaatg gtgaaacatg gtgtcagcga
     9721 tgagcatatc gtgatgcgtg taacaggctg cccgaacggt tgtggtcgcg cgatgctggc
     9781 ggaagtgggc ctggtgggta aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg
     9841 cattgggaca cgtatcccac ggatgtataa agaaaacatc accgagccgg aaatcctggc
     9901 gtcgcttgat gaactgatag ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg
     9961 cgactttacg gtgcgtgcgg gcatcattcg cccggtgctc gatccggcgc gcgatttgtg
    10021 ggattaacca tcagcccggt cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca
    10081 aggcaaacag tgaggaatct atgtccaaac tcgatctaaa cgccctgaac gaactgccga
    10141 aggtagatcg cattctggcg ctggcagaaa ctaacgccga actggaaaaa ctggacgctg
    10201 aaggccgcgt tgcctgggcg ctggataatc tgcccggtga atatgtactt tcttccagct
    10261 ttggcattca ggcggcggtg agcctgcatc tggtgaatca gattcacccg gatattccgg
    10321 tgatcctcac cgacaccggc tatctgttcc cggaaaccta ccgctttatt gacgagttaa
    10381 cggacaaact caagctcaac ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg
    10441 aagcacgcta cggcaaactg tgggaacagg gcgttgaagg cattgaaaag tacaatgaca
    10501 tcaacaaagt cgaaccgatg aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg
    10561 ctggcctgcg gcgtgaacaa tccggcagcc gtgccaattt accggtgctg gcaattcagc
    10621 gtggcgtatt taaagtgctg ccgattatcg actgggataa ccgaactatt tatcagtacc
    10681 tgcaaaaaca tggcctgaaa tatcacccat tatgggacga aggatattta tcggtgggcg
    10741 atacccatac aacccgtaaa tgggaacccg gcatggcgga agaagaaacg cgtttctttg
    10801 gcttaaaaag ggaatgtgga ttacacgaag ggtaaatatt gccggataaa ttaaacgcct
    10861 ctgtcagaaa tgatgggggc gtttttattt tgcgagcagc attccggcat ttaaaggtga
    10921 acatcgcagg gaaaattaac aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt
    10981 taagcattcg cgaggttcca gatggacaaa agccccaggc gatatttcta tcaacctgag
    11041 gctcgcgttc gaacgtagca attcggatgt tagtctcttc tttgcaggga ggcaagacat
    11101 gctgacaaaa tatgcccttg tggcaatcat cgtactgtgt tgtacagtac tgggattcac
    11161 gttgatggta ggtgactcgt tatgtgagtt gagtatcaga gaacgtggta tggagtttaa
    11221 ggcagttctc gcttacgaat cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac
    11281 tgaattgtga tggtttggtc tcctgcaccc tttttttaaa tttatatttt ttatgtgaat
    11341 atatagtctc tatttattat tatgaatttc aaatgaaaat cattaattta ttcataatgt
    11401 aaatgtttta ttggctgata tatatcatag atagttttta aaatatgcgt aaatatcctt
    11461 taagtttact gaaggataaa aatattgtga ctttctttga tttctgggga aaaaaccgac
    11521 gtggcgagaa agagggtatc gacgactatc accttctttg ctggcatttg ctggatgtgg
    11581 ccgcaatggg ctatttaatg gttaaaagaa attgcttcgg gctggctgat tattttcgtc
    11641 aattagggat ttctgacaag gaacaggcag ctcgattttt cgcctggttg ctgtgctggc
    11701 acgatattgg aaaatttgcc cgctcttttc aacaacttta cctgccccct gaattcaaga
    11761 ttcaggaagg tgccagaaaa aattacgaaa aaatctctca ttcaacgctg ggctactggt
    11821 tgtggaatca ttatttaagt gaatgtcagg agttacttcc ttcatcttcg ctctctccgc
    11881 gcaaacttag gcgagtgata gaaatgtgga tgcccgtgac gacagggcat catggtcggc
    11941 cccctgatcg tatggatgaa ttggataatt ttctgcctga agacaaagct gcagcacgag
    12001 attttctcct tgaaatcaag ccactgtttc cgcgaataga gatccccaca ttctgggatg
    12061 atgacgaggg cgttgaactt ataaaacaac tttcctggta tatctctgca accgtcgtac
    12121 ttgcagactg gacagggtcg tcaacgcgat tttttccacg cgtcgcacag gcaatggata
    12181 ttgaagatta ctggcaaaag gctttagttc aggctcaaaa cgcattaacc gtctttccgc
    12241 cacaagcaga aaccgcgcct ttcaccggaa ttaatacgct gtttcctttt attgagaata
    12301 cgacaccgtt acagcaaaag gtgctggatc tggatatcaa tcagccagga ccacagttgt
    12361 ttattctgga agatgtgacc ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca
    12421 ggttgatggc tgcgggtaac gctcaggggc tgttttttgg cctgccaaca atggcaacgg
    12481 ccaatgccat gtacgatcga ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt
    12541 tccccgctcc agcggagata aaccggcgca atacggtgcg gttttcccgg aagtaaaccg
    12601 gacgcactgg atgcgatgat gggtatcact tagaattccc cgcccctgcg gtagaactcc
    12661 cagctcccat tttcaaaccc atcaagacgc cttcgccagc tccttcacca acggtagcat
    12721 tatccgcata acgtcacggc agcgacgttc tattcttcca ggaagtgcct tatcaatatg
    12781 ttgttgatta tccagtctta cgtcatgcca gctatttccc gccgggaagg caggtgtttt
    12841 tgcgcgttgc tgataaccat ccttatttcc aagattccag ttagtcgctt ccaccgaaag
    12901 tacagcaatg cccgctttgt cgaatatttc tgcgtcatta caacccccag tgcctttcgg
    12961 ataattttta ttcaaacccg gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc
    13021 cagcgccctg tcgcgcgtta atttccttac tgcttcaggg gtttttacac cgctgttgaa
    13081 atacaattta tcgccaacaa ttaagttatc gagattaatc accagcagcg tatttttctt
    13141 ttcggtgtca ctcatccgct tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc
    13201 gctggtcgcc acaaatcgaa taccatactc ggtaggcgta tttttcaggc gttctgccag
    13261 ttccagcatg acacctaaac ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc
    13321 gagattggca tctgcgtcag catcgctcag cggggcgtaa gtatccagat gcgccataat
    13381 gatgatctgc tgcggcgctt tgccttcatg agcggcaatc accgtacttc ctgtcacgtt
    13441 atgccagttc ttgcgattat cgcgggcggt ataaatatac cgactattaa atgtccgaat
    13501 atcactgcga taacccattt gctgaaactg ttggcgaata taatcggcag ataacatttc
    13561 tgctggagtt ccggtcatgc gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt
    13621 agcaaaatcg ccaggtttag gtgacgaggc gtgtacgggg agaataaagc atacgccgag
    13681 cgccagggca gcggtacggt ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg
    13741 caaatttttt taccgccata gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc
    13801 ctaaacgccc gaaatccggt gccttaagca ctttttgata ttagctttgc caaatcgtta
    13861 ttccgttaag gaactactca ttctaattgg taatttcatt cgttctctta cgctccctat
    13921 agtcgaaaca tctgatggca agaaaatagc ggtattgcaa aggaacggtt atggctcaaa
    13981 aacgacttac tcacctgcgg caactggagg cggaaagcat ccacattatt cgcgaggtgg
    14041 cggcagaatt ctcaaatccg gtgatgctct actctatcgg taaagattcc agcgtcatgc
    14101 tgcatctggc gcgcaaggcg ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg
    14161 ataccggctg gaaattccgc gagatgtatg agttccgcga tcgtacggcg aaagcctacg
    14221 gctgcgagct gctggtgcat aaaaacccgg aaggcgtggc gatggggatt aatccattcg
    14281 tgcacggcag cgcgaaacat accgatatta tgaaaactga aggcctgaaa caggcgctga
    14341 acaaatacgg ttttgatgcc gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg
    14401 ctaaagagcg aatctactct ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc
    14461 gcccggagct gtggcacaac tacaacgggc aaattaacaa aggcgaaagc atccgcgtct
    14521 tcccgctctc taactggacc gagcaggata tctggcaata catctggctg gaaaatatcg
    14581 acattgtgcc gctgtatctc gctgcggaac gtccggttct ggaacgcgac ggtatgttga
    14641 tgatgattga tgacaaccgt atcgacctgc aaccgggcga agtgatcaaa aaacggatgg
    14701 tgcgtttccg tacgctgggc tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa
    14761 cactgccgga aatcatcgaa gagatgctgg tttccaccac cagtgaacgt cagggtcgcg
    14821 tgattgaccg cgaccaggcg ggatccatgg agctgaaaaa acgtcagggg tatttttaag
    14881 atgaacaccg tacttgcaca acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt
    14941 gcgcaacaac ataaaagcct gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa
    15001 agtaccctga ttggtcgtct gctgcacgat acccgccaaa tctacgaaga tcagctctca
    15061 tcgctgcata acgacagtaa gcgtcacgga acccagggcg aaaagctgga tctggctctg
    15121 ctggtggacg gcctgcaagc tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc
    15181 tatttctcta ccgagaagcg taaatttatt atcgccgaca ccccagggca cgagcagtac
    15241 actcgcaata tggcgactgg cgcatcgaca tgtgaactgg cgatcttact gattgatgcc
    15301 cgtaaaggcg tgctcgatca aacccgtcgt cacagtttta tctccacact gttggggatc
    15361 aaacatctgg tcgtggcgat caacaaaatg gatctggtgg attacagtga agagacgttc
    15421 acccgtattc gtgaagatta tctgaccttt gccgggcagc tgccgggtaa tctggatatc
    15481 cgctttgtgc cgctctccgc actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg
    15541 ccgtggtaca gcggtctgac actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg
    15601 gtggatgccc agccaatgcg cttcccggtg cagtacgtta accgcccaaa tctcgatttt
    15661 cgtggctacg ccggaatgct ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg
    15721 ctgccctctg gtgtggaatc aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa
    15781 gaagtctttg ccggagaagc gatcaccctg gtgctgacgg atgagatcga tatcagccgt
    15841 ggtgatctgc tgctggcggc agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat
    15901 gtggtatgga tggcggaaca gccgctttcc ccgggccaga gttatgacat caaaattgcc
    15961 ggtaagaaga cgcgtgctcg tgttgatggc attcgttatc aggttgatat taataacctt
    16021 acccaacgcg aagttgaaaa cctgccgctg aacggcatcg gcctggtgga tctcactttt
    16081 gacgagccac tggtgttaga tcgctatcag caaaatccgg ttaccggtgg gctgattttt
    16141 atcgatcgcc tgagcaatgt gaccgtgggt gccggtatgg tgcacgagcc agttagccag
    16201 gcaacagctg cgtcatctga attcagtgca ttcgaactgg aattgaatgc cctggttcgt
    16261 cgccactttc cgcactgggg cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg
    16321 acgaaaacgt cgtctggcat agtcatccgg tcactgtgca acaacgcgaa ctacaccacg
    16381 gtcatcgtgg tgtagtgctg tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg
    16441 ccggggcgct ggaggaggcg ttacataaac tcagcgtcag tacgtatctg ctggatggcg
    16501 acaatgttcg ccacggatta tgcagcgatc tcggttttag cgatgccgat cgtaaagaga
    16561 atatccgtcg cgtcggtgaa gtggcgaatt tgatggttga agccggactg gtggtgctga
    16621 ccgcatttat ctcgccacac cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag
    16681 ggcgctttat tgaagtgttt gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa
    16741 aaggcttata taagaaagcg cgtgccggtg aactgcgcaa ctttacggga atagattccg
    16801 tttacgaagc gcctgaatcg gcagaaattc atctcaatgg tgaacaatta gtaacaaatt
    16861 tggtacagca attattagat ctgttgagac agaacgatat tatcagatcc tgagacacca
    16921 ccgggtttcc atgcccggtt cgtcaaagta acaggatcaa atatgcgtaa tagccataac
    16981 attacactaa caaataacga cagccttact gaggatgaag aaactacatg gtcactgcct
    17041 ggtgccgtgg tcggttttat ctcctggtta tttgcgctgg cgatgccgat gttgatttat
    17101 ggctctaaca cgctgttctt ctttctctac acctggcctt tctttctggc actgatgcct
    17161 gtcgcggtag tggtggggat tgcgctgcat tcattgatgg acggaaagct acgctacagt
    17221 attgttttca ccctggtgac agtaggcatt atgtttggcg cactgtttat gtggctactg
    17281 ggctaatttg tactttcttg tctcctctgt tcataattca aaccgtaact aataatgaga
    17341 ttatgttctg cacgccctgg gtatacgtaa caatgggcaa atgtggtaca tttgcccgcg
    17401 ttgtcgcggt atccccaaca gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg
    17461 cagtttttca gggggcagga tgggtaaact aacgctgctg ttgctggcta ttctggtctg
    17521 gctacagtat tcgctgtggt tcggtaagaa cggtatacat gactataccc gcgtcaatga
    17581 tgatgtggcg gcactgcaag ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt
    17641 tgccgaaatt gacgatctca atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga
    17701 actcagcatg accaggccgg gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg
    17761 cgcacagtct gcggggcaaa acaatcgata aatcagcccg ggaattaaca tggcaaccac
    17821 tcatttggat gtttgcgccg tggttccggc ggccggattt ggccgtcgaa tgcaaacgga
    17881 atgtcctaag caatatctct caatcggtaa tcaaaccatt cttgaacact cggtgcatgc
    17941 gctgctggcg catccccggg tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg
    18001 ttttgcacaa cttcctctgg cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga
    18061 gcgtgccgat tccgtgctgg caggtctgaa agccgctggc gatgtgcagt gggtattggt
    18121 gcatgacgcc gctcgtcctt gtttgcatca ggatgacctt gcgcgattgt tggcgttgag
    18181 cgaaaccagc cgcacgggag ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc
    18241 cgaaccgggc aaaaatgcca ttgctcatac cgttgatcgc aacggcttat ggcacgcgct
    18301 gacgccgcaa tttttccctc gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga
    18361 aggcgctacc attaccgacg aagcctcggc gctggaatat tgcggatttc atccgcagtt
    18421 ggtcgaaggc cgcgcggata acattaaagt cacgcgcccg gaagatttgg cactggccga
    18481 gttttacctc acccgaacca tccatcagga gaatacataa tgcgaattgg acacggtttt
    18541 gacgtacacg cctttggcgg tgaaggccca attatcattg gtggcgtacg cattccttac
    18601 gaaaaaggat tgctggcgca ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca
    18661 ttgcttggcg cggcggcgct gggggatatc ggcaaactgt tcccggatac cgatccggca
    18721 tttaaaggtg ccgacagccg cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag
    18781 ggttataccc ttggcaacgt cgatgtcact atcatcgctc aggcaccgaa gatgttgccg
    18841 cacattccac aaatgcgcgt gtttattgcc gaagatctcg gctgccatat ggatgatgtt
    18901 aacgtgaaag ccactactac ggaaaaactt ggatttaccg gacgtgggga agggattgcc
    18961 tgtgaagcgg tggcgctact cattaaggca acaaaatgat tgagtttgat aatctcactt
    19021 acctccacgg taaaccgcaa ggcaccgggc tgctgaaagc caatccggaa gactttgtgg
    19081 tggtggaaga tttgggcttt gagcctgatg gtgaaggtga gcatattctg gttagaatcc
    19141 tcaaaaacgg ctgcaatacc cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc
    19201 attcccgtga agtcagcttc gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt
    19261 tatgtgctcg cgtgccgggc aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct
    19321 gccaggtgct ggagtatgcg cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta
    19381 acgcctttac cctggttctg cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga
    19441 tcgatatttg cgtaaaaggt gtaccgaact acttcggtgc ccaacgtttt gggattggcg
    19501 gtagcaactt gcagggcgcg ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca
    19561 ataaacggag tttttggttg tcggcagccc gcagtgcgtt gtttaatcag attgttgctg
    19621 agcgcctcaa aaaagcagac gttaatcaag ttgttgacgg cgatgcgcta caattagccg
    19681 gacgtggtag ctggtttgta gcaaccaccg aagaactggc ggaattacag cgtcgcgtca
    19741 acgataaaga gctgatgata accgccgcat tgccgggcag tggcgaatgg ggaactcagc
    19801 gtgaagcgct ggcattcgaa caagcagctg tcgccgcaga aactgaatta caagctttac
    19861 tggtgcgcga aaaagttgaa gccgcgcgca gagcgatgct gctgtatccg caacaattaa
    19921 gctggaactg gtgggatgac gtcaccgtag agatccgttt ctggcttccg gcgggtagtt
    19981 ttgcaaccag cgttgtcagg gaacttatca acacaacagg tgattatgcg catattgctg
    20041 agtaattatg acggggtaca tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag
    20101 tttgctgacg ttcaggtggt cgcccccgat cgtaaccgca gcggcgcttc aaattctctg
    20161 acactggaat cctccctgcg cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg
    20221 ggaaccccga ccgattgtgt ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac
    20281 attgttgtgt ccggaattaa cgccgggccg aatctggggg atgatgttat ttattccggt
    20341 acggtagccg ccgcgatgga aggccgtcat ttaggttttc cggcgctggc cgtctcgctt
    20401 gacgggcata aacattacga cactgccgcg gcggtaacct gttcaatttt gcgcgcactg
    20461 tgtaaagagc cgctgcgcac cggacgtatt cttaatatta acgttcagga tttacccttg
    20521 gatcaaatca aaggtattcg cgtgacgcgc tgcggtacac gacatccggc agatcaggtg
    20581 atcccgcagc aagatccgcg cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa
    20641 tgtgatgccg gtccggggac cgattttgct gcggtagatg agggctatgt ctccatcacg
    20701 ccgctgcatg tggatttaac tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac
    20761 agcgtgggag ttggcacgca atggtaagca gacgcgtaca agcacttctg gatcaattac
    20821 gtgcgcaagg tattcaggat gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa
    20881 aattcgttga tgaagcgttt gaacaaaaag cctgggacaa tatcgccttg ccgataggtc
    20941 aggggcagac aatttcgcag ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga
    21001 cgccgcagtc gcgggtgctg gaaattggca ctggttcggg atatcaaacg gcaatcctgg
    21061 cgcatcttgt ccagcatgtt tgctcggttg aacggattaa aggcttgcag tggcaggcac
    21121 gtcgccgcct gaaaaatctt gatttacata atgtttcaac ccgtcatggt gatggatggc
    21181 aaggttggca ggcacgtgcg ccgtttgacg ctatcattgt tacggcggca ccgccggaaa
    21241 ttccaactgc gctaatgacg cagctggacg aaggcgggat tctcgtctta cccgtagggg
    21301 aggagcacca gtatttgaaa cgggtgcgtc gtcggggagg cgaatttatt atcgataccg
    21361 tggaggccgt gcgctttgtc cctttagtga agggtgagct ggcttaaaac gtgaggaaat
    21421 acctggattt ttcctggtta ttttgccgta ggtcagcgta tcgtgaacat cttttccagt
    21481 gttcagtagg gtgccttgca cggtaattat gtcactggtt attaaccaat ttttcctggg
    21541 ggataaatga gcgcgggaag cccaaaattc accgttcgcc gcattgcggc tttgtcactg
    21601 gtttcgctat ggctggcagg ctgttctgac acttcaaatc caccggcacc ggtcagctcc
    21661 gttaatggca atgcgcctgc aaatactaat tctggtatgt tgattacgcc gccgccgaaa
    21721 atggggacga cgtctacagc gcagcaaccg caaattcagc cggtacagca gccacaaatt
    21781 caggctactc aacaaccgca aatccagcca gtgcagccag tagctcagca gccggtacag
    21841 atggaaaacg gacgcatcgt ctataaccgt cagtatggga acattccgaa aggcagttat
    21901 agcggcagta cctataccgt gaaaaaaggc gacacacttt tctatatcgc ctggattact
    21961 ggcaacgatt tccgtgacct tgctcagcgc aacaatattc aggcaccata cgcgctgaac
    22021 gttggtcaga ccttgcaggt gggtaatgct tccggtacgc caatcactgg cggaaatgcc
    22081 attacccagg ccgacgcagc agagcaagga gttgtgatca agcctgcaca aaattccacc
    22141 gttgctgttg cgtcgcaacc gacaattacg tattctgagt cttcgggtga acagagtgct
    22201 aacaaaatgt tgccgaacaa caagccaact gcgaccacgg tcacagcgcc tgtaacggta
    22261 ccaacagcaa gcacaaccga gccgactgtc agcagtacat caaccagtac gcctatctcc
    22321 acctggcgct ggccgactga gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc
    22381 aacaagggga ttgatatcgc aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc
    22441 cgcgttgttt atgctggtaa cgcgctgcgc ggctacggta atctgattat catcaaacat
    22501 aatgatgatt acctgagtgc ctacgcccat aacgacacaa tgctggtccg ggaacaacaa
    22561 gaagtgaagg cggggcaaaa aatagcaacc atgggtagca ccggaaccag ttcaacacgc
    22621 ttgcattttg aaattcgtta caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag
    22681 cgataaatcg gcggaaccag gcttttgctt gaatgttccg tcaagggatc acgggtagga
    22741 gccaccttat gagtcagaat acgctgaaag ttcatgattt aaatgaagat gcggaatttg
    22801 atgagaacgg agttgaggtt tttgacgaaa aggccttagt agaagaggaa cccagtgata
    22861 acgatttggc cgaagaggaa ctgttatcgc agggagccac acagcgtgtg ttggacgcga
    22921 ctcagcttta ccttggtgag attggttatt caccactgtt aacggccgaa gaagaagttt
    22981 attttgcgcg tcgcgcactg cgtggagatg tcgcctctcg ccgccggatg atcgagagta
    23041 acttgcgtct ggtggtaaaa attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg
    23101 accttatcga agagggcaac ttggggctga tccgcgcggt agagaagttt gacccggaac
    23161 gtggtttccg cttctcaaca tacgcaacct ggtggattcg ccagacgatt gaacgggcga
    23221 ttatgaacca aacccgtact attcgtttgc cgattcacat cgtaaaggag ctgaacgttt
    23281 acctgcgaac cgcacgtgag ttgtcccata agctggacca tgaaccaagt gcggaagaga
    23341 tcgcagagca actggataag ccagttgatg acgtcagccg tatgcttcgt cttaacgagc
    23401 gcattacctc ggtagacacc ccgctgggtg gtgattccga aaaagcgttg ctggacatcc
    23461 tggccgatga aaaagagaac ggtccggaag ataccacgca agatgacgat atgaagcaga
    23521 gcatcgtcaa atggctgttc gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat
    23581 tcggtttgct ggggtatgaa gcggcaacac tggaagatgt aggtcgtgaa attggcctca
    23641 cccgtgaacg tgttcgccag attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc
    23701 aaacgcaggg gctgaatatc gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag
    23761 gccagtctca agcgaggctg gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc
    23821 tggataagac acgtcagcgt cctacccggc aatcagcatt ctatgtgctc atcatcttgc
    23881 gcaccaactg cataaacagc tcctgctctt ctccactcaa acgccccaaa aattcctcat
    23941 ccacgctgtc accaataggt atcgccgcag caagtagctt ttctccttcg gcagtcagcc
    24001 agacaaagcg tcgccgcttg tcagcgacgt catgttcacg tctgactaag ccacgatttt
    24061 ccattcttgc caacatttct gctagcgttg ctttggtgct gactgctgct tctatcagtg
    24121 ccacctgttc aataccaggt ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg
    24181 tcaggtcagg taactcgtgc tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact
    24241 gatggaacgc tttatttcgt aacgccatgt aaactccggg ctaattctct gtcgctatca
    24301 tagcggtttt ttagtgacga agaagatgtt gttacttttc aatccttgcc gctgtggaaa
    24361 tgaaggagta tgttaataat aacgttcgta tacgaataat taagagaata agcaatgaaa
    24421 ctgatcgtcg ggatgacagg ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg
    24481 ctgcgtgaga tgccgaatgt cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc
    24541 attgaactgg aaacgcctta cagcgctcgc gatgttgctg ccctcgcaga cttcagccat
    24601 aacccggcgg atcaggcggc gatcatctca tccggttctt ttcgtaccga cggcatgatc
    24661 gttattccgt gcagtatgaa aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg
    24721 gtagggcgcg cggcggacgt cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt
    24781 gaaatgccgc ttagcaccat ccatctcgaa aatatgctcg cactttcacg catgggcgtg
    24841 gcgatggtgc cgccgatgcc tgccttttat aaccatcccg aaacggtaga tgacattgtc
    24901 caccatgtgg tagcccgcgt gctggatcaa tttggcctcg aacatcccca cgccaggcgc
    24961 tggcaaggat tgccgcaggc ccggaatttt tctcaggaga atgaataatg gcatttgatg
    25021 atttacgcag ctttttacag gcgcttgatg accacggcca gttactgaaa atcagcgaag
    25081 aagtgaacgc tgagccggat ctggcagcag cagctaacgc caccgggcgt atcggcgacg
    25141 gcgcgcccgc gctgtggttt gataatattc gcggctttac cgatgcccgc gtggcgatga
    25201 ataccatcgg ttcctggcag aaccacgcga tttcccttgg cctgccacca aacaccccgg
    25261 tcaaaaagca gattgatgag tttatccgcc gctgggataa cttcccgatt gccccggagc
    25321 gccgcgccaa tccagcctgg gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg
    25381 atattctgcc gctgtttcgc ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt
    25441 gcgtggtttc ccgcgatccg ctcgacccgg ataacttcgg caagcagaac gtcggcatct
    25501 accgcatgga agtgaagggc aagcgtaagc tcggcctgca accggtgccg atgcacgata
    25561 tcgccctgca tctgcataaa gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc
    25621 tcggtaacga tccgatcatc acgctgatgg gggccacgcc gctgaaatac gatcagtctg
    25681 agtatgaaat ggcaggcgcg ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga
    25741 ccggttttga tgtgccgtgg ggttcagaag tgatcctcga aggggttatc gaaagccgta
    25801 aacgcgaaat cgaagggccg ttcggtgagt ttaccgggca ctactccggt gggcgcaaca
    25861 tgaccgtggt gcgcatcgat aaagtctctt atcgcagcaa accgattttc gaatcactct
    25921 atctcggtat gccgtggacc gaaatcgact acctatgact aggtaattta ttcaagataa
    25981 ccgggaagag attaaaaaag acgggaatag attggaatca acaagttagg aactcaaggt
    26041 tttcaagata aagcgagtcc aaaaatgtta aacggttcga tatcaataaa acattcaaga
    26101 taactcacag gttcttacaa tagcctttaa agaccgttca aaaacgattc aggtgtttat
    26161 tatagcgctg atgggtgcaa gcgccaaaac caatgtaatt ttagcacagc taggcacttg
    26221 cactgggtta tgcgctggct gctttctgag gtagatttaa gtgatcgccc ttagcttcgg
    26281 acaacgcgta aatctctcta accttttcgt ctggcaaatt accaaggctt aacgctatgg
    26341 ctctacgaac tccaactaga gcgtgcaact cttggatgtg ctcatccaga agaatgagta
    26401 attgctccgc tccttttctc ccaagaacat cagctacttg ttgtttttct tgtggtttaa
    26461 gaaatgacaa gaacattaga atttgagcat caacattatc cgcattctct tttttgggtt
    26521 ctttggaatc ttttagttca acatcaccct ctcccgtcat gagccattcc aaggtaacat
    26581 tttcgctttc tgctatcttt tttaactgat acgggcgtgg tatcggtgcg taatcccgcc
    26641 tgttgtaata attttttaaa gtgccttcat tgatccccca tgcccgagca gccgcatttc
    26701 ttgaagggta tcgttctact aacctaaaga ttctttcgat aatcggttct tttccatcgg
    26761 caggaaaaga accttggggt gcttttctca ttcttggttc ctttctatct aattcattga
    26821 taaataataa aattaaaaaa taaaccataa aaacaccaaa gaaccctttg cagggaatct
    26881 ttggtatctt aatctttatc catacggata tccgaccgga tatccgcgcg ggtaattttg
    26941 taggttaaag gaaagatgag acaaagagaa gatagtgacc gcgattggcc ttcgctgaag
    27001 attcagctgg cgctcaaaga taacgggtgg tcttccttcc gggagctttc actccatgct
    27061 ggttatgctc aggattcact gaaagcggta gttcgcacac caaacaaaaa atacgaacgc
    27121 gtaatttctg acgctatagg tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac
    27181 gatggattta tcaggaaggc gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct
    27241 cggcgtacca ggaatgccag ccaccacaaa aggcatccga caggcgttac agcgtttttc
    27301 tcgtgatctt ggtgacgtta gtcgccgtcg ggaaggcaca aaagccattg agtaccacat
    27361 tgattgtctg cctgaaataa cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt
    27421 ggctacagag aacaatgttt ctgaagtgaa agctgttacc agaaaaacac gcaatcctga
    27481 cgctgtccag gcaatcgaag catatcgcgg ttctccacaa ctgatggaag aacgcctgaa
    27541 tgcgctgacc gaaaaccagc gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt
    27601 gctgaagctg gaaagcgccg gtaaccccgg gcgactgaaa gccattaact ttcttgttga
    27661 aaaagcccgt aaaggtgagc tgccggagcg cctgcaacag gccgcagtta acgccaatgc
    27721 aaaacgtggc gctaatcgta ctatcagccg cgatccactt taccagtggg ttctgaaata
    27781 caaccagtcg cagaacgccg ccgaacggct gctgttactg gcaccgggaa aacgcgacga
    27841 aataaaaccg gaagaaatca gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa
    27901 cggcagaccc atgtcagaag cctacgagga ttttgtcgca gaatggcagc gtcgccatgc
    27961 ggacgaaccg tacatgctgg aggtgatgcc ttcctatgat gttgtccggt atgccatgaa
    28021 aaaactgccg gaagtggtga aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct
    28081 tgaaggattc acccgccgcg actggacagc tatgccggtg aattatgtct ggattggtga
    28141 cggtcacggt atgaagctga aatgtgcgca tccgatccac gggcgtccat tctcaccgga
    28201 agtcacgttt gtgatcgatg gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc
    28261 cgaaaacgtg ttcgccgttg ccggtgcgat acagcacggc attcgcaacc acggaaaacc
    28321 gttcctgtat tactcggata acggttccgg tgaaacggct gacatgctgg acaaagaggt
    28381 cgtgggtatt ctgccacgcc tggggattaa acaccctacc ggtattgccg gtaatccaca
    28441 gggacgcggc attattgaac gcctgaaccg gacactgccg atgcgtatag cgaggaggta
    28501 tcgcacctat tttggcaagg gtgctgaccg ggaatcgttg cgtgtactta accgtgatct
    28561 gcgttcagcg tttaacgccc tgcaacagga caagcctctg aacgaccgcc agaaagcggc
    28621 aatgcgcgag ctgccatcgt gggcggagct tattgaggct attcgtgaag gcgtggaatg
    28681 gtacaacaac cgcccgcatt ctgagctgcc gatgaaaccg aacggacggc attacagccc
    28741 gacagagttc agaaaaaaac gccaggcaga agaagacacc gaaattgagt ggctgagtga
    28801 tcttgagctg cgcgacatgt tccgcccaat ggtggaacga ccggtaagac gctgcgaaat
    28861 tcagtggctg aacaatattt actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt
    28921 gcttatcagt tatgacattc atgacgccga gcgaattacg gtacgtcgca aggacggcag
    28981 ctttatctgt gaggcgatat ggaacggcaa taaacgcgca gcctttgctg tcagtgctga
    29041 atatcacaaa caacagcagc gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg
    29101 tgatgctgaa gatgaaggta ttcagattct ggaacacaaa caggctgaac cctggctgag
    29161 taatgtttat cgcccggttg gtaatgtggt ggcagttcag caaccggaat atgaagaaga
    29221 gcatgacgaa gaatttgaac gcgatttccg gctgg
//
LOCUS       DAJTUP010000041        29172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTUP010000041 DAJTUP010000000
VERSION     DAJTUP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.041"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..203)
                     /locus_tag="OO468_001902"
     CDS             complement(<1..203)
                     /locus_tag="OO468_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR6259036.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
     gene            513..2285
                     /gene="aspS"
                     /locus_tag="OO468_001903"
     CDS             513..2285
                     /gene="aspS"
                     /locus_tag="OO468_001903"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR6259037.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2403..2855
                     /gene="nudB"
                     /locus_tag="OO468_001904"
     CDS             2403..2855
                     /gene="nudB"
                     /locus_tag="OO468_001904"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR6259038.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2884..3624
                     /locus_tag="OO468_001905"
     CDS             2884..3624
                     /locus_tag="OO468_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6259039.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3659..4180
                     /gene="ruvC"
                     /locus_tag="OO468_001906"
     CDS             3659..4180
                     /gene="ruvC"
                     /locus_tag="OO468_001906"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR6259040.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4182..4784)
                     /locus_tag="OO468_001907"
     CDS             complement(4182..4784)
                     /locus_tag="OO468_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR6259041.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4854..4919
                     /gene="yobI"
                     /locus_tag="OO468_001908"
     CDS             4854..4919
                     /gene="yobI"
                     /locus_tag="OO468_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR6259042.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5058..5669
                     /gene="ruvA"
                     /locus_tag="OO468_001909"
     CDS             5058..5669
                     /gene="ruvA"
                     /locus_tag="OO468_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR6259043.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5678..6688
                     /gene="ruvB"
                     /locus_tag="OO468_001910"
     CDS             5678..6688
                     /gene="ruvB"
                     /locus_tag="OO468_001910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR6259044.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO468_001911"
     CDS             complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO468_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR6259045.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO468_001912"
     CDS             complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO468_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR6259046.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8451..9383
                     /gene="znuA"
                     /locus_tag="OO468_001913"
     CDS             8451..9383
                     /gene="znuA"
                     /locus_tag="OO468_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR6259047.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9399..10721
                     /gene="mepM"
                     /locus_tag="OO468_001914"
     CDS             9399..10721
                     /gene="mepM"
                     /locus_tag="OO468_001914"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR6259048.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10841..11812
                     /gene="lpxM"
                     /locus_tag="OO468_001915"
                     /gene_synonym="msbB"
     CDS             10841..11812
                     /gene="lpxM"
                     /locus_tag="OO468_001915"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6259049.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO468_001916"
     CDS             complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO468_001916"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR6259050.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO468_001917"
                     /gene_synonym="yebK"
     CDS             complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO468_001917"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR6259051.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14719..16194
                     /gene="zwf"
                     /locus_tag="OO468_001918"
     CDS             14719..16194
                     /gene="zwf"
                     /locus_tag="OO468_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR6259052.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16429..18240
                     /gene="edd"
                     /locus_tag="OO468_001919"
     CDS             16429..18240
                     /gene="edd"
                     /locus_tag="OO468_001919"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR6259053.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18277..18918
                     /gene="kdgA"
                     /locus_tag="OO468_001920"
                     /gene_synonym="eda"
     CDS             18277..18918
                     /gene="kdgA"
                     /locus_tag="OO468_001920"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR6259054.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO468_001921"
     CDS             complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO468_001921"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR6259055.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20286..20576
                     /gene="yebG"
                     /locus_tag="OO468_001922"
                     /pseudo
     CDS             20286..20576
                     /gene="yebG"
                     /locus_tag="OO468_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20643..20999
                     /locus_tag="OO468_001923"
     CDS             20643..20999
                     /locus_tag="OO468_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR6259056.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21326..21985
                     /locus_tag="OO468_001924"
     CDS             21326..21985
                     /locus_tag="OO468_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR6259057.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22195..24255
                     /gene="ptrB"
                     /locus_tag="OO468_001925"
     CDS             22195..24255
                     /gene="ptrB"
                     /locus_tag="OO468_001925"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR6259058.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO468_001926"
     CDS             complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO468_001926"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR6259059.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24938..25594)
                     /locus_tag="OO468_001927"
                     /pseudo
     CDS             complement(24938..25594)
                     /locus_tag="OO468_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO468_001928"
     CDS             complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO468_001928"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR6259060.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26065..26439
                     /gene="yobA"
                     /locus_tag="OO468_001929"
     CDS             26065..26439
                     /gene="yobA"
                     /locus_tag="OO468_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR6259061.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26443..27315
                     /gene="copD"
                     /locus_tag="OO468_001930"
                     /pseudo
     CDS             26443..27315
                     /gene="copD"
                     /locus_tag="OO468_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27328..27669
                     /locus_tag="OO468_001931"
     CDS             27328..27669
                     /locus_tag="OO468_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR6259062.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28062..29139)
                     /locus_tag="OO468_001932"
                     /pseudo
     CDS             complement(28062..29139)
                     /locus_tag="OO468_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
BASE COUNT         7370 a         7168 c         7502 g         7132 t
ORIGIN      
        1 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
       61 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      121 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      181 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      241 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      301 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      361 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      421 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      481 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
      541 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
      601 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
      661 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
      721 aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
      781 gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc
      841 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
      901 gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
      961 gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc
     1021 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     1081 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt gaccgttact
     1141 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     1201 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     1261 cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     1321 tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga
     1381 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcggggc
     1441 cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga
     1501 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     1561 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     1621 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     1681 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg ggcgcactgc
     1741 tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     1801 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     1861 cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg gaaaatgcgg
     1921 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     1981 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     2041 agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg ccgcacgcag
     2101 gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac aatatccgtg
     2161 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     2221 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     2281 actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa
     2341 ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca
     2401 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag
     2461 atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa
     2521 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     2581 aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag
     2641 agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg
     2701 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     2761 cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc
     2821 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     2881 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     2941 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     3001 ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac
     3061 aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     3121 gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     3181 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc attcagcaaa
     3241 tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa aaaaggcgtg
     3301 atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga agcaggtgct
     3361 gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     3421 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     3481 atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact gatgcgtctg
     3541 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     3601 gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga gacgcgtgat
     3661 ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     3721 ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag tggatgattt
     3781 accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     3841 tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact
     3901 gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc cggtatttga
     3961 atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg aaaaaagcca
     4021 ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg cggatgccgc
     4081 cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga tgcagatgag
     4141 cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc ctcggtcagt
     4201 tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg atgatgccac
     4261 aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa ccagaatttc
     4321 cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt ctctatttca
     4381 cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga acagaactgg
     4441 cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga gggaacctgt
     4501 tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt tatagcatag
     4561 cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga aaggggaacc
     4621 cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc gacgtgatta
     4681 ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat acatgtaaag
     4741 acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc attccattga
     4801 aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc gcaatgtata
     4861 tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt ccaatataac
     4921 ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg caagttttct
     4981 tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat ctttgattca
     5041 ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc
     5101 cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt
     5161 tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg
     5221 aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg ttcaaagagt
     5281 tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc ggaatgtcag
     5341 cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg aaactgccgg
     5401 gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt
     5461 tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct gctagcccgg
     5521 cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc tataaaccac
     5581 aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt gaaacattaa
     5641 ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat
     5701 ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc ctaaattact
     5761 ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc
     5821 gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg ggttgggtaa
     5881 aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg
     5941 tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg aaccgcatga
     6001 cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag tgctgtaccc
     6061 ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat
     6121 taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag gttcgctgac
     6181 atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga
     6241 tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga gtgatgacgg
     6301 tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg
     6361 tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc
     6421 tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt
     6481 gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc
     6541 agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt tgattcagca
     6601 aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt ggaatcactt
     6661 tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg catccgacac
     6721 tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga gatagcgaac
     6781 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga tagattagct
     6841 ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca ccaccgacgg
     6901 acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc
     6961 caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc
     7021 tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc
     7081 aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt tcacaccatc
     7141 aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa
     7201 aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc
     7261 cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca tcagactaac
     7321 gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc
     7381 cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat
     7441 caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc
     7501 atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg
     7561 acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat
     7621 gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atgattatga
     7681 tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc
     7741 ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc
     7801 acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa
     7861 tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc atttacatcc
     7921 acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa tgctcgcgct
     7981 aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc
     8041 ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag
     8101 cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca
     8161 tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg
     8221 agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt
     8281 tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc
     8341 gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga
     8401 aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata
     8461 aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg
     8521 cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg
     8581 atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat gattattcac
     8641 tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg
     8701 agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag caggtaacga
     8761 ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg
     8821 atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc
     8881 tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg
     8941 aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat
     9001 tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt
     9061 tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc
     9121 attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac
     9181 agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg
     9241 ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga
     9301 atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg
     9361 cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg
     9421 tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg
     9481 ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat gccacgccaa
     9541 ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg
     9601 agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca
     9661 aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca
     9721 gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct gctgccgaca
     9781 aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg
     9841 aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg
     9901 ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc
     9961 tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg
    10021 ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa
    10081 aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa
    10141 gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca atccgcgctg
    10201 aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc
    10261 cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact aatccggtga
    10321 caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt acgccagtgc
    10381 tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg
    10441 tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg
    10501 tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac
    10561 gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc
    10621 tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg
    10681 cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc
    10741 ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc cgctacacta
    10801 tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa
    10861 tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg
    10921 gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat
    10981 tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt
    11041 aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga
    11101 tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg
    11161 gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg aagagatgcg
    11221 gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc
    11281 catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc
    11341 ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag
    11401 aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt
    11461 acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa
    11521 agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg ttgtaccgct
    11581 gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc caccgatgga
    11641 tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag aagtcgagat
    11701 ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga aaactcgcaa
    11761 accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat aaaaaagcct
    11821 ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa tattcgaaat
    11881 ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat ccggcaacgt
    11941 acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact catcacgtcg
    12001 ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg cagcagatta
    12061 accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac cggcgtaacg
    12121 ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc gaaaattggt
    12181 agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt catggtaatg
    12241 atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc aatagcttct
    12301 tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct cgggattttt
    12361 tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata ctgcccagcg
    12421 gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt tgctacgtcc
    12481 atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat ctgggtcgcc
    12541 gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg aatgccgacc
    12601 agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac cacgtcagag
    12661 gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg ttcaaccttg
    12721 gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata gttcagatct
    12781 tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc cgcagtctta
    12841 atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc aagtttgttg
    12901 atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac tttcatgccc
    12961 tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat gtcaccaggc
    13021 acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc gccttcacct
    13081 ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac tttgccttct
    13141 ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc cacatgacgc
    13201 cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc ttcaggcgag
    13261 ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt ttcaagatta
    13321 ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg aagccttttg
    13381 gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga aatccgaatg
    13441 aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag attaggacag
    13501 tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa aacgcgattc
    13561 cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc gtcgcaaagt
    13621 aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa ccatgggcat
    13681 ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt cccgggcgag
    13741 cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg ccagctgcgc
    13801 cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca cgtctccgtc
    13861 gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca ccaccggaac
    13921 attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag agcctaatcc
    13981 gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga tggcagattt
    14041 atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata ttttccccgt
    14101 gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag tgccattcgc
    14161 cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca tgctgcgaca
    14221 gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag caatactcga
    14281 atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct ctgatttgct
    14341 caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa gagactcacg
    14401 ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga tattacgcct
    14461 gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca taacatgatc
    14521 agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg ccgcgctttg
    14581 cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca agctcgtaaa
    14641 agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta ccgggttagt
    14701 taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga cctggtcatt
    14761 ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta tcaactggaa
    14821 aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc tgactgggat
    14881 aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa agaaaccatt
    14941 gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct cgatgtcaat
    15001 gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg tatcactatt
    15061 aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct tggcgaggca
    15121 aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac gtcgctggcg
    15181 acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca ggtttaccgt
    15241 atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg ttttgctaac
    15301 tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat taccgtggca
    15361 gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca gatgcgtgac
    15421 atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc gccatctgac
    15481 ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct gcgccgcatc
    15541 gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg cttcgcccag
    15601 ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag caatacagaa
    15661 accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt gccattctac
    15721 ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta tttcaaaaca
    15781 cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa actgactatc
    15841 cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc tggccttgac
    15901 cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac ctttaatcag
    15961 acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg tattcaggca
    16021 ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc cattactgag
    16081 gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg gggacccgtt
    16141 gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga gtaatatcag
    16201 cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg tagaacaatt
    16261 tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta cactgactga
    16321 aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc tctgaatgaa
    16381 acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat gaatccacaa
    16441 ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc tgcttatctc
    16501 gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc atgcggtaac
    16561 ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa aagcatgttg
    16621 cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca ccagccttat
    16681 gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt tggtcaggtt
    16741 gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg aatggaattg
    16801 tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc ccataacatg
    16861 tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct gacgatggca
    16921 gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat ggcaagcggt
    16981 ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa agtggaccgc
    17041 atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg tactttctac
    17101 ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt gccaggctct
    17161 tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag
    17221 gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat gatcgatgag
    17281 aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac taaccacacc
    17341 atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga tgacttctct
    17401 gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc ggccgatatt
    17461 aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct caaagcaggc
    17521 ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta tacccttgaa
    17581 ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc actcgacagc
    17641 aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa agtgttaagc
    17701 ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt
    17761 gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt tgaagcgggt
    17821 ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg
    17881 ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg tttcaaaatt
    17941 gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc agctatccac
    18001 gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt
    18061 cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga actggctgct
    18121 cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg tgaattattc
    18181 agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat cactttttaa
    18241 gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa aacaagtgca
    18301 gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa actggaacac
    18361 gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga agtgactctg
    18421 cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt
    18481 gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga agcgggtgca
    18541 cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac cgaagggact
    18601 attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat ggactacggt
    18661 ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg
    18721 atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc tccggctaac
    18781 taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg
    18841 gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg tgaagctgta
    18901 gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt tttgtagcgt
    18961 ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa
    19021 tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg ctgccatcaa
    19081 tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc tgcacattat
    19141 caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct gcaggaccat
    19201 actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg gcaaactctg
    19261 agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg gagacctcac
    19321 tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca taaccgccca
    19381 gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc gggctcattt
    19441 gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct actggtgcac
    19501 agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa
    19561 cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga gcgtattccc
    19621 atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg gaagagctca
    19681 tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt
    19741 tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc gccaggcgac
    19801 ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca acattcagtc
    19861 cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc ggcacgatat
    19921 aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc atattaatga
    19981 catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg acggcaatca
    20041 cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca ccggagccta
    20101 ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc ataacgaata
    20161 tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg
    20221 atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat tatgagaggc
    20281 tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt
    20341 ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg
    20401 acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc
    20461 tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc aaattaccgt
    20521 ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca
    20581 acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa
    20641 acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc gcatcagttt
    20701 tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg
    20761 ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg gcgcgctggg
    20821 cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt ttgcaggaca
    20881 ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa agtgccgata
    20941 ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag cggcgttaat
    21001 ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg tccgtcccag
    21061 aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc aatcagcatc
    21121 aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat cacctgatat
    21181 acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc cccgcttatt
    21241 tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg ctacaggcac
    21301 taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc aatccctgct
    21361 tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca aactgttagt
    21421 gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag cacgtaaact
    21481 tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag cgggtacggt
    21541 gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac cgcagtttgg
    21601 cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc tggtctttgc
    21661 cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc agcaattgcg
    21721 tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg aacaaccgct
    21781 ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg aaatctattt
    21841 cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc tgaatgcact
    21901 gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg atctcgaaca
    21961 gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt tgactatttc
    22021 gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct gcataacatt
    22081 cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt cgcttgcatc
    22141 cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa taacatgcta
    22201 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
    22261 tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta cctgcaacaa
    22321 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
    22381 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
    22441 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
    22501 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
    22561 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
    22621 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
    22681 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
    22741 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
    22801 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
    22861 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
    22921 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
    22981 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
    23041 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
    23101 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
    23161 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
    23221 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
    23281 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
    23341 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
    23401 caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
    23461 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
    23521 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
    23581 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
    23641 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
    23701 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
    23761 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
    23821 aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
    23881 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtggggaaat
    23941 ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
    24001 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
    24061 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
    24121 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
    24181 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
    24241 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
    24301 atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc ggcaacgtcg
    24361 gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga cggacgtccg
    24421 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
    24481 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag cggtgtctgt
    24541 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
    24601 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
    24661 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
    24721 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
    24781 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
    24841 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
    24901 ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga atgatatcgc
    24961 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
    25021 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
    25081 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
    25141 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
    25201 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
    25261 tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa
    25321 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag gtcgttgcgg
    25381 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
    25441 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
    25501 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
    25561 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
    25621 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
    25681 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
    25741 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
    25801 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
    25861 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
    25921 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
    25981 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
    26041 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
    26101 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
    26161 cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac tttaaacttc
    26221 tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat
    26281 attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat tgttccactg
    26341 gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg
    26401 cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg
    26461 atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt tgcaatgtat
    26521 ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc tctgcgttta
    26581 caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc
    26641 gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg
    26701 caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc
    26761 gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa
    26821 tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac agcgaaaatc
    26881 catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg
    26941 ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc tattcaggcg
    27001 ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg
    27061 cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc
    27121 ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc taatcgttat
    27181 gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg
    27241 actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact
    27301 ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta
    27361 ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag tcgttttgaa
    27421 gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg ccgaccgggt
    27481 aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa cgatatcgca
    27541 caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat
    27601 gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa
    27661 ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt
    27721 caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt tattgaccat
    27781 tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca aggcaactaa
    27841 gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt tccctggacc
    27901 gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt agttgcaaac
    27961 gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt ttttggttat
    28021 atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag taagtacgtt
    28081 ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc aagtagacgc
    28141 tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct tctgatttcg
    28201 tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg ctgcgacaaa
    28261 tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc taatcccggc
    28321 actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg caccaagtcg
    28381 taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc ctgtcttaat
    28441 ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg ttatgtcttc
    28501 gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg ccggaagttg
    28561 atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc ttgtaactac
    28621 tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt caacaatggc
    28681 ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt ttttccctcc
    28741 ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg tcagtaaaac
    28801 ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat aagtatttct
    28861 ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg ccatagtaaa
    28921 tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt tgtatatata
    28981 atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac ctagtgtaaa
    29041 ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc gatatgttaa
    29101 gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc tccattaacg
    29161 gactgtctct cc
//
LOCUS       DAJTUP010000042        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTUP010000042 DAJTUP010000000
VERSION     DAJTUP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.042"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            107..1138
                     /gene="mdtN"
                     /locus_tag="OO468_001933"
     CDS             107..1138
                     /gene="mdtN"
                     /locus_tag="OO468_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR6259063.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1138..3180
                     /gene="mdtO"
                     /locus_tag="OO468_001934"
     CDS             1138..3180
                     /gene="mdtO"
                     /locus_tag="OO468_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR6259064.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3177..4643
                     /gene="mdtP"
                     /locus_tag="OO468_001935"
     CDS             3177..4643
                     /gene="mdtP"
                     /locus_tag="OO468_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR6259065.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4841..5080
                     /locus_tag="OO468_001936"
     CDS             4841..5080
                     /locus_tag="OO468_001936"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259066.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5090..5260
                     /locus_tag="OO468_001937"
     CDS             5090..5260
                     /locus_tag="OO468_001937"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6259067.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5309..6721
                     /gene="fdhF"
                     /locus_tag="OO468_001938"
     CDS             5309..6721
                     /gene="fdhF"
                     /locus_tag="OO468_001938"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR6259068.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6722..6988
                     /locus_tag="OO468_001939"
     CDS             6722..6988
                     /locus_tag="OO468_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR6259069.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7082..7771
                     /locus_tag="OO468_001940"
     CDS             7082..7771
                     /locus_tag="OO468_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6259070.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7849..9162)
                     /gene="gltP"
                     /locus_tag="OO468_001941"
     CDS             complement(7849..9162)
                     /gene="gltP"
                     /locus_tag="OO468_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR6259071.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9504..10100)
                     /gene="nrfG"
                     /locus_tag="OO468_001942"
     CDS             complement(9504..10100)
                     /gene="nrfG"
                     /locus_tag="OO468_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR6259072.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10097..10480)
                     /gene="nrfF"
                     /locus_tag="OO468_001943"
     CDS             complement(10097..10480)
                     /gene="nrfF"
                     /locus_tag="OO468_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR6259073.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10473..12191)
                     /locus_tag="OO468_001944"
     CDS             complement(10473..12191)
                     /locus_tag="OO468_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR6259074.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12211..13167)
                     /gene="nrfD"
                     /locus_tag="OO468_001945"
     CDS             complement(12211..13167)
                     /gene="nrfD"
                     /locus_tag="OO468_001945"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR6259075.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13164..13835)
                     /gene="nrfC"
                     /locus_tag="OO468_001946"
     CDS             complement(13164..13835)
                     /gene="nrfC"
                     /locus_tag="OO468_001946"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR6259076.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13832..14398)
                     /gene="nrfB"
                     /locus_tag="OO468_001947"
     CDS             complement(13832..14398)
                     /gene="nrfB"
                     /locus_tag="OO468_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR6259077.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14443..15879)
                     /gene="nrfA"
                     /locus_tag="OO468_001948"
     CDS             complement(14443..15879)
                     /gene="nrfA"
                     /locus_tag="OO468_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR6259078.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16272..18230
                     /gene="acs"
                     /locus_tag="OO468_001949"
                     /pseudo
     CDS             16272..18230
                     /gene="acs"
                     /locus_tag="OO468_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18430..18744
                     /locus_tag="OO468_001950"
     CDS             18430..18744
                     /locus_tag="OO468_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR6259079.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18741..20390
                     /gene="actP"
                     /locus_tag="OO468_001951"
     CDS             18741..20390
                     /gene="actP"
                     /locus_tag="OO468_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR6259080.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20568..21860
                     /locus_tag="OO468_001952"
     CDS             20568..21860
                     /locus_tag="OO468_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR6259081.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22014..23663)
                     /locus_tag="OO468_001953"
     CDS             complement(22014..23663)
                     /locus_tag="OO468_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR6259082.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23814..25163)
                     /gene="ghxP"
                     /locus_tag="OO468_001954"
                     /gene_synonym="yjcD"
     CDS             complement(23814..25163)
                     /gene="ghxP"
                     /locus_tag="OO468_001954"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR6259083.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25869..26333)
                     /gene="soxR"
                     /locus_tag="OO468_001955"
     CDS             complement(25869..26333)
                     /gene="soxR"
                     /locus_tag="OO468_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR6259084.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26419..26742
                     /gene="soxS"
                     /locus_tag="OO468_001956"
     CDS             26419..26742
                     /gene="soxS"
                     /locus_tag="OO468_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR6259085.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26745..28322)
                     /gene="pdeC"
                     /locus_tag="OO468_001957"
                     /pseudo
     CDS             complement(26745..28322)
                     /gene="pdeC"
                     /locus_tag="OO468_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28751..29032
                     /locus_tag="OO468_001958"
     CDS             28751..29032
                     /locus_tag="OO468_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR6259086.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7284 a         7776 c         7352 g         6631 t
ORIGIN      
        1 ggcaaatatc aatctggcat ttgtcggaat tatttatacc gccctttttg ctctctacgc
       61 catgctgttc tggctggcat ttttctaatc tatttgagac gcgctcatgg aaagtacgcc
      121 gaaaaaagca cctcgcagta aattccctgc tctgttagtg gttgcgttgg cgctggttgc
      181 ccttgttttc gttatctggc gcgtagacag tgcgccatca actaatgacg cttacgcgtc
      241 agcagatacc attgatgtgg tgccggaagt cagcggtcgc attgtagaac tggcggtcac
      301 cgacaaccag gcggtgaaac agggcgattt gctgttccgc atcgacccgc gcccgtacga
      361 agccaacctg gcgaaagccg aagcctccct cgcggcgctg gataagcaaa ttatgctcac
      421 ccagcgtagc gttgacgccc aacagtttgg tgccgactcg gttaatgcca cggtagaaaa
      481 agcccgtgcc gccgcgaaac aggccacaga tacattacgc cgcaccgagc cattactgaa
      541 agagggtttt gtctcagcag aagacgttga ccgtgcaaga acggcgcagc gcgccgcaga
      601 agcggatctt aatgccgtat tgttacaggc gcagtcagcc gccagcgccg tcagcggcgt
      661 cgatgcgctg gtggcgcaac gtgcggcggt cgaagcggat attgccctga ccaaactaca
      721 tctggaaatg acgaccgttc gcgcgccatt tgatggccgg atcatttccc tcaaaacctc
      781 cgtcgggcaa tttgcttctg ccatgcgccc tatttttacc ctcatcgaca ctcgtcactg
      841 gtacgtgatc gccaacttcc gcgaaaccga tctgaaaaat attcgctcag gtacacccgc
      901 aacgattcgc ctgatgagtg acagcggcaa aaccttcgag ggtaaagtgg attcgattgg
      961 ctacggcgtg ctaccggatg acggcggcct ggtgctgggc ggcctgccaa aagtgtctcg
     1021 ttctattaac tgggtccgcg ttgcccagcg ttttccggtc aaaatcatgg tcgataaacc
     1081 tgacccggaa atgttccgca tcggcgcttc ggcagtcgct aatcttgagc cgcaataatg
     1141 agcgcgctca actccctgcc attaccggtg gtcaggctgc tggcgttctt tcatgaagag
     1201 ttaagcgagc ggcgacccgg ccgcgtgccg cagatcgtgc aactctgggt aggctgcctg
     1261 ctggtgattc tgatctcgat gacttttgag atcccttttg tggcgttatc gctggcagtg
     1321 ctgttttacg gtattcagtc gaacgcgttt tacaccaaat ttgtggcgat cttgtttgtg
     1381 gttgccacgg tgctggagat cggcagcctg tttttgatct acaaatggtc atacggcgaa
     1441 ccgttgatcc gattgatcat cgccgggccg atcctgatgg gctgcatgtt tttgatgcgc
     1501 acccatcggc tgggactggt ctttttcgct gtcgccattt acgggcaaac cttccccgcc
     1561 atgctcgact atccggaagt agtcgtgcgc ttaacgctgt ggtgtatcgt tgttggcctc
     1621 tatccaacct tgctgatgac gttaatcggc gtgctgtggt ttcccaatcg tgccattacg
     1681 caaatgcatc aagcgcttaa tgatcggctt gatgatgcca ttagccacct gacggacagc
     1741 ctcgcaccgc tacccgaaac gcggattgaa agagaggcgc tggcgctgca aaaactgaat
     1801 gtcttttgtc tcgcggacga tgccaactgg cgaactcaaa gcgcatggtg gcaaagctgc
     1861 gtggcaacgg taacctacat ttactcgacg ctgaatcgct acgatcccac ctcttttgct
     1921 gattctcagg caattattga atttcggcaa aaattagcct cagaaatcaa caagctgcag
     1981 catgccgttg ctgaaggtca gtgctggcaa agcgactggc ggctcagtga aagtgaagcg
     2041 gtggcggcac gggaatgtaa cctggagaat atctgccaga cgttgttaca actgggccag
     2101 atgaacccga atacgccgcc aacgcccgcc gccaaaccgc catcaatggt cgccgatgct
     2161 tttaccaatc cagactatat acgctacgcg gtaaaaacat tgctcgcctg tttgatctgt
     2221 tacacctttt acagcggcgt ggactgggaa ggcattcaca cctgtatgct gacctgcgtg
     2281 atcgtcgcta atccaaatgt cggttcgtcg taccagaaga tggtgctgcg ttttggcggg
     2341 gccttttgcg gcgcgattct ggcgctgtta ttcacgctac tggtcatgcc ctggctggac
     2401 aatattgtcg aattgctgtt tgtgctggca ccgattttcc tgttgggcgc atggattgcc
     2461 accagctctg aacgctcttc ttatatcggc acacagatgg tggtcacctt cgcgctcgcc
     2521 acgctcgaaa acgtttttgg cccagtgtac gacctggtgg aaattcgcga tcgcgccctg
     2581 ggtatcatca ttggtaccgt ggtgtccgcg atgatttaca cctttgtctg gcctgaaagt
     2641 gaagcgcgca cgctgccgca aaaactggct ggcgcgctgg gtatgttaag taaagtaatg
     2701 cggatcccac gccagcagga agtcacggct ctgcgcactt atctgcaaat tcgtatcggt
     2761 ctgcatgcgg cgtttaatgc ctgtgaagag atgtgccaac gcgtggcgct ggagcgtcaa
     2821 ctggacagcg aagaacgcgc cttactgatt gaacgttcgc aaacggttat tcgtcagggc
     2881 cgcgatatcc ttcacgcctg ggatgccacc tggaattcgg cgcaggcgct ggataacgca
     2941 ctacagccgg acagagcagg tcagtttgcc gacgccctgg agaaatacgc tgccggtctg
     3001 gcaaccgcac tcagccgttc tcctcaaata acgcttgaag agacacccac ctcgcaggcc
     3061 atcctgccca ccttattaaa acaggagcaa cacgtctgcc agcttttcgc ccgcttgcca
     3121 gactggacag ccccggcatt aacgcccgcc acggaacagg cacaaggagc cacgcaatga
     3181 tcaatcgtca actttcacgt ctgctgttgt gcagcattct cggcagcacg acgctgattt
     3241 ccggctgtgc cctggtacgt aaggattctg cgcctcatca acagctcaaa ccggaacaaa
     3301 tcaaactggc cgatgatatt catcttgcca gctccggctg gccgcaggcg cagtggtgga
     3361 aacaactcaa tgacccgcag ttggatgccc tgatccaacg gacgctaagt ggttcacaca
     3421 ccctcgccga agcgaaactg cgggaagaaa aagcgcaatc gcaggccgat ttgttagatg
     3481 ccggttcaca gttgcaggtg gcagcgttag ggatgcttaa ccgccaacgt gtctcggcga
     3541 acggcttttt aagcccttat gcgatggatg cgcccgcact ggggatggac gggccgtact
     3601 atacggaagc cacagtaggt ttgtttgccg gactggatct tgatttgtgg ggtgtgcatc
     3661 gctcagcggt tgccgccgcc attggcgcgc ataatgccgc gctggcagaa accgcagcag
     3721 tagagctatc gctgaccacg ggcgtagcgc agctttatta cagtatgcag gccagctatc
     3781 agatgctcga tctgttagaa caaactcgcg atgtgattga ttacgcggtg aaagcgcacc
     3841 aaagtaaagt ggcgcacggt ctggaagcac aagtgccttt ccacggcgcg cgggcacaga
     3901 ttctggcggt cgataaacaa attgctgccg tcaaagggca aatcaccgaa acgagagaat
     3961 ctctgcgcgc attgattggc gcgggagcca gcgatatgcc ggagatcaaa cctgtggcat
     4021 taccacgagt ccagaccggc attccggcga cactctctta tgagttgctc gccagacgcc
     4081 cggatctgca agccatgcgc tggtatgttc aggcgtcatt agatcaggtg gattccgcgc
     4141 gggcgttgtt ctatccgagc tttgatatca aagcgttttt cggccttgat gccatccacc
     4201 tggatacctt attcaaaaaa accagtcgcc agttcaactt catcccgggt ctgaaattgc
     4261 cgctgtttga cggtggacgg ttgaatgcca atctcgaagg cacgcgcgcc gccagcaaca
     4321 tgatgattga acgttacaac cagtcagtac tgaacgcggt gcgtgacgtt gccgtcaacg
     4381 gcacgcgtct gcaaacgctc aacgacgagc gagaaatgca ggctgaacac gtggaagcaa
     4441 cgcgcttcac ccagcgcgct gccgaggccg cctatcagcg cggcttaacc agccgcttac
     4501 aggccactga agcccggttg ccagtacttg ccgaagagat gtcattactg atgctggaca
     4561 gccgccgggt gatccaaagc attcagttga tgaaatcgct gggcggcggg tatcaggcgg
     4621 ctcccgtcgt cgagaaaaaa taaaatgtct gccgcgtgat ggctgtcacg cggtatttcg
     4681 tttcgtcacg tcaaaactga cgacagcctg tttttcgtca gagttttgaa taaatagtgc
     4741 ccgtaatatc agggaatgac cccaaataaa atgtggcata aaagatgcat actgtagtcg
     4801 agagcgcgta tgcgtgattt gattaactgg agcgagaccg atgaaaaaag tcgtcacggt
     4861 ttgcccctat tgcgcatcag gttgcaaaat caacctggtc gtcgataacg gcaaaatcgt
     4921 ccgggcggag gcagcgcagg ggaaaaccaa ccagggtacc ctgtgtctga agggttatta
     4981 tggctgggat ttcattaacg atacccagat cctgaccccg cgcctgaaaa cccccatgat
     5041 ccgtcgccag cgtggcggca aactcgaacc tgtttcctga gatgaggcac tgaattacgt
     5101 tgccgagcgc ctgagcgcca tcaaagagaa gtacggtccg gatgccatcc agacgaccgg
     5161 ctcctcgcgt ggtacgggta acgaaaccaa ctatgtaatg caaaaatttg cgcgcgccgt
     5221 tattggtacc aataacgttg actgctgcgc tcgtgtctga cacggcccat cggttgcagg
     5281 tctgcaccaa tcggtcggta atggcgcaat gagcaatgct attaacgaaa ttgataatac
     5341 cgatttagtg ttcgttttcg ggtacaaccc ggcggattcc cacccaatcg tggcgaatca
     5401 cgtaattaac gctaaacgta acggggcgaa aattatcgtc tgcgatccgc gcaaaattga
     5461 aaccgcgcgc attgctgaca tgcacattgc actgaaaaac ggctcgaaca tcgcgctgtt
     5521 gaatgcgatg ggccatgtca ttattgaaga aaatctgtac gacaaagcgt tcgtcgcttc
     5581 ccgtacagaa ggctttgaag agtatcgtaa aatcgttgaa ggctacacgc cggagtcggt
     5641 tgaagatatc accggtgtca gcgccagtga gattcgtcag gcggcacgga tgtatgccca
     5701 ggcgaaaagc gccgccatcc tgtggggcat gggtgtaacc cagttctacc agggcgtgga
     5761 aaccgtgcgt tctctgacca gcctcgcgat gctgaccagt aacctcggta agccgcatgc
     5821 gggtgtaaac ccggttcgtg gtcagaacaa cgtgcagggt gcctgcgata tgggcgcgct
     5881 gccggatacg tatccgggat accagtacgt gaaagatccg gctaaccgcg aaaaattcgc
     5941 caaagcctgg ggcgtggaaa gcctgcctgc tcataccggc tatcgcatca gcgagctgcc
     6001 gcaccgcgca gcgcatggcg aagtgcgtgc cgcgtacatt atgggcgaag atccgctgca
     6061 aactgatgcg gagctgtcgg cagtacgtaa agcctttgaa gatctggaac tggtcatcgt
     6121 tcaggacatc tttataacca aaaccgcgtc ggcggcggat gtcattttgc cgtcaacgtc
     6181 gtggggcgag catgaaggcg tgtttactgc ggctgaccgt ggcttccagc gtttcttcaa
     6241 agcagttgaa ccgaaatggg atctgaaaac ggactggcaa atcatcagtg aaatcgccac
     6301 ccgtatgggt tatccgatgc actacaacaa cacccaggag atctgggatg agttgcgtca
     6361 tctgtgcccg gatttctacg gtgcgactta cgagaaaatg ggcgaactgg gcttcattca
     6421 gtggccttgc cgcgatactt cagatgccga tcaggggact tcttatctgt ttaaagagaa
     6481 gtttgatacc ccgaacggtc tggcgcagtt cttcacctgc gactgggtag cgccaatcga
     6541 caaactcacc gacgagtacc cgatggtact gtcaacggtg cgtgaagttg gtcactactc
     6601 ttgccgttcg atgaccggta actgtgcggc actggcggcg ctggctgatg aacctggcta
     6661 cgcacaaatc aataccgaag acgccaaacg tctgggtatt gaagatgagg cattggtttg
     6721 agtgcactcg cgtaaaggca aaattatcac ccgtgcgcag gtcagcgatc gtccgaacaa
     6781 aggggcgatt tacatgacct accagtggtg gattggtgcc tgtaacgagc tggttaccga
     6841 aagcttaagc ccgattacga aaacgccgga atacaaatac tgcgccgtgc gcgtcgagcc
     6901 gatctccgat cagcgcgccg ccgagcagta cgtgattgac gagtacaaca agttgaaaac
     6961 tcgcctgcgc gaagcggcac tggcgtaata cagtcctttc tacagcctcc tttcggaggc
     7021 tgttttttta tccattcgaa ctctttatac tggttactcc ctacccaatc gtattatcaa
     7081 aatgaaaaaa attatcgcat tgatgttgtt tttgacattc tttgcccacg cgaacgactc
     7141 cgagcctggc agccagtatt taaaggcagc agaggccggg gaccgacgtg cacaatattt
     7201 tcttgccgac agctggttta gctccggcga tttgagcaaa gccgaatatt gggcacagaa
     7261 agccgccgac agcggtgatg ctgatgcctg cgcgctgttg gcgcagatca aaatcaccaa
     7321 tccggtcagt ctggactatc cacaagcaaa agttcttgca gagaaagcgg cgcaagcggg
     7381 cagtaaagaa ggtgaagtaa cgctggcgca tattctggta aatactcaag cgggtaaacc
     7441 ggattatcca aaggcgattt cgctgttaga aaacgcctcg gaagatctgg agaatgactc
     7501 cgccgtcgat gcccaaatgc tgcttggttt gatttacgcc aacggcgtgg gcattaaggc
     7561 cgacgatgac aaggcaacct ggtatttcaa acgcagctct gcaatttccc gaaccggtta
     7621 ttccgagtac tgggcgggaa tgatgttctt aaacggtgag gaaggtttta tcgagaagaa
     7681 caaacaaaag gcgctgcact ggttgaacct gagctgtatg gaagggtttg ataccgggtg
     7741 tgaagagttt gaaaaattaa ccaacggtta aggtaggcct gataagacgc ggcaagcgtc
     7801 gcatcaggca tccacacatt gccgggggat atccccggca atcttcaatt attgatccgc
     7861 agttttatca aatttgccca gcacttcacg ctcataagcc agcgcctttt tgcggtcaaa
     7921 tttgtgttcc cacttggcaa tcaccagcac cgccagcgca ttacccacca cgttcagcgc
     7981 agtacgcgcc atgtcgagga tacggtcaac accagcaata aacgccagac cttccagcgg
     8041 aatacctacg ctacccagcg ttgccagcaa caccacaaac gacacgccag ccacgccagc
     8101 aatccctttc gaggtcacca tcagcgtcag caccagaatg atttcctgcc agatggacag
     8161 gtcaatgcca tacagctgag cgatgaaaat agcggcaata ctttgataca gcgtcgaacc
     8221 atcaaggtta aaagagtaac cggtcggcac cacgaagctg gtgatcgacg ccggtgctcc
     8281 gtaggcttcc atcttctcaa taattcgcgg cagtacgctt tcagagctgg cagtggagta
     8341 cgccagaatc agctcatctt tcagaatacg aatcaggatc cagacgctta acccgcacag
     8401 acgtgccaca attcccagca ctaccagcgc gaagaacaga atggcgaaat gcaccagcag
     8461 caccagtttc gccagtggcc acagagacga gaaaccaaag ttagccaccg tcaccgcaat
     8521 cagcgcaaac acacccaccg gtgcataacg catcaccatg tgagtcactt taaacatggt
     8581 ttcagagatg gagcggaaca cggtcaccag cggttcacga tgcgtcgcgg gcagggaaga
     8641 aagccccaga ccaaacagca ccgagaaaaa gatgatcggc agcatttcgc ctttcgccat
     8701 cgacgccaca atgttcgtcg gcaccagcga caaaatcgtg cccataatgc cgtgggaact
     8761 gctttgtacc gcttccgtag tgctctgata tttcgagata tcgacggtcg ccagctgcga
     8821 catatccacc ccggcaccgg gctggaagac gttcgccaga gtgatcccca aaatgatggc
     8881 gacggtggtg atcacctcga agtaaataat ggttttcgcg ccaatacgcc cgagctgttt
     8941 ggcatcacca acacccgcga tacccaccac cagcgtggag atcacaatcg gcacaacaat
     9001 cattttgatc agatggatga agatatcacc cgccggagag agcaaattga cgaccagcca
     9061 gtcgcggctg tcgctatggt agtgcaggta gcttcccagg agaatgccca gcaccatagc
     9121 aaacagaatc tgccaggcca ggctgaattt tatatttttc atcagaataa cttcctcaat
     9181 ggagcgccgt tccccgttca gaaacgcgag aacgtgacat actgccaggg catgaattgt
     9241 gttgcgttgg tttgtagatt tttttaacgc ttatgatcag tatcatttgc gcggtatgtt
     9301 gcgcaaccct ttaacaacag agcttttcgc tgcattaatg cataataacg cgagtttatg
     9361 gcaggattaa tgaataacca tttgttatgg aaagattttt atttgtttaa tctgcaataa
     9421 tactgattgg tgattatttt tggcatatgt tcattcgatt attttttatg cagctcaatg
     9481 acataatctt catcaccaga tgattaatcc gatctccgct gcaacagttt tgccatatta
     9541 atcgactcga ccagctgtgt ccggttgatc cggggtgagt tgagatccat cactttttgc
     9601 catagttcga tggcttgcgc gtaattcgcc tgcataaacg catcggaagc cagcagcatc
     9661 agagcggtga tttcgttaga gtccagcgcg agggctttgt cgatcattgc gcgagtctgg
     9721 gcggtcatat gctggctggc ctggtaataa agcaccgtcg ccagcgccgc atacagttca
     9781 gcgttctcac cacgtagttg caacgcctga cgatacgcca gcagcgaatt gctgtaatcg
     9841 ttttgccaca gatagtactc gcccagtaac gcccattgtt cgctgttttg tggattagcg
     9901 cggattttat cctgcaatgc ctgaagctgc gcttccgggg tttgctggct ggcaaactga
     9961 tgtagcggat cgcgctgacg ctgatactcc gcacgtaccg cctgccattt tgggcttaac
    10021 agataactgc cgacacagag gaataccatc aatatcgtga gtgttgtcag cattttcacc
    10081 ggtatttgag gctgtttcat cgcttagccc tcactcgcca gaggatcagt gccatcagca
    10141 ataacaacac cactggcagc gcccacaaca ccagcgtctg acccgttaac ggtgggttat
    10201 agcgaacaaa atctccgtag cgttcggtca tccagccaat gatttcgact tcgttcttcc
    10261 cctccgacac catgctataa acctgatggc gcatactgac cgccaccggc gcattggatt
    10321 cgagtaagtt ttgattctgg cactgcggac aacgtaactg gctggcaata tttaacgcct
    10381 gttgctgttg ttgcggattg gcgaattgcc aggtgtctac aacctgagcg cgcgcaaaac
    10441 aggtaaataa cagcagtagc gtgagaagcc ctttattcat cgcgcttctt cccccgccat
    10501 ccgcttaaca atgcgcccgc aatcatcaac aatcctcccc cccagatcca gcgcacaccg
    10561 ctttgtacat acaaacgaaa agcgtaacga tccgctctgg ttttttcacc catgaccgca
    10621 taccagtcat ggatgccgtt ccagcgaatt gacggttcca tcatttgctg acggcgtgct
    10681 tcataaaaac gccgctccgg cgttaattca ccaatgcgtt gctgatggtc aaacagcgcc
    10741 actatcgctt tttcgctggt gtaattgcct ctggcttgca gatcgagtcg ctcaaaacgg
    10801 aaggtgtatc ctgccagcgt cacctgctga cccggctgta aattcaggct gatctcctga
    10861 cgactgacac tggaaaccac gatccccgcg gcaaataaca gcacgccagc atgagctacc
    10921 agcgccggaa gctgcacgcg tttgccagag acaaacgtcg ccagcacaat caccaccagc
    10981 atcaacagac caaacggtaa cgtcgcgcgg ttaaaatacg gcgcgccaac ggagaggcgt
    11041 ccccagccca gcaggccgta aatcatcgga taaagcgttc ccaccagcac gatcagtagc
    11101 actgcgcaaa acagcaacag cgtagcgagg attaacattt cccgcgataa ccccgaaaaa
    11161 cgcaccgccg ggccaccgtc ccgcgctcgc cagccataca gagccaaaga cgcaaggcta
    11221 atcagtgcaa acaggctgaa caacggcacg gcgcggacgt tatccagtgc gaacgcatga
    11281 accgaaacca gaatgccaga acggacaatt aaggtgccca gcagcgacag catcagagta
    11341 actatcgcca gcaacagcga ccagtggcgg aaaatccccc gctggcgtgt cagggacaaa
    11401 ctgtgcagca gcgcagtggc agaaagccag ggtaataaag aggcgttttc caccggatcc
    11461 cagaaccacc agccgcccca gccgagttcg cagtaggccc accaggaacc gaggatgatc
    11521 cccgccgtta atgcactcca gccaggtaac gcccagcgcc agcaaattcg ggcgctggcc
    11581 gcatcaaact cgccgcgcag tagactcgcc agcgccacgc tcgccgctac catcaaaccg
    11641 ccatagccaa ggtaaagcag cggtggatga aagataagac cgggatgttg cagcatcgga
    11701 ttgagatcgc ggccttcgat tgctggtgga aatatccgca cgaagggatc ggaccacagc
    11761 accacaaaca gtagcagtgc ggcgagcatg agagataaaa cggctagcgt cagcggaaat
    11821 agcggatcgc tttgctgccg ataatgccag gcaaacagcg cgctccaggc ggaaagcagc
    11881 agcacccaaa gcagcagcga accttcatga ccgccccaca ccgccgccag tttgagttcc
    11941 cacgacagca ggctgtagct atgttgggcg acataaatga ccgagaaatc gctgatgaga
    12001 aaacaatacg tcagtacgcc aaaggcgagc agcaggagcg cgaactgcgc cagaatgccg
    12061 atgcaagtga gtcgcatcat ggcaggccag cgcaaccgca ctcccgcaaa ggccgtcaac
    12121 ggggtcaaca cgttgacccc gagacttaac aacaacgcca gaaaaccgac ttcaggaagg
    12181 aatacgtcca gaaaccacct cttttctggc ttacgccacc gtcaactgtc cggcgtagag
    12241 aatgaaaaag cgcagcatca acacgccgac cagactggcc ccacacgccg ccagcacggc
    12301 aggaatgccg gaactgcgat tgacccacgg tttgagcaac attggcacaa tcagccccag
    12361 cccggcgaca ccaagccaga accaccacgt ccagaaaccg ccacctaatg ccgccaccag
    12421 cgcacgaact ttaccgtcat cgcccagcgc cagaccgaca aaaaacgcca ccagcaggaa
    12481 gatttcaccc cataccacgg ggatttccat ccggtgaaca aagtgcgctt ccgtggaatg
    12541 cgggttactg cgttggcgta tcgccatggc gatcagcgcc accgccgcac cggacgagat
    12601 gccggagaag aggagcagca ccggcaggct cgggttattg aggaacggat acgatttcag
    12661 cgccgacagc agaaagccgg tataagcccc cagcagcacc gccaacacca gcatcagcgt
    12721 ttccagtccg cgatgaacgg gcgtcagcag gctcagaacc ttttgcacga tccccagctt
    12781 cggcaaccag cgttgttgca gggcaagcaa atcatgttca aagattttcg ccagccacag
    12841 caccagcacc accatgtaga gctgaaacag catcaccccc atcgacatca ccgaggtaaa
    12901 actgtagtgg aacatcagct tccagaaggt ccacggtctt gtcaggtgga agacgaggat
    12961 caacagaccg aggatcaccg cgcccggccc gacaatcagc gtggtgcgca gcaacgtact
    13021 gtctgcaccg cccgcctgcg ggtagaagcg acgtaacagc acggccagcg tcaccagacc
    13081 ggcagaaata ccaatcaaaa acaggtagat ggcaatcggc cagtcccaca ccagcgattc
    13141 aaaatgaaat gcggaagtct gcgtcattgg ctcacctccc cgtatttaaa cggtacgcgg
    13201 tacagcttcg gtttggttcc cagcgccagc ttgtagcggt aagtaggctt ctggcgcagc
    13261 agttgcgaaa tctcactgtt aggatcgtcc agattgccaa acgtcagcgc cttcgtcggg
    13321 caagcttcaa cgcacgcggg aagcttaccg gcctgcaaat tggtcttacg acagaaatcg
    13381 catttgtccg ccgtcttcgt gaccggatgg ataaagcgca cgcggtacgg acaggcggcg
    13441 atgcagtact gacaaccgac gcagagatca ggattaacat cgacaatccc gctagcggca
    13501 tcacgaaaag acgcaccggt cgggcagacg tcaacgcacg gcgcatgatc gcagtgctgg
    13561 caagacttac ggaagaagcg atatttcaca tcaggaaatt cgccctgcgg ctcgctacga
    13621 ataatcgtca ggcgcgagac gccttccggc accttgttca cttcccgaca agcatccata
    13681 caggcggtac agccgatgca taacgactcg tcatgcacca tgccgtaacg caccccattg
    13741 atattcaacg ttttcgccac cacgcgccct gcggtgccgc tgacggctgc cagcacgccg
    13801 acgccggtga gaaactggcg acgagaccag gtcatggctg ctccttaagc aacggaacag
    13861 acgccgggtt aaagttcgga ttggtgcgct gatcgctgtg gcaatcgacg caaatcttaa
    13921 tccgtccttt gtcacttaac gtctgcatcg tatcttgctg tggatgcagg gaatggcagc
    13981 tggcgcacgc cactttggtt acgtggacat cgtgcggcca gaacgctttt tgcaactgtt
    14041 ccggcagatg acaggacata cagacgctgt tctgctctcc gaccttatac atcggctcgt
    14101 taaagcgcat cacatctttc accccttcgc ggtgttgcgg tgatgcttgg ccgtgacagt
    14161 tggtgcaggt gaccggcaat ttgttattcg ggttgataac ggaagcatgt ttgccatgca
    14221 taccttcggt atctggttta tgacagtcca gacaggcggc atccgggtta cgctgctggg
    14281 taacttcgta gcgatcacca gatgcctgca ccgcaggggt agcggtaatt ccgttcaggc
    14341 tccacaacag gcccgacgcc agcaccccag cagttaataa cgaacgtaat acgctcatat
    14401 tcactccatt gtgttttccg ccccttgcga ggcggaacgg ggttattggc ttaacagacc
    14461 gtttttacgt gcctgctctt cccactgcgg gatcaccgtt ttgatgaagt cctgcttctc
    14521 ggccttgatt tgttccatgt tcaggccaat ggcctgctgg gctttctctt tggtcgagat
    14581 atccgggatc tggatttcat gggtgatacc tttggtcgcc agcaggcgcg ccagtttggt
    14641 gcgcgcatcc gccgctttat ccatcgccgt accgagcatc cgtagacctt cttccggtgc
    14701 gtgcatatga ataccgtggg aagcaatcgc cagatcccaa cgccactggg catgacggat
    14761 atcgtcctga atcggcttca tttcggcttc cgtcgcgcct gcatccagcg ccgctttcgc
    14821 ttcgaagtga gcgtgaacca gttgatcttc aaccttgatt ttcaggtcgt taatcgattg
    14881 cttacgttcc gcgaccactt tttgcaaggc agctttatcc tgggtatggc agttcgcaca
    14941 agtctgggcg aagttatcaa acggattacc aattttatgg tcggtgtaga gtttgccttc
    15001 ggcgttctgc acttttggca tatggcagtc gatacaggtc acgttgtttt taccgtgaat
    15061 gcccgctgtc caggtttcat attccgggtg ctgcgctttc agcattggcg ttttcgacag
    15121 ggagttagtc cagtcagaga aggcaatttt gtcgtaatac tgctccatat tttcgacttt
    15181 catgccgtca tcccacggga atttaaccgc tttgtttttg ccgtcgaagt aatactccac
    15241 atggcactga ccgcaaacca tcgattgctg gtcgaaacgt ccggctttct caaaaggttt
    15301 accaatggct tccatcgcgc gagccgcata cggacgggaa agggttaact ccggtttgcc
    15361 tttggcgaac tctggtgagg cggtgttatg gcagtcggca caacctaagt tgttgacgat
    15421 ttccgggccg ccgcgcgccc atttaccgtg gaagtagcca tcttcgccgt ctttctggat
    15481 cagacgcgcc acatccgggc ttttacaact ccagcacgcc atcggtagcg gaccatcttc
    15541 agcgtttttc ggcgcaccgg tacgcagggt ttcacgcaca tcggtcacag caaaggcatg
    15601 tccacgaggc ttgttgtaat cgcgcgagaa gggatacccc gcccacagga tcaccagccg
    15661 tggatcttcc gccagggcgt caacacgctc tgactgttcc gaggtggctt tccaggagag
    15721 atattgatcg tgatgctgcg gggcaaaggt ttcattcttc gcttccacag ttacaggttt
    15781 tgcgggagct gccgtttgtt cagcgtgaac agaagtgaaa aagaaaaaag gaatcaataa
    15841 gctgaagata cggcgtgcgt ttatttttat ccttgtcata ggggcttcat ccgaattgcg
    15901 ccattgttgc aatggcggtt tttattgttt ttcacgacag taaccgcacc tacactgtca
    15961 tgacattgct cacccctatg tgtaacaaat aaccacactg tgaatgttgt ctttaatcaa
    16021 ttgtaagtgc atgtgaaata ccactttaga gttagtcagt atcttcctct ttttcaacag
    16081 catgcataac tgcatgttcc tcaaagaatt aatcaacttt tgttggtgac cttcaaaaat
    16141 taccctgccg tttatttgca ccattctact tttgcgtgat ctgtcgccca aatactaaac
    16201 aaaactgcca atacccctac atttaacgct tatgccacat attattaaca tcctacaagg
    16261 agaacaaaag catgagccaa attcacaaac acaccattcc tgccaacatc gcagaccgtt
    16321 gcctgataaa ccctcagcag tacgaggcga tgtatcaaca atctattaac gtacctgata
    16381 ccttctgggg cgaacaggga aaaattctcg actggatcaa accgtaccag aaggtgaaaa
    16441 atacctcctt tgcccccggt aatgtgtcca ttaaatggta cgaggacggc acgctgaatc
    16501 tggcggcaaa ctgccttgac cgccatctgc aagaaaacgg cgatcgcacc gccatcatct
    16561 gggaaggcga cgacgccagc cagagcaaac atatcagcta taaagagctg caccgcgacg
    16621 tctgccgctt cgccaatacc ctgctcgagc tgggcattaa aaaaggtgat gtggtggcga
    16681 tttatatgcc gatggtgccg gaagccgcgg ttgcgatgct ggcctgcgcc cgcattggcg
    16741 cgatgcattc ggtgattttc ggcggcttct cgccggaagc cgttgccggg cgcattattg
    16801 attccaactc acgactggtg atcacttccg acgaaggcgt gcgcgccggg cgtagtattc
    16861 cgctgaagaa aaacgttgat gacgcactaa aaaacccgaa cgtcaccagc gtagagcatg
    16921 tggtggtact gaagcgtact ggcggaaaca tcgactggca ggaagggcgc gacctgtggt
    16981 ggcacgacct ggttgagcaa gccagcgatc agcactaggc ggaagagatg aacgccgaag
    17041 atccgctgtt tattctctat acctccggtt ctaccggaaa accaaaaggc gtactgcaca
    17101 ctaccggcgg ttatctggtg tacgcggcgc tgacctttaa atatgtcttt gattatcatc
    17161 cgggtgatat ctactggtgc accgccgatg tgggctgggt taccggacac agttatttgc
    17221 tgtacggccc gctggcctgc ggcgcaacca cgctgatgtt tgaaggcgta ccgaactggc
    17281 cgacgcctgc ccgtatggcg caggtggtgg acaagcatca ggtcaatatt ctctataccg
    17341 cacccacggc gatccgcgcg ctgatggcgg aaggcgataa agcgattgaa ggcaccgacc
    17401 gttcgtcgct acgcattctc ggttccgtgg gcgagccaat taacccggaa gcgtgggagt
    17461 ggtactggaa aaaaatcggc aacgagaaat gtccggtggt cgatacctgg tggcagaccg
    17521 aaaccggcgg tttcatgatc accccgctgc ctggcgctac cgagctgaaa gccggttcgg
    17581 caacacgtcc gttcttcggc gtgcaaccgg cgctggtcga taacgaaggt aacccgctgg
    17641 aaggggctac cgaaggtagc ctggtgatca ccgactcctg gcctggtcag gcgcgtacgc
    17701 tgtttggcga tcacgaacgt tttgagcaga cctatttttc caccttcaaa aatatgtatt
    17761 tcagcggcga cggcgcgcgt cgcgatgaag atggctatta ctggatcacc gggcgtgtgg
    17821 acgacgtgct gaacgtctcc ggtcaccgtc tggggacggc agagatagag tcggcgctgg
    17881 tggcgcatcc gaagattgcc gaagccgccg tagtaggtat tccgcacaat attaaaggtc
    17941 aggcgatcta cgcctatgtc acgcttaatc acggggagga accgtcacca gaactgtacg
    18001 cagaagtccg caactgggtg cgtaaagaga ttggcccgct ggcgacgcca gacgtgctgc
    18061 actggaccga ctccctgcct aaaacccgct ccggcaaaat tatgcgccgt attctgcgca
    18121 aaattgcggc gggcgatacc agcaacctgg gcgatacctc gacgcttgcc gatcctggcg
    18181 tggtcgagaa gctgcttgaa gagaagcagg ctatcgcgat gccatcgtaa cccacaattg
    18241 ccggatgcga atcggtaacg gtttgtaggc ctgataagac gcgacagcgt cgcatcaggc
    18301 attgattgcc ggatgcggcg taaacgcctt atccggccta cattcggcaa gggttacccg
    18361 agcgttaacc ttctcccata agggagcggg aattaaaaca atccctacat tacctctgga
    18421 gaatctgtga tgaatggtac gatttatcag cggatagaag acaatgcgca tttcagggag
    18481 ttagtcgaaa aacggcaacg gtttgccacc atcctgtcga ttattatgct ggcagtttat
    18541 atcggcttta ttttactgat cgccttcgcg cccggctggc tgggcacgcc gctgaatccg
    18601 aacaccagcg tcacacgcgg tattccaatt ggtgttggag tgattgtgat ctcctttgtt
    18661 ctcaccggta tctacatctg gcgggcgaac ggcgaattcg accgtcttaa taatgaagtc
    18721 ctgcatgagg tacaagcatc atgaaaagag ttctgacggc gcttgccgcc acactccctt
    18781 tcgcagctaa cgccgcggat gctattagcg gggccgtaga gcgccagcca acgaactggc
    18841 aggcgattat tatgttcctg attttcgtcg tgtttacgct cggcattacc tactgggcat
    18901 caaaacgcgt acgttctcgt agcgactact acaccgcagg cggcaatatc actggcttcc
    18961 agaacgggct ggcgattgcc ggggactata tgtccgccgc ctcattcttg gggatctccg
    19021 cgctggtgtt tacctccggc tatgacgggc tgatctactc gttgggcttc ctggtgggct
    19081 ggccaatcat tctgtttctg attgccgaac gtctgcgtaa cctgggacgc tacacctctg
    19141 ccgatgtggc ctcttatcgt ctgaaacaag ggccgattcg tattctttcg gcctgtggtt
    19201 ctctggtagt ggtggcgctt taccttatcg ctcagatggt aggcgcaggt aaactgatcg
    19261 agctgctgtt tggccttaac tatcacattg cggtggtgct ggtcggcgtg ctgatgatga
    19321 tgtacgtcct gttcggcggc atgctggcga ccacctgggt acaaattatc aaagctgtgc
    19381 tgttgctgtt cggtgccagc tttatggcct ttatggtgat gaaacacgtc ggctttagct
    19441 tcaacaatct gttcagcgaa gcgatggcgg tacacccgaa aggtgtcgac atcatgaaac
    19501 cgggcggact ggtgaaagat ccgatctccg cgctctctct ggggctggga ctgatgtttg
    19561 gtacggcggg cttgccgcac attctgatgc gcttctttac agtcagcgat gcccgcgaag
    19621 cacgtaagag cgtgttctac gccaccggat ttatgggcta cttctatatt ctgaccttta
    19681 ttatcggctt cggcgcgatc atgcttgttg gtgcgaatcc ggaatataaa gacgcggcgg
    19741 gccatctgat tggtggtaac aacatggcgg ccgttcacct ggcgaatgca gtgggcggca
    19801 acctattcct cggttttatt tcagcggttg ctttcgccac tatcctcgcg gtggttgcgg
    19861 atctgacgct ggcgggcgca tcggcggttt cgcatgactt gtacgctaac gtcttcaaaa
    19921 aaggcgcgac cgaacgtgaa gagctgcggg tatcaaaaat caccgtactg atcctcggcg
    19981 tgattgcgat tatcctcggc gtgctgtttg agaatcagaa catcgccttt atggtaggcc
    20041 ttgcgtttgc catcgcggcg agctgtaact tcccgatcat tctgctttcc atgtactggt
    20101 cgaaactgac tacccgtggc gcgatgctgg gcggctggct ggggctgatt accgcagtgg
    20161 tgctgatgat cctcggcccg actatttggg tacagatcct cggtcacgaa aaagccatct
    20221 tcccgtatga atacccggcg ctgttctcta tcagcgtggc attcctcggc atctggttct
    20281 tctcggcaac cgataactca gcggaagggg cgcgcgagcg tgaactgttc cgcgcgcagt
    20341 ttatccgctc ccagaccggc tttggcgttg agcaaggccg cgcacattaa tctctcccct
    20401 tccccggtcg cctgaccggg gaatacgctt cctctccaac atgcatcacc ttttcccata
    20461 atattaaaca aataaactca ttaaaaaatg agcgattttt gacagtcgta gaaaatgata
    20521 atgcagagaa tatgcctttt ctttcttgtt aattataagg atattttatg cgctataatg
    20581 gtttaaataa tatgtttttc cctctttgcc agattaacga taaccactct atcacaagtt
    20641 catcacatac aaagaaaaca aaatcagata attacagcaa acatcataaa aacacgttaa
    20701 ttgacaataa agccctctct cttttcaaaa tagatgatca tgaaaaagtg ataggcttga
    20761 ttcagaaaat gaaaagaatt tatgatagtt taccatcagg aaaaatcacg aaagaaacgg
    20821 acaggaaaat acataaatat tttatagata tagcgttata tgcaaataat aaatgtgacg
    20881 atagaattac gagaagagtt taccttagta aagaaaagga tgtatccatt aaggtggtat
    20941 attttataaa taatgtcgcc gtccataata atactatcga aattccacag atagtaaatg
    21001 gtggttacga tttttcacac cttagcctga agggtatcgt gattaaagat gaagatttat
    21061 ccaattcgaa ttttgcaggt tgcagactac aaaacgctat tttccaggac tgtaatatgt
    21121 ataaaacgaa tttttattac gccataatgg aaaaaatact ttttgataat tgtattctcg
    21181 atgactcaaa tttcgctcag ataaaaatgg ccgacggaac tctaaatgca tgctccgcta
    21241 tgcatgttca attctacaat gcagcaatga atagagccaa tattaaaaat accttccttg
    21301 actattcaaa tttttatatg gcgtacatgg ctgaggtaaa tctttataaa gtaatagcgc
    21361 catatgttaa tttatttaaa gccgacctta gtttctctaa actcgattta attaactttg
    21421 aacatgctga tctgtctcgc gtcaatctga acaaagcaat cctccagaat ataaacttaa
    21481 ttgatagcaa actcttttgt acgtggctga caaatacatt cctcgaaatg gttatatgta
    21541 ccggctctaa tatggctaat gttaatttta ataatgccaa tttaagcaac tgccatttca
    21601 actgttctat tttaacaaaa gcctgtatgt ttaatacccg tctctatcgg gttaatttcg
    21661 atgaggctag cgtccaggga atgggcattt ccattctccg tggggaggaa aatatcccca
    21721 ttgatagtga taccctggta acactacaga aattctttga agaagattgt acctctcata
    21781 ctggcatgtc acaaactgag gataatatta atgcagtcgc tatgaagatt actgcagata
    21841 ttatgcaaca cgcagattga ggttaagtcg aagaacagcc aatgaattga ctttacaaga
    21901 gagataactt atttgtttta gaatcatgaa tgttcgcccg agctaacctg ttaaaggtgc
    21961 aaaagattgc acctttttca gattacctcg ggcctggtta cgtcgctttc ggcttactga
    22021 ttttcctcaa ttagcaacgc ttcaagcaaa tcgagatcgt gcagtaattt ttgcagcgtt
    22081 tcgttgctga tctcccgcgt ggcgcgcagg tggtacagtt cagcacgttc agaacgcaat
    22141 gccgccagac ggaagcgacg ctcaaggttc tcttcctgca cggaactttc aacgtcgtta
    22201 cgtccatcgg cgcgacgacg caggttacca atgacgcgag aactgacctc cgtaagcaac
    22261 tggttatcga tattctcttc ggtatccgcc gccagacgct cctccatttt ctggatcgcc
    22321 acaatcgcca cttctgccgt tgccgctcgc gcaatacgtt cttctttcaa ttgttgcgaa
    22381 tggtccgcga cttcaatgtg ttgtagcaga ataggcaaca tcaccacgcc gacaaacagc
    22441 gagaagagaa tgacgccagc cgccaggaac accagctcat agcgcgccgg gaagacgtta
    22501 ccatccggca agagcagcgg gattgagagc acaccggcca gagtgatcgc cccacgcacc
    22561 ccagcgaaag acgcgatcag gatttctcgt gtggtccacg aaccaaactc catcggcttc
    22621 ttcttcagga agcggttgct gaactttttc atcgtccaca gccagccgaa acggaccagc
    22681 atcagcgccg catatatcag aataatatcg gtaaacagca tccagatttc gacgttaggg
    22741 tcgatttctg ccgccatcag cgacgtctcc agaatacccg gcagttgcag acctaacagc
    22801 aggaacacca tgccgttaaa gacaaattcc agcatcgccc aggtgctgtt tgcgcgcagg
    22861 cgcattgcca gtggcgcacg gcgcatcaca ccggagcggg tgatggtcat cccggcggca
    22921 accgcagcga ggatgcccga aacgccaata tgttcggcaa tcagataaga agcgaatggc
    22981 agcaagaaca gcagaacgat ctgcgtcgcg ggttcatcac cgccccagcg gctgaggaat
    23041 cgcagcgagc gaccatacag ccagctcacc acaaaaccgg caagaatacc gccaatggct
    23101 actttcataa attcgaccgt cgcaccgccg acagtgaaga tcatcgtccc catcgccact
    23161 gccacggcaa acttcaacga caccaggccg gaagcgtcat tcatcaacgc ttcgccctgc
    23221 aaaatgccca tgattttttt cgggatgcgc ccttcgccaa caatcccgga gagcgccaca
    23281 gcatcggtcg gagaaagtac cgccgccagc gcaaaggcgg ggatcagcgg aatgcccggc
    23341 accacccagt aaataaggaa gccaatgccg accacggtga ccaccaccag cgccagcgcg
    23401 aggccgaaaa tctctcgacc atgttcgaga aattcacggg tcggcgtttt ccagccatca
    23461 gcgaacagca acggcgggat aaacaagact aaaaagagtt caggatcaaa ctccacatgc
    23521 aaaccaaacg tcggccacgc cagtagcgca ccgatggcga tttgcataag cggaagcggg
    23581 atctgaaagg gcatgacacg agtgaccacc ccggacagcg agaccacgag ggtcattatc
    23641 agtatggtga agaagatttc catgcgttcc ctgtttgcga tgttttttat gatctgagaa
    23701 agacgttatg cctgaattct cattttttca gaatatcgca aaagctcaca caatgtgcgg
    23761 agaaaataaa aacgggcagc cattgccacc cgttttcaga atccgatgaa agattagata
    23821 gcccagccac ccgcatagaa ggtcaccagc gccacggcga taacgaccgt accgatgttc
    23881 aacttgcgcc attcaccgga aaccagacga ccaatcacca gagtcgcgaa gccgatcatg
    23941 atgcctgtta cgatgttaca ggtcagcacg atgaataccg ccgtaaccag acccgccatc
    24001 gcatcaacaa aatcagcaaa gtcgattttc gccacgttgc tcagcatcag caggccaacg
    24061 tacatcagcg ccggagccgt tgcatacccc ggaacgaggt aagaaagcgg agagaggaac
    24121 agaatcagca ggaacagcac gccaacggtg atagccgtca acccggtttt accgcccgcc
    24181 gccgtacccg ccgcagactc gatgtatacc gctgccggag ctgcacccac caggccagag
    24241 aaaacgctgc tcatggagtc agtggtcagt gctttcccgc cgtcgatgat ctgcccatct
    24301 ttatccagca ggttcgcctg gccggcgaca gcgcggatag ttccggtggc atcaaatact
    24361 gccgtcatca ccagcgccag aacgcttggc aggactacag gattcagcgc gcccataatg
    24421 tccaggctgc caatcagtga attgccgttt tcatcgctca atgaaggcat ggcgaaaacg
    24481 ccggagaaat ggacgttagg atcgaagatc aaaccgacaa ttgagatacc gataatggtc
    24541 agcagaatgc caccagggac tttcagtttt tccaagccga tgatcaccgc cagacctgcc
    24601 agtgacataa tcaccgggaa ggtcgcgaaa tcacccagcg caacgggcag accatcaagc
    24661 gggtttttaa tcaccagacc gacaccatta gcggcaatga gcagcaggaa cagaccgata
    24721 ccgatccccg tgccgtgcgc cacaccgtgc ggcaagttgc gcaaaatcca gctacggata
    24781 cccgtggcag aaattaccgt aaacagcaca cccatcagga acacggcacc cagcgcgaca
    24841 ggtacgctaa tatgttgccc cagcaccagg ctgaatgcgg taaacgccgt cagggagatg
    24901 gcgcaaccaa tcgccaacgg cagattagcc cacagaccca tcacgataga accgagtccg
    24961 gcaaccagac aggttgcaac gaaaactgcc gcaggcggga agcctgcttt acccaacata
    25021 cctggaacga cgatgaccga gtagaccatc gccagaaacg ttgttaaccc ggcaaccact
    25081 tcctgacgga cagtgcttcc acgttgtgaa attttaaacc aggcgtcgag tgaaccgccg
    25141 gtacgcgctg atggcgtaga cataagaaaa catcccctga gagtttaatt ttcgtcaaga
    25201 tgcgtggcgg acaatcccct gccagcgaaa cgttatcttc ctgctccagg tttgcggcaa
    25261 tttggtgcgt cgcaaaaaag caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa
    25321 aaagcaaacg attatccggc gatcatagcg gctttttcaa ccgaagtttg ctgctttttt
    25381 ctcttttatt gtcttgacga tgaaaaacca ggcaattgat gcgcaaacgt tatcgtcgat
    25441 gtgcaagatt gcagcgttat cgagccgtat accgttctgg attgcgataa aaaatcgtct
    25501 gagatgacaa aaatattgcg gctaaaaagt atacttttac actggcgaag aaacgtcggt
    25561 gacatacaat taaagcatca acaccaaccg gaacctccac cacgtgctcg aatgaggtgt
    25621 gttgacgtcg ggggaaaccc tcctgtgttc cagcgggata gagagaaaga caaagaccgg
    25681 aaaacaaact aaagcgccct tgtggcgctt tagttttgtg tttgcggata attccaaaag
    25741 agctcgatca gtcccctcgc cccttcgggg agagggttag ggtgagggga acaggcagca
    25801 ccagtgcaaa tatttaccct caccccggcc ccatagttaa cgatgagtca gcttgtcctt
    25861 gtggcgcttt agttttgttc atcttccagc aagcgtgcgc cggtaccttc ttctcctaag
    25921 cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa ggcagccaca accaatacat
    25981 ccgtccagtt cgtcacgcag cgccactaag gtatgaatgc gccgatccaa ctcttctcgc
    26041 cattgggacg aaagctgttt ccactctttc gcacttaacg tatgcccttc tggcaacacg
    26101 ccaaacgctt caccaatggt cgccagcgga atgccaatac gctgagcgat tttgataatt
    26161 gcgacatatc gcaacacatc acgtttatat cgccgctgat tgccgctgtt acggatacta
    26221 gtaatcaacc ctttgctttc atagaaatgc agcgccgata ccgccacacc gctgcgtttc
    26281 gccacttcgc caggggttag cagcgcttta atgcggggta atttcttttc cataaatcgc
    26341 tttacctcaa gttaacttga ggaattatac tccccaacag atgaattaac gaactgaaca
    26401 ctgaaaagag gcagatttat gtcccatcag aaaattattc aggatcttat cgcatggatt
    26461 gacgtgcata ttgaccagcc gcttaacatt gatgtcgtcg caaaaaaatc aggctattca
    26521 aagtggtact tgcaacggat gttccgcacg gtgacgcatc agacgcttgg cgattacatt
    26581 cgccaacgcc gcctgttact ggccgccgtt gagttgcgca ccaccgagcg tccgattttt
    26641 gatatcgcaa tggacctggg ttatgtctcg cagcagacct tctcccgcgt tttccgtcgg
    26701 cagtttgatc gcactcccag cgattatcgc caccgcctgt agttttattg cccgcgcgtt
    26761 aactcccgcg cgggtaattg ctccatccat tgcataaaca cctgcggcgg catcgccttc
    26821 gcaaaaaacc atccctggca atagcgcacg ccgcgtttgc gcagccagtt aacctgctcc
    26881 tcagtttcga cgccttcagc gatcgttttt aaccccaggc tgtgcgccag ctcgatgatg
    26941 tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg tttcaacaaa cgatttatcg
    27001 attttcaaaa tatcgacatt caatgattta aggttatgca agttagagta gccaatacca
    27061 aaatcatcaa ttgccacttc gtaaccagcc tggcggaaag ccagaattaa tcggcgtcat
    27121 tttttcgaca tcaagaaatg catgctcagt cacttcaaat ttaatttgct gcggacgcac
    27181 cgcgtattgc tctgttttct gattgattcg cgctatcaac cgtgacgtat gaaaatcgga
    27241 ggccgacagg ttaatagaaa catagcgatc ggcatgtgtt gccaggtacg cgcccagatc
    27301 gcggaagaca ttatcaataa cataatcagt tatctgttct atcatcccct ccttttctgc
    27361 cagcggaata aactctgccg gattcattat ttgcccctgc tcaccaggcc aacgtaacaa
    27421 cgcttcagcg ccgatacatt tttctgtttt gatatcgatt attggctggt aataaagaca
    27481 aagttgatgt ttttcgaggg cgcgttgcaa tttacgtttg ggagataaat agttttgtcg
    27541 aatacgtagc cagagtaata gcagaacaag actccccaaa ataccggcgg gcaacgcaaa
    27601 aataagatga ttataaaaat gggttataag acgttgatat gaagtcgcaa caatggctgc
    27661 aattgggcgt tttgtcgaat aaattgtcgc atataaatag ccatttcttt gtacggttaa
    27721 atccttcaaa tgaatcagcg gagaaaacgt tgctgccgag gcctctttgc ttaacgagaa
    27781 aaaggttttc atcaccgtat catacacacc ccattgcaat gtcgggtcat cagacatcac
    27841 ttcactccag aagagagggt tgataaccgc cacataattt ccccgctgca tataggtcat
    27901 tttatagcca gagaaaaaag gcgtatcgcg gtaataatag atagaaacgt taggttcacg
    27961 cttataatcg gccggtgcaa tcgtatagcc gtttacaggc gctatcagcg atgagcataa
    28021 aaaatggtta tcacgaattc attaatataa agatagccac gaataatatt caacattcgc
    28081 ttttgatggg ctggagtgca aacttgcccc tgatagcgtt cagctgcatc gctcactaaa
    28141 tctgcctggc gaatgaccag ctcggattta tccagtgcca gttgagcaaa ggttcggagc
    28201 tgattattca cttctgattt tgcccaaagg aaggcaatcc atagcgaaag aatgatggga
    28261 aagagaacta aaaagataat gcccggcaac gccagtaatt ggtgtcgtgc acgatgactc
    28321 attgctatca atttcctgcc tgtttatacc cggccattgc aaggtgaaac cctggctacc
    28381 gacgtcttta gataaatctt acggtataaa tagcactttt gcctgatttg aaattgatac
    28441 ctttttaatc gtcacctgga tgaaaataat tcattgatat tcaaaattaa ttttctacag
    28501 gaatacagag gcggcgggaa gatatgccct taatgccact gaaaaaccac ataatttgta
    28561 cagcaataaa agtcacggcc taatatggct aacaaacaat caattgtgat atagttcaca
    28621 aaattaatga aacaaacaga gtgtttcatt tttgtgttct gcaagccccg cgatagccag
    28681 agtgtaccac gcctcccgtg aacaacgccg cgctgtccag ggcatcggct cttttcagag
    28741 gaattgtttg atggctaccc tcaccactgg cgtggttctt cttcgctggc aacttcttag
    28801 tgccgtaatg atgtttctgg ccagcacact caacatccgt tttcgtcggt ctgattatgt
    28861 cgggcttgcg gtgatcagca gcggtctggg cgttgtttcc gcctgttggt tcgcaatggg
    28921 gttgcttggc atcacaatgg cggacatcac cgctatctgg cacaacatcg agtcggtgat
    28981 gatagaagag atgaatcaga caccgccaca atggccaatg attttgacct gatacgtaga
    29041 agc
//
LOCUS       DAJTUP010000043        28762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTUP010000043 DAJTUP010000000
VERSION     DAJTUP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.043"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO468_001959"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO468_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR6259087.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLKRATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO468_001960"
     CDS             complement(2138..2584)
                     /locus_tag="OO468_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR6259088.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO468_001961"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO468_001961"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR6259089.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO468_001962"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO468_001962"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR6259090.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO468_001963"
     CDS             complement(5347..6009)
                     /locus_tag="OO468_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR6259091.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO468_001964"
     CDS             6182..6595
                     /locus_tag="OO468_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR6259092.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO468_001965"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO468_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR6259093.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO468_001966"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO468_001966"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR6259094.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO468_001967"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO468_001967"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR6259095.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO468_001968"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO468_001968"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR6259096.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO468_001969"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO468_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR6259097.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO468_001970"
     tRNA            complement(9861..9948)
                     /locus_tag="OO468_001970"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO468_001971"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO468_001971"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR6259098.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO468_001972"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO468_001972"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR6259099.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO468_001973"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO468_001973"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR6259100.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO468_001974"
     CDS             14005..14592
                     /locus_tag="OO468_001974"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR6259101.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO468_001975"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO468_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR6259102.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO468_001976"
     CDS             14984..15841
                     /locus_tag="OO468_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR6259103.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO468_001977"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO468_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR6259104.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO468_001978"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO468_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR6259105.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO468_001979"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO468_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR6259106.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO468_001980"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO468_001980"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR6259107.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO468_001981"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO468_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO468_001982"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO468_001982"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR6259108.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO468_001983"
     CDS             complement(22884..24023)
                     /locus_tag="OO468_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR6259109.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO468_001984"
     CDS             complement(24069..26165)
                     /locus_tag="OO468_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR6259110.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO468_001985"
     CDS             complement(26165..26911)
                     /locus_tag="OO468_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR6259111.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO468_001986"
     CDS             complement(26908..27552)
                     /locus_tag="OO468_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR6259112.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO468_001987"
     CDS             complement(27659..27964)
                     /locus_tag="OO468_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259113.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO468_001988"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO468_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR6259114.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         6954 a         7288 c         7574 g         6946 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttt gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag ac
//
LOCUS       DAJTUP010000044        28707 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTUP010000044 DAJTUP010000000
VERSION     DAJTUP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28707
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.044"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..410)
                     /gene="wcaD"
                     /locus_tag="OO468_001989"
     CDS             complement(<1..410)
                     /gene="wcaD"
                     /locus_tag="OO468_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR6259115.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQI"
     gene            complement(385..1602)
                     /gene="wcaC"
                     /locus_tag="OO468_001990"
     CDS             complement(385..1602)
                     /gene="wcaC"
                     /locus_tag="OO468_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR6259116.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1599..2087)
                     /gene="wcaB"
                     /locus_tag="OO468_001991"
     CDS             complement(1599..2087)
                     /gene="wcaB"
                     /locus_tag="OO468_001991"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR6259117.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2090..2929)
                     /gene="wcaA"
                     /locus_tag="OO468_001992"
                     /pseudo
     CDS             complement(2090..2929)
                     /gene="wcaA"
                     /locus_tag="OO468_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3022..5184)
                     /gene="wzc"
                     /locus_tag="OO468_001993"
     CDS             complement(3022..5184)
                     /gene="wzc"
                     /locus_tag="OO468_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR6259118.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNCIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5187..5630)
                     /gene="wzb"
                     /locus_tag="OO468_001994"
     CDS             complement(5187..5630)
                     /gene="wzb"
                     /locus_tag="OO468_001994"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR6259119.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5636..6775)
                     /gene="wza"
                     /locus_tag="OO468_001995"
     CDS             complement(5636..6775)
                     /gene="wza"
                     /locus_tag="OO468_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR6259120.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7084..7233
                     /locus_tag="OO468_001996"
     CDS             7084..7233
                     /locus_tag="OO468_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259121.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7434..9017
                     /locus_tag="OO468_001997"
     CDS             7434..9017
                     /locus_tag="OO468_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR6259122.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9469..11322)
                     /gene="asmA"
                     /locus_tag="OO468_001998"
     CDS             complement(9469..11322)
                     /gene="asmA"
                     /locus_tag="OO468_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR6259123.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNSQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11344..11925)
                     /gene="dcd"
                     /locus_tag="OO468_001999"
     CDS             complement(11344..11925)
                     /gene="dcd"
                     /locus_tag="OO468_001999"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR6259124.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12017..12658)
                     /gene="udk"
                     /locus_tag="OO468_002000"
     CDS             complement(12017..12658)
                     /gene="udk"
                     /locus_tag="OO468_002000"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR6259125.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12976..13218
                     /locus_tag="OO468_002001"
                     /pseudo
     CDS             12976..13218
                     /locus_tag="OO468_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13217..14158
                     /gene="mdtA"
                     /locus_tag="OO468_002002"
                     /pseudo
     CDS             13217..14158
                     /gene="mdtA"
                     /locus_tag="OO468_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14158..17280
                     /gene="mdtB"
                     /locus_tag="OO468_002003"
                     /pseudo
     CDS             14158..17280
                     /gene="mdtB"
                     /locus_tag="OO468_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17281..20346
                     /gene="mdtC"
                     /locus_tag="OO468_002004"
     CDS             17281..20346
                     /gene="mdtC"
                     /locus_tag="OO468_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR6259126.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20347..21762
                     /locus_tag="OO468_002005"
     CDS             20347..21762
                     /locus_tag="OO468_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259127.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            21759..23162
                     /gene="baeS"
                     /locus_tag="OO468_002006"
     CDS             21759..23162
                     /gene="baeS"
                     /locus_tag="OO468_002006"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR6259128.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23159..23881
                     /gene="baeR"
                     /locus_tag="OO468_002007"
     CDS             23159..23881
                     /gene="baeR"
                     /locus_tag="OO468_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR6259129.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24072..24404
                     /locus_tag="OO468_002008"
     CDS             24072..24404
                     /locus_tag="OO468_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR6259130.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24551..25912
                     /gene="yegQ"
                     /locus_tag="OO468_002009"
     CDS             24551..25912
                     /gene="yegQ"
                     /locus_tag="OO468_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR6259131.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26254..26571)
                     /locus_tag="OO468_002010"
     CDS             complement(26254..26571)
                     /locus_tag="OO468_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259132.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            26977..27876
                     /gene="yegS"
                     /locus_tag="OO468_002011"
     CDS             26977..27876
                     /gene="yegS"
                     /locus_tag="OO468_002011"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR6259133.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(27958..>28707)
                     /locus_tag="OO468_002012"
     CDS             complement(27958..>28707)
                     /locus_tag="OO468_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6259134.1"
                     /translation="KIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLEQKGVVTRF
                     HGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVILDSGSTTM
                     LIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGSIAERSLQD
                     INADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNRRGFNQVLP
                     IEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
BASE COUNT         6559 a         7637 c         7499 g         7012 t
ORIGIN      
        1 ataatttgcg ccatctccac cgccgccagc gccaccacca gtccgaccac cagataaaag
       61 aaacgtaata tcttacgatg gttgtgtggc gaaattgttt taaaacgaat actccacacc
      121 atgccaataa tgatcacaat atagacaaac agcatagttg aagtgacgta tttaccggca
      181 tccaacgact gaccaaacag gtagttaaac gccgtgagtc ccgcgccaat ccctaaggca
      241 atcatcaatt ttttaatgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa
      301 gtgacgatgg tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg
      361 tagatcagtg gcagcagcag gtagctacag attctgatag aagttgacat actcctccag
      421 catctgttgt ccactgtagg cggcgcgact gcgttggctg aactcagcca gcgtggtacc
      481 aaatatcgcc tgcgcgattt ccggtttgct taactgtacc agttgcagca cctcttcttc
      541 actgacggtt ttaccaccgg atttttgcag cacttcccgc gccgcatcgc tatgggtggc
      601 aatcaccggc acgccaatcg atagcgcctc acacaaaatc agcgggtagt tatcgacacg
      661 agaactgaac accagcgcat ccatctgatt gagcgcgctc atcaacttgc gcttgtcagt
      721 ttcaaagccg tgattaacca cgttgccagc ggtgaacggc gagaacttac caaaggtatg
      781 cagttcgatt ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagttttgcc
      841 gtcgtaacgc aggtcatgcg ccaccactgc gattttcggc ttgccctgag tttcgcgcac
      901 cggaggcaga tctgccagaa tcgcttcggt tgccatatca atgccattat tgataatccg
      961 gcaacgccct ggcccgtaca gactattgaa agcgtcagcc acatgctggc tgggggaaat
     1021 aaactgacag cccagcgcca gcatctcgcg gaataactgg cgtttgcccg ccaccagctg
     1081 gtgcgcgcga tcaatcttca ccggcggata gttatttaag gtcgggcatt tctggcagcc
     1141 cgttttccag ccttcgcaac cgtcggtaaa ggcgcagcgt ccggttacac tccagtggtc
     1201 gtgcagcgtc cagaccaggg tgacatccgg tttatggttt ttcacttttt cgcaaaagcg
     1261 caccacgctg ttaagattca accagtagct gtgcagcaca tgaaaatgca ggaccaccgg
     1321 acccggtgtg cgagtaatgg tgcgatataa ctcattgaaa ttgccgaaca gatcgcgatt
     1381 aaacagacga aacaacgcaa tgttcgccat cgcggtcatc cgcggcgtat gtttgatgac
     1441 ctgcggatag ttctgatggc tgacgctctc tttgccgcct ttgccgtagc cgtagacaaa
     1501 atgtgacgcc agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc
     1561 cccgccttcc gccagtcgca cattaaattg cagaatattc atttaatcac cttcactcgc
     1621 gctttttctc ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca
     1681 cccacggtga cgttgttacc aagcgtgata tcaccaagaa taatgacgtt ggcaccgagt
     1741 tcgacgccgt tgccaatgtg tggacatgcc atgttatcgg caccccgatt gccgatagtg
     1801 acgccgtggc gaatggtgaa atcatccccc gccaccacgt ttttattgat cactacggcg
     1861 taaccgtgat ggatagtaaa gcggcggcca atggtcgcgg cggcctggat ttcataaccg
     1921 aaaaagcatt cggtgataat gcgatacagc accagcagcg gggccgccca cagattgttg
     1981 aggacgttct ttttgcgcca caccgaacaa aaatgagcga cgcgataggc aagtaccatg
     2041 cagcacgggc gtaaactcca gctgttggcg cgcagatctt ccagcatctt tagcgccccc
     2101 gaataccatc agccagacgt ttgccgttac gcaccgacag cagcgtcaac aaggtgcgcc
     2161 aggtcatacg tttattgcgg atctggtaga gggtaaacag ctgatatttt ttactggcgc
     2221 ggtcgaattt gtctttgtgc ttgcggtaaa agtggaagta tccggagaat tttttcggtg
     2281 acgaggtgat ctgcatttcg ccgtgattga tatgcaggat ctgcgtcgcc tcttccactt
     2341 tccacggttc gccgtactcc accaccatcc gcaggaatat gtcgtaatcc tgtgctgctt
     2401 tcagttcggt atcgaacaaa cactctttga aacgccatgc ccaggtaaag acctgattgc
     2461 caatgatatt acgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta
     2521 agctcgccgg ttgggaataa acttctccct agcaaacgta gtcgttagcg tacaaaaagg
     2581 cgtgtgtgac cagttgctgt ttatgggcga ggaagacgct caggcggttg ggcgtccatt
     2641 catcatcatc atcgatcccg gtgatgtatt ccccttgcgc cagcataatc gcctggttac
     2701 gcaccgcgca cgccccgctg ttaatgtcgt tgtggatgta agtgatacgc ggatcgttga
     2761 gggcagtgac gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca
     2821 tctcccagtt gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt
     2881 ggcggttcca ggttggcata tagattgaga tcagcggatt gtctttcata ggttgttctc
     2941 ccggcttatg agctggtttg cttgccggat gcggcgtgag cgccttatcc ggcctacggg
     3001 gcggtgcgaa tgcaggcgtc gttatttcgc atccgactta tattcgtatt cgtaatagcc
     3061 ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc ctttcaccgg
     3121 aatacagttt tgctcaaagc ggctcagact ggtttccact tctttcaatg tgttgaccgc
     3181 ataacgcgcc accattaacg tggttccgac atgacgacca acaattgccg catcggtcac
     3241 tgccagaatc ggcggcgtat caatcaacac caggtcatag tttttactcg cccagttcac
     3301 cagttcggca aagcgttcgc tcattaacag ttcagaagga tttggcggca cctgaccgcg
     3361 cgggatcagg tcaaatttgg caatagaggt cggtttagca gcggtagtaa tatcgccctg
     3421 accaatcaga atctccgaca gcccattgac attattggtg cccaacagct cgtgggtgta
     3481 gcctttgcgc atatcgcagt cgatcaacaa cacgcgttta ttggtctggc tgatcaccgc
     3541 cgccaggttg gcgcagacaa aggttttacc gattgacggg ctaaccccgg tcatcatcaa
     3601 cacattgttc tgcgcctgca tcatcgcgaa gtgcagactg gtacgcagac tacggatggc
     3661 ttcaatcgcc agatcggttg gattccccac tgccagtagc tggctctgtt tatagcgttt
     3721 aacccctttg atggttttga cgctatcgcg cgctttctgc cactccgaca gcgggatgct
     3781 ggcatagacg ctgattccgt gttcttccag cacttgcggg ctttcgatgc cgcgattaaa
     3841 caacgagcgc agcagcacac ccacgataga gagcatcagg ccaaggataa tcgcgccgag
     3901 gataatcagc cctttcttcg gttttagcac gccaggctgg gtgattgccg ggtcaataat
     3961 gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt tattcagcag
     4021 ttgcatatag acctgctgac cagactcgac atcgcgggtc agacggacaa tttcctgctg
     4081 gtttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt ccagcgcctg
     4141 acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct tggagatttc
     4201 cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg aatcgagcac
     4261 cgctttcgct tcaagcggca gatcaacaga atctttatcc tgacggaagg cattcagttt
     4321 gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga ggaaagcgag
     4381 gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt tacgagcgat
     4441 gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca ggctcagtac
     4501 gcctgcgtct ttgccgttct ccgttaccgt caggctgttt tgcagttggt tgatcatccc
     4561 caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa tggcttcaac
     4621 catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg cgctaaagcc
     4681 gccatcactg ctcagggtgt agtttttgtc atccagcaca ttaagcgtaa acacctgatc
     4741 cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct gacgccccat
     4801 caggcgatcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcaccg caatatcgag
     4861 gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact ggatctcggc
     4921 gtccgatgca ggcggtttgt tggctaatgc cgagccgata tcctgcacca gcgaattgcc
     4981 gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgtgg cgaagaaagt
     5041 gtaaaccacg gcacagaggg caaatacgac ggtgatgcca atcacccacc agcgcgcttc
     5101 aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta ccggagcggc
     5161 atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc ctgcgcccac
     5221 tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttcccg gcttttgtga
     5281 tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac tttgccgcgc
     5341 atctccggtg ccatctcgca taagcgttcg atatggcgct tatccatggt caaaatcagg
     5401 tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc cagagacagt
     5461 tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac cagcgcgccg
     5521 agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg ttccgccatc
     5581 ggggaacggc aaatattgcc gacacagaca actaagatgt tgttaaacat gacgattacc
     5641 agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata gttggcagca
     5701 actgattgat cagacggttc cagcgggaaa ccggggcggt ggtgacatac accacgtcat
     5761 aaggttgcag gcggaattct gtcgccatca ccagcgacgt cgcatcggac atatccagct
     5821 ggtagatatt ggcaatcttg ccgttacgcc cgccctcgcc tttcagcgga cgaatgacaa
     5881 agatgccgct ggcgttggag gtggtcatgt cgataccttc ggcattggcc agagcttcag
     5941 tcagggtcat gccgctaaag tccattttca gggtgctctg tttcttcact tcacccatca
     6001 caaatacttt cagatcatca ttacgcggca cgtagagaat atcgccggga taaagcagtc
     6061 ggttctgatt gaggtcgccg ttttgcatca gcgcctgtaa agaaatgcgt tcttcacgac
     6121 cattgtgtgt tagcaccacg ttgcgccagt cagcggtgtc ggtcaggcca cctgcggcgt
     6181 tgatggcgtc gagaatggtc agtggtacgt tggtgatcgc ctgttgaccg gatttattca
     6241 cctgacctga gatataggcc ttttgtgagc ggaaggcggc gatattaacg tccacctgcg
     6301 ggtcagcgat gtacgtcgct aagcgcccgg taatatcact gcggatttca gcgagcgttt
     6361 tcccgactac gtgaaccttg ccgatatacg ggtaaaacat agtgccgtca ggctgtaccc
     6421 agttgccggt gtcgctggag ctgcggtact gaccagctgg cgtggtgagt tccgggtgat
     6481 cccagacggt gacattaaga acgtcccccg gcccgacgcg atactggtaa ttcgcgatct
     6541 cgctttccag cgtcatattg gggcgcgcta cattcgggcg tgggcgtaat tggtcaatca
     6601 ggcgcggggt cagcggataa acattcacca ttttgtcgag atcgaaatca gcgtcctgct
     6661 gtttgatgac gtctttgccc atcgtcgaca tattgctgcc cggaagtact gtgcaaccgc
     6721 ttatcaaggt tactgacacc aataatggca tcaatttcat tttggatttc atcattgttt
     6781 atttatcact ttggcagagt aattatcctg tgcactatta atagcaatgt cgccatgcac
     6841 atttaccttg cagttaattg aataaaaatt taactggcat cagtcctaaa aaaattgatt
     6901 tcatccgcag gctattgaca gaataattca gactggtctt tcaggcatcc agacacgcta
     6961 ccgcccctgg ctttttagct accaatacac tgattgagct taatttttca aaccctctct
     7021 gcatacaatc gttgatgaga aagggttatt gcggaaatta acttccgaat ataaggtgac
     7081 attatggtaa ttgaatattg gctcgccaat aatgcaggag gaagtgttac agctaacgga
     7141 atagcaggca aggtaacaat tcggcaattg gctattttta agaattatat taagtatcat
     7201 tcaatatggt ttttaggagt ttctttaggt tgacaatatt taatattgcg tctccacatg
     7261 cgatatttct taaataatgt tttattatta ccgcttttaa ttcagggata atgtgaggtt
     7321 attaccctca aataatatga gataaatagt gctgcaacat tgcatttttg ccccgattta
     7381 tccatgatcg aattgtgaca tttgtcatac aacgaatagg ttttgtactt actatggaat
     7441 ggattgccga tccgtctatc tgggccgggt taatcacgct gattgtgatc gaactggtcc
     7501 tcggcattga taacctggtc tttattgcca tcctcgccga aaaactaccg ccgaagcagc
     7561 gcgaccgcgc acgggttacc gggctgctgc tggcgatgtt aatgcgcctg ttactgctag
     7621 cgtcaatctc ctggctggtc accctgactc aaccgctgtt cagcttccgc tcgtttacct
     7681 ttagcgcccg cgacttaatc atgctgtttg gtggtttctt cctgttgttc aaagccacga
     7741 tggagctgaa cgaacggctg gaagggaaag acagcaataa tcccacacaa cgcaaaggtg
     7801 cgaagttctg gggcgtggtg acgcaaattg tggtactgga cgccattttc tcactcgact
     7861 cagtgattac cgccgtcggg atggtcgacc atttactggt catgatggcc gccgtggtta
     7921 tcgctatcag cctgatgttg atggccagca agccgttaac gcaatttgtt aacagtcacc
     7981 cgaccatcgt tattctctgc ttaagcttcc tgttgatgat tggctttagc ctggtggcag
     8041 aaggtttcgg cttcgtcatt ccgaaaggct acctgtacgc tgccattggt ttctcggtga
     8101 tgatcgaggc actcaatcag ttggctatct ttaaccggcg acgttttctt tccgctaatc
     8161 agacgctacg ccagcggact accgaagcgg taatgcgcct gcttagcggg caaaaagaag
     8221 atgcggaact ggacgccaaa accgcgtcca tgctggtgga tcatggaaac cagcagatct
     8281 ttaatccgca ggaacggcgg atgattgagc gggtacttaa tcttaaccag cgtaccgtaa
     8341 gcagcattat gacgtcgcgc cacgatattg agcatatcga tctcaacgcg ccggaagaag
     8401 aaatccgcca gttgctggag cgaaatcagc atacgcggct ggtggttacc gacggtgatg
     8461 acgcagaaga tttgctcggt gttgttcacg ttatcgacct gctacaacag tcactgcgcg
     8521 gcgaaccgct caacctgcgg gtgttgattc gccagccgct ggtgttcccg gaaaccttgc
     8581 cgttgttacc agccctggag cagttccgta atgcccgcac gcactttgct tttgtggtgg
     8641 atgagtttgg ctcggtggaa gggattgtga cattaagtga cgtcactgaa accattgccg
     8701 gtaacttacc gaacgaagtg gaagagatcg acgcccgcca tgatattcag aagaatgcgg
     8761 acggttcctg gacggcgaat ggtcatatgc cgctggagga tctggtgcaa tatgtgccgc
     8821 tgccgctgga tgaaaaacgt gaatatcaca ccattgccgg gctgttgatg gagtatttgc
     8881 agcgtattcc aaaacctggc gaagaagttc aggtggggga ttatttgctt aaaacgttgc
     8941 aggtggaaag ccatcgcgtg cagaaggtgc agattatacc gctgcgtaag gatggcgaga
     9001 tggagtacga ggtgtgatgg tatcggccat tttacccaat gctgcgtaac gccttataac
     9061 ttcagcaatg aagcgttgtc tgtggtaaac ggacatcaca gcgggggtgt gggtccggct
     9121 gtaaatcgcc gaatcgtttc ctgtaggctg gggcagccca catggatgtg ggctgagggc
     9181 gcgttttaca gggatgttac ctcgcgcccg tacccgatag ccgaaaggaa taagatgagg
     9241 gaaccgcgcc agcggcgatt taccgccggg agcccgggtt gccagggtgg tggcgattga
     9301 gccaccctgg cacgttcacc ggcttgagcg ttccatagta gcgaggaaca tcaagtgaac
     9361 ggaacagcct tcgatcttac atattcagaa tataatagtg ccgccgcgaa cgcctgatgc
     9421 gacgctttac gcgtcttatc aggcctacag gacaggtcac ctggtaacct acatcttctc
     9481 cagcaacttc ttcacatctt tgccattgcg ggaatcttta ttccgctcgg cccagtcatt
     9541 caggcgacgt ttcgcttcgt cctgtagatg tttgcgcagt aactgatcca cttgcaggct
     9601 gtaattgagt tgctgccagt tgccataaac ccgcagcggt actggcgttt ctttgaggaa
     9661 atcaatcagt ttgctttccc cgttccagcc acccacgacc cgaatatcaa actgggtgtc
     9721 gcaggtttga tctgccagat tcaacatgcc ttcccctgtc agcgccagca caggcgattg
     9781 accttgcatg tcgtttaacg tcacgacgcc atccttcaac gtcaaatcgg tggtaaagcg
     9841 gtcaagccgc gtcacgttat cgaagttttc agcggccttc acatcaccgc cattacgctc
     9901 taccgcttgc tgaatcatct gctgggagtt catcccttcc atacgtgtgt cggtcatctc
     9961 aacatgcgcc tgtccttgcc agttgtggcg gaatgcgtcg gcatctatgt cagcaccgga
    10021 gaaatcacca gccagtgaca tttttccggt caacgaaatc ggatagttaa acgccttcag
    10081 aatggtacca atctcaacgt tctccagccg tggctggaag tttatccgcg gatttattga
    10141 tgtcgcgtcc agcgtgcccg gcagtgaaac ctttccaccg ttaagcttgc cctgcagttg
    10201 agtaatttcc agcaaacccg acttgttggt catttgcgtg gcaacatctg taaaattcat
    10261 tccgcgccag cgcacgttac tggcctgcaa caaaatatca gctgtaaagc cttgcagtcc
    10321 ctgataggcc ggttcatcaa tacgcgaaga aatgaccggg cgcggcaacg ttgattggct
    10381 ctgcccctgc tgcgcggcac cgttttcacc attcgcggtt tcattaagcg ggatgaggtt
    10441 gtcaagattc agttgcggga attgcagtct cagctgccat tccggtttct ctgtcagcgt
    10501 gacctgtgct tgtccgctca gcgtactgtc attggcggtc aaactaattt ggttaaatga
    10561 aaggcgttta tgtgactcct gccactggga ctggaaactc ccctgcccct gaataccttg
    10621 ttttggtaaa tcggcaccct gcaactgcca gttaatttgt tcaatagccg ctgttaaatc
    10681 atgcggataa tcagaagcat ctaccgtacc gttaagggat atcgtgagat cgcgctgatc
    10741 gcgattaacc cgcccggaga actcaaatga gccacgatgt tggggatctt gttccatttg
    10801 caggcggata ttgcggattg tcacctgctc gtcatcttca tgctggaaca ccagaacgct
    10861 gtccgccacc ttaagactgg atatatcaaa cgaccatccg cgatcgtctg acagatccgg
    10921 caaggtattg tcgcgcggtg caaccggagc gtcttcactg cgcaccgctt ccgtctgcgg
    10981 cgtcagttgg atcactgccc cttttagcat cacctgctta acgctcagtt gatgactcag
    11041 tagtggtaaa agcgccacgt ccagacgcat gttgtcggcg cgaaccagtg gctggcttgc
    11101 gccctgggcg gtgagagaca ttcgcccgga gaggatacta agctgcggcc agacgtgcca
    11161 acgcagtggc ccgtcgagct gcaattgata accgctacgt gcagcaactt gcttgaccat
    11221 atagtcgcgg aaatcattcg gattcaccag caacactaac gcagataacc cggccaccag
    11281 cacgaccaag agtatcatca gcgtcgtcag aaatcgtctc atggtatcct caatgggcct
    11341 gaattagtct ttatcgattc ggctggctac cgcgccctgc tggttgcgat atttcgcatc
    11401 ttcacggcgg ttgtaaggtc gcgccgccgg gccggaaagc ggctcaaagc tcagcgcacc
    11461 aattaacatg cccggacgca gcgccagcgg cagcttaccg gagttgtaga actccagcac
    11521 aatgcaacca gaccagcccg gatcgatacg gtgcgcggtg acgtgcacca tcagccccag
    11581 acgcgccaaa gaggaacgcc cgtccagcca gcccaccaga tcggctggca gcgtcaccga
    11641 ctccagtgtc accgccagcg ccagctctcc tgggtgaaga tagaacgcct cgccctcgtc
    11701 gagaacgatc tcatcgctca tcacgcggtc aagcgcggcg ctcacttcat ctttgggacc
    11761 gctcagatcg ataaacgctg ccgtgtgacc acggaaggta cgaaatttat tgcccaggcg
    11821 tacatccacc gtcgcgccgt taatacgctc cactggcgga cgtgggttga tcgacaaacg
    11881 gccttcatca agccaggctt caatatctcg gtcacacaga cgcatggcat tttctccttt
    11941 cgcacatcac tcccttaacg ccaattacgt caagggcata ccaacttatc actgaacggt
    12001 acacaattta tcaagcttat tcaaagaact gacttatttt cgctttcaat atatcgatcg
    12061 cgatgcggtt tttcccgccg cgcggcacga taatgtccgc atattgttta gaaggctcaa
    12121 tgaattgcag gaacatcggg cgcacggttt tttgatattg cgccatcacg gaatccattg
    12181 aacgcccacg ctcgttaacg tcacgcttga tgcggcgcat caggcagata tccagcgggg
    12241 tatcaacgaa aatggagaag ttaagttcgt cacgcaaacg cgcatccgtc agcaacaaaa
    12301 tgccttcgag aatgatgacc ttcttcggct caaccgtcac cgtttctttc atacgcgtat
    12361 gttcaacata gctgtaaacc ggcaggtcaa ttgccgagcc gcgtttcaac gcttgtaaat
    12421 gctcaagcaa caggctgtga tccatcgcgc tgggatggtc gtagttggtc ttaacgcgtt
    12481 cttccatcga cagatggctt tgatctttgt aatagcagtc ttcgggaatt acgccgatgt
    12541 gttcatcacc gacttgctca cgtaattcac gataaagggt actggcaata agactcttgc
    12601 cggaagccga tgcgccagcg ataccgataa tgacgcactg atgagactga tcagtcatat
    12661 atttagcgac ctgattaacc tggatgttag gaagggggcg acgaagcgcc aaacgcggca
    12721 attataggga tttcatccgt ctgataccag tcgaatagcg ttgccgcgcg ctcagagtta
    12781 attgttgaca aagaattccc ggtggcaaat tacgttgatc agttttatgt aaggtaaaaa
    12841 atgttatacg cagttgcgca aattatccgc ctttacgtca ctttatgagc aattcgcata
    12901 taaaatgtaa aacttttgta ctagcataaa cacagaaacg aatactggcg acctggcctt
    12961 gcggataaag cggtaataag caaacaatca cagcatgtat taattgccct gccccacccg
    13021 ctgcttcacc tggtcagttt aggtttagtc tcgtttatct ttacccttgt ctcgcttgag
    13081 ctttcgcagt ttggcaccca actcgcccca ctgtggttcc cgacgtccat catgatggtg
    13141 gcgttttatc gccatgccgg gcgcatgtgg ccgggaattg cgctgagctg ttcgctggga
    13201 aatatcgccg catccattcc aggaaggcca gcaggtcaaa gcaggcgatt tactggcaga
    13261 aattgacccc agccagttca aagttgcatt agcacaaacc cagggccaac tggcaaaaga
    13321 taaagccacg cttgctaacg cccgccgtga tctggcgcgt tatcagcaac tggcaaaaac
    13381 caatctcgtc tcccgtcagg agctggatgc ccaacaggcg ctggtcagtg aaaccgaagg
    13441 caccattaag gctgatgaag caagcgtcgc cagcgcgcag ttgcaactcg actggagccg
    13501 catcaccgca ccagtcgatg gtcgcgttgg tctcaagcaa gttgatgttg gtaaccaaat
    13561 ctccagtggt gataccaccg gaattgtggt gatcacccag acgcatccta tcgatttagt
    13621 ctttaccctg ccggaaagcg atatcgctac tgtagtgcag gcgcaaaaag ccggaaaacc
    13681 gctgatggta gaagcctggg atcgcaccaa ttcgaagaaa ttaagtgaag gcacgctgtt
    13741 aagcctcgat aaccaaatcg atgccacaac cggtacgatt aaagtgaaag cacgctttaa
    13801 taatcaggat gatgcgctgt ttcccaatca gtttgttaac gcgcgcatgt tagtcgacac
    13861 cgaacaaaac gccgtagtga tcccaacagc cgccctgcaa atgggcaatg aaggccattt
    13921 tgtctgggtg ctgaatagcg aaaacaaggt cagcaaacat ctggtgacgc cgggcattca
    13981 ggacagtcag aaagtggtga tccgtgcagg tatttctgcg ggcgatcgcg tggtgacaga
    14041 cggcattgat cgcctgaccg aaggggcgaa agtggaagtg gtggaagccc agagcgccac
    14101 cactccggaa gagaaagcca ccagccgcga atacgcgaaa aaaggagcac gctcctgatg
    14161 caggtgttac ccccgagcag cacaggcggc ccgtcgcgcc tgtttattat gcgtcctgtc
    14221 gccaccacgc tgctgatggt ggcgatctta ctcgccggga ttatcggtta tcgcgccctg
    14281 cccgtttcgg cgctgccgga agtggactat ccgaccattc aggtggtcac gctctaccca
    14341 ggtgccagcc cggatgtcat gacctctgcc gttaccgcgc cgctcgaacg ccagttcggg
    14401 cagatgtctg gcctgaaaca gatgtcgtcg caaagttccg gcggtgcgtc agttatcact
    14461 ttgcagttcc agctaacatt accgctcaat gtcgccgagc aggaagtgca ggccgcgatt
    14521 aacgctgcga ccaacttgtt gccgagcgat ctgcctaacc cgccggttta cagcaaagtg
    14581 aacccggcag atccgccgat catgacgctc gccgtcacct caaccgccat gccgatgacg
    14641 caagtggaag atatggtgga aacccgcgtc gcgcagaaaa tctcgcagat ttccggcgtc
    14701 ggcctggtga cgctttccgg cggtcagcgt ccggctgttc gcgtcaaact taacgctcag
    14761 gcgattgccg ccctcggcct gaccagcgaa accgtgcgca ccgccattac cggcgctaac
    14821 gttaactcgg caaaaggtag cctcgacggc ccttcccgtg cggtcacgct ttccgcgaac
    14881 gaccagatgc aatccgccga agagtatcgc cagctaatca tcgcctacca gaacggcgcg
    14941 ccaattcgtc tgggcgatgt cgcaactgta gagcaaggtg cagaaaacag ctggctcggc
    15001 gcgtgggcga acaaagaaca ggccattgtg atgaatgttc agcgccagcc cggtgctaac
    15061 attatctcca ccgccgacag cattcggcag atgctgccac agctcactga gagtctgccg
    15121 aaatcggtga aggtgacagt gctttccgat cgcaccacca atatccgcgc atccgtcgat
    15181 gatactcagt ttgaattgat gatggctatc gcgctggtag tcatgattat ctacctgttt
    15241 ttgcgcaata ttccggcgac catcattccc ggtgttgctg taccgctgtc gttaatcggc
    15301 actttcgcgg ttatggtgtt tctcgatttt tcaatcaata acctgacact gatggcttta
    15361 actatcgcca ccggattcgt ggtcgatgac gccatcgtgg tgatcgaaaa catttcccgc
    15421 tatatcgaaa aaggcgaaaa accgttggcg gcggcgctca agggcgcagg tgaaatcggc
    15481 tttaccatta tctcgctgac cttctcactg attgcggtgt tgatcccact gctgtttatg
    15541 ggcgatatcg tcgggcgact gttccgcgaa tttgctatta ccctggcggt agcgattttg
    15601 atctcagcgg tggtgtcgct gaccctgaca ccgatgatgt gcgcgcggat gctcagccag
    15661 gagtcgttgc gtaaacagaa cagcttctcc cgtgcctcgg aaaaaatgtt cgacaggata
    15721 atcgccgcct atggtcgtgg actggcgaaa gtgctgaatc atccgtggct gaccttaagc
    15781 gtggcactca gcacgctgct gcttagcgtg ctgctgtggg tgttcattcc gaaaggtttc
    15841 ttcccggtac aggacaacgg cattattcag ggcactttgc aggcaccgca atccagctcc
    15901 tttgccaata tggcccagcg acaacgccag gtcgcggacg tgattttgca ggatccggca
    15961 gtgcaaagcc tgacctcatt tgttggcgtt gatggcacta acccgtcgct gaatagtgca
    16021 cgtttacaaa tcaacctcaa accgttggat gaacgtgatg atcgggtgca aaaagtcatc
    16081 gcccgtctgc aaacggcggt agataaagtg ccgggcgtcg atctcttcct gcaaccaacg
    16141 caggacctga ccatcgatac tcaggtcagc cgcacccagt accagtttac cttgcaggcc
    16201 acgtcactgg atgcgctcag tacctgggtg ccacagttga tggaaaaact ccagcaactg
    16261 ccacagcttt ctgatgtctc cagcgactgg caggacaaag ggctggtggc gtatgtcaat
    16321 gttgatcgcg acagcgccag ccgtctgggg atcagcatgg cggacgtcga taacgccctg
    16381 tacaacgcgt ttggtcagcg actgatttcc actatttata ctcaggctaa ccagtatcgc
    16441 gtggtgctgg agcacaatac cgaaaatacc ccaggcctcg cggcgctgga taccattcgc
    16501 ctgaccagca gcgacggtgg cgtggtgccg ttaagctcaa ttgccaaaat tgagcagcgt
    16561 tttgcgccgc tttccatcaa ccatctggat cagttcccgg taacaaccat ctcctttaac
    16621 gtgccggata actattcgct gggcgatgcg gtgcaggcga ttatggacac cgaaaagacg
    16681 ctgaatctgc cggtggatat caccacgcaa ttctagggca gcaccctcgc cttccagtcg
    16741 gcgctgggca gcactgtctg gctgattgtc gcggcggtgg tggcgatgta tatcgtgctc
    16801 ggcattctgt acgagagctt tattcacccg atcaccattc tctcgacgct acccaccgca
    16861 ggggtcggcg cgctgctggc attaatgatt gccggtagcg aactggatgt gattgcgatt
    16921 atcggcatta ttttgctgat cggtattgtg aagaagaacg ccatcatgat gatcgacttc
    16981 gcgctggctg ctgagcgcga gcaaggcatg tcgccgcgcg atgcaatcta ccaggcttgt
    17041 ctgttgcgtt ttcgtccgat cctgatgacc accctggcgg ctctgcttgg cgcgctgccg
    17101 ctgatgttga gtaccggggt cggcgcggaa ctgcgtcgtc cgttaggtat cggcatggtc
    17161 ggcggtctga ttgtcagcca ggtgctgacg ctgttcacca cgccggtgat ttatctgctg
    17221 ttcgaccgcc tggcattgtg gaccaaaagt cgctttgctc gccatgaaga ggaggcgtaa
    17281 gtgaagtttt ttgccctctt catttaccgc ccggtggcga cgattttact gtcggtcgcc
    17341 attaccctgt gcggcatact gggtttccgt atgctgccgg tcgccccgct gccgcaggtc
    17401 gattttccgg tgattatggt cagcgcctcg ctgcccggtg cgtcgccgga gacaatggcg
    17461 tcttccgttg ccacgccgct ggagcgctca cttgggcgca ttgccggggt tagtgaaatg
    17521 acctccagca gttcgctcgg cagcacgcgc attattttgc agtttgattt tgaccgggat
    17581 atcaacggcg cagcgcgtga tgtgcaggcg gcgatcaacg ctgcacaaag tttgctgccc
    17641 agcgggatgc ccagccgccc gacctatcgc aaagcgaacc cgtcggatgc gccaattatg
    17701 atcctcacgc tgacatccga tacttattcg cagggtgaac tgtacgattt cgcctcgacg
    17761 cagctggctc cgacgatttc gcaaatcgac ggtgtcggtg atgtcgatgt cggtggcagc
    17821 tcgctgcccg ccgtgcgcgt cgggctgacc ccgcaggcgc tgtttaatca gggcgtgtcg
    17881 ctggacgacg tacgcactgc catcagcaat gccaacgtgc gtaaaccgca gggggcgctg
    17941 gaagatggca ctcaccgctg gcagatccag accaacgatg aactgaaaac cgccgctgaa
    18001 tatcagccgc tgattattca ctacaacaac ggcggcgcgg ttcgtctggg cgatgtggcg
    18061 acggtgactg actcagtgca ggatgtgcgc aacgccggga tgaccaacgc caaaccggct
    18121 attttactga tgatccgcaa actgccggaa gccaatatta tccagacggt agacagcatc
    18181 cgggcaaaat taccagagtt gcaggagacc attccggcgg cgattgatct gcaaattgcc
    18241 caggatcgct cccccaccat tcgcgcctcg ctggaagaag tcgagcaaac gctgattatc
    18301 tcggtggcgc tggtgattct ggtggtcttt ttattcctgc gctcgggtcg cgccactatt
    18361 attcccgccg ttgcggtgcc ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc
    18421 ggattcagtc tcaataatct ttcgctgatg gcgctcacca ttgctactgg tttcgtggtg
    18481 gatgacgcca ttgtggtgct ggaaaacatt gcccgtcatc tggaagcggg gatgaaacca
    18541 ctacaggccg cgctgcaagg tactcgcgaa gtcggcttta cggtgctgtc gatgagtctg
    18601 tcactggtgg cggtgttcct gccgctgctg ttgatgggcg gattgccggg ccgactgtta
    18661 cgcgagtttg ccgtgacgct ttctgtcgcc attggtattt cgttgctggt ttccctgacg
    18721 ctaacgccaa tgatgtgcgg ctggatgcta aaagccagca agccgcgtga gcaaaagcga
    18781 ctgcgtggtt ttggccgcat gttggtagcc ctgcaacaag gctacggcaa gtcgctgaaa
    18841 tgggtgctca atcatacccg tctggtgggc gtggtgctgc ttggcaccat tgcgctgaat
    18901 atctcgatcc cgaaaacctt cttcccggag caggacaccg gcgtactgat gggtgggata
    18961 caggcggatc agagtatttc gtttcaggcg atgcgcggca aattgcagga tttcatgaaa
    19021 attatccgtg acgatccggc agtggataat gtcaccggct ttaccggcgg ttcacgagtt
    19081 aacagtggga tgatgtttat caccctcaag ccacgcgacg aacgcagcga aacggcgcag
    19141 caaattatcg accgtctgcg cgtgaagctg gcgaaagaac cgggggcgaa tctgttcctg
    19201 atggcggtac aagatattcg cgttggtggg cgtcagtcga acgccagcta ccagtacacg
    19261 ttgttatccg acgacctggc ggcactgcgc gaatgggagc cgaaaatccg caaaaaactg
    19321 gcgacgttgc cggaactggc ggacgtgaac tccgatcagc aggataacgg cgcggagatg
    19381 aatctggttt acgaccgcga caccatggca cggctgggaa tcgacgtaca ggccgccaac
    19441 agtctgttaa ataacgcctt cggtcagcgg caaatctcga ccatttacca gccgatgaac
    19501 cagtataaag tggtgatgga agtggatccg cgctataccc aggacatcag tgcgctggaa
    19561 aaaatgttcg ttatcaataa cgaaggcaaa gcgatcccgc tgtcgtattt cgctaaatgg
    19621 caaccggcga atgccccact atcggtgaat catcagggat tatcggcggc ctcgaccatc
    19681 tcgttcaacc tgccgaccgg aaaatcgctc tcggacgcca gtgcggcgat cgatcgcgca
    19741 atgacccagc ttggtgtgcc ttcgacggtg cgcggcagtt ttgccggcac ggcgcaggtt
    19801 ttccaggaga cgatgaactc gcaggtgatc ctgattatcg ccgccatcgc cacggtgtat
    19861 atcgtgctgg gtatccttta cgagagttac gtacatccgc tgacgattct ctccaccctg
    19921 ccctcggcgg gcgttggagc gctgttggcg ctggagctgt tcaatgcccc gttcagccta
    19981 atcgccctga tagggatcat gctattaatc ggcatcgtga agaaaaacgc cattatgatg
    20041 gtcgattttg cgcttgaagc ccaacggcac ggtaacctga cgccgcagga agctattttc
    20101 caggcctgtc tgctgcgttt tcgcccgatt atgatgacca ccctggcggc gctgtttggc
    20161 gcgctgccgc tggtattgtc gggcggtgac ggctcggagc tgcggcaacc cctggagatc
    20221 accattgtcg gcggtctggt gatgagccag ctcctgacgc tgtataccac gccggtggtg
    20281 tatctctttt tcgaccgtct gcggctgcgt ttttcgcgta aacctaaaca aacggtaacc
    20341 gagtaaatga cagatcttcc cgacagcacc cgctggcaat tatggattgt ggctttcggc
    20401 ttctttatgc agtcgctgga caccaccatc gtaaacaccg cccttccctc aatggcgcaa
    20461 agcctcgggg aaagtccgtt gcatatgcac atggtcattg tctcttatgt gctgaccgtg
    20521 gcggtgatgc tgcccgccag cggctggctg gcggacaaag tcggcgtgcg caatattttc
    20581 tttaccgcca tcgtgctgtt tactctcggt tcactgtttt gcgcgctttc cggcacgctg
    20641 aacgaactgt tgctggcacg cgcgttacag ggcgttggcg gtgcgatgat ggtgccggtc
    20701 ggcaggttga cggtgatgaa aatcgtaccg cgcgagcaat atatggcggc gatgaccttt
    20761 gtcacgttac ccggtcaggt cggtccgctg ctcggtccgg cgctcggcgg tctgctggta
    20821 gaatacgcgt cgtggcactg gatctttttg atcaacattc cggtggggat tatcggtgcg
    20881 atcgccacat tgatgttaat gccgaactac accatgcaga cgcggcgctt tgatctttcc
    20941 ggctttttat tgctggcggt tggcatggcg gtattaaccc tggcgctgga cggcagtaaa
    21001 ggtacaggtt tatcgccgct ggcgattgct ggcctggtcg cagttggcgt ggtggcactg
    21061 gtgctttatc tgctgcacgc cagaaataac aaccgcgccc tgttcagtct gaaactgttc
    21121 cgcactcgta ccttttcgct gggcctggcg gggagctttg ccggacgtat tggcagtggc
    21181 atgttgccct ttatgacacc ggttttcctg caaattggcc tcggtttctc gccgtttcat
    21241 gccggactga tgatgatccc gatggtgctt ggcagcatgg gaatgaagcg aattgtggta
    21301 caggtggtga atcgctttgg ttatcgtcgg gtactggtag cgaccacgct gggtctgtcg
    21361 ctggtcaccc tgttgtttat gactaccgct ctgttgggct ggtactacgt tttgccgttc
    21421 gtcctgtttt tacaagggat ggtcaactcg acccgtttct cctccatgaa caccctgacg
    21481 ctgaaagatc tcccggacaa tctggcgagc agcggaaaca gcctgctgtc gatgattatg
    21541 caattgtcga tgagtatcgg cgtcaccatc gccgggctgt tgctggggct ttttggttcg
    21601 cagcatatca gcgtcgacag cggcaccaca caaaccgtct ttatgtacac ctggcttagc
    21661 atggcgttta tcatcgccct tccggcgttc atctttgcca gagtgccgaa cgatacgcat
    21721 caaaatgtag ctatttcgcg gcgaaaaagg agcgcgcaat gaagttctgg cgacccggta
    21781 ttaccggcaa actgtttctg gcgattttcg ccacctgcat tgtcttgctg atcagtatgc
    21841 actgggcggt gcgtatcagt tttgagcgtg gctttattga ttacatcaaa cacggcaatg
    21901 aacagcgatt acagctgtta agtgatgcgc ttggcgagca gtatgcgcag catggtaact
    21961 ggcgcttcct gcgcaacaac gatcgctttg tctttcagat cctgcgttca tttgaacacg
    22021 ataattcgga agataaaccc ggcccgggta tgccgccgca cggctggcgt acccagttct
    22081 gggtggtcga tcaaaacaac aaagtactgg ttggtccgcg agcgccgatt ccacctgacg
    22141 gtacacggcg acccattctg gtcaacggtg cggaagtggg cgcggtgatt gcctcccctg
    22201 ttgagcggct gacccgcaat actgatatca atttcgataa acaacagcgg caaaccagct
    22261 ggctgatcgt cgccctggca acgttactcg cggcgctcgc cacctttctg ctggcgcgcg
    22321 gtttgctggc accggtaaaa cgacttgtcg atggcacgca caaactggcg gcgggcgatt
    22381 tcactacccg cgtaacgccc accagtgaag atgaactggg caaactggcg caagacttca
    22441 accagctcgc cagcacgctg gagaaaaacc agcaaatgcg gcgcgatttt atggccgata
    22501 tttctcacga actgcgtacg ccattagcgg tgctgcgcgg tgaactggaa gccattcagg
    22561 atggcgtgcg caaattcacg ccggagacgg tggcgtcttt acaggcggag gtcggtacac
    22621 tgaccaaact ggttgacgat cttcatcagt tgtcgatgtc tgatgaaggc gctctcgcct
    22681 atcaaaaagc accggtagat ttgatcccac tgctggaagt ggcgggcggc gcatttcgcg
    22741 aacgattcgc cagccgtggc ctgaaactgc aattttccct gccagacagt atcaccgtat
    22801 ttggcgatcg cgaccgttta atgcagttat tcaataactt actggaaaac agcctgcgct
    22861 acactgacag cggcggcagc ctgaaaatct ctgccgagca gcacgacaaa acggtgcgcc
    22921 tgacctttgc cgacagtgcg ccaggtgtca gtgacgatca gctacaaaaa ttgtttgaac
    22981 gtttttatcg caccgaaggt tcccgcaacc gcgccagcgg cggttccggg ctggggctgg
    23041 cgatttgcct gaacattgtt gaagcccata atggccgcat tattgccgcc cattcgcctt
    23101 ttggcggggt aagcattaca gtagagttac cgctggaacg ggatttacag agagaagtat
    23161 gaccgagtta ccaatcgacg aaaacacacc gcgtattttg atcgtggaag atgaaccgaa
    23221 gctggggcag ttgctcattg attatctgcg tgctgcgagc tatgcgccga cgcttatcag
    23281 ccacggcgat caggtactgg cgtatgtgcg ccagacacca ccggatctga tcctgttgga
    23341 tctgatgctc cctggcaccg atggcctgac attatgccgg gaaattcgtc gtttttccga
    23401 tgttccgatc gtgatggtga cggcaaaaat cgaagagatc gaccgcctgc tggggctgga
    23461 gattggcgcc gatgactaca tctgcaagcc gtacagccca cgggaagtgg tagcgcgtgt
    23521 caaaaccatt ttgcgccgtt gtaaaccgca acgcgaactt cagcaacagg atgccgaaag
    23581 cccgttgatt atcgacgaag gtcgttttca ggcttcatgg cgcggtaaaa tgcttgacct
    23641 gacgcctgcg gaatttcgtc tgcttaaaac gctctctcac gaaccaggaa aagtgttctc
    23701 ccgcgagcaa ttgctcaatc atctttatga cgactaccgc gtagtaaccg accgcaccat
    23761 tgacagccac attaaaaatc tgcgccgcaa gctggaatct ctcgacgccg aacagtcatt
    23821 tatccgcgcc gtttacggcg ttggttaccg ctgggaagcc gacgcctgcc gcatcgttta
    23881 gttttagcga cattactttg ttagccggag atggcgttca gacccgatcc ggcaacatta
    23941 ttccacgcat ggtcagcaaa ctgacattat ctctccgccc tggcaggcat cagaaaatcg
    24001 ctcatacttt aatcagtaaa cagcaccttt agatgctgtt ttcgataagc aatttcaatc
    24061 aaggagtcat tatggctggt tggtttgaac tcagtaagag cagtgataat cagttccggt
    24121 ttgtgctaaa agcgggcaat ggtgagacta tcctcaccag cgagctgtat acctcaaaag
    24181 cctctgcgga aaagggcatc gcgtcggtgc gtagcaacag cccgcaagaa gagcgctatg
    24241 agaaaaaaac ggcaagcaac ggcaaattct atttcaatct gaaagccgct aatcatcaaa
    24301 ttatcggctc cagccagatg tacgccaccg cgcaatctcg tgaaaccgga attgcctccg
    24361 ttaaagccaa tggcacaagc cagacggtga aagacaatac gtaattacga tgccgggcgt
    24421 gggttgttgg cgtccggcgc tttaccagat cccccctaca gcgctacaat gcccgccctt
    24481 aaagtggggg cctcccctaa ccgcttcatc aggtgaagcg gatctgacct gtcatcagaa
    24541 cgagagaatt atgtttaaac cggaactcct ttccccggcg ggaacgctga aaaatatgcg
    24601 ttacgctttc gcttatggcg cagatgctgt ttatgcgggc cagccgcgtt actccctacg
    24661 tgtgcgcaac aacgaattca accacgaaaa tcttcagctc ggcatcaatg aagcccacgc
    24721 gctggggaaa aagttttatg tcgtggtcaa cattgcaccg cacaacgcca agctgaaaac
    24781 ctttatccgt gacctgaaac cggtggtgga aatggggccg gatgcgctga ttatgtccga
    24841 tccagggctg attatgctgg tgcgtgagca cttccctgaa atgccgatcc acctctcggt
    24901 gcaggctaac gccgtgaact gggcgacggt gaaattctgg cagcaaatgg gactgacccg
    24961 cgtgatcctc tctcgcgagc tgtcgctgga agagattgaa gagatccata atcaggtgcc
    25021 ggatatggag atcgagatct tcgttcacgg cgcactgtgc atggcctact ccggtcgctg
    25081 cctgctctct ggctatatca acaagcgcga cccgaaccag ggcacctgca ccaacgcctg
    25141 ccgctgggag tacaacgtcc aggaagggaa agaagatgac gttggcaaca tcgtacacaa
    25201 gtacgagccg attccggtgc aaaatgttga gccgacgctg ggtatcggcg cgccaaccga
    25261 caaagtgttt atgatcgaag aagcccagcg tccgggcgag tatatgaccg cgtttgaaga
    25321 tgagcacggc acttacatca tgaactcgaa agatctgcgc gccatcgccc atgtagaacg
    25381 cctgaccaaa atgggcgtgc attcgctgaa aatcgaaggt cgtaccaaat ctttctacta
    25441 ttgcgcacgc accgcacagg tttaccgcaa agctatcgat gacgccgctg cgggaaaacc
    25501 gttcgatacc agcctgctgg aaactctgga aggtctggcg catcgtggct ataccgaagg
    25561 tttcctgcgt cgtcatactc acgacgatta tcagaactac gaatacggtt attcagtttc
    25621 tgaccgccag cagtttgttg gtgagtttac cggtgagcgc aagggggacc tcgcagcggt
    25681 agcggtgaaa aataaattct ccgttggcga cagtcttgag ctgatgacgc cgcaaggcaa
    25741 cattaacttt acccttgagc acatggaaaa cgccaaaggc gaagctatgc cggtcgcacc
    25801 aggcgatggt tatactgtgt ggctcccggt cccgcaggat cttgagctaa attacgcgct
    25861 gctaatgcgt aatttctccg gggaaaccac gcgtaacccc cacggtaagt gattaatttc
    25921 gattattttt cccggatgga aaattcttag aaaccgatca catacagctg catttattaa
    25981 ggttatcatc cgtttcgctg aaaaacataa tccataaaat gctagctgta ccaggaacca
    26041 cctccttagc ctgtgtaatc tcccttacac gggcttattt tttgtacata agctgtaaag
    26101 aaggattgat ccctgataac cgtcttccat tgaccacatc gatagaatcc cccttcatag
    26161 cacaatgcct ttcacgtaac ggtatcgtgc tcgcccaggt tccggctacg cacagccaga
    26221 acgcgcatgt ttgacgctta caaaaaaata ttctcactct ccacatttga atgtcagacg
    26281 agcgacgcca tgtaatcctg caccttctgt cttcaggtca actatctgca tttttttgcc
    26341 ctgagtaaca cagaaatggg ctgcatcatt ttttactata ttttctgcac cagatattct
    26401 gcccctggct aaagaagctt cggcttcggt gtagtattgg ttatcgagtt tacgctgaat
    26461 attactttta tatgcaagac caaatttacc gatacttgtt tcatcattat gcacagcaca
    26521 accagatata agaaaaatac taattaatga tatagcagct atctttttca tctcaccttc
    26581 ccccattaaa taccaacgac actctctagt gtttaaatat aataatggca tgattattat
    26641 aattgaatag gattataata aatgttctgt acaacatttc ctacataagt aggaattacg
    26701 gacattgagg cccttcaggg taactccatg ggggctttaa tatattatat tgaagatgcc
    26761 actgtttagt tgaatattag gtatatgctc ttttttgaaa tttatcggtg gcagccgtta
    26821 gtattcgctg tccccattgc aagctcctgg tggtaaccac tgaatcctcc atacttgaac
    26881 tgacttttta tcctccgact ttcatcctgt tctgactcca ccttttgttt tctgctctac
    26941 actatctaca gaccaatcat aaaggcacat acgatcatgg cagaatttcc cgccagctta
    27001 ctgattctta atggtaaaag tactgacaat ctacccttgc gcgaagcaat tatgctgttg
    27061 cgtgaggaag gaatgacgat ccatgtgcgg gtcacctggg agaaaggtga tgccgcacga
    27121 tttgtagagg aggcccggaa gttgggcgtc gcaacggtga ttgccggtgg tggcgatggc
    27181 accattaatg aagtttctac ggcgttgatt cagtgtgagg gggatgacat acccgcgctg
    27241 ggaattttgc cattaggaac cgccaatgat tttgccacca gtgtagggat tcctgaggca
    27301 ctggataagg cgctgaaact ggcaattgcc ggtaatgcca ttgcgataga tatggcgcag
    27361 gtcaacaaac aaacctgttt tattaatatg gcgacaggcg gatttgggac gcgtattacc
    27421 acagaaacgc cggaaaaatt aaaagccgcg ctgggtggcg tctcttacat cattcatggc
    27481 ttaatgcgca tggatactct gcaaccggac cgttgtgaaa tccgcggtga aaactttcac
    27541 tggcaaggtg acgccctggt cattggtatt ggtaacgggc gtcaggccgg tggcggtcaa
    27601 caattgtgcc cgaacgcgtt aattaacgat ggcttgctgc aactgcgcat ttttaccggc
    27661 gatgaaattc ttccggctct cgtatcaacc ttaaaatctg acgaagataa cccgaatatt
    27721 atcgaaggcg cttcgtcgtg gtttgatata caagccccac acgaaatcac ttttaatctt
    27781 gatggcgaac cgttgagtgg gcaaaacttc catattgaaa tacttccggc ggcgttgcgt
    27841 tgtcgattac caccagattg tccattattg cgttaatcaa actattctct cttgaaccag
    27901 ggagaatccg taatgccgga tgcggtgtaa acaccttatc cggcatacag aacaatacta
    27961 caccgtaatt aattttaccc gtgtcttctg caatgccaat ttatccacct ctgacacagc
    28021 atcatcggta acaataatgt caattttttc gattggcagt acctgattaa aaccgcgacg
    28081 attaaatttc gatgaatcga gaacggcaat cactttattt gcggcggtga ccatcgcccc
    28141 gctaatcgaa taaccttcat taaaggtcgt aataccatta accgcatcaa taccatcagc
    28201 accgacaaac attaaatcgg cattaatatc ctgcaatgaa cgctcggcaa tggaaccatg
    28261 catcgagcgc gttttatggc ggaccgtgcc accacagacg accagagtaa tgtctttatt
    28321 ttcggaaagg gcaaacgctg ccgggagact gttggtaatc accgtgatat ttttagcggt
    28381 cattaatcct tcggcgataa gcattgtggt acttccgctg tcgagaataa cggtcatccc
    28441 ttcatggatc attttgactg ccgcctgcgc tattctgttt tttggcgcgc tggcgagctg
    28501 aaatcgctct ttaaacccga cttcctgggt ctcggtttca ctattaccgg acattatttt
    28561 cgccgcaccg ccatgaaagc gcgtaaccac gcctttttgt tcgagaaagc gcaaatcggc
    28621 acggattgtc gcttccgagg cagcaaatac tcccgccaga tcctgaacaa gcacggttcc
    28681 ctgttcattc actaattgga tgatctt
//
LOCUS       DAJTUP010000045        28366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTUP010000045 DAJTUP010000000
VERSION     DAJTUP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.045"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..654
                     /locus_tag="OO468_002013"
                     /pseudo
     CDS             1..654
                     /locus_tag="OO468_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            651..1037
                     /locus_tag="OO468_002014"
     CDS             651..1037
                     /locus_tag="OO468_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259135.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1062..3275
                     /gene="tssI"
                     /locus_tag="OO468_002015"
     CDS             1062..3275
                     /gene="tssI"
                     /locus_tag="OO468_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR6259136.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3298..3743
                     /locus_tag="OO468_002016"
                     /pseudo
     CDS             3298..3743
                     /locus_tag="OO468_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3765..4172
                     /locus_tag="OO468_002017"
     CDS             3765..4172
                     /locus_tag="OO468_002017"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR6259137.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4169..6457
                     /locus_tag="OO468_002018"
     CDS             4169..6457
                     /locus_tag="OO468_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR6259138.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6454..7443
                     /locus_tag="OO468_002019"
     CDS             6454..7443
                     /locus_tag="OO468_002019"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259139.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7543..7746
                     /locus_tag="OO468_002020"
     CDS             7543..7746
                     /locus_tag="OO468_002020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259140.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7764..8084
                     /locus_tag="OO468_002021"
     CDS             7764..8084
                     /locus_tag="OO468_002021"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259141.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8150..8382)
                     /locus_tag="OO468_002022"
                     /pseudo
     CDS             complement(8150..8382)
                     /locus_tag="OO468_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8492..9262)
                     /gene="yafV"
                     /locus_tag="OO468_002023"
     CDS             complement(8492..9262)
                     /gene="yafV"
                     /locus_tag="OO468_002023"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR6259142.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9443..9889
                     /gene="ivy"
                     /locus_tag="OO468_002024"
     CDS             9443..9889
                     /gene="ivy"
                     /locus_tag="OO468_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR6259143.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9932..12376)
                     /gene="fadE"
                     /locus_tag="OO468_002025"
     CDS             complement(9932..12376)
                     /gene="fadE"
                     /locus_tag="OO468_002025"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR6259144.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12616..13194
                     /gene="lpcA"
                     /locus_tag="OO468_002026"
     CDS             12616..13194
                     /gene="lpcA"
                     /locus_tag="OO468_002026"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR6259145.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13400..14167
                     /locus_tag="OO468_002027"
     CDS             13400..14167
                     /locus_tag="OO468_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR6259146.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14138..14878)
                     /locus_tag="OO468_002028"
     CDS             complement(14138..14878)
                     /locus_tag="OO468_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR6259147.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15262..15783
                     /locus_tag="OO468_002029"
                     /pseudo
     CDS             15262..15783
                     /locus_tag="OO468_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15959..16456
                     /gene="rayT"
                     /locus_tag="OO468_002030"
     CDS             15959..16456
                     /gene="rayT"
                     /locus_tag="OO468_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR6259148.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16673..18385)
                     /locus_tag="OO468_002031"
                     /pseudo
     CDS             complement(16673..18385)
                     /locus_tag="OO468_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18357..19142
                     /gene="lafU"
                     /locus_tag="OO468_002032"
     CDS             18357..19142
                     /gene="lafU"
                     /locus_tag="OO468_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR6259149.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19213..20268
                     /gene="dinB"
                     /locus_tag="OO468_002033"
     CDS             19213..20268
                     /gene="dinB"
                     /locus_tag="OO468_002033"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR6259150.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20320..20613
                     /gene="yafN"
                     /locus_tag="OO468_002034"
     CDS             20320..20613
                     /gene="yafN"
                     /locus_tag="OO468_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR6259151.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20616..21014
                     /gene="yafO"
                     /locus_tag="OO468_002035"
     CDS             20616..21014
                     /gene="yafO"
                     /locus_tag="OO468_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR6259152.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21024..21476
                     /locus_tag="OO468_002036"
     CDS             21024..21476
                     /locus_tag="OO468_002036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6259153.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21722..22765
                     /locus_tag="OO468_002037"
     CDS             21722..22765
                     /locus_tag="OO468_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6259154.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22827..23441
                     /locus_tag="OO468_002038"
                     /pseudo
     CDS             22827..23441
                     /locus_tag="OO468_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23498..24955)
                     /gene="pepD"
                     /locus_tag="OO468_002039"
     CDS             complement(23498..24955)
                     /gene="pepD"
                     /locus_tag="OO468_002039"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6259155.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25217..25675
                     /gene="gpt"
                     /locus_tag="OO468_002040"
     CDS             25217..25675
                     /gene="gpt"
                     /locus_tag="OO468_002040"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6259156.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25767..27011
                     /gene="frsA"
                     /locus_tag="OO468_002041"
     CDS             25767..27011
                     /gene="frsA"
                     /locus_tag="OO468_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6259157.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27069..27470
                     /gene="crl"
                     /locus_tag="OO468_002042"
     CDS             27069..27470
                     /gene="crl"
                     /locus_tag="OO468_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6259158.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27509..>28366)
                     /gene="phoE"
                     /locus_tag="OO468_002043"
     CDS             complement(27509..>28366)
                     /gene="phoE"
                     /locus_tag="OO468_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR6259159.1"
                     /translation="INDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGLKYKDLGSF
                     DYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNL
                     TLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGK
                     RAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSL
                     GYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDT
                     VAVGMTYQF"
BASE COUNT         6998 a         7057 c         7557 g         6754 t
ORIGIN      
        1 gaaaaaacaa atattgatgg caatatcatt atacataagg atggtaggag gacgtattta
       61 ttaaatcaaa atactggttc gattacggct gtgataagat ggaagtatga gtagtataag
      121 gataataccg ttacagatga ctggactata tgggaaaaag ctatttttat gcgagcgata
      181 tatttatctg ttcagcaggc atggaatggg aaaataactt attctgtttc cggtgaatct
      241 gagttcgcta aaaaatttca gggtaaagct ttgccttttg atgtaagaat aatttctgca
      301 tctcagaatg aagactggtt ggttattgca acaaaggttt tgcctggtgc tgatttaaga
      361 acatatgttg attttaaaaa cagtacggtt catgttgatt cagcgggcct ggaaaaagta
      421 gcgaaatgta ttaactgtaa taatacgttg caagtaaata tacctcatga agccggacat
      481 gtacttggtt acttagatga tgattatgat tcttcgtctc cgtatgttgg agatattagt
      541 gggttgatga atgtgggaat ggagctttgg gaacgatacc ttaaaaacgc aactataacg
      601 ttgaatatca taatgcctga aactaaattt actttattga atgtaaccaa atgaaatttt
      661 tctgtattat agtgattttc ctttcaataa attacatagc tatagtgaat gttttcaagt
      721 cttctgcagt tgctcaatcc accaaatatt atgaagaaaa tgatatacat gatggaagta
      781 atgacatctt ttttatgaag aaatatgggc cgcctgctca tgagttcgtg aggccacgat
      841 ataaatttgt tggtgagtat tatatcggct tgaatcaaac ttatcgcata acagatcctg
      901 aatttcaaga tgttcctatt aaggaaatgt tctggaattt gcagggggat ttgaatctga
      961 catgttggtt tcattataaa gacgggcaat ggagagtgat tacttatatt ttttggccac
     1021 caggggctag attttaatcc ggtattaaag gagcaaatat catgagtttt gtatctacgg
     1081 gtaataaacc agttggaaac ggtggacccg tcatcaccac accaccaata gctggcgaaa
     1141 gtggcggtat gagcacaggt tcagctgtaa ccgatgtatc tggtgccgcc gaggagatgg
     1201 cagaacaggc tgcggcggac ctgtttggtg cgctgccgga accgtcaggt ctggttaaag
     1261 ccgcagtagc tgctgcgcag gctgccgctg cagcagcagg gatctcggat atggctggtg
     1321 cagttcagga tgcagcggcg tcgcttgcag ccggtgctcc gggggcgcat aacgtcacag
     1381 taagtggtag tgcggtgccg ccgcagatgc ttttgtttgc aggcatgaat ggttcggaag
     1441 ggcttgggaa tctgttttct tacacagtac agcttaaaac gctggacgcc ctgaacctgg
     1501 ggtacgtttc ccccgctgct aacctgccgc ttcagctcat ggtgggcaaa gacctgtgcg
     1561 tcagtatcga actggacggc ggcggaaaac ggtatatcag cgggctggtg acggcggcga
     1621 gggtggtcgg ccatgagagc cgttcggtaa cgtatgaatt acgcattgag ccatggctga
     1681 agctgctgac ccataccagc gactacaaag cgttccagaa caaaaccgtg gtggatattc
     1741 tcgatgaagt actggatgaa tattcctttc cggtggaaaa gcggctggtg gaaaattatc
     1801 caccccgcgc ctggcaggtg cagtatggtg aaaccgactt cgatttcatc cagcgcctga
     1861 tgcaggaatg ggggatttac tggtggtttg agcacagcga gaacagccac acgctggtgc
     1921 tggtggatgc catcaatggt cataaagcgt gccctgactc gccgctggtg gagtggcacc
     1981 aggaagggct gaaactggat aaggaattta tccacaccat tacggccagt gagaggctgc
     2041 gtactggaaa gtgggtgatg gacgattttg attttatgaa gcctcgttcg ctgcttaaaa
     2101 gtaccgtggc cagtccgcgt gataccggtc atgctgaata cgagcattac gaatggcctg
     2161 gtgattactt cactacgggc gaaggcgaga tgctgacgcg cattcgtatg gaagcgcagc
     2221 gcagcccagg cagccgggca catggggccg gtcatatccg taccctcatg accgggtaca
     2281 cctttacgtt gatgaatcac ccgacggcag aaattaatca ggaatatctg ctggtacaga
     2341 ccactctgtt tttacgggat aatgcgcagc acagcggtca ggatcagcat tttacctatg
     2401 tcaccacgtt tgaactgcac cccaccagcg aagtttaccg cccacaacga accctgagca
     2461 aaccccacac caaaggcccg cagagcgcca tcgtcaccgg cccggcgggg caggaaatct
     2521 ggacggataa atatggccgg gtgaaggtgc agtttggctg ggaccgttac ggaaagaatg
     2581 acgaaaacag ttcctgctgg atccgcgtca gctatccgtg ggcgggtaaa ggattcggca
     2641 tgatccagat cccgcgtatt ggccaggaag tactggtgga tttcaaaaac ggcgatccgg
     2701 atttgcccat catcgtgggg cgcacctata accaggacac catgccgccg tgggggctgc
     2761 cgggcatggc gtcgcagagt gggatattca gccactcatt gcagggaggg ccgactaacg
     2821 gaaatatgct gcgcttcgat gataaaacgg gtgcggaaga ggtgaagttc catgcggaaa
     2881 aagacctcaa taccacggta aaaaacaatg aaacccacac ggtcatggtc gatcgcataa
     2941 aaaccattgt caaaaatgaa accatcagcg tgggagaaga ccgtaatgcc accatcacaa
     3001 aaaatgatgg tctttccgtc aaactggctc aaaccatcaa tgtcggaacg acatatcgtc
     3061 tggatgttgg cgatcaattt acccttcgtt gcggcaatgc agcgcttgta ttacacaagg
     3121 atggctcgat tgagttttgc ggaaaacaac tgttactgca caccagcgat gttatgcaac
     3181 tcattggcag tggcattgat atgaatccgg atggcggcac agccatgact gcggatgata
     3241 ttgccccgtt acctaccccc gacgtttctg aataaaaaat ataaaaggaa tttttctatg
     3301 accagccttt accgtattca ggagggctgc tttgccctgc ctgaaacttt tcttgatcgc
     3361 accgtcaata tcttcgtccc atcgggcaat gaaagggcaa ctcccagtct gaatattttc
     3421 cgcgatacgc tcaggccaga tgaaaatctg accacctata ttgaccgcca gatagccttg
     3481 atgaaaaaaa aaacctaggc cagcaccggg tactttcgcg gggaacggca ctggcgggaa
     3541 ctggcgaagc tgccatgaca ggtgaacaga ttctcgcaac tcatcgttct ggaaaaacag
     3601 aagtgtacca gcgtcaggcc gggtttatca ccgggccagc aaaggtacta atgttgacgc
     3661 tgaccactca gcgtcctttt gatgatcaca ccgaccagct atggactgca tggttgacta
     3721 gttttcagcc tgctaaaagc taaaaaaaac agggaggttt tgtcatgtat gaagccgcac
     3781 gggtggatga ccccatctac cacaccagcg cccttgccgg gtttttgata ggggccatca
     3841 taggtatcgc cattattgcc gtcgccagct ttgctttttt tacctgcggc tttttggccg
     3901 ggctgatttt gagttttctg gctgatcaga tagcttccgg agtcctgcaa ctgggtgagg
     3961 ctattgggcg ctcgatacat tccacagtgg gaaaaattct gaccggttct gataatgtca
     4021 gcaccaacag tcgcccggcc gcccgggccg tactgagtac ggtgatatgc gaagaccatt
     4081 ccccggaaat ccgcattgcc caaggttccg gcaacatcta catcaacagc cagctgcccg
     4141 taaggatgac cacactgagt gtgatgcagt gattgaggac ggctccccca atgtctttct
     4201 cggcggcggt acgcagacgg tactggaaat cagcccggaa atccctgact ggctgcgtca
     4261 ggtggtggat gtgctgtatg tggtcgccgg aatgcttggc gggctggctg gcgcctggcg
     4321 tcaggcggca aagatggggt cgaaatttgg tacaaaatgt gcggcaaaat ttattggtgg
     4381 cgaaatggtc gggatggcca tctctgacac agtcatggga ttgttcagca atccggtaga
     4441 tgtcaccact gggcaaaaaa ttttgctgcc ggagacggat tttaccctgc caggacgcct
     4501 gcctgtcacc tgttcgcgct tttacgccag ccacctggaa actgaaggat tgctgggcag
     4561 aggatggcgg ctgaactggg agataaacct gcgtgaggat gaaacatata tcaccttcat
     4621 cggtgtacag ggtcgggaac tgagttatcc gaaagagatg ctgataccgg gccaccagat
     4681 attcgacccg gaagagcagt tttacctgag ccgtctgcat gacggatgct atgtgctgca
     4741 ttacaccgac tgcagctatt acgtgtttga tgaatttgat gaccacggcg tcgcgccgtt
     4801 actttttatg gagacgccgt accggcagcg tattgctttc gggcgtgaga acggcaggct
     4861 ggtgcgggtg gcctccagca gcggacacca tctgttattg caccgcacga tgacgcaagc
     4921 aggggaacgc ctgtcgcata ttgaactttt gaagggcggg cgtcccggta acctggtgga
     4981 atatcgttac gacgataacg ggcaactgac tggcgtggtg aaccgggctg gtgtgacggt
     5041 gcgccagttt gcctatgaaa atggcctgat gacagagcat cgcaacgcga cgggctttac
     5101 ctgtacctac cactgggaag agattgaagg tttcccgcgc gtggtggaac acacgaccag
     5161 cgacggcgag gattaccggt tccactatga ttttgctggc gggcagacgg tggtaaccgg
     5221 caggccggag cagaagtggc agtggtggtt tgatgaagag acgtatgtga ctgcgcaccg
     5281 gacgccgggc ggcggaatgt accgcttcac gtacaacgaa aaccattttc cggtagcggt
     5341 ggaactcccc ggcgaacgca gggtaacgct ggaatacgac accctgagcc gggtggtgaa
     5401 agagacggac ccggcaggcc gggtgacaca gacgcagtgg aacggcagtt ttgctgaaat
     5461 cacgcgccgg gcgctggatg acgaccacgt atggaaggcg gattacaacg aacacggcca
     5521 ggtgatacgg gagacggacc cggaagggcg cgtcacgcgg tacgggtatg atgatcaggg
     5581 gctgccggag acggtctgcc accccggtaa acagcaggac cgttacacct ggaacgcgct
     5641 ggggttactg agcagccacc gtcgcatcac gggcagtgtg cagtcctggc agtatacgca
     5701 gcgcgggatg ctggcgcgcc atacggatga agagaaacgg gagacgcgct ggcagtacac
     5761 cccggaaggg ctggttgcca gtctgagcaa cggcaacggg gctcagtacc ggttcagcta
     5821 cgacggcgac ggacggctga cgggagaaca gcgcccggac ggcctgataa ggatgtttgc
     5881 gctgaatgcg gacgggtttc cggtcatcat cccgacgcag ggcacagagg gcggggtgcg
     5941 caatgaacag caggagcgtg atgcgctggg ccgcctgctg cgcagtgaca cgcagcacag
     6001 tacacggacc ttcagctaca accgtcttga ccagataacg gaagtgacgc tcacccccac
     6061 ggaagaaggg gaacggctgc accacatgca ggcggacacg gtgcgttttg cgtatgaccg
     6121 cagcgggtgg ctgacggcag agcacagcgt gcacggcagt atcaaatacc ggcgcgatgc
     6181 gctgggcaat cccaccgata tcacgttgcc ggacgggcag catctcagtc atctttacta
     6241 cggcagcggg catctgctcc agacggcact ggacggtatc acggtcagtg aatatgagcg
     6301 tgacagcctg caccgtcagg tgatacgcac gcaggggaaa ctggcgacgt tcagcggcta
     6361 caacgccgac aaccggttaa gctggcagcg cagcctgcca ggtggcagcc agaatccaca
     6421 gcaggcggta ctcccccggc gcagaacgac agcgtgacgg cgcgggacta tatctggaac
     6481 gcagacggtg aagtcggcgg catcaatgac aaactgcgcg gttgcctggt gttcagctat
     6541 gaccgcagcg gctggctgac cagccgcacg gggcagatgt atgaccatga ccattattat
     6601 tacgacaaag cgggcaacct gctgacggat gagtatcagg gagcggtgat ggataaccgc
     6661 ctgccgggtt acgggcgtga ccgctaccgc tataacgaat ggggcgaact gacagagcgc
     6721 agggaccagc agctggagtg gaacgcgcag ggccagttaa cgcgggtcat cagcagcaac
     6781 agcgagacgc ggtatcagta cgatgcgctg ggcaggcgca tcagcaaggc gaccagcaat
     6841 ctccataccg accggggaga acgaagccgg acaacgtttg tgtgggaagg gttccggctg
     6901 ttacaggaga caacgtggca gggaaagcgg acatacctgt acgatgcgga acagccgtac
     6961 acgccggtgg ctgccatcac ggggcgcgga gagagccaga aaatctggta ttaccacacg
     7021 gacctgacgg gcacggtaca cgaggtaacc gcgccggacg ggacgctggt gtgggcgggc
     7081 taccaggcgg gatttggtga aaaccggggc gacatcagca acagcggggc gtactttgag
     7141 cagccgcagc ggctgccggg gcagtacttt gacgaagaaa cagggctgca ttacaatctg
     7201 ttcaggtatt atgcgccgga gtgcgggagg ttcgtaagcc aggacccgat aggattaaac
     7261 ggtgggttga acttatatgc ttatgcgcct aatccgttag gttggatcga tccgctagga
     7321 ctgaattctt taggaataga ttcaaataat atatttagag gagatggtaa ctataaaggt
     7381 ggcggaattg gacagccaaa gagtggcaca atatcagcac aggacattat tgatcatctc
     7441 taagaaaaag gtgatgggcg tttaacatct tttagtacta aaaaaacatc tagttccaac
     7501 tctcgtggtg cagatttttt ctcaaaaaaa catgtcttca aagtggcagt ttctgatctc
     7561 gatgatttag ttaaaaataa tcagatcact gttttaacac aagaaaaagt aaaagcaata
     7621 tttggtttag aatcaagcaa gaaaattaga aaagaacttg gtaatattct tcagaatatg
     7681 gctaggaata atgaggtgtt agttcatggg caaattcttg gaaataaagt tagccgatgt
     7741 aattagttag tggagaggaa gttgtggacg aaattagaat aagaattgct ggtaatacgg
     7801 ctgcgccttg tttccatgtg ctaaaaaata agggatataa agtaacaatg gaaactcatg
     7861 cattatctga cgattgttta gattgtgtat atgattggta tgcggaaaaa aatggacaat
     7921 tttttagtgc aacgagtcca gaagagttac ttggattagt tgcaatgtgg gaagttcgtg
     7981 gtaatgactg gagagctaat ttgaatgaga taaatactta tcaaaaagcc atagaagaat
     8041 ccccgttttt tgataaaaac ggcaatgaaa tagagtctga ctaaatttta ttgatatagc
     8101 aacgataata accggaaatt tcttccagat ctttccggct ttttattatt taactttcgt
     8161 tcagcacact aaacatcccg tctcgcatcc ccttaactgg ctgtttaacc tgataaagtt
     8221 ccctgcgcag ttcctgtact tcgttgttat cccaagcagg atcaaacatc cctccgagat
     8281 cttcacggta acgcccgtgc cgatctcaaa tcccgctttt ctcagccatt tggcgctgat
     8341 tttgatcacc ggcgtgtcgc tgcggttggg cagataaccc acggtacagc gacgctgtgt
     8401 tttggaaatg gtgacttctg gcgtaatatt gtgtttaagt taggggcttt cgtaatctag
     8461 cccagccccc ggccctaaac acaaccccca ctcaccacaa cctaaactca tccgcatccc
     8521 gccatgccgg aaacttttct ctatattccc gcaatgccac catcgacagc tccgcatcaa
     8581 tacgcgttgc cttatgcgcg tcggcggtag cgataatctc gccttgcgga ttaatcaccc
     8641 ggctgtcacc gcgataatgg cagccgttgc catcgctgcc gacgcgattg catcccgcca
     8701 catacgcctg attctcaatc gcgcgggccg tcagcaatgc ctgccagtgc agagagcgcg
     8761 gagctggcca gttggcgacg tacagggcga ggtcataatc gttgagattg cgcgaccaca
     8821 ctggaaaacg taagtcgtag cacaccagcg gcaaaatacg ccagccgcgc cattccacaa
     8881 tcactcgcgc attgcccgct ttataatgca gatgctcatc tgccatgcgg aacagatgac
     8941 gcttatcata aaaatgtacc gtgccgcccg gctcaaccag caaaaagcgg ttaaccgaac
     9001 cagactccgt ttgtaatgca acactgcctg caatcagcgc attgcactgc tgcgccttag
     9061 ctgtcatcca gttcactacg tcatcttgtg caagcgacga agctgccgct tccatggcaa
     9121 agccgctggt aaacatctcc ggtagaacga tcacatcgcg cccggtaata ccttccagtt
     9181 gacgatcaaa atggcgcagg ttggcaggac catccaccca caccaacggt tgctgcaaaa
     9241 gcgtaatctt caaactaggc acggtgtaca actcctttat gcgaagggtt ttataacttt
     9301 aacaccttat caggcagttg ccttagcgca gaataaattg ataacaaatg ctgatattgg
     9361 aaatatctga tttgcaaatt atcgtgttat cgctaggctt taggaggtta ataacatggg
     9421 caggataagc tcgggaggaa tgatgtttaa ggcaataacg acagtcgccg cactggtcat
     9481 cgccaccagt gcaatggcgc aggatgattt aaccattagc agccttgcaa agggcgaaac
     9541 caccaaagct gcgtttaatc agatggtgca agggcataag ctacctgcat gggtgatgaa
     9601 aggcggtact tatactcccg cacaaaccgt aacgttggga gatgagacgt atcaggtgat
     9661 gagcgcgtgc aaaccgcatg actgtggctc gcaacgtatc gctgtgatgt ggtccgagaa
     9721 atctaatcag atgacggggc tgttctcggc tattgatgag aaaacgtcgc aagagaaact
     9781 cacctggctg aatgtgaacg atgcgctttc gattgatggt aaaacggtgc tgttcgcggc
     9841 gttgaccggc agcctggaaa accatccgga tggctttaat tttaaataat tagcggataa
     9901 agaaacggag cctttcggct ccgttattca attacgcggc ttcaactttc cgcactttct
     9961 ccggcaactt taccggcttc gtcgccagct cttccggatc aaagtcatca acgttaatac
    10021 tgcgcagacg gctttcttcc gctttcacca gaatagcggc ttcatcttta tcaatcagcc
    10081 ccttcgtcag cgcgttgtgc gccagttcat ccagacgggt aaatggcaga tttttaccca
    10141 gctctttaca gatccgctga tgaatcgggt cggcggcaat cacatccacc aacgcctctt
    10201 ccagtaaacc aaccgggtta tgctcgcttg gcgtcaggta ctgaccgcga ccaatacgtg
    10261 aacgagtggc gttcggcact tgtaaaatct tcgccatttt atgatccagc ttgtcagacg
    10321 gtgccagata atgacgtccg gtcgggaaga tcaccacatt cagcagcccg gcaaccacgc
    10381 ggttcgggaa gttttgcagt aaattgtcca ttgcctgttc tgcctgatac agcgcatcct
    10441 gtacgcccca gtgcaccagc ggcagatcgg cttcattacg gccttcgtcg tcataacgct
    10501 tcagaacggc agaggcgagg taaagctggc ttaaaatatc ccccagacgt gccgagatac
    10561 gctcacgacg tttcaggctg ccgcccagca gtgccatcga gacatcagaa agcagggcga
    10621 ggttggcgct caggcggttc aggtgctgat agtagcgttt agtggcatcg ccggttggcg
    10681 tgctgctggt taaaccgcgc gtcaggccca gccagaagct gcgaactttg ttgctaccga
    10741 cgtgaccgat atgtttgaac aacagtttat cgaacgcgtt gacgtcattg ttcttcgccg
    10801 cttccatctc ttccagcacg tacggatggc aacgaatcgc tccttgtccg aagatcatca
    10861 tgctgcgggt cagaatgtta gccccttcaa cggtgatggc aatcggtgcg ccctggtaag
    10921 cacgcgccag gaagttgctt tgcccgagca taatgccttt accgccggta atatccatcg
    10981 catcaataat cgactgctgc ccgcggtggg tacagtgata cttaacgata gccgacagca
    11041 cggcaggttt ttcgccgagc ataatgccgt aggtaatcag cgatgccgca gcatccatta
    11101 cataagcgtt cccggcaata cgcgccagcg gctcttcaat cccttccatc ttaccgatag
    11161 agattttgaa ctgacggcga atgtgagaat acgcgcccgt tgccagcgct accgatttca
    11221 cgccgccggt tgagttggaa ggcagggtga tgccgcggcc taccgagagg cactccacca
    11281 gcatccgcca gccttgcccg gccattttcg gcccgccgat gatgtaatcg atcggcacga
    11341 agacatcttt accgcgcgtc ggtccgttct ggaacggtac gttcagcggg aagtggcgac
    11401 gaccaatttc cacgcccggc gtggtggttg ggatcagcgc acaggtaatg cctaaatctt
    11461 ctgcaccgcc gagtaatttt tccgggtcgg agagtttaaa cgccagccca agcacggtcg
    11521 caatcggtgc cagcgtaatg tagcgtttgt tccaggtcag acgcatcccc agcacctgct
    11581 ggccctgcca ttcgcccatg cagacaatcc cggtgtccgg aatcgcgccc gcatcggaac
    11641 ccgcttccgg gctggtcagt gcaaagcagg ggatctcctg accacgcgcc agacgcggca
    11701 gatagtgatc tttctgctcg tcagtgccgt aatgttgcaa cagttcgccc gggcctaatg
    11761 agtttggcac gccgacggta atcgccagga tcccgctcac gccagagagt ttttgcagca
    11821 cacgagactg ggcataagct gagaactcca gtccgccgta ctcttttttg ataatcatcg
    11881 cgaagaagcg atgctcttta aggtacgccc acaactccgg tggcagatcc gccagctcat
    11941 gagtaatctg gaaatcattc gccatccggc aggcttcttc taccgggccg tcgagaaacg
    12001 cttgctcttc agcggtcagg cgcggctgcg gatagttatg cagctttttc cagtccggct
    12061 tgccctggaa caagtcgccc tcccaccagg tggtgcccgc atcaatcgct tctttctcag
    12121 tgcgcgacat cggcggcatc accttacgga aaccgcgaaa taccggcgcg gaaatcatcg
    12181 acttacgcat aggcgcaaag ttaaatggca cgaggataat ggtcagaggc accagtaccc
    12241 acgccgacca cagaccagca acgccgaggg cggctgtcca ggcgagcaaa atcagactgc
    12301 tgataaataa gctcacgcgg tgatagaaca acgcgccgag caggacaacc gtagcgagaa
    12361 tactcaaaat catcataacg aaaagcccct tacttgtagg aggtctgacc acttgtgatg
    12421 atatggttgt agtggatgta aaaacattta gcaatgtgtt tacaatataa ttacaacaaa
    12481 gctcacattg ttgctgtttt tatccgcact tcaggtcaaa aagtcctggt catagcacct
    12541 gcccgtactt ctcgcttttg gcggtatccg gtacactgca ttttgtctat tacatttatg
    12601 ctgaaggata tcctcatgta ccaggatctt attcgtaacg aactgaacga agcggcggaa
    12661 acgctggcta actttttaaa agatgacgcc aatattcacg ccattcagcg cgcggcggtc
    12721 ctgttagcag acagctttaa agccggtggc aaagtgcttt cctgcggcaa cggcggttcc
    12781 cattgcgacg ctatgcactt tgccgaagag ttgaccggtc gctaccgtga aaaccgtccg
    12841 ggctacccgg cgattgctat ttctgacgtt agtcatattt cctgcgtcgg taatgatttc
    12901 ggtttcaatg atattttctc ccgctacgtt gaagcggtag gtcgcgaagg cgatgtactg
    12961 ctggggatct ccacctccgg taactctgca aacgtgatca aagcgatcgc agcggcgcgt
    13021 gagaagggga tgaaagtgat caccctgacc ggtaaagacg gcggtaaaat ggctggcacg
    13081 gcggatatcg aaattcgcgt tccgcacttt ggttatgccg accgcattca ggagattcac
    13141 attaaagtga tccatatcct gatccagtta attgaaaaag agatggttaa gtaagtctgg
    13201 cgtaggccgg ataaggcgtt tacgccgcat ccggcacttg tgcgctgatg cctgatgcga
    13261 tgctgacgcg tcttatcatg cctacaaatc tgtacccgaa ccgtaggccg aataattcgt
    13321 ttacgccgca tccgacctga aaattcttaa atcaatcttc gccggggtcc atgcgctccc
    13381 gctgttgtgg aggttaccca tgtgcgaatt gctcgggatg agcgccaacg tccctaccga
    13441 tatctgcttt agtttcaccg gacttgtaca gcgtggtggt ggaaccgggc cacataaaga
    13501 tggctggggc attacctttt acgaaggtaa aggctgtcgc acatttaaag atccacaacc
    13561 cagctttaat tcccccatcg ccaaacttgt ccaggactac ccgataaaat cctgttcggt
    13621 ggtggctcat attcgccagg ctaatcgggg cgaggtggcg ctggaaaata ctcacccatt
    13681 tacccgcgag ttatgggggc gtaactggac ttatgcccat aacggacaac tgacgggcta
    13741 caaatcactg gaaaccggca acttccgccc ggtcggtgaa accgacagcg aaaaagcctt
    13801 ttgctggctg ctgcataaat taacgcagcg ttacccgcgc acgccgggca acatggcggc
    13861 agtgtttaaa tatatcgcct cactggcgga tgaactgcgg gcgaagggcg ttttcaacat
    13921 gctgctttcg gacgggcgct atgtaatggc gtattgctcg actaatttac actggatcac
    13981 ccgccgcgcg ccgtttggcg tggcaacgtt gctggatcag gatgtggaaa tcgacttcag
    14041 ctcgcagacc acaccgaatg atgtggtcac ggtgattgcg acacagccgc tgacgggcaa
    14101 tgaaacctgg caaaaaatta tgccaggcga atggcgctta ttttgcctcg gggagcgtgt
    14161 agtttgatgc cagttgtggc tgcaccactt cgtggcttaa cggcttgctg accacgtaac
    14221 ggccattggc cacagaaacg gtcggtggct tacgggtttg ctcaaagtag tcgtagcccg
    14281 gcttcagctg ctcccagaag tccttaaagt tggaatattt atggcgcttc atattggcgt
    14341 cggtcatgcg gaatgggtaa atactcacct gcacgctcgg ctgaccaaac accagcgcac
    14401 cagtaacgaa ctggaagatc tcatcaatac cctgattggt cattgcgtag cagccgatgg
    14461 atacacaatc gccgtggatc atcaggtatt tcccttcata accatgcgca cggtcataag
    14521 cattggggaa tccaatatta atcgctttgt agtaacggct gtctggtttt aactgattac
    14581 gctggacgct ataaaaccct tccgggcttt tgaaatcgcc ctgacgctgt tttggcccta
    14641 agccgccgga atatttacag attttataac tgtcgagcag ttgatattgc tcgcccattt
    14701 tgacgtaaag atcgagcgta cgttcttcct tgaagatctg gatgtagaca ggggatccca
    14761 tcaactgctg cttatactct ttgctcactg gcgtggtcga gctactgctg cccagcaaac
    14821 cggcaaacga aacgcacggg atcaacaaca tcgcaagaat taatgcgatt ttacgcatac
    14881 tgcttattcc ttgataaaac ggttacacac gccaggacgg caaaatggat cccaaatcgg
    14941 aatagtctgg atttggaagg ctcacattat caccaaaaga gttttacgca agcctgtcgg
    15001 cgctgggttt acaaatttag cacaggggca gttttcaata gtccgaaaag cgcatgtatc
    15061 atttatttcc taatattatg aaatctcaat agtaatatgc aaataagcaa ttaataatcc
    15121 cgtttactcc tccggtcttt taaaattctc accttcattc agccggttta tgtctttcat
    15181 gtcttcgttc ttgctcggca gatttttgca tccaggcgtg ttttcgttat gcgttttgct
    15241 ccctttactc gccagcgcca ccacaaccgg gagcagataa aacagcatcc agaatggttt
    15301 cctgcgccgc tcaaggcgag tgacagacgc tggcaggcgc tggcggaaaa caaccacttt
    15361 ttaagcagcg atcacctgca taacatcacc gaagtggcga ttcaccgcct ggagcagcag
    15421 cttggcaagc cttacgtctg gggcggcacg cggcccgatc aaggcttcga ttgtagtggg
    15481 ttagtttttt atgcctacaa caagatcctt gaggcgaaac tcccgcgcac ggccaatgag
    15541 atgtaccact accgtcgggc aacgattgtg gcgaataacg acctgcgccg gggagatttg
    15601 ctatttttcc atatccacag ccgcgagatt gccgaccata tgggggtgta tctgggcgac
    15661 ggtcagttta ttgagtcact gcgcaccggg gaaaccattc gggtaagccg gttagccgaa
    15721 cctttctggc aggaccattt tttaggcgcg cgcaggattt tgacggaaga gatgattttg
    15781 taggccggat aaggcgttta cgccgcatcc ggcggttgtg cacggatgcc tgatgcgacg
    15841 ctgacgcgtc ttatcatgcc tacaaatctg tgcgtttttg cgagccgctt tcccgatata
    15901 acacagcgtc attgtcggcc tttccttttc ctcttaccgt tgagaaaaag gagtcgctat
    15961 gtcggaatat cgtcgttatt acatcaaggg gggaacatgg tttttcacgg taaatttacg
    16021 caatcgtcga agccatcttc tgaccaccca gttccagacg ctccgtaacg ccattattaa
    16081 cgttaagcga gacagacctt ttgaaattaa cgcctgggtc gttttgccgg agcatatgca
    16141 ttgcatatgg acattacccg aaagcgatga tgatttttcc tctcgctggc gggagattaa
    16201 aaagcagttc acccatgctt gcggattgaa aaatatctgg caaccgcgtt tttgggagca
    16261 tgccattcgc aatacaaaag attatcgcca tcatgttgat tatatttata taaatccaga
    16321 gaaacatggc tgggtaaagc aggtgagtga ttggccattc tcaacgttcc atcgcgatgt
    16381 cgcgcgaggg ttatatccca tcgattgggc gggagacata acggatttaa gtgccgggga
    16441 gcgtatcatt ttataactgt gcgctggtgc cggatgcgct tacgcttatc cggcctacaa
    16501 atcgtgcttt aacggtaggc cggataaggc gttcacgccg catccggcag ttatgcgcag
    16561 atgcctgatg cgacgctggc gcgtcttatc atgcctaaaa acttgtgcct gaacggtagg
    16621 ccggataagg cgtttacgcc gcatccggca attgtgcacc gatgccggat tcttacatca
    16681 acgcccccat aatcttcaac tccagctcat ccggcacttc gttatacgac agcacatgca
    16741 gccccggcgc aaacaacctt gcataacgcg gcagcaaagg gcgtagctgc ggcggcacca
    16801 gcagcaccgg atctttcccc gccgctttca tctgctcttt cacctgtggc atcgtgctct
    16861 ggaactggtt gagcatattc gggtcaaccg gcacgctgtc gagcatcact ttgccggcct
    16921 gttgcgcctg attcactacg ttggtcagca gattttccag ctcattattc agcgtataca
    16981 ccgtcagctc ctgcttgcga acgaacggat gagtaatgct gcgccgcagc gccaggcgca
    17041 catcggccgc cagcagaata tgatctttag tcaccgtgct actggcgacc agcacggtgg
    17101 cgatggtgac gatatcgcgc agggaaacgc cttcggtcag cagcgcccgg tacactttca
    17161 gcaattggct gtaattgagc gccgcgctta aatcctccgc caggcgcggt gccatcgacg
    17221 acaaacggtt atgcaactgc gtgatgtcat catagttaaa caaatcagga atatagctgc
    17281 gcacaatctt gttcacatgg gtggcaatca cactggcgct gtcgatcacc tgatacccca
    17341 tattcagagc cttcgctttc tgcgcaggct ggatccaggt gaccggtatc ccgtacgccg
    17401 ggtcgttccc cagcacgccg tcaatctcgc cgtaggtttc gctggagggc agcgccatca
    17461 gtttatccgc cggaatatcc gcttcatcag cctcaatgcc gttgatgaaa atagcgtact
    17521 ggctgggctt gaggcggaag ttttcccgaa tgcggatctc cggcagcagc acgccgttac
    17581 cgtcggaaat cacctgccga acgccgcgaa tccgctgggt gagcgggttg ccctgcgctt
    17641 tgtccaccag cgccaccagc ttgtagccga ggcttaagct gatgggctcg atcagcggaa
    17701 tagtttccca actgacctgt tgttcgctgg tttcagtgat ggtgcgggtc agcgtttcga
    17761 ggcttttctc ttccgcctcc gccgcctgcg ggcgtttgct catccgccag ccggtaaagc
    17821 caagcagggc gctgaacagc aaaaacggca gatgcggcat ccccggcacc accgccagca
    17881 cgaacatgat cccggtggcg gtgtagagca ccgacgggct tgccagcaac tggtggcgca
    17941 cgtcatgggc gatatcgccg ttgtcgctga cgcgggtgac gataatcgcc gccgcggtgg
    18001 agagcagcag ggaagggatc tgcgccacca ggccgtcgcc gatggtcatc agcacgtatt
    18061 gctggaaggc ggcgtcggcg ctcaggttgt atttgaagat gccgatacag acgccgccgc
    18121 tcaggttgat cgccagaatc atcatcccgg cgatggcgtc tccgcgtaca aacttcgatg
    18181 ccccgtccat cgcgccgtag aaatcggcct cgctggcaac atctttacgc cgggtttgcg
    18241 cctgcgcctg gttgatcaat ccggcgttaa gatcggcgtc aatcgccatc tgtttgccgg
    18301 gcatcgcgtc cagggtaaag cgggcagaaa cctcggaaat acgctcggcc tctttggtga
    18361 cgacgataaa gttgattgtc aacagcgtca gtaaatccga gcgtgaaagc attatcgccg
    18421 cgctgcacgg gcagtcgatt tttaacggtg gtgggctgtc gccgttaaat aaaatcagcc
    18481 cgtctcatcc gccaaaacct gccaccgtgg cggtgccgga agagacggag aaaaaagcgc
    18541 gtgatgtgaa tgagaaaacg gcgctgctga agaagaaatc cgccaccgag cttggcgagt
    18601 tggcaaccag tatcaacacc attgcccgtg atgcgcatat ggaagcgaat ctggagatgg
    18661 agattgttcc ccagggatta cgcgtgctga ttaaagacga ccagaatcgc aatatgttcg
    18721 aacgcggcag cgcgcagatt atgccgttct ttaaaacttt gctggtggag ctcgcgccag
    18781 tgttcgactc gctctataac aaaattatta ttaccgggca taccgatgcg atggcctaca
    18841 aaaacaatat ctacaacaac tggaaccttt cgggcgatcg cgcgctttca gctcgtcggg
    18901 tgctggaaga ggccggaatg ccggaagata aagtgatgca ggtaagcgca atggcggacc
    18961 agatgctgct ggacgcgaag aatccgcaaa gcgcgggcaa tcggcgtatt gagattatgg
    19021 tgctgaccaa aagtgcgtcc gatacgctgt atcaatactt tggtcagcat ggggataaag
    19081 tggtgcagcc gctggtgcaa aagctggata agcagcaggt gctttcgcag cgaatgcgct
    19141 aaatgctgaa tctttacgca tttctcaaaa cctgaaatca ctgtatactt taccagtgtt
    19201 gagaggtgag caatgcgtaa aatcattcat gtggatatgg actgcttttt cgcagcggtg
    19261 gagatgcgcg acaatcccgc tctgcgcgat atccctattg ctattggcgg cagccgcgaa
    19321 cgtcgggggg tgatcagcac cgccaattat cccgcgcgta aatttggcgt acgtagcgct
    19381 atgcctactg ggatggcgct caaattatgc ccacatctca ccttgcttcc ggggcgcttt
    19441 gacgcctaca aagaagcctc aaatcatatc cgtgaaatct tctcgcgcta cacctcgcgc
    19501 attgaaccgt tgtcactgga tgaggcttat ctcgatgtca ccgatagcgt ccattgccac
    19561 ggttctgcga ccctcatcgc ccaggaaatc cgccagacga tcttcaacga gctgcaactg
    19621 acggcatctg cgggcgtggc accagtaaag tttctcgcca aaatcgcctc cgacatgaat
    19681 aaacccaacg gtcagtttgt gattacgccg gcagaagttc cggcattttt acaaacctta
    19741 ccactggaaa aaatccccgg cgtcggcaaa gtctcggcgg caaaactgga agcgatgggg
    19801 ctgcggacct gcggtgatgt acaaaagtgt gatctggtga ttctgcttaa acgctttggc
    19861 aaatttggcc gcattttgtg ggagcgtagt caggggattg acgagcgcga cgttaacagc
    19921 gaacggttac gaaaatccgt cggcgtggaa cgcacgatgg cggaagatat tcatcactgg
    19981 tctgaatgtg aagcgattat cgagcggctg tatccggaac ttgaacgccg tctggcaaag
    20041 gtaaaacctg atttactgat tgctcgccag ggggtgaaat taaagttcga cgattttcag
    20101 cagaccaccc aggagcacgt ctggccgcgg ctgaataaag ctgacttaat cgccaccgcg
    20161 cgtaaaacct gggatgaacg ccgcggcggg cgcggtgtgc gtctggtggg gctgcatgtg
    20221 acgttgcttg acccgcaaat ggaaagacaa ctggtgctgg gattatgatg tatactatta
    20281 tgtatattct ggtgtgcatt attatgaggg tatcactgta tgcatcgaat tctcgctgaa
    20341 aaatcggtca atatcactga gttacgtaaa aacccagcta aatactttat tgatcaacca
    20401 gttgcggttc tttctaataa tcgccccgca ggatatctct taagtgccag cgcattcgaa
    20461 gcgttaatgg acatgcttgc tgaacaagag gagaaaaagc ccataaaggc gcgctttcgt
    20521 ccaagtgctg caagattaga ggaaattaca cgccgcgcta aacaatatct taatgatatg
    20581 acggatgatg atttcaatga ctttaaggaa taaggatgcg ggtattcaaa acaaaactta
    20641 ttcgcctgca acttacagca gaggaacttg atgcgttaac ggcggatttt atttcctata
    20701 agcgtgacgg tgttttgcca gatatatttg gtcgcgatgc actctacgac gactcgttta
    20761 cctggccatt aatcaaattt gagcgagttg ctcatattca tctggcaaat gtgaataatc
    20821 catttccgcc acagttgcgc caattcagca gaacgaatga cgaagcgcat ttggtatatt
    20881 gtcagggtgc gtttgatgag caagcatggt tgctcattgc cattctgaag cctgaacctc
    20941 ataaactggc tcgagataac aaccaaatgc ataaaattgg aaaaatggca gaagcgtttc
    21001 gcatgcgttt ttgaatttat attatgaata acatacaaat aagagattat cagcctggcg
    21061 attttcagca actatgcgct attttcatta gagcggttac gatgaccgtc agtcagcatt
    21121 attcactaca acaaattgcc gcctgggcgc agattgacga atctcgctgg aaggagaaac
    21181 tcgcgaaatc acaagtgcgg gttgcagtta ttaatgcaaa actggtcggc tttattacct
    21241 gcgttgaaca ctatatcgat atgttatttg ttgaccctga atacacccgc cgtggggttg
    21301 ccagcgcttt gttaaaacct tttattaagt ctgaatccga acttacggtg gacgcaagca
    21361 taaccgcaaa accctttttt gaacgttatg gttttcagac agttaagcag cagcgcgttg
    21421 aatgccgggg agcgtggttt attaattttt atatgcgtta taaaccgcaa cattaaatcc
    21481 agcttgcaat gaaaataacg cccgcctagt atgtgcgcgt tttcttattc cacaaactgc
    21541 aaggaggtaa atcatgacaa atcctttatc aatgactctt tgcagacctt tccaggatta
    21601 attctttttt tcttgccctg gattcgtctg ccatttcctg attttttaat tgttactctc
    21661 aataattcaa gtcgcagcac atgcaacttg aagtatgacg agtctattta cggaatggta
    21721 tatgggcaat tatattcgtc ccttatccga tgcggtattt accatcgcat ctgatgacct
    21781 gtggatcgag agtttagcga tccaacaatt acacaccacg gcaaatttac ccaacatgca
    21841 gcgcgtagtg gggatgccag atttacaccc cggacgcggc tacccgattg gcgcagcgtt
    21901 cttctccgca ggccgttttt acccagcact ggtcggcaat gatatcggct gtggcatggc
    21961 gctatggcaa acagatattc tcgctcgcaa atacaacgcc gataagtttg aaaagcgatt
    22021 atcagctttg gatgacgttg ctgaagaaag ctggctggag gaaaacctgc cgtcagcatt
    22081 tgcacagcat ccgtggcgca gctcgcttgg ctctatcggt ggtggcaatc atttcgctga
    22141 actgcaacaa gttgatcaaa tcatcaacgc tgaactgttt gcactggcag gtctggatgc
    22201 gcagcatctg caactgctgg ttcatagcgg ctcgaggggg ctagggcagt ctattttaca
    22261 gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg acgcgctggc
    22321 gtttgcgcgt tttaatcgcc acctgatcgc tttgcgcata atgcaacagg ttaaggccac
    22381 cggaagtccg gttctggatg tggcgcataa ctttgttagc gcgtgtcgaa tcggtgatca
    22441 acaaggggtc ttacatcgta aaggtgccac gcctgatgac tgtggtctgg tggttattcc
    22501 cggttcacgc ggtgattact ccaggctggt tcagcccgtc aggagtgagg aaacattgca
    22561 ttcgctggcg catggggctg ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc
    22621 agcgaaatac acagcgacgc aactctcgcg tactgaactt ggcagtcggg taatttgtcg
    22681 cgataaacaa ctcatcttcg aagaagcgcc acaagcctat aaatcggctg aaagtgtggt
    22741 gcaatgtcag ggcaggccgg attaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg
    22801 ctcaaaaaca gtggagggaa aaaaggatga tcttgctata actctcctca gcgcaggggc
    22861 cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc
    22921 gacaagacgt cgcggtaacg gtgctggaaa cagaaacagg ccgctactct gacacgctgc
    22981 gttcggcgct gatttctctg gatggcgaca acgcatgggc gttaagcgaa agctggtgtg
    23041 gcactattca gtggatttgc ccgagcccgt atcgcccaca tcacgggcgt aaaaactggt
    23101 ttctgggcat tgggcgtttt accgctgatg agcaggagca atccgatgcg atccggtatg
    23161 agacgctgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac
    23221 gcgccacgca tttggcatcc ggtattagcg tgaaggttca gtcagagcgc agccagcatg
    23281 ctaacaaacg gctggcgcga ttgctgattg cctggaagct ggatcagcag caataggaaa
    23341 atagcgcggt gctgaaatca cagcggcgaa tgttccatca ccagattgaa cgtggcaacc
    23401 cgcgacgaac gtttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg
    23461 aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca
    23521 gcagttcagt cagcagtgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg
    23581 gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt
    23641 ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggc gtcttgttga
    23701 acaggcgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc
    23761 agccaggata tgcgcctttc gcttcggttt tcgcgccagc cagtttaccc agcgaatcca
    23821 gcatgctcac cacgtagtct ttaccgctgt cgattagtga acggatcagg cagtgaattt
    23881 ctacgttatt gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt
    23941 ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg
    24001 tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg
    24061 ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca
    24121 gagatttcag tgcgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg
    24181 ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgcaggtcc agttcttctg
    24241 catgacccgc caggaagcgc accagcagtt tgttggcatt acccaggcca acgtggattt
    24301 caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag
    24361 ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac
    24421 ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc
    24481 agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca
    24541 cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagtgca gaggccatac
    24601 caatgccgtt atccgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat
    24661 aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca
    24721 tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt
    24781 gacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc
    24841 aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga
    24901 ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa
    24961 gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt
    25021 gtcaacacaa gacaggcttg cgagatatgt attgagaata ccactttatc ccgcgtcagg
    25081 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
    25141 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
    25201 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
    25261 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
    25321 aagccgtggc ggtctggtac cgggggcgtt actggcgcgt gaactgggta ttcgtcatgt
    25381 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
    25441 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
    25501 tactgctgtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
    25561 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
    25621 cgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
    25681 tcaacgcctg gcattgccgg gcgttgttct ttttaacttc aggcaggtta caatagtttc
    25741 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
    25801 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
    25861 aaccgcctgt gcagtcggct cttgatggta aaaccatccc tcactggtat cgcatgatta
    25921 accgtctgat gtggatctgg cgcggtattg acccacgtga aatcctcgac gtccaggcac
    25981 gtattgtgat gagcgatgct gaacgtaccg acgatgattt atacgatacc gtgattggct
    26041 accgtggtgg caactggatt tatgagtggg ccacccaggc gatggtgtgg caacaaaaag
    26101 cctgtacgga agaagatccg caactcagtg gtcgacactg gctgcatgcg gctacgttgt
    26161 acaacattgc cgcctatcct catctgaaag gagatgacct ggccgagcaa gcgcaggctt
    26221 tatcaaaccg cgcctatgaa gaggccgccc agcgtctgcc gggcacgatg cggcagatgg
    26281 agtttaccgt acccggcggt gcgcccatca ccggcttttt gcatatgccg aaaggcgatg
    26341 gcccgttccc gacagtatta atgtgtggtg gtctggatgc gatgcagacg gactattaca
    26401 gcctgtatga acgttatttt gcgccgcgcg gcattgcgat gctgactatt gatatgccgt
    26461 cggtgggctt ttcttcaaaa tggaagctca cccaggactc cagcctgttg catcagcacg
    26521 tcttaaaggc gctgcctaac gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt
    26581 tccgtttcgg cgctaacgtt gccgtgcgtc tggcatacct tgaatcgccg cgtctgaaag
    26641 cggttgcctg tcttggtccg gtagttcata ccctgttgag tgattttaag tgccagcaac
    26701 aggtgccgga aatgtatctt gacgttctgg cgagtcgttt ggggatgcat gatgcttccg
    26761 acgaagcgtt acgcgtggag ctgaatcgct attcattaaa agtgcaagga ttgctggggc
    26821 gtcgctgccc aacgccaatg ttatcaggct actggaagaa cgatccgttc agcccggaag
    26881 aggactcacg cttaatcacc tcatcatctg ctgacggtaa attattagag atcccattta
    26941 acccggtgta tcggaatttt gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac
    27001 gcttgtgtta aaaatttgct aaattttgcc aatttggtaa aacagttgaa tcacaacagg
    27061 agatagcaat gacgttaccg agtggacacc cgaagagcag attgatcaaa aaatttaccg
    27121 cactaggccc gtatattcgt gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc
    27181 tggctgtatg cgtcaacgtg aaaccggcac cggaagtgcg tgaattctgg ggctggtgga
    27241 tggagctgga agcgcaggaa tcccgtttta cctacagcta ccagtttggt ctgttcgata
    27301 aagcaggcga ctggaagagt gttccggtaa aagatactga agtggttgaa cgactggagc
    27361 acaccctgcg tgagtttcac gagaagctgc gtgaactgct gacgacgctg aatctgaagc
    27421 tggaaccggc gaatgatttt cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg
    27481 ccggatgcat cacatccggc aatattcatt aaaactgata cgtcatccca accgcgacag
    27541 tatcatcatt attaatattc agcttattat cgctatccag ttggttgatt ttataatcaa
    27601 caaacgctga catatttttg ttgaaataat acgtagcacc gacgtcgata taattgacca
    27661 gatcttcatc accgatgccc tcaatatctt tccctttcga taagacataa cccagcgatg
    27721 gacgcagacc aaagtcaaac tggtattgag cgaccgcttc aaagttctgt gtcttattgg
    27781 caaagccgcc agttattggc gtcattttgc gtgtttcaga atagaaagtt gccagataaa
    27841 tattattggc atcgtatttc agacctgttg cccatgcttc tgcacgcttg cctgtgccac
    27901 ggctttgcag gttctgctcg ttggtgcgat ctgagttggt ataggcccca ctaatggcga
    27961 aatcgctgcc gccaaagtca tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt
    28021 taacgtcgcg gttttcgttt ttcccttgat attgcagggt taagttcagg ccatcgataa
    28081 cgccgaagaa gtcggtgttc cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt
    28141 tgtcggtctg cgccgaggaa tcgccaccaa attccgggaa catatcggtc caggcttcca
    28201 cgtcatacaa cgcgcccagg ttacgaccat aatcgaaaga acccaaatct ttatatttca
    28261 acccggcaaa agcgagacgc gttttttgct gtgcagtatc actctccgct ttatttccgg
    28321 caaactccgc ttcccaccga ccataaccag tcagttgatc gttaat
//
LOCUS       DAJTUP010000046        28010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTUP010000046 DAJTUP010000000
VERSION     DAJTUP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.046"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(128..1144)
                     /gene="phnD"
                     /locus_tag="OO468_002044"
     CDS             complement(128..1144)
                     /gene="phnD"
                     /locus_tag="OO468_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6259160.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(1169..1957)
                     /gene="phnC"
                     /locus_tag="OO468_002045"
     CDS             complement(1169..1957)
                     /gene="phnC"
                     /locus_tag="OO468_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR6259161.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            complement(2090..2533)
                     /gene="yjdN"
                     /locus_tag="OO468_002046"
     CDS             complement(2090..2533)
                     /gene="yjdN"
                     /locus_tag="OO468_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR6259162.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(2691..3026)
                     /locus_tag="OO468_002047"
     CDS             complement(2691..3026)
                     /locus_tag="OO468_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR6259163.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            3428..5656
                     /gene="crfC"
                     /locus_tag="OO468_002048"
     CDS             3428..5656
                     /gene="crfC"
                     /locus_tag="OO468_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR6259164.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            5653..6531
                     /locus_tag="OO468_002049"
     CDS             5653..6531
                     /locus_tag="OO468_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR6259165.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            6795..8297
                     /gene="proP"
                     /locus_tag="OO468_002050"
     CDS             6795..8297
                     /gene="proP"
                     /locus_tag="OO468_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR6259166.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            8409..8498
                     /gene="pmrR"
                     /locus_tag="OO468_002051"
     CDS             8409..8498
                     /gene="pmrR"
                     /locus_tag="OO468_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR6259167.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8474..9574)
                     /gene="pmrB"
                     /locus_tag="OO468_002052"
     CDS             complement(8474..9574)
                     /gene="pmrB"
                     /locus_tag="OO468_002052"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR6259168.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(9575..10243)
                     /gene="basR"
                     /locus_tag="OO468_002053"
     CDS             complement(9575..10243)
                     /gene="basR"
                     /locus_tag="OO468_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR6259169.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(10240..11883)
                     /gene="eptA"
                     /locus_tag="OO468_002054"
     CDS             complement(10240..11883)
                     /gene="eptA"
                     /locus_tag="OO468_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR6259170.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(11987..13324)
                     /gene="adiC"
                     /locus_tag="OO468_002055"
     CDS             complement(11987..13324)
                     /gene="adiC"
                     /locus_tag="OO468_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR6259171.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(13461..14222)
                     /gene="adiY"
                     /locus_tag="OO468_002056"
     CDS             complement(13461..14222)
                     /gene="adiY"
                     /locus_tag="OO468_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR6259172.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(14547..16814)
                     /gene="adiA"
                     /locus_tag="OO468_002057"
     CDS             complement(14547..16814)
                     /gene="adiA"
                     /locus_tag="OO468_002057"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR6259173.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(17013..17921)
                     /gene="melR"
                     /locus_tag="OO468_002058"
     CDS             complement(17013..17921)
                     /gene="melR"
                     /locus_tag="OO468_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR6259174.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            18204..19559
                     /gene="melA"
                     /locus_tag="OO468_002059"
     CDS             18204..19559
                     /gene="melA"
                     /locus_tag="OO468_002059"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR6259175.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELPRDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            19662..21083
                     /gene="melB"
                     /locus_tag="OO468_002060"
     CDS             19662..21083
                     /gene="melB"
                     /locus_tag="OO468_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR6259176.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(21222..21851)
                     /locus_tag="OO468_002061"
     CDS             complement(21222..21851)
                     /locus_tag="OO468_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR6259177.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(21974..23620)
                     /gene="fumB"
                     /locus_tag="OO468_002062"
     CDS             complement(21974..23620)
                     /gene="fumB"
                     /locus_tag="OO468_002062"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR6259178.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(23698..25038)
                     /gene="dcuB"
                     /locus_tag="OO468_002063"
     CDS             complement(23698..25038)
                     /gene="dcuB"
                     /locus_tag="OO468_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR6259179.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(25609..26328)
                     /gene="dcuR"
                     /locus_tag="OO468_002064"
     CDS             complement(25609..26328)
                     /gene="dcuR"
                     /locus_tag="OO468_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR6259180.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(26325..27956)
                     /gene="dcuS"
                     /locus_tag="OO468_002065"
     CDS             complement(26325..27956)
                     /gene="dcuS"
                     /locus_tag="OO468_002065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR6259181.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
BASE COUNT         6647 a         6849 c         7305 g         7209 t
ORIGIN      
        1 gggcgatggt gatggtttgc atgtttggct ccggtctgta ggccggataa ggtgcttgca
       61 ccgcatccgg catcaacgcc tacacattgt cggatgcgac gccggggcgt cttatccgac
      121 ctacgattta ctgcaccgct ttactcaccg aactcatcgc gcttaacgcg ttgttcaggc
      181 ggtccaggtc atccagctgc gcctgaatct cggtggtttt cgccagcttg tcctgctcat
      241 tcagcccttt attgcctttc acgctttgta tctctttaaa cagtgcgagc tggcgaatcg
      301 gcaccagttg caggtcgctg gaagcgcgga acggcgccca gcccaggcgt tccagcaccg
      361 ctttctcttc cggcgttttt ccgtagttca taaagaagtc gtagatcttg tctttggtag
      421 tttcggacag attcttgcgc cagacgatcg gatcgcctag gatcagcggc gatttccaga
      481 tcaccttcag ctctttaagc ttctccggcg cggaggtttt cagcttgtcg aggttttcgg
      541 tgttgttggt ggcaacatcc acttgcttgt tggcgacggc cagcgcgttg gtttcatgcc
      601 cggcgttgac ggtgcgcttg aagtcgctgg cggagatatt gtttttagcg aagacgtagt
      661 aaccggggac gaggaagcca gaggtggagt taggatcgcc attgccgaag gtgagatctt
      721 tccgcttcgc cagcagatcg ttcaggttgt tgatcgggct gtctttgttg acgatcaaca
      781 cgctccagta acccggcgat ccatccgccg cgaccgtctg ggcgaagacc tggccattgg
      841 cgcgatccac cgcttccatc gccgacaggt tgccgtacca ggcaatatcc actttattga
      901 agcgcatccc ctggataatg cccgcgtagt ccggggcaaa gaaggcgttg accttcacgc
      961 ccagtttctt ctccatatcc tgtaaaaacg gcgtccattg cggtttcagg ttttgctgtg
     1021 attccgttga aataatgccg aaattcagcg ccttttcctg ctcttcggcg tgtgccgggg
     1081 ttaacagggt gctgaggctg aacatgctgg tgaaggccag cgaggcaatt atcttagcgt
     1141 tcattcgttt tcctcaatga tggggatatc aggcagcttt cgcgttctct tcgatgcggt
     1201 taatgctgcg gtagagatgg tcaaaacgtt cgttatcaaa cagttggctg ctgccgtcgt
     1261 agaagacgtg cccctgacgc agggcgacga tgcgttcgca gtagcgcagg gcgtaatcca
     1321 cctgatgcag cgtgacgacc acggtgatgc cgtcgttctg gttgatgtcg cgcagggtgt
     1381 ccatcacgat gcgcgctgat tccgggtcca gcgaggcgat gggttcatcg gccagaatca
     1441 ctttcgcctg ctgcatcagc gcacgggcaa tcgccacacg ttgctgctgg ccgccggaga
     1501 gggtggaaac gcgctgatgg gcaaaatgca ccatgccaac gcgggtcagc gcctgtaacg
     1561 cgcgctgttt ctgctcgccg gtgaaccagc taaaacaggt gcgccagaac ggcgtgctgc
     1621 cgagcgcgcc aatcagcacg ttctccagta cgctcaggcg gttcaccagg ttgaattgtt
     1681 gaaagatgta gccggtgttg gcgcggcttt tgcggatatc gcgggccagg cggccttcgc
     1741 gctggacagt gcggcccagt agctcgatat ggctaccggc ggatttatcg ccggtaatca
     1801 aaccgcttaa atgacgtaaa agggtggatt tgccggaacc cgacggccca agcagagcca
     1861 ccatttcact gtgatgaatg ttcagatcaa ccgcatgcag cgcttgatgc tgattgaagg
     1921 ttttggcgag cttctcgaca cggataatcg tttgcatgat gcagcctccc taaaaaagtg
     1981 gctccatcgt ggcggattat tgtgacgatt tggttaatta aaagtgacta acagatgaag
     2041 agttaacggg aattcgatga cagtgcggga gggcgggcct cccggcgggt tattgcgttg
     2101 gttgttgttt gacgacatta atcatccacg gtacgccaaa tttatcggtg actttgccaa
     2161 agccatgcgc ccagaaagtt tcctgccagg ccatttcgat ttttccgtta gcggcaaggt
     2221 tatcaaacca gcgttttcct tcttcgacct gttgcgaatc gagcaccagc gtaaagccgg
     2281 agtagctggc ttttcctgac ggtatagcat cgctcatcat gatgtcgctt ccggcaatgc
     2341 gcacgttggc atgagcgatg gcggtatcgg gaaattgcat tccggaaggg cagttctcgg
     2401 cgctgtcctg cgctgatttc ggcatttcgc cgaagctgat tttatagagc agttccgcgc
     2461 ccaacgtacg ttgataatag gcaatcgcgt cggaacagtt accggcaaaa gagaggtagg
     2521 gacttaacgg catgatggtg acctcagtta agagaagcca gttaagtgta gttcgaaatt
     2581 tatacagatg agaggctgcc tgataagacg cgcaagcgtc gcatcaggca gtcggcatcg
     2641 ttgccggatg cggcgcgagc gccttatccg gcttaccgat cacaatacaa tcagttcttt
     2701 ttcacaaact cagatttcag tttcatcgga ccaaaaccgt cgattttgca atcgatgtta
     2761 tggtcgcctt caaccaggcg gatgtttttc actttggtgc caattttcag catcgaagag
     2821 ctacctttca ccttcagatc tttaatgatg gtaacgctgt cgccgtcagc cagcagattg
     2881 ccgttagcat ctttaacgat cagctcgtcg ctttcctgtg caggttctgc gtcgttccat
     2941 tcgtaggcac attccgggca gatgtacatg ccgttatctt cgtaagtgta ttcggagttg
     3001 cattttgggc agtgtggtaa tgacatgtgg atttcctcaa aagtcagcag ggcaaaagcg
     3061 ccaaaaaacg gcagattgcc gaaaaaggcc gcaattatac acaaaatccc tgagtttgtc
     3121 gggggtactg ctgattttag acgacgatcg ttttcatttg aaaggaaacg caaaaagaaa
     3181 ataatctttt tgcctctatt tattatttaa gataatgtta atgctctact tgttcttttt
     3241 ttattcacat gatattttta tctttctgac gttttaaacg catcagaaga cgttatttat
     3301 tctgataaaa aaacaacaca ctaaatataa aattatttct tgcagtaatt ataaaagccg
     3361 aaaacagaac tcaaaaacaa tctggctaaa taaaagtaac aaaatttgct ttaaggaaga
     3421 attttctatg tacacacaga ccctgtatga gttaagtcag gaggctgaac gcctgttaca
     3481 gctttctcgc caacagttgc agttactgga aaaaatgcct ctctctgtac ccggagacga
     3541 cgcgccacaa ctggctttac cctggagtca gcctaatatc gccgaacgtc acgcgatgct
     3601 gaataatgag ttgtgtaaaa tttcccgact ggaaatggtg ctggcaattg tcggtaccat
     3661 gaaagcaggg aaatcaacca ccattaatgc cattgttggt acggaggttc tgcctaatcg
     3721 taatcgccca atgactgcgc tgccgacgct tattcgccat acgcccgggc aaaaggaacc
     3781 ggtactgcat ttttcacatg tcgcgccaat cgattgttta attcaacaat tacaacagcg
     3841 cctgcgtgat tgcgatatta agcatctgac cgatgtgctg gaaatagata aagatatgcg
     3901 tgcgcttatg cagcggatcg aaaatggcgt cgctttcgaa aaatattatc tgggtgccca
     3961 gcctattttt cattgtctga aaagtttgaa tgatttagtg cgactggcga aggcgctgga
     4021 cgtcgatttt cctttttctg cttacgccgc cattgagcat attcccgtga ttgaagtgga
     4081 gtttgtccat ctggcggggc tggaaagtta tcccggtcag ttgacgttac tggatacccc
     4141 cgggccaaat gaagccgggc aaccgcatct gcaaaaaatg cttaaccagc agctggcacg
     4201 cgcctcggcg gtactggcgg tgctggatta tacgcaactg aaatcgatct ccgatgaaga
     4261 ggtccgtgag gcgattttgg cggtggggca atcggtgccg ctgtatgtgc tggtcaataa
     4321 gttcgatcaa caggatcgta acagtgacga cgccgaccag gtgcgggcac tgatttccgg
     4381 gacgctgatg aaaggctgta ttacgccaca gcagatattc ccggtgtcgt cgatgtgggg
     4441 ctacctggcg aatcgggcgc gctatgagtt agccaacaac ggtaagttac cagcgccaga
     4501 gcaacaacgc tgggtggaag attttgccca tgccgcgctc ggcaggcgct ggcgtcatgc
     4561 cgatctggcg gacctcgaac atattcgtca tgctgccgat cagttgtggg aagattcgct
     4621 gttcgcccag ccaattcagg cgttgcttca tgccgcttac gctaacgcct cattgtatgc
     4681 tctgcgatct gctgcgcata aactgttgaa ttacgcgcag caggcgcggg aatacctgga
     4741 ttttcgtgcg cacgggttaa acgtcgcttg tgaacaattg cggcaaaata tccatcaggt
     4801 cgaagaaagt ttgcagctat tgcaactcaa tcaggcgcag gtgagcggcg agattaaaca
     4861 tgaaatcgag ctggccctga cctccgccaa ccactttctg cgtcaacagc aagatgcggt
     4921 gaatgcgcag ttagccgcct tgtttcagga tgattcggag ccgttaagcg agattcgtac
     4981 ccgctgtgag acactgttac agacggcgca gaacaccatc agtcgcgact ttacgctgcg
     5041 ttttgccgag atggaatcca ccctttgccg ggtgttaacc gatgttattc gccccattga
     5101 gcaacaagtc aaaatggaat tgagcgagtc agggtttcgt cctgggtttc attttcctgt
     5161 ttttcacggc gtagttcccc acttcaacac tcgccagctg ttcagtgaag tcatttcgcg
     5221 ccaggaagca acggacgagc agagcacgcg tttaggcgtt gtgcgtgaga ctttttcgcg
     5281 ctggttgaat cagcccgact ggggacgggg aaatgagaaa tccccgacag aaacggttga
     5341 ttacagtgtg ttgcaacgag cattaagcgc agaagtcgat ctctattgcc aacaaatggc
     5401 taaagttctg gcagagcagg tcgatgaatc tgttacggca ggcatgaata cttttttcgc
     5461 tgagttcgct tcatgtttga cagaattaca gacgcgttta cgcgaaagtc tggctctgcg
     5521 tcaacaaaat gaatcggtgg tcaggctgat gcagcagcaa ttgcagcaga ctgtgatgac
     5581 tcacagctgg atttacaccg acgctcagct gttacgcgat gatattcaaa cacttttcac
     5641 ggcagaacga tattgaccaa gacgttactg gacggccccg gtcgcgtgct ggagtcggtt
     5701 catccgcgct ttttagtgga tctggcgcag ggcgatgatg cccgccatcc tcaagcccat
     5761 cagcagcagt ttcgtgaacg actgatgcag gaacttcttt cgcgtgtgca gcttcagaca
     5821 tggacgaacg gcggcatgtt aaatgcgccg cttagcctgc gactgacaat ggtggaaaaa
     5881 ctggcgtcga tgctggatcc cggtcatctg gcgctgacgc agatcgcgca gcatctggcg
     5941 ctgctgcaaa aaatggatca ccgccagcac tctgctttcc cggagctccc ccagcaaatt
     6001 gccgccttgt atgagtggtt ttcagcccgt tgtcgctgga aggaaaaggc gttaacgcaa
     6061 cgaggcctac tggtgcaggc aggtgatcag agcgagcaaa tttttacccg ctggcgtgct
     6121 ggggcgtata acgcctggtc gttgcctggg cgctgtttta tcgttctgga ggagttgcgc
     6181 tggggtgcat ttggcgatgc ctgccgtctg ggaagcccgc aagcggtggt gttgttgctg
     6241 ggtgatttgc gcgagaaagc gacacaacat ctggcagaga gtatcaatgc ggcaccgact
     6301 acgcgtcact attaccatca gtggtttgcc tcttcgaccg ttccgacggg cggggatcat
     6361 gctgattttt taagttggct gggaaagtgg accacggcag ataaacaacc cgtttgctgg
     6421 tcagtgaccc aacgctggca aactgtcgcg ctggggatgc cacgactctg ttcagcgcag
     6481 cgtttggtgg gggcaatggt cgaggaaatc ttctctgtaa atttggcgta aataatcagt
     6541 tacatcaatg agtcctaaac gaaatccatg tgtgaagttg atcacaaatt taaacactgg
     6601 tagggtaaaa aggtcattaa ctgcccaatt caggcgtcaa ctggtttgat tgtacattcc
     6661 ttaaccggag ggtgtaagca aacccgctac gcttgttaca gagattgcat cctgcaattc
     6721 ccgctcccct tttgcggccg tcgcgctgat ttttctggcg tttgcggaaa tgggccaact
     6781 ctgcgaggaa agctatgctg aaaaggaaaa aagtaaaacc gattaccctt cgtgatgtca
     6841 ccattattga tgacggtaaa ctgcgtaaag ccattaccgc agcatcactg ggtaatgcaa
     6901 tggaatggtt tgattttggt gtttatggtt ttgttgctta cgcattaggt aaagtttttt
     6961 tcccgggggc tgaccccagc gtgcagatgg ttgctgcact tgccactttc tccgttccct
     7021 ttctgattcg accgcttggc ggactcttct ttggtatgtt gggcgataaa tatggtcgcc
     7081 agaagatcct cgctatcact attgtgatta tgtcgatcag tacgttctgt attggcttaa
     7141 taccgtccta cgacacgatt ggtatttggg caccgattct gctgttgatc tgtaagatgg
     7201 cacaaggttt ctcggtcggt ggtgaatata ccggggcgtc gatatttgtt gcggaatact
     7261 cccctgaccg taaacgtggc tttatgggca gctggctgga cttcggttct attgccgggt
     7321 ttgtgctggg tgcgggcgtg gtggtgttaa tttcgaccat tgtcggcgaa gcgaacttcc
     7381 tcgactgggg ctggcgtatt ccgttcttta ttgctctgcc gttagggatt atcgggcttt
     7441 acctgcgcca tgcgctggaa gaaactccgg cgttccagca gcatgttgat aaactggaac
     7501 agggcgaccg cgaaggtttg caggatggcc cgaaagtctc gtttaaagag attgccacta
     7561 aatactggcg cagcctgttg acatgtattg gtctggtaat tgccaccaac gtgacttact
     7621 acatgttgct gacctatatg ccgagttatt tgtcgcataa cctgcattac tccgaagacc
     7681 acggggtgct gattattatc gccattatga tcggtatgct gtttgtccag ccggtgatgg
     7741 gcttgctgag tgaccgtttt ggccgtcgtc cgtttgtgct acttggtagt gttgcactgt
     7801 ttgtgttggc gatcccggcg tttattctga ttaacagtaa cgtcatcggc ctgatttttg
     7861 ccgggttact gatgctggcg gtgatcctta actgctttat gggcgttatg gcttctacct
     7921 tgccagcgat gttcccgacg catatccgtt acagcgcgct ggcggcggca tttaatattt
     7981 cggtgctggt tgccggtctg acgccaacac tggcggcctg gctggtcgaa agctcgcaga
     8041 atctgatgat gcctgcctat tacctgatgg tagtggcggt gattggttta atcaccggcg
     8101 taaccatgaa agagacggca aatcgtccgt tgaaaggtgc gacaccggcg gcgtcagata
     8161 tacaggaagc gaaggaaatt ctcgtcgagc attacgataa tatcgagcag aaaatcgata
     8221 atattgacca cgagattgcc gatttgcagg cgaaacgtac ccgcctggtg cagcaacatc
     8281 cgcgaattga tgaataagct gaaacggatg gcctgatgtg acgctgtctt atcaggccaa
     8341 ttgaactctt aaggttcact taatctctga cgcgcatact ctcctccagg ttaacggagg
     8401 agagtgcaat gaaaaaccgt gtttatgaaa gtttaactac cgtgttcagc gtgctggtgg
     8461 tcagcagctt tctttatatc tggtttgcca cgttctgatc tttcttcagc cgtacccagg
     8521 cccgcgtgcc ggaagtctct tgccggtttt gcaggaaaaa ctgcccgtga tgcaactgtg
     8581 taatgcggct gacaatactt aaccccagac caatcccgcc ataacggctg tccatacgta
     8641 caaacgcttt actcaactcc ccgcatttac tctcatcaat acctggtcct tcatcttcaa
     8701 ctgccatgac cgctccgcca tcttcttgca gcttaatcat aatgttgctg ccttgcgggc
     8761 tgtaacgatg ggcgttttct accaggtttc gcaataacat ccgcagcagg gttgcatcac
     8821 cctgaacggt gatgtcggcg gcgctctctg gcaatagcag ggtttgctgt cgctggtcga
     8881 gcatggtact gagttcgtca tacgagggga gaatgacatc ttccagcagt tttacatgtt
     8941 gataattacc ggaagaaaat gactgtccgg cacgcgccag ttgcagcagc tgggagacgc
     9001 tctccatcat ctgatcaagc cgtgccacta acggtgctac atcaatgtga tgcgttttcg
     9061 ccagcagttc cagatgcaaa cgcacccccg ccagtggcgt tcgcagttcg tgcgcgacgt
     9121 cagcggtaaa caacctttcg ttatccagcg tgctggtcag gcgactgacc agatcgttca
     9181 acgccgaaac caccgcttcg atttcgaggg tggcgctgtg aatggcaatg ggcgttaagt
     9241 tgtcggcggt gcgcgcttcc agctcttttt gcagctccgc cagcgggcgg gtgatgcggc
     9301 gtaccgcctg atagcagata aatagcgtca ggctgaccat aaagacgccg gggacaatca
     9361 ggctggcgac cgcctcgcgg atctcacgca tgatgtggcg atcgttgttg cgattgtcgc
     9421 gtagcgcctg ctcaaacagc tgaatctgct cggtactttc atgccatagc cagaagacgc
     9481 tgatcagctc aaacaccaac aaaatggccc cgatggtcaa tatcagccgt tggcgcagcg
     9541 atataggtcg gcgcagaaaa cgcatcagat tcaattagtt ttcctcattc gcgaccagca
     9601 tatagccaaa gccgcgcacg gtgcggatac gggctttgcc cactttgtcg cgcagattgt
     9661 ggatatgcac ttccagggtg ttggtcgagg gttcattgtc ccagttatag atgtcgttgt
     9721 agagaatttc ccgatgtacc ggactgcctg ctttgagcat taaccgtgac agcagagcat
     9781 attctttggg cgtcagaatc aactcttcac cgcccatcca tacctgacgg cgacccatat
     9841 tcagcgtcag attgccaaca atcagctcac tttcgccctg attattatgg cgtcgtagca
     9901 gggcgcggat acgggcgtgt aactcttcca gcgcaaaagg cttcaccaga tagtcatcgg
     9961 caccgacatc cagcccggcg attttgtcgg tcagcgtatc gcgagcggtg aggatcagta
    10021 ccggcagggt gtattttttc tgccggatac gggcgagaaa atgcagtcca tcttcgtcgg
    10081 gtaaccctaa atccagtacc accaggctgt aatgaccgtc ctcaaggctt tgttccgcca
    10141 tccgcgcggt tgtcacgcca tcgcacgcgt agccttcggt ttgcgccgcc agaatcagtc
    10201 cctgcaataa cagcgtatcg tcttcaacaa tcagaatttt catttactca ctctcctgca
    10261 agtttgcaga atatcatccg cggcctggta atacttcgtc tcaacgccag ttaatcccaa
    10321 tagcgtggag aataaattgt cttgtgaata gtgttgcgtt tgcgcctgtt tttgcaggca
    10381 gttctggtca acctgatacc gcttttgata atcctccgac agccacagca gcattggcac
    10441 ctgtttttgg ctatccgggg cgatggcata aggcagaccg tgcagataga tgccattttc
    10501 acctaacgat tcaccgtggt cagaaagata aaccaggctg gtggtaaatt tatcctgatg
    10561 ttctttaagc agattaatcg ctttatcaac aatatagtcg acgtaaacca gcgtgttgtc
    10621 gtaagtgttc accagttgct ctttggtaca ggtctggatc tcattggtgt cgcaggttgg
    10681 ggtaaatttc ctgaactgcg gcggatagcg gttgtaatag gtcggaccgt ggctgccgat
    10741 ggtgtgtaag acaatcacgc catcaccctg caggttattg atgtactctt ccagcccgtg
    10801 gaacagcact tcgtcatagc attcgccgtt gatgcactga tcaggcagat tcagcgcggt
    10861 gacgttctgg tgaggcacgc ggtcgcaggc acctttacag ccgccatcgt tgtcattcca
    10921 cagcacgttg atgcccgctc gctgaatgat atccagcacg ccttcctggt gctgtgccag
    10981 ctcttctttg tagtgctcac gcggcatatc cgagaacatg cacggtactg aaactgccgt
    11041 tgccgtgccg caagatgcgg tattagggaa atagaccacg ttatctttcg ccagccgcgg
    11101 gttagtttca cgcgggtagc cgttgaggga gaagttctcc gcccgcgagg tttcgccgac
    11161 aatcaggatg gtcaaatttt tacgtttttc gttctgcatt aacgggttgc ggtgcgcgtc
    11221 ttcaccaatt cgcaccagcg gcagatttgc cagtcgctga tgggagtacc atgaccagct
    11281 ggcaacaatg ctgttagagg ggcttaagga tttcaccagc tctttgttat tgcggaacaa
    11341 cgaggcgtag tctttataaa acagtgcggc gaccagcaaa atcagcagta cagaaaccag
    11401 aatattggct ccacggaaaa gaacactgcg cagacgcgag gtggcaggtt tgatttttag
    11461 ccagcaggca atcagctcag caagcacgcc gctgaatccc agcgttaata acatttgcgg
    11521 tgtcatcagc gcataacttt ctgctggagt ggtatcaata atattggcaa tcatcgagcg
    11581 gtcgatgacg atgccgtaag tcattatgaa atattgtgca gccgcgccaa tcagaataaa
    11641 caggcaggcc agtggtcgat taagccataa gaaagagctt agtgtcagga caatattaat
    11701 cacgctgaaa gcgacaaccg gcatcgacaa gaaaaccagt acgttatgca gcgaatccag
    11761 cggcagcgcc tgcaacacct gtttaaaaaa ggcaatattc aggcagatag agatataaaa
    11821 agcggccaac aatagccagg cgagtaaatt caaagagggt ctttttagta ggcgcttcaa
    11881 catcacggtg tttccatcga acaaagtgcg catatgctcg caaagcaaaa ttaagccaac
    11941 cttaagttct taaggttggc ttttatgttt gctggattaa ggggaattaa tctttgctta
    12001 ttggtgcatc taagggatac gggtttttat gtagccggtt gtaattcagc gcatacatgg
    12061 cggtgatgac catcagggtg acaaatgacc acataacctc tttcgccccg gaccccacca
    12121 cggcccaaat gcagtagagg aaggcaatgg tagtaacagc cagatatgcc gggcgtgctt
    12181 taccaaagtg accgtggccg agcagcagta acgccgcaca ggtgtaaaga tatggcacca
    12241 gtgtaaagat gaccgagacg gaagaaacca gaccgaactc tttggtcgcg tttggtgaaa
    12301 tgctgctgag ctggaagatg gtcatcaaaa taccgacgat aatcagcccc gccactggcg
    12361 tacccgcttt atttacacgg gcaaaaatcg gtgggaacag tccgtcatcg gcagcggctt
    12421 tcgccgtttg acccgccagc aacgtccagc cgcccagtga acctaagcaa cccgcagctg
    12481 cgcagaagga aacaatggcc ccagcggtgt cacccaacgc catccgtgcg gcatcaccga
    12541 atggcgaggc agaaacgcgc agtgcggcat tagggatcat ccccataatc gcggtggtgg
    12601 agagcacata gcaaacggcg gcaatcaata ccccaccaat ggtggcgata gggacattgc
    12661 gtttcgggtt tttcaccaca cctgcggcaa cggaggcact ttccacaccg atgaacgacc
    12721 acagcgtaac gttaagggta ctttgaatcg caccgaaggt gcccaggccg ctgacgttcc
    12781 atgccgccat ataggtttca ccacggaacc agaaccagcc aaatacggca atcccgacga
    12841 tggggatcag cgccagcaca gtggcaacgg cctgcacgcg ggtgatcatt ttcggaccga
    12901 caatgttcag caggacgaag atccacagca ccacgacgca ggtgatggtt aataccaatg
    12961 gatctttcag aatcgggaag aagtaactta aatatcctac gccaatgacc accatggcga
    13021 tattgccgat ccagcaggcc agccagtaga ggacgttggt ttgataaccg agaaacgggc
    13081 caaagcagcg gcgggcgtaa gcgtaagaac caccaggact tgggtcgagg aacgacattt
    13141 tggcgtatac catcgagagc cccagcgcac cgataatcgt caccaaccat ccataaatgg
    13201 caatcccgcc agtggaggcc aggtttgcag gtaacagaaa aacacctgac cccataatat
    13261 tccccgacac catcagggtg acggggatta agcccacttt gtgagcatca gcatccgaag
    13321 acataattaa actcctgcga aggcgagctt cgtgacaata aattacgtca tatcatacgc
    13381 ctgcattcga tagttgaatt attatcgacc gggttatcgc tgatgccgtt attaatatag
    13441 ctcttcgtta aggttaaaaa tcaggcgaca gtgcgttctc tgtgctgact aacataatgc
    13501 agcggcgtca taccgtagaa gtctttaaag acagaaataa agtacgacgt actgttgtag
    13561 ccgcaggact gtgatacctg agagatattt ttaccgtcca tcattaattc atttacggca
    13621 taacgcatgc ggcaggtggt gattatttgg ctataactgg tgttttcgct tttcaacttt
    13681 ttctttaaca gacttgggct aagacataaa caactggcta ccatactaag attccagtct
    13741 ttgtgaatat cgctttcaat aatttgataa acgctgtcac ttacacaatt acgtaacata
    13801 tacatcatta atgaaaggaa ttttttactg tcgagaaaac gggatagcac cgtgaataat
    13861 aatgcgcgtg ttcgttcctt ttctgactca gtttctgcgg gcatcatgct gtgttgcgcg
    13921 gcaacgcgaa agacgtctgg cgtcaggcat ggcagggtga gtatgggcgt agcgctacgt
    13981 tgccatactg gtatttgcga gagatcttta ttcagaaacc ggaggtaatc tttgatgatg
    14041 tcgtgactaa tatgagcaac caaagtgttg ttaagagagg agacgtcgat aatattattt
    14101 tcacaattta ataacgccat atggttagct ttaaggctaa taggttcttt cccattcacc
    14161 cttatccaga catctttttc agtcaataaa acaatacaag gttggtcgct gcaaatcctc
    14221 atacatgtac tcctgagtgc gaataaaaga ccagttctcg ctaaagcaaa gcgataccgg
    14281 gaaatggtta tataattgca tgatgaatat aaacatgcat aaaaataacg agttttacta
    14341 atattacaaa tttttatagt aatttctcat gtacatattt ttttacagga taaataacag
    14401 gcaaaaaaaa gcgtggggtg gggccgcgca aaaagtatga catttttgtt tgcagcaatt
    14461 tttatgaaaa cgttgccata ctcaaaaggc ctaaggccag gcggcgtaat gttatttaaa
    14521 caattacgcc ttcagcggaa tagtggttac gctttcacgc acataacgtg gtaaataccg
    14581 tcaataattt cagtcccttc agtttcgtgt tcaaatctag ggaaatggtg gtcccaggat
    14641 tgcagcgagc gtaaataact tacttgcgga ctgtttttat cgccgaagtt ttcaccagac
    14701 agcagcatcg ggattcctgg cggatacggg ataactgagt ttgccgcgat gcgtcctggc
    14761 agattttcaa tggataccag ttcgacattg ttgtcgacaa tcgcgttgta cgcttcacgc
    14821 ggggtgattt ccgccaccgg caggccggaa taggcttcgt tcaaccgtgc gccagggttg
    14881 ttttctttca gccaggcaaa catggtgtca cccagatcgt gaatccccat gttcgcgtaa
    14941 gtgtcaggat attgttcaac aagttccggc atcacctgcg ccagcggtgt gttggcgtca
    15001 tagtggtgtt tgaaggagca aagggtgtta accagagtcc cccatttccc acgggttacg
    15061 cccatagaga acaggaacat aatttggaag tcagtggtgc gggtaggcac aatgccgtgg
    15121 cgaccaagcc aggcagtgac cagcgccgcc ggaacaccgg tttcttccag ttcaccatct
    15181 tcacccattc ccggagcaag gatgctgact ttaattgggt cgagcatact ccagttatcc
    15241 ggaatatctt tgaagccgtg ccagctttcg cccggatgca ttacccagca gtcctgaacg
    15301 gtggtcagca gtttggttgg tgcgtcagca aagtcatagg ttttgccggt ttgtgggtcg
    15361 gtgacgactt ctttgttcca cggtttgaag aaccagctac cgtcagcggt gaactcttta
    15421 tatagccgcg ccatcgcctg acggaaatca accgcttcgt caatcacttc ctgtgtcagt
    15481 gacaggccgc tgttgccgtc catcatcgac accgccacgt cgttggatgc gcagatggca
    15541 tacagcgggg aggtggtggc atgcatcatg taggcctggt tgaagcggga gaagttaatc
    15601 gccccacgac cttcacgtac atgaatataa gaagcctgtg acagcgcatt cagcagtttg
    15661 tggatggagt gggtggcgaa aacggtagga ccgttgtgat cgccaggttc gccgcgcatg
    15721 gcatagtgat cggcatagat cgggttgaaa cgtgcatagc cgtaccaggc ttcgtcaaag
    15781 tgcagacgat cggaggtttt ttccagcaga tcctgcgctt ctttagcgtt ataacacacg
    15841 ccgtcatagg tgcagttggt taccacgcag taagacggtt tttgcccggc tttgtctttg
    15901 gtcagcgggc tttcactgat tttcttttgc aaggtttcag gttgcatttc ctgcggatag
    15961 attggcccga taatgccgta gcggttgcgg cttggcacca tatagaccgg tttcgcgcct
    16021 gtcagcatca aaccttgttc gatggattta tggcagttac ggtcaacgac cacgacatcg
    16081 ttatcggtca tgcaagcctg catgatggtg cggttagagc cggaagtacc gacgactacc
    16141 gaccaggagc gatcggcacc aaatacgcgt gcggcatatt tttcgctttc gccaaatgcg
    16201 ccagtatggt caagcaaaga accgagggaa gttcgttcga tgcccatgtc ggtacggaac
    16261 agattttcac catagtagtc atggtagaaa cgtccggcgg gtgttttggt aaaaccaacg
    16321 ccgccctggt ggcctggcgc tgcccaggaa tattcatgga tgtcactata tttcatcagc
    16381 gcgctgaaca gtggcggcaa cagctgctgg cggtagcggg tcatcgcggc aacggcgcgt
    16441 ccggcgataa agtcggcggt atcttccaga atccaggcga attcatcgac aagctccagc
    16501 agatcccggt ccattgcggc gagggctttt tcccgatcgc ccaacaggaa gaccggcacg
    16561 ttttgttggc gctcatgaag cttaccgatc aattgtctga cgttttgatg ttcgtccgga
    16621 tgctccattt gatagctgaa catcaggcag tcaatggctt cgtttgaaga gagaatggca
    16681 aaaccatcat caaaggaggt ggatttaatc acggtaacat tttgctggct taaagcatct
    16741 gccagacgct caacggcgtt accgacccag gtgtcttgat ggagaaactc gctttcaaca
    16801 attaatactt tcatcattgc ttacccggtt atgaaggaat cttcgttgcg ggcaagtatc
    16861 ttccggcctt ttcgggctgt aaagcgcgtg aaaaacaaac atttttgaaa tatttagttt
    16921 tataaacaat acgttatgtg attattttaa attattttca ctgcttatta tcgacggcta
    16981 aactattttt ttggctgata ctgatatcgt ctttagccgg gaaacgtctg gcggcgctgt
    17041 tggctaagtt tgcggtattg ttgcggcgac atgccgacat atttgccgaa cgtgctgtaa
    17101 aaacgactac ttgaacgaaa gcctgccgtc agggcaatat cgagaatact tttatcggta
    17161 tcgctcagta acgcgcgaac gtggttgatg cgcatcgcgg taatgtactg tttcatcgtc
    17221 aattgcatga cccgctggaa tatccccatt gcatagttgg cgttaagttt gacgtgctca
    17281 gccacatcgt tgatggtcag cgcctgatca tagttttcgg caataaagcc cagcatctgg
    17341 ctaacataaa attgcgcatg gcgcgagacg ctgtttttgt gtgtgcgcga ggttttattg
    17401 accagaatcg gttcccagct agagaggcta aatcgcttga gcatcaggcc aatttcatca
    17461 atggcgagct ggcgaatttg ctcattcgga ctgtttaatt cctgctgcca gcggcgtact
    17521 tcaaacgggc taagttgctg tgtggcaagt gatttgatca ccatgccgtg agtgacgtgg
    17581 ttaatcaggt ctttatccag cggccaggag agaaacagat gcatcggcag attaaaaatc
    17641 gccatgctct gacaggttcc ggtatctgtt agttggtgcg gtgtacaggc ccagaacagc
    17701 gtgatatgac cctgattgat attcactttt tcattgttga tcaggtattc cacatcgcca
    17761 tcgaaaggca cattcacttc gacctgacca tgccagtggc tggtgggcat gatatgcggt
    17821 gcgcgaaact caatctccat ccgctggtat tccgaataca gcgacagcgg gctgcgggtc
    17881 tgtttttcgt cgctgctgca cataaacgta tctgtattca tggatggctc tctttcctag
    17941 aatatcagaa ttatggcagg agtgagggag gatgactgcg agtgggagca cggttttcac
    18001 cctcttccca gaggggcgag gggactgtcc gagcatcatg aggccgaaaa ctctactttt
    18061 caggtaattt attcccataa actcagattt actgctgctt cacgcaggat ctgagtttat
    18121 gggaatgctc aacctggaag ccggaggttt tctgcagatt cgcctgccat gatgaagtta
    18181 ttcaagcaag ccaggagatc tgcatgatgt ctgcacccaa aattacattt atcggcgctg
    18241 gttcgacgat tttcgttaaa aatattcttg gtgatgtgtt ccatcgcgag gcgctgaaaa
    18301 cggcgcatat tgccctgatg gacattgacc ccacccgcct ggaagagtcg catattgtgg
    18361 tgcgtaagct gatggattca gcaggggcca gcggcaaaat cacctgccac acccaacaga
    18421 aagaagcctt agaggatgcc gattttgtcg tggtggcatt tcagattggc ggttatgaac
    18481 cttgcacggt gactgatttc gaggtctgta agcggcatgg tctggaacaa accattgccg
    18541 atacgttggg gccgggcggt attatgcgcg cgctacgtac cattccgcat ctgtggcaaa
    18601 tttgcgagaa catgacggaa gtctgccccg atgccaccat gctcaactat gttaacccaa
    18661 tggcgatgaa tacctgggcg atgtatgccc gctatccgta tatcaaacag gtcgggctgt
    18721 gccattcggt gcagggaacg gcggaagagc tgccgcgtga cctcaatatc gacccggcta
    18781 cgctgcgtta ccgttgcgca ggtatcaacc atatggcgtt ttacctggag ctggagcgca
    18841 aaaccgccga cggcagttac gtgaatctct acccggaact gctggcggct tatgaagcag
    18901 ggcaggcacc gaagccaaat attcatggta atactcgctg ccagaatatt gtgcgctacg
    18961 aaatgttcaa aaagctgggc tacttcgtca cggaatcatc agaacatttt gccgaatata
    19021 cgccgtggtt tattaagcta ggtcgtgagg atttgattga gcgttataaa gtaccgctgg
    19081 atgagtaccc gaaacgctgc gtcgagcagc tggcgaactg gcataaagag ctggaggagt
    19141 ataaaaacgc ctcccggatt gatattaaac cgtcacggga atatgccagc acaatcatga
    19201 acgctatctg gactggcgag ccgagtgtga tttacggcaa cgtccgtaac gatggtttga
    19261 ttgataacct gccacaagga tgttgcgtgg aagtagcctg tctggttgat gctaatggca
    19321 ttcagccgac caaagtcggt acgctacctt cgcatctggc cgccctgatg caaaccaaca
    19381 tcaacgtaca gacgctgctg accgaagcta ttcttacgga aaatcgcgac cgtgtttacc
    19441 acgccgcgat gatggacccg catactgccg ccgtgctggg cattgacgaa atatatgctc
    19501 ttgttgacga cctgattgcc gcccacggcg actggctgcc aagctggttg caccgttaaa
    19561 acgcgactaa acgctactgc gccgggggat ttattccggc gcacacctct gacgatacca
    19621 ataacagaag gcgggcgttg gtaacagcga cccgataccc tatgagcatt tcaatgacta
    19681 caaaactcag ttatggattt ggagcgttcg ggaaggattt tgcgatcggc attgtgtata
    19741 tgtacctcat gtattactac accgatgtcg tcgggctgtc tgtgggtttg gtcggtactt
    19801 tgtttctggt ggcgaggatc tgggatgcta ttaacgatcc gattatggga tggattgtaa
    19861 atgctacgcg atcgcgatgg ggtaagttca aaccctggat cctgatcggt acgttggcaa
    19921 actctgtaat cttatttctc ctctttagtg cgcatctgtt tgaaggtact actcagattg
    19981 tctttgtttg cgtgacctac atcctctggg gcatgactta caccattatg gatattccct
    20041 tctggtcgct ggttccaacc atcacgctcg ataaacgtga acgcgaacaa ctggttcctt
    20101 atccgcgttt ttttgccagt ctggcaggct ttgttacggc aggtgtgacg ctaccatttg
    20161 ttaattatgt cggcggtggc gatcggggat ttggctttca gatgttcact ctggtattga
    20221 tcgccttttt tattgtttca accatcatca ctctgcgcaa tgtgcatgaa gtcttttcgt
    20281 cagacaatca atcgtctgct gaaggaagcc atctgacact taaagccatc gttgcgctta
    20341 tttataaaaa cgatcagctt tcatgcctct tgggtatggc tcttgcttat aatgtagcca
    20401 gcaacattat taccggattt gctatctatt atttctcata tgttatcggt gatgcggatt
    20461 tgttccccta ttatctgtcg tatgcgggag ctgctaacct ggtgacgtta gtattcttcc
    20521 cacgcttagt taaatcatta tcccgacgca ttttatgggc cggagcatct attcttccgg
    20581 tgttaagctg tggtgttctc ctgttaatgg cattaatgag ctatcacaac gtcgtcctca
    20641 ttgtgattgc gggtattttg ctgaatgtgg gaacggcgct tttctgggta ttacaggtca
    20701 tcatggtggc agataccgtt gattacggtg aatataaact gcacgtacgc tgtgaaagca
    20761 tcgcttactc cgtgcagact atggtggtga agggcggttc agcctttgcg gcttttttca
    20821 ttgcggttgt gttagggatg attggctatg taccgaatgt tgaacagtct acgcaagccc
    20881 tattaggtat gcagtttatt atgattgctc taccgactct gtttttcatg gtaacgctga
    20941 ttctctactt ccgtttctat cgcctcaatg gcgacacgct gcgcaggatc cagattcatc
    21001 tgctggataa atatcgcaaa gtaccgcccg agcctgttca tgctgatatt ccggtcggtg
    21061 cagtgagtga tgtgaaagcc tgacgtggac aatagctaac gaaaatggcc tgatgcgata
    21121 tgtttatcag gccacgtctt aatgtaatgc tttggatttg caaattttgt agaccggata
    21181 aggaattcac gccgcatccg gcatcaacaa agtgcacgtt gttatccagt tatcaagcca
    21241 tagcgccgta gctggcggca atgaaagcga gcgagcaata tcactgtcgt taatgcgcca
    21301 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt accgagaaaa
    21361 tcatccgctc cctggcccat cgccagtgcc gcccaccact cgattaattc atacatcgcg
    21421 cttatcgcca gcgccacgca acagaccaga aacgccacca ttttacgtcc gcgcacgtac
    21481 atcccgcgaa cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaagtgc
    21541 cccagcttgt catacggatt acgactcaac cccagccatt cctgcacctc aaaaccaacg
    21601 gggactttcg cgtaggtgta ctgcccgcct accatcagga tgatggcgtg aaagaagatg
    21661 agcgtataga gcagcagcgt taacgggtaa cgtctggcgg tggcaagtag tagcggcacg
    21721 acgataatca ccggtgtcac ttccagcagc caggtgagtt tgtcatgggc cgaaatgccg
    21781 gtataaatca ggattaacgt tagcgccagt gcgccagtgt taagaattaa cggcttgagt
    21841 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
    21901 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
    21961 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
    22021 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
    22081 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
    22141 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
    22201 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
    22261 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
    22321 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
    22381 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
    22441 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
    22501 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
    22561 cggcctgacg cagttcttgt ggaatgtact gacctagatt gtgttccagt ttttcgatcc
    22621 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
    22681 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
    22741 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
    22801 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
    22861 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt gttttccgca gacgtaccgc
    22921 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
    22981 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
    23041 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
    23101 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
    23161 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
    23221 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
    23281 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
    23341 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
    23401 gaatagccgc aacctgtttc tggtgtgccg gacgtagcat gaaagaagcg tcgtgaaagg
    23461 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
    23521 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
    23581 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
    23641 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
    23701 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
    23761 caaccccggc agaataaaac tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
    23821 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
    23881 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
    23941 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
    24001 aatggcatac gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
    24061 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
    24121 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
    24181 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
    24241 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
    24301 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttatccag
    24361 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
    24421 gaactcttcg tctttatcca gatctttacc gcggaaccag ctgaagatac cgatcgccag
    24481 gataccgatt aacgtcgatg gaatggtgat tgccagcaga tcgaggaact caagatggcg
    24541 accatcaaag gtgacattac ccagcatcgc aaccagagac acgaccgcaa ccgacaccgg
    24601 actggcgata atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
    24661 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
    24721 cgtaccgcaa agaatggtca gtgtacaggt cacaaacggc gcgacaattg agacatattt
    24781 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
    24841 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caactggtgg
    24901 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
    24961 acccagcgcg ataccaccct ttctggcacc ataaaacaga catattagta ttatgataag
    25021 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
    25081 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
    25141 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
    25201 aaatttatgc actgtgataa gcggcttttt cagagagaaa aactctctgg cgatgggttt
    25261 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
    25321 gtctattttt tgttctcagg gcagatatca ttaggtttat agattaatct gatctaccca
    25381 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttatcg
    25441 ttttgaaatt aaaaacaccg ttcatctgaa gagatattaa tttttaacga tgatggaggg
    25501 ataattatat ttgatctggc acaagtttta ctgattaacg atgtaacttg tgccaggggt
    25561 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
    25621 ttgtttcagc agtgagtagt gttctgcctg aatacggtaa cggtaaaccg gacgccccgt
    25681 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
    25741 tcggcaggaa acacgcgaga tgttaacctc gttagccagc tcgtcggttg aaaactcata
    25801 gtcctgatgc gcgtcaatcc actggcacag cgtgcgtaac gtctgcggcg ttaagccttt
    25861 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
    25921 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agccggtgag
    25981 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
    26041 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
    26101 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
    26161 atcgagcaat atcaggtcga taggcgcatc gctattgaag ataatctctt tggctttctc
    26221 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
    26281 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
    26341 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tccccggttc cgattccacg
    26401 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
    26461 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
    26521 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
    26581 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
    26641 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
    26701 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcggt taatcttgct gattaaaaaa
    26761 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
    26821 ttggctgttt tgagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
    26881 agaatcacat gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcagcatag
    26941 ttgaccagac cgtcgagtcg ctgcatcagt ttacgaactt cagttttgtc cctgaaggtt
    27001 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
    27061 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
    27121 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
    27181 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
    27241 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
    27301 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
    27361 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
    27421 ctgtcattga tctgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
    27481 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
    27541 cgattgatag cgacattttc ttcgccattc agcgcattaa ggatgtcatc acctttaaat
    27601 ggctgaccaa tacgctgggc ttcaggatgt gagtagcgaa gactttgcat atcggtaacg
    27661 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccactc
    27721 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agtcggcgag ggtacgcgcc
    27781 actgccagtg ccttgttggc cagcccgtct cgcgtcatat cactgatttg cgagaagtaa
    27841 atcagatgca ccaccaatag caccgagaac agtaccgcgc tgaccattaa gatcacagtg
    27901 gtactcaatt tcatcggacg tttgcgtaat atgtggtagg gcaatgaatg tctcatcagc
    27961 ttccttgtgt gacaaatttc ttaagcatta tctctgatga ggcgggtaat
//
LOCUS       DAJTUP010000047        27925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTUP010000047 DAJTUP010000000
VERSION     DAJTUP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.047"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            554..1324
                     /locus_tag="OO468_002066"
     CDS             554..1324
                     /locus_tag="OO468_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR6259182.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1315..1788
                     /locus_tag="OO468_002067"
     CDS             1315..1788
                     /locus_tag="OO468_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR6259183.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1895..2434
                     /gene="dnaT"
                     /locus_tag="OO468_002068"
     CDS             1895..2434
                     /gene="dnaT"
                     /locus_tag="OO468_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR6259184.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2437..3174
                     /gene="dnaC"
                     /locus_tag="OO468_002069"
     CDS             2437..3174
                     /gene="dnaC"
                     /locus_tag="OO468_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR6259185.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3223..3717
                     /locus_tag="OO468_002070"
     CDS             3223..3717
                     /locus_tag="OO468_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR6259186.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3957..6248
                     /gene="opgB"
                     /locus_tag="OO468_002071"
     CDS             3957..6248
                     /gene="opgB"
                     /locus_tag="OO468_002071"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR6259187.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6386..7408)
                     /gene="lgoD"
                     /locus_tag="OO468_002072"
                     /pseudo
     CDS             complement(6386..7408)
                     /gene="lgoD"
                     /locus_tag="OO468_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7547..8461
                     /locus_tag="OO468_002073"
     CDS             7547..8461
                     /locus_tag="OO468_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6259188.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8676..10037
                     /locus_tag="OO468_002074"
     CDS             8676..10037
                     /locus_tag="OO468_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259189.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10086..11750)
                     /gene="tsr"
                     /locus_tag="OO468_002075"
     CDS             complement(10086..11750)
                     /gene="tsr"
                     /locus_tag="OO468_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR6259190.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11869..12315)
                     /gene="hpaR"
                     /locus_tag="OO468_002076"
     CDS             complement(11869..12315)
                     /gene="hpaR"
                     /locus_tag="OO468_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR6259191.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12587..13876
                     /locus_tag="OO468_002077"
     CDS             12587..13876
                     /locus_tag="OO468_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR6259192.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13873..15339
                     /gene="hpaE"
                     /locus_tag="OO468_002078"
     CDS             13873..15339
                     /gene="hpaE"
                     /locus_tag="OO468_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR6259193.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15341..16192
                     /gene="hpaD"
                     /locus_tag="OO468_002079"
     CDS             15341..16192
                     /gene="hpaD"
                     /locus_tag="OO468_002079"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR6259194.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16202..16582
                     /locus_tag="OO468_002080"
     CDS             16202..16582
                     /locus_tag="OO468_002080"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR6259195.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16650..17453
                     /gene="hpaH"
                     /locus_tag="OO468_002081"
     CDS             16650..17453
                     /gene="hpaH"
                     /locus_tag="OO468_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR6259196.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17464..18267
                     /gene="hpaI"
                     /locus_tag="OO468_002082"
     CDS             17464..18267
                     /gene="hpaI"
                     /locus_tag="OO468_002082"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR6259197.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18289..19644
                     /gene="hpaX"
                     /locus_tag="OO468_002083"
     CDS             18289..19644
                     /gene="hpaX"
                     /locus_tag="OO468_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR6259198.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19654..20544
                     /gene="hpaA"
                     /locus_tag="OO468_002084"
     CDS             19654..20544
                     /gene="hpaA"
                     /locus_tag="OO468_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR6259199.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20571..21508
                     /locus_tag="OO468_002085"
                     /pseudo
     CDS             20571..21508
                     /locus_tag="OO468_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21735..23293
                     /gene="hpaB"
                     /locus_tag="OO468_002086"
                     /pseudo
     CDS             21735..23293
                     /gene="hpaB"
                     /locus_tag="OO468_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23311..23823
                     /locus_tag="OO468_002087"
     CDS             23311..23823
                     /locus_tag="OO468_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR6259200.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24216..26366
                     /gene="btsT"
                     /locus_tag="OO468_002088"
     CDS             24216..26366
                     /gene="btsT"
                     /locus_tag="OO468_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR6259201.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26416..26619
                     /locus_tag="OO468_002089"
     CDS             26416..26619
                     /locus_tag="OO468_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6259202.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26630..27586
                     /gene="yjiA"
                     /locus_tag="OO468_002090"
     CDS             26630..27586
                     /gene="yjiA"
                     /locus_tag="OO468_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR6259203.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27655..>27925)
                     /locus_tag="OO468_002091"
     CDS             complement(27655..>27925)
                     /locus_tag="OO468_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6259204.1"
                     /translation="NEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKS
                     IYEKLNSKSSFYLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6800 a         7329 c         7054 g         6742 t
ORIGIN      
        1 tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt catatcagca
       61 tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca aatgataacc
      121 tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa atacttaatg
      181 aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc cgcagaatgc
      241 gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt gttaacacta
      301 aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata aattcattat
      361 agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt tttatcaccg
      421 agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta ccgcaaaaca
      481 accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc acgtctatga
      541 caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt gtatccagtg
      601 tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc tttcctcacg
      661 actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga acgccatagt
      721 gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc acgatcgcgg
      781 cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg agcatcatct
      841 gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac gttacccaag
      901 atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac tcaataacgg
      961 tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt atatccgcca
     1021 gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg ctttcgccgc
     1081 caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc ccaccattgc
     1141 gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg ccgtcgccga
     1201 tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca gtctgctgac
     1261 actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac gcggatgggt
     1321 gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat tcctgcggtc
     1381 ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc gctgcttggc
     1441 tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat tgagtggtca
     1501 acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg ctggtatctg
     1561 gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg catatcggct
     1621 tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga accgttgatg
     1681 attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt atccatcggt
     1741 ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa ttgcctcgct
     1801 taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa agttatctcc
     1861 cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt tgaccccgga
     1921 cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa aagctgaagg
     1981 cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca cgcctgcacg
     2041 cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg atgtcgctct
     2101 ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca tggggaaatt
     2161 cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg cgctatgggg
     2221 cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg cctactggca
     2281 ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc gcagcctgca
     2341 aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg tcagtgaacc
     2401 tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg cgacctgatg
     2461 caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac gggtgaagag
     2521 ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga acgtgaaaat
     2581 cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact gcatcagaac
     2641 tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt aagcaaagcg
     2701 cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc tggtaagcca
     2761 ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct acgcggtaaa
     2821 tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac cttcaggaat
     2881 agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct gctggtgatc
     2941 gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa ccagatcgtc
     3001 gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa tatggaagag
     3061 atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa cagtttatgg
     3121 gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta ttaatccccg
     3181 tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa aactattaaa
     3241 catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt gcatgctgcg
     3301 tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa cagcacgaca
     3361 caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg aaaccaagcc
     3421 ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc gaagcaaaag
     3481 ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa gctgggcctg
     3541 aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg tttgctgaaa
     3601 acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct ggcagaaaag
     3661 gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat ttcctgaacc
     3721 gcgaaatgtt acccggctga cgcggctctc tcctgccctt ttccctgctc ctggacggtt
     3781 ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa actaattcct
     3841 aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc attacattgt
     3901 gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat cgcccattgt
     3961 cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca tggaaagcgg
     4021 gacgtaacac ctggtggttt gcagccacgt taacggtgct ggggctattt gtcgttttaa
     4081 atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac gcggttctct
     4141 ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg ccgggtatcg
     4201 gcattgtgct ggggctgacg gcggtgttcg gtgcgctggg ctggatcctg cgccgtcgtc
     4261 gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg ctgggttcgg
     4321 tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag tcacgcgatg
     4381 gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct gacccgaaac
     4441 tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat aacgaagctt
     4501 tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat ttcagccaca
     4561 cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct cagtgcggca
     4621 taccgctgtt cgcccccttt gaaggcaacg cctccgcctc tgtctccagc ttcttcccgc
     4681 agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat ttcgtgcagg
     4741 gcgcaaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc ttcgaccact
     4801 tatacggctc agaagagctg aaaagcgtgg tagccgaccc gcactatcgc aacgactggg
     4861 gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag ctttcccgct
     4921 caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg gatggtttta
     4981 tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat cagtcattca
     5041 gcgcggtaag ttgcagccag gagaatatcg cgacgtttat caacaaaatc aaagcgtcac
     5101 cgtggtttaa agatactgtc atcgtcgtct cttctgacca tttagcgatg aacaacacgg
     5161 cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt cgtggcgaca
     5221 agccgcagca aaagacgctg gcagtgaagc gtaacacgat ggataacggc gcgacggtgc
     5281 tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta tccgggcagt
     5341 cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag ccagatatca
     5401 tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag cagaaaaaca
     5461 tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt tcagacaaac
     5521 gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag ctggccgatt
     5581 tcgctccacg cgacaatttc gtctgggttg acagttgcta caagatggca caactctggg
     5641 ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt ggcggtcagc
     5701 aaattgttca gcatgttgac aaaacaacat ggaagagcaa gacggcattt aaagatacgg
     5761 tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt gataacgata
     5821 ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa gaggtgaaac
     5881 agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg cagctgggcg
     5941 atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg gtgattaccg
     6001 ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc aatgaagaac
     6061 aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat aacccaaccg
     6121 atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa gggaatatcc
     6181 tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg gtagaacgtg
     6241 aagggtaatt cctgactgat gtgtaggtcg catagggtgc gacctacacg ccttctccgg
     6301 cttacaacac attattaaga aacaaaaaaa acagcgccca acacttcccc ggacgctgca
     6361 tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc aactcacgat
     6421 tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag cgatgggtta
     6481 acatcatgtc ggcagtgatt ttcccttccg ccatcagtcg accgacttta gcgaaatctt
     6541 ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat tccggatcgg
     6601 agaactgcaa ctcaccttta aacagaccga caaataccac cgtgccgccg tgacgaatca
     6661 gattcacggt attattcatc gcatgttgat tacctgtcgc gtcgatcact ttctgcgcca
     6721 gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac gggtccagca
     6781 cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta tccgccacca
     6841 ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt ggccccgccc
     6901 cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca tgagcgctaa
     6961 tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg ggcaaaatgt
     7021 tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg ccaatgactg
     7081 aaattttttc acagcagttg gtacgcccgc ttttacacgc cgggcattgc tgacaggcaa
     7141 cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt ttacccagcc
     7201 caacaatctc cccacatatt tcatggccta aaacccgtgg ataactaaaa aatggttgat
     7261 ttccacccta ggcatgaata tcggtaccgc aaatcccgac agacttaatt tttattaatg
     7321 cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct ttcggctgct
     7381 ggcaaattaa aacattcatc gtagacatga ttcggctatc cattttttgt tgccttagat
     7441 atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa acacacgcat
     7501 aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga gtcgttcaca
     7561 aaatttacgc cacaatgtga ttaaccaggt cattgatgat atggcgcggg gtcatatccc
     7621 ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc gcactacagt
     7681 gcgtcatatt ctcagccaat tacgcgaatg cggcgtctta acgcaggttg gaaacgacta
     7741 tgttattgcg cgtaaacctg accatgatga cggtttcgcc tgtactaccg cttcaatgag
     7801 tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc gtcagttacg
     7861 ccctagggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca gcccggtggt
     7921 tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg aaaagcgcgg
     7981 ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg agctacgcga
     8041 aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg atccccgctg
     8101 gctgcaggcg aaaaccatgc tagaacgtca tcggctactg cgtgacaaca tcggcaatag
     8161 ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt cagctgccga
     8221 caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt ttcactatca
     8281 atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgaac atatgaccat
     8341 tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc gtaaccattt
     8401 gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt atgctcatta
     8461 agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa agataaatac
     8521 cgggaaattc ccgcttacct atgctcacaa tcagtcgact aaaaatgttt ttgtgagcgg
     8581 tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat gcacaattac
     8641 gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat atcaccctcg
     8701 atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca ccagatggat
     8761 tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg ttattatttt
     8821 ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat ttaacgattc
     8881 gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg ttttcactcg
     8941 cttacggcat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc ccacgcctga
     9001 tgctgggact ggggatgttc ttctggtcac tgttccaggc catgtctggc atggtgcaca
     9061 gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg ccgatgaacc
     9121 catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga cgcccgatgg
     9181 gcttcttcaa cgcagcttct accattggcg ttgctgtaag cccaccgatt ctggcggcaa
     9241 tgatgctggt gatgggctgg cgcgggatgt ttattaccat tggtgtactg gggatttttc
     9301 tcgccatcgg ctggtatatg ctctatcgca accgcgagca cgtagaactg actgccgttg
     9361 aacaagctta tctcaatgca ggtagcgtca atgcccgccg agatccgctc aattttgccg
     9421 aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga ttcagtggca
     9481 tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa acagcctata
     9541 acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt ggggctgccg
     9601 ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg gctccgatta
     9661 aaagccgtaa gatctgcatt attaccggga tgttctgttc tgccgccttt acgctgatag
     9721 taccacaagc gacaacatcc atgatggcgg ttctgctgat tggcatggca ctgttctgta
     9781 ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct tctcgcatga
     9841 ctgcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct ttcgcgccga
     9901 tcattactgg ttttattgtt gatacaaccc actcattccg tctggcacta atcatttgcg
     9961 gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgccag ccgatcaacg
    10021 acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg gcatttttat
    10081 ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt acgcggcgta
    10141 gctggcgtca cggtttttac cacagccgat gtttcacgct gctgttgctg ctgttgctga
    10201 atccggaaca ctgccacagc ttcggtcagg cgactggcct gctcttccag cgcggcggcg
    10261 gcagcggcag attcttccac cagcgcggcg ttctgttgag ttacccggtc catctcagca
    10321 accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga agctatttcg
    10381 cccataatgt ccgtcacgcg ggtcacagca ctgacaatct ccgccattgt ttccccggcg
    10441 ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat caggctttta
    10501 atttcacgaa ccgcctgggc gctacgctgg gccagattac gcacttctcc cgcaaccacc
    10561 gcaaaaccac gtccttgctc acccgcacgc gctgcttcaa ccgccgcgtt caaagccaga
    10621 atattggtct ggaaggtaat gccgtcaatt acgctgataa tatcggcgat tttctgcgaa
    10681 ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc gccgcgttgc
    10741 gccgtttcag aagcacttaa tgccagatgg ctggcctggc gcgcattctc ggcgttctgt
    10801 ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga agcggcctgt
    10861 tgctcggtgc gcgaagagag atcattattg ccggtagcga tttcgctggc accgctatag
    10921 atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc ctgcatatgg
    10981 cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat cggtttcacc
    11041 agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc gattcattgg cgctaccagc
    11101 gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat catcacgccc
    11161 accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc gatatcatag
    11221 agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc ctgatatccc
    11281 tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg gatcagctcc
    11341 gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc ggcggtgctc
    11401 tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc ctgtttcagc
    11461 gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat attattctga
    11521 tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa caacgcgacc
    11581 cagctgccat tcagcgtggg ttgctgctgg cgaatggttt gtaaaacagt gaaattttct
    11641 ttgtcattct ttaaggcatt aaaaaacaga ccgcctgatg tcagttgtaa aaggccaaaa
    11701 acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat ggtttctctt
    11761 tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga aaaggtgtaa
    11821 cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt attcattatc
    11881 gcccgggatg tcttcttgtc gggaattgcc cagagcaata aattcctcta acagatgggt
    11941 taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg cttcttcaat
    12001 ctgtgtctga gcgcggttat acagcgcctg cccctctttg gtcagcgaga tatagagctt
    12061 ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca tccgcgtgag
    12121 gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt gaaaatccat
    12181 tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca aattatgccg
    12241 tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca acgcaatggt
    12301 tagtgagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat agaaacaatt
    12361 aatcatcacg ttaccgggac tgagctgccc ccgcaattct tcattttatc aaaattgatg
    12421 atattttatt aacttactga tatttaatat tttattatct ttaaatgtaa atagtttgtt
    12481 aattagatca catttatatc acttactttt gccaaactac gcaatcggga ataaaagtaa
    12541 tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga aaggcactat
    12601 cttcgccgta gcgttgaacc atcgcagcca acttgatgcc tggcaggaag cgttccagca
    12661 atctccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc gcaatacggt
    12721 gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaagtactga gcggtgcgac
    12781 tgttgcgctg attgtgggga aaacggcgac gaaagtacgt gaagaagatg cggcagagta
    12841 catcgccgga tatgcgctgg ataacgacgt cagcctgccg gaagagagct tttaccgtcc
    12901 ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg tggctctcag
    12961 caatgtcgat aatctgacta tctataccga gatcaacggg cgtcctgccg atcactggaa
    13021 taccgccgat ttacaacgta acgccgcaca gctgctgagc gccctgagcg aatttgccac
    13081 gctgaatcca ggcgatgcta ttctgctcgg cacgccacag gcgcgcgtgg aaatacagcc
    13141 aagcgatcgc gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc cggtagtgga
    13201 cgaacgtgaa gtgaccacgc gcaagagctt cccaacgctg ccacacccgc acggtacgct
    13261 gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta agccaccgga
    13321 agagccgctg gtgttcctga aagcgccgaa taccctcact ggcgacaacc agacctccgt
    13381 gcgcccgaac aatattgaat acatgcacta cgaagcggag ctggtggtag ttattggcaa
    13441 gcaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct acaccgtgtg
    13501 taacgactac gccattcgcg actatctgga aaactactac cgccctaacc tgcgggtcaa
    13561 aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg cgatcccgga
    13621 cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc aaggcaccac
    13681 cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat ttatgaccct
    13741 gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg tggtgcctgg
    13801 cgatgaagta gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa ttgtgagtga
    13861 ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt tgcaggtaac
    13921 gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt ggcctctggc
    13981 ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc gaaatgggct
    14041 gatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct gatcgaccag
    14101 aacgtgcctg agatcgccgc gatggaaacc gcggacacgg gcctgccgat ccatcagacc
    14161 aaaaatgtgt tgatcccacg cgcttctcac aactttgaat ttttcgcgga agtctgccag
    14221 cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac gctggtgcaa
    14281 ccggtaggcg tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac cgccacctgg
    14341 aaggtcgcgc cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc ggaactctcc
    14401 ccgctgaccg ccgaccgcct gggtgagctg gcgctggaag ctggtattcc ggcgggcgtg
    14461 ctaaacgtgg tacagggcta cggcgcaacc gcaggcgatg cgctggtccg tcatcatgac
    14521 gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc gcaacatcat gaaaaacgcc
    14581 gggctgaaaa aatactccat ggagctgggc ggtaaatcac cggtgctgat ttttgaagat
    14641 gccgatattg agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat caacggcgaa
    14701 cgctgcaccg ccggttcgcg catctttatt cagcagagca tctatccaga attcgttaaa
    14761 cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc gaatacccag
    14821 gttagggcgc ttatcagtca gcaacactgg gaaaaagtct ccggctatat ccgtctcggc
    14881 attgaagaag gcgcaaccct gttggcgggc ggcccggata aaccgtccga cctgcctgcg
    14941 cacctgaaag gcggcaactt cctgcgccca acggtgctgg cggacgtaga taaccgtatg
    15001 cgcgttgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt taaagacgaa
    15061 gccgaaggct tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta catctggaca
    15121 caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat ggtgttcgtc
    15181 aacacccaga acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc ctccggcacc
    15241 gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa cgtctgcatt
    15301 tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt tagcgttagc
    15361 cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa aaaaccacgg
    15421 ttgtcgccag ggtgcgatcg acgggcataa agagatcagc aagcgttgcc gggaaatggg
    15481 cgtcgatacc attatcgttt tcgatactca ctggctggtc aacagtgctt atcacatcaa
    15541 ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctgccgcatt ttattcgcga
    15601 catgacctac aactacgagg gcaacccgga gttggggcag ctaattgccg aggaagcctt
    15661 aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg aatacggcac
    15721 gctggtaccg atgcgctaca tgaatgaaga caagcacttc aaagtggtct ccatttcggc
    15781 tttctgcacg gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga ttctgaaagc
    15841 gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat cgcaccgctt
    15901 tattgacgat cagcgtgcgg aagaagggat gaacagctac acccgcgagt tcgaccgcca
    15961 gatggacgag cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt tttgcaatat
    16021 gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca cggtgatgct
    16081 gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatta ccgagctgtt
    16141 cccaagctct ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt aaggacgttt
    16201 tatgccgcac tttatcgtgg aatgtactga aaatattcgt gaagaagccc gcttacctga
    16261 gctgtttgcc agtgttaaca ctgcactggc ggcaacgggg atttttccgc ttgggggaat
    16321 acgtagccgg gcgcactgga tcgatacctg gcaaatggct gatggcaagc acgattacgc
    16381 ctttgtgcat atgacgctaa aaatcggcag cggacgcagt ctggaaagcc gtcaggaagt
    16441 gggcgaaatg ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg agagccgtta
    16501 cctagcgctt tcatttgaga ttgcagagct gcacccgacg ttgaatttca aacagaacaa
    16561 cgtacacgcg ttgtttaaat aaatttgcac cagcttccag gccggatgat aaggcgctgc
    16621 cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac accctgatcg
    16681 cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc tcgctggatt
    16741 acccggagat caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt cgcctgaaaa
    16801 tcgccgaaag tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa gcgatgcagg
    16861 caagctcgca gatcagcgaa ccggattacg gtgcactgct ggacgacatg ttcttccacg
    16921 atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg gagctggctt
    16981 ttgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt tacaacgcca
    17041 cggactatgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac atcgatccgg
    17101 aaacccagcg cccgcgcaaa gtattcgaca ccatttccga taacgccgcc aacgccggag
    17161 tgatcctcgg aggtcgtccg atcaaaccag acgaactgga tctgcgttgg atctccgccc
    17221 tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg ctgaatcatc
    17281 cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta caactggaaa
    17341 ccgggcaaat cattctcggt ggttcgttca ccctcccggt tccggcgcgt aagggcgaca
    17401 ccttccacgt cgattacggc aacatgggct ccattagctg ccgctttgtt taaggagaga
    17461 acgatggaaa acagttttaa agcggcgctg aaagcaggcc gtccgcagat tggattatgg
    17521 ctggggctga gcagcagcta cagcgcggag ttactggccg gaccaggatt cgactggtta
    17581 ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca actacaggcg
    17641 attgcgccct accccagcca gccagtggta cgcccatcgt ggaacgatcc ggtgcaaatc
    17701 aaacaactgc tggacgtagg cacacaaacc ttactggtgc cgatggtaca aaacgccgac
    17761 gaagcccgta aagcggtacg cgccacccgt tatccccccg ccggtattcg cggtgtgggc
    17821 agtgcgctgg ctcgcgcctc gcgctggaat cgcattcctg attacctgca aaaagccaac
    17881 gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa cttaccgcag
    17941 attctggacg tggaaggggt cgacggcgtg tttatcggcc cggcggatct gagcgccgat
    18001 atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga gcaggcgatc
    18061 gtgcagatcc gcgaagcggg caaagcgccg gggatcctga tcgccaatga gcaactggca
    18121 aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac caccctgctc
    18181 gcccgcgccg ccgaagcgct ggcagcacgg tttggtaata gttcttctat ttcgatggcg
    18241 aagcaaaaca acaactctgt ctactaaacg tacgatgtgg agcgcatcat gacgacctct
    18301 accctgcaat ataaagataa taaaactgtt gaaattgaac atcgtgtcat taataaatta
    18361 tttcgtcgcc taatcatttt tctttttatt ctgtttgttt tttcgtttct tgatcgcatc
    18421 aatataggat ttgccggact gacaatgggc aaggatcttg gcctgacgtc cacaatgttt
    18481 ggcctcgccg caacactctt ttatgtcacg tacgtcatgt gcgggatccc cagtaatatg
    18541 atgttagcga aaattggcgc tcgtcgatgg atcgctggga tcatggtggt ctggggaatt
    18601 gcttccacct gcacaatgtt tgcaacaagc cccgaaacac tctatctact gcgtatgctg
    18661 gttggtattg ctgaggcggg ttttttacct ggtattttgg tttatctgac ctggtggttc
    18721 ccggcatatc atcgggccag agccaatgcc ttatttatga tagcgatgcc agtcactatg
    18781 atgctgggat tgatcctttc aggctacatt ctggccttgg atggactgtg gaatctaaaa
    18841 ggctggcaat ggctcttttt gctggaaggt ctgccatcgg tcattcttgg tgtcgtcacc
    18901 tggttttatc tcaacgacac accagaccag gcgacctggc ttgatgaaaa cgaaaaacag
    18961 gctttaaaaa cgatgattct ccgtgaacaa gagagtgcat ttgcccatgc atctgttcca
    19021 cgctcaacct tacgtgaagt cctgacccca gccatcatgc tatatacact tgcttatttt
    19081 tgcccgacta atactctgag tgcgattaat atctggacgc cgcaaatcct acagagtttc
    19141 aatactggca gtagcaacat catgattggt ttgctggcgg ccatccccca attctgcaca
    19201 atttttggca tgatctggtg gagtcgtcgt tccgaccgtc ttaaagaacg caaaaagcat
    19261 acgattcttc cctacctgtt tgccgctgcc ggatggttac tcgcttctgc gacagatcac
    19321 agcctcattc aactgagcag catcattatg gcctcaatgg gatcatttac cgcgatgact
    19381 atcttttgga caacaccgga tcaagttata agcttgcaat cgcgtgccgt tgcgatggct
    19441 gtcatcaatg ctgtcggcaa tgtaggttca gcagtcagtc cattacttat cggtattttg
    19501 aaagatgcaa ccggcagttt tagctcaggt ttgtggtttg tagcaggatt gctggtactg
    19561 ggcgcgttag tcatgacccg aatccctatg gggagtcggg aacactctct ttcatctgaa
    19621 cttatcgcac caaaaccgca ataaggagtc atgatgtcca gtgaagctat ctttgaacat
    19681 ggttatgaaa atattgattt ggtccgggaa tatgatgccc gctacgctgg tgaagatgtg
    19741 cattatgaaa catttgcccg gttagcgaat ttctttggac gcgatatgcg ccctcactgg
    19801 cacgatagat attttcaact ccattatctg gtcactggac gcattacttt acaactggat
    19861 gagcacttct attctgtgcg cgcaccgctt tttgtactaa caccaccatc ggtgccacat
    19921 actttcttta ctgatcctga tagcgatggg catgttctga ccgtacgtca ggagttaatt
    19981 tggcccctgg tagaaaagct ctggcctggt agtggagatg ccgcaatgct acaaggtatc
    20041 tgtctgtcac tggagggaat ggaacataca ctggcggcat taaatcatta ttggccgata
    20101 atatccctgg agtttcaaca aaataagcaa ggacgggaaa ttctattgca atcactggcg
    20161 caaagtattt ttacaatcct actgcgtgaa gcaccaccaa atgatatttc aacatgcagc
    20221 gtgcgcggcg agatgtgttt attccagaag tttaatcggc tgattgacga aaattatcgc
    20281 caacatttaa tggtccctga atatgcaaca atgctcggta tgagtgaatc acggcttacc
    20341 gagctgtgcc gacgttttgc caatcaatcg ccgaagagat tgattttcga acgagtgagc
    20401 cgggaagcca gacggcttct gctctatagc gagcaaagca tcaaccagat agcccttgat
    20461 cttggctaca aagatcccgc ctatttcgcc cgttttttca accgaatgat gggttgctca
    20521 ccaacacaat tccgtggtcg ctgaccattt atcaacctga gagactcgac atgaaaatat
    20581 ctttacgctc actatttgtg gcgtgcctga ttgcgccagt ggcattcacc tacgcctccg
    20641 aaccgcaata aatgaaaatt gtactacttg gaacgaaagg tggcccttct ttattaaaag
    20701 ctgaccgttt accgatggca acggcactta ttattggcaa taaaacctgg ttaattgatg
    20761 ctggttacgg ggccagctta caactggtaa aaaataatat tccactgcgc cagattgata
    20821 atattttact cacccattta cacagtgatc atattctgga ctatccatca gtcttaatga
    20881 atgcatgggc tagcggtctg caggacaaaa ctattcatgt attcggtccg ccaggcaccc
    20941 gagccatgac cgctgccagt tggcaggtct tcgatcggga catcacactg cgtatggaag
    21001 aagaaggaaa acccgatcct cgcaaactcg ttacagctca agatgttggt gaaggcgtag
    21061 tttataaaga tgataccgtg accgtgactg cattaaaggt ccctcattct cctttccctg
    21121 acggtgaggc ttttgcttat cgcttcgaca cacaaggcaa gcgaattgtc ttctcaggag
    21181 atacctcgta ctttccccca ctggcaactt tctcagaagg cgctgatata cttgtgcatg
    21241 aggccgtaca tgagccctcc gtcgctaagc tggcagacag tattggcaat ggtaaaacac
    21301 tggcaaaagc cattgcttct caccatacga ctattgagga tgtaggcaaa attgccaatg
    21361 aagcaaaagt gaaaaaactc atccttagcc acctggtacc aatcaccgtt ccggatgaaa
    21421 catggaagca agaagcacag aaaactattc aggtcctatc gtcgttggtc atgaaaatat
    21481 gacgattacc attccattcg ccccataaaa aattccgtcc ccgttagccc ttgttagcgg
    21541 ggaatattat gatttgcctt actgtaagtt gatcacgatc gcagtttact atttaactcc
    21601 accttgctgt cgaaaagtac caacgttcgc ctgaaacgtc tcttcccgac aaccgcgttc
    21661 aacgcttcaa ttaaataaca aaaacaacac acaaaattaa caatcgtatt ccgattaata
    21721 ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt cctttcaccg
    21781 gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat ggcgagcgag
    21841 tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt gcccaactgt
    21901 acgacgcgct gcacaaaccg gagatgcagg actctctgtg ttggaacacc gacaccggca
    21961 gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac ctgcgccagc
    22021 aacgcgacgc catcgctgaa tggtcacgcc tgagctatgg ctggatgggc cgtaccccag
    22081 actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac ggtcagttcg
    22141 agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac tttaaccacg
    22201 cgattgttaa cccaccgatc gtcatttgcc gaccgataaa gtgaaagacg tttacatcaa
    22261 gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg ttgccaccaa
    22321 ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtga tgggcgaaaa
    22381 cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga aattaatctc
    22441 ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg ccatacgact acccgctctc
    22501 cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtactga tcccatggga
    22561 aaacgtgctg atctaccgcg attttgatcg ctgccgtcgc tggacgatgg aaggcggttt
    22621 cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg acttcattac
    22681 ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg gtgtgcaggc
    22741 cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg actcgatgtg
    22801 ttcagaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg cactgcaaac
    22861 ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatag aacgcaacgt
    22921 taccagtggc ctgatctacc tcccttccag tgcccgtgac ctgaacaatc cgcagatcga
    22981 ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc agcgcatcaa
    23041 gatcctcaaa ctgatgtggg acgccattgg cagcgaattt ggtggtcgtc acgagctgta
    23101 tgaaatcaac tactccggta gccaggatga gattcgcctg caatgtctgc gccaggcaca
    23161 aagctccggc aatatggaca agatgatggc gatggttgat cgctgcctgt cggaatacga
    23221 ccagaacggc tggactgtgc cgcacctgca caacaacgac gatatcaaca tgctggataa
    23281 gctgctgaaa taacgcagca ggaggttaag atgcaattag atgaacaacg cctgcgcttt
    23341 catgacgcga tggccagcct gtcggcagcg gtaaatatta tcaccaccga gggcgacgcc
    23401 ggacaatgcg ggattacggc aacggctgtc tgctcggtca cggatacacc accgtcgctg
    23461 atggtgtgca ttaacgccaa cagtgcgatg aacccggttt ttcagggcaa cggcaagttg
    23521 tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcgc gccacttcgc gggcatgaca
    23581 ggcatggcga tggaagagcg ttttagcctc tcatgctggc aaaaaggtcc gctggcgcag
    23641 ccggtgctaa aaggttcgct ggccagtctt gaaggagaga tccgcgatgt gcaggcgatt
    23701 ggcacacatc tggtgtatct ggtggagatt aaaaacatca tcctcagtgc agaaggtcac
    23761 ggacttattt actttaaacg ccgtttccat ccggtgatgc tggaaatgga agctgcgatt
    23821 taagctccag ccccttcacg cattcgtcga aggggcattt ttattgtgat ctcaatcaca
    23881 tatctacccg atgaataatt cttgcccaac cgctcaccgc cattcctgac cgcttacatc
    23941 cctaaaataa ccactcagtt atttacctta ctttacgcgc acgtatctct ggcaacatca
    24001 ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa cggcttcggc
    24061 ctatgattaa caaattactc tatggatttt ggtttgcagg aaggcagcaa gtgagtgaat
    24121 ccccgggagc ttacaaaagt aagtgtcaga ggtgaacgaa cgcagctgcc gcacctgtaa
    24181 gccaaaagac gacgagtaaa atgccaggtt ttactatgga tacgaaaaaa ctattcaagc
    24241 acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta gttgcattac
    24301 gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtgtcg gtgtatctgg
    24361 tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc gaccccacgc
    24421 gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc aaccgttacg
    24481 tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg ggaccggttc
    24541 tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg gtagtgctgg
    24601 ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac ggcgcatctc
    24661 tcggtgagat gatcaaagaa gagatggggc cagtaccggg gactatcgcg ctgtttggct
    24721 gtttcttaat catgatcatc atcctcgccg ttctggcgct gattgtggtt aaagccctgg
    24781 ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg ctgtttatgg
    24841 gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtt attggtatcg
    24901 tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg tactggggtc
    24961 cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat gcgtttgttt
    25021 ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca accttcctga
    25081 aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg gaactgaaaa
    25141 tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc gctctgttcc
    25201 cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg ctgatctctt
    25261 ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt ggctacggcg
    25321 caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc atcatcgaac
    25381 cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc atgcctaacc
    25441 tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa gacgttaccg
    25501 cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag cagatcctgc
    25561 aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt gcgccgacgc
    25621 tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac atgggcttct
    25681 ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg gatgcgggta
    25741 cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg ttcctgaaaa
    25801 aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt ctgtggggct
    25861 acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg tggccgctgt
    25921 tcggtatctc caaccagatg ctggcagccg tagcgctggt actgggcacc gttgtgctga
    25981 ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta tggctgctta
    26041 tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac caacccgcag atggaaggct
    26101 tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac ctgacggcgc
    26161 agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca ggcctgagta
    26221 ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc tggcttgcgg
    26281 tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc ccggaaggcg
    26341 gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg ggctttgttc
    26401 ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct cggccaggcg
    26461 gcgaagatgt tgattggtat tccagactac gacaactacg tcgagcatat gaagaccaac
    26521 catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca gaacgcgcgc
    26581 tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca tgaacccgat
    26641 tgcagttacc ctactcaccg gttttttagg cgcgggcaaa actaccctgc tgcgccatat
    26701 tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg gcgaagtctc
    26761 cgttgatgac caactgattg gcgaccgcgc cacgcagatc aaaacgctga ccaacggctg
    26821 catctgctgt tcgcgctcca acgagctgga ggacgcacta ctcgacctgc tggataatct
    26881 cgacaagggc aatattcagt tcgatcgtct ggtgattgaa tgcaccggca tggccgatcc
    26941 cggcccgatt attcagacct ttttctccca tgagatttta tgccagctct acctgctgga
    27001 cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc agttcaccat
    27061 cgcccagtcc caggtgggct acgccgaccg tattctgctg actaaaaccg acgtcgcagg
    27121 cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac cggtctacac
    27181 cgtcacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt ttatgctgga
    27241 agagaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac aaaacgatat
    27301 ttcgtcaatt gtggtggaac tggattaccc ggtagatatc agcgaagttt cccgcgtgat
    27361 ggaaaacctg ctgctggaat cggcggataa actgctgcgt tacaaaggga tgctgtggat
    27421 tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca gcgccgactg
    27481 ggacaggccg tggggcgatg aaaaaccgca tagcacaatg gtgtttatcg ggattcagtt
    27541 gccggaggaa gagattcggg cggcgtttgc gggattgagg aagtaagttc gggaaagatc
    27601 aactttttga gagttccacc gcaacattat tacgagtgga actctttaga tatgtcagac
    27661 caaccaaagc ttcttcaatt gcgttttcat tttttcatgt ctcattttca gttcagccaa
    27721 tccccattct gaagaattac aaacatcttt tgttaaataa aaagagcttt tactattaag
    27781 tttctcataa atagatttct ttttgataaa atcatagtgt tgtgtttcag aatttttgga
    27841 accacttatg agtgttaaat tacctatttt atgcagccag tagacatgct cttgaggttg
    27901 gaatctatta atccagtatt catta
//
LOCUS       DAJTUP010000048        27775 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTUP010000048 DAJTUP010000000
VERSION     DAJTUP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.048"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(57..1349)
                     /gene="spaL"
                     /locus_tag="OO468_002092"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             complement(57..1349)
                     /gene="spaL"
                     /locus_tag="OO468_002092"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR6259205.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            complement(1353..1754)
                     /gene="spa15"
                     /locus_tag="OO468_002093"
                     /gene_synonym="spaK"
     CDS             complement(1353..1754)
                     /gene="spa15"
                     /locus_tag="OO468_002093"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR6259206.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            complement(1767..3827)
                     /gene="mxiA"
                     /locus_tag="OO468_002094"
                     /gene_synonym="virH"
     CDS             complement(1767..3827)
                     /gene="mxiA"
                     /locus_tag="OO468_002094"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR6259207.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            complement(3840..4907)
                     /gene="mxiC"
                     /locus_tag="OO468_002095"
     CDS             complement(3840..4907)
                     /gene="mxiC"
                     /locus_tag="OO468_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR6259208.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            complement(4926..6626)
                     /gene="mxiD"
                     /locus_tag="OO468_002096"
     CDS             complement(4926..6626)
                     /gene="mxiD"
                     /locus_tag="OO468_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR6259209.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            complement(6613..7245)
                     /gene="mxiE"
                     /locus_tag="OO468_002097"
     CDS             complement(6613..7245)
                     /gene="mxiE"
                     /locus_tag="OO468_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR6259210.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            complement(7375..7803)
                     /gene="mxiM"
                     /locus_tag="OO468_002098"
     CDS             complement(7375..7803)
                     /gene="mxiM"
                     /locus_tag="OO468_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR6259211.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            complement(7787..8194)
                     /gene="mxiM"
                     /locus_tag="OO468_002099"
     CDS             complement(7787..8194)
                     /gene="mxiM"
                     /locus_tag="OO468_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR6259212.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            complement(8187..8882)
                     /gene="mxiN"
                     /locus_tag="OO468_002100"
     CDS             complement(8187..8882)
                     /gene="mxiN"
                     /locus_tag="OO468_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR6259213.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            complement(8824..9351)
                     /locus_tag="OO468_002101"
     CDS             complement(8824..9351)
                     /locus_tag="OO468_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR6259214.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            complement(9348..10073)
                     /gene="mxiJ"
                     /locus_tag="OO468_002102"
     CDS             complement(9348..10073)
                     /gene="mxiJ"
                     /locus_tag="OO468_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR6259215.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            complement(10079..10372)
                     /gene="mxiI"
                     /locus_tag="OO468_002103"
     CDS             complement(10079..10372)
                     /gene="mxiI"
                     /locus_tag="OO468_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR6259216.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            complement(10385..10636)
                     /gene="mxiH"
                     /locus_tag="OO468_002104"
     CDS             complement(10385..10636)
                     /gene="mxiH"
                     /locus_tag="OO468_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR6259217.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            complement(10644..11759)
                     /gene="mxiG"
                     /locus_tag="OO468_002105"
     CDS             complement(10644..11759)
                     /gene="mxiG"
                     /locus_tag="OO468_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR6259218.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            complement(11768..12226)
                     /gene="ipgF"
                     /locus_tag="OO468_002106"
     CDS             complement(11768..12226)
                     /gene="ipgF"
                     /locus_tag="OO468_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR6259219.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            complement(12226..12588)
                     /gene="ipgE"
                     /locus_tag="OO468_002107"
     CDS             complement(12226..12588)
                     /gene="ipgE"
                     /locus_tag="OO468_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR6259220.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            complement(12598..14214)
                     /locus_tag="OO468_002108"
     CDS             complement(12598..14214)
                     /locus_tag="OO468_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR6259221.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            14528..16012
                     /gene="icsB"
                     /locus_tag="OO468_002109"
     CDS             14528..16012
                     /gene="icsB"
                     /locus_tag="OO468_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR6259222.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            16025..16414
                     /gene="ipgA"
                     /locus_tag="OO468_002110"
     CDS             16025..16414
                     /gene="ipgA"
                     /locus_tag="OO468_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR6259223.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            16430..17056
                     /gene="ipgB1"
                     /locus_tag="OO468_002111"
     CDS             16430..17056
                     /gene="ipgB1"
                     /locus_tag="OO468_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR6259224.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            17113..17580
                     /gene="ipgC"
                     /locus_tag="OO468_002112"
     CDS             17113..17580
                     /gene="ipgC"
                     /locus_tag="OO468_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR6259225.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            17586..19328
                     /gene="ipaB"
                     /locus_tag="OO468_002113"
     CDS             17586..19328
                     /gene="ipaB"
                     /locus_tag="OO468_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR6259226.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            19348..20439
                     /gene="ipaC"
                     /locus_tag="OO468_002114"
     CDS             19348..20439
                     /gene="ipaC"
                     /locus_tag="OO468_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR6259227.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            20490..21488
                     /gene="ipaD"
                     /locus_tag="OO468_002115"
     CDS             20490..21488
                     /gene="ipaD"
                     /locus_tag="OO468_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR6259228.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            21497..23398
                     /gene="ipaA"
                     /locus_tag="OO468_002116"
     CDS             21497..23398
                     /gene="ipaA"
                     /locus_tag="OO468_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR6259229.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            23402..23638
                     /locus_tag="OO468_002117"
     CDS             23402..23638
                     /locus_tag="OO468_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR6259230.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            23855..24784
                     /gene="virB"
                     /locus_tag="OO468_002118"
     CDS             23855..24784
                     /gene="virB"
                     /locus_tag="OO468_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR6259231.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(25731..26510)
                     /gene="ipaJ"
                     /locus_tag="OO468_002119"
     CDS             complement(25731..26510)
                     /gene="ipaJ"
                     /locus_tag="OO468_002119"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR6259232.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(27110..27247)
                     /locus_tag="OO468_002120"
     CDS             complement(27110..27247)
                     /locus_tag="OO468_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259233.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
BASE COUNT         9446 a         5657 c         4043 g         8629 t
ORIGIN      
        1 tgtgtgcttc aattaattta acagaccgtt gatatttatc tttgattttt aaaaccttat
       61 ctaattgttt caccaataag ctccattgtc tgctcataag taaaaccgag tcgataatct
      121 tgcttcagaa aactctcaac aacagatatt ttgttgtaga ttttatcctg agaggcattc
      181 tcccccggtt tgtattcacc aaagtcaata attgtcctta gctcttctat ttcagaaagt
      241 agctcccgaa atgcagcggc cataatacga tgtttttcat caacaacctg tgtaaatacc
      301 ctgcttatac tttttaagga atcaattgca ggaaattgtc ctttttgggc tagatttctg
      361 ctcaaatata tatgtccatc taaaatggat cttacctctt cagctaatgg atcagcaaaa
      421 tcatcatcct ccaaaagaac agtgtaaaat gcagtaatag agccacctgc ctttaacttt
      481 cctggccttt caagaagtct gggtaagcta tcaaaaaccg aaaccggata gcctcttctg
      541 gcaggtgatt ctccagcggc taaggccaca tcacgtaatg ctctggcata ccttgttaat
      601 gaatcaataa aaagcgctac tttatgtcct tcagtcctaa aaaattcggc tatagcagtg
      661 gctatatatg cagcattaca acgatcaacc gaagagtaat ccgaagttgc atatactaaa
      721 acacacctgc tttttttctc agagtttttc aaataatcaa ccgtttcagt aacctctcga
      781 cctcgctcac caattaaccc aataacatat atatcagcac cactatgttc aatgagcata
      841 ttcatgagaa aagttttgcc acaaccagct gacgcaaaaa tccccattcg ctgtccttca
      901 ccacacgtga gtaaagaatc aataacctta atacctgtta aaaaaggctt ctcaattgca
      961 gccctttcac tatatagcgg aggagcatta tctacaggtc gataaagaat ttcactgtta
     1021 tctgtaacag caaacttatc agtaacctcc cctaaaggat tgactactgc tcccaataat
     1081 ccacggccga cttgcgtatg aagaaactgg gcagtgggct taatcaacgt ttgccgtgaa
     1141 agtccacgag aatttccaat caagcttaat attgtttttt catcatgaaa tcctacaacc
     1201 tgagctcttg caacaatttc attactttca attccagcct gaatgttaca aatctcacca
     1261 atcgaaacat cgctaagact tgtttccaag attggccccg agattctatt aggaaaagat
     1321 aattgagtga gcaattttgt atagctcatt gattataaga ccccatttaa gatttccatc
     1381 ctctgataaa actcatgcag aatctctgca aaaacaatcc catcgtgaac gtagtcatct
     1441 ttaataacaa ctcttaactg taagtactca tcagaacgat gaagctctac cagctcatta
     1501 atgctataac taaagttcat taacattaaa tttaatatgt tataagcaga tgactgaagt
     1561 tttacatcac ttggtgcatc aaaattagcc cataacataa cctgctcatt gacaagagct
     1621 atattaatag caggcatact atccaaagaa atggtaatcg ctgaatggct gtctaaatct
     1681 gtaataattg atggaggaca accaatcgtg aaaagactat ctctaactaa ttgaactaaa
     1741 ttaatgttac tcatatttaa acctcactaa atagtcttta atacattaat ggtatatgct
     1801 tcatcaatct cagcatacga tataacgagt atactcttaa atctgttatc tataagtctt
     1861 ttaacaaacc tacgtatatc tactgatacc aaaagaacga aatttttttt tgcatttctc
     1921 aattctctca aagcatgtgc taaagtttcc attacctcat ccgaaacctc tatatccata
     1981 ttcaagaaag agccaccaga ggtttgcctt atcccttttc ttattgcatc ctcaatatat
     2041 ccggaaagca tcacaacttt aatctcacca gaaacagcta ttttactaca aatataccta
     2101 gaaagtgatg cacggacatg ttcaactaat gttatgacat ctttttctct gggagcccaa
     2161 agcgccaaag actccataat aagttttaaa ttgcgaacag aaatattttc tccaagcaat
     2221 ctttgtaata cctcagaaat tctctgtata gtcacatgtc ggaagacttc ctttaataga
     2281 tcaggatacc gattttcaaa ctgatctaac atattttttg tttcttgtat accaaaaatc
     2341 tcatttatgt tgtttaataa agcttgtgac aactggtgat aaaattcatc ttgagcactt
     2401 ttaattttag catcaatatt agttagattt tctgtatatg aaacgtctac ccaagaaata
     2461 acacgttcat tatatgaagt tgaaacaaca ggaataccta gagcatctat atctccattt
     2521 tcatctgtaa tacacacttt gtcaaaatat atattaaaac tatcggcacg cacctcattt
     2581 attaacaaaa caatatcatc gaccttaagc tcattgcttg ttctatataa aatagttgga
     2641 agcctgacac cataatcaat aaagaactga cttcttatcc tttcaataag accttccata
     2701 tcatttgcat ttatcttatt ttcggcaaat aataaaatta aaggaacggt ttctgaacta
     2761 atagcatcca gattttctat cattgccaaa gatgagtcat ggctattatc aatatcaaat
     2821 gtaccagtat agccactaga atcagactca gacagacttt tttctttttc tacgaccttt
     2881 ttataataga ataaagccgt caaagtaact gctatcagga aaaaaacaaa aaacggaaaa
     2941 cctggcaaca tccctatcgc caaggcaaga gctgatgtaa caatgagaac aaaaggattc
     3001 ccaaatatct gggacattat gttacgtccc atattatcgc tatcaccatt aactcttgta
     3061 acgataaatc ctgcacttat agaaattaac agagcaggaa tctgagagac caatccgtca
     3121 ccaatagtta gtatggtata agtagataat gcaccagata gggacattcc atgttgactc
     3181 ataccaacag aaatacctcc tattaaattg acaaaaataa taatgatgcc agcaattgca
     3241 tcacctttaa tgaacttcat tgctccatca aatgaaccat acaattgact ctcacgttcc
     3301 agaatactgc gtctttcttt agctcctgcg gcatcaataa ttccagcctt taaatccgca
     3361 tcaatgctca tttgtttccc cggcataccg tcaagagaaa agcgagcagc tacctccgca
     3421 accctttcag atccttttgt tataacaatg aactgcacaa cagttactat tgaaaaaata
     3481 acaaaaccta cagccagact atcaccaatg acaaactggc caaatgtagt aataatttta
     3541 cccgcatccg catcaactaa aattagtcga ctagtactta ttgataaagc gagacgaaat
     3601 aatgtcgtta tcaacaaaac cgaagggaat gtagaaaaac tcaatattct ttcaatataa
     3661 aatgatccca taaaaacgag aatagcaagt acaatattaa gccctattaa aaaatcaaca
     3721 aggtatgtag gtaatggtat gatcaacata gcaatgatca taaccataag cactaatata
     3781 attaattcag gcttagtact tacttgctta agaaaagact ggatcacttt tatctcctgt
     3841 tatctagaaa gctctttctt gtatgcactt gtgacaagct ctcgtagaat attgatcgca
     3901 atttctcttt cactttcact tttaaatata tcatgaggac tttcattaac agaagataga
     3961 tatgtttgat acactacaga atcctcatga ctggctcgtg atacttctat aacagaagta
     4021 agaatttgct cactttcact tggatattga aaaatatata ataaagttag caatagctgc
     4081 tctgcactta aactgccatc ttttagcaag cttgaggatt ccagtttctt cataaaaatt
     4141 acgtctgaag ttctaatcat cttaattgca attaatttag ataacacaaa accaaactca
     4201 taagcattac agctcggttt ctcagaatcc atgtctacaa tcagtgactg ctctacaaaa
     4261 ttcacaattg tatgtctgtg attaaaacca aaattaccaa gccattctat atactgatct
     4321 gttgttgata tgttccccat gataaaacca cgataacatg cacgcaaaag ctgtggttca
     4381 agtttcattt tgttgccaaa taatttagcc tgtatagctg aatttattcc agcattaaca
     4441 cttttttcac tacctgatat tatctcatta ataattttag ttaataattc gacttgttca
     4501 gcagtaagat ctttttcatt caaaagttcc cttaatgcta agacttgatc acttggatct
     4561 ttaaaatatc tctttagtct gtctataaaa tcccgatcta gaggaaggtt atcttttaac
     4621 gttctcttta aatcaaaaat cttgtgatta atttcatctt cttccccatc taaaatacgt
     4681 tcctgactat cactatttgt tcctttcagt ttctcaaggt ctcttctgtt tataaatgac
     4741 gaaagagcgg aagccatctc atcagatgag tcaatatact tgctattagc caagccagaa
     4801 tcaagctctg catcagcagt ctcatctcca tcatctgaat ttgtcattgc attcaactta
     4861 tcaatgaatg cagagctaaa aactcctgta tttttaacat caagcataca tcacctactt
     4921 tatagttagt aatttaagta tgaaaccaat gatttttcgt cttccagcaa agtagtttca
     4981 gatgggatta tttgtgtagt cttttgtatt tctctttcag atatgaggct tttatattct
     5041 gcagtattat aataacttga ctcctttatc tcacggggtt gaatcagaaa aacccttaca
     5101 atattactga tattacttgt tttatacttg aacacattac cgattacagg aatagaggag
     5161 agaaaaggaa tagatattat ttcgttagag ttcgtttcat gcgtataacc acctatcaac
     5221 aaacttttgc cttgtggaac tctcgctatt gtactgattt tggtccttcc aacttctgga
     5281 aggacagatg tattttcatt attataattg taatttgact gactatttcc tgttccatcc
     5341 tctatcgtta aagacatctc aatttgtccc cgagatgaaa agcgaggtat aacatttatc
     5401 aatgttccat acgtaacatg ctccaacgaa gaattgcgct caccaactaa tgacacataa
     5461 aaagtcctgt tattatcaaa tatagccgga atattctctt gtgtcaaaat caccggcctt
     5521 gaaactacat ttgctttctt tttctgattt aatgccataa ctgaagcaat aaacttattg
     5581 ccgtccaaag tgcttatcga tgctgatgag ctcatattaa atgacgcacc aaacgaatca
     5641 ccaaatgaag ccgttccttg ccaattaaca ccaaggttat ttaattcaga tttatcaata
     5701 tcgattatcc ataatgataa ttctatatgt cgtttggcta tatctaactg agtgataata
     5761 tcgcgaataa tctgtatttg ctgatcatta cctttgacta atatagaatt ggtttctgga
     5821 tatgcaataa gtgatacatc ctctaatata gaactattgg taactgaact aaatgaaaag
     5881 tcattactat cctcagatac tttctgtgta atattaaagg gtggcatcgg atcattttgg
     5941 gcttgtctgt tactcagagc cttaccatta tttaataaac gttctacaac agtagccacc
     6001 cctgggatga caatatcctc ccctctcata ttataagttc tgtcactaac aaatgtattt
     6061 tttaatttta taaccccaaa attaacttta tctgtcccaa tagaagaaac ctgcttatca
     6121 agcaaagttg ctgtattagc taccagttca accaatgccg gaggaccaga tatatagaat
     6181 gttttatcac taatgtttcc tcttatagga tatctgtgat cataaaggtt agcatccttc
     6241 agatactgaa taagataatt aagtgatata ttctccagca aaataacttt gcttattaat
     6301 tctccagagt catatatgta taatgcattt ccatctttat accatatgag tcccacaagt
     6361 aatgtcagtt tttcaagcat ttcttctgga ttagataaat caaactcccc agatattctc
     6421 ttttttgcag cttgtttgct aacaacaata ggataattta ataacgcaga aaaacgttca
     6481 aaaaaagatc ctaccgtatc actttgagca acatatttcg ctatgtcatt ttgttctaat
     6541 aaatgagagt caatattatt agcattaact atcaatggta acaatacaat caagagagtc
     6601 aaagatttaa tattaaattt tttcatttat ttttttcact aaaaaagtaa tattcgataa
     6661 ttctcttgca gaaaagccta gtcgtgtttt tatctcattt gaaaaatggg atgttgatgc
     6721 atacccatta ttcatggcag ctgaagtgat tgtttgattg tgcaaaaata catctaataa
     6781 accatttact aaccgccaag tgttcagttg ttctttgact tttgccccta aagcttttct
     6841 acataaagat ctaaaataag cctccgacac accataatgc tctgtcagac tctttattct
     6901 tacctccttt tctttctcaa tagttctcag aagataaaga accaaatgat atgactcagt
     6961 cattcgtatc atagccagtg cattacttac attctggtca gatgaaaaga tgaatcttaa
     7021 cattaactta tcaagcatcg attcggtatt acaactatcc cgaagatcat tctcatgata
     7081 gaaatgaata catttttcag catggctatg ctctactaga gcaccactga aatagtccca
     7141 aaaagcaaga aattttgcac aactattaat gttaaagcga taatgatagt tcacattatc
     7201 actgaatttt atattttgat ttcggacaaa aaaaaaccct tccatgctgg agccagctca
     7261 attatgttac gttctttatc ttttacaagc tcaacgttaa ctggttcatg tccactagag
     7321 taaatgtaaa acatattact tgtatttaga cctttatatt tactcacaat acaattatat
     7381 ccctcgaatt aattttaatt taacttcccc atccttctct ataaggatgg tatcatttat
     7441 cttattacct tttaaaacaa ccccgtcttt cagcatagaa aatagtttat ctatagctat
     7501 cttttgtgca tccatgaagc acccatctac ttccttaaca ttaaagttgc ccatgaattt
     7561 acctgcatta aaactataaa ccgccttacc actaatacat ttagagtaaa catttatact
     7621 tttattggtt gtattaaacg accataataa atcatttgga atagaaaaat aatccttact
     7681 tacaggaaca atatgccatt ctttttcaga attagatgat gactttaaag cacacccact
     7741 cagtgttaat agcaatatac ttaaaataaa tattttcaac ttattactac catgtcgaat
     7801 catctgcctc tctccctgct tcaaagacac taattaatga atcgaaggtt gtagtcttaa
     7861 tatagaaatc aatattgtta tatatggaca atgatgcata aaatccctca tgcaaatcga
     7921 catattgatt tgcatctatg agtttattaa gaagtgaact tttttgtgca gctgtaagat
     7981 aaggaaaacg tgaattgaat aattgttcaa tcttattgtg cagttcatca ttatctatgg
     8041 aatttaattc actctgtgag aaatattgca ttatttcata gattatatcc tcatcaaatg
     8101 attgcgaaga tgataatctc tcatttaaat taacaacttc ttcactattt aatctttgct
     8161 gcagagcatt gcttgcattt atttgattaa tcattaacag gattctcttt taacaagtct
     8221 tctgcaattt gtctcagttt cttatgtgct aactctttga aatatttttt atcatttttt
     8281 gttaattttt ccgcaagttc tattttaaca cctgatatta catcctgagg actaaactcc
     8341 actacttgat taccactaca gaatatataa ttattccctt cagaatattt taaaataatc
     8401 ttgacatctg agcgatattt atggagatca agttctaact ttttcctcag tgctaaatta
     8461 cacttgggca aaaccacttt taattctgtt actgtattac gcaacgaact aagccactgc
     8521 tccaataaag tgctaatgat cctctcatca tgtaaatttt ctgaaagcaa actagttata
     8581 tagtcctcaa tatttcttct attttcattt tgctttaact tccattcaca aatatagtca
     8641 ataatatgct caaaagcagt ctgtatacca atttgataac catcacatac agcatctata
     8701 cgcagtgatt cagctttttt cgtagcctcc tttataacac aaattgcttt cttcttactc
     8761 tcagtgtctc tatcttttat ggttttacat aattcctttt caattctctt tataacaaca
     8821 ccatcatagg catgatgtct ggataccggt aaagtgcctt tttgcatatt gcataccttc
     8881 atttaatcgg gacaaatttc tttctacagg cgtataattt ccctccaaag caattggatt
     8941 aaataataag tttattcttt catatatacc aacagatata ttttccttcc acgcaataag
     9001 atgccaaaaa ccacttcgaa tagcggcctc cccttgctta ctgattttgc ccccataata
     9061 actaataacc atctcactat gattaattaa ccgagactct tgactaccga taaaataagc
     9121 aacagctgga agtaaattcc agttggcaac aaacatagcg gttaccccgc ttaaaggttc
     9181 aatatcgcaa tttagatcat atttattaat tatgagatta ttaatctcac ttctgattac
     9241 gccattttct aacagttcag aaggcaaatt caaccgattt ctatttatat agaacgctgg
     9301 atcaaaaatt attgaaagat attttttata aattccatcc attcttatca tattttgttt
     9361 ctcttgaacc accctgtttt gaatgcccat accaaaagaa tgacaactag tacagccatc
     9421 ccgagaaata aatatattac ttcatttgtt aaaaattccg atttaacttc cttaacaggt
     9481 tgtacatttg tataaacata ttcttctttc ggagttaata tgacagatat attttcatac
     9541 ttaacatcag aaaaggtgtt tttcaaaaat cgcttaatat tactaactaa tagttcagac
     9601 tctttcggtg agtcatatat agcgattact gatatatgca tcggttttga agatatattt
     9661 ttttcttcaa gatcatagct aacatgtatt tttgccgaaa taacaccacc aatagaaact
     9721 aaagactgtt ccagccgttg ctcaatagca ctatataaac gggccttttc agctcttgga
     9781 gaagacacta atgaatctgt aggaaacatt tgtgagatat caactctctc cggatttggc
     9841 aaatcgtaca tgcgcatcaa atcaactgcc gatgcaaatg tccccttttc gacttgtacc
     9901 gagatcccct gtttacctcc atcaactttt ctagcagtaa tattatggcg ttctagcaca
     9961 gatattattt catttgcctg tctttgagat aaattagaaa ttaactcttc acgttgctca
    10021 catccaatca acatcagcaa caagaataaa ataaaacctt tatacctaat catataaatt
    10081 aagactttaa taaagtttca acagccgaaa ctgtttttct tgctaacgtg ccagctaatg
    10141 atactcctat tgaataattt gagagcgtcg tctgtaactt ggccaaagat tctgggttta
    10201 atgattctgg attgcttacc atctccatta tttgtgatac ttgaatatct gtatccatct
    10261 taatatcact atatttagcc gacacgacgt cttccagact tgatatctct tgagattgaa
    10321 aatcactggc tttgataata tcaacctgat tgactggata aatgtaattc atgaatgctc
    10381 cctattatct gaagttttga ataattgcag catcaacatc cttaatcact ttcactgtat
    10441 tggattgcgc gttcctatat aatgtatatt cagataattt actttggtat tcagccagca
    10501 actgtggatt cgaaggattt ttagctaatt tatctagtgc caatgttagt tcaccttgta
    10561 atgtttgagt tccatcatca aaagtttcag ataatgaact caatgtccaa tcatcattcg
    10621 gtactgtaac actcatttta tcctcactta tttttatcta aaaaaaacca gtgtttatca
    10681 ttcaacatta cataactgtc tttgctgtta agatatgatt tacctttaaa atcatcatct
    10741 atcaaaagta cagaaaactc aatatactga tctccatata tatttttatg ttcagaaatc
    10801 agtctattaa tgagctcaag cttttcatct gtcagttctt ctctgacaga atatgtaact
    10861 ttattatttt cacaaatctc tttatactgg acatttacct ttgtgaaaat acctttagca
    10921 tccccccttg cattatgatc gctaagatag ttgaatttaa tatttttgat atacggaaat
    10981 tcaacaatca gagcattttt caatttatca agttcggtat cttttgaact atttctttct
    11041 ttacttaaaa acaccagtaa ttccgcttta tcactcacta aattcaacac atacaaattg
    11101 ataaatggga atcgttgatt gatatattgt tgaattctat ttatttcttt gtttgacacc
    11161 agaatatatc tgcatttggt aaatccggtt ttatttaagg ctacagacgc ccataccgca
    11221 tcattttgtg tttcggcaag aatataattg cactgtccag tgttgactat tccatatctc
    11281 ttatcatcta taatttcagc aatttctttt acttcatctt tctttaaaga gagggaaaaa
    11341 attataatta acacaacaat aacagcaaag aaaaaaaaca ccgagtggtt cttatacatt
    11401 ccgtttagta tatggcctct cgatttatcc tccctcatat ttttaaggtg aaaagaaatc
    11461 ccgtcatatt caaatgaatt aaattgtgaa ttgatatcaa tttgctcacc tttttcatca
    11521 ctaaattgac agatattatc tttgcttatg gtcaaataaa ttataccatc cgatttacta
    11581 tctgttacag gaataatatt ttctgggaaa ttgtcgtttc caaactcaag tgtttctatc
    11641 gtccgtccga gaacaattaa gttattccca taatataacg gaaactcatc cccgacaccg
    11701 tttgtgagtt tcactaataa cctgaatggt gcaagatttg agttctttgc ctcagacata
    11761 aatcctctta tatccttcga ttattctgct tgctctctgc tgctatccgc aaatagcgtc
    11821 tatagatatc ctctgcgtac tttaatcttt cttttttctt tttcggagac gttcctgcat
    11881 tataggctcc aacagcctcc caccctcttc catacatcat cataaactca tttaataact
    11941 ttgctgcata atgtacagaa agacatggat gactaattag catttcctca gaatatccca
    12001 tttctctaag tcttttagaa tgaaagtcat ttatttgcat tataccataa tctttacttc
    12061 cattgttatt aacattgact gcagatttat taaatccgga ttctttttcc gcaatcgctt
    12121 ttaacaaact tgatggaata ttgtaccttt caccagcctt atcccaacaa tcagctctcc
    12181 ctaaatatgg gataaaacac agcaaaataa atacgaaacg ggacattaat accccttcat
    12241 tcttcgcgca aattcatcct tcagccaacg cacacgcgaa atataatatt ctgtattaac
    12301 atatacatcc tcgaattcat ttatcccggt aagatctaat cgcgcaatta gatttccgcc
    12361 ttcatcatca gtagctaagc atatcttttc tgagtaattt aggctcaacg catatataag
    12421 atcatttata ttttcaggca atgcacaaaa agggcataat aaatttattg aatctccttc
    12481 tttttcaata tatattagca catcatcatc aagcatgatt gcttgatcat cgatatcaat
    12541 taaagggata cccaaggctc ggcaaataac atctgctaaa tcttccatat attcctctta
    12601 tacaaatgac gaataccctt tcaccatatt ccatattttt gggtccccta ttctttcaga
    12661 ataagatagc tctaacgaag atagcggcaa ttttttcatg actttatttc cgggcacacc
    12721 agtattcatc tcttggattt ccatattacc actattcatt agaatagtag aaaataatct
    12781 tttctcttct gaggataatt tactatttaa ttgagaaaac tgacctgttt catgttttct
    12841 tattatttct cttttaattt cagcatcctg catacctgtt ctgtccttcc cactcttaca
    12901 attccagcaa ggtacagcac ctatagtata agccaataga gtcattcttt gtgacaattt
    12961 atacggctct ccattatcat ttttttgtaa ctttttggtt acaatctctt ttatctggtt
    13021 agccagatat atcacatcat tcttacatgg tggatttttt tctattgctt ctgcagccca
    13081 acctccaatc acaccatttt tgaggaaatt atcccccaac aaagaacata ttgattcatc
    13141 attaagcttg tcaacattcc tccagcctaa gcccattttt agcgctaatt catttacacc
    13201 aaagttaaat gtcaccactt ttagattaac gcttacttct ttcagaagac catcactatt
    13261 ccgaattaat aatttcgttg gcttccccct tttagagttt aaccctttta atgcgttcac
    13321 ctgatctttt aacatactct cctctccccc ggttaaactg gtcggcgtca gaagagaagt
    13381 cgaaacaatc tttaaatcta ctgttttacc agacaaagcc tgtgataata actctggtct
    13441 gctatataat gctgcgctta ctaactcctc agctttatta cgagcagcaa cagctctttc
    13501 agatgagttt tttttcaatc cataggctga tataactcca tgtctgatac ccgaaaaaat
    13561 ttccttcccc tcatcatcca ccacttttga aagccacata ttcgcgatat gatcactctc
    13621 tctcgtagaa gcacaacaaa ttccttttcc attatactct ttgacaaata tatttttatt
    13681 ccctatcttc atatgagagg caggagttaa ttgaaaacca taatttttcc catgatgact
    13741 aatattcttg tcgaccggtc cccaattctt attatttaaa taatcagtaa atatttgttt
    13801 tattttcgtt tgcaattcct ttttattttt aatgcctagc tgtgctctta tctcttcact
    13861 tattacttta ttaagctcat taatatgtct tttattaact tcaccaagtt tataggtatc
    13921 taatcgttcc atcatggctt taatttttga cgtatcacca cctgccaact ttattgcctc
    13981 gcaaaaaact tcagctctgt cacgcgcaaa caaaaggctt tttcctgtta agcttgtttg
    14041 gttctgtaaa tataatcgat taagcgcttc gatcccctta tttctttctc catttttaac
    14101 tctttgatat gaaatcacgc ttacaccttt gtgctttccg gtttcttctg cgcctttata
    14161 ggaatctccg ctttgaaatg aaacctgatg caatcccaaa ttagttatgt gcatttaatt
    14221 atcctcagtc ataaatgggg cattgattgc agttttgtac ctcgttttac aacaaaaaag
    14281 atacttattc aacagggttc tctctttttc tataacactc cgagcctgat acatcaggca
    14341 ctttatcttg tgggatttca tgatgaaacg agcactacat atgctcacga ggtacatatt
    14401 atgtacagca aaagaaaatt tgattatgtt aacaggctca agtttctaaa tttattaatt
    14461 gaatatatta tataccaaat acattttaca tccccacaat caccaagtaa tggagagtta
    14521 ataaagtatg atcctcaaaa ttagcaattt cattgacgca agcaatacaa aagggcctat
    14581 acgcgttgaa gatacagagc atggacctat attgatagca cagaaattca acctaaagga
    14641 tctctttttc agaacattaa gcactattaa tgcaaagata aattcacaga tattaaatga
    14701 acaactaaaa aattatcgac tagagaatca aaaatcctta ttgcttttcc tcaatacatt
    14761 agctagcgaa aaatctgcag aaagtgcgtt tgccgcctat gaagcagcta aaaactcaat
    14821 tcaacactct ttcacaggta gagacatcaa attaatgttg aataccgcag agcgttttca
    14881 tggcatcggt acagccaaaa atcttgaaag acatttagtt tttcgttgct ggggaaatag
    14941 aggcataacc catttagggc atactagtat cagtataaaa aacaatttac tacaggaacc
    15001 aactcatacc tatctcagct ggtatcccgg cgggaatgtt acaaaagata cagaaataaa
    15061 ctaccttttt gaaaaacgct caggttacag tgtcgatacc tataagcaag acaaactaaa
    15121 tatgatttca gaccaaacag ccgaaagact tgatgcaggc caagaggtta gaaacttatt
    15181 aaattcaaaa caagatcaaa ataacaacaa aaaaatattt tttcccagag ctaatcaaaa
    15241 aaaagacccc tatggttatt ggggggtcag tgctgataaa gtatacattc cattatcagg
    15301 tgataataaa acaaaggatg gtaaaatcag ccataacctt tttggccttg atgaaacaaa
    15361 catgtcaaaa tttatatgca aaaagaaagc agatgctttc agacaattgg caaattataa
    15421 attaatcagt aaatctgaaa actgtgctgg tatggcactt aatgttctta aagcaggaaa
    15481 ttctgaaata tactttccac tccctgacgt taaattggtt gctacaccta acgatgtata
    15541 cgcgtatgca aataaagtta ggcaacgcat tgaaagtctc aatcagtctt ataacgaaat
    15601 aatgaagtat attgaatctg attttgatct ttccagattg actcaattac gacgcagcta
    15661 tcttaaaagt ttcaataaaa ttaaccttat ccacacacca aaaacattca agcctttatc
    15721 aatttcactt tacaaacatc ctactgaaaa tgtatcttca gaagactttg atgcggtcat
    15781 caacgcttgc cactcatact tagtaaaatc agcacccagc aatatgactc gtgtattaaa
    15841 cgaattaaaa accgaagcaa cagataaaaa agaagaaatc atcgaaaaat caataaaaat
    15901 tattgattat tacaattctc ttaaatcgcc agatcttgga accaaacttt acattcacga
    15961 cttattacag atcaataagc ttttattgaa taactctcat tctaatatat agaaggccat
    16021 agaaatgtgt cgcaaactat atgataaact ttatgaaata acaggtgcaa agcttgattt
    16081 taacgataaa aatcaagcct ttatattgct cgaagaacaa atacctgttt gtataacaga
    16141 taatgatgaa tacatctttt tgaccggact tcttaatgaa catgaacttt ttacagaaaa
    16201 tattattaat ccagaacaca tactcatcct taactactct ttgagtagag attatggttc
    16261 ttctatctgt cttctccctg atactcatca atgtgttttg accaagaaac actacaaaaa
    16321 atatctttca cctgatgaac tcattgaatc tttgtatgaa tttttatttt gtattaaact
    16381 aaccattgca aacatcactt cagaagtgaa ctaacatata gggggtatca tgcaaattct
    16441 aaacaaaata cttccacagg ttgaattcgc gattcctcgc ccatcatttg attcgttaag
    16501 tcgcaataag cttgtaaaga aaatattatc tgtttttaac ttaaaacaac gctttcctca
    16561 gaagaatttt ggatgcccgg tgaatataaa taaaatcagg gacagcgtca tagacaaaat
    16621 aaaagatagt aattcgggga atcaactatt ttgttggatg agccaagagc gaaccactta
    16681 tgtctcttca atgataaaca gaagtataga tgaaatggcc atacacaatg gagttgtttt
    16741 gacctcagat aacaaaaaaa acatatttgc agctattgaa aaaaaattcc cggatataaa
    16801 acttgatgaa aaatcagcac aaacatctat cagtcataca gcactaaacg agattgcctc
    16861 atctggcctc agagcaaaga ttttaaaacg ctatagcagt gacatggatt tgtttaacac
    16921 acaaatgaag gatcttacaa atctagtatc cagttccgtc tacgataaaa tatttaatga
    16981 atcaacaaaa gttttacaaa tagaaataag cgcagaggtt ctaaaggctg tataccgtca
    17041 aagcaataca aattaaatct tttatattaa atcttatact tattaaagta aagcagacaa
    17101 aaaggagacc ttatgtcttt aaatatcacc gaaaatgaaa gcatctctac tgcagtaatt
    17161 gatgcaatta actctggcgc tacactgaaa gatattaatg caattcctga tgatatgatg
    17221 gatgacattt attcatatgc ttatgacttt tacaacaaag gaagaataga ggaagctgaa
    17281 gttttcttca ggtttttatg tatatacgac ttttacaatg tagactacat tatgggactc
    17341 gcagctattt atcagataaa agaacagttc caacaagcag cagaccttta tgctgtcgct
    17401 tttgcattag gaaaaaatga ctatacacca gtattccata ctggacaatg ccagcttcgg
    17461 ttgaaagccc ccttaaaagc taaagagtgc ttcgaactcg taattcaaca cagcaatgat
    17521 gaaaaattaa aaataaaagc acaatcatac ttggacgcaa ttcaggatat caaggagtaa
    17581 ttattatgca taatgtaagc accacaacca ctggttttcc tcttgccaaa atattggctt
    17641 ccactgagct tggagacaat actatccaag ctgcaaatga tgcagctaac aaattatttt
    17701 ctcttacaat tgctgatctt actgctaacc aaaatattaa tacaactaat gcacactcaa
    17761 cttcaaatat attaatccct gaacttaaag caccaaagtc attaaatgca agttcccaac
    17821 taacgctttt aattggaaac cttattcaaa tactcggtga aaaatcttta actgcattaa
    17881 caaataaaat tactgcttgg aagtcccagc aacaggcaag acagcaaaaa aacctagaat
    17941 tctccgataa aattaacact cttctatctg aaactgaagg actaaccaga gactatgaaa
    18001 aacaaattaa taaactaaaa aacgcagatt ctaaaataaa agacctagaa aataaaatta
    18061 accaaattca aacaagatta tccgaactcg acccagagtc accagaaaag aaaaaattaa
    18121 gccgggaaga aatacaactc actatcaaaa aagacgcagc agttaaagac aggacattga
    18181 ttgagcagaa aaccctgtca attcatagca aacttacaga taaatcaatg caactcgaaa
    18241 aagaaataga ctctttttct gcattttcaa acacagcatc tgctgaacag ctatcaaccc
    18301 agcagaaatc attaaccgga cttgccagtg ttactcaatt gatggcaacc tttattcaac
    18361 tagttggaaa aaataatgaa gaatctttaa aaaatgatct ggctctattc cagtctctcc
    18421 aagaatcaag aaaaactgaa atggagagaa aatctgatga gtatgctgct gaagtacgta
    18481 aagcagaaga actcaacaga gtaatgggtt gtgttgggaa aatacttggg gcacttttaa
    18541 ctatcgttag tgttgttgca gcagcttttt ctggaggagc ctctctagca ctggcagctg
    18601 ttggtttagc tcttatggtt acggatgcta tagtacaagc agcgaccggc aattccttca
    18661 tggaacaagc cctgaatccg atcatgaaag cagtcattga acccttaatc aaactccttt
    18721 cagatgcatt tacaaaaatg ctcgaaggct tgggcgtcga ctcgaaaaaa gccaaaatga
    18781 ttggctctat tctgggggca atcgcaggcg ctcttgtcct agttgcagca gtcgttctcg
    18841 tagccactgt tggtaaacag gcagcagcaa aacttgcaga aaatattggc aaaataatag
    18901 gtaaaaccct cacagacctt ataccaaagt ttctcaagaa tttttcttct caactggacg
    18961 atttaatcac taatgctgtt gccagattaa ataaatttct tggtgcagcg ggtgatgaag
    19021 taatatccaa acaaattatt tccacccatt taaaccaagc agttttatta ggagaaagtg
    19081 ttaactctgc cacacaagcg ggaggaagtg tcgcttctgc tgttttccag aacagcgcgt
    19141 cgacaaatct agcagacctg acattatcga aatatcaagt tgaacaactg tcaaaatata
    19201 tcagtgaagc aatagaaaaa ttcggccaat tgcaggaagt aattgcagat ctattagcct
    19261 caatgtccaa ctctcaggct aatagaactg atgttgcaaa agcaattttg caacaaacta
    19321 ctgcttgata caaataagga gaatgttatg gaaattcaaa acacaaaacc aacccagatt
    19381 ttatatacag atatatccac aaaacaaact caaagttctt ccgaaacaca aaaatcacaa
    19441 aattatcagc agattgcagc gcatattcca cttaatgtcg gtaaaaatcc cgtattaaca
    19501 accacattaa atgatgatca acttttaaag ttatcagagc aggttcagca tgattcagaa
    19561 atcattgctc gccttactga caaaaagatg aaagatcttt cagagatgag tcacaccctt
    19621 actccagaga acactctgga tatttccagt ctttcttcta atgctgtttc tttaattatt
    19681 agtgtagccg ttctactttc tgctctccgc actgcagaaa ctaaattggg ctctcaattg
    19741 tcattgattg cgttcgatgc tacaaaatca gctgcagaga acattgttcg gcaaggcctg
    19801 gcagccctat catcaagcat tactggagca gtcacacaag taggtataac gggtatcggt
    19861 gccaaaaaaa cgcattcagg gattagcgac caaaaaggag ccttaagaaa gaaccttgcc
    19921 actgctcaat ctcttgaaaa agagcttgca ggttctaaat tagggttaaa taaacaaata
    19981 gatacaaata tcacctcacc acaaactaac tctagcacaa aatttttagg taaaaataaa
    20041 ctggcgccag ataatatatc cctgtcaact gaacataaaa cttctcttag ttctcccgat
    20101 atttctttgc aggataaaat tgacacccag agaagaactt acgagctcaa taccctttct
    20161 gcgcagcaaa aacaaaacat tggccgtgca acaatggaaa catcagccgt tgctggtaat
    20221 atatccacat caggagggcg ttatgcatct gctcttgaag aagaagaaca actaatcagt
    20281 caggccagca gtaaacaagc agaggaagca tcccaagtat ctaaagaagc atcccaagcg
    20341 acaaatcaat taatacaaaa attattgaat ataattgaca gcatcaacca atcaaagaat
    20401 tcgacagcca gtcagattgc tggtaacatt cgagcttaat atatccaaga gccataataa
    20461 tatatggctc ttcctgtaag gaaataacca tgaatataac aactctgact aatagtattt
    20521 ccacctcatc attcagtcca aacaatacca acggttcatc aaccgaaaca gttaattctg
    20581 atataaaaac aacgaccagt tctcatcctg taagttccct tactatgctc aacgacaccc
    20641 ttcataatat cagaacaaca aatcaggcat taaagaaaga gctttcacaa aaaacgttga
    20701 ctaaaacatc gctagaagaa atagcattac attcatctca gattagcatg gatgtaaata
    20761 aatccgctca actattagat attctttcca ggcacgaata tccaattaat aaagacgcaa
    20821 gagaattatt acattcagct ccgaaagaag ccgagcttga tggagatcaa atgatatctc
    20881 atagagaact gtgggctaaa attgcaaact ccatcaatga tattaatgaa cagtatctga
    20941 aagtatatga acatgccgtt agttcatata ctcaaatgta tcaagatttt agcgctgttc
    21001 tttccagtct tgccggctgg atctctcccg gaggtaacga cggaaactcc gtgaaattac
    21061 aagtcaactc gcttaaaaag gcattggaag aactcaagga aaaatataaa gataaaccgc
    21121 tatatccagc aaataatact gttagtcagg aacaagcaaa taaatggctt acagaattag
    21181 gtggaacaat cggcaaggta tctcaaaaaa acgggggata tgttgtcagt ataaacatga
    21241 ccccaataga caatatgtta aaaagcttag ataatctagg tggaaatggc gaggttgtgc
    21301 tagataatgc aaaatatcag gcatggaatg ccggattctc tgccgaagat gaaacaatga
    21361 aaaataatct tcaaacttta gttcaaaaat acagtaatgc caatagtatt tttgataatt
    21421 tagtaaaggt tttgagtagt acaataagct catgtacaga tacagataaa ctttttctcc
    21481 atttctgagg tgcgccatgc ataatgtaaa taatactcaa gcgccaacat tcttatataa
    21541 ggcaacttca ccatcatcaa cagaatacag cgagttaaaa agcaaaatat ccgatatcca
    21601 tagttcgcaa acttctctaa aaacaccagc atcagtgtct gaaaaagaaa actttgcaac
    21661 gtcttttaat cagaaatgtc ttgatttttt attttcttcc tcagggaaag aagatgtgtt
    21721 aagaagcatt tattccaact caatgaatgc gtatgccaaa agcgagattc tcgaattttc
    21781 aaatgttttg tactccttag tacatcaaaa tggtcttaat tttgaaaacg aaaagggact
    21841 tcaaaaaatt gtcgcacagt attcggaact aattataaaa gataaattat cccaagattc
    21901 tgcctttgga ccatggtcgg caaagaataa gaaactccat caattacgac aaaacattga
    21961 gcacagactt gcactattag cacaacaaca cacatctggt gaagctttat cattgggaca
    22021 aaaactcctc aatactgaag tatcatcatt tatcaagaat aatattcttg ctgaattaaa
    22081 gttaagtaat gaaactgttt catctctcaa actagatgat ttagttgacg cacaggcaaa
    22141 acttgccttt gatagtttgc gcaatcaacg taaaaatact attgatagta aaggatttgg
    22201 tataggtaaa ctgtcaagag acttaaatac agtagccgtg tttcctgagc tgttgagaaa
    22261 agtccttaat gatattttag aagatataaa agattcgcat cctatccaag atggcctccc
    22321 tacacctccc gaagatatgc cagatggcgg accaaccccc ggagccaatg agaaaacatc
    22381 ccaacctgta attcactatc atataaataa tgataataga acttacgata atagagtttt
    22441 tgacaacaga gtatatgaca atagctatca cgagaaccca gaaaatgatg cacagtctcc
    22501 tacttctcag acaaacgatc tattatcccg taacggaaac tcattactaa atccacaaag
    22561 agcactagtt caaaaagtaa cttccgttct accacactct atatcagata ctgtccagac
    22621 atttgcaaat aattcagctt tagaaaaggc tttcaaccat actccagata attcggatgg
    22681 aataggttca gacctgttaa ctacgagtag tcaagaaaga tctgcaaata actctctttc
    22741 tcggggacac aggcctctga acatacagaa ctcttcaacc accccccctc tccacccgga
    22801 aggagtgaca agcagtaatg ataactcatc agatacaact aaaagtagcg cttctctttc
    22861 tcatagagta gcttcgcaaa tcaataaatt caactcaaac actgattcaa aagtacttca
    22921 gactgatttt ttatcaagaa atggagacac atatttaaca cgggaaacga tatttgaagc
    22981 ttcaaaaaaa gtaacaaact ccctaagtaa tcttatatct ctcattggaa ctaaatcagg
    23041 aacacaagaa cgagagttac aggaaaaatc aaaggacatt acaaaatcca caacagaaca
    23101 tagaataaac aacaaattaa aagttacaga tgcaaatata agaaactacg taacagaaac
    23161 caacgcagat acaattgata aaaatcatgc gatctatgaa aaggcaaaag aagtatctag
    23221 cgccctcagc aaggtattgt caaaaattga cgatacctct gcagaattac ttacagatga
    23281 tatatctgat ttaaaaaata acaatgatat tacagctgaa aacaataata tatataaagc
    23341 agcaaaagat gtaaccactt ccctatcaaa agtattaaag aatatcaata aggattaaaa
    23401 tatgataaaa gaaaaaatat tatcaatagt ggccttttgc tatggtatag cgtacagcaa
    23461 actatctgaa gaaactaaat ttattgaaga tctttctgcc gattctcttt ctctgattga
    23521 aatgctggat atgattagtt ttgagttcaa tctacgtata gatgaatcta cattagaaca
    23581 cattattact attggagatc ttatttctgt agtcaaaaat agtacaaaat caatatagta
    23641 attgataagc attttttcat ctatggagct ctcacatcag agctccacaa gaatattatt
    23701 cttttatcca ataaagataa attgcatcaa tccagctatt aaaatagtaa tttttaagac
    23761 taccgttgac tatcatcaac agttacatac aatcttgctc acactgcatt taacttttgt
    23821 caatataaat caatatgaat aaacagggtg tgatatggtg gatttgtgca acgacttgtt
    23881 aagtataaag gaaggccaaa agaaagagtt tacactccat tctggtaata aagtttcctt
    23941 tatcaaagcc aagattcctc ataaaaggat ccaagattta accttcgtca accaaaaaac
    24001 gaatgtacgc gatcaagaat ccctaacaga agaatcatta gccgatatca taaaaactat
    24061 aaagctacaa caattcttcc ctgtaatagg aagggagatt gatggtagaa ttgaaattct
    24121 ggatggcact cgtagaagag catctgcaat atatgcagga gcagatcttg aagttctata
    24181 ttcaaaagaa tatatatcta ctcttgatgc cagaaaacta gcaaacgata tacaaacagc
    24241 aaaagagcat agcatccgag aacttggtat tggtcttaat tttctgaaag tatcagggat
    24301 gtcctataaa gacatagcca aaaaagagaa tctgtctcgc gcgaaagtca ctcgtgcctt
    24361 tcaggcagca agcgttccac aggaaataat atctctattt ccaatcgcgt cagaacttaa
    24421 ctttaatgac tacaagatat tattcaatta ttataaagga cttgaaaagg ctaatgaatc
    24481 tcttagttct acactaccaa tattaaagga agaaataaaa gaccttgata caaatttgcc
    24541 cccggacata tataaaaaag aaattttaaa catcataaag aaaagcaaaa acagaaagca
    24601 aaacccttcg ctaaaagttg actcgttatt tatttctaaa gacaaacgga cttacataaa
    24661 aagaaaagag aataaaacaa acagaactct aatatttaca ttatctaaaa taaacaaaac
    24721 agttcagaga gaaatagatg aagctattcg ggatataatt tctcgccatc tatcgtcttc
    24781 ataaacatcg aacactgatg taaactgccc aattcagata ataaaaatct tcgttgaact
    24841 tgactttcag ccggtggggt aattccgtcg gctatctgtt attcaatccc cacgcactgt
    24901 gtttccttaa aattacggtg aaaactcata ccctgctgac cacgagaaaa ttatctggtg
    24961 aaattagtaa tattcatatc attgaaaatg aattgtgcaa ctaagggatg cggcatgatg
    25021 gtcaaaattt tatgtgaata tgagtgactg gccgaaaata ttcagcgtat ttttaatcgt
    25081 ttcaataaaa acagcgagga ttagcagtca acgcttttac tactcaattt tttttacatg
    25141 gtgataaaca aaaaatccac cattaacaga cttcgttcac agtatcaaaa aagctttaag
    25201 aatatattag cagatttctc aaatataatt actgttttat tctcctaatc ttcccataaa
    25261 atgaccgact tacattattt tataattata aacaagtttg attttaaaat catattaatt
    25321 attaacaaga gatttatttt tctaacccca aaagttatgc ttggtcaaag agtaacttat
    25381 aatatccata cccctcatag ggcttacagt ttaaattgcg ggtataaaat acatctattt
    25441 tcaaataaaa taaagcatac tccaattaca acgagtgact aaaataaaaa tatcatccaa
    25501 gtcaatcact gtttgctctg caaaatatgt cagccttctc aaacttacca gcacttaaat
    25561 atttaagcac acaatcattc cttgagggag ttcacttatt tcaagagaaa catttcgtct
    25621 tcccacacaa ctaatccgcc caatatagct aaatttgtac tcagtaatat catttgttat
    25681 gccccccacc ccaaaatatt caagctcctt cacagataaa aaacgacaat ttacaaagcc
    25741 tcattagtta taactatgga aatacctatt ttagtatatc ctataaagtt agaattactt
    25801 cttcttgcac ctaattccat agttgaaaaa caagaataat tttctcctac agctggatcc
    25861 atatagcttc catcaggtct acacaacacc cagtggaggc cgacaggaac tccaataaag
    25921 gatacagcaa cagcttccaa aactctttgg ttagaagata acacatctcg ttgatgtact
    25981 atattacatc ccatgccaat taataagtcc cttgcatcgg gataaataaa actaattact
    26041 tgcgaaaaaa tgttactttc ttcaacgaca taagcattca atccaagaag cctcgtagcc
    26101 atgactatct tatcaggcat agaataaccg gcatccgcga tattatcttt gtgtattctc
    26161 ggattataat taccactagt tattaagtat aaatcccgct cacaacgatt atccaaatca
    26221 agagaactct tccttgtcat ttcggacata gaccctttgt attgtggtat tttatcaacc
    26281 cctaattcct ttgcagcaca gagtaaactg gcagctccac aagaattgtc atacatctgc
    26341 ttcctcgcca aaaaacaacc ttgttcgttt actctattct catttaccct cacatcagtc
    26401 tgatgagata tcatatattc tcttggtata gccccctgca ataaaacccc atataacata
    26461 acacctgtat gaatacatcc ccgtttgcaa ggcttccgtt gttccgacat cgacgagtaa
    26521 ttgcagcaca gtattgcatc cagcaatgcg aactactatg agaattagct gaccgggtcc
    26581 ttattccccg aacgaacttt aaatccaaaa cctcatactc ttttccgtcg tcatcacgtc
    26641 cgtcaccata acaacctttc agcatccacc cttttattaa gagttaatgt ccatcagcat
    26701 cagcaattaa taccgcctga tactgacata tttttgtcgt atttacgtcc ataatcggtt
    26761 attgtgatac aggtcacacc agcttatttg ctattgttac aataaccacc ttggttgaga
    26821 gctgtttttt caactttgtt atcaactccc ccaactcctt ggtcagtaag gctgccgacc
    26881 gagcacagtg aatagatagt atccgcaggt accaccatgc ttacttaata ctcagaaggc
    26941 gaagtgctct ccaattaact ctgccgcact atgcttataa gctgcttttc aatgccgtct
    27001 atcaacatgt ccaattcatt caatctgttg tactggtaat ccaacgcagt aatgatatat
    27061 gcaggtattc tttcatccac tccagtaacg tttcatgcaa agcattaatt tacgcgctac
    27121 ggaacttcac cagttgactg agcaaacgat gtgggaccag tactgcctgc agttcttcta
    27181 ttgttacagc gatttcttta ccaaacagct gaaccaccat caagataaag atagcctgaa
    27241 cattcatcgc accattttgt taccaatggc aaaagattta acgaaccgtg cctgcatcag
    27301 gcggactttt tatccctgct ttgttagctc cctaaccttg tacagagagc ctccataagc
    27361 tcccatatca agcagacatg gttcgcagtt actgacatac accagaaaac tctgacagca
    27421 gactcactta tcctccactt cactcatatc cttattggtg agatgaacct gaatgacatg
    27481 ttttgcgaca taaacgccaa ccagtgtata tttcacctgt agagattcta caatctgtag
    27541 cgttatgcat ctaatgttag gctctatgat gtcagaaatc tgcgagactc tacaccttct
    27601 gcatttacct ttatttatca ccatgctcct cagttaacgg agaacgtgtt cattgcattt
    27661 tgtcacttac atttcgcctt tggtatcggg gggatatagg ttccaacgtt ccgcatgaga
    27721 acctgaaccg gcttcacgcc accttgatac cagatgccgc acaggtagtc aacaa
//
LOCUS       DAJTUP010000049        27596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTUP010000049 DAJTUP010000000
VERSION     DAJTUP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.049"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..598)
                     /locus_tag="OO468_002121"
     CDS             complement(<1..598)
                     /locus_tag="OO468_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="membrane component of a putative sugar ABC
                     transporter system; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR6259234.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHCTRFGNQVYAIGGNATSA"
     gene            complement(585..1607)
                     /locus_tag="OO468_002122"
     CDS             complement(585..1607)
                     /locus_tag="OO468_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6259235.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(1621..3123)
                     /locus_tag="OO468_002123"
     CDS             complement(1621..3123)
                     /locus_tag="OO468_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR6259236.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3263..4219)
                     /gene="ytfQ"
                     /locus_tag="OO468_002124"
                     /pseudo
     CDS             complement(3263..4219)
                     /gene="ytfQ"
                     /locus_tag="OO468_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4529..5059
                     /gene="ppa"
                     /locus_tag="OO468_002125"
     CDS             4529..5059
                     /gene="ppa"
                     /locus_tag="OO468_002125"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR6259237.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5439..5780)
                     /locus_tag="OO468_002126"
     CDS             complement(5439..5780)
                     /locus_tag="OO468_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR6259238.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(5783..9562)
                     /gene="tamB"
                     /locus_tag="OO468_002127"
     CDS             complement(5783..9562)
                     /gene="tamB"
                     /locus_tag="OO468_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR6259239.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9559..11292)
                     /gene="tamA"
                     /locus_tag="OO468_002128"
     CDS             complement(9559..11292)
                     /gene="tamA"
                     /locus_tag="OO468_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR6259240.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11498..12136
                     /gene="msrA"
                     /locus_tag="OO468_002129"
     CDS             11498..12136
                     /gene="msrA"
                     /locus_tag="OO468_002129"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR6259241.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12459..13802
                     /locus_tag="OO468_002130"
     CDS             12459..13802
                     /locus_tag="OO468_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6259242.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(13864..14070)
                     /locus_tag="OO468_002131"
     CDS             complement(13864..14070)
                     /locus_tag="OO468_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR6259243.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14395..14949
                     /locus_tag="OO468_002132"
     CDS             14395..14949
                     /locus_tag="OO468_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR6259244.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15012..15950)
                     /locus_tag="OO468_002133"
     CDS             complement(15012..15950)
                     /locus_tag="OO468_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259245.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16162..16902)
                     /gene="cysQ"
                     /locus_tag="OO468_002134"
     CDS             complement(16162..16902)
                     /gene="cysQ"
                     /locus_tag="OO468_002134"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR6259246.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17092..19035
                     /gene="cpdB"
                     /locus_tag="OO468_002135"
     CDS             17092..19035
                     /gene="cpdB"
                     /locus_tag="OO468_002135"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR6259247.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19161..19541)
                     /locus_tag="OO468_002136"
     CDS             complement(19161..19541)
                     /locus_tag="OO468_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR6259248.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            19630..20490
                     /gene="qorB"
                     /locus_tag="OO468_002137"
     CDS             19630..20490
                     /gene="qorB"
                     /locus_tag="OO468_002137"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6259249.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            20599..21564
                     /locus_tag="OO468_002138"
     CDS             20599..21564
                     /locus_tag="OO468_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6259250.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            21672..22334
                     /gene="ytfE"
                     /locus_tag="OO468_002139"
     CDS             21672..22334
                     /gene="ytfE"
                     /locus_tag="OO468_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR6259251.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22379..23791)
                     /gene="cycA"
                     /locus_tag="OO468_002140"
                     /gene_synonym="dagA"
     CDS             complement(22379..23791)
                     /gene="cycA"
                     /locus_tag="OO468_002140"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR6259252.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24099..24719)
                     /gene="fklB"
                     /locus_tag="OO468_002141"
     CDS             complement(24099..24719)
                     /gene="fklB"
                     /locus_tag="OO468_002141"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR6259253.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            24938..25483
                     /locus_tag="OO468_002142"
     CDS             24938..25483
                     /locus_tag="OO468_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR6259254.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25467..26114)
                     /locus_tag="OO468_002143"
     CDS             complement(25467..26114)
                     /locus_tag="OO468_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6259255.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26326..27117
                     /locus_tag="OO468_002144"
     CDS             26326..27117
                     /locus_tag="OO468_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR6259256.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27127..>27596
                     /locus_tag="OO468_002145"
     CDS             27127..>27596
                     /locus_tag="OO468_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6259257.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRS"
BASE COUNT         6775 a         7345 c         6800 g         6676 t
ORIGIN      
        1 tcgccgacgt cgcgttgccg ccaatagcgt acacctgatt gccaaaacgg gtacaatgcg
       61 caaggaaaat accgataacc accaccgcca gcatcaacag ccccatcgca ctaaggcgac
      121 cgccgccggg gattttccac gcaaggcttg agagcgtgtc ataaatggga tggtttatcg
      181 gaatcgactc ttccgaaacg agatagctga cgccgcgcaa aaagaacatc cccgcaagcg
      241 taatgataaa tgccgggatc ttcagggcgt cgatcagaag ccccataaat gcgccgaagg
      301 cacagcccat caccagcact agcggaaacg ccagcagcgg cgagaggccg aaatcgccaa
      361 ccacttttgc cagaaacacg ccagtaaagg cgatcaccga accgacggag agatcgatac
      421 cgccagagag gatcacaaag gtcatgccaa cggcgattat cccaagaaag gcgttatcgg
      481 tcaggatatt gcagatcacc cttgtggaag caaaaccggg aaactgggtc aggcagtaga
      541 gatagcccag cacaaagacg ccgatggtga tcatcagcgg caagttacgt tttatcatgg
      601 ctacgtactc ctttaatcag actgataaag cgttgcgact gaacaatcag cacacaaagc
      661 accaccaccg ctttcaccac ctggttcatc tccggcggaa agcccgaaag caaaattccg
      721 gtgttcatcc cctgaataat cagcgccccc actaccgaga gcagcaggtt aaagcgcccg
      781 cccatcagcg atccgccgcc aatcaccacc gcgagaatgg cgtccagctc cagccataat
      841 ccggcgttgt tggcatcggc accgcgaata tccgccgcca cgataatgcc cgcaatcgcc
      901 gcacatagcc cgctcaacac gtaggtgagc atgacgatga ttcgcgtgtt taccccggca
      961 ttttttgccg cccgaatgtt gataccaacg acttcgataa acatccccag cgccgttttg
     1021 cgggtcaaca gccagaacag gagaagcgtc agcaccgcga taatgaccgg cgttggcagg
     1081 aacaacagcg atccgctgcc aaaccatgag agatccggcg agttaaacgt gacgatctgc
     1141 ccggcggtga tcagttgcgc cacgccgcgc ccggcgacca tcaggatcag ggtggcgaca
     1201 aacggctgaa ttttgaggat cgctaccagt atgccgttcc acaatcccgc caggatgccg
     1261 gtgcccaggg cgcttaacaa aacaatcggc aggctgaatc ccgcgaccgt catcgcagcc
     1321 gttgtggctc cggcgatagc catcaccgcc cccacagaga gatcgatacc accggtggcg
     1381 atcaccagcg taataccaat cgccagtaac gcaacgggag ctgcacggtt aagaatgtcg
     1441 atagggctac cgaacaaacg cccatcctga agcaccacct gccagaaatg cggggctacc
     1501 aggctatcga ccagcagcac cagcaacagt gccgccagct gcggcatccc cgtcggccag
     1561 tgaaaacgcc tttttggcgg cgtagtgtca gggagagatt gaggcatcac actgttctcc
     1621 ttacgccgca atggcgttca tgatcgccgg aacggaaagc tgtgccagcg ggatctccgc
     1681 cacctgtttg cgatcgcgca tgatgatcac ccggtcggca tagcccacca gctcttccag
     1741 ttcggaggag atcaccagca gcgccagacc gtcagcacac agcgtttcaa tcaggcggat
     1801 gatctcggcg tgggcaccaa catcaatgcc acgggttagc tcatcaagga tcagaaattg
     1861 cggtcgggtc agtagccaac gtgaaagcag cactttttgc tgattgccac cggagagaaa
     1921 ttcaatcggt tgttcagttg aaggtgtgcg aatgccaagc tggcggataa agcgttcggc
     1981 aatctcttgc tgctctttgc gggaaatggg tcgtagccag ccgcgctggg cctggagagc
     2041 gaggatgata ttttcccgca ccgaggcggc ggcgatgatg ccatcggttt tcctgtcttc
     2101 cgggcagaat ccgatgccca gtactgaagc ctgatgtggc gatcgcaggg tttgcggttt
     2161 gcctttgatc aacgccgtgc cgctgtcagc aggtttgata ccgaagatca cttcggcggt
     2221 ttcggtacgt ccggatccca gcaagccagc cagaccgacg atctcgccgg ggcgcacttc
     2281 gagatcaaac ggtgctatcg ttcctttttt gccgtaattt ttgaacgcgg caacgggttt
     2341 gtcgctcaac aatgttcgcc cggcacgctg tagcgcgtgg gtatccagct cgcgccccag
     2401 catcattttt accagttcga tctgcggtag ctcgcgcgtt tcccgacagc ctacgaaact
     2461 gccgttgcgt aagacggtga tccgatcgct gacctgatag acctgatcga gaaagtgagt
     2521 gacaaagatc aggctgacgc cgcgatcgcg caactgacgc atcaggccaa ataatagctc
     2581 catttcctgg gtgtcgaggc tggcggtggg ttcatcgagg atcagcactt tggcggagag
     2641 atcgatagcc cggcaaatgg cgacgatttg ctgcatcgcc accgaaaaac ggttaagcgg
     2701 ttcgcgcacg tcgagggaga aaccgtaaga tgccatcagt tcggtggcgc gcttttccat
     2761 ctctttacgg cgtagaaggc cgaaacgttt gggttcgcga cctatgaata gattatcagc
     2821 aaccgacata ttgggtagca ggttgacttc ctgatagacg gtgccgatac cgagttgttg
     2881 tgcatgggcg gtatttttcg gtgagatagt ctggccttcc agccagatgg tgccgcgatc
     2941 ggcgtggtat acaccagtta atgctttgat tagcgttgat tttcccgccc cgttttcacc
     3001 aagcagcgcc atgatttcgc cacggcgcag gctgaaatca acgttgtcta acgctttgac
     3061 gccggggaaa aatttactta atccttcggt gcggaggatc tcctggtgtt ggtcggtcgt
     3121 catggtttcc cccggtgtaa agcgtgctcc cagtaggcat gataagacgc ggtaagcgtc
     3181 gcatcaggct tcagtgcacc atcgccggat gcggcgcgag cgccttatcc ggcctacgaa
     3241 tcggcatcag gcatagcaac cctcaatacc ccatattttt cttcttctct aactcttctt
     3301 ttgcggtatc aggcaggtaa agggtggatt tggttaacgt cagcttttca ggcatggtgc
     3361 cgtctttttt gtatttctcc agcgcatcga aagcgggtcc cgccatgttc ggcgtcagtt
     3421 caacactggc gttcgcttcg ccatccatca tcgctttgta gatgtccggt acaccgtcaa
     3481 tggaacccgt gaggatatct ttgcccggtt tcaggcccgc ttctttaatt gcctgaattg
     3541 caccaatcac catgtcgtcg ttatgggcgt aaaccatgca gatgtttttg ccgttgtttt
     3601 ccgctttgat aaagctctcc atgacttctt tgcctttact gcgggtgaag tcacctgact
     3661 gcgagcggat aattttgata tttggcgcat tcttaatggc ttcggcaaag cctttcttac
     3721 ggtcaatggc gacgctggcc ccaacggtgc cctgcagctc caccacgttg catggtttgc
     3781 cattcacttc ttttaccagc cagtcaccga tcaacttacc ttcgaggatg ttgtcggcag
     3841 taacggtggt catatagaga gatttgtctt tcacatcgat ggaacggtcg agcaagaaga
     3901 ccgggatttc ggcatctttc gcctctttta ataccggttc ccaacccgtc gcaaccactg
     3961 gagcgataaa gatcgcatcc accccttatg caacgaagga acgtaccgct ttaatctggt
     4021 tttcctgctt ttgctgacca tcggcaattt tcaacgtaat tccgcgtttt tcggcctcac
     4081 ttttcgccac attggtttct gcggcgcgcc agccggattc cgatccgacc tgcgaaaatc
     4141 ctacggttaa tggagcggcc aacgccatag acgacatggc tgccgagact gcagagacta
     4201 caagtaagcg tttccacata gggttgtcct cgtcggggta attattattg gtgaaaagat
     4261 gttcgcgaaa aaactataga caattcgtta tgtaacggat tgcgttacat cacacttcaa
     4321 aaaagtaaat aaaacgttaa tcacaagttt gtaatcgctt tcatctcact atgaaaaatg
     4381 cggctacggt tatggatttt cctgctctgt ataccgtctt aaaactggcg aaaaaggaaa
     4441 atgaagacga aaacaagcaa agacattcgg cgcgagttgg ctataatact tggcacttgt
     4501 ttgccacata tttttaaagg aaacagacat gagcttactc aacgtccctg cgggtaaaga
     4561 tctgccggaa gacatctacg ttgttattga gatcccggct aacgcagatc cgatcaaata
     4621 cgaaatcgac aaagagagcg gcgcactgtt cgttgaccgc ttcatgtcca ccgcgatgtt
     4681 ctatccgtgc aactacggtt acatcaacca caccctgtct ctggacggtg acccggttga
     4741 cgtactggtc ccgactccgt acccgctgca gcctggttct gtgatccgtt gccgtccggt
     4801 tgccgttctg aaaatgaccg acgaagccgg tgaagatgcg aaactggttg cggttccgca
     4861 cagcaagctg agcaaagaat acgatcacat taaagacgta aacgatctgc ctgaactgct
     4921 gaaagcgcaa atcgctcact tcttcgagca ctacaaagac ctcgaaaaag gcaagtgggt
     4981 gaaagttgaa ggttgggaaa acgcagaagc cgctaaagct gaaatcgttg cttccttcga
     5041 gcgcgcaaag aataaataag ttcttctagc gcaataaccc tgaacgccgg gcttcggtta
     5101 gtaagggttt ttttatgccc gcgataaata aactctctat tccaccatca ttattctcag
     5161 cggttgcaag gcttgaacgg taagaacaag caaacccgac caccattttg ctgttcatag
     5221 ccacttgctg gaagttagcc gacctcactc atactcaccg ttcaaagcgt gcccccgttt
     5281 tacccctgca catttgtgaa cgccagcaga gttttttatg cgattgcaga tatggaaagc
     5341 ggctcggaga gaaaagcaaa agatggaaaa gttacaaaaa aaaatcgcga gatacatcgc
     5401 ataaaacaac gccacccgaa ggaggcgtgc ttttattatt acttatccct gtctaaccag
     5461 tcgccgcttt caattagctt taatccatcg acgggtcgtt gataaacgta catccatgca
     5521 ctcccatacg gcgtctgaat caactggcgc gcgtattcac cgcccctggt gcgcaaggca
     5581 tcaagttcgg ccagcgtggc gttgtcaata cgataaactt caccgtgtac cgttccgttc
     5641 cccggaactg cgcctggata gtggcccagg ctatacaact ggtagttatc gatactgaaa
     5701 tcgcccagta actgggcatt ggtcatccag tgactgttgc cttgtttgtg gcgtaaactg
     5761 ccgtagacaa atattcgcat tgctaaaact cgaactgata gagcaaatcc agtgcctggt
     5821 ctacaccaga cacggcttcc agatatagct taggcatcag gcgataacgt aacgtgagtg
     5881 ttgctataga gtcaaatata cccacgccgt atttcacttg cagacctggc aatacatagc
     5941 cgctgaccac tacctgggag gagtcgccta ctcccagggt gtcgagcgct aaattgctta
     6001 cgccaaacgt ctcgccgatt ttacccacaa tctggccact ttgcgcaacc cccagaccaa
     6061 tcagcatcga ggtcattgcc gcactgtcgc tctgatcgct ctccagccct tgtccacgta
     6121 gcaaataaga caatgcagct tgttgcgaca tcgccgggtc agagaagatc tccgctttcg
     6181 gttcgtccgc cagaccagtg acgcgaactc cggcgattac atcgtcttct gtagcatccg
     6241 ggttacgaat agcttcaata ttaagatacg gttgatccgg cggaccagag aacagtaact
     6301 cacccttacg cacaatcaga tcctgaccat aggcatggaa gcgcccttca gggatgttga
     6361 tctgcccgtt caggcccagc ccttgtttgt cctgtacgac gttaagatcg cccgtcagcc
     6421 gcgctttcag gccaaaggcg tcaatgcgca cattgttgcc aacgtggaca atcaggttac
     6481 tgttaatcgg aatcgacgcc gttttcggct cttccggttg caggttatcg ttaagcatca
     6541 ccacatcgct ggagacgcct actgcgcttt ccggcagatc gtgcaccacg atgcgcgccc
     6601 acgggacatc cacgcgacca tcgagggtaa acaggtttgg tgtagcctcg aatacaacat
     6661 ctggcgatac atccattcgt accatcggcg gcacggtgat ccgcacttta ctgcctttcg
     6721 ccgttactcg tgcccgccag ttttcaattt ggctccagtc ggcatcaccg ttcaggtaga
     6781 tttcaccctg ctgggtccgt actgtaccgg caagcgtcga gcgcataccg ttaaagttga
     6841 ccgcaagctg gctcggctgc atatcaaacg gcataaagtt gccgtcgata tccacacccg
     6901 taacctgaag ctgaccaaac aactgcgggc tttgcacatc accacccaga cgcaagttgg
     6961 cactcaccat ccccgctgct ttttccccac gggtaaagat ggggtttatc atcgcaaggt
     7021 tgaagttacg gatattgacg ttgccaccaa gattacggcg gccttgcgga tcggtcacct
     7081 gcacctgtcc atcaaactgg ccgttattgg tcaggcggat ggtccagccc aattcggcac
     7141 ggttgttacg caattccgcc gtcagattca gtgtctgaaa cgccaccggc agcgccgcat
     7201 cgttgacggt ttgcgttacc tgcacgttac gccccgaaag ggtgatactg ccctgcggca
     7261 gcccctcttt cgtggtgtcc caggcaacat ccgctttacc cgtgaagata ccgctggcct
     7321 gagtggtttc tggcataaac ggtttcagca tggcgaggtc gaagcggttg agattcacca
     7381 ccgcacgccc ttcggccccc gcatcgatag tttgcggcac gcacagttcc gcattcgggt
     7441 taagccagca gtgtggcccg atgctgattt tttgctcctt attgcggtaa tccagcgcaa
     7501 tatcgcgggt cagcgaccac gggccgaccg gcgtctggaa gcgggtattg ctaagagttc
     7561 ccttccagcg ttcttctttg cgatcaaaac ttcctgccag attaagctgc ccggagacag
     7621 gctcgccctg aatccgcaac tgtagctcgt gctgcttttc gctgcctttg gcattcaggg
     7681 tgacgaggtt gatatttaca tccggctgcg aaatttgctc aacgcgtacg tcgagtttcc
     7741 cggcgatctg atcggtggat ttaatgtcgc cttccacgcg aacctgcgcc acggaaagtt
     7801 cctgccagcg caggccgcgc gcggtgatat ctgccagtag ttgtggcgct tccaccgtgc
     7861 cgcgtacttt caccagccct ttcgctgtac cgccaagccc cggcagcgcg ttatccagcc
     7921 ccggcgcgtt gatggtggca tcaagattga gatcttttac ccccagctcg cctttcactt
     7981 cggcactgtt tggcccgagt tccagatgaa gcccagggat catccactgc atataactgt
     8041 tgcctttcag cgtgccgtca acgttcactt tgttctgttt gacgttaccg gtcagcttca
     8101 gttctggcac ctccatctgc caggtgccgc cgtacaggct accgcgggtt ttaatcaagc
     8161 cattgagttt cgacggccac tccgggaact ctttggcggt gttaatgcca ttaagcgtta
     8221 gctcaccgcg ccaactaatg gcctgctgcc agtcgagcaa cgccttgagt tcagttttcc
     8281 cttccagcgc cgcgacggtg agtttgtcga gattgacctg ctgttcatta cctttggcat
     8341 caagggtaat ggtggctggc gggatctcct gtcccttcac tgccgtacgc atagagagcg
     8401 tgtaatcggt cattttgccg gtaagtttca gtttcagatc atccgcctga tactgcttct
     8461 caccagtgaa cggccagtaa agctgtttgc tgttcacttc cacgttgagt ggcaatccgg
     8521 cttccgccag tcgcgtctgg gcgcgtaaat ccatatccac cggaccggaa aggttaacgc
     8581 ctatctccag ctgttcgcgc agcgcgccgc ccactttcag cttcaccttt tcacctttca
     8641 acggctccac gttcagtgtg ctgttgaggg tgatatccac cggccagttg tctgacagct
     8701 gcgccgtgcc gctggcgttg acgatcccct ggttggaatc gatatccagg gcgtccagtt
     8761 tagtattgcc gtcaatgctg ctcactttca gcagcatggt gcgcacggtg atgtccgtgt
     8821 cgcccgtcac gcgcagctgc tcgcccttaa actcttcaat gttcaggtta agcggcaaat
     8881 gcacgtcggt catttccggc aataccgggc gagaaaagag atctttcagc gtttcgccga
     8941 gcggcttttc ctccggctgc ggattttcaa ttttcggttc gaccacttct tcctgcgcca
     9001 cttccaccac tttcggcaga gcaatcagca ggcctttcag cgacgtcggt ttcagggtca
     9061 gggttttctc ctgccagttc aggccggagg tgaagtccat caccgacacc gtggtgtcat
     9121 caatcttgat gttgacgttg tccagcgcca cccgtgtcag ggtgatggga tacggcgtgg
     9181 agagatccag cggaccgcta tcttcctctt cttcaacctg ttcagaagga ggcatttttt
     9241 tactgtcgat gttgacctga atgtctttca gcgccaggtc attaatacaa acgctactgt
     9301 tccacaggca ctcaagaccg acccccagat gcagattacc cgcttttacc gccacgccta
     9361 gctgctcata acgaacgtca gacaaggtga gatcgcgcca gccgccggtg accttgccaa
     9421 tatccagtcc tggcacccag cgatccgccg ctttaaatac aagatgcagg ccgctggtgg
     9481 tgcccaccag aaacgccacc gatcccagca acagtaagat aacgataacc acgccgaggc
     9541 tgattttttt ccataaactc ataattctgg ccccagaccg atgtaaaact gtaacccgtg
     9601 ttcgtcttta tccgcgaccg gtacggcaaa atcgagtttg attggcccga ccggcgattc
     9661 ccagcgtacg ccgaccccgg taccggtttt aaagtcgctg cggcgaatat cgcttaccgc
     9721 ttcgccacta tcgacaaaca ccgcgcccca ccattttccg gtcacgttgt actggtattc
     9781 cagcgatccg gttatcaact tcgaggcccc tttcaggtca ccgttggcgt atttcggggc
     9841 gatagatttg tatttgtagc cgcgaatact gcgatcgccc ccgacgaaga aacgcagatc
     9901 cggcggtact ttgtcgaaat caccggtttc aatccagccc agcgtgccgc gtgtcacaaa
     9961 gcgatggcga tcgtacagtg tgcggatcca gacgtgctgc gcctggaaaa cggagaaatc
    10021 gacatctgaa ccccaggccg tgttggagta gtcgatagag tagcgttgcg agtctcccca
    10081 ggttggcatc aggccaccac gagaacgcgt gcggctaatc atcaccccag gataaaacag
    10141 catcgtggtg ttggtaattt caccctgggt aaagtggtcg aggctccagc gcaggttaat
    10201 ggcacgctgc cagccgctgg agagatccca gtagcgagaa gccaccagcg tagtggagtc
    10261 agattcggta tcgttcaggt cagtgcgctt aaaaccgccc tgcaccaaat aatattgttc
    10321 cagtggattc ttcagcagcg gcattttata gctgaagtcg agggtctgtt ccggcgcgga
    10381 aatactggta ctggtggtca aactgtgacc gtatgagttc atccacggct ttttccacgt
    10441 cgctttcacg cgcggtccca cgtccgtaga gtaaccgacc ccggtttcga tggtgttttc
    10501 tgttcgcggc gaaaccacgc ccgtcaatgg taatactttc gtttcgcgcg ctttatcaaa
    10561 ttgtggagcc accaccaccg agttaaacca gccggtagca gaaagtcggc ggttcagttc
    10621 cgccagatct ttcgattcat actcatcgcc ctctttaaac ggcaccagat tttgcaggta
    10681 ttcatcgcgg atttgtgatc cttcaaaggt cacatgccca aagcggtaac gttcgccact
    10741 gttataatca atatcccaga aggctttatg caggccgagc gcaatgccca gctgcgcttt
    10801 ggtaaattcg ctatcgaaat aacctttacg caacgcaata ctggttaagg actttttgaa
    10861 attttcataa tcgccctggt tcagtaccgt accaatagcc gggcgagtat cgagcaattt
    10921 caaatagtct ttatcggtcc gcgcgccgcc gcgcaatacc acatcggtgc cgccaattaa
    10981 caccggcaca cctggcgtga ctttggcgat caatacctgc cgccctttct ttggcggtgg
    11041 acggagatca aattcaatgg tcggctggta ataacccagc gctttcagac cttcacggat
    11101 ggcatcatcg acgcgtgcgc gaaagcgacg gtctggcgtc acttcatcac tttcaatcgt
    11161 agaaagctgc gcacgaacgt tcttttccag ctgtcccgat aacccctcga cctgtagacg
    11221 gacgttcgcg gcgacggcag atccgcttaa gcagagtaag cttacacagc ataactgtcg
    11281 gatatagcgc acattttctc ctgaatatcc ttttttcctg cccctggaac gccgttaaac
    11341 ggcttaacaa aaatccagta atatggatta aaaagcagac taaaccccaa atatttctta
    11401 tgttttactt tagacctatt cacggtggtt attgtgtgca aatacgcctc ttgttacaac
    11461 cttaacccca atgaccgatt ttcgggagag cgacaccatg agtttatttg ataaaaagca
    11521 tctggtttcc cccgccgatg ccctgcctgg acgtaacacc ccgatgcccg tagccacgct
    11581 gcatgcggtc aacggtcact caatgaccaa tgtacctgac ggaatggaga ttgccatttt
    11641 tgcgatgggt tgtttctggg gtgtggagcg tctgttctgg cagttacccg gcgtttacag
    11701 caccgccgca ggctataccg gcggctatac gccaaatccg acttatcggg aagtgtgctc
    11761 cggtgatacg ggtcatgccg aagcggtacg cattgtttac gatccttccg tcatcagcta
    11821 tgagcagttg ctacaggtat tttgggagaa tcacgatccc gcccagggaa tgcgtcaggg
    11881 caatgaccac ggcacgcagt atcgttcagc gatttatccg ctgaccccag aacaggatgc
    11941 cgcagctcgc gccagtctgg aacgttttca ggcggcgatg cttgccgccg atgacgatcg
    12001 tcacatcacc acggaaatcg ctaacgccac accgttttat tatgccgaag atgaccacca
    12061 gcaatatctg cataaaaacc catatggtta ttgtggaatt ggcggaattg gcgtctgtct
    12121 gccgccggaa gcatagcgtt acgggtacaa atgtagtttg ttgataaagt gctctttgtc
    12181 tatgcctgat gcggcttaaa cgacttacca ggcctacaaa atcgcccaaa ttcaatatat
    12241 tgcaaggact gcgtaggcct gatcggcata gcgcatcagg cagtattgca tttatcagca
    12301 gtctgaattt taaccctctg gcgactttac agtaccttac gctatactag ccactgaaaa
    12361 tgccggttca ctttcttcga atcggctttc aatgtgtatt tcacacaaat taatcaactt
    12421 tcccttccga gggtctggcc tgaaaggtcg gataagatat gttaaacagt attttagtca
    12481 tactctgctt gatcgctgta agtgcgttct tctcgatgtc cgagatctcg cttgccgcct
    12541 cacgcaaaat caaacttaaa ctgctggctg atgaaggcaa tataaatgcc caacgcgttc
    12601 tgaatatgca ggaaaatccc ggcatgttct ttaccgtggt ccaaatcggt ctgaacgcag
    12661 tggcgattct cggcggtatc gtcggtgatg cggcattttc tccagctttt cacagcctgt
    12721 tctcccgcta tatgtcggca gagctctctg agcaactgag ctttattctc tctttctcgt
    12781 tagtgactgg catgtttatc ctgtttgcgg atttaacccc gaaacgcatc ggtatgattg
    12841 cgccagaagc ggtggctttg cgtatcatca acccgatgcg cttctgcctg tacgtttgca
    12901 ccccgctggt gtggttcttc aacggcctgg cgaacatgat cttccgtatt ttcaaactgc
    12961 caatggtacg taaagatgac atcacttctg atgacatcta cgcggtagtg gaagccggtg
    13021 cgctggcggg cgtgttacgt aaacaggaac acgagctgat tgaaaacgtc tttgagctgg
    13081 aatcccgtac cgttccgtct tcaatgacac cgcgtgaaaa cgtgatttgg tttgatctcc
    13141 acgaagatga gcaaagcctg aagaataagg tggcggaaca tccgcactct aagttcctcg
    13201 tctgtaatga agatattgac cacatcatcg gttatgtcga ttctaaagac ctgctgaacc
    13261 gcgtgctggc taaccaaagc ctggcactga acagcggcgt acaaattcgc aacacgctga
    13321 ttgtgccgga tacgttaacc ctttcagagg cgttggaaag ttttaaaacc gcaggtgaag
    13381 acttcgcggt gatcatgaac gagtacgcgc tggtggtggg gatcatcacc ctcaacgacg
    13441 tgatgaccac gctgatgggc gatctggtcg gtcaggggct ggaagagcag attgtcgccc
    13501 gtgatgagaa ctcatggctg attgacggcg gcaccccaat tgacgacgtc atgcgcgtgc
    13561 tggatattga cgagttcccg cagtcgggca actacgaaac catcggcggc tttatgatgt
    13621 ttatgctgcg taagatcccg aaacgcaccg attcggtgaa attcgccggc tacaaatttg
    13681 aagtggtgga tatcgataac taccgcatcg accagctgct ggtgacccgg atcgacagca
    13741 aggccaccgc cctttcgcca aaactgcctg acgctaaaga taaagaagaa agcgtcgcgt
    13801 aacccccaga aacatcaacg gctcctgaat caggagccgt cttattactg catagcactt
    13861 tggttaagcc atctctgttt gcagacgcat aacctgacgg ttaacttcgg acatcactga
    13921 caaatgcagc ttatccttca cttttgggat aagaatctta cccttatcaa attcaaaagc
    13981 gccaatgtcc ttgatgtata accgtccacg gaacaggatc tttacgtact tcgccacctg
    14041 aagtgggttg taccgttgga aaattttcat tgttatctcc tgctgagtat tacgcctgtg
    14101 cggtgccaca atcggcccaa ctattatgag gcgcaaattt taatgcttag tgactataga
    14161 ctatccgggc aatgtttcca ccgcgtataa ctttttttac ctaaaggtta caattattca
    14221 gaattatctt tttaccgaag cgcggtcttc agtataagca ttcatttttc atatgatttg
    14281 tgcgcttgac cgcaaactgg catcacactt gcgggaaatt cgataaatag cacatatgat
    14341 taaaactcag acccaagtgg tcggatcacc tgcatatcat aagaaggaaa caccatgacc
    14401 ctacgcaaga ttctggcact cacctgcctg ctgttgccga tgatggcttc cgcacatcag
    14461 ttcgaaatcg gtcagcgagt gccgccgatt ggcattaccg atcggggcga gttggtgctt
    14521 gataaagatc agtttagcta caaaacctgg aacagcgcgc agttagtggg aaaagtgcga
    14581 gtactgcaac atattgctgg tcgcacctct gcaaaagaga aaaacgcgac gctgattgaa
    14641 gcgattaaat cagcgaagtt accgcacgat cgttaccaga ccaccaccat tgttaacacc
    14701 gacgacgcga ttccgggttc cggcatgttt gtgcgcagca gtctggagag taataaaaag
    14761 ctttatccct ggtcgcagtt tattgtcgat agcaatggcg tcgcgcgcgg tgcctggcag
    14821 ctggatgaag aaagttccgc tgtagtggtg ctggataaag acggtcgcgt gcaatgggcc
    14881 aaagacgggg cgctcacacc ggaagaggtg cagcaagtga tggacctgct gcaaaagttg
    14941 ctcaaataat ggcaataaca taaattctgg aaaaataatt ttcccctatc gttatctatg
    15001 ggatagggga attaagtctc tgagttctta caccgaacta atactggtaa cgctagttgc
    15061 tcttttttaa acgcctcaat ggtcgattta tatttctcgc aaatcttttc tttggtaata
    15121 ttactatctg cggccagatt cgcacttact atcgcatcca cttgttcggc taattcgact
    15181 tgttggcaac ctgcattaac cagaaaatta taaataagag agtaatcttc atcctccaaa
    15241 acaacagagg aaccattacg aaaacgttca tcccctccat agattttaag cgcaaagccc
    15301 aaagattcta tgactatggc aaaattatca tcaattattt tttcgtaaat ataccatcca
    15361 tcgcattctt taattccacc gcctggtatt tggagttcaa atttaccccg ttttaatttg
    15421 cttactacat cctccaataa ggcataaccg aaagatacgt caccaaacgc ctggcgaata
    15481 taacctttaa tcctttttgg tatgtcagac tgttttttct gcaaaatatc acaggcatac
    15541 aatcttctgt tttgttctaa cggaaactcg cggtttttga aatccatgaa tgagttatac
    15601 tcatttattg ttatgggaat ttcttcgtat tcaaacccac tgcctcgcaa tgttattgta
    15661 tattcagcac cgttatcttt aaggctgaaa ctgataagaa aattatccat ggcattaaca
    15721 gtttcatttt ttgatgaaag cacttgataa ctatttttat caatctgtgt tgcgcaacga
    15781 aaggactccc gcaaagttaa cagactatca taattcgaaa cccaatcttt tttcttaaat
    15841 gcatgctgaa tattattaaa aaaatcgttt gaatgtgttt ttgtatgcga tgactttaca
    15901 aataactcca ccgcaccaat aaaattattg tgtctgatcc tgggtaacat atggctacct
    15961 tattttattt ggttatttta tgtcgccatt aaatatctca ttatagaagt aaactatcaa
    16021 aaaacgctca ttattaaaat gtaacttact agcaattgaa tttttgcact ttaaatacaa
    16081 tctcttttta tttataactt tttaggttgg gataaatcag gatggcagaa tcaggaaata
    16141 cactgtttct gccatctgaa tttagtaaat ggacactctg aaccccggat tcaggaacga
    16201 ctcgcgcgga gtgtaatcca gcggtttacc ctgccagtcg tgaacgtgcg ctccggcagc
    16261 tgcagccaca gcatgtccgg cggcggtgtc ccaaatattc gttggtccga agcgcgggta
    16321 cagctgcgcc tgtccttccg ccaccaggca gaatttcagc gaagagccga tggacgtggt
    16381 ctgatgttcg ccaagctgtt gcagatactc tttcagctcc gcatctgcat gggaacggct
    16441 gataaccacc agcggcgggc gcgcatcgcg gacctgaatc agcttgcgca caccgcactc
    16501 ttctttccag gccttgcctt ctgccgcgct gtacattacg ttcattaccg gcgcatacac
    16561 cacacctaaa atcggtttgc catggtcaat gagcgcaatg ttaacggtga attcgccatt
    16621 acgtttaata aactctttag taccatccag cgggtctacc agccagtaac gctgccagtg
    16681 ctgacggact tcccaaccgg gaggatcttc ttcagaaagg accggaatat ccggtgccag
    16741 cgtacgtaaa ccgtccatga taacggtgtg agcggcaata tccgctgccg ttaccggaga
    16801 attgtccgct ttgctgacga cgtccatcgg tttcgtcccg tcgtagacct gcataatggc
    16861 atcgcctgca ttccgtgcaa gctggcatac ttgatctaac atttctccac ctcgtctctg
    16921 tgagcggtgt taacttattg ttttacttat accctatcgt taatgaatgc gccaactgtg
    16981 atagtgtcat cattttcaaa gcgtaaaatt gtggcattct tcactgttct ataagtaagg
    17041 cgtttattct cccttttctt tcgtattccc gatgataaaa ggatgtccct gatgattaag
    17101 tttagcgcaa cgctcctggc cacgctgatt gccgccagtg tgaatgcagc gacggtcgat
    17161 ctgcgtatca tggaaaccac tgatctgcat agcaacatga tggatttcga ttattacaaa
    17221 gacaccgcca cggaaaaatt cggactggta cgtacggcaa gcctgattaa cgatgcccgc
    17281 aatgaagtga aaaacagcgt actggtcgat aacggcgatt tgattcaggg gagtccgctg
    17341 gccgattaca tgtcggcgaa aggattaaaa gcaggtgatg ttcatccggt ttataaggcg
    17401 ctgaatacgc tggattatac ggtcggtaca ctcggcaatc atgaatttaa ctacggtctg
    17461 gattacctga aaaatgcgct ggcgggagcg aaattccctt atgtaaatgc caacgtcatt
    17521 gacgccagaa ccaaacagcc aatgtttaca ccgtatttaa ttaaagatac cgaagtggtc
    17581 gataaagacg gaaaaaaaca gacgctgaag attggctata ttggcgtcgt gccgccgcaa
    17641 atcatgggct gggataaagc taatttatcc ggaaaagtga cggtgaatga tattaccgaa
    17701 accgtgcgca aatacgtgcc tgaaatgcgc gagaaaggtg ccgatgtcat tgtcgttctg
    17761 gcgcattccg ggctgtctgc cgatccgtat aaagtaatgg cggaaaactc agtttattac
    17821 ctcagtgaaa ttccgggcgt taacgccatt atgtttggcc atgctcacgc cgttttcccg
    17881 ggtaaagatt ttgctgatat cgaaggggct gatatcacca aaggcacgct gaatggtgtt
    17941 ccggcggtaa tgccgggcat gtggggcgat catcttgggg tggtcgactt acaactcagt
    18001 aatgacagcg gtaaatggca ggtgacgcag gcgaaagcgg aagcacggcc gatttacgac
    18061 atcgccaata aaaaatccct cgcggcggaa gacagcaagc tggtagaaac actcaaagcc
    18121 gatcacgatg ccacacgcca gttcgtcagc aagccaatcg gtaaatctgc cgacaatatg
    18181 tatagctatc tggcgctggt gcaggacgat ccgaccgtgc aagtcgtgaa caacgcgcaa
    18241 aaagcgtatg tcgaacatta cattcagggc gatccggatc tggcaaaact gccggtgctt
    18301 tcagctgccg caccgtttaa agttggtggt cgcaaaaatg acccggcaag ctatgtggag
    18361 gtggaaaaag gccagttgac cttccgtaat gccgccgatc tttatctcta ccccaatacg
    18421 ctgattgtag tgaaagccag cggtaaagag gtgaaagagt ggctggagtg ctccgccgga
    18481 cagtttaacc agattgctcc taacagcacg aaaccacagt cactcatcaa ctgggatggt
    18541 ttccgcactt ataactttga tgttattgat ggtgtgaatt atcagattga tgttacccaa
    18601 cccgcccgct atgacggcga gtgccagatg attaatgcca atgcagagcg gattaagaac
    18661 ctgaccttta atggcaagcc gattgatccg aacgctatgt tcctggttgc caccaataac
    18721 tatcgcgctt acggcggcaa atttgccggt acgggcgaca gccatatcgc ttttgcttca
    18781 ccggatgaga accgctcggt gctggcagcg tggattgctg atgagtcgaa acgtgcgggg
    18841 gaaattcatc cggcggcaga taacaactgg cgtttagcac cgatagctgc cgataagaaa
    18901 ctggatatcc gtttcgaaac ttccccgtca gataaagccg cagcgtttat taaagagaaa
    18961 gggcagtatc cgatgaataa agtcgcgacc gatgatatcg ggtttgcgat ttatcaggtg
    19021 gatttgagta agtaaaacac ttctttttcg gcctataaat caaccgcatc cggcatttat
    19081 tggcggatgc gatgctgccg tatcttatcc accctacaag ccatgaaccg tagaccggat
    19141 aagcgcagcg catccggcag ttatgccgca cgttcatccc gcaccgccaa cacctcgggc
    19201 aaattcaact caatccagtc cgccagtgca gcaacctttt cgctcacctg ctcgcccagc
    19261 ggcgtgaggc tatattccac atgcggcggc accaccggat acgcgatacg gttaagaaaa
    19321 ccatcctgtt ctaacgcctg taacgactgc gcaagcatct tttcactcac cccaccgatt
    19381 ttgcgccgca ggtcgctaaa gcgatgagta ccttcgcgta gcgccaccag aatcaacacc
    19441 ccccaacggc tggtgacgtg tttcaacacc tcgcgcgacg ggcactgttc cgcaaagaga
    19501 ttaccctctt tcagttgttg tgacaggcta acctgactca tttcatactt acctttttgc
    19561 acgtacttac taaaagtaag tttaggtgtt agcgtattta aacacaagac aaaacgatgg
    19621 agacttccca tgatcgctat tactggtgcc actggccaac ttggtcacta tattattgaa
    19681 tccttgatga aaacggttcc tgccagccaa atagtggcta tcgttcgtaa tccggcaaaa
    19741 gcccaggccc tggcagcaca aggcattacc gtgcgtcagg ctgactacag cgatgaagcc
    19801 gcactgacat ctgcacttca gggagtggaa aaactactgc tgatctcttc cagcgaagtg
    19861 ggtcaacgtg ccccgcagca tcgtaatgtt attaatgccg caaaggcggc tggcgtgaaa
    19921 tttatcgctt ataccagcct gctacatgca gatacctccc cgctcggcct cgccgatgag
    19981 cacatcgaga cggagaaaat gctggctgat tctggcatcg tttacaccct gctgcgcaac
    20041 ggctggtaca gcgaaaacta cctcgccagc gccccggcag cactggaaca cggcgtattt
    20101 atcggtgcgg ctggcgatgg caaaatcgcc tcagcaacgc gggcagatta tgcgacagct
    20161 gcggcacgcg tgattagcga agccggtcac gaaggcaagg tttacgaact ggcgggcgat
    20221 agtgcctgga cgttgacaca gttagcggca gagctgacca aacagagcgg caaacaggtt
    20281 acctatcaaa atctgagcga agccgatttc gccgcggcac tgaaaagcgt cggactgccc
    20341 gacggactgg cggatatgct ggcggattct gacgttggcg catcgaaagg tggtctgttt
    20401 gataacagca aaacgcttag caaattgatt ggctgcccaa cgacaacgtt agccgaaagc
    20461 gtaagccatc tttttaatgt taataactag ttaattaaag tggcatcctc ccgcatcctc
    20521 tctgataatg acgggatgcc gggagcaatc atgtctgctt cctgaacgtt tcttctgaca
    20581 gaccaatgga tgccagtaat gattagcggc gtgctgtacg ccctgttagc agggttgatg
    20641 tgggggctta tttttgtcgg gccgttgatc gtgccggaat acccggcgat gttgcagtcg
    20701 atggggcgtt atctggcgtt agggttaatt gcgctaccca ttgcctggct gggacgcgtg
    20761 cgtctgcgtc agttggcgcg tcgggactgg cttaccgcct tgatgctcac tatgatgggc
    20821 aacctcattt attacttctg ccttgccagt gccattcaac gtactggcgc gcctgtttcc
    20881 acgatgatta tcggcaccct gccggtggtg attcctgtct ttgccaatct gctttatagc
    20941 cagcgcgacg gcaaactcgc gtggggaaaa ctcgccccgg cactgatttg tattggcatc
    21001 ggcctggcga gtgtgaatat tgctgagtta aaccacggac tccccgattt tgactgggca
    21061 cgttatacct ctggcatcgt gctagcgtta gtttccgtgg tctgctgggc atggtatgcc
    21121 ctgcgcaacg cccgctggct gcgggaaaat cccgacaaac atccgatgat gtgggcgacg
    21181 gcgcaggcgc tggtcacgct gccggtttct ctcatcggct atctcgtcgc ctgttactgg
    21241 ctgaatatac aaacgccgga cttctcctta ccttttggcc cccgtccgct ggtgtttatt
    21301 agtctgatgg ttgcgatagc cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc
    21361 gccagccagc gattaccgac agtgattctc gggccgctga ttgttttcga aacgctggca
    21421 ggtttgctgt acaccttttt actccgccag caaatgccgc cgctaatgac gctgagcggt
    21481 atcgcgctgt tagtgattgg cgtggtcatc gcggtcagag caaaaccaga aaaaccttta
    21541 actgaatctg tctcagaaag ttgacatgct ggcagtgagt taaataagcc tctgctacgt
    21601 aagggttata gcttttgcct taaaggtgta tttaaaatac atcttatctt attaagaatg
    21661 aggtatcagc tatggcttat cgcgaccaac ctttaggtga actggcgctc tctatccctc
    21721 gcgcttcggc tctgtttcgt aaatatgata tggattactg ctgtggcggt aagcagacgc
    21781 tggcgcgcgc ggcggcacgt aaagaactgg atgttgaggt cattgaagct gaactggcaa
    21841 agcttgctga acaaccgatt gagaaagact ggcgtagcgc cccgctggca gaaatcattg
    21901 accatatcat cgtgcgctac cacgatcgtc accgcgagca gctgccggag ctgatcctgc
    21961 aagcgactaa agtcgagcgc gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa
    22021 aatacctgac catgctgcat gaagagcttt ccagccacat gatgaaagaa gagcagatcc
    22081 tcttcccgat gatcaaacaa ggcatgggca gccaggcaat ggggccaatc agcgtaatgg
    22141 aaagcgagca cgatgaagcg ggcgaactgc tggaagtgat caaacacact accaataacg
    22201 tcacaccgcc gccagaagcc tgcaccacct ggaaagcgat gtataacggc attaatgaac
    22261 tgattgatga cctgatggat cacatcagtc tggaaaacaa tgtactgttc ccacgcgccc
    22321 tagcgggtga gtgatctaaa agctggatgg cattgcgcca tccagcatga taatgcggtt
    22381 atttccgcag ttcagcagcc cgcttcttac caataaacag ccagcccaac cccagcgcga
    22441 taaaccacag cggggtaacc agcagcgcct ggcgagtgtc atcttccagt gtcagcaaca
    22501 cgaccacgaa cacaaagaac gccatacata cccagcacat cagcttgccg agcggcatct
    22561 tgtagatcga cttttcatgc agatgaggac gctgtttgcg gtacaccagg taagagcaaa
    22621 ggataatcgt ccagacgaac ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg
    22681 cgccaatcac gctaggattc acatacaaca tcaccacgcc gccgagcaga cagatacacg
    22741 agaaggtcaa ccctttcgcg ggtactgcgc gcttagaaag tttagcgaac gctttcggcg
    22801 caacaccttc ctgcgccaga ccaaacagca tacggctggt agagaagaca ccgctgttag
    22861 cggaagacgc cgcagaggtc agcaccacaa agttgatcac gctggcagcc gcaggcaaac
    22921 ctaccaacac aaacagctca acgaacgggc ttttctccgg gactaccgaa ctccacggtg
    22981 tcacggacat aatcacaatc agcgcgaaga cgtagaacat aatgatacgg atcggaatgg
    23041 agttaatcgc gcgtggcagt gatttctccg gatctttggt ttccgcagct gttgtaccta
    23101 ccagctcaat ccccacgaaa gcgaaaaccg ctatctggaa tccggcaaag aagccactta
    23161 aacctttcgg gaaccagccg ccgtcattcc acaaatgcgc gaatgacgct tccacaccgg
    23221 tcggtgactg aaagtgcatc gccaccatga ccaggccgac gacaatcagc gacacgatgg
    23281 cgacgatttt gatcatcgca aaccagaact ccatctcacc gaacattttc acggtggcaa
    23341 ggttgagcgt cagcagcagc actatcaccg ccagcgaggc gacccagtcg gagagatcgg
    23401 ggaaccagaa ctgagcataa gccgtgatcg ccaccacgtc tgccataccg gttacaaccc
    23461 agcagaacca gtaagtccag ccggtgaaat atcccgccca cggccccagt aaatcggaag
    23521 cgaagtcact aaaagatttg tattccagat tcgaaagcag caattccccc attgcccgca
    23581 tcacgaaaaa gagcataaaa ccaatgatca tataaacgaa aatgatcgac ggcccggcaa
    23641 ggctaatcgt tttgccggac cccataaaca acccagtacc aatggcacct ccaatggcaa
    23701 taagctgaac atgtcggttt gtgagattgc gccgtagcga ctgttcagcc ggagcctgat
    23761 catcggcaac gacttttacc tgatctacca tgttttttct tcctgtacct gtctgtgttg
    23821 ttcaggctct acggccttta gtcagtctat gatacgacga atctgtatcg ggactcatcg
    23881 atattaggta acaatacgcg ggatgaatac taagatttag atctaatgtt aattttatgt
    23941 ttaaagtgag tgtcatatca cggtgataac gcgaaacagc tcacaaaaat acacgcttac
    24001 ggggtctttg caacacaaaa tagcgccgtt tggcaaactg aagggtttat tgctgaatgc
    24061 ctgctcccct ctggttcgag gggaacagaa tgcgctgctt agaggatttc cagcagttcg
    24121 acttcaaaca ccagggtgct gaacggaggg atggatgcac ctgcgccgcg ctcgccatat
    24181 gccagttcct gcgggatagt cagttcccat ttagaaccta ccggcatcag agtcagtgct
    24241 tcaatccagc cagggatcac gccattaacc gggaattcag cgggttcacc acgagcaacg
    24301 gagctgtcaa acacggtgcc gtcgatcagt ttaccggtgt aatgaacacg aacgcggtcg
    24361 gtacgtgccg gaattgcgcc ttcaccctgg ttgatcacgc ggaattgcag gccagattcg
    24421 gtgctattca caccttcttt tttggcgttt tcttccaggt atttcacgcc ttcagcagcc
    24481 atcgcctgga aacgctgacg acgaacggca tcggcgcgtt cgtggatttc acgcagcgcg
    24541 cgatgcacca catcaaccgg cacagccgga tgtttgcctt ccagcgcatc ggcaatacct
    24601 gcaaccagtg cttctggcag cagcccttcc aggccagatt cactcagttg ttgccctacc
    24661 tgcaaaccaa tgccgtagct tgcttgcgct tcgatggtgt caaaagttgg ggtggtcatc
    24721 gtatttcctt tcttagggta taaaagtagc gggcagcata tcagccacac ttctttgggt
    24781 aaaactttgt cgcaggaatg atgacaaatc ccggggaaaa agaaacaata gcaatatccc
    24841 gtatgaatat aataaaggcg ggtttaccac ggacatcata gcgttagctt attcataggc
    24901 tatgattgag gaacaagacg tggagcagga ggaaaccatg cccgggcgct ttgaactaaa
    24961 accaaccctg gagaaagtct ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc
    25021 gccgatgcat cgtcggggga tcattattgc cgccattgta ttggtggtcg gatttctgct
    25081 tccatctgat gatacgccca acgcgtcggt ggtgacgcgt gaagcgcagt tagatattca
    25141 gtcgcaatca caaccgccga cagaagaaca gccgcaaact acccaaacgc aaccattcca
    25201 gccagatagc ggaattgata atcaatggcg ctcttaccgt gtcgaacccg gtaaaacgat
    25261 ggcgcagctg ttccgcgacc acggactgcc cgctaccgat gtctatgcaa tggcgcaggt
    25321 agaaggcgcg ggtaagccgc tgagtaattt gcaaaatggg cagatggtga aaatccgcca
    25381 gaacgccagc ggcgtggtga cgggcttaac cattgatacg gggaataatc aacaggtcct
    25441 gtttacccgc cagccggatg gcagttttat tcgtgcgcgg tagatgaagg gtcaaccaaa
    25501 agccagctaa ggattgtctc tttgatgacc ttacgtctgg cttccagtgc ctggggggaa
    25561 accatactga tatgataaac taggccaagc gtatgctggt tgatcaaata atatccccca
    25621 agcgcagcaa tattaatatt cacctgcaat gggtcgatac ctggcttaaa aatattgtat
    25681 ttcttccctt catccaataa ggattccatt aactgcaagt gagcatggtt aatttctaag
    25741 agacgctgtg attttgcata atgcacacct ttactttgat tttcactatg cacaattttt
    25801 aaaaaccaag gattagcaat ataataatcc catgtaaaat caataatttt ctcaatggct
    25861 ttttgtggcg gaagatctga gaaatcaagg gccttttctt tttgtcgaat atcgttccag
    25921 gtatattcca gcacttcgat aaataaattt tctttattac caaagtggtg ataaaccgtc
    25981 tgtttactac accctgctgc ctttacaata ttatctactc tggccccttc ataaccatat
    26041 tcggcaaatt cattaaccgc acatagcagc aatttttcct tcaggctctg tacgttgctg
    26101 gtatcaatat acataacgct tccccacgac ctcttcgtgg tgactatcat aacctgattt
    26161 gatttcagct tcagaaaaaa gggcaaaaac ttgtactttc atcaggttat caaatgtcct
    26221 gtgatcgcca tcgcatatca accaactaga tggtttatct ttttatcctc cagtcgcaag
    26281 ctaatcatca ccaaggaaac aaaattcaaa aaagagagct attgtatgaa atccactcgt
    26341 cctgttgccg tgattaccgg agccactcgc ggtattggaa agggctgtgc gcaggagctt
    26401 gctcgtggcg ggtttaacct gctgattaac gatcgtcccg atgccgacag cgtagaaaaa
    26461 ctgcatatca cacaacaaga atgtttggcg gaaggcgtgg aagtgatttg cttccctgct
    26521 gatgtcggtg atctatctct gcatgaggag atgctcgacg cggcgcaaaa ccagtggggg
    26581 cggctggact gtctgctcaa taacgcgggg atttcagtga aaaaacgcgg tgacctcctc
    26641 gacctcgaac cagacagctt cgatcaaaac attgccatta acacccgcgc gccgttcttc
    26701 cttgctcagg cattcagtaa gcgcttactg gcacagccga aaccggaagc ggagctgccg
    26761 caccgctcga ttattttcgt cagttccatc aacgccatca tgctggcgat gaatcgcggc
    26821 gaatacacca ttgccaaaac cgccgtttct gccgctgctc gtctgtttgc tgctcgcctt
    26881 tgtaatgaac aaattggcgt ttatgaagtg cgccctggtc tcatcaaaac tgatatgact
    26941 attccggcaa cggcttatta cgacgaactg attgccaaag gtctggtgcc gtggggacgt
    27001 tggggttatc cggctgatat tgcttccacc gtccgcgcga tggcggaagg taaactgatt
    27061 tacacctgtg gtcaggcggt cgcaatcgac ggcggcttaa gtatgccgcg cttctgaggc
    27121 tgaatcatga gagatctgtt acaacgcccg gatttattca gtatcaatac cgcgacactg
    27181 ggctataaaa cgccgctacc cgccattatt gatgcctgcg ccgcacgcgg tattggcgca
    27241 attgccccct ggcgcaggga gttgcagggt gaagatctcc agcaaatcac ccgccagctt
    27301 gccgccagta acatgagcgt ttccggtttg tgccgtagca cttactacac cgcgccgaca
    27361 ctggcggaac gcaaactggc gattgatgat aaccgccggg cgctggacga cgcagcggtg
    27421 cttaacgccg cctgttatat gcaggtggtt ggcggtctgc caacgggcac taaagatcta
    27481 tatgaagcgc gagaacaagt gaaacaaggg attcgccagt tgctgccaca ttcgaaagat
    27541 gtcggcgtgc caattgcgct ggagccactg cacccgatga cagctgccga tcgctc
//
LOCUS       DAJTUP010000050        27457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTUP010000050 DAJTUP010000000
VERSION     DAJTUP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.050"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..360)
                     /gene="araH"
                     /locus_tag="OO468_002146"
     CDS             complement(<1..360)
                     /gene="araH"
                     /locus_tag="OO468_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6259258.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVN"
     gene            complement(375..1889)
                     /gene="araG"
                     /locus_tag="OO468_002147"
     CDS             complement(375..1889)
                     /gene="araG"
                     /locus_tag="OO468_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR6259259.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(1959..2948)
                     /gene="araF"
                     /locus_tag="OO468_002148"
                     /pseudo
     CDS             complement(1959..2948)
                     /gene="araF"
                     /locus_tag="OO468_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            3745..4248
                     /locus_tag="OO468_002149"
     CDS             3745..4248
                     /locus_tag="OO468_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR6259260.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(4328..4579)
                     /locus_tag="OO468_002150"
     CDS             complement(4328..4579)
                     /locus_tag="OO468_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR6259261.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(4694..4780)
                     /gene="azuC"
                     /locus_tag="OO468_002151"
     CDS             complement(4694..4780)
                     /gene="azuC"
                     /locus_tag="OO468_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR6259262.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            5043..5366
                     /locus_tag="OO468_002152"
     CDS             5043..5366
                     /locus_tag="OO468_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR6259263.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            5537..6034
                     /gene="ftnA"
                     /locus_tag="OO468_002153"
     CDS             5537..6034
                     /gene="ftnA"
                     /locus_tag="OO468_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR6259264.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(6071..6310)
                     /locus_tag="OO468_002154"
     CDS             complement(6071..6310)
                     /locus_tag="OO468_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR6259265.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            6500..7711
                     /gene="tyrP"
                     /locus_tag="OO468_002155"
     CDS             6500..7711
                     /gene="tyrP"
                     /locus_tag="OO468_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR6259266.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(7773..8438)
                     /gene="yecA"
                     /locus_tag="OO468_002156"
     CDS             complement(7773..8438)
                     /gene="yecA"
                     /locus_tag="OO468_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR6259267.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(8634..8720)
                     /locus_tag="OO468_002157"
     tRNA            complement(8634..8720)
                     /locus_tag="OO468_002157"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8684..8686),aa:Leu,seq:taa)
     gene            complement(8733..8806)
                     /locus_tag="OO468_002158"
     tRNA            complement(8733..8806)
                     /locus_tag="OO468_002158"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8772..8774),aa:Cys,seq:gca)
     gene            complement(8861..8936)
                     /locus_tag="OO468_002159"
     tRNA            complement(8861..8936)
                     /locus_tag="OO468_002159"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8901..8903),aa:Gly,seq:gcc)
     gene            complement(9088..9636)
                     /gene="pgsA"
                     /locus_tag="OO468_002160"
     CDS             complement(9088..9636)
                     /gene="pgsA"
                     /locus_tag="OO468_002160"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR6259268.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(9693..11525)
                     /gene="uvrC"
                     /locus_tag="OO468_002161"
     CDS             complement(9693..11525)
                     /gene="uvrC"
                     /locus_tag="OO468_002161"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR6259269.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(11522..12178)
                     /gene="uvrY"
                     /locus_tag="OO468_002162"
     CDS             complement(11522..12178)
                     /gene="uvrY"
                     /locus_tag="OO468_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR6259270.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            12474..12650
                     /locus_tag="OO468_002163"
     CDS             12474..12650
                     /locus_tag="OO468_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259271.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            12637..12861
                     /locus_tag="OO468_002164"
     CDS             12637..12861
                     /locus_tag="OO468_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR6259272.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(12894..13514)
                     /gene="sdiA"
                     /locus_tag="OO468_002165"
                     /pseudo
     CDS             complement(12894..13514)
                     /gene="sdiA"
                     /locus_tag="OO468_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(13744..14496)
                     /gene="yecC"
                     /locus_tag="OO468_002166"
     CDS             complement(13744..14496)
                     /gene="yecC"
                     /locus_tag="OO468_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR6259273.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(14493..15161)
                     /gene="tcyL"
                     /locus_tag="OO468_002167"
     CDS             complement(14493..15161)
                     /gene="tcyL"
                     /locus_tag="OO468_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR6259274.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(15176..16162)
                     /gene="dcyD"
                     /locus_tag="OO468_002168"
     CDS             complement(15176..16162)
                     /gene="dcyD"
                     /locus_tag="OO468_002168"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR6259275.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(16267..17067)
                     /gene="tcyJ"
                     /locus_tag="OO468_002169"
     CDS             complement(16267..17067)
                     /gene="tcyJ"
                     /locus_tag="OO468_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6259276.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(17155..17706)
                     /gene="fliZ"
                     /locus_tag="OO468_002170"
     CDS             complement(17155..17706)
                     /gene="fliZ"
                     /locus_tag="OO468_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR6259277.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(17752..18471)
                     /gene="fliA"
                     /locus_tag="OO468_002171"
                     /pseudo
     CDS             complement(17752..18471)
                     /gene="fliA"
                     /locus_tag="OO468_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(18636..20288)
                     /gene="fliC"
                     /locus_tag="OO468_002172"
     CDS             complement(18636..20288)
                     /gene="fliC"
                     /locus_tag="OO468_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR6259278.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            20554..21960
                     /gene="fliD"
                     /locus_tag="OO468_002173"
     CDS             20554..21960
                     /gene="fliD"
                     /locus_tag="OO468_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR6259279.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            21985..22395
                     /gene="fliS"
                     /locus_tag="OO468_002174"
     CDS             21985..22395
                     /gene="fliS"
                     /locus_tag="OO468_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR6259280.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            22395..22760
                     /gene="fliT"
                     /locus_tag="OO468_002175"
     CDS             22395..22760
                     /gene="fliT"
                     /locus_tag="OO468_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR6259281.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            22838..24325
                     /gene="amyA"
                     /locus_tag="OO468_002176"
     CDS             22838..24325
                     /gene="amyA"
                     /locus_tag="OO468_002176"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6259282.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(24359..24772)
                     /locus_tag="OO468_002177"
     CDS             complement(24359..24772)
                     /locus_tag="OO468_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6259283.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            24959..26164
                     /gene="yedE"
                     /locus_tag="OO468_002178"
     CDS             24959..26164
                     /gene="yedE"
                     /locus_tag="OO468_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR6259284.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            26161..26394
                     /gene="yedF"
                     /locus_tag="OO468_002179"
     CDS             26161..26394
                     /gene="yedF"
                     /locus_tag="OO468_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR6259285.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            26501..27171
                     /locus_tag="OO468_002180"
                     /pseudo
     CDS             26501..27171
                     /locus_tag="OO468_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(27211..>27457)
                     /locus_tag="OO468_002181"
     CDS             complement(27211..>27457)
                     /locus_tag="OO468_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6259286.1"
                     /translation="VLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVN
                     GARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         6825 a         7069 c         6537 g         7026 t
ORIGIN      
        1 attgaccagg ccacagagaa cgcccagcaa caaacccgct gccacgccaa tccacaggct
       61 ttcagtcagg ttgataacca ccgccgtggt gacacccgca caggcaatta cggaggcgac
      121 agaaaggtca aagtcaccgg aagcaaggca gaacaacatg ccacacgcca ccatccccga
      181 catggaaatt gccaggccca accctttcat attaatgaag gtggcaaaat ttgggacaaa
      241 aatggcacag gcgataaaga gcaccgcaaa caccaccagc atgccgtact gatcccagat
      301 acgcccgaag ctgaatgacg acttaggtgc gccagacccc gatgtagaaa cagaagacat
      361 catactctcc ttactcaggc aactgcctgg ctgactttag gcatcgcaag gctcagtgcc
      421 tgacgctcat ctgcctgctc gtgtaacaat tcaccggcga tttcgccttc ccgcatcacc
      481 acaatccggt cggcaacgcc gaggacttca ggtaagtcgc tggaggcaaa cagcaccgcc
      541 acgccctgcg ccgccagcgc ataaattacg ttatatattt cgtgcttagc gccaacatca
      601 atgccgcgcg taggttcatc cagcaaaatg accttcatct cttccgataa ccagcggccc
      661 agaatggctt tttgctgatt tccgcctgag agattcatga tcagttgctc cgcgcccggc
      721 gttttgatgt tgagcgaacg aatgtgctga tcggcattgt tttcttccca actgttgttg
      781 attacacaac cgccgagcac atgtttgcgt ctggcactga tgttgatatt gtcgcgaacg
      841 gagtgcacgg gaataatgcc ttccgctttg cgagcttccg ggcagagcat catgcctgcg
      901 gcaatggcgt ggctcggttt acgaatatcg atcggctgtt ggtcgatata aacctgaccg
      961 gcggtgattt gcgtcccgcc aaacaagcct ttcattaatt cgctacgccc cgcccctacc
     1021 agaccaaaca gcccaacaat ttcaccactg cgaaccgcca gacttattgg cgtacgcacg
     1081 cctagtgctt tcacagcatc aagacgcagg cgctcctcgc cataactacg aggttgccag
     1141 ccgtagatat cgccaatgtc gcgcccgacc atcgcctgca ccagcgcgtc gtggtcaacc
     1201 tgctgcatat cggtaaaggt tttgacataa cgtccatctt taaagacagt gatggcatcg
     1261 ctgagggcaa atatttcttc catacggtga gaaacgtata agatcacccg cccctctttt
     1321 cgcagttcac gaataacgcg gaaaagattg ccgatttcgc gggcagaaag ggagctggtt
     1381 ggctcatcaa aggcgataat tttagcgtta cgcgccagcg ctttggcgat ttcaaccatc
     1441 tgccactgac caatggcgag atatttcagc ggcgtgtccg ggtcaatatc cataccaaga
     1501 tgtttaagtt gtaaacccgc ctcataattc agcaatgagc gattcacaat gccgccttta
     1561 tgcggcagct ggccgagata gatgttttcc gcgacggtca tttccggcac gagatgcagt
     1621 tcctggtaaa taatcgccac acccgcgtta agtgctgcgg tcgtgtcgga aaaggacatt
     1681 tcctgcccat taatcactac agaacccgtg gttggcgcat agttgccgct gaggattttt
     1741 aagagagttg attttcctgc gccattttca cccatcaacg catgaacctg accggcatag
     1801 cagtcaaaac taatatccgt cagcgcctta acgccgggaa acgttttacc gatgccgcga
     1861 aatgagagat acggggtaga ctgttgcata acgtctccgt gaatcactgg tcgttcacac
     1921 cccctctgca tcatgcagag gggaattttt ccggcaaatt acttaccgcc taaacctttt
     1981 ttctccagtt cttctttaaa gttgtcacgc gtgatcagta ccacgtctgt aacttcggta
     2041 aattttggcg tttcaacgtc ttttgctacc cagttgtaaa gcatttcgct ggatttatag
     2101 ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc cggttgcctg tgctttagac
     2161 agttcgctta ctgcatccac accgttaata ccaataccga tgatatcggc tgctttaaag
     2221 ccctgacctt cggttgcacg tacgccgcca agtacggtgc tgtcgttcat accgacgatc
     2281 agccaatgtt taacttccgg atgttgaacc agcattgagt tggcagcgtc aaatgccccc
     2341 gggatgtcgt tagatttggt aggtacctga taaatttgtt tttccgggaa tcctgcagct
     2401 ttcagtgcgt ccatagaacc cgttgtacgg cggcgggcgg tatccagttc gttggcggta
     2461 atcgccatca ctgcgctttc tttgacatcc cagccacgtt tctgcatctc tttatacagt
     2521 tcctagccct gacgttcgcc aattttagtc gccgccatca tcaccagagg aacggtatcc
     2581 attggcttac ctttggcgtt aacaaactgg tcatccactg caatgacttt catatcgtag
     2641 ccacgcgctt tcgcgacgat ggcagagccg agtttcgggt ccggagtaca aataacgaaa
     2701 ccttttgcgc cactggctgc caggctgtcg atcgcgttca atgttttttc gccatccggc
     2761 acggcaatct taataacctc aaaccctaaa tccttcccgg ctttatcggc aaacttccat
     2821 tcggtctgga accacggctc ttccggttgc ttcaccagaa aaccgagctt caggttctcc
     2881 gccatagcgg attgtgacat aacggctgcc agaccaatgg ctgccagggc tttagtaaat
     2941 ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta gggcaaaaac gaatgacatt
     3001 cgttaaatta atcggaaaac aaagcattac cttttatcta aaagataagt gaccgtgttg
     3061 acatagtttt agcgagaaat taattctcca taggagagca atatcacatc gcagaattac
     3121 agtgagaacg tgcataaatt tagcgggaaa agacataagg aaaagccaat ttgtcagaca
     3181 aattgttgaa tgcacagcag attaatccat aagattagcc tggaaatcct tgttgtcttc
     3241 ggtacccatg cgggatgtct tctttttaac cagtcaatag gccgcattac ctggcgttga
     3301 gtttttgaaa tggtgtaata accacaactc aaagatgtgg aaaatgcgcg tcattcattt
     3361 cgccattaat tatcactgtg ctcattaatt aacagaacac gtataatgag agccatctcg
     3421 caaaaatgaa aaaacgtttt ataaaatcat cacttcatca tgaattcaaa ttcatcgatt
     3481 aatatcaaca agatacaaaa agcactatca ttaaaattca ttgcatttac attgatttca
     3541 ccaatgaaat gtaaaaatat ataaacttga tgatttaagc attttcttat aaccgttcag
     3601 acgttattct tatttcagat catcgtcaga attgactcca cgatcacatt tcggaccggc
     3661 agaaaggaat tattctgcaa acagtaatta tggtgttttg atttatcttg cacctctcca
     3721 cttctggata taaggatatt aggtatggca accgctggaa tgcttctcaa actcaactct
     3781 caaatgaacc gcgagtttta cgcatccaat ctctaccttc acctgagtaa ctggtgttct
     3841 gaacagagtc tgaacggcac cgccactttc cttcgcgccc aggcacagag taatgtgacc
     3901 caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg ctacccccat cgttaaagcc
     3961 attgatgttc caggtgaaaa actgaactct ctggaagaac tgttccaaaa aacgatggaa
     4021 gaatacgagc aacgttcaag cactctggcg cagttagccg atgaagcgaa agaactgaat
     4081 gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag aacagcagca tgatggcctg
     4141 ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac ttgcgggtat gtgccctgtg
     4201 cagaccgacc aacatgttct gaatgtcgtg tcacaccagc tgcattgatc atacatcggc
     4261 gctaatacat tgcgccgatg aagtttttga gaaaccgctg cctcatctgt ttgaagcagc
     4321 ggttttttta atgggattca ccctgtgggg taaacttgag ttcaatcagc gcgatggctt
     4381 tttggattgc ccgcatggtg accgggtctg cggcagcggg atgatgagta aagtcgatat
     4441 tcttcagctg actggacatt ttttcacgaa cttcaacggg cgcgattaca tcgagaacat
     4501 ccagaatttg tttgataacc aactggcaag ctaccacatc agaaaccagt tcctgatcgg
     4561 cattcagcgg ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg
     4621 gcttcacatc ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg
     4681 agcaggtttt ttgttatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat
     4741 agttattgaa catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg
     4801 ttatacaggt gttattgtct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt
     4861 tatgtgatac aaatcacata aatacccctt taatgttata aaaatgataa tcaaaaaaca
     4921 gcccccctat ttctgacacc tacagatggc aagaaatagc gcctgccagg cgtcttttcc
     4981 ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga
     5041 ccatgagact tctgattctg actctttcat taataacgct ggcggggtgt acggttactc
     5101 gccaggccca tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc
     5161 aggctttttt gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg
     5221 catgccagca ggaaggctat acccacgcga tcccctttgg tcagccagta ggcaactgta
     5281 gcctttttgc gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc
     5341 attccgcctt ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta
     5401 aagaaatagc ggtaatgcgt tttattccca ttcgtatttt taataattga agtttatatt
     5461 ttaccttttg caaataataa aataacaaat tatagtgacg ccacagcaac aaatataacc
     5521 tttgtggagc actatcatgc tgaaaccaga aatgattgaa aaacttaatg agcagatgaa
     5581 cctggaactg tactcttcac tgctttatca gcaaatgagc gcctggtgca gctatcatac
     5641 cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag gaagagatga cgcatatgca
     5701 gcgtctgttt gattacctga ctgataccgg caatttaccg cgtattaata ccgttgaatc
     5761 tccgtttgct gaatattcct cacttgatga attattccag gaaacctata aacacgaaca
     5821 attaatcacc cagaaaatta acgaactggc tcatgctgca atgaccaatc aggactaccc
     5881 aacatttaat ttcctgcagt ggtatgtttc tgagcagcat gaagaagaga aactgttcaa
     5941 atcgattatt gataaattaa gcctggcagg caaaagcggc gaaggtctgt attttatcga
     6001 caaagaactc tctaccctcg acacacaaaa ctaatgctct ccggcggcag gtttctgccg
     6061 ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt cgaaaaacct tcttctgaag
     6121 gtataaattt gccttttgct gccagaaacg ctaccagctc tcccgctgtc accccttctg
     6181 ccgagcacgt gtgaaaacgt gcctgttcac caaaacgcgc tttaatcgca gcttccagac
     6241 tggcatgcgt atattgctcg cctgattcaa tcatcatatt taacacttca tgaccgtgaa
     6301 tagagtccat cgttcctcct caaaaaaagc ctagcgtagc gattaccgct tatgaagact
     6361 ttgcgccagc gcaggactga atgcttttat tgtacattta tatttacaca atatgtaacg
     6421 tcggtttgac gaagcagccg ttatgcctta acctgcgccg cagatatcac tcataaagat
     6481 cgtcaggaca gaagaaagcg tgaaaaacag aaccctggga agtgttttta tcgtggcggg
     6541 aaccacaatt ggcgcaggca tgctggcaat gccgctggct gcggccggtg ttggttttag
     6601 cgttacgtta atcttgttga ttgggctttg ggcgttgatg tgctacacgg cgctattact
     6661 gctggaggtg taccagcatg ttccggcaga taccggtctg ggcacgctgg caaaacgcta
     6721 tctgggacgc tacggtcaat ggctgacggg cttcagtatg atgttcttaa tgtatgctct
     6781 gactgcagca tacatcagcg gtgccggtga attgttggcc tccagcatca gcgactggac
     6841 aggtatttca atgtcggcaa ccgctggcgt gctgttgttc acttttgttg ccggtggcgt
     6901 ggtttgtgtt ggaacatcgc tggtcgattt atttaaccgt tttctgttca gcgccaaaat
     6961 tatttttctg gtggtgatgc tggtattgct gctaccgcat attcacaaag tgaatctttt
     7021 aaccctgccg ttgcaacagg ggctggcact gtctgcaatc ccggtgattt ttacgtcgtt
     7081 tggttttcac ggtagcgtgc cgagtattgt cagctatatg gatggcaaca ttcgtaagct
     7141 acgctgggtg tttataatcg gtagtgccat ccccctggtg gcatatattt tctggcaggt
     7201 ggcgacgctt ggcagcattg attcaacaac ctttatggga ttgctggcta atcatgctgg
     7261 attaaacggg ctgttacagg cgttacgcga aatggtggcc tctccgcatg ttgagctggc
     7321 ggtgcattta tttgctgatt tagccctcgc cacgtcattt ctcggcgttg cgttaggctt
     7381 atttgattat ctggctgatt tgtttcagcg ttcaaatacc gttggtggac gattgcaaac
     7441 tggtgcaatt acctttctgc cgccgttggc gtttgcactg ttttatccac gaggatttgt
     7501 gatggcgctg ggttacgccg gtgtggcgct ggcggtactg gcattgatta tcccttcact
     7561 attgacctgg caaagcagaa agcacaatcc tcaggcgggt taccgggtca aaggtggtcg
     7621 tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg attggcgtgc aatttttgat
     7681 tgcggcaggg ttgttaccag aagtggggtg atcagatagc ctcaaattcc ttattgggtg
     7741 ccagaattaa cgctgacacc caatgtgacc tcttaatgca ggcagcactg cttaaatttc
     7801 ttaccactgc cgcacggaca aggatcgtta cgccccggtt tctcttctgc tttgatcggt
     7861 tgctgaacag ctttttcctg cggatgcgcc atccagtatg catgtaaatc aagcgccgcc
     7921 agtcgaatgg catctacgct ctcttcaaac gcttctggcg acattttctc tacccgctcg
     7981 aagttttcct cagtaccgtg cagcgcaatc gcctccagcg ctggttttaa agaatcgggc
     8041 aacgttgacc agtcagaaag tgccacgccc cgcatatagc caaagcacca ctcctcaaca
     8101 atcgtcagct cgctgccatc aacttctcgc aagccgaata acggctcaaa ctgctccggg
     8161 aattcgttca gacgctctgc ggtatcggcc atatgttgaa aagccaggtt cataaagcgc
     8221 gtcatctctt tctctgacgc ccaacgcggc acatagtcag ccccacccca cacggcaacc
     8281 agccactgtt ctggttcaat ctcttgcgga gaactcaaca ccgccgtcaa taaaccgtcc
     8341 agctccgcca catcaaggat ggcgtggtca gtgttgtatt tggtcagaat atcgtccagc
     8401 cattccaact cactttcgtt taacggtccc gttttcatac gcttttcctt gtggatctca
     8461 actcgccagc acctatctta catgccggtc cgtatcagag atactttttg agtggctttg
     8521 ccggtgatta aaaattaagg agggtgtaac gacaagttgc aggcacaaaa aaaccacccg
     8581 aaggtggttt cacgacactg cttattactt tgattttatt cttatctttc ccatggtacc
     8641 cggagcggga cttgaacccg cacagcgcga acgccgaggg attttaaatc ccttgtgtct
     8701 accgattcca ccatccgggc tcgggaagaa agtggaggcg cgttccggag tcgaaccgga
     8761 ctagacggat ttgcaatccg ctacataacc gctttgttaa cgcgccaaat tcttcaggcc
     8821 tttcagccag acatccgctt gacgccgatg tcttttaaac tggagcggga aacgagactc
     8881 gaactcgaga cctcgacctt ggcaaggtcg tgctctacca actgagctat tcccgcattc
     8941 atcaagcaat cagttaatca cttgatttta ttatcgtctg gcaatcagtg ccgccgttcg
     9001 atgcgttgca ttctacttac ctggcgcgat gagtcaacga tatttttcac cacttttgat
     9061 cgtttgctga aaattacgcc gaaacgatca ctgatcaagc aaatctgcac gcgcagcgct
     9121 caaatattgc aacattgacc acagagtcag taccgcagcc acaaagaaaa gtgcaatacc
     9181 gacgtactca acccaaatgt tcggacgcca cagcagccag gccaacgcca ccatctgggc
     9241 agtggttttc actttcccaa tccaggagac ggccacgcta ctgcgtttac ccaactccgc
     9301 catccattcg cgtagcgcag aaataataat ttcacgggcg atcatcgttg ccgccggtaa
     9361 tgtcacccac cagctgtgat aatgctcggt taccagcacc atggcgatag ccacgagaac
     9421 tttatctgcc acagggtcaa ggaaagcacc aaaccgggta ctctggttcc agcggcgtgc
     9481 cagaaaacca tcgaaccagt cagtcaccgc cgcgacgcag aaaatgagcg cggcggcaaa
     9541 cggcgaccag gtgacaggca gataaaagac caatacaaag aatgggataa ggatgacacg
     9601 gaacagtgta agcaacgtag ggatattaaa ttgcataatg acgggtaact atctgttgtc
     9661 agtaagatta cccctatgtt gctacagaga catcaatgtt tcaacgacca gaagatcttt
     9721 tctgccagac cttgcgaaat acccggcact tttgcaattt cctcgacgct ggcgttacgt
     9781 aaaccttgca aaccgcccat atatttcaac aacatttgcc gacgttttgg cccgacgcct
     9841 tcaatggttt ccagggaact ggtatttttg accttcgccc gttttttacg gtgcccgcca
     9901 atcgcgtgat catgtgattc atcgcgaata tgctggataa catgcagcgc gggtgaatct
     9961 ggcggcaaac taaatccctc accttccggc tcaaagaaca gcgtttccag tccagcctta
    10021 cgatctgctc ctttggcaac gccaagtagc agcggatgat ttttatccca tgagacatcc
    10081 agttcggcga agacattttt cgcctgcgca agctggcctt tgccgccgtc gataaggatc
    10141 acatccggga tcttactgtc gtcaatggct ttaccataac gccgacgcag cacctgattc
    10201 atcgccgcat aatcatcgcc cggcgtgatg cctgtaatgt tatagcgccg atactccgca
    10261 cgcagcgggc cgttagcatc aaacaccaca caggaagcga cggtttgttc gcccatggta
    10321 tggctgatgt caaagcactc catccgcttc acttccggca atttcaacac gctggcaagc
    10381 gcggtcagtc gctggtgaac ggtagattgc tgcgaaagtt tgctggttaa ggccgtcgcc
    10441 gcattggtgc gcgcgagttt cagataacgc gccctatcgc cgcgaggttt ggtttgaaca
    10501 ttaatcttgc gtcccgccag ttctgaaagg gaatcggcga gcagcgtttt atcgctaaga
    10561 ttaaaatcga gcaggatctc acccggtaag gtgcgcatct ggctgccttg taaatagaac
    10621 tgacctacga aggtttctac cacctcgctc agttccgtac cgccaggcac tttcgggaaa
    10681 tagctgcggc tgccgagcac tttgccctga cgaatgaaca atacgtggac acaagccatg
    10741 cccgcatcga acgccacacc aataacgtca aggtcgtcgc cggtattgga aacaaactgt
    10801 ttttcggtga cgcgtcgcac cgcctgaatt tggtcgcgaa tacatgccgc ttcttcaaac
    10861 tccagattct ggctggcagt ttccatacgg ctaatgagtt gcgtaagcac ctgatcatct
    10921 ttgccagaca aaaacaggcg cacatactcg acctgctgag cgtattcttc ttcactcacc
    10981 agtccttcaa cgcacggtcc cagacagcgc cctatctggt attgcagaca cggacgcgag
    11041 cgattgcgat aaacactatt ttcgcactgg cgaatgggga aaatcttttg cagtagcgcc
    11101 agtgtttcac gtacggcata gccattcggg aacgggccga aatattcacc tttggcatgc
    11161 tttgcaccac gatgcatcgc cagacgcggg tgggtatcgc cactcaggaa gataaaagga
    11221 taagatttat catcgcgtag caaaacgttg taacgcggct gatagagttt gatgtagttg
    11281 tgttccagca acagcgcttc ggtttctgta tgcgtaaccg ttacatcaat ttgctggatc
    11341 tgggcgacca gcgcttcggt tttgcgcgaa gcgaggttgc tgcggaaata gctggaaagc
    11401 cgttttttca ggtctttcgc tttgccgaca tagataaccg taccaccagc atcgtacatg
    11461 cgataaacgc ctggctggct ggttacggtt tttaaaaacg cttttgcgtc aaactgatca
    11521 ctcactgact tgataatgtc tccgcattac acagaccatg gcgaattgcc aggtgagtca
    11581 gctcaacatc gccatgaatg tttagtttac tgaacatacg atagcggtag ctgttcaccg
    11641 ttttcggact gagattgagc tgttctgaga tctcattgac cttctggccc ttagtgatca
    11701 tcagcataat ctgcaattca cgttcagaca aactggcaaa tgggctttct gttttttctg
    11761 gttcgatctg gcttaacgcc atttgttgag cgatatcaga agcaatgtaa cgctgccctg
    11821 aatagacaga acgaatcgca ctcacgactt cctgcggagc cgcgcctttg ctgaggtagc
    11881 cagcagcacc tgcctgcatg actttcgctg gtaaagggtt ttctgtatgg accgtaagca
    11941 tgatgatttt aacatcagct gtggaacgcg cgattttacg cgtcgcctca agaccgccaa
    12001 tgcccggcat actcatgtcc attagcacca cgtcaacggc atttgtccgg caccacttaa
    12061 cggcgtcttc accgcacgat gcctctccga cgacttttat acccttaata tcttccagaa
    12121 tgcgtcgtat ccctgcgcgc accagttcgt ggtcatcaac aagtagaacg ttgatcaaag
    12181 gaatatctcc agaaataggg ataacgctac tgatagttag tcattcgtat attagcggtt
    12241 tttattgcaa ctttgaaacg ttaaaaatgg tgggtattcg atttttctct cgtttttgtc
    12301 aattcgattg tccacagtta tggtcacgcc cgtcaccaac ggcttaccgg atcgtttcac
    12361 agtaagccct acgatgaaat gtgacaaaaa ttgactttat tcagcaaaaa tgaaaatcag
    12421 cccgctgaat atgtaacatt aattaacccg aacaatacgg gcaaaaaaca ttcatgataa
    12481 aaatatttat tggtcattat attaacgtat tttatagcac tgccgatatc acgctcaaaa
    12541 aacaaccact gctattttta gcaaagctta tggtatactc cgccgcctta acatttttca
    12601 ccacaaattt tcattgcaac atgacgagga aaataaatga gtacgcctga tttttctact
    12661 gccgagaata atcaagaact ggcaaatgaa gtctcctgcc tgaaagcgat gctgacgctg
    12721 atgctgcagg cgatgggaca agctgacgcg ggccgcgtga tgttaaagat ggaaaaacag
    12781 cttgcgctga tcgaagacga aacccaggct gcagtatttt ccaaaacggt taagcaaatt
    12841 aaacaagcct accgtcagta atataaaacc ggctgagagc gtgcctttca gccggttatt
    12901 gacaaaatca tcgctatctc tgctgatgtc ttcccttccg ccgtccattt cagaatttct
    12961 ttttcgcgct tgctgaaatt catctctggc gtcatcacta tttcatcatt taaacgcatc
    13021 agagccatca gactttcgcg caccagtaac tgcattttta attgcagttc atcactaaga
    13081 atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa agcccagcgc ccgatttggc
    13141 agcattaaat actgagtgac accgcggcgt aaaccatgtg cgtgcgcggc ttcccataat
    13201 ggctgtgctt cgctgaataa gtcatcattc cacattaaat ggccctgact aaagttttca
    13261 gggttcagca caggatcaat tgcgagaaaa ttttttgcct gataataact aacccacgac
    13321 tcagggtaat tggtgtagaa agccacttta ggtcgcgtga atggtaccgg gtggcgaaca
    13381 cataacgaat agtaatcgta ctccagctgc tgagcctgaa gctctatttc atggtaggcc
    13441 tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg tgcgacgcca gctgaaaaaa
    13501 tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg agtatcattt ataatgataa
    13561 ttcaaactat cacataaata agatttatat ataatttata ttattcaggc aatgaattac
    13621 ttttgcaagc catcgcattc tcttatgtta ttaatgagtt atgctgattt gttaagcagt
    13681 tttatcacgc ttgaaatggc gtcccgcccc gacaggtgaa tcgtcggggc tgattttttc
    13741 ttattattgc agcagaagct tctcgaggaa ctggcgggtg cgaggctgct ggggatcggc
    13801 aaataacgct tttgctgccc cctgctcgac tatccgcccc tggtccataa agatcgcccg
    13861 gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca atcaccatcg tgcgcttttc
    13921 ctgcgccagc tggcggatgg tgttcaggac ttcgcccacc agctctggat ccagcgccga
    13981 cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc agtgcacgcg caatcgcaac
    14041 acgctgctgt tgaccgccag acaaacgacg tggatagctg gtttctttac cggccagccc
    14101 aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct tctttcggtt cgcctttgac
    14161 gatcaccggc ccttcaataa tgttttccag caccgtacga tgcggaaaca aattaaagtt
    14221 ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc agcgattttt gctgacttaa
    14281 tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc gttcccgctt ccggttgttc
    14341 cagcagattt atgctacgta gcaatgtggt tttgccggaa ccactcggac cgataattgc
    14401 caccacttcg ccaggcttta cctcgaggtc gataccgtgc agcaccgtct gaccgtggaa
    14461 ttttttcacc aggttcttaa cttcaatggc actcattttg gttctctctc ctggcgatta
    14521 agttgattct caaaatggtt ctgcaacgtc gataacacag tcgccatgat ccagtagatg
    14581 agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc gcgaggtaat caactgcgcc
    14641 tgacggaaca gctccggcac ctggattgtc gcggccagcg acgtatcttt taccagactg
    14701 ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg cctgtggcaa aatcgcgcga
    14761 cgcatggtct gccacggcgt cataccaata ctggcagcgg cttcccactg acctttatca
    14821 atagaagaaa tggcggcgcg cagcgtttcg gcggcatagg cggcagtgtt cagcgacaga
    14881 ccaatcattg ctgacggaat gggatctaat tcgataccaa actgcggcag gccgtaatag
    14941 atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa tagagatata aaaacgcgcc
    15001 agccagcgca cgggccagat tggcgacagg cgcatcagcg cgagaataaa cccgagtagt
    15061 aagccaaaaa acatgccgcc gatactgagt tgcagggtat acccagcccc tttcaacagg
    15121 aacggcaagg aatcaataac cagttgtata ctttcttgca tgagcgttcc cggagctaaa
    15181 cgtggggatg ataggcgaac agcgcaggcg cgccgccggt atgaataaac agaatcggcc
    15241 cttcatcttt gaagcgtttc tgactgatac cgtcaatcag ccccgccatc gcttttccgg
    15301 tatacacagg atcaagcaga atgccttcca gacgcgccag caatttcact gcttccatgc
    15361 cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc atcccagagt aaaatttccg
    15421 ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg taggttaacc actttcggca
    15481 attgatcggc aacggaacgc gacacggtca cgccaatcag ttcgctttca ggcatcaggt
    15541 gttccagccc aacagccagt ccggcgtgag ttccggcact gcccgatgcg actaccaccg
    15601 acgaaatatt aaccgcccct tcacactgtt gcgcgatttc cagcgcactc tccacataac
    15661 ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac atacgggcga aagccttgtg
    15721 cttcgactcg cgtcgccagt tcttccagtt gggcattggg atcggtcagt gcgtcgcaca
    15781 tttcaatctg ggtattgaac agatccagca acaaacgatt gccgttggtt aaatagtttt
    15841 ctgcggttgt gccaatagga ttttccagca gcgccacgca gtgcagaccg agtttcgccg
    15901 cgactgcggc agtctggcgc acatggttag actggatcgc gccggcagta atcagcgtat
    15961 cggcaccttc gcgcagggca tctgccgcga gaaattccag cttacgtaat ttattgccgc
    16021 ccattgccat gggggtgacc tcatcccgtt tgatgaaaat ttcccgtcca agataatcag
    16081 aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat aaactccaac cgtggaaaac
    16141 gggttaaatt atgcagtggc ataacagcct ccgatgtgtg ttgttgtgat tttcttatta
    16201 tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa agcgcccttt tttgtcatta
    16261 tgctgattat ttggtcacat cagcaccaaa ccatttttcg gaaagagctt gcagagtgcc
    16321 gtctttttgc atttccgcaa ttgcatcatt caccgctttc agcaggtctt catttccttt
    16381 acgcagcgcc acgccagact cctgacggga gaatgcttca ccggttactg ccagcgtatc
    16441 gttggttttc ttcaccagat ccagcgccgc cagacgatca acgaggatcg catcgatacg
    16501 ccctacgcgc agatcctgat atttggtcgg gtcatcatca taggtacgca catcgacacc
    16561 ctgaacattc tgccgcagcc actcttcata gttggtgccc agaccgacac ccactttttt
    16621 gcctttcaga tcagcagccg ttttaatggt gccttcgtta ccttttttca ccagcgcctg
    16681 aataccggaa atggtgtaag gggtagagaa atcgtatttt ttcttgcgct cgtcagaaat
    16741 ggtgacctga ttaatcacca catcaatgcg tttagagtcc agcgacgcca gcataccgtc
    16801 ccatttggtc ggttttagtg acgcctcaac gccaagatgt tttgccagct gttgggcaaa
    16861 ttccacttca aaaccggtta atttgccgtc atccccctga aaactgaacg gcggataagt
    16921 gccttccagc cctaccagca gcgtgccgct ctctttgact ttattaagca gaccttcatc
    16981 tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg gccatcacac ccatcaatgc
    17041 ctgacgtccc agatgtgcta atttcatatt caccccgaat gttgttatgt ctgtttgcag
    17101 tgtagagcca ttgtttgtaa acacaaaaac aactccgcta catcttattc ttatttaata
    17161 tatatcagaa gcaggcaggc tggaggattt ctgcacaagt ccggtacagg tttggcgctg
    17221 atagtgttga tacttccgta atgcgatgcg gtaattgttg gtgctggtgg tgggcagcca
    17281 cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga tcgagggaaa tattttgctc
    17341 atgtagatga ttacccaaac ggcgcagacg aacgacatat tcacgcaccg tacctgggct
    17401 catttcagtt tgctcaaaaa gaaatttctt aaagccgata atatcgaaaa aatcactctg
    17461 cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc gcccatgatt gttgttccgt
    17521 ttgccagcct ttttcatcaa gcagcgtgtc caggcgggaa atctcgattt tattcactat
    17581 tttgccgtcg cgaactaagg taatgcgatc gagtaattta cggcaatgcg cgcaatgggt
    17641 ctggctgtgt ttaaagtctt taagatagcg gcttaatggc cgtcttttca ggtgctgcac
    17701 catcattaag aactcctggt agtcaaagtt aaagtgcggc atttactgac gttataactt
    17761 acccagttta gtgcgtaacc gtttaatgtc ctggctgtgt aactgactga cccgcgattc
    17821 cccgacctcc agcaccgcgc caatctcttt gagattcagc tcttcctggt aatagagggt
    17881 taataccagt ttttcgcgct ccggcaacgt ttcgatggct tccatcaccc gctggcgcag
    17941 attactgtcc agcagttgtt gtagcgggtt ttctcgctga tgatcatcag taaccagttc
    18001 gatgctatcg ccgtgctctt cacgccactc atcgtaggag aagagctggc tgttattggt
    18061 gtcgagcagc atttgacgat aatcggcaat atcgatccct aaacgttccg ctacctcagt
    18121 ttccgtggcg ttgcggccaa gttcctgctc cagttgccct attgcctgtg ccacttcacg
    18181 cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg cgaagttcat ccagcatagc
    18241 gccacggata cgctacactg cgtaagttgt aaatgccgtt ccttgtaggg cgtcatagcg
    18301 ttcgacagca ttaagtaacc caatgccgcc cgcctgtagc agatcgtcaa gttccacgct
    18361 cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc accagcggga cataacgctg
    18421 ccacagcgag tgtttatcca ttacaccttc agcggtatag agtgaattca cgataaacag
    18481 ccctgcgtta tatgagttat cggcatgatt atccgtttct gcagggtttt taatcggacg
    18541 attagtgggt gaaatgaggg gttatttggg ggttacgcaa aaagccgaat gtaaaaaacc
    18601 ccgccggtgg cggggtttga gaggcattca gacgattaac cctgcagcag agacagaacc
    18661 tgctgcggta cctggttggc ttttgccagc acggagttac cggcctgctg gataatctgc
    18721 gctttcgaca tgttggacac ttcggtcgca tagtcggcgt cctgaatacg ggactgcgct
    18781 tcagacaggt tggtagtggt gttgttcagg ttggtgactg cggaatccaa acggttttgc
    18841 accgcaccca gggaggagcg gaatttgtcg atggagctga tggcttcatc cagagctttc
    18901 aggggatcgg ctgtcgtagc tgcttttgtt tcagcatctg tcgtcaattt accatcagca
    18961 tctttataaa ctgccttacc gctgccgtta gtcactgaac catcatcctg taggtaaaaa
    19021 tctacagtat cagatgtggt caacgcctcg ttgttatcaa cataaaccga atcaacacct
    19081 tttgttacag cgccagttgt gccgttgacg ctataacctg cagtagtgaa atcaacattg
    19141 tttcccgagg ttgatacagc tttatcgaaa gctgcctgat caacttttgc acctgttact
    19201 gaataagtaa tagtgtccgg cgtagtacta ttaccggtaa aggagataga ggtaccgcca
    19261 atgtcaatgg ttgtcgcttt ggtattattg gctgcaagtg tggacagact agcctcagtc
    19321 aaagaagcac cagagccgtt tttcgttaag cgcccagttg tatcaatgta gagtttatct
    19381 ccattgcttg ccgtaatttt accatcactg ctgatctgaa catcctgtgc agaaccatcg
    19441 atagtaatag tgcccttagc ggtatcacca actcccgggg tcaaatattt ctgaacatcg
    19501 gcagctgaag ccgttgtggt atcaaaagag tatgacttac ttgcactgtc atacttgtaa
    19561 ttcgttgcac tagccgccgt tccgaagccg ttattaatgg ttgcctgaac agtatcacca
    19621 tcactaactc cagccagcaa atcagacgct ttagcagcat cgtaacccgc actcacagta
    19681 tatttgttgc cgaccactgt tgcattagca gctaccaagt cagctttaga tgcagcagta
    19741 ttagccacag ccccgccacc attcacatta aacccattca gccccagcgt atcagagtca
    19801 attttcttca gatcaatagt gatagtctgg ccgtcattcg caccaacctg aattttcatc
    19861 gaaccgtctt ttgccagtac gttcacgccg ttgaactggg tctgaccgga tacgcggtca
    19921 atttcgtcga gacgggattt gatttcgtcc tgaatggagt ccagatccga atcagagtta
    19981 gtcccggtag aagcctgaac cgtcagctca cggatacgct gtaagttgtt gttgatttcg
    20041 gacagcgcgc cttcagtggt ctgtgcaaca gaaataccgt cgttggcgtt acgtgcagcc
    20101 tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa tcgcctgacc cgcggcgtca
    20161 tccttcgcgc tgttaatacg caagccagaa gacagacgct cgatagaact cgacagcgca
    20221 gactggttct tgttgatatt attttgagtg atcagcgaga ggctgttggt attaatgact
    20281 tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt acgttttggg tttccacccg
    20341 tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa aacaaccttt agaatttttt
    20401 tcacaaacag ccattttttg ttagtcgacg aaatactctt ttctctgccc cttattcccg
    20461 ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc aggccgataa ccccggtatt
    20521 cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa gtatttcatc gctgggagtc
    20581 gggtcaggtc tggatttaag ttcaatcctt gatagcctca ccgccgcgca aaaagcgacg
    20641 ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta aacttagcgc ctacggtacg
    20701 ctgaaaagcg cgctgacgac tttccagacc gccaatactg cattgtctaa agccgatctt
    20761 ttttccgcca ccagcaccac cagcagcacc accgcgttca gtgccaccac cgcgggtaac
    20821 gccatcgccg ggaaatacac catcagcgtc acccatctgg cgcaggcgca aaccctgacc
    20881 acgcgcacca ccagagatga tacgaaaacg gcgatcgcca ccagcgacag caaactcacc
    20941 attcaacaag gcgacgacaa agatccgatt accattgata tcagcgcggc taactcatcg
    21001 ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg gcgtaagtgc gagcatcatt
    21061 aacgtgggta acggtgaata tcgtctgtca gtcacatcaa atgacaccgg ccttgataat
    21121 gcgatgacac tctcggtcag cggtgatgat gcgctacaaa gttttatggg ctatgacgcc
    21181 agtgccagca gcaacggtat ggaggtctcg gtcgccgccc agaatgcgca gctgacggtc
    21241 aacaacgtcg ccatcgagaa cagcagcaac actatcagcg acgcgctgga aaacatcact
    21301 ctgaacctga acgatgtcac cacgggcaac cagacgctaa ccatcactca ggacacctcc
    21361 aaagtgcaaa cggcgattaa agactgggtg aatgcctata actcgctaat agataccttc
    21421 agcagcctga ccaaatacac cgccgtagat gcgggagctg atagccagag ttctagcaat
    21481 ggcgcactgc tcggcgactc cacgctgcgg acgattcaga cgcagttgaa gtcgatgctg
    21541 agtaataccg tcagttcttc cagctataaa acgctggcgc agattggtat cacgaccgat
    21601 cccagcgatg gcaaactgga actggatgcc gacaaactca ccgctgcact gaaaaaagat
    21661 gccagcggcg taggtgcatt gattgttggc gatggtaaaa aaaccggcat cacaaccacc
    21721 atcggcagca acctgaccag ttggctttcg acaacgggca ttattaaagc cgctaccgat
    21781 ggcgttagta aaaccctgaa taagttaact aaagactaca acgccgccag cgatcgcatt
    21841 gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac tggacgtttt aatgacctcg
    21901 ttaaacagca ccagcagcta cttaacgcag cagttcgaaa acaacagtaa ttccaagtaa
    21961 tcattattca tcgggagaca ggtcatgtac gcggcaaaag gcacccaggc ctatgcacaa
    22021 attggcgtcg aaagcaccgt aatgagcgcc agccaacagc agttggtcac catgctattt
    22081 gatggagtgc tgagcgcact ggttagagcg cgactgttta tgcaggacaa caatcagcaa
    22141 ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg agaacggact gcgggtgagt
    22201 cttgatgaag agagcaaaga cgaattaacc caaaacttaa ttgctcttta tagctatatg
    22261 gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct cagcagtcga agaggtggaa
    22321 gcattaatgc gtaatattgc cgatgcctgg aaagagtctt tactctcccc ttcttcgatt
    22381 caggacccag tctgatgaac aatgcaccgc atttgtattt cgcctggcaa caactcgtcg
    22441 aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca atgggacgaa ctcatcgcta
    22501 gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca cctgactgaa gaggttgccc
    22561 cgtccactac gatgcaggag cagctccgcc cgatgctgca cctgattctc gacaacgaaa
    22621 gcaaggtaaa gcagttatta cagattcgga tggatgaact ggcgaaactg gtcggtcagt
    22681 catcggtgca aaaatcggtg ttaagtgcct atggcgatca gggcggcttt gtgctggctc
    22741 cgcaggataa cctcttttga atcttaatga gccgatggct cgcgaataat ccgattacgg
    22801 ctacgcttct aatattcccc ttgaatggag tcgaagaatg cgtaatccca cgctgttaca
    22861 atgttttcac tggtattacc cggaaggcgg taagctctgg cctgaactgg ccgagcgcgc
    22921 agacggtttt aatgatattg gtatcaatat ggtctggttg ccgcccgcct ataaaggcgc
    22981 atcgggcggc tattcggtcg gctacgactc ctatgattta tttgatctag gtgagtttga
    23041 tcagaaaggc agcatcccta ctaaatatgg tgataaagca caactgctgg ccgccattga
    23101 cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg gtagtcaacc acaaaatggg
    23161 cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat gctgatgacc gtacgcaaat
    23221 tgacgaagaa atcattgagt gtgaaggctg gacgcgttac accttccccg ctcgtgccgg
    23281 gcaatactcg cagtttatct gggatttcaa atgttttagc ggtatcgacc atatcgaaca
    23341 ccctgatgaa gatggcattt ttaaaattgt taacgactat accggagaag gctggaacga
    23401 tcaggttgat gatgaactcg gtaatttcga ttatctgatg ggtgagaata tcgattttcg
    23461 caatcatgcc gtgacggaag agattaaata ctgggcgcgc tgggtgatgg aacaaacgca
    23521 atgcgacggc tttcgtcttg atgcggtcaa acatattccg gcatggtttt ataaagagtg
    23581 gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt attgtggctg aatgctggtc
    23641 gcatgaagtc gataaactgc aaacgtatat tgatcaggtg gaaggcaaaa ccatgctgtt
    23701 tgatgcgccg ctgcagatga aattccatga agcatcgcgc atgggtcgcg actacgacat
    23761 gacgcagatc ttcactggga cgctggtgga agccgatcct tttcacgccg taaccctcgt
    23821 tgccaaccac gacacccaac cgttgcaagc cctcgaagca ccggtcgaac cgtggtttaa
    23881 accgctggcg tatgccttaa ttttgttgcg ggaaaatggc gttccttcgg tgttctatcc
    23941 ggacctctac ggtgcacatt acgaagatgt cggtggtgac gggcaaacct atccgataga
    24001 tatgccaata atcgaacagc ttgatgagtt aattctcgcc cgtcagcgtt tcgcccacgg
    24061 tgtacagaca ttatttttcg accatccgaa ctgcattgcc tttagccgca gtggcaccga
    24121 cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat gatggcgaaa aaaccattca
    24181 tctgggagag aattacggca ataaaacctg gcgtgatttt ctggggaacc ggcaagagag
    24241 cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc aacggcggca gcgtcagcgt
    24301 gtgggttatc gaagaggtga tttaaatcca tccccggcgg caagccgggg agatttcatt
    24361 acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc ggcacactct gccgtcgggc
    24421 gatccacacg ctccatcgtc ataccatcgt actcaatggt gttaccgtca cgctcaacct
    24481 cgtacagctc acgttttacg gtaacgttag tgagatcatc agacatcaat gtcaacttac
    24541 ccggcacggc aatcccccgc tgccactgac ggcaatccaa ggtatcgccc tctttagtga
    24601 caatcaggct gcctatcgct tccggactga ccagcgcacg ttgcggccct ttggtctgcc
    24661 agtagcccgc cagccagtcc ggcgcggggg ttttcacgac attgttatag ttttcgactt
    24721 cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc gagttttttc atcattcgtc
    24781 ctgcatgaga agaaaagagg attgtggcat taaagctctg atgtcgccag cctttccagg
    24841 ttcagagaga aatttgatct aactattatt tttgtgaatt attaccccga aaataaattt
    24901 gcatggtgta tgattcgcgg ctcttgtttc aaaccttctg agttcagagg ctacattcat
    24961 gtcatggcag caattcaaac acgcctggtt gattaaattc tgggcaccca tccctgcggt
    25021 catcgcggcg ggtattctct ccacttacta ttttggcatt actggcacct tttgggctgt
    25081 cacgggtgaa tttacccgtt ggggcggtca gctcctgcag ctgtttggcg tccatgctga
    25141 agagtggggt tattttaaaa ttatccatct ggaaggatcg ccattaaccc ggatcgacgg
    25201 catgatgatc ctcggtatgt ttggcggctg ctttgctgct gcgctgtggg ccaacaatgt
    25261 caaactacgc atgccacgca gccgtatccg cattatgcag gccatcattg gcggcattat
    25321 cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg gcggcgttct ttaccggcat
    25381 tcctcagttc tcgctgcatg cctggttctt tgccatcgcc actgccattg gttcatggtt
    25441 tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc gtgaaaatgc agaaagtttc
    25501 tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg cgtcgttttc gtctcgggat
    25561 gctggtcttt ttcggcatgc tgggctgggc gctgctcacg gcgatgaacc aaccaaaact
    25621 ggggctggca atgctgtttg gcgtcggctt tggtttactg attgaacgtg cgcaaatctg
    25681 ctttacttcg gcgttccgcg atatgtggat caccggacgt acccatatgg cgaaagcaat
    25741 cattatcggt atggcggtga gtgccatcgg gatcttcagt tacgtacagt taggcgttga
    25801 acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt ggtttacttt ttggttttgg
    25861 catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac cgcgcggtag aaggccaggt
    25921 gcactactgg tgggtcggtc tgggcaacgt gatcggctca acgattctgg cgtattactg
    25981 ggatgatttc gctccggcgc tggccaccga ctgggacaaa atcaacctgc tgaaaacttt
    26041 tggtccgatg ggaggcctgc tggtgacata tttgctgctg tttgctgcgc tgatgttgat
    26101 tatcggctgg gaaaaacgct tcttccgccg tgaggcaccg cagactgcta aggagatcgc
    26161 atgaaaaata tcgttcctga ttaccgcctt gatatggtgg gtgaaccctg cccttatccg
    26221 gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag gtgagatcct ggaagtggtg
    26281 agcgactgtc cgcagtcgat caataatatt ccactggatg cgcgtaatca cggctacacg
    26341 gtgctggata ttcagcaaga cgggccgacc attcgttatt taattcagaa gtaatccctc
    26401 attcccgctg tggcccacag cgggaacatc gttacctctg acacatagat tttacccgcc
    26461 ccctctccta ttcttttcag tatgatctca ggaggttgct atgtgtggac gctttgccca
    26521 atcccaaacg cgtgaggatt accttgcgct tctcgcggaa gatattgaac gcgatattcc
    26581 ctacgatccc gaacccattg gcagatacaa tgtcgcgccg ggaaccaaag ttcttctgct
    26641 cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg ctcccggatg
    26701 gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gcctcaacca gtcgcatgtt
    26761 taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt ttgagtggaa
    26821 aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg ctgacggaca acctgttttt
    26881 atggccgcga taggtagcat accgtttgag cgtggtgacg aagccgaagg atttttgatc
    26941 gtcactgcgg cggcagatca aggtctggta gatattcatg accgccgccc gctggttctg
    27001 tcgccagaag ctgcacggga atggatgcgg caagacattg gtggtaaaga agcctcagaa
    27061 atcgctacca gaagctgtgt gccagcaaac cagttcacct ggcaccctgt gtcgcgcacg
    27121 gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac ctgtttgttg acatcctcca
    27181 cgccctgaat gacgaggacc cctgctacgt tcaggctgtc gcctgaatca tttcggtggg
    27241 ttcctgcttc aacgagcggc ctgactgcac catctctcca caggcaagaa cggcgtgccc
    27301 cgccgctaaa atgttacgag ctccatttac atcggcgttc gctgtatatc cacatacctg
    27361 gcatctgaat ttactttgtg acaggcgatt ttctttcgct gtatgaccac agcacgcgca
    27421 acgctggctt gtgtacgctg gcggaacagc aagcacc
//
LOCUS       DAJTUP010000051        27090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTUP010000051 DAJTUP010000000
VERSION     DAJTUP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.051"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..132)
                     /locus_tag="OO468_002182"
     CDS             complement(<1..132)
                     /locus_tag="OO468_002182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6259287.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     "
     gene            complement(265..570)
                     /locus_tag="OO468_002183"
                     /pseudo
     CDS             complement(265..570)
                     /locus_tag="OO468_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            638..1253
                     /gene="tnpA"
                     /locus_tag="OO468_002184"
                     /pseudo
     CDS             638..1253
                     /gene="tnpA"
                     /locus_tag="OO468_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1581..2663
                     /locus_tag="OO468_002185"
     CDS             1581..2663
                     /locus_tag="OO468_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR6259288.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3152..3950)
                     /locus_tag="OO468_002186"
                     /pseudo
     CDS             complement(3152..3950)
                     /locus_tag="OO468_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3960..4511)
                     /gene="ybcL"
                     /locus_tag="OO468_002187"
     CDS             complement(3960..4511)
                     /gene="ybcL"
                     /locus_tag="OO468_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR6259289.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4680..5012)
                     /gene="emrE"
                     /locus_tag="OO468_002188"
     CDS             complement(4680..5012)
                     /gene="emrE"
                     /locus_tag="OO468_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR6259290.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5357..5671)
                     /gene="fliE"
                     /locus_tag="OO468_002189"
     CDS             complement(5357..5671)
                     /gene="fliE"
                     /locus_tag="OO468_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR6259291.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5885..7544
                     /gene="fliF"
                     /locus_tag="OO468_002190"
                     /pseudo
     CDS             5885..7544
                     /gene="fliF"
                     /locus_tag="OO468_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7537..8532
                     /gene="fliG"
                     /locus_tag="OO468_002191"
     CDS             7537..8532
                     /gene="fliG"
                     /locus_tag="OO468_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR6259292.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8525..9211
                     /gene="fliH"
                     /locus_tag="OO468_002192"
     CDS             8525..9211
                     /gene="fliH"
                     /locus_tag="OO468_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR6259293.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9211..10584
                     /gene="fliI"
                     /locus_tag="OO468_002193"
     CDS             9211..10584
                     /gene="fliI"
                     /locus_tag="OO468_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR6259294.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10606..10947
                     /gene="fliJ"
                     /locus_tag="OO468_002194"
                     /pseudo
     CDS             10606..10947
                     /gene="fliJ"
                     /locus_tag="OO468_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            10944..12071
                     /gene="fliK"
                     /locus_tag="OO468_002195"
     CDS             10944..12071
                     /gene="fliK"
                     /locus_tag="OO468_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR6259295.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12176..12640
                     /gene="fliL"
                     /locus_tag="OO468_002196"
     CDS             12176..12640
                     /gene="fliL"
                     /locus_tag="OO468_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR6259296.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12645..13649
                     /gene="fliM"
                     /locus_tag="OO468_002197"
     CDS             12645..13649
                     /gene="fliM"
                     /locus_tag="OO468_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR6259297.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13646..14059
                     /gene="fliN"
                     /locus_tag="OO468_002198"
     CDS             13646..14059
                     /gene="fliN"
                     /locus_tag="OO468_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR6259298.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14062..14427
                     /gene="fliO"
                     /locus_tag="OO468_002199"
     CDS             14062..14427
                     /gene="fliO"
                     /locus_tag="OO468_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR6259299.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14427..15192
                     /gene="fliP"
                     /locus_tag="OO468_002200"
                     /pseudo
     CDS             14427..15192
                     /gene="fliP"
                     /locus_tag="OO468_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            15202..15471
                     /gene="fliQ"
                     /locus_tag="OO468_002201"
     CDS             15202..15471
                     /gene="fliQ"
                     /locus_tag="OO468_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR6259300.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15480..16265
                     /gene="fliR"
                     /locus_tag="OO468_002202"
     CDS             15480..16265
                     /gene="fliR"
                     /locus_tag="OO468_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR6259301.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16550..17174
                     /gene="rcsA"
                     /locus_tag="OO468_002203"
                     /pseudo
     CDS             16550..17174
                     /gene="rcsA"
                     /locus_tag="OO468_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
     gene            complement(17218..17406)
                     /gene="dsrB"
                     /locus_tag="OO468_002204"
     CDS             complement(17218..17406)
                     /gene="dsrB"
                     /locus_tag="OO468_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR6259302.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17569..17796
                     /gene="yodD"
                     /locus_tag="OO468_002205"
     CDS             17569..17796
                     /gene="yodD"
                     /locus_tag="OO468_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR6259303.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18094..18909
                     /locus_tag="OO468_002206"
                     /pseudo
     CDS             18094..18909
                     /locus_tag="OO468_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18906..20600)
                     /gene="yedQ"
                     /locus_tag="OO468_002207"
                     /pseudo
     CDS             complement(18906..20600)
                     /gene="yedQ"
                     /locus_tag="OO468_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20771..20953)
                     /locus_tag="OO468_002208"
     CDS             complement(20771..20953)
                     /locus_tag="OO468_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR6259304.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21032..21949)
                     /locus_tag="OO468_002209"
     CDS             complement(21032..21949)
                     /locus_tag="OO468_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR6259305.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22122..23042
                     /gene="yedA"
                     /locus_tag="OO468_002210"
     CDS             22122..23042
                     /gene="yedA"
                     /locus_tag="OO468_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR6259306.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23031..23501)
                     /gene="vsr"
                     /locus_tag="OO468_002211"
     CDS             complement(23031..23501)
                     /gene="vsr"
                     /locus_tag="OO468_002211"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR6259307.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23482..24900)
                     /gene="dcm"
                     /locus_tag="OO468_002212"
     CDS             complement(23482..24900)
                     /gene="dcm"
                     /locus_tag="OO468_002212"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR6259308.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(24967..25507)
                     /locus_tag="OO468_002213"
                     /pseudo
     CDS             complement(24967..25507)
                     /locus_tag="OO468_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25547..25786)
                     /locus_tag="OO468_002214"
     CDS             complement(25547..25786)
                     /locus_tag="OO468_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259309.1"
                     /translation="MWAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKW
                     LGALVGFLFMALVGALLGGIAWYTRPRSARCRRYE"
     gene            complement(25805..26952)
                     /locus_tag="OO468_002215"
     CDS             complement(join(25805..26715,26715..26952))
                     /locus_tag="OO468_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6259310.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
BASE COUNT         6808 a         6959 c         6661 g         6662 t
ORIGIN      
        1 ggcaagttta gccacgccag catccagccc gaccattgat gctgaagggt gaaccggagt
       61 tgatacttca ctttctgcct gaatactgat gtaccactta ccgcaggact ggctgacagt
      121 gacatttttc acaacacccg tgacctgacg gctgttccgg tagcgcatcc agccaagttt
      181 tggcagaaaa atacggctgt tttcctgagc gagcttaacg ccctgcggat agcggaatgc
      241 atcattctgt caccgctttt tgaatcaggg gaacgccgag cgcttctgga aaaagttttt
      301 gcaggcctgc tcagggtctt tcagtgactg ttgcaatggc tgtgacggag catctttaat
      361 ccattgcgtt tcagtggcat ttttccactc aaccaatcag gaagccattt tcgcgtaagg
      421 gatgtctttg ttaccggcct tatgattctc attctgacgt gccagagtac gactgaaaac
      481 gaaacgacat gcgcctgcga agcgcctcat ttcacgcgcc tgttgaccac cgggtcttaa
      541 ccggaatttg aatacctgta gtcgtttcac agggcaaatt atactttggt ctgtgaaaaa
      601 aactgatatc cgacgcggaa ggcactgtgt tttcctgatg catgctcacc gggtctttgt
      661 taccaagtac cgacgccaga tttttgacca tgacgcgaca gaaaaactac gcacttactt
      721 ttcaaatgta tgtgcttatt ttgaagctga actggttgaa atggatggcg aaccagatca
      781 cgtccatctg ttaattaact atccccccaa actggcgata tccagtctgg taaacaaaca
      841 gcctcaaagg cgtatcgggt aggttactgc gacgggatcg accagatatt gctgccaggt
      901 attaccgcaa agatgttttg tggcgtcccg gctattttgc cagtagctgc ggaggtgcgc
      961 caatatccgt catccgccaa tacattgaac aacagcaaac acctggtcag atgaaaaaac
     1021 gagccttata tccccgccct gaaggacggg cttttacggt gcactggata agcctggaga
     1081 ttatctacac ctgcatggac atcattgtac tatgccccgt acatgctcca tcacatcctc
     1141 aacatcaaag tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt
     1201 acgcaaaaaa aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc
     1261 tcaattacat taataaatct aaataaatat catgcaaata atttccatgg ttatttttag
     1321 gctatttaaa ttaaaaaaat aaattatgag tcaatcataa ataacaacct ttaatataat
     1381 ttgactacca aatttatatt cgttcacaaa atagtcatgc attgtgtgca atagaaacag
     1441 ttcagataaa aatagggatc tacttcacaa tttaaatgag aaactaaaac ttacatcttg
     1501 aaataatctc attgattgga tgaatatttg tagtgcagtg acatcatttt taaataatag
     1561 ttcaaataaa agggctcacg atgaaaaaat taacagtggc aatttctgct gtagctgcat
     1621 cagtactgat ggcgatgtct gctcaggcag cagagattta caataaagac agtaacaagc
     1681 tggatctgta cggaaaagtt aatgctaagc actacttctc ttctaacgat gcagatgatg
     1741 gcgacacaac ttatgttcgt ctgggcttca aaggcgaaac tcaaatcaac gatcaactga
     1801 ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg tgctgaatct caaggttctt
     1861 ccaaagataa aacccgtctt gccttcgctg gcctgaaatt cggtgactac ggctccatcg
     1921 attatggccg taactacggt gtagcatacg atatcggtgc atggaccgac gttctgccag
     1981 aattcggtgg cgatacctgg acccaaacag atgtgttcat gactggtcgc accacaggtg
     2041 ttgcaaccta tcgtaacaac gacttctttg gtctggttga tggtctgaac tttgctgctc
     2101 agtatcaggg taaaaatgac cgcactgacg taactgaagc taacggtgat ggtttcggtt
     2161 tctccactac ttatgagtat gaaggattcg gtgtaggtgc aacctatgct aaatctgacc
     2221 gcactaatga tcaggttatc tacggtaaca acagcctgaa tgcatctggt caaaatgctg
     2281 aagtatgggc agctggtctg aaatatgatg cgaacaacat ctatctggct accacctatt
     2341 ctgaaaccca gaacatgact gtttttggta ataaccatat tgccaacaaa gcacaaaact
     2401 tcgaagtagt tgcacaatat cagttcgact tcggtctgcg tccgtccgtt gcttacctgc
     2461 aatctaaagg aaaagacttg ggtgcgtggg gtgatcagga cctgattgaa tatattgatg
     2521 taggtgcaac ctattacttc aacaaaaata tgtccacttt tgttgattac aaaatcaacc
     2581 tgattgataa gagcgatttc acgaaagcat ctggcgttgc taccgatgat atcgttgctg
     2641 taggattggt ttaccagttc taatctgatt actcaaagat atgttgtggg aggctttgcc
     2701 tccccaacat ataagtggcg ctctcaagcc actttcctta gaagcactat cttgcttctg
     2761 actatataaa ttacccttta ttttctggga cgtttcaacg ccccattttt aataatactc
     2821 agtgaacaat taacgtatca attagattta ataacaacga tatccgtacc taacctgata
     2881 tctaatgtca taagcatccc tttaattatc ccctcaacct cttgtaaatt tcccactctt
     2941 gagctccaca tagcccaaac gccggatgca aaaaaaatca tacgtgcatc tctgaaattg
     3001 tgatggagga agtgagtatc aattatatgt gcttataaac gttcccgatt ggcacagaag
     3061 tcaccggatt gttcaggctc caataggttg agtatgtcgt atttattttc caaaataaaa
     3121 atcgatgctg ttgctcctta ataaaatact tttactctct ggacgctttt cttagctctc
     3181 ttggtgttat attaaaacga ttatggaacc tttcagtaaa acgagaagga cacttgtaac
     3241 cattttccct ggcaatctcg ctgatgggtt ttactgtcgt ttgtatagca ggacaacgca
     3301 ttatttaacc tcacgtcgtc caatatactt tgaaaactta ttccctcgct tgccagacgg
     3361 cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc tgtccatttt
     3421 gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg tttactatct
     3481 gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc cattgctaag
     3541 tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg ctcccaacaa
     3601 tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat ttgatcagtt
     3661 atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag catttctgca
     3721 agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca gggaaataat
     3781 attatctgat tattatcaac agttagacta tgcgtctccc aattgataac tttttttccc
     3841 tgacggatac gacataaagc tggcataaga ggcttaaccc tgtgaatctc atgatgttta
     3901 tgcatccgta tttcttcaat ctttaggtta gacttatctc ttgccagcat actcttaccc
     3961 tattttatct cataaactgg tgttatctca gcggttgcaa ttttattggc attaagcata
     4021 taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt ttttagagcc
     4081 catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc tccaccaaat
     4141 cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt tgtaccatca
     4201 cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc aacagtccag
     4261 tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc aaagcttttg
     4321 gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc ttcacaccca
     4381 aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc attactagtg
     4441 acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact tgaaacgata
     4501 agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga tgtgccttga
     4561 tctcattatg cataaaggcg caatgatcgc gcaaatgcaa tcataattat aaaggtgccc
     4621 cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag aattattttt
     4681 taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca aatcaacatc
     4741 atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga aagcaaacta
     4801 atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg tatataagcc
     4861 agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc aacagagggc
     4921 cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac ctctgcaagt
     4981 attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt catactggag
     5041 agaattgtac tacagtttga acacaactca ccgttttcat cctggtgtat gtgtatccaa
     5101 ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag gaaccccagg
     5161 ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt tatcgcgttt
     5221 acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac cagaaattga
     5281 gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa caagcatagt
     5341 caacaggtta cacaacctac acctgcatgg acatcacttc ctgatacgcc gccaccagct
     5401 tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata tcggtcatca
     5461 catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc gtgcgggcga
     5521 ctgtttgtgt atcgcttatg cgatcgagcg cggcgtgcag ctgcccggca aaactaatgg
     5581 tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt aactggctga
     5641 taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt tctggtaaca
     5701 aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa actggtttat
     5761 tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg agtgtgctga
     5821 cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag ccacgaagtg
     5881 cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc gcctgcgtgc
     5941 gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca tggtcgcact
     6001 gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt ccgatcagga
     6061 tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct tcagcgaagc
     6121 cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgg cgcaacaagg
     6181 tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat caggaaaagt ttggtatcag
     6241 ccagttcagc gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat
     6301 cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt
     6361 attcgtccgt gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg
     6421 cgcactcgat gaagggcaaa ttagcgctat tgtacatctg gtttccagcg cggttgctgg
     6481 tctgccgcct ggaaacgtca cgctggtgga tcagggcggg catctgttaa cccagtccaa
     6541 taccagcggg cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg
     6601 tattcagcag cgtattgaag cgatcctgtc acctattgtt ggtaacggta atattcacgc
     6661 ctaggtcacg gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc
     6721 taacggtgat gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag
     6781 cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa
     6841 taacgcgcca atcagcacgc ctccgacaaa tcaaaataac cgccagcagc aggcgagcac
     6901 caccagcaat agtgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga
     6961 tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt
     7021 agtggtgaat tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat
     7081 gaagcaaatt gaagctctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc
     7141 gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa agcggcggag cactgccgtt
     7201 ttggcaacag caagtgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc
     7261 tggtactgct ggtggcgtgg ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc
     7321 gcgccgaggc ggtgaagact gtgcagcaac aggcgcaggc ccgcgaggaa gtggaagatg
     7381 cggtggaagt ccgtctgagc aaagacgaac aactccaaca acggcgcgct aaccaacgac
     7441 tgggggcaga agtcatgagc cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg
     7501 tggcgctggt cattcgccag tggataaata acgatcatga gtaacctgac aggcaccgat
     7561 aaaagcgtca tcctgctgat gaccattggc gaagaccggg cggcagaggt gttcaagcac
     7621 ctctcccagc gcgaagtgca aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc
     7681 aacaagcagc taaccgatgt gctggcggag tttgagcaag aagctgaaca gtttgctgca
     7741 ctgaatatca acgccaacga ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa
     7801 cgtgccgcca gcctgctgga agatattctc gaaacccgcg ataccgccag cggtattgaa
     7861 acgctcaact ttatggagcc gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa
     7921 attatcgcca ccattctggt gcatctgagg cgcgcccagg ccgccgatat tctggcgttg
     7981 ttcgatgaac gtctgcgcca cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag
     8041 ccagccgcgc tggccgaact gaccgaagta ctgaatggct tgctcgacgg tcagaatctc
     8101 aagcgcagca aaatgggcgg cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg
     8161 cagcaggaag aagccgttat taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa
     8221 atcatcgacg agatgttcct gttcgagaat ctggtggatg tcgatgatcg cagcattcag
     8281 cgtctgttgc aggaagtgga ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag
     8341 ccactgcgcg agaaattcct gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac
     8401 gatctcgcca agcgtggtcc ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt
     8461 ctgctgattg tgcgccgtct tgccgaaact ggcgagatgg tgattggcag cggcgaggat
     8521 acctatgtct gataatctgc cgtggaaaac ctggacgccg gacgatctcg cgccaccacc
     8581 ggcagagttt gtgccaatgg tcgagtcgga agaaaccatt attgaagagg ccgaacccag
     8641 ccttgagcag caactggcgc aactgcaaat gcaggcccac gagcagggtt atcaggcggg
     8701 gattgccgaa ggtcgccagc aaggtcacga acagggctat caggaaggac tggcccaggg
     8761 gctggaacaa ggtctggcag aggcgaaggc tcaacaagcg ccaattcatg cccggatgca
     8821 gcaactggtc agcgaatttc aaactaccct tgatgcgctt gatagtgtga tagcttcgcg
     8881 cctgatgcag atggcgctgg aggcagcacg tcaggtgatc ggtcagacac caacggtgga
     8941 taactcggca ctgatcaaac agatccaaca gttgttgcag caagaaccgt tattcagcgg
     9001 gaaaccacag ctgcgcgtac acccggatga tctgcaacgt gtggatgata tgctcggcgc
     9061 aactttaagt ttgcatggct ggcgcttgcg gggcgatccc accctccacc ctggcggatg
     9121 taaagtctcc gccgatgaag gcgatctcga cgccagtgtc gccactcgct ggcaagaact
     9181 ctgccgtctg gcagcaccag gagtggtgta atgaccacgc gcctgactcg ctggctaacc
     9241 acgctggata actttgaagc caaaatggcg cagttgcctg cggtacgtcg ctacgggcga
     9301 ttaacccgcg ctaccgggct ggtgctggaa gccaccggat tacaattgcc gctcggcgca
     9361 acctgtgtca ttgagcgcca gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt
     9421 ggctttaacg gtcaacggct gtttttaatg ccgctggagg aagtggaagg tgtcctgccc
     9481 ggcgcgcgtg tatatgccaa aaacatttcg gcagaagggc tgcaaagcgg caagcagttg
     9541 ccgctcggtc cggcgttatt aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc
     9601 ctgcccttcc ccgatacgac ggaaaccggt gcgctgatta ccccgccatt taacccgttg
     9661 caacgtacac cgattgaaca tgtgctggac accggcgtgc gcccaatcaa tgccctgctt
     9721 accgtcggac gcgggcagcg tatggggctg tttgccgggt cgggcgttgg taaaagtgtg
     9781 ctgctgggga tgatggcacg ctacacccgc gccgatgtca ttgtcgtggg tttgattggt
     9841 gaacgtgggc gcgaagtaaa agattttatt gagaatatcc tcggtgccga aggacgtgca
     9901 cgctcagtgg tgattgccgc tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc
     9961 gcctatgcca cgcgcattgc cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt
    10021 atggactccc tcacccgcta cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa
    10081 ccacctgcca ctaaaggtta tccaccgtcg gtgtttgcca aattaccggc actggtcgag
    10141 cgtgccggta atggcattag cggcggcggc tcgattaccg cgttttatac cgtgctcacc
    10201 gaaggcgatg accagcagga ccccattgct gactccgcgc gggcaatcct cgacggtcac
    10261 attgtgctgt ctcgccgact ggcggaagcc gggcactatc cggctatcga tattgaagcg
    10321 tcgatcagtc gcgcaatgac ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc
    10381 ttcaaacagc tgttgtcgag ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat
    10441 gccaaaggca gcgatccgat gctcgataaa gccatcgccc tgtggccgca gctggagggc
    10501 tatttgcaac aaggcatttt tgaacgcgcg gactgggaag cgtctctcca ggggctggag
    10561 cgtattttcc cgacagtgtc ataacccagg agataacggc agatggcgga agaacagctc
    10621 aaaatgttga tcgattatca gaatgaatat cgcaataacc tcaatagcga tatgagtgcc
    10681 gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc
    10741 attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac
    10801 agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc
    10861 acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag
    10921 cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg
    10981 acaccaccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt
    11041 tgagcgaagc attagcaggc gagacaacaa ctgacaaagc ggccccccag ttgctggtag
    11101 caacagataa gcccacgaca aaaggcgagc cgctggtcag cgatattctt gccgatgcgc
    11161 aacaagcgga tttactgatc cctgtggatg aaacacttcc tgtcatcaac gacgaacaat
    11221 ccacatcaac accgttaacc accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa
    11281 atacgacaaa agacgaaaaa gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg
    11341 ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat gcaccgtcaa
    11401 ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacta
    11461 cagcacagcc tgatgacgcc cccggcacac cagctcagcc attaacaccg ctggtagcag
    11521 aagcccagag taaagcggaa gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc
    11581 cgctaatcac tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg
    11641 caccgctggg ttctcacgaa tggcaacaat cattgagcca gcatatttcg ctgttcaccc
    11701 gccaggggca acaaagtgca gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa
    11761 tttccctcaa agtggatgat aaccaggcgc agatccagat gatttcaccg catcagcacg
    11821 tacgcgccgc cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca
    11881 ttcagttagg gcaaagcaac attagtggcg aaagctttag tggtcagcag caggccgctt
    11941 cccagcaaca gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagacgacg
    12001 atacgcttcc ggttcccgtc tctttacaag ggcgtgtaac aggtaacagc ggcgttgata
    12061 ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct
    12121 caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac
    12181 tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat
    12241 taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc
    12301 tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt
    12361 tacggtcaat ttgggcgatg cggatcgcgt gctttatatc ggcataaccc tgcgactgaa
    12421 agatgaagca acccgctcgc ggctgagtga gtatttgccg gaagtccgta gtcgcttgct
    12481 gttactgttt tcacgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct
    12541 gattgccgag attaaaacca cactttccac cccgcttgtt gccgggcaac cgaaacagga
    12601 tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc
    12661 tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga
    12721 cagccagtat tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg
    12781 ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc
    12841 gtatggggct gttcaacctg ctgcgccgta gcccggacat taccgtcggg gccatccgca
    12901 ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc
    12961 acctgaaacc gctacgcggc acagggctgg tagtgttctc gccgagtctg gtgtttatcg
    13021 ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt
    13081 ttacccatac tgaacagcgc gtcatcaacc gtatgttgaa gctggcgctg gaaggctata
    13141 gcgacgcctg gaaagcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg
    13201 tggaatttac caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg
    13261 tggagattgg caacctgacc ggcgaattta atatctgcct gccattcagc atgatcgagc
    13321 cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact
    13381 ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggaactggtg gccaactttg
    13441 ccgatatctc gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga
    13501 tagaaaaacc cgatcgcatc atcgcccatg ttgacggcgt cccggttctg accagtcagt
    13561 acggtactct caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa
    13621 attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa
    13681 cggcgcaatg gacgatctgt gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa
    13741 aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca
    13801 ggatatcgac ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg
    13861 gatgaccatc aaagagctgt tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct
    13921 ggcgggcgaa ccgctggata ttctgatcaa cggttattta atcgcccagg gcgaagtggt
    13981 ggttgttgcc gataaatatg gcgtgcggat caccgatatc attaccccgt ccgagcggat
    14041 gcgccgcctg agccgttagt gatgaataac catgctactg tgcaatcttc cgcgccggtt
    14101 tctgctgagc cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc
    14161 gctgctgcct ggctggtaaa acggctggga tttgccccta aacgcactgg cgttaacggt
    14221 ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg
    14281 gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt
    14341 cccccttcag caccaacgga agagataccg cagaccgatt ttcagtcggt catgaaaaat
    14401 ttgcttaagc gtggcgggag atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc
    14461 tctggctgat tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctac
    14521 ctggcgggta tcaccagcca gccgctacct ggcggtggac aaagctggtc gctcccggtg
    14581 cagacgctgg tgttcatcac ctcgttgacg ttcattccag cgattttact gatgatgacc
    14641 agtttcaccc gcatcattat tgtttttggt ttattgcgta acgcgctggg aacgccctcc
    14701 gcgccaccta accaggtatt gctggggctg gcactgtttt tgaccttttt tattatgtcg
    14761 ccagtgatcg acaaaattta tgtcgatgcg taccagccat tcagcgaaga gaaaatctca
    14821 atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg agtttatgct gcgtcagacc
    14881 cgcgaggcag atttagggtt gtttgccaga ctggcgaata ccggcccgtt gcaggggcct
    14941 gaagctgtgc cgatgcgcat tttgctcccg gcctacgtga ccagcgagtt gaaaaccgca
    15001 tttcagatag gcttcacgat tttcatccct tttttgatta tcgacctggt gattgccagc
    15061 gtgttgatgg cattggggat gatgatggtt ccccccgcca ccattgctct gccctttaaa
    15121 ctgatgctgt ttgtactggt ggatggctgg caattgctgg tcggttcgct ggcgcagagc
    15181 ttttacagct agagaggcaa aatgacacct gaatcggtca tgatgatggg aactgaagcg
    15241 atgaaagtcg cgctggcact ggcagccccg ctgttgctgg tagcgctggt cacgggcctt
    15301 atcatcagta ttttgcaggc agccacgcag attaacgaaa tgacgctgtc gtttattccg
    15361 aaaatcatcg ccgtatttat cgccatcatt attgccggac cgtggatgct caatctgttg
    15421 ctggattacg tccgcacctt gttcactaac ctgccgtata tcatcgggta gcccgtacaa
    15481 tgatgcagga gacaagcgat caatggcttt cctggttaag cctgtacttc tggccgttac
    15541 tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag cgaacgcagc gtgccgaaac
    15601 gggtaaaact gggtctggca atgatgatca cgttcgccat tgccccatca ttacctgcca
    15661 acgatgttcc tgttttttcg ttctttgctc tgtggctggc cgtacagcag atcctgatcg
    15721 gcattgcgct tggttttacc atgcaatttg cctttgccgc tgtgcgaacc gctggcgaaa
    15781 ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt cgatcctggc agccatctca
    15841 atatgcccgt tttagcgcgc atcatggata tgctggcatt actgctgttc ctgacattta
    15901 acggtcattt atggttgatt tcactgctgg tcgatacctt tcacaccctg ccgattggta
    15961 gcgaaccgtt gaacagcaat gcgtttctgg cacccaccaa agcagggagt ttgattttcc
    16021 ttaacgggct gatgctggcg ttaccgctca ttactctgtt gctgaccctg aatctggcat
    16081 taggtttact gaatcgtatg gccccgcaat tatccatttt tgttattgga tttccattaa
    16141 ctctgactgt cggcatctct ttaatggcgg cattaatgcc gttaattgcg cctttttgcg
    16201 aacatttatt cagtgaaatt tttaatttgc tggctgatat tattagtgaa ttaccattaa
    16261 tataattccg taacgtttat catgctaccc taaggattat ccgaaaaata atatctgcga
    16321 acatcttcca ggatactcct gtagcgaaat atttgtttta agctcactca catatcgcaa
    16381 catttacttt aagacaattc caggcaaatt atacaacact ttacgggata gtaagtccgc
    16441 ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt gttccgttta gtcatgatga
    16501 aatattcagg taaggggaat tatcgttacg cattgagtga gggtatgcca tgtcaacgat
    16561 tattatggat ttatgtagtt acacccgact aggtttaacc gggtatctgt tgagtagagg
    16621 ggttaaaaaa agagaaatca acgacattga aaccgttgat gaccttgcca tagcttgtga
    16681 ttcacagcgt ccttcagtgg tgtttattaa tgaggactgt ttcatccacg atgcttctaa
    16741 cagtcagcgc atcaagcaca tcattaatca acatcccaat acgttattta tcgtttttat
    16801 ggcaattgcc aatgttcatt ttgatgaata tctattggtc agaaaaaaat ttattgatca
    16861 gttctaaatc gattaaaccg gaatctctcg acgatatcct tggcgatatt ctgaaaaaag
    16921 agacaacgat aacctcgttt ttaaatatgc cgacgttatc attgagccga accgaatcga
    16981 gtatgttgcg aatgtggatg gcaggtcagg gaaccattca aatctccgac caaatgaata
    17041 tcaaagccaa gaccgtttca tcgcataaag gtaatattaa acgtaagatc aaaacgcata
    17101 ataaacaggt tatctaccat gtcgtccgac tgacggataa tgtgactaat ggtatttttg
    17161 tcaacatgcg ctaacacatt ctgactggtg gtttcccacc agtcaggctg aataagatta
    17221 ctctgctttc tcaacaaaga taccgtcctg atgccctgct tcattaaaga accagatgcc
    17281 gagcgggtag tcttccagcg caaccaggta cattgtgcct tcactaaact cctcaactgc
    17341 cagtaccact ccaggacgac gcggaccgcc atccgttttg actgttaccc gatcattcac
    17401 cttcatcgtt ttcctcctgt ggctttgtgc cagtgtagaa caatttcgtt tttctggcag
    17461 cgccaagcgc gcgcgagtgc tgattttctc gacggtctat acttaagaga tgccaggcgg
    17521 acttaacgac tggcggcaac aacagagtaa cggttgcgag gaaagatgat gaaaaccgca
    17581 aaagtgtaca gcgataccgc aaaacgtgag gtcagcgtcg atgtcgatgc cctgctggcg
    17641 gcgatcaatg aaattagcga aagcgaagtt catcgcagcc agaacgattc tgaacacgtt
    17701 agcgtcgatg gacgtgaata tcatacatgg cgtgaattgg cggatgcctt cgaactggat
    17761 attcatgact tcagcgtctc tgaagtgaat cgttgaatgc acaataaaaa aatcccgacc
    17821 ctgagggtgt cgggatgaaa cttgcttaag caagaagcac ttaaaaaatt cgttacacca
    17881 ggaaatctga tgtgttcatc accttatctg caattttttt cgctgacaag aaaatattcg
    17941 cgctatgaat gattaacgct acttatgaat agaaatgtga cgcgcgtcac cccttggtgg
    18001 ctctggtgtc aacgggatcg ctcccgaaaa aatcatcttt gattatttgt gctgttttga
    18061 gaatcatcct ggtaagggga gtactgcgcg accatgtttt caattcaaca accactactg
    18121 gtttttagcg atcttgatgg caccctgctg gacagtcata gttatgactg gcaaccggca
    18181 gccccctggc tcagccgttt acacgaagcc aatattcccg tcattctctg tagcagtaaa
    18241 actttagcgg aaatgctgta cttgcaaaaa atgttggggc tacaaggctt accgctgatt
    18301 gcagagaatg gcgcagtgat ccagcttgct gaacaatggc aggatataga cggttttcca
    18361 cgcatcatct caggtattag ccatggcgaa atctgccagg ttttaaatac gctacgcgag
    18421 aaagaacatt ttaaattcac gacttttgat gatgtcgacg atgcaaccat tgccgaatgg
    18481 acgggattaa gccgtagcta ggcggcgctg acgcagctgc atgaggcgtc ggtaacgcta
    18541 atctggcgcg acagtgacga gcgtatggca caatttatcg ctcgtctgaa cgaactgggc
    18601 ttgcagttta tgcagggtgc gcgcttctgg cacgtactgg atgcctctgc cggaaaagat
    18661 caggctgcca actggattat cgcgacctat caacaattgt caggcagacg cccaaccaca
    18721 cttggcctgg gcgatgggcc aaacgatgcg cctttactgg aggtaatgga ttacgcggtg
    18781 attgtgaaag ggctaaaccg tgaaggggta catctgcatg atgaggatcc ggcccgcgtc
    18841 tggcgaacgc agcgtgaagg accggaaggc tggcgtgaag ggctggacca ttttttctcc
    18901 gcccgttaag cgttatcgct cgcgaatacc cgattgcgcc cggcctgttt tgcgaggtaa
    18961 agccgacggt cggccaatga ctggagttgt tcaaaatcat aatcaccggt ttcctcgctg
    19021 ctacttaccc ccagcgaggc actgatgcgt atcgttgtac tcttggcgat cagcatctct
    19081 ttttcattta acttcaggcg aatacgttct gcgacctccg cagcctgcgt cagattcgcg
    19141 cctggcagaa tcacacaaaa ctcctcacca ccgacccgcc cggcaacgtc ctgcgcacgc
    19201 aaggaactgc taattaatcc ggcagcatga gaaagaacac ggtcgcccgc ctgatgacca
    19261 aagcggtcat taatcgcttt aaaatggtca agatcgacct gaatgacaga aaaaggatgt
    19321 tggtgcgtct gacacaattt agcgagcgga cgggcttttt cgaacagtgc gccacggtta
    19381 tataaacgcg ttaaggtgtc gtgccacgcc tgccactgca acgagctttg cagaacatac
    19441 atgttgctga ccatccggcg aatcacatac caggagagga gtaacatggt ggtaaagagc
    19501 gcccacagca gggttaatgc aatgctgata ctgccgaaat cgccgcgcac gccttcgctt
    19561 agcgtatgga cgcgcgccag cacaccgtcg aaatggtcca gacgttccca gctaacatag
    19621 cgactgtcca tacgaatgcc gccccgcgtg tcatgttcca ttgcctgcgc cagcaaggcc
    19681 agttcacgag gatcaaaaat attccctgtt ggatggtcag gattggaaga ggtcaaaaat
    19741 ctcagcttac tgtcatagag ctgatactca ccatcgaggt ttttatcgat ggcgtttctt
    19801 aaaaattgct gcatagtacg cacgggaata ctcatcccca gcacgccata ccagtagtta
    19861 ttactgtcta ccggaacact gacggtaacc tgcggttcag tattgctggc gtgttccggt
    19921 tgcgaagtga accagcgtac cgcgcggtga cgattttctc gttgcgaatg gccgataaac
    19981 cagggttggg tgacatagcc gtaataacgc gttggtacat tgcgcgtaaa caaggtcggc
    20041 tgcgtcgaaa cgtaaaatcc agcacgcgag acatacatcg cctgttcaac catcgacgag
    20101 gagttgtgcg ccagtcgcag caagtaacca acttccagtg cagcggtaat ttcattgtcg
    20161 aggctttcat tttcgcgaga caggaaattc ccctcgctga ctaatgcatc cgatacgcca
    20221 ttgactggca gggtgcgccg tcggttgagt tcgatttgcc aggcgtgctc gtcgcgatgc
    20281 tgttcgaact cggttacggc attacgcaga gaggtgaaat ccagtggcgc cacgagagct
    20341 tcgcgcatgc cattacgcaa aaagatcagt ttgtcgacgt tatactgcaa atgcttatcg
    20401 agcgcgttgg ctacgttttc cagatgatta cgctagctgg agatataggc atcttccagc
    20461 accaccactt cacgccaggt gagcaaggtg gaaaaaagca ataccacgat aaagcagaga
    20521 ttaacgacat gaccgggccc caggcggcgt gcgagttttt tcaaccagct ctggttttcc
    20581 atttttgtct cgtgctgcac cctgacgact cccaacctgc ttttttatga ttctggcata
    20641 acccggcaat agcgtaccgc aaaacagcga taattttata atggtggcat gataagacgc
    20701 gactatcatt cattgcaggc attgggacac caggccggat gtgatccggc ctggtgttaa
    20761 tgataccctt tcaaacttct tccgcagaac gactcccctc ccccggaaca agatcggttt
    20821 catgaaaagc ttcccgcttg accccgtaac cgtcatacca acgacactca accataccgc
    20881 tggagtatcc agtgacaatc atccgcgggc cgccctcttt aaccgtaact tcctcactaa
    20941 ccataaagct catactcgcc tccttttttc gagtgaaact tgttcacctt agttgaagat
    21001 ggcgaatttt gcataatagc cagtgcgagt attaatgtgc ctgaccgcgc attttcgcta
    21061 ctgctttcac tcccagcacc acgatgccgc cgatgataaa tccaagaatc agatttaaaa
    21121 cagtcggtaa tatcatcgcc accactgcac tttgctgccc ggcgatatgt tcaatagcgt
    21181 gatgcagcgg cgcaatacca tgtaccacaa tcccgccgcc gacgaggaac atcgccagcg
    21241 tgccgacaat cgataatgct ttcatcagcc aaggcgcgat aatcaataac cctttaccta
    21301 atgcctgcat cagcgcgcta gatttttccg ccagccaata ccctaggtca tcaatcttaa
    21361 cgataacccc taccagaccg tagacgccca cggtcactac cagcgcgatg cctgaaagca
    21421 ccagcacctg attaagcaac ggcgcctctg ccacaatccc cagcgtgatg gcgacgattt
    21481 ccgcagacaa aataaaatcg gtacgaatcg cccctttaat tttgtccttt tcaaacttca
    21541 gcggatcctg cgccgccagc ttctccagac gctgctggct ctgcgctgga tcttctttat
    21601 gtttacgcgc ctccagcata tgcagcactt tctctacccc ttcaaagcag agaaacgcgc
    21661 caccaatcat caacagtggc gtaatcgccc acgggataaa cgcactgatg atcagcgcca
    21721 gcggcaccag aatcacttta ttgatcagcg atcctttcgc cacgccccag accacgggaa
    21781 gttcccggtt ggcccgcacg cctgaaactt gttgcgcatt gagcgataaa tcatccccta
    21841 atacaccggc ggttttcttc gccgccagtt tgcccatcac agagatatcg tccagcagtg
    21901 tggcgatatc atcgagcaac gtcagtaaac tactcccggc caaaagcatt ccctctcatt
    21961 tttattgttg aagggtgtaa gtatgaagca aaaacggcag ctctgatact tgcttcacag
    22021 gtcaacattt atttaacaat caagtgcaat tatatctctc gccagcagaa tgattttcac
    22081 gtttactatg aaccaccttt ttatttccgc cgtgagggat tatgcgtttc cgccagttgt
    22141 taccgctttt tggcgcgctg tttgcgttgt atatcatttg gggctcaacc tattttgtca
    22201 ttcggattgg cgtgggaagc tggcctccgt taatgatggc gggcgttcga ttccaggcag
    22261 ccggtatttt attgctggca tttttgctac tgcgcggaca caaacttccc ccgctacgtc
    22321 cgctgctcaa tgccgcgctg attggcctgt tattgctggc tgtcggtaat ggcatggtga
    22381 cggttgccga acatcaaaat gttccttccg gcatcgccgc cgtagtgatt gcaaccgtgc
    22441 ccctctttac cctgtgcttc agtcgcctgt ttggcattaa aacgcgcaaa ctggaatggg
    22501 tgggtattgc cattgggctt gccggaatca tcatgctcaa tagcggtgga aatttaagcg
    22561 gcaatccgtg gggcgcgatt ctgattttaa tcggctcgat tagctgggcg tttggctcag
    22621 tttatggctc gcgcattacc ttacctgtag ggatgatggc gggtgcgatt gagatgctgg
    22681 cggcaggcgt ggtgttaatg atcgcatcga tgattgcggg tgaaaaactg acggcgctcc
    22741 cttccctgtc aggcttcctt gcggtcggct atctggcgct gtttggttcg attatcgcca
    22801 ttaacgctta tatgtattta atccgtaatg tcagtccggc tctcgccacc agctacgcct
    22861 acgttaaccc ggtggtcgcg gtcttgctgg gtacgggact gggtggcgaa acgctgtcga
    22921 agattgaatg gctggcgctc ggcgtaattg ttttcgcggt ggtactggtc acgttgggaa
    22981 aatatctctt cccggccaaa cccgttgtcg cgccagttat tcaggatgca tcaagcgagt
    23041 aaatgaatcc cctgcgtgtc gatctgcgcg ctggcacctt cgccgcagat ccactcttcc
    23101 agacgctcgg taagcgccgc atccgtcagc ttctcgcgcc tacgtaacgc acactcccag
    23161 acaatcagta cgcgccagcc gagctcctgc aagcgactga tatcgcggcg atcgcgttca
    23221 acatttttac ctatcttctc cagccagaac tcagttcgag tcgcaggcac tttaaacaga
    23281 tagcagtgat gatgatgcca gaagcagccg tgggtaaata tcacgcagcg atattcatca
    23341 acgacaaaat ccgggcgtcc gggcagactg gcgtcctgaa cgcgaaacgc aaggccctgc
    23401 ccggttaaca aactggcgag gcgcttctca atcgccgtat cacgcgtggc aatcgcgcgc
    23461 atatttttgc tgcgggtggc cttatcgtga acgtcgacca tgttgtgcct cttgctgacg
    23521 caacgccacc gcctgtttga tttttggctc aagcagtttt gccaccgcgg caaagaccgg
    23581 cacgaccacc gagttgccga actggcgata ggcctgagtg tccgaaaccg gaatgcggaa
    23641 tttcgcttct cccggcgctt caaaacccat taagcgcgcg cattcacgag gcgttaaccg
    23701 acgtggacga tgttgctgat tcagcggatc gtcaaagtct ttctcacccg tggccatatc
    23761 ccaaccgcga tcgattaaaa tttccgcgcc atctttgtaa taacgagcag acagcgtacg
    23821 ggtgacgctt tgcggattgt tcggataaac cattccataa ccgaagccgt taccgcgcgc
    23881 ctgatgtttt ttcgcatatc gatagaggta cttccacagc accggcgtca ggatatattt
    23941 cgcctcaacc atcgggtcca gcagctgcgc cagcgtcact cgctgtgcag ggaaacattc
    24001 gctgatatca cgcagagtaa aatcggcttt cagattcaga tcgcgacgaa aacccaccag
    24061 cacgatgcgt tcacggtgct gcggcaggaa atgtttacca tcgatgattt tcggatcgtc
    24121 cggcccgtta tcttctgcat cggcaacgtc atagcccagt tcgtccagcg tctgcatgat
    24181 gatgcggaac gtttttccct tatcgtgact cttcaggttt ttgacgtttt cgagcacaaa
    24241 catcgccgga cgacgcgcgt cgataatgcg caccacatca aagaacagcg tgccctgggt
    24301 atcgcaggca aaaccgtgcg cccgcccgag cgagttcttt ttcgatacgc cagccagcga
    24361 aaatggctga caagggaaac cggccagtaa aacatcgtgt tcaggaatgt gttgacgaat
    24421 atgttccgcc gccgcctcat cactcacgcc ttcttgatga ctgagggtga tgtcgcggat
    24481 atcttcatta aaatgatgcg tcgccgggtc gcaataatgg ttggctttat aagtgcgtac
    24541 cgcatgtttg ttccattcgc tggtaaacac gcactgtcca ccaatcgatt caaaaccacg
    24601 acggatgccg ccaattccgg caaatagatc gataaagcga aacgcataat gcggatgatg
    24661 ttccggtggt ttgggtaaca acgtttgcag atgggcaaac tctttctcac ttaaacggtg
    24721 ccatgccgag tcattcgcca gcgcacgttt taaaattgcc gcgctccagt gattctcacc
    24781 cacaccatta agctgcgcca ccagcatttt aacatcataa atttgcagca gtttctccag
    24841 cattgcctgt gcggcattcc cggtgctgta tgaatcggtt actgatatat tttcctgcat
    24901 agatttaacc ggccatctga gatggccgac aggttaacat aattactgcc gtttagggcc
    24961 acgcgctcag ccagcgggtg aaaacgcatc tatcaccttg tgatcgatac cttcattctc
    25021 ccccgccagt tcggcaccga gcttcgccat caaacagcgc caagctcaat gcccctgaaa
    25081 cggcaaatac gcgcgccagg ccaatcgccc ccagggcttc cagccgatcc gcatcctgga
    25141 cgattttagc ttccgttgtt aagggggcga tttgcgcgct gaaactgtga gcggcaatgg
    25201 catgacaaac ggcctcaatt ttctccgccg gaaattgcac gaactcttca cggagcaggc
    25261 gacgcgtctc ttctgccgcc aggattgaag aacgctgccg ttgcggatga tttttcgcca
    25321 ggctcacgat atcgtgaaaa taacaggcgg ttaaaatcac cagcatatcg acatcgtcat
    25381 ctgccgccag tttctgtgcc gtagcccaga cacggcgaaa atgacacagg tcatgcgccg
    25441 cgtcctgatg ttgatgatgg tttttcaacc agttctcgaa ctatgcctgc cagtgttgta
    25501 actccatacg cgctccgttg ccaaagagtc tcaaccttag cagtttttat tcatagcgtc
    25561 tgcaacgtgc ggaacgaggt cgggtgtacc atgcaatgcc gcctaacaag gcaccaacca
    25621 gggccataaa cagaaatccg accaatgctc ctaaccactt ccccgcggta gatcctaaat
    25681 cactttcaaa tcccgacacg ggggcatcgg ggatccggct catcacccag atgtatcgcg
    25741 ccattgccca gacgaaatag ccacaccatg tatagaaagc ccacaatgaa tcgccacgga
    25801 taatctagac acttccgagc cgttgataat actggttttc atattctgtc ggtgacatct
    25861 gttcgctaga accatgccga cgcttactgt tataaaacat ttcgatgtaa tcaaaaatat
    25921 cactgcgggc ttcttcccgc gttccgtaga tctttttctt tatccgttca cgtttcaaca
    25981 actggaaaaa actttctgca accgcattat catggcagtt accgcgacgg ctcatgctac
    26041 cctccaggcc gtgtgatttc aggaacgact gccactcatg gcttgtgtac tgactgccct
    26101 gatccgaatg aaccagcacc tgtttttcgg gattacgccg ccatacagcc atcagcagtg
    26161 cgttcaggac aatgtccttt gtcatccggg attgcatgga ccagccgata attttgcgtg
    26221 agaacagatc aacaacaacg gcaagataca gccagccttc gtgggtcctg atgtaggtta
    26281 tgtccgttac ccaacgctca tcaggagcat ccggattgaa ctgtcgctgg agcctgttgg
    26341 gtgacacgat actggcctcg cctttacgtg cccgcgggct tcggtatccg acctgagcct
    26401 ttattccgac acgtttcatc agtctccaga ctctgtttac tccgcactgt tgcccgctgt
    26461 cacgcagatc cagatggatt ttgcgataac catagacgca tcccgattcc agccagaact
    26521 gtttaatctg tcctgtcagt ctcaggtctg cctgatggcg ttgtgaatgc ggctgctgaa
    26581 gccaggcgta aaaaccactg ggatgaacat ccagcacccg acagagcagg cgaacaggcc
    26641 agcaacagga gttgtcacgg ataaaggcgt acctcagtcg gacagctttg cgaagtacgc
    26701 cgcggctttt tttaatatgt cccgttcgtc ggtaacccgt ttcagctctt tctggagacg
    26761 gcggatctcg gcctgagcat ctgactgttc tttattagtg gaagaatccg gaccgtactt
    26821 ctttatccag gcataaaggc tgtgggtggt gatatcgaga cgtgttgcaa cgctggcaac
    26881 agaataaccg cgatcaacaa cctgtttgac tgcttcagtt ttaaactctt cgggataacg
    26941 cttaccgctc atgggcacct ctctttaagc catcttaaat gactctgagg tgtctgttaa
    27001 acccgtggcg attcagtgag cgcattctga ccgaccgtga aaatggtgaa aaaggcgata
    27061 attctcaaga tacctcttat gcccgtgtag
//
LOCUS       DAJTUP010000052        26875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTUP010000052 DAJTUP010000000
VERSION     DAJTUP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.052"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO468_002216"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO468_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR6259311.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO468_002217"
     CDS             complement(1614..1937)
                     /locus_tag="OO468_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR6259312.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO468_002218"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO468_002218"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR6259313.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO468_002219"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO468_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR6259314.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO468_002220"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO468_002220"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR6259315.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO468_002221"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO468_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR6259316.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO468_002222"
     CDS             7537..8574
                     /locus_tag="OO468_002222"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR6259317.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVLDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO468_002223"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO468_002223"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR6259318.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO468_002224"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO468_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR6259319.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO468_002225"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO468_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR6259320.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO468_002226"
     CDS             10745..11626
                     /locus_tag="OO468_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6259321.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO468_002227"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO468_002227"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR6259322.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO468_002228"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO468_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR6259323.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO468_002229"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO468_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO468_002230"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO468_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR6259324.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO468_002231"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO468_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR6259325.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO468_002232"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO468_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR6259326.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO468_002233"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO468_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR6259327.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO468_002234"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO468_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR6259328.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO468_002235"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO468_002235"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR6259329.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO468_002236"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO468_002236"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR6259330.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO468_002237"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO468_002237"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR6259331.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO468_002238"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO468_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR6259332.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO468_002239"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO468_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR6259333.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO468_002240"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO468_002240"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR6259334.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO468_002241"
     CDS             24893..25273
                     /locus_tag="OO468_002241"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR6259335.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO468_002242"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO468_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR6259336.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO468_002243"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO468_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR6259337.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..26875
                     /locus_tag="OO468_002244"
                     /pseudo
     CDS             26442..26875
                     /locus_tag="OO468_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6634 a         6684 c         7047 g         6510 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 ctggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcggcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaaccgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcaca
//
LOCUS       DAJTUP010000053        26011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTUP010000053 DAJTUP010000000
VERSION     DAJTUP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.053"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1207)
                     /gene="rhlE"
                     /locus_tag="OO468_002245"
     CDS             complement(<1..1207)
                     /gene="rhlE"
                     /locus_tag="OO468_002245"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR6259338.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNK"
     gene            1436..2107
                     /gene="cecR"
                     /locus_tag="OO468_002246"
     CDS             1436..2107
                     /gene="cecR"
                     /locus_tag="OO468_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR6259339.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2110..3105
                     /gene="hlyD"
                     /locus_tag="OO468_002247"
     CDS             2110..3105
                     /gene="hlyD"
                     /locus_tag="OO468_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR6259340.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3098..4834
                     /locus_tag="OO468_002248"
     CDS             3098..4834
                     /locus_tag="OO468_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6259341.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            4827..5960
                     /locus_tag="OO468_002249"
     CDS             4827..5960
                     /locus_tag="OO468_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6259342.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            5971..7077
                     /locus_tag="OO468_002250"
     CDS             5971..7077
                     /locus_tag="OO468_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6259343.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7039..7449)
                     /locus_tag="OO468_002251"
     CDS             complement(7039..7449)
                     /locus_tag="OO468_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR6259344.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7582..8343
                     /locus_tag="OO468_002252"
     CDS             7582..8343
                     /locus_tag="OO468_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6259345.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8340..9581
                     /gene="clsB"
                     /locus_tag="OO468_002253"
     CDS             8340..9581
                     /gene="clsB"
                     /locus_tag="OO468_002253"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR6259346.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            9581..10537
                     /locus_tag="OO468_002254"
     CDS             9581..10537
                     /locus_tag="OO468_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR6259347.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10573..11286)
                     /locus_tag="OO468_002255"
     CDS             complement(10573..11286)
                     /locus_tag="OO468_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6259348.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(11491..12195)
                     /locus_tag="OO468_002256"
     CDS             complement(11491..12195)
                     /locus_tag="OO468_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR6259349.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(12332..12784)
                     /gene="moaE"
                     /locus_tag="OO468_002257"
     CDS             complement(12332..12784)
                     /gene="moaE"
                     /locus_tag="OO468_002257"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR6259350.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(12786..13031)
                     /gene="moaD"
                     /locus_tag="OO468_002258"
     CDS             complement(12786..13031)
                     /gene="moaD"
                     /locus_tag="OO468_002258"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR6259351.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13024..13509)
                     /gene="moaC"
                     /locus_tag="OO468_002259"
     CDS             complement(13024..13509)
                     /gene="moaC"
                     /locus_tag="OO468_002259"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR6259352.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(13512..14024)
                     /gene="moaB"
                     /locus_tag="OO468_002260"
     CDS             complement(13512..14024)
                     /gene="moaB"
                     /locus_tag="OO468_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR6259353.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14046..15035)
                     /gene="moaA"
                     /locus_tag="OO468_002261"
     CDS             complement(14046..15035)
                     /gene="moaA"
                     /locus_tag="OO468_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR6259354.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15023..15167)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15432..16340
                     /gene="yvcK"
                     /locus_tag="OO468_002262"
     CDS             15432..16340
                     /gene="yvcK"
                     /locus_tag="OO468_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR6259355.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(16532..18553)
                     /gene="uvrB"
                     /locus_tag="OO468_002263"
     CDS             complement(16532..18553)
                     /gene="uvrB"
                     /locus_tag="OO468_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR6259356.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(19132..19809)
                     /gene="bioD"
                     /locus_tag="OO468_002264"
     CDS             complement(19132..19809)
                     /gene="bioD"
                     /locus_tag="OO468_002264"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR6259357.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(19802..20557)
                     /gene="bioC"
                     /locus_tag="OO468_002265"
     CDS             complement(19802..20557)
                     /gene="bioC"
                     /locus_tag="OO468_002265"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR6259358.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(20544..21698)
                     /gene="bioF"
                     /locus_tag="OO468_002266"
     CDS             complement(20544..21698)
                     /gene="bioF"
                     /locus_tag="OO468_002266"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR6259359.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(21695..22735)
                     /gene="bioB"
                     /locus_tag="OO468_002267"
     CDS             complement(21695..22735)
                     /gene="bioB"
                     /locus_tag="OO468_002267"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR6259360.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            22822..24111
                     /gene="bioA"
                     /locus_tag="OO468_002268"
     CDS             22822..24111
                     /gene="bioA"
                     /locus_tag="OO468_002268"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR6259361.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            24170..24646
                     /locus_tag="OO468_002269"
     CDS             24170..24646
                     /locus_tag="OO468_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR6259362.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(25051..25137)
                     /locus_tag="OO468_002270"
                     /pseudo
     CDS             complement(25051..25137)
                     /locus_tag="OO468_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            25229..>26011
                     /locus_tag="OO468_002271"
     CDS             25229..>26011
                     /locus_tag="OO468_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6259363.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTR"
BASE COUNT         6095 a         6983 c         6721 g         6212 t
ORIGIN      
        1 gtttgttcat cagcctgatg ccgccacgtt gctggcgacc gttctggatc ggttcggctt
       61 tgattgacgg gtccggctca tagcccggaa tcgcaatgcg cgggatctct tttttcagca
      121 gtttttcgat atcacgcagc agtttgtgtt catcaacaca caccagcgac aacgcttcac
      181 cggtagcagc cgcacgaccg gtacgcccga tacggtggac ataatcttcc ggtacgtttg
      241 gcagttcata gttgacgacg tgcggcaact cttcgatatc cagaccgcgc gcagcgatat
      301 cggtcgccac cagtacgcga atgtcacccg atttaaaatc agccagcgca cgggtacgcg
      361 caccttgcga tttattcccg tggatcgcca cactacggat gccatcttta ttgagctgct
      421 cagccagatg gttagcgccg tgtttggtac gggtaaacac cagcacctgc tgccagttcc
      481 ctttaccaat catgtgcgac agcaattcgc gtttgcgttt cttatcgaca aagtgaacgt
      541 gctgggtcac ctgatcagac gcggtattgc ggcgtgccac ttcgatttcc agcgggttgt
      601 gcaacagttt ttccgccagg gctttaatat cgtcagagaa ggtcgcagag aacagcaggt
      661 tctggcgttt cgctggcagt tttgttaaca cgcgacggat atcgtggata aagcccatgt
      721 cgagcatgcg gtcagcttca tcgaggacga ggatttcaac ctgatccagc ttcactgcat
      781 tttgatgttc caggtccagc aaacgtcccg gcgttgccac cagcacatca acgccgccgc
      841 gcaacttcat catttgtggg ttaatgctga caccgccaaa caccaccagc gaacgaatgt
      901 tcaggtattt gctgtaatca cggacgtttt cgccaatctg tgccgccagt tcacgggtcg
      961 gggtaagaat gagcgcacgt accggacgac gccctttggc gtgcggctgg cgagtgatca
     1021 ggtgttgcaa cagcggcagc gtaaagcccg ctgttttgcc ggtgccggtc tgggcgctag
     1081 ccatcaggtc gcggccttcc agcacagcag ggatcgcctg ctgctgaata ggggtgggtt
     1141 cacggtaacc ctgctcggca acggcgcgca ggatatcagg gcttaaaccc aaagaatcga
     1201 aagacataac tactccgaac cgccccgacc gttaccggtg tagttttcag ggagatactg
     1261 aaaaggaaaa tgacaaaaac cacaatgtca tgagggagcg gagtgtagca gtttttgtga
     1321 cgcagcgcat aaattatccc tgtgactggc gtagctaaaa ttcttagcca tactggacaa
     1381 ctcccatatg gagtcataat cttaatcaat catttgatta agaatggact gtgcgatgaa
     1441 taatcctgcc atgacaatca agggtgaaca ggcgaaaaaa cagctgattg ctgccgcact
     1501 ggcgcagttt ggtgaatatg gaatgaacgc caccacccgc gagatagccg cccaggccgg
     1561 gcagaatatc gctgccatca cctactactt cggttcgaaa gaagatttgt acctcgcctg
     1621 cgcccagtgg attgccgatt ttattggcga gcagttccgt ccgcatgccg aggaagccga
     1681 acgcttgttc gcccaaccac agcctgatcg ggctgccatc cgtgaactga tccttcgcgc
     1741 ctgcaggaac atgattaagc tgctcaccca ggatgacacc gtcaacctca gcaagtttat
     1801 ctcccgtgag cagctctctc ccacggcagc ctaccacctg gtgcacgaac aggtgattag
     1861 tccgctacac agccacctga cacgcctgat tgccgcctgg accggctgcg acgctaatga
     1921 cacccgcatg atccttcata cccatgcgtt gattggtgag attctggcgt tccgtcttgg
     1981 caaagaaaca attctgttac gtaccggctg gaccgcgttc gatgaagaaa agaccgaact
     2041 gatcaaccag acggtgacct gtcatatcga cctgattttg caaggattat cgcaaaggag
     2101 tttgtagtga tgaaaaaacc tgtcgtgatc ggactggcgg tagtggtact tgccgccgtg
     2161 gttgccggag gctactggtg gtatcaaagc cgccaggata acggcctgac gctgtatggc
     2221 aacgtggata ttcgtacggt gaatcttagt ttccgtgttg gggggcgcgt tgaatcgctg
     2281 gcggtggacg aaggtgatgc tatcaaagcg ggccaggtgc tgggcgaact ggatcacaag
     2341 ccgtatgaga ttgccctgat gcaggcgaaa gcgggcgtct cggtggcaca ggcgcagtat
     2401 gatctgatgc ttgccgggta tcgcgatgaa gaaatcgctc aggccgccgc agcggtgaaa
     2461 caggcgcaag ccgcctatga ctatgcgcag aacttctata accgccagca aggactgtgg
     2521 aaaagccgca ccatttcggc aaatgacctg gaaaatgccc gctcctcgcg cgaccaggca
     2581 caggcaacgc tgaaatcagc acaggataaa ttgcgtcagt accgttccgg taaccgtgaa
     2641 caggacatcg ctcaggcgaa agccagcctc gaacaggcgc aggcgcaact ggcgcaggcg
     2701 gagctgaatt tacaggactc aacgttgata gcaccgtctg atggcacgct gttaacgcgc
     2761 gcggtagagc caggcacggt tctcaatgaa ggtggcacgg tgtttaccgt ttcactaacg
     2821 cgtccggtgt gggtgcgcgc ttatgttgat gaacggaatc ttgaccaggc ccagccgggg
     2881 cgcaaagtgc tgctttatac cgatggtcgc ccggacaagc cgtatcacgg gcagattggt
     2941 ttcgtttcgc cgactgctga atttaccccg aaaaccgtcg aaacaccgga tctgcgtacc
     3001 gacctcgtct atcgcctgcg tattgtggtg accgacgccg atgatgcgtt acgccaggga
     3061 atgccagtga cggtacaatt cggtgacgag gcaggacatg aatgatgccg ttatcacgct
     3121 gaacggcctg gaaaaacgct ttccgggcat ggacaagccc gccgtcgcgc cgctcgattg
     3181 taccattcac gccggttatg tgacggggtt ggtggggccg gacggtgcag gtaaaaccac
     3241 actgatgcgg atgttggcgg gattactgaa acccgacagc ggcagtgcca cggtgattgg
     3301 ctttgatccg atcaaaaacg acggcgcgct gcacgccgtg ctcggctata tgccgcagaa
     3361 atttggtctg tatgaagatc tcacggtgat ggagaacctc aatctgtacg cggatttgcg
     3421 cagcgtcacc ggcgaggcac gtaagcaaac ttttgctcgc ctgctggagt ttacgtctct
     3481 tgggccgttt accggacgcc tggcgggcaa gctctccggt gggatgaaac aaaaactcgg
     3541 cctggcctgt accctggtgg gcgaaccgaa agtgttgctg ctcgatgaac ccggcgtcgg
     3601 cgttgaccct atctcacggc gcgaactgtg gcagatggtg catgagctgg cgggcgaagg
     3661 gatgttaatc ctctggagta cctcgtatct cgacgaagcc gagcagtgcc gtgacgtgtt
     3721 actgatgaac gaaggcgagt tgctgtatca gggagaacca aaagccctga cacaaaccat
     3781 ggccggacgc agctttctga tgaccagtcc acacgagggc aaccgcaaac tgttgcaacg
     3841 cgccttgaaa ctgccgcagg tcagcgatgg catgattcag gggaaatcgg tacgtctgat
     3901 cctcaaaaaa gaggccacac cagacgatat tcgccatgcc gacgggatgc cggaaatcaa
     3961 catcaacgaa actacgccgc gttttgaaga tgcgtttatt gatttgctgg gcggtgccgg
     4021 aacctcagaa tcgccgctgg gtgcaatatt gcatacggta gaaggcacac ccggcgagac
     4081 ggtgatcgaa gcgaaagaac tgaccaagaa atttggtgat tttgccgcca ccgatcacgt
     4141 caactttgcc gttaaacgtg gggagatttt tggtttgctg gggccaaacg gcgcgggtaa
     4201 atcgaccacc tttaagatga tgtgcggttt gctggtgccg acttccggcc aggcgctggt
     4261 gctggggatg gatctgaaag agagttccgg taaagcgcgc cagcatctcg gctatatggc
     4321 gcaaaaattt tcgctctacg gcaacctgac ggtcgaacag aatttacgct ttttctctgg
     4381 tgtgtatggc ttacgcggtc gggcgcagaa cgaaaaaatc tcccgtatga gcgaggcatt
     4441 tggcctgaaa agtattgcct cacacgccac cgatgaactg ccattaggtt ttaaacagcg
     4501 gctggcgctg gcctgttcgc tgatgcatga accggatatt ctctttctcg acgaaccgac
     4561 ttccggcgtt gaccccctca cccgccgtga attttggctg cacatcaaca gcatggtaga
     4621 gaaaggcgtc acggtgatgg tcaccaccca ctttatggat gaagcggaat attgcgaccg
     4681 catcggcctg gtgtaccgtg ggaaattaat cgccagcggt acgccggacg atttgaaagc
     4741 acagtcagct aacgatgagc aacccgatcc caccatggag caagccttta ttcagttgat
     4801 ccacgactgg gataaggagc atagcaatga gtaacccgat cctttcctgg catcgcgtgc
     4861 gggcgctgtg cgtcaaagag acgcggcaga tcgttcgcga tccgagtagc tggctgattg
     4921 cggtagtgat cccactgcta ctgctgttta tttttggtta cggcattaac ctcgactcca
     4981 gcaagctgcg ggtcgggatt ttgctggaac agcgtagcga agcggcgctg gatttcaccc
     5041 acaccatgac cggttcgccc tacatcgacg ccaccatcag cgataaccgt caggaactga
     5101 tcgccaaaat gcagtcgggg aaaattcgcg gtctggtggt tattcccgtg gatttcgccg
     5161 aacagatgga gcgcgccaac gccaccgcac cgattcaggt gatcaccgac ggcagtgagc
     5221 cgaataccgc taactttgtg caggggtatg tcgaagggat ctggcagatc tggcaaatgc
     5281 agcgagcgga ggacaacggg cagacttttg aaccgcttat tgatgtacaa acccgctact
     5341 ggtttaaccc ggcggcgata agccagcact ttattatccc gggtgcggtg accattatca
     5401 tgacggtcat cggcgcgatt ctcacctcgc tggtggtggc gcgagaatgg gaacgcggca
     5461 ccatggaggc tctgctctct acggagatta cccgcacgga actgctgctg tgtaagctga
     5521 tcccttatta ctttctcggg atgctggcga tgttgctgtg tatgctggtg tcagtgttta
     5581 ttctcggcgt gccgtatcgc ggttcgctgc tgattctgtt ttttatctcc agcctgtttt
     5641 tactcagtac cctgggaatg gggctgctga tttccacgat tacccgcaac cagttcaatg
     5701 ccgctcaggt cgccctgaac gccgcttttc tgccgtcgat tatgctttcc ggctttattt
     5761 ttcagatcga cagtatgccc gcggtgatcc gcgcggtgac gtacattatt cccgctcgtt
     5821 atttcgtcag caccctgcaa agcctgttcc tcgccgggaa tattccagtg gtgctggtgg
     5881 taaacgtgct gtttttgatc gcttcggcgg tgatgtttat cggcctgacg tggctgaaaa
     5941 ccaaacgtcg gctggattag ggagaagagc atgtttcatc gcttatggac gttaatccgc
     6001 aaagagttgc agtcgttgct gcgcgaacca caaacccgcg cgattctgat tttacccgtg
     6061 ctaattcagg tgatcctgtt cccgttcgcc gccacgctgg aagtgactaa cgccaccatc
     6121 gccatctacg atgaagataa cggcgagcat tcggtggaac ttactcaacg ttttgcccgc
     6181 gccagcgcct ttacacatgt gctgctgctg aaaagcccac aggagatccg cccgaccatc
     6241 gacacacaaa aggcgttact actggtgcgt ttcccggctg acttctcgcg caaactggat
     6301 accttccaga ccgcgccgtt gcagttgatc ctcgacgggc gtaactccaa cagtgcgcaa
     6361 attgccgcca actacctgca acagatcgtc aaaaattatc agcaggagct gctggaagga
     6421 aaaccgaaac ccaacaacag cgagctggtg gtacgcaact ggtataaccc gaatctcgac
     6481 tacaaatggt ttgtggtgcc gtcgctgatc gccatgatca ccactatcgg cgtaatgatc
     6541 gtcacttcac tttccgtcgc ccgcgaacgt gaacaaggta cgctcgatca gctactggtt
     6601 tcgccgctca ccacctggca gatcttcatc ggcaaagccg taccggcgtt aattgtcgcc
     6661 accttccagg ccaccattgt gctggcgatt ggtatctggg cgtatcaaat ccccttcgcc
     6721 ggatcgctag cgctgttcta ctttacgatg gtgatttacg gtttatcgct ggtgggattc
     6781 ggtctgttga tttcatcact ctgctcgaca caacagcagg cgtttatcgg cgtgtttgtt
     6841 tttatgatgc ccgccattct cctttccggt tacgtttcgc cggtggaaaa catgccagtg
     6901 tggctgcaaa atctgacgtg gattaaccct attcgccact ttacggacat taccaagcag
     6961 atttatttga aggatgcgag tctggatatt gtgtggaata gtttgtggcc gctactggtg
     7021 ataacggcca cgacagggtc agcggcgtac gcgatgttta gacgtaaggt gatgtaactt
     7081 cttatctttc gccagcaaag acactaccgc cgggccagca aggatcgcca gccctgcaat
     7141 tgccagcaaa aagttgtttt ccagtacgcg ggacaacagc cacagcacaa tcagcgctgc
     7201 gccaaaatac caggtcgtgg tcagctcttc cagcacgtct gccacctcac gccagcgttg
     7261 ctcggggtgg cgctgaaaca ccagcatcat cactaccgtg acacaataca gtgcgcataa
     7321 tcccacgccg acgtgaacca gagtcttact catccagccc accaccagca gcgccactac
     7381 cagtaaatgc aacataatct gccagcaact tagacccgtt gcgacacgaa cacgttgttg
     7441 ccacttcatg gcttctctcc tgaagaattt atctctactt actcagagta ccgaacttta
     7501 cggaaaattc cgtaagaccg cacctttttg tgacgccgac tacactattt caatgagata
     7561 agaggcaaag acaggaaaat tatgcccgat caaacacaac aattttcgtt caaggtgctc
     7621 accatcaaca ttcacaaagg ctttaccgcg tttaaccgac gcttcatttt gccggaactt
     7681 cgcgatgccg tgcgtaccgt cagcgccgat attgtttgcc tgcaggaagt gatgggcgcg
     7741 cacgaagttc atccgctgca tgtggaaaac tggcccgata cctcgcacta cgagtttctc
     7801 gccgacacta tgtggagcga ttttgcctac ggtcgcaatg ccgtataccc ggaagggcat
     7861 cacggcaacg ccgtactgtc gcgttatccc attgaacatt atgagaatcg cgatgtttcg
     7921 gtcgatggtg cggaaaagcg cggcgtgctc tactgccgca ttgtgccgcc gatgaccgga
     7981 aaagcgattc atgtgatgtg cgtacatctg ggcctgcgtg aggcgcaccg tcaggcgcag
     8041 cttgcgatgc tcgccgaatg ggtgaatgag ctaccggacg gcgaaccggt attggtggcg
     8101 ggtgatttca atgactggcg gcaaaaagct aatcatccgt taaaagtgca ggccggactg
     8161 gatgagattt ttacccgcgc ccacggacgc ccggcgcgca cgtttccggt gcaatttcct
     8221 ctactacgac tggacaggat ctacgtcaaa aatgccagcg ccagcgcgcc aaccgcgttg
     8281 ccgctgcgga catggcgaca cctttctgat catgcccctt taagtgcgga gattcattta
     8341 tgaaatgtag ctggcgcgaa ggcaataaga tccagttgct ggaaaacggc gagcaatatt
     8401 atcccgcggt gtttaaggcg attggcgagg cacaagaacg catcattctt gaaacgttta
     8461 tctggtttga ggataacgtc ggcaaacagc tgcatgcggc actactggca gcagcgcaac
     8521 gcggggttaa agcggaagtc ttgctggatg gctacggttc gccggatctc agcgatgagt
     8581 ttgtcaatga actgacggca gctggcgtag tgttccgcta ctacgatccc cgccctcgcc
     8641 tttttggtat gcgcaccaat gtgtttcgcc ggatgcatcg caaaattgtg gtgatcgacg
     8701 cgcgtatcgc ctttattggc gggctgaatt actccgccga gcatatgtcc agctacggtc
     8761 cagaggctaa acaggattac gcggtacgcc ttgaagggcc gattgtcgaa gatattctcc
     8821 agtttgagct ggaaaacctg cctggacaga gcgcggcacg acgctggtgg cgacgtcatc
     8881 acaaagcgga agagaaccgc cagccgggag aagcgcaggt attgctggtc tggcgcgata
     8941 acgaagaaca tcgcgatgat attgaacgcc attatctgaa aatgctcact caggcgcagc
     9001 gggaagtgat tatcgccaac gcctacttct tccccggcta tcgattttta cacgccttgc
     9061 gtaaagcggc acggcgcggg gtgcggatca aactgatcat tcagggcgaa ccggatatgc
     9121 cgattgtcag agtcggtgcg cgcttgctgt ataactatct ggttaaaggc ggcgttcagg
     9181 tttttgagta ccgccgccgc ccgctccacg gcaaagtggc attgatggac gatcactggg
     9241 cgacagtagg gtccagtaat ctcgatccgc tcagtttgtc actgaatctc gaagcaaatg
     9301 tcatcatcca cgatcgtcat tttaaccaga cgctgcgcga taatctgaac ggcattattg
     9361 ccgcagattg tcagcaggtg gatgaaacca tgctgcccaa acgcacctgg tggaacctga
     9421 ccaaaagcgt gctggcgttc cactttttac gccacttccc ggcgctggtt ggctggcttc
     9481 cggcacacac accacgtctg acgcaggttg atccccccgc acaaccgaca atggaaacgc
     9541 aggatcgggt agaaactgaa aacacggggg taaacccctg atgagtaaat cacacccgcg
     9601 ctggcgctta gcaaagaaga tcctcacctg gctgtttttt atcgcggtga ttgtgttgct
     9661 ggtggtctac gccaaaaaag tggactggga agaggtctgg aaggtcatcc gcgactacaa
     9721 tcgcgttgcg ctgcttagtg cggtcgggct ggtggtcgtc agctatctga tttacggctg
     9781 ctatgacctg ctcgcccgct tttactgcgg tcacaaactg gcgaagcgcc aggtgatgct
     9841 ggtgtcgttt atctgctacg ccttcaacct gacgctcagt acctgggtcg gcggcattgg
     9901 tatgcgctat cgtttgtact cccgactagg gttaccgggc agcactatta cgcggatttc
     9961 ctcgctcagt attaccacca actggctggg ctatatttta ctggcaggga ttatctttac
    10021 cgcaggcgtg gtggagttgc cggattactg gtatgtcgat caaactacgc tgcgtattct
    10081 cggcattggc ttactgatga ttatcgcggt ttatttgtgg ttttgcgctt tcgcgaagca
    10141 ccgccatatg accatcaaag ggcaaaagct ggtgctgcct tcatggaaat tcgccctcgc
    10201 tcagatgctg atttccagcg ttaactggat ggtaatgggg gcgattatct ggctgttact
    10261 tggtcaaagc gtgaactatt tctttgtact gggcgtgtta ctggttagta gtattgctgg
    10321 cgtcatcgtg catattccgg cggggatcgg tgtgctggaa gcggtgttta tcgcactact
    10381 ggctggggag catacctcga aagggagcat tatcgccgcc ctgctcgctt atcgcgtcct
    10441 gtattacttt atcccgctgc tgctggcgct gatctgttac ctgctactgg aaagccaagc
    10501 gaagaagcta cgggcgaaaa atgaagcggc gatgtgaagt ttagttaagt tctttagtat
    10561 ctgcatttac gactaatgaa aaaaacgcgt atgcctttgc cagacaagcg ttatagctat
    10621 cgcggcaagt atcccaaaga agccgataaa atagtaatag agtgttaatg cacctgtaaa
    10681 agcgcaacga accggactcc acaactcttc actatggcat tttcctgcca gttcaaggag
    10741 cttatttcgt ccatgcgaga taatgcctgg ccataacaca atcattaagc aacttattac
    10801 ccaaatgggc cgttcactca ttaatgccgc attcacgatg attaccaggg ccaaccctgt
    10861 caacgtcatc ataatgataa aacggtgaga accaggatca acattaaata ataaaccggc
    10921 cagcatactg attaaaaaca tcgcgcccgc agtgctgaac acgatgataa tggcatatac
    10981 aggaactaaa cttgcaacga caatcgccac caaagagagt aaaaacagat atattacgcc
    11041 cgcgatggcg gcatttatct tgttgatatc cttacttaag ccatacatac aaagtaataa
    11101 taaccctatg gcgacataca ttggtggtga atcatatttg gttgccgtaa tttcgagata
    11161 cttagctccc caggctagtc ctgcggttgc caccaaacct aaagcggtaa tgagccatat
    11221 accgtttaat atcctggcct cgtgctgaaa atctaattta ttgctgtttt gcgagtatga
    11281 ttccattctt ttagttgtcc ttagaaaaaa cattaatatt caccatcaat aaatttaaaa
    11341 ccacgcatac aaaatacttt tgtatttatg gatacggaaa acgattccgc tcgctcataa
    11401 ggcatatttt atacctggta cttgttaagt gtcgttttta gtaagaaaaa aatcccgaca
    11461 catggccggg atagtgatgg tgaaaaagaa ttaacggcgg ttgccgaaga tccgtaacaa
    11521 catcaggaac aggttgatga agtccagata caaggttaac gcaccaagaa tggaatattt
    11581 gcgcaggttc gacgtgtcgc gggtatcaat ctgctcaccc atatttttca atttctgagt
    11641 gtcatacgcc gtcaatccga caaagacaat cacgccgatg taggtaactg cccacatcaa
    11701 tgcttcgctt ttcagccaga agttgaccag cgatgccagc acaatgccga ttaacgccat
    11761 aaacagcata ttgccaaagc cacttaaatc gcgcttcgtg gtgtaaccgt acaggctcat
    11821 tgcgccgaac atcccggcag taacgacgaa agtactggcg atagaagcag cggtatagac
    11881 aatgaatata ctggaaagcg taagacccgt cagcgccgaa taaagcataa agagcatcgt
    11941 cgttacacct gcgctcagct tttgaatcat cgctgataac acaataacca atgctaattg
    12001 cgcgatgatc agaccgatta aaaagacacg gttagtgaac aacagctcca tcacggccgc
    12061 ggaattagcc gcataccagg caacaaatgc ggtcagcaac aagccaacgg tcatccagcc
    12121 atagacttgc gccatataag tttgcaagcc agcccagggt tgtacgattg aatcagaacg
    12181 tgggaatctg tccatgacga tctcctgaag atataaggaa tatcttaagg atactgcaaa
    12241 atgatgaggc tgtgcatcga cgcagcgtaa acgcatgtac tgagcggtga aattgccgga
    12301 cgcagcggtg ccttatccgg ctaacaaaaa actaccagcg ttttgccgcc tgcagatcgc
    12361 tctcccgggc ttcaacccag cggtcgcctt ccggcgtggc ttcgcgcttc cagaacggtg
    12421 cgcgggtttt gagataatcc ataataaact gcccggcttc aaacgcactg ctgcgatgcg
    12481 cactggtgac accgacaaaa acgatttcat cgcccggcca taattccccg atgcggtgaa
    12541 tcaccgtgac gcgccccagc ggccagcggt tacgcgcttc atcaacaatt tctgccagtg
    12601 ctttttcagt catccccgga tagtgttcga gggttaatgc tttgacgctg tcgcccaggt
    12661 tatggttgcg cacctttccg gtgaaggtga ctaccgcacc gtcttcgtca cgctccgcca
    12721 gccacgggta ctcttctcct acgctgaacg gctgcgggcc gacaacaatt ttggtttctg
    12781 ccatcttaac ctccggttac cggcgggaag aaagctactt cgtcgccgtc agtcagcgga
    12841 tggtcaaaac tcaccagcgt ctggttgacg gcagccagca atttgccatc ttccagcgcc
    12901 agcgcccagc gatcgctctg cgcagccagg tgctggcgta acgcttcaac ggttgggtaa
    12961 tccgcagcca cttcggttgc atctgttccc accaactcgc gcacctgggc gaaaaaaaga
    13021 actttaatca tccgcttcca ccttaaagtc acccgacttg ccgccgctct tcgccagcaa
    13081 acgtaccgga ccaatcacca tatctttttg caccgctttg cacatgtcat aaatggtcag
    13141 cgccgccacg gaggccgcgg ttaacgcttc catttcgaca ccggttttcc cggtcaggcg
    13201 gcataaggtt tctatacgca cccgattgtg ctccggctcg gcctgtaaat tgacttcaac
    13261 tttgctgagc atcagcgggt gacacagcgg gatcagatcc caagtgcgtt ttgccgcctg
    13321 aataccggca atacgcgcag tggcaaatac gtcgcctttg tggtggcgac catcaataat
    13381 catcgccagc gtctcgctgc gcatggtgac aaaggcttcc gcccgcgctt cacgcacggt
    13441 ttccgctttg gcggagacat ccaccatgtg cgcttcgcca gcggcgttga tatgggtcag
    13501 ttgcgacata cttatttctt caaatgtgga tggaaattac acggacgcgt acgggcatcc
    13561 agctgcggcg cgatgatatt ttcccatgcg gtacggcacg ctttggtcga acccggcatg
    13621 gcgaaaatca gcgttttgtt agcgacgccc gctaccgcac gagattgcaa cgtggaagtg
    13681 ccaatctctt caaacgacaa catacggaac acttcaccaa aaccttcaac ctcacggtcg
    13741 aacaacggca gcagcgcttc gggagcctga tcaccttccg tcaggccagt accacccgta
    13801 atcaatacca cttgtacatc gtcgctggcg atccacgcag atacctgagc gcgaatagcg
    13861 tagcggtttt ctttcacaat ggcttcatcg acaacgtgat ggcccgcttc ttgcgccgaa
    13921 tcgcgcagat agtgaccgga ggtatcgtct tcttcaccgc gacgattaga aaccgtaaga
    13981 atagcaatac gggtcgggat aaattcagtg cttacctgac tcatctgatc tctccttttg
    14041 acgttttagc cgccaatgta cgataagttt tgcgtaatac cggtgttgtt ttgatgcagg
    14101 aaatgggtct gtttcttctc ccgcagcgcc gctgaaatac gcgcttccag cgcctgttgc
    14161 tgggtatcgc cttccagcag atcgcgcagg ttaacgccgc cttcaccaaa caggcagaga
    14221 tggagtttac caatggagga aacgcgcagg cggttgcaag tggcgcagaa gtctttttca
    14281 tacggcatga taaggccaat ctctccggcg taatctggat ggcaaaagac ttgcgcggga
    14341 ccgtcgctgc gttgacgtaa ttggtggatc cagccgcgac gcagtagctc gtcacgcaga
    14401 acctgaccag agatgtgatg cttacggaag agctcgctgc cctcgcccgt ttccatcagt
    14461 tcgatgaaac gcagctggat agggcgatgc tggatccagt tcagaaaggt gtcgagctgg
    14521 tgatgattaa catcacgcat cagcacggta ttgactttga ccttctcaaa accggcctca
    14581 aatgcagcat caatccctgc catgacctgg ttgaatttat cctgcccggt aatagcgtga
    14641 aactggcggg cgtccaaact gtcgacactg acgttaatgc cagtaagtcc cgcatcgcgc
    14701 cagttcgcca catcgcgttc cagacggtaa ccattggtgg tgaccgcaat ctggcggata
    14761 gcgtcgtttt cccgcacagc ggcgatgata tcggtaaagt cgcggcgtaa agacggctct
    14821 cctcctgtca ggcgcacttt ttcggtgccc agactggcga aggcgcgcgt aacccggcga
    14881 atttcatcga cggtaagaaa gcctttattg gtgacgccgc tcggtttgta gccatccggc
    14941 aggcagtagg tgcaacgaaa gttacacaca tcggtaatcg acaggcgcaa gtagtaaaac
    15001 ttacgcgcaa atacatcagt cagttgtgaa gccatgtaca cctttccaga tacgggaggc
    15061 gaagtcattt cttccttcgc cctggtggca atctttacga gcattgtcac ggccaaagca
    15121 ccgtatcagt tgacccaggt gcagaggcta gagtgtttag tggttaagcc gatactagcg
    15181 tgtaaatgag atttttacca tccacacttt cgctatgtaa tcatgtatat agcgtcatga
    15241 tcgtgctatt tcgctacaga ataagggaaa atggcggttt tactttgata tacatcatca
    15301 gggaggcata gcgtgcatcg tcctttagag atagaagaaa atagtttgta tcctcaatat
    15361 tggcaggtta attgctgttt ccccgcaatt tgcgctactg tagcgcggat aatctgactc
    15421 caggaaacta tatgcgcaat cgtacgctgg ctgatcttga tcgtgtcgtt gctctcggcg
    15481 gagggcatgg actgggacgc gttctctcat cactttcgtc tttgggttct cgtttaacgg
    15541 gtatcgtcac caccacggat aatggtggct cgacggggcg tattcgccgt tcagaaggcg
    15601 gcattgcttg gggcgatatg cgcaactgcc tcaaccagct gataacggaa ccgaacgtcg
    15661 cctccgcgat gtttgaatac cgttttggcg gcaatggcga actttccggt cataatctcg
    15721 gaaacttgat gttaaaggcg ctggatcacc ttagcgtgcg gcctctggaa gccatcaatt
    15781 taattcgtaa tctgctgaaa gtggatacgc atttgattcc aatgtcagag catcctgttg
    15841 atctgatggc gattgacgat caggggcatg aagtttacgg cgaggtcaat atcgaccagt
    15901 taactacgcc gattcaagag ttattgttaa cgcctaatgt acccgcaacg cgtgaggcgg
    15961 ttcacgctat caatgaagcg gatctcatca ttattgggcc tggcagtttt tataccagcc
    16021 tgatgccaat tctgctgctg aaggaaatcg cccaggcatt acgccgcacg ccagcgccga
    16081 tggtttatat cggcaatctg gggcgtgagt tgagtttacc tgcggctaat ttgaagctgg
    16141 aaagcaagct ggcaattatg gagcagtatg ttggtaaaaa ggtcattgat gcggtcatcg
    16201 tcgggccaaa agtggatgtc tcggcggtga aagagcggat tgtgatccag gaggtactgg
    16261 aggccagcga tattccgtat cgtcatgacc gccagttgtt acataacgcg ctggaaaagg
    16321 cgttacaggc tttaggttaa cggaaatccg aaggaaaatt ccggcttcct attgaagaca
    16381 aagtgcgcgt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta caaaccgcgc
    16441 aaattcaata tattgcggag aaaatgtagg cctgataagc gtagcgcatc agactgtttt
    16501 ccgtttgtca tcagtcttct tcgctatcct gttacgatgc ggcaataaac aactcacgca
    16561 actgatgcaa ctggtcacga atttgtgccg cttcttcgaa ctccagattc tgcgcgtgtt
    16621 gcatcatcag cccttccagt tcatggattt tctgctgcaa cgctttcggc aacatatcca
    16681 tcggcacatt atccggctca acaatcgggc gcgattttcc tctgcccttc gctttggttt
    16741 tggcaatgtt ctgccccagc gccaggatat cgaccacttt cttgttcaag ccttgcggcg
    16801 taatgccgtg ttcctcgttg tacttctgct gtttctcacg gcgacgttcg gtttcgccaa
    16861 tcgctttcgc cattgatgga gtgctcctat cgccgtagag aatcgcttta ccgttaacgt
    16921 tacgtgccgc acgaccaatg gtctggatca acgaacgttc ggaacgcagg aagccttctt
    16981 tgtcagcgtc gaggatcgcc accagcgaca cttccggcat atccagacct tcacgcagta
    17041 agttgatccc taccaatacg tcgaactcac ccagacgcaa gtcgcggata atctccatac
    17101 gctcgacggt gtcgatatct gagtgaagat aacgcacgcg ctcaccgtgt tcttcgagat
    17161 attcggtaag atcttccgcc attcgcttgg tcagtgtggt gaccagcacg cgttcgttaa
    17221 ttgccgctcg ctgacgaatc tccgaaagaa gatcatcaac ctgtgtcgcc accggccgca
    17281 cttcgataat cgggtcaagc aatccggttg gacgcaccac ctgatccacc acatcgccgc
    17341 cggatttttc cagctcgtaa ttacccggcg tcgccgaaac atagatggtt tgcggcgcta
    17401 atgcttcgaa ctcttcaaac ttaagcggac ggttatccag cgctgatggc aggcggaagc
    17461 cgtactccac cagtgtctct ttacgcgccc ggtcaccgcg atacatgccg ccaatttgtg
    17521 gaatggtgac gtgagattca tcgacgacca gcagcccatc ggcaggcagg taatcaaaca
    17581 gcgtcggcgg tggctcaccc ggtccacgac cggagaggaa gcgcgagtag ttttcaatcc
    17641 ccgaacagta gcccagctcg tccatcatct ccagatcaaa ctgggtacgc tgggtcagcc
    17701 gctgctcttc cagcagtttg ttgttttcca acagcacttt gcgtctggcg gccagttctt
    17761 ctttgatctc ctccatcgcc tgtacgatgc gctcgcgcgg tgtgacgtag tgcgttttcg
    17821 ggtagatggt aaaacgtgga atagtggaaa caatctgccc ggtcagcggg tcaaataacg
    17881 acaatcgttc cacttcctcg tcaaacagtt ccacgcgaag tgcaatgtca tccgattctg
    17941 ccgggaagat atctatcacc tcgccacgaa cgcggaaagt accacgctgg aatgcttgat
    18001 cattacgagc gtattgcagc tccgccagtc ggcgcagaat cgcgcgctga tcgataatca
    18061 taccgaccgt gagatggagc atcatcttga gatataaatc aggatcgccc agaccataaa
    18121 tcgcggaaac agacgccact acaatcacat cacgccgctc cagcatcgct ttggtggcgg
    18181 acaaacgcat ctgctcaata tgttcgttaa ccgaggcatc tttctcaatg aaggtgtcgg
    18241 aactcggtac ataggcttcc ggctgatagt agtcgtagta ggagacgaaa tactccaccg
    18301 cgttttccgg gaagaactct ttcatttcgc catacagctg ggccgccagc gttttgttgg
    18361 gcgcaagtac catggttggg cgctgaaggt cagcaatgac attggcaatg gtgaaggttt
    18421 tcccggagcc agtcacgcca agtaacgtct ggtgcgccag gccatcttcc agcccctctt
    18481 cgagacgtcg aatcgcctct ggctgatcgc cagaaggttt aaaagcggaa ttcagtttga
    18541 acggtttact catgagtcgc tacctgaagg agttgggcgg gcaggtatgt aattttactc
    18601 gtcgtactta attatgccaa caaattatac tggataaaaa aacagttcat caccataata
    18661 tttctgatac agcgtaaact ccgctgtcaa tcatgagcaa aatttactct gtggcgagat
    18721 aaaactccag ccttaccggg ttatccccaa agcaacggct tttttaacat ttgtcaagat
    18781 gagtgatgac agttttatgg caagagatgc ctgttcagtg actgccattg catttatcta
    18841 accagttaaa aaataaaaga tatttctttg agccgtcttt aacgccaatt cgcgtgcaag
    18901 ccgcgtattc tctcgcttgc ctcgtgtttt ctaactctaa tacacatggt tatccacagg
    18961 aatagtggat aactgtctcc agcccctatc ccccgccgcc tgggcaattc ccacattcgc
    19021 cgcatgaggc ggaagtaaaa tttttttcgt gattatttta gaatttaatt ggttaaattg
    19081 cagtcaatcg aagacgcgat ctcgctcgca atttaaccaa atacagaatg gctacaacaa
    19141 ggcaaggttt atgtactttc cggttgccgc attttctgga ttttctgcaa gccaggggat
    19201 ctctcccagc agcggcgcgg gaatcatgcg ggtgagcgtg gtcatatatt cagcgtgacg
    19261 ttttcccgga ggcgtaacat cgttcgccac ccaacccgcc agagtcagtc cggcgtgttg
    19321 tattgcctgc gcagtcaaca tcgcgtgatt aatacagccg agtttcacac caactaccag
    19381 tatcaccggc agttgttcct gtgttaccca atctgcaaaa gtgaaagtgt cagaaagcgg
    19441 cgtaaaccag ccgccagcac cttccactaa cacccagtca gcctgttgtt caagcgcgcg
    19501 taatccggcg ctcattacca atgattctat cggcctgccc tcttgggcgc tgatgatgtg
    19561 cggcgaggtg gattctgcga aggtgtaagg atttaccgtt gcgtaatcca gctgcaggct
    19621 gctgttgtgc tgtaacgcca gcgcgtcact attacgtaag ccttccgggg tcttttcgct
    19681 gccagaggcg accggtttat aacctgccgt ccgataacct actgccttcg cggcttgtaa
    19741 aagtgcacaa ctggcgacgg ttttccccac ttcggtatcc gttccggtga caaaataacg
    19801 tttactcacg agcaatcact cccaaaaaaa gatgatacgt cagaggatat cgcccctgtt
    19861 gttgcggcca ggccagttgc aatcgctgca actgcgaacg cgttaatatt cgcgggtcgc
    19921 gcccttcatg aagatgcgta gcaccgatgc ctttcagcga acgcatggca ctgagcgcat
    19981 catcaaacca tagtgtgatg agctgaatat gatgttgata acgcacgccg ttcagcgact
    20041 cttcgatttc atctggcggt aaaaagcgat tagcatgcgg acgctcgtcc accgcctgcc
    20101 gcgcctgatg cagttcgggt aacgatccct gcaccagcgt ggtaaacgcg accacgcctc
    20161 cggggcgcac cacccgatac agctcgcgga gtgccgtgga taaattaccg caccactgca
    20221 ccgcgagatt gctccatgca agatcgaacg tcgcagtcgc taacggcagg gattcgatat
    20281 ctcctgccag ataatgatct gcggcatctt tatggcgggc ttgcgcaagc attggcggcg
    20341 agagatctaa cgccgtcacc tgcgcgtgac gttcccgcca gtggcggctc atccagccag
    20401 gtccacaacc cgcgtccagt acgtgggtgt atttacgctg tggaagcatt gccagtaagg
    20461 cgtcagcact ctggcgctgt agatctgcat gttgctcata gtgtgcggct gcccgaccaa
    20521 atgccgctgc aatggcttgt ttattaaccg ttgccatgca gcacctccag cagacggtcg
    20581 atatcctgca tttcatgcgc agcggttagc gttaagcgca gtcgcgcagt accagcgggt
    20641 acggttggcg ggcgaatcgc cgtgacccag cagccttgct ggcgcagttt ttctgccagt
    20701 tgtaacgcac ggctgttatc accgacgatc aatggctgga tggcgctgca tgaatcagcc
    20761 agcgtaaacg gcaaatcctg tactccggca cgaaaacgcg caatgagtga caccagtttt
    20821 tcgcgccgtg catccccctc atcacggcga atgaccgcca gcgacgcacg taatgcctgc
    20881 gcctgagcgg gcggcatact ggtgctgtag ataaggtggc gggcgaattg cagcagataa
    20941 tccgccaccg tacttgagca aagcaccgca gccccactga cgccaaatcc tttgccaaaa
    21001 gtcactacca gcaattctgg ttttaccttt tgcagccagc agctgccgcg cccctgctcc
    21061 ccgataacgc ccgtgccgtg ggcatcatcg accatcaacc agccattgtg ctgttgcgtt
    21121 acctgctgga tttccgccag tggcgcgcta tcgccgtcca tgctgaacac gccttctgtc
    21181 accaccagtt gctgccccgg acagggggaa gcaagcagtc gcgccagatg agcgacatcg
    21241 ttatgagtaa aacggcgaag ctgcgacggg cttaaactgg cagcctccag caatgaggca
    21301 tggctaagcc ggtcggcaac aatacggtcc tctttcgcca tcatcgcggc aatgactgcc
    21361 tgattcgcgg caaaaccaga gataaacagc agcgcccgcg aatagccaag ccactcggcc
    21421 agttcttctt ccagcgcctg atgcaccacg ctataaccgc tgacgtgacc ggagccgccg
    21481 ctaccgatgc caaattgctc cgccccctgc ttccaggcac ggataatttg cggatgatgg
    21541 cttaaaccta aataatcgtt actggaaaag ttcagatact ggcgatcatc cgccaccagc
    21601 cagcgtccgg ctccttgcgc caccggataa cggcgacgca gggcatcggc agcacgccgc
    21661 gcatcgagcg ccgcgttgat tttctcctgc cagctcataa tgctgccgcg ttgtaatatt
    21721 cgtcggtgtc cggggtcatc agcgcctgtc caagacgttg ctgttgttcg ttatcccctg
    21781 ccagcacggc agtttgctgc ggatttagcc ccagtttgcg gaacagttgc aggtctttat
    21841 cttcttccgg attcggcgtg gtcagcagtt tgcaaccgta gaaaatcgag tttgcgcctg
    21901 ccataaagca catcgcctga gtctgttcgt tcatctgctc gcgtccggca gaaaggcgca
    21961 cgtaagaggt cggcatcatg atccgcgcta cggcaatggt gcgaataaaa tcaaaggcat
    22021 caacgtcatc gttatcggca agcggcgtgc ctttcacctt caccagcatg ttgattggta
    22081 cgctttccgg cggcgtcggc aggtttgcca gttgcagcaa taatccggcg cgatctttta
    22141 ccgtttcgcc taagcccaca atgccacctg agcagacttt gatcccggca tcgcgcactt
    22201 tttccagcgt atcgaggcgt tcctgataag tgcgtgtggt gatgatattg ccgtaaaact
    22261 ccggcgaggt gtccaggttg tggttgtagt aatccagccc ggcgttcgcg aggcgctgcg
    22321 cctgagattc actcaacgtg cccagcgtca tacacgcctc cagccccatc gcttttaccc
    22381 cctgcaccat ttgttccagg tacggcatat cgcgttcgtg gggattcttc catgccgcgc
    22441 ccatacagaa gcgcgtcgat cctgccgctt tcgctttgcg cgccgactcc agcacctgtt
    22501 caacttccat caaccgctcg gcttccagcc cggttttgta gcgcgagctt tgcgggcagt
    22561 atttgcaatc ttccggacaa gctccggtct taatcgacag caacgtgctg acctgcacct
    22621 gacgaggttc gaaatgctgg cgatgcacct gctgcgcttc aaacagcaga tccagcaacg
    22681 gtttttcaaa taattctgtg acttgcgaca atgtccagcg tgggcggtga gccatggggc
    22741 ttctccaaaa cgtgtttttt gttgttaatt cggtgtaggc ttgtaaacct aaatcttttc
    22801 aatttggttt acaagtcgat tatgacaacg gacgatcttg cctttgacca acgccatatc
    22861 tggcacccat acacatccat gacctcccct ctgccggttt atccggtggc gagcgccgaa
    22921 ggttgcgagc taattttgtc tgacggcaga cgcctggttg acggtatgtc gtcctggtgg
    22981 gcggcgatcc acggctacaa tcacccgcag cttaatgcgg cgatgaagac gcaaattgat
    23041 gccatgtcgc atgtgatgtt tggcggtatc acccatgcgc ccgccatcga gctatgccgc
    23101 aaactggtgg caatgacgcc gcaaccgctg gagtgcgttt ttctcgcgga ctccggttcc
    23161 gtagcggtgg aagtggcgat gaaaatggcg ttgcagtact ggcaagccaa aggcgaagcg
    23221 cgccagcgtt ttctgacctt ccgcaatggt tatcatggcg atacctttgg cgcgatgtcg
    23281 gtgtgcgatc cggacaactc aatgcacagc ctgtggaaag gctatctgcc agaaaacttg
    23341 tttgctcccg ccccgcaaag ccgcatggat ggtgaatggg atgaacgcga tatggttggt
    23401 tttgcccgcc tgatggcggc gcatcgtcat gaaatcgcag cggtgatcat tgagccgatt
    23461 gtccagggcg caggcgggat gcgcatgtac catccggaat ggttaaaacg aatccgcaaa
    23521 atgtgcgatc gcgaaggtat cttgctgatt gccgacgaga tcgccaccgg atttggtcgt
    23581 accggcaaac tgtttgcctg tgaatatgca gaaatcgcgc cggacatttt gtgcctcggt
    23641 aaagccttaa ccggcggcac aatgaccctt tccgccacac ttaccacgcg cgaggttgca
    23701 gaaaccatca gtaacggcga agccggctgc tttatgcatg ggccaacttt tatgggcaat
    23761 ccgctggcct gcgcggcagc aaacgccagc ctagcgatta tcgaatccgg cgaatggcag
    23821 cagcaggtgg cggctattga agtgcagctg cgcgagcaac tggcaccagc ccgtgatgcc
    23881 gaaatggttg ccgatgtgcg cgtactgggg gcaatcggtg tggtcgaaac cactcgtccg
    23941 gtgaatatgg cggcgctgca aaaattcttt gtcgaacagg gggtatggat ccggcctttt
    24001 ggcaaactga tttacctgat gccgccctat attattctcc cgcaacagtt gcagcgtctg
    24061 accgcagcgg ttaaccgcgc ggtacaggat gaaacatttt tttgccaata acgggaagtc
    24121 agcgtgaggg tttctggcta cactttctgc aaacaagaaa ggagggttca tgaaactcat
    24181 cagtaacgat ctgcgcgatg gcgataagtt gccgcatcgt catgtcttta acggcatggg
    24241 ttacgatggc gataatattt caccgcatct ggcgtgggat gatgttcctg cgggaacgaa
    24301 aagttttgtt gtcacctgct acgacccgga tgcgccaacc ggctccggct ggtggcactg
    24361 ggtagttgtt aatttacccg ctgatacccg cgtattaccg caagggtttg gctctggtct
    24421 ggtagcaatg ccagacggcg ttttgcagac gcgtaccgat tttggtaaaa ccggatacga
    24481 tggcgcagcg ccgccgaaag gcgaaaccca tcgctacatt tttaccgttc acgcgctgga
    24541 tgtagagcgt attgatgtcg atgaaggagc cagcggcgcg atggtcgggt ttaacgttca
    24601 tttccactct ctggcaagcg catcgattac cgcgatgttt agttaatcac tctgccagat
    24661 ggcgcaatgc catctggtat cacttaaagg tattaaaaac aactttttgt ctttttacct
    24721 tcccgtttcg ctcaagttag tataaaaaag ctgaccgaga aacagtaaaa atcattaata
    24781 tcaatgcatt ataatacatt tagcttaaaa aatagactgc ataatactgc aaaacacaac
    24841 atatccagtc actatgaatc aaccacttag atggtattag tgacctgata cagagcatta
    24901 gcgcaaggtg attttttgtc ctcttgcgct aattttttgt caacgcactg ggttagctga
    24961 atttacaacc atgctcagta tctcgataag cgcagagaaa tgatgctgtg gtgggcagac
    25021 tactggcttg atgaaaaggt agggcgacca accgcgtttt ttcagttcag tcagcacatt
    25081 gagccagaac ttcacccctt cattttcggc cagccacatc cccaacagtt cttcctgttc
    25141 ttaactattt acaggcgcgt ttttttaaag aatcctcact ccatttgcac gaatatatta
    25201 atatataaac aatgaggatc aataataaat gaaacctgcc cacaatcctt cttttttccg
    25261 ctccttttgt ggtttaggat gtatatcccg tttatccgta gaagagcaaa atatcacgga
    25321 ttatcaccgc atctgggata actgggccaa ggaaggtgct gcaacagaag accgaacaca
    25381 ggcagttcga ttactgaaaa tatgtctggc ttttcaagag ccagccctca atttaagttt
    25441 actcagatta cgctctctcc catacctgcc cccgcacata caagaactta acatctctag
    25501 caatgagcta cgctctctgc cagaactccc tccgtcctta actgtactta aagccagcga
    25561 taacagactg agcaggctcc cggctcttcc gcctcacctg gtcgctcttg atgtttcact
    25621 taacagagtt ttaacatgtt tgccttctct tccatcttcc ttgcagtcac tctcagccct
    25681 tctcaatagc ctggagacgc tacctgatct tcccccggct ctacaaaaac tttctgttgg
    25741 caacaaccag cttactgcct taccagaatt accatgtgaa ctacaggaac taagtgcttt
    25801 tgataacaga ttacaagagc taccgcccct tcctcaaaat ctgaggcttt taaacgttgg
    25861 ggaaaaccaa ctacacagac tgcccgaact tccacaacgt ctgcaatcac tatatatccc
    25921 taacaatcag ctgaacacat tgccagacag tatcatgaat ctgcacattt atgcagatgt
    25981 taatatttat aacaatccat tgtcgactcg c
//
LOCUS       DAJTUP010000054        25258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTUP010000054 DAJTUP010000000
VERSION     DAJTUP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.054"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            55..1032
                     /locus_tag="OO468_002272"
     CDS             55..1032
                     /locus_tag="OO468_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR6259364.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(1102..1584)
                     /locus_tag="OO468_002273"
     CDS             complement(1102..1584)
                     /locus_tag="OO468_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR6259365.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            1819..4401
                     /gene="leuS"
                     /locus_tag="OO468_002274"
     CDS             1819..4401
                     /gene="leuS"
                     /locus_tag="OO468_002274"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR6259366.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4416..4997
                     /gene="lptE"
                     /locus_tag="OO468_002275"
     CDS             4416..4997
                     /gene="lptE"
                     /locus_tag="OO468_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR6259367.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            4997..6028
                     /gene="holA"
                     /locus_tag="OO468_002276"
     CDS             4997..6028
                     /gene="holA"
                     /locus_tag="OO468_002276"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR6259368.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6030..6671
                     /gene="nadD"
                     /locus_tag="OO468_002277"
     CDS             6030..6671
                     /gene="nadD"
                     /locus_tag="OO468_002277"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR6259369.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6695..7306
                     /locus_tag="OO468_002278"
     CDS             6695..7306
                     /locus_tag="OO468_002278"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR6259370.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            7566..7883
                     /gene="rsfS"
                     /locus_tag="OO468_002279"
                     /gene_synonym="rsfA"
     CDS             7566..7883
                     /gene="rsfS"
                     /locus_tag="OO468_002279"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR6259371.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7887..8354
                     /gene="rlmH"
                     /locus_tag="OO468_002280"
     CDS             7887..8354
                     /gene="rlmH"
                     /locus_tag="OO468_002280"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR6259372.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8385..10286
                     /gene="mrdA"
                     /locus_tag="OO468_002281"
     CDS             8385..10286
                     /gene="mrdA"
                     /locus_tag="OO468_002281"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR6259373.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10289..11401
                     /gene="mrdB"
                     /locus_tag="OO468_002282"
                     /gene_synonym="rodA"
     CDS             10289..11401
                     /gene="mrdB"
                     /locus_tag="OO468_002282"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR6259374.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11412..12500
                     /gene="rlpA"
                     /locus_tag="OO468_002283"
     CDS             11412..12500
                     /gene="rlpA"
                     /locus_tag="OO468_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR6259375.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12639..13850
                     /gene="dacA"
                     /locus_tag="OO468_002284"
     CDS             12639..13850
                     /gene="dacA"
                     /locus_tag="OO468_002284"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR6259376.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            13961..14224
                     /locus_tag="OO468_002285"
     CDS             13961..14224
                     /locus_tag="OO468_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR6259377.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14325..14966
                     /gene="lipB"
                     /locus_tag="OO468_002286"
     CDS             14325..14966
                     /gene="lipB"
                     /locus_tag="OO468_002286"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR6259378.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15225..16178
                     /locus_tag="OO468_002287"
     CDS             15225..16178
                     /locus_tag="OO468_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6259379.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            16387..17352
                     /gene="lipA"
                     /locus_tag="OO468_002288"
     CDS             16387..17352
                     /gene="lipA"
                     /locus_tag="OO468_002288"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR6259380.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17453..17656)
                     /gene="tatE"
                     /locus_tag="OO468_002289"
     CDS             complement(17453..17656)
                     /gene="tatE"
                     /locus_tag="OO468_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR6259381.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17785..18573)
                     /locus_tag="OO468_002290"
     CDS             complement(17785..18573)
                     /locus_tag="OO468_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR6259382.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            18666..19049
                     /gene="crcB"
                     /locus_tag="OO468_002291"
     CDS             18666..19049
                     /gene="crcB"
                     /locus_tag="OO468_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR6259383.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(19103..19312)
                     /gene="cspE"
                     /locus_tag="OO468_002292"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19103..19312)
                     /gene="cspE"
                     /locus_tag="OO468_002292"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6259384.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19487..20047)
                     /gene="pagP"
                     /locus_tag="OO468_002293"
     CDS             complement(19487..20047)
                     /gene="pagP"
                     /locus_tag="OO468_002293"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR6259385.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            20636..22021
                     /gene="dcuC"
                     /locus_tag="OO468_002294"
     CDS             20636..22021
                     /gene="dcuC"
                     /locus_tag="OO468_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR6259386.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(22062..22403)
                     /locus_tag="OO468_002295"
                     /pseudo
     CDS             complement(22062..22403)
                     /locus_tag="OO468_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(22741..23250)
                     /locus_tag="OO468_002296"
     CDS             complement(22741..23250)
                     /locus_tag="OO468_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6259387.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            23413..23658
                     /locus_tag="OO468_002297"
     CDS             23413..23658
                     /locus_tag="OO468_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6259388.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            23633..>25258
                     /locus_tag="OO468_002298"
     CDS             23633..>25258
                     /locus_tag="OO468_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HCR6259389.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAEGVAMFNART
                     GRETEMCGGKLSFDDVFEREYARTIVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGG
                     SLKGAKNVYYNMALMNAGVKKVVVRFDPQQLHST"
BASE COUNT         6400 a         6210 c         6302 g         6346 t
ORIGIN      
        1 aatggaacac ataaaaatat aatatatttt atctacaacc ataaggaaat gataatgatt
       61 tttacgtcaa gttgctgcga taatttatca atagatgaga ttatcgaacg tgctgaaaaa
      121 ggcgattgcg aggctcaata tattgttggg ttttattata atcgcgatag cgcaattgac
      181 tctccggacg acgaaaaggc cttttactgg ctgaagctgg ccgctgagca aggtcattgt
      241 gaagcacagt attccttagg gcaaaagtat accgaggata aaagccgtca taaagataat
      301 gagcaagcca tcttctggct gaaaaaagct gccctacaag gccatacttt cgcttccaac
      361 gcccttggct ggatactgga tcgtggagaa gaccccaact ataaagaagc ggttacctgg
      421 tatcagatag ccgcggagag cggaatgtct tatgcgcaaa ataatcttgg atggatgtac
      481 agaaatggca acggagtcgc acaagactat acgctggcat ttttctggta caaacaagct
      541 gcattacaag gccatagtta cgcacaagac aatctggccg atctttataa agacggagaa
      601 ggcgttgctc aaaacaagac actcgccgca ttctggtatt tgaaaagcgc acagcagggt
      661 aatcggcacg cccagtttca aattgcatgg gattataacg ctggcgaagg ggtggaccag
      721 gactataagc aagcgatgta ctggtatctg aaggctgccg ctcaggaaag cgtcggcgct
      781 tacgtcaaca tcggttatat gtataaacac ggacaaggcg ttgagaaaga ttatcaggct
      841 gcctttgaat ggtttacgaa agccgctgaa tgcaatgacg ccactgcctg gtataacctg
      901 gccattatgt atcactacgg agaaggaaga cctgtcgatc tccgacaggc tctcgacctg
      961 tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc aggaaattcg tgagactgaa
     1021 gctttactgt agaaataaaa acaagggccg gggaatttca ttctgcccat attctcttta
     1081 ttgctgtgcg aaatcagact attacggctc aaacgggcgt ctctggaact ggtcataacc
     1141 acatttcggg catagcgtca gcacttccgg tgtgtagatc gggagatgga agtgacattt
     1201 ctcgcagacc agatttccca gcccgaccac ttctccgctg tgataaaccc catgatgatt
     1261 gaggtcctgg aaaatttcgc gccattcaag ctgcgtttta tcggtgatgt ctgccagctc
     1321 ctgccacaag ctttctttaa tcacccgcat aaagacgctg tcagattctt ctttcaggct
     1381 ctcttcatag ctcatggcga actcttccag gtcacgtctg acagctcgcg tcagctcatc
     1441 gatctcggtt cgcgttaact cccctgtttt tattacgcgc tcgcgcgcct gttccaccag
     1501 tgcgtcgata tcacgttcgc cattgcgcag gcgttcgttc agtgacgcga ccagttcacg
     1561 gtaatattga gcaaccttgt tcatcgtttc atctcccggg tcgttaactg actataataa
     1621 tagacgttct ttgttccttg ctttgctaat acgcccacaa actctgtaaa ttaatgtacg
     1681 aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg ctgttttgac gcggacgttt
     1741 gggctatgct atgcggatct gaaaaaccac ctcaacgcta catttgtagc cgtattgaaa
     1801 acaggaccac tggctgccat gcaagagcaa taccgcccgg aagagataga atccaaagta
     1861 cagcttcatt gggatgagaa gcgcacattt gaagtaaccg aagacgagag caaagagaag
     1921 tattactgcc tgtctatgct tccctatcct tctggtcgac tacacatggg ccacgtacgt
     1981 aactacacca tcggtgacgt gatcgcccgt taccagcgca tgctgggcaa aaacgttctg
     2041 cagccgatcg gctgggacgc gtttggtctg cctgcggaag gcgcggcggt gaaaaacaac
     2101 accgctccgg caccgtggac gtacgacaac atcgcgtata tgaaaaacca gctcaaaatg
     2161 ctgggctttg gttatgactg gagccgcgag ctggcaacct gtacgccgga atactaccgt
     2221 tgggaacaga aattcttcac cgagctgtat aaaaaaggcc tggtatataa aaagacttct
     2281 gcggtcaact ggtgcccgaa cgaccagacc gtactggcga acgaacaagt tatcgacggc
     2341 tgctgctggc gctgcgatac caaagttgaa cgtaaagaga tcccgcagtg gtttatcaaa
     2401 atcactgctt acgctgacga gctgctcaac gatctggata aactggatca ctggccagac
     2461 accgttaaaa ccatgcagcg taactggatc ggtcgttccg aaggcgtgga gatcaccttc
     2521 aacgttaacg actatgacaa cacgctgacc gtttacacta cccgcccgga cacctttatg
     2581 ggttgtacct acctggcggt agctgcgggt catccgctgg cgcagaaagc ggcggaaaat
     2641 aatcctgaac tggcggcctt tattgacgaa tgccgtaaca ccaaagttgc cgaagctgaa
     2701 atggcgacga tggagaaaaa aggcgtcgat actggcttta aagctgttca cccattaacg
     2761 ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga tggagtacgg cacgggcgca
     2821 gttatggcgg ttccgggtca cgaccagcgc gactacgagt ttgcctctaa atacggcctg
     2881 aacatcaaac cggttatcct ggcggctgac ggctctgagc cagatctttc tcagcaagcc
     2941 ctgactgaaa aaggcgtgct gttcaactct ggcgagttca acggtcttga ccatgaagcg
     3001 gccttcaacg ccatcgccga taaactgact gcgatgggcg ttggcgagcg taaagtgaac
     3061 taccgcctgc gcgactgggg tgtttctcgc cagcgttact ggggcgcgcc gattccgatg
     3121 gtgacgctgg aagacggtac cgtaatgcca accccggacg accagctgcc ggtgatcctg
     3181 ccggaagatg tggtgatgga cggcattacc agcccgatta aagcagatcc ggagtgggca
     3241 aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg acactttcga cacctttatg
     3301 gagtcctcct ggtactatgc gcgctacact tgcccagagt acaaagaagg tatgctggat
     3361 tccgaagcgg ctaactactg gctgccggtg gatatctaca ttggtggtat cgaacacgcc
     3421 attatgcacc tgctctactt ccgcttcttc cacaaactga tgcgtgatgc aggcatggtg
     3481 aactctgacg aaccagcgaa acagttgctg tgtcagggta tggtgctggc agatgccttc
     3541 tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc cggttgatgc tatcgttgaa
     3601 cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg caggccatga actggtttat
     3661 accggcatga gcaaaatgtc caagtcgaag aacaacggta tcgacccgca ggtgatggtt
     3721 gaacgttacg gcgcggacac cgttcgtctg tttatgatgt ttgcttctcc ggctgatatg
     3781 actctcgaat ggcaggaatc cggtgtggaa ggggctaacc gcttcctgaa acgtgtctgg
     3841 aaactggtgt acgagcacac agcaaaaggt gatgttgcgg cactgaacgt tgatgcgctg
     3901 actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa ctatcgctaa agtgaccgat
     3961 gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg cgattatgga gctgatgaac
     4021 aaactggcga aagcaccgac cgatggcgag caggatcgcg ctctgatgca ggaagcactg
     4081 caggccgttg tccgtatgct caacccgttc accccgcaca tctgcttcac gctgtggcag
     4141 gaactcaaag gcgaaggcga tatcgacaac gcgccgtggc cggttgctga cgaaaaagcg
     4201 atggtggaag actccacgct ggtcgtggtg caggttaacg gtaaagtccg tgccaaaatc
     4261 accgtcccgg tggacgcaac ggaagaacag gttcgcgaac gtgctggcca ggaacatctg
     4321 gtagcaaaat atcttgatgg cgttactgta cgtaaagtga tttacgtacc aggtaaactc
     4381 ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg atatctggca acactgttgt
     4441 tatctctggc ggtgttaatc accgccgggt gtggctggca tctgcgtgac accacgcagg
     4501 ttccttccac tatgaaggtc atgatcctgg actcaggcga tccgaacggg ccattaagcc
     4561 gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt gcttgataaa gaaaccacgc
     4621 gtaaggacgt tccatccctg cgtttgggta aagtgagcat cgcgaaagat accgcatcgg
     4681 tattccgtaa cggtcaaaca gcagagtatc agatgatcat gacggtgaat gcgaccgtgt
     4741 tgatccccgg ccgtgatatc tacccgatta gcgccaaagt cttccgttca ttcttcgata
     4801 acccgcaaat ggcgttagcg aaagataacg aacaagacat gatcgtaaaa gagatgtacg
     4861 accgtgctgc cgaacagctg attcgtaagc tgccaagcat ccgtgctgcg gatattcgtt
     4921 ccgacgaaga acagacgtcg accacaacgg atactccggc aacgcctgca cgcgtctcca
     4981 ccatgctggg taactgatga ttcggttgta cccggaacaa ctccgcgcgc agctcaatga
     5041 agggctgcgc gcggcgtatc ttttacttgg taacgatcct ctgttattgc aggaaagcca
     5101 ggacgctgtt cgccaggtag ctgcggcaca aggattcgaa gaacaccaca ctttttccat
     5161 tgatcccaac actgactgga atgcgatctt ttcgttatgc caggctatga gtctgtttgc
     5221 cagtcgacaa acgctattgc tgttgttacc agaaaacgga ccgaatgcgg cgatcaatga
     5281 acaacttctc acactcaccg gacttctgca tgacgacctg ctattgatcg tccgcggtaa
     5341 taaattaagc aaagcgcaag aaaatgccgc ctggtttact gctcttgcga atcgcagcgt
     5401 gcaggtgacc tgtcagacac cggagcaggc tcagcttccc cgctgggttg ctgtgcgcgc
     5461 aaaacagctc aacttagaac tggatgacgc ggcgaatcag gtgctctgct actgttatga
     5521 aggtaacctg ctggcgctgg ctcaggcact ggagcgttta tcgctgctct ggccagacgg
     5581 caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat gccgcgcatt tcaccccttt
     5641 tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc gcgttgcata ttcttcagca
     5701 actgcgtctg gaaggcagcg agccggttat tttgttgcgc acattacaac gtgaactgtt
     5761 gttactggtt aacctgaaac gccagtctgc ccatacgcca ctgcgtgcgt tgtttgataa
     5821 gcatcgggta tggcagaacc gccggggcat gatgggcgag gcgttaaatc gcttaagtca
     5881 gccgcagtta cgtcaggccg tgcaactcct gacacgaacg gaactcaccc tcaaacaaga
     5941 ttacggtcag tcagtgtggg cagagctgga agggttatct cttctgttgt gccataaacc
     6001 cctggcggac gtatttatcg acggttgata tgaaatcttt acaggctctg tttggcggca
     6061 cctttgatcc ggtgcactat ggtcatctaa aacccgtgga aacgctggcg aatttgattg
     6121 gtctgacgcg ggtcacaatc atccctaata atgttcctcc gcatcgtccc cagccggaag
     6181 cgaacagcgt gcagcgtaaa cacatgcttg aactggcgat tgccgacaag ccattattta
     6241 ctcttgatga acgcgagcta aagcgcaatg ccccctctta cactgcgcaa acactgaaag
     6301 agtggcggca ggaacaagga ccggacgtgc cgctggcgtt tattattggt caggattcat
     6361 tgctgacctt tccgacctgg tacgaatacg aaacgatact cgacaatgca catttgatcg
     6421 tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc gcaataccag caatggctgg
     6481 aagatcattt gacacataac ccggaagatc ttcaccttca gcctgccggt aaaatttatc
     6541 tggctgaaac gccgtggttt aacatctcgg cgaccatcat ccgcgaacgt ttgcaaaacg
     6601 gtgaatcgtg tgaggattta ttgccggaac cggtattgac ttacattaac caacaaggct
     6661 tgtatcgctg atacctgtcg ttacggaggg catgatgcga ctgtggttaa ttcgtcatgg
     6721 cgaaacacaa gcgaatgtcg atggtctgta cagcggtcat gcgcccacac ccctgaccgc
     6781 acgcggtatt gagcaagcgc aaaatctgca tacactgcta gatgatgttt cctttgactt
     6841 ggttttatgc agtgaactgg aacgggcaca gcataccgcg cgactggttc tcagtgaccg
     6901 ccagctcccc gttcatatca tccctgaact caacgaaatg ttttttggcg actgggagat
     6961 gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac tatagcgcgt ggtgcaatga
     7021 ctggcagcat gctatcccca cgaacggtga aggatttcag gcattttcgc aacgtgtgga
     7081 acgctttatc gcaaggctta gtgaatatca gcactatcag aatattttaa tcgtcagcca
     7141 tcagggcgta ctgagtctgt taattgctcg tttaattggc atgcctgccg agtctatgtg
     7201 gcattttcgc gttgaccagg ggtgttggag cgccattgat atcaaccaaa aattcgcgac
     7261 gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaac gcatgacttt tctgtttttt
     7321 tacgggtaag ccgcaacgac cattgacagc cccgggcagg ctgatattct ccgcagccag
     7381 actttttccg ccagacacga ctttgtagaa attgttttac aaaaatggcg atgcaatctg
     7441 cggcgcgggg tgggatgata gcccactttc gaaagccgat tcggcgacaa ttgtcccgaa
     7501 atcgcctctg gttcaggtat actgacagac catttttatc tatttgattc acccaggggg
     7561 aaaacttgca gggtaaagca ctccaggatt ttgttatcga caaaattgat gacctcaaag
     7621 gtcaggacat catcgcctta gacgttcaag gcaaatccag catcaccgac tgcatgatca
     7681 tctgtacggg tacgtccagc cgtcatgtta tgtccattgc tgaccacgtt gtgcaggagt
     7741 ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga aaacagcgcc gactggattg
     7801 tcgtggattt gggcgatgtg attgtccatg tcatgcagga agagagccgt cgcctgtatg
     7861 aactggaaaa actctggagt taatgcgtga agctgcaact tgtcgccgtg ggaacgaaaa
     7921 tgccggactg ggtacaaacc ggttttaccg agtacctgcg tcgttttccg aaagatatgc
     7981 ccttcgagct gattgaaatt ccggccggca aacgcggcaa gaatgcggac atcaagcgca
     8041 tactcgacaa agagggtgag cagatgttgg cggccgcagg caaaaaccgc attgtcaccc
     8101 tcgatattcc aggcaagccc tgggatacgc cgcagttagc cgctgagctg gaacgctgga
     8161 agctggatgg tcgcgacgta agtctactga ttggcgggcc tgaagggttg tcgcctgcct
     8221 gtaaagcggc ggctgagcag agctggtcgc tgtcggcgct taccctcccc catccgctgg
     8281 ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag catcaccacc aaccatcctt
     8341 atcaccgtga gtgataaggg agctttgagt agaaaacgca gcggatgaaa ctacagaact
     8401 cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg ccgggcgctg gtcgcctttt
     8461 tggggatttt gctgctgacc ggcgtgctta tcgccaacct gtataatctg caaattgttc
     8521 gctttaccga ctaccagacc cgctctaatg aaaaccgcat taagctggtg cctatcgcgc
     8581 ccagccgcgg cattatctac gaccgtaacg gtatccctct ggccctcaac cgcactatct
     8641 accagataga aatgatgccg gagaaagtcg ataacgtgca gcaaacgctg gacgctttgc
     8701 gcagcgtggt agatctgacc gatgacgata ttgctgcatt ccgaaaagag cgcgcacgtt
     8761 cacaccgttt cacctctatt ccggtgaaaa ctaacctaac cgaagtacaa gtagctcgct
     8821 ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa aggctataaa cgtcgttact
     8881 atccttacgg ttcggcgttg acccacgtca tcggctatgt gtcgaaaatc aacgataaag
     8941 acgtcgaacg cctgaataat gacggcaaac tggccaacta tgcggcaacg catgatattg
     9001 gtaagctggg cattgagcgt tactatgaag acgtgctgca cggtcagacc ggttatgaag
     9061 aggttgaagt taacaaccgt gggcgtgtta tccgccagtt aaaagaagta ccaccgcaag
     9121 ccggacacga tatttacctg acgctggatc tcaaactcca gcaatatatt gaaacgctgc
     9181 tggcgggtag ccgcgcagct gtggttgtta ccgaaccgcg tacaggtggg gtgctggcgc
     9241 tggtttccac gcctagttat gacccaaacc tgtttgttga cggtatctcc agcaaagatt
     9301 attccgcctt gctgaacgac ccgaatacac cgctggtgaa ccgcgccaca cagggggttt
     9361 atcctcccgc gtctacagtt aaaccctatg tggcggtttc ggcattaagc gccggggtga
     9421 tcacgcgcaa tacgacgctg tttgacccag gctggtggca actaccaggg tcggaaaaac
     9481 gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa tgtcacaaga tcgctggaag
     9541 aatctgcgga taccttcttc tatcaggtgg cctacgatat ggggatcgat cgcctctccg
     9601 aatggatggg taaattcggt tatggtcatt acaccggtat cgacctggcg gaagaacgtt
     9661 ccggcaacat gcctacccgc gaatggaaac agaaacgctt taaaaaaccg tggtatcagg
     9721 gtgacaccat tccggttggt atcggccagg gttactggac agcgacccca atccagatga
     9781 gtaaggcact gatgatcctg attaatgacg gtatcgtgaa ggttccgcat ttgctgatga
     9841 gcaccgccga agacggcaaa caggtgccat gggtacaacc gcatgaaccg cccgtcggcg
     9901 atattcattc cggttactgg gagctggcga aagacggtat gtacggtgtt gctaaccgcc
     9961 ctaatggtac ggcgaataaa tactttgcca gcgcaccgta caaaattgcg gcgaaatctg
    10021 gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta taatgcgcac aaaattgccg
    10081 agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc atacaacaat ccgcaagtgg
    10141 ctgtcgccat gattctggag aacggcggcg cgggtccggc ggttggtacg ctgatgcgcc
    10201 agatcctcga ccacattatg ctgggtgata acaacaccga tctgcctgcg gaaaatccag
    10261 cggttgccgc agcggaggac cattaatcat gacggataat ccgaataaaa aaacattctg
    10321 ggataaagtc catctcgatc ccacaatgct gctgatttta ctggcattgc tggtttacag
    10381 cgccctggtt atctggagcg ccagcggtca ggatattggc atgatggagc gtaaaatcgg
    10441 ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg caaattcctc cacgcgttta
    10501 tgaaggctgg gccccctatc tctatatcat ctgtattatt ttgctggtgg cggtagatgc
    10561 tttcggagcc atctctaaag gcgctcaacg ctggctggac ctcggtattg ttcgttttca
    10621 gccgtcggaa attgccaaaa tagccgtacc actgatggtt gcgcgcttta tcaaccgcga
    10681 cgtttgcccg ccatcgttga agaatactgg catcgcgctg gtgctgatat ttatgcccac
    10741 gctgctggtg gctgcacagc ctgacctggg aacatcaatc ctcgttgcgc tttccggtct
    10801 gtttgtactg ttcctctctg gccttagctg gcgtctgatt ggcgtcgcag tagtgctggt
    10861 agcggcgttc attccgattc tgtggttctt cctgatgcat gattaccagc gccagcgcgt
    10921 aatgatgctc ctggacccgg aatcagaccc actcggcgcg ggctatcaca ttattcagtc
    10981 taaaattgct attggctccg gcggattacg cggcaaaggc tggctgcacg gcactcagtc
    11041 acagcttgaa tttctccccg aacgccatac tgactttatc ttcgcggtac tggcggaaga
    11101 gctgggatta gtgggcattc tgattctgct cgctctctac attctgctga tcatgcgcgg
    11161 gctgtggata gccgccagag cgcaaaccac ctttggtcgc gtcatggctg gtggcttaat
    11221 gctgatatta ttcgtttatg tcttcgtaaa tattggtatg gtaagcggta ttctgccggt
    11281 tgtaggggtt ccgctcccac tggtcagtta tggaggatcg gcgctaattg tgctgatggc
    11341 tgggttcggg attgtaatgt caatccacac ccacaggaaa atgttgtcga aaagcgtgta
    11401 agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat cgcggcagga atgctcgcgg
    11461 catgtacaag cgatgatggt cagcaacaga cggtaagtgt accgcagcct gcggtatgta
    11521 acggccccat agttgaaatt agcggggcgg acccgcgttt cgaaccactg aacgcgacgg
    11581 caaatcagga ttaccagcgc gacggtaaaa gctacaaaat cgtgcaggat ccgtctcgat
    11641 ttagccaggc gggactggcg gcaatctatg atgccgaacc aggcagtaac ctgacggcct
    11701 ctggcgaagc tttcgatccg acaaagctga cggcggccca tccaacgctt ccgatcccca
    11761 gctacgccag aatcactaac ctggctaacg ggcgaatgat cgtggtgcgc attaatgatc
    11821 gcggccctta cggcaacgac cgcgttattt cgctttctcg cgcggcagct gaccgtctta
    11881 acacgtcaaa caacaccaaa gttcgtatcg atccgattat tgtcgcccag gatggttcgc
    11941 tttctggtcc tggtatggct tgtaccacag tcgccaaaca gacttacgcc ctgcctgcac
    12001 ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc tggcccgcag ggtgacattc
    12061 ttccggtcag taattcgacg ctaaaaagcg aagatccgac cggcgcgccg gtaaccagta
    12121 gcggtttcct cggcgctcca acgaccttag cgcctggtgt actggaaggc agcgaaccga
    12181 cgcctgcgcc acagcccgtt gttacagctt cgtcgacaac gcctgcaacc tcgcctgcaa
    12241 tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa ctttatggtg caagtcgggg
    12301 ccgtaagcga tcaggctcgt gcgcaacagt accaacagca actgggacag aagttcggcg
    12361 tccccggtcg cgtaactcaa aatggcgcgg tctggcggat ccagcttggc ccattcgcca
    12421 gcaaagccga agccagtacc ttgcagcaac gtttgcaaac cgaagcccaa ttacagtcat
    12481 ttattactac cgcgcagtag cgtaaagcag gcatctgatg tgtcaatttc tgtaaatgac
    12541 gttaacaaag tcacgcggaa agtcagatgc ctgccggtag tgcatttgct atagtagggc
    12601 acttttttaa ttccatcacg gatgtcgtag ttcagaccat gaataccatt ttttccgctc
    12661 gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac agcctttatc tctgctgcac
    12721 atgccgatga cctgaatatc aaaactatga tcccgggtgt accgcagatc gatgcggagt
    12781 cctacatcct gattgactat aactccggca aagtgctcgc cgaacagaac gcagatgtcc
    12841 gccgcgatcc tgccagcctg accaaaatga tgaccagtta cgttatcggc caggcaatga
    12901 aagccggtaa atttaaagaa actgatttag tcactatcgg caacgacgca tgggccaccg
    12961 gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa accgggcatg caggttccgg
    13021 tttctcagct gatccgcggt attaacctgc aatcgggtaa cgatgcttgt gtcgccatgg
    13081 ccgattttgc cgctggtagc caggacgctt ttgttggctt gatgaacagc tacgttaacg
    13141 cactgggcct gaaaaatacc cacttccaga cggtacatgg tctggatgct gatggtcagt
    13201 acagctccgc gcgagatatg gcgctgatcg gccaggcatt gatccgtgac gtaccgaatg
    13261 aatactcgat ctataaagaa aaagaattta cgtttaacgg tattcgccag ctgaaccgta
    13321 acggcctgtt atgggataac agcctgaatg tcgacggcat caaaaccgga cacactgaca
    13381 aagcaggtta caatcttgtt gcttctgcga ctgaaggcca gatgcgcttg atttctgcgg
    13441 tgatgggtgg acgtactttt aaaggccgtg aagccgaaag taaaaaactg ctaacctggg
    13501 gcttccgttt cttcgaaacc gttaacccac tgaaagtagg taaagagttc gcctctgaac
    13561 cggtttggtt tagtgattct gatcgcgctt cgttaggggt tgataaagac gtgtacctga
    13621 ccattccgcg tggtcgcatg aaagatctga aagccagcta tgtgctgaac agcagtgaat
    13681 tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat caacttccag cttgatggca
    13741 aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat cccggaaggt aacttcttcg
    13801 gcaaaatcat tgattacatt aaattaatgt tccatcactg gtttggttaa aaattgaaca
    13861 cttgaaagtg taatttccgt ccccatatac taagcatcag taaaaaaact cccgccttct
    13921 ggcgggagtt gctatttaat tacgttacgc cggagctgac atgaaaacca aacttaacga
    13981 actgcttgaa ttccctactc cttttactta caaagttatg gggcaggcgt tacctgagct
    14041 ggttgatcag gtggttgaag tggtacagcg ccatgcgcca ggtgactaca ccccaacggt
    14101 aaaaccaagc agcaaaggca actaccactc ggtatctatc actatcaacg ccactcatat
    14161 cgagcaggtt gaaacactgt atgaagaact gggcaaaatc gatattgtcc gcatggttct
    14221 gtaactcgct tctccgttac ccggctcctg gtcgggtaac tcccctctcc tcgctgtgat
    14281 atacttttcc cccactttta ctcattctcc acggagatgc cgttttgtat caggataaaa
    14341 ttcttgtccg ccagctcggt cttcagcctt acgagccaat ctcccgggct atgcatgaat
    14401 tcaccgatac ccgcgataat agtacccttg atgaaatctg gctggtcgag cactatccgg
    14461 tattcaccca aggtcaggca ggaaaagcgg agcacatttt aatgccgggt gatattccgg
    14521 tgatccagag cgatcgcggt gggcaggtga cttatcacgg gccgggacaa caggtgatgt
    14581 atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg tgaactggtg accttgcttg
    14641 agcaaacagt ggtgaatacc ctggctgaac tgggtataga agcgcatcct cgggctgacg
    14701 cgcctggtgt ctatgtcggg gaaaagaaaa tttgctcact gggtttacga attcgacgcg
    14761 gttgttcatt ccacggtctg gcattaaacg tcaatatgga tctttcacca tttttacgta
    14821 ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat atcacaatgg aaacccgaag
    14881 cgacgactaa taatattgct ccacgtttac tggaaaatat tttagcgcta ctaaacaatc
    14941 cggacttcga atatattacc gcttaattcc atacatcaat ggcccaattt acactgggtc
    15001 attactcata atacctgcta atgctttaca tctccttcgc gtttgtatat tttctttatc
    15061 acttgcagaa atgcggtgcc acgccttgct ccgtaactca ccccattgat taacctattg
    15121 aaatagttga ttatggtgag caaaagcgtt ccgttccaat tagacataag attattgtaa
    15181 ttttttaaat attatttttc catgaataaa attggagtaa gtgcgtggat agtaataatc
    15241 aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa accacaaata tttacaacct
    15301 tacgaaatat tgatcttaac cttctgacta tttttgaagc tgtatatgta cataaaggga
    15361 tcgttaatgc agcgaaagtg cttaatctga ccccctcggc aatcagtcag tctattcaga
    15421 aactgcgcgt tatattccct gacccattgt ttattcgcaa aggccagggt gtcactccta
    15481 ccgcatttgc gatgcatcta catgagtata tcagtcaggg ccttgagtcc attcttggcg
    15541 cgctggatat cgaaggaagc tatgataagc aacgaacgat aactattgcg accactccct
    15601 cggtcggagc cttggtcctt cctgtcatct atcgggcgat taaaactcac tatccgcagc
    15661 ttttactgcg caacccgccc atcagcgacg cggaaaacca actcagtcag tttcaaaccg
    15721 atctcatcat cgataacatg ttttgcacca atcgtacggt gcaacatcat gttctgttca
    15781 ccgacaatat ggtgttaatt tgccgcaagg gaaatccact actctcttta gaagatgaca
    15841 gagagactat cgacaacgct gcgcatgtac ttctgttacc ggaagggcaa aatttcagcg
    15901 gtctgcggca gagagttcaa gagatgtttc cggaccggca aatcaatttc accagctaca
    15961 acattttgac aatcgctgca ctggttgcca acagtgacat gttagcgatt attcccagcc
    16021 gtttttataa cctgtttagt cgttgctggc cgctggaaaa actgcctttt ccgtccttaa
    16081 atgaggagca aatagatttc tctatccact acaacaaatt catcctgcgt gatcccatcc
    16141 tgcacggagt aatagatgtt atccgtaatg cattttgaaa aagtattgtt acagccagat
    16201 cacagcgcag aacgataaaa tggcacaatc caacaacaat tttacatttt cgcgaccgct
    16261 ttggctgctt tcaggtccgt ttcaatgata tactgccagt cgttaattca aaaatagttg
    16321 ataattacaa caatctattg aattgaaacg ctttccttcg taattcgcaa ctggaacacg
    16381 cacgctatga gtaaacccat tgtgatggaa cgcggtgtta aataccgcga tgccgataag
    16441 atggccctta tcccggttaa aaacgtggca acagagcgcg aagccctgct gcgcaagccg
    16501 gaatggatga aaatcaagct tccagcggac tctacacgta tccagggcat caaagccgca
    16561 atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct cctgccctaa cctggcggaa
    16621 tgcttcaacc acggcacagc aacgtttatg atcctcggcg ctatttgtac ccgccgttgt
    16681 ccgttctgtg atgttgccca cggtcgcccg gtagctcctg atgccaatga accagtgaaa
    16741 ctggcgcaga ccattgccga tatggcgctg cgttatgtgg ttatcacctc cgttgaccgt
    16801 gatgacctgc gcgatggcgg tgcccaacac tttgcggatt gtattactgc cattcgggaa
    16861 aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt tccgcggtcg aatggatcgt
    16921 gctctggata ttctgactgc aacgccccca gatgtgttca accataacct ggaaaacgta
    16981 ccgcgtattt accggcaggt acggcctggc gcagattaca actggtcgct gaagctgctg
    17041 gaacgcttta aagaagcgca tccggaaatc ccgaccaagt ctggtctgat ggtgggtctg
    17101 ggtgaaacca atgaagaaat tattgaggta atgcgcgacc tgcgccgtca tggtgtgacg
    17161 atgttaacgc tggggcaata tttgcagcca agccgccatc acctgccggt tcaacgttac
    17221 gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc tggcgatggg ctttacccat
    17281 gctgcatgcg gtccgtttgt ccgctcttct taccacgccg atttgcaggc gaaaggaatg
    17341 gaagttaagt aatcctgtaa taattactta catctgtctg ataaaaaacc cgctttttga
    17401 agcgggtttt ttgtatcgaa caagatattg agggagcgtc ctgctcgcca cgtcactctt
    17461 tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc ttttttcgcc gcagcatcgt
    17521 catcattcat cgccttcttg aaccctttaa tggccgctcc aaggtctccg cccagcgtac
    17581 gtaacttctt agtcccaaac agcagaacga ccagcgccgc aactaccagc agtttggtaa
    17641 tactaatctc acccatagat accttcttga catataaccg gcaacagaac gcgccttaaa
    17701 aaccagagaa tattaacggt cattcgacgc gcgcacacaa tagcaatctg taacaaggtg
    17761 aatcaagcgc cgagtaaaaa aacatcataa taattgcggc ggcgcaaagc gacggttgtt
    17821 taagatgggc agttgcgcgc gcacctgacg cacacgttcg ggcgtcactt ccgccataat
    17881 gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca aagggatcta taatccggct
    17941 ttgcccgata tttttgttcc cgcactcccc cgccgccacc atataacagg tggtatccag
    18001 cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg agcggcccac gaacccaggc
    18061 ggcaggaagt accaggattt cagctccctg taaagcctgt gccagcgcca gctctggaaa
    18121 gcgtaagtca taacaggtca tcagaccgac cttcatccct tccacctcca gtaacggagc
    18181 tatttcatta ccagcatcaa catggcgtga ttcctgaatg gcaaatgcat catagagatg
    18241 cagtttggca taacgggcga cgatgtttcc tgcctgaagt gccaccagca tattccatgc
    18301 gcgccccggc gttgaaggaa gatgaatcgt cagaattgtc gtcatcatgt tacgtttact
    18361 ttctcgccgt aaacgtccga ggaattcgcc ttccagcagc tgtgctgatt taaccgatag
    18421 atctgcatca tgatcatcgc gcgccagcaa tgcttccggc aggacaaaca gcgatacgtc
    18481 gttttccgcc gcctgcgcca tcaacgaggc acaaatctca gcgttctttt cccacacaga
    18541 tgtaacagca aactgtcctg cagcaaccag cattgccacc tcctgtcaga gtcgtaaaat
    18601 tcgccaacga cgattattcg gcgttacact tatcactcat acaaatcaaa tagcaggatt
    18661 ttgcagtgtt acaacttctt ttagcagttt ttattggcgg tggtacggga agcgtagcga
    18721 gatggctgtt aagtatgcga tttaacccgc tgcatcaggc gattccgttg gggacgctgg
    18781 cagcaaacct gattggggca ttcatcatag gaatgggatt cgcctggttc agcaggatga
    18841 cgaacattga tccagtgtgg aaagtattaa tcaccaccgg attttgtggc ggtctaacaa
    18901 ccttctcaac attttcggca gaagtggtgt ttttgttaca agagggccgc tttggctggg
    18961 cattactgaa cgttttcgtc aaccttctgg ggtcttttgc catgaccgca ctggcattct
    19021 ggctgttttc ggcctcaacc gtacactaaa gaccaaaaaa aacccgctga ttaagcgggt
    19081 tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa gggcctttgg
    19141 caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta aaaccattag
    19201 tctggattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga gtaatgaaac
    19261 cgaatccttt ggactcatta aaccacttaa cgttaccttt aatcttagac atcaaaatta
    19321 cctttacatg aaaaatagac acaaatgctg tgtcggttat cagtacacca attgtggtac
    19381 gctttgtcca gtgcaacttt actaaaaagt gataaaagtc gcgttatttt tgatgttgct
    19441 tattttagtt attgatttta aagaagttac taaaacttca tttgtctcaa aactgaaagc
    19501 gcatccaggc aaagtacaca ttgccattgt tgtaggtacc cggaatgtag gtcatctgaa
    19561 aagtcactgg gccataaccc acggaggcca atggcagtag aaccgggaga gggatgtaat
    19621 tccagttatc gcgtgccgtt acgccagcgg tgaatcccag acctaaatga aaattttcat
    19681 ccgccagcgg tcgccaggta ctttcccatc cgtatccggc aatcggttcc catttgttcc
    19741 acgagtcctt aaatgccatg gcatacaggc catgccagtt tcctttttca tcccaacgcg
    19801 acaggccaaa accgccaccc cacggtcgct cgttatagcg atcggttttt tctttgtcgt
    19861 aagcgaaacg tgcatgccag gtgatggcag gaatatataa atcataatgt tcaggctgtt
    19921 gccaggtttg tgcaatattt tctctaaacg ttgtcatcct ctcatctgcg ttagcaaaaa
    19981 ctttaccaac gctgattaac tgaataaaaa caaaggaaaa gatagcgaca tatttactca
    20041 cgttcatttg tgaccataaa acatttatca aaaatctact actagcatag caaagctaca
    20101 attaacataa ccttaataga cccaacataa agaataatct gaatagctgc tctatttatt
    20161 tccgcttgtt caagttaaat atttccgcgc atcgattaaa gatgatgcgt cgttttctgc
    20221 gatgggaata gtcaaaaaag aaaaaccaag tcttttttga tgacaaaatg caatcaagga
    20281 aaaattattt tattttttta acgagataca acaatcatct taacgaagta tataatatat
    20341 atcgtcaacc catgctcata ttagattatt ttttgagcgc acagccgcag ctaacaccat
    20401 tgcaattaac aaatttgcat caatccacca tcaatttaca cacattatta tgtgataatt
    20461 gccaaccgct aaatatgcgt tttgttatct atgtataaaa acagcaactt caatgtctta
    20521 atggcagttt ttcttgattt taatcagcat tcatcgccaa tttattgggc atattttttc
    20581 cttaagcttt aggaagtttt tattatttac tttgggcctg gagacaggaa aaattatgct
    20641 gacattcatt gaactcctta ttggggttgt ggttattgtg ggtgtggctc gctacatcat
    20701 taaagggtat tccgccactg gtgtgttatt tgtcggtggc ctgttattgc tgattatcag
    20761 tgccattatg gggcacaaag tgttaccgtc cagccaggct tcaacaggct acagcgccac
    20821 ggatatcgtt gaatacgtta aaatattact aatgagccgc ggcggcgacc tcggcatgat
    20881 gattatgatg ctgtgtggct ttgccgctta catgacccat atcggcgcga atgatatggt
    20941 ggtcaagctg gcgtcaaaac cattgcagta tattaactcc ccttacctgc tgatgattgc
    21001 cgcctatttt gttgcttgtc tgatgtcact ggccgtctct tccgcaaccg gtctgggggt
    21061 tttgctgatg gcaaccctgt ttccggtgat ggtaaacgtt ggtatcagtc gtggcgcagc
    21121 agctgccatt tgtgcctccc cggcggcgat tattctcgca ccgacttcag gggatgtggt
    21181 gctggcggcg caggcttccg aaatgtcgct gattgacttc gccttcaaaa cgacgctgcc
    21241 tatctcaatt gctgcaatta tcggcatggc gatcgcccac ttcttctggc aacgttatct
    21301 ggataaaaaa gagcacatct ctcatgaaat gttagatgtc agtgaaatca ccaccactgc
    21361 ccccgcgttt tatgccattt tgccgttcac gccgatcatc ggtgtactga tttttgacgg
    21421 taaatggggt ccgcaattac acatcatcac tattctggtg atttgtatgc tgattgcctc
    21481 cattctggag ttcctccgca gctttaatac ccagaaagtt ttctctggtc tggaagtggc
    21541 ttatcgcggg atggcagatg cgtttgctaa cgtggtgatg ctgctggttg ccgctggggt
    21601 attcgctcag gggcttagca ccatcggctt tattcaaagt ctgatttcta tcgctacctc
    21661 gtttggttcg gcgagtatca tcctgatgct ggtattggtg attctgacca tgctggcggc
    21721 agtcacgacc ggttcaggca atgcgccgtt ttatgcgttt gttgagatga tcccgaaact
    21781 ggcgcactct tccggcatta acccggcgta tttgactatt ccgatgctgc aggcgtcaaa
    21841 cctgggtcgt accctatcac ccgtttctgg cgtagtcgtt gcggttgccg ggatggcgaa
    21901 aatctcacca tttgaagtcg taaaacgcac ctcggtgccg gtgcttgttg ggctagtgat
    21961 tgttatcgtt gctacagagc tgatggtgcc aggaacggca gcagcggtca caggcaagta
    22021 aatagtaatg ccggaggcaa gtttcctccg gctttatcat gtcacccact gtggtatatg
    22081 cgttgtggtc tgccaacttt gccgtgaaca atttcagcaa taatcagatg gcggctggcg
    22141 caatattcaa gataacgcct ggcagtggtg cggctgatgg tcagtgcctg cgccaccgtt
    22201 tctgccgtat gttgcacacc aggctcttta aacagttttc gcaccgcgtt tagcgtcaag
    22261 gggtcaatgc cggtcggcag ttcgtctttg ggttcaccgc gagcataggc attaaacatc
    22321 tcatcaattt gcttctggct ggcgctatca atactttcca gcatatgttt acgctggcgg
    22381 aaacgggtta gcgtttgccc cagtgtattg attcacttga agtacgaaaa aaccgggaga
    22441 acattggatt attcgggatc tgatgggatt agatttggtg gggcttgcaa gcctgtagta
    22501 caaattttag gcgttaacca atgaaacgcg aaagatagta aaaaattgct tttgtttcat
    22561 tgaaaaaacg aaaaacaaaa acactataaa tcatttcaat catagcttca aaaaacgttc
    22621 aaaaccgata acaaccaggc tgtcatcaaa tgactcatat cacaaatcag ctcatgccgt
    22681 ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg ctcatggttg
    22741 ttatacggct tttttcgcat cttgtggttc caacaaagga gctacaggct cttcctgttt
    22801 atgaacacca tacattgaca aaatctcttg gcgagtttct ggaggtaatt tatgcagcgc
    22861 caaagctgtt tgtagtgttt ggcgatcaat acccagcgtt gccaataggg tgtcaacgga
    22921 gatggagggg cgttcttgca ttaatccgga aagcccatat ttagccatgt ttagctctat
    22981 ctctcggact tcattttctc ctagtttatt gattaacagg gagaacattt tcaatgtatc
    23041 agcattcatt tcttcgatag caattttttg catagccggt attgccggga tatattcaac
    23101 cccaggcccg cgacgccctt cttgtgaacg cttcggccat ccttctttct ttgctcgttt
    23161 atgcaccccc tgtattgtcc caggcattcc atccagttca gtaatttgtt tgggtgtcat
    23221 ccactggttt atctcatttg cgcttttcat aaactaagtt taccagattt atctatataa
    23281 aaatcaatgg attacaaaat aaaccacatt aactgatata aaccggtttg gtttatttgg
    23341 taatgtataa aacagataat caagacgaat tatttgtttt ggtaaccctt tatcatacgc
    23401 aatatggttg gtgtgcgaaa gatgagtaac gattggcatc gggccgacgt gattgcggcc
    23461 ctcaaaaaaa caggaacccc tctggcaaaa ttgtccagac gttcaggtta ttcaaccggg
    23521 acgcttgcaa atgcattagt caggccatgg cctaaaggtg aacgcattat tgctgacgcc
    23581 ttaaacatga aacctgagga aatttggcct agtagatatc taagggatca caatgagaga
    23641 gtggatatca gcaattgaat gcatgggttt atctggttta cctggttcat taatgggggt
    23701 tcacaaaaga gctcagagag aaggatggct caagagaaga agagaaggaa ttagaggggt
    23761 tgcttacgaa taccatatat cttctcttcc taacattgct cgagctgaag ttttgctcca
    23821 acaaggaaaa tttaaaacca gtcagggatg ttttgaaatc gcccgcccca cgctggaagc
    23881 tcatgattat gatcgtgagg cactatggag caaatgggat aaagccagcg attcccagcg
    23941 cagcctggct gaaaaatggt tgccctcaat tcaggccaca gacgaaatgc tgaaccaggg
    24001 gatttcaacg aaaacggctt ttgcgaccgt tgcagggcat taccaggtca gcgcatccac
    24061 tttgcgggac aagtattacc aggtacagaa gtttgcgaag cctgactggg cggctgcact
    24121 tgttgatgga cgtggagcat cccgtcgcaa tgttcacaaa agtgaatttg acgaggatgc
    24181 ctggcagttt ctgattgcag attatctgcg accggaaaaa cccgccttcc gcaaatgtta
    24241 tgagcgtctg gaactggcag cccgcgagca tggctggagt attccctccc gtgccacggc
    24301 ctttcgccgg attcagcaac tggacgaggc aatggttgtt gcctgtcgtg aaggtgaaca
    24361 tgcactgatg catctgatac cggcacagca gcgaactgtg gaacacctgg acgccatgca
    24421 gtggatcaac ggcgacggtt atctgcataa cgtctttgta cgctggttta acggtgatgt
    24481 gatccgtccg aaaacatggt tctggcagga tgtgaaaacc cgaaaaattc tgggctggcg
    24541 ctgcgatgtg agcgagaaca ttgattcaat tcgcctctcg ttcatggatg ttgtgactcg
    24601 ctacagtatc ccggaggatt ttcacatcac cattgataac acccgtggtg ctgcgaataa
    24661 atggctgacg ggaggcgcgc ccaatcgcta ccgctttaag gtaaaagagg acgatccaaa
    24721 aggactgttt ttactgatgg gtgcgaaaat gcactggaca agcgttgttg ccggtaaagg
    24781 ctggggccag gcaaaacctg ttgaacgtgc tttcggtgtt ggtgggcttg aggaatacgt
    24841 tgataagcat ccggcactgg ctggcgcata tacggggcca aatccgcagg caaaacctga
    24901 taactatggc gaccacgctg ttgatgcaga gctgtttctg aaaacctttg ccgaaggtgt
    24961 ggcgatgttc aatgccagaa caggccgtga aacagaaatg tgcgggggca aactctcgtt
    25021 tgatgatgtt ttcgagcgtg aatacgccag aacgattgtg cgtaagccaa ccgaagaaca
    25081 aaaacggatg ctgttactgc ctgccgaggc ggtgaacgtt tcacgcaaag gcgagtttac
    25141 gcttaaagtt ggcggctccc ttaaaggcgc gaaaaacgtt tattacaaca tggcattaat
    25201 gaatgccgga gtgaaaaaag ttgtggtcag atttgatcca cagcagttac acagcacg
//
LOCUS       DAJTUP010000055        25199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTUP010000055 DAJTUP010000000
VERSION     DAJTUP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.055"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1056)
                     /gene="yeaG"
                     /locus_tag="OO468_002299"
     CDS             complement(<1..1056)
                     /gene="yeaG"
                     /locus_tag="OO468_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR6259390.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELT"
     gene            1492..2238
                     /gene="mipA"
                     /locus_tag="OO468_002300"
     CDS             1492..2238
                     /gene="mipA"
                     /locus_tag="OO468_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR6259391.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2328..3089
                     /locus_tag="OO468_002301"
     CDS             2328..3089
                     /locus_tag="OO468_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR6259392.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3140..4024)
                     /locus_tag="OO468_002302"
     CDS             complement(3140..4024)
                     /locus_tag="OO468_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR6259393.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4108..5103)
                     /gene="gapA"
                     /locus_tag="OO468_002303"
     CDS             complement(4108..5103)
                     /gene="gapA"
                     /locus_tag="OO468_002303"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR6259394.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5445..5858
                     /gene="msrB"
                     /locus_tag="OO468_002304"
     CDS             5445..5858
                     /gene="msrB"
                     /locus_tag="OO468_002304"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR6259395.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            5900..6172
                     /locus_tag="OO468_002305"
     CDS             5900..6172
                     /locus_tag="OO468_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR6259396.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6542..7618
                     /locus_tag="OO468_002306"
     CDS             6542..7618
                     /locus_tag="OO468_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR6259397.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7645..9024
                     /locus_tag="OO468_002307"
     CDS             7645..9024
                     /locus_tag="OO468_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259398.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9041..10086
                     /locus_tag="OO468_002308"
                     /pseudo
     CDS             9041..10086
                     /locus_tag="OO468_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10107..10943
                     /locus_tag="OO468_002309"
     CDS             10107..10943
                     /locus_tag="OO468_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR6259399.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            10948..11895
                     /locus_tag="OO468_002310"
     CDS             10948..11895
                     /locus_tag="OO468_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR6259400.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            11905..12885
                     /gene="ydjG"
                     /locus_tag="OO468_002311"
     CDS             11905..12885
                     /gene="ydjG"
                     /locus_tag="OO468_002311"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR6259401.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13022..13780
                     /locus_tag="OO468_002312"
     CDS             13022..13780
                     /locus_tag="OO468_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6259402.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            13898..15256
                     /locus_tag="OO468_002313"
     CDS             13898..15256
                     /locus_tag="OO468_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259403.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15349..15990)
                     /gene="pncA"
                     /locus_tag="OO468_002314"
     CDS             complement(15349..15990)
                     /gene="pncA"
                     /locus_tag="OO468_002314"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR6259404.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16001..17017)
                     /gene="ansA"
                     /locus_tag="OO468_002315"
     CDS             complement(16001..17017)
                     /gene="ansA"
                     /locus_tag="OO468_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR6259405.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17184..19040)
                     /gene="sppA"
                     /locus_tag="OO468_002316"
     CDS             complement(17184..19040)
                     /gene="sppA"
                     /locus_tag="OO468_002316"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR6259406.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19201..19752
                     /locus_tag="OO468_002317"
     CDS             19201..19752
                     /locus_tag="OO468_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR6259407.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            19869..20912
                     /gene="selD"
                     /locus_tag="OO468_002318"
     CDS             19869..20912
                     /gene="selD"
                     /locus_tag="OO468_002318"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR6259408.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            20917..22878
                     /gene="topB"
                     /locus_tag="OO468_002319"
     CDS             20917..22878
                     /gene="topB"
                     /locus_tag="OO468_002319"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR6259409.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23005..24045
                     /locus_tag="OO468_002320"
     CDS             23005..24045
                     /locus_tag="OO468_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6259410.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24162..>25199)
                     /gene="gdhA"
                     /locus_tag="OO468_002321"
     CDS             complement(24162..>25199)
                     /gene="gdhA"
                     /locus_tag="OO468_002321"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR6259411.1"
                     /translation="LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRF
                     CQTLMTELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGS
                     LIRPEATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITAS
                     DSSGTVVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALP
                     CATQNELDVDAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVA
                     TSGLEMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVK
                     VADAMLAQGVI"
BASE COUNT         6407 a         5849 c         6501 g         6442 t
ORIGIN      
        1 agtcaattca ctgtgattaa gcaatttctc gtagattttg atctcttcgg aaatgcgcaa
       61 gcaatacggc accttcacga tgtaaacacg gtcgaggaag gcttcgttgt ttttgttatt
      121 acggaaagtg acccattcgg acttgttcga gtgggcgaga ataatcccgt tgaacggcag
      181 ggcggagata ccttccgtcc cgttgtagtt accttcctga gtggcggtta acaagggatg
      241 cagcactttt atcggtgctt taaacatctc aacgaattcc atgatcccct gattggcgcg
      301 gcacagcgca ccggaatagc cgtaggcgtc cgggtcattc tgcgcgtagt gttcgagttt
      361 acgaatatcg actttcccaa ccagcgcgga gatgtcctgg ttgttctcat caccgggttc
      421 cgttttggcg atagcaattt gttgcagaat tgacggccag accttcacta cccggaactt
      481 agtgatatcg ccaccaaatt catgcaggcg ttttgccgcc catggcgaca tgatggtgcc
      541 gagataacgg cgaggaatgc catactcttt ttccagaatc tgagcatctt cctgcggatt
      601 gaaaagacag aacggatgat cgttgaccgg gctacgctca ccgttggcgc tcaatacata
      661 aatcggtacg agctgcatta atgatttcag tcgctcagca agcgatgatt tacccccacc
      721 cacaggcccc agcagataca ggatttgttt cttctcttcc agcccctgag ccgcgtgttt
      781 cagataagag acaatctgtt caatcgcgtc ttccatgccg taaaactctt caaatgccgg
      841 ataacgtgca atgacccggt tagaaaagag tcgagaaagt ctggggtcct gggctgtatc
      901 gaccatgaca ggctcaccga tagccatcaa tagccgctca gccgcgttgg cataagcact
      961 gcgatcttgc cgacaagtgg taagaaactc ctgcagtgtg aactcttcgt ccttggcagc
     1021 ttcatatcgc tggcgatagt gatcgaatat attcatggta tgccgtcctt tcgtttttta
     1081 gcacaggtta agagccgttc atatgaatag tagaggctcc cggaagaggt aaactgaacg
     1141 acgtctcttg ctaaaacaca gcaactctca tgccatttta tcgacgatga gaacaacgtg
     1201 cccggtgata caccttcaaa attaagcgta gatggcaaat ggaaaacttg cctgcacggc
     1261 tttcttaatt tcaatggcat atcaacaact catccataaa ttttgcataa ttaatgtaaa
     1321 gaccaggctc gccagtaacg ctaaatccat ttggctgtaa gcgcggtgtc atccgcggca
     1381 ggaaaattaa acagttactt taaaaaatga aaacgtaaaa aggttgggtt tcgatgtatt
     1441 gacgggtaaa ctttgtcgcc cgctaaacat ttgtttaagg aatgattaat tgtgaccaaa
     1501 ctcaaacttc tggcacttgg agtgcttatc gcaacgtctg caggcgtagc gcacgctgaa
     1561 ggtaaatttt ccctgggcgc aggcgtaggt gtcgttgagc acccatataa agattacgat
     1621 accgatgttt acccagtacc ggtaatcaac tatgaaggcg ataacttctg gttccgtggc
     1681 ttaggtggtg gttactacct gtggaatgac gcaacggata aactttcaat taccgcttac
     1741 tggtcgccgc tttacttcaa agcgaaagac agtggcgatc accaaatgcg tcacctggat
     1801 gaccgtaaga gcaccatgat ggctggtctg tcttatgctc actttaccca gtacggttac
     1861 ctgcgtacca ccctggctgg cgataccctg gataacagca acggcattgt ctgggatatg
     1921 gcctggttgt atcgttacac caacggtggc ctgaccgtga ctccgggtat tggtgtgcag
     1981 tggaacagcg aaaaccagaa cgaatactat tatggcgtat cgcgcaaaga gtccgctcgc
     2041 agcggtctgc gtggctataa cccgaatgac agctggagcc cttaccttga gctgagcgcc
     2101 agctacaact tcctcggcga ctggagtgtt tacggtaccg cgcgctacac ccgtctgtct
     2161 gatgaagtta ctgacagccc gatggtggat aaatcctgga ctggcctgat ttctaccggg
     2221 atcacctaca aattctgata atgcatcttt ccagggcatt ttgtgcataa aaacagggcg
     2281 tcacgccctg ttttgcatta cggtggtgca atacctggga ggaaacaatg caacaaaaaa
     2341 tgattcaatt tagtggcgat gtctcactgc cagccatagg gcagggaaca tggtatatgg
     2401 gcgaagatgc cagtcagcgc aaaacagaag ttgctgcact acgcgcaggc attgaactcg
     2461 gtttaaccct cattgatacc gccgaaatgt atgccgatgg cggtgccgaa aaggtggttg
     2521 gggaagcatt aaccggtctg cgagagaagg tctttctcgt ctctaaagtc tatccgtgga
     2581 atgctggcgg gcaaaaagcg ataaatgcat gcgaagccag tttacgccgt ctcaatactg
     2641 actatctcga tctctactta ttacactggt ctggcagttt cgcttttgaa gagactgttg
     2701 ctgcgatgga aaaattgatc gcccagggaa aaatccgtcg ctggggcgtt tctaaccttg
     2761 gttatgccga tatgcaggaa ctctggcagc tgccgggggg aaatcagtgt gccactaatc
     2821 aggtgcttta ccatctcggt tcacgaggga ttgagtacga tctactcccc tggtgccagc
     2881 aacagcagat gccggtgatg gcttacagtc cattagccca ggccgggcgg ttgcgcaatg
     2941 gactgttaaa aaacgcggta gtcaacgaaa ttgcacatgt ccaacaacat gcagctgcgc
     3001 ttgaggtcga actttcgtca gcggaattag ctatgctgga taaggcatat ccggcaccaa
     3061 aaggaaaaac tgcgctggat atggtgtgat gttgatacgt gccggatgca tcgcacccgg
     3121 cacacaacat taacgcaaat taacgtttgg caacgcgaat ggattgcgcc agatgtgcag
     3181 gtttctcttt ggtgactttt tgcgtttctg aagcgtaagc cgtttctaca caaacaaagg
     3241 tcttgtagcc atcgtccggc atgtcgccca tgctaactga aagcgccggt cccgggttcc
     3301 agccgacaac gttcagatga tgctggtggc ctacggcgat aatacgattc agcgcttcat
     3361 cattaatcac gctgcaatct tgcggattca gatacacgcg atcggtacgg tcagggaagg
     3421 tctgaatacc gtcggtcagt acatcttctt tcgcgtcatt cactttatca atgaagcgat
     3481 cgcccagccc actgacgctt accttagcga tatcacccac gttaaagtag gtatgcaggg
     3541 cagaggtggt ttcaaattcg ccatgtgatt caagatcgat ttcacaggtt ttacccacgc
     3601 ggaaatgcgc taacagcgta aagtcgtgcg gccagaattt tttcgtctct tcgctttgtg
     3661 tcaattcaaa agtcagcgct acgccatcag catcttcatg atgtgatttc agcgtccacg
     3721 gcaggttgcg ggcaaaaccg tgcgcaggca gaccttgttg tgccgccgga ccaaaccacg
     3781 gccagcaaac cggtacgcca ccgcgaatag cgacgccatt tttgaacggt gtgttgttgc
     3841 tcaaccacag aacttcttct tcacccgcag gtttccacga gagaaggtgt gcgccctgta
     3901 atgcaaaaga ggcttttacc tggggatgat cgaccacaat gaggtccagt tcatccagtt
     3961 tacgacggga gaggacaggg gagatttgtt cgatgaccgg aagggcaaaa attttcttaa
     4021 tcatgacgca gtcctttaac ttcattttat caggtaaaaa aaagagcgac cgaagtcgct
     4081 ctttttagat cacagtgtca tctcaactta tttggagatg tgagcgatca ggtccagaac
     4141 tttgttggag taaccggttt cgttgtcgta ccaggatacc agtttcacga agttgtcgtt
     4201 cagagcgata ccagctttag catcgaacac ggaagtgcaa acttcgccgt tgaaatcggt
     4261 agatactacg tcatcttcgg tgtagcccag aacgcctttc atttcgcctt cagcagcagc
     4321 tttaacggca gctttgatct gctcgtaagt tgcagctttt tccagacgaa cggtcaggtc
     4381 aactacggat acgttcgggg tcggaacgcg gaacgccata ccagtcagtt tgccattcag
     4441 ttctggcagt actttaccta cagctttagc agcaccggta gaggacggga tgatgttctg
     4501 ggaagcgccg cggccgccgc gccagtcttt gtgagacggg ccatcaacgg ttttctgagt
     4561 agcggtagta gcgtgaacgg tggtcatcag accttcgatg atgccgaagt tatcgttgat
     4621 aactttagcc agcggagcca ggcagttggt ggtgcaggaa gcgttggaaa cgatgtcctg
     4681 gccagcatat ttgtcgaagt tagcgccttt aacgaacatc ggagtgttgt ctttagacgg
     4741 accagtcata accactttct tcgcaccagc ggtgatgtgt ttacgagcag tttcgtcagt
     4801 caggaacaga ccagttgctt cagcgacaac gtcaacaccc acttcgtccc atttcaggtt
     4861 agccggatca cgttcagcgg taacacggat ttttttaccg ttaacgatca gatgaccgtc
     4921 tttcacttca acggtaccgt cgaaacggcc gtgagtggag tcatatttca gcatgtatgc
     4981 catgtaatca gcgtctaaca ggtcgttgat tgcaacgatc tcgatgtcag aacgtttctg
     5041 agcagcacgg aaaacaatgc gaccgatacg gccaaaaccg ttgataccta ctttgatagt
     5101 catatattcc accagctatt tgttagtgaa taaaaggttg cctgtaaaat tacaaaaacc
     5161 ttacgcagcg tcaagcggaa tcgtgtcaat cattgcgaca aatcaatcct gtgcctaagc
     5221 attacgcgac tgactcgcct cacttttcct ttgggcttga gaccacatgg ggtcggcgcc
     5281 ccgaatttta aagggcaatt acgataaaaa tgtgattttc atcacgattt aggtgcagcc
     5341 atttcgtcga gatcaagttt aggacaaaat tttgccagtt ggtgttaatg ttttgttaga
     5401 atcagtcacg taatgtgagc acgattaaag tgagatgtga gtaaatggcg aataaacctt
     5461 cggcagaaga actgaaaaaa aatttgtccg agatgcagtt ttacgtgacg cagaatcatg
     5521 ggacagaacc gccatttacg ggtcgtttac tgcataacaa gcgtgacggc gtatatcact
     5581 gtttgatctg cgatgccccg ctgtttcatt ccgaaaccaa gtatgattcc ggctgtggct
     5641 ggcccagttt ctacgaaccg gtgagtgaag aatccattcg ttatatcaaa gacttgtcac
     5701 atggaatgca gcgcatagaa attcgttgcg gtaactgtga tgcccatctg gggcatgtct
     5761 ttcccgacgg gccgcaacca acaggcgaac gttattgtgt taactctgct tctttacgct
     5821 tcactgatgg cgaaaacggc gaagaaatca acggttgaaa aaacgattca gcaacttatt
     5881 ccacaggagc ggaaaaatta tgaatcttga tgacattatc aacagcatga cgcctgaggt
     5941 ataccagcgt ttgtcgaccg ccgttgaact ggggaaatgg cctgatggcg ttgcgttgac
     6001 cgaggaacaa aaagagaatt gcctgcaact ggtgatgctg tggcaagccc gccataatac
     6061 cgaagcacag catatgacaa ttgacacaaa tggtcagatg gtcatgaaga gcaaacagca
     6121 gttaaaagaa gattttggta tctcagcaaa gcctattgcc atgtttaagt aagtggttca
     6181 tccccttttt gccgcggcta tgaaggggat agccaacaat gaccccacga ctcgtctcaa
     6241 ttatctcgac ccgacctggc taagtcccag tccgatctct tgcagtcgcg ttcacgcctg
     6301 aatgacttct cctgaactta agtctcagac ctatttggcc ggtaatccct ctcgaatttg
     6361 ctctttttgc gtcccgcctt ccaccaacct gtaacagacc cagccatttt ctgcgtaatt
     6421 gtgatttgag ttgtaaaatg caggtcatta tctgtgttgt gcagatttaa tattgacctc
     6481 aaaaatcatg tgggtaagta tgatggccat ctaagcaaaa caaaacacag aggatgacgc
     6541 aatgaaagca ctggctcggt ttggcaaggc ctttggcggc tacaagatga ttcatgtccc
     6601 acaacccatt tgtggcccgg aagacgtagt gactgaaatt aaagccgcgg caatctgcgg
     6661 cgcagacatg aagcactaca atgtcgatag cggttctgat gagtttaact ctatccgcgg
     6721 ccatgagttc gcaggttgta ttgcgcaggt tggtgaaaaa gtcaaagact ggaaagtggg
     6781 gcaacgcgtc gtgtcggata acagcggtca cgtttgcggc gtttgtccgg cctgtgaaca
     6841 gggtgatttt ctgtgttgta cagaaaaagt aaaccttggt ctggataaca atacctgggg
     6901 cggtggtttt tccaaatatt gtctggttcc tggtgaaatt ctcaaaattc atcgtcatgc
     6961 gttgtgggaa atccctgatg gtgttgatta tgaggacgca gccgtacttg accctatctg
     7021 taatgcctat aaatccatcg cgcagcaatc gaaattcctt cctggtcagg atgttgtcgt
     7081 cattggcact ggcccactcg ggctgttttc cgtacaaatg gcgcggatta tgggggcggt
     7141 aaatatcgtc gtcgttggtc tgcaagaaga tgtggcggtc cgcttcccgg ttgcaaaaga
     7201 actgggggcg acggcagtag taaatggttc taccgaagat gtggtggcgc gctgccagca
     7261 aatttgtggc aaagataatc tgggactggt gattgaatgc tccggtgcca atatcgcact
     7321 gaaacaagcc atcgaaatgc tccgcccgaa tggggaagtg gtacgcgttg gaatgggctt
     7381 caaacctctt gatttctcga ttaatgacat taccgcctgg aataaaagca tcattgggca
     7441 tatggcctat gactccacct catggcgtaa cgctatccgg ctattagcca gcggcgctat
     7501 caaagtcaaa ccgatgatca cgcatcgtat cggcctgtcg caatggcgcg aagggtttga
     7561 tgcgatggtc gataaaaccg caatcaaagt gatcatgact tacgactttg atgaataact
     7621 actctcctga aggaacgtta tcgcatggaa cagataacaa aaccgcattg tggtgcacgg
     7681 ctggatcgct taccggattg ccgctggcat tcgtcaatgt ttgccatcgt cgcgtttggc
     7741 ttgcttgtct gctggagtaa tgccgttggt ggcttgatcc tcgcgcagct gaaagcgttg
     7801 ggctggacag ataattccac tacagccaca ttctcagcaa tcacgaccgc cggaatgttt
     7861 ctcggtgcgc tggtgggcgg catcattggc gacaaaaccg gtcgcagaaa tgcgttcatc
     7921 ctctatgagg ccattcatat tgcctcgatg gttgtcggtg ctttctcacc gaatatggat
     7981 tttcttattg cctgccgttt tgtgatggac gttgggctgg gagctttact ggttacactg
     8041 tttgctggtt tcaccgaata tatgcccggt agaaatcgcg gaacgtggtc cagtcgggtt
     8101 tcctttattg gcaactggtc atatccgctc tgctcactga ttgcgatggg actcacgccg
     8161 ctgattagtg cagagtggaa ctggcgggta caactgctta ttcctgcaat attgtcgctt
     8221 atcgctacgg cgctggcctg gcgctacttt cctgaatccc cgcgctggct ggaatcgcgc
     8281 ggacggtatc aggaagccga gaaagtcatg cggagtatag aagaaggcgt catacgccag
     8341 acgggtaaac ctttgccgcc tgtggttatt gctgatgacg gtaaagcgcc acaagcggtg
     8401 ccgtattcag ccttactgac aggagtatta ctgaaacgcg tgatattagg ctcttgtgtg
     8461 ctgattacca tgaacgttgt gcagtacaca ctaattaact ggttgccaac aatattcatg
     8521 acccagggga ttaatttaaa agactcgatt gttttaaata ccatgagtat gtttggcgcg
     8581 ccatttggta tttttattgc catgctggtg atggataaaa ttccgcgtaa aacaatgggt
     8641 gtggggctat taatcctgat tgcggtgctc ggatatatct attcactgca aaccagtatg
     8701 ttgctcataa cgctgattgg tttcttcctg attactttcg tctatatgta cgtttgctat
     8761 gcctcggcag tgtatgtccc tgaaatctgg ccgacagagg ccaaactccg tggctccggt
     8821 ctggcgaatg cggtagggcg aatcagtggt attgccgcac cttatgccgt tgcagtgctg
     8881 ctcagtagtt atggtgtaac gggagtcttt attcttctgg gggctgtttc aaatattgtc
     8941 gcaattgcta tcgccaccat tggaattgaa accaaaggtg tctccgttga aagtttaagt
     9001 attgatgcag tagccaataa ataatacggg tggattcaaa atgaaaaatt caaaagcaat
     9061 attgcaggtg ccgggcacaa tgaaaattat ttcagcagaa ataccagtgc ctaaagaaga
     9121 tgaagttttg attaaagtag aatatgtcgg tatttgtggt tcagatgtac atggttttta
     9181 atcaggcccg tttattccgc ctaaagaccc aaatcaagaa attggcctgg gtcatgaatg
     9241 cgccgggacg gttgtggctg tgggaagccg cgtgcgcaaa tttaaaccgg gggatcgggt
     9301 aaatatcgaa cctggcgttc cttgcggtca ctgtcgttac tgtctggaag gcaaatataa
     9361 catctgcccg gacgttgatt ttatggcgac acaacccaac taccgcggcg cattaacgca
     9421 ctatctgtgt catccggaga gctttactta caaactgccc gacaatatgg acacgatgga
     9481 aggggcgctg gtggagcctg ccgcagtcgg gatgcatgcc gcgatgctgg cagatgttaa
     9541 accgggtaag aagataatta ttctgggagc aggttgtatt ggtttgatga cgttgcaagc
     9601 gtgcaaatgc ctgggcgcaa cggaaattgc cgtcgttgat gtactggaaa aacgtctggc
     9661 aataggggaa cagcctggtg cgacagtggt tattaatggc gcaaaagagg acactattgc
     9721 acgctgtcag caatttaccg aagacatggg cgcagatatt gttttcgaaa cagcgggttc
     9781 tgcggtcacc gttaaacagg cacctttaat ctggtaatgc gcggcggtaa aattatgatt
     9841 gttggtactg tacccggcga ttcggcaatc aatttcctca aaatcaatcg cgaagtcact
     9901 atccagacgg tattccgcta tgccaatcgt tatccggtca cgattgaagc tatttcttca
     9961 gggcgattcg atgtgaaatc gatggtgacg catatttacg attatcggga tgtacaacag
    10021 gcatttgaag agtcagttaa caacaaacgc gacattatta aaggcgttat taaaattagc
    10081 gattaattaa tacgggagaa cagaacatgc tcgcagatat cagatattgg gaaaacgatg
    10141 ccacaaataa atattatgca attgcccatt ttaacgtatg gaatgcagaa atgttgatgg
    10201 gcgttaaaga cgcagccgaa gaagcgaaat ccccggttat tatttctttt agtacaggtt
    10261 ttgtcggtaa cacctcattt gaagatttct ctcacatgat ggtatcaatg gcacaaaaag
    10321 caacggtgcc ggtaataact cattgggatc atggtcggag tattgagatt attcataacg
    10381 cctggactca tggtatgaat tcattaatgc gtgatgcttc tgcatttgat ttcgaagaaa
    10441 atattcgttt aaccaaagag gctgttgatt tctttcatcc gctgggtatt ccggtagagg
    10501 cggaattagg gcatgtcggt aatgaaaccg tttatgagga agcgctggcg ggctatcact
    10561 ataccgatcc tgaccaggcg gctgaatttg ttgaacgtac gggctgtgac tcattggctg
    10621 tcgccatcgg caaccagcat ggggtttata cgtcagagcc gcaattgaac tttgaggtcg
    10681 tcaaacgcgt acgcgatgct gtttctgttc cgctggtttt gcatggcgca tcggggatca
    10741 gtgatgccga cattaaaact gcaatttcgt tgggtattgc gaaaatcaac attcatacgg
    10801 agctctgtca ggccgcaatg gtagcagtga aagaaaacca ggatcagcct ttcctgcatt
    10861 tagaacgtga agtacgtaaa gctgtaaaag aacgggcatt ggaaaaaatt gaactgttcg
    10921 gttctgatgg caaagcggaa taatgatatg gataatctcg acgttatttg tataggtgcc
    10981 gctattgttg atattccatt acaaccggtc agtaaaaata tctttgatgt agattcttat
    11041 cctcttgaaa gaatcgcaat gagcaccggg ggcgatgcaa taaatgaagc aacaattatt
    11101 tctcgtctgg ggcatcgtac cgcattaatg agccgcattg gtaaagatgc ctcagggcaa
    11161 tttattctcg accattgtcg caaagagaat attgatattc aaagcctgaa acaggacgtc
    11221 agcatagata catctattaa cgttggactg gtgacggagg atggcgagcg gacgtttgtc
    11281 acgaaccgta acggcagttt gtggaaatta aatattgacg atgttgattt tgcgcggttt
    11341 cctcaggcga aattattatc actggccagt attttcaaca gtcctctatt ggatggtaaa
    11401 gcgctaacag aaatttttac gcaagcgaaa gcccggcaga tgatcatttg tgccgatatg
    11461 atcaaaccgc ggttgaatga aacgctggat gatatttgcg aagcattgag ttatgtcgat
    11521 tatctgtttc caaattttgc cgaggaaaaa ttactcacag ggaaagagac actggatgaa
    11581 attgctgact gctttcttgc gtgcggcgta aaaacggtgg tgattaaaac gggtaaagac
    11641 ggctgcttta tcaagcgtgg tgacatgacg atgaaggtgc cggcggtcgc aggaataacc
    11701 gccatcgaca ccattggcgc gggcgataac tttgcttcag gttttattgc ggcactgtta
    11761 gaaggcaaaa atctgcgtga atgcgcacgc tttgccaatg caacggcggc tatctcggtt
    11821 ctaagcgtcg gtgccaccac cggcgtaaaa aacagaaagc tggtcgaaca attgctggaa
    11881 gaatacgaag gataatgaag gcaaatgaaa aagatacctt taggcacgac ggatataacg
    11941 ctttcgcgaa tggggttggg gacatgggcc attggcggcg gtcctgcatg gaatggcgat
    12001 ctcgatcggc aaatatgtat tgatacgatt cttgaagccc atcgttgtgg cattaatctg
    12061 attgatactg cgctaggata taactttggc aatagtgaag ttatcgtcgg tcaggcgtta
    12121 aaaaaactgc cccgtgaaca ggttgtagta gaaaccaaat gcggcattgt ctgggaacga
    12181 aaaggaagtt tattcaacaa agttggcgat cggcagttgt ataaaaacct ttccccggaa
    12241 tctatccgcg aagaggtaga agccagcttg caacgtctgg gtattgatta catcgatatc
    12301 tatatgacgc actggcagtc ggtgccgcca ttttttacgc cgatagctga aactgtcgca
    12361 gtgcttaatg agttaaaggc tgaagggaaa attcgcgcta taggcgctgc taacgtcgat
    12421 gctgaccata tccgcgagta tctgcaatat ggtgaactgg atattattca ggcgaaatac
    12481 agtattctcg accgggcaat ggaaaacgaa ctgctgccgc tatgtcgtga taatggcatt
    12541 gtggttcagg tttattcccc gctagagcag ggattgttga ccggcactat cactcgtgat
    12601 tacgttccgg gcggcgctcg ggcaaataaa gtctggttcc agcgtgaaaa catgctgaaa
    12661 gtgattgata tgcttgaaca gtggcagcca ctttgtgctc gttatcagtg cacaattccc
    12721 actctggcac tggcgtggat attaaaacag agtgatttaa tctccattct tagtggggct
    12781 actgcaccgg aacaggtacg cgaaaatgtc gcggcactga atatcaactt atcggatgca
    12841 gacgcaacat tgatgaggga aatggcagag gccctggagc gttaaatact caacacgatt
    12901 cacctctgtt tctgtgaaaa ctgtgatgaa aatctgtgat tcaaataggt tattttgaaa
    12961 gtaaacatcg gttatgcgat aatcgcgcta atgtgatgta aaaagataca ggggtgtaat
    13021 cgtggcggca aaagacagga ttcaggcaat taagcaaatg gttgccaacg ataaaaaggt
    13081 gacagtctca aatttgagtg ggatttttca ggtaaccgaa gaaaccattc gccgcgatct
    13141 tgagaagctg gaagatgaag gctttttgac cagaacctat ggtggtgctg ttttaaatac
    13201 agcggtgttg acggagaata tccattttta taagcgcgct tcatcgtttt atgaagagaa
    13261 gctgcttatt gcacgcaagg cactaccctt tatcgacaat aaaaccacca tggcagccga
    13321 ttccagtagt accgtaatgg aattgctcaa attgttacag gaccgtagtg acctgacgtt
    13381 gctaaccaac tcggcggaag cgattcatgt tctggctcag tcagaaatta aagtcgtttc
    13441 aacgggtggg gaactaaaca aaaatacact ttccctgcaa ggaagaatta ctaaagagat
    13501 catcagccgc taccatgtcg atatcatggt aatgagttgt aaaggtcttg atattaacag
    13561 cggcgcgctg gactctaatg aagcggaagc tgaaatcaaa aagacaatga tccgtcaggc
    13621 gacagaagtt gcgttattgg ttgatcactc taagtttgat cgtaaagctt ttgtccagtt
    13681 agctgacttt agtcatatta attatataat aactgataaa tcaccgggtg cagagtggat
    13741 agcattttgc aaagacaata atatccagct ggtgtggtaa tttaagcaca cgttgtcaat
    13801 attatttgtt cctgcgactg gaaataaaat gatgataaag atgcggcaag tgaatttgcc
    13861 gcaattgcta ccttttatag caaatccgag gcacccaatg gaacaatatg atcaaattgg
    13921 cgcaagactg gaccgcttgc ctttggcccg gtttcattat cgtatatttg gtataataag
    13981 ctttagtctg ttattaacgg ggtttttgag ttactcaggt aatgtcgtct tagcaaagct
    14041 ggtaagcaat ggatggtcaa ataatttcct ctatgccgcc tttacctcgg cattaatgtt
    14101 tggttatttc atcggctcac ttactggtgg gtttattggt gactactttg ggcggcgcag
    14161 ggcgtttcgc ataaatcttc tcatcgtcgg tattgctgca acaggggccg cttttgtccc
    14221 tgatatgtac tggctcattt tctttcgctt cctgatggga acaggaatgg gggcgctgat
    14281 tatggttggc tatgcctcat ttacggagtt tatccccgcg acggtgcgtg gaaaatggtc
    14341 cgcgcggctc tcatttgttg gtaactggtc gcccatgctg tctgcggcga taggcgtggt
    14401 ggttatcgct ttttttagtt ggcgaataat gtttcttctg ggtggtattg gcatactgtt
    14461 agcctggttt ctctcaggta aatactttat cgagtcgcca cgatggctgg aagggaaagg
    14521 gcaaatcgcc ggtgcagaaa gccaacttcg tgaagtagag cagcaaattg aaagggagaa
    14581 gagtattcgt ttaccccagc ttactttgaa ccagagcaac agcaaggtta aggtaatcaa
    14641 gggtaccttc aggctcctgt ttaaagggga aatgttacga cgtacattag ttgcgataac
    14701 tgttttaatt gcaatgaaca tttcgcttta taccattacc gtatggatac cgaccatatt
    14761 tgttaactcc ggcattgatg tcgataaatc aatattaatg accgctgtta ttatgattgg
    14821 tgctccggta ggaatattta ttgcggcatt aattattgat cattttcctc gtcgattatt
    14881 tggctccgcc ttacttatta ttattgccgt gttaggctat atctattcaa gtcagactac
    14941 agagtgggcg attttaatct atggtctggt gatgatcttc tttttataca tgtatgtttg
    15001 cttcgcgtcg gcggtttata tcccggagct ttggccaacg catttacgcc tgcgcggttc
    15061 gggtttcgtt aatgccgtcg gacggatcgt cgcagttttc acgccctatg gcgttgcggc
    15121 attattaaca cattatgggt cgatcacggt gtttatggtg cttggtgtca tgttagtgct
    15181 ctgtgcgctg gttctctcca tttttggcat cgaaacgcgg aaggtgtcgc tggaagagat
    15241 ttctgaggtg aattaaatct ttcgttgatg gcatcaattg ccagatgtac cactgacgtg
    15301 ttttatttgg cctacaatgc cagtacagaa ttgtaggcca gcgtaaaatt acccctgcgt
    15361 ctcttcccag tctgccagcg tatatagcgt tgccccagct gctgacatct ccataaacgc
    15421 gtgtgcactg tcctggggct ggatattcac gccacgacag ccatcggtaa tcacgtttac
    15481 cttataacct aactgtaacg cgtccagcac ggtaaacttc acgcaatagt cagtagccag
    15541 gcccataacg atcaattcat cgatttcatg atcgcgcaac cagtcatcaa gcgcggtttt
    15601 ctgccgacgt ccgttatcaa aaaaggcact gtaactgtca actaaaggat tttcgccttt
    15661 atggaacacc gctgcgatct ctttttgatt cagtaacgga tgtaattgtg cgccttcact
    15721 gttctgcaca cagtgttcag gccagaaagt ttgccgcagg ccgccgagtt ggcctggcgt
    15781 ataaggctct acgccgtgct ggctggcaaa gctgccgtga ttcgccggat gccagtcctg
    15841 gctggcgata accgcttctc cgcgcgactg gcaccagtca ataaggcggt tagcaacctc
    15901 caccgtactg tcaccttccg gtacggcgag cgcgccacca gcacagaaat cattttgtaa
    15961 atcgaccagt aacagggcgc gatggggcat tgcagtctcc ttaatcatcc ggcgtcagtt
    16021 cgccgcgcag gttttggctc atggccttgc gaatggtttc agtatccagt tcctggctca
    16081 gcaggtaatg cagtttggtt agcgtggctt ctacagtcat atctgcaccg tcaataacgc
    16141 cggcatgggc gagggcgtta ccggtggcat aaccactcat attcacttta ccggacatac
    16201 attgtgtcag gttgaccacc acaataccgc gatcgctggc ttcttgtaat tcctgcagga
    16261 aggctttgtt ttgtggcgca ttacccacgc cataggagcg cagaatcaat gctttcaccg
    16321 gttggcgcag aaaattgcgc acgacgtcag cagaaatccc tggataaatc gtcaccacgc
    16381 caattggttg tggcgtgatt ggatgaacga ttaattcccc ttcaccgtgc ggggcgggtg
    16441 gcgtattcaa acgacgaata tggataccag cttccagtaa cggaggaagg tttggagagg
    16501 caaacgcatc aaaaccatcg gcatgggctt tggtagtgcg gttgccgcga tacaatcggt
    16561 tattgaaaaa gagcgttact tcgttgatcg gataattcgg cgcaacgtac aacgcattca
    16621 gcagattaat ttgtccgtca gagcgtaact cagccagcgg gatttgtgac cctgtcacaa
    16681 tgaccggttt accgagattc tcgagcatga acgacagcgc agaggcggta tacgccatcg
    16741 tgtcggtacc gtgtagaatg acaaaaccat catagtcgtc atagtgcgct ttaatatctt
    16801 cagcaatatg ctgccagtct tccggcgtca tatcagaaga atccatcaac ggcgtatatt
    16861 catgaatggt gaaatctggc atctccgggc gatggaattc cggcatcagc gccagttggc
    16921 gttgtagatg acctgacacc ggaatataac cctgctcgga acgctgcatc ccgatggtcc
    16981 cgcccgtgta ggcgacgtaa attgatttct tttgcatgat attgatatga atgatatcga
    17041 aagagcaaaa gtatatacgt gaggcaggag gagcgcacct gatactatgc ggataaggaa
    17101 atatggggcg agacagcggg aagaggattc gtcgaggtaa aaaataatgt ggccgacgat
    17161 ctgtcggcca ctcagtacaa gacttaacgc acgttggcgc aggtcaggca aaacgcataa
    17221 cggttttgtg ggtcgttaaa cgcggccagc ttgtcgcttt cacttttaac ggtagaggct
    17281 accgaggcaa gcggtgcagg taacatggcc tggaacgcat ctggcaacat tgcccggaca
    17341 gaaccagaca tgttgtccat cactttgtcg aagaaggtcg gttcatcaac gtagtattcc
    17401 agatgccact gtttcacttt tgcgagctct gctgctttgg caaccgcatc atcgaaatcc
    17461 ccgagactat cgaccagccc gttagctttt gcatcctgac cggtccagac gtggccctgg
    17521 gcgattttat caatttgctc cggcgtcgaa tgacgcgcat cagcaaccag cgtgataaag
    17581 cgtttatagc cattctcaat gcttaattgc atcatctgct gcgcttccgg cggcagtgcc
    17641 ctggtgatag aaacatccgc cagcggtgaa gttgagacac catcagtatg aacaccaatc
    17701 gaatccagac tattttctac ggtggtgatc acgccgaaga taccgataga accggtcagg
    17761 gtgctggggt tagccacaat gtaattagct ggcgtggaaa tccagtaacc accagatgcc
    17821 gccatgccgc ccatcgatac aaccacagac ttacccgctg cccgggctgc tgccagttca
    17881 gcgcgaatca cttcagacgc ggtaacgctg ccgcctgggc tattaacacg caggacaatc
    17941 gctttcactt tcgggtcaag gcgagcgtcg cggatttgtg ccgcagtggt atcaccgcca
    18001 acattcccct gagtttcctc gccatccata attgcgccat tagcgaagac gacaccgatg
    18061 ctgtcaccgg tatctgccgg cgttttcaat gcgtaatcgt aataactgat ggcgcgataa
    18121 tttttatcag tcttactcca gccgaactct ttggtcagag ctttttcgat ttcggcactc
    18181 gatgccagtg catcgaccag cttgttttcc agtgcatatt tcgcggtatc gccaccggtt
    18241 ttggttaaac cctcaagcaa cccttgcgcg ccagggaata cctgctgagc agggatctgc
    18301 cggttagcgg caacagtatt cagatagttt tgccacagct caccaatcca gcggctgtca
    18361 gcttcgcggg ctgccggtga catatcatca cgaataaacg gttcaacggc agatttatac
    18421 gtacccacgc ggaacacatg ggtggaaact ttcagcttat ccagcaacga tttgtagtac
    18481 agaccgttgg tagcaaaacc gtgcagatca accacgcctt gcggtgacag ccaaatttta
    18541 ttggcgaaac tggcgagata atattgcccc tggctgtagt tctcgccaac ggcataaacc
    18601 ggtttcccgc tgtcacgaaa ctctttcagg gctttgccga tgtactgcat agacggctgg
    18661 tcgccgcctg cgaagttttt cagatccatc acgataccgg tgatattgcg gtcgtccttc
    18721 gcctggcgaa tggtgttgac gatatcaaac agtgagtttt cctgcagacg atcggaactg
    18781 gcaccaagca gctggcggct taatttacta aaccgctgag aactgtctgg tttatcgacg
    18841 atcacaccag aaatgtccag cagcagtgcg ccacgactgg ccgtttcttt cgaatcacca
    18901 ccactgacct gcatccagat ccccacacca accagtacga ggaaaataaa gaacaggtta
    18961 agtaccattt cacggacgaa attcagcaga cgccacgtcc atttaaaaaa tccggcaata
    19021 aatcgccaaa gggttcgcat gtattctccc aacttaaggt cacacctgtc acaggcgcaa
    19081 tagatgtggt tatcgtaatg acccaaccgg cacttgtcag caggaattgc ggactgtgct
    19141 gtaacaaaat ccactctcgt gttaattttg tgaataaata tcacgacagg agttaatcaa
    19201 atggatgcac tcgaactatt gatcaatcgc cgtagcgcct cccgcctggc tgaacccgcg
    19261 ccaacgggtg aacaactgca aaacatcctg cgtgcgggta tgcgtgcgcc ggaccataag
    19321 tccatgcaac cgtggcattt ttttgtgatt gaaggggaag ggtgcgagcg tttcagcgcc
    19381 gtactggaac agggggcgat tgctgccggt agtgatgata aagctatcga caaagcccgt
    19441 aatgcgccgt tccgcgcacc gctcatcatc acggtggtgg cgaaatgcaa agaaaatcat
    19501 aaagtcccgc gatgggaaca ggaaatgtct gccggatgcg cggtcatggc gatgcaaatg
    19561 gcagccgttg cccagggatt tggcggcatc tggcgcagtg gcgcattaac tgaaagtccg
    19621 gtagtgcgtg aagcattcgg ttgccgtgag caggataaaa ttgtcggttt tctctacctc
    19681 ggcacgccgc agttgaaagc atctacgtcg attaacgtcc cggacccgac gccgtttgta
    19741 acttatttct gataattgtc gcgaaactgt ctggattgtg agcaaaggcg caggaattca
    19801 gacactctca cttatcactt cacggaatga gggttaccat acccggattg cattgacagg
    19861 agatgtccat gagcgagaac tcgattcgtt tgacccaata cagccacgga gctggttgcg
    19921 gctgtaaaat ttccccaaaa gtgttggaaa ccatcctgca cagtgagcag gcgaagtttg
    19981 ttgatccgaa tttgcttgtg ggtaatgaaa cccgcgacga tgcggcggtg tacgatctgg
    20041 gcaatggcac cagcgttatc agtaccaccg acttctttat gccgatcgtt gataatcctt
    20101 tcgattttgg ccgcattgcg gcgactaacg ccatcagcga tatcttcgcg atggggggca
    20161 aaccgattat ggcgattgcg atcctcggct ggccgattaa caaactttcc ccggaaattg
    20221 cccgcgaagt aaccgaaggt ggacgctatg catgccgtca ggcgggtatt gcgctggctg
    20281 gcggtcactc catcgatgcg ccggagccga tttttggtct ggcggtaacg gggatcgtac
    20341 cgaccgagcg ggtgaagaaa aacagtaccg cacaagccgg atgcaaactg ttcctgacga
    20401 aaccgctggg gatcggcgtt cttaccacgg ctgagaaaaa atcactgttg aaaccagaac
    20461 atcagggact ggcgacggaa gtgatgtgcc ggatgaacat cgcaggcgcg tcctttgcca
    20521 acatcgaagg cgtaaaagcg atgaccgacg ttacgggctt tggtctgctg ggccacttga
    20581 gcgaaatgtg tcagggggct ggtgtgcagg cacgcgtcga ctatgaagcg atcccgaaac
    20641 tccccggtgt tgaagagtac attaagttgg gcgcagtacc tggcggcact gaacgtaact
    20701 ttgccagcta cggtcatctg atgggtgaaa tgccgcgtga agtgcgcgat ctgctgtgcg
    20761 atccgcaaac ttctggcggt ttgctgctgg cggtcatgcc ggaagcagaa aatgaggtta
    20821 aaactacagc cgccgagttt ggcattgaac tgacggcgat tggcgaactg gtgccagcgc
    20881 gcggcggtcg tgccatggtt gagatccgtt aatttaatgc ggttgtttat tgccgaaaaa
    20941 ccgagtctgg cgcgcgccat tgctgatgtc ctgcccaaac cgcaccggaa aggcgatggc
    21001 tttatcgagt gcggtaatgg tcaggtggtg acttggtgta tcggtcacct gcttgagcag
    21061 gcgcagccag acgcctacga cagccgctat gcgcgctgga atcttgcgga tttgccgatt
    21121 gtcccggaaa agtggcaatt acagccacga ccctccgtga ccaaacaact taacgtcatc
    21181 aaacgcttcc tgcatgaagc cagcgaaatc gttcacgccg gggacccgga tcgtgaaggg
    21241 caattgctgg tggatgaagt gctggactat ctgcaactgg caccggaaaa gcgccagcag
    21301 gtgcagcgtt gcttgataaa cgacctgaac ccgcaggcgg ttgagcgggc gatcgaccgt
    21361 cttcgttcca acagtgagtt tgtgccattg tgcgtttctg cgctggcgcg agcgcgtgcc
    21421 gactggctgt acggcatcaa tatgacccgt gcgtatacca ttctcggtcg caatgccggt
    21481 tatcagggcg tactttccgt gggacgcgtg cagacacccg tgctcgggct ggtggtgcgc
    21541 cgcgatgaag agattgaaaa cttcgtggcg aaagacttct ttgaagtcaa agcacatatc
    21601 gtgacacctg ccgatgagcg gtttaccgct atctggcaac cgagcgaagc gtgtgaaccg
    21661 taccaggatg aagaagggcg cttgttacat cgtccactgg cggagcatgt ggttaaccgc
    21721 attagtggtc aaccggctat tgtcaccagc tataacgata aacgggaatc agaatccgcg
    21781 ccgctgccgt tttcgctttc ggcgttgcag attgaagcgg caaaacgctt cggtctgagc
    21841 gcgcagaacg tgcttgatat ctgccagaag ctgtacgaaa cgcacaagct aatcacttat
    21901 ccgcgttccg attgtcgcta tttgccagaa gaacattttg ccggacgcca cgcggtgatg
    21961 aatgccatca gcgttcatgc accggatctg ttgccgcagc cagtggtaga tccagatata
    22021 cgcaaccgct gttgggatga caaaaaggtc gatgcgcacc acgccatcat cccgaccgca
    22081 cggagttctg cgatcaacct gacggagaac gaagcgaagg tctataacct gattgcccgt
    22141 caatacctga tgcagttctg cccggatgcg gtgttccgca agtgtgttat cgaactggac
    22201 attgccaaag gcaaatttgt cgctaaagcc cgttttcttg ctgaagcagg ctggcgcacg
    22261 ctgttaggca gcaaagagcg cgatgaagaa aacgacggca cgccattgcc tgtggtggcg
    22321 aaaggcgatg agttgctgtg tgaaaaaggt gaagtggtag agcggcaaac ccagccgccg
    22381 cgccatttta ccgatgcaac actgctttcg gcgatgaccg ggatcgcgcg ctttgtgcag
    22441 gacaaagatc tgaaaaaggt ccttcgtgcg accgatggtc tggggacaga agcaacccgt
    22501 gccgggatta ttgaactgct gttcaagcgt ggttttctga ccaaaaaagg gcgctatatc
    22561 cactccaccg acgccggaaa agcgttattc cattcgctgc cagaaatggc gacgcgaccg
    22621 gacatgaccg cgcactggga atcggtgctg acgcaaatca gcgaaaagca gtgtcgctat
    22681 caggacttta tgcagccgct ggtggggacg ctatatcagc ttattgatca agccaaacgt
    22741 acgccggtgc ggcagtttcg cggcattgtg gctccgggca gtggtggcag tgctgataag
    22801 aaaaaggctg caccgcgtaa acgtagtgcg aaaaaaagtc cgccagcaga tgaagccgga
    22861 agcggggcga tagcgtaagc gagcgaatct ttcgtgctat tcgagtcata ttctgaaata
    22921 tccagcggat caagaaagtt cgttggatat ttttttgcat ggataaaatt atcgcctcta
    22981 aagtatgtaa taacagggaa tgtgatgaaa aaagttttat tgcaaaacca tcctgggagc
    23041 gagaagtatt cttttaatgg ctggggaata tttaatagta attttgaacg ggtgattaaa
    23101 gaaaataagg ccatgctgct ttgtaagtgg gggttttatt taacatgtgt tgtcgctgta
    23161 atgtttgtat tcgcagcgat aacatccaac ggtttgaatg aaagaggcct gattaccgcg
    23221 ggatgctctt ttctttatct attaattatg atggggctta ttgttcgggc cggttttaaa
    23281 gcaaaaaaag aacaactgca ttattatcag gctaaaggta ttgagccgct cagtatcgag
    23341 aagttacagg cgctacaatt gatcgcacct tatcgattct atcataagca atggtctgaa
    23401 acgctagagt tctggccgcg aaagcctgaa cctggcaaag ataccttcca atatcatgtg
    23461 cttccttttg actcgatcga tatcatcagt aaaagacgcg agtctttaga gaatcaatgg
    23521 ggtatcgaag atagcgaaag ttattgtgcc ttaatggagc attttctttc tggcgaccat
    23581 ggagccaata cctttaaagc aaacatggag gaagccccag agcaggttat cgccttgttg
    23641 aataaatttg ctgtttttcc ctcagactat atctctgatt gcgctaataa tcgctccggt
    23701 aaatcctcgg cgaagctaat atgggcggcg gaattatcat ggatgatctc gatatcaagc
    23761 acagcttttc aaaacgggac aattgaagaa gaactggcct ggcattatat aatgcttgct
    23821 tctcgaaagg cgcacgagtt gttcgaaagc gaagaagatt atcaaaaaaa tagtctaatg
    23881 ggatttcttt actggcatat ctgctgctat cgcagaaagt taacggatgc tgaacttgaa
    23941 gcgtgttatc gttacgacaa gcagttttgg gagcactaca gtaagaaatg ccgttggccc
    24001 ataagaaatg ttccctgggg agcatcatcc gttaaatact cataaacgcc tgaaattttg
    24061 ccgggggcgc tgcgcttgac cggcctacat tagagcgtaa ctgcaatgaa ttgtgcccat
    24121 ctgtaggcct gataagcgta gcgccatcag gcatttacaa cttaaatcac accctgcgcc
    24181 agcatcgcat cggcaacctt cacaaaaccg gcaatgttcg cgccctgcac gtagttggtt
    24241 tgctcacctt caccaccatg ctcaacacag gcatggtgga tatccagcat gatgtgatgc
    24301 aaacgtgcgt caactttctc ggctttccag cccaggcgcg cagcgttttg tgccatttcc
    24361 aggcccgacg ttgctacacc gccagcatta gccgctttac ccggtgcaaa cagtacgcct
    24421 gcctgctgga acagttcagt cgcttcgatg gtggtcggca tatatgcccc ttcagcgacg
    24481 gctttaacgc cattggcgat aagctgatgc gcggcgtcaa catccagttc gttctgggtg
    24541 gcgcaaggca gggcgatatc aaccggtaca gaccacggct gttggccttc gagatagacc
    24601 agaccaaatt ctttggcgta atctgccgct cgaccatcgc ggctggcttt gatttcgata
    24661 agacgtgcca gtttctcttt cgtgaatccg ctttcatcaa ctacagtgcc gctggagtct
    24721 gacgcagtga tcacacgagc accaaattcc atcgcttttt cgatagcgta ctgggcgacg
    24781 ttgccggagc cagaaacgga aacgctcatc ccttcaaaac ccataccgtg gcgttttaac
    24841 attgcttctg tgaaataaac cagaccgtag ccggtagctt ccgggcgaat aagactaccg
    24901 ccaaatgaaa ggcccttacc ggtgaagacg caggcggtat tgttggagag ctttttcatc
    24961 atccccgcca taaagccgac ttcacgacca ccaacgccga tatcacctgc cggaacgtcg
    25021 gtatccgcgc ccaggtggcg atacagttca gtcatcagcg tctggcaaaa acgcatcact
    25081 ttaccttcgc tttttccttt cggatcgaaa tcgctgccgc ctttaccacc gcccatcggc
    25141 agggtagtca gggcattttt gaaggtttgt tcaaagccga ggaatttgag aatggaaag
//
LOCUS       DAJTUP010000056        24596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTUP010000056 DAJTUP010000000
VERSION     DAJTUP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.056"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(2..94)
                     /locus_tag="OO468_002322"
     tRNA            complement(2..94)
                     /locus_tag="OO468_002322"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO468_002323"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO468_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR6259412.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO468_002324"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO468_002324"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR6259413.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO468_002325"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO468_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR6259414.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO468_002326"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO468_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR6259415.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO468_002327"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO468_002327"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR6259416.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO468_002328"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO468_002328"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR6259417.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO468_002329"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO468_002329"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO468_002330"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO468_002330"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR6259418.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO468_002331"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO468_002331"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR6259419.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO468_002332"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO468_002332"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR6259420.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO468_002333"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO468_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR6259421.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO468_002334"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO468_002334"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR6259422.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO468_002335"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO468_002335"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR6259423.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO468_002336"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO468_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR6259424.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO468_002337"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO468_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR6259425.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO468_002338"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO468_002338"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR6259426.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO468_002339"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO468_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR6259427.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO468_002340"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO468_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR6259428.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO468_002341"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO468_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR6259429.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO468_002342"
     CDS             complement(20900..21019)
                     /locus_tag="OO468_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR6259430.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO468_002343"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO468_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR6259431.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO468_002344"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO468_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR6259432.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..>24596)
                     /locus_tag="OO468_002345"
     CDS             complement(23682..>24596)
                     /locus_tag="OO468_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6259433.1"
                     /translation="AGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWF
                     RTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGA
                     FVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVA
                     LAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICK
                     GDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQ
                     CEGSALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT         5800 a         6922 c         6309 g         5565 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagc
//
LOCUS       DAJTUP010000057        24341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTUP010000057 DAJTUP010000000
VERSION     DAJTUP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.057"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            137..1240
                     /gene="proB"
                     /locus_tag="OO468_002346"
     CDS             137..1240
                     /gene="proB"
                     /locus_tag="OO468_002346"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR6259434.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1252..2505
                     /gene="proA"
                     /locus_tag="OO468_002347"
     CDS             1252..2505
                     /gene="proA"
                     /locus_tag="OO468_002347"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR6259435.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2620..2695
                     /locus_tag="OO468_002348"
     tRNA            2620..2695
                     /locus_tag="OO468_002348"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2653..2655,aa:Thr,seq:cgt)
     gene            complement(2710..3870)
                     /locus_tag="OO468_002349"
     CDS             complement(2710..3870)
                     /locus_tag="OO468_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6259436.1"
                     /translation="MSIKLRGGTWHCDFVAPDGSRVRRSLETSDKRQAQELHDRLKAE
                     AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWMLHFREAILSDITTEK
                     IMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTKTRHLAILRAILNMA
                     VEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPVVMFAITTGLRRSNV
                     TDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQGLHKRWVFVHTKPA
                     YRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWASWLVQSGVSLLALK
                     EMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEENVVYLNAR"
     gene            complement(3762..4094)
                     /locus_tag="OO468_002350"
                     /pseudo
     CDS             complement(3762..4094)
                     /locus_tag="OO468_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(4181..4525)
                     /locus_tag="OO468_002351"
     CDS             complement(4181..4525)
                     /locus_tag="OO468_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259437.1"
                     /translation="MSDLAMKVLKWQSTDDVGISSATLASIACGLKKNIYGHSFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            complement(4626..4805)
                     /locus_tag="OO468_002352"
     CDS             complement(4626..4805)
                     /locus_tag="OO468_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Eag protein"
                     /protein_id="HCR6259438.1"
                     /translation="MSCPKCGSGNIAKEKTMRGWSGDYVCCDCGYNDSKDAFGERGKN
                     EFVKINKEREGNEKS"
     gene            complement(4902..5831)
                     /locus_tag="OO468_002353"
     CDS             complement(4902..5831)
                     /locus_tag="OO468_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001609777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6259439.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVAY
                     IIQDSDARSRGEKGILRYFANISDEDINEYEITVTPLYAAPPETVSVPDAMEMDDDFD
                     SAFEHGKAVGWNAYRAAMLQSGNFRENKNSSTNNFREIAETSTNYPVIPSEVLSAIQK
                     VAKIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLAAEPIAPNDVREQTAIPQ
                     VPVTPDGWISCSERMPVIGELNWRTSFPLLITCEIGVIPAYYGFVSVNGNKHYGFMES
                     LKYGDDNGNHPQTNEYGLISNVTHWMPLPEPPL"
     gene            complement(5834..6025)
                     /locus_tag="OO468_002354"
     CDS             complement(5834..6025)
                     /locus_tag="OO468_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259440.1"
                     /translation="MDGQISIVRPGACDDREIRLIIRLAMGKTITALITPENLALALT
                     GKSDLPVELKLRNIEIKVK"
     gene            complement(6027..6656)
                     /locus_tag="OO468_002355"
     CDS             complement(6027..6656)
                     /locus_tag="OO468_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR6259441.1"
                     /translation="MSEINYQALREAAERAIPAMERLLMLPADDLLSEQELKDYGVDI
                     DALNAFKFLAGPETVLALLDERVRNQQYIKSRDQENEDIALTVGKLLIENGRLVADTL
                     RHLADNEIDSDYFAITSTNENGTEIDHEMAITDYALQAAGTVDELVAALESAENRIAE
                     LGERGILLPERSSMLHRTDFHDDYQTVMAYKVSEVIAAIRAAGIRIKGE"
     gene            complement(6653..7228)
                     /locus_tag="OO468_002356"
     CDS             complement(6653..7228)
                     /locus_tag="OO468_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hNH endonuclease"
                     /protein_id="HCR6259442.1"
                     /translation="MKQMSLIEMDGFLKGKCIPCDLKVNETNAEYLVRKFAEAEAKIS
                     ALYEDHQKAIESIKQADAAVKLAHEKFSALAAENAGMKSVIEYCINPDNQPEYHDQGM
                     GCGVEDHGYQRDGYSACYYGWESAMDRVYSEVIPDAIPETPATDTFLDEVRAQGVEMF
                     AASMKVVGGHEHPYSAMANEFAAQLRKGGGQ"
     gene            complement(7225..7389)
                     /locus_tag="OO468_002357"
     CDS             complement(7225..7389)
                     /locus_tag="OO468_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HCR6259443.1"
                     /translation="MRGLAYNPGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKY
                     LNAMWRSGKK"
     gene            complement(7400..7693)
                     /locus_tag="OO468_002358"
     CDS             complement(7400..7693)
                     /locus_tag="OO468_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HCR6259444.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEELERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(7717..8100)
                     /locus_tag="OO468_002359"
     CDS             complement(7717..8100)
                     /locus_tag="OO468_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259445.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVVFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSQQQQSSNHQNHPEYDDSIPF"
     gene            complement(8100..8705)
                     /locus_tag="OO468_002360"
     CDS             complement(8100..8705)
                     /locus_tag="OO468_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HCR6259446.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAALQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDIYNIRRDELEGAAA"
     gene            complement(8962..9114)
                     /gene="kil"
                     /locus_tag="OO468_002361"
     CDS             complement(8962..9114)
                     /gene="kil"
                     /locus_tag="OO468_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HCR6259447.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINI
                     PWYRRK"
     gene            complement(9099..9233)
                     /locus_tag="OO468_002362"
     CDS             complement(9099..9233)
                     /locus_tag="OO468_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259448.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLISAAKKQPEALCAMK
                     "
     gene            complement(9428..9898)
                     /locus_tag="OO468_002363"
     CDS             complement(9428..9898)
                     /locus_tag="OO468_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259449.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQKRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(9907..10233)
                     /locus_tag="OO468_002364"
     CDS             complement(9907..10233)
                     /locus_tag="OO468_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HCR6259450.1"
                     /translation="MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKP
                     KSRVESALNPIDLTVLAEYHEQIESNLQRIERKNQRTWYSKPRSEMGVTCVGRQKMKL
                     GSKPLI"
     gene            complement(10597..11001)
                     /locus_tag="OO468_002365"
                     /pseudo
     CDS             complement(10597..11001)
                     /locus_tag="OO468_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000618033.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10998..11630)
                     /locus_tag="OO468_002366"
     CDS             complement(10998..11630)
                     /locus_tag="OO468_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HCR6259451.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGDGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            11734..11949
                     /locus_tag="OO468_002367"
     CDS             11734..11949
                     /locus_tag="OO468_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6259452.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            12066..12347
                     /locus_tag="OO468_002368"
     CDS             12066..12347
                     /locus_tag="OO468_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015995142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259453.1"
                     /translation="MGTIATKSKKAARIESALLNKLALMGQKTFARAMGVPEYQVSRW
                     KNGFFSQVSMMLAVLEYGIEDDEMAELTKRLADYLTKEKAPKNGEFFEA"
     gene            12382..12543
                     /locus_tag="OO468_002369"
     CDS             12382..12543
                     /locus_tag="OO468_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259454.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            12530..13360
                     /locus_tag="OO468_002370"
     CDS             12530..13360
                     /locus_tag="OO468_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015980178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR6259455.1"
                     /translation="MGVVKLADYRPQLEVVEHRVAELEDGYTRTANTLLEAAMLSGLT
                     LHQLLIVMAVWRKTYGYNKKIDWIGNEQFAELTGMAPTKCSTAKNELIRMGVLIQVGR
                     QVGMNTNISEWKTKVNGFGKTFTNSVKQTFTKSVKSNLPNQSNTKDNIQKTINTNTPL
                     PPNGGGDGQVKPERRKAERIDYESFLNAYNTEVGDRLPHAVAVNEKRKRRLKKIIPQL
                     KTPNVDGFRAYVRAFVHQAKPFYFGDNDTGWTADFDYLLREDSLTGVREGKFADRGIA
                     "
     gene            13357..14733
                     /locus_tag="OO468_002371"
     CDS             13357..14733
                     /locus_tag="OO468_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6259456.1"
                     /translation="MKQDIEASVIGGLLIGGLTPTASDVLATLEPEAFSIPLYRKAFE
                     VIRKQARNRNLIDALMVAEACGEEHFTSILMTSKNCPSAANLKGYAGMVADNYHRRLV
                     LEIMDEMREPIQSGTIDASSQAMDELVKRLSAIRKPRDEVKPVRLGEIITDYTDTLDR
                     RLRNGEESDTLKTGIDELDAITGGMNAEDLVIIAARPGMGKTELALKIAEGVASRVIP
                     GSDVRRGVLIFSMEMSALQIAERSIANAGRMSVSVLRNPASMDDEGWARVANGMSQLA
                     DLDVWVVDASRLSVEEIRSIAERHKQENPNLSLIMADYLGLIEKPKADRNDLAIAHIS
                     GSLKAMAKDLKTPVISLSQLSRDVEKRPNKRPTNADLRDSGSIEQDADSIIMLYREAV
                     YDENSSAAPFAEIIVTKNRFGSLGTVYQRFCNGHFVACDQDEARQICTASNAPAARGR
                     RYAQGADV"
     gene            14806..15132
                     /locus_tag="OO468_002372"
     CDS             14806..15132
                     /locus_tag="OO468_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259457.1"
                     /translation="MADWQIPIIILAGASLVAGFILLKKHKDRDQKVEVLYGYPANST
                     TWLTIYHYRKSGRWVFEWDDLFAEKRPKSWGDISECMMFEERKSGATREEFNEAWARL
                     SERGYL"
     gene            15129..15329
                     /locus_tag="OO468_002373"
     CDS             15129..15329
                     /locus_tag="OO468_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259458.1"
                     /translation="MSKYEKLDQNILSMLSERPTPVFDIWLKWRSNGMYIETIDRRMQ
                     YLRKKGLVANVRGKGWVKINLS"
     gene            15341..15529
                     /locus_tag="OO468_002374"
     CDS             15341..15529
                     /locus_tag="OO468_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004904153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259459.1"
                     /translation="MDDSLEIFEEWCADELGVSAYFIRQMRSKNILGVIEYKRVEINK
                     RYRAWMAAVRAAGVKVKE"
     gene            15532..15696
                     /locus_tag="OO468_002375"
     CDS             15532..15696
                     /locus_tag="OO468_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259460.1"
                     /translation="MSAYEEIMLALRFFFGVEEDENVNEIIGQDHDPIGTIAAALDDY
                     RSVNGEETNV"
     gene            15677..16117
                     /locus_tag="OO468_002376"
     CDS             15677..16117
                     /locus_tag="OO468_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HCR6259461.1"
                     /translation="MRKLTFELRSPIHQQNAIQAIQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGRSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDKTE"
     gene            16114..16704
                     /locus_tag="OO468_002377"
     CDS             16114..16704
                     /locus_tag="OO468_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine-binding protein"
                     /protein_id="HCR6259462.1"
                     /translation="MNKYRLIYADPPWQYRDKANDGNRGAGHKYDVMNVQDICRLPVW
                     DLADPESCLLAMWWVPTQPAEALKVIEAWGFRLMTMKGFTWHKTNKHKGNSAIGMGHM
                     TRANSEDCLFAVRGRLPERMDASICQHFTAPRMEHSAKPPIVRDMLAKLLGDVPRCEL
                     FSRDKVTGWDMWGNQCDSDFELAPGMAIKPCKMVIA"
     gene            16701..16874
                     /locus_tag="OO468_002378"
     CDS             16701..16874
                     /locus_tag="OO468_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninD"
                     /protein_id="HCR6259463.1"
                     /translation="MKHCYRCGERKEDDRFRPGQPYWNRWCLRCERTPTGVLPLPQEK
                     EDVWRDSDEVSPT"
     gene            16841..17016
                     /locus_tag="OO468_002379"
                     /pseudo
     CDS             16841..17016
                     /locus_tag="OO468_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            17019..17420
                     /locus_tag="OO468_002380"
     CDS             17019..17420
                     /locus_tag="OO468_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259464.1"
                     /translation="MLLIQPGFGLSIKKGHMFGEKESQRKMMSIRLPFISIYWLNREA
                     TNYWYTCARAAFNDPDWFVENHHAVRQAKRKANMTYMKAYRKAWKEHRDRYQQDMEKL
                     ESENMELRRKLGEAKRDIDAYKRLFNGESHA"
     gene            17380..17610
                     /locus_tag="OO468_002381"
     CDS             17380..17610
                     /locus_tag="OO468_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HCR6259465.1"
                     /translation="MLTSDFLMVKAMLSPSQSLQYQKESVERALTCANCGQKLHVLEV
                     HVCEHCCAELMSDPNSSMYEEEDDGQTSAKTM"
     gene            17582..18193
                     /locus_tag="OO468_002382"
     CDS             17582..18193
                     /locus_tag="OO468_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HCR6259466.1"
                     /translation="MAKPARRRCKNEECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVVECKAIKAEYQQKLKDLRNSRSEAA"
     gene            18190..18396
                     /locus_tag="OO468_002383"
     CDS             18190..18396
                     /locus_tag="OO468_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HCR6259467.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            18374..19039
                     /locus_tag="OO468_002384"
     CDS             18374..19039
                     /locus_tag="OO468_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCR6259468.1"
                     /translation="MRYYEKIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NIDYDKEILAKALSHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            19036..19659
                     /locus_tag="OO468_002385"
     CDS             19036..19659
                     /locus_tag="OO468_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6259469.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDIAKTEQWGRVVEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAVTR"
     gene            20092..20415
                     /locus_tag="OO468_002386"
     CDS             20092..20415
                     /locus_tag="OO468_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HCR6259470.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            20399..20875
                     /locus_tag="OO468_002387"
     CDS             20399..20875
                     /locus_tag="OO468_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR6259471.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            20872..21309
                     /locus_tag="OO468_002388"
     CDS             20872..21309
                     /locus_tag="OO468_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6259472.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIRTQCLK"
     gene            21514..22056
                     /locus_tag="OO468_002389"
     CDS             21514..22056
                     /locus_tag="OO468_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6259473.1"
                     /translation="MNYPTVVNDIDFRDLIFVANNDPVTDSFMVAKAFGKLPKNVVRD
                     IERTIEACPPEFDTKLNFELCYKNNELQNGKPQKFYRLRKDGLMLLVMSYTKKEAMRI
                     KIAYINAFNWMYAMLQVGHRQFEEERNAVMLEYMKEKDVASMSGRLLNRWGKIKKPQL
                     MARIERLEQHGQTVIPGLIN"
     gene            22276..22491
                     /locus_tag="OO468_002390"
     CDS             22276..22491
                     /locus_tag="OO468_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6259474.1"
                     /translation="MDDNGNQWVNVEHRLPESKEGMWSKEVIALTDTGDVFKLSCMGS
                     YWQRTKAFIDSGASKVTHWMPLNYPDD"
     gene            22639..22881
                     /locus_tag="OO468_002391"
     CDS             22639..22881
                     /locus_tag="OO468_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2560 family protein"
                     /protein_id="HCR6259475.1"
                     /translation="MAEIIPMTEEQKFQLEIYKLVMNQNAAAEEAFQFIGTDELKLEL
                     FKIHFQSGGANSDITIRTFEAVRKSKEALDLFTTGA"
     gene            22917..23405
                     /locus_tag="OO468_002392"
     CDS             22917..23405
                     /locus_tag="OO468_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR6259476.1"
                     /translation="MAAPKGNRFWEARSSHGRNPKFESPEALWAACCEYFEWADDNPL
                     WEGKVFSYQGEIIKANVPKMRAMTISGLCTFLDITRQTWGTFRSMEGFSDVTSRAEDI
                     IYDQKFSGAAADLLNANIIARDLGLKEQSQVEDVTPDKGDRDKRRSRIKELFNRGTGR
                     DS"
     gene            23383..>24341
                     /locus_tag="OO468_002393"
     CDS             23383..>24341
                     /locus_tag="OO468_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR6259477.1"
                     /translation="MELDAILDNLSDEEQIELLELLEEEENYRNTHLLYEFTPYSKQR
                     EFIDAGHDYPERCFMAGNQLGKSFTGAAEVAFHLTGRYPGTKGYPADGKYGGEWKGKR
                     FYEPVVFWIGGETNETVTKTTQRILCGRIEENGEPGYGSIPKEDIISWKKSPFFPNLV
                     DHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIHGVWFDEEPPYSIYGEGLTRT
                     NKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMTIYDAEHYTDEQKEQIIASYP
                     EHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHFYVIGG"
BASE COUNT         6498 a         5736 c         5941 g         6166 t
ORIGIN      
        1 aaacaactgc cgctacgctt gctgatcccg cgcaacaaaa cgccatgctt tgctcgcaga
       61 tggttggcaa ccgacgacag tcctgctaaa acgttcgttt gatatcattt ttcctaaaat
      121 tgaatggcag agaatcatga gtgacagcca gacgctggtg gtaaaactcg gcaccagtgt
      181 gctaacaggc ggatcgcgcc gtctgaaccg tgcccatatc gttgaacttg ttcgccagtg
      241 cgcgcagtta catgccgccg ggcatcggat tgttattgtg acgtcgggcg cgatcgccgc
      301 cggacgtgag cacctgggtt acccggaact gccagcgact atcgcctcga aacaactgct
      361 ggcggcggta gggcagagtc gactgattca actgtgggaa cagctgtttt cgatttatgg
      421 cattcacgtc gggcaaatgc tgctgacccg tgctgatatg gaagaccgtg aacgcttcct
      481 gaacgcccgc gacaccctgc gtgcgttgct cgataacaat atcgttccgg taatcaatga
      541 gaacgatgct gtcgctacgg cagagattaa ggtcggcgat aacgataacc tttctgcgct
      601 ggcggcgatt ctggcgggtg ccgataaact gttgctgctg accgatcaaa aaggtttgta
      661 taccgctgat ccgcgcagaa atccgcaggc agaactgatt aaagatgttt acggcattga
      721 tgacgcactg cgcgcgattg ccggtgacag cgtttcaggc ctcggaactg gcggcatgag
      781 tactaaattg caggccgctg acgtggcttg ccgtgcgggt atcgacacca ttattgccgc
      841 gggcagcaag ccgggcgtta ttggtgatgt gatggaaggc atttccgtcg gtacgctgtt
      901 ccatgcccag gcgactccgc ttgaaaaccg taaacgctgg attttcggtg cgccgccggc
      961 gggtgaaatc acggtagatg aaggggcaac tgccgccatt ctggaacgcg gcagctccct
     1021 gttgccgaaa ggcattaaaa gcgtgactgg caatttctcg cgtggtgaag tcatccgcat
     1081 ttgcaacctc gaaggtcgcg atatcgccca cggtgtcagt cgttacaaca gcgatgcatt
     1141 acgccgtatt gccggacacc actcgcaaga aattgatgca atactgggat atgaatacgg
     1201 cccggttgcc gttcaccgtg atgacatgat tacccgttaa ggagctggcc gatgctggaa
     1261 caaatgggca ttgccgcgaa gcaagcctcg tataaattag cgcaactctc cagccgcgaa
     1321 aaaaatcgcg tgctggaaaa aatcgccgat gaactggaag cacaaagcga aatcatcctc
     1381 aacgctaacg cccaggatgt tgctgacgcg cgagccaatg gccttagcga agcgatgctt
     1441 gaccgtctgg cactgacgcc cgcacggctg aaaggcattg ccgacgatgt acgtcaggtg
     1501 tgcaacctcg ccgatccggt ggggcaggta atcgatggcg gcgtactgga cagcggcctg
     1561 cgtcttgagc gtcgtcgcgt accgctgggg gttattggcg tgatttatga agcgcgcccg
     1621 aacgtgacgg ttgatgtcgc ttcgctgtgc ctgaaaaccg gtaatgcggt gatcctgcgt
     1681 ggtggtaaag aaacctgtcg cactaacgcg gcaacggtgg cggtgattca ggacgccctg
     1741 aaatcctgtg gcttaccggc gggtgccgtg caggcgattg ataatcctga ccgtgcgctg
     1801 gtcagtgaaa tgctgcgtat ggataaatac atcgacatgc tgatcccgcg tggtggcgct
     1861 ggtttgcata aactgtgccg tgaacagtcg acaatcccgg tgatcacagg tggtataggc
     1921 gtatgccata tttacgttga tgaaagtgca gagatcgctg aagcattaaa agtgatcgtc
     1981 aacgcgaaaa ctcagcgtcc gagcacatgt aatacggttg aaacgttgct ggtgaataaa
     2041 aacatcgccg atagcttcct gcccgcatta agcaaacaaa tggcggaaag cggcgtgaca
     2101 ttacacgcag atgcagctgc gctgacacag ttgcaggcag gccctgcgaa ggtggtggcg
     2161 gttaaagccg aagagtatga cgatgagttt ctgtcattag atttgaacgt caaaatcgtc
     2221 agcgatcttg acgatgccat cgcccatatt cgtgaacacg gcacacaaca ctccgatgcg
     2281 atcctgaccc gcgatatgcg caacgcccag cgttttgtta acgaagtgga ttcgtccgct
     2341 gtttacgtta acgcctctac gcgttttacc gacggcggcc agtttggtct gggtgcggaa
     2401 gtggcggtaa gcacacaaaa actccacgcg cgtggcccaa tggggctgga agcactgacc
     2461 acttacaagt ggatcggcat tggtgattac accattcgtg cgtaaataaa accgggtgat
     2521 gcaaaagtag ccatttgatt cacaaggcca ttgacgcatc gcccggttag ttttaacctt
     2581 gtccaccgtg attcacgttc gtgaacatgt cctttcaggg ccgatatagc tcagttggta
     2641 gagcagcgca ttcgtaatgc gaaggtcgta ggttcgactc ctattatcgg caccatttaa
     2701 atcaataagt tacctcgcat ttaagtaaac cacgttctcc tcttgtgccg tatttgtgcc
     2761 attgcgactt ataatcgcat cgattttgct tgcgtgctcg gtgagatgcc cggctgaaag
     2821 gtgggcgtat ctttgaacca tttcgagagt ttcccatcct cccatctctt taagtgcaag
     2881 aagagagaca ccggactgaa ccagccagct tgcccaggta tgcctcagat catggaagcg
     2941 gaagttgcta atgcctgccc gctttaacgc tcccttccat gccttgttgc tgtcggttct
     3001 catcttcctt accgctgctg tttttgttcc gtcgcttcgg taggcaggtt tggtgtggac
     3061 aaatacccat ctcttatgga gcccctgctg ttttcttaat atctggcatg cggtttcgtt
     3121 aagaggaact ccgatcgcat tgccagcttt tgtttcatca gggtgcatcc atgccatttt
     3181 cttatccaga tcgacctgtg accactcaag gtctgtaacg ttggaacggc gaaggcctgt
     3241 cgtgattgca aacatgacca cagggaagaa gtgaggagca atttctgcaa acaggcgctt
     3301 cgattcctcc tctgtaagcc atctgatgcg tccattctta acgcgtggtg ttgatatttt
     3361 gggtgccctg tcaagccatc cccattcaac agccatattg agaatggcgc gaagtattgc
     3421 cagatgcctc gtcttcgttc ctttgcttgc aagctttggt ttatactccg gcaccggatt
     3481 gccaagtcgc aaacacctgt cccggctcat ctcccagttc aggcgatggc ggcggttttc
     3541 catcccgtct accgcctcca ttattttttc tgttgttatg tcagagagaa tggcttctct
     3601 gaagtgcaac atccagaacg atataatgct cttgtcatca tcaatggact tcttatccga
     3661 tttctcacgc agccaccgta tgcaggcttc cttgaatagc tttttcggtg attccccgag
     3721 attttttact ctccacgctt ctgctttcag acgatcgtga agttcttgcg cttgcctttt
     3781 gtccgatgtt tcaagagagc gtctaactct tgatccatct ggcgcgacga aatcgcagtg
     3841 ccacgtgcca ccgcgtagtt tgattgacat gctttaacct cctgcacatc aaccgcattc
     3901 accgcgctat tgtgtctcac agacttaagc gccgcaatgc agtctgactt gcaaatgcga
     3961 tatgggcttt taggtttatc tggatttatc tttgcggcct gaagtcgtcc acttcgtatc
     4021 cactgcgtga tagtgccttt gtctaccttc agatacgacg ctgcctcttc acgagtgaag
     4081 atttcttctt ccacctggaa tctccattta ttggattggt attattgcgg taggtctgga
     4141 tatcattgag caatgaacag gcctcatcga gtgtgaggcg ttagtccttg cgtagctcgc
     4201 tgattcttct gtaagtctct ggtgctttgt tcccgtacgt cttcatttca gacttcaaca
     4261 gagcaacgag tgaatcccat tcgttgagga ttcctttgaa tgccggaacg cgctttgcaa
     4321 ccttgtcgaa tgaatctctg atttctggaa tctgctcaac aagtgcaacg catcgccgga
     4381 aatcggctgc gtcatgtgga gcaccgaagc tatgaccata gatattcttt ttcagtccac
     4441 atgcgattga ggcaagagtt gcgctactga tgccgacatc gtcagtcgat tgccatttca
     4501 aaaccttcat agccaaatct gacatttctt gtctccaata aaaaaccgcc atcaggcggc
     4561 ttggtgttct ttcaattctt caattcgaat attggttaca ttgttttcat atatgaataa
     4621 ataaattagc ttttttcgtt gccttcgcgt tctttattaa ttttgacaaa ctcgttttta
     4681 ccacgctctc caaatgcgtc tttagagtcg ttgtatccgc aatcgcagca cacataatca
     4741 ccagaccatc cacgcattgt tttttctttt gcaatatttc cagaaccgca ttttggacaa
     4801 gacatatcac tacctccaaa gcatgagtga gatgacaacg taacattgat tggagattaa
     4861 caatagattg ctgatgtaaa agatatgtat aagcttcgct ttcaaagtgg aggctctggt
     4921 agcggcatcc agtgggttac attgctaatc agaccatatt cattagtttg aggatggttg
     4981 ccgttatcgt ctccgtattt aagactctcc ataaaaccat aatgcttatt cccattaacg
     5041 ctcacaaagc cgtaataagc tggtataacg ccgatctcac acgtaatcag caaaggaaaa
     5101 ctagttctcc aatttagctc gccgattaca ggcattctct cactacagct tatccaacca
     5161 tccggagtta ccggaacttg cggaatggct gtctgctctc gaacgtcatt aggcgcgata
     5221 ggttctgctg ccaactgact ggcatatttg ttaatggtaa cgataagctc ttgctcagcc
     5281 tcatccagac aatcaccgat acctcgcctg tcaccgtcaa aatcatcgaa atcggcacga
     5341 atcttggcaa ccttctggat tgcggacaac acctcactag gaattaccgg atagttggtt
     5401 gacgtttccg cgatttcacg aaaattattg gttgacgaat tcttgttttc ccgaaagttt
     5461 ccggactgaa gcatggctgc gcgataggcg ttccagccga cagcttttcc gtgttcaaac
     5521 gcgctgtcaa agtcatcatc catttccatc gcatcaggca cagataccgt ctctggaggg
     5581 gcggcgtaaa gcggagttac ggtaatttcg tactcgttta tatcctcgtc gctgatattt
     5641 gcgaaatagc gaaggatgcc tttctctcca cgactacgag cgtcagagtc ctgaatgata
     5701 tacgccacag gctctgcttc cagcgatgcc agcgcaattc gtgccagttc catttgttcg
     5761 ccacgagtaa gcccgttttc aagcggattt ttaatgaaca attcaatacg ttctttggta
     5821 atagtggtca tagctatttc accttaatct caatatttcg cagctttagc tctactggca
     5881 ggtctgactt tcctgttaac gctaatgcga gattttctgg agtaatgaga gcagttattg
     5941 ttttccccat cgccagacga ataatcagtc gtatctcgcg atcgtcacat gctcctggtc
     6001 gaacaattga tatttgtccg tccatctcac tctcctttga tgcgaatgcc agcggcgcgg
     6061 attgcagcga tgacctcaga aactttgtat gccattaccg tttggtaatc atcgtgaaaa
     6121 tctgttcgat gaagcatgct gctacgttcc gggagcagta ttccccgctc ccccagttct
     6181 gctatgcgat tctctgcgga ttccagcgcc gcaaccaatt cgtctacagt tccggcagct
     6241 tgcagtgcgt aatcggtaat agccatctca tgatcaattt cagtaccgtt ctcattcgtt
     6301 gaggtgatag caaaataatc agagtcgatt tcgttatcag ctaagtggcg tagcgtatcg
     6361 gcaacaagcc ggccgttttc gattagcagc ttccctaccg ttagcgcaat atcctcgttc
     6421 tcctggtcgc gggatttgat gtattgctga ttccttaccc gttcatccag tagtgccagc
     6481 acggtttctg gtccggccag aaatttgaag gcgttgagcg catcaatatc cacaccgtaa
     6541 tctttaagtt cctgttcact taacaaatca tcagctggca acattaacag gcgttccatt
     6601 gctggaattg cacgttccgc cgcctcacgc agtgcctgat agttaatttc gctcactggc
     6661 cgcctccttt gcgaagctgg gcggcgaact cgttagccat cgctgaatat gggtgctcat
     6721 gaccgccaac aaccttcatg ctggcagcaa acatctccac gccctgcgcc cgcacttcat
     6781 ccaggaaagt gtcggtggct ggggtttctg gaatggcgtc tggaataacc tctgagtaga
     6841 cgcgatccat tgccgactcc catccgtagt aacaagccga atagccgtcg cgctggtagc
     6901 cgtggtcttc aactccacat cccatgccct ggtcatggta ttcaggctga ttgtcgggat
     6961 taatgcagta ttcaatgacc gacttcatcc ccgcattctc cgccgccagc gccgaaaact
     7021 tctcgtgtgc caacttaaca gccgcatcag cctgcttaat tgactcaatc gctttctggt
     7081 ggtcttcgta cagagccgaa atcttggcct ccgcttcagc aaatttacgc accagatatt
     7141 cagcgtttgt ttcgttaacc tttaaatcac atgggatgca tttacctttc agaaaaccat
     7201 ccatctcaat tagtgacatt tgtttcattt cttcccactc cgccacatag cattcagata
     7261 tttgttgtca ttaacagaac cgaaactctt tctcttaagt aattcctctc tcgatggcat
     7321 tggctttacg cgttggcgaa taatcatttc tgccggaaga atgccgggat tgtatgcaag
     7381 tcctctcatg gtaaattcct cagtcattac tgatagcgcc atagcgtgag cggtaattac
     7441 gcaggcgcgg gtcaatttca gggaagtggg tatatgtggc tttgcggaat ggtcggattg
     7501 atgtctggta aattcgctcg agttcttctt tctctgcaag ccatatacag tggcgaaatt
     7561 ccttttcctc tttcgtttcc tgcggtagcg acattatccg atcgtagttt tttctgaatt
     7621 tatccagcac ctccgatacg gaattgccgg aacagcggcg tgggtcatcc gcaccataca
     7681 gaggcgctgg cataatggaa tccttatgtt gctactttag aagggaattg aatcgtcgta
     7741 ttcaggatga ttttgatgat tgctactttg ctgctgttgg ctgtttcctg aagttgcaaa
     7801 tccaatcttt gcattcagta attcaagagt gattgattgt ccattttgcc cctgataaac
     7861 atcaaccctg atgttttctc cggtaatttc cacaatgcca ccttcaacaa gaacactacg
     7921 gtagtaatcc gcttgcgctc ccggcttggc aaatacaacg gcgctgtagt ttgtccattc
     7981 tttctttttt gtctggcgat cgtaatactg aacgccagca cggatgttga atccgatatt
     8041 ttccccggcc tgaaactctc ttgcgggctt gtttagtctt acagtaatcg aatgtgccat
     8101 taagcagccg ctccttctaa ttcgtctcgt ctgatgttgt aaatgtcctg cgctttgtgc
     8161 tgctccggtg tgccttcgag catcttccac gctttggcga acgcctgttt aagctcttcc
     8221 acggtgtttt tctgcaatgc tgcgtcagtg aatgctttta gaacctgttc aggtgtaggt
     8281 gatggttttg attgctttgc tgctgcgttc tgctgatgtt tatgctcgtc ggtatctgca
     8341 tctttcgcat catcaatgcc gaacaaacca ttgaggcaat acttgcgtgc ataagagctt
     8401 gtggctcccg taacttgtgc agaatccatt cctttcttgc tttcttcctc tcgtgcaaga
     8461 gcggttgccg tatgactgtt ttcgccatcg gtaatagttg ccgtggcttt cacgtaatac
     8521 cgatcaccaa tcaacacaac ttcatcgctg attgataaaa acagaccatt cagtaacggc
     8581 ttaacgcctt caagaatgtc ttcgcagctt ctgtatttat atttaccgaa tgagttgtac
     8641 tggttctttg gcgcgttcaa gttctcctga atagctgcca gtcttgcgta aaattctttg
     8701 ctcatatgtt tgttctcaga atgggcatgg cccaaggaaa taacgctgat ttaatacttc
     8761 gactcgggac aaattaaggc atacccgcat tccttcgcgg tcgccattat ggcgatacca
     8821 gagagctttc tgcgtgtaca tgcgtctctg taacttgctc tccttcactg tggttgcaag
     8881 tgacatgaat atctccttcg ttaccgatta attctttcat ctgacgaatg aattcttcgt
     8941 ctgaccagtt atctgtaaaa ctcatttcct gcgataccac ggaatattga tagctgattt
     9001 catcgcttta tttgcttcaa gccacatttt tgaatcacca ataaatctgg ctattactgc
     9061 tttgttttgt gctgcacgaa gcatctggtg attgatggct atttcattgc gcataacgcc
     9121 tccggttgtt tctttgctgc actgattaat tgtttaactc ggcgtgataa ttcagattcg
     9181 tgcgggtaga aagcggacat gacgccgcta cccgcgagct gaaagtgcat catggataac
     9241 tccttctgtt tgattgcata acgaaaacgc ctcgagtgaa gcgttattgg tatgcatata
     9301 aaaaagccct catactggag ggcaaagaag atttcaaata atcagaacaa gtcggctcct
     9361 gtttagttac gagcgacatt gctccgtgta ttcactcgtt ggaatgaata cacagtgctt
     9421 actcgtacta ataaaatacc caattttctg tttcttggtt gtgcccaaag ttatattcaa
     9481 tatctggtgt tgatgtatca atattcttca tcccatcaac aagagttgat acaacagcca
     9541 aatcctgttt gattctcatt aaatggtatt tcttccggcg caataaactt tcaatggcaa
     9601 gtttcttcgt tgggaatgca aaagatcttt ctgcattttt tgctactttc ttaattgcat
     9661 atctatttct cttttgtttc cattcctgta accactgatt tggtgctggt ttaaaattaa
     9721 caatccaatg cgcaggaacc aaccatgcat aatgctctgt ctgatgaaaa gctatatatt
     9781 gaagtgcgaa tattttgatt ccatcttctt caactgtcgc ctggaatctc cagaaaacag
     9841 gcattccatc atgttcagtt tctgattcag gaaaaggtac gctccatgat tttgtcatat
     9901 ctcacctcaa ataagtggct tgctgcctaa tttcattttc tggcgaccaa cacaagtcac
     9961 acccatttca ctgcgtggct tgctgtacca tgtgcgctga ttcttgcgct caatacgttg
    10021 caggttgctt tcaatctgtt cgtggtattc agccagcacc gtaaggtcta tcggattcag
    10081 tgcgctttct actcgtgatt tcggtttgcg attcagcgag agaatagggc ggttaactgg
    10141 ttttgcgctt accccaacca acaggggatt tgctgctttc cattgagcct gtttctctgc
    10201 gcgacgttcg cggcggcgtg tttgtgcatc catcttgatt ctcctgtcag ttagctttgg
    10261 tggtgtggta ggtgggagac ccatttcgac ctgcttcggc cgacttcaat tcggcaatag
    10321 ttccgcaggc ctcgccgctt tacgtgcgac atattcccat ccatgaaccc ttcaccacac
    10381 cccaaagcct tctgctttga atgctgccct tcttcagggc ttaattttta agagcctcac
    10441 cttcatggtg gtcagtgcgt cctgctgatg gcttaaaatt acaagaaaga ttgtatgttg
    10501 taaacaagaa atattgtaaa aaggggcgta aaaacaaact ccattgtttt taaatgaaaa
    10561 atagtttgtt tttggttatc gagattgagg tggggattac tggttgcagg ttccgactac
    10621 atcaccaaca aatgatttgg ttgatgtaag ttgttgcata cctgggatat tcattacttt
    10681 ggagtaaaga gcttttttgt ctgtagtgat tgatcaagtt tcaacggtta ttcctcctcc
    10741 agactggtat tctcctacca tagtgttcga tgacaaagca gtgtatttca tctctggata
    10801 gacgccagaa actgattcat aaactgatga tttatcgcca tttattgtta cgtggaaaac
    10861 ggaatcttcc gtgctgtctt ttgtaaactc gtaacgatcg ccattcattg acccgtaccc
    10921 gtgcaggttt gtgacaatcc agcattcaga attggcgctg gtagttaaga gtattgagag
    10981 tagcgccgca atcctgatca tacgaatttt accctcgctt ccacgacaac accgataatc
    11041 ttgcagttcc cgttgatagg agtcataggc catgaagggt tcaggccttt caggtacttc
    11101 tgcccgccat ctataaccag tttcttgaat gttgcttcgt tcgcgtcagt cagtttggct
    11161 acaacaaggc ttccattcac tggctcgcgt ccagtatcta ctaacaccat atgaccttca
    11221 gggatgcttt gacctacagg tgaggtcatg gaatcacctt caaccttcag ccagaatcca
    11281 tcgcctaata agttaacgtc actgtcatac cattcatcaa tgtccttgat atcgtagggt
    11341 tcacaagctt cacaccacga accagctcta accatgctaa tcaatggata tttccctttg
    11401 ggctcaacat gcccaacaaa tctaacattc gaatcagagg tgccattgag cagccagtca
    11461 acacttacgc caagagctga cgcaagttct ggtaaaaagc gtggtcgctt agttttaccg
    11521 ttttcgagct gctctataga ctgctgggta gtccccacct tttgagcaag ttcagcctgg
    11581 ttaagtccaa gctgaattct tttgcttttt accctggaag aaatactcat aagccacctc
    11641 tgttatttac ctccaatctt cacaagaaaa actgtatttg acaaacaaga tacattgtat
    11701 gaaaatacaa gaaagtttgt tgatggaggc gatatgcaaa ctctttctga acgcctcaag
    11761 aagaggcgaa ttgcgttaaa aatgacgcaa accgaactgg caaccaaagc cggtgttaaa
    11821 cagcaatcaa ttcaactgat tgaagctgga gtaaccaagc gaccgcgctt cttgtttgag
    11881 attgctatgg cgcttaactg tgatccggtt tggttacagt acggaactaa acgcggtaaa
    11941 gccgcttaag acattcccgc tcttacacat cccagccctg aaaaagggca ttaccagaaa
    12001 caaatctcta tggttttgcg tttctttgcg aagccaactc tatctaatca ttaaggaaat
    12061 tatctatggg tactattgca actaaaagca agaaagcggc tcgcatcgag tcagccttgc
    12121 tgaacaaact ggcactgatg gggcagaaga cattcgctcg agcaatgggg gttcctgaat
    12181 atcaggtaag ccgatggaag aatggtttct tctcgcaggt aagcatgatg cttgctgttc
    12241 tggaatacgg aatcgaagac gatgaaatgg ctgaattgac taagcggctt gccgattacc
    12301 tgacaaaaga aaaagccccg aagaacggcg aattcttcga ggcctaatgt agaaagactg
    12361 gatcaatcca cgggagtaat tatgacaaaa cgtcgtaaga aataccagga aaaagaagag
    12421 attcgacacc ctgattcacc tgagggatta gtggtagccg cagcaaataa cagggcgttc
    12481 gcagagcgcc ttgttggtgt ttacagacta gccaaagcag gagtgaaaca tgggcgtcgt
    12541 taagttagct gattacaggc ctcaactgga ggtcgtggag catcgcgtgg cagaactcga
    12601 agatggctac actcggactg caaacacact gttagaagcc gccatgcttt ctggacttac
    12661 tctacatcag ttactgattg ttatggctgt gtggcgcaag acatacggtt acaacaaaaa
    12721 aatagattgg atcggaaacg aacagttcgc tgaactcact ggcatggcac caaccaaatg
    12781 ctctaccgcc aaaaacgagc ttatcagaat gggagttctc attcaggtgg ggcgtcaagt
    12841 tggtatgaat acaaacattt ccgagtggaa aacgaaagtt aacggattcg gtaaaacatt
    12901 taccaattcg gtaaaacaaa ccttcaccaa atcggtaaaa agcaatttac cgaatcagtc
    12961 aaacacaaaa gacaatatac aaaagacaat aaatacaaat acccccttac cccctaacgg
    13021 gggcggcgat gggcaggtta aacctgaacg tcgcaaggca gaacgaatcg actacgaatc
    13081 cttcctgaac gcctacaaca ccgaagtcgg tgacagactg ccacacgctg ttgcggtcaa
    13141 cgagaaacgc aaacgccgcc tgaagaaaat catcccgcaa ctgaaaacgc caaacgtgga
    13201 cggtttcaga gcgtatgtca gggcgtttgt gcatcaggcc aagccgtttt acttcggaga
    13261 caacgacacg ggctggacgg ccgattttga ttacctgctg agggaagact cgttaacggg
    13321 ggtacgggaa gggaagtttg cagacagggg gattgcatga aacaggatat cgaagcgagc
    13381 gttatcggtg gcctgctgat tggtggatta acaccaaccg ccagcgacgt tctggcaacg
    13441 ctggagccgg aagcgttttc aattccgctc taccggaaag ccttcgaggt tatccgcaag
    13501 caggcgcgaa acagaaacct aatcgacgcg ctgatggttg ccgaggcgtg cggagaggag
    13561 catttcacgt caatcctgat gaccagcaag aactgcccga gtgccgcaaa cctgaaggga
    13621 tatgctggaa tggtcgcgga taactatcac cgccgtctgg tgctggaaat catggatgaa
    13681 atgcgtgaac caatccaaag cggaaccatc gacgcatcga gtcaggcgat ggatgaactt
    13741 gtaaagcgtc tttcagccat cagaaagccc cgtgacgagg taaaacctgt acggttaggg
    13801 gaaatcatca ctgactacac tgacacgctt gacaggcgtc tgaggaacgg agaagagtca
    13861 gataccctga agaccggaat cgacgaactt gacgccatca ccggagggat gaacgcagaa
    13921 gacctggtga ttatcgccgc tcgtcctggt atgggtaaaa ccgaactggc gctgaagatt
    13981 gccgaaggcg ttgcaagccg cgttattcct ggttctgacg tccggcgcgg agtgttgatt
    14041 ttctcgatgg aaatgagcgc attgcagatt gcagagcgga gcattgccaa cgccgggagg
    14101 atgtcggtta gcgtgctgcg aaatcctgca tcgatggatg acgaaggctg ggcgcgtgtt
    14161 gctaacggca tgagtcagct tgcagatttg gatgtatggg tagtcgatgc ctcgcggtta
    14221 tcggtcgaag aaatacgctc aatcgcagaa cggcacaaac aggaaaatcc aaacctgtca
    14281 ctcatcatgg cggattatct tggcctgatt gagaagccga aagcagaccg caacgacctc
    14341 gcaattgctc acatctccgg aagcctgaag gcgatggcga aagacctgaa aacgccagtt
    14401 atctccctga gtcagctttc gcgcgatgtt gagaagcgac caaacaaacg cccgacaaac
    14461 gcagatttgc gtgattcagg aagcattgaa caggacgcag actcaatcat catgctctat
    14521 cgggaagcgg tatatgacga gaacagtagc gccgcgccat ttgctgaaat catcgtgacg
    14581 aaaaaccgtt ttggctcgct tggtacggtt taccagcggt tctgtaacgg acactttgtt
    14641 gcatgtgacc aggatgaagc cagacagatt tgcacagcat caaatgcacc tgctgcgcgt
    14701 ggcagacgat atgcacaagg ggctgacgta tgaccatcta catcactgag ataataacag
    14761 gcctgctggt aatcgcaggc ctttttattt gggggagagg gaagtgtggc tgactggcaa
    14821 attccaatca tcattcttgc cggagcttcg ctggttgctg gctttatcct gctgaaaaag
    14881 cataaagacc gtgatcaaaa agttgaagtt ctctatgggt atccagcgaa cagcacaaca
    14941 tggctgacca tttaccacta ccgaaaatca ggacgctggg tattcgaatg ggatgatctg
    15001 ttcgctgaaa agcgaccaaa gtcatgggga gacatcagcg aatgcatgat gtttgaagaa
    15061 agaaaatccg gcgcaacccg agaagagttt aacgaagcgt gggcgcgatt aagtgagaga
    15121 gggtatttgt gagcaagtac gaaaaattag atcaaaacat tctttcaatg ctgagtgaaa
    15181 gaccaacacc tgtttttgat atctggctta aatggcggag caatggaatg tatatcgaaa
    15241 ccatcgatcg ccgtatgcaa tacctgagaa agaaagggct tgttgcaaat gtgcgtggga
    15301 agggttgggt gaaaattaac ctgtcataac ggggattgat atggacgata gcttagaaat
    15361 atttgaagag tggtgcgctg atgaacttgg tgtctccgct tacttcattc gacaaatgcg
    15421 aagcaagaat attttgggcg tgattgagta taaacgagtt gaaataaaca agagatacag
    15481 ggcttggatg gccgcagttc gcgccgccgg agtcaaagtg aaggagtgag tatgagcgca
    15541 tacgaagaaa tcatgttagc cctgcgattc tttttcggtg tggaagaaga tgaaaacgta
    15601 aatgagatta tcggacaaga ccatgacccg atagggacta ttgcagctgc acttgacgat
    15661 tacaggagcg taaatggtga ggaaactaac gtttgaacta agaagcccca tccatcagca
    15721 gaacgccatt caagctatcc agcaaatcct tccagaccca accaaaccaa tcgtagtaac
    15781 cattcaggaa cgcaaccgca gcttagacca gaatcgaaag ctttgggctt gccttggtga
    15841 cgtttcgcgt caggttgaat ggcatggtcg ctggctggat gcagaaagct ggaagtgtgt
    15901 gtttaccgca gcattaaagc agcaggacgt tgttcctaac cttgccggga atggatttgt
    15961 ggtaataggc cggtcaacca gcaggatgcg tgtaggcgaa tttgcggagc tattagagct
    16021 tatacaggca ttcggtacag aacgtggcgt taaatggtca gacgaagcgc gactggctct
    16081 ggagtggaaa gcgagatggg gagataaaac tgaatgaaca aataccgact tatttacgca
    16141 gatccgcctt ggcaatatcg cgacaaagcc aacgatggca atcgcggtgc tggacataaa
    16201 tacgatgtta tgaatgttca ggacatttgc cgactgccag tatgggattt agcggatcca
    16261 gaatcttgct tgttagcgat gtggtgggtg ccgacacagc cagccgaagc gctaaaggta
    16321 attgaggcgt ggggattcag gttgatgact atgaaaggct ttacttggca caaaaccaat
    16381 aagcacaaag gcaacagtgc gatcggaatg gggcatatga ccagggcaaa tagcgaggat
    16441 tgcttgtttg ccgttcgagg gcggttacct gagagaatgg acgcttcaat atgccagcac
    16501 tttaccgcac cgagaatgga gcacagtgca aaaccaccga tcgtaagaga catgttagct
    16561 aagttgcttg gcgacgtgcc gcgctgtgag ttatttagcc gcgacaaagt gaccgggtgg
    16621 gatatgtggg gaaatcagtg cgactccgat tttgaacttg ctcccggcat ggcgattaaa
    16681 ccatgcaaaa tggtgatcgc atgaagcact gttatcgatg tggagagcga aaggaagacg
    16741 atcgctttcg accaggacag ccttactgga atcgatggtg tctccggtgt gaaagaacac
    16801 caacaggggt gttaccacta ccgcaggaaa aggaggacgt gtggcgagac agcgacgaag
    16861 tatcaccgac ataatctgtg aaactgcaaa taccttccaa cgaaacgctc cagaaataaa
    16921 cgcaagccaa tcccaaaaga atctgacgta aaaaccttca actacacggc tcacctgtgg
    16981 gatatccggt ggctaagaca tcgtgcgagg aaatgacaat gcttttaatt caacctggat
    17041 ttggacttag catcaaaaaa ggccacatgt ttggcgagaa agagtcacaa cgaaaaatga
    17101 tgtctatccg gttgccattt atcagtattt attggctaaa cagggaggca acaaattatt
    17161 ggtatacatg cgccagagca gcatttaacg accctgactg gtttgtggaa aaccaccacg
    17221 cagttcgtca ggcaaagaga aaggccaaca tgacatacat gaaggcgtat cgaaaagcat
    17281 ggaaagaaca ccgcgatcga taccagcaag atatggaaaa gcttgaatca gaaaacatgg
    17341 aattaagacg aaagcttggt gaagcaaaac gagacattga tgcttacaag cgacttttta
    17401 atggtgaaag ccatgcttag tccatcccaa tctcttcaat accagaaaga aagcgtcgag
    17461 cgggctttaa cgtgcgctaa ctgcggtcag aagctgcatg tgctggaagt tcacgtgtgt
    17521 gagcactgct gcgcagaact gatgagcgat ccgaatagtt caatgtacga ggaagaagac
    17581 gatggccaaa ccagcgcgaa gacgatgtaa aaacgaagaa tgtcgggaat ggtttcaccc
    17641 tgcattcgct aatcagtggt ggtgctctcc agagtgtgga accaagatag cactcgaacg
    17701 acgaagtaaa gaacgcgaaa aagcggaaaa agcagcagag aagaaacgac gacgagagga
    17761 gcagaaacag aaagataaac ttaagattcg aaaactcgcc ttaaagcccc gcagttactg
    17821 gattaaacaa gcccaacaag ccgtaaacgc cttcatcaga gaaagagacc gcgacttacc
    17881 atgtatctcg tgcggaacgc tcacgtctgc tcagtgggat gccggacatt accggacaac
    17941 tgctgcggca cctcaactcc gatttgatga acgcaatatt cacaagcaat gcgtggtgtg
    18001 caaccagcac aaaagcggaa atctcgttcc gtatcgcgtc gaactgatta accgcatcgg
    18061 gcaggaagca gtagacgaaa tcgaatcaaa ccataaccgc catcgctgga ctgtcgtaga
    18121 gtgcaaggcg atcaaggcag agtaccaaca gaaactcaaa gacctgcgaa atagcagaag
    18181 tgaggccgca tgacgttctc agtaaaaacc attccagaca tgctcgttga agcatacgga
    18241 aaccagacag aagtagcacg cagactgaaa tgtagtcgcg gtacggtcag aaaatacgtt
    18301 gatgataaag acgggaaaat gcacgccatc gtcaacgacg ttctcatggt tcatcgcgga
    18361 tggagtgaaa gagatgcgct attacgaaaa aattgatggc agcaaatacc gaaatatttg
    18421 ggtagttggc gatctgcacg gatgctacac gaacctgatg aaaaaactgg agacgatagg
    18481 attcgacacc aaaaaagacc tgcttatctc ggttggcgat ttggtcgatc gcggtacaga
    18541 gaacgtcgaa tgcctggaat taatcacatt cccctggttc agagctgtac gtggaaacca
    18601 tgagcaaatg atgattgatg gcttatcaga gcgtggaaac gtcaatcact ggctacttaa
    18661 tggcggcggc tggttcttta atatcgatta cgacaaagaa attctggcta aagctctttc
    18721 ccataaagca gatgaacttc cgttaatcat cgaactggtg agcaaagata aaaaatatgt
    18781 catctgccac gccgattatc cttgtgacga atacgagttt ggaaagccag ttgatcatca
    18841 gcaggtaatc tggaaccgcg aacgaatcag caactcacaa gacgggatcg tgaaagaaat
    18901 caaaggcgcg gacacgttca tctttggtca tacgccagca gtgaaaccac tcaaatttgc
    18961 caaccagatg tatatcgata ccggcgcagt gttctgcgga aacctcacat tgattcaggt
    19021 acagggagaa ggcgcatgag actcgaaagc gtagctaaat ttcattcgcc aaaaagcccg
    19081 atgatgagtg actcaccacg ggccacggct tctgactctc tttccggtac tgatgtgatg
    19141 gctgctatgg ggatggcgca atcacaagcc ggattcggaa tggctgcatt ctgcggtaag
    19201 catgaactca gccagaacga caaacaaaag gctatcaact atctgatgca atttgcacac
    19261 aaggtatcgg ggaaataccg tggtgtggca aagctcgaag gaaatactaa ggcaaaggta
    19321 ctgcaagtgc tcgcaacatt cgcttatgcg gattattgcc gtagtgccgc gacgcctggc
    19381 gcaagatgca gagattgcca cggtacaggt cgtgcggttg atatagccaa aacagagcag
    19441 tgggggagag ttgtcgagaa agagtgcgga agatgcaaag gcgtcggcta ttcaaggatg
    19501 ccagcaagcg cagcatatcg cgctgtaacg atgctaatcc caaaccttac ccaacccacc
    19561 tggtcacgca ctgttaagcc gctgtatgac gctctggtgg tgcaatgcca caaggaagag
    19621 tcaatcgcag acaacatttt gaatgcggtc acacgttagc agcatgattg ccacggatgg
    19681 caacatatta acggcatgat attgacttat tgaataaaat tgggtaaatt tgactcaacg
    19741 atggataaat gcactcgtta aataaagccc tgagttaata gctcggggct ttttgcgttt
    19801 taagcacggc ctttctgaaa gcacatcaag ccaaatacca gacagacaaa aataatcacc
    19861 ttatccgctt ggctacggtg cggtgtgctt tgcataaaag aaaaccagcg caatggctgg
    19921 cttcgtgaaa gcgggtggca agaggctgcg ctaacaacct catgccgttt tgcccgtgca
    19981 tatcggtcac gaacaaatct gattactaaa cacagtagcc tggatttgtt ctatcagtaa
    20041 tcgaccttat tcctaattaa atagagcaaa tccccttatt gggggtaaga catgaagatg
    20101 ccagaaaaac atgacctgtt agccgccatt ctcgcggcaa aggaacaagg catcggggca
    20161 atccttgcgt ttgcaatggc gtaccttcgc ggcagatata atggcggtgc gtttacaaaa
    20221 acagtaatcg acgcaacgat gtgcgccatt atcgcctggt tcattcgtga ccttctcgac
    20281 ttcgccggac taagtagcaa tctcgcttat ataacgagcg tgttcatcgg ctacatcggt
    20341 actgactcga ttggttcgct tatcaaacgc ttcgctgcta aaaaagccgg agtagaagat
    20401 ggtggaaatc aataatcaac gtaaggcgtt cctcgatatg ctggcgtggt cagagggaac
    20461 agataacgga cgacagaaaa ccagaaatca tggttatgac gtcattgtag gcggagagct
    20521 atttactgat tactccgatc accctcgcaa acttgtcacg ctaaacccaa aactcaaatc
    20581 aacagcagcc gggcgctatc agcttctttc ccgttggtgg gatgcctacc gtaagcagct
    20641 tggcctgaaa gacttctctc cgaaaagcca ggacgctgtg gcattgcaac agattaaaga
    20701 gcgtggcgct ttgccgatga ttgatcgcgg tgatatccgt caggcaatcg accgttgcag
    20761 caatatctgg gcttcgttgc ccggggctgg ttatggccag ttcgagcata aggctgacag
    20821 cctgattgca aaattcaaag aagcaggcgg aacggtcaga gagattgagg tatgagcaga
    20881 gtaaccgcga ttatctccgc tctggttatc tgcatcatcg tctgcctgtc atgggctgtt
    20941 aatcattacc gtgataacgc catcgcctac aaagagcagc gcgacaaagc cgcatccact
    21001 atcgctgata tgcagaagcg tcaacgtgac gtagcagaac tcgacgccag atatacaaag
    21061 gagcttgctg atgctaacgc gactatcgaa agcctccgtg ctgatgtttc tgctggtcgt
    21121 aagcgcctgc aagtcgccgc cacctgtgca aagtcaacga ccggagccag cagcatgggc
    21181 gatggagaaa gcccaagact tacagcagat gctgaactca attattaccg tctccgaagc
    21241 ggaatcgaca ggataaccgc gcaggttaac tacctgcagg aatacatcag gactcagtgc
    21301 ctgaaataat ttttttgcaa atcacaaagt ccatttaatg agcctcgcga tgcggggctt
    21361 ttttacatct gaatttcaca gcgcatctca cgcgcatatt acatcacccg agcctttcag
    21421 aaagttgagc ctgagaactg ccgtatatgg tggcgaccat ctcgggacgg cttttctgtg
    21481 agacaggctc actttctaaa aggtaaagac gcaatgaact acccaaccgt tgttaacgat
    21541 atagatttca gagacctaat ttttgtagca aacaacgatc cggttacaga ttcttttatg
    21601 gtggcaaaag catttggaaa gctgccgaag aacgtagttc gtgacattga gcgaaccata
    21661 gaagcttgcc ctcctgagtt tgatacaaag ctcaactttg agctttgcta taaaaacaat
    21721 gagttacaga atggtaagcc gcaaaaattc taccgtctcc gcaaggatgg gttgatgctt
    21781 ttggttatgt cctacaccaa aaaagaagca atgcgtatca aaattgctta catcaacgca
    21841 tttaactgga tgtacgccat gcttcaggtt ggtcatcgtc aatttgaaga agagagaaat
    21901 gccgtaatgc tggagtacat gaaagagaag gatgttgcca gcatgtcagg tcgcctgcta
    21961 aatcgctggg gcaaaattaa gaagccacag ctgatggcta gaattgaacg ccttgaacag
    22021 cacgggcaaa ccgtaatccc cggactcatc aattaacggc agcaccgcga aacaacccaa
    22081 gccagtaagt ggggaaataa cactggcagc cactgaaaga tgaacctcca gccttatggc
    22141 aaaaaagatt ctttgtggtg gcggactgat ggaaagacat cggatagaat caagcagtgg
    22201 ctagggtagc tcccgaaaag cggactcgtc accgcctgcc actgaatcta tggcgaacaa
    22261 ctagacgagg ttgtgatgga tgataacgga aaccaatggg tcaatgttga acaccggcta
    22321 ccagaaagca aagaagggat gtggtctaaa gaggttatag ctcttactga tactggtgac
    22381 gtgttcaaac tatcatgtat gggctcttac tggcagagaa ccaaggcatt catcgattca
    22441 ggagcaagca aggttacaca ctggatgccg cttaattacc cagatgatta aaacggatga
    22501 aaaatgaaag gtcgctcagg cggccttttt tattgccatc acaaaagcca ttccttactg
    22561 agtggctttg ataatggctt ataccctaca cgggataact taactgatat cccttttaac
    22621 ggataaatgg agccaacaat ggcagagatt attcccatga ctgaagaaca gaaattccag
    22681 ttagagattt acaaactggt catgaaccag aacgcagccg cagaagaagc atttcaattc
    22741 attggcactg acgaactgaa gcttgagcta ttcaaaattc acttccagtc aggtggcgct
    22801 aattcagata tcacgatccg cacatttgaa gcggtgcgta aatcgaagga agcgttagac
    22861 ctgttcacca ccggagcatg atgctcaacc tgaaataacg attaagtgag atgaatatgg
    22921 cagcaccaaa gggcaaccga ttctgggagg cccgcagtag tcatgggcga aaccctaaat
    22981 tcgaatcgcc tgaggcgctg tgggctgctt gttgtgaata cttcgagtgg gctgatgata
    23041 acccgctatg ggagggtaag gtattttcat atcagggaga aataattaag gctaatgtcc
    23101 ctaagatgcg agccatgact atttcaggat tgtgtacctt ccttgatatc accaggcaaa
    23161 catggggaac cttccggtca atggaaggtt tttctgacgt cacatcacga gcggaagaca
    23221 tcatctacga ccagaaattc tctggcgcag ccgctgacct tctcaacgct aacatcatcg
    23281 cccgtgattt gggcctcaaa gagcagtcgc aagttgaaga cgtgacacct gataagggag
    23341 atcgcgataa gcggcgctct cgtatcaagg agctattcaa ccgtggaact ggacgcgatt
    23401 cttgataact tgagcgacga agagcaaatc gaattgctcg agctactcga agaagaagag
    23461 aactaccgta acacacacct gctatatgaa tttacgccat acagcaaaca gcgtgagttc
    23521 atcgacgccg ggcatgacta tccagagcgc tgttttatgg ctggtaacca gcttggtaag
    23581 tcatttactg gtgctgctga agtcgcgttt caccttaccg ggcgttatcc gggcacaaaa
    23641 ggctatcctg ctgatggtaa atatggcggt gagtggaaag gtaagcgttt ttatgagcct
    23701 gttgtctttt ggattggtgg cgagacaaac gagactgtaa ccaaaacgac tcaacgcatc
    23761 ctgtgtggtc gtatcgaaga gaatggtgag ccaggctacg gttccatacc taaagaagac
    23821 atcattagct ggaagaagtc tcctttcttt ccgaaccttg ttgatcacct tctggttaag
    23881 catcacacgg ctgatggcgt tgaagatggc atttcaatct gctacttcaa gccatactcg
    23941 caaggtcgtg ctcgctggca gggtgacaca atccacggcg tgtggtttga cgaagagcca
    24001 ccatacagca tttatggtga aggccttacc cgtaccaaca aatacgggca attctcaatt
    24061 ctgacgttta ccccgctgat ggggatgtct gacgttgtta ccaagttcct gaagaatcca
    24121 agcaagtcgc agaaagtggt caacatgacc atctacgacg ctgagcacta cacagacgaa
    24181 cagaaagagc aaatcatcgc atcttatcct gagcatgaga gagaggcgcg tgctcgcggt
    24241 attcctacga tgggtagtgg tcgaatcttc cagataccgg aagagacgat taagtgtcag
    24301 ccgttcgagt gtcctgatca cttctacgta attggcggga t
//
LOCUS       DAJTUP010000058        24186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTUP010000058 DAJTUP010000000
VERSION     DAJTUP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.058"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(25..597)
                     /locus_tag="OO468_002394"
     CDS             complement(25..597)
                     /locus_tag="OO468_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR6259478.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            815..1675
                     /gene="tesB"
                     /locus_tag="OO468_002395"
     CDS             815..1675
                     /gene="tesB"
                     /locus_tag="OO468_002395"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR6259479.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO468_002396"
     CDS             complement(1724..3010)
                     /gene="amtB"
                     /locus_tag="OO468_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR6259480.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO468_002397"
     CDS             complement(3040..3378)
                     /gene="glnK"
                     /locus_tag="OO468_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR6259481.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3559..5340)
                     /locus_tag="OO468_002398"
     CDS             complement(3559..5340)
                     /locus_tag="OO468_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6259482.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5333..7105)
                     /locus_tag="OO468_002399"
     CDS             complement(5333..7105)
                     /locus_tag="OO468_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR6259483.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO468_002400"
     CDS             complement(7135..7593)
                     /gene="decR"
                     /locus_tag="OO468_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR6259484.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO468_002401"
     CDS             complement(7747..8565)
                     /gene="cof"
                     /locus_tag="OO468_002401"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR6259485.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8665..10357
                     /locus_tag="OO468_002402"
                     /pseudo
     CDS             8665..10357
                     /locus_tag="OO468_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10422..11117
                     /gene="queC"
                     /locus_tag="OO468_002403"
     CDS             10422..11117
                     /gene="queC"
                     /locus_tag="OO468_002403"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR6259486.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OO468_002404"
     CDS             complement(11169..11567)
                     /gene="fadM"
                     /locus_tag="OO468_002404"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR6259487.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11661..12032)
                     /locus_tag="OO468_002405"
     CDS             complement(11661..12032)
                     /locus_tag="OO468_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR6259488.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OO468_002406"
     CDS             complement(12183..14054)
                     /gene="ppiD"
                     /locus_tag="OO468_002406"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR6259489.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OO468_002407"
     CDS             complement(14246..14518)
                     /gene="hupB"
                     /locus_tag="OO468_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR6259490.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OO468_002408"
     CDS             complement(14727..17081)
                     /gene="lon"
                     /locus_tag="OO468_002408"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR6259491.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OO468_002409"
     CDS             complement(17269..18543)
                     /gene="clpX"
                     /locus_tag="OO468_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR6259492.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OO468_002410"
     CDS             complement(18669..19292)
                     /gene="clpP"
                     /locus_tag="OO468_002410"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR6259493.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OO468_002411"
     CDS             complement(19539..20837)
                     /gene="tig"
                     /locus_tag="OO468_002411"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR6259494.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OO468_002412"
     CDS             complement(21181..21498)
                     /gene="bolA"
                     /locus_tag="OO468_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR6259495.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21803..22381
                     /locus_tag="OO468_002413"
     CDS             21803..22381
                     /locus_tag="OO468_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6259496.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22425..23900
                     /gene="ampG"
                     /locus_tag="OO468_002414"
     CDS             22425..23900
                     /gene="ampG"
                     /locus_tag="OO468_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR6259497.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5667 a         6666 c         5828 g         6025 t
ORIGIN      
        1 ccgggcggag agggttaacg tcgcttaata ttgcgtagga gcgggaactg ccgaagacgg
       61 attcacctga gttggtgaag tcgaaggtac ggtagtcgtt gcgccaccgc tggcctgaac
      121 cggcacggcg gtttgctgta ccggcaccaa tgtcaggtcg gctttagttc cgccctggtt
      181 gatcaccggc tgaacggtat cggtgataaa taccagtttg tcattcacgg taatcgccgc
      241 actcaacaga atacgcgcgt tcggctgaac atctgccggg ttaaatggca gaacaaagct
      301 gaatggtgac tgtttacctt cagtacgcac cgctttctgc gccagcactt ttgacggtgc
      361 atcggctaac gacgcgtcag aaagtgtcac ggtcagcaca gcatcaggcg gcagtgcgac
      421 tttctgacgg atccagacag taccggagac attcggttgc tggatagcag gttgttgtgt
      481 tgctgaaata gacgtatttg cagccggggc tggcgtctga atatccgcgc ttttatctgc
      541 gcaagccgcc aacgcaatcg caaccgctaa accactggcc atgtgcacga gtttcattca
      601 tttctcctta ttatcaatgc accagcgggc taactttcct cgccggaaga gtggttaaca
      661 aaatagtaac gtcaacaagt gtggcacaca tcacgcattt ctgcctgtaa ttagcccgta
      721 attcagacta tttcacccat cggaccagtg gcaaatttgc gttatactca actcactttg
      781 gcttgctgcg gcagctttgt tactggagag ttatatgagt caggcgctaa aaaatttact
      841 gacattgtta aatctggaaa aaattgagga aggactcttt cgcggccaga gtgaagattt
      901 aggtttacgc caggtgtttg gcggccaggt cgtgggtcag gccttgtatg ctgcaaaaga
      961 gaccgtcccg gaagagcggc tggtacattc gtttcacagc tactttcttc gcccaggcga
     1021 tagtaagaaa ccgattattt atgatgtcga aacgctgcgt gacggtaaca gcttcagcgc
     1081 ccgccgggtt gctgctattc aaaacggcaa accgattttt tatatgacgg cctctttcca
     1141 ggcaccagaa gcgggtttcg aacatcaaaa aacaatgccg tccgcgccag cgcctgatgg
     1201 cctgccttcg gaaacgcaaa tcgcccaatc gctggcgcac ctgctgccgc cagtgcttaa
     1261 agataaattc atctgcgatc gtccgctgga agtccgtccg gtggagtttc ataacccact
     1321 gaaaggtcac gtcgcagaac cacatcgtca ggtgtggatc cgcgcaaatg gtagcgtgcc
     1381 ggatgacctg cgcgttcatc agtatctgct cggttacgct tctgatctta acttcctgcc
     1441 ggtagctcta cagccgcact gcatcggttt tctcgaaccg gggattcaga ttgccaccat
     1501 tgaccattcc atgtggttcc atcgcccgtt taatttgaat gaatggctgc tgtatagcgt
     1561 ggagagcacc tcggcgtcca gcgcacgcgg ctttgtgcgc ggtgagtttt atacccaaga
     1621 cggcgtgctg gttgcctcga ccgttcagga aggggtaatg cgtaatcaca attaaaaaaa
     1681 tagccggagg tgaaaaccgt ccggctattt tttgcagtgc ttgttacgcg ttataggcat
     1741 tctcgccgtg gctgttgacg tccagccctt ctcgctcttg ttcttccggc acacgtaggc
     1801 caaccgtcag atccgccagt ttgtagccaa taaatgccac cacaccggac cagacgatcg
     1861 taatggcgat gctttccaac tgtaccagca actgatggcc catcgtcaca ccttcagcga
     1921 agcccacgcc gcccagagaa ctggcggcaa aaatcccggt catgatacag ccgacaatgc
     1981 cacaaacgcc gtgcacaccg aagacatcgc agggatcatc cacccgcagc aagcgtttga
     2041 gcatggtaac gccccacaaa cccgccagac cagctaccac gccgataatc aacgcgccgc
     2101 caaccccaat gtagccgcac gctggcgtca cgccgaccag accggcaatc gcgccagaac
     2161 acgctcccag cagagaaggc ttaccacgca gcgcccattc accgaagatc cagccaagaa
     2221 ttgccgccgc cgttgcgacc acagtattca caaatgccag tgccgcgatt tcattcgccg
     2281 tgcccactga cccggcgtta aagccaaacc aaccgatata gagaatggca gtcccggtga
     2341 agaccatcgg caggttgtgc ggtttaaacg cctctttacc gaagcctacg cgtttgccta
     2401 tcagatacgc gcccaccaga ccggcgattg ctgcgttaat gtgcaccacc gtgccacccg
     2461 cgaaatccag cgcaccgtga gaagccagca aaccaccgcc ccacaccata tgcgcaatcg
     2521 gaatgtaaga gagcgtcagc cataccacca cgaaaatcaa cacagctgag aagcggattc
     2581 gttccgccag cgccccaact atcaagccta cggtaatgca ggcaaacgat ccctgaaacg
     2641 ctacgtggat atactgataa atgctgccca tcaccgccgt cagttcgatg ttttttagca
     2701 tcaaccagtt aatgttgccg aagaagttgt tgccctcacc aaacgccagc gaataaccgt
     2761 aaaccaccca gagaatacag accagtgcaa acgtcaccgt cacctgcgtc agcatcgaca
     2821 gcacgttttt gccgcgaatc aacccaccgt aaaacagggc aatccccgga atagtcataa
     2881 acagcaccag cgcagtacaa atcatcataa acgcattgtc ggctttatcg gccaccgcag
     2941 gtgcagccat taccagtccc ggcagcatcg ccagtgaagc aagcccagtt tttatcgtcg
     3001 ctatcttcat ttttcgttcc tgttgctgtg tgccagagat tacagcgccg cttcgtcggc
     3061 ttcgccggta cgaatacgaa tgacgcgttg caattcagcg acgaagattt tgccgtcgcc
     3121 aatttttccg gtgtaagccg ccttactgac gatatcgatc acttcatcga gttggtcatc
     3181 agcaatcgcc acatcaattt ttacttttgg caggaagttg acgctgtatt ccgccccccg
     3241 gtacagctcg gcatgccctt tctgacgccc gaaacctttc acttcggtga cggtcaggcc
     3301 ctgaatacca atggaagata acgcttcacg aacgtcttcc agcttgaatg gtttgattat
     3361 cacggtcacc agcttcatag atcccctccg gtcagaattc ggtaatggtt ctgctacacg
     3421 aagaaggtat tgcaagcggt gtgccagaaa tgaaaaacga gaaagagagc aggaagttaa
     3481 gaagattgaa aatgaaaacg cactatgaca gtgcacagtg cgttacattt ttgcaccaac
     3541 gatgaaatat tgcgctattc aggcgctcaa tgattcctct tcacgcacgc tggctgccag
     3601 ctcttcgccc gcaagttgca gttgatacat ctgccagtag cgtccctggg ccgccagcag
     3661 ttgctggtga gtgccctgct ccacggcttg cccacgatga agcaccagaa tggtgtcggc
     3721 atcaataata gttgataagc ggtgagcaat caccaccagc gtggtatgtt cacgcaccgc
     3781 cgccagagca tgttgaatcg cctgttcagt accggagtca atgctggcgg ttgcctcatc
     3841 aaggatcagg atttgcggcg tctccaccag cacgcgcgcc agtgccagca gttgcttttg
     3901 cccgactgag agattatttc cctgctcgcc cagcggcgta taaatacaat cactcatgct
     3961 gcgcgccagc tccgccagtt gcacggtttc cagcgcctgc cagacgcgtt cttcggagat
     4021 atcccgcccc agcgtcacat tagcgaggaa ggtatccgcc agcaccaccg gatcttgctg
     4081 caccattgcc acgccctggc gcagcgcgct atgacttagc gaacttaatg gacgaccatc
     4141 aaggtgaatc tcaccttccg ttagcgggta atagcccatc aataaactgg cgagggtact
     4201 tttaccactg ccggtatgcc cgaccagcgc cacaaagtta cgcgaaggca cagagagatt
     4261 aatgttcttt agcaccagat tgtcatcgcg ataagcaaat gacacgttat cgacttcgat
     4321 ggtgccactc tgtaacgggt aatcatcatt gccatattgc tggcgcggtc cgtccatcag
     4381 ttcaaacacg cgctcaccag caacaacagc ctgttgcagc atcgcctgtt gcgtggtcag
     4441 ttcgattaag ggttcgttaa gtcgcccaag atagctgata aacgcataca acacgcccac
     4501 ttcaatggtg ccgctggcgg agaagccaaa cagcatcaat aagccacaaa gaatgagcga
     4561 tgaaaacaga ctcagcagcg gacgcagcag aaaaccgtcg aggcgcaggg tttgcatcct
     4621 agccatatag tgtgaacgac tggcctcccc catacgttcg ccaaatcgcg cctgctgacg
     4681 aaactgctgg ataacgctca tgccattgat gatttcgtta aagccgtcgt tgatatccgc
     4741 cagataggcg cgcacacggc ggacaatcgg cgtgctgtaa cgctggtata tcaccattac
     4801 caccagcacc accgggaaaa tcattatcgc caccagcgcc attcgccagt cgaggctgaa
     4861 catcgccacc agcatcgcgc ccaccagtgc ggcactgcgc aggacagttg ccactacggt
     4921 aacgtagaga tcgcggatca cttcagtgtc attagtgacg cgggaaatca cctgcccgac
     4981 gggttgggta tcaaactcgc ttaatggctg gcgtaacgca gcatccatca cgtcggtacg
     5041 caactgttgc actgcgccta ctgccgcccg attaaacagc agcgactgcg cgtaatgtag
     5101 cccggcggca aacagttgta gcccaacata cgccgcagcc agccctgcaa ccactttcaa
     5161 cggcaggtta tttttcgcta ccatattgtc gataaaatag ctgataagca gcggcccact
     5221 gacttctgcc gccgccgcaa cccacatcat caggaccgca atccccagcg gtttacgcca
     5281 cggcgaacca tacgctaaca ggcgcttgag agtcggccac agttggctaa aactacgcat
     5341 cgacggcctc ctcgcgaatt tccggaacgt cgtcgagcgc cgcctccagt tgttgatagc
     5401 gatacatatc gcgataccaa ccgctttgtt gcgccagctc atcatgattg ccacgctggg
     5461 cgatatgtcc gtgctgcatc acaataattt cactggcttc cgtcagtgcc gaaaggcgat
     5521 gggcactgat gattaccgtt cttccctgtc cccactgacg caggttatgc aggatctggt
     5581 gctctgtgcg tccgtccacc gccgaaagcg catcatcaag gatgaggatt tccgcgttga
     5641 ctaataacgc tcgagcaatg gagatacgct gtttttgccc accggaaagc atcacgccgc
     5701 gctcgcccac ttctgtatcg taaccttgcg gtagacgcaa aatatcgtca tgtacgctgg
     5761 ctaaccgcgc gacatgctca atctcttgct gggtggcatt cgggcaaccc agcgcgatgt
     5821 tattcgccac ggtgtcagaa aaaaggaatg gcgtctggct gaccaccgcc aggcggctac
     5881 gccagctatc gagttgtaac ttcgtcagag gaatatcatg aaagcgaata tccccctcgc
     5941 tgacgtcgaa atgacgctga atgagcgaca acagggtact tttgccggaa ccagtcggcc
     6001 cgcaaatacc cagcatctga ccgggtttca gggcgaaatt gacgttttcc agcgcaggat
     6061 ggtcagtctg cggatacgtg aactggtgaa tatttacatc cagttcgcca cgcccttccg
     6121 gcacgggttc gctaccatcg ttcaccaccg gcgcttccgc cagcatggcg cgaatacggc
     6181 tgtatgcagc actaccacgt tccacaatgt taaacatcca tgccagcgcc agcattggcc
     6241 aaatcatcag acctaaatac atcataaaac tggtgagctg gcccagcgtt aaactgccct
     6301 gcaccaccat ccaactaccg ccgccaatcg ccagcaagtt cgccatacca atcgcgatat
     6361 agatggtcgg gtcgaagcga gcatcaatac gcgccacccg catgtttttt ttgccggtat
     6421 cttcggcatc cgcggcaaat aacgccgact ggcgatcttc cagaccaaag gctttgatca
     6481 tgcggatact ggtgaggctt tcctgggtgc ggtcattaag actggaaaac gccgcctgtg
     6541 ccagcttaaa gcgttcatgc aaggcatcgc cgttgcgctt aatcatgatc gccatcactg
     6601 gcatcggtaa cagggcaaat aaggtcaact gccagctaat ttgcgtagac atcataatca
     6661 acacagcgca gcccattacc agtgaatcca ccagcgtcag caccccttct ccggcggcaa
     6721 acacgacgcg atcgacgtca ttggtcgcac gagccatgag atcacccgtg cgatgacgca
     6781 ggtaaaactc cgggtgctgc cggcttagct gacggtaata atcttcacgc agttcaacag
     6841 ccagttggta agacgcgcca aacagcaata cgcgccagac ataacgcagg agataaacca
     6901 caacggcaat cagcaccatg gtggcgatcc acatcaggct cagcccggta gtaaagtgtt
     6961 gttctgtcac gccatcgaca acaataccaa ccacttttgg cggaaccagt tgcagcatcg
     7021 cgataatgac aagcaaggcg acagccccaa gatagcgacg ccattcccga cggaaatacc
     7081 agcttaattg agcaaataat cgcacgcgtt gtgtcctgac ctgattctgg atatttattc
     7141 gatgggtaaa gaagtggtgt atttaatctg ttccatcgcg aagctggaag tgacgtccga
     7201 cagccccggc acgctgttta ccagacgctt ataaaactcg tcgtagcgtt tcatgtcggc
     7261 aacctggacg cgcatcagat aatcgtattc accagccatg cgccagaccc ccagcacttc
     7321 tggcatttcg gtaaccaccg tgacaaagcg gcaataccat tcgctgctgt gatgttgcgt
     7381 ttttatcagc acaaaggcgg tcaggccgag gcctattttt tccggatcca gcagggcgac
     7441 tttgccgata aggataccgt cgtcctccag ccgtttcagg cgcttccagc agggggtggt
     7501 tgtcagatta acggcttcag ccagtgcctg caaagagagg gtgcaatcct gctgcagtaa
     7561 ggccagcagc ttacggtcaa ttttatctaa catagccctt ccacggagaa tttttttctc
     7621 ttttaattct attttaaagg tgaaatcgcg acaatttatt ctgtgcggtt taaacaccac
     7681 ggcacaaaat gacaaattca acgcaaaaaa aaaccgggca attgcccggt gctggaaggg
     7741 atctcgttat tcgggggaat aaggtagatg tggatagtcc agccagtgcg tcaaatagtg
     7801 agagacagcc tgatttcggc aatgtccaat caccggtaaa tgcgggagct ccgcgcgcag
     7861 ttgcggcatc gcattgccca taataaaccc gctaccgacg ctgcctaaca tttcgcgatc
     7921 gttcatcgca tcaccaaagg ccatgcaatc gcgcaacgat aaacctaaat gttgggtccg
     7981 caccgtcaat gcagcgcctt tattgcagcc caccggcagc acttcgaggc aatccgtggc
     8041 ggaaaaacac aaatgtgcac gctcgcctaa tgcttcgtat agctggatct gcaagcgtgt
     8101 aagatcgtcg tgatcgccac agaagcagat cttggtgacg ctgccgagtg gcattttttt
     8161 gacatcgatt atctgataac gaaaaccgct atagacaaat acctgcaaca acgcagggct
     8221 ctctttcccg gtaaaccaac cgtcgtcatt gaagatatgc atgctggctc gggtatccca
     8281 ttgctgatac agcaccagct ccgcgacatc cgcaggtaaa tcatcacgat gtaaaagttc
     8341 accttccaga gaatgcacgc gcgttccgtt gccggtaatc aaatatgcat ccagcgatag
     8401 cgccccgaga atatgctgca tctccagcgc atgacgcccc gtggcaaaag tgagggtaat
     8461 gtcgcgttca cgcagtcgcg ccaaagtaga gagggttttc tcacctaaat gatggtcagg
     8521 catcaataaa gtgccatcca tatcaaatgc taccagacga gccatttctt tctccactct
     8581 gtgacacggt taatggtgat tgagtatcac ctgatatata cggaagtaat agtgaatagt
     8641 taaataatat tgttccgggt ttatatgcga ttgctcaacc gtcttaacca gtatcaacgt
     8701 ctgtggcaac cttcagccgc aaaccgtcac cgtcagcgaa ctggccgaac gctgtttttg
     8761 cagcgaacgc catgttcgta cgctgttgcg tcaggcacag gaggcgggat ggctggagtg
     8821 gcaggcgcag tcaggacgcg gaaagcgcgg acaattacgc tttctggtca cgccggaatc
     8881 gctacgcaat gcgatgatgg aacaggcact ggaaaccgga aagcagcaag atgtgctgga
     8941 gctggcgcaa ctggccccag gtgagctgcg cactctgtta cagccattta tgggcggaca
     9001 atggcaaaac gatacaccca cgttgcgtat tccctactat cgcccgctcg aaccgctaca
     9061 accaggcttt ttgcccggcc gtgccgagca gcatctcgcc gggcagatat tttccggcct
     9121 gacccgcttc gataataata ctcagcgccc gattggcgat ttagcgcatc actgggaaac
     9181 ctctactgac aggttacgct gggactttta tcttcgttca accctacact ggcataacgg
     9241 cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac aactgccagc
     9301 actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc agtgtctgac
     9361 cttcttttta catcgccccg attactggct tgcgcaccgg ctggcgagct attgcagcca
     9421 tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa cacaattcac
     9481 agcggagctg gtgcgcctgg agagccatga ttattaccat ttgcgtcatc cgctgcttaa
     9541 agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa ccagttgtcg
     9601 gcatcccgtg caaatcacca tcggcaaacc ggaggagctg caacgggtca gccaagtcag
     9661 tagtggcatc agtttaggtt tttgctattt aacgttgcgc aaaagtcccc gactctccct
     9721 ctggcaggcg cgaaaagtga tctccattat tcatcaatcc ggtttattac aaacgttaga
     9781 agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga ctattccgca
     9841 ctggcaagta ccggatgaag tcaaactacc gaaaaccttg acgctggttt atcacctacc
     9901 gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg aaggctgtga
     9961 actcacaatt atttttcata acgcaaaaaa ctgggacgac acgaccctac tggcacacgc
    10021 agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatatactc tggagcaatg
    10081 gctacgttgc gatccactat ggccacatgt tttcgacgct ccagcatatg cacatctgca
    10141 atcgacgctg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta atgccctgaa
    10201 agcggttttt agccagttaa tggcagacgc gacgctgacg ccgctgttca actatcacta
    10261 tcgcattagt gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg gctggtttga
    10321 atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg gttaatcgca
    10381 ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt gctgtcgttg
    10441 tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa caatatgatg
    10501 aagtccattg cgtgacgttc gattacggtc agcgacatcg cgcagaaatc gacgtggcac
    10561 gcgaactggc gctgaaactg ggagcacgcg cgcataaggt tcttgatgta acgctgctca
    10621 acgagctggc ggtcagcagc ctgacacgtg acagcattcc ggtgcctgat tatgaacctg
    10681 aagccgatgg catcccgaat acgtttgtcc cagggcgtaa tattttgttc ctgacgctgg
    10741 cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc tgtgaaacgg
    10801 atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac catgccgtca
    10861 gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt gataaagcgg
    10921 aaacctgggc gctggcagat tattacggca aactggattt agtccgtaac gaaacgttga
    10981 cctgctataa cggcattaaa ggcgacggtt gcggtcattg tgcggcatgt aatttacgcg
    11041 ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg atgaagcaga
    11101 aaaccgggtt gaggtaatta ttccgggtgt cgccggatgc ggcttgagca tccggcacca
    11161 caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc cagagctaat
    11221 gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc taccacctgc
    11281 ccttccggct ccagtgtaat gacctggttt aagatgccgc ttttaccgtt taattgctgc
    11341 aactgactgg taatagttaa caggtcgctt aataccgctg gacgacgata gttaatattg
    11401 atattgacca cgacgaaggc gatgttatgg gccgtcatcc actgaaaact gtcgctattt
    11461 tccaccccat cccagcgggc ttcttcgaga aattcaaggt agcgggcgtt gttgacgtgc
    11521 tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc gcaataacct
    11581 tacgttgttg ttataagtac agaggtcata actggtatga cctcttcagt ctggcaaaaa
    11641 attgccacta tgcaaattaa ttacagggtt aataccgcca gattacgttc caccagcgaa
    11701 ttgcccatcc ccggcacctg ctttagatcc tccaccgttt taaacggacc gtactcttcg
    11761 cgataactga caatcgcctg cgctttcttc aggccaacgc cattcatcgc gcgggctagc
    11821 tcttccgcgc tggcattatt aatgctgacc cgggtgcctt cttcgtcact ggctttcgcc
    11881 ggtactgctg ctttactttg tgctgcagga gcttccgctt tggtttctac cgccgtcggt
    11941 ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc cagggacagt
    12001 gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt gttaacagtg
    12061 gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa atggaaaagg
    12121 ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag gcggcttcag
    12181 gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg caggttactc
    12241 atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac catcgctttt
    12301 ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag aaccacatta
    12361 ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag tggcagtgca
    12421 aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg ctcgccaaat
    12481 ttcagaccgg cagcctgcat agcttccgca cctttgccgg ctttcaaatc aaccagcagt
    12541 ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag tgccttaact
    12601 tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct gacgcgcagc
    12661 acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccga cgcgccgttt
    12721 tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt caactcttcc
    12781 ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc agcttgctct
    12841 gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg ctgcagcgca
    12901 tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc acgtacttcg
    12961 tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag gaaaccgacc
    13021 gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt tttcagttcg
    13081 tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc agagataata
    13141 tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag ctcatcaagt
    13201 accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt acgctgcggc
    13261 tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc gctaaccggt
    13321 tgctgcatcg ttgcggcaac cagcttgatg taactcacgc ggaattgttc cggcgtcatg
    13381 aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac aggctgcttc
    13441 gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg cgcgaccagt
    13501 gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac gccgttaatc
    13561 agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc ggtcatcccc
    13621 atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac ctggaaggct
    13681 ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt cagctcacga
    13741 gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg acgcagggtt
    13801 ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg ttgctgcata
    13861 cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc ctggtcattc
    13921 acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc ggtcaatatg
    13981 aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc agccgtgcgt
    14041 aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc tcacgcaacg
    14101 tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca gcccacaaga
    14161 cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc cttgaacttc
    14221 gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct ttacctgcac
    14281 ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc gggttgcggc
    14341 cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt gctacatcat
    14401 ccccttcttt cagagattca gttacggaag caataatagc atctaacgca cggccagccg
    14461 cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat ttattcactc
    14521 ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga acgcgcagca
    14581 gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc ccgatccgct
    14641 atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc agccctgttt
    14701 ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat gccagacggt
    14761 tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg aatgtccaga
    14821 tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa cggaattagc
    14881 actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc accgatcggc
    14941 agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg aaccgggtta
    15001 ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg accatctttc
    15061 ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa atcagggttg
    15121 atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat agactcctgc
    15181 atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac acatgcggtt
    15241 tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccgattac ctgaccgaca
    15301 cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta gtcatgcagg
    15361 ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg cttaaccgct
    15421 ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc acgggtgtag
    15481 taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt tttcagtgca
    15541 ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt ttcatcttcg
    15601 gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg aatgttcatg
    15661 gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc caggtagtgg
    15721 tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga ggccggatcg
    15781 cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg atttttcacg
    15841 cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta agtacggcgg
    15901 tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac atatttacgc
    15961 ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg cggccctacc
    16021 aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag gtactcaagg
    16081 atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc ctgcgcctga
    16141 cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat ccagtcgata
    16201 taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag cttctgcaac
    16261 tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat tttgcgcttc
    16321 agggcttcgt tttcgtccgg cacgtcgtcc atttcaccga gttctttctg aatagctttc
    16381 atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt aacgcggttg
    16441 cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc catcagatat
    16501 tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc cagtttcagc
    16561 ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct attcagcgac
    16621 gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa ctggctgatt
    16681 gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc cagatactcc
    16741 gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc gcgctgtaac
    16801 ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa tatagaggcc
    16861 acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc ttctttctgc
    16921 gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg gatagatttt
    16981 tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc gcgcagcggc
    17041 aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc tcttagttta
    17101 atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta acatgtacgt
    17161 cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa attaaagggg
    17221 agataaaatc cccccttttt ggttaactaa ttgtatggga atggttaatt attcaccaga
    17281 tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac catcaattac
    17341 cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt acatggtatc
    17401 gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac gcgccatcgc
    17461 tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc cttccagatt
    17521 aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga tctgaatcag
    17581 agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa tgaactcagg
    17641 gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct cgccttcgct
    17701 tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc cggtttctac
    17761 acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa acaggatctt
    17821 agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt gcggtggaac
    17881 agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtactcctt caccggaaac
    17941 gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt catcgatgta
    18001 gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca gcttctgaat
    18061 gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg cgtcggccat
    18121 ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt taccggaacc
    18181 ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc tggtatcgcc
    18241 gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt tcgcctgttc
    18301 ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg gtagcgcact
    18361 gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt tacataaatc
    18421 aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca cttcatgctg
    18481 gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc gtttatctgt
    18541 catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa atgccattct
    18601 caggcgcaag ccgcttacag cgttgtgccg ccctagataa gtatagcggc acagttgcgc
    18661 ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc caccgcttca
    18721 ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa tgattgaccc
    18781 gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc acgggcatga
    18841 atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat catcacgcgc
    18901 gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcaacaagaa agcgcccatc
    18961 gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg catggtgtca
    19021 tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag atagatatct
    19081 ttttctgggt tttccgcttc caggaacagc atctgcgcca caatcaggtt agccatgtgg
    19141 tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga atagatatca
    19201 aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc catatggggt
    19261 gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa attgaaaaaa
    19321 acctgctgta ccgattgtaa ccttatggac ggattatcag ctagtccctg tgttatgggt
    19381 acggcatcac catatttcaa gcataacaat cttttgttgt tacgctaaca ccgaaagagg
    19441 cttttcgcac ggctccatgc aaaattcatg acaaaaaaaa gcccaccacc tggcggtgac
    19501 gggcctttgt gcgaatttag cgcgttatgc tgcgtaaatt acgcctgctg gttcatcagc
    19561 tcgttgaaag tggtttcttt ttcagtcact ttcgctttcg ccagtacagc ttcaacagcc
    19621 tgttcttcca gagcaacatt gcgcatgttg tccatcagtt ctttgttttt gctgtagaac
    19681 tcgataactt ctttcggatc ttcgtacgca gaagccatct cttcgatcag gcctttcacg
    19741 cgctcttcgt cagctttcag ctcgttggtg cggataactt cgcccagcag caggccaaca
    19801 actacgcggc gtttagcctg ttcttcgaac agttcgcgcg gcagttccag agcttgtttt
    19861 tcgttgccac cgaaacgctg tgcagcctgg cgacgcagaa cgtcgatttc gctgtcgatc
    19921 agcgcagccg gtacgtcgat gtcgttagct tttaccagac cttcgatcgc ctgagactta
    19981 acgcggttac ggatggcgct cttcagctcg cgctccatgt ttttacgcac ttcagcgcgc
    20041 agaccttcta cggaaccatc ttcaacgccg aaacgtttga tgaattctgc agtcagttcc
    20101 ggcagttcac gctcttcaac tttcttcagg ttgatagcga atttcgctgc tttacctttc
    20161 aggttttctg cgtggtattc ttccgggaag gtcacgtcga tggtgaactc ttcgccagct
    20221 ttgtggcctt tgataccgtc ttcaaagccc gggatcatac gaccctggcc catcgccagt
    20281 acgaaatcag acgctttacc gccttcgaac tcttcgccgt ctacagaacc ggtgaagtcg
    20341 atggttacgc ggtcttctgc ttcaacagcg ccgtcttttt ctttccaggt cgcctgctgt
    20401 ttacgcagag tatccagcat gccgtcaacg tcagcgtcgg tcacttcaac gatcggtttt
    20461 tcaacttcga tcgcttccag accctgcagt tcaacttccg gataaacttc aaactctaca
    20521 gagtaagtga agtcttcacc cagcttgtat tcgcccggaa cataagtcgg tgcgccagcc
    20581 ggattgattt tttctttaat gatggcgtca atgaagttac ggctcatcag gtcacccaga
    20641 acgtcctggc gtacagacgc gccataacgc tgagcaacga tattcattgg cactttgcct
    20701 ttgcggaagc cgtcaatacg tacttttttc gcaacgttga ccagctcgct tttaacagcg
    20761 gtctcgatgc tgtcagcagc gatagtaatc gttacacggc ggccaaggcc ttgagtggtt
    20821 tcaactgaaa cttgcatctt gttacctcaa aaaatcacag tgctcggtca actcttaccc
    20881 cgcaagcaca ggttgttggc ttcgcggaac cgggatgttc ttataatcaa tcacattccc
    20941 tgttaccaga atcatcccga aagcattcaa ataggacgcg gcattatagc ggcatcactt
    21001 ttatgagtcg agaacggtta ttgcgcgctg tcgcgtattt tttcacaatt cccgggcaat
    21061 tttcgtctga atgctgggca aaaagcgcaa aatccagaca aaacaaaacg gcccgcaggc
    21121 cgtttttcat aaaatcttta gcaacatact ggaaaagcgc cgacagttgc aaatgcgttt
    21181 ttacgcgatg cttcctgctc cacgacaggg aggagaggca aagacggtgt cctgcaaccc
    21241 ttcccactcc ttaatagtgt aagtatgcag agccagcgca tgaacggtag tagagagttc
    21301 ctccgctaaa gtactgtaaa tcattcgatg acgattcaga aaacgttcac ccgtaaaacg
    21361 atcgctgacc agcacaactt taaaatggct ttcagagccg gctgggacat tgtgacgata
    21421 gctttcatcc actacttcga ggaatacggg ttggaacgcc gcccttaatt tttcttctat
    21481 ccgctcacgt atcatcatga aatatctcct ccgacaacgc tgagatgtca tccatccctt
    21541 taaatactag ccgcttttac cgtttccatt gcagcttaac aacaaatatt taggtttcgt
    21601 cctggttttt ccgtcaagcg tatgaagttt actgatttaa agattgaaac acaggcctcg
    21661 catcagcgtt tcttttacca ggggtgatta tttcgtcaac atgatgaatt tacatgcctt
    21721 acccacttcc cctcgccgtt ggcaatgtta tgatggcggg aatttttacc aaacacgctt
    21781 aagagtctct gagagcccga acatgttcaa aaaaatcctc ttcccgttag ttgctctgtt
    21841 tatgcttgca ggatgcgcaa aaccgccaac aactattgaa gtttccccga cgattacact
    21901 gccacagcag gatccaagcc tgatgggcgt caccgtaagc attaatggtg ccgatcagcg
    21961 taccgatcag gcgctggcaa aagtcacccg cgataatcaa atcgttaccc tgaccgcctc
    22021 ccgcgctctg cgtttcctgc tgcaagaagt gctggaaaaa cagatgaccg cgcgtggtta
    22081 catggttggt ccgaatggcc cggttaatct gcaaatcatc gttagccaac tgtatgctga
    22141 cgtgtcccag ggcaacgtgc gctacaacat cgctaccaaa gcggatatcg ccatcatcgc
    22201 taccgcgcag aatggcaaca aaatgaccaa aaactatcgt gccagctaca acgttgaagg
    22261 tgcgttccag gcctccaaca aaaatatcgc cgatgcggtt aacagtgtgc tgaccgatac
    22321 catcgctgat atgtctcagg acaccagcat tcacgaattc atcaagcaga acgcgcgtta
    22381 atttctgccc tctggcccgg tgcaagccgg gcctgtagac gcccatgtcc agtcaatatt
    22441 tacgtatttt tcaacagccg cgttcagcca tattgctgat cctgggtttt gcttccgggc
    22501 taccgctcgc cctgacatcc ggcaccttac aggcctggat gacggtcgaa aatatcgatc
    22561 tcaaaaccat tggtttcttc tctctggtag gccaggctta cgtttttaaa ttcctctggt
    22621 caccgctgat ggaccgctac acgcccccat tttttgggcg gcggcgcggt tggctgctcg
    22681 ccacgcaaat cctgttatta gtcgccattg cggcgatggg ttttctcgaa ccaggcaccc
    22741 aactccgctg gatggcggcg ctggcggtgg tgatcgcttt ttgctctgcc tcccaggata
    22801 ttgtcttcga tgcgtggaaa accgatgtgc tcccggcaga agaacgtggt gcgggcgcgg
    22861 caatcagcgt gctgggttac cgtttaggga tgctggtttc cggcggccta gccctgtggc
    22921 tggcagataa atggttgggc tggcagggca tgtactggtt gatggcggcg ctgttgatcc
    22981 cctgtattat cgcgacgttg cttgcaccag aaccaaccga caccattcct gtgcccaaaa
    23041 cgctggaaca agcggttgtt gcacctctgc gagatttctt tggtcgcaat aatgcctggc
    23101 ttattttgct tcttatcgtg ctgtataagc tgggcgacgc attcgccatg agcctgacaa
    23161 ccacgttttt gattcgcggc gtcgggtttg atgcgggtga agtaggcgtg gtaaacaaaa
    23221 cgcttggctt actggcgacc attgttggcg cattgtacgg tgggattttg atgcagcgcc
    23281 tgtcgctgtt ccgggcgctg ctgattttcg gcattttaca aggtgcgtct aacgctggtt
    23341 actggctgct gtcgattact gataagcatc tctacagcat gggcgcagcc gtctttttcg
    23401 aaaacctctg tggcgggatg ggcacatcag cctttgtcgc gctgttaatg acgctgtgta
    23461 ataagtcatt ttccgctact caatttgccc tgctctcagc gctttccgct gtagggcgag
    23521 tttatgtcgg ccccgtggcg ggttggtttg ttgaagcaca cggctggtcg acattctatc
    23581 tattctccgt cgccgctgcc gtaccggggc ttattttgct gctggtttgc cgccagacgc
    23641 ttgaatatac acgagtaaat gacaacttta tctcccgtac cgaatatccg gcaggttatg
    23701 cctttgccat gtggacactg gcggcgggcg tcagcctgtt ggccgtgtgg ttacttctgt
    23761 tgacgatgga cgcgctggat ttgacgcact tctctttcct gcctgctctg ctggaagtcg
    23821 gggttttagt cgccctttct ggcgtcgtgc ttggtggttt gctggattat ctggcgctac
    23881 gaaaaacgca tctgacgtaa tctgtaaata ttatttagct gtagtaatca ctcgccgtaa
    23941 ttattacggc taaataaaca tcagcatcgc ttattaatat ttgttctttt gtgcggctta
    24001 gcgtttggaa attattgacg ccatttataa attctatttt tcttacacga ttcagctaat
    24061 gagtctttat tttctcacca cccagttgtc actttaatga taattatttg ttaaataatt
    24121 gttttatttc gcattggtta taccaattgc ccaccaagac tccttctgca ctcccctttt
    24181 gttata
//
LOCUS       DAJTUP010000059        24005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTUP010000059 DAJTUP010000000
VERSION     DAJTUP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.059"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..94
                     /gene="yjbD"
                     /locus_tag="OO468_002415"
     CDS             <1..94
                     /gene="yjbD"
                     /locus_tag="OO468_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR6259498.1"
                     /translation="ARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(227..1099)
                     /gene="rluF"
                     /locus_tag="OO468_002416"
     CDS             complement(227..1099)
                     /gene="rluF"
                     /locus_tag="OO468_002416"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR6259499.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1447..2394
                     /locus_tag="OO468_002417"
     CDS             1447..2394
                     /locus_tag="OO468_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR6259500.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2464..3270
                     /locus_tag="OO468_002418"
     CDS             2464..3270
                     /locus_tag="OO468_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6259501.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3280..3687
                     /locus_tag="OO468_002419"
     CDS             3280..3687
                     /locus_tag="OO468_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR6259502.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3687..4181
                     /locus_tag="OO468_002420"
     CDS             3687..4181
                     /locus_tag="OO468_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR6259503.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4247..5044
                     /locus_tag="OO468_002421"
     CDS             4247..5044
                     /locus_tag="OO468_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR6259504.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5055..5843
                     /locus_tag="OO468_002422"
     CDS             5055..5843
                     /locus_tag="OO468_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR6259505.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5895..7124
                     /gene="sorE"
                     /locus_tag="OO468_002423"
     CDS             5895..7124
                     /gene="sorE"
                     /locus_tag="OO468_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR6259506.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7506..8195
                     /gene="pepE"
                     /locus_tag="OO468_002424"
     CDS             7506..8195
                     /gene="pepE"
                     /locus_tag="OO468_002424"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR6259507.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8286..9917)
                     /locus_tag="OO468_002425"
     CDS             complement(8286..9917)
                     /locus_tag="OO468_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR6259508.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10137..13820)
                     /gene="metH"
                     /locus_tag="OO468_002426"
     CDS             complement(10137..13820)
                     /gene="metH"
                     /locus_tag="OO468_002426"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR6259509.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14020..14844
                     /gene="iclR"
                     /locus_tag="OO468_002427"
     CDS             14020..14844
                     /gene="iclR"
                     /locus_tag="OO468_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR6259510.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15161..17345
                     /gene="espL4"
                     /locus_tag="OO468_002428"
                     /pseudo
     CDS             15161..17345
                     /gene="espL4"
                     /locus_tag="OO468_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17314..19051)
                     /gene="aceK"
                     /locus_tag="OO468_002429"
                     /pseudo
     CDS             complement(17314..19051)
                     /gene="aceK"
                     /locus_tag="OO468_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19235..20539)
                     /gene="aceA"
                     /locus_tag="OO468_002430"
     CDS             complement(19235..20539)
                     /gene="aceA"
                     /locus_tag="OO468_002430"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR6259511.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20569..22170)
                     /gene="aceB"
                     /locus_tag="OO468_002431"
                     /pseudo
     CDS             complement(20569..22170)
                     /gene="aceB"
                     /locus_tag="OO468_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22439..23368)
                     /gene="metA"
                     /locus_tag="OO468_002432"
     CDS             complement(22439..23368)
                     /gene="metA"
                     /locus_tag="OO468_002432"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR6259512.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23525..23968
                     /locus_tag="OO468_002433"
     CDS             23525..23968
                     /locus_tag="OO468_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR6259513.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5910 a         6129 c         6089 g         5877 t
ORIGIN      
        1 agcccgccaa ttgaagaaaa agcaggctta taaaccggat cctgaagcgt catttagctg
       61 gtcagcaaat acgtcgacgc gcggcaggcg ctaatgaaca atcaacgcct gagattttca
      121 ctcgtctgtg tggacacaat cttcccctca ccctaaccct ctccccgaag gggcgagggg
      181 actgtccgag ctctttttgg acgttttcat cagtctgagg cgttaatcag cgtcccttct
      241 ttttacgccc cggcgaggta aaacgcttac cgttggacgc cgggcgaccg cctttttccg
      301 ccgttttttc catcttaacg actggacgtt tgatgcccgc tgttttcggt ttggccttcg
      361 ctttaggttt tgcctcggaa gaggaatttt cgatgagctt aaagaggtcg attaactcat
      421 cgtcggttaa atcgcgccac tcccccagcg gaatgccgct taagctgacg ttcataatgc
      481 gcgtacgttc cagctttttc acttcatagc cgaaatgctc gcacatgcgc cggatctgac
      541 ggttcagccc ctgcactagg gtaatgcgga agacaaacgg cgcttctttt ttgactttgc
      601 actttttcgt caccgtcccg aggatcggca cccccgcccc cataccacga ataaattcat
      661 cggtaatcgg tttatcgacc gtcaccagat actctttctc atgatcgtta cccgcacgga
      721 ggatcttatt caccagatcg ccgtgattgg tgaggaaaat cagcccctgg gagtctttat
      781 ccaggcggcc aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc
      841 gctcgccatc ttcggtggtg ctgacaatac cgacgggctt gttcagggcg ataagtacca
      901 aatcttcggc ttcccgaggc tcaatcaact gaccgtttac tttcacaatg tcgccgggtt
      961 tcacctgatc gccaatggtg gctcgcttgc cattaaggaa cacattgcct tgctcgatat
     1021 agcgatccgc ttcgcggcgt gagcaaattc cgctttcgct gatgtattta tttaaacgga
     1081 ctgatgagtc gggcagcata aattctcctg taaaacgcgg aatataccgt acctcttagc
     1141 ggggaaaaac agggttatgc ggcaacgtcg aaattaaatg tgatctgcca cgccttttat
     1201 cacaaaagtg tctccggaag tacgatgcaa atttgcctgg caaaataagg ataatcaaca
     1261 tattacgaat cgctgcacac ctgtgctgat gactactacg cagagaaaca aatgtgcaag
     1321 agaaagcgct atttctcccc atgcaaagag tgaaaaatcg ctttgaaagg ccctcatgat
     1381 ggggttatat cagatcaagc ggtgagaaag cgtatttgca caaatgtgca gaaccaggag
     1441 gcggcaatgg aaaacagtga cgatatccgt ttgattgtga agattgccca actctattac
     1501 gaacaggata tgacgcaggc gcaaatcgcg cgcgaactgg gtatttaccg caccaccatc
     1561 agccgcttgc ttaaacgagg ccgcgatcag ggaatggtca ccatcgccat caactatgac
     1621 tacaacgaaa atctctggct ggagcagcaa ctgaagcaaa agtttggcct gaaagacgtt
     1681 gtggtggtgt cgggaaatga tgaggatgaa gagactcaac tggcgatgat ggggttacac
     1741 ggcgcgcaac tgctggatcg cttgctggag cctggcgata ttgtcggttt ttcctggggt
     1801 cgcgcggtga gcgcactggt tgaaaacttg ccgcaggcgg ggcaatcgcg gcagttaatc
     1861 tgcgtaccaa ttattggcgg accgtccggt aaactcgaaa gccgctatca tgtaaacaca
     1921 ttaacctaca gcgcggcagc gaagctgaaa ggggaatcgc atctcgcgga ttttccggct
     1981 ctgctggata acccattaat tcgtaatggg atcatgcagt ctcagcactt taaaaccatc
     2041 tctgcctact gggataatct ggatatcgcc ctggtgggaa ttggctcacc ggccattcgc
     2101 gacggcgcta actggcatgc gttttatggt ggtgaagaga gtgacgacct gaatgcccgc
     2161 caggttgctg gcgatatttg ctcgcgcttt tttgatattc acggcgcaat ggttgaaacg
     2221 aatatgagcg aaaaaacgct ctctatcgaa atgaataaat taaagcaggc gcgatattcc
     2281 attggcattg ccatgagtga agaaaaatac agcggaatta ttggtgcact gcgtggaaaa
     2341 tatattaatt gtctggtaac gaatagcagc acagctgaac tattactgaa ataaaaatct
     2401 tcccgatgtt taccgtaata aaaattacgg cgggtcccta ttatctaaaa tcaggagtct
     2461 gttatgcaaa cgtggttaaa tttgcaggat aaaatcatta ttgtcaccgg cggcgcatcc
     2521 ggtattggtc tggcgattgt agaggaatta ttagcacaag gcgcgaatgt acagatggtc
     2581 gatattcacg gtggcgatgg tcaatatgaa ggtcataaag gctatcagtt ttggccgacc
     2641 gatatttcta gcacaaaaga ggtaaatcat acggtagcgg aaattatcca gcgttttggt
     2701 cgcatcgacg gtctggtcaa taacgccggg gtcaatttcc cgcgtctgct ggtcgatgag
     2761 aaagcgcctg ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat ggtcaatatc
     2821 aaccagaaag gtgtttttct gatgtcgcag gcggtggcgc gacagatggt caaacaacat
     2881 gatggcgtga ttgtgaatgt ttcctcagaa agtgggctgg aaggctcaga aggacaaagc
     2941 tgttacgccg ctaccaaagc cgcgctcaat agcttcacgc gctcctggtc gaaagagctg
     3001 ggtaaacacg gtatccgtgt ggtcggtatc gcgcccggga ttctggaaaa aaccgggcta
     3061 cgcacgccgg aatatgaaga ggcgctggcg tggacgcgca atatcaccgt cgagcagctg
     3121 cgtgaaggct ataccaaaaa cgccattcct attgggcgcg ccggaagatt agcagaagta
     3181 gctgattttg tttgttatct gttgtctgaa cgcgccagct acatcaccgg agtaaccact
     3241 aacattgcgg gcggcaaaac gcgcggctaa ggaggcttta tggtcaacgc cattttttgt
     3301 gcccacggca aattagcttg cgccatgctg gaatcggttc agatggttta cggcaacgcc
     3361 aatgttgaag ccgttgcatt tgtgcccggt gaaaacgccg gagacattgc ggtaaagctg
     3421 gaaaagttag taagcattca caatcaagat gagtggctga ttgccgtgga tttacagtgc
     3481 ggcagcccgt ggaatgcggc agcaatgctt gcgatgcgca atcctcgttt gcgggtaatc
     3541 agcggccttt ctctgccgct ggcgctcgaa ctggttgata accaggacag catgaatgtt
     3601 gatgaactgt gtgaacacct gacgcaaatc gcaaagcaaa cctgcgttgt ctggaaacaa
     3661 ctggcaacga cagaggagga tttctgatga acattacgct cgcccgcatt gatgaccgtc
     3721 tgatccacgg ccaggtcact accgtctggt cgaaagtggc taacgcccag cgcattatta
     3781 tctgcaatga cgaagtttat aacgatgaag tgcgccggac attattgcgt caggctgcgc
     3841 caccgggaat gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt tatcacaacc
     3901 cgcaatatca ggacgaaacc gttttttatc ttttcactcg cccacaggat gccttagcga
     3961 tggttcgcca gggggtaaaa ataggcaccc tgaatattgg cggcatggcc tggcggcccg
     4021 gtaaaaaaca gttaaccaaa gcggtttcat tagatgatga tgatattaac gcatttcatg
     4081 agttaaataa tcttggtgtc atactggatt tacgcgtcgt agcgtcagat ccctcaatca
     4141 atattatcga caaaataaac gaacagttaa tcgcaaatta aataatagcg cctgtgtaca
     4201 tatgctttaa caggcaggga tgacgacact taaaaggtga caaatcatgg aaataagtac
     4261 cctacaaatc attgctatat ttcttttttc ctgtattgcc ggaatgggca gcgtgctgga
     4321 tgaatttcag actcatcgcc cgttaattgc ctgtacggtg attggtttaa ttctcggtga
     4381 tttaaaaacc ggaataatac tcggcggtac gctggaattg atagctctct gctggatgaa
     4441 cgtcggcgcg gcgcaatctc cggattctgc actcgccagc ataatctccg ccattctggt
     4501 tatcgttggt cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg tggctgcggc
     4561 aggccaggtg ctgacggtgt ttgcccgtac catcaccgta gtgttccagc acgcggcgga
     4621 taaagcagca gaagaggcgc ggtttcgcac tctcgatatt ctgcatgtct ccgcgcttgg
     4681 cgtgcaggcg ctgcgcgtcg ccattccggc actgattgtc tcactgttcg tcagcgccga
     4741 tatggtcagc aatatgttaa acgccattcc cgaatttgtg acccgaggac tacagattgc
     4801 tggcggtttt atcgtggtgg tcggttacgc catggtgcta cgtatgatgg gcgtgaaata
     4861 tttgatgcct ttctttttcc tcggtttcct cgcaggtggc tacctcgatc tcagcctgct
     4921 ggcgttcggt ggcgtcggcg tgatcatggc cctggtctac atccagctaa atccacagtg
     4981 gtgtaaagct gaacctcaac tccaggccac cgcctccacc gcccttgacc aacttgacga
     5041 ttaacggagc catcatggaa cagagaaaaa ttacacgcag cgatctggtg agcatgtttc
     5101 tgcgctccaa cctgcaacag gcgtccttta actttgaacg tattcacggg ctgggctttt
     5161 gctacgacat gatccccgcc atcaagcgac tttacccatt aaaagaggat caggttgcgg
     5221 cgctcaggct acacctggtg ttcttcaata ccacgccagc cgtatgtggc ccggtcatcg
     5281 gcgcggaaat tgatgacggt accatcaacg gcatcaaagt cggtctgatg ggaccattgg
     5341 caggagttgg cgatccactg gtctggggaa cgctgcgccc gattaccgcc gcgctcggcg
     5401 catctctggc actttcgggc aacattctcg gcccgctgct gttcttcttt attttcaacg
     5461 cggtgcgtct ggcgatgaag tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata
     5521 tcgtcagcga tatgggcggg aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg
     5581 gactgtttgt gatgggcgtg ctggtgacca aatggacgtc aatcaacgta ccgttggtgg
     5641 tttcacaaac gcatgccgcc gatggctcca ccgtcaccat gaccgtgcag aacattctcg
     5701 accaactttg ccctggtttg ctggcgctcg gtctgacgct actaatggtt cgtctgctca
     5761 acaaaaaaat taacccggta tggctgattt tcgccctgtt tggcttaggg attatcggca
     5821 atgctctggg cttcctgtcc tgattcttcg ccccggcacg actgccgagg ccatcgctca
     5881 acatgaggtg gtttatgaaa acaacagctc tgcgtcttta tggtaaacgt gatttacgcc
     5941 tggaaacctt tgaccttcct gaaatgcagg aggatgaaat cctcgcaacg gtggtcactg
     6001 acagcctgtg cctctcttcc tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac
     6061 ccgacgatgt gacgaccaac ccaatcatca tcggccacga attttgcggc gatattctgg
     6121 ccgtgggtaa aaagtggcag cacaaattcc agccgggtca gcgttatgtg attcaggcca
     6181 acctgcaact ccccgaccgc ccggattgcc ccggctactc cttcccgtgg gtagacggcg
     6241 aagcgacgca tgtggttatt cccaacgagg tcatggaaca atattgcctg ctggcatacg
     6301 acggcgaaac ctattttgaa ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt
     6361 tcaacgccaa ctatcatctt caggaaggta gttataacca cacgatgggg attcgcccgc
     6421 aagggcacac gctgatcctc ggcggcaccg gaccaatggg actgttggcg attgattatg
     6481 cgctgcatgg accagttaac ccgtcgctgc tcgtcattac cgataccgac aacgacaaat
     6541 tgagttatgc gcgcaagcac tatccatcag aaccgcaaac actgattcat tatctcaatg
     6601 ccaccgatgc agcatttgat acgttaatgg cgctgagtgg cggtcacggc ttcgatgata
     6661 ttttcgtctt tgtgcctaat gaaggactgg tgactctcgc ctcttccttg ctggcgacag
     6721 atggttgcct gaatttcttc gccggaccgc aggataaaca tttcagcgcg ccaattaatt
     6781 tctacgatgt gcattatgca tttacccact acgtgggcac gtcaggcggc aataccgacg
     6841 acatgcgcgc ggcggtcaaa ctgatcgaag agaaaaaagt gcaggccgca aaagtggtaa
     6901 cacatattct tgggctgaat gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg
     6961 gcggcaaaaa gctggtgtat accgggaaat acctgccgct gacgtcactc acgcagattc
     7021 aggatcaagc actggcggcg attctggcgc gtcatcaggg gatctggtcc ggtgaggtgg
     7081 agcaatacct gctcgcccat gcagaggcaa tttcccatga ttaatcgcgc tacttaactc
     7141 tgcatgtcgc tggctggtcg ccccagcaac ttcgcgaccg gatgtgcggc tgattgctga
     7201 agcagcacaa tttgcacgag aaggataaaa aatatcagcg ccttaagggg cgctcaaatt
     7261 ggtggcaaag tacacattgt ttatgccgga tgcggaatga agccttattc ggtctacata
     7321 attttgaaaa tccaatatat tgcagttatc tggtaggcca ggtaagcgta gcgcatcagg
     7381 caatgctgtg tttgtcatca gtctcagcgc ccggtgcaat acgtgacatc gtgagccatg
     7441 tcagtaagtg gttaaattcg cagtggtagc atgccttccg tgatttctta ctaaccggag
     7501 aagtaatgga actgctttta ttgagtaact cgacgctgcc gggtaaagcc tggctggaac
     7561 atgcactgcc gctaattgct gaacagttgc agggtcgccg ctcagcggtg tttatccctt
     7621 tcgctggcgt aacgcagacc tgggatgatt acacagagaa aacggctgcg gttctcgctc
     7681 cgctgggtgt ttctgtcacc ggtattcata gcgttgtcga tcccgttgcc gcgattgaaa
     7741 atgcggaaat tgtgattgtc ggcggcggta atactttcca gttgctgaaa cagtgccgcg
     7801 agcgcgggct gctggctcca attactgacg tggttaaacg tggcgcgctg tatattggct
     7861 ggagcgcagg cgctaacctt gcttgcccaa ccattcgtac caccaacgat atgccgattg
     7921 tcgatccgca aggtttcgat gcgctaaatc tgttcccgtt gcaaatcaac ccgcacttca
     7981 ccaacgcgct gccggaaggc cataaaggtg aaacccgtga gcagcgtatt cgcgaactgc
     8041 tggtcgtcgc gccagaactg acgattattg gtctgccgga aggtaactgg atcacagtga
     8101 gtaaaggtca cgctacgctg ggtggcccga acaccactta tgtgtttaag gctggtgaag
     8161 aagcggttcc gctggaagct ggtcaccgtt tttaagtgaa ttgccggatg catgatccgg
     8221 catcttgttc atagtgcctg atgcgacgct tgacgcgtct tatcaggcct acaggattca
     8281 caatattaat actcgtcccg tccttcatct tcatccggct gttccagcac actgtaagcc
     8341 accgaacaaa acagcgagtt caggcgctgc atatctccca gtaagcctaa atgtagcgaa
     8401 ctggtttcaa tgctctgcac gttttgctga tgcaggcgat cgacgtgggc gtgggaatag
     8461 cggcgattaa gaatgcgaaa acgatgtttg ctgcgacgca aacgacgagc gctggtgaca
     8521 tcgccagaga agaacaccga cattgccagc tttaaattac tgagcaattg ctcatagagc
     8581 gcatccagtt ccttcaaccc atcaagcgaa aatgcccgcc gtgctgccag cgatttatcg
     8641 gcaatttcgc tgcccatgcg ctcgacgata tcggaggcct gttcaaggtt gagcgacatt
     8701 tcgatgatct ccgcccagcg gcgcgactct tcctccgcca actcttcttt tggcatccgc
     8761 gccagataca gcttaatggc ggtgtagaga acgttgatat catctgccag cttacgcagc
     8821 tctttctcct gccgtggctc gccgtgcatc actttattca gcccttccat catctgttcc
     8881 atggcgtcgc caatgcgcag ggtttcgcgc gcggcgttcg ccagagcaag cgtgggcgtg
     8941 tccagcgcgc tgacatccag atgtttaggc cgtagctggg tatccagttc cggttcatcg
     9001 cgaataatcg ttttgcaaaa ccgtgccatc gggtcaacaa atggcagcat gaccaggcaa
     9061 cgtacaaggt tgtagaagac gtggaaatag atcaccagtt ccgcttttgg caatgacagc
     9121 ttccccattg tctctgccag caaatggaca aacggcagga taatcaggct acccaccagc
     9181 ttaaacagca gactacccag cgcgacacgg cgggctgcgg cattggcggc actgttgttg
     9241 agcatcgcca gcaggccgga accgaggtta gctccaatca ccagacagag cgccacgggg
     9301 aaggagataa tgcctgcagc ggtcagagtc gcggtcagca gtacagcagc aaggctggag
     9361 tagctgataa tggcgaacat cgcgccaatc agcgcatcca gcagaatatc gccggtcagc
     9421 gaggcaaaga tcacctgaac gccgtttgcc tgggtgatcg gcgttacggc ctgcacaatc
     9481 aactccagcg ccagcaaaat cagcccaaga ccaataccga cgcggcccag ttgcccggcg
     9541 cgtgactgtt tgcgtccgag gaaaaaaatc acgccgataa aaataagtaa cggtgacagc
     9601 caggataagt cgaaggtgag aatacgcgcc attagcgccg tcccgacatc tgcccccagc
     9661 acaatgacca gagccggtgc gagggctacc agatcctgag cgacgaacga ggtcaccagc
     9721 atggtggtgg cattactgct ctgtaccagt gcggtaacgc cgatccccgc gcaaaaggcg
     9781 agcggctttt tttcgacgct ccggctaagg acagtacgca aacgcgcgcc gaagacgcgc
     9841 attacgccgg ttcgaacaat atgagtcccc cagaccagca gggcgacggc agaaagcagg
     9901 tgaagcagcg ttaacacgag ttcagattct ccttatcgtt atatgttcct gaagcaattg
     9961 aatgaccaaa ggaccggaaa cacgctgact ggcatcttta ctgtgccttt tgacattcgt
    10021 ttttccggct aagattattc cagcctgttt ccagtataag tgtttcatat taataaagag
    10081 acagggcact cattgagcgc cctgtttgtt gtaaggaaaa gtgacagatt tgtgaatcag
    10141 tccgcgtcat accccagatt cggtgccagc cagcgctcaa catcggagac gctcatacct
    10201 ttacggcggg catggtcttc aacctgatcg cgctgaattt gcgccaccgc gtagtacttg
    10261 ctgtccggat gactgaaata ccaacccgaa accgatgcac caggccacat cgcgaaagat
    10321 tctgtgagtt tcatgccagt atgtttttcc acttccagca gctcccagat ggtggctttt
    10381 tccgtatgtt ccgggcaggc cggatagccc ggtgccggac ggatgccctg gtagttttcg
    10441 cggatcagtt cttcgttgct gaggttctcg ttcggcgcat agccccagta gactttacgc
    10501 acacgctcat ggagatactc cgcaaaggct tcggctaaac ggtcggcaag cgctttcacc
    10561 atgattttgt tgtaatcatc gtgctgcgct tcaaaggcat cagccagtgc gtcctcttcc
    10621 agcccgccag tcacggcaaa tgcgccgatg taatctgctt taccagaaag cttcggcgca
    10681 acgaagtcag cgagacagta gttagcgaat cccgtttttt cggtctgttg acgcagatgg
    10741 tggctgacgt tgatcacatg ggtgcgcgtt tcgtcacggt agatttcaat gtcatcgccc
    10801 acacggtttg ccgggaacag gcccaccacg ccacgcggat tcagcgtttt ctcggcgctt
    10861 aatttatcca gcatgtcgtt ggcgtcttta aacagccgct gcgcctcaac gcccaccact
    10921 tcatcttcca gaatgcgagg atacttcccg gccagcgacc aggtcataaa gaacggtgtc
    10981 cagtcgatgt aattacgcag cgtttcgatg ctggcttcca cttcctgcac gccgagacgg
    11041 tgcgccaccg gcggcgtgta agcctgccag tcaaatgcga aatcgttatc gcgcgccgct
    11101 tccagcgtga ccggtggtgt gcgtggtttc ttgcgcccgt gctgaatacg cacggtttcg
    11161 tactccttgc aggtacgagc gacaaaatca tcacgctgag tatcggaaag cagcgccgct
    11221 accacaccaa cggtgcgcga ggcattctgc acatacaccg tagggccgct gtagttctgc
    11281 tcgattttca ccgccgtgtg cgcttttgag gtggtcgcgc cgccaatcag taacggaatg
    11341 gcgaagccct gacgctccat ctctttcgcc acgttaacca tctcgtccag cgacggcgtg
    11401 ataagccccg aaaggccaat caaatcagca ttcacttctt tggcgatacg gagaattttt
    11461 tccgcaggca ccataacgcc gagatcgaca atttcgtagt tgttacattg cagcaccaca
    11521 ccaacgatat ttttaccgat gtcgtggaca tcgcctttca cggtggcgat caccatcttg
    11581 ccgttggttt taccctgctc tttgctggct tcaataaacg gttcgagata cgccactgcc
    11641 tgtttcatga cgcgcgccga tttgaccacc tgtggcagga acattttgcc ttcgccaaac
    11701 agatcgccga ccacgttcat gccgtccatc aacgggcctt caatcacttc aatcgggcgc
    11761 gtagcctgct ggcgggcttc ttcagtatcc tgctcgataa actcggttat gcctttgacc
    11821 agcgagtatt ccagacgttt attcacgtcc cacgagcgcc actccgcttg ctgggcgtta
    11881 gcagtgtcgt ccgctttact gccgcgatat ttctcggcaa gctccagtaa acgctcagtg
    11941 ccatcgtcac gacgattaag aatcacatct tccaccgcgt cgcgcagttc agcgggtagg
    12001 tcgtcgtaaa tcgccagttg cccggcgttg acgatcccca tatccatgcc attgcgaata
    12061 gcgtagtaga ggaacactgc gtgaatggct tcgcgcaccg gatcgttgcc acggaatgag
    12121 aaagaaacgt tagatacgcc gccggaaatc agcgcgtgcg gcagttcgcg tttgatgtct
    12181 tcacacgcgc cgataaagtc ctgcgcgtag ttgttgtgct cttcaatacc ggtggcgacc
    12241 gcgaagatgt tcgggtcgaa gatgatatct tctggcggga agccaacctc ttcggtgagg
    12301 attttgtacg cccgacggca aatctcgatt ttccgtgcgc gagtatcggc ctgtccctgt
    12361 tcgtcaaagg ccatcaccac cactgccgca ccgtagcgac gcaacaattt cgcgtgatgg
    12421 ataaaggcgt cgacgccctc tttcatcgag atagagttaa caatgccttt gccctggata
    12481 cacttcagac ctttttcaat gacatcccat tttgaggagt cgatcataat cggcacgcga
    12541 gcgatatccg gttcaccggc aatcagattg agaaaacgca ccatcgccgc ttcggcatcg
    12601 agcatccctt catccatgtt gatatcgata atctgcgcgc cgttttccac ctgctgacgc
    12661 gccacatcca gcgcctcgct gtatttctct tctttgatca ggcgcttgaa cttagcagag
    12721 ccggtgacgt tggtgcgttc acccacgttc acaaacaggc tatcgtcgcc aatgttcagc
    12781 ggctccaggc cagacaaacg gcaggctacg ggaatttccg gcagtttgcg cggcgctaat
    12841 ccttctactg cacgactcat cgctgcaata tgttgcggcg tggtcccgca gcaaccaccg
    12901 acgatattga gaaagcccgc ttgcgcccat tcacgtatct gttttgccat cgtgtcggcg
    12961 tcgagatcgt attcaccaaa ggcgttaggc agcccggcgt tcgggtgggc ggtgacgtag
    13021 cattccgcaa tccgtgatag ttcctgcacg tactggcgca gttcatcggg ccccagcgca
    13081 cagttcaggc caaaggtcag cgcttcggcg tggcgcaatg agttgtaaaa tgcttcggtg
    13141 gtctgcccgg agagcgtgcg cccggaggcg tcggtgatgg tgccggagat cataatcggc
    13201 agctcaacgc ccagcgcttc aaactccgtt ttcaccgcaa agaccgccgc tttggcgtta
    13261 agggtgtcga aaacggtttc aatcaggatc agatccgcac caccttccac cagcgctttg
    13321 gtggattcgc gataggccgc cgccagctgg tcaaaagtga tgttgcggaa tgccggatcg
    13381 ttgacgtccg gagaaataga cgccgtgcgg ttggttgggc caagaacacc ggcaacgtag
    13441 cgcggtttct ccggcgtgcg cgcggtccac tcgtcagcac aggcgcgcgc cagtttcgcc
    13501 gccgcaaagt tgatttccgc cgacagggat tccatctggt aatccgccat cgcaatggtc
    13561 gtggagttga aggtgttggt ttcgatgata tccgcgcccg cttcaaagta ggcgttgtgg
    13621 atagcggcga tcacttccgg tttactgagt accagcaggt cgttgttgcc tttgaggtcg
    13681 catggccagt cggcaaagcg ttcaccacga aaatcggctt cgttcagtcg atagctctgg
    13741 atcatggtgc ccataccgcc gtccaacacc agaatacgtt catttaactg cgcacgcagt
    13801 tgttccactt tgctgctcac acttgctccc gacacgctta acccagattt aataaaaatt
    13861 caacaaacca tactggaata aacgcatctg tggtaaaagc gaccaccacg caacatgaga
    13921 tttgttcaac attaactcat cggatcagtt cagcaactat tgcattagct aacaataaaa
    13981 atgaaaataa tttccacgat acagaaaaag gaggctgtca tggtcgcacc cattcccgcg
    14041 aaacgcggca gaaaacccgc cgttgccacc gcaccagcga ctggacaggt tcagtcttta
    14101 acgcgtggcc tgaaattact ggagtggatt gccgaatcca atggcagcgt ggcactcaca
    14161 gagctggcgc aacaagccgg gttacccaat tccacgaccc accgcctgct aaccacgatg
    14221 caacagcagg gtttcgtgcg tcaggtcggc gaactgggac actgggcaat cggcgcacat
    14281 gcctttatgg tcggcagcag ctttctccag agccgtaatt tgttagcaat tgttcaccct
    14341 atcctgcgca atctaatgga agagtctggc gaaacggtca atatggcggt gcttgatcaa
    14401 agcgatcacg aagcgattat tatcgaccag gtacagtgta cccacctgat gcgaatgtcc
    14461 gcgcctatcg gcggtaaatt gccgatgcac gcttccggtg cgggtaaagc ctttttagcc
    14521 caactgagcg aagaacaggt gacgaagctg ctgcaccgca aagggttaca tgcctatacc
    14581 cacgcaactc tggtgtctcc tgtgcattta aaagaagatc tcgcccaaac gcgcaaacgg
    14641 ggttattcat ttgacgatga ggaacatgcg ctggggctac gttgccttgc agcgtgtatt
    14701 ttcgatgagc accgcgaacc gtttgccgca atttctattt ccggaccgat ttcacgtatt
    14761 accgatgatc gcgtgaccga gtttggcgcg atggtgatta aagcggcgaa ggaagtgacg
    14821 ctggcgtacg gtggaatgcg ctgacttttt ctggcgggca gaggcaatat tctgcccatc
    14881 atacctgagt ggcaatagaa taagggtgtc tgttaatcgc attgacgcca aaataactta
    14941 atgtcataca cttcacacac ttttattaac tcagcattat ttttaaacat caaaccactt
    15001 aattattaac aaaatcgcaa gatctcattc caccgaaact tttacggctg cattatgaga
    15061 ttgagcgatt tatgatattt ttttagttgt aatcatatta aattttttca ctgttctgat
    15121 agttaaaatt caagacatca ataaacaatg agatatttaa atgattactc gtattcctcg
    15181 tagttctttc tctgcaaata ttaataatac agcccagaca aatgaacacc aaaccctgag
    15241 tgaattgttt tataaagaac tcgaggataa attttctggc aaggagctgg cgacgcctct
    15301 attaaaaagc ttctcagaga actgtagaca aaatgttcgt catatcttta gcaacaagga
    15361 ttttgtcatt aaattttcca cgtccgtctt acaagctgat aagaaagaaa ttacgatgat
    15421 taataaaaac gaaaacacga cactcactca aaccattgct acaatatttg aagaatacct
    15481 aatggaaatt ttacctcaac gctcagacac tcttgataaa caagaattaa accaaaaatc
    15541 agatagaaaa gaaaaagaat tcccaagaat taaacttaat ggtcaatgtt atttcccggg
    15601 gcgccccaaa accgtatagt ataccgacac attgctgcac aatatattaa tgatatttat
    15661 cagaatgttg attacaaacc ccatcaagat gattactctt cagctgaaaa atttctcact
    15721 cacttcaaca aaaaatgcaa aaaccagact ttggcgttga tttccagccg tcctgagggg
    15781 cgttgcgttg ctgcctgcgg tgatttcggg ctagttatga aagcatattt tgacaagatg
    15841 gaatcaaatg gcatcagtgt tatggcagcc atattactgg tggataacca tgctttgacg
    15901 gtccggctaa gaataaagaa cacaactgaa ggatgtaccc attacgtggt ttcggtttat
    15961 gatcctaatg taactaacga taaaataaga attatgagcg aaagcaaaga ggatattaaa
    16021 cactattctc tgatggattt tatgaatgta gattatagcc tcctgaaatg gtcaaatgat
    16081 catgttatta accaatctgt tgcaataatt ccagcacttc cgaaagaaca gctattgatg
    16141 ttaaaaggaa ctgtggatga aataacccct ccattatcac cagcaacgat gaatttgcta
    16201 atggcaattg gtcagaatca ccaacttacg caactgatga ttcagctcca gaaaatgcca
    16261 gaactacata gaacagaaat gttgactgcc tataatagta ttaacctccc cggtttatat
    16321 ttggctataa attatggcaa tgcggatatc gttgagacta ttttcaattc attgtcagaa
    16381 acaggatatg aagggttact ctcgaaaaaa aatctcatgc atattctgga ggcaaaagat
    16441 aaaaatggtt tttctggatt atttttagcg atatcacgta aggataaaaa tgttgtaacc
    16501 tcgattctga acgtcttacc taaactggcc gcaacacatc atttagataa tgaacaggtg
    16561 tataaattcc tgagtgccaa aaatagaacg tccagccatg ttttatacca tgttatggcg
    16621 aatggtgatg ccgacatgct gaaaattttt ttggtcgcgt tacctttgtt aattcgcaca
    16681 tgtcatttga ctaaagaaca ggtactcgat ctcctgaagg caaaggattt ttatggttgc
    16741 ccaagactat acctggcgat gcaaaatgga catagcgata tcgtgaaagt tattctcgaa
    16801 gcattgccct gtctggccca ggaaattaac atttcagctt ccgatattgt agatcttctg
    16861 accgctaaaa gtcttgcgcg cgacacgggt ttgtttatgg ccatgcagcg cggacatatg
    16921 aacgttatta atactatttt taacgcatta cccactcttt ttaatacgtt taaattcgat
    16981 aaaaaaatat gaagcccctc ctcctggcaa ataattctaa tgaatatccc ggtttgtttt
    17041 cagcgataca gcataaacaa caaaacgttg tagagacggt ttatcttgct ttatctgacc
    17101 atgcacgcct gtttggattt accgctgaag atattatgga tttttggcaa cacaaagcgc
    17161 cacaaaaata ctctgccttt gagttggctt ttgaatttgg tcaccgggtt attgctgaat
    17221 taatccttaa tacattaaat aagatggctg aaagctttgg ctttacggat aaccctcgat
    17281 acattgcgga gaaaaattat atggaagctt tactcaaaaa agcatctccc cataccgtac
    17341 gctaaatctt tgcctgcgcc gatacgcata aacatcttcc acatgccctt cacgtatgcg
    17401 gttttgtagt gcgcgccagt aatcagcgcg gaacaggtcg gcgtgcatct cttcaaacag
    17461 cggaccaata cgcgggtcgg cgcatagcca gtggcgaaac tcttccggga aaacatcgcc
    17521 cggcgagacg ctgtaccacg gttcgctggc aagttcgtct tccggatagc gcggcggcgg
    17581 gatgtcgcgg aaattcactt ccgtcatgta gcaaatttca tcgtaatcat aaaaaaccac
    17641 acgcccgtga cgggtgacac cgaagttttt aaacagcatg tcgccaggga aaatgttagc
    17701 agcggcaagc tggcgaatag cgttaccgta ttcttcaatg gcgtcgcgca actgctgacc
    17761 ttccacttgt tccagccaga tattgagcgg caccatccgc cgctcgatta tagagatggc
    17821 gaattacaat ctgctcacca agatcggtga ttttttccgc tgcttcctgg agcagtaatt
    17881 ccattaatac cggggaaata tgccgcttct ccagcacaaa gttttcaaac tcctgggtgt
    17941 ccgccattcg gcccacacgg tcgtgctctt tcaccagttg atagcaggca cgaacgtgag
    18001 cggcagacat ctctttctgc ggcgcgaact tgtctttgat gactttgaat acccgatcaa
    18061 agcccggcag cgtaaacacc aacatcacca ttccacgaat acccggcgct tcaatgaact
    18121 gctcattaca gccctgtaga taaacgagat attcgcggta gctttcggtt ttggcgtgct
    18181 tctggcagcc gatagccata tacaattcag cagtggtttt acctggcaga atttcccgta
    18241 gccactcgac cagcgctgcg ggcagcggtg cataaaccat aaaataagaa cgcgcaaagc
    18301 caaaaacaat gctcgcttcg gcggtcgtcg tcaggcaggt atcaataaat aactcgccgt
    18361 cgtccgtctg gtggatcggc agtaagaagg gcaacgtgcc ggaaggcgtg atcagtttgc
    18421 ccaccagcca ggcggcttta ttgcggtaaa acagttcgtt cgccacctgt aaatgacttt
    18481 ccgcgaggtt gtctgtcccc agcgtttccg tcagatggcg aatgatgtaa tgaatgtcac
    18541 ggcttttatt ctgccagcgc aggcgcagcg gcaggtcgct gataacgcgc atcagtagag
    18601 attcccagcc gtgatcgggg tgaaagtctt tcgccagcgg gcggggaatg gtacgaaagc
    18661 ggcgctctgg ctgagagcta aagataaaaa gccgctcggg agtaagcgag cggtggtcaa
    18721 ataaccgaca gtacacggag ttaaaaaagc tctccgcaat ctcgaagcgc gggtaatccg
    18781 gcaacagccg cgtgtaatgc tctttaacgc gcagtaaaaa tgccgcgtcc gtactttggc
    18841 cgttagtaat gcagcgcagt tgctccacga ccagaccaac gtgatgatcg taaagatgga
    18901 tacggttttt catcgcctgc tggacagcat gccagtcggc ctgttcgaaa cgctgctgcg
    18961 ccccggaagt cacttcgagg aatcgaccat actgagcgtc gaagccttgc aaaatggttt
    19021 gagcaatcag taattccagg ccacgcggca tttagcgccc tcatcaggag cagagaattg
    19081 ccggatgcgg cgtaaacgcc ttatccagcc tacgttcggc aacggttgta ggcatgataa
    19141 gacgcgcaag cgtcgcatca ggcatcgtgc accgattgcc ggatgcggcg taaatgcctt
    19201 atccggccta cagtcagcaa cgtttgtagg ttgcttagaa ctgcgattct tcagtggagc
    19261 cagtcagcgc ggtgactgaa gacgtgccgc cctgaataat agtcgtcact ttatcgaagt
    19321 aacctgtacc cacttcctgc tggtgagata cgaaggtata gccatctttc gcggcggcaa
    19381 attccggctg ctgcactttc tcaacatagt gcttcatacc ttcgccctgc gcataagcgt
    19441 ttgccaggtc gaacatgtta aaccacatgc tgtggatacc tgctagggtg atgaactgga
    19501 acttgtagcc catatccgac agctgctgct ggaagctggc aatagttttg tcgtcgaggt
    19561 ttttctgcca gttgaacgac ggcgagcagt tataagccag cagtttgccc ggatatttcg
    19621 cgtggatagc ttgtgcaaag cgacgcgcca gttccagatc cggcgtggag gtttcacacc
    19681 agaccaggtc agcatatggc gcatacgcca ggccacggct gatcgcttgc tcaatgcccg
    19741 catgagtacg gaagaagcct tcactggtac gctcgccggt aataaattcg ctgtcatacg
    19801 ggtcgcaatc tgaggtgatc agatccgccg catcagcatc ggtacgtgca accagcaggg
    19861 ttggaacgcc cgtcacgtca gctgccagac gcgccgcgac cagtttctga atagcttcct
    19921 gagttggcac taaaactttg ccgcccatgt gaccgcattt cttcactgac gccagctgat
    19981 cttcgaagtg aactgccgca gcaccggctt caatcatcgc tttcatcagt tcaaaggcat
    20041 tcaggacacc gccaaaaccg gcttccgcat cggcaacgat cggcaggaag taatcgacat
    20101 agcgcggatc gcccggctca atgcccacgg accattggat ctgatcggca cgacggaagg
    20161 tgttgttgat ccgctccacc acagctggca ccgagtttgc cggatagagc gactgatccg
    20221 gatacatgct ggccgccagg ttagcgtccg ccgctacctg ccatcccgac agatagactg
    20281 cttcaatacc cgctttcgcc tgttgcagcg cctgaccgcc agtcagtgca ccgaggttgt
    20341 tgatgtagcc ttttttcgac tcaccgtgca gcagacgcca cattttcgct gcgcccagtt
    20401 gcgccagcgt gcattcagga ttgactgaac cgcgtaattt caccacatct tccgcactgt
    20461 atgggcgagt aatgccttcc caacgcggtt gagtccactc tttctgtaat tcttcaattt
    20521 gttgtgtacg ggttttcatg tgcagatgct ccatagttat gtggtggttt acgctaacag
    20581 gcggtagcct ggcagggtca ggaaatcaat taactcatcg gaagtggtga tctgttccat
    20641 caagcgtgcg gcatcgtcaa aacggccctg ggagaaacgt tcttcgccca gttcgctggc
    20701 aatgactttc atctcttcgc ccagcatctg gcggaacaag gctttggtca ccggtttgcc
    20761 attgctcaac gttttttgat gatggatcca ctgccagatc gaggtacggg aaatttcagc
    20821 cgtcgccgca tcttccatca ggccataaat cggcacgcag ccgttgccgg agatccacgc
    20881 ttcgatgtac tgcacagcca cgcgaatgtt agcgcgcata ccttcttcgg tgcgttcacc
    20941 atcacaaggt gccagcagct gatcggcagt aatcggcgcg tcttgttcgc gcaatacttc
    21001 aagctgattt ttacgggagc cgagaatgtc gttgaatacc gccattgccg tgtccgcaag
    21061 gcctgggtga gcgatccatg tgccatcgtg accgttattg gcttccagcg ctttatccgc
    21121 ttttactttg ttgagcacct ggttattacg ctcttcatct ttgctcggaa taaacgcggc
    21181 catgccgccc atcgcaaaag caccgcgttt atggcaggtt ttaatcagca ggcgtgagta
    21241 agcattcagg aatggtttat ccatcgtcac ggcctgtctg tctggcagga cacgatcggg
    21301 atagtttttc aacgttttga tatagctgaa gatgtaatcc cagcgaccgc agttcagacc
    21361 aacaatatgg tcgcgcagcg cgtgaaggat ttcgtccatc tggaacacgg cgggcagcgt
    21421 ttcaatcagc aacgtcgcct tgatggtgcc gcgcggcaga ttaaagcgat cttctgcata
    21481 gctgaagact tcgctccacc aggccgcttc ctgctaggac tgggttttcg gcagatagaa
    21541 atagggaccg ctgccctttg ccaacagtgc ctgatagttg tggaagaaat agagcgcaaa
    21601 atcaaacagg ctaccgggga ttgcctcacc acgccaggtg acatgttttt ccggcaagtg
    21661 cagaccgcgt acccgacaaa tcaaaaccgc tggattgggc ttgagctgat aaattttgcc
    21721 tgcttcattg gtatagctga tggtaccgtt aaccgcatcg cgcaggttaa tttgcccgtc
    21781 gatcactttg ttccagtctg gtgccagtga atcttcgaaa tcggccataa agactttcac
    21841 attggcgttc agtgcgttga tcaccatctt gcgctctacc gggccagtta tctctacgcg
    21901 gcggtcttct aagtccgcag gaatcccgcg aattttccaa tcagcatcgc gaatggaagc
    21961 cgtttccgaa ataaaatcag gcaacgttcc gttatcaata tcttgctgct gctgaatgcg
    22021 cgctgccaga agtttattgc gttgtggcgt aaaatgcgtc accagctcag tcagaaattc
    22081 taccgcttcg gcagtaagaa tttgcttctc ctgctcgcca tacggccttg tgaaagccag
    22141 ttcatcggtt gttgttgcct gttcagtcat cgtgcagctc ctcgtcatgg atccgaaaac
    22201 ttcccctctg tgaacgaagg aacgttgtgc gcttttcgtt cagcacaact aagactactc
    22261 atttaaaatg caaaatcaaa aacaatttcc attttaatta aaaatacttg ataacttatt
    22321 gataacaata tgaataaaat ttattcactt gctgaggtgc gtttcggaaa taaaaaaggc
    22381 acccgaaggt gcctgaggta aggtgctgaa tcgcttaacg atcgactatc acagaagatt
    22441 aatccagcgt tggattcatg tgccgtagat cgtatggcgt gatctgatag acgtaatagt
    22501 tgagccagtt ggtaaacagc aaattaccgt gactacgcca gctcgctcgc ggtttgtttt
    22561 gcggatcgtt gtgcgggaaa tagttatacg gtacatccgg gtctagtccg gcttccacat
    22621 cgcgtaaata ttcctgcgcc agcgtttgcg catcatattc gggatggccc gtcacaaagg
    22681 caatgcgctt atctttgctg gcaaacagat atgcatcccc ttcttccgtc tctgccagaa
    22741 tttccagatc ggtgtaatca cgaatcaacg ctgccggaaa gtcagcatag cgcgaatgcg
    22801 gtgccaggaa tgaatcatca aagccacgcg tcagaagcgc atgaggatgg agaatatgat
    22861 gctcgtaaac gccagagagt ttgtcggtgc gagtttgctt aggaatgccg tagaggatat
    22921 tgagcgcggc ctgtaccgcc cagcagacaa acagcgtcga ggtgacgtga tctttcgacc
    22981 actccagcac ctgtttgatc tgcggccagt aagcgacatc attaaactcc accaggccca
    23041 gcggcgcacc ggttacaatc agaccgtcaa agttctgttc ctgaatatct tcaaagttac
    23101 agtagaagtt gttcagatgc tctgcgggcg tgttgcgcga ttcacgggaa tcgatgcgca
    23161 acagctgaat atcgacctgc aaaggtgagt ttgaaagcag gcgcagaaac tgattttcag
    23221 tttcaatctt cttcggcatc aggttaagga tcagaacctt aagtggacga atttcctgac
    23281 cagacgcacg agaagttgtc atcacaaaga cgttttcttc acgcaagaaa ttgacggcgg
    23341 gtagctcgtc cggcacacga atcggcataa cctgattacc tcactacata cgcttatacg
    23401 tttagacatc cagatagctg aagataacca gaaaatttgc caatgtcgag cctgcatgtt
    23461 gaaggtgaga aagtttcaag gaaaaggcta cgttagaata taagaatgac gaaaaggaga
    23521 gaaaatggtt attagtattc gccgctcacg gcatgaggaa ggggaggaac tcgttgcgat
    23581 ttggtgtcga tctgtcgatg ccacccacga ttttttatca gcagagtatc gggccgagct
    23641 ggaagagctg gtgcgttcct ttctgccgga agcaccgttg tgggtcgcgg ttaatgaacg
    23701 ggagcagccg gttgggttta tgttgttaag tgggcagcat atggatgcgc tgtttatcga
    23761 tcctgatgtg tgcggctgcg gcgtgggtcg gatgctggtg gagcatgcgc tctcgatggc
    23821 accggaactg acaaccaacg ttaatgagca aaatgagcag gcggttgggt tctataagaa
    23881 ggtgggtttt aaggttacgg gacgctctga ggtggacgat ttggggaaac cgtatccgtt
    23941 gctgaatctg gcgtatgtgg gggaataagc cgcctctgcc gtgcctgccc aagctggaga
    24001 agacg
//
LOCUS       DAJTUP010000060        22879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTUP010000060 DAJTUP010000000
VERSION     DAJTUP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.060"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1850
                     /gene="thrS"
                     /locus_tag="OO468_002434"
     CDS             <1..1850
                     /gene="thrS"
                     /locus_tag="OO468_002434"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR6259514.1"
                     /translation="GPGLAKACIAGRVNGELVDACDLIENDAQLSIITAKDEEGLEII
                     RHSCAHLLGHAIKQLWPHTKMAIGPVIDNGFYYDVDLDRTLTQEDVEALEKRMHELAE
                     KNYDVIKKKVSWHEARETFANRGESYKVSILDENIAHDDKPGLYFHEEYVDMCRGPHV
                     PNMRFCHHFKLMKTAGAYWRGDSNNKMLQRIYGTAWADKKALNAYLQRLEEAAKRDHR
                     KIGKQLDLYHMQEEAPGMVFWHNDGWTIFRELEVFVRSKLKEYQYQEVKGPFMMDRVL
                     WEKTGHWDNYKDAMFTTSSENREYCIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGSC
                     HRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQIRDEVNGCIRLVYDMYSTFGFEKIVVK
                     LSTRPEKRIGSDEMWDRAEADLAVALEENNIPFEYQLGEGAFYGPKIEFTLYDCLDRA
                     WQCGTVQLDFSLPSRLSASYVGEDNERKVPVMIHRAILGSMERFIGILTEEFAGFFPT
                     WLAPVQVVIMNITDSQSEYVNELTQKLSNAGIRVKADLRNEKIGFKIREHTLRRVPYM
                     LVCGDKEVESGKVAVRTRRGKDLGSMDVNEVIEKLQQEIRSRSLKQLEE"
     gene            1854..2396
                     /gene="infC"
                     /locus_tag="OO468_002435"
     CDS             1854..2396
                     /gene="infC"
                     /locus_tag="OO468_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR6259515.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            2493..2690
                     /gene="rpmI"
                     /locus_tag="OO468_002436"
     CDS             2493..2690
                     /gene="rpmI"
                     /locus_tag="OO468_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR6259516.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            2743..3099
                     /gene="rplT"
                     /locus_tag="OO468_002437"
     CDS             2743..3099
                     /gene="rplT"
                     /locus_tag="OO468_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR6259517.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3216..3340
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3222..3266
                     /gene="pheM"
                     /locus_tag="OO468_002438"
     CDS             3222..3266
                     /gene="pheM"
                     /locus_tag="OO468_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR6259518.1"
                     /translation="MNAAIFRFFFYFST"
     gene            3549..4532
                     /gene="pheS"
                     /locus_tag="OO468_002439"
     CDS             3549..4532
                     /gene="pheS"
                     /locus_tag="OO468_002439"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR6259519.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            4547..6934
                     /gene="pheT"
                     /locus_tag="OO468_002440"
     CDS             4547..6934
                     /gene="pheT"
                     /locus_tag="OO468_002440"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR6259520.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            6939..7238
                     /gene="ihfA"
                     /locus_tag="OO468_002441"
     CDS             6939..7238
                     /gene="ihfA"
                     /locus_tag="OO468_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR6259521.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7339..8319
                     /gene="btuC"
                     /locus_tag="OO468_002442"
     CDS             7339..8319
                     /gene="btuC"
                     /locus_tag="OO468_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR6259522.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8382..8933
                     /gene="btuE"
                     /locus_tag="OO468_002443"
     CDS             8382..8933
                     /gene="btuE"
                     /locus_tag="OO468_002443"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR6259523.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            8933..9682
                     /gene="btuD"
                     /locus_tag="OO468_002444"
     CDS             8933..9682
                     /gene="btuD"
                     /locus_tag="OO468_002444"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR6259524.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            9760..10224
                     /locus_tag="OO468_002445"
     CDS             9760..10224
                     /locus_tag="OO468_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6259525.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            10471..11184
                     /gene="ydiV"
                     /locus_tag="OO468_002446"
     CDS             10471..11184
                     /gene="ydiV"
                     /locus_tag="OO468_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR6259526.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11248..12684
                     /locus_tag="OO468_002447"
     CDS             11248..12684
                     /locus_tag="OO468_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR6259527.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(12688..12879)
                     /gene="hemP"
                     /locus_tag="OO468_002448"
     CDS             complement(12688..12879)
                     /gene="hemP"
                     /locus_tag="OO468_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR6259528.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13011..14057)
                     /gene="aroH"
                     /locus_tag="OO468_002449"
     CDS             complement(13011..14057)
                     /gene="aroH"
                     /locus_tag="OO468_002449"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR6259529.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14214..15047)
                     /gene="ppsR"
                     /locus_tag="OO468_002450"
     CDS             complement(14214..15047)
                     /gene="ppsR"
                     /locus_tag="OO468_002450"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR6259530.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15379..17757
                     /gene="ppsA"
                     /locus_tag="OO468_002451"
     CDS             15379..17757
                     /gene="ppsA"
                     /locus_tag="OO468_002451"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR6259531.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(17814..19460)
                     /gene="fadK"
                     /locus_tag="OO468_002452"
     CDS             complement(17814..19460)
                     /gene="fadK"
                     /locus_tag="OO468_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR6259532.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(19517..19810)
                     /locus_tag="OO468_002453"
     CDS             complement(19517..19810)
                     /locus_tag="OO468_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR6259533.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(19807..21096)
                     /locus_tag="OO468_002454"
     CDS             complement(19807..21096)
                     /locus_tag="OO468_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR6259534.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21152..22090)
                     /locus_tag="OO468_002455"
     CDS             complement(21152..22090)
                     /locus_tag="OO468_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR6259535.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22110..22874)
                     /locus_tag="OO468_002456"
     CDS             complement(22110..22874)
                     /locus_tag="OO468_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR6259536.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5611 a         5625 c         6068 g         5575 t
ORIGIN      
        1 ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa ctggttgatg
       61 cttgcgatct gattgaaaac gacgcacaac tgtcgatcat taccgccaaa gacgaagaag
      121 gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcatgcgatt aaacaacttt
      181 ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat tacgacgttg
      241 atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg atgcatgagc
      301 ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa gcgcgtgaaa
      361 ctttcgccaa ccgtggggag agctacaaag tttccattct tgacgaaaac atcgcccatg
      421 atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc ggtccgcacg
      481 taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg gcttactggc
      541 gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg gcagacaaaa
      601 aagcacttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac caccgtaaaa
      661 tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg gtattctggc
      721 acaacgacgg ctggaccatc ttccgtgaac tggaagtgtt tgttcgttct aaactgaaag
      781 agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg tgggaaaaaa
      841 ccggtcactg ggacaactac aaagatgcaa tgttcaccac atcttctgag aaccgtgaat
      901 actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag gggctgaagt
      961 cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt aacgagccgt
     1021 caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac gcgcatatct
     1081 tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta gtctatgata
     1141 tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt cctgaaaaac
     1201 gtattggcag cgacgaaatg tgggatcgtg ctgaggcgga cctggcggtt gcgctggaag
     1261 aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt ccgaaaattg
     1321 aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta cagctggact
     1381 tctccttgcc gtctcgtctg agcgcctcct atgtgggcga agacaacgag cgtaaggtac
     1441 cggtaatgat tcaccgcgca attctggggt cgatggagcg tttcatcggt atcctgaccg
     1501 aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt atcatgaata
     1561 ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca aatgcgggca
     1621 ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc gagcacactt
     1681 tgcgtcgcgt cccatatatg ctggtctgtg gtgataaaga ggtggaatca ggcaaagttg
     1741 ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa gtgatcgaga
     1801 agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa ggtattaaag
     1861 gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt cgcgcccagg
     1921 aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg agagaagctc
     1981 tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac gccgagccgc
     2041 cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag tcttctaagg
     2101 aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt cctagtacag
     2161 atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa gagggtgata
     2221 aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa atcggtatgg
     2281 aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtagtcgaa tccttcccaa
     2341 cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa cagtaaggcc
     2401 ttcaagtagc actatctgtg gagcctttgg gcttcacagg cattgttcgc ctttgtttcg
     2461 tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc gtacgcggtg
     2521 ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac gctaacctgc
     2581 gtcacattct gactaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg aaagccatgg
     2641 tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa gccgttaacg
     2701 tttttaactt tttaattaga atatagatac aggagagcac atatggctcg cgtaaaacgt
     2761 ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg ctactacggt
     2821 gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg tcagtatgct
     2881 taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc gcgtatcaac
     2941 gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct gaaaaaagcc
     3001 tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa agtagcgttc
     3061 accactctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa gagggagcta
     3121 gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg tagccttttc
     3181 aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct gctattttcc
     3241 gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa gagaaacgga
     3301 aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt tgaaaaataa
     3361 aaagccagtc tatcaggcgt aattgtcgca gccagtctgg acacggccag tgcggagaaa
     3421 ccggagcgta cttaagtacg tgaggatttc gagcactgcc cgggaccaaa atggcaagta
     3481 aaatagcctg atgggatagg ctctaagtcc aacgaaccag tgtcaccact gacacaatga
     3541 ggaaaaccat gtcacatctc gcagaactgg ttgccagtgc gaaggcggcc attagccagg
     3601 cgtcagatgt tgccgcgtta gataatgtgc gcgtcgaata tttgggtaaa aaagggcact
     3661 taacccttca gatgacgacc ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg
     3721 cggttatcaa cgaagcgaaa gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac
     3781 tggaaagcgc tgcactgaat gcgcgtctgg cggcggaaac gattgatgtc tctctgccag
     3841 gtcgtcgcat tgaaaacggc ggtctgcatc cggttacccg taccatcgac cgtatcgaaa
     3901 gtttcttcgg tgagcttggc tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc
     3961 ataacttcga tgctctgaac attcctggtc accacccggc gcgcgctgac cacgacactt
     4021 tctggtttga cgctacccgc ctgctgcgta cccagacctc tggcgtacag atccgcacca
     4081 tgaaagccca gcagccaccg attcgtatca tcgcgcctgg ccgtgtttat cgtaacgact
     4141 acgaccagac tcacacgccg atgttccatc agatggaagg tctgattgtt gataccaaca
     4201 tcagctttac caacctgaaa ggcacgctgc acgacttcct gcgtaacttc tttgaggaag
     4261 atttgcagat tcgcttccgt ccttcctact tcccgtttac cgagccttct gcagaagttg
     4321 acgtcatggg taaaaacggt aaatggctgg aagtgctggg ctgcgggatg gtgcatccga
     4381 acgtgctgcg taacgttagc atcgacccgg aagtttactc tggtttcgcc ttcgggatgg
     4441 ggatggagcg tctgactatg ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa
     4501 acgatctgcg tttcctcaaa cagtttaaat aaggcaggaa tagattatga aattcagtga
     4561 actgtggtta cgcgaatggg tgaacccggc gatcgatagc gatacgctgg cgaatcaaat
     4621 cactatggcg ggcctggaag ttgacggtgt agaaccggtt gccggtagct tccacggcgt
     4681 ggtcgttggt gaagtggttg agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac
     4741 aaaagtgaat gtcggcggcg atcgcctgct ggacatcgtc tgcggtgcgc caaactgccg
     4801 tcagggcctg cgtgtggcgg tagctaccat tggtgctgtt ctgccgggtg atttcaaaat
     4861 taaagcggcg aaactgcgtg gcgaaccgtc tgaagggatg ctgtgctcct tttctgagct
     4921 gggaatttct gacgatcaca gtggcattat cgaactgcct gcggatgcgc cgattggcac
     4981 cgacatccgc gaatacctga aactcgatga taacaccatc gaaatcagcg tgacaccaaa
     5041 ccgtgccgac tgcttaggta tcattggtgt tgcgcgtgac gttgccgtgc tgaatcagct
     5101 gccgctggtt gaaccggaaa tcgttccggt tggtgcgact atcgacgaca cgttgccgat
     5161 tacagtcgaa gcgccggaag cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa
     5221 cgttaaagcg ccaactccgc tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc
     5281 tatcgatgca gttgttgacg tcaccaacta tgtactgctc gaactgggcc agccgatgca
     5341 cgctttcgat aaagatcgca ttgaaggcgg cattgtggtg cggatggcaa aagagggcga
     5401 aacgctggtg ctgcttgatg gcactgaagc gaagctgaat gctgacactc tggtcatcgc
     5461 cgaccacaac aaggcgctgg cgatgggcgg catctttggt ggcgaacact ctggcgtgaa
     5521 tgacgaaacg caaaacgtct tgctggaatg cgctttcttc agcccgctgt ctatcaccgg
     5581 tcgtgctcgt cgtcatggcc tgcatactga tgcgtctcac cgttatgagc gtggcgttga
     5641 tccggtactg cagcacaaag cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg
     5701 tggtgaggct ggcccggtaa ttgatatcac caacgaagca acgctgccga agcgtgcaac
     5761 catcactcta cgtcgtagca aactggatcg cctgataggc catcatattg ctgatgagca
     5821 ggtaactgac attctgcgtc gtctcggctg cgaagtgacc gaaggcaaag acgaatggca
     5881 ggcagttgcg ccgagctggc gtttcgatat ggagattgaa gaagatctgg tcgaagaagt
     5941 cgcgcgtgtt tacggctaca acaacatccc ggatgagccg gtacaggcaa gcctgattat
     6001 gggtactcac cgtgaagctg acctgtcgct caagcgcgtg aaaacgctgc tcaacgacaa
     6061 aggctatcag gaagtgatca cctacagctt cgttgatccg aaagtgcagc agatgatcca
     6121 tccaggcgtt gaagccttac tgctggcaag cccgatctct gttgaaatgt cagcaatgcg
     6181 tctttctctg tggaccggcc tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa
     6241 ccgtgtgcgc attttcgaaa gcggtctgcg tttcgtacca gatactcagg caccgttggg
     6301 cattcgtcag gatctgatgt tagccggtgt gatttgcggt aaccgttacg aagagcactg
     6361 gaacctggca aaagagaccg ttgatttcta tgatttgaaa ggcgaccttg aatccgttct
     6421 cgacctgacc ggtaaactga atgaggttga gttccgtgca gaagcgaatc cggcactgca
     6481 tccggggcaa tccgcagcga tttatctgaa aggtgaacgt attggttttg ttggagttgt
     6541 tcatcctgaa ctggaacgta aactggatct taacggtcgc actctggtgt tcgaactgga
     6601 gtggaacaag ctcgcagacc gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc
     6661 gaaccgtcgt gacatcgcgg tggtggtcgc agaaaacgtt cccgcagcgg atattttatc
     6721 cgaatgtaag aaagttggcg taaatcaggt agttggcgta aacttatttg acgtgtaccg
     6781 cggtaagggt gttgcggagg ggtataagag cctcgccata agcctgatcc tgcaagatac
     6841 cagccgtaca ctcgaagaag aggagattgc cgctaccgtc gccaaatgtg tagaggcatt
     6901 aaaagagcga ttccaggcat cattgaggga ttgaacctat ggcgcttaca aaagctgaaa
     6961 tgtcagaata tctgtttgat aagcttgggc ttagcaagcg ggatgccaaa gaactggttg
     7021 aactgttttt cgaagagatc cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg
     7081 gttttggtaa cttcgatctg cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg
     7141 gcgaggatat tcccattaca gcacggcgcg tggtgacctt cagacccggg cagaagttaa
     7201 aaagccgggt cgaaaacgct tcgcccaaag acgagtaatc tgatctaact aaaaaggccg
     7261 ctatgcggcc ttttttcttt tcactgtcga agagtcaccg taaaatcaac gccatgacac
     7321 ttcagcagaa cggataccat gctgacactt gcccgccaac aacagcgaca aaatattcgc
     7381 tggttattat gcctgtcagt tttgatgctg ctggcgcttc tcttaagcct ttgcgccggt
     7441 gaacaatgga tttcgccagg tgactggttt actcctcgtg gcgaactgtt cgtctggcaa
     7501 attcgcctgc cacgtacgct ggctgtattg ctggttggtg cggcgctggc tatatccggc
     7561 gctgtaatgc aggcgttgtt tgaaaatcct ctggcagaac ctggactact tggcgtctct
     7621 aacggcgcag gcgtggggct tatcgccgcg gtattgcttg ggcaagggca actccccaac
     7681 tgggcgctag ggctgtgtgc gattgctggc gcgcttatca tcactttaat actcttacgt
     7741 ttcgcccgtc gtcatctttc gaccagtcgg ttattgctgg ctggcgttgc attagggatt
     7801 atctgcagcg cactaatgac gtgggctatc tacttttcca cctctgttga tttacgtcag
     7861 ctgatgtact ggatgatggg cggttttggc ggcgtagact ggcggcaaag ctggctgatg
     7921 ctggcattga tccccgtgtt gttgtggatc tgttgtcagt ccaggccgat gaatatgtta
     7981 gcgcttggcg agatctcggc gcggcaactg ggtttaccca tgtggttctg gcgcaatgtg
     8041 ctggtggcag cgaccggctg gatggttggc gtcagtgtgg cgctggcggg tgctatcggc
     8101 tttattggtc tggtgatccc acatattctt cggttgtgtg gtttaaccga tcatcgcgta
     8161 ttacttcccg gctgcgcgct ggcaggggcg agcgcattgc tgctggccga tattgtagcg
     8221 cgcctggcat tagctgccgc agagctgcct attggcgtgg tcaccgcaac gttgggtgcg
     8281 ccggtgttta tctggttatt gttaaaagca ggacgttagc cgcaaaaaga cggtctatga
     8341 ttaaaagcta agatttttac tgaccacacc taggagaaac gatgcaagat tccattctga
     8401 cgaccgtagt gaaagatatc gacggtgaag tgaccacgct ggagaagtac gccggtaatg
     8461 tgctgttaat tgtcaatgtc gcctcaaagt gtggcttaac gccgcaatat gagcagttgg
     8521 agaatattca aaaagcctgg gccgatcgag gttttgtggt gctgggattc ccgtgcaacc
     8581 agtttctgga acaagaaccg ggcagcgatg aagagattaa aacttactgt accaccacat
     8641 ggggggtgac gttcccgatg ttcagtaaga ttgaagttaa tggcgaagga cgccatccgc
     8701 tgtatcaaaa attgattgcc gcagcgccga ccacagtcgc gccggaagag agcggattct
     8761 atgcccgtat ggtcagcaaa ggccgtgcac cgctgtaccc ggatgatatt ttatggaatt
     8821 ttgaaaaatt cctggttggc agggacggaa aagtcatcca gcgtttttcc ccggatatga
     8881 cgccggaaga tcccattgtg atggaaagca ttaaactggc gttggcaaaa taatgtctat
     8941 tgtgatgcag ttacaggatg ttgcggaatc tacccgcctg gggccgcttt caggcgaggt
     9001 tcgggctggg gaaatcctgc acctggtggg gccgaatggc gcgggtaaga gtaccttact
     9061 ggcgcgaatg gccggaatga ccagcggtaa gggaagcatt cagttcgcgg ggcaaccact
     9121 ggaagcatgg tccgcaacaa aactcgcgct gcatcgcgcc tatctttcac aacagcagac
     9181 gccgccgttt gcaatgccgg tctggcacta cctgacactg catcagcacg ataaaacgcg
     9241 taccgaacta ctgaatgatg tcgcaggggc gctggctctt gatgacaaac tcggtcgtag
     9301 caccaatcaa ctttccggcg gtgaatggca acgcgtacgt cttgctgcgg tggtgttgca
     9361 aatcacacca caagccaatc ccgcaggcca attgctgctt cttgatgagc cgatgaacag
     9421 tcttgatgtt gcgcaacaaa gtgcgttaga caaaattctg agcgcgctgt gtcagcaagg
     9481 actggcgatt gtgatgagca gtcacgatct caaccacacg ttgcgtcatg cgcatcgggc
     9541 gtggttgcta aaaggtggaa aaatgctggc cagtggacgc agagaagagg tgctcacgcc
     9601 gccaaatctg gcgcaggcct atgggatgaa ttttcgccgt ctggatatcg aaggtcacag
     9661 aatgctgatt tcgaccatct gatttcatta gtatcccgaa aatcgggtaa ttacgacgct
     9721 aaattaatgc aagaataaaa aacagaggaa ccgtacggaa tgcgtttctg ccttattttg
     9781 atcacagcac tgtttctggc cgggtgtagc caccataaag caccgccgcc aaatgccaga
     9841 ctttctgatt cgattaccgt tattgccggt ttgaacgacc agctacaaag ctggcatggc
     9901 acgccgtatc gttatggtgg catgacgcgg cgcggtgtgg actgttcggg atttgtggtt
     9961 gtgacgatgc gcgatcgttt cgatttgcat ctgccccgtg aaaccaaaca acaagcctct
    10021 attggcacgc aaattgataa agacgagttg ctgcctggtg acctggtctt tttcaaaacg
    10081 ggttccggac aaaatggttt gcatgtaggt atttacgata ccaacaacca atttatccac
    10141 gcctctacca gcaagggagt gatgcgttcc tcacttgata atgtctattg gcagaaaaat
    10201 ttctggcagg cgagacgaat ctagtatgtc gaaaaattga cggcgatgat acatcgccgt
    10261 caatttactc ctgaatacag tagtattttg cagacaaaaa ttccgtaaat ggattattcg
    10321 tttataatca ggataatcta aaaaaaggcc ccctgaaaaa gtggttaaca tggaacaaat
    10381 gaaagttact ccttctgtaa ttgttatgtc ttatggcctc atccctttta gccggatact
    10441 gaaaaacatc cttcgagagg gacggttacc atgaagattt ttttggagaa tctttatcat
    10501 tctgattgct atttcctgcc gataagagat aaccagcaac tccttgtcgg cgttgagtta
    10561 attacccatt tctccagtga agatggcacg gttcgtatcc cgacgagtcg ggttatagcg
    10621 caacttaccg aggagcaaca ttggcaatta ttttcagagc agttggaatt actaaaatca
    10681 tgccaacact tttttattca acataaactc tttgcctggc ttaatttaac gccacaagtg
    10741 gctacattat tattagacag ggataattac gcaggcgaac tattaaaata tccgtttatt
    10801 gagctgttga ttaatgagaa ttacccgcat ctcaatgaag gaaaagataa tcgcgatctt
    10861 ttgtccttat cgcaaatgta tccccttgtg ttaggcaatt tgggagcagg taatagcaca
    10921 atgaaagcgg tttttgatgg tcttttcacc cgcgtaatgc tggagaaagg ttttattcag
    10981 caacaaatca cgcatcgctc cttcgaaccg tttattcgtg cgatccaggc gcaaatttcc
    11041 ccttgttgca attgcatcat cgcggggggc attgatacgc cagaaatatt ggcacagatt
    11101 acccccttcg atttccatgc tctccaggga tgcctgtggc ctgccgttcc tataaatcag
    11161 ataacgacgt tggttcagcg ataaccctcc tgtttgctgg tgtttaagac gagagtaacc
    11221 gtctacacta tcaaacagat aggatctatg accctgtctt ttattacccg ctggcgcgat
    11281 gaattgccag aaacctatac agcactttcc cctacgcctt taaataatgc ccggctgatt
    11341 tggcataata ccgaactcgc taacacgctg agtattccat cgtcgctgtt taaaaatgcc
    11401 gcaggtgtct ggggcggcga aaccctactg cctggtatgt caccactggc acaggtttac
    11461 agtggtcatc agttcgtcgt ctgggcgggc caactgggtg atgggcgcgg cattttactt
    11521 ggcgaacaac tgcttgctga tggcactaca atggactggc atctgaaagg tgctggcctg
    11581 acgccttatt cgcgaatggg tgatggacgg gcggttttac gttcgacgat acgagaaagt
    11641 ctcgccagtg aggcgatgca ttatctgggc attccgacga cccgcgcgtt aagtatcgtc
    11701 accagcgatt cgccagtgta tcgggaaacg gtggagccag gcgcgatgct gatgcgtgtg
    11761 gcaccaagtc atctgcgctt tggtcatttc gaacattttt actatcgccg cgagccggaa
    11821 aaggttcgtc agttggctga ctttgccatt cgtcattact ggtcacatct tgaagacgat
    11881 gaggacaaat accgtctctg gtttaacgat gttgtcgcac gtaccgcgtc gttaattgcc
    11941 caatggcaga cggtcggctt tgcacatggg gtgatgaata ccgacaacat gtcgctgctg
    12001 ggactgacgc ttgattacgg gccgtttggt tttcttgatg attacgaacc cggttttatt
    12061 tgtaatcact cggatcatca agggcgttat agctttgata atcaacctgc tgtcgcgttg
    12121 tggaatttac agcgtctggc gcagacattg tcaccatttg ttgccgtaga tgccctgaat
    12181 gaggccctgg acagctatca gcaggttttg ttgacgcatt atggacaacg gatgcggcag
    12241 aaactgggct tcatgacgga gcaaaaagag gataacgcgc tactgaatga attattcagt
    12301 ctgatggcgc gagagcgcag cgattatacc cgcacattcc gcatgctgag tctgaccgag
    12361 cagcacagcg cggcgtcacc gctacgtgag gagtttattg atcgtgcggc atttgatgac
    12421 tggtttgccc gttatcgggg gcgtttgcaa caagacgagg ttagcgatag tgagcgtcag
    12481 caactgatgc aaagcgttaa cccagctctg gtattgcgca actggttggc gcaacgggcg
    12541 attgaagcgg cagaaaaggg tgatatgatg gaattgcacc gcctgcatga ggcgttgcga
    12601 aatcctttca gcgacagaga tgatgactat gtcagtcgtc cacctgactg gggtaaacgg
    12661 ctggaagtca gctgctcgag ttaaaggcta cttagttaac agcagcttgc cagcctgagt
    12721 tttacggagc agatattctt gcccgtcatg atcgataatc agtttgccat ccggacctaa
    12781 cagtgtctgg ctggtaattc gccggatggg ctgcggggaa gcggtcttgt gattggcatc
    12841 cgtttcaggc gtgagttttc tgctatccgt ataacgcata aaaattaata ataattgata
    12901 taacaatgat tcctattatc aatattatgg cagaccagag caagcagtaa tgtgttgaga
    12961 agacaacaag ttgcgcagaa atgcccatcc catcaggaat gggcacgcat tcagaagcgg
    13021 gtatctaccg caaaggcgag tttttcgacc aggcgttcgg tatcttccca gcccagacac
    13081 gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc cgacgatttt ttgcgttcct
    13141 tcgcgcagga aactttccgc cataattcca gcaatctccg tagagccatt gcggatttgc
    13201 tgacaaatat cctcacaaac ttctaactgg cgacggtgct gcttctggca gttaccgtgg
    13261 ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc gcaggctgcg
    13321 gcgatatcat cggcatgata attcggtttt ttgccgccac gcataataat gtggccatac
    13381 gggttgccgc tggtctgata gatggtcatc tgaccatttt tgtctggcga gaggaacata
    13441 tggctggcgc gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc atcggtacca
    13501 tttttaaaac ctaccggaca ggagagtgct gaagccattt cgcggtggat ctgactttcg
    13561 gtagtacgag cgccaatcgc gccccaactg attaaatcag caataaactg accggttacc
    13621 atatcgagga actcagtcgc ggtagggaca cccagctcat ttacctgtaa aagtaatttg
    13681 cgcgccagct ccagaccgtg atttacccga tagctgccgt ttaaatccgg atcggagatt
    13741 agccctttcc agccgacaac agttcgtggt ttttcaaaat aggtgcgcat tacgatttcc
    13801 agccgtgact ggtactggtt gcgcagcgac tgcagacggg tggcgtactc cattgcagcg
    13861 gtgagatcgt ggatcgagca ggggccaata atgaccaaca gtcgcttatc ttcaccattc
    13921 agtatttttt caattctgcg gcgggagtcg gtgacatggg tggcgacgcc aggcgttacg
    13981 ggataccgta gcgcgagttc ggcgggcgtt accaggctct caatacgcgc agtacggagt
    14041 tcgtcagttc tgttcattac aagtctcaga aatttgttgc ttctacggca ggactaccgg
    14101 aaagtgatgc gcatcacgat aaaccaatcg cagctgattt caagtgcgag gtgtgtcaca
    14161 cctcgccggg tggttagcat gataacaaaa aaacaaacca atgcactagt tctctagtac
    14221 attcggcgac taaggcccat gatatcgagg atcttggtgg caatctcttc taccgaataa
    14281 ttggtactgt taatccacgg gatctgattt ttacggtaca aggcttccac ttccgcgact
    14341 tccatcctgc actgacgaag cgaggcatag cgactgttct cccgacgttc ctcgcgaatc
    14401 gccgccagac gttccgggtc gatagtcagg ccgaacaatt tatgctgaag cggtttgagc
    14461 gacgcgggta gcaccagatt atccatatcg tcggcaataa aggggtagtt tgccgcgcgg
    14521 ataccaaatt gcattgccag atacagactg gtgggggttt taccacagcg agaaacaccg
    14581 agcaggatca cctgagcctg atccagattg cgcaacgaaa tgccgtcatc gtgggcgagg
    14641 gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga gattattagg gttaaggcca
    14701 tgggtacgat gagcaatcgg cgttggatcc agtttcatct cttgttgtag cggggcaacc
    14761 agcgcctgaa cgatatcctg gcaaaagcct tcactttgca agatgatggc gcgaatctcc
    14821 ggcaacacga tggagtagaa gaccagcggg cgcacgcctg tctggtgata aattgcgtca
    14881 atctgatcct tcactgcccg tgcacggctc tcattttcga caaacggcag cgtgatgctg
    14941 ctgatagtga cgggaaattg tgacattact gcgtgtccta atacctccgc agttattgcc
    15001 gtaccatcag aaatataaaa aacgtggcga tcaacagcat tatccatttt gttcttcccg
    15061 tgaagcagac ataattctat gaaagcataa attaaaagcg cacagaagcg taggacgtaa
    15121 tgtctggttt ataaaatgaa ccttcatttt attttttata aaaacagcat ttcattttta
    15181 tggtttcgtt tataccgatg gtttatgtgg aaattgtcga atagagcagg tttgcgcaac
    15241 gctgggatca gtcttaaaaa gtaaaaaaat atatttgctt gaacgattca ccgttttttt
    15301 catccggtta aatatgcaaa gataaatgcg cagaaatgtg tttctcaaac cgttcattta
    15361 tcacaaaagg attgttcgat gtccaacaat ggctcgtcac cgctggtgct ttggtataac
    15421 caactcggca tgaatgatgt agacagggtt gggggcaaaa atgcctccct gggtgaaatg
    15481 attactaatc tttccggaat gggtgtttcc gttccgaatg gtttcgccac aaccgccgac
    15541 gcgtttaacc agtttctgga ccaaagcggc gtaaaccagc gcatttatga actgctggat
    15601 aaaacggata ttgacgatgt tactcagctt gcgaaagcgg gcgcgcaaat ccgccagtgg
    15661 attatcgaca ctcccttcca gcctgagctg gaaaacgcca tacgcgaagc ctatgcacag
    15721 ctttccgccg atgacgaaaa cgcctctttt gcggtacgct cctccgccac cgcagaagac
    15781 atgccggacg cttcctttgc cggtcagcag gaaaccttcc tcaacgttca gggttttgac
    15841 gccgttctcg tggcagtgaa acatgtattt gcttctctgt ttaacgatcg cgccatctct
    15901 tatcgtgtgc accagggtta cgatcaccgt ggcgtggcgc tctccgccgg tgttcagcgg
    15961 atggtgcgct ccgacctcgc atcttctggc gtgatgttct ccattgatac cgaatccggc
    16021 tttgaccagg tggtgtttat cacttccgca tggggccttg gggaaatggt cgtgcagggt
    16081 gcggttaacc cggatgagtt ttacgtgcat aaaccgacac tggcggcgaa tcgcccggct
    16141 atcgtgcgcc gcaccatggg gtcgaaaaaa atccgcatgg tttacgcgcc gacccaggag
    16201 cacggcaagc aggttaaaat cgaagacgta ccgcaggaac agcgtgacat cttctcgctg
    16261 accaacgaag aagtgcagga actggcaaaa caggccgtac aaattgagaa acactacggt
    16321 cgcccaatgg atattgagtg ggcgaaagat ggccacaccg gcaaactgtt cattgtgcag
    16381 gcgcgtccgg aaaccgtgcg ctcacgcggt caggtcatgg agcgttatac gctgcattca
    16441 cagggtaaga ttatcgccga aggccgtgct atcggtcatc gcatcggcgc gggtccggtg
    16501 aaagtcatcc atgacatcag cgaaatgaac cgcatcgaac ctggcgacgt gctggttact
    16561 gacatgaccg acccggactg ggaaccgatc atgaagaaag catctgccat cgtcaccaac
    16621 cgtggcggtc gtacctgtca cgcggcgatc atcgctcgtg aactgggcat tccggcggta
    16681 gtgggctgtg gagatgcaac agaacggatg aaagacggtg agaacgtcac tgtttcttgt
    16741 gccgaaggtg ataccggtta cgtctatgcg gagttgctgg aatttagcgt gaaaagctcc
    16801 agcgtagaaa cgatgccgga tctgccgttg aaagtgatga tgaacgtcgg taacccggac
    16861 cgtgctttcg acttcgcctg cctgccgagc gaaggcgtgg gccttgcgcg tctggaattt
    16921 atcatcaacc gtatgattgg cgtccaccca cgcgcactgc ttgagtttga cgatcaggaa
    16981 ccgcagttgc aaaacgaaat ccgcgagatg atgaaaggtt ttgattctcc gcgtgaattt
    17041 tacgttggtc gtctgactga agggatcgcg acgctgggtg ccgcgtttta tccgaagcgc
    17101 gtcattgtcc gtctctctga ttttaaatcg aacgaatatg ccaacctggt cggtggtgag
    17161 cgttacgagc cagatgaaga gaacccgatg ctcggcttcc gtggtgcggg ccgctatgtt
    17221 tccgacagct tccgcgactg cttcgcactg gagtgtgaag cagtgaaacg tgtgcgcaac
    17281 gacatggggc tgaccaacgt tgagatcatg atcccgttcg tgcgtaccgt agatcaggcg
    17341 aaagcggtgg ttgaagaact ggcgcgtcag gggctgaaac gtggcgagaa cgggctgaaa
    17401 atcatcatga tgtgtgaaat cccgtccaac gccttgctgg ccgagcagtt cctcgaatat
    17461 ttcgacggct tctcaattgg ctcaaacgat atgacgcagc tggcgctcgg tctggaccgt
    17521 gactccggcg tggtgtctga attgttcgat gagcgcaacg atgcggtgaa agcactgctg
    17581 tcgatggcta tccgtgccgc gaagaaacag ggcaaatatg tcgggatttg cggtcagggt
    17641 ccgtccgacc acgaagactt tgccgcatgg ttgatggaag aggggatcga tagcctgtct
    17701 ctgaacccgg acaccgtggt gcaaacctgg ttaagcctgg ctgaactgaa gaaataaaat
    17761 aaatccccgg cggcgttcag tcgccggggt tatgtgatcc ccgaagatga aacttattca
    17821 atctcttcac agacatcctg cgttaatcgc cgtataatat cttttcttaa caaaaacttt
    17881 tgtattttat ctgaggcagt gcgcggtagt ttttcgatta ccacgatatg ttcaggatat
    17941 ttatattttg cgacccgttt acggctaaaa aaagccgcca tctcttccag cgataatgaa
    18001 tgatgcggcg ctttcagcac gacataagcg catgatcgtt cacctaaacg ttcatcgggc
    18061 attgcaacca cacaggcatc gtgaatttta ggatgctgca ataaaatatc ttccacttca
    18121 cggctgctaa tattttcgcc gccgcggaca ataatatctt ttttgcgtcc ggtaattttt
    18181 atatagccag cctcatccat acggcagaga tcgccgctgt aataccagcc ttcttcatcc
    18241 agcgcacggg cggttaattc aggttcatca aaatacccca taaacacatt ggggccacgc
    18301 gatgcttctt caccttcgca acctggcggt aaggtcttgc gtgcgtcatc gaccacttta
    18361 atctctacac ctgcggcagc gtaaccatcg gtatgcataa agcgcgacaa agaattatcg
    18421 agattcacca ccgcatgcgg cgaactttct gtggaaccat aaacacttaa taatttaatg
    18481 ctgcgctgct ggcattcgcg cgccactttt ttggggattg tggtaccgcc gcaaagaaag
    18541 aagcgcagcg ctgaaaggtc tgcgggttgt ttctctagta aattcaaaag atcatagaca
    18601 aacggcgttg cgcctagcat acaggtgcaa cgctgctgct caagcagcgc gagacacgca
    18661 tcaggagtga aaatatctaa caacacgctg cgagcgccaa ttaagaatgg tgccgttacg
    18721 ccatgcagaa agcccgttgc gtgaccaagt ggcgcaggca tcataaagac atccagccag
    18781 gtcagattca gccgcgcgca ataagcccgc tcactggcga gaatattgtt atgcgttagc
    18841 atcacgccct ttggcagacc ctcggttccg gaggtaaaca gcaccgcagc taattcatcg
    18901 ccgtgggtcg ttatcgccgt ggtcagtggg atattgtcgg cgataatctg acttaatgag
    18961 agggaagagg tggcgggagc cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc
    19021 tgattttgca gcggcaggat taaatctacc ggacgcgttt gtttaaacaa cgtcggtgca
    19081 aagaacattt ttgcctgaca cttattgagc acccacacca gttctgcttc ccgccaggaa
    19141 ggcaacagcg gcacggaaac cgcaccgatt ttcaggcagg caagatagat aacggtaaat
    19201 tcacaccagc caggcagttg aaatgcgatg cgatcgcctg attcaatacc cttcgctaac
    19261 atccagtttg ccagacagct cgcggcgtga tcgagcgcgc tataggtgta cgatgcacca
    19321 tgattatcga ccacggcaat tttgtctggc atcgcacgag cggtctgctg ccagtaatcg
    19381 gccagtgaag catcgcccca tagcccttgc tgacgatacg ccgcacgacg ttgttcgtta
    19441 aacgttaatg tcactttcat tttggactct cgaaacagaa catcaggccc gagttgcggg
    19501 cctgtgggat gcatgattag ccgtagcgaa aatcaatacc gaaggtgcct gcgggatatt
    19561 gccactgttc gagaatagtg ttaccgcaca gcacccgaca ggtgccgcac tccagacaac
    19621 cggcgggatc aaaatgaatg tttcctacgt catcctgctt ataaagtccg gcagggcagg
    19681 ctttcattaa tttacggaat tcattgatat cgggattttc cgtcagaatg atatgcgggt
    19741 ggccctcatc aacatggaat ttattgacgc ctaatttgat gtcgacgtta accgtagcgt
    19801 tctggctcat agcgcggttg ctcccttaat gccatctttc agcaagttga tcagcccaat
    19861 tttcttcgcg tgtcccatga tcattttgcg taccggctgg tttggtttgc cgtcaatggt
    19921 gaacatctcg ttcatgatgt cggctaccat tcgtgggtat tggctaaaca ggcgcgggtt
    19981 ttccatcagc gccgggatct tgcgaaaatg ctgcatatca cgcatgacgc agctttgttc
    20041 cagctcacgt ttgtattgcg ccagactgtt ggcggagaaa tccgcgcgtt ctttggcggc
    20101 gatcaccgtt gtggcggcag cctgagccga tgcaatggct aaatccatgc cgcggaccgt
    20161 aaaacccaaa ttcaggcaga agcctgcggc gtcaccaacg atcatcacgc cctcgttaac
    20221 cagttgcggc accattgcca gaccgccttc cggcaccata tgcgcggaat attcaagcag
    20281 tttgccgccg ctaatcagcg ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg
    20341 cggcacgctt ttttgcgcat gggcgatatc acccaatcca caaaccagcc ccaaggatat
    20401 ggaatccttg ttggtataga gaaatccccc gcccatcagg ccgtcagaag gggaaccggc
    20461 aaacagccag gcggcacctt cctcgcccgt aatattaaag cgatcgttga tctgttctgg
    20521 tgtgaggcca ataacctctt taacaccaac agcgtaatga tgcggatcgg aagcgggaac
    20581 cattcccagc gagcggccaa gcatcgagtt aacgccatca gccagaatca ccacattcgc
    20641 ttcgagaata tcatccccag cctgcacgcc agtgaccttg tttccttcac gaaccagcgc
    20701 gtcgacgcga acgcctggga taaactgcgc gcccgcctgc tcggcttgtt ccatcaacca
    20761 cgggtccaga cgattacgca atacggtata agatgcgtgt tgaggaacat ctggttgctc
    20821 gcggtgaaaa tcgagggtaa cggcgctttc ttcggttaag aaggagattt tctcgcgtgt
    20881 gaccttgcgt tctaccggcg ctgatactgc aaagcctgga atgattgctt caagtgtgtg
    20941 ggcataaaga cgcccgccgg tcatgttttt acatccggca ctgtcgccgc gttctatcac
    21001 caggacatcc agcccggctc gtgccatgac cagtgcggca acgctaccag caacgcccgc
    21061 accgaccaca atggcatcaa atttgtcatc cgacatgcgt tatgctccct tcctgctgcg
    21121 tttacagcgg gagatgtggt tgaaagggga attagcggct caactggcta atcagggcag
    21181 ggacgacttt gtagatatcg cccaccagac cgtagtcggc atagttgaag attggcgcat
    21241 ttttatcttt attgatggcg acaatcactt ttacgccgtt gccgccaacc atatgttgga
    21301 tctgcccgga gatccccagc gtcaggtaga gatcggattt cagcaacacg ccggagacac
    21361 cgatataacg ttcacgctcc atccagttct cgccttcggc aattggacgt gaacagccga
    21421 cttcagcatt cagcaccgcc gccagttcgt ggaccatttt caggtcatcc tgcgccgcca
    21481 gaccacgacc gacgccaacc acacgttttg ctttgctcag gtccacgctg cttgcggctt
    21541 tcgcacggcg ttcgcgacag agaatttcat gacgcggagc aacataaggt accgtttctg
    21601 tcgggcactg atgagaggta tcactggtgc acggttcctg aacaccgggt gcaagggtaa
    21661 tgatcgccag cgggctgttg atcttttcct gagcgaacgc taacccgcca tacatccggt
    21721 gttcggcgca aatgtgacca tcgacaatat ccaccgccgt ggcatcgttc accagcgccg
    21781 cattcagttg cacacttaac cgtgctgcca gagctttacc acgtttcgtc gcggccaaca
    21841 gcaacatagc ggggtgttta tctttcagca gggcagcaat gctttcggcg taattttcag
    21901 tgcgttgcag cgcgtcgttt tgctcaagaa cataaataca ttttggacca taaggcataa
    21961 ctgcctgcgc ctggtcggta ttttgtacaa tggcatacac ctgttggccc cattgctgag
    22021 cgccgccaaa cagttcagca taacgttcag gattatcgct aaagacccag acgctgttta
    22081 attgactcat cgtttcttct ccgtaggctt cagttcaggg ctttcttcag atgttccgcc
    22141 agctcggcaa tggcctccgg cgaatcgtta tcgagaatga tgtgcttacg ttctgtttgc
    22201 ggcggtacgc gaatgccgac cagttcagca agtggcgcgc tctggctcca gtcaatatca
    22261 cttgcctgcc actgatttac cggtttttta cccgcgccga gaatggcttt catcgaagga
    22321 atgcgtggca cgttaatatc ggaggtgacg cagagcacgg ctggaacaga gagttcaata
    22381 acttcaacat catcttcaag cgtgcgttca atcaccagtg tattgccctg acgctgaata
    22441 gcactcactg cattaatcac cggaagttgc agaatttctc cgaccagcaa gccaacctgc
    22501 tgggcataaa ggtcgccgga accttcacca aagatcagta aatcgaagcc gatcttttca
    22561 attgctgccg ccagcgcttt tgcggtatcg agaggcagtg catgttcaag ttgcgcatcc
    22621 tgcaccaaat acaggctgtg cggcccgcgg gatagcacgt ctttgcgcac tttcgagttc
    22681 tgcaacaatg agccgccaac ggtcagcgcg gctatctcat cgtcatctgt tgcgagctgg
    22741 cttgcagctt caatggcatt gagatcgaac tggctgattt tggcgtcggc attgtcgaaa
    22801 ttcagggtgt attctggagt gacaacaatg tcctgttctt caggcaccag cttaaagcag
    22861 gttattattt tcatgattt
//
LOCUS       DAJTUP010000061        22233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTUP010000061 DAJTUP010000000
VERSION     DAJTUP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.061"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..916)
                     /locus_tag="OO468_002457"
     CDS             complement(<1..916)
                     /locus_tag="OO468_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259537.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLI"
     gene            complement(1018..1998)
                     /locus_tag="OO468_002458"
     CDS             complement(1018..1998)
                     /locus_tag="OO468_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR6259538.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2006..2716)
                     /locus_tag="OO468_002459"
     CDS             complement(2006..2716)
                     /locus_tag="OO468_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR6259539.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(2933..4756)
                     /gene="typA"
                     /locus_tag="OO468_002460"
     CDS             complement(2933..4756)
                     /gene="typA"
                     /locus_tag="OO468_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR6259540.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5129..6538
                     /gene="glnA"
                     /locus_tag="OO468_002461"
     CDS             5129..6538
                     /gene="glnA"
                     /locus_tag="OO468_002461"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR6259541.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6711..7760
                     /gene="glnL"
                     /locus_tag="OO468_002462"
     CDS             6711..7760
                     /gene="glnL"
                     /locus_tag="OO468_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR6259542.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7772..9181
                     /gene="glnG"
                     /locus_tag="OO468_002463"
     CDS             7772..9181
                     /gene="glnG"
                     /locus_tag="OO468_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR6259543.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9293..9403
                     /locus_tag="OO468_002464"
     CDS             9293..9403
                     /locus_tag="OO468_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR6259544.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9632..11005)
                     /gene="hemN"
                     /locus_tag="OO468_002465"
     CDS             complement(9632..11005)
                     /gene="hemN"
                     /locus_tag="OO468_002465"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR6259545.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11194..11703)
                     /gene="yihI"
                     /locus_tag="OO468_002466"
     CDS             complement(11194..11703)
                     /gene="yihI"
                     /locus_tag="OO468_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR6259546.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12285..12917
                     /locus_tag="OO468_002467"
     CDS             12285..12917
                     /locus_tag="OO468_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR6259547.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13299..16085)
                     /gene="polA"
                     /locus_tag="OO468_002468"
     CDS             complement(13299..16085)
                     /gene="polA"
                     /locus_tag="OO468_002468"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR6259548.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16449..17381
                     /locus_tag="OO468_002469"
     CDS             16449..17381
                     /locus_tag="OO468_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR6259549.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17422..18852)
                     /locus_tag="OO468_002470"
     CDS             complement(17422..18852)
                     /locus_tag="OO468_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR6259550.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYSPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19007..19633)
                     /gene="dsbA"
                     /locus_tag="OO468_002471"
     CDS             complement(19007..19633)
                     /gene="dsbA"
                     /locus_tag="OO468_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR6259551.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19650..20636)
                     /gene="srkA"
                     /locus_tag="OO468_002472"
     CDS             complement(19650..20636)
                     /gene="srkA"
                     /locus_tag="OO468_002472"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR6259552.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20713..20982)
                     /locus_tag="OO468_002473"
     CDS             complement(20713..20982)
                     /locus_tag="OO468_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR6259553.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21052..21636
                     /gene="mobA"
                     /locus_tag="OO468_002474"
     CDS             21052..21636
                     /gene="mobA"
                     /locus_tag="OO468_002474"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR6259554.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21618..22145
                     /gene="mobB"
                     /locus_tag="OO468_002475"
     CDS             21618..22145
                     /gene="mobB"
                     /locus_tag="OO468_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR6259555.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5500 a         5572 c         5320 g         5841 t
ORIGIN      
        1 taatcaacag tacgagcgca gcggtactga tgataaaggt gtagcataga tatttactcg
       61 gcgatttcag gatcttatct gaaatcatgc caccaatcgg tccaccaatc attttcagac
      121 agtattggtt aatgatgccg taagccccca ccagcgcaac gggcaataga tagatgtttt
      181 tcaggaatgg aatgaagaat gtcaggccac agtaaaccgc gtaaacgaag aagacgttga
      241 aagcgataag ccagatggtt ttgtctctca gcactgaggt catactggtg ttttgcgatg
      301 ctccgtcttc ttttttcacc tcaacggacg gtgcctcttc tttgtcattc aggacaaaga
      361 aaataataat gcctacggca atcacaataa gggaatagaa ccagatgcct gctttaaaac
      421 ctaataagcc actgccaaac caggtaaata ctgccaacgc agaaaatgcc accacggtat
      481 cgacaatgcc acgccctgtt tcgaagaagc caaacaaccg accttgttgt tcgctattgc
      541 ccaatcgact taccgacttg agcaagaccg gccagttcat catgtcgcaa gtaacgccaa
      601 atagcgccca gacaaagagg atgccccaat accccggcat tgtcgtcaaa taaacaccca
      661 gtaatcctgt cgcaatgagt gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa
      721 aataaataga cagaaaaaag cccactgtgg tgacaaatga gtttaccgac atagcattac
      781 caatttgacc attggtcaaa tggaaatatt cctgcatcgg gatataaaac gcatctttca
      841 gcgacggtaa tttataaatt gtaccgccac acagtgttaa tagactaaat aacgcccatt
      901 tctttttcgt gagcataaca tcccctaagc gatttttaaa atcactgttt taaaggcaat
      961 aagataccga ccattcatat ggaatggttt tattaactgc gtatgagtta aaacttatta
     1021 gtggttcaga agatggtttg cctcttgttt gatttgttgt agtacgttac gcacatgatt
     1081 tatctgatta attgcatctt ctttttcttt acgtaaccgc gcatccagta aatgattttc
     1141 tggtaggggt gtttcactca tctggcgata agggatccac ggattatcat cttcgtcttc
     1201 aaagcggaac gccgacgcat agtaatcaac ctcttcttcc aggccatatg ccgtcacctg
     1261 cggctcatca tcgccaaggc agataaggtg cgttaataac gctttgaatg gcatatcacc
     1321 ctgacctgaa atacaggctt tatgacccag gccaccctgt tctttaacga tcaaggcatc
     1381 tttaatatgg acctgggtaa tatgcggtgc catcgttttt aaggcgtcga tgggatgctc
     1441 atttgcattg atcatgttag caaaatcaaa cagtaaggaa agagactcca tctcactttc
     1501 tttgaccagc gacaccagct catgactttt taaatcttca tgctgctcaa tcgtaaaagt
     1561 caggccgctg tcctgatacg tttcccgtac ataggcaatg tcgttagcga taatcgataa
     1621 tacgtcgcgc aaattacctt cataacatgg ataaaaacgt acggacgttg ccccagtttt
     1681 caacgcgatg gcgacggctt cgtcaataga tgccttatct gaagcgctgg tttcaatatg
     1741 aatatcaagg ttcagacggc gggctttatc cccaaaggca gagagttctt tatcgtccat
     1801 attaccaaga gaaaaacgct cgccatccag aacatggatt ttgacgccac gtagattatt
     1861 ttcactggcg atatcaagta aatcagcagg caacactctt tccagccgca tatttaaatg
     1921 aaaggcataa gcatgtaaat aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc
     1981 gttatttatt gtcaccataa aacacctaca gattaatgat gaggtctacg gcctcaaatc
     2041 gccaatattc aatatctatc tggacaatat tgccttgctg gtcggcgcga tgcttttcaa
     2101 ttaagatcgc tggcataccc gcagaaccgc caagatactt gctgatatcg cccggcattc
     2161 tgacgggttt gaaaatcaat ttttttacta ccgtctcttt ttggtagcac ttttcccaga
     2221 ctgcagaaaa tgagtggttt tcaagttgtt caataaaacc cggagcaact tcaggattaa
     2281 tatatgtttc gtgatagaaa actttatgtc cttccagcgc cccccagccg gtgatgcgat
     2341 agagttcagt tgatggcgtc actgcaatca gtggcgcgag cgtctcggga atatcgctgg
     2401 tacggttttt ctcggtaaac ccccaagaag gctctcgccc ttgctcaatt gcggcccgct
     2461 gaaagctcgc cgacagctcc ggactgtaat taaatcgcgg ctgggtcacg aaccacccct
     2521 tacgatcctt acggaagatt ttggattctg cctcgaggtt caacaacgcc tggcgcagcg
     2581 tcatacgttt aatgcccagc agttctccca gctctctttc tgaagggagt tttccacccg
     2641 gcgtggtgat gccatctttc aaccaccgat ccagtttctc ttttgcattt tctacggtgg
     2701 attgattctc cgccattgtt taatcctttt ggtttaaacc agatgaagca tcttacgctt
     2761 acgccaaaaa agaaagcaat taacggtgta tgactttgca gataattgtg acaaagctct
     2821 ctattgacgt aatcacagac ttataaggga gatactcgca ttggtgtgcg ctcacgacag
     2881 gtaaaaaaaa acctgccatc gctggcaggt tttttatgac taaaaaacga aattaatcgt
     2941 ctttcggtgc gcggttggcg cggcgacgat cgttttccgt caggtgacgt ttacgaatac
     3001 ggatagaggt cggagtcact tctaccagtt cgtcatcatc gatgaactcc agagcttgtt
     3061 ccagagtcat gcggataggc ggaaccagaa caacagcttc gtcagtaccg gaagcacgca
     3121 tgttggtcag tttcttaccg gtcaggcagt ttacagtcag gtcgttagag cggctatgaa
     3181 taccgataat ctgaccttcg taaacttctg caccgtgacc gaggaacagc ttaccgcgat
     3241 cctgcagacc gaacagcgcg aacgcgacag ctttaccctg accgttagag atcagtacac
     3301 cgttctgacg ctgacccact tcgcccggac gtacgtcgtc gtagtggctg aaggtggagt
     3361 acagcagacc agtaccggaa gtcatggtca tgaactcaga acggaagcca atcagaccac
     3421 ggcttgggat cacgtagtcg agacgtacgc ggcctttacc gtctggattc atgtttttca
     3481 ggtcgccttt acgttcgccc agcgcctgca tcacagaacc ctgatgctgt tcttcaacgt
     3541 ccagcgtcac gttttcatac ggctcttgtt tacgaccgtc tatttcacgg aagattactt
     3601 tcggacggga taccgccagt tcgaaacctt cacgacgcat gttttcgata agaacagaga
     3661 ggtgcagttc accacgacca gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc
     3721 gcagcgcaac gttgtgtacc agttctttgt tcagacgatc gaggatctga cgagacgtta
     3781 cgaacttacc ttctttaccg cagaacggcg aggtgttaac gcagaagaac atagaaacgg
     3841 tcggctcatc aacggagagt gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt
     3901 cagaaatgtt cagttcgccc aggcccgtga tcgccacgat atcgccagct tccgccagat
     3961 cggtttcgat acgttccaga ccgaggtggc ccagcacttt accgactttc gcgttacggg
     4021 ttttgccttc gctatcgatg atagtaacct gctggttcgg cttcacttta ccacgcttga
     4081 tgcggccaat gccgataacg ccaacatagc tgttgtaatc gagctgagaa atctgcatct
     4141 ggaacggacc gtcaaggtca acgtccggcg caggaacgtg gtcaacaatc gcctgataca
     4201 gcggggtcat gtcttccgcc atatcttcgt ggtccagacc cgcgataccg ttcagcgcag
     4261 aagcgtaaac gatcgggaag tccagctgct cgtcggtcgc gtcgaggtta acgaacagat
     4321 cgaatacctg atccacaacc caatcaggac gcgcgccagg gcggtcaact ttgttgataa
     4381 caacaatcgg cttcaggccg taagcaaacg cttttttggt tacgaagcgc gtttgcggca
     4441 tcgggccgtc aaatgcgtca accaccagca gcactgagtc taccatggac attacacgtt
     4501 caacttcacc accgaagtcg gcgtgccccg gggtatcaac gatgttgata cggtaatcat
     4561 tccatttgat agcggtgttt ttcgcgagga tggtaatccc acgctctttc tccaaatcgt
     4621 tggagtccat cacgcgctct tgggtttcgg cacgagagtc gaacgtaccg gattgttgga
     4681 gcagcttgtc taccagggtg gttttaccat ggtctacgtg cgcgatgatg gcgatattac
     4741 gcaatttttc gatcacaact ttgcctcagg cattagaaat agcgcgttat tgtacacgga
     4801 ttaatcgcac tacaaaacag gatcacaaac atcctccgca aacaagtatt gcagagtccc
     4861 tttgtgatcg ctttcacgga gcataaaaag ggttatccaa aggtcattgc accaatatgg
     4921 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tcacatcatg gtgcagccct
     4981 tttgcacgat gttaagcatg ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt
     5041 tcgctttatc ttttttacgg cgacacggcc aaaataattg cagatttcgt taccacgacg
     5101 acaatgacca atccaggaga gttaaagtat gtccgctgaa cacgtactga cgatgctgaa
     5161 cgagcacgaa gtgaagtttg ttgatttgcg cttcaccgat accaaaggta aagaacaaca
     5221 cgtcactatc cctgctcatc aggtgaatgc tgaattcttc gaagaaggca aaatgtttga
     5281 cggctcctcg attggcggct ggaaaggcat taacgaatcc gacatggtgc tgatgccaga
     5341 cgcatccact gcagtgattg acccgttctt cgccgactcc accctgatta tccgttgcga
     5401 catccttgaa cctggcaccc tgcaaggcta tgaccgtgac ccgcgctcta ttgcgaagcg
     5461 cgccgaagat tacctgcgtt ccactggcat tgccgacacc gtactgttcg ggccagaacc
     5521 tgaattcttc ctgttcgatg acatccgttt cggatcatct atctccggtt cccacgttgc
     5581 tatcgacgat atcgaaggcg catggaactc ctccacccaa tacgaaggtg gtaacaaagg
     5641 tcaccgtccg gcagtgaaag gcggttactt cccggttccg ccggtagact ctgctcagga
     5701 tattcgttct gaaatgtgtc tggtgatgga acagatgggt ctggtggttg aagcccatca
     5761 ccacgaagta gcgactgctg gtcagaacga agtggctacc cgcttcaata ccatgaccaa
     5821 aaaagctgac gaaattcaga tctacaaata tgtcgtgcat aacgtagcgc accgcttcgg
     5881 taaaaccgcg acctttatgc caaaaccgat gttcggtgat aacggctccg gtatgcactg
     5941 ccacatgtct ctgtctaaaa acggcgttaa cctgttcgca ggcgacaaat acgcaggtct
     6001 gtctgagcag gcgctgtact acattggcgg cgtaatcaaa cacgctaaag cgattaacgc
     6061 cctggcaaac ccgaccacca actcttataa gcgtctggtc ccgggctacg aagcaccggt
     6121 aatgctggct tactctgcgc gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc
     6181 tccgaaagca cgtcgtatcg aagtacgttt cccagatccg gcagctaacc cgtacctgtg
     6241 ctttgctgcc ctgctgatgg ccggtcttga tggtatcaag aacaagatcc atccgggcga
     6301 agccatggac aaaaacctgt atgacctgcc gccagaagaa gcgaaagaga tcccacaggt
     6361 tgcaggctct ctggaagaag cactgaacga actggatctg gaccgcgagt tcctgaaagc
     6421 cggtggcgtg ttcacggatg aagcgatcga tgcgtacatc gctctgcgtc gcgaagaaga
     6481 tgaccgcgtg cgtatgactc cgcatccggt agagtttgag ctgtactaca gcgtctaata
     6541 gttgaagttg tactacccgg cgcacaacgc cgggatttag ttgccgtgga aactttcagc
     6601 ccatctctgc atgggctttt ttctccgtca attctctgat gcttcgcgct ttttatccgt
     6661 aaaaagctat aatgcactaa aatggtgcaa cctgttcagg agactgcttt atggcaacag
     6721 gcacgcagcc cgatgctggg cagatcctca actcgctgat taacagtatt ttgttaattg
     6781 atgacaatct ggcgatccat tacgccaacc ccgccgcgca acaactgctc gcccaaagct
     6841 cccgcaaatt gtttggtacg ccgttaccgg aactgttgag ctacttctca ttaaatatcg
     6901 agctgatgca agaaagtctg gaggcggggc aaggctttac cgataacgaa gtgacgctgg
     6961 tcatcgatgg tcgctcgcat atcctttctg tgacggccca gcgtatgccg gacggcatga
     7021 tcctgctgga gatggcaccg atggataacc agcgccgctt aagccaggaa caactacaac
     7081 atgctcaaca agtagctgcc cgtgatttag tgcgcggcct ggcacatgag attaaaaatc
     7141 cgcttggcgg tttacgtggc gcggcgcagt tactcagcaa agcgttacct gacccatcac
     7201 tactcgaata taccaaagtg attatcgaac aggcggaccg gctgcgaaat ttggtcgacc
     7261 gtctgttggg gccgcagctg cccggtacgc gcgttaccga aagtattcac aaagtggctg
     7321 aacgcgtggt aacgctggtg tcgatggaac tgccggacaa cgtgcggttg attcgtgatt
     7381 acgatcccag cctgccggaa ctggcgcacg acccggatca aattgaacag gtcttgctga
     7441 atattgtgcg caatgcgcta caggcgctgg ggccggaagg cggtgaaatc attctgcgta
     7501 cccgcaccgc gtttcaactg accttacacg gcgagcgcta ccggctggcg gcgcggattg
     7561 atgtggaaga taacgggccg ggcattccgc ctcatttgca ggatacgctg ttttacccga
     7621 tggtcagcgg ccgcgaaggt ggcaccgggc ttggcttatc catcgcccgt aatttgattg
     7681 atcagcattc aggcaaaatt gaatttacca gttggccagg tcataccgag ttctcggttt
     7741 acctgcctat caggaaataa aggtgacgtt tatgcaacga gggatagtct gggtagtcga
     7801 tgacgatagt tccatccgtt gggtgcttga acgtgcgctc gctggagcgg gtttaacctg
     7861 tacgacattt gagaacggcg cggaagtact ggaggcgctg gcgagcaaaa cgccggatgt
     7921 gctgctttca gatatccgta tgccgggaat ggacgggctg gcgctgctca agcagattaa
     7981 acagcgccat ccaatgcttc cggtcatcat tatgaccgca cattccgatc tggatgctgc
     8041 cgtcagcgcc tatcaacaag gggcgtttga ttatctgccc aaaccgtttg atatcgacga
     8101 agcagtggcg ctggttgagc gcgctatcag tcattaccag gaacagcagc agccgcgtaa
     8161 tattcagctt aacggcccaa cgaccgatat catcggcgaa gcgcaagcca tgcaggacgt
     8221 gttccgtatt atcggtcggc tttcgcgttc ttctattagc gtgctgatta acggcgaatc
     8281 cggcaccggt aaagaactgg tcgctcatgc cctgcatcgc cacagtccgc gcaccaaagc
     8341 gccgtttatc gcgctgaata tggcagctat cccaaaagat ttgatcgaat cagaactgtt
     8401 tggccacgag aaaggcgcgt ttactggcgc gaataccatt cgtcaggggc gttttgaaca
     8461 ggccgatggc ggcacgttat ttctcgacga aattggtgat atgccgctgg atgtgcagac
     8521 gcgtttgctg cgcgtgctgg cagacggtca gttttatcgc gttggcggct atgcgccggt
     8581 gaaagtggat gtgcggatta tcgctgccac tcaccagaat cttgaacagc gggtgcagga
     8641 aggtaagttc cgtgaggatc tgttccaccg cctgaacgtt atccgcgttc atctgccgcc
     8701 gctgcgcgaa cgtcgggaag atattccccg tctggcgcgc cattttttac aggttgccgc
     8761 gcgcgaactg ggcgtagaag cgaagttgct gcatccggaa accgaagctg ctctgacgcg
     8821 tctggcgtgg ccaggcaacg tgcgccagct ggaaaacacc tgccgctggc taacggtgat
     8881 ggccgccggg caggaagtgt tgattcagga tttgcccggc gaactgtttg aatcaacggt
     8941 tgcggagagt acttcgcaaa tgcaaccgga cagttgggcg acacttttag cgcagtgggc
     9001 agacagagcg ctgcgttccg gtcatcaaaa tctgctttcc gaagcgcagc cagagctgga
     9061 gcggacgtta ctgacgaccg cgttgcgaca tacgcagggg cataaacagg aagcggcgcg
     9121 gctactcggc tggggccgca acactctgac gcgtaagtta aaagagctgg ggatggagtg
     9181 attcacggct tgtgtgtaaa gattgattat tgagcgcaaa ttgctggtat tttgcgcttt
     9241 actgttccga taagttcagt atgatcttgc ccggaaaacg gggagagtca ttatgctgga
     9301 atcaataatt aatctggtat cgagtggcgc agttgacagc cacacaccgc aaactgccgt
     9361 tgctgccgtg ctgtgtgccg cgatgattgg gctgtttagc tgaagctcgt tgtttatgcc
     9421 ggatgcggag aaagtcgtag gcctgataag acgcgaccag cgtcgcatca ggcatcatgc
     9481 tcagatgcct gatgcggcgt aaacgcctta tccggcctac aaaagcaata atttcaacag
     9541 gttttgttgc ccagcgcatc aggcaatctt gttctcagca cattgatggc gctttcgttt
     9601 tctactttgt aaacgaagcg ccattcactc tttaaatcac ccgtgagaac tgctgcatcc
     9661 gcgctttctg gcgcagatag gtatcaaagc acatgcaaat gttgcggatc agcaagcgac
     9721 ctttcgccgt cacctgtatt cccttctcat ccacatccac cagcccatct tttgctaacg
     9781 gggcgagcag cttgagatct tccgcaaagt aatcagcgaa gtgcaaatcc cactgtttct
     9841 caagaggggc gtaatccaga cggaagttgc agatgagcga cttaatcaca tcgcggcgaa
     9901 tacagtcatc acgcgttagc gcaataccac gccacagcgc attgccttgt tcatacactt
     9961 gctgatagta ctgcttcaac tctttctggt tctgcgcgta gcagtcgcca atcatgctga
    10021 tggcggaaac gcccatcccc agcagatcgg tatcgccctg agtggtgtag ccctggaagt
    10081 tacgatgcag cacgccttca cgctgggcca ccgccagctc gtcatcagga cgggcaaagt
    10141 gatccatacc gataaactga tagcccgatt gcgtcaggaa ggcgatggtt tcctgcagga
    10201 tatcgagttt ttgctgcgga ctcggcaggt cagcatcttt gattttgcgc tgagcagcaa
    10261 aaatggtcgg cagatgcgcg tagttaaaaa cgctcagacg atcggggttc agctccgcca
    10321 cacgtttcag ggtaaaggcg aaactctccg gcgtctgttt cggcaggccg taaatcaggt
    10381 cgatgttggt ggaggtaaaa ccaatctcac gcgcatggtt aagcagtgca aagatgaact
    10441 cttcatcctg ctcgcggtta accagacgtt gcacttcttt gttgaagtcc tgcacgccca
    10501 tgctcaggcg attaaagcct tcggcgcgta aatgatcgag tacatccagt tcgatttccc
    10561 gcggatcgac ttcgatcgaa atctccgcat cggtattgaa atggaagttt tcgcgcagca
    10621 gcttcatcag acggctgatt tgcgctttat tcagatacgt cggcgttccg ccgccccagt
    10681 gcaattggct gacgtgacgc ccggcgaaca gcggtgcacg atggacgatt tcttgctcca
    10741 gcgcgtccag atactgatcg gccttgtgct gctggcgagt aacaatctta ttgcaaccgc
    10801 agaagtaaca aagcttatgg cagaacggga tatgtacgta gagagataat ggacgctcag
    10861 gatagcgcgc cacggcttgt aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg
    10921 cggtcgggta cgaggtgtat cgtggcccgg aatagttata tttctggatc agggccagat
    10981 cccagtcgat ttgctgtaca gacatgctca ctccttccga tggcgtctct ggcggctacg
    11041 gcgtaccggc ggatgcccgt ttcgggtcat cagccggttg cgcagccact tttgtcgccg
    11101 cgacaaccgc tgtagtttaa cgaataacca caccagataa catataaccg gaagggttat
    11161 cagcagaacg ggaaggccca cggcggcaat ccgttagttg ccccgcagca gacgcatcat
    11221 gtcttcttgc ttctcgtctt cttcctcttc ttcgtcatcg tcataagaga ggccgagttt
    11281 ctgcatcaac tcatcaatac gatccagttt agcatccacc caggattgct cttcggcgct
    11341 cagggtttcg cctgcttcca gacgttccag cagcgcatcc agacactcat ccgtttccag
    11401 taattccaac tccgcctgcg gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt
    11461 ggtgactttt tcagtcactc ccaatggaat aggggtttta ctgccaatac gtggatcttt
    11521 tggtgcgttc tggcctttgc tgcctgacgt ggtgtttccg cccgctgcgc ggctgcccgg
    11581 cgcatgacca cgacgttttt tctgacgctt acggtcacga gcttcctgat ccagctcctc
    11641 gcgtgttttt cgacgcgctt ttgcatgacc tttgctgcgt gagtttgaag atgatggttt
    11701 cataatggtt cgtcttttag ccgttttatt cagtatagat ttgcggcgga atctaacaga
    11761 aagcaagcaa agaaaaaagg cgacagatta ctctgtcgcc ttttttcctg actcataacc
    11821 cttaacgggt cttacaatcc ttgcaggccg ccaacacacc ctgtttttcc attagccgga
    11881 acaatccgtg ttgattccat ttccgtttaa ttacgtctcc ggacgtttgt cttcctgaca
    11941 atcctctgtc ttcgcctcct ggcgctcctg accctcatcc tgagtcattg ttcctgttag
    12001 cgtcctcgct ctatgggtgc tactttacgc ctttgcttta aacaaacaat caaccgccat
    12061 cagtatttgc acattttcag agttttatat aaaagcaatt cactgattta tatagcttat
    12121 ttgacatgca tcgcagaatt ttctctgcta tttcgcataa agactatcgg caatgtctca
    12181 caaagcgtat ggcttttact tacaacgctc acgccgaaaa atccgccttt tattatggtt
    12241 caggtataat cgccacaaag ctttgactga cggagacgac cgctttgact aatttgaatt
    12301 atcaacagac gcattttgtg atgagtgcgc ctgatattcg ccacctacct tccgataccg
    12361 gaattgaagt ggcttttgca ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc
    12421 tgactaacca gaaaagcctg gctcgtacct caaaaacccc agggcgcacc cagcttatca
    12481 acctgtttga agtggctgac ggcaagcgtc tggttgactt gcctgggtac ggttatgcgg
    12541 aagtcccgga agagatgaag cgcaaatggc agcgtgcgct cggcgaatac ctcgaaaaac
    12601 gtcagagcct gcaaggtctg gtggtgctaa tggatattcg ccatccgctg aaagatttgg
    12661 atcagcagat gattgagtgg gcggtagaca gcaatatcgc cgttctggtg ctgctgacca
    12721 aagcggacaa actggcaagc ggcgcacgta aagcgctatt gaatatggtg cgtgaagctg
    12781 tactggcgtt taacggtgat gtgcaggttg aaacgttttc ttcgttgaag aaacaaggcg
    12841 tagacaagct gaggcagaaa ctggatacct ggtttagcga gatgcagcct gtagaagaaa
    12901 cgcaggacgg cgaataattt tcttgcctta atgcttgtgc cggatgtggc gtatccggcc
    12961 cgtaaattca atagattaca attctaccgt tggcctgata aacatagcgc atcaggcatt
    13021 acggatcttt tctttcgccc aataaaaaac gccccagtca ttactgactg gggcggctaa
    13081 aatattcagc caaatccgat tacgtgaagt aaaaggtctg aaagatagaa catcttacct
    13141 ctgtacccta cgcgactaac tttactcttt tttgttcagt tcagaaagca ctttttgtaa
    13201 ttttttttca gttgttgcat aggaatttct aatagccatc acaaaacgtg acagcttatg
    13261 ttgcttactt acgaaaaaag ggcatgttca ggcgaatctt agtgcgcctg atcccagttt
    13321 tcgccactcc ccacttccac cagcaacggc acatccagac gggtacagtt ttccatcagt
    13381 tgatgaatct gcttcgcgac ggcatcaaca tcatctttat gaacttcaaa taccagttca
    13441 tcgtgtacct gcatgatcat acgtacacgc ggttgctcag cctgtaacca cgcatcaacg
    13501 gcaatcatcg cccgtttgat aatgtcggcg gcggttccct gcattggcgc gttaatggct
    13561 gcacgttcag ccgctgcacg acgagcacca ttgctggatt tgatatccgg cagatacaga
    13621 cggcgtccgt ccagcgtttc aacgtagccc agctctttcg cctgagcacg ggtgcgttcc
    13681 atatactcca gcacgccagg gtagcgttcg aagtaaaggt ccatgtactt ctgcgcttct
    13741 ttacgtggaa tgttcaattg ccgcgccaga ccgaaagcac tcatgccata aatcagacca
    13801 aagttgatcg ctttcgcgct acggcgttgc tcgctggtga cggtttccag tggcaaacca
    13861 aacacttctg ccgccgttgc ccggtggata tcttttcctt ccgcgaatgc ggtcagcaag
    13921 cctttatcac gcgagagatg cgccataatg cgcagttcaa tctgcgagta gtccgctgag
    13981 acaatcacat aatcctctgg cgcaataaac gcctggcgga tacgacgacc ttcttcgtta
    14041 cgcaccggaa tgttttgcag gttaggatcg gttgacgata aacgtcccgt tgcagttact
    14101 gcctggtgat aagaggtatg cacacgcccg gttttcgggt tgatcatcag cggcagcttg
    14161 tcggtgtaag tcgacttcag cttcgccaga ccacgatact ccagaatcac ttttggcaac
    14221 ggatagtcca gcgccagttc ttccagtacc tcttccgacg ttgacggcgc gccacccggc
    14281 gttttcttca gcggtttaat accctgtttt tcaaacagaa tggtttgcaa ctgcttggtg
    14341 gaagaaaggt taaattcttc acctgcaatt tcatgcgctt tcttttccag ctcagccaga
    14401 cgcaaggtga gctcttcaga atgattgtgc agcactttcg gatcgatctt cacaccgtta
    14461 cgttcaatgc gtgaaagcac cggcaccagc ggcatttcga tattctcgaa gacgttcaac
    14521 ggccctttgt gtttctgcag atccggccac attttcagat gcaactgcaa ggtgacatct
    14581 gcatcttcgg cggcgtaacg tccggcctct tcgagggcaa tctggttaaa ggtcagttga
    14641 tttttgcctt tacccgcaat ctcttcaaaa gtgatggttt tgtgcttcaa ccaacgttcc
    14701 gcgaggctgt ccatatcgtg acgcccggca acgctattga gaatgtagga ctccagcatg
    14761 gtatcaaacg caatcccacg cagttcaatg ccgtaattcg ccagaatacc gcgatcgtat
    14821 ttcaggtttt gcccgacctt cagcgccttt tcatcttcca gcagcggttt tagcaactcg
    14881 agtgcacgct cgtgagagat ttgatcgggc gcatcaagat aatcatgagc aaccggaata
    14941 tatgccgcta cgcctggctc gatagcaaaa gaaagcccga ccaggttagc agagatgtta
    15001 tcaaggctgt cggtttcggt atcaaatgca aataccggcg ctttttccag cttcgcaatc
    15061 cacgctttca gtgtttcttc atcaaggata gtgacgtagt tgtcataaga aatcaccgtt
    15121 gccgtcactt ctggcgcttc gtctgcaaca ctggtttcct gaggcttcgc ggctgttttt
    15181 gcccctttgg cctgtaacca tttgcccact tcgacatcag cagtccagcg tttgaactca
    15241 tactttttga acagccccaa caactcttcc gctgccggtt gctgcacttc cagttgttca
    15301 caggtcagct ccagttcaac atcggtttta atcgtcgcca actggtatga gagataagca
    15361 acttctttgt tttgctcgag cttcgctgcc attgttttcg cgccacggaa gctcaaccca
    15421 gcaatttttt ctggctcggc atacagcgta tccagcccgc caagaccttg cagcaatgcc
    15481 tgcgcggttt tttcaccgac gcccggtacg ccaggaatgt tatcagagga gtcacccatc
    15541 agcgccagga agtcgatgat caattctggc ggcacgccgt acttattcac cacctcttcc
    15601 ggtccgagga tggtattcgt catggtattg ataagcgtaa tatttggcgt caccagctgc
    15661 gccatatctt tatcgccagt gctaatcagc accggacgcc cggctttttc ggcttcgcgc
    15721 gccagagtac cgataacgtc gtccgcttct acgccagaaa ccgccagcag cggcagtccc
    15781 atcgctttaa ccatcgcgtg cagaggttcg atttgtgctc gcagatcgtc cggcattggc
    15841 gggcgatgtg atttgtaatg ttcaaacagt tcatcacgaa aggtttttcc cttggcgtca
    15901 aagaccaccg ctgcatgcgt cggtttatat tgcatgatca gactgcgcag catgttgagg
    15961 acaccataca tcgcaccggt cggctcgcct gcgctgttag tcagcggggg aaacgcgtga
    16021 tatgcgcgat aaagataaga tgaaccatct acaaggataa gtggattttg ggggatctga
    16081 accataatgt ccgtgcctgt ttatcagatt atgggtaaag gatgccacag acaggatgaa
    16141 aacatgagtt tttcaccgct tttcgtgaaa atcttttgta gatctgctgg atcgctcgca
    16201 aatcgtcatg tggataactt tgtgcataaa ttatttacat cgcttttaat ttattatcat
    16261 gctttttaca gcatgatgtt tgtctttgtt tttcatgcag ttaaaaataa atccatccct
    16321 aataattgag ataatgggat tttttctaat gtggatagat atgaattatt tttctcctta
    16381 aggatcatcc gttatttggg tcattttctg ctaaaatctg cgccttgcgc ttctttagtg
    16441 cactgtctat ggctaacctt ttgaataaat tcattatgac gagaatactc gctgcgataa
    16501 cccttttgct gagtatcgta ttgaccattt tggtcactat tttttgttcc gtcccgatca
    16561 tcattgccgg gattgtaaaa ctattgctac ctgtgccagt catttggcga aaggtttcgc
    16621 gtttttgtga ttttatgatg tattgctggt gtgaaggtct ggcggtatta ctgcatctga
    16681 acccacacct gcaatgggaa gttcacgggc tggaagggtt aagtaagaag aactggtatc
    16741 tgcttatttg taaccaccgt agttgggcag atattgtcgt actgtgcgtg ctgtttcgta
    16801 agcacattcc gatgaataaa tatttcctca agcaacagct ggcctgggtg ccatttcttg
    16861 gcctggcgtg ctgggcgctg gatatgccgt ttatgaagcg ttattcccgc gcttatttat
    16921 tacgtcatcc ggagcgacgt ggtcaagatg ttgaaaccac tcggcgttct tgtgaaaagt
    16981 ttcgcctgca tcccaccacc attgtcaatt tcgttgaagg ctcccgtttc acgcaagaaa
    17041 aacatcagca aacccactcc acttttcaaa acttgttgcc accaaaggcg gcaggcattg
    17101 cgatggcgct aaatgtactg ggtaaacaat tcgataaact gcttaatgtt acgctgtgtt
    17161 atccggacaa taaccgtcag ccgttcttcg atatgttaag cggtaaactg acgcggattg
    17221 tcgtacatgt ggatttacag cctattgccg atgagttaca tggcgactac atcaatgaca
    17281 aaagctttaa gcgccatttt cagcaatggc tgaactcatt gtggcaggaa aaagaccggc
    17341 ttctgacgtc tctcatgtca tcacagcgtc agaataagta agttttactc gcctctgaat
    17401 gagcagaggc gagtgagtat tttaatgaac aaaacgtccg gcacgagaca taaattcttc
    17461 ttcgctcatc tcctgtccgt taaaaacaac tttacccggt gtataacgca gctgcagcga
    17521 ggcggtattg ttgtcaatcg tgattaactg aaacatctga cctaatgtca tcatcccgct
    17581 gatttgttta tcagcctgct tttgcgcttt ttccgcatcc attccttcag ataaattaag
    17641 ctgttttgcg gtttctgtga cgacattaag cggtaacttc acatcaaaat tgagcgattt
    17701 gatatctttg tttgtggatg atgaagattt ggctgggtcg gcaatactga tatccagatt
    17761 ggcatttagt tcgccgagtg cgttcttcca tcttaccggt tgtttaatgg tcggctcact
    17821 tttttgtaat aacggtgaat actctttgaa caatgcagca ttcacttctt gcagagcaac
    17881 ttcatcgttt gctaactcag ggtgtgcagc aagctgcttc tgcatcgcaa tgttatactg
    17941 gatgataaat tggcgcactg ctgagggatc aatagattca gcaatcaatg accactcacc
    18001 actaccgagt tgctgattcc cttttgttaa cttatcaagt tcgtaggtca gattgacatt
    18061 gatataatct ttgtctttaa cgcgatccag cctggttttt gcaacgaaag catcgatttg
    18121 ggcaacgtct tccccgtgat tgataatgtt aatctgatta aatttcgact cgctttcacc
    18181 aaccggaaat ttcttttctt ctaatcgcgc cagagaatca atggtaaaac tttttgccgt
    18241 tactttgttc attggtgata attgcagaac aatatcttcg actttgcctt caatatccag
    18301 gttagacaat gaactaccat caccattaaa agtaaactgg ccctcaccaa aagaaaattt
    18361 tccatactct gcaggggcaa catctaatgt cgtagagaat gagccaccaa acgcgaatcg
    18421 caatgtcgct tccacgggcg atttattttt cgcggcgata aatagcggtt gtgtcagttc
    18481 attattcact aagttcactt ttgccgctgc cagtgctggg atgacattac catgcattaa
    18541 cattgtgatg ggcaacggac cgtgctcaac atccacatca aatacaactt tctggcctgg
    18601 cttgatattg agatcgggtg caccgttatc gaatgtcatt tgcatttgaa aacgggaatt
    18661 aaaaaagcct ttgtcaaagt ttttataact taacgtcata tcgtaggcat gttcaccttt
    18721 ctttttcgca tcgttaaaat ctttaataaa cttttcgata ccaggctgaa tttgcgtacc
    18781 tgtgtaccat gtgccaccac cccagattac ggctaacgca acaataacac ctgtagctga
    18841 cttacgtatc atataaatat tccctgataa aacagcaaaa ctttgaatat ccacttatgc
    18901 tgaaataatg tccagcggca ggatgcatta tcagaatgta tttatattta ttaaataata
    18961 aaaaaagccc gtgaatattc acgtgcttta tgtaatttac attgaattat ttttcctcgg
    19021 acagatattt caccgtatca gcatactgct gaacaaaaac atccatattg ctggtatcca
    19081 taccctgcgg attcagctga tatttaccgt taacaaacat cgccggaaca ccacgcaatt
    19141 gcacatcagc tgcggctttt tcctgctgag cgaccagaga tttcaccacg aagctgttcc
    19201 acgccgcgtc gtactcttca cctttaatac ctgcattgat aaatacatcg cggatatcag
    19261 atgctgaacg aatggtctgg gttttctgta cgccttcaaa cagcggaaca gtcactttgt
    19321 cttccacgcc cagcgccatc gccacagccc atgcctgagt cagatctttg cccaggtcac
    19381 cacccatgaa gttgacgtgg tatttagtca tcttcacgcc ttccggcagt tttttcttca
    19441 cattatcaga aatatgcaga acttcttcaa actgatagca gtgcgggcag aagaaagaga
    19501 aaaactccag cacttgcggc gcgccagcta ccggtttttc cagggtagtg tactgtttac
    19561 catcttcata ctgcgccgcc gatgcgctaa acgctaaaac taaaccagcc agcgccagcc
    19621 aaatcttttt catgatctac tctctccgat taatacatag gtgttaattg caaagggggt
    19681 tcttgtagaa cttttgcctg ttctataaaa gtcgccgtct gtcgtagcca gtaatcttcc
    19741 ccggttaacc acgggaaatt tttcgggaac gcgggatcag cccaatgccg cattagccag
    19801 gcaagataat aaaccaaacg catggcgcgt aaaggttcaa tcagtccgat ttcagcggtg
    19861 tcgaactcgc taaattcttc ataagcttca ataatagttt ccagttgcat ccgctgctgg
    19921 gctttatcgc cattgagcaa catccacaaa tcctgaatag ctggaccatt acgtgcatca
    19981 tccagatcaa caaacattgg accatcgcgc cagagaatat tcccggcgtg gcagtctcca
    20041 tgtagccgca gaacggtgaa atcttcccgc cagtgtgctg taacggcggc gatcagctca
    20101 tctgtcgctt tcagaaatgc cgctttcaac ccggaaggta tcagtgtagc gtcctcaaac
    20161 agcttgcgag gctcaatgag atattcattc agaccgatgg tcgggcgatg aataaaaagc
    20221 tgtttgcgcc ccgtctggtg catacgccct aaataacgcc caaccgcttc catctgatcg
    20281 atattatcag cttcgaactg gcgaccaccg acgcttggaa aaacagcgaa ataaaatccc
    20341 tgatgattca ataaagtctg accgttaaag gccacaggtg ctgcgaccgg aacttcatca
    20401 tttaccagct gcaacgcaaa ttgatgttct tcgaggattt gatcggctgt ccaacgttca
    20461 gggcgataaa atttgacgac aaaacgtcga cgctcttcgt cctgaaattg atagacacgg
    20521 ttttcatagc tgttaagcgg ggtaagaccg gaatccaccc ggatcccttg cttaaacaga
    20581 gcgtccatga tggtatccgg gtgtagtgtc tggaaagtaa aagcgctgtt gttcatccca
    20641 tcatccggaa aacattacga atgatgaagg atatcatttc acgatgattt tggtggcgcc
    20701 gcttacaagc ttttactctt taattacgcc gcgagcgcgt aacagcgccg ttttgaagtc
    20761 ttcttcataa tctttctgca aacccgggat caccgcatct ttcgcggaat cacgcatttt
    20821 caggtgatag atcagaatgt catccgtcaa atccgccagt tcgccgtcaa aacctgactc
    20881 tttcgccagt ttctgcaaaa attgcaacaa gttaaagtct ggctcttttt gccaggctgg
    20941 ctggaggagt tcaataactt cattcagacg tttacatttc atagtaatgc tccttactct
    21001 tgagacagac acgttagcag ggtcaatccc acaataaaag aggcgatatc ggtgaatcta
    21061 atgacgacga taacaggcgt tgtgctggca ggcggtaaag ccagacgaat gggcggcgta
    21121 gataaaggat tgctggactt aaatggcaaa ccgctgtggc aacatgtcgc tgacgcgctt
    21181 aagacgcagc tctctcacgt cgtggttaat gctaatcgtc atcaggaaat ctatcaggca
    21241 ggcggtctga aagtgattga agattcactg gcggattacc caggccctct ggcaggaatg
    21301 ctttcagtaa tgcagcagga agctggtgag tggtttttgt tttgtccgtg cgatacgcct
    21361 tacattcccc atgatttagc cgcccggctt aatcatcagc gcaaagatgc gcctgtcgtg
    21421 tgggtccatg acggtgaacg cgatcacccg actattgctc tggtaaaccg cgctattgag
    21481 cctttattac tggaatatct gcaagcagga gaacgccggg taatggcatt tatgcgtctg
    21541 gctggcggtc atgcggttga tttcagcgat cataaagatg catttgttaa cgtgaatacg
    21601 ccagaggagc ttgcccgatg gcaggaaaaa cgatgatacc gttactcgcc tttgccgcgt
    21661 ggagtggtac cggaaaaact acgcttttga aaaaactgat cccggcatta tgcgccagag
    21721 ggatccgtcc ggggctgatt aagcatacgc accatgatat ggatgttgat aagccaggca
    21781 aagatagcta tgagctgcgc aaggctggcg cggtacaaac catcgttgcc agccagcaac
    21841 gatgggcctt gatgacggaa acaccagacg aagaagagct ggatctacat tttctcgcaa
    21901 gtcgaatgga tacctcaaag ctggatttga ttctggtcga agggtttaag catgaagaga
    21961 tcgcaaagat tgtgctgttt cgcgatggag ccggacatcg acctgaagaa ttagtgatag
    22021 acaggcatgt tattgctgta gccagtgatg tgccgcttaa tcttgatgtc gcattactgg
    22081 atattaatga tgttgagggg ctggctgatt ttgtggtgga gtggatgcaa aagcagaacg
    22141 gataagcaag agtaggctgg atcagacgca gccagcgtcg cacccggcat ttttttcacc
    22201 aacgcaaaaa ggccatcctg acggatagcc ttt
//
LOCUS       DAJTUP010000062        22202 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTUP010000062 DAJTUP010000000
VERSION     DAJTUP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22202
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.062"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..529)
                     /locus_tag="OO468_002476"
     CDS             complement(<1..529)
                     /locus_tag="OO468_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6259556.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPS"
     gene            537..1037
                     /gene="tnpA"
                     /locus_tag="OO468_002477"
     CDS             537..1037
                     /gene="tnpA"
                     /locus_tag="OO468_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR6259557.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(1076..1423)
                     /gene="grxD"
                     /locus_tag="OO468_002478"
     CDS             complement(1076..1423)
                     /gene="grxD"
                     /locus_tag="OO468_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR6259558.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1758..2585
                     /gene="mepH"
                     /locus_tag="OO468_002479"
     CDS             1758..2585
                     /gene="mepH"
                     /locus_tag="OO468_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR6259559.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2713..3294
                     /gene="sodB"
                     /locus_tag="OO468_002480"
     CDS             2713..3294
                     /gene="sodB"
                     /locus_tag="OO468_002480"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR6259560.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3440..4609)
                     /locus_tag="OO468_002481"
     CDS             complement(3440..4609)
                     /locus_tag="OO468_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259561.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(4775..4864)
                     /gene="ynhF"
                     /locus_tag="OO468_002482"
     CDS             complement(4775..4864)
                     /gene="ynhF"
                     /locus_tag="OO468_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR6259562.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            5163..6188
                     /gene="purR"
                     /locus_tag="OO468_002483"
     CDS             5163..6188
                     /gene="purR"
                     /locus_tag="OO468_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR6259563.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6185..7117)
                     /locus_tag="OO468_002484"
     CDS             complement(6185..7117)
                     /locus_tag="OO468_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6259564.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            7230..8441
                     /locus_tag="OO468_002485"
     CDS             7230..8441
                     /locus_tag="OO468_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR6259565.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8732..9880
                     /gene="cfa"
                     /locus_tag="OO468_002486"
     CDS             8732..9880
                     /gene="cfa"
                     /locus_tag="OO468_002486"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR6259566.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9920..10561)
                     /gene="ribE"
                     /locus_tag="OO468_002487"
     CDS             complement(9920..10561)
                     /gene="ribE"
                     /locus_tag="OO468_002487"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR6259567.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10776..12149
                     /gene="mdtK"
                     /locus_tag="OO468_002488"
     CDS             10776..12149
                     /gene="mdtK"
                     /locus_tag="OO468_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR6259568.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12190..13446)
                     /locus_tag="OO468_002489"
     CDS             complement(12190..13446)
                     /locus_tag="OO468_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR6259569.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13754..13830
                     /locus_tag="OO468_002490"
     tRNA            13754..13830
                     /locus_tag="OO468_002490"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13788..13790,aa:Val,seq:gac)
     gene            13835..13911
                     /locus_tag="OO468_002491"
     tRNA            13835..13911
                     /locus_tag="OO468_002491"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13869..13871,aa:Val,seq:gac)
     gene            14019..14324
                     /locus_tag="OO468_002492"
     CDS             14019..14324
                     /locus_tag="OO468_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR6259570.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14450..15872
                     /locus_tag="OO468_002493"
                     /pseudo
     CDS             14450..15872
                     /locus_tag="OO468_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(15884..16696)
                     /locus_tag="OO468_002494"
     CDS             complement(15884..16696)
                     /locus_tag="OO468_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259571.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16700..17485)
                     /locus_tag="OO468_002495"
     CDS             complement(16700..17485)
                     /locus_tag="OO468_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR6259572.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(17482..18150)
                     /locus_tag="OO468_002496"
     CDS             complement(17482..18150)
                     /locus_tag="OO468_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR6259573.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(18214..18852)
                     /locus_tag="OO468_002497"
     CDS             complement(18214..18852)
                     /locus_tag="OO468_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR6259574.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(18865..20966)
                     /locus_tag="OO468_002498"
                     /pseudo
     CDS             complement(18865..20966)
                     /locus_tag="OO468_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(20987..21613)
                     /locus_tag="OO468_002499"
     CDS             complement(20987..21613)
                     /locus_tag="OO468_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR6259575.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(22069..>22202)
                     /gene="fumD"
                     /locus_tag="OO468_002500"
     CDS             complement(22069..>22202)
                     /gene="fumD"
                     /locus_tag="OO468_002500"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR6259576.1"
                     /translation="DLGQEPKHIVIAGVLRTALANKRIQRSELEKQAMETVINALVK"
BASE COUNT         5547 a         5742 c         5173 g         5740 t
ORIGIN      
        1 ctgaagggtg aaccggagtt gatacttcac tttctgtctg aatactgatg taccacgtac
       61 cgcaggactg gctgacagtg acatttttca caactcccgt gacctgacgg ctattccggt
      121 agcgcatcca gccaagtttc ggcagaaaaa tacggctgtt ttcctgatcg agcttaacgt
      181 cctgcgggta gcggaatgca gcattctgtc cccgcttttt gaatcgggga aaagcagccc
      241 gcttctggaa gaagtttttg taagcccgct caaggtcttt cagtgactgt tgcaatggct
      301 gtgagggcga atctttaagc cattgcgttt cagtggcgtt tttccactca accagccagg
      361 aagccatttt cccgtaaggg atgtatttat taccggcctc atgattctca ttctgaagcg
      421 ccagagcacg attgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt tcacgctcct
      481 gttgaccacc gggtcttaac tggaatttaa atgcctgtaa tcgtttcaca ggtcaaatga
      541 tactttggtc tgtgaaaaaa gaaaccgata tccggcgtgg aaggcattgt gttttcctga
      601 tgcatgttca cctggtcttt gtcaccaaat accgacgcca gatttttgac catgacacga
      661 cagaaaaact acgcacttac ttttcaaatg tatgtgctga ttttgcagct gaactggttg
      721 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
      781 tatccagtct ggtaaacagc ctcaaaggca tatcgaatag gttactgcga cgagatcgac
      841 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      901 gtagctgcga aggtgcgcca atatccgtca tccgccagta cattgaacaa cagcaaacac
      961 ctggtcaggt ggaaaaccgc gccttatatc cccgccctga aggacgaggt tttacggcgc
     1021 accggataaa ggactgacgg atgcccgttc gcatccgtca gtattgcagg acggattatt
     1081 ccgcgtccgg ctcttcagac ttgtatttag cggcagtttc tttgatcagc tgctgcagtt
     1141 cgccacgctg atacatttcg atcacgatat cacaaccgcc gaccagctcg ccgtcaaccc
     1201 acagttgcgg gaaggtcggc cagttagcat atttcggcag ttccgcacga atgtccggat
     1261 tctgcagaat atcaacatag gcaaaacgtt cgccacatgc ggcaagcgcc tggactgcct
     1321 gggcagagaa accgcagctc ggcagtttcg gtgaaccttt catgtacagc aggatcgggt
     1381 tttcagcaat ctggcgttgg attttttcga tagtggtgct cattgtcttg cttcctcaaa
     1441 cttctttacg gcagtaatct gacattgtag cgggtcagtg cggcatcgga aaataacatt
     1501 ttcatcacgc ttttgctatt ttatcccttt gctctatttg ttgcatttca aatattcgtt
     1561 tcttctctga tgcgcggcaa actggcgtat ttataaccat ttttagcttt cactgctgca
     1621 tttttttgcg ctcgccaacg aaacgtattt tttaacaata aaagctatta actttctctt
     1681 cttctatgca ttagaatcat caagttttgt aaatcagacg caggcatgat agacctgcct
     1741 taacagaggg acgctcagtg gcgcggataa accgtatttc gatcacgctc tgtgctttac
     1801 tttttaccac cctgccttta acgcctatgg cccatgcttc aaagcaagcc agggagagtt
     1861 ctgctaccac tcatatcacc aaaaaagcag ataaaaagaa aagcacggca accaccaaaa
     1921 agacccagaa agccagccag aaaacagcga aaaaagccgc cagtaaaagt atgaccaaaa
     1981 gcaaaaccgc ttcttctgtt aaaaaatctt ccattaccgc ttctaaaaac gccaaaactc
     2041 gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag tgtaccaaac
     2101 gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga acccttgccg
     2161 acgcgcacaa agcgaaggtg caaaaagcca cgaaagtggc aatgaataaa ctgatgcagc
     2221 aaattggtaa gccatatcgt tggggcggca gctcaccgcg taccggtttt gattgcagcg
     2281 gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt acggcgaatg
     2341 aaatgtatca cctgcgtgat gcaggaccta tcgaacgtag tgagctgaaa aacggcgacc
     2401 tggtcttttt ccgtactcag ggacgcggta cagccgatca tgtcggcgtg tatgtcggca
     2461 acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact tctctcagtg
     2521 aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca aaaacacttc
     2581 gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt ctcacctttt
     2641 aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa ttaataataa
     2701 aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga tgctctggca
     2761 ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca gacttatgtc
     2821 actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact ggaagagatt
     2881 attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa ccatactttc
     2941 tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt cgctgaagct
     3001 atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga tgcagcgatc
     3061 aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa actggctatc
     3121 gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct gctgaccgtt
     3181 gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg ctatctggag
     3241 cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc ataataactg
     3301 atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg atgaggcgtt
     3361 aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca gaaggttatc
     3421 cttctgcgtt tttgtttaat tagctgttag caacgcaaac tgtttcaggt tgttttctgg
     3481 ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccgtc accggcacaa
     3541 agctgtatcc cagcccagcg gaaattaccg caccaccagc agctgctccc agcgcatttc
     3601 caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca ctggcgacat
     3661 gcatcacgcg catctgtaac ggaggtacaa ccgcaaaggt tgctgcgccc cacaccacca
     3721 tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg gattgccagc ataatcacca
     3781 tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc agtttgccgc
     3841 cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc gtgacgaaca
     3901 ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag agggtaaaca
     3961 ttgcaccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga cgcattaata
     4021 ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt aatgagaaga
     4081 acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac atccgccagc
     4141 cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc agggttaacc
     4201 ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg cttgcggcca
     4261 cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaata cgtgaaagca
     4321 tcagggtcat ataatccggc gcgatggcgg aaagaacgtt gcctagcgtg aaaattgcca
     4381 tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc atcagcggcg
     4441 cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg ggaatcgaga
     4501 catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc gttgtcccga
     4561 taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg ccttatctcc
     4621 acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca gcaatcacaa
     4681 aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa acaggaagga
     4741 gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca gtcaaaccca
     4801 cggctacgat caaaccgagg acgataaccg ttgttaccag tgaaaattta aggtcggtgc
     4861 tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca tttttacaca
     4921 ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac ttcccctttt
     4981 cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat tgaccccttc
     5041 ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg taaggtgtgt
     5101 aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg gagtgaaatg
     5161 gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca actgtgtcac
     5221 acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg tgggcagcga
     5281 ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt aaccacacca
     5341 agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag atcattgaag
     5401 cagttgaaaa aaattgcttc cagaaaggtt acaccctgat tctgggtaat gcgtggaaca
     5461 atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc gatggtctgc
     5521 tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag tatcgccata
     5581 tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat gcggtcattg
     5641 ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc ggtcaccgcg
     5701 aaatcggcgt catccccggc ccgctggaac gtaacaccgg cgcaggccgc cttgccggtt
     5761 ttatgaaggc gatggaagaa gcgatgatca aggtgccgga aagctggatt gtgcagggtg
     5821 actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag ccgcatcgcc
     5881 ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt gctgctgatg
     5941 aaatgggcct gcgcgtcccg caggatgttt cgctgatcgg ttatgataac gtgcgcaacg
     6001 cgcgctattt tacgccggcg ctgaccacga tccatcagcc aaaagattcg ctgggtgaaa
     6061 cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg cagtccattg
     6121 aagtgcatcc gcgtttgatt gaacgccgct ccgtggctga cggcccgttc cgcgactatc
     6181 gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc ttattcagcg
     6241 tctcactatc gctgagatac tcaagcagcc aggttaacgc aggcgacata tcattttgct
     6301 gccatgtcag acaacatgcc gaatccggga aggggttttc cagttcaagt gctacccact
     6361 tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg cataatcctg
     6421 ccgagataca ggttgccgat gattcccagt caggtacgac aagtcttttt tggttatcca
     6481 gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc aacgacggcc
     6541 agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg tggtgactgg
     6601 caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga ccgcctaccg
     6661 gaatcgcgcg tgttgcgcca attgccagtt ccacgcgccc gtcggaaagc gcatcccaga
     6721 caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg cgataaaaat
     6781 caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg atagctaact
     6841 gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc atttttttga
     6901 caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc acatcacggt
     6961 gccgtcgttc aaagagcggt accgccagcc actcttccag ctgacgcacg gtatagctga
     7021 ccgcagaagg aacacgatgc agctcctgtg ccgcagcgct aaaactacca ttacgcgcta
     7081 ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa aatttttgaa
     7141 accagtcatc aaatattacc gtttcacaac actaatttca ctccctacac tttgcggcgg
     7201 tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt ttagtctggc
     7261 tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct gctttcgccg
     7321 ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt agtctgttcc
     7381 ttgccggttt tgccgcagcc cagcttctgt gggggccact ctccgaccgt tatggtcgta
     7441 aaccggtgtt attaatcggc ctgacaattt ttgcgttagg tagtctgggg atgctgtggg
     7501 tagaaaacgc cgccacgctg ctggtattgc gttttgtaca ggctgtgggg gtctgcgccg
     7561 cggcggttat ctggcaagca ttagtgacag attattatcc ttcacagaaa gtcaaccgta
     7621 tttttgcgac catcatgccg ctggtgggtc tgtctccggc actggctccc ctgttaggaa
     7681 gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt gccattaccg
     7741 tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt aacaatagtc
     7801 aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc aacgtgctga
     7861 tatacgcagc ctgttcagcc agtttttttg catggctgac cggttcaccg ttcatcctta
     7921 gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa actatcgcgt
     7981 ttctgattgg tggttatggc tgtcgcgccg cactgcagaa atggcaaggc aagcagttat
     8041 taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct gcgggcttca
     8101 ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg attgccaatg
     8161 gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacat gcaaccggtc
     8221 gcgccgcagc gttgcagaac actctgcaac tgggtctgtg cttcctcgca agtctggtag
     8281 tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg atgttatcaa
     8341 cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagtt gactgcccga
     8401 atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg atatacttat
     8461 acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat actaatttcg
     8521 agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt cccgactcac
     8581 tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc ggattataga
     8641 ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg ctgttttaag
     8701 gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag aagaagtcag
     8761 tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg ccggtatagc
     8821 cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt ttaaacgcgt
     8881 tctgcaggaa ggctctttgg ggttaggcga aagttatatg gatggctggt gggaatgtga
     8941 ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc aactccccca
     9001 tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc agagtaaaaa
     9061 acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt tcagccgcat
     9121 gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata atctggaatc
     9181 tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac cagggatgcg
     9241 cgtactggat attggctgcg gctggggcgg actagcgcac tacatggcat ctaactatga
     9301 cgtaagcgtg gtcggcgtca ccatttctgc cgaacagcaa aaaatggctc aggaacgctg
     9361 taaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg accagtttga
     9421 tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg atacctattt
     9481 tgcggtggtg gatcgtaatt tgaaaccgga aggtatattc ctgctccata ctatcggttc
     9541 gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc cgaacggttg
     9601 cctgccctct gtacgccaga ttgctcaatc cagcgaaccc cactttgtga tggaagactg
     9661 gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac gattcctcgc
     9721 cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt ttacctatta
     9781 tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc aggtggtgtt
     9841 ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct atcgccccct
     9901 cttcgggggc gagttcagat caggcttctg tacctggttg attcatggca ttttctcgtg
     9961 ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatcg atttcaatgt
    10021 tgacgcgtgc gccaagtttt ttcttcccaa gagtcgtgcg ttccagtgtt tccggaatta
    10081 aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata ccgtcgatgc
    10141 caataaatcc tttgtacagg atatatttca tcaactgact atcctggact ttaaaccaga
    10201 tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc ataatatgac
    10261 ctgacattaa gtgtccgcca atttcatcac tgaatttcgc cgcacgctca acgtttaccc
    10321 aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc atcaggtcaa
    10381 aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta tgcgccacgg
    10441 aagcaccggt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca tgcgtacgaa
    10501 aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc cccgtaaaca
    10561 tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca ggtggaaaac
    10621 gcccttacca gtgaaggggt aagaatggct attttttcac tggagaatta ataaatcctc
    10681 gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag gcggcttttt
    10741 agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc agtgaagcgc
    10801 gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa actgcgatgg
    10861 gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg gcggtcgcta
    10921 tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg ctggcattaa
    10981 cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg catcaggtgc
    11041 gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg ctgtggaatg
    11101 caggttacat tatccgctac atggaaaaca tcgatccggc tctggcggac aaagccgtgg
    11161 gttatctgcg tgcgttgttg tggggcgcgc cgggatatct gttcttccag gttgcccgta
    11221 accaatgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt atcggcctgc
    11281 tggtgaacat cccggtgaac tatatcttta tttatggtca tttcggtatg cctgagctcg
    11341 gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggtcatgttc cttgccatgg
    11401 tttcttacat taaacgcgcc cgctccatgc gcgatattcg taacgaaaaa ggcaccgcta
    11461 agcccgagcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt gcgctggcac
    11521 tgttctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg ctcggtattg
    11581 ttgatgtcgc aggacaccag attgccctga actttagttc actgatgttt gtgcttccaa
    11641 tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag ggctcaacgc
    11701 tggatgcgca aaccgctgcg cggaccgggc ttatggtggg tgtctgtatg gcaaccctga
    11761 cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac gacaatcccg
    11821 aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag atttctgact
    11881 caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt tccattttct
    11941 atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt ctggcactga
    12001 ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt attatcggcc
    12061 tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt atgccgtcag
    12121 ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc acgggttgcg
    12181 cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg gctgtaattt
    12241 cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta tatccccgtt
    12301 aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg tttgcaggca
    12361 aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg ccgcaccagt
    12421 gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag tgacgctgtc
    12481 accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag taatcgttga
    12541 ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta atgcaacact
    12601 cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt ccgcgctggt
    12661 gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg taccggcatg
    12721 cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg aactgtaaag
    12781 ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag tgtcagagtc
    12841 ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg ctttcccggc
    12901 cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac ttggatacac
    12961 caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa ccaacaggcc
    13021 gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac gcaggttacc
    13081 gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt caccttcggg
    13141 atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct cggactgaac
    13201 aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac cgtactgtgt
    13261 gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga ccacggtatt
    13321 ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg tcgcgccaag
    13381 gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt tcgcatcaga
    13441 tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa gtgtaaaggc
    13501 tacggaggat ttatccacga cagatttgag atggtggcaa acaactctgt ttaaactctg
    13561 atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa atcgcccttg
    13621 caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga aatagcgcag
    13681 aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatattcg tccccgttgt
    13741 cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac atggtggggg
    13801 tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt tggttagagc
    13861 accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc agattttctt
    13921 aatctggtct tttccttttt ccctctgttt cttctctgta tccaatacgt taaaagattt
    13981 acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg ttacaacttc
    14041 attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa ccacttgctg
    14101 agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt gaaaagaacc
    14161 acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc tatctggaaa
    14221 aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa gttttcgatg
    14281 tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat ttaatcaagg
    14341 gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa tctcaaccaa
    14401 acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa tgaaaaaaat
    14461 tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc tacagcaaca
    14521 aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg ggatgccata
    14581 cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg aaataccgcc
    14641 gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc tccagcgtta
    14701 tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc tgctgggcga
    14761 atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa aatttgcagt
    14821 ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg gcgtcatgct
    14881 cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg ccacgggtca
    14941 cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt ggtcaggcct
    15001 gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct tgagcggact
    15061 ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg ataaacaaca
    15121 cgtcgttttt caccgcgata acgcaagtga aaagctaaat attacgttga tgtcgcgcac
    15181 gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct tacacatcgt
    15241 caccgatcag gcattaaatg ctgagattca aaaaggtgaa gagggccttt tggatcgggt
    15301 atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc aacgcatagc
    15361 cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc gcaaacaacg
    15421 cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata aacgagaaaa
    15481 acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac aggaaattgt
    15541 tcctcatctg aatgaacacg accataaacg gttcagtaaa ggccttgcca gggttttcat
    15601 cgataattat gcggcaatcc cttcagagtc tattcgtcga ctactggcct tacgtgaagc
    15661 ggggatctga aatccctgat gttggcgaag attatacgtt acagcaaccc gaagatattc
    15721 gtggacgcgt agcgttcggc gcgttgccct ggttgatgca cgaccagcct ttcgttcagg
    15781 gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta aagcctgcat
    15841 cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact
    15901 gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca ccttcaatgg
    15961 cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt ccccctagcc
    16021 cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca ccgctggcaa
    16081 tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt
    16141 gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa acgcgtactc
    16201 gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc gaagaattac
    16261 cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta aagccgacac
    16321 ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca gcggttgcga
    16381 ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac
    16441 tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa acttcctgat
    16501 gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga ctctccgccc
    16561 agtccgtata tccttcctca atcagtgcat gacgaatatc ggcataacta ccgccgcgtg
    16621 ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct cgcaggtctg
    16681 ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt ttaaaggttt
    16741 cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa aagaggctta
    16801 taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga aacacatctc
    16861 ccacggcttg cggatagaga cacaacagcc ccgtcaatag taacaacggc agcaatccat
    16921 acatgacacc aacataggcg acctgctgta agggattaaa tttagactgt gttgttgccg
    16981 ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt tgttttgccg
    17041 ctcgttccag ccacccctga cgacgaatgc gatagtggtg accattatcc ccaacggcat
    17101 tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa tccgcaaact tcatgcaccg
    17161 caaccagact tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc agcccacttg
    17221 ccagcaacaa tacaaagagt aacgcattcg accagtgcca taatctgacc gctttgctgt
    17281 aaagataaac cttctcacca tgatcggtgg ctgatttctt cacgccacga gcacgaagca
    17341 aggcgtgcag ccccagcacc aaccacatcc caaccagcaa tactccggca atgatcaacc
    17401 acaccggcca aaattcggga gtgaactgtg gcacatagtt cgctaactgg ctctgaaact
    17461 gttcagcatg ttgcgacggg ttcatacatt ttccttttta atcaaatgtt gaccgaaccg
    17521 gcgatacaga tgcggttttc cggcaccagg tagctgatat tgatagtatt tattatcctg
    17581 caaccacgcc tgaatttctg ggctatcttc gcgaccaaag atcaatgcgt gttccggaca
    17641 ggcgctgacg caaatgggcg gaaagccttt tgccagtcgg gactcagcgc agaaatcaca
    17701 tttatctgcc actttagtga cagggttaag gtagcgaacc aggtacggac aagcgccaat
    17761 gcaataactg cagccgatgc actgcgattt ttctacccgc acgatcccct gctcatcacg
    17821 ccacgatgcc cccgtcgggc atacgtcgat gcacggagca tcttcgcagt gctgacatga
    17881 ctggcgaaaa aagtgatact gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat
    17941 cgataaacgg cttccctgag cggggacgtg gttagtttta cggcatgcgc gggcgcaaat
    18001 attacagcca ttgcaccgtg actcgtcatg aatcatggca taccgaactt ccttttcttg
    18061 cctcgtgttc gctaacagag aagacgcaga accagtaaaa aagattactg ttcccatccc
    18121 cagaacaaat ttgcgtcgag tgaaggacat ctgattatcc cctaatgcag tggctgcaac
    18181 tgtccaccct atccatgaca tctccctgaa atttcagggg ttctgaaact gctctacctc
    18241 gaaccattcg cataagccat aagccttgcc ataggtcata aatcgctcgc taaacaagta
    18301 caacaccgcc gtcgatgact ccacctgccg tatatcggca tattcaggtg caacatccat
    18361 cgcggcgatc gcggcttcaa tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat
    18421 ggcgactttg taaggacgag gatacgtctg gcactcaaaa cgaacggcgt gtgcaatggc
    18481 gcgacaaatg tcctgctcca caacttgcaa agacatcgcc gcgtagtttt cactcatggc
    18541 ctgcgttgag taatagtaat catcctgact tcccgtcata caagcgatat ccgcgtagtc
    18601 tccgttctgc ttaatgcttt ccagcaaaac tgacagttcc tcttctgcaa ccgaataggg
    18661 tggcgagaga aacacggtgc gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa
    18721 ctcagccagg atttcagcag gttctggttc ggttaccgta tcaacggggt gacgcatccc
    18781 ctgcaaccat tgccgcttcg cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc
    18841 ctgatggttc atcttaccca ttccttacgc aggcagtaga ttgtgtgctg ccagatcggc
    18901 ggcaatatct tccagcccca gacgctgcaa tgtttcgcgg gttggacaac caagctgtgg
    18961 gtcccagccc atttctttgt agaacatcgt gagcgaagca tgcatatcgt cacgatccat
    19021 tttgtcagta ccttcagtaa agaccgggat ctgcggatcc ttgtcgaaaa cccaggaaca
    19081 gataagatcg tgttcattac gcatatcttt ggtttgcatc ggttttaccg tgtaggcacg
    19141 atgcaacgta aagatacgct ctgcagctaa atctaatttt tcctgagtca tctcttcgcc
    19201 ggtgatcgct ttgaagaatt tggcttcaag cgccagatcg ccccggtaat tacggctttt
    19261 cagcggcgaa acggtcattg gccagaccca gttgcacaga gtgacggcgt tatgcaaaca
    19321 gacccgcaac agcgaccact ttgcatattt aatttttgcg tcattgattg gcgtgtagtt
    19381 tttggtttca tcgtaagcat cttcagaacc aaaaagttct ttcgccactt cacgttgcag
    19441 tttcaatggc aagccggaac caataaagtt gatatgggta tgcgtcatgc agtcacggtt
    19501 gaacatacac ttaacaatgg aaccgacctg cgctgacgct tcattggcat ggtgaaccgg
    19561 atagccaaac ggcgaccaga gtttattttt cgcgtagccc cagtactctt tacccaaatt
    19621 ccagcgttct gcgatggcat atgaaccatc agccaggtga ctcagctcac ccacacgatg
    19681 cgccagacgg tagtaaaaat ctttaatgaa gttaacgtca cccgcttcca gttgatccca
    19741 gcgaatttct gcatactctt cagctggcag aacacgcttg aacacacctt tgctgtaaca
    19801 ataagtaaag tcgcgatgca actgcccgta gttacaccat aggccgtagt catcgaacaa
    19861 attcagacca accaggttac caatcacacg accatcgtct ttatcttcaa aatctttcgg
    19921 cccgttcggg aagatggtgg tatggacaaa gtttgctaca caagtgttac cacctgtgct
    19981 gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca
    20041 agagtggcag ccgctcattt ttactgtgta tttctctgcc gccggtccta agtcaaaaac
    20101 ggatttatag gtacgaaagc cgaccgtatt ctgattgcct ggcggaattt cacccgtttc
    20161 aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc agcgtgactt
    20221 gggatctgaa tactctgccc acgattgtgg cgtacttggc acgacatggt tgttattcgc
    20281 accaataagt tcagtcatca tgtaatcatt gagacgcttc atctcctgac gatcggcaat
    20341 gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac ccattattgc
    20401 gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga gaggaataag
    20461 gttttcccca gcctgaccaa tagccgccac acaggtttcc gggctggtca atcgacaaat
    20521 ttcttccgtc gttgcgcgcg tcccttttcc ccataagaaa tcggcttttt ccaggctaac
    20581 tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga taataatgac
    20641 gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca cccatatggg catcgcgact
    20701 aaatttcctt tggtaaaagt agaaagtgag gtgatattta cgcgagaact acagggggca
    20761 ccagatccag gtaatgggcc ggtagcaaag actaatttat tcgcttcatc gaaaggtttc
    20821 gtgcctagcg gtacttcgtc atacataatt ttgtagccga agcccatgcc accgacaaaa
    20881 cttttaaact tactggaatc ttcgagggta atatttcctg tcgtgagatt gactcttaat
    20941 atattacctg tccaaccgtt agccatgatt ttttcctttg caagattcac acagtaatat
    21001 ctttccactc gataattttt aacgcccctg ttgggcaggc gtttgcgcat tcaccgcata
    21061 acacacattt cgaggatttt ttactttcgg tatttacggt ggccatcatc cacgggcatg
    21121 ccgtagtaca ggcgctacag ccaatacaac gtttatgatc gacggtaata cagccttctt
    21181 tctgctgcca ggtaatcgca ccaatcgggc agacgttcat gcattgcggc tctttgcatt
    21241 gacggcaggt gtccgcggta tagttgagat cgccatacag gccgccgcca gagccaaccc
    21301 cgttgtcgcc aaagaaataa ttgcgatgga ttttgatacg ggagaagaat gtccctactg
    21361 agccatcgtt gaagttggta caagagattt cacagcggtg gcaacccgtg catcgcgctc
    21421 gctgggtcac cagcacccct ttgggtgtat ttatcaaccc tactgtgccg ctatcaatat
    21481 cttcctgttt gcaacccaaa agtgacagta acgcaggggc aattgttaga ccagcaaggc
    21541 cttttcctga tatacgcaag aattctaatc gcgttaagcc aatatctaat agtggacgat
    21601 caaccgggtt catttatttc gcatcctctt cgcaaacaca gtgctgacaa ttcagcagcc
    21661 tgatattaag cattcaacta atacgctgct gactctgtcc ccaacaccag atattttccc
    21721 taacccgata gtgtatgaga aaagatttaa cgccgataaa ctaatccttt atggttagga
    21781 gaaaaaataa tgtgattatt ctgagccctt aacattgatc gttatcaatt aaaattacaa
    21841 agcaagaaat tataagcgaa ctgatattta ttatcatttg aaataaattt aacttaagcg
    21901 acaatcattt gtgacgttca ttgcagaaaa taaaaataac attatcgcta taaattaaaa
    21961 tacataccga aattcgtata atttcagcag ttgttattta gaatgattaa ctggaccgca
    22021 actgaagcaa gaatatcagg atgaagctta ccgcctcatc ctgaatggtc attttaccag
    22081 tgcgttaatg acggtttcca tcgcctgctt ttccagctca ctgcgctgaa tgcgtttatt
    22141 ggctaatgct gtacgtaata cgcctgcgat aacaatatgc tttggttcct gccccagatc
    22201 gc
//
LOCUS       DAJTUP010000063        21787 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTUP010000063 DAJTUP010000000
VERSION     DAJTUP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.063"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..2373
                     /locus_tag="OO468_002501"
     CDS             <1..2373
                     /locus_tag="OO468_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR6259577.1"
                     /translation="TIVDTRNVNFVEITPEKGIAACLTTESLDAMGVNTDAFPAFKQL
                     DKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNARGYVPPERWDEGINALLLG
                     YSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNSTWSRSSGQTAEWKNLSSY
                     LQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNMLPDSLKGFAPVVRGIAKS
                     NAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVEIKEADGSVNSYSVPFSSV
                     PLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGPWGTTWYGGGQYAEYYRAA
                     MFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLYAKTLNQLGTNFQLMGYRY
                     STSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFYTKRGKLQVNISQQLGEYG
                     SFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNYSKSRGQPDADQVFALNFS
                     LPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLGLTETLLDDGNLSYSVQQG
                     YNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNYALSGSLVAHSQGITLGQS
                     LGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSYRENRIALDAASLKRNVDL
                     ENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRFGAIATLDGIQTNSGIIDD
                     DGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINSAITRMDAICR"
     gene            2364..3347
                     /locus_tag="OO468_002502"
     CDS             2364..3347
                     /locus_tag="OO468_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6259578.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3445..3985
                     /locus_tag="OO468_002503"
                     /pseudo
     CDS             3445..3985
                     /locus_tag="OO468_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3993..4508
                     /locus_tag="OO468_002504"
     CDS             3993..4508
                     /locus_tag="OO468_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6259579.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4474..5211
                     /locus_tag="OO468_002505"
     CDS             4474..5211
                     /locus_tag="OO468_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR6259580.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5322..6332
                     /gene="pyrD"
                     /locus_tag="OO468_002506"
     CDS             5322..6332
                     /gene="pyrD"
                     /locus_tag="OO468_002506"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR6259581.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6506..7048
                     /gene="zapC"
                     /locus_tag="OO468_002507"
     CDS             6506..7048
                     /gene="zapC"
                     /locus_tag="OO468_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR6259582.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7045..8154)
                     /gene="ycbX"
                     /locus_tag="OO468_002508"
     CDS             complement(7045..8154)
                     /gene="ycbX"
                     /locus_tag="OO468_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR6259583.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8398..10506
                     /gene="rlmKL"
                     /locus_tag="OO468_002509"
     CDS             8398..10506
                     /gene="rlmKL"
                     /locus_tag="OO468_002509"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR6259584.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10518..12425
                     /locus_tag="OO468_002510"
     CDS             10518..12425
                     /locus_tag="OO468_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6259585.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12555..13808
                     /gene="pqiA"
                     /locus_tag="OO468_002511"
     CDS             12555..13808
                     /gene="pqiA"
                     /locus_tag="OO468_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR6259586.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            13813..15453
                     /gene="pqiB"
                     /locus_tag="OO468_002512"
     CDS             13813..15453
                     /gene="pqiB"
                     /locus_tag="OO468_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR6259587.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15450..16013
                     /gene="pqiC"
                     /locus_tag="OO468_002513"
     CDS             15450..16013
                     /gene="pqiC"
                     /locus_tag="OO468_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR6259588.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16269..16436
                     /gene="rmf"
                     /locus_tag="OO468_002514"
     CDS             16269..16436
                     /gene="rmf"
                     /locus_tag="OO468_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR6259589.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16506..17024)
                     /gene="fabA"
                     /locus_tag="OO468_002515"
     CDS             complement(16506..17024)
                     /gene="fabA"
                     /locus_tag="OO468_002515"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR6259590.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17093..18853)
                     /locus_tag="OO468_002516"
     CDS             complement(17093..18853)
                     /locus_tag="OO468_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR6259591.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19038..19490
                     /gene="matP"
                     /locus_tag="OO468_002517"
     CDS             19038..19490
                     /gene="matP"
                     /locus_tag="OO468_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR6259592.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19566..20612)
                     /gene="ompA"
                     /locus_tag="OO468_002518"
     CDS             complement(19566..20612)
                     /gene="ompA"
                     /locus_tag="OO468_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR6259593.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(20968..21477)
                     /gene="sulA"
                     /locus_tag="OO468_002519"
     CDS             complement(20968..21477)
                     /gene="sulA"
                     /locus_tag="OO468_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR6259594.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            21696..>21787
                     /locus_tag="OO468_002520"
     CDS             21696..>21787
                     /locus_tag="OO468_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCR6259595.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGS"
BASE COUNT         5559 a         5127 c         5708 g         5393 t
ORIGIN      
        1 acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa ggggattgcc
       61 gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc gtttccggct
      121 tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc ggatgccagc
      181 gtaacgttta atgtgaataa actccgtctg gaaatttcag taccgcaaat cgccatcaaa
      241 agtaatgccc gtggttatgt cccccctgaa cgttgggatg aagggatcaa cgcgctatta
      301 ctgggatatt cgtttagcgg ggctaacagt attcatagca gcgcagacag tgattctggc
      361 gacagctatt ttctgaattt aaacagtggc gttaatttag gcccatggag attacgcaac
      421 aattcaacat ggagtcgcag tagtggccaa accgcagaat ggaagaatct cagcagctat
      481 ttgcagcggg cggttattcc actaaaaggc gaactgaccg taggtgatga ttatactgca
      541 ggcgattttt tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga tgacaacatg
      601 ctgccagaca gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa aagcaatgcc
      661 cagataacga ttaagcaaaa tggttacacc atttaccaaa cttatgtatc gcctggtgct
      721 tttgaaatta gtgatctcta ttccacgtcg tcgagcggtg atttgttagt tgaaatcaaa
      781 gaagcggacg gcagcgtcaa tagctacagc gtaccgtttt ccagcgtgcc attactccag
      841 cgtcaggggc gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa tagtaatgaa
      901 cagcaggaga gcaaatttgc ccagaccacg ttgcagtggg gcggaccgtg gggaacgaca
      961 tggtatggtg gtggacaata tgctgaatat taccgtgccg ccatgtttgg tctgggattt
     1021 aaccttggcg atttcggagc aatttcgttc gatgcgaccc aggcgaagag tacgctggca
     1081 gaccaaagcg aacataaagg tcagtcatat cgttttctgt atgccaaaac gctcaaccaa
     1141 ttgggcacta attttcaatt gatgggctat cgctattcga cgtcgggttt ctacaccctt
     1201 tccgacacca tgtataaaca tatggatggc tacgaattta atgacggtga tgatgaagat
     1261 acgccgatgt ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa actgcaggtc
     1321 aatatctccc agcaattagg cgagtacggt tcgttttatt taagtggtag ccagcaaact
     1381 tactggcata ccgatcaaca ggatcggcta ttacagtttg gctacaacac gcaaattaaa
     1441 gatctctcgc tgggggtttc ctggaactac agtaagtccc gtggtcaacc tgatgctgat
     1501 caggtgtttg cactaaattt ttccctgccg ctcaatctgt tgctccccag aagtaatgat
     1561 agctatacca ggaaaaaaaa ttacgcctgg atgacctcta acaccagtat cgataacgaa
     1621 gggcacatta cacaaaacct gggtttaacg gagacactac tcgatgacgg taacctgagc
     1681 tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggtag cgccagcatg
     1741 gactacaaag gggcgtttgc agatgcccga gtgggctaca actacagcga taacggcagt
     1801 caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca gggcattacc
     1861 ctggggcaat cgctggggga aactaacgtg ctgattgcag caccaggcgc agagaatact
     1921 cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt tgtcccctat
     1981 gccacttctt atcgggaaaa tcgaatcgca cttgatgcgg catcgttaaa acgtaacgtg
     2041 gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt tctggcggag
     2101 ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg tataccgctg
     2161 cgttttggcg cgatagcgac gctggacggc atacagacta atagcggcat tattgatgat
     2221 gatggctcgc tctatatgtc cggtttgccc gctcagggag cgataactgt acgctggggc
     2281 gaagctcccg atcaaatttg tcatatcagt taccagctta ccgaacaaca aattaactct
     2341 gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc gtgttattac
     2401 tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaactgct gcttatatcg
     2461 ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc gttaaaactc
     2521 tggatgaaga agaaaatacg gctggagtag ttaaagacaa attttatcag tggaacgaat
     2581 cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt ggccgatggg
     2641 cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa attaatgact
     2701 atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctactgcg gttcctttcg
     2761 aaaacgtggg cacaggggca gatacacgat ggcatatttg cgatcccggc ggtcaacgtt
     2821 taggtggcca gggggctagc ggtaatagcg gtagcttttc cctgaaaata ttgcagccgt
     2881 tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa tgctacaaca
     2941 tacccgcagg tgattcctgc acgactactg gtacaccggt tttagtgtat tacctgtctg
     3001 gtacgatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc gaggttgatc
     3061 tgggcgacgt atttgcggct aactttcgtg ttgtagggca taagcctctt ggggccagaa
     3121 cggcagaact tgcaattccg gtcaggtgta acacgggaaa cgcagggtta gttaacgtca
     3181 atctgagtct gacggcaacc tcagacccca gctatcccca ggcgattaag acgtcacgtc
     3241 ctggcgtggg cgtggtggtg accgatagcc agcacaacat tatttcccct gctggtggga
     3301 cactaccgct ctctattcct gatgatgcag acagtatccg cgaatgaatg tctatccagt
     3361 cagcacgaca agcgtaccac cagaaaccgg gcgatttgaa gccacggcaa cggtgagaat
     3421 aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatatcagtg cgttattttg
     3481 ttctttagcc ttcttaacat ccatgtcggg atggctgggc ctgaacaagt cagtatgcat
     3541 atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc attacaaaat
     3601 attcatattg gtgattttaa tatcagtgat tttcaggccg cgaataccgt aagcactgct
     3661 gctgatttga atattgatat caccggttgt gccgctggta ttactggcgc ggacgtcctt
     3721 tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga cacaggcgga
     3781 agcggtggta tggcaacggg gattgccgtg cattcttgat gcgcaaagtc agcaagaaat
     3841 cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca cactcaaata
     3901 tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga cggcggttct
     3961 ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc tggattctgt
     4021 tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg gtcaacatta
     4081 gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa atcaatgtgc
     4141 agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg gagaaatctt
     4201 tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgatgtc accttttcag
     4261 gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg gatgcagcca
     4321 gtgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt aaccaggcga
     4381 gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg caattgcgac
     4441 ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt gtacttcatt
     4501 atgactaata cgtggaacag attagcgctc ttgattttcg ccgttttatc gctgctggtg
     4561 gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc gatttatctt tccggcagac
     4621 agagaatcga tatctatttt actgactaat accagtcagg aatcttggct tattaacagc
     4681 aaaatcaatc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc agcactatta
     4741 ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt gcgcttgctg
     4801 agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt aagcattgcc
     4861 agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg ctcggtattt
     4921 aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta tcaacaatta
     4981 cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta ctacattaac
     5041 ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagtcgt gccgcctaaa
     5101 agccagcgtc agacaaactg gtgtcaggta atagcaccgt gtcatgtcgc ctggcggacg
     5161 ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg aaacaggttc
     5221 tgaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga actcgcacat
     5281 aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac cccttcgttc
     5341 gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt cagcaattac
     5401 gccgcattac cggaacaccg tttgaagcac tggtgcggca gaaagtgcct gcgaaacctg
     5461 ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt cttgataaag
     5521 acggggagtg cattgatgcg ttaggcgcga tgggatttgg atcgatcgag atcggtaccg
     5581 tcacgccacg tccacagcct ggtaatgaca agccgcgtct ctttcgtctg gtagatgccg
     5641 aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc gtagagaacg
     5701 taaaaaaggc ccattatgac ggcgtcttgg gtattaatat cggcaaaaat aaagatacgc
     5761 cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat gcctatgcgg
     5821 gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg ctgcaatatg
     5881 gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat ttgcaagcga
     5941 tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct gaagacgaat
     6001 tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt gcaaccaata
     6061 ccactctcga tcgttctctg gttcagggaa tgaaaaattg cgatcaaacc ggtggcttaa
     6121 gtggtcgtcc gcttcagtta aaaagcacag aaattattcg ccgcttgtca ctagaattaa
     6181 acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct gcgcgtgaaa
     6241 agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt aaaggtccgc
     6301 cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct ctttataaga
     6361 taatcagggc tttattttca gacctggttg ttttatattc atcgttgttg cttatttaga
     6421 cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg
     6481 gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta
     6541 ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc
     6601 acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct tttgtgttga
     6661 tgatgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt tatctaaaga
     6721 ccaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgttatctca aaccgcaaat
     6781 gccgaaaagc tggcattttg tctcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc
     6841 ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg ttgaatctgg
     6901 cgaaaatgcc gcgctatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat
     6961 gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg tgaatgttga
     7021 cagcttcagc ctcgaacagg cagtctaacg cgtcagctta agggcagtct tcggtacaca
     7081 gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg gtgtgacttc
     7141 gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag aataagggat
     7201 acgaattccc tgattttcta attgctccag caacacttgt tggttatttc cacgaaatgc
     7261 ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc
     7321 atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt ccacctcatc
     7381 gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc
     7441 gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt
     7501 ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc
     7561 aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg ctgacgcgcc
     7621 ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg
     7681 gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag
     7741 aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg
     7801 cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg caattcgcgc
     7861 ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg
     7921 aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg
     7981 agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt
     8041 gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat gtgtaagacc
     8101 aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg
     8161 gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc
     8221 gcaacaattt tcttagcgcc tgtcccgtta ggctatttta tttgtcagtt tggccctggg
     8281 ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct ctccatgtcc
     8341 agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg
     8401 aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa
     8461 aatctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca
     8521 cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg
     8581 ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg
     8641 acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc
     8701 atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcaca
     8761 cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc
     8821 tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg tttacatctg
     8881 cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgctatt
     8941 gtgatgcgat ccggctggca gtcaggaaca ccgctgctcg atccgatgtg tggttccggt
     9001 acgttgctga ttgaagcggc gatgctggcg accgatcgcg caccaggctt gcaccgtggg
     9061 cgttggggct ttagcggctg ggcgcagcat gatgaagcta tctggcagga agtgaaagcg
     9121 gaagcgcaaa ctcgcgcccg taaaggccta gctgagtata gctctcattt ttacggttcg
     9181 gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt
     9241 gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg
     9301 ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag cgaaccggcg
     9361 ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg tggctggaat
     9421 ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg tgcagacaaa
     9481 cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca cgttgccgaa
     9541 agcacaccag acagtaaacc ggcaatggta gcggaagatt acgccaaccg cctgcgtaag
     9601 aacctcaaaa aattcgagaa gtgggcacgc caggaaggga ttgaatgtta ccgcctgtat
     9661 gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg ggtggtggtg
     9721 caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg tctgttcgat
     9781 attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt gctgaaaacc
     9841 cgtgaacgcc ataagggcaa aaaccagtac cagaaactgg gcgagaaggg cgagtttctt
     9901 gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct cgatactggt
     9961 ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa aggcaaagat
    10021 ttcctcaacc tgttttctta taccggcagc gccaccgtgc atgcgggatt aggcggtgca
    10081 cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg aatgggcaga acgcaacctg
    10141 cgtctgaatg gcctgactag gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg
    10201 ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac cttctctaac
    10261 tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct gatgaaagat
    10321 ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa acgtggcttc
    10381 cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa
    10441 acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca
    10501 gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg
    10561 acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg
    10621 tgggccgcaa cggcgcagga aaatcgacgt taatgaaaat cctcaaccgt gaacaagggc
    10681 tggatgacgg tcgcattatt tatgagcaag atttgattgt agctcgtctg caacaggatc
    10741 cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt gaagaacaag
    10801 cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga
    10861 aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac ctgtggcagc
    10921 tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac gttgcgctgt
    10981 cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagcaatc
    11041 cgcgcgtatt gctgctggac gaaccgacta accatctgga tatcgaaacc atcgactggc
    11101 tggaggggtt ccttaaaacc ttcaatggga cgattatttt catctcccac gaccgttcgt
    11161 ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg gtgacctatc
    11221 cagggaatta cgaccagtac ctgcaggaaa aagaagaagc cctgcgcgtg gaagaactac
    11281 aaaatgccga gttcgatctc aaactggcgc aggaagaagt gtggattcgc caggggatca
    11341 aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcgatg cgtcgcgaac
    11401 gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct
    11461 ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttaacggt aagcaactgg
    11521 tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg
    11581 ggtgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaagcg gacagcgggc
    11641 gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc gcggaactgg
    11701 atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg atggttaatg
    11761 gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg aaacgggcga
    11821 tgacgccagt acgtgcgttt tctggcggtg agcggaaccg cttgctgctg gcgcgtttgt
    11881 tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt gatgtcgaaa
    11941 cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg ctggttagcc
    12001 acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa ggcggcggta
    12061 aaattggtcg ttatgttggc ggttatcatg atgcccgtgg tcagcaagaa caatatgtgg
    12121 cactcaaaca gcctgcggtg aaaaaaagcg aagaagccgc cgcgccaaaa gcagaaactg
    12181 taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc
    12241 aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaagtg gcggatgctt
    12301 ccttcttcag tcagccgcat gagcagacgc aaaaagtgct tgctgatatg gctgctgcag
    12361 agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg
    12421 gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg
    12481 aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa atagcgcatc
    12541 attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt
    12601 gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca tgtccccggt
    12661 gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc gcctatgcgt
    12721 tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg
    12781 caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact
    12841 acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga
    12901 taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa gagcaactgg
    12961 cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc gcgggtgtgc
    13021 tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc agctttctcc
    13081 cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat cgtcgctggt
    13141 tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca ggcgtcacgg
    13201 ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct gctgatgaac
    13261 ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc ctgcagtgga
    13321 cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca
    13381 tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg gtcattctgt
    13441 tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt attatggtgc
    13501 caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg catggcaagc
    13561 gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga
    13621 tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga
    13681 gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt
    13741 ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg
    13801 agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg
    13861 tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat
    13921 cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga aggaattgaa
    13981 ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca
    14041 ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg tatggaaaaa
    14101 ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg cgaagggatt
    14161 agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg cgcgaaaggc
    14221 agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc tgatgcgaaa
    14281 ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatccggtg
    14341 ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac gcaaaaacgc
    14401 aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac cagcaatgtt
    14461 cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat gcgtgtggag
    14521 atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc ggaaggtctg
    14581 gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc
    14641 attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta
    14701 cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg taccgtaagc
    14761 aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg
    14821 gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt
    14881 gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac
    14941 ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc tgcaataacc
    15001 ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg cttggcgcaa
    15061 atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg
    15121 attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg
    15181 acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt gccgacggat
    15241 atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca
    15301 gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa
    15361 ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa
    15421 aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcagcac
    15481 tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg
    15541 tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg gtagagcagg
    15601 tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt
    15661 atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg cgcaacaccc
    15721 tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag cctctgggaa
    15781 gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat gatggcaagg
    15841 tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc
    15901 gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagtg ctggccggtg
    15961 tctggagtca ggaagccgct tctattgcac aagagataaa gcgtctacct taattataaa
    16021 gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaacgt
    16081 gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt gtagctcaca
    16141 aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta
    16201 actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga
    16261 aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc
    16321 aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg agtcaaaggt
    16381 cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct
    16441 gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat
    16501 gataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct ggcggtatag
    16561 atcagacgac aatcaaccag cacttcgcca tctgccagac ccataatcag acgacgatta
    16621 acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgacca
    16681 gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc cagccagccg
    16741 aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga
    16801 tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca
    16861 acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtctatcatc
    16921 agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gtgaccagag
    16981 gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt
    17041 attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg
    17101 gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct tcctgttgcg
    17161 atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc
    17221 acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga
    17281 atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg cgaacgttag
    17341 cagtggggat aatgacacct tgtttccccg ttaactcacg ttgctggcaa atagcaaaga
    17401 agccttcgat tttctcattt aaaccaccga ccggctggac gcggccgaac tgatcgactg
    17461 aacctgtgat agcgatactc tgattcactg gcacatcagc gagggcgctt atcagggcgc
    17521 agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc tcaaatgtca
    17581 gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcctgca
    17641 tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg
    17701 tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc
    17761 gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt
    17821 cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca gcgaggaaac
    17881 cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc
    17941 catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa
    18001 gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc caagcatccg
    18061 ctcccggggc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga caccactggc
    18121 ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct
    18181 ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa
    18241 tgactttcag cttcaatggc attgaaggca cagagacggg gagagggcgc gactcatcaa
    18301 acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag
    18361 gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca
    18421 gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgctccg
    18481 cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca
    18541 ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgatcgccc accagttggc
    18601 ctgcatcgct ttgtagcgta cgcgcggcat cggcaataag attcagatac tcagactctt
    18661 ccggggcctt agccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagcg
    18721 caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg
    18781 gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt
    18841 tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac
    18901 cggcactaca gcacacgtag aaagcaccga caatactcct ggctgggcgt taaagctcac
    18961 aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa gagttacacg
    19021 gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc ggttggaaat
    19081 ggaagtatct ggtgaagaag catcgcgaag gggagttaat tacccgttac atagaagcca
    19141 gtgccgccca ggaagccgtc gatgtgttgc tctcgctcga aaatgaaccc gtgctggtaa
    19201 atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag cagaccattc
    19261 gggccagacg gaagcgccat tttaatgcgg aacaccagca tacgcgcaaa aaatcgatcg
    19321 atctggaatt tattgtctgg caacgtctgg ctggtcttgc acagcgtcgc ggtaaaacgc
    19381 tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa tacgcgaata
    19441 aaatgtcttc tcttaagcag gatctgcagg cattgctggg taaggaataa ctgacgaaag
    19501 tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt ctaccagacg
    19561 ataacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc gatctctacg
    19621 cgacgatccg gagccaagca gtcgatcagt gcagcacgct gtttcacgtt gtcacaggtg
    19681 ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc tgccgggata
    19741 cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag accctggttg
    19801 taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc gtctttcgga
    19861 tctaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc cggtttcagg
    19921 gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt ggtctgtact
    19981 tccggtgccg gagctggagc aactaccgga gctgcttcgc cctgaccgaa acggtaggaa
    20041 acacccaggc tcagcagacc gttgtccgga cgagtaccga tggtgtttgc gtcaccgatg
    20101 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    20161 ccacctgcga aaaccggaga aacgccagtg tcgtggtctt taaaggatgc gccaccaggt
    20221 acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta gatgtccaga
    20281 tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc tttgtatgcg
    20341 ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc catttcaaaa
    20401 ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg gttttcgtgg
    20461 gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc cagtttagca
    20521 ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa accagccagt
    20581 gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc atccaaaata
    20641 cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg atctgctcaa
    20701 tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt aaagtctaca
    20761 acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa agtcttgtat
    20821 aaggtatgtt taatcttttt gtcagcgaca atttacagaa aaaaaacgcg gaaaccgctt
    20881 cagacaagcc ttcgcaagga aaattagtca cgactgaaag cattggctgg gcgacaaaaa
    20941 aagttctagg attaatccta aatttactta atgatacaaa ttagagtgaa tttttagccc
    21001 ggaaagttgt ctcgttgtgt gagaggatgc gcttaccgga cgcataataa accccatagc
    21061 gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca aatcatctgt
    21121 caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca ttgactccac
    21181 tgtgtggcaa ggggagagct ggctaatctg cattactttc gttaagggta gcccagatgc
    21241 ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc agcgcgattg
    21301 ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg gctgatcttc
    21361 gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga cacgcgcaat
    21421 tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag tgtacataat
    21481 caatccagcc cctgtgagtt actgtatgga tgtgcagtac atccagtgac aacaaagatc
    21541 aaccctattt tcggaaagag cctcgcaaat tttgtcgttg gtgacgggaa aacataaatt
    21601 aatcttgccc cttaagaata agttgcctat tttcgtagtt aacggatccg ttaatgtgaa
    21661 tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc tataagcgga
    21721 tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga tcattgtttg
    21781 gcagtta
//
LOCUS       DAJTUP010000064        21519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTUP010000064 DAJTUP010000000
VERSION     DAJTUP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.064"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(23..1021)
                     /gene="fbp"
                     /locus_tag="OO468_002521"
     CDS             complement(23..1021)
                     /gene="fbp"
                     /locus_tag="OO468_002521"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR6259596.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1197..2570
                     /gene="mpl"
                     /locus_tag="OO468_002522"
     CDS             1197..2570
                     /gene="mpl"
                     /locus_tag="OO468_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR6259597.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2653..3204)
                     /locus_tag="OO468_002523"
     CDS             complement(2653..3204)
                     /locus_tag="OO468_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR6259598.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3298..4650
                     /gene="pmbA"
                     /locus_tag="OO468_002524"
     CDS             3298..4650
                     /gene="pmbA"
                     /locus_tag="OO468_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR6259599.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4833..5215
                     /gene="cybC"
                     /locus_tag="OO468_002525"
                     /pseudo
     CDS             4833..5215
                     /gene="cybC"
                     /locus_tag="OO468_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO468_002526"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="OO468_002526"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR6259600.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO468_002527"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="OO468_002527"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR6259601.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO468_002528"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="OO468_002528"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR6259602.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO468_002529"
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="OO468_002529"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR6259603.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO468_002530"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="OO468_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR6259604.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="OO468_002531"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="OO468_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR6259605.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="OO468_002532"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="OO468_002532"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR6259606.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO468_002533"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="OO468_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR6259607.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO468_002534"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="OO468_002534"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR6259608.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO468_002535"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="OO468_002535"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR6259609.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO468_002536"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="OO468_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR6259610.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="OO468_002537"
     CDS             complement(18174..18569)
                     /locus_tag="OO468_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6259611.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO468_002538"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="OO468_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6259612.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="OO468_002539"
     CDS             19485..20078
                     /locus_tag="OO468_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR6259613.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="OO468_002540"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="OO468_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR6259614.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20798..>21519
                     /locus_tag="OO468_002541"
     CDS             20798..>21519
                     /locus_tag="OO468_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6259615.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5198 a         5326 c         5669 g         5326 t
ORIGIN      
        1 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
       61 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
      121 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
      181 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
      241 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
      301 cgatataacg cgaggtatac gggcgattgg tggatttatc ttcttcctgg cagaatttaa
      361 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
      421 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
      481 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
      541 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
      601 ccggcgtgcc aacaggcgta acgcggcggt agatggagaa aatggtaccg acagagacgt
      661 taacatcgat gttggacgag ccatccaggg ggtccatcag caccacgtat tttgcgtgtt
      721 cacagccttc aaagacgaca atctcatctt cttcttcaga ggcaatcccc gcaacgatat
      781 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
      841 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
      901 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
      961 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     1021 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     1081 tcaatgttgc gctcaggtga atcgcgccag caaattgcgg attatcctga aatgcgtttc
     1141 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     1201 gcattcatat tttaggaatt tgtggcacat ttatgggcgg tctggcgatg ctggcgcgcc
     1261 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     1321 ttgagaagca aggcattgag ctgattcagg gttacgatgc cagccagctc gagccgcagc
     1381 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     1441 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     1501 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     1561 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     1621 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     1681 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     1741 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     1801 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     1861 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     1921 gtgagcaggg tcactggcag gcgaaaaagc tgaccaccga tgcttccgaa tgggaagttt
     1981 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     2041 acaatggcct gatggcgatt gcagcggctc gccatgttgg tgtagcgccg gcagatgccg
     2101 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     2161 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     2221 cggcgctgcg tggcaaagtt ggtggtacgg cgcgcattat tgctgtgctg gaaccgcgct
     2281 cgaataccat gaaaatgggg atctgcaaag acgatctggc accttcatta ggtcgtgctg
     2341 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     2401 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     2461 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     2521 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcttca
     2581 gcctgatata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     2641 caacaacgaa aattaccctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     2701 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagtgtacg
     2761 cagttgctga cgatcggcat cgggccacag attcaatact tcagcgatgg cgtcatcacc
     2821 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     2881 gtgacggttt ttcagcttat ccagcgcctg acgaataggc tctacgtcgc gctggcgcag
     2941 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     3001 ctcaatagcc gggcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     3061 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     3121 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     3181 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     3241 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     3301 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     3361 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     3421 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     3481 agtgatggcg cgctggggat cactgtttat caccagaacc gtaaaggtag cgcatcgtct
     3541 accgatttaa gcccgcaggc cattgcccgc accgtacagg cggcgctgga tattgcccgt
     3601 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     3661 ccgaatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     3721 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     3781 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     3841 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     3901 gagcgtgatt acgcctacac cattggccgc gcgatgagcg atctgcaaac gccagagtgg
     3961 gtcggggccg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     4021 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     4081 ggggcgatag cgggtggctc ggtttaccgt aaatctacct ttctgctgga ttcgctgggt
     4141 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     4201 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     4261 ggcatcctga ctcagtggct gctgaccagc tactcggcgc ggaaactggg gctgaaaagc
     4321 accggacatg cgggcggtat tcacaactgg cggattgccg gacaagggct aagcttcgaa
     4381 caaatgctca aagagatggg caccgggctg gtggtgacgg agctgatggg ccagggcgtg
     4441 agtgccatta ccggtgatta ttcccgtggt gcagcgggct tctgggtaga gaatggcgaa
     4501 attcagtatc ccgttagtga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
     4561 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
     4621 ttgccggaga tgaaaatcgc cgggcagtaa aaaactggcg cgacctgccg cgctataaac
     4681 taaaaaatta cacaaaatca ttcgcactgc atcgagacgg aaagtgaatg agggccgggg
     4741 agcgtactcg cagtacgtga cccgggcgaa tgagcgtagc cgtcgatgag gcagtgtgaa
     4801 ggatgaagtg taaataaaaa aggaagtgag caatgcgtaa aagcctgtta gctattcttg
     4861 cagtctcctc gttggtattc agttctgcgt cgtttgccgc tgatcttgaa gacaatatgg
     4921 aaaccctcaa cgacaattta aaagtgatcg aaaaagcgga taacgcggcg caagtcaaag
     4981 acgcgttaac gaagatgcgc gccgcagcgc tggatgcgca aaaagcaacg ccgccgaagc
     5041 tcgaagataa atcaccggac agcccggaaa tgaaagattt ccgccacggt ttcgacattc
     5101 tggtcggtca gattgacgac gcgctgaagc tggcaaatga aggtaaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaga catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtaaag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgctgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 cgttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaaa ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgccac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgc atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tagagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg gggggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gccatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgactttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacattat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctctgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg ctcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc taggcgtgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac acctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggcaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 accgccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcagatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttatcggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagt ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcagggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaaacattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag catgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta aataactcca
    20701 ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag taaaatttgt
    20761 aatgcttaga cgttcttatt aactcaagga gttcgtcatg ggcaaaatat cagatttgaa
    20821 ttattctcaa cacattacat tagccgacaa ttttaaacaa aaaaatgaag ccttagatac
    20881 ctggtatgtg ggaatgaatg attttgcccg aattgccggg gggcagaata gcagaagcaa
    20941 tattctttct cctagagcat ttttggagtt tttggcaaag atatttaccc tgggttatgt
    21001 ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata ttgagttctc
    21061 atcttattct aaagatactg atggcaatga aaaaatgaag ttttacatga ataatcctga
    21121 aggggagcga gcggatttat ctaaggtgaa gatagaaatt acacttgcct cagcttctac
    21181 taaagggatt cgtgaaggac atacagtcat tatatttaaa caatctgatg gttcgactaa
    21241 tcgttatgaa gggaagtctt ttgaaagaaa agatgatagt tcattacacc tgattactaa
    21301 caaggttctg gcgtgttacc aacgcgaagc taacaagaaa atagcgcgtc tattaaataa
    21361 tcatcagaag ttaaataata tacaggagtt aaatgattcg caggagttaa ataattcaca
    21421 gaagttaaat aattcgcagg agttaaataa ctcgcaggtg agttgtaaag gttcagttga
    21481 ttctacgatt acggatttat tagaaaaagc attgaataa
//
LOCUS       DAJTUP010000065        21493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTUP010000065 DAJTUP010000000
VERSION     DAJTUP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.065"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO468_002542"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO468_002542"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR6259616.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO468_002543"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO468_002543"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR6259617.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO468_002544"
     CDS             complement(3121..3420)
                     /locus_tag="OO468_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR6259618.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO468_002545"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO468_002545"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR6259619.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO468_002546"
     CDS             complement(4632..5069)
                     /locus_tag="OO468_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR6259620.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO468_002547"
     CDS             complement(5115..5456)
                     /locus_tag="OO468_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259621.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO468_002548"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO468_002548"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR6259622.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO468_002549"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO468_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO468_002550"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO468_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6259623.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO468_002551"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO468_002551"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR6259624.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO468_002552"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO468_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR6259625.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO468_002553"
     CDS             12687..13217
                     /locus_tag="OO468_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR6259626.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO468_002554"
     CDS             13329..13865
                     /locus_tag="OO468_002554"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6259627.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO468_002555"
     CDS             complement(13973..14797)
                     /locus_tag="OO468_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR6259628.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="OO468_002556"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="OO468_002556"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR6259629.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO468_002557"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO468_002557"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR6259630.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO468_002558"
     CDS             complement(18729..19202)
                     /locus_tag="OO468_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6259631.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO468_002559"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO468_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR6259632.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21350
                     /gene="yiaK"
                     /locus_tag="OO468_002560"
     CDS             20352..21350
                     /gene="yiaK"
                     /locus_tag="OO468_002560"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR6259633.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21362..>21493
                     /locus_tag="OO468_002561"
     CDS             21362..>21493
                     /locus_tag="OO468_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6259634.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     "
BASE COUNT         5400 a         5488 c         5275 g         5330 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg ccaaaatcca ggcgttatga ggagataagt
    21361 catgattttt ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa
    21421 ggcgctcgat tttctgcgcg ctaccaactt caacgtcctg gagccgggcg ttgtcgaaat
    21481 cgacggcaag aat
//
LOCUS       DAJTUP010000066        20802 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTUP010000066 DAJTUP010000000
VERSION     DAJTUP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20802
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.066"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..340
                     /locus_tag="OO468_002562"
     CDS             <1..340
                     /locus_tag="OO468_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HCR6259635.1"
                     /translation="ESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVC
                     ISAIHLLDLFLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAG
                     GSSGGASGRW"
     gene            complement(400..1698)
                     /gene="eno"
                     /locus_tag="OO468_002563"
     CDS             complement(400..1698)
                     /gene="eno"
                     /locus_tag="OO468_002563"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR6259636.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1786..3423)
                     /gene="pyrG"
                     /locus_tag="OO468_002564"
     CDS             complement(1786..3423)
                     /gene="pyrG"
                     /locus_tag="OO468_002564"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR6259637.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3651..4442)
                     /gene="mazG"
                     /locus_tag="OO468_002565"
     CDS             complement(3651..4442)
                     /gene="mazG"
                     /locus_tag="OO468_002565"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR6259638.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4538..6772)
                     /locus_tag="OO468_002566"
     CDS             complement(4538..6772)
                     /locus_tag="OO468_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR6259639.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(6820..8121)
                     /gene="rlmD"
                     /locus_tag="OO468_002567"
     CDS             complement(6820..8121)
                     /gene="rlmD"
                     /locus_tag="OO468_002567"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR6259640.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8178..10934
                     /gene="barA"
                     /locus_tag="OO468_002568"
     CDS             8178..10934
                     /gene="barA"
                     /locus_tag="OO468_002568"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR6259641.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11166..12506)
                     /gene="gudD"
                     /locus_tag="OO468_002569"
     CDS             complement(11166..12506)
                     /gene="gudD"
                     /locus_tag="OO468_002569"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6259642.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12527..13867)
                     /locus_tag="OO468_002570"
     CDS             complement(12527..13867)
                     /locus_tag="OO468_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR6259643.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(13869..15221)
                     /gene="gudP"
                     /locus_tag="OO468_002571"
     CDS             complement(13869..15221)
                     /gene="gudP"
                     /locus_tag="OO468_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR6259644.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15655..16104)
                     /locus_tag="OO468_002572"
     CDS             complement(15655..16104)
                     /locus_tag="OO468_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR6259645.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16122..16904)
                     /gene="truC"
                     /locus_tag="OO468_002573"
     CDS             complement(16122..16904)
                     /gene="truC"
                     /locus_tag="OO468_002573"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR6259646.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(16904..17233)
                     /locus_tag="OO468_002574"
     CDS             complement(16904..17233)
                     /locus_tag="OO468_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR6259647.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(17855..18400)
                     /gene="syd"
                     /locus_tag="OO468_002575"
     CDS             complement(17855..18400)
                     /gene="syd"
                     /locus_tag="OO468_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR6259648.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18468..19316
                     /gene="queF"
                     /locus_tag="OO468_002576"
     CDS             18468..19316
                     /gene="queF"
                     /locus_tag="OO468_002576"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR6259649.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19428..20792
                     /gene="ppnN"
                     /locus_tag="OO468_002577"
                     /gene_synonym="ygdH"
     CDS             19428..20792
                     /gene="ppnN"
                     /locus_tag="OO468_002577"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR6259650.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5075 a         5575 c         4980 g         5172 t
ORIGIN      
        1 agaaagtgaa tcaacgtctt ccactgatca ttattacttt gctatttttt gggtatttgc
       61 agtgatgttc ttccctttct ggttttttca tcaaggcagt aatttttgtc gcgcatgtaa
      121 aagtggagtc tgtatttcag caatccatct tttagattta ttcctgttct ccgataaaac
      181 tttttccatt gctgtatttt ccttcttttt tactttcacc atatttatgg tctttacctg
      241 tttatgtgta cttcagaaaa gagcatctgg tagaagttat cattcagaca acagcggttc
      301 cgctggaggc tcaagtggcg gcgcatccgg ccgctggtaa ctccaccata aaaaatgcca
      361 gcccgaaggc tggcattatc aaatcagata aagtcagact tatgcctggc ctttgatctc
      421 tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa tcagctggtt
      481 gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc ctgcagcagt
      541 accaacagcc agatcagcga tggtagcatc ttcagtttca ccagaacggt gagagataac
      601 tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg tcagagaacc
      661 gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac cttctttcag
      721 gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt cgcccagaac
      781 tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt cgatagaaac
      841 gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag aggtgaacgc
      901 tttgttgcct tcgccagcca gaacgtattt accatctttg tagaattcag aagctgcgca
      961 gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa cagcttcagc
     1021 gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac cttcgtcacc
     1081 aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga aaacttcaga
     1141 acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca tgaattcctg
     1201 gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca tcggaaccgg
     1261 catggagtat ttgcccggag tgccgttcag ttcagcgatg tgctcgtaca gcggcatacc
     1321 tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg cgttcgcgcc
     1381 gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg
     1441 atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc
     1501 tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc
     1561 acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac caccctccag
     1621 atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac gaccgatgat
     1681 ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa actccagaca
     1741 aacaacgcgt acccaaggta cgcgttgccg ttctaacttt tttacttact tcgcctgacg
     1801 tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac agcgggtgac catcacgtgg
     1861 agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat tcggaacttc
     1921 gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac ctgcatcttc
     1981 aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct caacaattgt
     2041 cggcgcattg tatagctggc gaaccaggct atcgtcaacc aactggcact gctgtgcgcc
     2101 gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt tgccgttttc
     2161 atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca caaattctgt
     2221 agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat
     2281 acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat
     2341 catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcgt ccagaccttt
     2401 aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga tgttgacgct
     2461 gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat aagcatccgg
     2521 cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccggat tcgcttcttc
     2581 gaagataacc tgttcccatt cggacagatt tgcttccggg cagtttaagc tgaatcgttt
     2641 acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat agatggaatc
     2701 gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg caatcttcgc
     2761 acgttcgttc gccggtacag cgcgatctga acgacaaatc aggatgtcag gctggatacc
     2821 gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt caccagacgc
     2881 tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc caatttcaac
     2941 agccatctgg cgaatagctt cgaggaacgg caaggattcg atatcaccta ctgtaccgcc
     3001 gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct ctttgattgc
     3061 gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc ggcgttcttt
     3121 acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc tcattttggt
     3181 acgaatgaaa cgctcgtagt gccccaggtc caagtcggtt tcagcgccgt cttcagtaac
     3241 gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga tgtaaggatc
     3301 cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga gggaggctgc
     3361 ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat agttcgttgt
     3421 catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaagac gggaaagcag
     3481 tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg ccagcgcact
     3541 attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac gcaaatcaag
     3601 cgcttgtcat ttaaaaaatg acacaaatgg cgcttgaccg cgtaattccc ttagagatca
     3661 atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc aacacctgtc
     3721 atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg ctcgaatttt
     3781 tcgttcactt tttgcaatgc gatttctgct ttcgtcccta aatggcgagc cagattaacc
     3841 gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac aacagcctgc
     3901 cgcgcctcgt acatcacctc gtcgatctct tcgtagactt tatcaaccac cgggccaagc
     3961 gtcgtccagt cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc ccgcattaaa
     4021 gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg cgcgcgctct
     4081 tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc agaactatca
     4141 gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca aatatcatta
     4201 aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg gaatagcaga
     4261 tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg
     4321 taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt atcccacggg
     4381 cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacggtc gatttgattc
     4441 attgaattgt cctgaaaatt gcgggtctgt caggtgtaat accttcgagt aaaagatatc
     4501 gctggatacg gctgacgcca tatccagcta tgtaagacta actcccgtgc aaccgacgcg
     4561 cgtcgataac atccggcacc tggttgagtt tacccagcac gcgccccagc acttgcaggt
     4621 tgtaaatctc aatggtcatg tcgatggtcg ccagttgctg tttggtgtcg ctacggctgg
     4681 caacgccaag cacgttcacc ttctcgttgg cgagaatggt cgtgatatca cgtaacaacc
     4741 cactacgatc attagccacc acgcggacca ccagcgaata tccggcggag tagctctcac
     4801 cccataccgc gtcaacaatg cgttctggcg catgggagcg cagttccgcc agttgttcgc
     4861 aatcggcgcg gtgtactgaa ataccgcgtc cctgggtaat gaagccgaca atctcatctc
     4921 caggaatcgg ctggcagcag cgcgcgatgt ggtgcatcag gttaccaaca ccttcaacca
     4981 ccacacgacc gttatcttta ctgcggtttt gcggtgtgta gcttttttgc tgaagctgtt
     5041 tcagcgcggc ggcgtcctgc tcttcggcac tcggcttatt aaattgcgat tgcaggaagt
     5101 tcaccatctg attgagacgg atatccccgc caccaatcgc cgccagcaac tcgtcgacat
     5161 cattgaagtt gtaacgcggc agcagatgtt tttctgcttc tttcaggctg atccccagat
     5221 gttccagctc gtcgtcaagg atttgccgcc cagccagaat gtttttgtca cggtcctgtt
     5281 tacggaacca ggcgtgaatt ttcgaacgcc cacggctggt tgtgacgtaa ccgaggtttg
     5341 ggtttaacca gtcacggctg gggttcggct gtttctgggt gataatttca atctggtcgc
     5401 ccatttgcag ctggtaggtg aacggcacaa tgcgcccgcc aatttttgcc ccgatgcagc
     5461 ggtgtccgac atcactgtgg atgtggtaag cgaagtccag cggcgttgat cccgcaggca
     5521 aatcaacgac atcacctttc ggcgtaaaga catacacccg gtcgtcaaac acctgactac
     5581 gtacttcgtc gagcatttcg ccggaatcag ccatctcttc ctgccacgca atcagtttac
     5641 gcagccaggc aatccggtct tcatgtcccg aacgtgcgcc gccagctgcc gcgccctctt
     5701 tatatttcca gtgcgcagca acacccaact ccgcatcttc atgcatctgt ttggtgcgga
     5761 tttggatctc aacggttttt ccacccggcc ccagaaccac ggtatgaata gactgataac
     5821 cgtttggttt cgggttagcg acgtaatcgt caaactcatc cggcaggtgg cgatagtgag
     5881 tgtgcactat ccccagtgcg gcataacaat cctgtaaacg ctcggcgaca atacgtaccg
     5941 cacgcacatc aaacagctca tcgaaggcga ggttcttttt ctgcattttg cgccagatgc
     6001 tgtagatgtg tttcggacga ccatacactt cagctttaac gccttcagct ttcatctcag
     6061 cgcgcagatg gccgacaaac tcttcgatat agtgttcgcg gtcgagacgg cgttcatgca
     6121 gcagttttgc gatgcgtttg tattcggtcg ggtggagata acggaagcag taatcttcca
     6181 gctcccattt cagttgccca atcccaagac ggttcgccaa cggcgcgtag atattggtgc
     6241 actcttttgc cgccagtaca cgttcatctt ccggcgcatc ttttacttcg cgcagatgag
     6301 caatacgctc cgccagtttg atgactacgc agcgaaaatc atcgaccatc gccaataaca
     6361 tccggcgaac gttatcgacc tgttcggagg aaacagaatc agtgtgcgtc gctttcagct
     6421 ggcggatcgc cgccatatca cgcacgccgt gaataaggtt aacaaccgac ttaccgacgc
     6481 tctcacgcag cacatcttcg ctgactacgt tggcatcagc cagagggaac agcagcgccg
     6541 cccgcagcgt gtcaatgtcc atacttaatg tcgagaggat ctccaccatc tcaacaccac
     6601 gccacaataa cagactggca tccggatgcc cctgcgtctg ttgcagacaa tacgcccagg
     6661 tttcggctaa gcactcacac gacttctggc tggtaatacc cagacttgcg atccattttt
     6721 ccggatcaaa ttcaccagcc ttattgatat gtgcacttct taccgcaacc atcgtcctct
     6781 cctttaggga ccagacctgc cgaaatcggc aaatcgtaac tatttaacgc gcgagaaaag
     6841 taccatcgat tccagatgtc ccgtgtgtgg gaacatatcc agcatcgcca gtcgcgcaat
     6901 ggtatatcct gcttttaata acgcttcgct atcccgagcc agcgttgcag ggttacagga
     6961 tacataaact atacgaatag gttccagttt tataatttgc tgcataacac ctgcggcacc
     7021 tgctcgcgcc gggtccagca acactttatc gaagccgttt ttcgcccacg gctgctttgt
     7081 gacatcttct tcaagatttt cgtgataaaa cgtcacattc tgtaagccat taagacgcgc
     7141 attctgctgg cctttttcca ccagcgcagg aacaccttct acaccgacca cactggcagc
     7201 ttgtgtcgcc aatggcagtg taaagttgcc cataccgcag aacagatcca gtacgcgatc
     7261 ttcaggttgc acatccagcc attccaacgc acgcgctacc attttttggt tcacacccgc
     7321 attgacctga ataaaatcgc gcgggctaaa agttaagcgc aacccgtttg agtcatacca
     7381 gggcatctca ccagagacgg tttcgagtat ctcactatcg ggggcgagat acagatccag
     7441 gccttcagaa tgcgaaaagc gttccagttt ttcgcgatct gccgaactta gcggtgcggt
     7501 atggcgcaaa atcatcagcg tgccgctggt tgcctgtacc agttcaacat gaccaagatg
     7561 gcgcatagct tgtaagctgc ccagacatgc cctgactttg ggcagcaatg cttcaagttg
     7621 gggcgctaaa atggggcatt gtttgacgtc gacaatgtca ctggagcccg ctttgcgaaa
     7681 ccccatctga agttgctgtg ttttcggtaa gtagttcaga cttaaacgcg cgcggcggcg
     7741 atagccccag ggaacatcgg cgatcacttc agagacatcg tgtttcatta atcgggcgag
     7801 tgccgcactt ttgcttcgct gctgtaaatc cacgctggcg tgttgttgct gacagccgcc
     7861 gcatacgcca aaatgaggac agcgtggcgt ttcgcgttcc gggctatcgc ttaaccggcg
     7921 tacgacttta gcgcgggcat actgtttttt atcttcagta acagcaactt ccgcgttttc
     7981 ctgcggcaat aatccgggga taaataacgt tttgccgtta tgtcgcgcca cgccctgacc
     8041 aaaagagtcg aggtcgttga ctgaaacggt tatgatctga cgcgtcgtcg tgcgtcgttt
     8101 tgcagagtag aattgcgcca ttggcgagac tttctcaatt taacagtgtg accttaattg
     8161 tcccataacg gaactccatg accaactaca gcctgcgcgc acgcatgatg attctgatcc
     8221 tggcaccgac cgtccttatt ggtttattgc tgagtatctt tttcgtcgtg catcgctata
     8281 acgacttgca gcgtcaactg gaagatgccg gtgctagcat tattgagccg cttgcagttt
     8341 ctactgaata tggcatgagc ctgcaaaatc gcgaatctat cggtcagtta ataagcgtac
     8401 tgcatcgtcg ccattccgat attgttcgcg cgatttcggt ttatgatgaa aataaccgac
     8461 tctttgtcac ctccaatttt catcttgatc cctcatcaat gcagctcggc agcaacgtgc
     8521 cgtttcctcg ccagctcact gtcactcgtg acggcgatat tatgatcctc cgcacgccga
     8581 ttatttctga gagttactcc cccgacgaat cgcccagtag cgatgccaaa aatagtcaaa
     8641 atatgttggg atatattgcg cttgagctgg atcttaaatc ggttcgcttg cagcaatata
     8701 aagagatctt tatttcctgc gtgatgatgc tgttttgtat cggtattgcg cttatttttg
     8761 gctggcgctt aatgcgcgat gtaaccggtc cgattcgcaa catggtgaat accgtcgacc
     8821 gcatccgtcg cgggcaactc gacagccggg tggaaggatt tatgctcggc gagctggata
     8881 tgctgaaaaa cggtatcaac tcgatggcaa tgtcgctggc tgcttatcac gaagagatgc
     8941 agcacaatat cgaccaggcg acgtccgatc tgcgtgaaac gctggagcag atggaaattc
     9001 agaacgttga gttggatctg gcgaaaaagc gcgcccagga agcggcgcgt attaaatccg
     9061 agtttctggc aaatatgtca cacgagctgc gtacaccact gaatggtgtt attggcttta
     9121 cccgcctgac gctgaaaaca gaattaacac caacgcagcg cgatcacctg aatacgattg
     9181 aacgttcggc aaataatttg ctggcaatta ttaatgatgt tctcgacttc tcgaaactgg
     9241 aagcaggtaa gctgattctg gaaagtattc cattcccact acgcagcacg ctggatgaag
     9301 tcgttactct gctggcacat tcttctcacg ataaagggtt ggaactgacg ctcaatatta
     9361 aaagcgacgt gcctgataac gtgatcggcg acccactgcg attacagcaa atcatcacta
     9421 acctggtggg gaatgcaatt aaattcaccg agaatggcaa cattgatatt ctggtagaaa
     9481 aacgtgcgct gagtaatacc aaagtgcaga ttgaagtgca gattcgggat accggcattg
     9541 gtattcctga acgcgatcaa tcgcgcttat tccaggcctt ccgacaggct gatgccagta
     9601 tttcccgccg tcatggtggc accgggctgg ggctggtgat tacacaaaaa ctggttaatg
     9661 aaatgggcgg cgatatttcg ttccatagcc agccgaatcg cggttcaact ttctggttcc
     9721 acattaatct cgatctgaac ccgaacatta ttatcgaagg gccatccatc cagtgcctcg
     9781 caggtaagcg cctggcctat gtcgaaccaa actccgcagc agcgcaatgc acgctggata
     9841 ttttaagtga aacgccgctg gaagtggttt atagcccaac gttctccgcg ctgcctcccg
     9901 cgcattacga catgatgttg ttaggcatcg cggtgacctt ccgcgagccg ctaacaatgc
     9961 aacatgagcg attagcgaaa gcggtatcga tgaccgattt cctgatgctg gcacttcctt
    10021 gccatgcaca agttaatgct gaaaaactca agcaagatgg tatcggcgcg tgtctgctga
    10081 aaccattaac acctacgcgc ctgttgcctg ccctgacgga attttgtcat cacaaacaaa
    10141 acacgctttt gcctgtaacc gatgaaagta agctggcaat gacagtcatg gcggttgatg
    10201 acaaccccgc taacctgaaa cttatcggcg cattgctgga agatatggtg caacatgtgg
    10261 aactttgcga tagcgggcat caggcagttg aacgggcgaa acagatgccg ttcgatttga
    10321 tcttaatgga tattcaaatg ccggacatgg atggcattcg ggcctgcgaa ctcatccacc
    10381 aactcccgca tcagcgacaa acgccggtta tcgcggtaac ggcgcatgca atggccgggc
    10441 aaaaagagaa gctgcttggc gcagggatga gcgattatct ggcgaaaccg attgaagaag
    10501 agcgattgca taatttgttg ttgcgctaca agcctggcag cggtatttcg tctcgcgtcg
    10561 tgacgcccga agtcaacgaa attgtggtga acccgaatgc gaccctcgac tggcaactgg
    10621 cactacgcca ggcagcagga aaaaccgatt tagcgcgcga tatgctgcaa atgttactcg
    10681 atttcctgcc tgaagttcgc aacaaagttg aggaacagct ggctggagaa aacccggaag
    10741 gcctggttga tttgattcat aaactgcatg gcagttgcgg ctatagcggc gtaccacgta
    10801 tgaagaatct ctgccagctg atcgaacaac agctacgtag cggtactaaa gaagaagatt
    10861 tggagccgga gctgctggaa ctgttggacg agatggataa tgtcgcgcgc gaagccagca
    10921 aaattctcgg gtaacggacg tcttgtcgag tagcaatcgg ctttttatga cgctggtttc
    10981 agattggtga caaagtgcct gtgtttatgc cggatgcggc gttaacgcct tatccggcct
    11041 acaaaaccaa ttaaattcaa tgaattaaaa aatgatgtag acctgataca tagcacaaca
    11101 ggctattttg cgtttagcat cagtctcaaa ccggctccag atagagccgg ttttggtttt
    11161 ctgtcttaac gcaccatgca cgggcgcttg ttatcgaacg tccagccagg aatcagatac
    11221 tgcattccca tcgcatcgtc acgcgcgcca agcccgtgtt tctgatacag ctcatgggct
    11281 ttcatcactt gatccatatc gatttctaca cccagccccg gtttttctgg cacctgtacc
    11341 agcccgcctt tgatctcaaa cggttctttg gtcaggcgct gattaccttc ctgccagatc
    11401 cagtgcgtat caatagcagt aattttaccc ggtgcagcgg cggcaacatg ggtaaacatc
    11461 gccagggaaa tatcgaagtg gttgtttgag tgtgaacccc aggtcaggcc aaattcatgg
    11521 cacatttgcg ccacacgtac cgaaccttgc atagtccaga aatgcggatc cgccagcggg
    11581 atatcaacgg attgtaacga gagcgtatgg cccatctgcc gccagtcggt ggcgatcata
    11641 ttggttgcag tcggcaggcc agtcgcgcga cggaactctg ccatcacttc acgcccggag
    11701 aaaccttgct ccgcaccaca cggatcttct gcataagcca gcgaaccttt caggtattta
    11761 ccaattttaa ttgcttcgtt cagcgaccag gcaccgttag gatcgagcgt aatacgcgcc
    11821 tgcgggaagc gttgcgccag tgccacaata gactcggctt cttcttcccc ggccagtaca
    11881 ccgcctttca gtttgaaatc gttgaagcca tatttttcat acgccgcttc cgccaggcgc
    11941 accaccgcat ccggcgtcat cgcttcttca tgacgcaggc gataccagtc gcatgagtca
    12001 tccggctggc tttgatacgg cagaggtgtg gctttgcgat taccgacgaa gaacagataa
    12061 ccgagcattt cgacttcgct acgctgttga ccatcgccca gcagcgatgc cacgtttacc
    12121 cccagatgct gccccagcag atccagcatt gccgcttcta tcccggtaac tacatgaata
    12181 gtggtacgca aatcaaatgt ctgcaaaccg cgcccactag catcacgatc ggcaaaagta
    12241 ttacgcacca gcgtcagaac gtttttgtat tcacccagcg ttttacctac caccagcgga
    12301 atcgcatctt ccagcgtttt acggattttc tcgccgccgg gaatttcccc tacgccagtg
    12361 tgaccggaat tatctttgat aatcacaata ttacgcgtga agaacggtgc gtgtgcacca
    12421 ctcagattca tcagcatact gtcatgaccc gccaccggga tgacctgcat ttcagtaaca
    12481 acaggcgtcg taaattgaga actcatattt atgtcctttt tcagaatcaa tgacggccga
    12541 aaacgggacg tttacggtca aaggtccagc cggggatcag gtactgcatc ggacctgcgt
    12601 cgttacgcgc accgcaaggc agacgtttat aggcctcatg tgccttttgt acctgttccc
    12661 agtccagttc cacgcccaga ccgggcgcat caggaacggc aatttttccg tttttaatct
    12721 ccagcggatt tttggtcagg cgacaatcgc cctcctgcca aatccagtgg gtatcgatag
    12781 cggtaggatt acccggtgcc gccgcgccca catgggtaaa tatcgccaga gagatatcga
    12841 aatggttgtt ggaatggcag ccccaggtca gcccccagtc gtcgcaaagc tgcgccacgc
    12901 ggactgcacc ggaaagcgtc cagaagtgcg gatcggcaag tggaatatct accgcattga
    12961 gcatcaccgc atgacccatt tcgcgccagt tggtggcgat catgttagtc gcgacgggca
    13021 agccggtcgc ccgtcgaaac tccgccatca cttcacgtcc ggagaagccc tgttctgcgc
    13081 cgcatggatc ttcggcatag gtaagaacat cattcagccc tttgcataaa gaaatggctt
    13141 catcaagcag ccatgcaccg ttgggatcaa cggtaatccg cgcatccggg aagcttttct
    13201 tcaatgcacg aacagtgtcg atttcttgct cgccaggtaa caccccgccc ttaagtttga
    13261 aatctttaaa gccatagcga tcctgtgagg cttccgccag acgcacaacg gcttcgctgt
    13321 tcatcgcttt ctgatggcgc aactgatacc actcatggtt gcccggcgta ttttccagat
    13381 aaggaagatc ggttttggtc cgatcaccta tataaaacag ataaccgagg acggtaatag
    13441 tctcgcgttg cttgcctggc cccaacagtt cgcagaccgg aacattcagc gccttaccta
    13501 gcaggtcaag caaggcggct tccagcgccg ccacggcatt aacgcgcaat tcaaaagtcc
    13561 aggcaccttt gccaaaggta tcaaaatcgg ctgcctgatt acctttatgt acctgctgaa
    13621 ccactttatt caggcgcgca acttcctggc ccagaaccat cggaatagca tcgaccagtg
    13681 tttgataaat cacctctccg cccggcgctt caccaatgcc ggtatgcccg gcgttatcgg
    13741 tgagtaccac gatattgcgg gtgaagtatg cgttatgcgc accaccaata ttgagcaaca
    13801 tgctgtcatg tccggcgacc ggaatgactt tcatatcagt aataacagga ctggattgtg
    13861 tcgccatctt attgccccgc gacaggtttc agctcgatac gcttgatatc gcccaccagc
    13921 accaggtagc tcagtaccgc gattagggca tgaacaccaa cataaatcag cgccccatta
    13981 aacgagccag tcgtgccaac gatataacca attgcgattg gcgtgacgat gccagaaatg
    14041 ttaccgaaca tgttgaacag gccaccggaa agaccgctga tctcttttgg cgcggtatct
    14101 gccattactg cccaacccag cgccccgatg cccttaccga agaaggccag cgccataaag
    14161 ccgatgatca tccactcaac gttgacgtag ttgcagaaca ccatcaccat cgacagcaac
    14221 atgcccatta cgatcggtgt tttacgcgca atgttcagcg atcccgtgcg gcgcatcagc
    14281 cagtcggaaa taattccacc cagcacaccg ccgataaaac cgcaaaccgc cggaacggaa
    14341 gccacaaagc ccgctttcag aatcgacatc ccacgcgcct gcaccagata aaccggaaac
    14401 caggtaatga aaaagtaagt cagggcgttg atacagtact gaccgatata aacgccgatc
    14461 atcatccgtg acccgagcag ctgtttgatc tgcccccact tcacgctgaa cggcacttta
    14521 actttggtgc tttgctgatc catattgatc agcgcgccac ccgcggcgat atactccagc
    14581 tctttctgat ttacccccgg atgttgattt ggctcgtgga tgactttcaa ccagataaag
    14641 ctgatgacaa tgcccagacc gcccataaag aagaagacgt gtgaccagcc cacttcatgc
    14701 gtcagccagc ccataatcgg cgcgaagatc accgttgcga agtattgagc ggagttaaaa
    14761 atcgacaccg ccgttcccct ttcctgcacc ggaaaccagg ccgcaacaat gcgactgttg
    14821 ccggggaaag atggcgcttc agcaagcccg accaggaagc gcagagtaaa cagggcaacg
    14881 ataatgccga atccactaaa gatatcgacg aagccttgca gcaaggtaaa catcgaccag
    14941 ataaagatcg accagaagta gacgcgtttt gaaccaaaac gatccagcaa ccaaccacca
    15001 gggatctgcc cgataacata agcccatgag aaggcagaga acacatagcc cattcccacg
    15061 ggatcaaggc cgatatcttt ggccatttcc gaaccggcaa tagaaagcgt agcgcggtcg
    15121 ccgtagttga aggatgtgac gataaacaac atcaccacta tccagtaacg agcattcgtg
    15181 cgtttttcca cactgctcgc agcctgactt aaagaactca ttgttgcact cctgaaaatt
    15241 cgcgttagcc acgctcactc tggactgcga catcgccagg aaatcagagg tgacgtaggg
    15301 tgttttttgc cgtttttata ggtcgttcgc cgaatacggc gcgtgtttat atctggcaat
    15361 agcagtataa aaagcgcgcc atagcggctc accgtgcaac aacacaacat taatgcgttc
    15421 aatgaggccc gattttggca ttagccctgg acggtggaat ccacttcacg gaaatgaaaa
    15481 caagaacaag aaaggaaggg taaaaacgaa gaaataaaga agagtatgaa atggatcgct
    15541 tgactctagg caaacgccag taaaaatccg cgctatgaag cagtttttac tggcatttgc
    15601 ctgaaaagat tcgattcagc accgctaaaa cgacatttac cgctcgctga catatcagga
    15661 caacagcgtg ccccactgtt cgacccacgg atttgattcc gtttccggtt ccgggttctc
    15721 actggcgtca atcaacagca tttcgccaac ccgctgagcg ctctgttcct gcaacaaggc
    15781 atcgaactgt ttgccgccat tgcaaaaatt tacataactg ctgtcgccga gcgcaatcac
    15841 gccataacgc agattcggct ggaagcccag actatctttg attccctgaa agagcggcac
    15901 aatgctatca ggaaggtcgc cctgcccggt cgtggacgta accaccagaa catacttatc
    15961 ctgatagggc agccagtcgc ttaattcagg atcttcaaat accgttgctt tgtggccctg
    16021 tgcggtcaga attgcttctg cttcttccgc cactaacagc gaatttccgt acatggtgcc
    16081 gacaaaaata ccaatttccg ccataccgtt ttctccctgg attaggaact tatctctcca
    16141 tcctgacccg atggcgcact gaactcaacc ctttcatttt caggaagcag accgcgccag
    16201 ccaaattgtg ataacgcctg catccaggtg tcgtccaggc ccgcgtgaat agtcagcggc
    16261 tcgccagtaa aaggatgcgt caatgacaac tgactggcat gtagcattaa ccgctggagg
    16321 ccaaaatgct cagcaccgct gcgattctgg cgtaaatcgc catgtttgct atcgccaata
    16381 atcggatgac gcaaatgggc aagatgtcgt cgaagctgat gtttgcgtcc ggttttcggc
    16441 tccagttcca ccaggccgta gcgcgtggtc gggtaacgtc cggtcgctac cggcatttct
    16501 acggtcgcca gaccgcgata atgcgtcact gctggctgcg ggcctttatc ttcgcgggca
    16561 aatttatcag cgattttgtc cagttcttcc accagtggat aatccaacac cgcttcttcc
    16621 atcaaccagc cgcgcacaat cgcatggtaa cgtttctgga tttggtgctg ttcaaactgt
    16681 tgtgccagca gccgtccggc ctcgctggat aatcccatca acaacacacc agaagtgggt
    16741 cggtccagac gatgagcagt aaaaacatgc tgtcctatct ggtcacgcac ggtttgcatg
    16801 accactactt tctcgtcgcg atccagccag ctacggtgaa ccagccagcc ggagggttta
    16861 tttaccgcaa ccagccattc atcctgatag agtatttcca gcattagctc gcatcatccg
    16921 caaaaagagc atccagtttt tccaactcag ccagaataag cgcgcgttgc ggatggtccg
    16981 tcgccagcgc catttcataa tagggtgcaa cagcaaaagc gcccggtaac ggctggttgt
    17041 tatttaacaa atcgtgcatt cgcgggatca gcccccactg caaccactcc agtggttcca
    17101 tggtgtccat aaagaacggt tgggtactat taaattgatg cggctggggt tcatcgtttc
    17161 gccagtgctg atgttcacgc agtaacgctt caagcgcctg caactggaga cgaacgcggt
    17221 catgagtggt cataaagcaa cctcaataag aaaaactgcc gcgaaggata gcaggaataa
    17281 aaaaagggag cactgtattc acagtgctcc cggttcgttt cgcagcattc cagctacttt
    17341 tgttgctccc tgctcatcct tgacaacttt tcctctggcc ttgcggccaa tcgttcatcc
    17401 tgaactattg cttcctgctc acaccacccc gatgtgatac ttcatcctga agtgtccctg
    17461 gccttcctga cccaccgaat catcctgacc ggttctcatt ctccatcctg gaggtgtcct
    17521 ttaacgcgtc ctgcgtcatc ctcttcgctt catccagaag cctttccctg aaacaccatc
    17581 ctggtgtgtc ctgcagaagt gtcatcatcc tgatgttcac ttcgttgtct gactccctgt
    17641 cgacgaagat agaatcgtct ttttcgcgaa gtcttacaag gcgcttacag acaatgcctt
    17701 aaggaaaatt tcatatgaaa gttaagaatt aacaatcaac attttgattt taaatgaaaa
    17761 taacgattct tatcttttta agtattcgct tatttcctgg cgatctccta cagagtctgt
    17821 aagagatctc tcacaaccag atggggtatt tggattacag aagaggctta agttgattaa
    17881 ggaattccgc aagatttgag gctaaattcg tccgtttacg tgttcccagc gtctctttgc
    17941 acacttcacc ggagagatta cacaccgaga tgacctctaa ctcttcttcc agcgtcgcga
    18001 taaatagcgt gggcggcagt tttagccttt tctgagtcac cagatgacca atcagatttt
    18061 cctgcacccg acggaagtcg tcttcactcc aggtttgcag caatgtcagt ttgatatcgc
    18121 caaactgcgc gtgcatatcc ccggcaaact gagtcgtata aaaggtatga attgtgggtt
    18181 gtatcacaat atcaaaagcg cgttcaaccg cgtttacatt ttgttccccg gtaaacggct
    18241 ggggttgcca gtatacggca tcttcggtag ttgaaataat gcacggtgaa ggaacaccgt
    18301 acaattcctc acttaacggc cagcttttat gctcttcatg ccacgcatcg cagtagcgtg
    18361 cagtaaaatc tttcagggct tgtgcggtca aatcgtccac taatttctct cttcacttaa
    18421 accagataca cttgtcgttt agtttatctg gtttatgacg gtgaaacatg tcttcttatg
    18481 caaaccatca ggcacttgcg ggcctgactc ttggaaaatc aaccgattac cgggatacct
    18541 atgacgccag cctactgcaa ggcgttccac gcagcctgaa tcgcgacccg ctgggtctga
    18601 aagcggataa cctgcctttt cacggtacgg atatctggac gctgtatgaa ctttcctggc
    18661 tgaatgcgaa aggtttgccg caggtcgctg tcggtcatgt tgaacttgat tacaccagcg
    18721 taaatctgat tgagtcgaag agttttaagc tctatctcaa cagttttaac cagacgcgtt
    18781 ttaataactg ggatgaggtg cgccagacgc tggagcgcga cttaagcact tgcgctcagg
    18841 gtaagattag cgtggcgtta tatcgtcttg atgaactgga aggccagccg ataggtcatt
    18901 tcaatggcac ttgcattgat gaccaggata tcactatcga taactatgaa ttcactactg
    18961 actatctgga gaatgccacc agcggtgaaa aagtagtgga agagacgctc gtcagccacc
    19021 tgctgaaatc aaactgcctg atcacccatc aaccagattg gggttcgatc caaattcagt
    19081 atcgtggacg ccaaattgac agagaaaaac tgctgcgtta cctggtctca ttccgtcatc
    19141 acaacgagtt ccacgaacag tgcgtggaac gcatctttaa tgacctgtta cgcttctgcc
    19201 agccagagaa attgagcgtt tacgcacgtt atacccgtcg tggcggtctg gacattaacc
    19261 cgtggcgcag taatagcgat tttgtcccat cgaccacaag actggttcgg caataaattt
    19321 tttctcaatt ttgcgtgctg gattcacgca gaaggttgtg aaaggtcatc aggcagggct
    19381 attgtaatca aagggaatga cgatattcgt cccataagga gtttttcttg attacacata
    19441 ttagcccgct tggctccatg gatatgttgt cgcagctgga agtggatatg cttaaacgca
    19501 ccgccagcag cgacctctat caactgtttc gcaactgttc acttgccgta ctgaactccg
    19561 gtagtttgac cgataacagc aaagaattgc tgtctcgttt tgaaaatttc gatattaacg
    19621 tcctgcgccg tgaacgcggc gtaaagctgg aactgattaa tcccccggaa gaggcttttg
    19681 tcgatgggcg aattattcgc gctttgcagg ccaacttgtt cgcggttctg cgagacattc
    19741 tcttcgttta cgggcaaatc catcataccg ttcgttttcc caacctgaat ctcgacaact
    19801 ccgtccacat cactaacctg gtcttttcca tcttgcgtaa cgctcgcgcg ctgcatgtgg
    19861 gtgaagcgcc aaatatggtg gtctgctggg gcggtcactc aattaacgaa aacgagtatt
    19921 tgtatgcccg tcgcgtcggg aaccagctgg gcctgcgtga gctgaatatc tgcaccggct
    19981 gtggtccagg agcgatggaa gcgccgatga aaggtgctgc ggtcggacac gcgcagcagc
    20041 gttacaaaga cagtcgtttt attggtatga cagagccgtc gattatcgcc gctgaaccgc
    20101 ctaacccact ggtcaacgaa ttgatcatca tgccggatat cgaaaaacgt ctggaagcgt
    20161 ttgtccgtat cgctcacggc atcattatct tccctggcgg tgtgggtacg gcagaagagt
    20221 tgctgtattt gctgggaatt ttaatgaacc cggccaacaa agatcaggtt ttaccattga
    20281 tcctcaccgg cccgaaagag agcgccgact acttccgcgt actggacgag tttgtcgtac
    20341 atacgctggg cgaaaacgcg cgccgccatt accgcatcat cattgatgac gccgctgaag
    20401 tcgctcgtca gatgaaaaaa tcgatgccgc tggtgaaaga aaatcgccgt gatacaggcg
    20461 atgcctacag ctttaactgg tcaatgcgca ttgcgccaga tttgcaaatg ccgtttgagc
    20521 cgtctcacga gaatatggct aatctgaagc tttacccgga tcaacctgtt gaagtgctgg
    20581 ctgccgacct gcgccgtgcg ttctccggta ttgtggcggg taacgtaaaa gaagtcggta
    20641 ttcgcgccat tgaagagttt ggtccttaca aaatcaacgg cgataaagag attatgcgtc
    20701 gtatggacga cctgctacag ggttttgttg cccagcatcg tatgaagttg ccaggctcag
    20761 cctacatccc ttgctacgaa atctgcacgt aatctcccct cc
//
LOCUS       DAJTUP010000067        20658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTUP010000067 DAJTUP010000000
VERSION     DAJTUP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.067"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..144)
                     /locus_tag="OO468_002578"
     CDS             complement(<1..144)
                     /locus_tag="OO468_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HCR6259651.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSV"
     gene            complement(199..1050)
                     /gene="manZ"
                     /locus_tag="OO468_002579"
     CDS             complement(199..1050)
                     /gene="manZ"
                     /locus_tag="OO468_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR6259652.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1063..1863)
                     /gene="manY"
                     /locus_tag="OO468_002580"
     CDS             complement(1063..1863)
                     /gene="manY"
                     /locus_tag="OO468_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR6259653.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(1926..2897)
                     /gene="manX"
                     /locus_tag="OO468_002581"
     CDS             complement(1926..2897)
                     /gene="manX"
                     /locus_tag="OO468_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR6259654.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3360..4910
                     /gene="yoaE"
                     /locus_tag="OO468_002582"
     CDS             3360..4910
                     /gene="yoaE"
                     /locus_tag="OO468_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR6259655.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(4914..6512)
                     /locus_tag="OO468_002583"
     CDS             complement(4914..6512)
                     /locus_tag="OO468_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6259656.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6643..8007)
                     /gene="sdaA"
                     /locus_tag="OO468_002584"
     CDS             complement(6643..8007)
                     /gene="sdaA"
                     /locus_tag="OO468_002584"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR6259657.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8191..8769)
                     /locus_tag="OO468_002585"
     CDS             complement(8191..8769)
                     /locus_tag="OO468_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR6259658.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(8773..10134)
                     /gene="pabB"
                     /locus_tag="OO468_002586"
     CDS             complement(8773..10134)
                     /gene="pabB"
                     /locus_tag="OO468_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR6259659.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10208..10387
                     /locus_tag="OO468_002587"
     CDS             10208..10387
                     /locus_tag="OO468_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR6259660.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10507..10866)
                     /locus_tag="OO468_002588"
     CDS             complement(10507..10866)
                     /locus_tag="OO468_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR6259661.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11228..11572)
                     /locus_tag="OO468_002589"
     CDS             complement(11228..11572)
                     /locus_tag="OO468_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR6259662.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11704..13614
                     /locus_tag="OO468_002590"
     CDS             11704..13614
                     /locus_tag="OO468_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR6259663.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13672..14367
                     /gene="tsaB"
                     /locus_tag="OO468_002591"
     CDS             13672..14367
                     /gene="tsaB"
                     /locus_tag="OO468_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR6259664.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14407..14988
                     /gene="yeaY"
                     /locus_tag="OO468_002592"
     CDS             14407..14988
                     /gene="yeaY"
                     /locus_tag="OO468_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR6259665.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15194..16879
                     /gene="fadD"
                     /locus_tag="OO468_002593"
     CDS             15194..16879
                     /gene="fadD"
                     /locus_tag="OO468_002593"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR6259666.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            16961..18076
                     /gene="rnd"
                     /locus_tag="OO468_002594"
     CDS             16961..18076
                     /gene="rnd"
                     /locus_tag="OO468_002594"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR6259667.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18130..19095)
                     /gene="yeaX"
                     /locus_tag="OO468_002595"
     CDS             complement(18130..19095)
                     /gene="yeaX"
                     /locus_tag="OO468_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR6259668.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19151..19768)
                     /gene="yeaW"
                     /locus_tag="OO468_002596"
                     /pseudo
     CDS             complement(19151..19768)
                     /gene="yeaW"
                     /locus_tag="OO468_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20110..>20658)
                     /locus_tag="OO468_002597"
     CDS             complement(20110..>20658)
                     /locus_tag="OO468_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6259669.1"
                     /translation="RFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDRL
                     AQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELH
                     SAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKY
                     GIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT         5077 a         5147 c         5343 g         5091 t
ORIGIN      
        1 aacgctatcg atgcgaccac gccggagcaa aggaattgcc agcagggtgg ggccgttacg
       61 gcggggcatg atgaactgat cgtagatcgc gaaggccagg agtgcggcga tgaaaagaat
      121 cagtaccagg tccgtgattg tcattaatcc tccagataaa aaaacggggc caaaaggccc
      181 cggtagtgta cgacagtctt acagtcccag caggccgcaa gcgtaaccag cgataccgat
      241 gacgaagaag ccaacgatga tccacagcgg gttaactttt ttgcgcagca gccacataca
      301 agcaaatgtc agcagcagtg gtacaaggcc tggcattaac tggtcaagaa tagtttgcac
      361 ggtggtgaca cgggtctgtc catcctgccc ggtgatggtc gaaaccacca gcgggatgtt
      421 gacatgtgtc cacttgttaa ccaatgcccc catgacaaac aggccgagga tagacgcccc
      481 ttccgtcagt ttttgcagga agccaccacc catatcttta acgatatcga tacctttgga
      541 gtaaccatac gctacgccgt agtaacgggt tgccagacgc accaggttaa acaggatgaa
      601 gaacagcagc ggacctaaca ggctgccgct catcgcgata ccggcaccca gtgctgcaaa
      661 taccggacgt acggttcccc agaagatcgg gtcgcctaca ccagccagcg gccccatcaa
      721 accgactttg ataccgttga tagcaccgtc atcgatctct gcgccattag cacgctgttc
      781 ttccagcgcc agggttacgc cgagaatcgg tgcagccacg aacggctggg tgttaaagaa
      841 ctccaggtga cggcgaatag cttgtttacg agcttcgttg ttctcagggt agaggcgacg
      901 aattgccggt accatagaga agcagaaacc cagtgcctgc atacgttcga agttccatga
      961 accctggaag aggttagaac gcaggaatac gccacgaata tcactttgag tgagtttttt
     1021 ctcggtggta gtttgagttg tatcaaccat ttcgctcacc tgttagtcca gttcgttatc
     1081 gagatcgttg ttaccagctg cctgagcagg cgcaccggct acgcggttgt atttcgggct
     1141 aagttggatg tagagcactg ccataacagt accaatcaca cccagagcaa ccaggttaaa
     1201 gttggtgaat gctgcggtta cgaagccgag gtagaagaac ggcatcaggt agccagcacg
     1261 catcatgttg ataaccatcg cataaccaac caccacgatc atgcccccgg cgatattcag
     1321 accattggtc accacttccg gaatcgcatt cagcatgttc tgtacttcgc tggtaccaac
     1381 agacagcgca acgatgacgg ccggaatagc cacacgcatt gcttgcagga acagagaaga
     1441 aacgtggatc caggaaatcg ctgtcaggtt gccgttatca gcagccttat ccgcagcgtg
     1501 ctggaaagca acggtaatag tacgaacgat gatggtcagt acctggcccg cagcggccag
     1561 agggattgcc agtgcgatac ctgcaccaat gctctgatga cctgcgataa ccagaatggt
     1621 agaaatgata gaagccagag cggcgtcagg cgcaactgca gcaccgatgt tcatccagcc
     1681 cagcgcgatc atttccagcg taccaccgat aataataccg gttttcatat ccccaagaac
     1741 gatacccacc agggtacacg cgattagcgg acggtgaaac tgaaattcat cgaggattga
     1801 tcccatacct gcgatacagg ctacgataaa taccagcaca atttgaagag tggtaatctc
     1861 cattgtactt ctcctgttta cgtaagactt aagtgtgaaa actgaatgat aatcaacaca
     1921 atacgttact tatcgatttt gctgatcaga tccatcattt tcagtttcgg atcggtggaa
     1981 accttacgga cttccagctc aataccgcgt gcattcagtt ttttgaacgc ctcgatatct
     2041 ttttcatcaa ccgaaaccgc gttattcacc tgggttttac cctgacggaa tgccatacca
     2101 ccgacgttaa cagaggtgat tttcacgccg ccttcaacga gacgctctac atctgttggg
     2161 ttggtaaata acagcattac gcgttcgcca gcatatttcg ggttgttgta gacgcgaatc
     2221 attttggcaa catcaactac gtgtgctgtt acgcccggag gtgcaacctg ggtgagcagt
     2281 gtcttacgaa cggtatccgc agccacttca tcactaacaa caataatacg ggagacattg
     2341 gtttctttgg tccagcgggt ggcgacctga ccgtgaatca gacggtcgtc gatacgcgca
     2401 aggccaataa ccatgtagtc gtttggcccc attggttttg ccggagttgg agccgctttt
     2461 ggtgctgcgg cagcgggtgc tggcgcggct ttttcaaccg gtttggcttt cagtgctttc
     2521 acgccttcac ggcctgtttc tactgccagc gcaaccagtt catcaaagct cgggtcatca
     2581 tcacgggcca ttaacgtttc cacgagcatt ggaatgttaa cgcctgcaat gacttcataa
     2641 tgctctttgt cgacgacaat gcggctggca gcattgaacg ggctgcctcc ccatgtatca
     2701 acgagaaaca gcacgccttt agtggtgtcg agttttgcca actgagcgtt gtatttttca
     2761 atcagcgttt cggcattttc acctggaacg aaatcgatcc agccgacgtt ttcctgctcg
     2821 cctaacagca tttctgccgt tttaagcaac tgctctgcag cccaaccatg tgtgcctata
     2881 acaatagcaa tggtcacttg ctacctcctt tattatcgtt aacacctcaa cgtgccagat
     2941 gtatttttga atcgtcgtcc acaatcgaat cgattcagat aagggctaca accaaaaacc
     3001 cctgcgtttc gcgatttatt ttagatatcg aaaaaattat tttatgtgat gaagatccgt
     3061 aatttaactt tcgattatca gaaatttcga aaggtaaaat atccttgtca cattcgtttg
     3121 caaaggaagg taaatctttg ccaaatcaga ggcgtctctg atatgtttaa ctcccgttta
     3181 acaaccatgg agtatagggc agtagcccgc agtatggatc gtcaccgacg tcatttcagc
     3241 atcaggcctt ttaacgcctg cctttctggc actctatgcc gcacctttcg tttgcatttt
     3301 gtcgttacgc ctgcattatt tctggcgtcg aatagctatt ccttaagcag gagcttgtca
     3361 tggaattctt aatggacccc tcaatttggg cggggctact cacgcttgtt gttctcgaaa
     3421 ttgtgctggg tatcgataac ctggtcttca tcgccattct tgctgacaaa ctgccgccaa
     3481 aacaacgcga taaagcgcgt ttgctggggt tatcactggc gctgattatg cgtctggggc
     3541 tgctgtcgct gatttcatgg atggtcacgc tgaccaaacc gctatttacc gtcatggatt
     3601 tctccttctc cggacgcgat ctgattatgt tgttcggggg gatattcttg ctgttcaaag
     3661 caacaaccga actgcatgaa cggctggaaa accgcgatca tgattccggc cacggtaaag
     3721 gctacgccag tttctgggtg gtcgtcacac agattgtcat ccttgacgcc gtcttctcgt
     3781 tggatgcggt aattactgca gtagggatgg ttaaccatct gccggtgatg atggcggcgg
     3841 tagtgattgc gatggcggtt atgttgctgg catcgaaacc gctgacgcga ttcgttaacc
     3901 agcaccccac ggtggtggtg ctctgtctga gcttcctgtt aatgattggt ctgagtctgg
     3961 tggcagaagg tttcggtttc cacattccga aaggttacct gtatgccgcg attggcttct
     4021 cgatcattat cgaagtgttt aaccagattg cgcgtcgcaa ctttattcgt caccagtcga
     4081 ctttgccgct gcgagcgcgt actgccgatg ccatcctgcg tttgatgggc gggaaacgtc
     4141 aggccaatgt tcagcacgat gccgataacc cgatgccgat cccggaaggt gcatttgccg
     4201 aagaagaacg gtacatgatt aacggcgtac tgacgctggc gtcgcgttct ctgcgcggga
     4261 tcatgacgcc gcgcggtgaa ataagctggg ttgacgctaa tctcggggtc gatgaaatcc
     4321 gcgagcaact gctctcttca ccgcacagtc tgttcccggt atgtcgcggt gaactggatg
     4381 aaatcatcgg tatcgtacgt gctaaagaac tgctggtggc gctggaagag ggcgttgatg
     4441 tggcggcgat tgcttcggcg tctccggcga ttatcgtccc ggaaaccctc gatccgatca
     4501 acctgctggg cgtgctgcgt cgcgctcgcg ggagctttgt tatcgtgacc aacgagtttg
     4561 gtgtggtaca aggcttggtc acgccgctgg atgtgctgga agccattgcg ggtgaattcc
     4621 cggacgctga cgaaacgccg gaaatcatta ccgatggtaa cggctggctg gtaaaaggcg
     4681 gtacagattt gcatgccttg cagcaggcgc ttgatgttga gcaccttgcc gatgacgatg
     4741 atatcgcgac ggtcgcgggc ctcgtgatct cggcaaatgg tcacattccc cgtgtgggcg
     4801 atgtgattga tgtagggcca ctgcatatca ccatctttga agccaatgat tatcgtgttg
     4861 atctggttcg cattgttaaa gagcaaccgg cgcacgatga agatgagtaa gcgttaacgt
     4921 aacgacataa tgggcgtgat atgtccatta tgccgggcgg gcggcggttg gctgcccgcc
     4981 agccatttgg gaaaatcacg tagcggcatc ggctgtgcgt acaaataccc ttgcaaaata
     5041 tgcacgccat gacggcgcaa atacttcgcc tgttcttgcg tttccacgcc ctccgccacc
     5101 agttcaatat tcagtctttg ccccagcgcg atgatgatat cggtcaccgt cgagttaacc
     5161 gcgtcagaac ctatagctgc ggtaaatgac ttatcaattt tcagcacgtc aggacgtaat
     5221 gtttcaagcc aggaaaacga gctgttgccg gtgccgaagt catcaatcgc cagtttgacg
     5281 tttttacgat gcagctcgcg ggcaatccga taatcaacat ccagtaaggc atcgcgttcg
     5341 gtgatttcga ggatcagttg ctgaatcggg tgagcgctaa accagtactg attgagatct
     5401 tttatcagca caccacggcg aaaatggctg ggtgcgacgt taatgccaac atgaaactga
     5461 ctactcatcg ggaaaacatg gtgctgacga atggtttctg ccatcacata gtgggtcagt
     5521 ggcacaatta aatgatgttc ttccgcgata ggaataaaca catccggtga aatccagccc
     5581 tgacgcggat tattccagcg cagcaaaatc tctacaccaa tacattgctg gctgcgcgca
     5641 ttaagcaaag gctgacagaa caattcgaac tcatgttgcg ccaggcccag attaatttcg
     5701 cgggaaaagc tcatccggta agcggtcgcc agccaggcga tatagcccac cagtaaactt
     5761 agcagcaccg ccagcggtaa ttgtgttgga agatagtgcc atgccagcgc cgtagccccc
     5821 ggaccattta cgttaatggt aaaaggaaag ctttgcgaag aaacctgata gttttcattg
     5881 tcttcaggtt gcggaaggga atctaccagc ccattaccat agagcaaatg ccgtttgtcc
     5941 accgtcaggc tggcgctact gatttgcggc agttgtggct caaggagcat tgccgtcagt
     6001 aagtcgatgt taatcatctc tatgacccca gcattgctac tgcccgctgc aggcgtccat
     6061 tgaatcaaaa ccggactgcc tttaatcagg gcacggtcga tcgtcaggcg taaaagtggt
     6121 tgcggtgcag gaagttcagc cagaatgtcc acgacgggca cattgcgata accaaaaatg
     6181 ctggagcaat aaagtgtgcc gtcttgcacc aggccaatgg atcgcacagt ttggagtttt
     6241 gccgcctgtt tacgtaatgg caaatgggcg acagagcagg gcagaccaat cagcggaagc
     6301 aagactttgc tacctgcctg taggggaagc agtactttat ctaattcctc tacagcgtga
     6361 ttggcgaatt gtactacccg ttgttgattt aagttacgct gtgaaataaa tcgcacgctc
     6421 agggtcgaag cgagcgtaac gagtgcgcaa atcgtacaaa caatcattcg attacggcga
     6481 taggttttaa tgatccgttg tgctttttgc atgcgggcca ccctgtaagc gtatggcgac
     6541 aaacgcctga cgcaacagtg gaagtgtagt ggggaatatt acagcagacg agaaagcggg
     6601 tataaattcg cccatccgtt gcagatgggc gagtaagaag tattagtcac actggacttt
     6661 gattgccaga ccaccgcgtg aggtttcgcg gtatttggcg ttcatgtcct taccggtttc
     6721 gtacatcgtt tcgatgacct tatccagcga gacgcgcggt gcactggtgc ggcgcagagc
     6781 catccgtgcg gcgttaatcg ccttcacaga ggcaatggca ttacgctcaa tgcacggcac
     6841 ctgaacctgc cctgcaaccg ggtcgcaggt taaaccaagg ttgtgttcca tgccaatttc
     6901 cgccgccacg caaacctgtt ccgggctacc gcccagcagt tctgcaagac ccgcagcagc
     6961 cattgaacag gcaacaccca cttcgccctg gcaaccaact tccgcaccgg aaatagaggc
     7021 gttcatttta tacaatgcac caatcgcgcc cgctgccata aagtaacggg tatagatgtc
     7081 tgggctgacc gattcaataa agtggtcata gtaagccagc actgccggaa cgataccgca
     7141 ggcaccgttg gttggcgcag ttaccacacg accaccggcg gcgttttctt cgttaactgc
     7201 cagcgcaaac atgtttaccc agtcaatgac attcatcgga tcgttagaca gtttatcgct
     7261 ggaaactaac atccggcgca gggcagacgc acgacgtggc acgcgcagcg ggcctggcag
     7321 tacaccttcg gtgttcatcc cgcgatcgat acatgcctgc atggtttgcc agacatgcgc
     7381 gaaatactcg tcgatctctt tcttgctgtg cagcgccagt tcgttctgca tagcgagacc
     7441 agacagcgaa tagccggttt cattacagta cgcgagcagt tcggtggcag atttgaacgg
     7501 atacggcacg ctcacttcgt tggcagcatc ctgaccaaag tgttcttcat cgacgataaa
     7561 accgccgccg atggaataat aagttttgct gtagacgact tcatcgccgt tataggcgtg
     7621 gatttgcata ccgttttcat gcagcggcag gttgccgtta tgaaaacgca tcccgttgtc
     7681 gcgcgggaaa tccacttcat gccgtccctg tgccagcagc agacgttcgc gctcttttac
     7741 gtcgcgaata aaaccgggaa tactgtcgat atccacggtg gcaggttcgt tacctgcaag
     7801 acccataata atggcgatat cggtgtggtg gcctttaccc gtcagcgaca gtgaaccata
     7861 aacgtccacg gcaacgcgag taacgctatc cagtaagcct ttttcgacca gatcatcgac
     7921 gaactgttta cccgccttca taggccctac ggtatgggaa gatgagggac caatccccac
     7981 cttaaacatg tcgaatagac taatcacgat aatactcctg acaaggtgac tggacttcca
     8041 gtaacgaact aataacagcg catagtgtaa gagggaacgc ggcgactggc ttaactattc
     8101 acatgaatta aactaatcaa aaaccgcgat ttaactaata ttgcgatgat tgtctcaagt
     8161 agtgttttgt agatgtaaag ataagtatag tcagggggtc acaccaattt gcagcgccag
     8221 ctcacgaatt atgcctgcgg tcattcccca tacaaaatac tgttcgtacc aggacagcca
     8281 tacccgatgt gaatcgccac ggcggtagat atctaaaggg tgataacgac ccagatgtaa
     8341 tgcctgggcg agcggcattt caaacaccgc cgagacttca tcttcactgg cgcgatacgg
     8401 cagatcgggc gggataatgc cgaccactgg ggttacttga tagccagtta cgctatcgac
     8461 gggcggcagc acgccgataa cttcaacggc ggaaggcggt atagcgacct cttcttcagc
     8521 ttcgcgcagc gcggcggcga taactgatgc gtccgtgtca tcgactgcac ctccagggaa
     8581 tgccacttgt ccagcgtgtt tacgcagatg aattgaacgc tgagtcagca acaaccccgg
     8641 ttgcggtcga cggacgatgg ggattaacac ggcagcctga cgatgattta ggggttcccg
     8701 gttaatttgc gggcgcaaaa gttgaaagcg agataaaaaa tcatcaagcg tcaggctacg
     8761 gtattccacg tcttacttct ccagttggcg taatatctta ttaactttat caaaagtttc
     8821 ctgatattcc gcttcttcct ggctatcggc gacaattcca ccgcccgcag agcagtatat
     8881 ttgtccgtta atggcagtca gcgtgcggat agtaatgctg gtatccatgt tgccgcaaaa
     8941 gctcaaatag ccaatgctgc cgcaccaggc attacgtcgc tgcggttcca gttcgtcgat
     9001 aatttccata gcccgtactt tcggagcccc ggttattgag ccaccaggaa aagctgcgcg
     9061 cagcagatcg ctggcgtgta actgttctgg tagtcgcgcc gttatagtgc tgaccagatg
     9121 atgcacggca gggaagggtt ccaccacgaa gagctctggt acttttaccg aaccggctac
     9181 ggcaacacga ccgatatcat tacgcattaa atcgacaatc atcagatttt cggcacgatc
     9241 tttcgctgag ttcgccagtt tttctgcttg tttgctatct tcctgaggat cgggcaggcg
     9301 tggtagcgtg cctttaatcg ggcgagtctg gatttcacta ttatcacaaa gaataaaccg
     9361 ctctggcgaa aggcttaaaa ttgcaccctg ttcaagacgt aaaaaagcgc taaatggcgc
     9421 gcggttggcc tgattaagct gaaggaatgc ctgccattca tcgccagaat aggtcgcatg
     9481 aaaacgctgg gcgagattca cctgatagca atcaccgctg tgcagatatt cctgtacctg
     9541 gcgaaatttt tcgccgtact gctcgtgggt catattggat tgccagtcgg aagtgagcgt
     9601 gaaatcttcc tgcggcgaga attgctggct ttccagccag gcccgacggg cattgacatc
     9661 attatgactc agcaaagaaa ctgtatgacg ctggtggtcg acaatgagcg cccaatcgta
     9721 gatacccact gccatatccg gcagaacgat atcttgttcc gcaatttctg gcagtgactc
     9781 aaaacggcgg cccagatcgt agccaaacaa ccccagtgcg ccgccctgaa atggcaaatc
     9841 ttcgttatgc gttgggcgaa tgtctgcgcg atccagcacc tgctggagca cctgtagcgg
     9901 gtcatcagtg gtcgttgtgc gtttttcgct ttcactaaca acggtttctt tacctaaagt
     9961 ggttaaagtg caaatcggat cggcgaccac aatatcaaag cggctatacg gatgatcggc
    10021 atagccggag tgtaaaagca tcgcccacgg caggtggctt aagcgggaga aataaaattc
    10081 agcggcgtcc tgacgccagg gtaaagtaat cacagtggga gataacgtct tcatcagtcc
    10141 tgactctact ggctatgtgc cgggtagcat gaaataatta gcgccaacaa tttagcagga
    10201 gttaacaatg tttgcaggtt taccttcact catccatgaa cagcagcaaa aagctgtcga
    10261 gcggatccag gaactgatgg cacaagggat gagtagcggc caggcaatcg cgctggtggc
    10321 agaagagctg cgtgccaacc actccggcga gctaatcgtg gcgcgttttg aggatgaaga
    10381 cgagtaacac actaatccgt atcgatttcg ttatagaagc cgatgatcat ttgattgtga
    10441 gaaaaaggac gctttttata tgtgattaaa agcgtcctga cccatttatg ctgatataaa
    10501 tttaactcag accagtgctt ttagttcttc ctgcatatat gttgaaaagt attcaggatt
    10561 tttaatcaca gagcgttcac ctgtctccat tttttttgcc catccaacca ttttttccgt
    10621 gaacccaggg ttccagcctt cctggtcagc tcgcgccgta atatcagcgg cactcgcggg
    10681 tactcccagt tcttttaaat atttaaagat tcccttcgcc gtgctttcat tcatcgaact
    10741 tggtgttggc gctgatacat ccatggcacc aatgaaatcc agggctttat ctattacagc
    10801 aggcatattc ttatccttaa aattaaccat gttatcgata aggctatata tgaccttaat
    10861 ttgcatctaa tatcaaaggg attataacga tatggcacga gttaacctgt tgacattcag
    10921 gtgttttaaa ggtagcagct catggtgtgt tatccagtaa gatggagaaa attaacggtc
    10981 agcccgcacg ctataaccag attgttcagc taagaaagaa aaccagagtt gctaatcaaa
    11041 gggcgaacac aacggcatgg ttatgtttat ttatatagtt agttttatag ataaaattga
    11101 aattataaat ataaataatg aattagcaga agcatcaaca gtaatatctt attctctggt
    11161 cgatttatgt aaaaccgacc agtattatcg ataaattatc gtctgactta ttttgcttcg
    11221 ataaagctta caccgcagcc acaatcttaa tttcaacttt atacttcggg ttcatcaaac
    11281 ccgcttgtac cgtgcagcgc accggcgcat gacccgcgac aacccaggca tcccacgctt
    11341 tattcatcgc cgcgaagtcg tttttatcgg ccaggaaaat ggtggcatcc agaatgctcg
    11401 atttattgct gccctgtttt tccagcacgg cgtcaatctg tgccagcgtg ttggcggttt
    11461 gctcaaaggc atcggcgtcg aggttttccg gtacaccagt gtagtagagc gtgttgttgt
    11521 ggattactac atcagaccag cgggcttcag catcgatacg aacgatagtc atcattttgt
    11581 cctcattata cttccatttt cgtgctgcaa agactgccac aatgtgaggt tgccgtcacc
    11641 acttcaactg gcgaaagcgc cctcatcctg acataatccc tgttcaaatc aacagggggt
    11701 agtgtgacgg acgattttgc accagacggt cagctggcga aagcgatacc aggctttaag
    11761 ccgcgagaac cacagcgaca gatggcggta gccgtcaccc aggcgataga aaaaggccag
    11821 ccgctggtgg tggaagcagg aaccggtacg ggcaaaacct acgcttacct ggctcctgcg
    11881 ctgcgggcga aaaagaaagt cattatctcg accggctcaa aagcgttgca ggatcagctc
    11941 tacagccgcg atttgccaac agtctcaaag gcattgaaat atacgggcaa cgtggcgctg
    12001 ctgaaagggc gctcaaacta cctctgcctc gaacgtctcg aacagcaggc gctggcgggg
    12061 ggcgatctgc cggtacaaat cttaagcgat gtgatcctgc tgcgctcctg gtctaatcaa
    12121 acagtcgatg gtgatatcag cacctgcgtc agcgtggcgg aagattcaca ggcgtggccg
    12181 ctggtcacca gcaccaacga caactgtctt ggcagcgact gcccgatgta taaagattgc
    12241 tttgtggtca aagcacgtaa aaaagcgatg gacgccgatg tggtggtggt aaaccatcat
    12301 ctctttctgg cggatatggt ggttaaagag agtggatttg gcgagttgat ccctgaagcg
    12361 gacgtcatga tcttcgacga agcccaccag ctaccggaca ttgccagcca gtattttggt
    12421 cagtcactct ccagtcgaca actgctcgac ctggcaaaag acatcaccat cgcctaccgc
    12481 accgaattaa aagacaccca gcagttacaa aagtgcgctg atcgtcttgc ccagagtgcg
    12541 caggattttc gtctgcaact cggtgagcca ggttatcgcg gtaacctgcg tgagctgtta
    12601 gctaatccgc aaattcagcg ggcattttta ctgctcgatg acaccttgga actttgttat
    12661 gatgtggcga aactgtcact ggggcgttcc gccttgctgg atgcggcatt tgagcgcgcc
    12721 acgttgtatc gcacacggct gaagcggcta aaagagatca atcagccggg ctacagctac
    12781 tggtacgaat gcacttcgcg ccattttact ctggctctca cgccgctcag cgtggcggat
    12841 aaattcaaag agttaatggc gcaaaagccc ggtagctgga tcttcacctc agcaacgctg
    12901 tcggtgaacg acgatctgca tcatttcacc tcgcggcttg ggatagaaca ggcggagtcg
    12961 ttgctattgc caagcccgtt tgattacagc cgccaggcgt tactctgtgt gccgcgcaat
    13021 ctgccgcaaa ccaaccagcc aggttctgct cgccagttag cggcaatgct gcgaccgatc
    13081 atcgaagcta acaacggtcg ttgttttatg ctttgtacct cgcacgccat gatgcgcgat
    13141 ctggccgagc agttccgcgc taccatgacg cttcccgttt tgttgcaggg ggaaaccagt
    13201 aaaggccaac tgttgcagca gtttgtcagt gccggtaatg cgcttcttgt ggcaaccagt
    13261 agtttctggg aaggggtgga cgtgcgtggc gatacattgt cattggtaat tatcgacaaa
    13321 ttgccgttta cctcgccgga tgatccactg ttaaaagcgc gcatggaaga ttgtcgtttg
    13381 cgcggtggcg acccgttcga tgaagtgcaa ctaccagatg ccgtcattac tctcaaacag
    13441 ggggtaggtc gactgattcg cgacgccgac gatcgtggcg tgctggtgat ttgtgactat
    13501 cggctggtga tgcgtcctta cggcgcgacg tttctcgcca gtctgccgcc cgcgccacgc
    13561 acccgtgaca ttgcccgtgc ggttcgtttc cttgcgatac catcctccag gtaatttgtt
    13621 acggagtatg gtatgatgcg cgccttttta ttgatctaag cacgaagacc catgcgaatt
    13681 ctggctatcg ataccgcgac agaggcctgc tctgtcgccc tgtggaacga cggtactgtc
    13741 aacgctcatt ttgagctttg ccctcgtgaa catactcaac gaatcttacc gatggtgcag
    13801 gatatcctga ccaccagcgg aacttccctg actgatatta acgctctggc ttacgggcgc
    13861 ggccccggta gctttaccgg cgtgcgcatt ggtattggca tcgcgcaagg gctggcgctt
    13921 ggcgcggaat taccgatgat tggcgtctcc acgctaatga cgatggcgca aggggcgtgg
    13981 cgcaaaaacg gcgcaacacg cgtgctgtca gccattgatg cgcgaatggg cgaagtttac
    14041 tgggccgaat atcagcgtga tgaaaacggt atctggcacg gtgaagaaac cgaagccgta
    14101 ctcaaacccg aactcgttca tgaacgaatg caacagcttt ccggcgaatg ggtgacggta
    14161 gggacgggct ggcaagcctg gccggatctc ggtaaagaga gcgggctggt tttgcgcgat
    14221 ggcgaagtgt tactccccgc tgcagaagat atgctgccga ttgcttgtca gatgtttgcc
    14281 gagggtaaaa cggtggcggt ggaacatgcc gagccggttt atttgcgtaa caacgtcgca
    14341 tggaagaaac ttccgggcaa agaatgaatc ttaagagtat gccctgagaa aaaaaggagt
    14401 cgcatcatgg cggttcaaaa gaatgttatc aaaggcatac tggcaggtac gtttgcgcta
    14461 atgctgagcg gttgtgtcac tgtgccggac gccattaaag gcagcagtac cacgccgcaa
    14521 caagatttag ttcgggtgat gagtgcgccg cagctgtacg ttggtcagga ggcacgcttt
    14581 ggtggcaaag tggttgcggt acaaaaccag caagggaaaa cccgcctgga aattgctacc
    14641 gtaccgctgg acagcggagc taggccgacg ctgggagaac cttctcgcgg tcgcatttat
    14701 gccgatgtga acggttttct ggacccggtg gatttccgtg gacaactggt tacggtagtc
    14761 gggccaatca ccggtgcggt tgacggcaaa atcggcaata cgccctataa atttatggtg
    14821 atgcaagtaa cgggttacaa acgttggcat ttaacccagc aggtgattat gccgcctcag
    14881 ccgattgatc catggtttta tggcggtcgt ggctggccct atggctacgg cggatggggc
    14941 tggtataatc ccggccccgc gagagtacaa acagttgtaa ccgaataatt gcttgttttt
    15001 aaagaatgca gaaacagcgg ctggtccgct gtttctgcat tcttacggta aagataaaaa
    15061 taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt atcaagctgg tatgatgagt
    15121 taatattatg ttaacggcgt gtatatcatt tggggttgcg atgacgacga acacgcattt
    15181 tagaggtgaa gaattgaaga aggtttggct taaccgttat cccgcggacg ttccgacgga
    15241 gatcaaccct gaccgttatc aatctctggt agatatgttt gagcagtcgg tcgcgcgcta
    15301 cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg accttccgca agctggaaga
    15361 acgcagtcgc gcgtttgccg cttatttgca acaagggttg gggctgaaga aaggcgatcg
    15421 cgttgcgttg atgatgccta acttattgca atatccggtg gcgctatttg gcattttgcg
    15481 tgccgggata atcgtcgtaa acgttaaccc gttgtatacc ccgcgtgagc ttgagcatca
    15541 gcttaacgat agcggcgcat cggcgattgt tatcgtgtct aactttgccc acactctgga
    15601 aaaagtggtt gataaaaccg ccgttcagca cgtaattctg acccgtatgg gcgatcagct
    15661 atctacggca aaaggcacgg tagtcaattt cgttgttaaa tacatcaagc gcttggtgcc
    15721 gaaataccat ctgccagatg ccatttcatt tcgtagcgca ctgcataacg gctaccggat
    15781 gcagtacgtc aaacccgaac tggtgccgga agatttagcc ttcctgcaat acaccggcgg
    15841 caccacaggt gtggcgaaag gcgcgatgct gactcaccgc aatatgctgg cgaacctgga
    15901 acaggtcaac gccacctatg ggccgttgct gcatccaggt aaagaactgg tagtaacggc
    15961 actgccgctg tatcacattt ttgccctgac cattaactgc ctgctgttta tcgaactggg
    16021 tgggcagaac ctgcttatca ctaacccgcg tgatattcca gggttggtaa aagagttagc
    16081 gaaatatccg tttaccgcta tcactggcgt taacaccttg ttcaatgcgt tgctgaacaa
    16141 taaagagttc cagcagctgg atttctccag tctgcatctt tccgcaggcg gtgggatgcc
    16201 tgttcagcaa gtggtggcag aacgctgggt gaaactgacc ggacagtatc tgctggaagg
    16261 ttatggcctg accgagtgcg cgccgctggt gagcgttaac ccgtatgata ttgattatca
    16321 tagcggtagc atcggtttgc cggtgccatc gacggaagcc aaactggtgg atgacgacga
    16381 caacgaagta ccaccgggtc aaccgggtga gctttgtgtc aaaggaccgc aggtgatgct
    16441 gggttactgg cagcgtccgg atgctacaga tgagatcatc aaaaatggct ggttacacac
    16501 cggcgacatc gcggtgatgg atgaagaagg attcctgcgc attgtcgatc gtaaaaaaga
    16561 catgattttg gtttccggtt ttaacgttta tcccaacgag attgaagatg tcgtcatgca
    16621 gcatcctggc gtacaggaag tcgcggctgt tggcgtacct tccggctcca gtggtgaagc
    16681 ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc gaagagtcac tggtgacttt
    16741 ttgccgccgt cagctcacgg gatacaaagt accgaagctg gtggagtttc gtgatgagtt
    16801 accgaaatct aacgtcggaa aaattttgcg acgagaatta cgtgacgaag cgcgcggcaa
    16861 agtggacaat aaagcctgag cgttaagtca gtcgtcagac gccggttaat ccggcgtttt
    16921 ttttgacgcc cactaaagag aaaacaattt gaattaccaa atgattacca cggacgatgc
    16981 gctggcttct ttgtgtgaag ccgtccgtgt ctttccggcg atagccctgg atactgaatt
    17041 tgttcgtacg cgcacttatt acccgcagct ggggttgatt caacttttcg atggcgagca
    17101 tctggcgcta attgatcctc tcgggatcac cgactggtca ccgctgaaag cgatcctgcg
    17161 cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa gatctggaag tgttcctcaa
    17221 tgtctttggc gaattaccac aacccttgat tgacacgcaa atccttgctg ccttctgcgg
    17281 acgcccgatg tcatggggtt tcgcttccat ggtggaagag tattccggcg ttacgctgga
    17341 caagagtgaa tcgcgcaccg actggctggc cagaccgctg accgaacgtc agtgtgaata
    17401 cgcagcggcg gatgtctggt atctgttacc gatcaccgcc aagcttatgg tagaaacgga
    17461 ggcctccggc tggctacctg cggcgctgga tgaatgccgc ctgatgcaaa tgcgtcgtca
    17521 ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc aatgcctggc agttacgcac
    17581 acgccaactg gcctgtctgc aactgttagc cgactggcga ctgcgcaagg cgcgagagcg
    17641 tgatctggcg gtgaactttg tcgtgcgtga agagcatttg tggtcggtcg cgcgttatat
    17701 gccgggaagt ttaggcgaac tggacagcct gggtttatcc ggtagcgaaa tccgctttca
    17761 cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca ttgccggaag atgccttacc
    17821 gcagccgatg cttaacctga tggacatgcc tggttatcgt aaagcgttta aagcgattaa
    17881 gtcgctgatt actgacgtga gcgaaacgca taagatcagc gccgaattgc tggcatcgcg
    17941 tcggcaaatc aaccaactgc tgaactggca ctggaaactg aaaccgcaga acaatttgcc
    18001 ggagctgatt tccggctggc gtggtgagct gatggcggaa gcattacaca atttattgca
    18061 ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg cgctcagcgc cacatccggc
    18121 tttggcaaac tacaaatcca acaccaggcg tttacccttc gcacgcgaac aacagatcaa
    18181 catactttgc tggctggcac gctcttcatc gctaaaatac tgatcccgat ggtcagcttc
    18241 accttccagt attgccgttt cgcaggttcc gcatacccct tcacgacata aacattccac
    18301 tttcgcggct ttattatttt caataacctg caaaatagtc atctcttccg gcaccacaaa
    18361 ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg ccggttttgt cttcgatagc
    18421 aaattgctca aagtgcagtg tatcggcggc gatgtccaga cgcgcagctt cacttcttac
    18481 cgcttcaatt agcgcctcgg ggccacaggt ataaacgtgt gtgccaggtt cgatatccgc
    18541 caataatcgc gctaattcca gtcgtgttcc ggttgatgaa tgatgcaaat ggactttctc
    18601 agcctgcgga tgctggacta gctcagcacg aaatgcgcaa ctttctggat ttcgcgagca
    18661 gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct gccatgtgcg acaggaaagg
    18721 ggtgatacca ataccgccca cgataaacag atgctttctg gctgagggaa ttagcgcaaa
    18781 caggttatta ggcgttgaga tcgttaaccg atcgcccact tttacctgct gatgcaaaaa
    18841 gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca atctgataac tagaggtgtt
    18901 atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg ttatcaccat cgctcatctg
    18961 cactaggatg tgacttcctc cggtgaacgc aggcagcggt ttgccatcgg ttgccaccag
    19021 cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg ctcacctgta cttcaaacat
    19081 ttgatagtct gacattgctg tactccatca aaaatcagcc cagcggcgga aaataccgcc
    19141 gccgatgtca ttagtcctta aacacctgcg ccagcagatt atggaaatgg gcgataccat
    19201 gttcggaaat gccactaccg ctactgtcgg ccatgatgcg cccctgacca cgatagccac
    19261 gcgatttcag ccctttctgt acgctttcaa ccagacgtaa atcttccgga tggaacacat
    19321 cgcgatacca ctcaatcagc gatttttgct cgtcggttaa ctcttcattg gtgaagtaaa
    19381 tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa ttcataaatg accgtcatca
    19441 tccctttgat cggggtgacg ttcagcatcg tgcacggcca cagccagaaa ccgtggaatg
    19501 ccgcatccgt accctcttca aatttaaacg actgttcgga cggtttggcg aaaccgtatt
    19561 gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc aacctgtacg gagtcggaga
    19621 aacctagatg cgccggacca cagtgatagc actcgagata gttatcgaca atgttcttcc
    19681 agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt cagattgtgg acttccgggc
    19741 aggcttccag cactttcgcg cccagaccgg tgtattgatt cacttgaagt acgaaaaaaa
    19801 accgggagga cattggatta ttcgggttct gatgggatta gatttggtgg ggcttgcaag
    19861 cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga aagatagtaa aaaattgctt
    19921 ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat catttcaata acagcttcaa
    19981 aaaacgttca aaaccgataa caaccaggct gtaaccaaat gactcatatc acaaatcagc
    20041 tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg tgaagtatgt tttagctggt
    20101 tcatggttgt tatacggctt tttttacatc ttgtggttct tgtgaaggag ctacaagacc
    20161 ttcctgttca taaataccat actttgacaa aatcttttta cgagtatctg ttggtaatga
    20221 ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata ccttgcaaat cagcctggat
    20281 ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca aatcctttct ttctgaacaa
    20341 ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg acaattgcga tcagtttagt
    20401 tttcaatgtg ctgcggtcag gttgagaatt tttattctct tgattatttt tatgctcaac
    20461 cattggcatg ctctgacttt ggataaacca ctctattgga cgccctgttg cgtaggctat
    20521 ttgtgctaat cgatctaaag acgggtatgt cttgccagac aggtagtttc tgataacccc
    20581 atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat tctccattca ttgcctcttc
    20641 aaggcgttcc ttgaaacg
//
LOCUS       DAJTUP010000068        20571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTUP010000068 DAJTUP010000000
VERSION     DAJTUP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.068"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..460
                     /locus_tag="OO468_002598"
     CDS             <1..460
                     /locus_tag="OO468_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR6259670.1"
                     /translation="FAVNGRKLYLSPVIDLFNNEVISYSLSERPVMNMVENMLDQAFK
                     KLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLDNAVVECFFGTLKSE
                     CFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLTPIEYRNQTYMPRV"
     gene            562..714
                     /gene="hokA"
                     /locus_tag="OO468_002599"
     CDS             562..714
                     /gene="hokA"
                     /locus_tag="OO468_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR6259671.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(901..1113)
                     /gene="cspA"
                     /locus_tag="OO468_002600"
     CDS             complement(901..1113)
                     /gene="cspA"
                     /locus_tag="OO468_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR6259672.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1394..1684)
                     /locus_tag="OO468_002601"
                     /pseudo
     CDS             complement(1394..1684)
                     /locus_tag="OO468_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2118..2828
                     /locus_tag="OO468_002602"
     CDS             2118..2828
                     /locus_tag="OO468_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR6259673.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2878..3852)
                     /gene="ghrB"
                     /locus_tag="OO468_002603"
     CDS             complement(2878..3852)
                     /gene="ghrB"
                     /locus_tag="OO468_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR6259674.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3956..4606)
                     /locus_tag="OO468_002604"
     CDS             complement(3956..4606)
                     /locus_tag="OO468_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR6259675.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4769..6967
                     /locus_tag="OO468_002605"
     CDS             4769..6967
                     /locus_tag="OO468_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR6259676.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6936..7376)
                     /locus_tag="OO468_002606"
     CDS             complement(6936..7376)
                     /locus_tag="OO468_002606"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR6259677.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7373..7936)
                     /gene="tag"
                     /locus_tag="OO468_002607"
     CDS             complement(7373..7936)
                     /gene="tag"
                     /locus_tag="OO468_002607"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR6259678.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8094..8792
                     /locus_tag="OO468_002608"
     CDS             8094..8792
                     /locus_tag="OO468_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6259679.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9021..10229
                     /locus_tag="OO468_002609"
     CDS             9021..10229
                     /locus_tag="OO468_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259680.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10553..12244
                     /gene="eptB"
                     /locus_tag="OO468_002610"
     CDS             10553..12244
                     /gene="eptB"
                     /locus_tag="OO468_002610"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR6259681.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12336..12412
                     /locus_tag="OO468_002611"
     tRNA            12336..12412
                     /locus_tag="OO468_002611"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12370..12372,aa:Pro,seq:cgg)
     gene            13156..14763
                     /gene="dppA"
                     /locus_tag="OO468_002612"
     CDS             13156..14763
                     /gene="dppA"
                     /locus_tag="OO468_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR6259682.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15071..16090
                     /gene="dppB"
                     /locus_tag="OO468_002613"
     CDS             15071..16090
                     /gene="dppB"
                     /locus_tag="OO468_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR6259683.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16100..17002
                     /gene="dppC"
                     /locus_tag="OO468_002614"
     CDS             16100..17002
                     /gene="dppC"
                     /locus_tag="OO468_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR6259684.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17013..17996
                     /gene="dppD"
                     /locus_tag="OO468_002615"
     CDS             17013..17996
                     /gene="dppD"
                     /locus_tag="OO468_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR6259685.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17993..18997
                     /gene="dppF"
                     /locus_tag="OO468_002616"
     CDS             17993..18997
                     /gene="dppF"
                     /locus_tag="OO468_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR6259686.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19027..20298)
                     /locus_tag="OO468_002617"
     CDS             complement(19027..20298)
                     /locus_tag="OO468_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6259687.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         4967 a         5059 c         5380 g         5165 t
ORIGIN      
        1 atttgcagtc aatgggcgca agctgtattt gtctccagta atagatctct tcaacaacga
       61 agttatttct tacagccttt cggaaagacc agtgatgaac atggttgaga atatgctcga
      121 tcaggcattc aaaaagctta atcctcacga gcatcctgtt ctgcactctg accagggatg
      181 gcagtatcgt atgagaagat atcaaaatat ccttaaagaa catggtatta aacaaagcat
      241 gtccagaaaa ggcaattgtc tggataatgc tgtggtggag tgtttctttg gaaccttaaa
      301 gtcggagtgt ttttatcttg atgagttcag taatataagc gaactgaagg atgctgttac
      361 ggaatatatt gaatactata acagcagaag aattagcctg aaattaaaag gtctgactcc
      421 aattgaatat cggaatcaga cctatatgcc tcgtgtataa ctgtccaact ttttggggtc
      481 agtacaaagt gaggctaccc tatgcctgaa caccataagg ttagcctctt actcgttgga
      541 aatcaacaca gggggctggg aatgccgcag aaatatagat tactttcttt aatagtgatt
      601 tgtttcacgc ttttattttt cacctggatg ataagagatt cactgtgtga attgcatatt
      661 aaacaggaga gttatgagct ggcggcgttt ttagcctaca aattgaaaga gtaagagtct
      721 tcgacgggaa attattcccg ccttacttac ggcgttgcgc attctcattg cacccatatt
      781 tattcttcac aaaaataata atagatttta ttacgcgatc gattatttat ttcctgaaaa
      841 aaaataaaaa aatccccgcc aaatggcagg gatcttagat tctgtgcttt taagcagaga
      901 ttacaggctg gttacgttac cagctgccgg gcctttagcg ccgctttcga tggtgaagga
      961 cactttctga ccttcgtcca gagatttgta accatcgttc tggatagcag agaagtgtac
     1021 gaacacatct ttagagccat cgtcaggagt gatgaagccg aagcctttgt cagcgttgaa
     1081 ccattttacg ataccagtca ttttaccgga catagtgtat tacctttaat aattaagtgt
     1141 gcctttcggc gatatggcgt gctttacaga ttttgaagcg ttaaaggaat gtgcactacg
     1201 aggggtatca acgataactc ttgaagggac ttgccttact acactgcttt aatggtctgt
     1261 acgtcaaacc gttgatgtgc attaagccac tcattggcgg gtgatgcaac aattattttt
     1321 catatttatg attaatcggt tggaaaacgg tcctgtcatc aggaccgtaa acagcaataa
     1381 agtggataaa agtctatttc atcaactgct tacttaatgc cgggttggct tgaatcaaac
     1441 gcatcaattt tagttcggca cttgaaggct tcacgcgtct ggattcccat tcctttacca
     1501 tggcgactga tacgcccaaa acccgggcga aatcatcgat ttttaatcct gtcccttttc
     1561 ataactgctc aatttcggta caggacgttg ttctgtgcgt cagggtaatt ttctgcgttt
     1621 catctttaaa aacaatctgt tccaggctgc tcaacagctc atgcattgga tctttatatt
     1681 ccattgaaaa ctcctcaaat cacactgcgg gatcgtgaat tgcatcgaag ctcattaaga
     1741 atagtcggga aaagcagaca cagcggggtg aagtcagggc atgctctggc tatgattcga
     1801 ttcagcctgt catttagcgt gacttatagc cacctaacga tctgataaat gccgatatat
     1861 ctcttatcac tcctgttttg taaagatttc gcaaatctaa ccttctgcga aggcgtaaat
     1921 ctctggctac aaaaaaggca acggcactat cattttttaa caatgaaaca acaaattaac
     1981 cttgtttcat tttctccatg attttaccag gaagattacg tagaaagggt atcctgcaag
     2041 cctgtcctgg actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg
     2101 gtgaaaggag taagaaaatg gcgacaggaa agtcctgctc tcgctggttt gcgcctcttg
     2161 cggcgttatt aatggtagtt agcctgagtg ggtgttttga taaagaaggc gatcagcgta
     2221 aagcgtttat cgacttcctg caaaatacag tgatgcgtag cggtgaacgt ctaccaaccc
     2281 tgactgccga tcagaaaaaa cagtttggtc cttttgtctc tgattacgcg attctgtatg
     2341 gttattctca gcaggtgaat caggcgatgg attccggtct gcgcccggtc gtagacagcg
     2401 ttaacgcaat tcgcgtaccg caggattacg ttacgcaaag cggtccgctg cgtgaaatga
     2461 atggttcgct gggtgtgctg gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg
     2521 cgcactctgc gctaaaacag agcgatgacc tgaaaccggt atttgatcag gcattcacca
     2581 aagtggtaac gacgccagct gatgcattgc aaccgttgat tccggcggca caaactttca
     2641 cgcaacaact ggtgatggtt ggagactaca ttgctcagca aggtactcag gtgagctttg
     2701 tcgcaaatgg catccagttc ccgacttcac agcaggcaag tgaatataac aaactgattg
     2761 cgccattacc ggcacaacat caggcgttta atcaggcctg gactacagca gttactgcaa
     2821 cccaataaag agtaaaagcc cgagcgatta tcgggcttta cttcacgcag tcgcagctta
     2881 gtccgcgacg tgaggattca cacagttctt ctcaaccttt ccttgtaacg catcaatcaa
     2941 attatccacg gcacaggcgg ccatgccata acgcgtctca tgggtggcag atccaatatg
     3001 cggtactgcg acgacgttgg ccattgagag caacggcgaa tctacggaca gtggctcttg
     3061 ttcgaagaca tccagcccgg cggcgtgaat ttcgcctttc tgcaatgctg cgatcagtgc
     3121 attttcgtca accaccgggc cacgtccggc attaatgaaa atggcggagg atttcatttt
     3181 ggcgaattgt tctgcgccaa acagatgatg cgtctcatca gttaacggca ggatcaggca
     3241 aacgaaatct gactcttgta acagagtatc caaatcgcag tagcgggcgt tgaagcgttc
     3301 ttctgcttct ttatggtggc ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg
     3361 cacacgttgt gccagcgcca tgccgatccg tcccatcccg acaatgccca gtgttttatg
     3421 gtgaacgtca gtgccgtacc agtccgggcc tatgctcgcg gtccattcgc ctgcttttac
     3481 ccgttctgct acctccacaa cccgacgagc ggtagacaac accagcgcca tcagcgtatc
     3541 ggcgacggtt tctgttaata cggttggcgt gtgcatcagc agaatttttc gggcggtaag
     3601 cgcatcgaca tcaaaattgt catagccgac ggagatcgtt gatgtggcac gcagtttcgg
     3661 cattttttcc agcaatgcgg catctacatt ctcgtttgaa cccagtaaac cttcagcttc
     3721 tgcaaaaatt gctgcatttt gaacgacggt ttgtgggctg aggtttgcca cctggtgaac
     3781 ggtgaaatgc gcttgcaggc gttgcagtaa atcatcaggt aaggctttgt agaggataac
     3841 ggacggcttc atgcttctct ccattcactg ataaggcgat caccttagcc tgaaatatga
     3901 tgaaggaaaa ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa agggattaca
     3961 gcgggcttaa ggtaatttct acacggcggt tttgcgcctt accttctgcg gtgctgttgc
     4021 tggcgattgg gttagccggg ccaaggccct gagtacggat gcggctggcg tccacgccct
     4081 gggtgatcaa cgcgctggca acggaatccg cacgctgctg ggagagacgc atgttcaggt
     4141 cgtgaccccc cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc ggatactctt
     4201 tcagcaccat tgccacgccg gtcagggtgt tagcgcctgc cggtttcagg ggagcgctgc
     4261 tgctgtcgaa ggtcacattg ttcggcatat tgaggataat gttatccccg ctgcgggtta
     4321 cgctaacacc agtgccgcgc attttgtcac gcagcttcgc ttcctgcaca tccatgtaat
     4381 aacccacgcc gccgcccaga gctgcgcctg ctgctgcgcc aatcagcgcg cctttaccgc
     4441 gatctttctt cgaagaagag agcgcaccaa tacccgcgcc cacgagagag cccagacctg
     4501 cgccgatagc agatttgcct gcttcgcgtt cgccggtgta agggttagtt gtgcagccag
     4561 ataccgccag agcgccactc actacggcgg caagtaagat aaacacgttt cttcattgtt
     4621 aatccttaat aaccttttta ttctttgtca cgggttccgt ggcgggagat tatgccgcgt
     4681 gaacatgaag attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa
     4741 tacatctaac ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct
     4801 gactgccgcc cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc
     4861 gcgtggcgcg ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt
     4921 tcacagcaat acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga
     4981 aaacccgcaa ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct
     5041 ggcaggcggt tataccgggc atctggggga ttattcgacc ggtgcggcac aggcgatcat
     5101 gccgtatgtc gtaggtggca gcgaagttta tcaacagcaa accagttggc cgctggtgct
     5161 ggaacatagc gatgtcgtgg tgttgtggag tgctaaccca ctcaatacgc tgaaaattgc
     5221 gtggaatgca tccgatgagc aggggctttc ttacttttcc gcactgcgtg acagtgggaa
     5281 aaagctgatc tgcattgatc caatgcgatc ggaaaccgtc gatttctttg gcgataaaat
     5341 ggagtgggtg gcaccgcaca tgggcaccga tgttgcgctg atgctgggga tcgcccatac
     5401 gctggtggaa aatggttggc acgacgaagc gtttctggcg cgttgcacca caggttatgc
     5461 cgtcttcgcc tcttatttgc tgggcgagag tgacggaata gcgaaaaacg ccgaatgggc
     5521 ggcagagatt tgtggtgttg gcgcagcgaa aatccgcgag ctggcggcta ttttccacca
     5581 aaataccacc atgctgatgg caggctgggg aatgcagcgc caacagtttg gtgagcaaaa
     5641 acactggatg atcgtcacgc tggcagcaat gttggggcaa atcggcacac ccggcggcgg
     5701 ttttggtctt tcttaccatt ttgccaatgg tggtgacccc acgcggcgtt ctgcggtgct
     5761 ctcttccatg cagggcagct tgccgggtgg ctgcgatgcg gtggataaaa tccctgttgc
     5821 ccgcattgtt gaagcactgg aaaaccctgg tggcgcatat caacacaacg gtatgaaccg
     5881 acatttcccg gatattcgtt ttatctggtg ggcgggcggt gccaacttta ctcatcatca
     5941 ggataccaat cgtctgatcc gtgcctggca aaaaccggag ctggtggtga tctctgaatg
     6001 cttctggacg gcggcggcga aacacgcgga tattgttctg cctgcgacta cctcttttga
     6061 gcgtaatgat ctcaccatga ccggtgatta cagtaatcag catctggtgc cgatgaagca
     6121 agtggtgccg ccacgctatg aagcgcgtaa tgattttgat gtttttgccg agttgagtga
     6181 acgttgggag aagggcggct atgcgcggtt tacggaagga aaaagtcagc tgcaatggct
     6241 ggaaacgttt tataacgttg cccggcagcg cggggcaagc cagcaggttg aattgccgcc
     6301 atttgctgag ttctggcaag ccaaccagtt aattgagatg ccggaaaacc cggacagcga
     6361 gcggtttatt cgcttcgctg atttttgccg cgatccgctg gcgcatccgt taaaaaccgc
     6421 cagcggcaag attgaaatct tctcacagcg tattgccgat tacggttacc cggattgccc
     6481 tgggcatcca atgtggctgg agccggacga atggcagggc aatgccgaac cagaacagtt
     6541 gcaggtactt tctgcccatc cggcgcaccg cctgcacagc cagctgaatt acagttctct
     6601 gcgcgaattg tacgcggtag caaaccgtga gcctgtcacc attcatcctg acgatgctca
     6661 ggcgcgcggc ataaccgaag gggatatggt gcgggtgtgg aactcccgtg ggcagatcct
     6721 tgccggagcg gtcattagcg agggaattaa acctggcgtg atttgcattc acgaaggggc
     6781 atggccggat ctggatttaa ccgctgacgg tatttgtaaa aacggcgcag tgaacgtgct
     6841 gaccaaagat ctccccagct cgcggctggg gaatggctgt gcgggtaata cgacgctggc
     6901 atggctggaa aaatacaacg gtccggaact gacacttaca gcgtttgaac caccggccag
     6961 ctcataatcc aggtgggtag ttgggtttca tcctgccatg cgcaatcgac aatgtgaaaa
     7021 ccctgcgcac ggtaaaaatc tatcgccggt tgattttttt gataaacctc cagcatcaga
     7081 tggggatagc gctgctgcac atactgcatc agcgccttac caataccgcg tctgacggcc
     7141 ttcggtgcga caaacattgc tgccagaaat cggccttcca taatgctgac aaaaccgaga
     7201 agcttaccgt cctcttccca gacccagttt tgcgcgttgg caagataggc atcccgcacc
     7261 agcggaatgc agtcacgcca gtaattcgct tttataaagg gatgccccca ggttgtactt
     7321 tccagccaca gttcgaggat cgcggggagt tctgaacgtt gcgcttcccg aatcatggtt
     7381 tatttcccag atagcaacag cagccaacca catgatcatt caccagccca catgcctgca
     7441 taaaggagta acagattgtg gtgccgacaa acttaaaacc acgttttttc agtgccttag
     7501 atagggcgtc ggaggcggac gtagatgtgg gaatttcgct caacgttgtg gcttgtgtca
     7561 cctgtggctg atgatttaca aacgaccaga caaagtcggg aaacggttcg ccgttctgtt
     7621 ccatttgcag gtacgcccgc gcattaccaa taattgcctg aattttccct cgatggcgga
     7681 taatcccggc gtcctgtacc agtctttcga catcctcttc ctgcattgct gcgaccttca
     7741 ccggatcgaa ctgatgaaag taggcgcgat agttttcgcg ttttttgagg acggtgatcc
     7801 acgataaccc agcctgctgc ccttcaaagc agatcatttc gaacagtttt ttactgtcag
     7861 tttcaggcac gccccactca ttatcatggt aggcaatata aagcgggccc tgactcaccc
     7921 agccgcaacg ttccatactt tccctcgcta tgtacccgat cgtaaaaatt tcagccgctt
     7981 ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg gctactatgc ccgatatcct
     8041 tcatacaatg acaataatat tgccgaattc ggctcttccc tggagtcgtg ttaatgatca
     8101 taaaaaaaag cggtggtcgc tggcagctaa gcctgctggc gagcgtggta atcagtgcct
     8161 tttttctcaa cacagcttac gcctggcaac aagaatatat cgttgatacg caacccggac
     8221 attccacaga gcgttacacc tgggatagtg atcatcaacc tgattacaac gatattttgt
     8281 cgcaacgtat tcaaagtagc caaagggcgc tgggactgga agtcaatctg gcggaagaaa
     8341 ctcctgtgga tgtgaccagc agtatgagta tgggctggaa ttttccttta tatgaacagg
     8401 ttacaaccgg cccggtcgcg gcattacatt acgatggcac aaccacctcg atgtataacg
     8461 agtttggcga cagtactacc acgctgaccg atccgttatg gcatgccagc gtgagtagct
     8521 taggctggcg tgttgactcc cggcttggcg atctccgacc ctgggcgcaa atcagctata
     8581 accagcaatt tggcgagaat atctggaagg cgcaatcagg cctgagccgg atgacggcga
     8641 caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc tgatatgttg ctcaatcaaa
     8701 atattgccgc ctatgctgcg ctaactcagg cagaaaatac cactaataat agcgactatc
     8761 tgtatacgat gggggttagc gccagatttt aacgtaacag tcacaattga aaccattgaa
     8821 taacaacagt tgtggcgata gtgggtgcta acttaccaaa taataaattt ggtgaataat
     8881 tgtcgcgtca ttcattcctg aactaaggca tttcattccg ttctgatggc atttcatgcc
     8941 gtttttcccc aagcataaaa tgcacttcgt tatggttgtc ggcagagatt tttccttttt
     9001 attactgcag gaatactgcc atgacacctt caaattatca gcgtacccgc tggctgacac
     9061 tcatcggtac tatcattacc cagtttgcgc tggggtcggt ttatacccgg agcctgttta
     9121 atggcgcgct ttctgccaag ctggatgcgc cggtaagcca ggtcgctttc tctttcggct
     9181 tgttaagtct ggggctggca atttcgtctt ctgttgcggg caaattgcag gaacgttttg
     9241 gcgttaaacg cgtcaccgtg gcttccggca ttttgctggg attaggcttc ttcctgaccg
     9301 cgcattccaa caacctgatg atgctgtggt taagcgccgg tgtgctggtc ggtctggcgg
     9361 atggcgcggg ttacctgctg acgctctcta actgcgtgaa gtggttcccg gagcgtaaag
     9421 gtctgatctc cgcgttcgct atcggttctt atggtctggg tagcctgggt ttcaaattta
     9481 tcgacacgca gctgctcgaa acggtgggtc tggaaaaaac ctttgtgatt tggggagcga
     9541 ttgcgctggt gatgattgtt ttcggcgcaa cgttaatgaa agacgcacca aaacaggaag
     9601 tgaaaaccag caatggtgtg gtggagaaag attacacgct ggcagagtcg atgcgtaaac
     9661 cgcagtactg gatgttagcg gtaatgttcc tgactgcctg catgagcggc ctgtacgtga
     9721 ttggggtagc gaaagatatc gcccaaagtc tggcacacct tgatgtggtt tccgcagcca
     9781 atgcagtaac ggttatttcc atcgctaacc tttcaggtcg tctggtgctg ggcattctgt
     9841 ctgacaaaat tgcccgtatc cgtgttatca ccattggtca ggtgatttcg ctggtgggta
     9901 tggcggccct gctgtttgca ccattaaatg cagtgacgtt ctttgcagcg attgcctgcg
     9961 tggcatttaa ctttggcggc actattaccg tctttccatc actggtcagt gagttcttcg
    10021 gcctcaataa cctggcgaaa aactacggtg tgatttacct cggtttcggt atcggtagca
    10081 tttttggttc gattatcgcc tcactgtttg gcggtttcta tgtgaccttc tacgtgattt
    10141 tcgccctgct gattctgtca ttggcgcttt ctacgacgat tcgtcagcca gagcagaaaa
    10201 tgttgcgtga ggcgcatggc tccctttaat gctcagccgc gagctactgg gcttcagctt
    10261 ccgtgtgcat caaacgcatc aacgaaatgt ataaacaatg actcgtaaaa tttgggccag
    10321 ttaactggcc ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt
    10381 tgtaaatttg tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac
    10441 ttattcgcgc gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc
    10501 tgaaaagccg ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata
    10561 catcaaatcg attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt
    10621 tatgaatggc gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg
    10681 gaaaggcgtt tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact
    10741 acgtcttcta tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct
    10801 cttttccgca ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat
    10861 catcgcttcc gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt
    10921 tattctctgg ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg
    10981 tcgctacacc ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggtggt
    11041 cgtcgtactg gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa
    11101 gaaagtggag agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc
    11161 ttatctgcca tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga
    11221 atcttccgat aataattcat tgcttaatcc ggcgaagaag tttacatacc aggctccaca
    11281 aaacgttgat gatacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat
    11341 gggtattttc ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc
    11401 ggcgttccgt ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt
    11461 acgtcagggg ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc
    11521 ggttctgaag cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt
    11581 ctacagcaac acgatggcag acaacattgc ttatcgtgag cagattggtg cggagccacg
    11641 taatcgtggc aagccggtag atgatatgtt gctggtagac gaaatgcagc aatcgctagg
    11701 gcgcaacccg gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta
    11761 cacccagcgt tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag
    11821 cggctgtacc aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca
    11881 ctttatctcc agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc
    11941 tgaccacggt gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc
    12001 accgccggag cagttccgcg taccgatgat ggtctggatg tcagataaat atctggaaaa
    12061 tccggccaat gcgcaggcgt ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg
    12121 ccgtcacgta gagctgtacg acaccatcat gggttgtctt ggctatactt caccggatgg
    12181 tggaattaac gaaaacaaca actggtgtca catcccgcag acaaaagtgg cagcggctaa
    12241 ctaagcggcc tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg
    12301 agggcgtatc tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg
    12361 cacttcgttc gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa
    12421 aagcctgctc aacgagcagg tttttttgca tttgtagctc atgaggatga gaacctccgg
    12481 gggcagggag gctcgactcg acgtaggcct gataagacgc gccagcgtcg catcaggcaa
    12541 cggcaccgca cttattgtcg gatgcggcgc gaccgcctta ttcgaccaac acaccacaca
    12601 ttttcctgcc aactttgcgc cccatatctc actatccatt aacggatttg tgacagaaac
    12661 cattgtcttt ttttatattt gcttaaatct tttttgcgtt acttatggct ggcgctggca
    12721 ttttccgctg tgcgttttag cgttcatggt attaatcgct cagatatcca ccttttcctc
    12781 agattttttt cattaaatgc ataaaactta atcaccaaat gttgctaaat attaagctga
    12841 ttgttctggt tatgttggag tagcacaaat ttctctgctg caaatagctg tttgaagttt
    12901 ttttaatctt tgtttgtgga ttctcaccgt tggtgtaaat cccggttggc tgtattgacg
    12961 ttttcacatt ctgttgacag attgtaggtc acgaggggca ttttatggag gatccgcact
    13021 gttacactga tgttaattag tacggcatcc ccacctcata acgttgaccc gaccgggcaa
    13081 aaaacaaaaa aggtcaggca gcgacaaccc actgcaaagg gttaaaacaa caaacatcac
    13141 aattggagca gaataatgcg tatttccttg aaaaagtcag ggatgctgaa gcttggtctc
    13201 agcctggtgg ctatgaccgt cgcagcaagt gttcaggcta aaactctggt ttattgctca
    13261 gaaggatctc cggaagggtt taacccgcag ctgtttacct ccggcaccac ctatgacgcc
    13321 tcttccgtcc cgctttataa ccgcctggtt gaatttaaaa tcggcaccac cgaagtgatc
    13381 ccgggcctcg ctgaaaagtg ggaagtcagc gaagacggta aaacctatac cttccatctg
    13441 cgtaaaggtg tgaagtggca cgacaataaa gaattcaaac cgacgcgcga aatgaacgcc
    13501 gatgacgtgg tgttctcgtt cgatcgtcag aaaaacgcgc aaaacccgta ccataaagtt
    13561 tctggcggca gctacgaata cttcgaaggt atgggcttgc cggagctgat cagcgaagtg
    13621 aaaaaggtgg acgataacac cgttcagttt gtgctgactc gaccggaagc gccgttcctc
    13681 gctgacctgg caatggactt cgcctctatt ctgtcaaaag aatatgctga cgcgatgatg
    13741 aaagccggta caccggaaaa actggacctc aacccaatcg gaaccggtcc gttccagtta
    13801 cagcagtacc aaaaagattc ccgtatccgc tacaaagcgt ttgatggcta ctggggcacc
    13861 aaaccgcaga tcgatacgct ggtcttctct attacccctg atgcttccgt gcgttacgcg
    13921 aaattgcaga agaatgaatg ccaggtgatg ccgtacccga acccggcaga catcgctcgc
    13981 atgaagcagg ataaatccat caacctgatg gaaatgccgg ggctgaacgt cggctacctc
    14041 tcgtataacg tgcagaaaaa accgctggat gacgtgaaag ttcgccaagc actaacctac
    14101 gcggtgaaca aagacgcgat catcaaagcg gtttatcagg gcgcgggtgt atcagcgaaa
    14161 aacctgatcc cgccaaccat gtggggctat aacgacgacg ttcaggatta cacctacgat
    14221 cctgaaaaag cgaaagcctt gctgaaagaa gcgggtctgg aaaaaggttt ctccatcgac
    14281 ctgtgggcga tgccggtaca acgtccgtat aacccgaacg ctcgccgcat ggcggagatg
    14341 attcaggcag actgggcgaa agtgggcgtg caggccaaaa tcgtcaccta cgaatggggt
    14401 gagtacctca agcgtgcgaa agatggcgaa catcagacgg taatgatggg ctggactggc
    14461 gataacgggg acccggataa cttcttcgcc accctgttca gctgcgccgc ctctgaacaa
    14521 ggttctaact actcaaaatg gtgctacaaa ccgtttgaag atctgattca accggcgcgt
    14581 gctaccgacg accataacaa acgcgttgaa ctgtacaagc aagcgcaggt ggtgatgcac
    14641 gatcaggctc cggcactgat catcgctcac tccaccgtgt ttgaaccggt acgcaaagaa
    14701 gtcaaaggct atgtggttga tccattaggc aaacatcact tcgaaaacgt ctctatcgaa
    14761 taattaaaag ccatacaaga ctgatggcaa aggcaaaact gcctgatgtg ctccgcttat
    14821 caggcctacg aaaattctgc aatgtattga atttgcacga ttttgtaggc cggataaggc
    14881 gttaacgccg catccggcat aaacaaagcg cactttgtca acaatctgta tacccggtgg
    14941 cgctgtgcct ctgcgtgcag cgccatccgg cagcaatact tcattctctg tccgacaagg
    15001 gcgggaattg atttgtgagc aatacagaca cgcagttcca ggctgcgagt cactatagag
    15061 aatccgggtt atgttgcagt ttattctccg acgtttggga ctcgtcatcc ccacgtttat
    15121 cggtattacc cttctcacat ttgcctttgt ccacatgatc ccgggcgatc cggtgatgat
    15181 catggcaggc gaacgtggga tctcccctga gcgtcacgtg cagctgctgg ctgaactcgg
    15241 cttagataaa ccgatgtggc agcagtatct ccattacatt tggggcgtaa tgcacggcga
    15301 tctaggcatt tcaatgaaaa gccgaattcc agtatgggaa gagttcgtgc cgcgctttca
    15361 ggccacgctg gaacttggcg tctgcgcgat gatttttgct acggcagtcg gtattccggt
    15421 tggtgtgctg gctgcggtta aacgcggttc cattttcgat cacactgcag ttggcctggc
    15481 gctgaccggc tactcgatgc cgatcttctg gtggggcatg atgctgatca tgctggtttc
    15541 ggtgcactgg aacctgacgc ccgtctccgg tcgcgtgagc gatatggtgt tcctcgatga
    15601 ctccaatccg ttaaccggtt ttatgctaat cgacaccgcc atctggggtg aagacggcaa
    15661 ctttatcgat gccgtcgccc atatgatctt gcccgccatt gtgctgggta ctattccgct
    15721 agcggtcatt gtgcgtatga cacgctcctc gatgctggaa gtgctgggcg aggattacat
    15781 ccgcaccgcg cgcgccaaag ggctaacccg catgcgggtg attatcgtcc atgcgctgcg
    15841 taacgcgatg ctgccggtag tgaccgttat cggcctccag gtgggaacat tgctggcggg
    15901 ggcgattctg accgaaacca tcttctcgtg gcccggtctg gggcgctggt tgattgacgc
    15961 actgcaacgc cgcgactatc cggtagtgca gggcggcgta ttgctggtgg cgacgatgat
    16021 tatcctcgtc aacttgctgg tcgatctgct gtacggcgtg gtgaacccgc gtattcgtca
    16081 taagaagtaa ggggacatca tgtcacaggt tactgaaaat aaagtgatta gcgcaccggt
    16141 gccgatgacc ccgttacagg agttctggca ctattttaaa cgcaacaaag gcgcggtcgt
    16201 cgggctggtt tacgtcgcca tcgtgctgtt catcgcgatc tttgccaact ggattgcgcc
    16261 ttataacccg gcggatcagt tccgcgatgc gctgctcgcc ccgccagcct ggcaggaagg
    16321 cggcagtatg gcacacttgc tgggcaccga tgacgtcggt cgtgatgtgc tgtcgcggct
    16381 gatgtacggc gcgcgtttat cgctgctggt tggctgtctg gtggtggtgt tgtcgctgat
    16441 tatgggcgtt attctcggcc tgatcgccgg ttacttcggc ggcctggttg ataacattat
    16501 catgcgcgtg gtcgatatca tgctggcgct gccaagtctg ctgttggcac tggtgctggt
    16561 ggcgattttc ggcccgtcga ttggtaacgc cgcgctggcg ctgaccttcg ttgccttgcc
    16621 gcactatgtg cgcttaaccc gcgccgccgt gctggtggag gtgaatcgcg attacgtcac
    16681 cgcctctcgc gtggcaggtg ccggggcgat gcgccagatg tttattaaca tcttcccgaa
    16741 ctgccttgcg ccgctgattg ttcaggcgtc gctcggtttc tctaacgcca ttctcgatat
    16801 ggctgctctt ggtttcctcg gcatgggggc acagccgcca acgcctgagt ggggcaccat
    16861 gctctccgac gtgttgcagt tcgcgcaaag cgcctggtgg gtcgtgacct tccctggtct
    16921 ggcgatcctg ttgacggtgc tggcatttaa cctgatgggt gacggtctgc gtgacgcgct
    16981 cgatcccaaa ctaaagcagt aagaggttcg agatggcgtt attaaatgta gataaattat
    17041 cggtgcattt cggcgacgaa agcgcaccgt tccgcgccgt agaccgcatc agctacagcg
    17101 taaaacaggg cgaagtggtc gggattgtgg gtgagtccgg ctccggtaag tcggtcagtt
    17161 cactggcgat tatggggctg attgattatc cgggccgcgt aatggcagaa aaactggagt
    17221 ttaacggcca ggatttgcag cgtatctcag aaaaagagcg ccgcaacctg gtgggtgccg
    17281 aagtggcgat gatcttccag gacccgatga ccagccttaa cccgtgctac accgtgggtt
    17341 tccagattat ggaagcgatt aaagtgcatc agggcggcaa caaaagtacc cgccgtcagc
    17401 gggcgattga cctgctgaat caggtcggta ttcccgatcc ggcttcgcgt ctggatgttt
    17461 acccgcatca gctttccggc ggcatgagcc agcgcgtgat gatcgccatg gcgattgcct
    17521 gtcggccaaa actgctgatt gccgatgaac cgaccaccgc gctggacgtg accattcagg
    17581 cgcaaatcat cgaactactg ctggagctac agcagaaaga gaacatggcg ctggtgttaa
    17641 ttacccatga cctggcgctg gtggcggaag ctgcgcataa aatcattgtg atgtatgcag
    17701 gtcaggtagt agaaacgggc gatgcacacg ccatcttcca tgcgccgcgt cacccgtata
    17761 ctcaggcatt gctgcgtgcg ctgccagaat ttgctcagga caaagaacgt ctggcgtcgt
    17821 tgccaggtgt tgttcccggc aagtatgacc gcccgaacgg ctgcctgctt aacccgcgct
    17881 gcccctatgc cactgacaga tgtcgcgctg aagaaccggc gctgaatatg ctcgctgatg
    17941 ggcgtcagtc caaatgccat tacccacttg atgatgccgg gaggcccaca ctatgagtac
    18001 gcaagaggcc acctcgcaac aaccgctgtt gcaggctatc gacctgaaaa aacattatcc
    18061 ggtgaagaaa ggcatgttcg tgccggaacg tctggtgaag gcgctggacg gcgtttcgtt
    18121 taaccttgaa cgtggcaaaa cgctggcagt agtgggcgaa tctggctgcg gtaaatcgac
    18181 cctcggtcgg ttgctgacga tgatcgaaac gcccaccggc ggcgagctgt attatcaggg
    18241 gcaggatctg ctcaagcacg atccgcaggc gcagaagctg cgtcggcaga aaatccagat
    18301 cgtcttccag aatccctacg gttcgctgaa tccgcgtaag aaagttgggc aaattctcga
    18361 agagccgctg cttattaaca ccagcttaag caaagaacag cgtcgggaaa aagccctgtc
    18421 gatgatggca aaagtcggcc tgaaaaccga gcactatgac cgctatccgc acatgttttc
    18481 cggcggacaa cgtcagcgta tcgctattgc ccgtggtctg atgctcgacc cggatgtggt
    18541 gattgccgat gagccggttt ctgcgctgga cgtctcagtg cgtgcgcagg tgctgaatct
    18601 gatgatggat ttgcagcagg agttggggct ttcttatgtc tttatctccc acgacctgtc
    18661 ggttgtggag cacattgctg atgaagtgat ggtgatgtac ctaggccgct gcgtggagaa
    18721 aggaacgaag gaccaaatct tcaataaccc gcgccatccg tacacccagg cgctgctctc
    18781 cgcgacgcca cgcctgaacc cggacgatcg tcgtgaacgc atcaaactca ccggtgaact
    18841 gccaagcccg ctgaatcctc cgccgggttg cgccttcaac gcccgctgtc gtcggcgctt
    18901 cggcccctgc acccagttgc agccgcagct aaaagactac ggaggtcaac tggtagcttg
    18961 ttttgctgtt gatcaggatg aaaatccgca gcgttaactc acaaaaccgg ggccatgcgc
    19021 tccggtttac tcaatcagct tcaacagtgg cgacaagcat agcaaaatgc cgtaaagcag
    19081 aatcagccag gctttaaatc cgcgaagttt ttccagttgt gcgactttat agatcaggaa
    19141 aaacggaatg aggcacgaca caatgccata taacgggctt cccaactgaa agaacaccag
    19201 cactgataca cgaaacgaaa cccaaatcgt caacgtaatg acgataaaag cgcagatcgc
    19261 cagagtcagc atgcgtgagt taattttctt ggtatcaata attcggctta acagattgag
    19321 aataatgcct ttaatagcct cgtggaaacc gagataaatg ccaaagaatg cggtcagtac
    19381 ggcaaagata ttcagcaccg tagaggtgat atgaatgata tgtccaggga tcacctgcgc
    19441 ggccagtgcc agtgccgaga tattttgttc aaaggcagaa acggcttctt cgtggctaat
    19501 tgagaaggta aacgaaaagg caaaaaacag gatcaccgcg atgagcgtga tataactaat
    19561 tcggtgggta cgcagcgcga gccgggttgc cagtacttta tctgcttccc gtttacgata
    19621 ggcaatattc attgggttaa gtacctgaat aaataccgca gaaaagaagc aaaatggaat
    19681 ggtaagcagg acatcgcgga aaaagacgga ggcttgcggg aaggcggtta tattggcgaa
    19741 attccagtgc gggatcatcg caaaaccgaa cacgacaata atccctactt tgaccaccac
    19801 cattggccca gaaatcttaa acagtaatcg ttcaccacca gacgcaatcg ctatcagtac
    19861 ggcgaaaata gcgactttat aaagtagaga ttgtgaaaga tcggcatcgg ttaaaccgaa
    19921 ggttttcagg tacgaggcgc tgtcgaaaac cacggagaga gagtagataa aaatcccgtg
    19981 gataatcatc aaaaagtaga taaccccgag gaaaattccc cagttcttcc ccaggtaatg
    20041 actgataata tcggtgtagt cattacagga atcgctttca gaaagggttt ttaaataaat
    20101 gtcctgcact acccaggtga caggataagc aatgatcgct gcggtaataa atacccaaat
    20161 tcccttcaag ccaatttgta ctggcatcag cacggttccg gcaccaatcg ccatgcctat
    20221 gcataataaa acccagccga agtcgtagcg tgtaaacggc aatttctgat tgtgtttcga
    20281 tagtgtgttg tgctgcataa agtcctgtcc agaaagaagt cggcctcgca aaaatgaata
    20341 aacccagttt ctcttgcgca acaggtggag aagtcgaata cgcagtatgt aaaaaatgag
    20401 gcaacagatt cactggggag gtcgcgagga ggcgcaattc tacaaaattg cgcatttcac
    20461 taccagtctg gagtcagccc ggatatgaac tgcaactatt gcaaataaag aaattaagct
    20521 ggaaggcatc gcgcaatgag gaagattgcc gggtgctgac agtaaaaagc a
//
LOCUS       DAJTUP010000069        20525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTUP010000069 DAJTUP010000000
VERSION     DAJTUP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.069"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            86..385
                     /locus_tag="OO468_002618"
     CDS             86..385
                     /locus_tag="OO468_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6259688.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            395..919
                     /gene="ygaP"
                     /locus_tag="OO468_002619"
     CDS             395..919
                     /gene="ygaP"
                     /locus_tag="OO468_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR6259689.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO468_002620"
     CDS             complement(966..1370)
                     /gene="stpA"
                     /locus_tag="OO468_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR6259690.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2038..2487
                     /gene="alaE"
                     /locus_tag="OO468_002621"
     CDS             2038..2487
                     /gene="alaE"
                     /locus_tag="OO468_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR6259691.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2524..2868)
                     /locus_tag="OO468_002622"
     CDS             complement(2524..2868)
                     /locus_tag="OO468_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR6259692.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3020..3250
                     /locus_tag="OO468_002623"
     CDS             3020..3250
                     /locus_tag="OO468_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259693.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3597..3842
                     /gene="nrdH"
                     /locus_tag="OO468_002624"
     CDS             3597..3842
                     /gene="nrdH"
                     /locus_tag="OO468_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR6259694.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3839..4249
                     /gene="nrdI"
                     /locus_tag="OO468_002625"
     CDS             3839..4249
                     /gene="nrdI"
                     /locus_tag="OO468_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR6259695.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4222..6366
                     /gene="nrdE"
                     /locus_tag="OO468_002626"
     CDS             4222..6366
                     /gene="nrdE"
                     /locus_tag="OO468_002626"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR6259696.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6376..7335
                     /gene="nrdF"
                     /locus_tag="OO468_002627"
     CDS             6376..7335
                     /gene="nrdF"
                     /locus_tag="OO468_002627"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR6259697.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7690..8892
                     /gene="proV"
                     /locus_tag="OO468_002628"
     CDS             7690..8892
                     /gene="proV"
                     /locus_tag="OO468_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR6259698.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8885..9949
                     /gene="proW"
                     /locus_tag="OO468_002629"
     CDS             8885..9949
                     /gene="proW"
                     /locus_tag="OO468_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR6259699.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10007..10999
                     /gene="proX"
                     /locus_tag="OO468_002630"
     CDS             10007..10999
                     /gene="proX"
                     /locus_tag="OO468_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR6259700.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11191..12370
                     /locus_tag="OO468_002631"
                     /pseudo
     CDS             11191..12370
                     /locus_tag="OO468_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO468_002632"
     CDS             12494..13231
                     /gene="ygaZ"
                     /locus_tag="OO468_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR6259701.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13221..13556
                     /gene="ygaH"
                     /locus_tag="OO468_002633"
     CDS             13221..13556
                     /gene="ygaH"
                     /locus_tag="OO468_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR6259702.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13647..14177
                     /gene="emrR"
                     /locus_tag="OO468_002634"
     CDS             13647..14177
                     /gene="emrR"
                     /locus_tag="OO468_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR6259703.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14304..15476
                     /gene="emrA"
                     /locus_tag="OO468_002635"
                     /pseudo
     CDS             14304..15476
                     /gene="emrA"
                     /locus_tag="OO468_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15493..17031
                     /gene="emrB"
                     /locus_tag="OO468_002636"
     CDS             15493..17031
                     /gene="emrB"
                     /locus_tag="OO468_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR6259704.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO468_002637"
     CDS             complement(17095..17610)
                     /gene="luxS"
                     /locus_tag="OO468_002637"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR6259705.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO468_002638"
     CDS             complement(17760..19316)
                     /gene="gshA"
                     /locus_tag="OO468_002638"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6259706.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19389..19817)
                     /locus_tag="OO468_002639"
     CDS             complement(19389..19817)
                     /locus_tag="OO468_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR6259707.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO468_002640"
     CDS             complement(19814..20380)
                     /gene="yqaB"
                     /locus_tag="OO468_002640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR6259708.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4956 a         5248 c         5125 g         5196 t
ORIGIN      
        1 tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt agagtttact
       61 taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg ccagtaccga
      121 acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga ttctgtgcat
      181 gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg gactgagcgc
      241 ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca acagccaacg
      301 ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca ttatcgccac
      361 cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca tttcgccgca
      421 tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg acgctgatga
      481 atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc tggaacagtc
      541 aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg caggtaaacg
      601 caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa tatttttact
      661 cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa ataaatctca
      721 acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat taaacggcgt
      781 tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg ttggtgccgg
      841 gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag ataagatgcc
      901 gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac atccggcctc
      961 agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg agcaattggc
     1021 ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc agtgaattta
     1081 tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc agagctatta
     1141 cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca ggtgctaatt
     1201 ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg tctttcttta
     1261 gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa ttcgcgagcc
     1321 atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat aataaagaaa
     1381 accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat cacttattat
     1441 atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc tgttattttc
     1501 tgaaactctc agaataattt caagattata tattagccat tttgatgagt aatttaggtt
     1561 attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca aaaccgaagt
     1621 gaataaaaat gcaatataac agaataaaag aaaacgcccc tcactggtgc tttctgctgt
     1681 taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac aaacaccgaa
     1741 ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat taaaggcaag
     1801 atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa tccacaatgt
     1861 acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat aaaaattttt
     1921 cactaattga ttagtcatag acagcgatat acgctatgca aaaatgcaga tggcaatgag
     1981 atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg
     2041 ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac
     2101 tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct
     2161 ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg
     2221 taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat
     2281 ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta
     2341 gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg
     2401 atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc
     2461 agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc gccagtcgcg
     2521 tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact
     2581 aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg
     2641 ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc
     2701 ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata
     2761 gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa
     2821 accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg
     2881 ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt
     2941 ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg
     3001 aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt
     3061 gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattgaagtc
     3121 ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcgttgct
     3181 gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cgcgcgatgc
     3241 cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc
     3301 tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga
     3361 taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt gcctggtgaa
     3421 tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat cactatttgc
     3481 tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga
     3541 gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc
     3601 gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg
     3661 aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcagcggaag
     3721 cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt
     3781 ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat
     3841 gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta tcgaacgttt
     3901 aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc
     3961 ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca
     4021 ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc
     4081 tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg
     4141 cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt
     4201 tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg
     4261 atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg tcgcattcag
     4321 ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt
     4381 gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat
     4441 gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac
     4501 accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg
     4561 ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg
     4621 gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga tgaaatgctg
     4681 tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc
     4741 gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat tggtcgggcg
     4801 gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt gctgtcgaat
     4861 ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtaattccg
     4921 gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc gcgtcagggg
     4981 gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct cgacacgaaa
     5041 cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg
     5101 gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccctatgac
     5161 gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca ctatgacgaa
     5221 ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt cttccagcga
     5281 ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga cacagtaaac
     5341 cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag
     5401 gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg ccatgatatt
     5461 tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga tttttcccgc
     5521 acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca tatccgcagc
     5581 gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg gcagatgaat
     5641 ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc cctggatttc
     5701 accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggcg
     5761 cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag tggtgaatat
     5821 ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg cgaactgttt
     5881 gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg
     5941 atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg gtctatctct
     6001 tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa
     6061 gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga aaatctggcg
     6121 ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta cgcggaagcg
     6181 actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac cgccaccact
     6241 cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac
     6301 atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca
     6361 ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct
     6421 gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag
     6481 gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga acaacaactg
     6541 acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg
     6601 ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc gaatatcagc
     6661 tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgccagacc
     6721 aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct
     6781 cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg
     6841 tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc
     6901 ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga agcagtccac
     6961 ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt
     7021 gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa cgagttgcaa
     7081 tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt
     7141 tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg
     7201 gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat
     7261 ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa
     7321 gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat
     7381 tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt
     7441 tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat
     7501 atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg cgatttgctc
     7561 gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg
     7621 gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat aaagaaatca
     7681 ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt tggtgagcat
     7741 ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa
     7801 actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt
     7861 gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg
     7921 attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgac
     7981 gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta
     8041 atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat
     8101 gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat
     8161 gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc
     8221 gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca
     8281 ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc
     8341 attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg aattgccatt
     8401 atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg
     8461 aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt cagtgcgaaa
     8521 gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt cggcccacgt
     8581 tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt
     8641 aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa
     8701 ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc
     8761 gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa
     8821 cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta
     8881 aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca
     8941 atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg gtgctgactg
     9001 gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa
     9061 aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt
     9121 ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca
     9181 attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca tcacctggca
     9241 gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca tcggcgcaat
     9301 cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc tgctgttctg
     9361 tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag cggcgaaaat
     9421 tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat
     9481 cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgcgct
     9541 gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga
     9601 agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct
     9661 ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt
     9721 ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg
     9781 tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat
     9841 cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg
     9901 gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg
     9961 cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt
    10021 actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc
    10081 gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa ccttccagac
    10141 gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac ccagcgaagt
    10201 ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa
    10261 ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga aattttatcg
    10321 tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga aaaccgccga
    10381 ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga
    10441 taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg
    10501 tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc ataatcaggg
    10561 gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt
    10621 ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga aagatgtggt
    10681 ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata ccaaactgcc
    10741 gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca acaaagcctg
    10801 gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc cagtggccga
    10861 tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg
    10921 ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc
    10981 gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc tcatccgccg
    11041 ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca aaccacatcg
    11101 cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt attgatttcc
    11161 agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga gcttagcccg
    11221 gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa ctattacgcc
    11281 cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc ggcaggcttt
    11341 attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt tcccctcggt
    11401 gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc gggcggtatg
    11461 ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc attaaccggt
    11521 ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc ttcaccggat
    11581 aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat attgctggca
    11641 cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt ttgggttgct
    11701 tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca aatgaaatca
    11761 gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt catcagcgat
    11821 aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt cagcattctc
    11881 tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga tggtgacatt
    11941 ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc gggcggtttt
    12001 gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac tttcatggct
    12061 ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc tggtgctgga
    12121 cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa tacatcctga
    12181 tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg gtgccgccgg
    12241 ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt gtctggctgg
    12301 cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc gtcaggaagc
    12361 cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg tgttaagaag
    12421 cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt aacaatcgtt
    12481 aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg cgaccttcat
    12541 ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg cctttgcgtt
    12601 cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt tctcctgcat
    12661 catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg ggagtagttt
    12721 gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt atggcccgtc
    12781 actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat gggcgtttgg
    12841 cctgacggat gaggtttttg ccgccgccac cgcaaaactg gtacgcaata atcgccgctg
    12901 gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg tatttggtac
    12961 ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg ttgaagctgc
    13021 attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt tccagcgcaa
    13081 acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa cgctattttc
    13141 tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt taatccaggc
    13201 attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta ctggttggcg
    13261 cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat gcccgcccaa
    13321 ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg atatgcgctc
    13381 tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc gtgcccacgc
    13441 tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt atcatcccaa
    13501 cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt atataaccca
    13561 caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt tactatatcg
    13621 gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt gaacaaatgc
    13681 taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt ctgactcgtc
    13741 tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg aaggctcagg
    13801 ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa aaccacagta
    13861 ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg acgcgtattg
    13921 ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac gatcgccgct
    13981 gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt ttaccaccgc
    14041 agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa gatcagctcg
    14101 agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac ggtgtggttc
    14161 tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa aagaaaaaat
    14221 gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc agccgatgag
    14281 ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc ccgcagcaac
    14341 cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg ctctttataa
    14401 ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc gaagaaaccg
    14461 atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct ggcagcgtga
    14521 cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg gtcactctcg
    14581 acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct tccagcgttc
    14641 gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt gaggtgcaaa
    14701 aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg ggcaatgcca
    14761 acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt gcccaggcgc
    14821 aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg gggactaaac
    14881 tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc tagctggcgc
    14941 tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt gccgtgcaac
    15001 cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc accaatatgt
    15061 gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt cagccggtca
    15121 ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg gttggtctgg
    15181 atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc ggtaactgga
    15241 tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag ctggagcaat
    15301 acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac cgtgacggtc
    15361 aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg cgtgaaatca
    15421 gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct ggctaatcca
    15481 gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt cattatgacg
    15541 attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc taacgtggcg
    15601 atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg ggtaatcact
    15661 tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc aaagcgcgtc
    15721 ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc ttgggcgtgt
    15781 ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg gattgtcgcc
    15841 gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc agccaaacgc
    15901 tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg cggcccgatc
    15961 ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat caacgtgccg
    16021 attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga aacccgcacc
    16081 gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat cggcagcctg
    16141 cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga aattatcatt
    16201 cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct gaccgacgat
    16261 aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg ctgcttgtgt
    16321 atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca gttgttgcag
    16381 gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt agggattatt
    16441 ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat gcggcggctg
    16501 gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta tacctttgaa
    16561 ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt tgcggtggtc
    16621 tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga acgactggcg
    16681 gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg cacgtcgata
    16741 accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac tgagtcggta
    16801 aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct tgggatgacg
    16861 caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct gattatttcc
    16921 gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg gctggtgtgg
    16981 tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta agtacaacta
    17041 agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta ctgactagat
    17101 gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt tgatgcgtac
    17161 ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt gcatctggta
    17221 agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca ctttcagcac
    17281 gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg taccaatcag
    17341 actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct ctacaccatt
    17401 accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat ggatccctct
    17461 ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg tgattgcgtc
    17521 gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca tccgggtatg
    17581 atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt ttttaaaaat
    17641 tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca tttgttatca
    17701 tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt tcttttctgt
    17761 caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc catttcctgc
    17821 tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag aatttccagc
    17881 ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt tccgccaata
    17941 ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg attatcgaag
    18001 caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat gctatccagc
    18061 gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag taacgggaac
    18121 tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc aaggatcacc
    18181 cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg tgcatcagcc
    18241 agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc tacaccaatc
    18301 ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc gccacgtaac
    18361 agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat cggcgcatac
    18421 agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc gtctttctca
    18481 ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag gcccgctacg
    18541 tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt ggtatagccg
    18601 agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca ctcggttttc
    18661 tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc cggagatgca
    18721 ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac gcggaaatag
    18781 cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt cgcttgccag
    18841 aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat cagcgcgccg
    18901 tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt agaagtgccg
    18961 tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact taacggccac
    19021 atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat aaaggtcagc
    19081 atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc cgcaaaatcg
    19141 gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc tgttgttgcc
    19201 agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg tatccccttt
    19261 aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg gatcaaattg
    19321 acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc atatgtgcag
    19381 gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac tgcaacatag
    19441 cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat gcgcatccat
    19501 cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa tagcgtgact
    19561 gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt tttacgcaat
    19621 ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc cattgttgct
    19681 gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac cagcacgact
    19741 tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa cgagaaaagc
    19801 gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct gcacgggccg
    19861 cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc acgcccatac
    19921 gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg tgatcagcag
    19981 cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc tcagcgatgg
    20041 cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt ttcaccactt
    20101 caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct tctgtttttt
    20161 cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt gcctgagcaa
    20221 tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac tgaagaccgt
    20281 agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc aggattgtgc
    20341 catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg caagtcgaaa
    20401 aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa ggagaagaaa
    20461 attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg aaattttttg
    20521 gagaa
//
LOCUS       DAJTUP010000070        20399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTUP010000070 DAJTUP010000000
VERSION     DAJTUP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.070"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO468_002641"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO468_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR6259709.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO468_002642"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO468_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR6259710.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO468_002643"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO468_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR6259711.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO468_002644"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO468_002644"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR6259712.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OO468_002645"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OO468_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR6259713.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OO468_002646"
     CDS             complement(8214..9107)
                     /locus_tag="OO468_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR6259714.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO468_002647"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO468_002647"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR6259715.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO468_002648"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO468_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR6259716.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OO468_002649"
     CDS             complement(10238..11470)
                     /locus_tag="OO468_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR6259717.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO468_002650"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO468_002650"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR6259718.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO468_002651"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO468_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR6259719.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16534)
                     /locus_tag="OO468_002652"
                     /pseudo
     CDS             complement(14275..16534)
                     /locus_tag="OO468_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16741..17025)
                     /gene="ihfB"
                     /locus_tag="OO468_002653"
     CDS             complement(16741..17025)
                     /gene="ihfB"
                     /locus_tag="OO468_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR6259720.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17185..18858)
                     /gene="rpsA"
                     /locus_tag="OO468_002654"
     CDS             complement(17185..18858)
                     /gene="rpsA"
                     /locus_tag="OO468_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR6259721.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18969..19652)
                     /gene="cmk"
                     /locus_tag="OO468_002655"
     CDS             complement(18969..19652)
                     /gene="cmk"
                     /locus_tag="OO468_002655"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR6259722.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19825..>20399)
                     /gene="ycaL"
                     /locus_tag="OO468_002656"
     CDS             complement(19825..>20399)
                     /gene="ycaL"
                     /locus_tag="OO468_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR6259723.1"
                     /translation="QVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAM
                     ANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSG
                     VASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASL
                     DGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4891 a         5656 c         4970 g         4882 t
ORIGIN      
        1 tatgtgccat ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactcg cctgggaagg cgcttcgtct cttcctagaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaaca tgttcggtat tctggaagac
      181 tttacgtacc agtttatagg tggtgccttt ttccggactg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccaaccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccaa
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttga gacgcacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ctcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcacgg gaggttaatt cttcggtcag acggctaagt tcaatctcca tctgttcgat
      901 agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ctccggacgt ttcggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ctaacgcctt
     1081 atccgacatg gaacgcaaat catcagcgga gagataagcc agctcacgac ggtgtaagcg
     1141 gcgctcaacg ccgttatctt tcaccatgcg catcaccgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 gttgtccatc tccgcttcgc agaaggtaag cgctttttcc agttgattgc ggcgtgaacg
     1321 gttattgctc agttgcgcat gcagctcgtc acggcgaata cgcgcccgct cttctgcccc
     1381 gctatcagca cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgaccg cgcaacgctt cgcgagcgcg ggtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagt ttttcgttga gatcgctgtt accgctaagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcttgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttaac tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgactc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgtc gatcggcgat tttcaccacc accgcttttt ccagctcatc
     2281 aacgctgaac acgctatcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc ggttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagagccga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacaccacc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacgg cgtttttgcg
     2581 cgcgcctact tcatcgcgtt caacaatcgc ctcgcgctca cgctccagca actgttgcag
     2641 atattcagtg acgtcctggc tggaggagaa ctcttcgccg cactgttcac tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg tgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct gccgcagtgc catccgctct tcacgggcgt tagatacgct
     2821 atcggaaaga gaggcaatgc gtgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa atctgccagc agacgctcag cttcttgctg
     2941 ctcacgcaga cgctgttcca gttcgcttaa gcgcatccgc aacggctgaa cctgctctgc
     3001 caggtgacgc tgatcaaccc cttcgcgcaa tagttcgcga gcgacatccc acgcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcac gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc attctcttcc tggcgttcga tggcttctgc
     3421 caccacttca ttttgctctt ccagacggat ctgcagctca tcgagatcca cttcgtagcg
     3481 ttcgatcttc tcctgctgac gcaatgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat caccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta gctcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatcgtggt ggatttaccc gccccgttac cgccggaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ctctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcggaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gtttcgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccggcat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc tagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtact tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cactccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaataacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccgccgcca gcatccagca cacgcagttt ttgcgggccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctagcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgggaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgag ggtatccgtt
     7801 tatggcgcga aaatccgggt tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgta ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaaggg ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcacgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat catcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cctttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagcgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atcctcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgtggt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcgacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccagag ccgaggtagc
    12721 ttcatccaga atcagaatcg ggctatcacg cagcaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcca tggcgtaggc catccgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcaaccgtct tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg tacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgatcag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 actggtggtc acttgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatactacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg atcgacgcac cttcacgcac
    13801 aacagtaatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaagaatgaa actggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagt ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgctttg aaaggcgcaa tggttggcca cagtcggcgg aatgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctctctgag
    14341 gctgctaatc cgccagcctt gtgctgaaaa attgaccgtc acttgcccct gatgtggagt
    14401 atcaagccat tgatatcctt tctgttgata gcgatgctta actttgttag agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgcctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagctggg gcttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcccttatc
    14701 gttgctacct tgtaaaggcc agtgcgcgct gaaacgcaat ccttgccact gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatatatgc aatattgaat ccagccctcc ccggtgatcc agatgttcat ggctcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttca ggccagtcct gtgtcataga gaatcgcttt gccgtttctg gctatcacca
    15001 ttgccagccc ttgcccgaca tcaagcatgt acagctgcca ctcgtcaggt cgaggttttt
    15061 gccacagcgg ccagcacatc agcaagcctc cagccacaca cattgctggc aacgttcgcc
    15121 aggcgtttaa tcgccatacc accagtaaga accatgggga aaatgatagc cattgccaac
    15181 attcaacaat gttgatccac ccttccggca aactctttaa cccccagaaa agtaaagcca
    15241 aagaccggtc ggcaagaaac cataacccct gttccaggat taacggcccg ctaaaatgca
    15301 caaccatcgc ggcgaggatc aacggaaccg tgataaatgt caccaaggga attgcaaata
    15361 gatttgcaat aaacgaggtc agactaatgc catgaaatat gacgatttgc atgggcataa
    15421 gcaggagtgt gattcccagt tgcagatgga tgagggaaac aactgcacgc aataccggcg
    15481 gcagttgcca ctcaggacag ggaaaccact gataccaaaa aatcagtgcc gcgaccgcag
    15541 cggcagagag ccataaactt tgcgagagaa tggcaacagg atccatcagc aaaattgccg
    15601 ccagacaaca tatccataca tcccagccac tccactgtcg cccacttaac ttaagcattc
    15661 cccacgtagc aagcgccacc acggtacgca atgcaggagg ttgcattccc gtcaaccagg
    15721 cataaaaagc agcacagcag attccgccaa ttaatggtat ttgccagtgg atccagcgcc
    15781 caggcagaaa aatttgtcca ctgcgaatga gtccggcagc cagcaacgcc gcaaaagcga
    15841 tgtgcaatcc cgatatcgcc attaaatgcg ccgttccagt atcgcgcatg atatttttga
    15901 tttctttggg gacggataac cgttccccca tacctaaacc aagaataacc gcattccacg
    15961 gatagggttg cagcgttgtt tgtagtgacg ccagatactg tgcacgcagg ctacaattcg
    16021 gttcaatgac acttgcctgc agaaaacggc cggtgagcgg ctgatgctgg gcaatggcat
    16081 aacgctggct atcaaagccg ccatcattaa gttggccatg aactgcacga actttgagtt
    16141 tcattgacca ttgttgtcca gcacaaaccg cttgcggaag ttattcgcca tacagcacaa
    16201 ggcctggcgc agggaatata cgtcgacctt gtagataagt aatttgaccg taatgagtgg
    16261 tcatgccgtc agttgcagtg atctcaacaa ttgcatcctg cgtcgcgcca gttaaggttt
    16321 ctcctgccca caaaatttgc tttgctgata atatgcccca aacgaaaaac agcaacgtca
    16381 gcgcgatata acggacggtt ttaacaggga taaatgccag tacgcaggcg aagagagtca
    16441 gaaacgcaag ggttaatgtc ctaggcaatt ggggcaaaat cagcaacgga aaaattccgc
    16501 aaattatgca tacgccgact gtcgttattt tcatgcaccc ctccctgttt atggaaagtg
    16561 tgcaactttg ttgagcggtt gtctggtggg aattttgcgt tccccgaggc acattcagaa
    16621 gaatttaacg aaagggtgaa aactgctcaa aactctgcga ggcagcttcc agttcctgat
    16681 tagagaattg aaaaaagcac ccgacaggtg cttttctcgc gttcaagttt gagtaaaaac
    16741 ttaaccgtaa atattggcgc gatcgcgcag ttctttacca ggtttaaagt gaggaacgta
    16801 ttttccttcc agttctactt tatcgccagt cttcggatta cgtccggtac gtggtgcgcg
    16861 gtagtgcaaa gagaaactgc cgaaaccgcg gatttcaata cgctcgccct gcgcaagagt
    16921 cgaggccata tgctccagca tctcttttac tgcatcttca accgtcttgg cgggaatgtg
    16981 cgattgctgg gtggcaagtc tttctatcaa ttctgacttg gtcatgattc ctccggttcc
    17041 ttaaaggcaa attaagcggc tgctgttgct gcattgatta agggcggccg tagccgccct
    17101 tagtgcttga ttacaggacg aaacctgcaa tctgtcaagt aaactcaaca aacttcggaa
    17161 taaaaatccc gaagagtcag agaattactc gcctttagct gctttgaaag cttcagccat
    17221 tgcgttgttg gagaagtttg catcttcctg tttgttaaca gttgcgattg catctttctc
    17281 gtcagcttcg tctttcgcac gaacagacag gctgattgcg cggtttttac gatcaacgcc
    17341 ggtgaattta gcttcaactt cgtcgccaac gctcagaacc agggtagcgt cttcaacgcg
    17401 gtcacgggat gcttcagaag cacgcaggta accttcaacg ccgtcagcca gttctacggt
    17461 tgcgccttta gcgtcaactg cagttacttt accggttacg atagcgcctt tcttgttcag
    17521 agcaacccag ttgttgaacg gatcttctgc gagctgttta acgcccaggg agatacgttc
    17581 acgttctgcg tcaacctgca gaacaactgc agcgatttcg tcgccttttt tgtattcacg
    17641 aactgcttct tcgcctgcaa cgttccagga gatgtcagac aggtgaacca ggccgtcgat
    17701 gccgccgtcc aggccgatga agataccgaa gtcagtgata gacttgattt taccttcaac
    17761 acggtcgccc ttgttgtggg tttccgcgaa ctgctgccac gggttagctt tgcactgttt
    17821 cagacccagg gagatacgac gacgttcttc gtcgatatcc agaaccataa cttccactac
    17881 atcgccaacg ttaacaactt tggacgggtg gatgtttttg ttggtccaat ccatttcgga
    17941 aacgtgtacc agaccttcaa cgccttcttc gatttcaacg aagcagccgt agtcagtcag
    18001 gttggtcacg cgaccagtca gtttggtacc ttccggataa cgtttagcga tagctaccca
    18061 cggatcttcg cccagctgtt tcaggcccag ggatacacgg gtacgttcgc ggtcgaactt
    18121 cagcacttta acagtgattt cgtcgcccac gttgacgatt tcgctcggat gcttaacgcg
    18181 tttccaggcc atgtcagtga tgtgcagcag gccgtcaacg ccgcccagat caacgaatgc
    18241 accgtagtca gtgaggttct taacgatacc tttaacttcc atgccttcct gcaggttttc
    18301 cagcagctga tcgcgctctg cgctgttttc ggattcgata acggcacgac gagaaacaac
    18361 aacgttgttg cgcttctgat ccagcttgat tactttaaat tcaagctctt tgccttccag
    18421 gtgcagagtg tcacgcaccg gacgaacgtc taccagagaa cctggcagga acgcacgaat
    18481 accgttcagc tcaacagtga agccgccctt aactttgccg ttgataacac cggtaacagt
    18541 ttcagcatct tcgtaagctt tttccagcgt gatccaggct tcgtgacgtt tagctttctc
    18601 acgggacagc agagtttcac cgaagccgtc ttctactgcg tccagagcaa cgtcaacttc
    18661 gtcacctacc tggatttcca gctcgccctg ggcgtttttg aactgctcag ccgggatggc
    18721 ggactcagat ttcagaccag cgtcaaccag tactacgtct ttgtcgatag caacaacaac
    18781 gccacgaacg atagaacccg ggcgggtttc gatttctttt aaggactctt caaagagttg
    18841 agcaaaagat tcagtcatgt ttaatcttca ggtttatatt taacgtccac ctggctccat
    18901 gccggatggg gttgtttaac atacccgctg tcattccatt gcaacggggg tactgcaaat
    18961 tcggtcgctt atgcgagagc caatttctgg cgcgcgtatt gtagcgcttt ttcaatcaat
    19021 tgctcaatgc ttaaggtggt ggaatccaac actaaagcat cggctgccgg aaccagtggc
    19081 gctaccgctc ggttacgatc gcggtcgtcg cgttctttga tctcggccaa aaggcgctcg
    19141 aagttaacac taaagccctt ctcctgcaac tgtagcatgc ggcgatgcgc acgttcttcc
    19201 gaggaggcgt caaggaaaat tttcactggt gcatcaggga ataccaccgt tcccatgtcg
    19261 cggccatcgg caatcagacc tggtaattcg cgaaacgcgc gttggcgacg caataatgct
    19321 tcacgaacgc gtgggaatgc cgcgacttgt gaagccgcat tcgccacttc ctgagtacga
    19381 atttcgccgc tgacatcttc cccttcgagg atcacttcca gattgccatt ggtcgacaca
    19441 aaacgtacat ccagatggga tgccagcggt accagcgcat cttccgacgc aacatcaaca
    19501 tggtgatgta atgccgccaa tgccagtacg cgataaattg cacccgagtc cagcagatgc
    19561 cattgcaacg cttccgccat agccttacac aaggtgcctt tccctgcacc gcttgggcca
    19621 tcaatggtaa taaccggggc aattgccgtc atctttatct ccttaaaaca ggcgtaccgt
    19681 taacataacc gcgcgcatta tacgcgccga cgccacaaat cgttaacgtt gtgtgcaaaa
    19741 accagttttg tctgtattca gatggggatt tattgcgcaa ttatagcggg ctggcagaag
    19801 accagcccga aagatgacaa tgatttactt accagaggcg atacgatcac ggatgtgttg
    19861 cgcacgctct gttgatggtg ggtgagagtc aaacatggac tgggtgcgac cgccatccag
    19921 gctagccagt ttttcaaagc tgccaaccag cccctgggtg ctgatgccac gtttcttcaa
    19981 cagatcaaaa gagaaatcat ctgcttcgga ctctttatca cgggagtatt tagcattaat
    20041 agcgccttct gcgatatcac ccaattgtga gcgggaaagc tgggaagcca caccgctggt
    20101 agctgaaatg gcatcgcgtg cggcaacgat cgcataagaa gctttcattt cagccagcga
    20161 gtgacccaac gcgacgtggc ccagttcatg gcccagaaca ccttcaattt cgttgtcatt
    20221 catcatgtcc atcaggccac tgtagacacg aacacagccg ttcgccatcg cccatgcgtt
    20281 gacgtcgctg gtcatataaa ccttatagtt gaccggcgtg ccattaatat tgttacccaa
    20341 tgctttggcg atttttgcca gacgtttacc gtacttgctg gatttacttg cgacttgat
//
LOCUS       DAJTUP010000071        20374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTUP010000071 DAJTUP010000000
VERSION     DAJTUP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.071"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..569
                     /locus_tag="OO468_002657"
     CDS             <1..569
                     /locus_tag="OO468_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6259724.1"
                     /translation="NAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAMASQR
                     AKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKMLFSHA
                     QKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVSLPDF
                     SHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            587..1225
                     /locus_tag="OO468_002658"
     CDS             587..1225
                     /locus_tag="OO468_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6259725.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1516..2244
                     /gene="mpaA"
                     /locus_tag="OO468_002659"
     CDS             1516..2244
                     /gene="mpaA"
                     /locus_tag="OO468_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR6259726.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2219..3184)
                     /gene="ycjG"
                     /locus_tag="OO468_002660"
     CDS             complement(2219..3184)
                     /gene="ycjG"
                     /locus_tag="OO468_002660"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR6259727.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3303..3809
                     /gene="tpx"
                     /locus_tag="OO468_002661"
     CDS             3303..3809
                     /gene="tpx"
                     /locus_tag="OO468_002661"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR6259728.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3853..5394)
                     /gene="tyrR"
                     /locus_tag="OO468_002662"
     CDS             complement(3853..5394)
                     /gene="tyrR"
                     /locus_tag="OO468_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR6259729.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5542..6603)
                     /locus_tag="OO468_002663"
     CDS             complement(5542..6603)
                     /locus_tag="OO468_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR6259730.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6600..7997)
                     /locus_tag="OO468_002664"
     CDS             complement(6600..7997)
                     /locus_tag="OO468_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR6259731.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8153..9151
                     /locus_tag="OO468_002665"
     CDS             8153..9151
                     /locus_tag="OO468_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6259732.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9262..10134)
                     /gene="ompG"
                     /locus_tag="OO468_002666"
                     /pseudo
     CDS             complement(9262..10134)
                     /gene="ompG"
                     /locus_tag="OO468_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10207..11288)
                     /locus_tag="OO468_002667"
                     /pseudo
     CDS             complement(10207..11288)
                     /locus_tag="OO468_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11302..11961)
                     /gene="pgmB"
                     /locus_tag="OO468_002668"
     CDS             complement(11302..11961)
                     /gene="pgmB"
                     /locus_tag="OO468_002668"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR6259733.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11958..14225)
                     /gene="ycjT"
                     /locus_tag="OO468_002669"
     CDS             complement(11958..14225)
                     /gene="ycjT"
                     /locus_tag="OO468_002669"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR6259734.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14222..14845)
                     /locus_tag="OO468_002670"
     CDS             complement(14222..14845)
                     /locus_tag="OO468_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6259735.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14842..15264)
                     /locus_tag="OO468_002671"
     CDS             complement(14842..15264)
                     /locus_tag="OO468_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR6259736.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15286..16061)
                     /locus_tag="OO468_002672"
                     /pseudo
     CDS             complement(15286..16061)
                     /locus_tag="OO468_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16079..17131)
                     /locus_tag="OO468_002673"
     CDS             complement(16079..17131)
                     /locus_tag="OO468_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR6259737.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17162..18004)
                     /locus_tag="OO468_002674"
     CDS             complement(17162..18004)
                     /locus_tag="OO468_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR6259738.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(17991..18872)
                     /locus_tag="OO468_002675"
     CDS             complement(17991..18872)
                     /locus_tag="OO468_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR6259739.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18893..20176)
                     /locus_tag="OO468_002676"
                     /pseudo
     CDS             complement(18893..20176)
                     /locus_tag="OO468_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20190..>20374)
                     /locus_tag="OO468_002677"
     CDS             complement(20190..>20374)
                     /locus_tag="OO468_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCR6259740.1"
                     /translation="SVMRIQRSNADGNCLTGLFNVSKNIQHVNITDLHGRDLISEVDI
                     LGNEITLRPWQVMWIK"
BASE COUNT         4964 a         5578 c         4967 g         4865 t
ORIGIN      
        1 ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg ggtgttaata
       61 tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta ttgaatgcaa
      121 tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac gaattacttc
      181 ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt tatgaagcaa
      241 ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa atgttgttct
      301 cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg ttactgaccc
      361 agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat cgtgatggac
      421 tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg ttacccgatt
      481 tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg gcaaaagtta
      541 ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga aaaatgtact
      601 gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg cagataaaca
      661 aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac cctatccaac
      721 taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc aggccatgca
      781 gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc aggcaaattc
      841 agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc tttccctcgg
      901 tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta ttggtgaacc
      961 cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg aatatacaat
     1021 tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta cctctcgcaa
     1081 tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa ttacagatat
     1141 catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac ccgggactga
     1201 tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac tgtactatgt
     1261 gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga ttaacatgtg
     1321 tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc taatatctgc
     1381 acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg atgacatagc
     1441 gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt aatacttcct
     1501 gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc atttccgccc
     1561 ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt tcctgcccct
     1621 gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga aaactcttcg
     1681 gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg ccatcatgtg
     1741 gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc taatggtgtg
     1801 gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt ttatcgctgg
     1861 aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc tggctccgaa
     1921 cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg ggtggtctca
     1981 ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg cgaatggctg
     2041 gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc tggctctttc
     2101 ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc gcccatctcc
     2161 tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg ctggcatcct
     2221 aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc gccagccagg
     2281 tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg gcgctaatgg
     2341 cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca cgcgcttcag
     2401 tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg ttaaccatct
     2461 catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca tcagcacaaa
     2521 tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga agcggttgtt
     2581 caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac ccttctgcac
     2641 gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg cgaattgcca
     2701 ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc gcgcctgcct
     2761 gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca accgtctgtg
     2821 cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat tgctgctgtt
     2881 ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg ccggcaggga
     2941 gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg acgctcataa
     3001 tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg cattcgccgg
     3061 tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca ctgcgacttc
     3121 cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc ttaacgattc
     3181 tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag cgtttgctgt
     3241 gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca ggaaagataa
     3301 tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat tccatcccgc
     3361 aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct gacgtcaccc
     3421 tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt gataccggtg
     3481 tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac aacaccgttg
     3541 tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc gcagaaggtc
     3601 tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa gcctacggtg
     3661 tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg gttattgacg
     3721 aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag ccggattacg
     3781 aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt gcaccatcag
     3841 gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc gcaacttatt
     3901 ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc tgggataatt
     3961 gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt ccagcgaacc
     4021 ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca acaaaatatc
     4081 ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag cgttctttaa
     4141 ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt cagcggccag
     4201 tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct cagttaacgg
     4261 cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca cgttcagacg
     4321 ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat tcttctgcgt
     4381 agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc gacggaaagt
     4441 gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt cccctatttc
     4501 atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc cttccggagc
     4561 atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat tcagcgccag
     4621 gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa gatctttacc
     4681 tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt tctgcgcctg
     4741 ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg cgctgacgtc
     4801 ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca acatcaccac
     4861 cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg taatctccat
     4921 caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg gttcgctttc
     4981 cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt tgcgcaggcg
     5041 atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca ctttgctttt
     5101 catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca acgccagatg
     5161 ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg caatacggcg
     5221 tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat tgaggtagat
     5281 tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca cgagtagatc
     5341 gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac gcatgggaac
     5401 cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat agcaggaagg
     5461 tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg taaagatatc
     5521 cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt gcagagtttc
     5581 tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt catcgtcaat
     5641 ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg tcagaagtcc
     5701 agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt gcgacatcca
     5761 gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat tcagcaatac
     5821 cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca gcgtggcaat
     5881 gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca acgccagcgg
     5941 gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc gccgcgcctg
     6001 ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac gatcgttctg
     6061 cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat caatacccgc
     6121 ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc tatgcaataa
     6181 atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc gccgccactc
     6241 tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac atccacccag
     6301 cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct gcccgacaac
     6361 gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc tgcgtttcgg
     6421 acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg cttcgtcgag
     6481 cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg ccctgaattt
     6541 tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta acggttcggt
     6601 catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat atgcggcagc
     6661 ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc ttgcttgtcc
     6721 cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt gagaagcgca
     6781 ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac gtcaataatg
     6841 ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat cctttcaaac
     6901 gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat attggcgtgc
     6961 tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc gataaccggc
     7021 gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat cagctgcgtc
     7081 agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg caggcaatcc
     7141 accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc cttcaccact
     7201 ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg cttatcggcc
     7261 tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa ttgcagcgcg
     7321 ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa gtgcagcccc
     7381 tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc cagacggttt
     7441 tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt cgccgaccat
     7501 tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa gtaatcctgc
     7561 gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc cagatacagc
     7621 gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg taacgccagg
     7681 cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc atataactgc
     7741 gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg aatgcgtttt
     7801 acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc tccggcatga
     7861 atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct gcggctaagt
     7921 ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc attgagttca
     7981 tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa aaatcattcg
     8041 taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt gtcatcgacg
     8101 ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac ctatgtcccc
     8161 tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat cacgcgtgct
     8221 gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg ccattgaaga
     8281 attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt ttgatgccat
     8341 tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt tctctgaagt
     8401 tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat tacaaacgtc
     8461 gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga aaatgattaa
     8521 aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac tcgataaata
     8581 cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg tttattctgt
     8641 cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg aaagtgggca
     8701 tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg atcgcgttaa
     8761 tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat ggattgttga
     8821 cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa gtcagtcacc
     8881 gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt atcgtgcggc
     8941 ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata gtaatgaaac
     9001 gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc aggagttagg
     9061 tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac cacagaatat
     9121 taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg aatgccggat
     9181 gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta ctcgcgccgc
     9241 atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg cataatggaa
     9301 tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca gcgaaacgga
     9361 aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat catggccttc
     9421 acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa tgcgcgtata
     9481 cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt cttgtgtgga
     9541 aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat aatagttcac
     9601 tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt cgacacgggt
     9661 atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca accaaccgtt
     9721 gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc agcgctgcat
     9781 attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac ggaaaccgcc
     9841 ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct ccagctccgg
     9901 gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt cctgataata
     9961 ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag gctccgccag
    10021 cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg caccgatatt
    10081 aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac acatcaccag
    10141 tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa tgccttcccc
    10201 ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt tcgtcatatc
    10261 aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag cacggaccac
    10321 taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa ttctgccacg
    10381 ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc ttccgggcgt
    10441 attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc taatttctct
    10501 tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat cgtgccgcga
    10561 ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc gggttggtta
    10621 taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat cacaatccgc
    10681 gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt ggtgttcagt
    10741 ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc atcaaggtta
    10801 gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg cccgagcgcc
    10861 acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta ctcacgcagg
    10921 ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc gattttttgc
    10981 atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa cgcgtagttc
    11041 tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg tttgccgtcg
    11101 atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag ggtggtcgat
    11161 ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat ttccaggttg
    11221 aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg taacgaaagc
    11281 tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc gcgaccaggt
    11341 cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac cgatccccac
    11401 cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt caatgccgat
    11461 acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt ccggatccgg
    11521 tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac gcagctccag
    11581 cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg gaatctgctg
    11641 tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa ccgtcagctc
    11701 gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc gctcctgcgg
    11761 gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact catcgcggct
    11821 gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt cagcggcaat
    11881 ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat ccagatcgaa
    11941 aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc attgaagggc
    12001 aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag ctgaccgtcc
    12061 aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc gagagtgacc
    12121 tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg ctcaagtaac
    12181 gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc ctgaatcgcc
    12241 cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact atgcggatca
    12301 gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt cagcaggcca
    12361 cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg aatagtgcgc
    12421 ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc tggcagcatg
    12481 taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac ttctgcgcgt
    12541 gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc cagattaatc
    12601 gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg aatttctggc
    12661 agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc actacagcca
    12721 aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata gctggtgaag
    12781 gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg aatttccaga
    12841 cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag aagtagcgcc
    12901 atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg aataaccgcc
    12961 caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt ttgccgcagc
    13021 ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct gcgcgcgctt
    13081 tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg cgcgcctggc
    13141 aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct aaacagatgg
    13201 aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc ttcgccggtt
    13261 aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat gcgcagatga
    13321 taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac cgtgatacgg
    13381 cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg gtcgtaactc
    13441 tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc taaagcagct
    13501 tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt ctcgcctgca
    13561 tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata gcattgctgc
    13621 acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc atcctgggtg
    13681 gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc gtcgagatgc
    13741 tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac tgacgcgtcg
    13801 gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc tgccgaaaca
    13861 aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac aacgctacgg
    13921 cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga taacgagaaa
    13981 acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac cagttcgttg
    14041 atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg cgtctggcgc
    14101 gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg cgccatcagc
    14161 gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt tactggcctg
    14221 gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc accagttgct
    14281 ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt acgtgattga
    14341 taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt tgcatcagcg
    14401 tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc gcaccggctt
    14461 tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt gcaaatgacg
    14521 tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc gaatcttcga
    14581 cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc gtgccgatct
    14641 tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca tacatcgcag
    14701 catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt tctttattgg
    14761 taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg gtaacgtaaa
    14821 tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg agcgcaaacg
    14881 atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc acatttccca
    14941 cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc ctgcttccag
    15001 tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa ccacatcagg
    15061 tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc cgtatttttc
    15121 tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt ccagatcgtt
    15181 gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc ctattatcgc
    15241 gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc aggtacgcaa
    15301 ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac gcccttcata
    15361 caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt ggaaatcgag
    15421 ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac cgagcaagtc
    15481 acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt gatagaaatc
    15541 gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag catcggcgag
    15601 ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca cggttccggt
    15661 acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc ggtcgccgtc
    15721 caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc acgctgccgg
    15781 aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa gccattaaga
    15841 cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc ggtggtcact
    15901 ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac cagcagtttg
    15961 ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct ctcgcggata
    16021 ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt cctttgaatt
    16081 aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac tgcatgtagc
    16141 tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa ttgagataac
    16201 cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag cgcggataat
    16261 ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg aaatgcgctt
    16321 cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag gagatggtgc
    16381 cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg ctggtttcga
    16441 taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct acgtcagtgc
    16501 caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag cgatgggcga
    16561 taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg atggcaattt
    16621 gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg ttggcatcgc
    16681 gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc caggagctgc
    16741 cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc gtctcggaga
    16801 gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg ctgccgcact
    16861 cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca atgccgcgcg
    16921 gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct tcatcgataa
    16981 acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg ccgaaacgca
    17041 cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc agcgcagcaa
    17101 cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga aactggccgc
    17161 tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc aaaagcgcat
    17221 acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt ccccagatgt
    17281 aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg ctggcgctgg
    17341 agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag tagacgaaca
    17401 ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt tgcagacggt
    17461 tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg aagtaacttt
    17521 tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc agcgccatct
    17581 cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc agcaaaatgc
    17641 cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct ttaaagcgca
    17701 ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg gaggaaacca
    17761 ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc gggttaaaaa
    17821 tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc agcgatatcg
    17881 cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac agcgtgatga
    17941 tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt ttatttgttg
    18001 ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat gatgaagagc
    18061 accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt tttgtagaga
    18121 tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa catgtagaac
    18181 atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc gggcagcgtg
    18241 acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc gtacagcgat
    18301 ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa gtagcgccag
    18361 atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca cagcggtgcc
    18421 tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta gccgttgttg
    18481 aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga gaggatcacc
    18541 agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc cacggccagc
    18601 ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat ccacagcgag
    18661 tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac aaacgttgac
    18721 tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga caccatcggc
    18781 cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag cgcaaacggc
    18841 atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg gtttagtgct
    18901 gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg agatcggctt
    18961 tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc acatcgccca
    19021 tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc agttcaccga
    19081 ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc caggtggcgg
    19141 tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc acccagtcgg
    19201 caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg gtcttttgtc
    19261 cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc tcggttggca
    19321 ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg atataggtgg
    19381 agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc atgatgtcgt
    19441 tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc tgcatcatct
    19501 ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag ttggataacg
    19561 caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc gcaataccgt
    19621 attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt tgccagtttt
    19681 tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg ccgccaatcc
    19741 aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca atacgcagta
    19801 cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc gctttgcgat
    19861 cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg atcacttccg
    19921 gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct tcttccacgg
    19981 gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc aatttgctga
    20041 taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat acatgggttt
    20101 tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat aacagcacga
    20161 tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg ccaggggcgc
    20221 agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc atgcagatca
    20281 gtaatattta catgctgaat atttttactg acattaaaca atccagtcag acaattacca
    20341 tcagcgttac ttctttgaat acgcattacg gatg
//
LOCUS       DAJTUP010000072        20345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTUP010000072 DAJTUP010000000
VERSION     DAJTUP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.072"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            170..511
                     /gene="raiA"
                     /locus_tag="OO468_002678"
     CDS             170..511
                     /gene="raiA"
                     /locus_tag="OO468_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR6259741.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    614..739
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            615..662
                     /gene="pheL"
                     /locus_tag="OO468_002679"
     CDS             615..662
                     /gene="pheL"
                     /locus_tag="OO468_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR6259742.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            761..1921
                     /gene="pheA"
                     /locus_tag="OO468_002680"
     CDS             761..1921
                     /gene="pheA"
                     /locus_tag="OO468_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR6259743.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1964..3085)
                     /gene="tyrA"
                     /locus_tag="OO468_002681"
     CDS             complement(1964..3085)
                     /gene="tyrA"
                     /locus_tag="OO468_002681"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR6259744.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3096..4166)
                     /gene="aroF"
                     /locus_tag="OO468_002682"
     CDS             complement(3096..4166)
                     /gene="aroF"
                     /locus_tag="OO468_002682"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR6259745.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4376..4741
                     /locus_tag="OO468_002683"
     CDS             4376..4741
                     /locus_tag="OO468_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR6259746.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4891..5409
                     /locus_tag="OO468_002684"
     CDS             4891..5409
                     /locus_tag="OO468_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR6259747.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5399..6624
                     /gene="dgcN"
                     /locus_tag="OO468_002685"
                     /pseudo
     CDS             5399..6624
                     /gene="dgcN"
                     /locus_tag="OO468_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6640..7122
                     /locus_tag="OO468_002686"
     CDS             6640..7122
                     /locus_tag="OO468_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR6259748.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7199..7546)
                     /gene="rplS"
                     /locus_tag="OO468_002687"
     CDS             complement(7199..7546)
                     /gene="rplS"
                     /locus_tag="OO468_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR6259749.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7588..8355)
                     /gene="trmD"
                     /locus_tag="OO468_002688"
     CDS             complement(7588..8355)
                     /gene="trmD"
                     /locus_tag="OO468_002688"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR6259750.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8386..8934)
                     /gene="rimM"
                     /locus_tag="OO468_002689"
     CDS             complement(8386..8934)
                     /gene="rimM"
                     /locus_tag="OO468_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR6259751.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8953..9201)
                     /gene="rpsP"
                     /locus_tag="OO468_002690"
     CDS             complement(8953..9201)
                     /gene="rpsP"
                     /locus_tag="OO468_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR6259752.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9338..10699)
                     /gene="ffh"
                     /locus_tag="OO468_002691"
     CDS             complement(9338..10699)
                     /gene="ffh"
                     /locus_tag="OO468_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR6259753.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10866..11657
                     /locus_tag="OO468_002692"
     CDS             10866..11657
                     /locus_tag="OO468_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR6259754.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11678..12964
                     /locus_tag="OO468_002693"
     CDS             11678..12964
                     /locus_tag="OO468_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR6259755.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13019..13612)
                     /gene="grpE"
                     /locus_tag="OO468_002694"
     CDS             complement(13019..13612)
                     /gene="grpE"
                     /locus_tag="OO468_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR6259756.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13735..14613
                     /gene="nadK"
                     /locus_tag="OO468_002695"
     CDS             13735..14613
                     /gene="nadK"
                     /locus_tag="OO468_002695"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR6259757.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14699..16360
                     /gene="recN"
                     /locus_tag="OO468_002696"
     CDS             14699..16360
                     /gene="recN"
                     /locus_tag="OO468_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR6259758.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16509..16850
                     /gene="bamE"
                     /locus_tag="OO468_002697"
     CDS             16509..16850
                     /gene="bamE"
                     /locus_tag="OO468_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR6259759.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16912..17202)
                     /locus_tag="OO468_002698"
     CDS             complement(16912..17202)
                     /locus_tag="OO468_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR6259760.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17192..17629)
                     /gene="ratA"
                     /locus_tag="OO468_002699"
     CDS             complement(17192..17629)
                     /gene="ratA"
                     /locus_tag="OO468_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR6259761.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17800..18282
                     /gene="smpB"
                     /locus_tag="OO468_002700"
     CDS             17800..18282
                     /gene="smpB"
                     /locus_tag="OO468_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR6259762.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18497..18859
                     /gene="ssrA"
                     /locus_tag="OO468_002701"
     tmRNA           18497..18859
                     /gene="ssrA"
                     /locus_tag="OO468_002701"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19064..20305
                     /locus_tag="OO468_002702"
     CDS             19064..20305
                     /locus_tag="OO468_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6259763.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5256 a         5324 c         4774 g         4991 t
ORIGIN      
        1 tggctcgctt tgagatattc ctcaagtaaa aaaacacctc ttcctgcgat ttctcacaaa
       61 aaagattcgt tgacaaaaag tgacaaaatt atgagatttc catcacacat tttgacatca
      121 ggaacggtat gctgaattca ccaagacggg aagacaagag gtaaaattta tgacaatgaa
      181 cattaccagc aaacaaatgg aaattactcc ggccatccgc caacatgtcg cagaccgtct
      241 cgccaaactg gaaaaatggc aaacacatct gattaatcca catatcattc tgtccaaaga
      301 gccacaaggg tttgttgctg acgccacaat caatacacct aacggcgttc tggttgccag
      361 tggtaaacat gaagatatgt acaccgcaat taacgaattg atcaacaagc tggaacggca
      421 gctcaataaa ctgcagcaca aaggcgaagc acgtcgtgcc gcaacatcgg tgaaagacgc
      481 caacttcgtc gaagaagttg aagaagagta gtcctttata ttgagtgtat cgccaacgcg
      541 ccttcgggcg cgttttttgt tgacagcgtg aaaacagtac gggtactgta ctaaagtcac
      601 ttaaggaaac aaacatgaaa cacataccgt ttttcttcgc attctttttt accttcccct
      661 gaatgggagg cgtttcgtcg tgtgaaacag aatgcgaaga cgaacaataa ggcctcccaa
      721 atcggggggc cttttttatt gataacaaaa aggcaacact atgacatcgg aaaacccgtt
      781 actggcgctg cgagagaaaa tcagcgcgct ggatgaaaaa ttattagcgt tactggcaga
      841 acggcgcgaa ctggccgtcg aggtgggaaa agccaaactg ctctcgcatc gcccggtacg
      901 tgatattgat cgtgaacgcg atttgctgga aagattaatt acgctcggta aagcgcacca
      961 tctggacgcc cattacatta ctcgcctgtt ccagctcatc attgaagatt ccgtattaac
     1021 tcagcaggct ttgctccaac aacatctcaa taaaattaat ccgcactcag cacgcatcgc
     1081 ttttctcggc cccaaaggtt cttattccca tcttgcggcg cgccagtatg ctgcccgtca
     1141 ctttgagcaa ttcattgaaa gtggctgcgc caaatttgcc gatattttta atcaggtgga
     1201 aaccggccag gccgactatg ccgtcgtacc gattgaaaat accagctccg gtgccataaa
     1261 cgacgtttac gatctgctgc aacataccag cttgtcgatt gttggcgaga tgacgttaac
     1321 tatcgaccat tgtttgttgg tctccggcac tactgattta tccaccatca atacggtcta
     1381 cagccatccg cagccattcc agcaatgcag caaattcctt aatcgttatc cgcactggaa
     1441 gattgaatat accgaaagta cgtctgcggc aatggaaaag gttgcacagg caaaatcacc
     1501 gcatgttgct gcgttgggaa gcgaagctgg cggcactttg tacggtttgc aggtactgga
     1561 gcgtattgaa gcaaatcagc gacaaaactt cacccgattt gtggtgttgg cgcgtaaagc
     1621 cattaacgtg tctgatcagg ttccggcgaa aaccacgttg ttaatggcga ccgggcaaca
     1681 agccggtgcg ttggttgaag cgttgctggt actgcgcaac cacaatctga ttatgacccg
     1741 tctggaatca cgcccgattc acggtaatcc atgggaagag atgttctatc tggatattca
     1801 ggctaatctt gaatcagcgg aaatgcaaaa agcattgaaa gagttagggg aaatcacccg
     1861 ttcaatgaag gtattgggct gttacccaag tgagaacgta gtgcctgttg atccaacctg
     1921 atgaaaaggt gccggatgat gtgaatcatc cggcactgga ttattactgg cgattgtcat
     1981 tcgcctgacg caataacacg cggctttcac tctgaaaacg ctgtgcgtaa tcgccgaacc
     2041 agtgctccac cttgcggaaa ctgtcaataa acgcctgctt atcgccctgc tccagcaact
     2101 caatcgcctc gccgaaacgc ttatagtaac gtttgattaa cgccagatta cgctctgacg
     2161 acataatgat gtcggcataa agctgcggat cctgagcaaa cagtcgcccg accatcgcca
     2221 gctcaaggcg gtaaatcggc gaagagagcg ccagaagttg ctcaagctga acattttctt
     2281 ctgccaggtg cagcccgtaa gcaaaagtag caaagtggcg cagagcctga ataaacgcca
     2341 tattctgatc gtgctcgaca gcgctaatac gatgcaaccg agcgccccag acctgaattt
     2401 gctccagaaa ccattggtat gcttccggct tacgtccatc acaccagacc acaacttgct
     2461 ttgccaggct accgctgtcc gggccgaaca tcgggtgtaa ccccagtacc gggccatcgt
     2521 gcgccgccag catggcctgt aatggtccat ttttcactga tgccagatca accagaatac
     2581 aatctttcgg taaaggcggt aatttgccga taacttgctc agtaacgtgg attggcacac
     2641 taacaatcac cattccggca tcggcaacaa tatcagccgc tcgatcccag tcatgttgct
     2701 ccagaatccg cacctgataa cccgatagtg tcagcatctt ctcgaacagg cgtcccatct
     2761 gaccgccacc gccgacgata accaccggac gcagtgacgg acaaagtgtt ttaaatcctt
     2821 tgtcgttttc actggagtaa gattcacgca tcacccgacg caaaacatcc tcaatcagat
     2881 ctggcggtac acccagagct tccgcctctg cacgacgcga ggccaacata gatgcctcgc
     2941 gctccggaac ataaataggc agtccaaagc ggcttttcac cttgcccact tcagcaacca
     3001 gttccagacg cttcgctaat aaattcagca gcgctttatc gacttcatca atttgatcgc
     3061 gtaatgcggt caattcagca accataataa acctcttaag ccacgcgagc cgtcagctgc
     3121 ccgttcagat cctgatgaat ttcacgcagc aaggcatcgg tcatttccca gctaatgcag
     3181 gcatcggtta cggatacacc gtatttcatt tcactgcgcg gttgctcaga agactgattg
     3241 ccctcgtgga tattactttc gatcatcaga ccaataattg agcgattacc atctttgatt
     3301 tgagcaacca cggattctgc caccgcaggc tgacggcgat aatctttatt ggaattaccg
     3361 tggctgcaat ctaccatcag agacgggcgc agtcccgcct gttccatctc tttttcacat
     3421 tgcgcaacat ccgcagggct atagttcggc gctttaccac cgcgcaggat cacatgcccg
     3481 tccggattcc cctgagtttg tagcaacgca acctgccctg cctggttaat gccaacaaaa
     3541 cagtgcggct gggcggcggc gcgcatagcg ttaattgctg ttgccagact gccgtcggtg
     3601 ccgtttttaa aaccaaccgg catggaaagc ccggaggcca tttcacggtg agtttgcgat
     3661 tccgttgtac gagcaccaat tgctgaccag ctaaacagat cgcccaggta ttgcgggcta
     3721 ttcggatcta acgcttccgt cgccagtggc agtcccatat tcaccagctc aagcaacaat
     3781 ttacgcgcga tttgcagccc ggcttctaca tcaaaagagc catccatatg gggatcgtta
     3841 attaaccctt tccagccgac agtggtacgg ggtttttcaa aatagacgcg cattaccaga
     3901 tagaggctat cgctgacctc tgcggcaaga gctttaaatc gacgagcata ttccagagca
     3961 gtttccggat catgaatgga acaaggacca catactacca gcagacgagg atcgcgcccg
     4021 gcgataatat ctgaaatggt tttacgcgag tcagcaatct gggcttcttg ttgcaggctc
     4081 aatggaaaag cggccttcag ttgttccgga gtcattaaaa cctgttcgtc ggtaatatgt
     4141 acgttattca gcgcgtcttt atgcatgatg gcgatcctgt ttatgctcgt ttgcgatagt
     4201 tgctcctcag cgaggatgac gtaacgataa cacataaagt aaagttttca atccatattt
     4261 cgtacatttt tatttacaca ggcaatttag tcgcgctttc aacccttgct tctgtataga
     4321 taaatttaca tcccctttaa aaacaatccg ctatgctttg aaaaaggaga aagaaatgat
     4381 gaaaaagttt atcgccccct tgttggcttt actggttagc ggatgtcaga ttgatcctta
     4441 tactcacgcg ccaaccttga ccagcaccga ctggtatgat gtcggtatgg aagatgcgat
     4501 atcgggcagc gccataaaag atgacgatgc atttagcgat tcacaggcgg atcgcggtct
     4561 ataccttaaa ggatatgccg aagggcaaaa gaaaacttgc cagaccgatt ttacttatgc
     4621 ccggggactt tccggtaaaa gctttcctgc cagctgcaac aatgttgaaa atgccagcca
     4681 actgcatgaa gtatggcaaa aaagggctga tgaaaatgcc agcacaatac gattaaatta
     4741 attaaaaccc cacaaataaa ttaaacataa gattttactc attgtgtttt atttctcaca
     4801 ttgatgacgg tcgcatgaaa aaatgatatt ttaatgagca tcctgaaaaa atatcgatgc
     4861 atttcgagcg aagatggtgg ggatccctga atgcgttttt ctcaccgact gttcttactt
     4921 ctgattctcc tgctgacggg agcaccaata ctcgctcaag agccttctga cgttgcgaaa
     4981 aacgtgcgta tgatggtttc cggtattgtc agttacaccc gctggcctgc gctatcaggg
     5041 ccaccaaagt tatgtatctt ttcatcttca cgttttagta ccgccctaca agagaatgcc
     5101 gcgacatctt tgccttatct gcctgtcatc attcacactc aacaagaggc gatgatttca
     5161 ggctgcaatg gtttttattt tggcaacgag tcaccgacat tccagatgga attaaccgaa
     5221 caatatccgt caaaggcgtt attattaatt gccgaacaaa ataccgagtg cattattggt
     5281 agcgcctttt gtctgatcat ccacaataat gacgtcagat ttgccgtaaa cctggatgcc
     5341 tcatcgcgta gcggtgtaaa ggtcaacccg gatgtcttaa tgctcgcacg gaagaaaaat
     5401 gatggataac gataattctc ttaataagcg ccccacgttt aaaagagcat tacgcaacat
     5461 cagtatgacc agcatattta tcactatgat gctgatctgg ttgctgcttt ccgtgacctc
     5521 ggtgctgacc ctgaaacagt acgcgcaaaa aaacctggca ctgacagcag caacaatgac
     5581 ttacagtctg gaagcagctg tcgtttttgc cgatggccct gcagcaactg aaacactggc
     5641 agcgctgggc cagcaagggc attttcaact gcagaagtac gtgataagca gcaaaatatt
     5701 ctggcatcct ggcattaccc ccgtaaggat ccaggcgata ctttcagcaa tttcataagc
     5761 cactggctct tccctgcccc catcattcag ccgattcgtc acaatggtga aaccattggc
     5821 gaagtacgct taaccgctcg cgacagttca atcagccatt ttatctggtt ttcgctcgcc
     5881 gtactgaccg gttgtattct gctggcatca ggcatcgcaa ttaccctcac ccgccatttg
     5941 cacaatggcc tggtggaagc actgaaaaat atcaccgatg tcgtacatga tgtgcgttcc
     6001 aaccgcaatt tttcccgacg agtttcggaa gaacgtatcg ctgagtttca ccgcttcgct
     6061 ctcgacttca acagtctgct ggatgaaatg gaagagtggc agcttcgttt acaggccaaa
     6121 aatgcgcagc ttctgcgtac cgcgctacat gacccattaa ccgggctggc taaccgcgca
     6181 gcgtttcgta gcggcatcaa tacgttgatg aacaattccg atgcccgaaa aacgtcggcg
     6241 ttactatttc ttgatggcga taatttcaaa tatatcaatg atacctgggg tcatgcgacg
     6301 ggcgataggg tcttgattga aatcgcaaaa cggttagctg aatttggcgg gctgcgacat
     6361 aaagcatacc gcctgggcgg cgatgaattc gctatggtgc tctatgatgt acagtcggaa
     6421 tctgaagtgc agcagatatg ctcagcactg acacaaatct ttaatctccc gtttgatctt
     6481 cataatggcc atcagaccac catgacatta agcattggtt acgcgatgac cattgagcac
     6541 gcctctgcgg aaaaattaca agagcttgcc gatcacaata tgtatcaggc caaacaccag
     6601 cgtgccgaaa agctggtgag ataacaagga tatatcgata tgataaagca cctggtagca
     6661 cccctgattt tcacctcact aatactgact ggctgccagt cccctcaggg aaagtttact
     6721 cctgagcaag tcgccgctat gcaatcttat ggatttactg aatccgccgg cgactggtcg
     6781 ctgggcttat cagatgccat tctgttcgca aaaaatgact acaaattgct cccggaaagc
     6841 cagcaacaga tccaaaccat ggcagccaaa ttggcctcga cagggctaac acatgcccgt
     6901 atggatggac acaccgataa ctatggtgaa gacagttaca acgaaggctt atcattgaaa
     6961 cgggcgaatg tcgtggccga tgcatgggct attggtggac aaattccacg cagcaatctc
     7021 accacacagg gtttaggaaa aaaatatccc atagccagta acaagaccgc ccagggccgc
     7081 gccgagaacc gccgcgtcgc agtggtgatt gcaacccctt aaaacacgag caaacagaca
     7141 aaaaaagggc cagccaattg gccagccctt cttaacagga tgtcgcttaa gcgaaatctt
     7201 agttaagacg ctctttgata cgagcagcct taccagtacg ctcacgcagg tagtacagtt
     7261 tagctttacg aacagcacca cgacgtttga cagaaatgct gtcaactacc ggagagtgag
     7321 tctggaagac acgctcaacg ccttcgccgt tggaaatttt acgaacagtg aatgcagagt
     7381 gcagaccgcg gttacgaata gcgataacca cgccctcgaa tgcctgcaga cgttttttgg
     7441 aaccttcaac aacccatact ttcacttcca cggtatcacc cggacggaag gaaggtacgt
     7501 cctgcttcat ctgctcttgt tcaagttgct taataatgtt gctcataatt taatctctta
     7561 tcctgggtaa actgatctct cgggggctta cgccatccca tcatgtttat gttgctgttg
     7621 tgcgtgttcc gttttgaact ccgccagcaa ccttgcttgc tcttcagtca gagccaggtt
     7681 ttccagaagt tcaggtcttc taagccaggt acggcccagc gactgtttca aacgccagcg
     7741 acgtatctcg gcatggttgc ccgacagtaa cactggcgga acttccatcc cttctaacac
     7801 ctcaggccgc gtatagtgcg ggcaatccag caatccttca gcaaaggaat cttccgttgc
     7861 cgaggcttca tgtcccagta ctcccggaat aaaccgggaa acggagtcaa tcagcgtcat
     7921 tgctggtaac tcaccaccac tgagaacgta atcgccgatt gaccattctt cgtcaatttc
     7981 ggtttggatc acgcgctcat ctataccttc gtagcgaccg cacaccagaa tcaatttttg
     8041 attcgttgcc agttcgctga cgcccgcttg atcaagcttg cgtccctgtg gtgacagata
     8101 aatcaccttt gcgccttcac ccgccgcggc ttttgctgca tgaatggcgt cccgcaaggg
     8161 ttgcaccatc attaacatcc ccggtccgcc gccgtaagga cgatcgtcca cggtacggtg
     8221 ccggtcatgc gtgaagtcgc gaggactcca actctggatg ctcagcaggc catttttaac
     8281 tgcccggcca gttaccccgt aatcggtaat tgcgcggaac atttcaggaa acaggctaat
     8341 tatgccaatc cacatagcgc cgccttttac cgtttatccg gtggtttaaa aaccaggatc
     8401 ccaatctact tcgattgaac gagtagtgag atcgactttc ttgataacct gcccatcgag
     8461 gaacggtacg agacgttcct tgataccaaa cgcatctttc aggtttgcct taatgacgag
     8521 aacgtcatta gatccggttt ccatcatatc aacgacttta ccgagatcgt agccttcagt
     8581 ggttactacc tggcagccca tcaggtcttt ccagtagtag tccccctctt caagctgagg
     8641 cagctgcgat gaatccacga caatttcaca attcgtcagc aggttcgccg catcacgatc
     8701 gtcaacgcct ttcagcttga tgatcatgtc ctgattgtgg tgcttccagc tttccagctg
     8761 gacttgctgc cactgacccg ccttctggat aaaccagggc tgatagtcaa aaatgctttc
     8821 ggcgtcttcg gtggaagaaa acactctgag ccacccacga ataccgtaag acgaacccat
     8881 ttttcccaaa acgatgggat caacaggtgc ttgcgcggtg agttgtttgc tcatcatgac
     8941 caccgtgaca gattaagctg ctttgtttac ttctttgatc agcgcagcaa cgcgatcaga
     9001 aatagttgcg ccctggccaa cccagtgagc gatgcgatcc agatccaggc gagtgccttc
     9061 ttctttttcg ctagcgattg ggttgaagaa accaacgcgc tcgatgaagc gaccgttgcg
     9121 tgcattacgg ctgtcagcga caacaacctg gtagaacgga cgctttttag cgccgtgacg
     9181 tgctaaacga atagttacca taacatcctc ttgtgtgaat aaaacaaccg gaccccatcg
     9241 aggaacggag tccggtgtca tattaaaagc ccgaaaattt tactgatttc tggagaaaat
     9301 gcaatcagca gttaataact ctgtcgcata aggtcattta gcgaccagga aatcccggcg
     9361 gcatcattcc cttcatgctt ctcatcatct tcgccattcc gcctttcttc attttcttca
     9421 tcatgcgctg catgtcgtcg aactgcttca gaaggcggtt aacgtcctgt acctgcatcc
     9481 cgcaaccggc ggcgatacga cgcttacgcg aacctttgat gatttctggc ttagcgcgct
     9541 ctttcatcgt cattgagttg atgatggctt ccatacgcac cagcacttta tcgtccatct
     9601 gcgacttgac gttatccggg atctgcccca tgcccggcag tttgcccatc aagctggcca
     9661 tgccgcccat atttttcatc tggcgcagtt gctcaagaaa gtcgttgaga tcgaagccgt
     9721 cgcctttttt tagcttgctg gctaatttct ctgcctgcgc gcggtcaact ttgctttcga
     9781 tatcttcgat cagcgacagt acgtcgccca tgccgagaat acgcgacgcg atgcggtccg
     9841 gatggaacgg ctccagcgcc tcagttttct cgccaacacc gaggaacttg atcggtttgc
     9901 cagtgatgtg acgaatagag agcgccgcac cgccgcgggc atcgccgtcc actttggtca
     9961 acactacgcc ggtaagcggt aacgcttcat tgaatgcttt tgccgtattg gccgcatcct
    10021 gaccggtcat ggcgtcaacc acaaacaggg tttcaaccgg gttaatcgac gcatggactt
    10081 gtttgatctc gtccatcatc gcttcgtcaa cgtgcagacg accagcggta tccaccagca
    10141 gcacgtcgta gaatttcagt ttggcttctt tcagcgccgc gttaacaata tcaaccggct
    10201 tctgaccaac atcagaaggg aagaaatcaa cgcccacctg ctctgccagc gtctcaagct
    10261 gtttgattgc cgccgggcga tagacgtcag cagaaaccac cagcactttc ttcttgtgct
    10321 tctcgcgcag gaaattaccg agtttaccaa cgctggttgt tttaccggca ccttgcaggc
    10381 ctgccatcag cacgaccgca ggcggttgcg cagccagatt cagggtctgg ttctcttcgc
    10441 ccatcgccgc aaccagttcg ttacggacga ttttgacgaa ctcctgcccc ggcgtcaggc
    10501 tcttattaac ttcatgacca accgctttct cttttacgcg attgataaac tcacgcacta
    10561 ccggcagagc gacgtccgcc tccagcagcg ccatgcgcac ttcgcgcagc gtatctttta
    10621 cgttgtcttc agtgaggcgt ccacggccac tgatattgcg cagcgtgcgc gacaaacgat
    10681 cggttaaatt atcaaacatt gtctctcgcc tggggtggaa acggttggcc gcaatcgcga
    10741 cacatcatca gtattttgcc gcagtataac atgaaggcgt ctttgttgtt atgcaacggt
    10801 tggagcagcg ttcacctgac gctatactgc ttctctttct tattgctcaa actgtcgaca
    10861 ttactatgcc cgtttttgct ctgctcgcgc ttgtcgccta ctccgtcagt cttgcgctga
    10921 ttgttcccgg tctgctgcaa aaaaacggcg gctggcggcg catggctatt atttctgcgg
    10981 tcattgcgct ggtctgccac gcaatcgctc tggaagcccg catcctgccc gacggtgata
    11041 gcggacaaaa cctcagcctg ctgaacgttg gttcattggt cagtttggtg atctgtacgg
    11101 taatgaccat tgtggcttct cgcaatcgtg gctggttgct gctacccatt gtctatgcct
    11161 ttgcgcttat caacctggcg ctggcgacct tcatgcccaa tgaatacatc acccatctgg
    11221 aagcaacgcc tgggatgctg atgcacattg gcttatcgct cttttcctat gccacgctaa
    11281 ttatcgccgc cctgtacgcg ctgcaactgg cgtggattga ttaccaactg aagaacaaga
    11341 agctggcatt taaccaagaa atgccgccat tgatgagtat cgagcgtaaa atgttccaca
    11401 tcacgcagat tggcgtggtg ctgctaacgc tcacgctttg cactggcctg ttctacatgc
    11461 acaacctatt tagcatggaa aatatcgaca aggctgtgct ctctatcgtg gcgtggtttg
    11521 tctatattgt gctgctgtgg ggacattacc atgaaggatg gcgtggacgc cgcgtcgtct
    11581 ggtttaacgt tgcgggcgcg gtaattctga cactggccta cttcggcagc cgaattgtcc
    11641 agcagttaat cagctaaacc cagaaaagga gtttcccctg gaacacattt ctactactac
    11701 gttgatcatt attctgatca tcatggtggt catttcagcc tatttttccg ggtccgaaac
    11761 cggaatgatg accctcaacc gctatcgtct tcgacatatg gcgaaacagg gtaatcgctc
    11821 ggccaaacgc gtcgaaaaat tgctgcgtaa gccagaccgc ctgataagcc tggtgttaat
    11881 cggcaataac ctggtcaata ttcttgcctc cgcgctcggc actattgtcg ggatgcgttt
    11941 gtacggcgat gcgggcgtgg caattgcgac tggtgtgctg acttttgtcg tactggtttt
    12001 tgctgaggta ttgccgaaaa ccattgccgc gctgtacccg gaaaaagtcg cttatccgag
    12061 tagttttctg ctggctccgc tgcaaatttt gatgatgccg ctggtctggt tgctgaatgc
    12121 tatcacccgt atgctgatgc gcatgatggg tatcaaaacc gatatcgtgg ttagcggctc
    12181 tttgagcaaa gaagagttgc gcactatcgt gcatgaatcg cgctcacaaa tttcccgtcg
    12241 caatcaggat atgctgctgt cggtgctcga tctggaaaaa atgaccgttg atgacatcat
    12301 ggtaccgcgc agtgaaatta tcggtattga tatcaacgat gactggaaat ctattctgcg
    12361 ccaactctcc cactcacctc acgggcgcat cgtgctttac cgtgattcgc tggacgacgc
    12421 catcagtatg ctgcgagtac gtgaagcctg gcggttgatg tcggagaaaa aagagttcac
    12481 caaagaaacc atgctgcgcg ccgcggacga gatctatttt gtgcctgaag gtacgccgct
    12541 cagcacgcag ttggtaaagt ttcagcgcaa caaaaagaaa gtcggcctgg tcgtcaacga
    12601 gtatggagac attcaggggc tggtgacggt tgaagatatt ctggaagaga ttgttggcga
    12661 tttcactacg tcgatgtcgc caacacttgc cgaagaggtc acgccgcaaa acgacggttc
    12721 ggtgattatc gatggcaccg ccaacgtgcg ggaaatcaac aaagccttta actggcatct
    12781 accggaagat gatgcccgca cggttaatgg cgtcattctt gaggcactgg aagagatccc
    12841 tgtcgcaggc acccgcgtgc gtattggcaa gtacgatatc gatattctcg acgtacagga
    12901 caatatgatt aagcaggtaa aagtttttcc tgtgaaaccg ctgcgcgaga gtgtggcgga
    12961 gtaacgaaaa cggccctgca ttcgcatgca gggccgtgaa ttattacgaa aacagaaatt
    13021 acgcttttgc tttcgctaca gttaccatcg ccgcacgaat cgtacgacca ttcagcgtat
    13081 aacccttctg cataatgccc agtacgttac ctggcgcaac gtcatcagat tccaccattg
    13141 cgatagcctg atgcacattc gggtccagcg gcacgttagt ttcggcgatc acttcaacac
    13201 caaacttacg cacaacatcc agcatcgact tcagcgtcag ctcaatgcct tcaaccatcg
    13261 cagacatatc cgggttagct ttatcagcca cttccagcgc acgatccagg ctatcaatca
    13321 ccggcagcaa ttcgttgatg aatttctcca gcgcgaattt gtgggctttt tcaatatcca
    13381 gttcagtacg acgacgcagg ttttccattt cggcttttac gcgcaaaata ccgtcacgtt
    13441 cacgggtctg ggcttcagcc agctgagctt cgagattcgc aattttttca tcgcgcggat
    13501 ccacctgctc agcagaagct tctggctcaa ctgcctcaat ctcttcgtgc tgatccatga
    13561 taatttcttc cggggcttgc ccctcaggcg ttttctgttc tttactactc atgaatttct
    13621 ccgcgttttt ttcgcattca tctcgctaac ttcgcttatt atggggatca gtttcagggt
    13681 ttcaagggaa gcactcacat tgtcatcaat cttcgcaaca aggacctcgg aaaaatgaat
    13741 aatcatttca agtgtattgg cattgtggga cacccacggc accctactgc actgacaaca
    13801 catgaaatgc tctaccgctg gctgtgcaca aaaggttacg aggtcatcgt tgagcaacaa
    13861 atcgctcacg aactgcaact gaagaatgtg aaaactggca cgctcgcgga gattgggcaa
    13921 ctggctgatc tcgcggtagt cgttggtggc gacggtaata tgctgggcgc ggcgcgcacg
    13981 ctcgcccgtt acgatattaa agttattgga atcaaccgtg gcaacctggg tttcctgact
    14041 gaccttgacc ccgataacgc ccagcaacaa ttagccgatg tgctggaagg tcactacatc
    14101 agcgagaaac gttttttgct ggaagcgcaa gtctgtcagc aagattgcca aaaacgcatc
    14161 agcaccgcga ttaacgaagt ggtacttcat cctggcaaag tggcgcatat gatagagttc
    14221 gaagtgtata tcgacgagat ctttgcgttt tctcagcgat ctgatgggct aattatttcg
    14281 acgccaacag gctccaccgc ctattccctc tctgcgggcg gtcctattct gaccccctct
    14341 ctggatgcga ttaccctggt gcccatgttc ccgcatacgt tgtcagcacg accactggtc
    14401 ataaacagca gcagcacgat ccgtctgcgt ttttcgcatc gccgtaacga cctggaaatc
    14461 agttgcgaca gccagatagc actgccgatt caggaaggtg aagatgtcct gattcgtcgc
    14521 tgtgattacc atctgaatct gattcatccg aaagattaca gttatttcaa tacattaagc
    14581 accaagctcg gctggtcaaa aaaattattc taattttacg ccagcctctt tactgtatat
    14641 aaaaccagtt tatactgtac acaataacag taatggtttt tcatacagga aaacgactat
    14701 gttggcacaa ctgaccatca gcaactttgc tatcgttcgt gagcttgaga ttgattttca
    14761 tagcggcatg accgtaataa ctggcgagac cggtgcgggt aaatctattg caatagatgc
    14821 cctcggtctt tgtctcggtg gtcgcgctga agccgacatg gtgcgtaccg gcgctgctcg
    14881 cgctgacctg tgcgcccgtt tttctctgaa agatacgcca gcggccctgc gctggctgga
    14941 agaaaaccag cttgaagacg ggcatgaatg tttgcttcgt cgcgtgatca gcagtgatgg
    15001 tcgctcccgt ggtttcatca acggtacagc tgttcctctg tcacaactgc gcgaactggg
    15061 tcagttgctg attcagatcc atggtcagca cgctcatcaa ttactcacca aacctgagca
    15121 ccaaaaattc ctgcttgatg gctatgccaa tgaaacctct ctacttcagg aaatgaccgc
    15181 acgttatcag ttgtggcatc aaagctgccg tgacctcgcg catcatcaac agttaagtca
    15241 ggaacgcgcc gcccgtgcgg aactgctgca ataccaatta aaagaactta acgaatttaa
    15301 tccgcagccc ggagagtttg agcaaatcga cgaagagtac aaacgtctgg cgaacagcgg
    15361 tcaattgctg accaccagcc agaatgcatt ggcattaatg gccgacggtg aagacgcaaa
    15421 cctgcaaagt cagctttaca cggctaaaca actggtgagc gaattgattg gcatggacag
    15481 caaactgtcc ggcgtacttg atatgctgga agaagctacc atccagattg ctgaagccag
    15541 cgacgaactg cgccactact gcgatcgtct ggatctcgat cccaaccgac tgtttgaact
    15601 tgaacagcgc atctcaaaac agatttcgct ggcacgtaaa catcacgtca gccctgaggc
    15661 attaccacag tattaccagt cgctactgga agaacagcag caactggacg atcaggccga
    15721 ctcacaagaa acgctcgcgc tggcggtaac gaaacatcat cagcaggcac tggaaaccgc
    15781 gcgcgcatta caccaacaac gccagcaata tgcagaagaa cttgcacagc tgatcaccga
    15841 cagtatgcat gcgctctcaa tgccgcatgg gcagtttacg attgatgtca aatatgacga
    15901 gcatcacctg ggcgctgacg gtgccgatcg tattgagttt cgggtaacca ccaacccagg
    15961 tcagccaatg cagcctattg ccaaagtcgc atccggtggt gaattgtccc gcatcgcact
    16021 ggcaatccag gtcatcacgg cgcgtaaaat ggaaaccccg gcactgattt ttgatgaagt
    16081 ggatgtaggg attagcggtc caacagcggc agttgtcggc aaactgctgc gtcaactcgg
    16141 cgaatcaact caggtgatgt gtgttaccca cctgccacaa gtcgcgggat gtggtcatca
    16201 acactatttt gtcagcaaag aaaccgatgg tgcgatgaca gaaacacata tgcaatctct
    16261 ggataaaaaa gcgcggttac aagagctggc gcgcctgctt ggtggcagtg aagtcacacg
    16321 taacacactg gcgaatgcga aagaactgct tgcagcgtaa acttttttcc tgcttcacgg
    16381 tcagagtaaa cagcaaaacg ccgtaagacc ggaaagcaaa aggttttaaa gtgatgaaag
    16441 gtctattatc atcggcatat tacagatgag ccacgtactg ctcgggcccg aaaaggaatc
    16501 aaatcactat gcgctgtaaa acgctgactg ctgcagcagc agtactattg atgttaaccg
    16561 caggctgttc cactctggag cgagtggttt accgtcctga catcaaccag gggaactatc
    16621 tgaccgctaa cgacgtatcc aaaatacgtg ttggcatgac gcaacaacaa gttgcgtacg
    16681 cattgggtac accgctgatg tccgatccat ttggtacgaa tacctggttc tatgtcttcc
    16741 gccagcaacc aggtcatgaa ggtgtaacgc agcaaacgct gacgctgacc tttaacagta
    16801 gcggtgtgtt gaccaatatt gataacaaac ctgcgctgag tggtaactaa taataaagtt
    16861 gctctcaaag acgttaaaaa aggtgctcaa tgagcacctt ttttctgtct gttatttatt
    16921 cgctgatttt tctgctcgtt gcctgcgaag ctctttcgga tcggcaatga gagggcgata
    16981 aatctccacc cgatcgccat catgcacact atcgcttagt tttgccggac ggctgtaaat
    17041 gccgactttg tttttagtta aatcgatatc ggtacgcaat tccagcaagc cactggcgcg
    17101 aatagcttct tcaaccgtcg cgccctcctg cagcgtcact cgctgcaggt attgcttctc
    17161 aggtagcgca taagccacct caacggcaat tttacctagc actgtaaacc tctttcgcac
    17221 gaaccgtaaa agcctggacc atattagccg ccagctcttt aaacacgcga ccaaaggcga
    17281 gttcaatcaa cttattggta aactcaaagt cgagatgaaa ctcgatacga cacgcctcct
    17341 ggctcagcgg cgtaaacttc catccaccaa tcaatttctt gaacggccca tccaccagat
    17401 tcataagaat actttggtta ctggtcaact ggttgcgggt agtaaacgtt ttgctgatcc
    17461 cagccttaga gacatctacc gccgcagtca tctgcccagg agtggactcc agaatccgac
    17521 ttccggtaca acctggcaaa aactgaggat aagactgaac gtcattcact aactgataca
    17581 tttgctccgc gctgtagggt accagtgcgg tccggctaat ctgaggcata acaatttcca
    17641 tcaacaaaaa tccaacaaat aatatcattt atcctgctgt aaaaaaaacg ctatcccggc
    17701 gctgggtaac atcgggttca tgctaagata gagccttgtc ccccgcagga ttgatatggg
    17761 gtattttcga tttcagatta ccgatgattc acgacgctta tgacgaagaa aaaagcacat
    17821 aaacctggtt cagcgaccat cgcgcttaac aagcgcgccc gtcacgaata ctttatcgaa
    17881 gaagagttcg aagcgggact tgccctgcaa ggctgggaag ttaaatccct gcgcgcagga
    17941 aaagccaata tcagcgacag ctacgtcctt ctgcgtgacg gagaggcatt tctgtttggc
    18001 gctaacatca cgccaatggc cgtggcctcc acgcatgtgg tgtgcgatcc tacccgtacc
    18061 cgcaagttac ttctcaacca gcgcgaactg gactcattgt acggtcgcgt caatcgagaa
    18121 ggctataccg tagtggcgct ctccctgtac tggaaaaatg cctggtgcaa agtgaaaatc
    18181 ggcgtcgcca aaggtaaaaa acagcacgat aaacgttcag atatcaaaga acgcgaatgg
    18241 caggtggata aagcacgcat catgaaaaac gcccaccgtt aaacctgcac tccaattatt
    18301 gaccagttcc tcaccgcgcc tccctctccg gcggcgcgaa tgaacatctt attggctatc
    18361 acatccgaca caaatgttgc catcccattg cttaatcgaa taaaaatcag gctacatggg
    18421 tgctaaatct ttaacgataa cgccattgag gctggtcatg gcgctcataa atctggtata
    18481 cttaccttta cacattgggg ctgattctgg attcgacggg atttgcgaaa cccaaggtgc
    18541 atgccgaggg gcggttggcc tcgtaaaaag ccgcaaaaaa tagtcgcaaa cgacgaaaac
    18601 tacgctttag cagcttaata acctgcttag agccctctct ccctagcctc cgctcttagg
    18661 acggggatca agagaggtca aacccaaaag agatcgcgtg gaagccctgc ctggggttga
    18721 agcgttaaaa cttaatcagg ctagtttgtt agtggcgtgt ccgtccgcag ctggcaagcg
    18781 aatgtaaaga ctgactaagc atgtagtacc gaggatgtag gaatttcgga cgcgggttca
    18841 actcccgcca gctccaccaa aattctccat cggtgattac cagagtcatc cgatgaagtc
    18901 ctaagagccc gcacggcgca agccctgcgg gcttttttgt gctccgaatt tgtcccgcga
    18961 agtctgatgc caactaatta aatccgatcc ttttaggcac cttgttaggc acttcataaa
    19021 gctatattgt ctttgaggtg cctaaaacta tggaaacccg gcaatggcaa gacaaaccaa
    19081 acctctatcc gttaaggaaa tcgaatctgc taaacccaag gaagcggact acgttctcta
    19141 tgatggcgat ggccttgagc tactcattaa atccagcggg agtaaaatct ggcagtttcg
    19201 ctacattcgc cctgtcacca agacacgagc aaagaaaagc ataggccctt acccgtcagt
    19261 tacccttgcc gatgccagaa actaccgtgc agagtcccgc tcactcctag ctaaacagat
    19321 tgatccgcag gaacatcagc aagaacaatt gcgtagttcg ctggaagcca aaaccaatac
    19381 tttccagctc gtagctgaac gttggtggaa tgtgaagaaa gccagtgtga cagaggacta
    19441 tgcggaagat atctggcgct ctcttgaaag agatgtcttt cctgcgattg gcgacgttag
    19501 cgttacagat attaaagctc atacactggt tcaggccgta caaccggttc aggccagagg
    19561 agcactggaa acggttcgtc gcctgtgtca acgtattaat gaggtcatga tctatgccca
    19621 gaacacaggg ctgattgatg ctgttcccag cgttaatatc ggtaaagcct tcgaaaagcc
    19681 tcagaaaaag aacatgccca gcattcgacc ggatcagcta tctcaactga tgcagacaat
    19741 gcgaacagcc agcattagcc tttccacacg ctgcctgttc atgtggcaac ttcttactat
    19801 tacccgccct gccgaagcgg ctgaagctcg ctgggaagaa gtagacatag aagcgcaaga
    19861 gtggaagatt cccgcagcac gcatgaaaat gaaccgcgac catactgttc cattgtcaga
    19921 tgaagcaata gctgtgctgg agatgatgaa accgctaagc ggcaaccgag aatttatctt
    19981 tcctagccgt atcaagccca accagcctat gaacagtcag actgttaacg cctcactaaa
    20041 acgcgcaggt tttggtggtg tgcttgtttc gcatggtctg agatcaattg ccagtacggc
    20101 tcttaatgaa caaggctttc cgcctgatgc cattgaggca gcattggccc atgtggataa
    20161 gaatgaggtt cgtcgtgctt ataaccgcag cgattacctg gaacagcgtc gcccgatgat
    20221 gcaatggtgg gcaaattttg tgatggcagc ggatcgtgga agcatgattg aaggtgggat
    20281 aaaaggaatg aagctagttg ggtgactaaa tagctgcgcg gcatagtgtc ccactttcag
    20341 ccagc
//
LOCUS       DAJTUP010000073        20097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTUP010000073 DAJTUP010000000
VERSION     DAJTUP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.073"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            82..351
                     /locus_tag="OO468_002703"
     CDS             82..351
                     /locus_tag="OO468_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR6259764.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO468_002704"
     CDS             345..710
                     /locus_tag="OO468_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR6259765.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO468_002705"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO468_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR6259766.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO468_002706"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO468_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR6259767.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO468_002707"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO468_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR6259768.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO468_002708"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO468_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR6259769.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO468_002709"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO468_002709"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR6259770.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO468_002710"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO468_002710"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR6259771.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO468_002711"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO468_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO468_002712"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO468_002712"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR6259772.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO468_002713"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO468_002713"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR6259773.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14850..16448
                     /gene="rtcR"
                     /locus_tag="OO468_002714"
     CDS             14850..16448
                     /gene="rtcR"
                     /locus_tag="OO468_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR6259774.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16430..17188)
                     /locus_tag="OO468_002715"
     CDS             complement(16430..17188)
                     /locus_tag="OO468_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR6259775.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO468_002716"
     CDS             complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO468_002716"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR6259776.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO468_002717"
     CDS             complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO468_002717"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR6259777.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18596..>20097
                     /gene="glpD"
                     /locus_tag="OO468_002718"
     CDS             18596..>20097
                     /gene="glpD"
                     /locus_tag="OO468_002718"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR6259778.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
BASE COUNT         4705 a         5547 c         5099 g         4746 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaatttat tgtttctaaa
    14761 aagattatat ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg
    14821 aaatatcgcc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    14881 gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac
    14941 tctgtatata ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc
    15001 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag
    15061 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    15121 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    15181 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt
    15241 atctacccgc ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc
    15301 caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    15361 ttgccgagga acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc
    15421 cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga
    15481 ttctgcttaa cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt
    15541 taaaacaggc acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    15601 gcggcgatac cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    15661 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    15721 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct
    15781 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    15841 tgcgcgattt gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga
    15901 tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga
    15961 acctggatta tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca
    16021 ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta
    16081 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    16141 tcactctgga cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc
    16201 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    16261 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    16321 gacgacagct ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    16381 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    16441 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg
    16501 tgtagacggc atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt
    16561 ttgaatggtc aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac
    16621 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca
    16681 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    16741 cgccatcgcg gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca
    16801 tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca
    16861 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    16921 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    16981 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca
    17041 ggatcaggtt ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg
    17101 agaagtgctc caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    17161 taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc
    17221 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct
    17281 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    17341 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    17401 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    17461 aatttttgct gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    17521 ccgctaccga ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag
    17581 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    17641 ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt
    17701 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    17761 cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    17821 cctgccagcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    17881 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    17941 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    18001 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    18061 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc
    18121 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    18181 gcgccgcccc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    18241 cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    18301 gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    18361 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    18421 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    18481 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    18541 taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga
    18601 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    18661 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    18721 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    18781 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc
    18841 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    18901 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    18961 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    19021 tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa
    19081 aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg
    19141 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    19201 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc
    19261 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca
    19321 aaagcaggcc tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat
    19381 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt
    19441 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    19501 gcagctaagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    19561 tgagtccgac tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa
    19621 cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    19681 agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa
    19741 agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct
    19801 gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg
    19861 cagcaacagc gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt
    19921 cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg
    19981 caccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    20041 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtc
//
LOCUS       DAJTUP010000074        19862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTUP010000074 DAJTUP010000000
VERSION     DAJTUP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.074"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(53..259)
                     /locus_tag="OO468_002719"
     CDS             complement(53..259)
                     /locus_tag="OO468_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR6259779.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            572..661
                     /gene="kdpF"
                     /locus_tag="OO468_002720"
     CDS             572..661
                     /gene="kdpF"
                     /locus_tag="OO468_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR6259780.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            661..2334
                     /gene="kdpA"
                     /locus_tag="OO468_002721"
     CDS             661..2334
                     /gene="kdpA"
                     /locus_tag="OO468_002721"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR6259781.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2357..4404
                     /gene="kdpB"
                     /locus_tag="OO468_002722"
                     /pseudo
     CDS             2357..4404
                     /gene="kdpB"
                     /locus_tag="OO468_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4413..4985
                     /gene="kdpC"
                     /locus_tag="OO468_002723"
     CDS             4413..4985
                     /gene="kdpC"
                     /locus_tag="OO468_002723"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR6259782.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4978..7658
                     /gene="kdpD"
                     /locus_tag="OO468_002724"
                     /pseudo
     CDS             4978..7658
                     /gene="kdpD"
                     /locus_tag="OO468_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            7655..8340
                     /gene="kdpE"
                     /locus_tag="OO468_002725"
                     /pseudo
     CDS             7655..8340
                     /gene="kdpE"
                     /locus_tag="OO468_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8615..8719
                     /locus_tag="OO468_002726"
     CDS             8615..8719
                     /locus_tag="OO468_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR6259783.1"
                     /translation="MENNNRLMPHIRRTTHIMKFTHRNSFDFHFFNAR"
     gene            9030..11227
                     /gene="speF"
                     /locus_tag="OO468_002727"
                     /pseudo
     CDS             9030..11227
                     /gene="speF"
                     /locus_tag="OO468_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11224..12543
                     /gene="potE"
                     /locus_tag="OO468_002728"
     CDS             11224..12543
                     /gene="potE"
                     /locus_tag="OO468_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR6259784.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12599..14239)
                     /gene="pgm"
                     /locus_tag="OO468_002729"
     CDS             complement(12599..14239)
                     /gene="pgm"
                     /locus_tag="OO468_002729"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR6259785.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14265..14810)
                     /gene="seqA"
                     /locus_tag="OO468_002730"
     CDS             complement(14265..14810)
                     /gene="seqA"
                     /locus_tag="OO468_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR6259786.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            14995..15759
                     /gene="ybfF"
                     /locus_tag="OO468_002731"
     CDS             14995..15759
                     /gene="ybfF"
                     /locus_tag="OO468_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6259787.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15899..16192
                     /gene="ybfE"
                     /locus_tag="OO468_002732"
     CDS             15899..16192
                     /gene="ybfE"
                     /locus_tag="OO468_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR6259788.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16332..16862
                     /gene="fldA"
                     /locus_tag="OO468_002733"
     CDS             16332..16862
                     /gene="fldA"
                     /locus_tag="OO468_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR6259789.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17072..17158
                     /gene="uof"
                     /locus_tag="OO468_002734"
     CDS             17072..17158
                     /gene="uof"
                     /locus_tag="OO468_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR6259790.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17151..17597
                     /gene="fur"
                     /locus_tag="OO468_002735"
     CDS             17151..17597
                     /gene="fur"
                     /locus_tag="OO468_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR6259791.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17681..18007)
                     /locus_tag="OO468_002736"
     CDS             complement(17681..18007)
                     /locus_tag="OO468_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259792.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18044..19450)
                     /gene="chiP"
                     /locus_tag="OO468_002737"
     CDS             complement(18044..19450)
                     /gene="chiP"
                     /locus_tag="OO468_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR6259793.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         4577 a         4992 c         5279 g         5014 t
ORIGIN      
        1 tgaacaccta aaccagcccc ctgatacgag gctggcttaa gcgcgcaggc aatcagtaaa
       61 aatcaccggt tgccttttcg gcctgatcca tccacaccgg tttatcgctg gttttcgacc
      121 agacgcggtg caggtagctg taaaagcggg cgcggtcttt ccagaaaagc atgaaaggca
      181 gcgccagaac gcccgctgca accgcatatg tacggcgtaa aaagataagc catgcaggat
      241 attctctgta gagttccata cttttctccc ccttttgtat ctacccggtg aatagcaccg
      301 gaaaaatgaa tttgtttatc tgatgaaaat agtaccgcct tttgtgtaat tttactactc
      361 atccgaccac ttatttttgc ttattgatgg tttatttaca ttcacactgt aattaaatta
      421 cacaaaagtt aaattaatac taaccatcag ttaaatcatg gcctttgcca tttttatact
      481 ttttttacac cccgtccgca gatttttgcg aaatctttgc agccagaatt ctactcttcc
      541 ggtatcactt ttaggccact ggaggtgcac tgtgagtgca ggcgtgatag ccggcgtgtt
      601 gctggtgttt ttattactgg gctatttggt ttatgccctg atcaatgcgg aggcgttctg
      661 atggctgcgc aagggttctt actgattgcc acgtttttac tggtattaat ggtgctggcg
      721 cgtcctttag gcagcgggct ggcgcggctg attaatgaca ttcctcttcc aggtacagcg
      781 ggcgttgaac gtatactctt tcgtttacct ggcgtctctg accatgagat gaactggaag
      841 caatatcttt gtgccattct cggcctgaac atgctggggc tggcggtgct gttttttatg
      901 ttgctcggtc agcactatct gccgcttaat ccacagcagt tgccggggct gtcgtgggat
      961 ctggcgctga ataccgccgt cagctttgtc acgaatacca actggcaatc ttatagcggt
     1021 gaaaccacgt tgagctattt cagccagatg gcaggcttaa cggtacaaaa ctttctttct
     1081 gccgccagcg ggattgcggt gatttttgcc ttcatccgtg cgtttactcg ccagagcatg
     1141 agcacactcg ggaatgcctg ggtcgatctg ctacgcatca cgttatgggt gctagtccct
     1201 gtggcgttgt tgatcgcact gttttttatt caacaaggtg cgcagcaaaa ctttctgccc
     1261 tatcaggcag tgaataccgt tgaaggagcg caacagctgt tacccatggg accagtagct
     1321 tctcaggaag cgatcaagat gctcggtact aacggcggtg gcttctttaa tgccaactcg
     1381 tcgcatccgt ttgaaaaccc aaccgcactg accaacttcg tgcagatgct ggcgatcttc
     1441 ttgatcccaa cagcgctgtg ctttgccttt ggtgaagtga cgggcgatcg ccgccagggg
     1501 cgcatgttgc tgtgggcgat gtcagtgatt tttgtcatct gcgtaggcgt ggttatgtgg
     1561 gcagaagttc agggtaatcc tcatctgctg gcgctcggcg cagacagcag catcaatatg
     1621 gaaggtaaag agagccgttt cggcgtgctg gtcagtagcc tgtttgcggt cgtgactacg
     1681 gcggcgtcct gtggcgcggt gattgcgatg catgattcgt ttaccgctct cggcggcatg
     1741 gtgccgatgt ggctaatgca aattggtgaa gtggtgttcg gcggtgtcgg ttctggtctt
     1801 tacggcatga tgctgtttgt cctgctggcg gtgtttattg ccgggctgat gattggtcgt
     1861 acaccggaat atctgggtaa aaaaatagac gtacgcgaga tgaaactgac tgcactggcg
     1921 attctggtta ctccaacact ggtactgatg ggcgcggcgt tggcgatgat gaccgacgcc
     1981 ggacgtagcg ccatgcttaa ccctagcccg catggtttta gcgaagtgct gtacgccgtg
     2041 tcgtccgccg ctaacaacaa cggcagcgcc tttgccggat taagcgccaa ctctccgttc
     2101 tggaactgtt tactggcgtt ctgcatgttt gtcggtcgct tcggggtgat tatcccggtg
     2161 atggcaattg ccggttcgct ggtgagtaaa aagagccaac ccgccagctc cggcacgctg
     2221 ccaacgcacg gcccgctgtt tgttggcctg ttaatcggca ccgtgttgct ggttggcgca
     2281 ctgaccttta tccctgccct ggcgcttggt ccggtggcgg aatatctctc ctgatgatat
     2341 tgagtgagca ctgaatatga gtcgtaaaca actggcgcta ttcgaaccaa cacttgtcgt
     2401 tcaggcgctg aaagaagcgg tgaaaaaatt aaacccgcag gcgcaatggc gcaatccggt
     2461 gatgtttatc gtctggatcg gcagtctgct gaccacctgt attagcatcg cgatggcaag
     2521 cggtgcgatg cctggcaatg cgctgtttag cgcggcaatt agcggctggc tgtgggtcac
     2581 cgtactgttc gctaatttcg ccgaggcgct ggcagaaggc cgcagtaaag cgcaggccaa
     2641 cagtcttaaa ggggtgaaaa aaactgcctt tgcccgcaag ctgcgtgagc cgaaatatgg
     2701 cgctgcggcg gacaaagttc ctgccgatca actgcgtaaa ggtgacatcg tgctggtaga
     2761 agctggcgat attatcccct gcgatggtga agtcattgaa ggcggcgcat cggtcgatga
     2821 aagcgccatc accggggaat cggcaccggt gatccgtgaa tccggcggcg attttgcctc
     2881 cgttaccggc ggcacgcgta ttctttctga ctggctggtg attgagtgta gcgttaaccc
     2941 cggtgagaca tttctggatc ggatgatcgc gatggtggaa ggcgcacagc gacgcaaaac
     3001 accgaacgag attgccctga ctatcctgct gattgctctg accatcgtct ttttactggc
     3061 aaccgccacg ctgtggccgt tttccgcgtg gggcggtaat gccgtgagtg taacggtact
     3121 ggtggcgctg ctggtctgtc tgatcccaac cactattggt ggcctgttgt cagcgatcgg
     3181 cgtcgccggg atgagtcgga tgctgggcgc gaatgtgatt gccaccagcg gacgtgcagt
     3241 tgaagcggca ggtgacgttg acgttctgct actggataaa accggcacca tcacactcgg
     3301 taaccgtcag gcgtcggagt ttatccccgc gcagggcgtg gatgaaaaaa cgctggctga
     3361 cgccgcacaa ctggcttcgc tggctgatga aacgccggaa ggtcgcagta ttgtgatcct
     3421 cgccaagcaa cgttttaacc tgcgcgagcg cgatgtgcag tcgctccatg ccacctttgt
     3481 accgtttact gcgcaaagcc ggatgagcgg gatcaacatc gacaaccgca tgatccgtaa
     3541 aggttctgtc gatgccattc gccgccatat tgaggctaac ggcggtcact tccctgccga
     3601 tgttgatcaa aaagtcgatc aggttgcgcg tcaaggagcc acgctgctgg tggtggtgga
     3661 aggttctcgt gtgctgggcg ttattgcgct gaaagatatc gtcaaaggcg gtattaaaga
     3721 gcgcttcgcc cagctgcgca aatgggcatt aaaacggtga tgattaccgg cgataaccgt
     3781 ctgactgccg ccgcgatagc tgcggaagcg ggtgtcgatg attttctcgc cgaagcgaca
     3841 ccggaggcca agctggcatt gatccgtcag tatcaggcgg aaggtcgttt ggtagcgatg
     3901 accggcgaca gcaccaacga tgctacggcg ctggcgcagg cagatgtcgc ggtggcgatg
     3961 aactccggca cccaggcggc taaagaggcg ggcaatatgg tggatctcga ctctaacccg
     4021 accaagttga tcgaggtggt gcacattggc aaacagatgc tgatgacccg tggctcgctg
     4081 accaccttca gcattgccaa cgatgtggcg aaatacttcg ccattattcc ggcggcgttc
     4141 gcggcaacat atccgcagtt aaatgcgctg aacatcatgc gcctgcattc gcccgactcc
     4201 gccattctca gtgcggtcat tttcaacgcc ttgattatcg tctttttgat tcctctggcg
     4261 ttaaaaggcg tgagttataa accgcttacc gcttctgcca tgttgcgccg taacttatgg
     4321 atttacggtc tgggtgggct gctggtgccg tttattggta tcaaagtcat tgatttactg
     4381 ctaaccgttt gtagtctggt gtgaggttta ccatgagtgg attacgtccg gcattatcga
     4441 catttctctt tctgttattg attactggcg gcgtttaccc gctgctgacc accgcactgg
     4501 ggcaatggtg gtatccctgg caggccaacg gttcgttgat tcgtgaaggt gatacggtgc
     4561 gcggttcggc attaatcggg caaaatttta ccggcaacgg ctattttcat ggtcgcccgt
     4621 cggcaacagc agaaatgccc tataatccac aggcttctgg cgggagcaat ctggcagtca
     4681 gtaacccgga gctggataaa cagatagccg cacgcgttgc cgcattacgg gccgctaacc
     4741 cggacgccag cacgaacgtt ccggttgaac tggtgacggc gtcggcaagc ggactggaca
     4801 ataacatcac cccgcaagcg gcggcctggc agatcccacg catcgcaaaa gcgcgtaatc
     4861 tcagcgttga acagctcacg cagctgatcg caaaatacag ccaacaaccg ctggtgaaat
     4921 atatcggcca gccggtcgtc aacattgtcg aactcaatct ggcactggat aagcttgatg
     4981 aataacgaac ccttacgtcc cgaccccgac cgtctgctgg aacaaactgc cgcgccgcat
     5041 cgggggaagc tgaaagtttt cttcagtgcc tgtgcaggcg tcgggaagac ctgggcgatg
     5101 ctggcagaag cccagcgact gcgggcgcaa gggctggata ttgtggttgg tgtggtagaa
     5161 acccatgggc ggaaagatac cgccgccatg cttgaagggc tggctgttct gcctctgaaa
     5221 cgccaggcgt accgtgggcg gcatatcagc gagtttgatc tcgatgccgc cctcgcccgc
     5281 cgtccggcgc tgatcttaat ggacgaactg gcgcacagta atgcgccagg ttctcgtcat
     5341 cctaaacgct ggcaggatat cgaagaactg ctggaagctg gcattgatgt tttcacaacc
     5401 gtcaacgttc agcatctgga aagtctgaat gatgtggtca gcggcgtcac cggaattcag
     5461 gtacgggaaa ccgtgcccga tccttttttc gatgccgccg acgacgtggt gctggtggac
     5521 ttgcccccgg acgatctgcg ccagcggctg aaagaaggca aagtctatat tgccgggcag
     5581 gcggagcgcg ccattgaaca ttttttccgc aaaggcaatc tgatcgccct gcgcgaactg
     5641 gcactgcgcc gtactgccga tcgcgttgat gagcaaatgc gcgcctggcg ggggcatcct
     5701 ggcgaagaga aagtctggca cacgcgcgac gcgatccttt tatgcatcgg ccataacacc
     5761 ggcagcgaaa actggtccgc gcggcggcgc ggctgacgtc acggctgggg agcgtctggc
     5821 acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggagaaaaaa cgtcgggcaa
     5881 ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga gacggcaaca ctttctgatc
     5941 cagcggaaga gaaagcggta gtgcgttatg cccgtgaaca taatctcggc aagattattc
     6001 tcggtcgccc agcctcgcgc cgctggtggc gtcgggaaac atttgctgac cgactggcgc
     6061 gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga
     6121 ttaacaacgc gccggatagc cgctcattta aagacaagtg gcgggtacag attcagggat
     6181 gcgtggttgc cgccgcgtta tgcgccgtta tcaccttaat tgccatgcag tggctgatgg
     6241 cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg gcgctatttt
     6301 atggacgctg gccttcggtg gttgccaccg tcattaatgt ggtgagtttc gatctctttt
     6361 ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca atatctgctg accttcgcgg
     6421 tgatgttaac cgtcgggctg gtgatcggga accttactgc tggtgtgcgt tatcaggcgc
     6481 gggtagcccg ttaccgcgag caacgcacac ggcacttata tgaaatgtcg aaagctctgg
     6541 cggtgggccg cagtccgcag gacatcgccg ccaccagcga acaatttatt gcctccacgt
     6601 ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg taaattgcag ccgttaacac
     6661 atccgcaagg aattacgcca tgggacgatg ccatcgcgca gtggagtttt gataaaggcc
     6721 tgcctgcggg cgcgggcacc gacacgttac ccggtgtacc gtaccagatt ttgccgctaa
     6781 aaagcggcga gaaaacctac gggctggtgg tggtggagcc ggggaatctg cgccagttga
     6841 tgatcccgga acagcagcgc ctgctggaga cttttacgct gttagtcgcc aatgcccttg
     6901 agcggctgac gctaaccgcc agcgaagaac aggcgcggat ggcaagcgaa cgtgaacaga
     6961 tccgcaacgc cctgctggca gcgctttcgc atgatttacg cacgccgctt acggtgctgt
     7021 ttggtcaggc agaaatctta acgctcgatc tggcaagcga aggatcaccc cacgcccgcc
     7081 aggccagcga gattcgtcag catgtgctga acactacccg actggtgaat aatctactgg
     7141 atatggcgcg aattcagtcc ggcggcttta atttgaagaa agagtggtta acgctggaag
     7201 aagtagtcgg cagcgcgctg caaatgccgg aaccgggttt atcgtcgccc atcaatcttt
     7261 ctctgccaga accgctgacc ttaatccacg ttgacgggcc actctttgaa cgggtgctga
     7321 ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt ggtatcgatg
     7381 cccacgttga gggcgaaaat ctacaactgg atgtctggga taacggcccc ggttttccgc
     7441 caggccagga gcagacgata tttgataagt ttgctcgcgg gaataaagag tcggcggtgc
     7501 cgggtgtcgg gcttgggttg gcaatttgtc gggcgattgt ggatgtacac ggcggcacca
     7561 ttaccgcgtt caaccgaccg gaaggtggtg cctgttttcg tgttacactt ccccagcaaa
     7621 ccgctcctga acttgaagac tttcatgagg atatgtgaca aacgttctga ttgttgaaga
     7681 tgaacaggct attcgtcgct ttctgcgcac ggcgctggag ggcgacggga tgcgcgtctt
     7741 tgaggccgaa actctgcaac gcggcttgct ggaagcggca acccgtaagc cagatttgat
     7801 tattctcgat ctcggcctgc ccgatggtga tgggattgag tttatccgcg acctgcgcca
     7861 gtggagcccg gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg acaagatcgc
     7921 cgcgctggat gccggagcgg atgattatct gagtaagccg tttggtattg gcgaattgca
     7981 ggcccgtctg cgcgtcgctt tacgccgcca ctccgccact ccgccaccgc cgcgcccgat
     8041 ccgctggtga aattttccgg tgttaccgtc gatttagccg cccgcgtgat tcaccggggt
     8101 gaggaagagg tgcatctcac accaattgag ttccgcctgc tggcggtgct gctcaacaat
     8161 gccggaaaag tactcaccca gcgccagctc cttaaccagg tgtgggggcc aaacgcggtc
     8221 gaacacagtc actatttgcg tatttatatg ggacatctgc gacaaaaact ggaacaggat
     8281 ccggcccggc ctcgccactt cattactgaa accggtattg ggtatcggtt tatgctttga
     8341 attgttattc aataaattcg cttaaatagt gattaattta ttattaaacc tgcgccgaat
     8401 attattattt ggcaggttta atttcttaac gcaaatataa aacacgaaat tacaacattt
     8461 aagtaacaaa acagcatcta atatggcgat attcataatt aatgatttat tatctggggt
     8521 atgatcgttt tttgttgatc ttcttcacag attatagcca tttcatggat agaataactc
     8581 taccttcaac tgacacagca agcggtaaag gtaaatggaa aataataacc gcttaatgcc
     8641 tcatataagg cggacaaccc atatcatgaa gtttacccat cgtaatagtt ttgactttca
     8701 tttctttaat gcccgctagt cttctgacta aagggcaccc caacgtacag gtctccctga
     8761 ctttaagcat tacaggttaa tacctgtatt cctcggtgct catatactgc taaccctttt
     8821 aaactctaaa taattcgagt cgcagcactt gcaacttgag gtatgacgag tatagccagt
     8881 taccgggctg gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc
     8941 ggtggagctt tgcttttttt ccgacgtgat cgatttctcc tttgagaaat tgaggacctg
     9001 ctattacctg aaataaagag atgaataaaa tgtcagaatt aaaaattgcc gttagccgtt
     9061 cttgcccgga ttgtttttcc actcatcgtg catgcgtgaa tattgacgaa agtaattata
     9121 ttgacgttgc cgccattatt ttatcagtca atgatgttga acgtggaaaa ctcgataaaa
     9181 tagacgctac tggctatggc attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac
     9241 cagcagaata tctgccacgt atttctggtg tcttcgagca ttgcgaatca cggaaagagt
     9301 tttatggacg tcagttagaa accgctgcta gccattatga aacgcaactg cgcccgccct
     9361 tcttccgcgc actggtcgat tatgtcaatc agggcaatag cgcttttgac tgcccgggtc
     9421 atcagggagg tgagtttttc cgtcgtcatc cggcagggaa tcagttcgtg gagtacttcg
     9481 gagaaacgct gttccgttcc gacttatgca acgcagacgt agcaatgggc gatctgctga
     9541 ttcacgaagg cgcgccatgc attgcacagc aacatgcggc aaaagtgttt aatgccgata
     9601 aaacatactt cgttctaaat ggcacttcat cttctaacaa agtggtttta aacgccctgc
     9661 taacaccggg tgatctggtg ctgtttgatc gcaataacca caaatctaac caccacggag
     9721 cgttgctaca ggctggtgca acaccggttt atctggaaac ggcacgtaac ccgtatggct
     9781 ttatcggtgg cattgatgcg cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg
     9841 aagtcgcacc gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag
     9901 gcacctacga cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt
     9961 gtgactacat tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg
    10021 ctgactgttc gccgctgttg ctggatctta atgagaacga tccgggtatt ctagttacgc
    10081 aatccgtaca taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca
    10141 gccacatcaa agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga
    10201 tgcacgcctc caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa
    10261 tgcatgaagg tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg
    10321 cccgcaaact gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg
    10381 atggtaaacc ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc
    10441 agtttgtacc aggggaacac tggcattctt ttgaaggcta tgcagagaat caatactttg
    10501 tcgatccttg caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg
    10561 aagcgttcgg tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc
    10621 cggaaaaatg cgatcttaac gccatcctct tcctgctgac tccggcagaa gatatggcca
    10681 aacttcagca acttgtgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc
    10741 attagcagaa gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac
    10801 cctgcgtcag ttgtgccagg aaatgcatga tttgtatgcc cgccacaacg tgaaacaact
    10861 gcaaaaagag atgttccgca aagcgcactt cccacacgtc agcatgaatc cgcaagaagc
    10921 caactacgcc tatttacgcg gtgaagtgga gctggttcgt ctaccggatg ccgaaggtcg
    10981 tatcgctgcc gaaggggcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga
    11041 aatctggggt ggtgctgttt tgcgttactt cagcgctctg gaagaaggga tcaacctgct
    11101 gccaggtttt gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca
    11161 agtttggtgc tatgtcatca agcctcgtga tgcgcaaagc accctgttga aaggggaaaa
    11221 attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg
    11281 gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca
    11341 atttcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc
    11401 gcaaaatgcg gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct
    11461 ttcggtaaat ccggtaactt tattgcgaac tatacctacg gcgtctcact gctgattgct
    11521 aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg
    11581 tcgccagtgc agattggtct tgcgaccatc ggggtgctgt ggatttgtac cgtggctaac
    11641 tttggtggtg cgcgcattac cgggcaaatc agtagcatta ccgtgtgggg gggcattatt
    11701 ccggtggtag gcctgtgcat tatcggctgg ttctggttta gcccgacgct ttacgttgat
    11761 tcctggaatc cgcatcatgc accgttcttc agtgcagtag gttcttccat cgctatgacg
    11821 ctgtgggctt ttcttggtct ggagtctgcg tgtgcgaata ctgatgtagt ggaaaacccg
    11881 gaacgtaatg tgccaatcgc ggtactcggc ggtacgttag gtgcggcggt gatttatatc
    11941 gtctccacca acgtgattgc agggattgtg cctaatatgg agctggcaaa ttcaacggca
    12001 ccatttggcc tggccttcgc acagatgttt acaccagaag tgggtaaagt cattatggcg
    12061 ctgatggtga tgtcctgctg cggttcgctg cttggctggc agttcaccat tgcccaggtg
    12121 tttaaatctt catctgatga aggctacttc cctaaaattt tctcccgtgt aaccaaagtg
    12181 gatgcaccgg tgcagggaat gttgaccatt gtgattatcc agagcgggtt ggcgctgatg
    12241 acgattagcc cgtcgctaaa cagtcagttc aacgtgctgg ttaacctggc cgtggtgacc
    12301 aatatcattc cgtatattct gtcaatggcg gcattagtca ttattcagaa ggtcgctaat
    12361 gtgcccccct caaaagcgaa agttgcaaac tttgttgctt ttgttggcgc gatgtatagc
    12421 ttttatgcgc tgtactcatc cggggaagaa gccatgctgt acggttccat cgtgaccttc
    12481 cttggttgga cactgtatgg tctggtatca ccacgctttg aactgaaaaa taaacacggt
    12541 taaaaaagca aagtaaaaaa gggcgatctt gcgaccgctc tttttttatt aaatgtgttt
    12601 acgcgttttt cagaacttcg ctaacaatct caaccgcttc tttctcaatc tgcttgcgat
    12661 gttcttcacc gaggaagctt tcgcagtaga tcttatatgc gtcttccgtg cctgacggac
    12721 gcgcggcgaa ccagccgttg tcagtcatca ctttcagacc gccaatagaa gcaccgttgc
    12781 ccggagcagc agtcaggcgc gcggtgatcg ggtcacctgc cagggtgctg gcgctcacca
    12841 tttccggaga cagcttagac agcgccgctt tttgtgcgga agtcgcagct gcctgcaaac
    12901 ggttgtagct cggcgcacca aagcgttttg ccagttcgtt gtagtgttcc tgcgggttct
    12961 taccggtgac agcggtgatt tccgccgcca gcagacacat aatgatgccg tctttgtcgg
    13021 tggaccacgg cgtgccgtcg aaacgcagga aggaagcccc tgcgctctct tcgccgccaa
    13081 agccgaagct gccgtcgaac agaccatcaa caaaccattt gaaacctacc ggtacttcta
    13141 ccagcttacg gcccaagtca ttgaccacac ggtcgatcat cgcagaagaa accagcgttt
    13201 taccgacagc aacatctttg ccccactgcg gacgatgctg gaacaggtag ttgatcgcca
    13261 ccgccaggta gtggttcgga ttcatcaaac ctgccggagt gacgataccg tgacggtcat
    13321 aatccgggtc gttagcaaac gccagatcga acttatcacg cagtgccagc agacccgcca
    13381 tcgcacactc ggaggagcag tccatacgga tcgcaccgtc tttatcgagg tgcataaagc
    13441 ggaaggtttg atcgacctga tcgttaacga tggtcaggtt gaggttgtaa tactcgccaa
    13501 tgcgcttcca gtattcgata ccggaaccgc ccaacggatc aacgcccagc gtcaggcccg
    13561 ctttctgaat cgccgccata tcaacgatat cggccagccc ttccacgaac ggctgcacca
    13621 gatcctgctc tttcacatag ccggatgcca tcgcttcgtc gagggagata cgcttcacgc
    13681 ctttcaggcc atcggccatc agtgcgttgg ccttgtcttc caccactttg gtgacgttag
    13741 tatcagccgg gccaccattt ggcggattgt acttgatacc accatcttcc ggcgggttat
    13801 gggacggtgt aatcacgata ccgtctgcca gcgggccacc ttttttattg tgaaccagga
    13861 tggcattgga aacggcaggc gtcggagtga agccattgtt ttcctgcaca atgacatcaa
    13921 cgccgttcgc tgccagcact tccagtacgg aaatgaacgc aggctcggac agggcgtgag
    13981 tatctttacc cacatagcaa gggccagtga tgccgttttt cgcacgttct tcagcaattg
    14041 cctgagcgat cgccagaatg tgcggctcgt taaagctgtg gcgcgctgca ctgccacggt
    14101 gaccggaagt accgaatttc accgcgtgct ccgcattccc tgcttctggt ttcagtacat
    14161 aatattgcgc cgtcagttgg gcgacgttaa tcaaatcact ctgttgtgca ggttggcctg
    14221 cacgattgtg gattgccatt gctttgtcct ttgtctgcaa cgttttagat agttccgcaa
    14281 accttctcaa tcaattccgc cgggaattgc atcgactgca tgatgtgttc gatcatgctg
    14341 catttacggc cggtgttggt gttggtgatc acccaatacg gcgtgcctgg cacatgtttc
    14401 ggcttggtct gattaccatt tttcagcagc gtttgttcat ctgccgcaaa gtacacgcgt
    14461 gtacgaccgt gcaacgattc cgttgcttcg gcaaacgcct gggcgtcaag agaatatagt
    14521 gtagacaaca gcagcataaa gcgattgacc gctcgctttt gctctgcgta ttcgtccgaa
    14581 agcagaagtt cacgcattgc gcgaaccttg tctttaatcg ttttgaccgg cttcgcttcg
    14641 acgatagcag gtgacgcaac gcgaacctct ttcgtcaccg gagcagcagg ctgtgatgcg
    14701 gcggaaaatt tcaacatacg ccgtaaaatg tcggatgcgc tctcgccgat atgcttagtg
    14761 tggctggcaa tatagctgta gagttcatca tcaacttcaa tcgttttcat cttaatccag
    14821 tgcagtgtct tagctgaata caagtcgcca agagtatatg aataaatccc ggcagcggat
    14881 agcgtcaagc ctggcttgca gaaaaactcg gaataaacct tatttgttgc agccctgtgg
    14941 cacaatggtc aacataatac cctaacccga caacgcgaaa aaaagaactt tgccatgaaa
    15001 ttaaatatcc gcgcgcaaac tgcacaaaac cagcacaata attctcccat cgttcttgtc
    15061 catggtctgt ttggtagcct cgacaacctt ggcgtactgg ctcgcgatct ggtaaacgat
    15121 cacaatatca tccaggttga tatgcgtaac cacggtcttt caccgagaga gccggtaatg
    15181 aattacccgg cgatggcgca ggatcttgtt gataccctgg atgcactgca gatcgacaaa
    15241 gcaacattta tcggtcactc catgggcggt aaagcggtaa tggcacttac tgcactggcc
    15301 cccgatcgca tcgataaact ggtggcgatc gatatcgcac cggtcgacta tcacgtacgc
    15361 cgtcatgatg agattttcgc agctatcaac gcggtcagtg aatcggacgc acaaactcgc
    15421 cagcaagcag cagcaataat gcgccagcat cttaatgaag aaggggtgat tcagtttctg
    15481 ttgaaatctt ttgttgacgg ggagtggcgc tttaacgtgc cggtattgtg ggatcagtat
    15541 ccgcatattg tgggttggga gaaaatcccg gcatgggatc accctgccct gtttatccct
    15601 ggcggcaatt ctccgtatgt tagcgagcag taccgtgatg atttactggc acaatttcca
    15661 caggcacggg cgcatgtgat tgcaggcgcg ggtcactggg tccatgctga aaaaccggat
    15721 gcggtattac gcgctatccg tcgctatctc aatgattaac gcaactgatt aaaaacccag
    15781 cgtccgcgtc gctttccgcg cgtgggcgtt ggcgtgactc atcgcgctga tgtattatgg
    15841 cgcgctatcc atccgggcag aggcctggct gattgtttcc cccgaagtca ccaagatcat
    15901 ggccaaagaa caaacggacc gtacgacatt agatctgttc gcgcacgagc gtcgaccggg
    15961 acgaccgaaa actaatccgc tttcgcgcga tgaacagctg cgtattaata aacgcaacca
    16021 gctaaaacgc gacaaagtac gtggccttaa gcgtgtcgaa ctgaagctga acgcggaagc
    16081 tgtcgaggcg ctgaacgagc tggcggaatc gcgtaatatg agccgtagcg aactgatcga
    16141 agagatgtta atgcagcaac tggcggcgct gcgtagtcag ggcatcgttt aaatttccac
    16201 tttcatgtag cacagtgtgc agtcctgctc gtttgctgat tccgcaagac tgcctgttct
    16261 gctatgattg cctttatccg tgggcaattt tccaccccca tttcaataag tttcaagagg
    16321 ttatttcact catggctatc actggcatct ttttcggcag cgacaccggt aataccgaaa
    16381 atatcgcaaa aatgattcaa aaacagcttg gtaaagacgt tgccgatgtc catgacattg
    16441 caaaaagcag caaagaagat ctggaagctt acgacattct gctgctgggc atcccaacct
    16501 ggtattacgg cgaagcgcag tgtgactggg atgacttctt cccgactctc gaagagattg
    16561 atttcaatgg taaactggtt gcgctgtttg gttgtggtga ccaggaagat tacgccgaat
    16621 atttctgcga cgcattgggc accatccgcg acatcattga accgcgcggt gcaaccatcg
    16681 ttggtcactg gccaactgcg ggctatcatt tcgaagcatc aaaaggtctg gcagatgacg
    16741 accactttgt cggtctggct atcgacgaag accgtcagcc ggaactgacc gctgaacgtg
    16801 tagaaaaatg ggttaaacag atttctgaag agttgcatct cgacgaaatt cttaatgcct
    16861 gatgtgatgc ggcgtagact catgtctacg ccgtattaat agataatgcc aatcaaaata
    16921 attgctacaa atttgtaact tttgctgttg tacctataca atgtcccagt gttcaagtgg
    16981 ccttgccgtt gtaaatgtaa gctgtgccac gtttttatta acaatatttg ccagggactt
    17041 gtggttttca tttaggcgtg gcaattctat aatgatacgc attatctcaa gagcaaattc
    17101 tgtcacttct tctaatgaag tgaaccgctt agtaacagga cagattccgc atgactgata
    17161 acaataccgc cctaaagaaa gctggcctga aagtaacgct tcctcgttta aaaatcctgg
    17221 aagttcttca ggagccggac aaccatcacg tcagtgcgga agatttatac aaacgtctga
    17281 tcgatatggg tgaagaaatt ggtctggcta cggtatatcg cgtactgaac cagtttgacg
    17341 acgctggtat cgtcacccgc cacaattttg aaggcggtaa atccgtattt gaactgacac
    17401 agcaacatca ccacgatcac ctgatttgcc tcgactgcgg caaggttatc gaatttagtg
    17461 atgattccat cgaagcgcgt cagcgtgaaa ttgccgcaaa acatggcatt cgcctgacta
    17521 accacagtct ctatctttac ggtcactgtg ccgaaggcga ttgccgcgaa gatgagcacg
    17581 cgcacgaagg caaataagcc agcctgaacg agaaaagcca acctgcgggt tggctttttt
    17641 atacaaggga aataagaaaa gcgagagtta cagctctcac ttatttgtta ttactgcgta
    17701 tttcctgcca gaccttatca caatcggctt tcaccgcctg atcatttccg gtttgcgttg
    17761 cacgcagaca ctgctgataa tccagtacgc gtacactttc ctggtcggca aactgctgat
    17821 gttgtttctc tttcttcagc acgtttaaca cgctctggca ggcttcaatt ttttctggcg
    17881 aaccttgcgc ggtgttgata caggcgctgt atgcctcttt cagacggctg tcttcctgtg
    17941 gtgcggttga ttgcgcacaa gctaccagcc ccgatgccat catggcgatg agaatcagtt
    18001 ttttcattga atgcggcagg cacaaaacct gccgcgtcgg gcatcagaag atggtgaatg
    18061 gtgcgattac cataaatttc acgtcacgct catcctggaa gatgttaccg taaccgccgc
    18121 cccagcttgg gatgtcggag tggttgtcgt attcggtgaa gtgcagtttg aacatcgtgc
    18181 ctttggcgcg accgtcctga atggtgtaga ccgcatccag gctgtaggca gactcttcaa
    18241 tagtccggtt tttgtcgtag tacgcatccg gattgctctg ccaggtcgca ggtttagcat
    18301 cccatgcgta aacgtaggaa gcgccgatgg cgaagcctgg aagattccag tttttcaggt
    18361 catacatcgc actgaagaag accgcttttt cgccgttagc gttgaagtcg gaacggttat
    18421 cccaccagat atccaggcgg ccgttggagg aagcgtaggt tggggtcata cgttgcagga
    18481 agtatccctg ctgactgtca gccttaatcc aggtgccttc gaggcgcaaa tccactacgt
    18541 cagccgcccg gtaaccaaag gtcaacgctt gcagccaggc ggtgccgtca taaagatcgt
    18601 tgacgctgcg atcgtcaact ttatcgcggg taccatagaa ctggtagctg gtggttaacg
    18661 ggctaccggc gatatcaaat ttgtagctgg ctttggcaaa atattgatcg atataccctt
    18721 ccgcctgacc aaatgccgct tccagtacga agttattttt gaagtcgtat ttcgcaccga
    18781 tggagtgcag ataatcaact ttggtggttt tatcgttctg ataaaactca tccatttcca
    18841 gatgccacgg cgctttgtat tcgttggtcc acatgtagga gaaactcaag gcaccagcat
    18901 cgccgtaatc aaaattcgcc ccggcttccg ccccctgata agtacccggc ataaagctcc
    18961 agtgcggcgc taacagcgtt tgaccggttg gctgaatgta acctgccctc gcccaaaccg
    19021 gaccgtattt aaatttggcc gcagctttat acaggcttat accgcttttg tcgccggacc
    19081 agtcttcgtc ataggcttta ttactttttg aaaacgcgat ttcgttcggg tgggagctgt
    19141 cgccgttttc cgccatttca atcgccgtaa acgcagcaat atcaaggccg aacatatcag
    19201 cagcataacc ggactgaaaa tcgaggttgg cattccaggt ggagtgagaa aggttggttt
    19261 tgtatttgtc gccgtcggta acatctttac ggtcgcgttc acgctgccag taatagatac
    19321 cgccggttaa ggttgaatcg tcgatgaatc cttcggcgcg agcctccggc attgccataa
    19381 agcccgacat tgctgtaaca ccggcaatag ccagcgccag cgtactacgt ttgccactaa
    19441 acgtacgcat gggttaatcc tctttgacgt ataaattgct gcaccaaagg tgaacagcga
    19501 aaaaaaataa aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac
    19561 tatttttcgc gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca
    19621 accgcacata ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca
    19681 gaagaataaa aacgtgaaat tccagcaaga tgaaagcggt tacattttat ttaattatgt
    19741 tttgtgattt taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa
    19801 agcagaaaaa acgccgcaga gcgcggcgtt tgggattgtt tgctatttct tgccggatgc
    19861 gg
//
LOCUS       DAJTUP010000075        19809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTUP010000075 DAJTUP010000000
VERSION     DAJTUP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.075"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO468_002738"
     CDS             complement(61..819)
                     /gene="aroD"
                     /locus_tag="OO468_002738"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR6259794.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNLDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO468_002739"
     CDS             complement(850..1716)
                     /gene="ydiB"
                     /locus_tag="OO468_002739"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR6259795.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1728..3000)
                     /locus_tag="OO468_002740"
                     /pseudo
     CDS             complement(1728..3000)
                     /locus_tag="OO468_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3183..4388)
                     /locus_tag="OO468_002741"
     CDS             complement(3183..4388)
                     /locus_tag="OO468_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259796.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="OO468_002742"
     CDS             complement(4488..4844)
                     /locus_tag="OO468_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR6259797.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="OO468_002743"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO468_002743"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="OO468_002744"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="OO468_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR6259798.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="OO468_002745"
     CDS             6774..9830
                     /locus_tag="OO468_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR6259799.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="OO468_002746"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="OO468_002746"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR6259800.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="OO468_002747"
     CDS             10337..10525
                     /locus_tag="OO468_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR6259801.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="OO468_002748"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="OO468_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR6259802.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="OO468_002749"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="OO468_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR6259803.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="OO468_002750"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="OO468_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR6259804.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="OO468_002751"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="OO468_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR6259805.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="OO468_002752"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="OO468_002752"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR6259806.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="OO468_002753"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="OO468_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR6259807.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="OO468_002754"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="OO468_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR6259808.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO468_002755"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO468_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR6259809.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="OO468_002756"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="OO468_002756"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR6259810.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5172 a         4783 c         5066 g         4788 t
ORIGIN      
        1 cggttaaaaa tacttttttt atttcggcgg gggagggtat ttccgccgaa atattattgc
       61 ttatgcctga tgtaaaatag ttaataccgt gcgcaaatca tttaccgaga tttgccctgg
      121 cgcagaggct ttttttaccg caccaaaagt tgccgccgag ccaaatactt caccaaccag
      181 acgagaaatt acgccagttt ttgccatcga catcgtgatg attggacgat cggcatactg
      241 ctcctgcatc tccagggtcg cggcaagcaa cgtcagcaca tcgctggtac tttgcggcat
      301 cagcgcaatc ttaggaatat cggcgtcgaa ggactgcatt ttgcgcagac gggcaatgat
      361 ttcttcggct tccggcgttt tatggaagtc aaggttggac atgacaactt tcacatcatg
      421 cgcgtgggcg taggcgacgg tttctttgac ctgatcatcg ccggtaaata actccagatc
      481 gatcatatca accaggccgc tgtcgatggc tgcacgattg agagcaatat aagcctcggt
      541 ggaaatcgcc tgctcgccgc cttctttggc actgcggaag gtaaacagca gcggtttttc
      601 tggcatggtt tcacggagaa tttttgccgc cgccatgaca gactccacat tggagaggtc
      661 ggcaaagtgg tccacacgcc attccagaat atcaaagtcc gcttcacgat aggcgagagc
      721 ttcggatttc acgcgggcga tatctttcgc catcagcgag acgatgattt taggtgcgcc
      781 cgcaccaatg acgagatctt ttacagttac ggttttcatt ttttaccctt tctgcacgcg
      841 gtcagcctgt caggcaccga accccatgac ctgtttaaca tattccagag ggaaatcttt
      901 gccagtccat aacgtgaact gttcagcccc ttgccacaac aacatgccgt atccatcaat
      961 cgttttgcaa ccagcttgtt gtgcctgctg caataacttc gtcatatgcg ggttatacac
     1021 gcattcagtg accagcagtc ccggatgtaa cagactgata tcattaacca atgattcatt
     1081 ctcaaggggt ttcataccta cttttgtgcc attggttaaa atgtcggcgg aagccagggc
     1141 ttcagcaaag gcttgctgat cggcgagatc ggtatccgtg acgacacaat cggtgttttc
     1201 gttaacccgc tgcgcgaagg cgagggcttt atcgaagaac tcatcccgac ggttaaagag
     1261 tttaatttct tttaaacctt caattgcccc ctgcgcgcca atcgccgttg aggcacctcc
     1321 ggcccctaac agcaccatcg ttttgccttt gatatcaaaa ccgctctctt taatggcgcg
     1381 aatatggccc gtgccgtcgg tgttatagcc acgcagatag ccatcatcat taacgatggt
     1441 gttgatggca cccaccagtt tggctgctgg cgttaattca tcaacatatt cacacgccag
     1501 ttgtttgttc ggcatcgaca caccagttcc gcgcattttg agggctttta atccttcaat
     1561 tgctccggga aagctatcgt tatccacttc gaaggccata taggtaaatg gcaatcccgc
     1621 tttttctaag gctttattct gcatttcagg cgataaactg tggcggatag gataagccat
     1681 caatccaatc aattcgtatt ttgcggtaac atccataatt aactccttta acctctatgc
     1741 ttaattgtgt ttaaccgggt gctaaatttg cgttcgccaa accgcacatc attctcagga
     1801 ataataaaaa cgcggtaata gcggctaaaa acaataattg cggtaatcag ggccagcagc
     1861 gcgaaagtaa aatcgagaac aatgatatat tgcaggccga tgttcgacag ataaccggta
     1921 atcagcggaa taacaaagtt agccagtcca cccatcatca tataaatact ggtgactttg
     1981 gctttgcttt tggggaaaaa ctctgacatg accgaaacac cgagctgtaa aatgccgcca
     2041 gctgctgaga aaccgataac aaaggcgccg gcattacaca ccagtggaga agggtacagg
     2101 tagataatgg cagctgttat tgttgccagc gcagagttaa atacattagc ccagatgggc
     2161 cggaccattt ttttcagtag tgcggcaaaa ataaagacac agaccaacga gcccatactg
     2221 taataagaga tggtttttaa tgcctcagct tctgacatac cagcaaaagc catcgcatat
     2281 ttgggcatcc acaccacaat cacataaaag gtcgagaatg cggctacacc gaacagtacc
     2341 gatgaaacac cttccagcca gactaaaggt tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 cgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cctggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctg
//
LOCUS       DAJTUP010000076        19678 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTUP010000076 DAJTUP010000000
VERSION     DAJTUP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.076"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(141..1574)
                     /gene="hldE"
                     /locus_tag="OO468_002757"
     CDS             complement(141..1574)
                     /gene="hldE"
                     /locus_tag="OO468_002757"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR6259811.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1622..4462)
                     /gene="glnE"
                     /locus_tag="OO468_002758"
     CDS             complement(1622..4462)
                     /gene="glnE"
                     /locus_tag="OO468_002758"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR6259812.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4485..5786)
                     /gene="ygiF"
                     /locus_tag="OO468_002759"
     CDS             complement(4485..5786)
                     /gene="ygiF"
                     /locus_tag="OO468_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR6259813.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6028..6648
                     /locus_tag="OO468_002760"
     CDS             6028..6648
                     /locus_tag="OO468_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR6259814.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6712..7950
                     /gene="cca"
                     /locus_tag="OO468_002761"
     CDS             6712..7950
                     /gene="cca"
                     /locus_tag="OO468_002761"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR6259815.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8113..8934)
                     /gene="bacA"
                     /locus_tag="OO468_002762"
     CDS             complement(8113..8934)
                     /gene="bacA"
                     /locus_tag="OO468_002762"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR6259816.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9025..9393)
                     /gene="folB"
                     /locus_tag="OO468_002763"
     CDS             complement(9025..9393)
                     /gene="folB"
                     /locus_tag="OO468_002763"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR6259817.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9498..10115
                     /gene="plsY"
                     /locus_tag="OO468_002764"
     CDS             9498..10115
                     /gene="plsY"
                     /locus_tag="OO468_002764"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR6259818.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10128..11059)
                     /gene="ttdR"
                     /locus_tag="OO468_002765"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10128..11059)
                     /gene="ttdR"
                     /locus_tag="OO468_002765"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11266..12109
                     /gene="ttdA"
                     /locus_tag="OO468_002766"
                     /pseudo
     CDS             11266..12109
                     /gene="ttdA"
                     /locus_tag="OO468_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12106..12711
                     /gene="ttdB"
                     /locus_tag="OO468_002767"
     CDS             12106..12711
                     /gene="ttdB"
                     /locus_tag="OO468_002767"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR6259819.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12759..14222
                     /gene="ttdT"
                     /locus_tag="OO468_002768"
     CDS             12759..14222
                     /gene="ttdT"
                     /locus_tag="OO468_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR6259820.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14265..15278)
                     /gene="tsaD"
                     /locus_tag="OO468_002769"
     CDS             complement(14265..15278)
                     /gene="tsaD"
                     /locus_tag="OO468_002769"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR6259821.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15516..15731
                     /gene="rpsU"
                     /locus_tag="OO468_002770"
     CDS             15516..15731
                     /gene="rpsU"
                     /locus_tag="OO468_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR6259822.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15842..17587
                     /gene="dnaG"
                     /locus_tag="OO468_002771"
     CDS             15842..17587
                     /gene="dnaG"
                     /locus_tag="OO468_002771"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR6259823.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17782..19623
                     /gene="rpoD"
                     /locus_tag="OO468_002772"
     CDS             17782..19623
                     /gene="rpoD"
                     /locus_tag="OO468_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR6259824.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4681 a         5500 c         5027 g         4470 t
ORIGIN      
        1 ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc cttacccttg
       61 ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct acgaagcgag
      121 atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt gatgatgtta
      181 gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt ggcccagact
      241 tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag taaatctggc
      301 aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac ccagtcgacc
      361 gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg gcgagaatcc
      421 cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg gtcacccagt
      481 ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa gacgccattg
      541 gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt cagttcctct
      601 tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc cagctcgatc
      661 ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc attggcaaag
      721 aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac gccaatcacc
      781 gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat atgcagcggc
      841 gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa cagagccgag
      901 agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc ggtcttacat
      961 ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc gccgcggtag
     1021 cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt acgcgccagt
     1081 tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag caccagcgcg
     1141 ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc aacaccttcg
     1201 aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac ccgtaatttg
     1261 gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac gtcggccaga
     1321 gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag gcgtgcatta
     1381 gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg ttcttcgatg
     1441 gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt ggggccgtac
     1501 cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc aaactctggc
     1561 agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat aataccatac
     1621 ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc tctgcggtga
     1681 agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga agttcatcgc
     1741 gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata atgtcgtttt
     1801 gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc ggtttttcat
     1861 gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt ccgccttcat
     1921 cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc attttctcgc
     1981 gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc ataatctcac
     2041 gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca cgcgcacgca
     2101 ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca aatgcttctg
     2161 cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc acttcataca
     2221 aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc agacgcagat
     2281 aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt gggcaatcat
     2341 ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc agcttgccgt
     2401 agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc ttaccgtagc
     2461 gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct tctgccagcc
     2521 aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc gctgccgcaa
     2581 tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt tcttcgtcat
     2641 cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg gtcgccgtcg
     2701 gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa cgcgctagct
     2761 ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc gccgcaggga
     2821 attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc aaggcggtaa
     2881 tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc agatgtggca
     2941 tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc agctctttgc
     3001 ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga tgcgccagca
     3061 ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac tgttccgaca
     3121 gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac acccggcgca
     3181 cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca aagtccatcg
     3241 cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt tgttcgtcgt
     3301 taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact cgcaattgtt
     3361 cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt ggcagtaaag
     3421 agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc tgaacgataa
     3481 attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa ccacgtcgac
     3541 gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg ctgaaatcga
     3601 tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca tagacgcctt
     3661 cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg cgtccctgct
     3721 cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt tcgccaaacg
     3781 gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga tccagcactt
     3841 taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc cggcgtccgc
     3901 cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag gagaaattca
     3961 gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct tcgccctgcg
     4021 cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca cgcgccgcaa
     4081 caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca gtaaccagtg
     4141 ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc cgcagctcgc
     4201 gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac acgctgtaat
     4261 gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac tctggatgcg
     4321 caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc tgtgcactaa
     4381 gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag tactgctgta
     4441 acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg ttttccgctg
     4501 tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat ttcaatgcgt
     4561 tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc ttgcgcgacg
     4621 acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat caggcgtggc
     4681 aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag ttcagcggca
     4741 tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt gccctgcgct
     4801 ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa cgccagcttc
     4861 gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga agcgatggtc
     4921 gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg cacaatgcca
     4981 ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg ctctttcgcc
     5041 gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa caccagctcg
     5101 agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa aatggtggtc
     5161 ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc cgctttgctc
     5221 aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt cagcaccgcg
     5281 cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc aaattcaccc
     5341 gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat ctctactagc
     5401 catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc gagatcggca
     5461 ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag cgtcggttcg
     5521 ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct tcctgcaact
     5581 ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat atcgtgccta
     5641 cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg gtcatggtgc
     5701 tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg attaacaata
     5761 aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca cataagtgac
     5821 gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg cgccaaattg
     5881 ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga cctgacacag
     5941 aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta ctatcgttcc
     6001 actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg attaacttta
     6061 ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt ttctgacgaa
     6121 ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg cacggttaac
     6181 gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc ccaggtgaaa
     6241 gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga gccaagcctg
     6301 cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa actcaccaat
     6361 atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc gcagagcgac
     6421 agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct gattgtcgcg
     6481 cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac catcatcatg
     6541 caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg tctggtactg
     6601 ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat cgccttcttc
     6661 gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg cgtgaagatt
     6721 tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa agacagagat
     6781 tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca gcaggtaggc
     6841 cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct ggcacgtacc
     6901 gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc ggatgtcacg
     6961 ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca ggacgataac
     7021 ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct gttgcgccat
     7081 gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg ttttgctgcg
     7141 cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat gcgcgagatg
     7201 acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga gacggaaagc
     7261 gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg cggcgcactt
     7321 cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc caggtggcat
     7381 ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc gatgctgagt
     7441 ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg actgacgccg
     7501 ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt agtggaacaa
     7561 ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact ggtggctgag
     7621 tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt caaattattt
     7681 gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct gaccagcgag
     7741 gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg ccgttggttg
     7801 cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga agcgggattt
     7861 aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt agccagctgg
     7921 aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc acgaaccgtc
     7981 ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc ggtgcgaaca
     8041 atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc ctgctcccga
     8101 accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa agcgataaat
     8161 ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa tcgctatcag
     8221 cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc cggttgtcag
     8281 gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg gtaccgccag
     8341 caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc cacctgaaat
     8401 ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac agccaatcat
     8461 aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt tcggcttcag
     8521 gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca catttatcgg
     8581 gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg ccggaatcat
     8641 ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt gctgcaacgg
     8701 gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca ctactaccgc
     8761 cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc cctcaaaccc
     8821 caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa attctgtcaa
     8881 tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc tcatcaataa
     8941 acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac caggttataa
     9001 ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc cacgctcaat
     9061 gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt tgatacgcac
     9121 ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca cgcgttccac
     9181 cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt cagcgtaact
     9241 gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg ccatttcgat
     9301 atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa tagtggtgat
     9361 taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt ttggctaacc
     9421 ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga acacgaattt
     9481 tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc gcgtacctct
     9541 gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc gatccgcgaa
     9601 ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc aagggagcag
     9661 ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg ggcgcgtatg
     9721 aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt cttggacaca
     9781 tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct tttggagcaa
     9841 tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta ctgaccgtgc
     9901 tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct ccgttttatg
     9961 tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc ctgatcctgc
    10021 tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa atctggacga
    10081 aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta catgacctga
    10141 tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa ttccgcgcag
    10201 acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata ttggcgcttt
    10261 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    10321 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    10381 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    10441 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    10501 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    10561 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    10621 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    10681 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    10741 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    10801 cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg taacaacgtt
    10861 ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt gttgccagcg
    10921 tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca agcgtcgccg
    10981 ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag tctttggcta
    11041 aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag tgtatttcgc
    11101 aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg ttgattcacg
    11161 aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt agtgttagtt
    11221 tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat gagcgaaagt
    11281 aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac cgccatgatt
    11341 tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga tgccgaaacg
    11401 tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa agcgattgac
    11461 ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa agtcggttcc
    11521 cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga agaggcaacc
    11581 gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa caccggcaaa
    11641 aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa tgacgatgcg
    11701 gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc gaaagtgtta
    11761 atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat ctccaccctc
    11821 gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc ggtggaaacc
    11881 gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca tcccaatcca
    11941 aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg gattggtcca
    12001 caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc tgctggttca
    12061 tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa aaagatcctg
    12121 acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt gatctacctg
    12181 accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga actgaaacgt
    12241 ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat cgtgcgcaaa
    12301 aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg tatggaaagt
    12361 tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa aggtggtatg
    12421 gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat tttcccagca
    12481 ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca ctggacagaa
    12541 ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc gctgattgtc
    12601 tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt cgccgaacgc
    12661 cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata accctcccgg
    12721 agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc actgaatggt
    12781 ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg gttcccgcag
    12841 ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc gttggactga
    12901 tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc atcgccatac
    12961 tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt aagttcaccg
    13021 ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg ctcattttcg
    13081 ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc attgcgctga
    13141 ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg atgttctccg
    13201 agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt attatctatc
    13261 ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc agttcgcgca
    13321 gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc agcgccattt
    13381 tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc gcatctcacg
    13441 ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt ttactggttc
    13501 tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct ggtgatcagg
    13561 tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg cgtgaaaaac
    13621 ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt gattatatcg
    13681 atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc attatcagtt
    13741 gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg gcctcgctta
    13801 tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc aaactgttag
    13861 caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt gtggtgtttt
    13921 atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc gcaccgatga
    13981 tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg
    14041 gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc agtccgattt
    14101 actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg atttttgggc
    14161 tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg gtgttgcttt
    14221 aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc agccggtaac
    14281 tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc gcctgcttta
    14341 aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa ttccggacgc
    14401 gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc ccgtaacgtg
    14461 cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg atccaacgct
    14521 cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg ggcgatatca
    14581 gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa ggttttcaga
    14641 ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa aacaaagcgc
    14701 ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata atccagcccc
    14761 agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga ctcgccgagc
    14821 agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc ggacaccagc
    14881 agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa cagatgccct
    14941 tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga acgccccacg
    15001 gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc cacagcatca
    15061 atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg tacggtttta
    15121 cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc gtgcaatttc
    15181 acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg
    15241 gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac ctcgctttat
    15301 taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc ctggagaaag
    15361 cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc ggtgctttac
    15421 aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg cgttgatgcc
    15481 agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa gtacgtgaaa
    15541 acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa gcaggtgttc
    15601 tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt aagcgcgcta
    15661 aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca cgccgcactc
    15721 gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta gttgtaaggc
    15781 cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa aatcggggcc
    15841 tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca ctgacatcgt
    15901 cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc acgcgtgttg
    15961 tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac agttttacca
    16021 ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact acgacaagct
    16081 cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag tgccatttga
    16141 agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt atcagttgat
    16201 ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca cgtctgcgcg
    16261 ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg cgattggttt
    16321 tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag aaaatcgcca
    16381 gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt acgatcgttt
    16441 ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg gttttggcgg
    16501 gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag acattttcca
    16561 taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg aacccaatcg
    16621 tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg gcattaatta
    16681 cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt tgttccgcgc
    16741 gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg ccgcatggcg
    16801 agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct ttatgttttt
    16861 gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag cgtttgaagc
    16921 gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga tgccgcaagt
    16981 tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc cattgatatc
    17041 gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca acaaattagg
    17101 catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga gcggcgtttc
    17161 tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt tgctggtgca
    17221 aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa ataagctccc
    17281 tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag gtctgaccac
    17341 cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg aaaaactgtc
    17401 gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg actcactcaa
    17461 ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc gtgagcgcac
    17521 gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg agctggcgaa
    17581 aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac agccgcactg
    17641 agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt aaacaaccgt
    17701 tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc aacctcatga
    17761 aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa acttcttgtc
    17821 acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca tctgccggaa
    17881 gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga catgggcatt
    17941 caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa caccgcggac
    18001 gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga aatcgggcgc
    18061 acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact gttgacccgc
    18121 gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt tcaatgctcc
    18181 gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg tgttgaagca
    18241 gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc agaagaagat
    18301 ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct ggacgatgac
    18361 gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa cagcatcgac
    18421 ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt aacgcgtgac
    18481 accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct gaaactgtct
    18541 gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt caacagcatg
    18601 cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct ctgcgttgag
    18661 cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga aaccagcgat
    18721 acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact gcacgatgtc
    18781 tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga aaccggcctg
    18841 accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc gaaagcccgc
    18901 cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat cgccaagaaa
    18961 tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat cggcctgatg
    19021 aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta cgcaacctgg
    19081 tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat ccgtattccg
    19141 gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat gctgcaagag
    19201 atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc ggaagacaag
    19261 atccgcaaag tgctgaagat cgccaaagag ccaatctcca tggaaacgcc gatcggtgat
    19321 gatgaagatt cgcatctggg ggatttcatc gaggatacca ccctcgagct gccgctggat
    19381 tctgcgacca ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg cctgaccgcg
    19441 cgtgaagcaa aagttctgcg tatgcgtttc ggtatcgata tgaacaccga ccacacgctg
    19501 gaagaagtgg gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat cgaagcgaag
    19561 gcgctgcgca aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt cctggacgat
    19621 taatcggtag gccggatcag gcgttacgcc gcacccggca ctagtctctc tgcacaaa
//
LOCUS       DAJTUP010000077        18870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTUP010000077 DAJTUP010000000
VERSION     DAJTUP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.077"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..2085
                     /gene="pbpC"
                     /locus_tag="OO468_002773"
     CDS             <1..2085
                     /gene="pbpC"
                     /locus_tag="OO468_002773"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR6259825.1"
                     /translation="LEALINYEDRWFWKHPGVNPFSVARAAWQDLTSGRVISGGSTLT
                     MQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAPFGGTLQGIGAASW
                     AYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVLERMAAQGVWSREQ
                     VKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQRRLEELAQNWKGR
                     LPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRSPGSVLKPFVYGLA
                     LDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSLNLPAVQVLEAYGP
                     KRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAFARHGKAGKLRLQP
                     DDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTGTSYGYRDAWAIGV
                     NARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANLPEDPRPNSVTRGV
                     ICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIRFPIWLDDKGQRVA
                     ADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHDAQLPLQLTGVRDG
                     AIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHLTDKGDYQLLVMDD
                     VGQIATVKFVMQ"
     gene            2235..2666
                     /gene="ndk"
                     /locus_tag="OO468_002774"
     CDS             2235..2666
                     /gene="ndk"
                     /locus_tag="OO468_002774"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR6259826.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2816..3970
                     /gene="trmG/rlmN"
                     /locus_tag="OO468_002775"
     CDS             2816..3970
                     /gene="trmG/rlmN"
                     /locus_tag="OO468_002775"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR6259827.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4255..5268
                     /gene="rodZ"
                     /locus_tag="OO468_002776"
     CDS             4255..5268
                     /gene="rodZ"
                     /locus_tag="OO468_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR6259828.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5295..6422
                     /gene="ispG"
                     /locus_tag="OO468_002777"
                     /gene_synonym="gcpE"
     CDS             5295..6422
                     /gene="ispG"
                     /locus_tag="OO468_002777"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR6259829.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6525..7799
                     /gene="hisS"
                     /locus_tag="OO468_002778"
     CDS             6525..7799
                     /gene="hisS"
                     /locus_tag="OO468_002778"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR6259830.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7817..8437
                     /gene="yfgM"
                     /locus_tag="OO468_002779"
     CDS             7817..8437
                     /gene="yfgM"
                     /locus_tag="OO468_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR6259831.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8448..9626
                     /gene="bamB"
                     /locus_tag="OO468_002780"
     CDS             8448..9626
                     /gene="bamB"
                     /locus_tag="OO468_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR6259832.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9744..11216
                     /gene="der"
                     /locus_tag="OO468_002781"
     CDS             9744..11216
                     /gene="der"
                     /locus_tag="OO468_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR6259833.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11286..11501
                     /locus_tag="OO468_002782"
     CDS             11286..11501
                     /locus_tag="OO468_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR6259834.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11498..12868)
                     /gene="xseA"
                     /locus_tag="OO468_002783"
     CDS             complement(11498..12868)
                     /gene="xseA"
                     /locus_tag="OO468_002783"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR6259835.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13030..14496
                     /gene="guaB"
                     /locus_tag="OO468_002784"
     CDS             13030..14496
                     /gene="guaB"
                     /locus_tag="OO468_002784"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR6259836.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14565..16142
                     /gene="guaA"
                     /locus_tag="OO468_002785"
     CDS             14565..16142
                     /gene="guaA"
                     /locus_tag="OO468_002785"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR6259837.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16237..16776)
                     /locus_tag="OO468_002786"
     CDS             complement(16237..16776)
                     /locus_tag="OO468_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR6259838.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16792..17310)
                     /locus_tag="OO468_002787"
     CDS             complement(16792..17310)
                     /locus_tag="OO468_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR6259839.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17621..17812)
                     /locus_tag="OO468_002788"
     CDS             complement(17621..17812)
                     /locus_tag="OO468_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR6259840.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17830..17982
                     /locus_tag="OO468_002789"
     CDS             17830..17982
                     /locus_tag="OO468_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259841.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18164..>18870
                     /gene="pdeF"
                     /locus_tag="OO468_002790"
     CDS             18164..>18870
                     /gene="pdeF"
                     /locus_tag="OO468_002790"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR6259842.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLN"
BASE COUNT         4450 a         4592 c         5148 g         4680 t
ORIGIN      
        1 cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg ggttaatcca
       61 ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat ttccggtggc
      121 agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac atttggtggc
      181 aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa gcgtgaaatt
      241 ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat cggtgcggca
      301 agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc ggcaatgctg
      361 gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga gcgtgccgaa
      421 gccgcgcgta ataaagtgct cgaacggatg gctgcgcaag gtgtgtggtc ccgtgagcag
      481 gtaaaagagt caagggaaga acccatctgg ctggcacccc gacaaatgcc gcaactggca
      541 ccgctgtttt cgcgcatgat gctcggtaaa agcaaaagcg acaaaatcgt tactacgctg
      601 gatgccggtc ttcaacgacg tctggaagaa ctggcgcaaa actggaaagg gcggttgcca
      661 ccgcgcagct cactggcgat gatcgtggtt aatcataccg atatgcgtgt tcgcggctgg
      721 gtgggatcgg ttgatctcaa cgatgattca cgctttggtc atgttgatat ggtcaatgcg
      781 atccgatcgc caggatcggt gctcaaaccg tttgtttatg gtctggcgct ggatgaaggc
      841 ttgatccacc cggcatcact gctgcaagac gtcccccggc gcaccggtga ttatcgacca
      901 ggtaactttg atagcggttt tcatggcccg atcaacatga gcgaggcgct ggtgcgctcg
      961 ctgaacttac ctgctgtgca ggtgctggaa gcctatggac cgaaacggtt tgcggcaaag
     1021 ttacgcaatg ttggattgcc gttatatttg cccaacggcg ctgcgccgaa tctttcactc
     1081 attctcggcg gcgctggtgc aaaactggaa gatatggcgg cagcgtatac cgcgtttgct
     1141 cgccacggca aggcaggcaa attgcgctta cagcctgacg atccgctgct tgaacggcct
     1201 ttaatgtcgt cgggggcggc gtggatcatt cgccggatta tggctgatga agcgcaaccc
     1261 ttgccggata gtgccttgcc gcgcgtcgcc ccactggcat ggaaaacggg caccagctat
     1321 ggctatcgtg acgcctgggc gattggggtt aacgctcgct atgtcattgg gatctggact
     1381 ggcagacctg acggcacgcc cgttgttggt cagtttggct ttgccagtgc cgtaccattg
     1441 ttgaatcagg tcaataatat cttactgtcg cgcagtgcga atctgccgga agacccgcgc
     1501 ccgaactcag tcactcgtgg cgttatctgc tggccgggcg gacagtcttt gccggaaggt
     1561 gacggtaact gtcgccgccg cctggcaacc tggctgctgg atggcagcca gccgccaacg
     1621 ctgttactgt cagagcagga agggattaat ggcattcgct tccccatctg gctggatgac
     1681 aaagggcagc gcgtcgccgc tgactgcccg caggcgaagc aagaaatggt aaatgtctgg
     1741 ccgctaccgc tggaaccctg gctgcccgca tcagaacgtc gcgctgtgcg cttaccaccg
     1801 gcctcgacaa tttgcccgcc atacggtcac gatgcccagc tccctctgca gctgacaggc
     1861 gttcgcgatg gcgcgattat taaacgttta ccgggcgcag cggaagcgac tttgccgttg
     1921 caatcaagtg gaggggcagg tgaacgctgg tggtttctga atggcgaacc gttaactgaa
     1981 cgcgggcgca acgtgaccct gcatttgacg gataaaggtg attatcaatt gctggtgatg
     2041 gatgatgtgg ggcaaatcgc gacagtgaaa tttgtcatgc aatagtctgt tttgtccctg
     2101 attgttgcta aaactcattt tattttaaaa aaaatgttac cttcatcaat agtcaacggc
     2161 cctgttgctc attataatcc gcgccatctc gtacgctggt acagacaaca acagaacaat
     2221 ttacagaggt aaaaatggct attgaacgta ctttttccat catcaaaccg aacgcggtag
     2281 caaaaaacgt cattggtaat atctttgcgc gctttgaagc tgcagggttc aaaattgtcg
     2341 gcaccaaaat gctgcatctg accgttgaac aggcacgtgg cttttatgct gaacacgatg
     2401 gaaaaccgtt ctttgatggt ctggttgaat tcatgacctc tggcccgatc gtggtttccg
     2461 tgctggaagg tgaaaacgcc gttcagcgtc accgcgatct gctgggcgcg accaatccgg
     2521 caaacgcact ggctggtact ctgcgcgctg attacgctga cagcctgacc gaaaacggta
     2581 cccacggttc tgattccgtc gaatctgccg ctcgcgaaat cgcttatttc ttcggcgaag
     2641 gcgaagtgtg cccgcgcacc cgttaataat ttcgtaaatg ccgcgtgcaa acgtggcatc
     2701 cgtgcgccag aatttgtaca atgcagcgcc cccggacgag cagccgctca ccggggcgtt
     2761 tcttttttca accctccagg ggccataacg tgtaataacg aggccggaat agcatatgtc
     2821 tgaacaatta gtcacacctg aaaacgtcac cacgaaagat ggaaaaatca acctgctgga
     2881 tctcaaccgt cagcagatgc gggagttttt taaagattta ggtgaaaaaa ccttccgcgc
     2941 cgatcaggtg atgaagtgga tgtatcacta ttgctgcgac aactttgatg agatgaccga
     3001 catcaacaaa gtgttgcgcg gcaaactgaa agaggtggcg gaaatccgcg caccggaagt
     3061 ggttgaagaa cagcgttcat ccgacggcac cattaaatgg gcgatcgccg ttggcgatca
     3121 gcgcgtcgaa acggtgtata tcccggaaga cgaccgtgcc acgctctgcg tctcttcgca
     3181 ggtggggtgt gcgctggagt gtaaattctg ttccaccgcc cagcagggct ttaaccgcaa
     3241 cctgcgggta tcggaaatta ttggccaggt gtggcgtgcg gcgaaaatcg tcggcgcagc
     3301 aaaagtcacc ggtcagcgcc caatcactaa cgtcgtgatg atgggcatgg gcgagccgct
     3361 gctcaacctg aacaacgtcg ttccggcgat ggaaatcatg cttgatgatt tcggttttgg
     3421 cctgtctaaa cgtcgcgtga cgctttccac ttccggtgta gttccggcgc tggataaact
     3481 gggcgatatg atcgacgttg cactggcaat ttccctgcac gcgccgaacg acgaaattcg
     3541 tgacgaaatt gtgccgatca acaaaaagta caatatcgaa acgttccttg ctgcggtgcg
     3601 ccgttatctg gagaaatcca acgccaatca gggccgagtc actattgaat acgtgatgct
     3661 tgaccacgtt aacgacggca ctgaacacgc gcaccaactg gcggaactgc tgaaagatac
     3721 gccgtgtaag atcaacctga tcccgtggaa cccgttcccg gacgcgccgt atggacgcag
     3781 ttcgaacagc cgtatcgacc gtttctcaaa agtgctgatg agctacggtt tcaccactat
     3841 tgtgcgtaaa acgcgtggtg atgatatcga tgctgcctgt ggtcagttgg cgggcgatgt
     3901 tatcgaccgt acgaaacgta ctctgcgtaa acggatgcag ggtgaagcca tcgatattaa
     3961 agcggtctga tagccattac gccacggtta ctttatgtac cgtggcgtaa tcttttatga
     4021 atgaagtatt aagttgcaat tgtctggtca atattaacgg tgcgtttttg ctgactttaa
     4081 ggcagtatgt aacgatgcaa cagtaagtta gcctcagtga atgtgggctt tgtcacgagc
     4141 acacagacgg tcttatactg tatgataacg gttaacttaa cggatgtttc gcggtgtggg
     4201 tgagcatgat ggttcaccgg catctcaatt ctcatttaaa cgtacctgca gcgaatgaat
     4261 actgaagcca cgcacgacca aaatgaagca cttactaccg gcgctcgcct gcgtaatgct
     4321 cgcgaacaac taggacttag tcagcaggcc gttgccgagc gactttgcct gaaggtttcc
     4381 acggtacgcg acattgaaga agataaggca cccgccgatc ttgcttcaac attcctgcgc
     4441 ggatatatcc gctcttatgc gcgtctggta catattccag aagaagaact gctgccaggg
     4501 ctggaaaagc aggctccact tcgggctgca aaagttgcgc caatgcagag tttttccctc
     4561 ggtaaacgcc gcaaaaaacg cgacggctgg ctaatgacct tcacctggct ggtgttgttt
     4621 gtggttatcg gcctgagcgg tgcctggtgg tggcaagacc acaaagctca gcaggaagag
     4681 atcaccacta tggccgatca atcttcggcg gaactgagca gtaatagcga gcaggggcag
     4741 agtgttccgt taaatacgtc gacaactaca gacccggcta caacgtccac gccgccagcg
     4801 tctgtggata ctaccgcaac caacacgcaa acacctgccg taactgcgcc agcaccagct
     4861 gttgatccgc aacagaatgc ggttgtttcg ccttcgcagg caaatgttga taccgccgcg
     4921 accccggtac caacggcagc aacaacgcca gatggtgctg cgccgttgcc aaccgatcag
     4981 gctggcgtga ccacgccggc ggctgatccg aatgcgctgg tgatgaactt tactgccgat
     5041 tgctggctgg aggtcactga tgctaccggt aaaaaattgt ttagcgggat gcagcgtaaa
     5101 gacggtaatt tgaatttaac cggccaggca ccgtataaac tgaaaattgg tgcgccagcc
     5161 gcagtacaga tccagtatca agggaaacct gtcgatctga gtcgttttat cagaactaac
     5221 caggttgcgc gtctgaccct caatgccgaa caatcaccgg cgcagtaaca gacgggtaac
     5281 gcgggagatt tttcatgcat aaccaggctc caattcaacg tagaaaatca acacgtattt
     5341 acgttgggaa tgtgccgatt ggcgatggtg ctcccatcgc cgtacagtcc atgaccaata
     5401 cgcgtacgac agatgtcgaa gcaacggtca atcaaatcaa ggcgctggaa cgcgttggcg
     5461 ctgatatcgt ccgtgtctcc gtaccgacga tggacgcggc agaagcgttc aaactcatca
     5521 aacagcgggt taacgtgccg ctggtggctg acatccactt cgactatcgc attgcgctga
     5581 aagtagcgga atacggcgtc gattgtctgc gtattaaccc tggtaatatc ggtaatgaag
     5641 agcgtattcg catggtggtt gactgtgcgc gcgataaaaa cattccgatc cgtattggcg
     5701 ttaacgccgg atcgctggaa aaagatctgc aagaaaagta tggcgaaccg acgccgcagg
     5761 cgttgctgga atccgccatg cgtcatgttg atcatctcga tcgcctgaac ttcgatcagt
     5821 tcaaagtcag cgtgaaagcg tctgacgtct tcctcgctgt tgagtcttat cgtttgctgg
     5881 caaaacagat cgatcagccg ctgcatctgg ggatcaccga agccggtggt gcacgcagcg
     5941 gggcagtaaa atccgccatt ggtttaggtc tgctgctgtc ggaaggcatc ggcgacacgc
     6001 tgcgtgtatc gctggcggcc gatccggtcg aagagatcaa agtcggtttc gatattttga
     6061 aatcgctgcg tatccgttcg cgagggatta acttcatcgc ctgcccgacc tgttcgcgtc
     6121 aggaatttga tgttatcggt acggttaacg cgctggagca acgcctggaa gatatcatca
     6181 ctccgatgga cgtttcgatt atcggctgcg tggtgaatgg cccaggtgag gcgctggttt
     6241 ctacactcgg cgtcaccggc ggcaacaaga aaagcggcct ctatgaagat ggcgtgcgca
     6301 aagaccgtct ggacaacaac gatatgatcg accagctgga agcacgcatt cgtgcgaaag
     6361 ccagtcagct ggacgaagcg cgtcgaattg acgttcagca ggttggaaaa ataataacgt
     6421 gatgggaagc gcctcgcttc ccgtgtatga ttgaacccgc atggctcccg aaacattgag
     6481 ggaagcgttg agggttcatt tttatattca gaaagagaat aaacgtggca aaaaacattc
     6541 aagccattcg cggcatgaac gattacctgc ctggcgaaac ggccatctgg cagcgcattg
     6601 aaggcacact gaaaaacgtg ctcggcagct acggttacag tgaaatccgc ttgccgattg
     6661 tagagcagac cccgctattc aaacgtgcga ttggtgaagt caccgacgtg gttgaaaaag
     6721 agatgtacac ctttgaggat cgcaatggcg acagcctgac tctgcgccct gaagggacgg
     6781 cgggctgtgt acgcgccggc atcgagcatg gtcttctgta caatcaggaa cagcgtctgt
     6841 ggtatatcgg gccgatgttc cgtcacgagc gtccgcagaa agggcgttat cgtcagttcc
     6901 atcagttggg ctgcgaagtt ttcggtctgc aaggtccgga tatcgacgct gaactgatta
     6961 tgctcactgc ccgctggtgg cgtgcgctgg gtatcttcga acacgtaact cttgagctga
     7021 attctatcgg ttcgctggaa gcacgcgcca attaccgcga tgcgctggtg gcattccttg
     7081 agcagcataa agaaaagctg gacgaagact gcaaacgccg catgtacact aacccgctgc
     7141 gcgtgctgga ttcaaaaaat ccggaagtgc aggcgcttct caacgacgct ccggcattag
     7201 gtgattatct ggacgaggaa tctcgtgagc attttgccgg tctgtgcaaa ctgctggaga
     7261 gcgcggggat cgcttacacc gtaaaccagc gtctggtgcg tggtctggat tactataacc
     7321 gtaccgtttt cgagtgggtg actaacagtc tcggctccca gggcaccgtg tgtgcaggcg
     7381 gtcgttatga cggtcttgtg gaacaactgg gcggtcgtgc aacaccggct gtcggttttg
     7441 cgatgggcct cgaacgtctt gtattgttag tacaggccgt taatccggaa tttaaagccg
     7501 atcctgttgt cgatatatac ctggtggctt caggtgctga tacacaatct gcggctatgg
     7561 cattagctga gcgtctgcgt gatgaattac tgggcgtgaa attgatgacc aaccacggcg
     7621 gcggcaactt taagaaacag tttgcccgtg ctgataaatg gggtgcccgc gttgctgtgg
     7681 tgctgggtga gtctgaagtg gctaacggca cagcagtagt gaaggatttg cgctctggtg
     7741 agcaaacggc agttgcgcag gatagcgtag ccgcgcattt gcgcacgtta ctgggttaag
     7801 gaaggagaag gacagcgtgg aaatttacga gaacgaaaac gaccaggtag aagcggttaa
     7861 acgctttttt gctgaaaatg gcaaagcact ggctgttggg gggattttgg gcgttggcgc
     7921 actgactggc tggcgctact ggaacagcca tcaggttgat tctgcacgct ccgcttctct
     7981 tgcctatcaa aatgcggtta ccgcagtgag cgaaggcaaa ccggatagca tcccggcggc
     8041 ggaaaaattt gctgctgaaa ataaaaatac ttatggtgcg ctggcttctt tggaacttgc
     8101 gcagcaattt gttgacaaaa atgaactaga gaaagctgcc gcccagttac aacaggggct
     8161 ggcagacacg agcgatgaaa atctcaaagc cgtgataaat ctgcgtcttg ctcgcgttca
     8221 ggtacagctc aagcaggctg atgccgcgct gaaaaccctt gataccatca aaggtgaagg
     8281 gtgggctgcc attgttgccg acttgcgtgg tgaagcattg ctgagcaaag gtgataagca
     8341 aggtgcgcgt agtgcatggg aagcaggcgt gaaaagcgat gttactccgg cactgagcga
     8401 aatgatgcag atgaaaatta ataatttgtc catctgagag ggacccgatg caattgcgta
     8461 aattactgct gccaggactg ctttccgtta cccttttaag cggctgttcg ctgtttaaca
     8521 gcgaagaaga tgtggtaaag atgtccccat tgccaaccgt tgaaaaccag tttacgccga
     8581 ccacggcgtg gagcacttcc gttggtagcg gcattggcaa cttctattcc aatcttcatc
     8641 cggcactggc ggacaacgtt gtctatgcag cggaccgcgc tggtttggta aaagcgctga
     8701 atgcggatga tggcaaagaa atctggtctg tcagcctggc cgagaaagat ggctggttct
     8761 ctaaagagcc tgcattactt tctggcggtg tgaccgtgtc tggtgggcat gtctacattg
     8821 gcagcgaaaa ggcgcaggtt tacgcgctga ataccagcga tggtactgtg gcatggcaaa
     8881 ctaaagtcgc gggtgaagca ctttcgcgcc cggtggtcag cgacggtctg gtgttaatcc
     8941 acaccagtaa cggtcagtta caagcgctga acgaagctga cggcgctgtc aaatggacag
     9001 ttaacctcga tatgccttcg ctctctttgc gtggcgagtc tgcgccggca acggcttttg
     9061 gtgcggccgt cgtgggtggc gataatggtc gcgtcagcgc agtgctgatg gaacagggcc
     9121 agatgatttg gcagcagcgt atttcccagg cgaccggttc taccgaaatt gaccgtctga
     9181 gcgatgttga cacgactccc gtcgttgtta acggcgttgt tttcgcgctg gcctataatg
     9241 gtaacctgac ggcgcttgat ctgcgcagtg gtcagattat gtggaaacgc gaactgggtt
     9301 cggtgaatga tttcatcgtc gatggcaatc gcatctatct ggtcgatcaa aatgaccggg
     9361 tgatggcgtt gaccattgat ggcggcgtta cgctgtgggc acaaagcgat ctgctgcatc
     9421 gcctgctgac ttctccggtg ctgtataatg gcaacctggt ggtcggtgac agtgaaggtt
     9481 atctgcactg gattaacgtc gaagatggtc gtttcgttgc ccagcaaaaa gttgatagtt
     9541 ccggtttcca gactgaaccg gttgccgctg acggcaaact gctgatccag gcaaaagacg
     9601 gaaccgtgta ctctattaca cgttaatcgt ctctgtcgtt cactttgaaa acggctcctg
     9661 gacaggggcc gttttcctgt ttttaacaac gacgcgaata tagcgtgcgt tgtctgatga
     9721 tttataaaaa tgaggcttta aacatggtac ctgtggtcgc gcttgtcggg cgccctaacg
     9781 taggaaaatc cacgttattt aaccgtctaa ctcgcacccg agatgcgctg gttgcggatt
     9841 tcccgggtct gactcgtgac cgtaagtacg gtcgtgcgga aattgaaggc cgtgagttta
     9901 tctgtattga taccggcggg attgatggca cagaagacgg tgtagaaacc cgcatggcgg
     9961 aacagtcgct gctggcgatt gaagaagcgg acgtcgtact gtttatggtg gatgcgcgcg
    10021 cgggcctgat gccggcagat gaagcgattg ccaaacatct gcgctcccgt gaaaaaccga
    10081 ccttcctggt ggtaaacaaa actgacggtc tggatcccga tcaggcagtg gttgatttct
    10141 actcgcttgg tttaggtgaa atctacccga tcgccgcgtc tcacggtcgt ggcgtattaa
    10201 gtctgctgga gcatgtgctg ctgccgtgga tggaagatct cgcaccgcaa gaggaagtcg
    10261 acgaagacgc tgaatactgg gcgcaatttg aagcggaaga gaacggcgaa gaagaagagg
    10321 aagacgactt cgacccgcaa agtctgccga tcaaactggc gattgtgggt cgtccgaacg
    10381 taggtaagtc tacactcact aaccgtattc ttggagaaga gcgcgttgtt gtttacgaca
    10441 tgcctggcac gacgcgtgac agcatctaca tcccaatgga acgcgatgga cgtgagtatg
    10501 tgctcattga caccgctggc gtacgtaaac gcggcaaaat caccgatgct gtagagaaat
    10561 tctccgtaat caaaacgttg caggccattg aagacgccaa cgtggtgatg ttagtgattg
    10621 atgcgcgcga aggtatttcc gatcaggatc tctcgctgct gggctttatt ctcaatagtg
    10681 ggcgctcact tgtcattgtg gtgaataagt gggatggcct gagccaggaa gtgaaagagc
    10741 aggtgaaaga aacgctggac ttccgtctgg gctttatcga ttttgctcgt gtgcacttta
    10801 tctctgcctt gcacggcagt ggtgttggta acttgtttga atcagtacgt gaagcgtatg
    10861 acagctccac ccgtcgtgtg gggacctcta tgctgacgcg catcatgacg atggctgttg
    10921 aagatcacca accgccgctg gtacgcggtc gtcgtgtgaa gctgaaatat gcccacgctg
    10981 gtggttataa cccgccgatt gtggtgattc acggtaatca ggtgaaagac ctgcctgatt
    11041 cctacaagcg ctacttgatg aactacttcc gcaaatcgct ggacgtaatg ggatcgccga
    11101 ttcgtattca gttcaaagaa ggggaaaacc cgtatgcgaa caagcgtaac accctgacgc
    11161 caacccagat gcgtaaacgt aagcgtctga tgaagcacat caagaaaaat aaataattat
    11221 ccgtaagtat tttatctgcc attccctcta tgaatgtaga gggaatggct accggaggaa
    11281 ttcatatgga actgcattgt ccaaaatgtc agcatgtgct tgatcaggat aacggccatg
    11341 cgcgttgccc atcctgcggg gaatttattg aaatgaaagc tctttgtcca gactgccatc
    11401 aaccgttgca ggtgttaaaa gcctgcggtg cagtagatta cttctgtcag cacgggcatg
    11461 gcttgatatc gaaaaaacgc gttgaattcg tgctggctta atgaaccttt ttacgcgatt
    11521 tttttaccgg ctggatgttt tttacttcac tttctatcca gccgtcttcc agacgtgtgg
    11581 ttagcatttc acccgtttta acttgcttca cttttttcag tacattgccg tcagtagcag
    11641 tagtaacgct gtatccacgc gccagcgttg acagtgggct tacggcttcg aggtgcgtta
    11701 ctgcattacc gaaacgttca cgcgtggcgc taagctgtgc gcgcaggatt tctgctaaac
    11761 gatattccag ttgctgaatg cgcgtttgcg tgcgatgaat cttcggctgt ggattttgct
    11821 gattcagccg ctgtgttaat cgctgctgct gttgcccggc acgcttaagc tgattttcca
    11881 gcgcaaagct catccgtttt tgcaggcgtt caagcatggt ttgctggcgt gccagccgga
    11941 gctgcggatg ctgctgctgc aagcgatgat gaatctgcgt aaagcgacgt gtacggttgg
    12001 cgagataata atccatcgcc atctccagcc gttgacgggc cgattgcacc tggcgcagta
    12061 actcttgctg attacggctc actacttcag cggcggcaga cggcgttggc gcacgcagat
    12121 cggcaacaaa atcggcaatg gtcacatccg tctcatgccc gacggcgctg acgaccggaa
    12181 tgcggctggc aaaaatcgcc cgcgctacgc gttcgtcgtt aaaactccat aaatcttcca
    12241 gcgaaccgcc gccgcgccca acgatcaaca cgtcgcactc attgcgctga ttcgccagtt
    12301 caatggcgcg aacaatttgc cccggcgcgt catcgccctg aacggaggtg gggtagatga
    12361 tcaccggtag agaaggatcg cgacgtttta acacatgcaa aatatcatgt agcgcagcac
    12421 cggtttttga ggtgatcaca ccaacgcaat gcgcagggga gggaagtggt tttttgtatt
    12481 gctgatcgaa caaacattca gcctgcaact tcgctttgag ctgttcgtac ttctgttgca
    12541 gcagcccttc accggccggc tgcatactct caacgattat ctggtagtcg ccgcgcggct
    12601 cgtagagcgt aatattggcg cgaactaaaa cttgttgccc atgctgcgga cggaaggtga
    12661 cccggcggtt gctgttgcgg aacatcgcgc agcgtacctg ggcggtgtcg tctttgagtg
    12721 taaagtacca gtgaccggaa gccggttgtg tgaaattaga gatttcgccg ctgatccaaa
    12781 cctgtcccat ctcatgctca agcagcagac gaaccgtttg attcaggcga ctaacggtaa
    12841 aaattgcagg ggattgagaa ggtaacatgt gagcgagatc aaattctaaa tcagcaggtt
    12901 attcagtcta tagtaacccg cccttcgggg atagcaagca ttttttgcaa aaaggggtag
    12961 atgcaatcgg ttacgctctg tataatgccg cggcaatatt tattaaccac tctggtcgag
    13021 atattgccca tgctacgtat cgctaaagaa gctctgacgt ttgacgacgt tctcctcgtt
    13081 cctgctcatt ctaccgttct gccgaatact gctgacctca gcacccagct gacgaaaact
    13141 attcgtctga atatccctat gctttccgca gcaatggata ccgtaacgga agcgcgcctg
    13201 gctattgctc tggctcagga aggcggtatc ggctttatcc acaaaaacat gtccattgaa
    13261 cgccaggcag aagaagttcg ccgtgtgaaa aaacacgaat ctggtgtggt gactgatccg
    13321 cagactgttc tgccaaccac gacgctgcgc gaagtgaaag aactgaccga gcgtaacggt
    13381 tttgcgggct atccggtcgt taccgaagaa aacgaactgg tgggtattat caccggtcgt
    13441 gacgtgcgtt ttgttaccga cctgaaccag ccggttagcg tttacatgac gccgaaagag
    13501 cgtctggtca ccgtgcgtga aggtgaagcc cgtgaagtgg tgctggcaaa aatgcacgaa
    13561 aaacgcgttg aaaaagcgct ggtggttgat gacgaattcc acctgatcgg catgatcacc
    13621 gtgaaagact tccagaaagc ggaacgtaaa ccgaacgctt gtaaagacga gcaaggccgt
    13681 ctgcgtgttg gtgctgcagt tggcgcaggt gcgggtaacg aagagcgtgt tgacgcgctg
    13741 gttgccgctg gcgttgacgt tctgctgatc gactcctccc acggtcactc agaaggcgtt
    13801 ttgcagcgta tccgtgaaac ccgtgctaaa tatccggatc tgcaaatcat cggcggcaac
    13861 gtggcaacag ctgcaggtgc acgcgctttg gcagaagctg gttgcagcgc ggttaaagtc
    13921 ggtatcggcc ctggttctat ctgtacgact cgtatcgtta ctggcgtagg tgttccgcag
    13981 atcactgccg ttgctgacgc agttgaagcc ctggaaggca ccggtattcc ggttatcgca
    14041 gatggtggta ttcgcttctc cggcgacatc gccaaagcta tcgccgctgg cgcaagcgcg
    14101 gtgatggtag gttccatgct ggcgggtact gaagaatctc cgggtgaaat cgaactctac
    14161 cagggccgtt cttataaatc ttaccgtggt atgggttccc tgggcgcgat gtccaaaggt
    14221 tcctctgacc gttatttcca gagcgataac gctgccgaca aactggtgcc ggaaggtatc
    14281 gaaggtcgcg tagcttataa aggtcgcctg aaagagatca ttcaccagca gatgggcggc
    14341 ctgcgctcct gtatgggtct gaccggctgt ggtactatcg acgaactgcg tactaaagcg
    14401 gagtttgtac gtatcagcgg tgcgggcatt caggaaagcc acgttcacga cgtgaccatt
    14461 actaaagagt ccccgaacta ccgtctgggc tcctgattct cttcgcccga tttcatgtcg
    14521 ggcgatttat attatctgtt tcacttgcct cggaataagc gtcaatgacg gaaaacattc
    14581 ataagcatcg catcctcatt ctggacttcg gttctcagta cactcaactg gttgcgcgcc
    14641 gcgtgcgtga gctgggtgtt tactgcgaac tgtgggcgtg ggatgtgaca gaagcacaaa
    14701 ttcgtgactt caatccaagc ggaattattc tttccggcgg cccggaaagt actactgaag
    14761 aaaacagtcc gcgtgcgccg cagtatgtct ttgaagcagg cgtaccgata ttcggcgttt
    14821 gctatggcat gcagaccatg gcaatgcagt tgggcggtca cgttgaagcc tctaacgaac
    14881 gtgaatttgg ctacgcgcag gtagaagtcg taaacgacag cgcactggtt cgcggtatcg
    14941 aagatgcgct gaccgcagac ggtaaaccgc tgctcgatgt ctggatgagc cacggcgata
    15001 aagttaccgc tatcccgtcc gacttcgtca ccgtagccag caccgaaagc tgcccgtttg
    15061 ccattatggc taacgaagaa aaacgcttct atggcgtaca gttccacccg gaagtgactc
    15121 acacccgcca gggtatgcgc atgctggagc gttttgtgcg tgatatctgc cagtgtgaag
    15181 ccctgtggac gccagcgaaa attatcgacg atgctgtagc tcgcatccgc gagcaggtag
    15241 gcgacgataa agtcatcctc ggcctctctg gtggtgtgga ttcctccgta accgcaatgc
    15301 tgctgcaccg tgctatcggt aaaaacctga cttgcgtatt cgtcgacaac ggcctgctgc
    15361 gtctcaacga agcagagcag gttctggata tgtttggcga tcactttggt cttaacattg
    15421 ttcacgtacc ggcagaagat cgcttcctgt cagcgctggc tggcgaaaac gatccggaag
    15481 caaaacgtaa aatcatcggg cgcgttttcg ttgaagtatt cgatgaagaa gcgctgaaac
    15541 tggaagacgt gaagtggctg gcgcagggca ccatctaccc tgacgttatc gaatctgcgg
    15601 cttctgcaac cggtaaagca cacgtcatca aatctcacca caacgtgggc ggcctgccga
    15661 aagagatgaa gatgggcctg gttgaaccgc tgaaagagct gttcaaagac gaagtgcgta
    15721 agattggtct ggagctgggc ctgccgtacg acatgctgta ccgtcatccg ttcccgggac
    15781 caggccttgg cgttcgtgtt ctgggtgaag tgaagaaaga gtactgtgac ctgctgcgcc
    15841 gtgctgacgc catcttcatt gaagaactgc gtaaagcgga cctgtacgac aaagtcagcc
    15901 aggcgttcac cgtcttcctg ccagtacgtt ccgttggcgt aatgggcgat ggtcgtaagt
    15961 atgactgggt tgtctctctg cgtgctgtcg aaaccatcga ctttatgacc gcacactggg
    16021 cgcatctgcc gtacgatttc ctcggtcgcg tttccaaccg cattatcaat gaagtgaacg
    16081 gtatttcccg cgtggtgtat gacatcagcg gcaagccgcc agctaccatt gagtgggaat
    16141 gattttgatc cctgcactat gaatgaacaa aaccctccgt tactacagag ggttttttat
    16201 cttcaagaat cataggcttg aagttactaa catcgattaa ttaaaccagc tgtccgattt
    16261 gttctcttct gctttgccca cgcttttcat cagatcgcga ccgccttcag tcatatttct
    16321 gtttgcgtca gcttcagatt gcaccacatc ggtttgcgca gctttgtgct tcagttcctg
    16381 atcgataaat tcgttttcgc gcttaacgcg ggcttcttct ttcgccagcg ccagtttttg
    16441 tttctgaatc tctaagctgc gtagctcatc ttcataactt tgatcgcgtt ttttgtccgc
    16501 agtggcttcg gcgtccagtt tatcctgacg agctttctta tttgccgctg ccgttgccgc
    16561 tcttttgttc gcggcggcct gggcatttgc gcgacgttgc ttctcttgct ggatttccct
    16621 gttgcgctcc gcgacccatt cgtcatgctg cctttgctct tcatttttac cttgctgttc
    16681 cgcttctgcg acagcagaga gttgatcctg caatgatgag gctatagccg gatagcttaa
    16741 ggaggctaag atggcgcaaa gaaaaacttt cttcatgact cctcctgatt attagctctt
    16801 ttcaggacat ttggtatttg gctgaatacg cgtttcgtta tacgttgtgg taataacaac
    16861 ggctaaacct gtcgtaaact ggcactcttt accctcctgg gtggaggtat acactttggt
    16921 gccttcctta tatgttaaag aaacaccttc cactaaggtt ttatcattca ccatagaacc
    16981 cgctgccgcg cctacagctc cgccgccaac tgcccctgcc gtcgttccgg aattgctgcc
    17041 agaaccgacg ttgtggccga taacaccgcc agcgactgcg ccaataagcg cgccgaaggc
    17101 ttgtgcgttc cgtttatttt gggagttgtc tacggcaact tttgcgggaa gaatggaaat
    17161 aatattaacg gttttagttt cttgtttggt attcagttga tcggtttgat aaacatcggc
    17221 ggcatgatcg tcagcatttg actggcatcc tgccagagtg aatgacgcta acattgccac
    17281 aggcagaaga cattttttaa atttcatcac tattccttgt tatacacatt acgaaaaata
    17341 agacatttaa gatcatcaaa agaggaaagt aaatgtattg cgtataaagg gatagattaa
    17401 atttattaaa attcacgcag ggaataattt ataaaaatat atcattacaa catatatatt
    17461 aattttatat ttatttcgca gccaaacagg ataaacatca gtcaggacaa agacgtaaaa
    17521 tttaagatga aagaatatct gccgctttga taatcccaaa gagcaaaagc atccgacagc
    17581 gaagtgccgt tatgttctgt gaaagtggag aaggtaaccg ctaacgttgt accggtgatt
    17641 cgacggagag tgtggattgc tgagcttctt ttttgctctg atggtgctgc caggcaccga
    17701 cggacgagta gataaaacgg ccaaagaaga agataaagct gatgagcagt acgatacggg
    17761 tcatgcgact gttaaatcgg tgtcgttttc gcatactagt tgcctgactc acaaaaggtt
    17821 ccttgaagta tgtcccacgc tgtggacggt acttacatta aggcacaaca ggacaaaatg
    17881 gtcaattctt cgttatgtaa aaaagcgtca gtggatacat attttaatgt tatggaagtt
    17941 aatttaatta tttacaatga tgatgtaata atgatgaatt aacatgataa acgtaataat
    18001 tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata tgacacttag
    18061 aatcaatgct ctctatagtg atgaataatc atcattcgaa gtcaggtggg atgcctgtct
    18121 gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac tgaatgcaac
    18181 ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcctgcctt ctttaatctt
    18241 gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg tttttctctt
    18301 ttatctgcct ttggcactga tgatcagcat gatgatgttt ttcagctggg cggcattgcc
    18361 agggatcacc ttagggattt ttgtccgcaa atatgcagag ctgggttttt acgaaacgct
    18421 ctcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt acagggtctt
    18481 tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc agcgtatatt
    18541 ctggcagata gtgtttcctg caacgctgtt tctgatactt ttccagtttg ctgcatttgt
    18601 aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc tcgggacttt
    18661 aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt gctacttcat
    18721 cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat taaaacagca
    18781 ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat taggtgcttt
    18841 attgttgctg ttatgcatgc cgttaaatga
//
LOCUS       DAJTUP010000078        18625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTUP010000078 DAJTUP010000000
VERSION     DAJTUP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.078"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(139..3654)
                     /locus_tag="OO468_002791"
     CDS             complement(139..3654)
                     /locus_tag="OO468_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6259843.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO468_002792"
     CDS             complement(4107..5369)
                     /locus_tag="OO468_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6259844.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO468_002793"
     tRNA            complement(5567..5642)
                     /locus_tag="OO468_002793"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO468_002794"
     CDS             complement(5748..6455)
                     /locus_tag="OO468_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR6259845.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO468_002795"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO468_002795"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR6259846.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO468_002796"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO468_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR6259847.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO468_002797"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO468_002797"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR6259848.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO468_002798"
     CDS             complement(11639..11914)
                     /locus_tag="OO468_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR6259849.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO468_002799"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO468_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR6259850.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO468_002800"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO468_002800"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR6259851.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO468_002801"
     CDS             13874..14200
                     /locus_tag="OO468_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR6259852.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO468_002802"
     CDS             14384..15103
                     /locus_tag="OO468_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR6259853.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO468_002803"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO468_002803"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR6259854.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO468_002804"
     CDS             16442..17404
                     /locus_tag="OO468_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR6259855.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18625)
                     /locus_tag="OO468_002805"
     CDS             complement(17505..>18625)
                     /locus_tag="OO468_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HCR6259856.1"
                     /translation="LAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNVLA
                     NMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVLIV
                     FSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAFLD
                     ALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILESVV
                     TTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIFMD
                     MTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGVKI
                     QHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4831 a         4543 c         4606 g         4645 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caaca
//
LOCUS       DAJTUP010000079        18606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTUP010000079 DAJTUP010000000
VERSION     DAJTUP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.079"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            88..795
                     /locus_tag="OO468_002806"
     CDS             88..795
                     /locus_tag="OO468_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6259857.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            792..2219
                     /locus_tag="OO468_002807"
     CDS             792..2219
                     /locus_tag="OO468_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR6259858.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2229..2783)
                     /locus_tag="OO468_002808"
     CDS             complement(2229..2783)
                     /locus_tag="OO468_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR6259859.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            2885..3592
                     /locus_tag="OO468_002809"
     CDS             2885..3592
                     /locus_tag="OO468_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR6259860.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            3589..5040
                     /gene="djlC"
                     /locus_tag="OO468_002810"
     CDS             3589..5040
                     /gene="djlC"
                     /locus_tag="OO468_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR6259861.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(5100..6770)
                     /gene="hscC"
                     /locus_tag="OO468_002811"
                     /pseudo
     CDS             complement(5100..6770)
                     /gene="hscC"
                     /locus_tag="OO468_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(6854..7789)
                     /gene="rihA"
                     /locus_tag="OO468_002812"
     CDS             complement(6854..7789)
                     /gene="rihA"
                     /locus_tag="OO468_002812"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR6259862.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            complement(7907..8632)
                     /gene="gltL"
                     /locus_tag="OO468_002813"
     CDS             complement(7907..8632)
                     /gene="gltL"
                     /locus_tag="OO468_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR6259863.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(8632..9306)
                     /gene="gltK"
                     /locus_tag="OO468_002814"
     CDS             complement(8632..9306)
                     /gene="gltK"
                     /locus_tag="OO468_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR6259864.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(9306..10046)
                     /gene="gltJ"
                     /locus_tag="OO468_002815"
     CDS             complement(9306..10046)
                     /gene="gltJ"
                     /locus_tag="OO468_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR6259865.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            complement(10216..11124)
                     /gene="gltI"
                     /locus_tag="OO468_002816"
     CDS             complement(10216..11124)
                     /gene="gltI"
                     /locus_tag="OO468_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR6259866.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            complement(11521..13059)
                     /gene="lnt"
                     /locus_tag="OO468_002817"
     CDS             complement(11521..13059)
                     /gene="lnt"
                     /locus_tag="OO468_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR6259867.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            complement(13084..13962)
                     /gene="corC"
                     /locus_tag="OO468_002818"
     CDS             complement(13084..13962)
                     /gene="corC"
                     /locus_tag="OO468_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR6259868.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(14052..14519)
                     /gene="ybeY"
                     /locus_tag="OO468_002819"
     CDS             complement(14052..14519)
                     /gene="ybeY"
                     /locus_tag="OO468_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR6259869.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(14516..15556)
                     /locus_tag="OO468_002820"
     CDS             complement(14516..15556)
                     /locus_tag="OO468_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR6259870.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(15709..17133)
                     /gene="miaB"
                     /locus_tag="OO468_002821"
     CDS             complement(15709..17133)
                     /gene="miaB"
                     /locus_tag="OO468_002821"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR6259871.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            17279..18454
                     /gene="ubiF"
                     /locus_tag="OO468_002822"
     CDS             17279..18454
                     /gene="ubiF"
                     /locus_tag="OO468_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR6259872.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         4567 a         4644 c         4756 g         4639 t
ORIGIN      
        1 cgtattatgt tgcgtcagaa aagtataaat aactgagcct cgaaatgtag tttacattca
       61 ttaaatgaat gtttaaggat aaattatatg gacatggggt cgcaaaaaat attgtttgca
      121 ctttccactc cgatggaaat acgcaatgaa tgctgtttac cttcgcattc atcccccaaa
      181 atgtatttag gtacatgctt ttttgacctt tcttcctcat ggggaattga tgaccgtgat
      241 gacctgctgc gcataattca ccgaatgatc gagaacggtc atgccgctcg gttggcaggg
      301 ttttatcacc gctggtttcg ttattctcca tgtgaatggc gtgactatct tgctgaatta
      361 aatgagcaag gtcaggctta tgcgcaattt gtcgccagca cagccgaatg ctgtggtgag
      421 ggaggaatta aagcatggga ttatgtccgg atgggttttt taagtcgaat gggcgtactt
      481 aataactggt tgagtgaaga agaaagtcta tggatccagt cgcgtattca tctacgggct
      541 ctacgttatt atagtaactg gcggcaatac tttgccggtt atacctttgg tcggcaatat
      601 tggcagtctc ccgaagatga tcatctgcct ttattacgcg aattcttagc gcgtaaagaa
      661 tacgacgatt ccggcaatga tatgttttat caattatttg ccagtgatga tgcgtattac
      721 cctaccttgt cctggcaacc actggctgac taccccacat gcccggaaac gcttaaggat
      781 atgagcgact tatgaaaaat tgctggaaga tcctcgatat agaggaaacg actgacgtcg
      841 atattatccg ccgcgcttat ctggcgctgt taccgtcctt tcatccagaa accgatccgc
      901 agggttttaa acaacttcgt caggcgtatg aggaggcgct acggattgcg cagtcgcctg
      961 ctaaatctgt ttggcaacca gaagaatatg aggtagcaga acatgaaatt ctgctcgcct
     1021 ttcgcgcgtt acttgcctct gatagcgaac gttttctgcc ctccgcctgg cagcgattca
     1081 ttcagcaatt aaattattgc tcgatggaag agattgatga attacgctgg tcgctgtgca
     1141 caatcgccat gaacactgcc catttatcct tcgagtgcgt ggtgttatta gcagaaagat
     1201 tgcggtggtt gcaggaggaa aacgtcgggg aaatagacga agaagaactg gaatcctttt
     1261 tatatgccat tgcgaagggg aatgttttta acttccagac cattctgcat ctgccagttg
     1321 ccgtgcaaaa tgacaccatc gatttttacc aaatgttcgc tcggatttgg tcatcgcatc
     1381 cagaatggct gacattgtat ttagcgcaac atcgcgcagt gattatcccc gatgatgcaa
     1441 aactgcacag aaatttactc cgctggtata gcgcaagccg cctgggaatc cccgaactcc
     1501 tggattacgc ccggtcgtgg cgggaagctg aacctgataa tgaagatgcg cgttattatg
     1561 aatacgcaca acgcgtctat tgtggagaag gcgaaagcct gctggcagaa ctttgttact
     1621 actggcgcga gtatccctcc acccaggcgg atgctttaat gttgcaatgg tgccgtcagc
     1681 atcgggtcta ttattaccca ttagtggtga tgatgattga agcgcgtgat ctggttaacg
     1741 atcagggaaa accgctactt tatgtccccg gcgacagcgc ccgtacgcgt tttcatttat
     1801 acgaaatact cagcgatgaa aaactctctg cgctggggcg ttcactggtc gagatggttt
     1861 tgcacaaagg acgtaagccg cggatctcac tcacgcgtga tacagaacat cccttatggc
     1921 cattatattt agttgccaaa caattagtgc aggccagcca accgacagaa gaatcattaa
     1981 tgccgattgt gagccgcctt gatgcagaag atcgttgtcc actggaagca ttaattattc
     2041 gtcgattatt aattcaggcg gcgaatttta ccggtcagga aaccgtcgag ccggagccgc
     2101 aaccgcagcc aatgcccgtt gatgatggcg ggctgggctg tctgggcgtc atcaaaatta
     2161 ttttctacat ttttatcttt gccggtttga tagggaaaat actccatctc ttcgggtgac
     2221 agtcgacgtt aatgtgcggg aagtccggca agtaatcttt ccgcattttt gtaatgttgt
     2281 ttggcggcca gttttaacca tttttgagat tcataatggt taacattaat accagtgcca
     2341 tttctgtaga tcaaacccaa agtatattga gcatgagcat acccttgttc agctgcttcc
     2401 ttataccaga gaattgcttt acgggtatcg ggtttgactc ccaggccttt caggtagatc
     2461 caccccagtt tgctttgtgc gcgcggattt tcctgttctg ctgattgctc ataccagtat
     2521 ctcgcctgtg tataatccag tggtttaccg tcgctaccat gaagataaaa ttcgccaagg
     2581 cgatattgcg atgttgagtg accatgaatc gcggaaagct ccgtggctgc cgtggttaac
     2641 ggtagactgc ctggtgatct taaataatca aaggcataat tcaccatggc tttatcacgc
     2701 aacatcgttt tcttgggttg gctaatacag gtaattgctg caactataaa aattgcgaat
     2761 atatacatta attttttatt catgtgtgaa tgcttcgatt ttctttttat tttgaaagcg
     2821 tcaagtttat actcatcaat aataaaaaag tagtcagtta atgcacttaa gggattatta
     2881 aaaaatgaat aaggaagaac aatatcttct ttttgcgctt tctgcgccca tggaaatact
     2941 taatcagagt tgcaaacccg cgcatgactc gccaaaaatg tataccggga taaaagaatt
     3001 cgatctctca tcatcctggg gaataaataa tcgtgatgat ttaattcaga ctatttatca
     3061 gatgactgat gatggtcatg ccaatgattt agctggctta tatctgacat ggcaacgctc
     3121 ttcccctgaa gagtggaaag cattaattgc tggtggctcg gagagagggt tgatttatac
     3181 ccaattcgtt gcccaaaccg ctatgtgctg tggagaaggg ggaatcaaag cctgggatta
     3241 tgttcgaatg ggatttctgt cacgggccgg tgtgctcaac aactggctga cggaagaaga
     3301 gagtttatgg ctgcaatcgc gcgtctatgc acgagctcac cattactatc acagctggat
     3361 gcactatttt tctgcctatt cactagggcg tctctactgg caatcttctc agtgcgaaga
     3421 caacgcttcg ctacgcgagg cgctaaccct gtacaaatac gatagcgccg ggagtcggat
     3481 gttcgaagag ctggcagcgg gaagcgaccg tttctatgcc acgcttccct ggcagccatt
     3541 aactgtccag cctgaatgcc cggtaacgct taaggatgtg agcgacctat gaaaacatgt
     3601 tggcaaatcc tcgaaattga aagcacgacg caaatagaca ttatccgcca ggcttatctt
     3661 gctcgcttac cgttgtgtca tcccgaaacc gatccgcagg ggtttaaagc attacgccag
     3721 gcctatgaag aggcgctgcg actggcggta aatcctgtcg aggaagcaga tgatgaagaa
     3781 aaagatgcca ccgctgaaca tgaaatacta cgtgcattca ggacattact ggattcagaa
     3841 agtgatcgtt ttcagccctc cgcctggcag aaatttattc agcaattaaa tacctggaac
     3901 atggaggatg tcgatcaatt acgctggccg ctgtgtgcaa tcgctataga agcgcgatat
     3961 ctgtcattaa attgtgcttc tttgttggca gagcgtttga actggcattc gtttaatgac
     4021 agcgaaggaa tggatgagga agaaagggag gcttttcttg aggccattca ggctggtgat
     4081 tgtttcgatt tccttagcct tctggaatat cccgtcgcgt tacagaacca gactgttgaa
     4141 tattacttcg cgctggaacg ttgctgccgt taccatcctg actatgtcac tgcgtttttg
     4201 gcgatggaag gaccgtggtt cattcctgat gatgcaaagt tacatcgcaa actgttgcgc
     4261 tggtacagct cggtgcaaac aggtatggtg gaactcattc ctgtagccaa acagtggcaa
     4321 gcggaagaac cagaaagcga agatgcccgg tattaccagt gtgcacaacg tttgtactgc
     4381 ggcgaggggg aaagcctgct tgctgatctc ggcgcgtact gggaaagtta cccatctaca
     4441 caagctgata atttgttgtt gcagtggagc aagcgtcatt gcccggatta tttcgcgtta
     4501 ttagtgatgg ttatcgaagc gcggagcatg gtagatgcgc aaggtcaacc gctgaaatat
     4561 gttcctggtg agagcgcccg gacgcggctg ttatgggcgg aaattttaca tagcggaaaa
     4621 ttatcgccgt taggtcaatc gtttattgag tcgttattct tcaaacgcaa agcatgggcg
     4681 tggtggaaat cgagagtcgg tagcgagaca gaagaagatt caccgttact ggatttgtat
     4741 cgggtagcgg aacaggtagt acttgaagcg tttccgaaac aagagatgct ggcccgtctt
     4801 aatacaaggc tggaaggcgg agatgctcat ccattagagg ccattatcac ccggatgctg
     4861 ttgacgaaag taaaactcga gccggaggat gaagatgtcg atgagccaac acctgaaaac
     4921 catgaagaaa aaaatgatga gggtgaaaaa ccacagagca ttaccagcat tatcaaaatc
     4981 agtttaacgg tgctggttat aggttatgct ctcggcaaaa tcgcgatgtt gtttagttga
     5041 cgatgtgttt tttgcgccga tgccggatgc agcatttacg ccgcatccgg cagccgtgat
     5101 tatgaggtct caatggctaa ataaacgctc gcccgccgcc tgacctcatc gacgcgggcc
     5161 atatcgtttg actgcagcgc cgcatcaaaa tcggtgatca cccggccaat ctcttctcgc
     5221 tcgtcaccca gcgcccgcgc ccacaactct tccagtttgg ctttaaaggt gcgattgatg
     5281 agcatatcgc gcggataaat tttcaatgcg gataaccgcg tccgactctc ttcaatctgc
     5341 tgcgcgctca atgttaccgg gctgtggtta atcactctgg acttaacgct gccgtcttcc
     5401 agaagcacgt caacttcaag caacccgttg atatcataac taaagcgaat atcaatcgac
     5461 tgataagccc cggttttctt caacggcaca tcgaaggatt ccaccagaat gttgttttta
     5521 actttgtggt tttctccctg atagacgtta accgtaattg aatcctgttc cgggtgcatg
     5581 gttgaataag tttctacacg cgacacgggc acagtggtgt ttcgttcaat aatcggcgag
     5641 aaaatgccgg aaacgccctg gcggttaacc tcaacgccca acgagtaagg gcaaatatca
     5701 gtgaggatca cttcttcaat atcttcactg cgtaagcggc aggcggcctg gattgctgcg
     5761 cccagcgcga caatggtgct cggatcgtaa ctttgatacg gtaatttgcc aaacagacgc
     5821 acggcgattc gctgcaccag cggcatttgt gacgcgccgc caaccagcac cagactgtcg
     5881 atttgactcg gcttcagacg cgcatcgcgc aacgcctgtt caatcggcac tcgcaagcga
     5941 ttgagcagcg gcaaccacaa atcttccagt tcgttctcgt aaaattcgca ttcccgcgtt
     6001 tcatcctggt actaccagcg aatgtgcatt ggcgattgat tgctacattt tgccgcttcc
     6061 acacaggcgt acaaggctgc cagttcactc tcgttaagcg tggtccttgt gacatccgcg
     6121 cgctttaaaa cgtcatcgac caacatatgg gtaaaatctt cgccaccgag gaagttgtcg
     6181 ccagcggagg cgtgaacttc aatcaccggc gtggcgtact caagcaccgt aacgtcaaac
     6241 gtgccgccac cgagatcaaa caccagcgaa cgggtatttt gttgggtatg caggccatac
     6301 gccatcgcag ctgctgtggg ttcattaatt aagcgtaccg catttaaccc ggctaagtcc
     6361 gctgctaaac gggtatgctt gcgttgttca tcgctgaaat aagccagaac ggagatcacc
     6421 acatctttaa tcggacgttg cagaaattct tcggcatctt cttttaatga gcgtaatacc
     6481 aaagaggaca gttctggcgc gttaaatgtg tcgctgccta accgccagtt ggtattactg
     6541 cccattgcac gtttaaataa cgctgccgtt ttatccggat gcgaagtacg ccgtgataca
     6601 gccggttttc cgactaaaat atgattattt tcatccatgc taattatgga tggtgttaaa
     6661 tattcaccga acttatttgg aattaattgc gcggcaccgt ctttccagac ggcaattaaa
     6721 ctattggtag tgccgagatc gatacctatg gcgagttctg cattatccat tgcatttata
     6781 tcttgaaaga aaaagagata aatagattaa gaccacccag gtaacgcgtg gtgatcttaa
     6841 tcaatgacga gtgttaagcg taaaatttca gacgatcagc cagtaaatca acaaagccct
     6901 gacgatcaac atcgacaatt acagtggcat tcggtttatt gccagtcaga taataataat
     6961 caacaaccgt cataccctgg gtatatttcc cctgtgtttc cacgccaacc cagcgctcaa
     7021 cagtggtaaa caactcgggt ttcaacagcc aggcgatggt gcatgggtca tgcagcggtg
     7081 cgccgacaaa gccccatttt tcgtctttat gatattcgag gaagaaatcc agcagttcgg
     7141 caacaatggt tgaaacaggg ttgccaatcg cgcggaaacg ctcggtgtct tcaacgtgga
     7201 tttgcgcttt atgagtaaca tccagaccgg ccatcaccac cgggatccct gactggaaga
     7261 caatttctgc cgcttccggg tcaacgtaaa tattaaattc agccgcaggc gtccagttac
     7321 caagccccat tgcgccgccc ataatcacga tacgggcaat tttgctatgc agttccggat
     7381 ggctattgag cagcaaggca acgttagttt gcggtccggt agaaacaatc gtgacaggtt
     7441 ccgcactttc gcgcagcgtt ttcgccatca gctccaccgc cgtacagttt tgcggtgcga
     7501 acgtcggttc cggtaatgcc gggccgtcga gaccgctttc gccgtgcaca ttgtccgcga
     7561 taatcaactc acgcattaac ggttttaccg cgccgcctgc caccggaata tcggtgcgat
     7621 taagcaaggt cagcatacgc agaacattgc gtaaggtttt ttctggtgtc tggtttccgg
     7681 cggaagacgt aattgctttg acatcaagct ctggtgaggc gagtgcgaga actattgcga
     7741 tagcgtcatc atgacctggg tcgcaatcta acagaattgg cagtgccatt gttgctcctt
     7801 gttgtgtgct tctttgcgac aagggtaacg ccaggatgta acagatacga ggggcgaaac
     7861 gataaagcgt gagatggcgc gcaattgggt atgtgcgcca gagtgattaa tgcaggattt
     7921 tcgcgaggaa gtcttttgcg cggtccgatt tcggatcatc gaagaaagcg tctttcggcg
     7981 agtcttcgac aattttaccc tcgtccataa agatcacccg attcgccact ttgcgggcaa
     8041 agcccatttc gtgggtcacc accatcatgg tcattccttc gttcgccagt tccaccatca
     8101 cgtccagcac ttcgttgatc atctccggat ccagcgccga tgtcggttcg tcgaacagca
     8161 tcgcaatagg agccatacac aatgcgcgag cgattgccac gcgctgctgc tgaccgccgg
     8221 aaagctgcgc cggaaattta ttggcatgtg cggaaagtcc cacgcgctcc agcaatttca
     8281 atgctttttc acgagcgggc gctttatcgc gtttaagcac tttcacctgc gccagggtca
     8341 ggttttcgat aatcgacaga tgagggaaca gctcgaaatg ctggaatacc atcccgacgc
     8401 gggagcgcag ctttgccaga tcggttttct tgtcgttaac cacgatacca tcgacggtga
     8461 tttcaccttg ctgcactggt tcgaggccgt tgacggtttt aatcagcgtt gatttgccgg
     8521 aaccagacgg gccgcaaacc accaccactt cgcctttttt cacttcggtt gagcagtcgg
     8581 tcagcacctg aaagtgacca taccattttg aaacattttt cagggtaatc attatgctgt
     8641 ccttcttttc aagtagctga ccaacaacga cgcgctaagg ctaataacga aataaacaaa
     8701 tccggcaaac aggatcatct cgacctgcgt accatcacgc tcaccaatgg ttgaggcggt
     8761 acggaagaaa tcggccagag ataacacata caccagcgag gtatcctgga acagtacgat
     8821 gccctgagtg agcagcagcg gcaccatcgc gcggaaagcc tgcggcagaa taatcagttt
     8881 catcgactgc cagtgagtca tccccaacgc cagcgcggcg ctcgactgac cacgggaaat
     8941 actttgaata ccggcacgga taatctctga atagtaggcc gcttcaaaca tcgaaaacgc
     9001 caccatcgcc gaaattaaac gaatatcatt ttttggcgat aatcccagca cattttgcag
     9061 aaatcccggc acaatcaagt aaaaccacag caaaaccatg actaaaggaa tcgagcggaa
     9121 tacgttaacg taggctttgg caaaccacga aacaggcgcg aagctggaca agcgcatcac
     9181 cgccagcatg gtaccccaca aaataccaat cacgactgcc gtgacggtga ttttcagggt
     9241 gatcaccagc ccgtcgagca gatatggcag ggaaggaaca atggaactcc agtcaaactc
     9301 gtacattatt tgccccccat gttgccgggc aggcgaactt tacgttcaac cagcgtcatc
     9361 accagcatga taaaagcgtt aatcaacaca tacgcaagcg tgatggcggt aaacgactcc
     9421 caggcgtgag ccgagtagtc gagcaattta cccgcctgcg ccgccatatc caccagaccg
     9481 atggtcgagg cgatggcgga gtttttcacc aggttcatca tctcggaagt catcggcggg
     9541 acgataacgc gataagcatt aggcagcagt acgtatcgat aagtctgcgg tagcgtcagg
     9601 cccatcgcca gcgcggcatt cttctgccca cgcggtagcg actgaatagc ggcgcgtacc
     9661 tgttcgcaaa cacgggcggc ggtaaacagc cccaggcaga gcatggatga aaggaaaaac
     9721 tgaatattgg gatccagctc ggccttaaac cacatgccga ttttctccgg cagcagctcc
     9781 gggatcacca gataccaggt aaagaattgc acaatcaacg gcacgttgcg gaacagttcg
     9841 acatacaacg tacccagacc ggagagaaaa cggtttggaa cggtacgtaa aatgccgaaa
     9901 aatgaaccga cgaggaaagc gataatccag gcgcagatcg acaaagcgat cgtcacctga
     9961 aaaccactcc agacccaacc aagataggtg gtattgccga acggggcttg ttgtaaaaaa
    10021 ataccccagt tccagtctat agacataaat ctactccaga aaaaagaggg tagcagcgtt
    10081 aactgctacc ctcgaagatt gttacccagc gtattgcggt tttcaggccc gatggggaac
    10141 gaccatcagg cgtatagtct gtccgtgcta cgtaacaatc gagagggctg gaatttccgc
    10201 ccctggttct tgtaattagt tcagtgcctt gtcattcggt tctttgaaca gtgctttcat
    10261 ttcgtctgac agttcgaaat tcatgttcag gtttttcggc ggaattggat ttttgaacca
    10321 tttatcaaac catttttccg cttcaccgga ggtctgcacc tgagcgatag tgtcatccat
    10381 cagctttttg aactgcggat cgtctttacg caacatgcaa ccgtaggctt cctgagactg
    10441 cggcttgccg acgatatccc agttgtctgg tttcttcgct ttcgcacgtt caccggccag
    10501 cagagcgtca tccatcataa aggcaacggc acgaccgctt tccagggtgc ggaaagagtc
    10561 accgtgatct ttggcgctga tgatgcgcat attcattttt tgctcttcat tcagtttgtt
    10621 gagcaaaact tcagaggtag tgccggaagt gacgactacg gctttgcctt tcaggtcggc
    10681 aaaatctttg atatcgccgc cctttttggt caacaggcgc gtaccgacca cgaaaatagt
    10741 gtcagagaaa gccgcctgtt tttggcgttc gacgttgttg gtggtagaac cacattcaaa
    10801 atcgaaagtg ccgttttgca gcagtggaat acgattttgt gaggtaatcg gaatcagttt
    10861 tacctgcaag tccggtttgt tgagtttctt tttcactgct tcaacaatgg cgttggagta
    10921 atcctgcgag taacccacca ctttttgctg attgtcgtaa taagagaaag gtactgaaga
    10981 ttcacggtga ccgacgacaa tcacaccgtt tttggcgatt ttgtccagcg tactgcctgc
    11041 tgccggggcg gcgtcatctg cctgtgccag tcctgcggaa agcgccaggg cgaggattgc
    11101 tgtggcaggt ttacgtaatt gcatatccaa cacctttatc ttctgcgtta agaacgcatg
    11161 gatacccgtt gtgagtgttt gtgttgttat cgtctgcaac tttattgtgc agtgttgtgc
    11221 ctgttaggta acatttagtt tggctaaatg taaagatatt gatgttttat tgtttgtttt
    11281 tgcgaggtgc accgcaccat tctgaagcaa aattcttaaa atgcactctt ttggtgctac
    11341 cggtggattg ctgtggtgca accgggttgt actgatgctg gttcaagctt tgccttgtat
    11401 aacaaagcaa tagatgtgcc aaagttggat aggagaatat tgttatccgg ataatgcact
    11461 gatgccgcat ccggtgagcg tgccgaaata tgggatgtat tccggcacga taagaaggga
    11521 ttatttacgt cgctgacgca gactcatcaa cacagcagca aaaccaaaca gtgccgtcag
    11581 cacccacagc ggccagttgc cggtacgtgc gtatggtgtg agtccggtgg tcggcgtcac
    11641 gttagtggtt aacacctcgc gggcgaactg cgggatcatc gcctgaatct caccctgcgg
    11701 gccaatcacc gccgtaatgc cgttgttggt gctgcgcaac agtgggcgcg ccagctccag
    11761 cgcacgcatt cgcgccatct ggaagtgttg ccatggacca atagatttac caaaccacgc
    11821 atcgttggag atagtcagca ggtagtcggt atccgggcgg aagttatcgc gcacttgctc
    11881 ggcgagaata atctcgtagc aaatagccgc agtaagctca ataccatttg ccgacagcgg
    11941 cggctggata tatggcccac ggctgaacga cgacatcggc agatcaaaga acggtgctaa
    12001 cggacgcaga atcgactcca gcgggacaaa ctcgccaaac ggcaccagat ggtttttgtt
    12061 atagcgatcg gctgattcgt agctgtacgg tgcaccttta cccagcgtga tgatggtgtt
    12121 gtaggtatcg tagcggttct gcttattgag acgcgcgtcg acaatcccgg ttaccagcga
    12181 gctaccttta tcacgcaact caccgtccag tgctttgagg aacggttgct gattaatttc
    12241 cagatcggtt atcgccgact ccggccagat aatcaacgat gatttgccca tcagcggtgc
    12301 cgttgcgttg tagtaaatct ttagcgtatt aagaagctgg ccttcgtccc atttcagcga
    12361 ttgcggaata tcgccctgaa ccatcgaaac ctgaatggtt ttctccggtt gcggggtaaa
    12421 ccattggatg taacgcagcg ggaagggtag ggcaaacagc acgacggcca ccaccagcgg
    12481 acgccagttg cgtttgacca acgccagtgc cagcaggcca ctaaccatca tcagcaggaa
    12541 gttaatggct tccacgccca ttagcggtgc cagccctttt aacgggccat caatctggct
    12601 atagccgaac tgtaaccacg ggaagccagt cagcacccaa ccgcgcagaa actcggtcac
    12661 ttgccagaga gcaggggtgg caatcgccac gcgcagccag gtggttttcg gccacagacg
    12721 cgacagcacg ccagcaaaca gtccggtata cagcgacaaa tacgccgcca gcagcaccac
    12781 caggaagatg ttaaccgggc caggcatccc gccaaaggtc gcgatgctga catagaccca
    12841 gttaataccg ctgccaaaga ggccaaatcc ccagcaaaag ccaatagcgg cagactggag
    12901 tgggcgacgg ttaaaggtca acgcctgaag ccccatcagc gaaataatcg ccgcaggcca
    12961 gacgtcgtaa ggagagaagg ccagcgttcc gcaggcaccg aataataacg ccagcagcag
    13021 gcgaatgcgc tggcgttcaa ttaatgaggc aaaatccatg tagttatcta tccagtttcg
    13081 gttttattca tccagctttg gctggggtga gtcatccggg attttgacat gaacctgaat
    13141 aatacgccga ctatcggcca tcgccacttt gaactggtaa ccgtcgatgt cgatagtttc
    13201 gccacgcgcc ggaagatgcc caaacgcctg catcaccaga ccaccgatag tgtcgacttc
    13261 ttcatcgcta aagtgggtgc cgaacgcttc gttgaagtct tcaatggaag ccagtgcgcg
    13321 cacggtccag gtatgacgac tcagctgacg gaagtcgata tcatcttctt cgtcatactc
    13381 gtcttcaatc tcaccaacaa tcagttccag gatgtcttca atggtcacca gaccggaaac
    13441 cccaccgaat tcgtcaataa cgatcgccat gtggtaacgc tgagagcgaa actctttcag
    13501 catccggtct acgcgcttac tttcaggaac gacaaccgcc tgacgtaaca ctttgtccat
    13561 gctgaaggct tcagcatcgc tgcgcataaa cggcagcaag tctttcgcca tcagaatccc
    13621 ttcaatgtga tctttgtctt cgctaatcac cgggaaacgt gagtgggcgg actcgatgat
    13681 gacatcaagg cattcgtcca gcgtctggtt gcgtttcagg gtaatcatct gggagcgggg
    13741 gatcatgatg tcgcgaacgc gttggtctgc gatgtccatc accccttcga gcatatcgcg
    13801 cgtatcttcg tcgataaggt cgttctgccc ggaatcacgg atcagcgcca gcagttcgtc
    13861 acggtttttc ggttcaccgt ggaaaagttg gctgagtaac agggagaaaa atcccttctt
    13921 gttgcttatc gtgtcactac tgtgtgaatt gtcgtcgctc atggcgtcgt atgggttctc
    13981 atgttagtta atcaaaacgc cgtcgttaat caccaacggc ggggacgtct gccagtcaaa
    14041 tgcctggcaa attattcttt ctcggcaatg tacggatcct catagcccag agcaagcata
    14101 atctctgttt cgagggcttc catttcttct gcttcgtcat cttcaatgtg atcgtaacct
    14161 aacaaatgca gactgccgtg caccaccata tgcgcccagt gcgcctccag tggtttgcct
    14221 tgctcctgag cttccttctc aaccacctga cggcagataa ccagatcgcc cagtagcgac
    14281 atctccatgc caggcggcac ttcaaacggg aaggagagca cgttggtcgg cttatcctta
    14341 ccgcgatagg tcagattcag actgtggctt tcggcggtat cgaccacgcg aatagtcact
    14401 tccgactctt cctgaaactg cgggatcacc gcattcagcc atgtttgaaa ctggctctct
    14461 tccggtaacc cggaattatc ttcacatgcc agttgtaaat cgaggatcac ctgactcatt
    14521 tttgttcctg ttcttcgcgc ttgcgttctg ctgccagcgc cgctttacgt ttttgttctg
    14581 cttcttccca ggcttcataa gcgttaacga tacgcgccac caccgggtga cgaaccacgt
    14641 cttcgctgtg gaagaagtta aagctgatct cttcgacatc ggccagcact tcaatggcat
    14701 gacgtaagcc tgatttagta ttacgcggca ggtcgatctg tgtgacgtcg ccggtgataa
    14761 ccgcttttga gttaaaaccg atacgggtca ggaacatctt catctgttcg atggtagtgt
    14821 tctggctctc atcgagaatg ataaacgcgt cgttcagcgt acgaccacgc atataggcca
    14881 gcggcgcgac ttcaataacg ttgcgctcaa tcagtttctc gactttctca aagcccagca
    14941 tttcaaacag cgcgtcgtac agtgggcgca gatacgggtc tactttctgg cttaaatcgc
    15001 caggcaggaa gcccagtttc tcaccggctt cgaccgccgg acgagtcagc agaatacggc
    15061 gaatttcctg acgctccagg gcatcaactg ccgcagccac tgccaggtag gttttacccg
    15121 tacccgccgg gccaacgccg aaggtaatgt catggtcgag aatattggcg atgtactgcg
    15181 cctggtttgg cgtgcgcggc ttaattacgc cgcgtttagt tttgatattg accgctttgc
    15241 cgtactccgg cacgctctcc gcgctttgct ccagtacccg cgcttcttta atcgcaaggt
    15301 ggatctgttc cggttcgata tcctgaatct gaccgcgcat cggggcagta tcgacataca
    15361 ggctacgcag aatgtctgcc gcagcggtga cgcaaatcgg acggccggtc agtttaaagt
    15421 ggttatcgcg gcgattgatc tcgatgccga gacggcgttc gagctgcttg atgttgtcat
    15481 caaacgggcc gcacaggctc aacagacgcg cattgtctgc tggctccagg gtgatttcgc
    15541 gagtgtctat gttcaaaccg ttcctcttat ctgtatgccg ccggaagctg aacattcacc
    15601 ggcctataag gaaattattc acgccacagg aaaaaggcgc aagcattgcg ataaagatgg
    15661 ggataaagag agaaaaaaca aggcccaccg gaacggcagg cctgagaatt acggctgata
    15721 ataacccacg ccaaggtcgt tttctttgcg ggtgcgggca atcaccgact ccggtgtttc
    15781 tgccatgcgc agacccattt catcttcagt tcgcaccact ttaccgcgca gagagttcgg
    15841 gtagacgtcg gtaatttcta catcgacgaa tttaccgatc atatccggtg tgccctcgaa
    15901 gttgaccacg cggttatttt cggtacgccc ggaaagctcc atgatgctct tacgcgaagt
    15961 cccttctacc agaatacgct gggtggtgcc gagcatccga cggctccatg ccatcgcttg
    16021 ctgattgata cgttcttgca gaatatacag acgctgcttc ttctcttctt ccggaacatc
    16081 atcaaccata tcggcggcag gcgttcctgg acgtgcagag aagataaagc tgtagctcat
    16141 gtcgaaattg acgtcggcaa tcagcttcat cgttttctcg aagtcttcgg tggtttcgcc
    16201 agggaagcca acaatgaagt cagaactgat ctgaatatcc ggacgcgccg cacgcagttt
    16261 acggatgatc gctttgtact ccagcgccgt atgggtacgt cccatcaggt tcagaatgcg
    16321 atcggaaccg ctctgtaccg gcagatgcag gaagctcacc agctcaggcg tgtcgcggta
    16381 cacttcgatg atatcgtcgg tgaattcgat cggatggctg gtggtaaagc gaatacgatc
    16441 gatcccgtcg atcgcagcaa ccagacgcaa cagatcggca aacgatccgg tggtgccgtc
    16501 gtagttctca ccacgccagg cattcacatt ctgaccgagc aggttgactt cacgcacgcc
    16561 ctgagccgca agctgggcaa tctcaaacag aatatcgtca gacggacggc ttacctcttc
    16621 accacgggtg taaggcacca cgcagtaggt gcaatattta ttgcagcctt ccatgatgga
    16681 gacaaacgcg gtcggccctt cggcgcgcgg ttccggcaga cggtcaaact tctcgatttc
    16741 cgggaagctg atatctacaa ccgggctgcg gtcgccgcgc acggagttga tcatctccgg
    16801 cagacggtgc agcgtttgcg gcccaaaaat aatatcgaca tagtgggcgc gctggcgaat
    16861 gtgctcgcct tcttgcgatg ccacgcagcc accgacgccg ataatcaggt ctggattctt
    16921 ctcttttaac agtttccagc gacccaactg atggaagact ttttcctgag ccttctcgcg
    16981 gattgagcag gtgttcagca gtagcacatc cgcttcttcc gccacgtcgg tcagttgata
    17041 gccgtgggtg gcatccagca gatcggccat cttcgatgaa tcgtactcgt tcatctgaca
    17101 gccccaggtt ttaatatgga gttttttggt catcgacttg ctcttgcgaa atagtagcca
    17161 ggaatgcagg gcgcatagtg taatgctttg ctgccgttgt gaccagtatg agcgttatca
    17221 gcccttgggg gtaaaaatcc tgtaaactta aagcatattg ctaacaggat gattgaccat
    17281 gacaaatcaa ccaacggaaa ttgccattgt cggcggagga atggtcggcg gcgcactggc
    17341 gctggggctg gcacagcacg gattttcggt aacggtgatc gagcacgcag aacctgcacc
    17401 gtttgtcgct gacagccaac cggacgtgcg gatctcggcg atcagcgcgg cttcggtatc
    17461 attgcttaaa gggttagggg tctgggatgc agtacaggct atgcgttgcc atccttaccg
    17521 cagactggaa acctgggagt gggaaacggc acatgtggta tttgacgcag ccgaattaaa
    17581 gttgccgttg cttggctaca tggtggaaaa cacagtgctg caacaggcgt tgtggcaggc
    17641 gctggaagcg catccgaaag taacgttacg tgtgccaacc tcgctgattg cattacatcg
    17701 agataatgat cttcaggagc tggaactgaa gggcggtgaa acgatccgcg cgaaactggt
    17761 gattggtgcc gacggcgcaa attctcaggt gcggcagatg gcgggaattg gcgttcatgc
    17821 ctggcagtat gcgcagtcgt gcatgttgat tagcgtacag tgtgagaacg atcccggcga
    17881 cagcacctgg cagcaattta ccccggacgg accgcgtgcg tttctgccgt tgtttgataa
    17941 ctgggcatcg ctggtgtggt atgactctcc gacgcgtatt cgccagttgc agaatatgaa
    18001 tatggcgcaa ctccaggcgg aaatcgcgaa gcatttcccg tcgcgtctgg gttacgttac
    18061 accgcttgcc gctggtgcgt ttccgctgac gcgccgacat gcgttgcagt atgtgcagcc
    18121 ggggcttgcg ctggtgggcg atgccgcgca taccatccat ccgctggcgg ggcagggggt
    18181 gaatcttggt tatcgtgatg tcgatgccct gattgatgtt ctggtgaatg cccgcagcta
    18241 cggtgaagcg tgggccagtt atcctgtcct caagcgttac cagatgcggc gcatggcgga
    18301 taacttcatt atgcaaagtg gtatggatct gttttatgcc ggattcagta ataatctgcc
    18361 gccgctgcgt tttgtgcgta atctcgggtt aatggcggca gagcgtgctg gcgtgttgaa
    18421 acgtcaggcg ctgaaatatg cgttagggtt gtagccttac aacattgccg gatgcgtgcc
    18481 agccgtaggt cggataagac gcggcagcgt cgcatccgac attgaaggat aagacgtgtc
    18541 aacatcgcat tcgacattga atgaacgcag aaaagcaaaa agctcgccga agcgagcttt
    18601 tttaat
//
LOCUS       DAJTUP010000080        18441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTUP010000080 DAJTUP010000000
VERSION     DAJTUP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.080"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(62..979)
                     /gene="lpxC"
                     /locus_tag="OO468_002823"
     CDS             complement(62..979)
                     /gene="lpxC"
                     /locus_tag="OO468_002823"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR6259873.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(1080..2231)
                     /gene="ftsZ"
                     /locus_tag="OO468_002824"
     CDS             complement(1080..2231)
                     /gene="ftsZ"
                     /locus_tag="OO468_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR6259874.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(2292..3554)
                     /gene="ftsA"
                     /locus_tag="OO468_002825"
     CDS             complement(2292..3554)
                     /gene="ftsA"
                     /locus_tag="OO468_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR6259875.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(3551..4381)
                     /gene="ftsQ"
                     /locus_tag="OO468_002826"
     CDS             complement(3551..4381)
                     /gene="ftsQ"
                     /locus_tag="OO468_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR6259876.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(4383..5303)
                     /gene="ddlB"
                     /locus_tag="OO468_002827"
     CDS             complement(4383..5303)
                     /gene="ddlB"
                     /locus_tag="OO468_002827"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6259877.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(5296..6771)
                     /gene="murC"
                     /locus_tag="OO468_002828"
     CDS             complement(5296..6771)
                     /gene="murC"
                     /locus_tag="OO468_002828"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR6259878.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(6825..7892)
                     /gene="murG"
                     /locus_tag="OO468_002829"
     CDS             complement(6825..7892)
                     /gene="murG"
                     /locus_tag="OO468_002829"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR6259879.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(7889..9133)
                     /gene="ftsW"
                     /locus_tag="OO468_002830"
     CDS             complement(7889..9133)
                     /gene="ftsW"
                     /locus_tag="OO468_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR6259880.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(9133..10449)
                     /gene="murD"
                     /locus_tag="OO468_002831"
     CDS             complement(9133..10449)
                     /gene="murD"
                     /locus_tag="OO468_002831"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR6259881.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(10452..11534)
                     /gene="mraY"
                     /locus_tag="OO468_002832"
     CDS             complement(10452..11534)
                     /gene="mraY"
                     /locus_tag="OO468_002832"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR6259882.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(11528..12886)
                     /gene="murF"
                     /locus_tag="OO468_002833"
     CDS             complement(11528..12886)
                     /gene="murF"
                     /locus_tag="OO468_002833"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR6259883.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(12883..14370)
                     /gene="murE"
                     /locus_tag="OO468_002834"
     CDS             complement(12883..14370)
                     /gene="murE"
                     /locus_tag="OO468_002834"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR6259884.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(14357..16123)
                     /gene="ftsI"
                     /locus_tag="OO468_002835"
     CDS             complement(14357..16123)
                     /gene="ftsI"
                     /locus_tag="OO468_002835"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR6259885.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(16139..16504)
                     /gene="ftsL"
                     /locus_tag="OO468_002836"
     CDS             complement(16139..16504)
                     /gene="ftsL"
                     /locus_tag="OO468_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR6259886.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(16501..17442)
                     /gene="rsmH"
                     /locus_tag="OO468_002837"
     CDS             complement(16501..17442)
                     /gene="rsmH"
                     /locus_tag="OO468_002837"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR6259887.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(17444..17902)
                     /gene="mraZ"
                     /locus_tag="OO468_002838"
     CDS             complement(17444..17902)
                     /gene="mraZ"
                     /locus_tag="OO468_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR6259888.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         4375 a         5398 c         4443 g         4225 t
ORIGIN      
        1 cacctggccg gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc
       61 gttatgccag tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga
      121 aggtcacata ttcccaggct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca
      181 gtgcatgacc ggatttataa gcggtaaatg caccaataat attgtgacca cacatgaaca
      241 agtcaccgat cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt
      301 cttcgttcag tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc
      361 acaaaccacg ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc
      421 tgatctggcg cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat
      481 caatagccgg atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa
      541 attcagccca cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt
      601 tggcgcagtt caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc
      661 tgccgtccat gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca
      721 ggcccgcgag agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga
      781 ccagacacgt acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg
      841 gtggattcaa gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg
      901 tcagggtgac tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa
      961 gtgtcctttg tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact
     1021 atacattcgg cgggccagtt tagcacaaag agcctcgaaa cccaaattcc agtcaattct
     1081 taatcagctt gcttacgtag gaatgctggg atatccagat aatccggctc tttcgcagtt
     1141 tgcggcgcat tgtcattcac tactttagca accggcttct gctcctgggt cagcggagcc
     1201 atcccatgct gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga
     1261 gtgatttcag gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg
     1321 tcattcatat ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat
     1381 gcacggatgg tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg
     1441 atgttaacca gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag
     1501 atagccattt cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc
     1561 attgcgtagc ccatttcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa
     1621 cccggacgag taatcagttc ggcgatacct tgcacagcgc ctttcagtac atcgttcgct
     1681 gcgccaaacg catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc
     1741 gggatagtga tcagagagtc cacatgcttg gacagttcag tgatcccctg ctcggcgaat
     1801 gccatacgct tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata
     1861 cccaaatctt ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc
     1921 atacccgcag caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga
     1981 tcctcatcag ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg
     2041 ataccgctac cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg
     2101 gtatttaccg cgaagaattc aacaccttca atacgctcgc gcaccatgtg ttcaacagca
     2161 ttaccgccgc cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt
     2221 ggttcaaaca tagtttctct ccgatttgtg cctgtcgcct gagtccgtaa tcatcgtcgg
     2281 cctcataaaa attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca
     2341 actgatgctg taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca
     2401 tagtgaagca atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg
     2461 gtaatgttca gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct
     2521 gcaagacctt cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt
     2581 ttaacccctt gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg
     2641 agcaactcgg tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgc
     2701 ggcggacgac cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc
     2761 agcgcacaac cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg
     2821 taagcgatat cactggtcac gacattgcca gcataaggaa ttacctttgt gtggcgcaat
     2881 gccccaccgg tataaacggc gatatccatt gtaccaccac cgatatcgac gacgcagaca
     2941 cccagttcac gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc
     3001 agttggtcaa ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg
     3061 ttgtgacatg tgatcaggtg cacttttgcc tgcatccgca cacccgaaag tcctaccgga
     3121 ttcttgatcc cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga
     3181 tgctcatcgc gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc
     3241 gtcacttctt cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta
     3301 ccagaaagcg ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca
     3361 atggcgcgtt gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata
     3421 ccacgtgacg ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc
     3481 cctactaaag cggcaacctt cgcggtacca atttccagtc ctactaccag ttttctgtcc
     3541 gtcgccttga tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg
     3601 gcggcaaggg cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa
     3661 tccgtttgcc atcggtttgc gcctgctgct gtaaaaccgg ataaagttct acaaagcgaa
     3721 ccaaacgttt catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca
     3781 actgccagga acgccgcgcg gtcatcgccg cttccttcag agtaaatctg tcctttgcca
     3841 gcatctgccc catttcgcga tagccctgca acacttcatt agcgctgcct tccgggccat
     3901 acagcattgg aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt
     3961 ccgcgtctac catgtgttga tcattccacc gcgcaatcgg cacatattca accagatgaa
     4021 tcttcaattc atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt
     4081 gttctatttg cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca
     4141 atgccaggat cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca
     4201 ccagctttga gagcggcagg cgttgcgcat cttccatcca gctcaacacg acccagccgc
     4261 tcaccaacac tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat
     4321 tattgcggcg agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca
     4381 tattagtccg ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga
     4441 cgtgccgcca tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc
     4501 agataaaact gtccatcact gtccagcata acgtcaatac gtccccatcc tttgcaacct
     4561 aacgtcgtcc atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcttccaga
     4621 cctgcggggc agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag
     4681 gttccggacg gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg
     4741 aactccggcc cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat
     4801 cttaatgcat cttgtagagc attttctgct actacttttg acattcccac actggaacct
     4861 tcgcggctcg gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta
     4921 tcgctcaggc ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa
     4981 ccggcacctt gccacagaag tttgctgcgt agtttatcta ttgaaagcgc agatgccatc
     5041 actccgctgc cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct
     5101 tcaccgccgc gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc
     5161 gtcacgtcga cttctttcgg gtcgacagga tacgcatcaa taccgccttc acgcagcccg
     5221 gctaacaccg ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac
     5281 aggaccgcga ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat
     5341 ttcagctaaa gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc
     5401 gttaccggtt aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat
     5461 gggatcaatt ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg
     5521 aattggcgct tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag
     5581 cacattggcg aaatcatcat acaggtcgcg cgtacgggta aaacggtgcg gctgaaacag
     5641 cattaccagg tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc
     5701 cgtcgggtgg tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg
     5761 ctccagcggg aattcaccga ggaaatcaaa acggcgacca gtcccctgga agctctccag
     5821 cgcacgcaaa atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc
     5881 ggcgttcagc gcgttatgac ggcctggcgc attcagggtg acgcacatcg gctctttgtc
     5941 ctgacgcagc agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac
     6001 gtcggcatct tcgctgaagc cgtaagttgt ggtctgacgt cccacacgcg gtaacaattc
     6061 gcggatcacc ggatcatcaa cacacatcac cgcacgaccg taaaaaggca ggttgtgcag
     6121 aaaattaata aaggtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc
     6181 ggcttcgata ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact
     6241 ctcatctgct tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc
     6301 ttttaccagc ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga
     6361 aaccatcgcg gtagttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa
     6421 acgcattaac tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc
     6481 tgcgacaatt tccgggttgt cggcagaaat ggcgctggaa acaacgacca cgctggcatc
     6541 acgtacgttt tccgggcgat ggttgaaata aatcgtcgca cccagattca gtaactgctg
     6601 cgtgaccgga tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac
     6661 ttcggcaata ccgcccatac cggcaccacc aatgccgaca aaatgtatgt gccgaacgcg
     6721 acgcatttcg ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc
     6781 cattaacttc ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc
     6841 cggctcactt catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt
     6901 tctgccatgg ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc
     6961 acgctaagct gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca
     7021 ttccagtatt gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc
     7081 gcggcgattt cactcacagt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca
     7141 tacgccgccg ccatatcatc aataaattca gtcactttat gctgcggttg ccccgcttcg
     7201 gcatacgcct gttcaacgga ttgttgcgaa cctttgccgc tctgatgcca gatagtgact
     7261 gaatcaccca gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg
     7321 gaaccaccca ctaccaacac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc
     7381 aacggcagcg ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa
     7441 gcgcctggaa acgcctgcat cactttggta gcaatcttcg ccagccattt attggttaag
     7501 cccgcaatac cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga
     7561 ccacctggac ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc
     7621 ataatcgccc gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct
     7681 tttatacctt ttccacgcaa accagagata cgaatgaaat caatttcgat gccatgtttt
     7741 ggcactaagt ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga
     7801 gccattagat agtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc
     7861 atcaccatta atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt
     7921 tctccagacg cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca
     7981 gtaagctcga accaccgtaa ctgattagcg gcaatgtcag acctttggtc ggtaacatcc
     8041 ccgccgccgc gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg
     8101 cgagaaaacc ggaaaaacgg tggtcaattt ctaatgcttt gcggccaatc gacatcgcac
     8161 gaaaagcgac gaagaatacc attaaaagtg ctagtaccac accgacataa cctaattctt
     8221 cgccgataat ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta
     8281 ccgaattacc taaaccttgc ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg
     8341 ttaactgata gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc
     8401 ggatacggta cggttcggcg aggatcagca acacaaccgc tgaaatgccc ataccgataa
     8461 tggcaatgaa ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa
     8521 acaacaccac caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa
     8581 tcacgcccat cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca
     8641 ccagatagtt ggcgatatag caaaacagcg acagttttgt cagctccgca ggctggatac
     8701 gcagcaaacc gagatcgatc caacgcgatg cccctttaac agcgctaccc actaccagca
     8761 cgatcatcag caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact
     8821 ccatcggcag acgcagcgta atgatcgcca gaataaacgc caaaatcaga tagacaccat
     8881 cacgcttcgc gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg
     8941 aggtcaccat gataaagcca atcgccgcga ggccgaaggt cagccacagt aaggtgcgat
     9001 cgtacatgat caggctgtcg gtatcttttt cccgcgagcc catcacccag ccctttagcg
     9061 ccgtggagat ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga
     9121 gagataaacg catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc
     9181 aaagttcttg aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg
     9241 ctgaacacgc ggagccagca agcgcatagc ctgttccata gtttcggttt gttctgccac
     9301 ttccgggcgt agcgccgcca gctgcgcgcc gtcacgaccg aaacaataca gacgtacgtt
     9361 atcgccattc aggtaacgca ccagtgggct aaagtccgcc gatttaccat cgccgcccag
     9421 caacaaatgc agcgtgccgt ctacgtgcag gccattcagc gccgcttccg tactgccgac
     9481 gttggtcgct tttgaatcgt taatccagcg cacaccgtta tgctccagca ccacttcaaa
     9541 gcgatgcggc agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggtaaccc
     9601 tgcggcatct gccagcgcca gcgccaccag cgcattggtg tagttatgct gcccggaaag
     9661 tttcatctct ttcacattca gcactttctc gcctttaacc cgcagccagg tttcgccctg
     9721 ctgatgattc aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatccgc
     9781 accgcgaatc ggcattgtta aggcatcatc agcattaacc acgcaaactt tcgcgttttc
     9841 gtaaatgcgc agttttgctg cacgatactg ttgtaaacca aacgggtagc gatccatatg
     9901 atcttcagtc acgttcagaa tggtcgctgc taccgcctgt aagctggagg tggtttccag
     9961 ctggaagctc gacagttcca gcacgtacag ttcacactca tcatccagta gcatcaacgc
    10021 aggcaggcca atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc
    10081 cactagcgtg gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg
    10141 tgcttcgcga caaaacagct cgatatcgcc aacgatttcg attccggcat cagcggcagc
    10201 gcttaaggat ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat
    10261 cagccattca tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag
    10321 gccaggcggt gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa
    10381 gtccacgcag gaaagcccgg tgaggcccag gccgataatg acaacatttt taccctggta
    10441 atcagccatg attaacgtac cttcagcgtt gccagaccaa tcaggaccag catcagcgaa
    10501 ataatccaga aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga
    10561 tgaatcggtg ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg
    10621 atgacagaaa gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga
    10681 cgtagcagta cggcgataat gcctaacgca ccacctaacg ccagcgaacc gacatcgccc
    10741 ataaagacct gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc
    10801 gcggtacaga caataaccag ttccccggcg tgtcgcagat acggtatgtg taaatagctg
    10861 gcaaagttca tgttgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg
    10921 gtcggcataa ttgccagacc gtcaagacca tcggtcaggt ttaccgcgtt gccagtaccc
    10981 acaatgacga agtaagccag cagaatgtag aacagcccca gttgcggcat cacatcttta
    11041 aagaatggga ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta caaagcgaaa
    11101 gcaacaccca gcgcaatgac cgacatccag aaatatttcc aacgagcgat caaccctttg
    11161 gtgtctttac gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc
    11221 agcaccacca acacgcacca gacgtacgga ttggacgggt aagcccacag cagcacggag
    11281 atcacaatcg ctgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag
    11341 tgtgattcag gaccgtcgtt acgcactacc tgaccaaagg aaagtttttg caaatgagca
    11401 atcatgcgcg ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg
    11461 cgaaacgtca gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc
    11521 agccaaacta acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcagcac
    11581 tacgtgaacc cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac
    11641 gcgtaattaa cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag
    11701 catggctttg tttacccatg cttaacacgc ggtcaatacc agcagctttt gccgcctcac
    11761 ccacctgtac atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca
    11821 gcacgcggta gcccggcatt tcagccagta cctgaacagc tgcggtcatt gaaccgacat
    11881 tggcgttgta ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac
    11941 ggccaggaac agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca
    12001 cggacatgga gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg
    12061 gcagcagaac atcgacgcta ccggttgggg tttgtagggt aaattccata ccgtgcgagg
    12121 tcacatggat attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca
    12181 ctttgcgtga gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa
    12241 tggcgatacc gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg
    12301 caagcgagcc aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gccgcttccg
    12361 ggcgagtcag actcacagtc caggcaatct cgccctgatg gttcgcgcca agttcaataa
    12421 ctgcgtaatc gtattccggc gttaagcgca acagcgtcat cggtacaccg atgtcgttgt
    12481 tgagattgcc tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct
    12541 ctttaacgga ggttttgccg gaggaccccg tcagagcaac cacgagcgcc ggaacttgct
    12601 ggcgaaccca tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg
    12661 gcaggtcgat gtccagcgga cggctaacca gtagtgcgcc tgcgccgcca gctttcgcct
    12721 ggtcggcaaa atcgtgggca tcaaaacgtt cacctttcag ggcaacaaac aggcagcccg
    12781 gcgtcagttt tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt
    12841 caccgttgag aatatcggta agttggctaa gggttacgct aatcatgcaa tcccccccag
    12901 cagacgcgcc accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta
    12961 atcctcatgg cctttgcccg cgaccagtac cacatcattc tctttagcct gcataacggc
    13021 gcaagtcacc gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc
    13081 cgccagaata tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac
    13141 caccgccacg tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc
    13201 gcgatcgcca ccacagccaa agacgcacca cagcttgccc gcacagtgca ggcgcgccgc
    13261 ctgtaaggct ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt
    13321 gcctggcgca gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt
    13381 cagcagatca gccagtggat agcccagcgc cagcagtgtt gccagcgcga gcaacaggtt
    13441 gctgacgtta aaagcgccca tcagatggct ttcaatttcg ccatcgcccc agcttgaact
    13501 aaagcgaatg gtcgcgccgc tgtcgtgata gttcacttcg gtcgctttca accagcgtcc
    13561 gtgacagttc ggattgatat gatcttccat tgataccgca accgcgtccg gcagttttgc
    13621 cagccagcgg cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc
    13681 agaataaagc agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg
    13741 atcgcggctt aagttggtaa agaccgacgc cggaaatttc aatgccgcca cacggtgctg
    13801 taccagcccg tgggaggaaa cttccattgc gcaaaaagtc gcgccctgat ccaccagccc
    13861 cgccagctca tgctgaacat caactgccga accggtagta ttttctgtcg ggatcacttt
    13921 ccccagcaga ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct
    13981 ccactgcgcc agaagctggg tcgtcgtggt tttgccgttg gtgcccgtta cgcctacgag
    14041 acgtaaattg tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt
    14101 gagctggctg agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc
    14161 ctcatctttc gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata
    14221 tcgacgcccg tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac
    14281 acggctgtcg agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc
    14341 aagaaggtcg cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt
    14401 catttttatc gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc
    14461 ccatgatggc accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg
    14521 gatcgttgat aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg
    14581 tataagcaat atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg
    14641 ttttaatggc gatacgatag cctttaatcg ccgccttcac gccgccgccg cctggtagcg
    14701 ccacgctttc catcatatgc accacggtgc gaacaatgga ttccgggaag acacgttcac
    14761 cgggaaccgg ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga
    14821 tagttgcgta gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga
    14881 aggtggccct ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc
    14941 cgaccaaccc caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg
    15001 ctgaggacgg catcgttaac gccagcttgg aaacaccgac gttactcgac ttctgtaata
    15061 ccccggtcag ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc
    15121 gataaggaat ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca
    15181 tcaccaccat cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac
    15241 gcatcgcctc tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca
    15301 tcgccagcac ttcacctgtg ttgacatcca ccagcacggc gctaccagat tcagccttgt
    15361 taaaggccac cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac
    15421 tcagcgccag gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac
    15481 catagcggtc tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac
    15541 tcttctcaac gccctcaatc ccttgactat cgacgttagt aaagccgatg aggtgagcag
    15601 tcacttcgcc ggacggatag taacggcgag actcttcacg cagatgaatc cccggcagtt
    15661 tcagtttttt gatgtagtcc gccatgtcag agtttacctg acgcgccaga taaataaagc
    15721 gccctttcgg gttggcgtta atgcgggcag aaagctgatc cagcggaata ttgagcgcgt
    15781 tagccagcgc cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg
    15841 ggtcagccca aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag
    15901 taatcatgcc gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt
    15961 tcaccagcat atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca
    16021 gcgccaggag aatacagccg cataacaacg caaaacgcca actgataaag ttggcatgtt
    16081 cttcctgacg ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt
    16141 atttttgcac tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg
    16201 tggcgatcct ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc
    16261 gccattcaat gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta
    16321 aacgggtatg gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca
    16381 ggcagagtgg cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat
    16441 ggcgctcgtg gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga
    16501 tcatgcgttc gtcctctctg caatacgcag aactgaacta cgggcacgag ggttctcagc
    16561 cacctcttct tcgcccggca ttaacttgcc tagtgctcgc agctgacggc cacccagttt
    16621 tttgagctgc tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc
    16681 acgcataaaa cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg
    16741 cccacccggg gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc
    16801 actgtttacc caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc
    16861 tttcaccggc gttgcagcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg
    16921 ttcgcggtta cgctcgacaa tggcgcgggc aatgcgtttg gcaaaatgct cttcaccata
    16981 ggttttcaat acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga
    17041 ctgcccacgg gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc
    17101 gcgttcagca tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat
    17161 cttgccaata agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat
    17221 gatggagaag cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc
    17281 gatcgccagc aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc
    17341 gcgaccaaaa gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc
    17401 ttcatccagc agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct
    17461 gcagtcgctc cgataagtct ccggtagcca actgctctgc gtcgatatct tccttgacct
    17521 gttgatgcca ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca
    17581 cttcttttgt cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc
    17641 catccatctg acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaaccg
    17701 ggttcatgct caacagacgc gataattttt gctcgataat ttcccattca ggcagggggt
    17761 aaagcagcag gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct
    17821 caagcagctg ttcccgataa cgggtaggca ctgataagcg ccctttgctg tcgagattga
    17881 ctaacgttgc tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt
    17941 tcccactgaa aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata
    18001 ccgaggtaaa aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg
    18061 tgcgaagcaa attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcactgt
    18121 tttaaaatga ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg
    18181 cattttagga catatcttcc ccggttaaca gtccttgtta cgtctgtgtg gcgcttgatg
    18241 ccagcgggtt tgcgcaaaag caaagaaaag tgtttgttaa atcggcaatt catcgccgca
    18301 tgtaacaaaa taatgcaaaa acgcgctgtc gatatcactt aattattgtg aatttcagct
    18361 ggtgagttaa ttaacgggcg ggtttaaaac catttatcaa taatagtcag gaatagtctt
    18421 atttacttta aggatattga t
//
LOCUS       DAJTUP010000081        17770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTUP010000081 DAJTUP010000000
VERSION     DAJTUP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.081"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..482
                     /locus_tag="OO468_002839"
     CDS             <1..482
                     /locus_tag="OO468_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6259889.1"
                     /translation="MSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSINMPTVPT
                     TVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTV
                     GDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
     gene            563..1237
                     /locus_tag="OO468_002840"
     CDS             563..1237
                     /locus_tag="OO468_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6259890.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1253..3715
                     /locus_tag="OO468_002841"
     CDS             1253..3715
                     /locus_tag="OO468_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6259891.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGATVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3731..4764
                     /locus_tag="OO468_002842"
                     /pseudo
     CDS             3731..4764
                     /locus_tag="OO468_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4846..5184)
                     /gene="rcnB"
                     /locus_tag="OO468_002843"
     CDS             complement(4846..5184)
                     /gene="rcnB"
                     /locus_tag="OO468_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR6259892.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5403..6233)
                     /gene="rcnA"
                     /locus_tag="OO468_002844"
                     /pseudo
     CDS             complement(5403..6233)
                     /gene="rcnA"
                     /locus_tag="OO468_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6355..6627
                     /gene="rcnR"
                     /locus_tag="OO468_002845"
     CDS             6355..6627
                     /gene="rcnR"
                     /locus_tag="OO468_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR6259893.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6719..6815
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6850..7638
                     /gene="thiM"
                     /locus_tag="OO468_002846"
     CDS             6850..7638
                     /gene="thiM"
                     /locus_tag="OO468_002846"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR6259894.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7635..8435
                     /gene="thiD"
                     /locus_tag="OO468_002847"
     CDS             7635..8435
                     /gene="thiD"
                     /locus_tag="OO468_002847"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR6259895.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8500..9321
                     /locus_tag="OO468_002848"
     CDS             8500..9321
                     /locus_tag="OO468_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR6259896.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9369..10115
                     /locus_tag="OO468_002849"
     CDS             9369..10115
                     /locus_tag="OO468_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6259897.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10089..11054)
                     /locus_tag="OO468_002850"
     CDS             complement(10089..11054)
                     /locus_tag="OO468_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR6259898.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11051..12054)
                     /locus_tag="OO468_002851"
                     /pseudo
     CDS             complement(11051..12054)
                     /locus_tag="OO468_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12094..13146
                     /gene="fbaB"
                     /locus_tag="OO468_002852"
     CDS             12094..13146
                     /gene="fbaB"
                     /locus_tag="OO468_002852"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR6259899.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13454..14308
                     /gene="gatY"
                     /locus_tag="OO468_002853"
     CDS             13454..14308
                     /gene="gatY"
                     /locus_tag="OO468_002853"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR6259900.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14337..15599
                     /gene="gatZ"
                     /locus_tag="OO468_002854"
     CDS             14337..15599
                     /gene="gatZ"
                     /locus_tag="OO468_002854"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR6259901.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15609..16061
                     /gene="gatA"
                     /locus_tag="OO468_002855"
     CDS             15609..16061
                     /gene="gatA"
                     /locus_tag="OO468_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR6259902.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16092..16376
                     /gene="gatB"
                     /locus_tag="OO468_002856"
     CDS             16092..16376
                     /gene="gatB"
                     /locus_tag="OO468_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR6259903.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16380..17735
                     /locus_tag="OO468_002857"
     CDS             16380..17735
                     /locus_tag="OO468_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR6259904.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4625 a         4076 c         4536 g         4533 t
ORIGIN      
        1 ttatgagcgc tggagtattt gctgcagacg ttgataccgg aacattaacc attaaaggaa
       61 atattgcaga atctccgtgt aaattcgaag cgggtggtga ttcagtaagt attaatatgc
      121 cgactgtacc aaccactgtc tttgaaggta aagctaaata ttctacctat gatgatgcag
      181 tcggtgtaac cagcagcatg ttaaaaatta gctgcccgaa agaagttgct ggtgtaaaac
      241 tctcgttgat cacaaacgac aaaataaccg gtaacgataa ggcgatagcc agtagcaacg
      301 ataccgtagg tgataacagc gatgtcctag atgtttctgc accttttaac attgagagtt
      361 ataaaacagc ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag
      421 ataactcagt gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt
      481 aataaataaa attgcaagtt tctccaggta ataaacatgg ccgctatccc catggcggct
      541 ttttaattta agaggcatta agatgaaagg attattatct ttactcattt tttctatggt
      601 ccttcctgca catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa
      661 taaagaagtt atggtgcagt tgatgaacca gggaaatcgt tcttcgctgc tgcaggcgtg
      721 gattgatgat ggcgatacgt cgttaccacc agaaaaaatt caggttcctt tcatgttaac
      781 gccaccagtg gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc
      841 gaataaactg cccactaata aagaaagcat tttttatctg aatgttctgt atattccacc
      901 aaatagtcca gagcaagaag gcaagaatgc actgaagttt gcgatgcaaa acagaattaa
      961 gttgttttac cggccagcgg gtattgctcc ggtaaataaa gcgaccttta aaaagttact
     1021 ggtaaatcgc agtggcaatg gattggtgat aaaaaatgac tcagctaatt gggtgacgat
     1081 ttcggatgtc aaagctaata atgttaaagt caattatgaa actattatga ttgccccctt
     1141 agaaagtcag agtgttaata tcaaaagtaa taatgcaaat aactggtatc tgaccattat
     1201 caatgaccac ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag
     1261 aatgacccca cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc
     1321 tgaagaaacc tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa
     1381 tattcgtctt gatgataatc aacccttacc ggggcagtat gacatcgata tttatgtcaa
     1441 taagcaatgg cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt
     1501 atcaagagaa gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca
     1561 atgtttaaca tttgagcaac ttgttcaggg tgggagctat agctgggata tcggggtttt
     1621 tcgtctcgat ttcagtgtcc cgcaggcttg ggtggaagaa ctggaaagtg gctatgttcc
     1681 accggaaaac tgggagcggg gtattaatgc gttttatact tcttattatg tgagtcagta
     1741 ttacagcgac tataaagcgt cgggtaacaa caagagtaca tatgtacgtt ttaacagcgg
     1801 gttaaattta ctggagtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa
     1861 tccaggggtg tggaaaagca ataccctgta tctggaacgt ggatttgccc aatttctcgg
     1921 cacgcttcgc gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag
     1981 cggtgtgcgg ttgtttcgtg atatgcagat gttgcctaac tcgaaacaaa attttacgcc
     2041 acgggtgcag gggattgctc agagtaacgc gctggtaact attgaacaga atggttttgt
     2101 ggtttatcag aaagaggttc ctcctggccc gtttgcgatt acagatttgc agttggccgg
     2161 tggtggagca gatcttgatg ttagcgtgaa agaggcggac ggctcggtaa ccacctatct
     2221 ggtgccttat gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat atgattttgc
     2281 ggcgggggcg agcaagcaaa gtgattttgt ccaggcaggt tatcagtatg gttttaataa
     2341 cttattgacg ctgtatggcg gcacgatggt tgctaataat tactatgcgt ttaccctcgg
     2401 aacgggttgg aacacgcgca ttggcgcaat ttccgtcgat gccacgaagt cgcatagtaa
     2461 acaagacaac ggcgatgtgt ttgacgggca aagttatcaa attgcctaca acaaatttgt
     2521 gagccaaacg tcgacgcgtt ttggtctggc ggcctggcgt tattcgtcgc gtgattaccg
     2581 gacatttaac gatcacgttt gggcaaacaa taaagataat tatcgccgtg atgaaaacga
     2641 tatctatgac attgccgatt attaccagaa cgattttggc cgtaaaaata gcttttctgc
     2701 caatatgagc cagtcattgc cagaaggctg gggttctgtg tccttaagta cgttatggcg
     2761 agattactgg gggcgtagcg gcagcagtaa ggattatcag ttgagttatt ccaataactg
     2821 gcggcggata agctataccc tcgcggcaag ccaggcgtat ggcgagaatc atcatgaaga
     2881 gaaacgtttt aatattttta tatcaattcc ctgtgattgg ggtgatgacg ttacgacgcc
     2941 tcgtcggcaa atatatatgt ctaactcaac gacgtttgat gatcagggtt ttgcctcaaa
     3001 taatacggga ttatcaggaa cagtagggag tcgggatcag ttcaattatg gtgtcaacct
     3061 gagtcatcaa catcagggaa atgaaacgac agctggggcg aatttgacct ggaacgcgcc
     3121 ggttgcgaca gtgaatggca gttatagtca gtcgagtact tatcgacaga ctggagccac
     3181 tgtttcaggg ggcattgtcg cctggtcggg tggcgttaat ctggcgaacc gtctttccga
     3241 aacgtttgct gtgatgaatg cgccaggaat taaagatgct tatgtcaatg ggcaaaaata
     3301 tcgcacaaca aaccgtaatg gagtggtggt atacgacgga atgacacctt atcgggaaaa
     3361 tcacctgatg ctggatgtgt cgcaaagcga tagcgaagca gaattacgtg gcaaccggaa
     3421 aattgccgcc ccttatcgcg gcgcggttgt actggttaat tttgataccg atcagcgcaa
     3481 gccctggttt ataaaagcgt taagagcgga tggtcaacca ttaacgtttg gttatgaagt
     3541 caatgatatc catggtcata atattggcgt tgtcggccag ggaagccagt tatttattcg
     3601 caccaatgaa ataccgccat cggttaatgt agcaattgat aagcaacaag gactttcatg
     3661 cacaatcacc ttcggtaaag agattgatga aagtagaaat tatatttgcc agtaatagtc
     3721 aggtggttct atggaaattc gcataatgct atttatatta atgatgatgg ttatgcctgt
     3781 gagctatgcg gcatgttata gtgagttatc tgttcagcac aatttggttg ttcaggggaa
     3841 ttttgcactt actcaaacac aaatagcgac atatgagcat aattttaatg attcgtcatg
     3901 cgtgagtaca aatactatca cccctatgag cccatcggat attattgttg gactttataa
     3961 cgataccatt aaattaaatt tacattttga atggaccaat aaaaacaaca tcacgttgtc
     4021 aaataatcag agcagtttca ccagtggtta ttcggtaacg gtgacacctg cggccagtaa
     4081 tgcaaaagtg aatatttctg cggggggtgg tggttcagtg atgattaatg gtgttgcgac
     4141 attatccagt gcttcatcat cgacacgcgg gagtgccgca gtacaatttc tactgtgttt
     4201 attaggtggc aagtcatggg gtgcatgtgt aaatagctac agaaatgcat tggcacaaaa
     4261 tgcaggtgtc tattccttca atctgacatt gtcatacaac ccgataacca caacctgcaa
     4321 acaggacgat ttattaatta ctttagacag tattcccgtt ttacaattac cagccacagg
     4381 taacaaagca acaataaata gtaaaaaaag ggatattatt ctgcgttgta aaaatttatt
     4441 aggtcaacaa aatcaaacat cacggaaaat gcaggtgtat ttatcaagtt ctgacttgtt
     4501 aaccaacagc aacacaatac tgaaaggtgc ggaagataat gcgtaggatt tattcttgaa
     4561 agtaatggtt cgccagtcac acttttaaat atcactaaca gcagtaaagg atacacaaat
     4621 ttaaaagaaa ttgcggcaaa gtcaaaactt acagatacaa cggtttcaat tccgataaca
     4681 gccagttact acgtctacga tacaaacaaa gttaaatctg gcgcactgga ggcaaccgca
     4741 ttaatcaacg tgaaatacga ctaataacaa gcaaaacgag caagtggcta ataataaaaa
     4801 ataacaaggt gtctgcattt ccccctcatg atgagggggc tttttttagc gatgataaaa
     4861 aatctcaccg tcgtaggctt taatgatttt accgtcggtg tcgctgatca acacgtacgc
     4921 gccacccata taggtccagt gcgtcccggc atcaggcgcg ggcaggttac gtaactgcca
     4981 ctgcttaatg ttgtactcat cggtgcggta catctctggg acggtatcac ctggtttgaa
     5041 atgcttaaag tcagcaatga aactacttaa ctcatactta gaaattcccg aggtattggt
     5101 cgaacccgcg gtggctggtg cggcccaggc ggcactggta accagcaaaa gcacacccag
     5161 caacatctta tttttaatgg tcataaactc tccactttgg ggatctgtaa tatcagtcgc
     5221 atatctttcc tgtcatacaa tataactgac aagcgatatt ttgttagtga tcccatgatt
     5281 tcgtaaaaag tgtaaatgaa aggatgccct aaaagcatcc ttagcgagca gagagtcagc
     5341 ggaaatagct gatttaagaa tcatcctggg ctgaagataa catgcaagcc taaaggattg
     5401 agttatcgca ttatgcccat gaagccatgt acgcccatat acacaccgac taagccaatc
     5461 aacagactgg aaaaataggg ggcgcgttta gcgagagtgt taaatccgct ccagcgtttt
     5521 gcgacctgct gaacgctgat tgctgcgcca acgcctacgg tgacaagcgt taacgccagg
     5581 ccaatgctga aactgacgac cagtgttgcg cccagtgtca gggctttcaa ctgaatgcat
     5641 atcaacagca cggtaattgc tgccgggcag gggataaagc caccggttaa gccaaataac
     5701 aaaatttgcc agttgatgac ctctctacca tcaaagcgtc gtttaatgtc attggcatgg
     5761 gctcgtgcat gggcatcctg atactcgcca tgttcgtgat ggtgatgatg tccttggtcg
     5821 tggtgatgtt cgtgatcgtg atgatggtga tgatgctcat aatcatgccc gtgcatattc
     5881 tccagccagt tgcgttcgcc gcgctaggta cgccagaaca tccagaacgc ggtgccaata
     5941 atgatcactg cggaaatcag ctggagccac ggttctgctg attgagcagt aaagcgctta
     6001 ctgatcacca tcccgccaaa ggcaatcaac cagaccaccg cggtatgcga aatggttgct
     6061 gccagtccga gcatcaccgc ctgtttaatg gtgcctttga tggcgatgat aaacgccgcc
     6121 atcatcgttt ttgagtgccc tggttccagg ccatgaagcg caccaagtaa gatggcgctg
     6181 gggatgaaga accaggcgtt tccttgctga agaagagttg taaattcggt catgataata
     6241 attcttagta ttaattcggc aatctgattc tactcccccc cagtacctga tactaccccc
     6301 ccagtagatt aatagtgcta tgatttttca tattcttgtt aaccaggtgt tgccatgtct
     6361 catacaatcc gtgataaaca gaaactgaaa gcgcgtgcca gtaagattca gggccaggtc
     6421 gtggcgctca agaaaatgct cgacgagccg cacgaatgcg ctgcagtttt acaacagatt
     6481 gctgctatcc gtggagcggt aaacggtctg atgcgggaag tgattaaagg tcatctgacg
     6541 gaacacatcg ttcaccaggg ggatgagcta aaacgtgaag aagatctgga tgtcgttctg
     6601 aaggtgctgg attcatatat caaataattt attaacgcga ttgtaaaact gccgtttttc
     6661 ctcgtttaca acgggggcgc tggacattac catcctcctc tgcgatttat catcgcaacc
     6721 aaacgactcg gggtgccctt ctgcgtgaag gctgagaaat acccgtatca cctgatctgg
     6781 ataatgccag cgtagggaag tcacggacca ccaggtcatt gcttcttcac gttatggcag
     6841 gagcaaacta tgcaagtcga cctgctgagt tcagcgcaat ctgcgcacgc gttacacctt
     6901 tttcaccaac attcccctct tgtgcactgc atgaccaatg atgtggtgca aacctttacc
     6961 gccaatacct tgctggcgct cggtgcatcg ccagcgatgg ttatcgaaac cgaagaggcc
     7021 agtcagtttg cggctatcgc cagtgccttg ttgattaacg ttggcacact gacgcagcca
     7081 cgcgcacagg cgatgcgtgc tgccgttgag caagcaaaaa gctctcaaac acgctggacg
     7141 cttgatccag tagcggttgg tgcgctcgat tatcgccgcc atttttgtca tgaactttta
     7201 tcttttaaac cggcagcgat acgtggtaat gcttcggaaa tcatggcatt agctggcatt
     7261 gctaatggcg gacggggagt ggataccact gacgccgcag ctaacgcgat acccgctgca
     7321 caaacactgg cacgggaaac tggcgcaatc gtcgtggtca ctggcgaggt ggattatgtt
     7381 accgatggac atcgtatcat tggtattcac ggtggcgatc cgttaatgac caaagtggta
     7441 ggaactggct gtgcattatc ggcggttgtc gctgcctgct gtgcgttacc aggcgatacg
     7501 ctggaaaatg tcgcatctgc ctgtcactgg atgaaacaag ccggagaacg cgcagtcgcc
     7561 agaagcgagg ggccaggcag ttttgttcca catttccttg atgcgctctg gcaattgacg
     7621 caggaggtgc aggcatgaaa cgaattaacg ctctgacgat tgccggtact gatccgagtg
     7681 gtggtgcggg gattcaggcc gatcttaaaa ccttctcggc acttggcgct tatggttgct
     7741 cagttattac tgcactggtg gcgcaaaata cccgtggcgt acagtcggtg tatcgcattg
     7801 agcctgattt tgtcgccgcc cagctcgatt cggtgttcag cgatgtgcga atcgatacca
     7861 ctaaaatcgg tatgttggcg gaaaccgata ttgttgaagc ggtggcagaa cggttgcaac
     7921 gttatcagat ccaaaacgtg gtactcgaca ccgttatgct ggcaaaaagc ggcgacccgc
     7981 tgctttcacc ttcggcggtt gctacgctgc gcagtcgatt attgccacag gtttcattaa
     8041 taacgccaaa cttgcccgaa gctgccgcct tgctcgacgc gccacacgcg cgcaccgaac
     8101 aggaaatgct ggaacaaggg cgatcgctgt tggcgatggg ctgtggcgca gtgctaatga
     8161 aaggtggtca tctggatgat gagcaaagcc cggactggct gtttacccgc gagggtgaac
     8221 aacggtttac cgcaccgcgc attatgacca aaaacaccca cggcactggt tgtacactct
     8281 ctgcggcgtt ggctgcacta cgcccgcgcc atacaaactg ggctgacacc gtacaggagg
     8341 caaaaatctg gctttcatcg gcgttagccc aggccgacac gctggaagtt ggtcacggta
     8401 ttggtccggt tcaccacttc cacgcctggt ggtgagcacc gtactatagc tgctttggtt
     8461 attgtcagcc tttggcactg acattcaggg atgaaaccaa tgcagctaag aattaccagt
     8521 cgaaaaaaac tcaccgctct attatgcgct cttgggctaa tttccatcgt tgcaatttat
     8581 ccgcgtcaga cggtgaattt tttctattcg acagcagttc agattacaga ctacatccac
     8641 ttctacggtt atcgcccggt taaatctttc gctattcgca ttcctgccag ttacaccatt
     8701 cacggattag atgtttcgcg ctggcaggag cgtatcgact ggcagcgggt ggcaaaaatg
     8761 cgcgacaacg atatccgctt acagtttgct tttattaagg ctacggaagg cgaaaagctg
     8821 gtggacccct atttttcgcg taactggcaa ttaagccgcg aaaatggcct gctgcgcggg
     8881 gcgtatcatt atttctcccc gtcggtatcc gcttcagttc aggcgcgatt atttctgcaa
     8941 acggtggatt tctcacaagg cgatttccct gccgtgctgg acgtagaaga acggggaaaa
     9001 ttatcggcaa aagaattacg caagcgggta agtcagtggc taaaaatggt cgaaaaacgc
     9061 acgggaaaaa agccgattat ttactcagga gccgtttttt atcacaccaa tctggcgggc
     9121 tatttcaatg aatatccgtg gtgggtggct cactattatc aacgtcgtcc ggacaatgac
     9181 ggcatggcct ggcgcttctg gcagcattcc gaccgtggac aggtagatgg catcaatggt
     9241 ccggtggatt ttaatgtgtt taatggcacg gggatgagtt gcaggcattc gttgatggga
     9301 ttaaagaaac gccttaaata agaagcagtg atatactccc accagactcc ctggcaggaa
     9361 aaagacaaat ggaacaagcg catacccagc tgattgccca actgaacgag cgtattttag
     9421 cggcggataa cacgccgctt tatattaagt ttgccgaaac ggtaaaaaat gccgtgcgca
     9481 gcggggtgct ggagcatggc aatattttgc ccggtgagcg tgatttaagt cagttaaccg
     9541 gcgtttcgcg cattacggtg cgcaaggcga tgcaggcgct ggaagaagag ggcgtggtga
     9601 cgcgttcgcg cggttacggc acgcagatca acaacatctt cgaatactcg ttgaaagaag
     9661 cgagaggttt ttctcagcag gtggtattgc gcgggaaaaa gcccgatacg ctatgggtta
     9721 ataagcgtgt cgtgaaatgt cccgaagaag ttgcgcagca gctggcggcc gaagcgggaa
     9781 gtgatgtctt tttgcttaag cgtattcgct atgtcgatga agaagcggta tcgattgagg
     9841 aatcgtgggt tccggcgcat ttaattcatg atgttgatgc catcggaatt tctctttatg
     9901 actatttccg cagccagcac atttacccac aacgtacgcg ttcccgcgtt agcgcccgga
     9961 tgccggatgc cgagtttcag tcacatattc agttagatag caaaataccg gtgctggtga
    10021 tcaagcaagt ggcgcttgac caacagcaac ggcctattga gtacagcatt agccactgtc
    10081 gcagcgatct atacgttttt gtgtgcgagg agtagttcct cgcgcgttgg cgcacaatca
    10141 ccgccccgat gcccgacaac ccacgacgcc actgcattgc ccagtaatac ggcatccgcc
    10201 agtggcagac cagaggccag cccggcaagt acgccacctg catgactgtc gcccgcccca
    10261 atggtgtcta caacttgcgt tggaaatgcc ggaatgcagc cagaagcgtc gttgctgaaa
    10321 taccatgcgc cttctttatc gagacgaacg atcaacggcg cggcaaattt ctcctgccat
    10381 tgcttgccaa gtgttgttat ctctgcggat aaagcaaaac gttcggcggc aatctcagcc
    10441 tcttgacgat tgagtgacac taaaggtcga caggccatga tccgcgccag taatgcgtcc
    10501 gggatatcgc caatacgtgg gcaaaaatcg ataaacggcg tcacgtcttg cagcttttcc
    10561 agccattcca ctaacaattc gccgcagggc gaggccagtt gataaccgga aaaatagagc
    10621 aggttgccag gcgcaacggt taatcgtgcc aaccattggc gattccattg attttccaca
    10681 ccgctgaatg acataaaagt gcgttcgcca tccggctcaa ccagtgccag acaccagccg
    10741 ttatcgcttt cggcgttatc gatcaggctg attaacccct ctttcgccat ccggttgcga
    10801 ataatctccg cccacacgcc ctgaccgagc ggcaaggcat tacccgcttc gatgccgagg
    10861 cgctttaacg ccacggcaat attcagtgcg cagccgccaa cgttaacgct ctgctgtttc
    10921 agttcgatat cgcacccacg ccaggggagg gcataagcgt cggcgatcac gtcaatgacc
    10981 gccgcgccga cgaccatcac cggctgacgc gtggttaatt caggcagcag tgtgtgtaat
    11041 cgagcgccgc tcataccgcc tcccgttgtt gacgatattt cgccagcgct gtggcatagc
    11101 ggttgaaatc aagctgattt accgcatcca gctccgcctt taacgcagga tcgacagcgt
    11161 taatgccatg caacgcgccg caaatcgccg tcgccatagc acctatggtg tctgtgtcgc
    11221 cgccaagatt ggcgcacagg acggcgcagc ggtttgggtc ggtttgtgcc agttcaacta
    11281 gcgcaatggc gcacggaacg gactcaatag tgctggtgcc tgcgccaacg acctggtaaa
    11341 gctgttcgct ggcggattcg gtgccgtcgg cattacgcac aattttcagc gcaatctcca
    11401 gacgtgccgc cagtgaggcg ctgaacgtgg tggtgcgttt ttgctgtgca tgtcgcgcaa
    11461 ttgaaggcaa tgaatcaaca atcgctgacc agcgttctcc gtcaatggca cgagaaatcg
    11521 cccatgcgat gactaccgcg cctgcaaccg ccagatcgga tttatgggtc gggctggacg
    11581 ccagcgccac atcatcaata aaggaatcaa catcacgcgc tggaagcaaa caacctaatg
    11641 gcgagacgcg catcgctgcg ccgttggtta cgccgttgtt ttccagttca gcaacgggtt
    11701 taccgtcgcg aatggcgtta agcgcaatct tcgaggtcgg acctagtacg tttttgttaa
    11761 aggcgtcgaa acgcaacgcc cagtcgagaa tattacgccc aatcagattc ggatcgatct
    11821 tgccttcaca ttccagtaac gcatccgcca gacacaggcc atcgaggtat cgtcggtgaa
    11881 ttcggcgcgg ttaaaataac aggccgcgtt attctccttt ggtccaggca gaaaacggtc
    11941 aatccagcca aagtgtgctt taacgcggct gcgtggccaa agctcggagg gcatccccat
    12001 cgcatcccct aacgcctgcc cataaagagc accgagaata cgttctgttt tcatttaact
    12061 tccccttgaa tcggaattta cgggagcata gtaatgacag atattgcgca gttgcttggc
    12121 aaagacgccg acaacctttt acagcaccgt tgtatgacta ttccttctga ccagctttat
    12181 ctccccggac atgactacgt agaccgcgtg atgattgaca ataatcgccc gccagcggtg
    12241 ttacgtaata tgcagacgtt gtacaacacc gggcgtctgg ctggcacagg atatctttct
    12301 attctgccgg ttgaccaggg cgttgagcac tctgccggag cttcatttgc tgctaacccg
    12361 ctctactttg acccgaaaaa cattgttgaa ctggcgatcg aagcgggctg taactgtgtg
    12421 gcatcaactt acggcgtgtt ggtgtcggta tcgcggcgct atgcgcatcg cattccattt
    12481 ctcgtcaaac ttaatcacaa cgagacgcta agttacccga atacctacga tcaaacgctg
    12541 tatgccagcg tggagcaggc cttcaacatg ggcgcggtgg cggttggtgc gactatctat
    12601 tttggctcgg aagagtcacg tcgccagatt gaagaaattt ctgcggcctt tgaacgcgcg
    12661 cacgagctgg gcatggtgac agtgctgtgg gcctatttgc gaaactccgc ctttaagaaa
    12721 gatggcgttg attaccatgt ttccgccgac ctgactggtc aggcgaacca tctggcggca
    12781 accataggtg cagatatcgt caaacaaaaa atggcggaaa ataacggcgg ctataaagcg
    12841 attaattacg gttataccga cgatcgcgtg tacagcaagt taaccagcga aaacccgatt
    12901 gatctggtgc gttatcagtt agctaactgc tatatgggcc gggctgggtt gataaactcc
    12961 ggcggtgctg caggcggtga aactgacctc agcgatgcag tgcgtactgc ggttatcaac
    13021 aaacgcgcag gcggaatggg gctgattctt ggacgtaagg cgttcaagaa atcgatggct
    13081 gacggcgtga aactgattaa tgccgtgcag gacgtttatc tcgatagcaa aattactatt
    13141 gcctgatacg cctcatacga acagcaccac cccgtaggac ggataagacg tttacactgc
    13201 atccggcaac ctatgcctga tgcgacgcgg aagcgtctta tcatgcctac atagcactgc
    13261 cacgtatgtt tacaccgcat ccggcataaa aacacgcgca ctttgctacg gcttccctat
    13321 cgggaggccg tttttttgcc tttcactcct cgaacaattt tcatattgtt gtttttgcga
    13381 tcattatctc gacatttaaa agcaaataat ttcattatat tttgaaatcg aaagcaaacg
    13441 acaagatatg aaaatgtacg tggtatcgac aaagcagatg ctgaacaacg cacagcgcgg
    13501 cggttatgcg gttccggcat tcaatattca caatctcgaa acgatgcaag tggtggtgga
    13561 aaccgctgcc aacctgcatg cgccggtcat catcgccgga acgcctggca catttactca
    13621 tgctggtaca gaaaatctgt tggcgctggt cagcgcgatg gcgaagcaat atcaccatcc
    13681 actggcaatt catctcgacc atcacacgaa atttgacgat atcgctcaga aggttcgttc
    13741 tggcgtgcgc tcagtcatga ttgacgcctc gcatttgcct tttgcgcaaa atatttcacg
    13801 ggtcaaagag gtggtggatt tttgccatcg ctttgatgtc agcgtcgagg cggagctggg
    13861 gcaacttggc ggccaggaag atgatgtgca agtcaatgaa gtcgatgcgt tgtacaccaa
    13921 ccccgcacag gcgcgtgaat ttgccgaggc aaccgggatt gattccctgg cggtcgccat
    13981 cggcacggct cacggtatgt atgccagcgc accggtgctt gatttttctc gactggagaa
    14041 cattcgccag tgggtgaact taccgctggt gctgcatggc gcgtcaggat tatcgactaa
    14101 ggatattcag caaaccatca aactggggat atgcaaaatc aacgttgcaa cggagctgaa
    14161 aaatgccttc ttgcagtcgt taaaaaatta cctgaccgag caccccgaag cgaccgatcc
    14221 ccgggattat ttgcagtcgg ctaaatccgc aatgcgcgat gtggtgagca aagtgattgc
    14281 cgattgtggc tgcgagggca gggcataacg cacctgccat ttaacaagga aaaaacatga
    14341 aaacgttaat tgcccggcat aaagctggtg aacatatcgg catatgttca gtctgttctg
    14401 cccatccgtt ggttatcgaa gcggcgctgg catttgatcg caacagcacg cgcaaagtgc
    14461 tgattgaagc aacgtcaaac caggtcaatc aatttggcgg ttataccgga atgacaccgg
    14521 cagactttcg cgaatttgtt tttacgattg ccgataaagt tgggtttgca cgcgaacgca
    14581 ttattctcgg cggcgatcat ctgggaccaa actgctggca gcaagaaaat gcggatgcgg
    14641 cgatggaaaa atccgtcgag ctggtaaaag catatgttcg tgctggcttc agtaaaattc
    14701 atcttgatgc gtcaatgtcc tgcgcggggg ataccatacc gttagcacca gaaacggttg
    14761 cggaacgagc tgctgtgctt tgctttgctg cggaaagtgt ggcgacagat tgccagcgtg
    14821 agcaactgag ctatgtcatt ggcactgaag ttccggttcc gggcggtgag gccagcgcca
    14881 ttcagtcagt acacatcacc catgttgaag atgccgccaa tactttacgt acgcatcaaa
    14941 aggcctttat tgcccgtggg ctgacagagg cgttaacacg tgtgattgcc atcgtggtgc
    15001 agccgggtgt ggaatttgat cacagcaata ttatccatta tcagccgcag gaagcgcagg
    15061 cgctggcgca atggatagaa aacacccgaa tggtttatga agcacattct accgattacc
    15121 agacccggac ggcttattgg gaattagtcc gcgatcactt tgcaatattg aaagtcggtc
    15181 ccgcattaac ctttgcttta cgcgaggcga tatttgcact ggcacaaatt gagcaggaac
    15241 ttatcgcccc cgaaaatcgc agcggttgcc tggcggtaat tgaagaagtg atgctcgacg
    15301 aaccgcaata ctggaaaaaa tattatcgca cgggttttaa cgattcatta ctggatattc
    15361 gttacagcct gtcggatcgt attcgttatt actggccgca tagtcggatt aaaaatagcg
    15421 ttgaaacgat gatggtgaat ctgcaaggcg tggacatccc actgggcatg attagtcagt
    15481 atcttcccaa acaatttgaa cgcattcagt ccggggaatt atcagcaatg ccgcatcagc
    15541 ttattatgaa taaaatttat gatgttttgc gcgcctatcg ctacggctgt gcggaataag
    15601 gacggtatat gactaacctg tttgttcgta gcggaatttc ttttgtcgat cgtagcgaag
    15661 ttttaaccta tatcggtaat gagatgctcg cgaaaggtgt ggttcatgat acctggccac
    15721 aggcattaat tgccagagaa gcagaattcc ctaccgggat aatgcttgag cagcacgcca
    15781 ttgcaatacc gcattgtgag gcgattcatg ctaagtcgtc agccatttat ctgttaaggc
    15841 caacaaataa agttcatttt cagcaagcgg atgatgataa cgacgtggcg gtatcgttgg
    15901 ttattgcgtt gattgtggaa aatccgcagc agcaattgaa acttttacgc tgtttatttg
    15961 gcaagttaca acagcccgat atcgtcgaga cactaattac tcttcctgaa actcagttaa
    16021 aggaatactt cacaaagtat gttttagatt cagacgaata aatccctctg taacaataaa
    16081 aaggactatt tatgaaacgc aagattattg tcgcttgcgg aggcgcggtt gcgacctcta
    16141 cgatggcggc ggaagaaatt aaagagttgt gtcagagtca taatattcct gttgaattaa
    16201 tccagtgtcg ggttaatgaa atagaaacct atatggatgg tgtgcatttg atatgtacca
    16261 ctgccagggt ggatcgtagt tttggcgata ttccgttagt tcacggcatg ccttttgttt
    16321 ctggtgtcgg tatcgaagca ttacaaaata aaattctgac tatcttacag ggatgaccta
    16381 tgttttcaga agtcatgcgt tatattctcg acctcggccc tacagtgatg ctgccggttg
    16441 tcatcattat tttttctaaa atattaggca tgaaggcagg cgattgcttt aaagcgggtc
    16501 tgcatatcgg gattggcttt gttggcattg gccttgtgat tggcttaatg ctggattcca
    16561 ttggtccggc ggcgaaagcg atggcggaaa atttcgacct gaatctgcat gtggtcgatg
    16621 tcggctggcc gggctcttca ccaatgacct gggcgtcgca aattgcgctg gtggcgattc
    16681 cgattgcgat attggttaac gtggcgatgt tactgacccg tatgacgcgg gtggtaaatg
    16741 ttgatatctg gaatatctgg catatgacct tcaccggcgc gttgctgcac ctggcaaccg
    16801 gttcatggat gatagggatg gcgggtgtgg taattcacgc ggcgtttgtt tataagctcg
    16861 gcgactggtt tgcccgcgat acccgaaatt tctttgagct ggaaggtatt gctattccgc
    16921 acggtacgtc ggcgtatatg gggccgattg cggtgctggt cgatgctatc atcgagaaaa
    16981 tcccaggcgt taaccgaatt aaatttagcg ccgacgatat tcagcgcaaa tttggccctt
    17041 ttggcgaacc tgtcaccgtg ggttttgtga tggggctgat tatcggcatc ctcgcgggtt
    17101 acgatgtcaa aggtgtattg cagctggcgg taaaaacggc ggcagttatg ctgctgatgc
    17161 cacggatgat taaacccatc atggatggtt taacgcccat cgctaagcag gcccgtagtc
    17221 gtttacaggc gaagttcggc ggtcaggagt tcctgattgg cctggatccc gcattactgc
    17281 tggggcatac ggcggtggta tcggcaagcc tgatttttat tccgctcacc attttaattg
    17341 ctgtttgtgt tccgggtaat caggtactgc cgtttggcga tcttgccact atcggcttct
    17401 tcgtggcgat ggcggtcgcg gtgcatcgtg gaaatctgtt ccgcacctta atctcgggtg
    17461 tcatcattat gagcatcacc ctgtggatcg cgacgcaaac tattggtttg cacacccaac
    17521 cggcggctaa tgctggggcg ttaaaagccg ggggtatggt ggcttcaatg gatcagggcg
    17581 gttctcccat tacctggtta ctgattcagg ttttctcccc gcaaaatatt cccggtttca
    17641 ttattatcgg cgcaatttat ctgaccggta ttttcatgac ctggcgtaga gcgcgtggtt
    17701 ttactaaaca agagaaagcc gttctcgcag aataattttt acctgagggg gaggttatcc
    17761 cccttaacaa
//
LOCUS       DAJTUP010000082        17030 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTUP010000082 DAJTUP010000000
VERSION     DAJTUP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.082"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..326)
                     /locus_tag="OO468_002858"
     CDS             complement(<1..326)
                     /locus_tag="OO468_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR6259905.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPL"
     gene            complement(334..684)
                     /gene="chbA"
                     /locus_tag="OO468_002859"
     CDS             complement(334..684)
                     /gene="chbA"
                     /locus_tag="OO468_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR6259906.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(743..2091)
                     /gene="chbC"
                     /locus_tag="OO468_002860"
                     /pseudo
     CDS             complement(743..2091)
                     /gene="chbC"
                     /locus_tag="OO468_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2176..2496)
                     /gene="chbB"
                     /locus_tag="OO468_002861"
     CDS             complement(2176..2496)
                     /gene="chbB"
                     /locus_tag="OO468_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR6259907.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2795..3133)
                     /gene="osmE"
                     /locus_tag="OO468_002862"
     CDS             complement(2795..3133)
                     /gene="osmE"
                     /locus_tag="OO468_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR6259908.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3335..4162
                     /gene="nadE"
                     /locus_tag="OO468_002863"
     CDS             3335..4162
                     /gene="nadE"
                     /locus_tag="OO468_002863"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR6259909.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4392..5279
                     /gene="cho"
                     /locus_tag="OO468_002864"
     CDS             4392..5279
                     /gene="cho"
                     /locus_tag="OO468_002864"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR6259910.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5239..5814)
                     /gene="ves"
                     /locus_tag="OO468_002865"
     CDS             complement(5239..5814)
                     /gene="ves"
                     /locus_tag="OO468_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR6259911.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6017..6502)
                     /gene="spy"
                     /locus_tag="OO468_002866"
     CDS             complement(6017..6502)
                     /gene="spy"
                     /locus_tag="OO468_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR6259912.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6832..7800)
                     /gene="astE"
                     /locus_tag="OO468_002867"
     CDS             complement(6832..7800)
                     /gene="astE"
                     /locus_tag="OO468_002867"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR6259913.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7793..9126)
                     /gene="astB"
                     /locus_tag="OO468_002868"
                     /pseudo
     CDS             complement(7793..9126)
                     /gene="astB"
                     /locus_tag="OO468_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9123..10601)
                     /gene="astD"
                     /locus_tag="OO468_002869"
     CDS             complement(9123..10601)
                     /gene="astD"
                     /locus_tag="OO468_002869"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR6259914.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10598..11632)
                     /gene="astA"
                     /locus_tag="OO468_002870"
     CDS             complement(10598..11632)
                     /gene="astA"
                     /locus_tag="OO468_002870"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR6259915.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11629..12849)
                     /gene="astC"
                     /locus_tag="OO468_002871"
                     /pseudo
     CDS             complement(11629..12849)
                     /gene="astC"
                     /locus_tag="OO468_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13295..14101
                     /gene="xthA"
                     /locus_tag="OO468_002872"
     CDS             13295..14101
                     /gene="xthA"
                     /locus_tag="OO468_002872"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR6259916.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14268..14978
                     /locus_tag="OO468_002873"
     CDS             14268..14978
                     /locus_tag="OO468_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6259917.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            14983..15660
                     /gene="ydjY"
                     /locus_tag="OO468_002874"
     CDS             14983..15660
                     /gene="ydjY"
                     /locus_tag="OO468_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR6259918.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15675..16382
                     /locus_tag="OO468_002875"
     CDS             15675..16382
                     /locus_tag="OO468_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR6259919.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16382..16930
                     /locus_tag="OO468_002876"
     CDS             16382..16930
                     /locus_tag="OO468_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR6259920.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
BASE COUNT         4199 a         4535 c         4169 g         4127 t
ORIGIN      
        1 aacaacggca ggtaatgctg ctcaaaaaag cgtttactga tcccaacgtt caatatgcgc
       61 gtggcaccaa actcataaaa actttgatgg tgcgaaccta acggaataaa aacaaaatcg
      121 ccccgttcca gtaacacgcg cttaccgtta atctcctgga aataacgccc ggttaatacc
      181 agagtaaatt cataatagtc gtgctgatgc agtccgctga tactctcagt tttgttatag
      241 ataaacacat ggaagttttt gccattaaac aactgctgtt ctcgggcagt ggcaatttcc
      301 ggcgcgttaa tcactggctg catcatcgac tccttatgcc ttcagttttt catgaagctc
      361 aattaattca gtaatcagtt cacgcgcaag catggacgtc attaaatgat cctgagcgtg
      421 gaccagcacc agactcactt tcatcttacc ttcgcccgca tcgccttcaa tcagtttcgt
      481 ctgtaccaga tgcgcttcat tcaatgccat ccgtgactga tccatcatgg ctttcgctgc
      541 ggcaaaatcg ccctgcttcg cctgtttcaa tgcggcatac gccaggctac gcgcttgtcc
      601 agagttgatg ataagcccca tcaccacttc ttccagctct tcagcttctg tttgcgtatc
      661 aggaatatta tcgagatcca tcatacatcg ttcctctctc ttcttaccgg caagattacc
      721 cgtaccggca tcgattaaaa tttcaggggt tagcgatatc ttcttcgctc tcttctttat
      781 caatcgcatt ttgtgctttg ttagccacca caacaaaggg cagataaatt aacgttgcga
      841 tgccaaggtt gaagagtgcg accagcaatg cggcgacgct accgttggtg ttaaagaagg
      901 ctcccagacc ggttggcatg gtccacggtg caatattggt caccggagga atgataccca
      961 tgtagtacgc tgcgagggtg attgccgcca gaatcggttg taccagtaca aacgggataa
     1021 acatcaccgg gttcatgata attggcagac caaacagaat cggttcgtta atctggaaga
     1081 tgccggacgg cagcgccagt tttgccacct gacgataatc agcacggcga gaggcgataa
     1141 agatagccag gatcaggcct aaagtcgcac cactgccccc aaggaaaata aaggagtcca
     1201 gcatcggctt ggcccagata tggaaggtct taccggctgc cagcgccgct tcgacggaac
     1261 catattgctg ataggtcgcg atattttcca gcgcccacgg cgtcataatg ccgttgtcca
     1321 gtgcggtcag cgccagcgcg ccatgaatac cgaagaacca gagcagtgga acaaagatca
     1381 cataggccca gcccaccacg ctacccaacg atgccagtgg ggttgagatg gtatccataa
     1441 tgatctgatg gaagttggtg ccccaggtat tcaacgccca ggcaataatc cccatcacgg
     1501 aaagaataat aaagccggga attaatgccg agaaagaacg cgatactgaa gcaggtacgc
     1561 tgtcgggcag tttaatgacc caattgcggc ggacaataaa ggtaaacatt tctgccacca
     1621 ccaggccaat aataatcccg gagatgatat tcgccccacc taaccagttt gcgccaaccg
     1681 cataggcctc accgacacta tatggggtga cggtcataaa tgctgcaacg gataacaacc
     1741 cagccgccag cgcatcgact ttacgctctt ctgccagcgc catgccaata aagaacggtg
     1801 ccattaaaga cattattcct aatgttccgt tatatacgtt gccgccaata cctttcagac
     1861 cattaagtgt ttcaatggtt gaggcgtcga ggcgaatacc taaggaataa aaaacgaccc
     1921 ctccccaaag cttagaaaaa cgttgttaat taatacaaac atggcccccg caagggttaa
     1981 cggcattaag cgaataaagc catttttgat tgcattaacg tgtggctgct ttcctatttt
     2041 aactgcaaaa gggaggagta ccttttcaag cgatgcaata acattactca tagaaaaata
     2101 cccttaaaaa ccgcaattta aatattacgg tattgattta tgaaataact ctttgacggg
     2161 aaaatttaaa ataaattaat ttgctgcggc ttttttaatc gctgcaaccg cagccttaag
     2221 cacgcctaaa ccatcgactt tgccataaag cagcgagtca attacttcaa ccggtttgtt
     2281 gggtaacaaa cgctggattt cgggcaacat ataagcaatc tgcggcccta ataacacgac
     2341 atcggcattc tgaccttttt caccagccag tgtttccgga aatgcttcaa taatgaccgg
     2401 aacttcatat ttttctgcct gtgcgcgcat ttttgatacc agtaaagaga tagacatgcc
     2461 cgcagaacaa aacagataaa tgtgtttctt ttccataaaa ctgccctcat cgacgattat
     2521 ctgtcagcca gacactccgc aagccttgac ctgcttccat gctctgggta acttgcgaaa
     2581 ccaacatgac tttttttgtg gctgaaagga gtatacggta tagacctgag caatagtatt
     2641 tctttgacct tataaattgt ttctccttga cctgtggtta tgaccctctt cacatttcgg
     2701 gcaaataatt cgcgaagata attaagaact caggccaaaa aaaaccggcg caatggccgg
     2761 tttccgttgt tactcaagct ttcagacgaa ttgattactt cgcagcctgt ggatcagtgt
     2821 cgtattcagc acaggtctga taaccggagt tgatgacatg tccggtatca tctaacgcga
     2881 caaagtaggt ttctgcttta ccatcacgtt gacccaggat gtaggtctgg caagtaccgc
     2941 gagcatggat catgctcact tcagacgaag gtttacccgc aatttgtgca acctgcgccc
     3001 ggctcatgcc ttttttgacg tctttcacca caggctgtac aaactggtct ttggtacgat
     3061 cataagctgt acaacccgcc agcatggtta ataccgccgc tgcactcaga attcctgcca
     3121 tattcttgtt catattccgt cctcttgttt atcagcgtgt tagataagcc tggaatacat
     3181 tgggcgcttt ttcaagcccg tgaacgaaac ggctccgctt tcagaggatt cctgtatgac
     3241 gttttaacca ccattcagcc cgctgtcgct tgtcgtttca gtagcaacgg gttagcttta
     3301 aggaagtttt gtcttttctg tctggagggg ttcaatgaca ttgcaacaac aaataataaa
     3361 ggcgctgggc gcaaaaccgc agattaatgc tgaagaggaa attcgtcgta gtgtcgattt
     3421 tctgaaaagc tacctgcaaa cttatccgtt cattaaatca ctggtgctcg ggatcagcgg
     3481 cggtcaggac tccacgcttg ccggaaagct gtgccagatg gcgattaatg agctgcgcca
     3541 ggaaactcgc aacgaatcac tgcaatttat tgccgtacgc ctgccctatg gtgttcaggc
     3601 tgacgaacaa gattgccagg atgccattgc ctttattcaa ccggatcgcg tattaaccgt
     3661 taatatcaag ggcgcggtat tggctagcga gcaggcattg cgggaagcag gcattgaact
     3721 gagcgatttt gtccgtggca atgaaaaagc gcgtgagcgg atgaaagcac aatatagcat
     3781 tgcgggtatg accagcggtg tcgtggtggg caccgatcat gcagcagaag ccattaccgg
     3841 attcttcact aaatatggtg acggcggtac ggatattaat ccgctgtatc gtctcaacaa
     3901 acgccagggt aaacagttac tgacggcatt aggttgcccg gaacaccttt ataagaaagc
     3961 cccaactgcc gatcttgaag atgatcgccc ttctctgcca gatgaagtgg cactcggcgt
     4021 gacctatgac aatatcgacg actatctgga agggaaaaac ctacctgaac aggtcgccag
     4081 aacaatagag aactggtatc tgaaaaccga acataaacgc cgtccgccaa ttaacgtttt
     4141 cgatgatttc tggaaaaagt aataatttgc acctattgga ttgtgcgaaa aagagtaatt
     4201 tgttcacgcc ggatgcggcg tgaacgcctt attctaccta caaaactatg caaattcaat
     4261 acattgcagg agtcgaatag gcctgacagg cgtagcacgt cagacggtgt aacctttgtc
     4321 atcaacccgc ctctttttta atcgcctccc gcctgttaca ctggatagat aaccagcatt
     4381 cggagtcaac agtggtacgg cgtttaactt ctccgcggct cgaatttgaa gctgcggcaa
     4441 tttatgaata tcccgaacat ttacattcat tccttaatga cttacccacc cgaccagggg
     4501 tgtatctgtt tcatggcgaa agtgacacca tgccgctcta tatcggcaaa agcgttaata
     4561 tccgcagtcg ggtgctttct catttacgca ccccggatga agccgccatg ctacggcaat
     4621 cccggcgaat tagctggatc tgcaccgcag gcgaaatcgg cgctctgctc cttgaagcgc
     4681 gattaatcaa agaacaacag ccgctgttta ataaacggtt gcgccgcaat cgccagctct
     4741 gtgccctgca attaaatgaa aagcgcgtcg atgtggtgta tgccaaagag gtggattttt
     4801 cacgagcccc caacctgttt ggcctgtttg ccaataggcg cgcagctttg caagcattgc
     4861 agagcatcgc tgatgaacaa aaactttgtt atggcctgtt gggactggaa ccgttaagtc
     4921 gcggtcgtgc atgttttcgt tcagcgctaa aacgttgcgc cggagcatgc tgcggtaaag
     4981 agagccatga ggaacatgcg ctacgcttgc gccagtctct ggagcgtttg cgagtggtgt
     5041 gttggccttg gcaaggtgcg gtggcactga aagaacagca cccggaaatg actcaatatc
     5101 atattattca aaactggctg tggctggggg cggttaattc gctggaagaa gcgacaacgt
     5161 taattcggac acccgccggg tttgatcacg acggttataa aattctttgt aagccgctgc
     5221 tttccggtaa ctatgaaatt actgaacttg atccggcgaa tgaccagcga gccagttgat
     5281 ttcactgaac aacagtttac cttgcggttg taaaagccgc agggtgtgcc tgccatcgaa
     5341 ccagcacacg ccctggtcgg tagtgagcag tttgtcgccc agttgccacg ccccgttaat
     5401 cacaaacacc accccgccgc gtgagccaaa ggtagtaaac gtacgttcgg caattctgac
     5461 tttcgcctta cagacatcca ggcgcgtcat aatattgaaa tccatcgaca tctgccctgc
     5521 cgtcagtttc gcttttacca cctggtccgc cgtaaaggca aaaggctgta acggttttaa
     5581 ggtatggtta aagcggtctg cgctttcaag gagcatctcg ccgccttcca gcaacgtcac
     5641 tatcctttcc atgccgggaa acaaagaaaa ctccccattc gccgcgatgg atgcaatgct
     5701 ggcacgccag taaaaatcac gttttgccgg tgggaaagtg caaatttcgc gcgtttcacc
     5761 ggcagcgttt cgccacagat tcaccgacat tttacgcata tcaaagtatt ccatactcgc
     5821 tcccggcccg ttcttttcat tgttatcgtt caggtaaccg tggatggtta tcggcaactc
     5881 gggtgattta cgttagtggt gatcaggctt tttttacaac tgtttgagaa gagaaaagaa
     5941 aaccgccgat cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttaag
     6001 ttttttggct gaaagattat tcagcagttg caggcatttt accttttgcc gctggacgtt
     6061 ctgtcagacg cttctcaaaa ttagcattaa attgcttttt ctgttccggc gtcaggatgt
     6121 tgtaaatttt gttctgggtt tccatgtgcg ccagcatgtt agctttgcgc tgttcttcca
     6181 tttttgcgat ctgcgcttca gcttttgctt tatcgaaggt atcgctggca atgatgtcat
     6241 gcattgcgcg gcgttcttcc agcggcggac gtttcatctg gtcacgctgg cctttcatga
     6301 tttcgtggat ctgctgtttc tgcgcgtcgg tcaggttcag gtctttgaac atcatgtcct
     6361 gatgcggacc gaacttgcct ttgtggtgca tcatcggctt cgcgtcagcc ggtgctgcgg
     6421 tagtggtgtc tgcggcatgg gccaggttag ccgcgccaag agccagggta gaggcaacaa
     6481 acagtgcagt taatttacgc atattctata tccttccttt cagttattta ttacggcttt
     6541 cttaagtagc gtgccgtgtt gacgagatta actttactga ctttagcgtc aattaatcag
     6601 agcaacggta aaacaatgaa agtgtaaaaa acacttttgc gccaattatg gagaaaaaaa
     6661 gaaaatttga tggagagtga tgagagaata ttacaacacg atgattttgc agagattatg
     6721 aagaactata ccggatgact ggtgataaat aaagcaaata accaggatta atctgtatta
     6781 atttataaga aagcaactta atacccgcag aatgatttct gcgggtaagt attagcttat
     6841 tttttcgagc attaatcccg cgcgtaatcc caacgctacc aacggattag ggaataacac
     6901 atactctaca tcatgggtta cggtaaaacg ttcctctccg tcctgcgcca gcaatgttcc
     6961 tttctcaaac ggcataaaat tcagcgtgtc acttgccata tgcatttcga aggacggcga
     7021 gtggcgagta atttgcgaaa ccacccgata acggagcggc ggtgttctca cgataccgac
     7081 actctcacca gatagcagcg cagcaattgc gctggcagtt actgcaaact ggcgaagatc
     7141 gttttgccca aagggcaacg ctttgccaag ttccaacgta caggccagcg cgccaaaatg
     7201 tctgacgctg aaatgggtaa acgtaccacc aggctcctga tggaacacca gcgcctccag
     7261 ccccgccgca cccagccacg tcagaaattt ctcgtcccag ggaatgtcgc gttgcggtaa
     7321 tacaccgaac tgcggatgca aggagccacg aattgcggta tgtagatcaa ggtgccaacg
     7381 cacagattct ttgccctggt cataaaaatc ttccaggcac tgttccagtt cgcgcgcccg
     7441 acaggtttct ccgctttcag caaatagctg ccaacgaccg ccaaacattc ggttcatatc
     7501 gctatggcaa taacgtttcc cttgcttcag cgcaggagga ttcccgagga tcaccagcaa
     7561 ccgccaacgt aacgggatct cgccgtgaga tatcgcgcca agcaacgcgt ccagcatttc
     7621 cacaggtgcc gtctcattac cgtggatccc cgcagaaatc accagcgcgc cttgcggtgg
     7681 cgttaatggc gtcagttcca gcacaccatc gcccagccag cgccagcgaa cgccgttgat
     7741 ttctcgctcg gtgataaccg gttttttacc cgttaaggtc agagcaagaa aattatccat
     7801 tgccccctcc ctcgcgctgg aacggataaa ccgaaccgag attcagtaat tggctcaata
     7861 catccagtgc ttcccgccct tcgcgcagca attgcgggtc ggccagatcg gcagcagtaa
     7921 gacgatcgcg gtagtaacga tccacccagt cattgagcgc attaaacaag gtatcgttca
     7981 tcatcaccgc cggattcacc gcccggcgtt cttcttctgt caataccacc cgcaaccgca
     8041 ggcacgccgg gccgccgcca ttcgccatgc tttcacgtaa gtcaaagact tttagttcgc
     8101 taatcgggtt gtcagcggca aggagttcat tgagataacc ccatactccg gcgtgttccc
     8161 gacactcctg aggcagcacc aacatcatgg aaccatcatc gcggctcaac agttggctgt
     8221 taaacagata ggtagacacc gcatccgaca cggaaacctg agttgccgga acttctatcg
     8281 ccataaaacc gttcacccgc gtgccagtaa ctgtgactgg cgagcgaacg cctgttggtg
     8341 gcaaaacagc acctggcggt tactcacggc aatcacgtca ttatgaaaaa cgctctggtc
     8401 gataacgtcc gggttttgct gggcgaaaat cacctgttgg ggattcacct gattaagcct
     8461 tgccaccgcc tcgctggctt cgcgagtctg tcgcgccgga taacgggaag gccgggtatc
     8521 attgccttct tctcgcccgt agacaaaaag tagcataccc ggttcaccgt aatgaccgcc
     8581 gagacgattg tggtttgccg ccccctcatc accgagcaac gctacctgcg gcaacgccga
     8641 atggacgcta aatttctctt cgtccttaaa aatcgctttt aacagcgatt cagtgacggg
     8701 cgcttccagc gaacggtgaa atttattgtt caggttggca acggtgagat gcactttgcc
     8761 atccagcgta tcggcagatg gcgcgatcgt tgccgcattg gctacccaca ttggcgaagc
     8821 ggaactgacg ctggaaagcc agtgcggtgc ctggcgtgca actttttcca gtacctgctc
     8881 atcgctacca ctgaatccca actgacgcag caccggaata aacggacgct cgtgcggcgg
     8941 aatcacggcc tgggggaatc ccgcatcggc aagggctttc attttcagta agccctgttt
     9001 tgccgccagt cgcgggttag acacctgaaa acggtgacgg gtagaggctt cattaccaaa
     9061 cgacaggccc gcgtaatgat gcgtcagccc taccagcccg tcgaaattaa cttcccaggc
     9121 gttcatcgca ccacctcatc ggaaaaatcc agcccggggt taagcgtggc gggcaatgtt
     9181 aacgagtccg actccaggct cgccataggc catgcgcagt aatctgcggc ataccaggcg
     9241 ctggggcgat ggttaccgga agcaccaatg ccgccgaatg gggcggtact ggcagcacca
     9301 gtaagcggtt tgttccagtt aacaatcccc gcccgcgcct ccagcaacag ttgatcgaat
     9361 ttttcccgct cgggggaaac cagaccgcaa aagagtccga agcgagtgtt gttcgccatc
     9421 cgaatcgcct catcaaaagt atcataacgc cagacgcgca aaaacggccc gaacacctct
     9481 tcatccggca caccgccaac gcccgtcatt tcaatgatcc ctggtgtcag caacgatgtc
     9541 cctgcttgta ataagcgcgg cgcaagcagg gttcgtccgc ccatcgcttc cagttgctgc
     9601 caggcagtaa ccacctgctg tgcggcctgt tcagaaatca gcccgccaat aaacggctgc
     9661 ggttcgtcat cccagttgcc cggcgttaat cgctggctga cggcaaccag actagcaaga
     9721 aacgcatcgc cctgcgctcc gcttttgagc aataaacggc gggcgcaggt gcagcgttgc
     9781 ccggctgtga caaacgccga ctgaatggtc agatggacag ccgcgtcgat atccgccacc
     9841 tcatcgataa ttagcggatt attaccgccc atctcaaggg cgagaatttt ctccggctga
     9901 ccggagagct ggcgatgcag ctggtagccg gtattggcgc taccggtaaa cagcaaaccg
     9961 tcgagatcct ccagcgcact cagcgcctga cccgtttcac gcccgccctg caccaggttc
    10021 agcacgcccg gcggcaagcc agcctgctgc cataaacgca ttaccgcttc gccactccac
    10081 ggtgtcagtt cgctgggttt aaagataatg gtgttacctg ccagcaatgc cggaacgata
    10141 tgtccgttcg gcaaatgacc agggaaatta tacggcccaa acaccgccag cacgccgtgc
    10201 gggcgatgtc gcaggctcgc cgcaccgtcc ggcatttcac tacgctgctc gccggtacga
    10261 acgtgatacg ccttaattga tatcgcgatt ttattgatca tcgccgtcac ttcggttgcc
    10321 gcttcccagc gcggcttacc cgtttctctg gcaataatcg cggttaattc ggctttattg
    10381 ctttccagca atccggcaaa gcgttcgaca acgacctgac gttcagccaa tgagagccgc
    10441 gcccagcgcg gaaacgccgc acgggctgcc cgacaagcct gttcgacctg agcggcatcg
    10501 gcatcattgc cttgccataa cacctcgccc gataccggat tacgcttcac acgcgatgcg
    10561 ccctggcccg ttatccagtc accgttaatc cataaagtca tgctgttttc tcctctgcgc
    10621 acaggcgcac cagacgaacg cgatcccctg cgtggcattt gagggcatcc agttgtgcgg
    10681 cggttaaaat caaacgctcg gttgccggat cggtacgcgc cagcaccacg cggaaatggt
    10741 gataattttc attggcgacc aggcaggccg gaaaatcgcc ctgcgcaggc tgcccttctg
    10801 ccacttccac cagccgactt ttacggatgg cgcgcacgcg gtcgatgtca cactcaagcg
    10861 ttggcccgcc gtcgaagatg tcgatatagt tgcggtaacg aaaaccttct ttctccagca
    10921 ccgcgcgggc aggcgcggtt tgcggatgta cctgaccgat gacgtcctgg gcttcctggg
    10981 ataaaaagtg ggtatagatc ggatgtttcg gcatcagttc tgcaataaat gccttttgcc
    11041 cggtgccgca gagaaaatcg gcgcggctaa aatccatcga aaagaagcgt ttaccgaggc
    11101 tttgccagaa cggtgaatag ccgtgttcgt caatcacccc gcgcatttca gcaaccactt
    11161 tgtcattaaa cttgtcgcga aaagccgcca taaacataaa gcgcgatttc gacagcaaat
    11221 agccgttgcc ctctttgcgc cagtccgggt cgagaaacag cgtgcacagc tcgctgctgc
    11281 cggtgtgatc gttactaaga aacagcgtcg gcaatgcgtt atagacattc agctcttttg
    11341 aggcgtgaac caacgtgccg acgcgatagt tgtaccaggg atcgttcagc ccaaccgcca
    11401 cctcaatagc gcaaatcccc gccacggtgc ctgtctcgct atcttccagc acgaacacat
    11461 agccctgctc gcttttgggc agttcacctt gccaggtttt gattgccctt tcgatacgcg
    11521 ccgaaagcgt ggcttcattg gcgggaagcg acgtcaggcc gccgcccgtt ttgctggcaa
    11581 gctgcatcag cgccgagaca tctgagcgct caacgggacg gatgaccatc atgatgaacc
    11641 tcggctaaca aagtgttcgc aagcagctgc aaagcgatcc agtccggtcg tcacctcttc
    11701 ttcgctgaca ttgagcgcag gcgcaaaacg caccacgttg ccacccgcaa tcagtaccat
    11761 cacgcctgct ttcgccgctt ccagagagat ctgtttcgct tgcccggcgt aatcggcatt
    11821 cagtacacag ccaatcaaca aacctaagcc gcgaacttca ctgaacaaac catagcggtg
    11881 attaatagta ttaagacgct caacaaacca gtcgtgacgc tgtttaacgc cattaagcat
    11941 ctctggtgtg ttgatgagct ccagcacttt gcctgctacc gccgaggcca gcggattacc
    12001 gccataggtg gtgccatgag tgccaacggt catcacgcgg gcgcactctt cggttgccaa
    12061 caacgcaccg accgggaaac cgccgcccag cgctttggcg gtggttaaca gatcgggcgt
    12121 cacgccgtag tgcatatagg catacagctc accagtgcgc ccgacgccgg tttgtacctc
    12181 atcaaaaatc agcaaggcat tgtggcggtc acacaattca cgcagacctt gtaaaaacgc
    12241 gttgctggct ggcaccacac cgccttcgcc ctagatgggc tcgacaatca ccgcacaggt
    12301 ggcgtcgtca atcagcgcgc tggcagagtt aatatcgtta tatgcagcat gacgaatatc
    12361 cgccggcagt ggcgcaaaat cctgtgaata ggctggctgc ccacccgcac tgacagtaaa
    12421 cagcgtgcga ccatgaaacg catttttgaa cgccacgatg ccactcttat ggctgccgta
    12481 gcggtcgtga gcgaatttac gcgccagttt tagcgccgct tcgttggctt ccgcaccgga
    12541 gttacaaaag aagacgcgat cggcaaacgt ggcgtcgatc aattttttcg ccagtcgcag
    12601 taccggctcg ttggtgtaac cgttgccggt atgccagaac ttactcgcct gttcgttcag
    12661 cgcttcacgc agttccggat gcgcatggcc cagcgcgttc accgcaatgc cacccgcgaa
    12721 gtcgatatac tctttcccct gctgatccca caagcgcgaa ccttcgccac gtaccggtat
    12781 aaagggtgcc ggagcgtaaa caggtatcat ccattcatca aagttttcac gcgtaattgg
    12841 ctgagacata gcgacctcta cagtaaataa ttcgttattt gtatgttaac aataagtaat
    12901 gtttgcgctg taaatgtaga ttgcagggtt cgtgccagcc agtgataaaa gtgcataaac
    12961 ggcggaggct aactggaaat caaggagtta taaccaaacc atatgcattt aaagtgcata
    13021 taaagtgaat acgtttgcga tgtgggtgaa taaaaagaat aaaaaacgca atgttatgca
    13081 gaagtaaaat ataattctag aattgtgatc attgacgaaa tttactggaa attactgcgc
    13141 cattctgacg cagcgcgcac caaaagcggg cattttttgc gccatcgttg acatcattaa
    13201 caaccatcga tcaaatcact taacaacagg cggtaagcaa cgcgaaattc tgctaccatc
    13261 cacgcactct ttatctgaat aaatggcagc gactatgaaa tttgtctctt ttaatatcaa
    13321 cggcctgcgc gccagacctc accagcttga agccatcgtc gaaaagcacc aaccggatgt
    13381 gattggcctg caggagacaa aagttcatga cgatatgttt ccgctcgaag aggtggcgaa
    13441 gctcggctac aacgtgtttt atcacgggca gaaaggccat tatggcgtgg cgctgctgac
    13501 caaagagacg ccgattgccg tgcgtcgcgg ctttcccggt gacgacgaag aggcgcagcg
    13561 gcggattatt atggcggaaa tcccctcact gctgggtaat gtcaccgtga tcaacggtta
    13621 cttcccgcag ggtgaaagcc gcgaccatcc gataaaattc ccggcaaaag cgcagtttta
    13681 tcagaatctg caaaactacc tggaaaccga actcaaacgt gataatccgg tactgattat
    13741 gggcgatatg aatatcagcc ctacagatct ggatatcggc attggcgaag aaaaccgtaa
    13801 gcgctggctg cgtaccggta aatgctcttt cctgccggaa gagcgcgaat ggatggacag
    13861 gctgatgagc tggggggtgg tcgatacctt ccgccatgct aatccgcaaa cagcagatcg
    13921 tttctcatgg tttgattacc gctcaaaagg ttttgacgat aaccgtggtc tgcgcatcga
    13981 cctgctgctc gccagccaac cgctggcaga atgttgcgta gaaaccggca tcgactatga
    14041 aattcgcagc atggaaaaac cgtccgatca cgcccccgtc tgggcgacct tccgccgcta
    14101 atttagcagc tctcctggct caaactgggt caggagaatt aaccttgaga aaaatcaaca
    14161 aactgtcagt aatgatttgt tgcctgccgt cctttgttat accgtcactg cgtttttagt
    14221 tgtctgacca cttctctatt atcaagtttg atataggaaa ctccacgatg aacgctgagc
    14281 gtaactttct gtttgcctgt cttatttttg cgctggtcat ttacgctatc cacgctttcg
    14341 gtttattcga tctgctcacc gatttacccc acttacagac actcattcgc cagagcggat
    14401 tgttcggtta tagcctctat attctgttat tcatcaatgc ccccctcttt ctgttaccag
    14461 gaagcatact ggtgatcgct ggaggaatag tttttggccc gctcttaggg acacaactct
    14521 cattaattgc cgcaacgctg gcctcctcgt gctcattcct gctggcgcgc tggctgggac
    14581 gtgatttact gctgaaatac gtgggccata gccatacctt ccaggccatt gaaaaaggca
    14641 ttgcgcgtaa cggtattgat tttcttattc tgacccgctt aatcccgttg tttccttaca
    14701 atattcaaaa ttacgcttac ggattcacca ccatcgcctt ctggccttat acccttattt
    14761 cggcactcac gaccctgccc ggtattgtta tttataccgt gatggcaagc gatctcgcca
    14821 atgaaggcat tacgctgcgc tttattttac aactctgtct ggcgggcctg gcgctgttta
    14881 ttctcatcca gctcgcaaaa ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
    14941 gccgcagccc acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
    15001 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
    15061 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
    15121 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt
    15181 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
    15241 tcgctgttta tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt
    15301 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
    15361 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
    15421 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
    15481 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
    15541 aatgcaacat acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat
    15601 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
    15661 agccaagata aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc
    15721 tcatcatcct gttgttcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
    15781 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
    15841 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
    15901 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
    15961 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
    16021 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
    16081 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
    16141 cttttgtccc tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct
    16201 cgttttttat cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag
    16261 gcagcatgtt aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc
    16321 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact
    16381 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
    16441 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
    16501 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
    16561 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt
    16621 ggatatcacc agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt
    16681 ggctgactgg cggcaaagcc cgctctttag cgatgaagaa cggctggcgc tggagtacgc
    16741 cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc
    16801 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    16861 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    16921 acgttcttaa ggagggaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    16981 ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca
//
LOCUS       DAJTUP010000083        16765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTUP010000083 DAJTUP010000000
VERSION     DAJTUP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.083"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            195..761
                     /gene="mntP"
                     /locus_tag="OO468_002877"
     CDS             195..761
                     /gene="mntP"
                     /locus_tag="OO468_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR6259921.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(758..1567)
                     /gene="rlmA"
                     /locus_tag="OO468_002878"
     CDS             complement(758..1567)
                     /gene="rlmA"
                     /locus_tag="OO468_002878"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR6259922.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1733..1942)
                     /gene="cspE"
                     /locus_tag="OO468_002879"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1733..1942)
                     /gene="cspE"
                     /locus_tag="OO468_002879"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR6259923.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(1955..2098)
                     /locus_tag="OO468_002880"
     CDS             complement(1955..2098)
                     /locus_tag="OO468_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR6259924.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2769..3056)
                     /locus_tag="OO468_002881"
     CDS             complement(2769..3056)
                     /locus_tag="OO468_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR6259925.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(3131..3274)
                     /gene="mgrB"
                     /locus_tag="OO468_002882"
     CDS             complement(3131..3274)
                     /gene="mgrB"
                     /locus_tag="OO468_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR6259926.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3433..3672
                     /locus_tag="OO468_002883"
     CDS             3433..3672
                     /locus_tag="OO468_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259927.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(3815..4606)
                     /gene="kdgR"
                     /locus_tag="OO468_002884"
     CDS             complement(3815..4606)
                     /gene="kdgR"
                     /locus_tag="OO468_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR6259928.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4783..6156
                     /locus_tag="OO468_002885"
     CDS             4783..6156
                     /locus_tag="OO468_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6259929.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(6202..7083)
                     /gene="htpX"
                     /locus_tag="OO468_002886"
     CDS             complement(6202..7083)
                     /gene="htpX"
                     /locus_tag="OO468_002886"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR6259930.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7275..9323)
                     /gene="prc"
                     /locus_tag="OO468_002887"
     CDS             complement(7275..9323)
                     /gene="prc"
                     /locus_tag="OO468_002887"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR6259931.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9343..10041)
                     /gene="proQ"
                     /locus_tag="OO468_002888"
     CDS             complement(9343..10041)
                     /gene="proQ"
                     /locus_tag="OO468_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR6259932.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10138..10635)
                     /gene="msrC"
                     /locus_tag="OO468_002889"
     CDS             complement(10138..10635)
                     /gene="msrC"
                     /locus_tag="OO468_002889"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR6259933.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     VGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10765..12048
                     /gene="yebS"
                     /locus_tag="OO468_002890"
     CDS             10765..12048
                     /gene="yebS"
                     /locus_tag="OO468_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR6259934.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12017..14637
                     /gene="yebT"
                     /locus_tag="OO468_002891"
                     /pseudo
     CDS             12017..14637
                     /gene="yebT"
                     /locus_tag="OO468_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14717..16156
                     /gene="rsmF"
                     /locus_tag="OO468_002892"
                     /pseudo
     CDS             14717..16156
                     /gene="rsmF"
                     /locus_tag="OO468_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16274..16510
                     /locus_tag="OO468_002893"
     CDS             16274..16510
                     /locus_tag="OO468_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR6259935.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16615..>16765
                     /locus_tag="OO468_002894"
     CDS             16615..>16765
                     /locus_tag="OO468_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR6259936.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFI"
BASE COUNT         4037 a         4251 c         4073 g         4404 t
ORIGIN      
        1 gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg aagactttca
       61 gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa aacccatcgc
      121 ggcttgttgt tcatgtatgc atgtttgctg ggggcgatga tgtgttttat ggataccccg
      181 gtcaggacgt tgtcatgaat atcactgcta ctgttcttct tgcgtttggt atgtcgatgg
      241 atgcatttgc tgcatcaatc ggtaaaggtg cccccctcca taaaccgaaa ttttctgaag
      301 cattgcgaac cggccttatt tttggtgccg tcgaaaccct gacgccgctg atcggctggg
      361 gaatgggcat gttagccagc cggtttgtcc ttgaatggaa tcactggatt gcgtttgtgc
      421 tgctgatatt cctcggcggg cgaatgatta ttgagggttt tcgtggcgca gatgatgaag
      481 atgaagagcc gcgccgtcgg cacggtttct ggctactggt aaccaccgcg attgccacca
      541 gcctggatgc catggctgtg ggtgttggtc ttgctttcct tcaggtcaac attatcgcga
      601 ccgcattggc cattggttgt gcaaccttga ttatgtcgac attagggatg atggttggtc
      661 gctttatcgg ctcaattatt gggaaaaaag cggaaattct cggcgggctg gtgctgatcg
      721 gcatcggcgt ccagatcctc tggacgcact tccacggtta ataagaacgc tgccagaggt
      781 gaatattaaa gtccgtctgg cagtcgaaca cttcttttgc tgccagtgtt tgccagactt
      841 ctggcttcgc acgccaggca aacggcgtca tctgcaataa tgcgacggct tcatcaccgc
      901 gaagacgcat cggataacac aactccgcac tctgctgtaa tgtaaaacct tccagttgtt
      961 ctgcatgagg tgcatgaaga tgtacttcat tgtaaatcag ccccttcagc tccatcaaat
     1021 gtcgcggtcc cggcgtggca gtaatgaccc agccgccggg cttcactact cgtgctaatt
     1081 cttctgcttt acacggcgcg taaatacgta ttatggcgtc catactggta tcggaaaacg
     1141 gcaaacggtg gctggaagcg acacaaagag tgacctgcgg atagcgtttc gccgccgctt
     1201 ttatcgctac cttcgagaca tccaggccaa acgtggtgat ttcgggcaac gcatcggcaa
     1261 atgcgtgtgt gtaataccct tcaccacagc caatatccag caccgccgtg gccttttcat
     1321 caagccgttc ccgcagttgg gcgacaattg catcacgcag cggctgataa tgtccggcat
     1381 ctaagaatgc gcggcgtgct tgcatcattt ccgcgctgtc gcccggatca cgagaccgtt
     1441 tatgctgaac gggcagcaga ttgacatacc cttctttcgc catatcaaac tgatgtcgtt
     1501 ggggacagat atagctgttt ttttcacgcg aaagaggctg atggcaaagt ggacaggaaa
     1561 acgacatgac aactccggca ggatactaaa ggccgcaagt gtaacgcgaa tcacgcggcg
     1621 ggcaaatatc cgaacgccag gagcgaagat aaagcatagt aaaaagcctc gcattcgacg
     1681 aggctttata gcgagattga agcgtattca cacttcagat cagtggattc gatcagatag
     1741 ctgttacgtt aacagctgcc ggacctttct ggccgtcctg aatttcgaac tcaacgttct
     1801 gaccttcagc cagagttttg aagccattac cctggatagc ggagaagtgt acgaacacat
     1861 ctttgctgcc atcagccgga gtaatgaagc caaaaccttt agactcgttg aaccacttaa
     1921 cctgaccttt aatctttgcc atttgaaaaa ttccttagat tgttttcttc gcccgcaggc
     1981 ataacataga taaaactgac acattactgc atgaggcacc aatataaggc tcggcagaga
     2041 agcggtattc aacgtcaacg tgtttactca ggacttcttt actgaaaatg ccacacataa
     2101 acagaactgt acctcgttta acccgaaatc ttttatcaca tacaacgtta attatggcaa
     2161 gccattttta aacatatctc gatcagacgc acaaatctcg atacgtcttc cacttttttt
     2221 gcacacttat gcaacggaat acgcgccgat atatcattgt gcttaacctt gccagttcag
     2281 gcagatactt aaactggcgt agtttctaac atagttcaat aagttcagtt gttccaggcc
     2341 agagggagaa gttattacat agtgcgtgca atatcacatt ttctgctatg caatgaataa
     2401 aaagttatat cactttttct cataaaacag tcagttaacg gctatcaatt accctaaaga
     2461 gaagtcaatc cccaaaggga ttgtaaattt aaaataagaa aaattgatga gtgagcaaaa
     2521 aaaatcaaga gagaaacgtt tcgctaataa ataataccac gcttaatcac aaatctgatg
     2581 taaacatcct aataaattaa tgaggaaaat atctttttct gatatcccgt tacgcaaata
     2641 aataatatga taattatttg aagtcagacc agagttttaa tttgaagcag ttgcaccatg
     2701 acagtgcgta taatcaggat gaattgagta acccttcaac gacggggtta ctcaagattg
     2761 aggaaggatt atcgttcggc gaccaggcga ataatatctt cgtcatcaac cgatttttga
     2821 ctctcaatgg tctttttatc tgctttctct ggttcgtttg cttcgcacaa acgacgtaac
     2881 agtgcatttt gacgtttttg ctgatccagc aacgcctcaa gcagttcaat ctgttcgtta
     2941 gtccgtgaac tggcacgatt gataaaaaac cacaagatga gcccgataag aagaaccacc
     3001 accgatacaa ccaaagacgc aatgttcatc acgcctgaat ttacaacttc gttcatttca
     3061 ccacctctga gtagaggcgc tattctacca ctgctggaga ggaagaaaat ctagtgctga
     3121 aaaaatgata tcaccacggg ataaactggt taatggcaca aattccgctg aaaaattgta
     3181 catcctgatc gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa gccaacacca
     3241 cgacaaccag aacgacccat cgaaactttt tcactccaat ctccgcttca cctaccttat
     3301 gtcattaaac tagcatgcgt tagttaaaca gcgcctaact atgtcgatat tcgtgctttt
     3361 tcggaatgag tagcttgctt cagcgcatat tgccgctacg attaagcgaa cataaaaaga
     3421 gaagagattg taatgcgatt catcattcgc acagttatgc tgatagcact ggtatggata
     3481 ggattattac tcagcggata tggcgttttg attggaagca aagagaatgc cgcaggatta
     3541 ggtttgcaat gtacctatct gactgccaga ggcaccagca cggtacaata cttgcacact
     3601 aaaagtggtt tcttggagat aacagattgc ccactgttac gtaaaagcaa tatcgtggtc
     3661 gataatggtt aagggtaata aataaccaga ttctcactca tgagtaaaat attcttatag
     3721 caagcgcgtt taatataacg caccttcaac tttatttttc tcatgctaaa taaagaacct
     3781 gtctgtttga tattccgaca ggttcttact ctcatcagaa cggatagtcg tgataaccca
     3841 tttgggcaga aattttgcgc gctgcggtat gcaacattgc gacatattcc tgtaaacgct
     3901 cttcagagaa acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc acgccaaagc
     3961 gatcaaatac cggtaccgca atgcattgca gcccttcttc ctgctcttcg ttatcttctc
     4021 catacccctg ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta ctggtgatgg
     4081 tccgctcggt actgcgttta tactccacgc cctcaagaat ttgcttcact tcatcgcgat
     4141 cgcgccatgc cagcagtacc ttaccaatcg cggtgctgta cagcggatta cgacgcccaa
     4201 tccgtgaata catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa acaatactgt
     4261 cttcgtccag tgcgccgagg tggatagttt ctttggtcag gcgggagatc tcacgcatct
     4321 ggatatctgc gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc agttcaaaca
     4381 atttcagggt cagggaatat ttctccgact ccccttcctg cgccacataa cctaaggttt
     4441 tcatggtctg taaaaagcga taaacggtgc tttttgacat catgacgcgc tgcgacagct
     4501 cggttatccc tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa acttttagca
     4561 cggaagatac agaatcaggc tgtttatcca gatctgcgtt agccatttat cacctcattg
     4621 cgagtgtttt ataaaaatca gaactgtttt ttattataat ttcgcaccag ggtggtcgca
     4681 atccatcttt tgccggttag ttacaattct gcgacatcca ccgtgaatat cagtgctaga
     4741 atcatacccc tgttgattat tcaccaaaga tataaaattc ctatgccaaa agttcaggcc
     4801 gacggcctgc cattgcccca gcgatacggt gcgatattaa ccattgtgat tggtatttcg
     4861 atggctgtcc ttgacggcgc aatcgccaac gtcgccctgc caacaattgc cacggacctt
     4921 catgccacac cagccagttc catctgggta gtgaacgcct atcaaatcgc cattgtcatc
     4981 tccctgctct cgttttcgtt tctgggcgat atgtttggct atcgacgtat ttataaatgc
     5041 ggtctggtcg tttttctgtt gtcttcactg ttctgcgccc tttctgattc gctgcaaatg
     5101 ctcacccttg cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag cgttaatacc
     5161 gcacttatcc gcctgatcta tccacaacgt tttctgggga gagggatggg cataaactcg
     5221 tttattgttg ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat cctctccatc
     5281 gcatcctgga aatggttatt tttaatcaac gtaccgttag gtattatcgc cctgcttctg
     5341 gcgatgcgtt ttctgccacc caatggttct cgcgccagta aaccccgttt cgacctgccc
     5401 agcgccgtga tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag tggtttcgct
     5461 caggggcaat cgctgacgtt aattgctgcg gaactggtgg taatggttgt tgttggtatt
     5521 ttctttattc gccgccagct ttctcttccc gtaccgctgc taccggtgga tttactgcgt
     5581 atcccgctgt tttcactttc tatttgcaca tctgtttgct ctttctgcgc acaaatgctg
     5641 gcaatggttt ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga agtcgaaaca
     5701 ggtttacttc tgacaccgtg gccgttagca acgatggtga tggctcctct ggcaggctat
     5761 ttgattgaac gcgtacatgc aggattgctg ggtgctttag ggttgttcat catggctacg
     5821 gggctttttt ccctggttct gctgcccgcg tcacctgcgg atatcaatat tatctggccg
     5881 atgatcttat gtggcgctgg atttggctta ttccagtcac ccaataacca caccattatt
     5941 acctccgctc cgcgcgaacg tagcggtggg gccagtggca tgttaggaac ggctcgtcta
     6001 ctgggtcaga gtagtggcgc ggcgctggtg gcgctgatgc taaatcagtt tggtgataat
     6061 ggtacgcacg tctcgctgat ggctgcggct attctggcag tgattgctgc ctgtgtcagt
     6121 ggtttacgta tcactcagcc acgagccagg gcataaaaaa agcgcgtcga tcaggacgcg
     6181 ctttttagta tttacttcat attacttcag gtattcaccc gtacgcagag cttcaatacg
     6241 tttatccagc ggcgggtgag tcatgaacaa ctcactgagc gatttcgact taccgttaat
     6301 gcagaaagcc atcatgctgg ttgcttcttg cggttcatag ctggttttca ggcgctgcag
     6361 cgcggcaatc attttctcgc gaccaaccag ttttgccgaa ccggcatctg catggaattc
     6421 acgatgacgc gagaaccaca tggtgataat gctcgccaga ataccaaaca ccagttccag
     6481 aaccgttgca accgcaaagt agatcagcgg gttgccgttg ctctcttcac cttcatcacg
     6541 atttccgccc ataaaacccg cggcaagctg cgccagaata cgggaaataa agataacgaa
     6601 ggtgttcacc acgccctgaa tcagcgtcat ggtgaccata tcaccattgg cgatgtggct
     6661 gatttcgtga gcaattaccg cctcggcttc atccgggctc atgttctgca gcaaaccggt
     6721 gctgacagca accagagagg catcacggcg cgcaccggtt gcaaaagcgt tgatgtccgg
     6781 cgcatggtag atagccactt gcggcatagc gatccccgcc tgacgagcct gggttgctac
     6841 agtattgacc agccaacgtt ccctttcgtt acgcggttgc tcgatcactt ccccgccaac
     6901 agatcgtaat gccatccatt tggacatcag aagcgaaacg aaggaaccac caaaaccgaa
     6961 cagcaaggcc atgatcatca gcccctgaac gctgctcgac tgtatccctg tcaggctcag
     7021 taccagcccg aaaacgacca ttacggccag gttcgttagc aggaagagcg cgattcgcat
     7081 cataattttc tttttacctc agtttaacaa aacgcaatat gcgataccca catcgtatgg
     7141 gttacgcgac tattttcaag tctggatagt gcgtaagtca ccagaaagac acaactttac
     7201 attttgtagc atctgattta cggcatcttg ccgctgttaa aaaatcaggc acaatttctt
     7261 gtgcctgatt gatattactt gacgggagcg ggttgttccg cgggtctggc tttttcaagc
     7321 ttcgccagat cgagtgcgat attcaccgtc tcatccagat aaggatccgg ctcctggtaa
     7381 tctttcgata gatcatccag cttcttcaac tccggtttac cttctcgttt aaagcgttcg
     7441 ttcaaacgcg ccagacgcgt cgcatcatct tcattattct ctttctcacg cacagcgtag
     7501 ttcagagaaa cgatattgcg cttgtccttc atagcgttga agcgcgcgat atccttcatg
     7561 atgttctgga actcaggatc tttcgcgata cgcgcattat gttccttcag cagctccggt
     7621 tcaaaggcag ttaaatctcc tgatttcaca taagtcgcgg catcgatgct atcccacggc
     7681 agcgcgttat cttcgaattt ctcacccgtt tccgtttctt cattacccgt cggcatgatg
     7741 atgtctggtg ttacgccttt acgttgcgta ctgccgccgt taacgcgata gaatttctgg
     7801 atcgtgtact gcacagaacc cagcgctggc cattcaggac gtaacatctg atcgtaaata
     7861 cggttcaatg aacggtattg ctgaacggtg cctttaccaa acgtcggttc acccacaacc
     7921 agcgcacgac cgtaatcctg cattgccgcg gcaaagattt ctgaagccga agcactgaaa
     7981 cggtcaacca gtaccaccag cgggccttta tagaacacct gcccgtcggt atcgctgtct
     8041 tcacgaacct tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg aataaacaga
     8101 cctgagagcg ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag gtcgatgatc
     8161 acactgctga cattctgttt ttccagtttc tgcagttgaa ctttgacatc gtctgtcaaa
     8221 cccacataga agcccggaat atccagcacg ccgactttct ctttaccgac ggtcttcacc
     8281 gacattttaa ccgcgcggtc ttcgagacga atacgttcac gggtcaacgt tacagtacgg
     8341 gtcttggtcc ctttaccagc aggtaaaatt tccagacgaa ctttactgcc cttcggccct
     8401 ttaattaagg caaccacatc atcaagacgc cagccaatca cgtcaaccat cggcttgcct
     8461 gtttgaccaa caccgacaat tttgtcacca acgctgatag ctttactctt cgctgccgga
     8521 ccacctgcca ccatcgaatt gataacggtg tagtcatcat ccatttgcag cactgcgcca
     8581 ataccttcca gtgacaaact catttcagtg ttgaactgtt cggtattacg cggggaaaga
     8641 tagttggtat gcggatcgat ttcacgcgca aacgccgtca ttgccagcga gaaaacatct
     8701 tcgctgttgg tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt cagggtttca
     8761 cgaatttctt tatccgtttt tcctgtcagc ttcaggctta actcgtcgaa tttgacttta
     8821 ctgtcccact gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt gctgcggtca
     8881 aggttataag tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga caaagcgtac
     8941 tggtaacgtt caaaacggcg cttttgcgcc agattgtaga gatcgtagaa aacgtcgagt
     9001 ttgcctgaac gcagttcatc gcctaactcg gttttctttt tcgcgaactg ttcaacatcg
     9061 cttgccagca gcacattgtg gctgtaatcg agcagattca ggtagcggtc aaagattttg
     9121 gccgaaaatg cctgatcgag gtcgaactgg cgataatgag aacgggtgaa gcgcgacgtt
     9181 acgcgctcac ttaccgtcgc atgctgcgtc tcttccttta ataccggaat ttgatcagca
     9241 cgcgtgatat cttctacagc gaaggtctgg cctgctattg caagcaggcc agctaacgcg
     9301 gtaagcctaa aaaacatgtt catgcctggc ccggcctccg tttcagaaca ccaggtgttc
     9361 tgcgcgcaca atcaaagaca tacccgaatt cagctggacg cggacgccgt ctttggtgat
     9421 ttctaatacg gtggcatcca tcgcgttttg acccgctttc accttcaggg cttgtccgac
     9481 agtcagagct gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg cttttactgt
     9541 ttttggcgct ttctctaccg gcttttgcgc acgaggttta cgttcagcgc cttctttgcg
     9601 gcgtggcgtg gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt tctcaccagc
     9661 agctgcggca gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct gaacacgcgc
     9721 tttcgcttct tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca gctcaccgca
     9781 tgggttgccg tcaagatcga cacgcgttgc gccgggttta acaccgtaaa gataacgcca
     9841 gctcgaagtg tagagacgta aagcggatcg taattgcgtt ttgctcaggt tcatttcccc
     9901 ggcaacacga tcgaccaaat cctgaaaaat accaattttc agcggacgcg cttcaccttc
     9961 cgcactgaaa cagtggggaa aacgttcggc cagaaacgcg attacttctt tactgctatt
    10021 caacttaggt tgattttcca tgaaatttcc tgattacaac ggacgtagcc aacaagcgca
    10081 ggcatgaaca ggcgtcatta taatgacgct accagtaaat gctacgttat ccgttgatta
    10141 tcctgcgacg ctcgcaaaga attttttgta atccgtcgtt gcaagcactt tttcaagctg
    10201 tgccacaagc tgacgtaagc cttgctcgtc ctcgtctgta aagcgagcga agacagtact
    10261 atcgatgtcg agaacaccaa taatctgatt tttcaccacc agcggcagaa caatttcaga
    10321 attactcgcc gcatcacagg caatatgccc gtcaaacgcg tgaacatcct cgatacgctg
    10381 cacttgattg cgggcaaccg ctgtgccgca caccccgcgc ccgacgggta tccggacaca
    10441 ggcaattttg ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca aataaaaacc
    10501 tacccagtta acgtcagtga gacgctcgta taacaacgca ctggtgttcg caagcgttgc
    10561 cagaaaactg gtttctcccg ccatcagcgc gttaaagtcg cgatttaaat ccgcgtaaaa
    10621 ttctgttttg ttcattactt aatcacttgg ttgtcttacc tggaactgcc agcctattaa
    10681 aataagcatt aaatgcgtta atgctcaaga tcattcccat catgggttaa gattaatgtt
    10741 aattcttatt acatttggca cgtcatggct cttaacacac cacaaattac gccgacaaaa
    10801 aagataacag tgagggcaat cggcgaggaa ctgccgcgtg gtgattacca acgttgcccg
    10861 caatgtgaca tgctgtttag cctgcccgag ataaattctc atcaaagtgc ctattgtccg
    10921 cgctgtcagg caaaaattcg cgacgggcgc gactggtcgc taacgcgcct ggcggcaatg
    10981 gccttcacca tgctgttgct tatgccgttt gcctggggcg aaccgctgtt gcatatctgg
    11041 ctgttaggca ttcgtattga cgccaacgtc atgcaaggca tctggcaaat gaccaaacag
    11101 ggcgatgcga taacgggatc aatggtcttt ttctgcgtta ttggtgcccc cctcattctg
    11161 gtgtcctcca tagcatattt atggtttggt aaccgactgg gaatgaattt acgtccggta
    11221 ctgctgatgc ttgagcgact taaagagtgg gtaatgctgg atatctacct ggtcggcatt
    11281 ggcgttgctt ctataaaggt acaggattat gcccatatcc aggcgggtgt cggcttgttc
    11341 tcttttgtgg cgttggtgat tttaacgacg gtgacgttgt cacatcttaa tgttgaggag
    11401 ctgtgggagc gatattatcc gcagcgcccc gctacgcgta gggacgagaa acttcgtgtc
    11461 tgtcttgggt gccattttac cggctatcct gatcagcgtg gtctctgccc gcgttgccat
    11521 atcccgctac gcctgcgtcg tcgtcatagt ttgcaaaaat gctgggcggc gctgttagcg
    11581 tcaatcgttt tgttgttacc agccaaccta ttgcctattt ctatcattta tctgaatggt
    11641 ggccggcagg aagatacgat tctttccgga attatgtcgc tggcaagtag caacattgcg
    11701 gttgcgggaa tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt gatcgtcatg
    11761 ttcactttac tgttgagcat tcattttaaa tgccagcaag gtttacgcac acgcattctg
    11821 ttactgcgga tggtgacctg gattggacgc tggtcgatac tcgacctgtt tgtcatatct
    11881 ttaaccatgt cgctgattaa tcgcgatcag atcctcgctt ttactatggg accggctgcg
    11941 ttttattttg gcgctgccgt aattttgact attcttgctg tggaatggct ggacagccgc
    12001 ttactttggg atgcacatga gtcaggaaac gcccgcttcg acgactgaag cgcagattaa
    12061 aaataaacgc cgtatctcac ctttctggct gctgcctttc atcgcgctaa tgattgccgg
    12121 ttggctgatt tgggacagtt atcaggaccg gggtaatacc gtcaccatcg actttatgtc
    12181 ggcggatggt attgttccgg gccgtacgcc tgttcgttat cagggcgttg aagtcggaac
    12241 agtgcaggat atcagcctca gcgacgatct tcgtaagatt gaagtcaagg tcagcatcaa
    12301 gtccgatatg aaagatgcgc tgcgcgaaga gactcagttc tggctgatga cgccaaaagc
    12361 atcgttggca ggtgtctccg ggctggacgc cctcgtcggt gggaactata tcggcatgat
    12421 gccgggtaaa ggtaaagagc aggatcactt tgtcgcgctc gatacccaac cgaaatatcg
    12481 gctggacaat ggcgatctga tgatccacct gcaagccccc gctctcggtt cgctgaacag
    12541 cggttcattg gtctatttcc gcaagatccc ggtgggaaaa gtctacgact atgccatcaa
    12601 tcccaacaag caaggcgtgg tgattgatgt cctgatcgag cggcgtttta ccgatctggt
    12661 gaaaaaaggt agccgtttct gaaacgtttc cggcgttgat gccaacgtca gtatcagtgg
    12721 cgcgaaggtg aaactggaaa gtctggcggc actggttaac ggtgcgattg ccttcgattc
    12781 acctgaagag tcgaaacctg ccgaggcgga agataccttt ggtctgtatg aagatctggc
    12841 ccacagccag cgtggagtaa taataaaact ggaactgccg agtggagcag gattaaccgc
    12901 cgactcgacg ccgttaatgt atcaggggct ggaagtcgga cagctgacta aactggattt
    12961 aaatcctggt ggtaaagtca ctggggaaat gaccgttgat ccctgcttcg tgaaaatacc
    13021 cgcatcgaat tacgcaaccc gaaattatcc cttagcgatg ctaatctcag cgccctgctg
    13081 accggaaaaa cctttgagtt ggtgcccggc gatggcgagc cacgcaaaga gttcgttgtt
    13141 gtgccgggcg aaaaagccct gctgcaagag ccggacgttc tgacactgac cctgacagca
    13201 ccggaaagtt acggtattga tgcgggtcag ccgctcattc ttcacggcgt gcaggtaggc
    13261 caggttatcg atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc catcgagcct
    13321 cagcatcgag aactggtaaa aggcgatagc aaatttgtcg tcaacagccg tgtcgacgtg
    13381 aaggtggggc tggatggcgt tgagtttctc ggtgccagcg cctcagaatg gattaacggc
    13441 gggatacgta ttctgccggg cgataaaggc gagatgaaag ccagctatcc actgtatgcc
    13501 aatctggaaa aagcgctgga gaacagcctt agcgatttac ccaccacaac catgagtttg
    13561 agtgcagaga cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg taaatttgaa
    13621 gttggtgaag ttattaccgt gcgtccgcga gctaacgcgt tcgatatcga tctgcacatt
    13681 aaaccggaat atcgcaacct tttgaccagc aacagcgtgt tctgggcaga aggcggggcg
    13741 aaagttcagc tgaatggtag tggcctgacc gtacaggcat ccccgctctc cagagcatta
    13801 aagggggcca ttagcttcga taacctcagt ggtgccagcg ccagtcagcg taaaggcgac
    13861 aagcgaattc tgtatgcttc cgaaacagcg gcccgtgcgg ttggcaggca gattacgctt
    13921 cacgctttcg atgccggaaa actggcggtc gggatgccaa ttcgctatct cggtattgat
    13981 atcgggcaaa tccagacgct ggatctgatt accgcacgca atgaagtaca ggcaaaggcg
    14041 gtgctctatc cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt ctcagtggtc
    14101 acaccgcaaa tctcggcagc tggcgttgag catctagata ctatcctcca gccgtatatc
    14161 aacgtcgaac caggccgtgg caatcctcgt cgcgactttg aattagaaga ggccaccatt
    14221 actgattcgc gttacctaga tggtttaagc attattgttg aagcgccgga agccggttcg
    14281 ttaggcatcg gtacgcctgt gctgttccgt ggtctggaag taggtacggt taccggaatg
    14341 acgctgggga cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa acgctatcaa
    14401 cacctggtgc gtaacaattc cgtcttctgg ttggcatcgg gttacagtct ggactttggt
    14461 ctgacgggcg gcgtagtgaa aaccggcacc tttaaccaat ttatccgtgg cggcatcgcc
    14521 ttcgccacgc ctccgggtac gccacttgca ccgaaagccc aagaaggcaa acacttcctg
    14581 ttgcaggaaa gtgaaccgaa agagtggcgt gaatggggaa ctgcgcttcc caaataatgc
    14641 ccactgctcc ggcgtgcctg cgccggagcg cttatgctaa actgcgcgcc tgtttttttg
    14701 ccagtggtac atgctcgtgg cccaacacac cgtttatttc ccggacgcgt ttctgacaca
    14761 aatgcgcgaa gctatgcctt cgacgctctc atttgatgat tttcttgccg cctgtcagcg
    14821 cccgttgcgc cgcagcattc gcgttaatac gctgaaaatc tccgttgctg atttcctgca
    14881 attaaccgct ccttatggct ggacgcttac gccaattccg tggtgtgaag aaggtttctg
    14941 gattgaacgc gacaatgaag atgcattgcc attgggtagt accaccgagc atttaagtgg
    15001 cctgttttat attcaggaag ccagttcaat gttgcccgtc gccgccttgt ttgctgacgg
    15061 taatgcacca cagcgggtga tggatgtcgc tgctgcgcca ggctcaaaaa cgacgcaaat
    15121 tgccgcgcgg atgaataacg aaggggcaat ccttgccaat gagttttccg ccagttgggt
    15181 aaaagtgtta catgccaata tcagccgctg tggcatcagt aatgttgcgc tcacacattt
    15241 tggtggccgc gtgtttggtg cggcagtgcc agaaatgttt gatgccattt tgctggacgc
    15301 tccctgctcc ggcgaaggcg tggtgcgtaa agatcccgat gcgctaaaaa actggtcacc
    15361 cgaaagcaat caggaaatcg cagctaccca acgggaactg attgacagcg cctttcatgc
    15421 attacgtcct ggcggtacgc tggtttactc gacctatacc ttaaaccggg aagaaaacga
    15481 agccgtttgc cggtggctga aagagactta ccccgacgaa gtagagtttt taccacttgg
    15541 cgatctcttc cctggtgcaa acaaggcgct gaccgaagaa ggttttttgc atgttttccc
    15601 acaaatttac gactgcgaag gcttcttcgt tgctcgtctg cgtaaaactc aggcgatccc
    15661 cgccttaccc gccccaaaat acaaagtggg caatttcccg ttcagcccgg tgaaagatcg
    15721 cgaagccgga caaattcgtc aggcggctgc aagtgttggc ttaaactggg atggaaacct
    15781 gcgactctgg caacgcgaca aagaactgtg gttgttcccg gtaggcattg aagccctgat
    15841 cggtaaagtc cgattttctc gcttagggat taaacttgcc gaaacgcata acaaaggtta
    15901 tcgctggcag catgaagcgg ttattgccca tgcctccccc gacaatgtaa acgcttttga
    15961 actgacaccg caggaagcgt aggagtggta tcgcgggcgc gatgtttacc cgcaagccgc
    16021 gccagtggcg gatgacgtgt tggttacttt ccagcatcag ccgattggtt tagccaaacg
    16081 gattggttcg cgactgaaaa acagctaccc gcgtgaactg gtgcgggacg ggaaactttt
    16141 taccagtaac gcatgacagc gcacaaaaaa agcgcacttt ttgactggca cattcgccgg
    16201 cctcaactag gctgaaaaat tgtgcgatcg gactggtcgt actacaatca gcagctaaat
    16261 ggagagcacg aagatgaaaa ccagtgtgcg cataggcgct tttgaaatcg acgacggcga
    16321 attacacggt gaatcgccgg gtgatcgaac gttaaccatt ccttgtaaat ctgaccccga
    16381 tttatgtatg caactggatg cctgggatgc tgaaaccagt atccctgccc tgctaaatgg
    16441 cgaacactct gtcctttacc gtacccgtta cgatcaacaa tctgatgcct ggattatgcg
    16501 tcttgcctga tccaaaaaga acccgtcggc atggcgggtt atttgtcctg gttattcccc
    16561 cgttgtaaaa tctctcctaa acttaacggt acggcaccac acttcgggga tgaaatgttc
    16621 gcgctggtac tttttgtttg ctacctggat ggcggttgtg aagatattgt tgtggatgtc
    16681 tacaacacgg aacagcagtg tctttattct atgagcgatc aacggatccg ccatggcagt
    16741 tgttttccga ttgaggattt tatag
//
LOCUS       DAJTUP010000084        16516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTUP010000084 DAJTUP010000000
VERSION     DAJTUP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.084"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(83..2524)
                     /gene="rnr"
                     /locus_tag="OO468_002895"
     CDS             complement(83..2524)
                     /gene="rnr"
                     /locus_tag="OO468_002895"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR6259937.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2563..2988)
                     /gene="nsrR"
                     /locus_tag="OO468_002896"
     CDS             complement(2563..2988)
                     /gene="nsrR"
                     /locus_tag="OO468_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR6259938.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3193..4491)
                     /gene="purA"
                     /locus_tag="OO468_002897"
     CDS             complement(3193..4491)
                     /gene="purA"
                     /locus_tag="OO468_002897"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR6259939.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4595..4792)
                     /locus_tag="OO468_002898"
     CDS             complement(4595..4792)
                     /locus_tag="OO468_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR6259940.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4874..5878)
                     /gene="hflC"
                     /locus_tag="OO468_002899"
     CDS             complement(4874..5878)
                     /gene="hflC"
                     /locus_tag="OO468_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR6259941.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5881..7140)
                     /gene="hflK"
                     /locus_tag="OO468_002900"
     CDS             complement(5881..7140)
                     /gene="hflK"
                     /locus_tag="OO468_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR6259942.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7226..8506)
                     /gene="hflX"
                     /locus_tag="OO468_002901"
     CDS             complement(7226..8506)
                     /gene="hflX"
                     /locus_tag="OO468_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR6259943.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8582..8890)
                     /gene="hfq"
                     /locus_tag="OO468_002902"
     CDS             complement(8582..8890)
                     /gene="hfq"
                     /locus_tag="OO468_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR6259944.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8976..9926)
                     /gene="miaA"
                     /locus_tag="OO468_002903"
     CDS             complement(8976..9926)
                     /gene="miaA"
                     /locus_tag="OO468_002903"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR6259945.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9919..11766)
                     /gene="mutL"
                     /locus_tag="OO468_002904"
     CDS             complement(9919..11766)
                     /gene="mutL"
                     /locus_tag="OO468_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR6259946.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11776..13113)
                     /gene="amiB"
                     /locus_tag="OO468_002905"
     CDS             complement(11776..13113)
                     /gene="amiB"
                     /locus_tag="OO468_002905"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR6259947.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13132..13593)
                     /gene="tsaE"
                     /locus_tag="OO468_002906"
     CDS             complement(13132..13593)
                     /gene="tsaE"
                     /locus_tag="OO468_002906"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR6259948.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13565..15112)
                     /gene="nnr"
                     /locus_tag="OO468_002907"
     CDS             complement(13565..15112)
                     /gene="nnr"
                     /locus_tag="OO468_002907"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR6259949.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15111..16250
                     /gene="queG"
                     /locus_tag="OO468_002908"
     CDS             15111..16250
                     /gene="queG"
                     /locus_tag="OO468_002908"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR6259950.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16233..16286)
                     /locus_tag="OO468_002909"
     CDS             complement(16233..16286)
                     /locus_tag="OO468_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259951.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3751 a         4716 c         4145 g         3904 t
ORIGIN      
        1 ataaggcgtt cacgccgcat ccggcatgaa caaagcgcat tttgtcagca atctaaccct
       61 cttcttttaa agagggtatt gatcactctg ccactttttt cttcgccgca cgcttcgctt
      121 tggtcgctgc ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt
      181 tcttcgccgc tttcggcttc gtttttttct caccgcggaa ggcgctgtct ggctcaaagt
      241 ttaccttttt accgacctga caacgcttgc cgcctttttt gcctgcatcg ccttttttcg
      301 ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg ttcactggag atcagactaa
      361 agtcgatttt gcgctcgtcc atattaaccg cttcgacgcg aacttccacg cgatcgccca
      421 ggcgataagt ctggccgctg gattccccca tcaggcgttg ccctacctgg tcaaagcgat
      481 agtagtcatt gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac
      541 ggacgaagaa gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct
      601 ggtcgagcat gaagtcacac ttcagccagt cagcgacatc gcgcgttgct tcgtcggcac
      661 gacgttccgc catcgaacag tgctgaccca gttgcaacat ctcttccatc gaataatggt
      721 agccgccggt ttcagtggtg ttgccctgat gcccctgctc tttcgccagc agatatttaa
      781 tggcgcggtg cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg
      841 actgcaatgc cagaccgaag tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg
      901 agcgtagcag catggtttgc agcatttctg catcaggacg gtcggcaacc gactccagca
      961 gttccgcgta gtcacgcggt tccggcttgt taccacccgg cagctccagc cccagctccg
     1021 ccagcactga acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca
     1081 gtgccggttc tttcgctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc
     1141 actcttcaat taatttgtgc gcgtcgttac gctgggtctg ttcgatacgt tcaatacggc
     1201 gttcagcgtt gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt
     1261 cacgggcttt atccagcact ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg
     1321 gggcgtactg ctcgcgcaga tcctgatcgc cctgcagaat atgccagact ttggtgtagg
     1381 tcagacgcgc gtgagagctc atcaccgctt cgtagaattt gtagcccgtc aggcggcctt
     1441 tcgacgaaac cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgaac
     1501 acaggccgtt agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg
     1561 acgtgccacg gttacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga
     1621 cgtcggcaat cgcgacccat aaacgccagc cgccgccgcg ttttttctcg cagtaaactg
     1681 catcgtcaaa gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat
     1741 cgacacggcc cgcttttgct tcttccggca cttcttcttt cagccccgca acctgttgct
     1801 caacagcctg cggccagata tacggaattt catgggtacg cagagcgata tcaaccgcca
     1861 tgccggtgcc catattgtcg cccagcactt cgacgatttt acccaccgct ttggtgcggc
     1921 gagtcggacg ctgagtcagt tcgactacga ccacaaagcc catccgcgcg cccatgatct
     1981 gatcgggcgg gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga
     2041 cgcccgcttc ggtaaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga
     2101 caatacgcgc ttcacgacga cctttacggt cagcacccag cggctgcgcc agcacctgat
     2161 cgccatgaat gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt
     2221 caacccgcag aaagccgtag ccatcacggt gaccaataac ggtacctttc accaggtcga
     2281 ggcgttccgg cagcgcatag cactgacgac gagtgaagac cagttgacca tcgcgctcca
     2341 tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttcta
     2401 ccgccagctc atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt
     2461 cccgactagg gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg
     2521 acatctcggt tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa
     2581 tttataaagc ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt
     2641 aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg
     2701 gcaaaactca ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc
     2761 aatacgtatc gcactcgccg gtttacccag gcgaatgccg ccattttttc cacgaacagc
     2821 agtcacgtag ccggcacgac taagttgatt gattattttg accatatgat tacgggagac
     2881 gccgtagacg tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat
     2941 gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa
     3001 agggaaatcg ggaaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct
     3061 aatttatttc agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta
     3121 atggcttaac cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca
     3181 ggcgtaccag aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg
     3241 accggtagag atgatatcga tcggcacacc ggtcagctct tcaatacgct tgatgtagtt
     3301 cagcgccgcc tgcggcaggc cgctacgatc tttcacgccg aaagtggatt cagaccagcc
     3361 cggcatggtt tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt
     3421 agtcacttcg cgaccatccg gcatacggta agccacgcag agtttaacct ctttcaggcc
     3481 atccagaacg tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg
     3541 acgaacggca acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcaccgaa
     3601 ttcgttaccc tgcttgcaga ggaactcgcc agtttcatca aacagttcgg ccgggaaagg
     3661 acctgcacct acacgagttg agtaagcttt gaggataccc agaacgtaat caacataacg
     3721 cgggcccagg ccggaaccgg tcgccacgcc accagcagtg gtgttggaag aagttacgta
     3781 cggataagta ccgtggtcga tatccagcag cgtaccctgc gcaccttcga acatgacgaa
     3841 atcgccacgc tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag
     3901 gatgtcggca acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt
     3961 gtagtagtta accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa
     4021 ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata
     4081 agcaggcccg ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg
     4141 cgcgttatcc agagcaacgt gataatcaag gatcagcgga catgcttcag acagcagcag
     4201 acgctcacga acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcggc
     4261 cggagacagc acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga
     4321 tggaataaga tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg
     4381 accgccctgg tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc
     4441 tttaccttcg tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa
     4501 tcaccgtttg cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc
     4561 actcaaaaat gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt
     4621 agtagaccac aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg
     4681 gcaaattggt catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta
     4741 aaccttccag taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc
     4801 cttatagaaa aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac
     4861 cgcagtcgtt atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc
     4921 tatccgggct catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca
     4981 ggctacggat gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg
     5041 ctgcttcggc atcgccttca ccacgcataa tgcggccctg acgctcagct tctgccagcg
     5101 ttctggtcac ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg
     5161 aacggtgacg acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag
     5221 acacttcggt cggcaggttg atctgcttga tacgcacatc gacaacttca atacccagcg
     5281 ccgccatact gttcgggttg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct
     5341 ctgccgcttc ggcaatggcg ttatctgctg ccggggtagt aacttcatct tctgtacccg
     5401 cagaaccgga gttcagcgcg tcacgtactt cgagggtcag acgaccacgg gaatcggtga
     5461 cgatatcttt cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt
     5521 tcaacagcac ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag taacggctga
     5581 aatcgctgat gcgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca
     5641 caaagcggtc ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt
     5701 caatgaacgg tatcttgaaa tgcagaccag gctcataaac caaaggtttg ttgtcatcgt
     5761 cacgcagtac cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga
     5821 cagacatgta aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg
     5881 ttattccccc tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat
     5941 atcgccctga ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg
     6001 cggcagacgc agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc
     6061 tttcagcatc tggtctaacg gcagaaccat caggttgcca cctttatcgt taaccagcac
     6121 tttgcgggtg ttacccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat
     6181 ttccggcgcg gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc
     6241 ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc
     6301 gttcgcacgc ggctgaactt cgttggaata cgcttctgct tcacgaatgt attgctgttc
     6361 gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt acttcttccg gcggacgagc
     6421 agcctggaag ttgacgtcca gcagcgtgat acccatgtca tacggacgaa tcgtctcttc
     6481 cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat
     6541 ggtgtattta ccgataactc cacgcagggc gctgtcggta gcctgacgca ggctgtcatc
     6601 cgggctggtc acgctataca gatatttttc cggattggtg acgcggtact gcacgttcat
     6661 ctcaacgcgc actacgttct cgtccgacgt cagcatcaca ccagaagcgg ccagttcacg
     6721 cacggcttcc acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc
     6781 cggctcaacc agatggctga atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt
     6841 aatggtatag aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac
     6901 gacacgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt
     6961 gcctttaccg ccgcccagac caccgagctt tttgctcagt ttgcggaaga tatcatctaa
     7021 atcaggtggc ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgccagg
     7081 tttgctgctt ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat
     7141 gtttgtgctc catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg
     7201 actccacgct tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt
     7261 tacagaggcg acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct
     7321 cctccatcca ctctttttct attgcctgaa gctgataaaa acgactcctc agacgccctt
     7381 cctgcggtgg cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag
     7441 cctgaaaaag ctgtggtatc cccgctccgg tctgtgcgga aagccagaca cggatcggtt
     7501 tgttctcttc gtcccgatca atacgcggtt cgaaatcttc cagcatatcg atcttgttca
     7561 tcaccagcag ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt
     7621 cgatgttttc ttgtacacgc acatccgccg catcaatgac gtgcagcagt aatgtggctt
     7681 gccgcgtctc ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa
     7741 taaaccctac ggtatctgca agtacggttt caccgacatc tgcaacgtca atacgccgca
     7801 acgtcgggtc gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc
     7861 gattgaaaag ggtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt
     7921 cggctttgat acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg
     7981 actgtatctg cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac
     8041 ccggaccacg caaacctatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca
     8101 ggcgcgtagc cagatggcgc agctgcgcca gctcaacctg caacttaccc tcatgggtac
     8161 gcgcacgttg ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca cgacactcgc
     8221 acaaacgctc caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg
     8281 aagcacccat cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat
     8341 actttgggtg cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg
     8401 aagagaccag agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga
     8461 tgtgtaccag taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct
     8521 aaatagatca gcggggaacg caggatcgct ggctccccgt gtaaaaaaac agcccgaaac
     8581 cttattcggt ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat
     8641 ggtagttact gctggtaccg ccaccggcgt tgttactgtg atgagaaacc gggcgagatg
     8701 ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga
     8761 tcacgaactg atcaaaagac tcgatttgcc cttgcagctt aataccattc accaaataaa
     8821 tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc
     8881 ccttagccat tctctctttt ccttatatgc ttatttgtac tttgaacctt tcgattctga
     8941 aaaaattgcg cacgatacgt ctcaattgta cacattcagc ctgcgatagc accaacaacc
     9001 tgtaatactt cgtcacgcgc ctgttctggt ttttcactgt caagccagtg aaccccttcc
     9061 caaccacgca gccaggttat ctgccgcttc gccaactgtc tcgtggcgca aacacctcga
     9121 taaaccattt catcgtatga gatttcgcct tcaaggtaag accacatctg gcgataaccc
     9181 acgcaacgaa tggaaggcaa gtccgtatgc aaatctcctc gggcaaaaag cgcccggact
     9241 tctgcttcaa aacctgaagc caacatctga tgaaaacgct gctcaatgcg ttgatggagc
     9301 agttcacggc tcgccggggc gatggcgaac tgatgcacct gatacggtag agcgtctcct
     9361 gacgtttgcg tcagttccgt taaagtttta cccgaaatga aaaaaacttc cagtgcccgg
     9421 gaaagccttt gtggatcatt tggatgaatc cttgctgccg caaccggatc tacctcctga
     9481 agttgacgat gcaatgactc ccaaccttgc tctgccgcct gttgctcaat tctggcccgc
     9541 acttccgggt ctgccgacgg tagcggcgac aacccttcca gcaatgcctt gaaatacaac
     9601 atcgtaccgc ccactaacag tgggatccgc cccgccgcgg tgatatcggc catttccgcc
     9661 agcgcatcgc ggcgaaaatc agcagccgag taagcctgcg acggatcgcg aatatccagc
     9721 aatcggtgcg gcgcggcgag taactcttca gcgttcggct tcgccgtccc gatatccatc
     9781 cctttgtaaa taaggacaga atcaacgctt atcaactcta ctggtaaaat tttacgcagc
     9841 tcaatggcta acgccgtttt accggaggcc gtcggcccca tcaaaaaaat cgccttaggc
     9901 aggctcgcct tactgatatc actcatcttt cagggctttt atcgccggat gtaaatcaac
     9961 agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc gttccacgtc
    10021 cgccagcagg gttatggcct gtgccattga ccactgcgca tgttcgctca tcagatttcg
    10081 tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca ggtagcctat
    10141 cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca cggccctgat
    10201 ggtcacatgc tgtgcatctg actggaaatc aatacccaat tccgctaggg cagactgcgc
    10261 tttttctaat gccgattttt cttcgccaga aacttttagc cgcaacggaa tcagcagcgg
    10321 ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc tgacgcagcc aacgttctgc
    10381 cactggcaag gataaaagtg aaatgttgcc atcgcgctcc agcaacgcac agtcggaatg
    10441 gacgatagtc agtacccgac caaaactctg actgttcgcc gcaagtgcag gttcctgcgg
    10501 ttccggcgct tttggttttt gcatcggcgc gggcgtttgc aaaagctggc gatacacttc
    10561 accttgctgt ttctggtagc ctggctgcgc attcggccag ggggcagccg gacgactgcc
    10621 tgaggcgggc gcaggagagt agcgcggagc taccggctca cgagctgccg gttctgcaaa
    10681 gtgattgcgc cccgccgcca cgcggttttc cgggatggca cgcggtgcgg gttgtggttc
    10741 atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca cgccctgata
    10801 gataaaatca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg cggggtgtac
    10861 gttgacgtcc acctgatgcg ggtcgatctc cagatacaac acaaatgccg gttgctgatc
    10921 ggcccccagt ttgtcttcgc aggcctggcg gatcgcatga ttgatcaggc gatcgcgcat
    10981 catgcgacca ttcacgtagc aatactgaat ttccgccagt gcgggcgtag tgtgatttgg
    11041 atcggccacc cagccgcgca gggtgagatc gccgtgttgc cattcaatcg ccagcgcttg
    11101 ttcgagaaaa gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg
    11161 cactgcgcgg tactgacgca caattttacc gttatgcgac aggttgatcg tgacgtcgaa
    11221 acgcgccagc gcaatgcggc ggatgatttc atcaatatgg ctaaattcgg ttttctcggt
    11281 gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct ccagcgtcgt
    11341 tcccaccgga tgcgctgccg gtttaaccgt cacgtccatg tcgcgtcctt cggcataggc
    11401 ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact
    11461 gatactcgcc agcgcctcac cgcgaaagcc caggctgata atggcttcga gatcgtccag
    11521 agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat cttttttgat
    11581 accgcagccg ttatcacgaa tgcggataag tttcgcccca ccgcgttcga tatcaatatc
    11641 gatacgcgtc gcacctgcat cgaggctgtt ttccactagt tctttgacta ccgacgcagg
    11701 tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta agacctgaat
    11761 tggcatcaat cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg tactggcagt
    11821 ttgtgccgtt gcaccctgcg gcgcagattg catcggatgc gcaaggaagt aattgcgcag
    11881 gcctttgtaa atggcttctg ccagctgttg ttggtaatcg tcgctcgcca gcaaacgttc
    11941 ttcgctgttg ttgctgataa aaccggtttc gaccagtact gatgggatat ccggcgagcg
    12001 cagaacgcca aggctggcgt gttctggtcg acgtttatgt atttcgccaa tgcgttgcaa
    12061 ctgactgatc atactggtcg ctacatcata ccctacccgc tgggaatgac cgaactgtaa
    12121 atccagcacc gcctggctta aataggggtc agactgactg ttcgccagca catcacccgc
    12181 cccacccagc agctccgaca gtttctcgtg ctgctccagc cagctggcca tttcactgtt
    12241 ggcgcgacgg ttagagagca cccatacgga agcgccagtc gcactacggt tcggtgcggc
    12301 atcagcgtga atcgacacga ggaaattggc gttttgctta tgtgccacat cactgcgccc
    12361 catcaccgag ataaagtaat ccccgtcacg ggttaaaacg cctttaaaca tcggatcgtc
    12421 attgagcaaa gtacgcaatt tacgcgcgat ggcgatggtg acatttttct cccgcgtacc
    12481 accggggccg atagcgccag ggtcctgacc gccgtgtccg gcatcaatag cgatgataat
    12541 tttatcgcca gtgttagccg tcgcgcgcgc tgccggacgc gttaccgtat tactgctgat
    12601 aacacccgta gtgcggttac tttccgtttt aaacggattg cgcgccggtt cgctgacgcg
    12661 cggtgcgaca accgcaggcg tttcaacgcg tttcgcaacc acgggcggcg gaggaggcgg
    12721 tggcggcaca tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat tctgccgctt
    12781 caccgcttcg gttttaccgt tttcggtaag atcgaccacc agccgtagcg tttgtgcatc
    12841 tttaggcgtt ccagagcgaa tcgccttcac cagattattg ccgctgaaca acaacggcag
    12901 tccctgaatc atgcccgttt gtttgatatc gagcgccacg gtgcgtttgc tttgatggct
    12961 aaacgcataa tcaggatcgc caataaaact caacgttatc cgcgcctgtt gattaccgtt
    13021 agaaacctga atatcagaga gcgtcgcggc acccaccggc gtgcacagca gcagcagcgt
    13081 cgctaccaac caatttctga tgcgatacat catcccgcca cctttcaaag gttaaccggc
    13141 taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg cctcacggcc
    13201 ttgtgcctga taatcaatgt gtatttcgac atccgggtca ggaagaacac ctgtaccttg
    13261 ttgtggccac tccaccaggc agatggcatc gttggcaaaa taatcgcgga tccccataaa
    13321 ctccagctcc tcgggatcgg caaggcggta caaatcaaag tgatagacca ttaagttgtc
    13381 gagcgtatag ggttcgacca gcgtataagt ggggcttttg acattaccct gatgacccag
    13441 agcctgtaaa aagccccggc taaaggtggt tttacctgcg cctaaatcgc catacagata
    13501 gattacggtt gcgccatcgc aggctttcgc tacccgctcg cccaggtcta atgttgcctg
    13561 ctcatcaggg agcggaatta ctcgattcat catggttttt atcagtcact tccgggttaa
    13621 caatacgctg tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt ccaaaacgcg
    13681 ccgccagtac gtcagctgcc gcaccgtgcg cgacacagcc tgcacaggct gcatcatacg
    13741 gcgacagttt ttgcccaagc aatgcgccaa taataccaga gagcacatcg cccatgccgc
    13801 cgctcgccat gcctgcattt ccggcatcaa taatgcctaa agcgtcagga tgggcggcga
    13861 ccacggttcc ggcacctttc agcaccgcta cgccgccata acgttgtacc agacgtttgg
    13921 cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca gcctaacaac cgtgcggcct
    13981 cgctaggatg cggcgtgatc acgcgattgt gacgcttatc gggattgatt gccagcaggt
    14041 tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt tgcagggctt
    14101 ttttccccca ctcttgctgg cccagaccgg gaccaatcac caccacatcg gcccattcca
    14161 ggctttcggt aagagagtcc atagtcagtt catgcaccat caattctggt cgtgcagtca
    14221 gcagcggcgc aatgttctca ctgcgggtca gtactcggac taaaccagca ccagcacgta
    14281 gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca ccgataatca
    14341 ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg cggtttcagc cagtgagaaa
    14401 gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc tgccagccag ctatccagcc
    14461 ccagtgagtc aaaatgcagt tgtcctgtaa catcccgcgc ttttccagtg agcaagccta
    14521 gtttcagcgc aataaaagtg atggtgtgat cggcgttgat cactgcgcct ggcgtagcgc
    14581 cggtttcagc cagcaggccg gaagggatat caaccgccac aatcggcgca ggatgggaat
    14641 tagcgtggtc gattaactgg ctaatggatt cgcggggcgc ttgctgcaag ccggtaccga
    14701 gcagcgcatc aacaatcaga tctaccgatt cgggccagac aatattcgaa gcatggatct
    14761 cgccacccgc gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc aacggtttgt
    14821 cgctctcctg ggccaacaac gtgacctcaa tgccgaccgc tttggccagt cgcgcgacca
    14881 cgtagccatc gccgccgtta ttaccatgac cgcacagcac cagccagtgg cgggcgtcag
    14941 gatacgccga acgacacacc tggaatgcgg cctcgccagc gcgaagcatc agctcataga
    15001 gtgtgagccc cagcacatct gccgcctcgc gttctccgcg gcggatatcg tcggcgtacc
    15061 agacggtgtg tggtatactt acggggtttt tcttcattgt atggtccgtc atgtcagagc
    15121 ccctcgatct caatcagtta gcgcaaaaaa ttaaacagtg ggggctggaa ctgggctttc
    15181 agcaggtagg tattaccgat accgatctca gcgagtccga gcccaaactg caagcatggc
    15241 tggacaaaca ataccacggc gaaatggact ggatggcacg tcacggtatg ctgcgcgctc
    15301 gcccccatga gttattaccc ggtacgctgc gtgtaatcag cgtgcggatg aattaccttc
    15361 ctgctaacgc cgcatttgcc agcacgctga aaaaccccaa actcggctat gttagccgtt
    15421 atgcgctggg ccgtgactat cacaaacttc tgcgcaaccg actcaaaaag ctgggcgaga
    15481 tgattcagca acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct gcgcctattc
    15541 tcgagcgccc gttagctgaa aaagctgggc tcggctggac aggtaagcac tcacttatcc
    15601 tcaatcgcga ggccggttcg ttcttctttt taggcgaatt gctggtcgat attccgctgc
    15661 ccgtggatca accagtcgag gaaggatgcg gcaaatgcgt ggcctgtatg acgatttgcc
    15721 cgaccggtgc catcgtcgag ccatataccg tcgatgctcg ccgctgtatc tcttatctca
    15781 ccatcgaact tgaaggggcg ctcccggaag agttgcgacc gttaatggga aaccgtattt
    15841 acggttgcga tgactgccag cttatctgcc cgtggaatcg ctattcgcaa ctcactacag
    15901 aagacgattt cagcccgcgt aagccgctac acgcaccgga actcattgag ttatttgcct
    15961 ggagcgaaga gaagttttta aaagtcacgg aaggatcggc gattcgccgt ataggtcacc
    16021 tgcgctggct gcgtaatatc gccgtggcat taggcaatgc cccttgggat gaaacgattt
    16081 tgacagcgct ggaaagtcgt aaaggtgagc acccacttct cgatgagcac atagcgtggg
    16141 cgattgcgca gcaaatagag agacgaaatg cgtgcatagt cgaagtgcaa ttgccgaaaa
    16201 aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga ttcatccaca
    16261 gcttgtgaat aaaaataaaa acgcattgca attcaagacg aataaaatcg tcaagtgatc
    16321 gactcgacaa aacggaatct tgatttttaa cttatttatc aataggttaa aaaaatacca
    16381 ttcaccgtgc gaagtttctt tgaaattaag attacagcct gaggctgtgg ataactctgt
    16441 ttacaagagt ttgtcagaga caacaaaaac cagcaataac gcatcgcgtc gctgtagata
    16501 ttttattgag agaaga
//
LOCUS       DAJTUP010000085        16430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTUP010000085 DAJTUP010000000
VERSION     DAJTUP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.085"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            138..821
                     /locus_tag="OO468_002910"
     CDS             138..821
                     /locus_tag="OO468_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR6259952.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            865..5527
                     /locus_tag="OO468_002911"
                     /pseudo
     CDS             865..5527
                     /locus_tag="OO468_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            5895..7550
                     /gene="lldP"
                     /locus_tag="OO468_002912"
     CDS             5895..7550
                     /gene="lldP"
                     /locus_tag="OO468_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR6259953.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7550..8326
                     /gene="lldR"
                     /locus_tag="OO468_002913"
     CDS             7550..8326
                     /gene="lldR"
                     /locus_tag="OO468_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR6259954.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8323..9513
                     /gene="lldD"
                     /locus_tag="OO468_002914"
     CDS             8323..9513
                     /gene="lldD"
                     /locus_tag="OO468_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR6259955.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9709..10182
                     /gene="trmL"
                     /locus_tag="OO468_002915"
     CDS             9709..10182
                     /gene="trmL"
                     /locus_tag="OO468_002915"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR6259956.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10235..11056)
                     /gene="cysE"
                     /locus_tag="OO468_002916"
     CDS             complement(10235..11056)
                     /gene="cysE"
                     /locus_tag="OO468_002916"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR6259957.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11136..12155)
                     /gene="gpsA"
                     /locus_tag="OO468_002917"
     CDS             complement(11136..12155)
                     /gene="gpsA"
                     /locus_tag="OO468_002917"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR6259958.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12155..12622)
                     /gene="secB"
                     /locus_tag="OO468_002918"
     CDS             complement(12155..12622)
                     /gene="secB"
                     /locus_tag="OO468_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR6259959.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12685..12936)
                     /gene="grxC"
                     /locus_tag="OO468_002919"
     CDS             complement(12685..12936)
                     /gene="grxC"
                     /locus_tag="OO468_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR6259960.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13078..13509)
                     /locus_tag="OO468_002920"
     CDS             complement(13078..13509)
                     /locus_tag="OO468_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR6259961.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13754..15298
                     /gene="gpmM"
                     /locus_tag="OO468_002921"
                     /gene_synonym="pgmI"
     CDS             13754..15298
                     /gene="gpmM"
                     /locus_tag="OO468_002921"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR6259962.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15308..>16430
                     /gene="envC"
                     /locus_tag="OO468_002922"
     CDS             15308..>16430
                     /gene="envC"
                     /locus_tag="OO468_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR6259963.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLY"
BASE COUNT         4120 a         4261 c         4293 g         3756 t
ORIGIN      
        1 ttttatgatg aatgcttaaa acaatattta atcgacacac atcactgtca ttaatgtaaa
       61 gcaacatagc tcaccaataa tacaatggac aactctaccg gcattgagcg ctggtcggag
      121 gaaataagga tttttacatg aacttgaaga agattttttt cagtgctgtc actgtttcag
      181 ttctctgcgc attaacggga tgtgactaca ttgaagaagg caagccagaa agtagtttac
      241 tgaaacaaca agaagaacat aataataaaa ttgtgctact tgaaaaacaa caagcacagc
      301 taaagagtca actcgaaact attcaaaaac aacaaactgg cataataaac agtacaaaga
      361 ctttaactca cgtaattaaa tccgttaaag accaacaaaa tacttttatt tttaccgagt
      421 ttaacccagc aaaaacgaag tatttcatcc tgaacaacgg ttcggtggct ttagcgggtc
      481 gagtgttatc cattgacgcc acagagaatg gtagtgttat tcatatctca ctggtcaact
      541 tattaagtac acctatctca aacattggtt ttaatgcgac atggggtggt gaaaaacctg
      601 tcgatgccaa agagtttgcc cggtggcaac aattactttt caacacatca atgaaatcca
      661 cattgaaatt attaccaggt caatggcaag acattaattt gaccctgaag ggtgtatcgc
      721 ccaataacct gggatatctg aagttagcca tcaacatgga aaatattcag ttcgacaatc
      781 ttccctctgc tgaaaatcgg cagaaaagaa gcaaaaaata aatgcagtta gtacgcttat
      841 aacaaggaat agcgagcatc attaatgaac aaaatattta aagttatctg gaaccctgcg
      901 acagggaatt atactgttac cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc
      961 agtaagctgt taatttctgc gctggttgcg ggtggaatgt tgtcgtcgtt tggggcattg
     1021 gcgaatgccg ggaatgacaa cggtcagggt gttgattacg gtagtggatc agctggcgac
     1081 ggctgggttg ctataggcaa aggggcgaaa gcaaatactt ttatgaacac cagtggttcc
     1141 agtactgctg tgggttatga cgctatagct gaaggccaat atagctctgc catcgggtca
     1201 aaaacccatg cgattggtgg tgcatcaatg gcctttgggg ttagtgcaat atcagaaggc
     1261 gatagaagta tagcactggg tgcctcttcg tattcattgg gccaatactc aatggccctc
     1321 ggccgttatt caaaagcatt gggtaaattg tctattgcta tgggggactc ttccaaagcg
     1381 gaaggagcaa acgccattgc cctggggcgt agcagtgtag ctagcggtac tgacagcctc
     1441 gcatttggca gacaatcact tgccagcgca gcgaacgcta ttgcgatagg tgctgagacc
     1501 gaagccgctg aaaatgcaac tgctattggc aataatgcga aggcaaaagg gactaatagc
     1561 atggcaatgg ggttcggaag ccttgccgat aaagtcaata ctatcgcatt aggaaatggc
     1621 agccaggctc tggcagataa tgcaatcgcc ataggccagg gcaacaaagc tgatggcgtg
     1681 gatgccatcg ctctgggtaa tggtagccag tcgagaggct taaacaccat tgccttaggc
     1741 acagccagta atgcaactgg tgataagagt cttgcgcttg gtagtaatag cagtgccaac
     1801 ggtattaact ctgtcgcgct gggcgcagat tccattgcgg atttagacaa taccgtctct
     1861 gtcggcaata gttcattaaa acgcaagatc gttaatgtga aaaatggcgc gatcaagtct
     1921 gacagttacg atgccattaa tggttcacag ctttatgcca ttagcgactc ggtagcaaaa
     1981 aaggcttgga ggaggggctg cagtagatgt tgatgacggt actgttacag caccaaccta
     2041 caatttaaaa aatggtagca aaaataacgt aggggctgcg ctcgctgtac ttgatgaaaa
     2101 caccctgcaa tgggaccaaa ccaaaggcaa atacagcgct gctcatggta ctagtagccc
     2161 aactgccagc gtaatcaccg atgttgcgga tggcacgatt tcagcctcca gtaaggatgc
     2221 ggttaacggt tcccaactga aagctaccaa tgacgatgtc gaagccaaca ccaccaatat
     2281 caccaacctg acggattccg ttggtgacct tcaggctgat gccctgctct ggaacgaaac
     2341 taaaaaggca ttcagtgcag ctcacggcca ggataccacc agcaaaatca ccaacgttaa
     2401 agatgccgac ctgacggctg acagcactga tgctgttaac ggctctcagc tgaaaaccac
     2461 caacgatgct gtggcgacga ataccaccaa tatcgccaat aacacttcca atattgccac
     2521 taacaccacc aacatctcta acctgactga gacggtgact aatcttggtg aggatgcgct
     2581 gaaatgggat aaggacaatg gtgtattcac ggcagctcat ggcaccgaga ccaccagcaa
     2641 aataaccaac gttaaagatg gcgacctgac gactggcagc accgatgccg ttaacggctc
     2701 tcagctgaaa accaccaacg atgccgtggc gacgaatacc accaatatcg ccactaacac
     2761 caccaacatc tctaatctga ctgagacggt gactaatctt ggtgaggatg cgctgaaatg
     2821 ggataaggac aatggtgtct tcactgcagc tcatggcaac aataccgcca gcaaaatcac
     2881 caatatcctg gacggcacag tcactgcaac cagttccgat gccattaacg gtagccagct
     2941 ttatgactta agcagcaata tcgccaccta cttcggcggc aatgcttctg tgaatactga
     3001 cggtgtgttt accggtccaa cctacaaaat cggtgaaaca aattattata acgtcggcga
     3061 tgcactggct gcgattaact cctcatttag cacgtctctc ggcgatgctc tgctttggga
     3121 tgccaccgca ggtaaattca gtgccaaaca cggtactaat ggtgacgcaa gcgtgatcac
     3181 tgatgtcgca gatggtgaaa tttcagactc cagttctgac gcagtaaacg gctcacaact
     3241 ccacggcgtg agcagttatg ttgttgatgc gctggggggt ggtgccgaag tcaatgcaga
     3301 cggcaccatc actgcgccga cgtacaccat tgctaatgct gattacgata atgtcggtga
     3361 tgccctgaat gctatcgata ccactcctga cgacgctctg ctctgggatg cggacgccgg
     3421 tgaaaatggt gcatttagcg ccgctcacgg aaaagataaa actgccagtg taatcactaa
     3481 cgtcgctaac ggtgcaatct ctgctgccag cagcgacgcg attaacggct cacaactcta
     3541 taccaccaat aagtacatcg ctgatgcgct gggtggtgac gcagaagtca acgctgacgg
     3601 caccatcacc gcaccgactt acaccattgc gaacgccgag tacaacaacg tcggtgacgc
     3661 cctggatgcg cttgatgata acgccctgct gtgggataag actgccaatg gcggtgctgg
     3721 agcctacaat gccagccatg acggtaaagc cagcatcatc actaatgtcg ctaatggcag
     3781 tattagtgag gacagtaccg atgcagtgaa cggttctcag ttgaatgcga cgaatatgat
     3841 gattgagcag aacacccaaa ttatcaatca gctcgctggt aacaccgacg caacctatat
     3901 cgaagaaaat ggcgctggta tcaactacgt tcgtactaac gacaacgatt tagcctttaa
     3961 cgatgcaagc gcctctggtg tcggcgctac agctgtaggt tataacgctg tcgcgtctgg
     4021 tgccagcagc gtagccattg gtcagaacag cagcagcacc gttgataccg gtattgcgct
     4081 gggtagcagc tccgtttcca gccgtgtgat tgccaaaggt tctcgtgaca ctagcgtaac
     4141 ggaaaatggc gtggtgattg gttatgacac cactgacggc gaactgctag gtgcattgtc
     4201 aattggtgat gacggtaaat accgccaaat catcaacgta gctgatggtt cagaagccca
     4261 tgacgccgtt acggttcgcc agttgcagaa cgctattgga gcggtcgcca ctacgccaac
     4321 caagtacttt cacgccaact caacggcaga agactcactg gccgttggtg aagactcgct
     4381 ggcaatgggt gcgaaaactg tcgttaatgg taatgcaggt attggtatcg gtttgaacac
     4441 tctggttctg gctgatgcga tcaacggcat tgctatcggc agtaacgcac gcgcaaacca
     4501 tgcaaacagt atcgcaatgg gtaacggttc tcagaccacc cgtggtgcac agactggcta
     4561 caccgcctac aacatggacg caccgcagaa ctctgtaggt gagttttctg tcggtagtga
     4621 agacggtcaa cgtcagatca caaacgtcgc agctggttca gcggataccg atgcggttaa
     4681 cgtgggtcag ttgaaagtca ctgatgagcg cgtagcgcaa aatacccaga gcattactaa
     4741 cctgaacaat caggtcacta atctggatac tcgcgttact aatatcgaaa acggtattgg
     4801 cgacattgtc accaccggta gcaccaagta cttcaagacc aacaccgatg gcgtagatgc
     4861 caacgcccag ggtaaagata gcgttgctat tggttctggt tccattgctg ccgctgacaa
     4921 cagcgtcgca ctgggtaccg gttccgttgc agaggaagaa aatacaatct ctgtaggttc
     4981 ttctactaac caacgccgta tcaccaacgt agctgccagt gttaatgcca ccgatgcggt
     5041 taacgtgtcg cagctgaaat cttctgaagc aggcggagtg cgctacgaca ccaaagctga
     5101 tggttctatc gactatagca atatcaccct cggtggtggc aacggcagta cgactcgtat
     5161 cagcaacgtc tccgctggcg tcaacaacaa cgacgcggtg aactacgcgc agttgaagca
     5221 aagcgcgcag gaaacgaagc aatacaccga tcagcggatg gttgagatgg ataacaaact
     5281 gtctaaaact gaaagcaagt tgagcggtgg tatcgcttct gcaatggcaa tgaccggtct
     5341 gccgcaggct tatacaccgg gtgccagcat ggcttctatt ggtggcggta cttacaacgg
     5401 tgaatcggca gttgctttag gtgtatcgat ggtgagcgcc aatggtcgtt gggtctacaa
     5461 attacaaggt agtaccaata gccagggtga atactccgcc gcactcggtg ccggtattca
     5521 gtggtaatga tccattaagt tagtgtgact aagggcaaac gccaaattgc ctgatgcgtt
     5581 acgcgcatca ggcaagataa ggcgctccgg gtcaacaatc taagggcaat tctctgatga
     5641 ggattgccct tttctttacc agacatctcc ccccacaaga attggcccta ccaattcttc
     5701 gcttatctga cctctggttc acaattcccc aattaaaact cacatcaata ttgccaatac
     5761 ataacattta gttaaccatt cattgtcatt atccctacac aacacaattg gcagtgccac
     5821 ttttacacaa cgtgtgacaa ggagatgagc aacagactca ttacacgatg tgcgtggact
     5881 ctaggagacc tgcaatgaat ctctggcaac aaaactacga tcccgccggg aatatctggc
     5941 tttccagtct gatagcatcg cttcccatcc tgtttttctt ctttgcgctg attaagctca
     6001 aactgaaagg atacgtcgcc gcctcatgga cggtagcaat cgcccttgcc gtggctttgc
     6061 tgttctataa aatgccggtc gctaacgcgc tggcctcggt ggtttatggc ttcttctatg
     6121 gtttgtggcc catcgcgtgg atcattattg cagcagtgtt cgtctataag atctcggtga
     6181 aaaccgggca gtttgacatc attcgctcgt ctattctttc gataacccct gaccagcgtc
     6241 tgcaaatgct gatcgtcggt ttctgtttcg gcgctttcct tgaaggagcc gcaggctttg
     6301 gcgcaccggt agcaattacc gccgcattgc tggtcggcct gggttttaaa ccgctgtacg
     6361 ccgccggact gtgcctgatt gttaacaccg cgccagtggc atttggcgcg atgggcattc
     6421 caatcctggt tgccggacaa gtaacaggca tcgacagctt tgagattggc cagatggtgg
     6481 ggcgtcagct accgtttatg accattatcg tgctgttctg gatcatggcg attatggacg
     6541 gctggcgcgg tatcaaagag acgtggcctg cggtcgtggt tgcaggcggc tcgtttgcca
     6601 tcgctcagta ccttagctct aacttcattg ggccggagct gccggacatt atctcttcgc
     6661 tggtatcact gctctgcctg acgctgttcc tcaaacgctg gcaaccagtt cgcgtattcc
     6721 gcttcggtga cttgggggcg tcacaggttg atatgacgct ggcccacacc ggttacaccg
     6781 cgggtcaggt gttacgtgcc tggacaccgt tcctgttcct gaccgctacc gtaacactgt
     6841 ggagcatccc gccgtttaaa gccctgttcg catcgggtgg tgcgctgtat gagtgggtga
     6901 tcaacattcc ggtgccgtac ctcaataaac tggttgcccg tatgccacca gtggtcagcg
     6961 aggctacagc ctatgccgcc gtgtttaagt ttgactggtt ctctgccacc ggcaccgcca
     7021 ttctgtttgc tgcactgctc tcgattgtct ggctgaagat gaaaccgtct gacgctatca
     7081 gcaccttcgg cagcacgctg aaagaactgg ctctacccat ctactccatc ggcatggtgc
     7141 tggcgttcgc ctttatctcg aactattccg gactgtcatc aacactggca ctggcactgg
     7201 cgcacaccgg tcatgcattc accttcttct cgccgttcct cggctggctg ggggtattcc
     7261 tgaccgggtc ggatacctca tctaacgccc tgttcgccgc gctgcaagcc accgcagcac
     7321 aacaaattgg cgtctctgat ctgttgctgg ttgccgccaa taccaccggt ggcgtcaccg
     7381 gtaagatgat ctccccgcaa tctatcgcta tcgcctgtgc ggcggtaggc ctggtgggca
     7441 aagagtctga tttgttccgc tttactgtca aacacagcct gatcttcacc tgtatggtgg
     7501 gcgtgatcac cacgcttcag gcttatgtct taacgtggat gattccttaa tgattgtttt
     7561 acccagacgc ctgtcagacg aggttgccga tcgtgtgcgg gcgctgattg atgaaaaaaa
     7621 cctggaagcg ggcatgaagt tgcccgctga gcgccaactg gcgatgcaac tcggcgtatc
     7681 acgtaattca ctacgcgagg cgctggcaaa actggtgagc gaaggcgtgc tgctcagtcg
     7741 acgcggcggc gggacgttta ttcgctggcg tcatgacaca tggtcggagc aaaacatcgt
     7801 ccagccgcta aaaacgctga tggccgatga tccggattac agtttcgata ttctggaagc
     7861 ccgctacgcc attgaagcca gcaccgcatg gcatgcggca atgcgcgcca cacctggcga
     7921 caaagaaaag attcagcttt gctttgaagc aacgctaagt gaagacccgg atatcgcctc
     7981 acaagcggac gttcgttttc atctggcgat tgccgaagcc tcacataaca tcgtgctgct
     8041 gcaaaccatg cgcggtttct tcgatgtcct gcaatcctca gtgaagcata gccgtcagcg
     8101 gatgtatctg gtgccaccgg ttttttcaca actgaccgaa caacatcagg ctgtcattga
     8161 cgccattttt gctggtgatg ccgacggggc gcgtaaagca atgatggcgc accttagttt
     8221 tgttcacacc accatgaaac gattcgatga agatcaggct cgccacgcac ggattacccg
     8281 cctgcccggt gagcataatg agcattcgag ggagaaaaac gcatgattat ttccgcagcc
     8341 agcgattatc gcgccgcagc gcaacgcatt ctgccgccgt tcctgttcca ctatatggat
     8401 ggtggtgcat attctgaata cacgctgcgc cgcaacgtgg aagatttgtc agaagtggca
     8461 ctgcgccagc gtattctgaa aaacatgtcc gacttaagcc tggaaacgac gctgtttaat
     8521 gagaaattgt cgatgccggt agcactggca ccggtgggtt tgtgtggcat gtatgcgcgt
     8581 cgtggcgaag ttcaggcagc caaagcggcg gacgcgcatg gcattccgtt tactctctcg
     8641 acggtttccg tttgcccgat tgaagaagtc gcgccagcca tcaagcgccc aatgtggttc
     8701 cagctttatg tactgcgcga tcgcggcttt atgcgtaacg cgctggagcg agcaaaagca
     8761 gcgggttgtt cgacgctggt tttcaccgtg gatatgccga caccgggcgc acgctaccgt
     8821 gatgcgcatt caggtatgag cggcccaaat gcggcaatgc gccgctactt gcaggcggtg
     8881 acgcatccgc aatgggcgtg ggatgtgggc ctgaacggtc gtccacatga tttaggtaat
     8941 atctcagctt atctcggcaa accgaccgga ctggaagatt acatcggctg gctggggaat
     9001 aacttcgatc cgtccatctc atggaaagac cttgagtgga tccgcgattt ctgggatggc
     9061 ccgatggtga tcaaagggat cctcgatccg gaagatgcgc gcgatgcagt acgttttggt
     9121 gctgatggaa ttgtggtttc taaccacggt ggccgccagc tggacggtgt actctcttcc
     9181 gctcgtgcgt tgcccgctat tgcagatgcg gtgaaaggtg atatcgccat tctggcggat
     9241 agcggaattc gtaacgggct tgatgtcgtg cgtatgattg cgctcggtgc cgacaccgta
     9301 ctgctgggtc gtgctttcct gtatgcgctg gcaacagcgg gccaggcggg tgtagctaac
     9361 ctgctaaatc tgatcgaaaa agagatgaaa gtggcgatga cgctgactgg cgcgaaatcg
     9421 atcagtgaaa ttacgcaaga ttcgctggtg caggggctgg gtaaagagtt gcctacggca
     9481 ctggctccga tggcgaaagg gaatgcggca tagtcgtttg cgccccctca ccctaaccct
     9541 ctccctcagg gagaggggac cgttcggcgc tgtatgtact ccctcactct gaaacgacac
     9601 cgcactcttt ttttctccct cgcccctccg gggagggccg gggtgagggg aaaaggccgc
     9661 acggttcacc aattttctgc tatcctgccc ccgcactaag ggggcagtat gctaaacatc
     9721 gtactttacg aaccagaaat tccgccaaat actggcaaca tcatccgtct ttgcgctaat
     9781 accggctttc gtctacatat catcgaaccg atgggatttg cctgggacga caagcgcctg
     9841 cgccgcgcgg ggctggacta tcacgagttt accgccgtta cgcgtcatca tgactatcgc
     9901 gcgttcctcg aagcagaaaa tccccagcgc ctgtttgccc tcaccacgaa aggtacgcct
     9961 gctcacagcg ccgtaagcta tcaggatggc gattatctga tgttcggccc ggaaacacgc
    10021 ggcctgccag cgagcattct tgatgccctg cccgctgaac aaaaaattcg cattccgatg
    10081 gtgccggaca gccgcagcat gaatctgtcc aatgcggtgt cggtagtggt gtatgaagcc
    10141 tggcggcagc tgggatatcc gggagcgttg ttgagagatt agttactgta tgccggatag
    10201 atgattacat cgcatccggc acgatcacag gacattagat cccatcccca tactcaaatg
    10261 tatggttaat accgttgaaa tgctggtcca tatccattga tggcttatcg ctgtctggtt
    10321 taccgacaat acgagccgga acgccagcgg cggttgtatg cggcggcacc ggttgcagca
    10381 ccacggaacc tgcgccaatc ttcgcgccgc gaccaacttc aatattgccg aggattttcg
    10441 cgcccgcgcc aatcatcaca ccttcacgaa ttttcgggtg acggtcacca ccagatttac
    10501 ccgtaccgcc aagcgtcaca gattgcagaa tcgatacgtc gttttcaatt accgccgttt
    10561 cacccacaac gatgcccgtg gcgtggtcga gcataatgcc acggccaatt tttgctgccg
    10621 ggtgaatatc gacctggaac gtcacagaaa cctggttttg cagaaagatt gccaatgcgc
    10681 gacgcccctg attccacaac cagtgaccga tgcgataggc ctgcaaggca tgaaaaccct
    10741 tcaggtataa caacggggtt gagtatttat cgactgccgg gtcgcgggta cgcaccgcct
    10801 gaatatcaca ggccgcagag gcgatcattt ccgggtcagc ggcgtaggct tcttcaacca
    10861 cttcacggat agcaatagca ggcataattg gcgatgacag cttgttcgcc agcatgtagc
    10921 tcagtgcact gccaaggttt tcgtgcttga gtagcgtcgc gtggtaaaaa ctggccagca
    10981 ttggctcaca gtccgccagc gttctggctt cggctttaat attgttccag acaatttcca
    11041 gttcttcaca cgacattgct tactccacac gatgagataa tgaccggccc gttctgcgcg
    11101 ggccgggtca tagcggtaac aaaggttccc tggggttagt gactgctgcg ctcgtccttg
    11161 cgtgcacgac ctaataatgt caatgctgcc tcgcgcgcgt tttttccgca atataatact
    11221 tgataaattt cctcggttat tggcatttca acgccgaagc gatgcgccag ttcgcggact
    11281 tctttcgtat tgcggtagcc ttccaccacc tgaccaatct tctcctgcgc gctttgtaca
    11341 tccatgccct gaccgagcat cataccaaaa cggcggttac gcgactggtt gtcggtacag
    11401 gtaagcacca gatcgcccag ccccgccatg cccataaagg tggcaggatc ggcacccagc
    11461 gccgcaccaa gacgcgacat ttcagccagc ccacgggtga tcagcgccgt acgcgcattc
    11521 gcaccaaaac cgataccgtc agacatcccc gcgccaatgg caatgacgtt tttcaccgcg
    11581 ccgccaagct gcacgccaat gaaatccggg ttgctgtaaa cgcggaaact tttgccgcag
    11641 tgcagcagct gctggagatc atcggcaaag gtctgatcgg tcgaggccag cgaaatagct
    11701 gtcggtaaac ctgccgccag ttctttcgca aacgttgggc cagagataac cgccagcgga
    11761 atttgatcgc ctaaggcctc acgcgccacg tcctgtaaca gacgtccggt ttccgcttcc
    11821 agccctttgg tcgcccacac cagacgcgca tcaggacgca tcagcggttt aatctggcgc
    11881 agcacttcac caaagacatg gctgggtacg acgacgagaa tattacgact ggctgccagc
    11941 gcagtggcga gatcgctttc aagatggagc gtatcgggaa aaggcacatc ggggagaaac
    12001 gcggcgttac agcggtcgcg ttcaagcgtt gcgatatgtt cagggtcatg gccccagagg
    12061 acaacctcgt ggccatttct tgccagggta atggcaagag cggtgccgta cgagccggca
    12121 ccgatcacag tcattgaagc attacgttgg ttcatcaggc atcctgatgt tcttcagtac
    12181 cttcgccagc ctgctgctgc aaatagttca tgaacagcgc atcgaagtta accggcgcaa
    12241 ggttcagttg cgggaatgta ccgcgggata ccatgctggt gatgcactca cgagcatacg
    12301 ggaacagaat gttcgggcag tatgcgccca ggcaatgtgc catctgggtg ccttcgatac
    12361 ccgcgatgga gaaaataccg ccctgctgaa cttcacacag gaacgcggtt tcttcgccca
    12421 aggaggccgt tacggtaaca cgcagtacca cttcgtatac gtcatctgcc agttgggaag
    12481 atgccgtatc cagatcaagt ttaacttctg gttgccaatc tttctggaaa acgtgcggcg
    12541 cgttcggcgc ttcgaaagag atatccttgg tataaatacg ttggatctgg aaagtcattt
    12601 cagtgttgtt ttgttctgac atgtgtagaa aacccttaag tgttgtcctt aaatactgcg
    12661 caatgtaata ccaacgttca cacgttattt cagcagggga tccagtccac cacgtgcatc
    12721 caatgcatac aagtcatcac agccgccaat gtgctgtgcg tcaataaaaa tctggggaac
    12781 cgtggtgcaa ccgctgcgtt tgatcatctc ttcacgcttg gcggcattgc catcgatcgg
    12841 caactcctgg aaactcacgc ccttgctgct cagcagtgct tttgcacgat ggcaatacgg
    12901 gcaggtttct ttggtataga tttcaacatt ggccataatt tagctcctgt ttttttaccc
    12961 tgaaatttca tgttgcaggg cggctgcaaa atggagtgtc cccagaagct tataacaata
    13021 agtcgctggg ggcctaattg cagctaacgg cctgcatcat gaaagacgac aggtaaatta
    13081 tttgccgcgc accagaggca agttttcgcc agcccagcca gcaacgccct ctttcagcac
    13141 gaatacttgc gcaaaaccag ctttcgtcag cgcgtttgca ggctcctggc actgcatgcc
    13201 agaaccgtct accacgataa ccgatttgtc tttgtgcttc tcaagctcac caacattgtt
    13261 ggctttgatt tcgctcggca acagattgat agaacctgcg atatggcctt tacggaagtc
    13321 atcacgctga cgtaaatcca caaccacagc gtcttctttg ttgatcagac gcgtagcttc
    13381 accacgagta atcaccttca ctttcgaggt caggctctta aacgtagtca caagaaccgc
    13441 caccagtaac gcgatccagg cgatactcag tatgggatga cggccaacaa attgcataat
    13501 ttcttgcatg gggggtaaca actcccgacg tagtgattaa aaaaaccagg aaaggagtat
    13561 acctgcgcgg tgcggcaaat acagccagcg cgttaactgg aatgcaattt tgcggggcac
    13621 gacgaaaaaa aagcgcaaat ctggcatcgg cttgccgcga gcggtcgtat attttgatct
    13681 tcagaggcta ttttatcgat tcagctgtag taaaattacg caaattttga ctcttgagta
    13741 tgaggttgtc gcaatgtcgg tttctaaaaa acctatggta ctggtgattc tggatggcta
    13801 tggctatcgt gaagaacagc aggataacgc catttttagt gctaaaaccc cggtaatgga
    13861 tgcactgtgg gccaatcgtc cgcataccct gatcgacgct tccggtctgg aagtcggtct
    13921 gcctgaccgt cagatgggta actccgaagt aggccacgtt aacctgggtg ccggccgcat
    13981 cgtgtatcag gacctgactc gtctggacgt tgaaatcaaa gatcgcgctt tctttgctaa
    14041 tccagtgctg gctggcgcgg tacataaagc gaaaaacgca ggtaaagcag tacacattat
    14101 gggtctgctc tccgcaggcg gcgtacacag ccacgaagat cacatcatgg cgatggtaga
    14161 actggcagct gaacgcggcg cagaaaaaat ctacctgcac gcattccttg acggtcgcga
    14221 caccccgccg cgcagtgctg aatcctcgct gaaaaaattt gaagagaaat ttgctgcgct
    14281 gggtaaaggc cgcgtagcgt ccatcattgg tcgttactac gcgatggacc gcgataaccg
    14341 ttgggatcgc gttgaaaaag cctatgacct gctgactctg gcgcagggtg agttccaggc
    14401 cgatactgcc gttgctggtt tgcaggcagc ttatgctcgc gacgaaaacg acgagttcgt
    14461 gaaagcgacc gttattcgtg cagaaggtca gccagatgcg gcaatggaag acggcgatgc
    14521 gctgattttc atgaacttcc gtgctgaccg cgcgcgtgaa atcactcgtg cattcgtgaa
    14581 cgctgatttc gatggcttcg cgcgtaagaa agtggttaac gtcgatttcg tgatgctgac
    14641 cgaatacgcc gctgacatca aaactgcggt tgcttaccca cccgcttccc tggttaacac
    14701 cttcggcgag tggatggcga aaaacgacaa aactcagttg cgtatttccg aaaccgaaaa
    14761 atatgcccac gttactttct tcttcaacgg tggcgtagaa gagtcgttca aaggcgaaga
    14821 tcgcattctg atcaactcac cgaaagtggc tacctacgat ctgcaaccgg aaatgagctc
    14881 cgcagagctg accgaaaaac tggttgcggc catcaagagc ggcaaatacg acaccattat
    14941 ctgtaactat ccgaacggcg acatggtagg tcacaccggg gtgatggaag cggcggttaa
    15001 agcggttgaa gcactggatc actgcgtgga agaagtcgcg aaagcggttg agtccgtggg
    15061 tggacaactg ctgatcaccg ctgaccacgg taacgctgaa cagatgcgcg atccggcaac
    15121 gggtcaggca cacacggcac acaccaacct gccagttccg ctgatttacg ttggtgataa
    15181 gaacgtgaaa gcggttgcag gcggcaaact ttctgacatc gcgccgacca tgttgtcgct
    15241 gatgggtatg gaaatcccgc aagagatgac tggtaagccg ctgttcatcg tggaataatc
    15301 cctccccatg aggggaaagg cgattaatac catgacacgg gccgtgaaac cgcgcaggtt
    15361 tgcaatcagg cccatcatct acgccagcgt tcttagcgct ggcgtattgt tgtgcgcctt
    15421 ttccgcccac gcggatgagc gtgaccaact caaatctatt caggccgata tcgccgcaaa
    15481 agagcgcgcg gtacgccaaa agcaacaaca acgcgcaagc ctgctcgcac aattgaaaaa
    15541 gcaggaagaa gcgatctctg aagccacccg taagctgcgc gaaacgcaaa acacgctcaa
    15601 tcaactcaat aaacagattg atgagatgaa cgcgtcgatt gccaaactgg agcagcaaaa
    15661 agccgcccag gagcgcagcc tcgccgcaca actggatgcc gcattccgtc agggcgagca
    15721 taccggtatt cagctaattc tcagcggtga agaaagccag cgtggacagc gtttacaggc
    15781 ttatttcggc tatctcaacc aggcgcgaca agaaaccatt gcccagttga agcaaacgcg
    15841 tgaagaagtc accatgcagc atgctgaact ggaagagaaa caaagcgagc aacaaacgct
    15901 gttatatgaa cagcgcgccc aacaggcgaa actgactcag gctctgaacg agcgtaaaaa
    15961 gacgctggcg gggctggaat cttccatcca gcaaggtcag cagcagctaa gtgaactgcg
    16021 tgccaacgaa tcccgtctgc gtaacagcat tgcccgtgct gaagccgcgg cgaaagcgcg
    16081 tgctgaacga gaagcgcgtg aggcccaggc ggttcgcgag cgccagaaag aagcgacgcg
    16141 caaaggcacc acctacaaac cgaccgaaag cgaaaaatcg ctgatgtccc gaactggtgg
    16201 cctgggtgcg ccgcgtggtc aggcattctg gccggttcgc gggccgacgc tgcatcgcta
    16261 tggcgagcag ctacagggtg aattacgctg gaaagggatg gttatcggtg cctctgaagg
    16321 tactgaagtt aaagcaattg ccgacggtcg agtgattctg gctgactggc tgcaaggtta
    16381 cggtctggtg gtggtggttg agcacggtaa aggcgacatg agtctttacg
//
LOCUS       DAJTUP010000086        16376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTUP010000086 DAJTUP010000000
VERSION     DAJTUP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.086"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..1090
                     /gene="fepE"
                     /locus_tag="OO468_002923"
                     /pseudo
     CDS             1..1090
                     /gene="fepE"
                     /locus_tag="OO468_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
     gene            complement(1087..1902)
                     /gene="fepC"
                     /locus_tag="OO468_002924"
     CDS             complement(1087..1902)
                     /gene="fepC"
                     /locus_tag="OO468_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6259964.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(1899..2891)
                     /gene="fepG"
                     /locus_tag="OO468_002925"
     CDS             complement(1899..2891)
                     /gene="fepG"
                     /locus_tag="OO468_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR6259965.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            complement(2888..3892)
                     /gene="fepD"
                     /locus_tag="OO468_002926"
     CDS             complement(2888..3892)
                     /gene="fepD"
                     /locus_tag="OO468_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR6259966.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            4003..5235
                     /gene="entS"
                     /locus_tag="OO468_002927"
     CDS             4003..5235
                     /gene="entS"
                     /locus_tag="OO468_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR6259967.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            complement(5239..6195)
                     /gene="fepB"
                     /locus_tag="OO468_002928"
     CDS             complement(5239..6195)
                     /gene="fepB"
                     /locus_tag="OO468_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR6259968.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            6487..7662
                     /gene="entC"
                     /locus_tag="OO468_002929"
                     /pseudo
     CDS             6487..7662
                     /gene="entC"
                     /locus_tag="OO468_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            7672..9282
                     /gene="entE"
                     /locus_tag="OO468_002930"
     CDS             7672..9282
                     /gene="entE"
                     /locus_tag="OO468_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR6259969.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            9296..10153
                     /gene="entB"
                     /locus_tag="OO468_002931"
     CDS             9296..10153
                     /gene="entB"
                     /locus_tag="OO468_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR6259970.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            10153..10899
                     /gene="entA"
                     /locus_tag="OO468_002932"
     CDS             10153..10899
                     /gene="entA"
                     /locus_tag="OO468_002932"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR6259971.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            10902..11315
                     /gene="entH"
                     /locus_tag="OO468_002933"
     CDS             10902..11315
                     /gene="entH"
                     /locus_tag="OO468_002933"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR6259972.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            11496..13601
                     /gene="cstA"
                     /locus_tag="OO468_002934"
     CDS             11496..13601
                     /gene="cstA"
                     /locus_tag="OO468_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR6259973.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            13783..13980
                     /locus_tag="OO468_002935"
     CDS             13783..13980
                     /locus_tag="OO468_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR6259974.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(13990..15078)
                     /gene="hcxA"
                     /locus_tag="OO468_002936"
     CDS             complement(13990..15078)
                     /gene="hcxA"
                     /locus_tag="OO468_002936"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR6259975.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            15187..16347
                     /gene="ybdL"
                     /locus_tag="OO468_002937"
     CDS             15187..16347
                     /gene="ybdL"
                     /locus_tag="OO468_002937"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR6259976.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
BASE COUNT         3611 a         4294 c         4710 g         3761 t
ORIGIN      
        1 tgagtatcct ctggcgtcgc ccagtaataa tgaaattgat ttacttaatc taatcgaagt
       61 tttatggcgg gccaaaaaaa cggtcatggc ggtcgttttt gcatttgcct gcgcaggttt
      121 actgatctct ttcatcctgc cgcaaaaatg gaccagctca gccgttatta ctcccgccga
      181 agccattcag tggcaggacc tggagaaaac ctttaccaaa ctccgcgttc ttgatctcga
      241 tgttaatatc gaccgtggcg gtgcatttaa cctgtttatc aagaagtttc agtctgttag
      301 cttgctggaa gagtacctgc gttcatcacc ttatgtgatg gactaattaa aagaggcgaa
      361 aatcgatgaa ctggatttgc atcgcgctat tgttgcattg agcgaaaaaa tgaaagcggt
      421 tgatgacaat gctagtaaga aaaaagatga accgtcactg tatacatcct ggacgctaag
      481 ttttaccgcg ccaaccagta aagaggcgca gaccgttttg agcgggtata tcgattatat
      541 ctctgcgttg gtggtgaaag agtcgataga aaacgtccgt aataaactgg agatcaaaac
      601 ccagtttgaa aaagaaaaac tggctcagga tcgcattaaa acgaaaaatc aacttgatgc
      661 aaacattcag cgcctcaatt attcactcga cattgccaac gcggcaggaa ttaaaaagcc
      721 cgtatacagt aatggtcagg ccgttaaaga tgaccccgat ttctctattt ccctcggcgc
      781 agacggtatt gaacgcaaac tggaaattga aaaagcggtt actgacgttg cggaactgaa
      841 cggtgaatta cgtaatcggc agtatcttgt cgagcaatta acaaaagcaa atgtcaacga
      901 tgtgaatttt acgccgttta aatatcagtt aagcccgtca ttgccagtga aaaaagacgg
      961 tccgggtaag gcgattattg tgatcctttc cgcgctgatc ggcgggatgg tggcttgtgg
     1021 tagcgtgtta ttgcgctatg cgatggcatc cagaaaacag gatgccatga tggcagacca
     1081 cttagtttaa ctatttgcgg ttgaaggtgc cgttcgtccg agcggcacca caagcggcat
     1141 tccggctacc ggatcgtcaa tgatcatgca tcgcagacca taaatgcgct caatcagttc
     1201 agcagtgaca atctccttcg gcgctccctg agcaacaatt ttcccttccc gcaatgcaat
     1261 caaatggctg gcgtaacgac aggcctgatt aagatcgtgc agcaccgccg ccagggtata
     1321 gcctttctcg cggttcagtt cgcttaacag ttccagtaga tcaatctgat gactgatatc
     1381 cagccaggtc gtgggttcgt cgagcagcat aattgccgtt tcctgggcca gcaccatcgc
     1441 gatccacgct cgctggcgtt gtccgccaga aagggtatcc acgctttgat ctgccagatg
     1501 agttattccc gtggcctgca tcgctttcgt taccgcttct tcatcctctt tgcgccagcg
     1561 ggtaaacagc ggttgatgcg gataacgtcc acgcgccacc agctcctgca cggtgatatc
     1621 gcccggcgtg gtagcatttt gcgccaacag accaatccgg cgtgcaactt ctttactggc
     1681 gtaatgttga atgtgctcgc catccagcca gacatgcccg tgagcaggcg tcatcaggcg
     1741 gcttaaggta cgcagtaacg tggatttacc acagccattt ggtccgataa ttgccgtgaa
     1801 gtgaccatca ggaatttcta cagtcagatt ttccgcaacg gtatatttgc catatcccag
     1861 ggttaactgt tcgccgcgca aacgggctac tgattcggtc attttttgcg agactcctga
     1921 attaacaaga cgataaggta aataccgccg aggctgacgg taacgacgcc aaccggaagt
     1981 tgatacggca taaacagttg ttgggcgcag agaacggccg ccagcagtaa cagcgctccg
     2041 cacagcgccg cctgggtcag cccccagcga gcggtgccgc taatgcgtcg ggcaatgtgc
     2101 ggcgcgacta aagcaataaa ggaaatcggc ccggcaagcg ccgttgccgc agcggtaagc
     2161 accactgcaa ccagcatcat taacagacgc gaacgctcga cgctgacgcc cagcgcgcag
     2221 gcggtatcat cgcccatctc cagcaagcgc attcgccgca ccagcaaggt ggcggcaatg
     2281 agcatcaata taatgatggg tgcggaaggc gaggtttttg cccacgtcag gccgttgagc
     2341 gatccggcat tccacaaacc tgctgttagc gccgtttcta aagacgcttt cagcaacagc
     2401 caggtattaa aggccaccag catggcgcga acgccgatac cgataataat caaccgaaag
     2461 gtgtcgatac cgttacgcca ggcgagcagc cagaccaaca gcgaagtgat aatgccgccc
     2521 accattgctg ccagcgcgat agccgtcaga tcctgaccaa acagcaccat cgccaccagc
     2581 acgccgctcc acgccccggt gttaaagccc attacgtcag ggctgccgag cgggttacgc
     2641 atcagcgact gaaaaatcgc gccgctgacg cccagcgccg cgccaatcaa cagcgccatc
     2701 agcacgcgtg gtaaacgcca ttcggtgacc accatcgtca tactgcgcgg cgcatcgccc
     2761 atcaacgcgg cgaatacctg cgagatttcc agcgtgacgg caccgctgcg taatccccag
     2821 atacctgcaa ccacacaggc ggaaatcagc agcaaacagg tgatgagtaa tcggcgagag
     2881 acgtaaatca tgcaccacct cgcgttttac gtcgcacgag gaagatcagc accggtgcac
     2941 caataaacgc actgaccaca gaaacgcgca gttcgccggg cacaatcacc cgcccgatga
     3001 tatcggcaaa cagcagcagg gcaggggtag caagtagcgt gacgggcagc gaccagcgat
     3061 gatcggcacc taccagccag cgcgccatgt gcggcatcat caggccgata aaggcaatcg
     3121 ggccaactac cgccgtcgca ctaccacaaa gcacggtaat cgccagcaga ccaatcaact
     3181 gtgtgcgcgc cacgcgactg cccagcgccg tcgcggtgtc actgccgagg ctcaaactgt
     3241 tcagcgcgcg actcagtaat agcgcagttg ctccggcgat cagaaccggg atcagcacca
     3301 cttttaaggt atgtagattg cgaatatcca gcgaaccggc ttgccagaaa cgcagttgat
     3361 cgtagacgtc aggattaagc agggcgatgc cgctggtcag tccttccagc accgccgcca
     3421 gcgccacgcc cgccagggtt aaacgcaccg gacttaactg ccctccaccc tgactgccgg
     3481 taaaggcaac aatcaatgag gccaccagcg ccccggcgaa ggccatcgcc agttgttcct
     3541 gtgcggatga gtaaccaaac agcgccgcac ccagcacaat ggcaaagctg gctccggcgt
     3601 tcacgccaag caagccgggg tcggcaagtg ggtttcgggt gagggtttgc attaacgccc
     3661 cggcaaggcc aagcgcgccg cctgccagta aaccggcaag ggtacgcggc agccgcgcgt
     3721 cgagcacgat ggtgcagtcg gcgctctggc aggtgccgga gaaggcctcc agcactacgg
     3781 aagcggggag tgattttgcc ccaatgagca ggcttaatgc cgtcgcgata atcagtaata
     3841 acagcaatcc gggcacggca atggcgcgtg tcacggcaac agaaccagat atacgaactt
     3901 ccatgataat gaaattaatt atcgttatcg atcttatttg gatatgttaa catgtgcagc
     3961 ctaagaatag gtatttaaaa tatttgatgg caaggcattg taatgaataa acaatcctgg
     4021 ctgcttaacc tcagcctgtt gaaaacgcac ccggcgtttc gcgcagtatt cctcgctcgt
     4081 tttatctcaa ttgtgtctct gggtttgctc ggcgtcgcgg tgccggtgca gatccagatg
     4141 atgacccatt ctacctggca ggtggggctt tcggtgacgc tgaccggcgg cgcgatgttt
     4201 gttggcctga tggttggcgg tgtgctggcg gatcgctatg aacgcaaaaa agtgattttg
     4261 ctggcgcgcg gcacctgtgg cattggcttc attggactgt gccttaatgc actgctgccg
     4321 gagccgtcat tgctggcaat ctatttactt ggtttatggg atggtttttt cgcatcactt
     4381 ggtgttacgg cgctactggc ggcaacatct gcactggtag ggcgtgaaaa cttaatgcag
     4441 gccggggcga tcaccatgtt gaccgtgcgc ctggggtcgg tgatttcgcc catgattggc
     4501 ggtttattgc tggcgaccgg cggcgtagcc tggaactacg ggctggcggc ggcgggcacg
     4561 tttattacct tgttaccgtt attaagcctt ccggagttgc caccgccacc gcagccgctt
     4621 gagcatccgt tgaaatcatt actggcagga tttcgttttc tgcttgccag cccgctgctg
     4681 ggtggtttat tgacgatggc gagcgcggtg ctggtactgt atccggcgct ggctgacaac
     4741 tggcagatgt cagcggcaca gattggtttt ctctacgcgg cgatcccgct cggcgcggct
     4801 attggtgcgt taaccagcgg gaagctggca catagtgcgc gaccagggtt attgatgctg
     4861 ctctccacgc tgggatcgtt cctcgccatt ggtctgtttg gcctgatgcc gatgtggatt
     4921 ttaggcgtgg tttgtctggc gctgttcggc tggctgagcg cggttagttc gttgctgcaa
     4981 tatacaatgc tgcaaacgca aaccccggaa gcgatgttag ggcggattaa cggtttgtgg
     5041 acggcgcaga acgtgacggg cgatgccata ggcgcggcgc tgctgggtgg tttgggcgcg
     5101 atgatgacac cggttgcttc cgcaagcgcg agcggttttg gtttgttgat tatcggcgtg
     5161 ttgttattgc tggtgctggt ggagttgcga cgttttcgcc agacgccgcc gcaggtgaca
     5221 gcgtccgaca gttaatgctt aaaacagcgc cttaagccgt tccagcactt gcatggcgct
     5281 gtagtaatcc aggcggaacg tctcggttcc cagcgcataa acctgcttgt tttgtaccgc
     5341 aggcaggtgc gcgagcagtg gattggcata aatcgcatcg gcatctttct gatcaccagc
     5401 gaacaggaat agcgattcac cgtttaaccc cgcagccaga ttttccccac caagctgaat
     5461 gatgtcatgg cgtttgccct ggctttggct ggcgtttaac cctgcgggca atttcgccag
     5521 cgtaaagccg agttgttcca gcatctgccc ttgtgctgat tccggcgtcc agagattggc
     5581 actgtgtgca gcggcggtat agacaatggc agtgaccggc tgcggcggta atttgatttg
     5641 ctctttcgcc gctgccagtt gcttatcaaa ctgtgcaatc cgctctgccg cttgtttctc
     5701 atgcccggta atttcgccaa gctgcgttaa cagcgactgc cagcttttgt cgtcgtaatt
     5761 gatgattaat gtcggggcga tggtggaaag ctgatcatac agcgccagcg ccgaatcccc
     5821 gccggttgcg ctaattaaaa tcagatccgg catttgcgcg gcaacggctt cggcgctcgg
     5881 ttcgccgata tagagccgtt gcagtttgcg ttctttcgcc accttgctcc actggcgtaa
     5941 aaagccctgg tcatctgcga cgcggttatt cggcgtggtc gcgccgctgg cgatcaccgg
     6001 agcatcaatc gccagcagtg atccggtcag ggtgacgctg gtggaaacaa tacgctgcgg
     6061 ctggctttcc agggtatgag tgccccggct gtcagtaatc tgacgcggcc agtcggcggc
     6121 ctgaactacg gctattcctg aaagcaaaag tcctgttaat agaagggcgt tgcggtagag
     6181 cggggcgagt ctcacaagtc agcttcctgt tattaataag gttaagggcg taatgacaaa
     6241 ttcgacaaag cgcacaatcc gtccctttgg ggagaggggg caaaacggcg caggacatca
     6301 cattgcgctt atgcgaattc atcaataatg cttctcattt tcattgtaac cacaactaga
     6361 tgcaaccccg agttgcagat tgcgttacct caagagttga catagtgcgc gtttgctttt
     6421 aggttagcgg ccgaaaatat aagtgataat cattattaaa gcctttatca ttttgtggag
     6481 gatgatatgg atacgtcact ggctgaggaa gtacagcaga ccatggcaac acttgcgccc
     6541 aatcgctttt tctttatgtc gccgtaccgc agttttacga cgtcaggatg tttcgcccgt
     6601 ttcgatgaac cggctgtgaa cggggattcg cccgacagtc ccttccagca aaaactcgcc
     6661 gcgctgtttg ccgatgccaa agcgcagggc atcaaaaatc cggtgatggt cggggcgatt
     6721 cccttcgatc cacgtcagcc ttcgtcgctg tatattcccg aatcctagca gtcgttctcc
     6781 cgtcaggaaa aacagacctc agcccgctgt tttacccgca gccagtcgct gaacgtggtg
     6841 gaacgctagg caattcctga acaaaccacg tttgaacaga tgggtgcccg cgccgccgca
     6901 cttaccgcca cgccgcaggt cgacaaagtg gtgttgtcac gattgattga tatcaccact
     6961 gacgccgcca ttgatagtgg cgtattgctg gaacggttga ttgcgcaaaa cccggttagt
     7021 tacaacttcc atgtcccgct ggctgatggt ggcgtcctgc tgggggccag cccggaactg
     7081 ctgctacgta aagacggcga gcgttttagc tccattccgt tagccggttc cgcgcgtcgt
     7141 cagccggatg aagtgctcga tcgcgaagcg ggtaatcgtc tgctggcgtc agaaaaagat
     7201 cgccatgaac atgaactggg gactcaggcg atgaaagagg tactgcgcga acgcagtagt
     7261 gagttacacg ttccctcctc tccacaattg attaccacgc cgacgctgtg gcatctcgca
     7321 actccctttg aaggtaaagc gaattcgcaa gaaaacgcac tgactctggc ctgtctgctg
     7381 catccgaccc ccgcgctgag cggtttcccg catcaggccg cgacccaggt tattgctgaa
     7441 ctggagccgt tcgaccgcga actgtttggc ggcattgtgg gttggtgtga cagcgaaggt
     7501 aacggcgaat gggtggtgac catccgctgc gcgaagctgc gggaaaatca ggtgcgtctg
     7561 tttgccggag cggggattgt gcctgcgtcg tcaccgttgg gtgagtggcg cgaaacaggc
     7621 gtcaaacttt ctaccatgtt gaacgttttt ggattgcatt aaggagcgag gatgagcatt
     7681 ccattcaccc gctggccgga agagtttgcc cgtcgctatc gggaaaaagg ctactggcag
     7741 gatttgccac tgaccgacat tctgactcgc cacgctgcga gtgacagcat cgcggttatc
     7801 gacggcgagc ggcagttgag ttatcgggag ctgaatcagg cggcggataa cctcgcctgt
     7861 agtttgcgcc gtcagggcat taaacctggt gaaaccgcgc tggtacaact gggtaacgtc
     7921 gctgaacttt acattacctt tttcgcgctg ctgaaactgg gcgtcgcgcc ggtgctggcg
     7981 ctgttcagcc atcagcgtag tgaactgaac gcctacgcca gccagattga accggcgtta
     8041 ctgattgccg atcgccagca tgcgctattt agcggggatg atttcctcaa cacatttgtt
     8101 gcagagcatt cttccattcg cgtggtgcaa ctgcacaacg acagcggtga gcataacttg
     8161 caggatgcga ttaaccatcc ggcagagggt tttactgcca cgccatcacc tgctgatgaa
     8221 gtggcctatt tccagctttc cggcggcacc accggcacac cgaaactgat cccgcgcact
     8281 cataacgact attactacag cgtgcgtcgt agcgccgaga tttgtcagtt cacacaacag
     8341 acgcgctacc tgtgctcgat cccggcggct cataactaca ctatgagttc gccaggatcg
     8401 ctgggcgtct ttcttgccgg aggaacggtt gttctggcgg ccgatcccag cgccacgctt
     8461 tgcttcccgt tgattgcaaa acatcaggtt aacgttaccg cgctggtgcc gccggcagtc
     8521 agcctgtggt tgcaggcgct ggctgaaggc gaaagccggg cgcagcttgc ctcgctgaaa
     8581 ctgttacagg tcggcggcgc acgtctttct gccacgcttg cagcgcgtat tcccgctgag
     8641 attggctgcc agttgcagca ggtgtttggc atggcggaag ggctggtgaa ctacacccgt
     8701 cttgatgata gcgcggagaa aattatccat acccagggtt acccaatgtg tccggacgat
     8761 gaagtgtggg ttgccgatgc cgaaggaaat ccactgccgc aaggggaagt tggacgcctg
     8821 atgacgcgcg ggccgtacac cttccgtggc tattacaaaa gtccgcagca caatgccagt
     8881 gcctttgatg ccaacggttt ttactgttcc ggcgatctga tctctattga tccagagggt
     8941 tacatcaccg tgcaggggcg cgagaaagat cagatcaacc gtggcggcga gaagatcgct
     9001 gccgaagata tcgaaaacct gctgctgagc catccggcag tgatctatgc cgcactggtg
     9061 agcatggaaa atgagctgat gggcgaaaaa agctgtgctt atctggtggt aaaagagccg
     9121 ctgcgcgcgg tgcaggtgcg tcgtttcctg cgtgaacagg gtattgccga atttaaatta
     9181 ccggatcgcg tggagtgtgt ggattcactt ccgctgacgg cggtcgggaa agtcgataaa
     9241 aaacaattac gtcagtggct ggcgtcacgc gcatcagcct gaaggagaga acacgatggc
     9301 tattccaaaa ttacaggctt acgcactgcc ggagtctcac gatattccgc agaataaagt
     9361 tgactgggcc tttgaaccgc aacgtgccgc gttgttaatc catgatatgc aggactattt
     9421 tgtcagcttc tggggcgaga actgcccgat gatggagcag gtgatcgcga atattgctgc
     9481 gctgcgcgac tactgcaaac agcacaatat cccggtttat tacaccgccc agccgaaaga
     9541 gcagagcgat gaagatcggg cgctgttgaa tgatatgtgg gggccgggcc tgacccgctc
     9601 gccggaacag caaaaggtgg tggatcgcct gacgccagat gccgacgaca cggtgctggt
     9661 gaagtggcgc tacagcgcgt ttcatcgttc tccgctggag caaatgctca aagagagtgg
     9721 acgtaaccag ctgattatta caggagtata tgcccacatt ggctgtatga ccaccgcaac
     9781 cgacgcattt atgcgcgata ttaaaccgtt tatggtggcg gatgcgctgg ctgatttcag
     9841 ccgtgacgag catttgatgt cgctgaaata tgtggccgga cgttctggcc gggtggtgat
     9901 gaccgaagaa ttactgccag cacctatccc cgccagcaaa gcggcgctgc gtgaggtgat
     9961 cctgccgttg ctggacgagt ccgatgaacc gttcgatgac gacaacctga tcgactacgg
    10021 tctggattcg gtgcgcatga tggcgctggc ggcgcgctgg cgcaaagtgc atggtgatat
    10081 cgactttgtc atgctggcga aaaacccgac catcgacgcc tggtggaagc tactctcccg
    10141 cgaggtgaaa taatggattt cagcggtaaa aatgtctggg taaccggcgc aggtaaaggt
    10201 atcggctacg ccacggcgct gacgtttgtt gaggcgggag cgaaagttac aggttttgat
    10261 caagcgttca ctcaggagca atatcccttt gcgaccgaag taatggatgt tgccgacgcc
    10321 gggcaggtgg cgcaggtatg tcagcgactg ttagctgaaa cggagcgact ggacgtgctg
    10381 atcaatgcgg cgggaatttt gcgcatgggc gcgaccgatc agctcagtaa agaggactgg
    10441 cagcagactt ttgcggttaa cgtcggcggt gcgtttaacc tgttccagca aaccatgaac
    10501 cagtttcgcc gtcagcgggg cggggcgatt gtcactgtgg cgtccgacgc cgcacacacg
    10561 gcgcgtattg gtatgagtgc ttatggcgca tcgaaagcgg cgctgaaaag cctggcgttg
    10621 agcgtcgggc tggaactggc gggtagcggc gtgcgctgta atgtggtttc gcctggctcc
    10681 accgacaccg atatgcaacg cacgctgtgg gtgagcgatg acgccgaaga acagcgtatt
    10741 cgcggctttg gcgagcagtt taaactcggc attccgctgg ggaaaatcgc ccgtccacaa
    10801 gagatcgcca acacgatttt gttcctcgcc tctgacctcg ccagccatat caccctacag
    10861 gatattgtgg tcgatggcgg ctcaacgctg ggggcataag catgatctgg aaacgccatt
    10921 taacgctcga cgaactgaac gccaccagcg ataacacaat ggtggcgcat ctgggaattg
    10981 tgtataccct tctgggagat gatgtgctgg aagccgaaat gccggttgat acccgtactc
    11041 atcagccgtt tggcctgctg catggcggcg cgtcggcggc gctggctgaa acgctgggat
    11101 cgatggccgg atttatgatg acccgtgacg ggcagtgtgt ggtgggcacg gaacttaacg
    11161 ccacccatca tcgcccggtg tctgaaggca aggtacgcgg cgtctgccag ccgctgcatc
    11221 ttgggaggca aaatcagagt tgggaaatcg tcgttttcga tgaacagggg cggcgttgct
    11281 gcacttgtcg gctgggtacg gcagttttgg gatgagcccg acagaattaa atgagattgc
    11341 aggaaaactc ggttaacgga gtgatcgagt taacattgtt aagttaaata ttggtttcaa
    11401 ctccgattta catggttacc gtgttgttaa atcgtacaaa gatgttatag aaacaaaatg
    11461 taacatctct atggacacgc acacggataa caactatgaa caaatcaggg aaatacctcg
    11521 tctggacagt gctctctgta atgggagcat ttgctctggg atacattgct ttaaatcgtg
    11581 gggaacagat caacgcgctg tggattgtgg tggcgtcggt ctgtatctat ctgatcgctt
    11641 accgttttta tgggctgtat atcgccaaaa atgtgctggc ggttgacccg acgcgtatga
    11701 cgccagcggt gcgccataac gacgggctgg actatgtgcc gacggacaag aaagtgctgt
    11761 tcggtcacca ttttgcggcc attgccggag cagggccgct ggtggggccg gtgctggcag
    11821 cgcaaatggg ctacctgccg gggatgatct ggctgctcgc cggggtggtt ctcgccggtg
    11881 cggtgcagga tttcatggtg ctgtttgttt ctacgcgccg tgacggtcgc tcgctgggtg
    11941 agctggtcaa agaagagatg gggccaactg ccggggtgat tgcgctggta gcctgcttta
    12001 tgatcatggt cattatcctt gcagtactgg cgatgatcgt ggtgaaagcc ctgactcata
    12061 gcccgtgggg aacgtatacc gttgcgttca ccattccgct ggcgattttt atgggaatct
    12121 acctgcgcta tctgcgtccg ggccgcattg gcgaagtgtc agtgattggc ctggtgttcc
    12181 tgattttcgc cattatctct ggcggctggg tggcagaaag tccgacctgg gcaccgtact
    12241 ttgactttac cggcgtgcag ctgacctgga tgctggtggg ctacggtttt gtggcggcgg
    12301 tgctgccggt gtggttgctg ctggccccgc gtgactatct ctcgaccttc ctgaaaatcg
    12361 ggactatcgt cggtctggcg gtaggcattt tgattatgcg cccgacgctg accatgcctg
    12421 cgctgaccaa atttgtcgat ggcactggcc cggtatggac cggtaacctg ttcccgttcc
    12481 tgtttatcac catcgcctgt ggtgcggtgt ctggcttcca tgcgctgatc tcttccggca
    12541 ccacgccgaa gatgctggcg aacgaagggc aggcgtgctt tatcggctac ggtgggatgt
    12601 tgatggaatc cttcgtggcg attatggcgc tagtttctgc ctgtatcatc gatccgggcg
    12661 tgtattttgc catgaacagc ccaatggcgg tgctggctcc ggcagggacg gcggatgtag
    12721 tcgcttctgc cgcgcaggtg gtgagtagct ggggctttag cattacacca gatacgctca
    12781 accagattgc cagcgaagtg ggtgaacagt cgatcatttc ccgtgcgggc ggtgcgccga
    12841 cgttggcggt ggggatggct tacatcctgc acggtgcgct gggtggtatg atggatgtgg
    12901 cgttctggta tcacttcgcc attctgtttg aagctctgtt tattctgacg gcggtagatg
    12961 cgggtacgcg tgctgcgcgc tttatgttgc aggatctgct gggcgtggtg tctcctggcc
    13021 tgaagcggac cgattcactg cctgctaacc tgctggcaac agcgctgtgc gtactggcgt
    13081 ggggctactt cctccatcag agcgtggtcg atccgctggg cggtattaac actctgtggc
    13141 cgctgtttgg tattgccaac cagatgctgg cagggatggc gctgatgctc tgtgccgtgg
    13201 tgttgttcaa gatgaaacgt caacgttacg cctgggtggc gctggtacca acggcctggc
    13261 tgctgatttg taccctgacc gctgactggc tgaaagcgtt tagcccggat gcgaaagtcg
    13321 gcttcctggt cattgctaac aagttccagg caatgatcga cagcggcaat attccgtcgc
    13381 agtatactga gtcacagctg gcgcaactgg tgttcaacaa ccgtctggat gccgggttaa
    13441 ccatcttctt tatggtggtc gtggtggtcc tggcactgtt ctcgattaag acggcacttg
    13501 cggcattgaa agatccgaag ccaacggcga aagaaacgcc gtatgagccg atgccggaaa
    13561 atgtcgagga gatcgtagcg caggcgaaag gcgcgcacta aagtcagagt gaggggggat
    13621 gttggcgaat gttggcatag tgccgagggt tccccctcac cctaaccctc tccccgatgg
    13681 ggcgagggga ctgaccgagt gcgttgatag catttgtagg ccggataaag cgttcacgcc
    13741 gcatccggca ctctttcagc aacatggtta gcggaggcca agatgtttga ttcactggca
    13801 aaagccggaa aatatttagg tcaggcggcg aagctgatga ttggtatgcc tgattacgac
    13861 aactatgtcg aacatatgcg ggttaatcat cccgatcaaa cgccgatgac ctacgaggag
    13921 tttttccgtg agcggcagga cgcgcgctac ggtggaaaag gcggcgcgcg ctgctgctaa
    13981 atttgcacgt caggctttaa acgattccac tttttcgaac gctgcacgca acgtatctgg
    14041 tgtcagcgtg actggcaggt aatgaatgga ctccaccggg cgcaaggtgt gggcaatcac
    14101 tttgtcgatc tccgcctgat tattgatatc cacctccagc tccgccagcg tagtcggcag
    14161 atgaaaacgc tgatacgccc cggttaattg cgccagcaca tcatcctgac ccagcaaggc
    14221 gctttgcacc agaattccgt aagcgacttt ggtgccgtga agaaacttct cggtttgcgg
    14281 caggacagtc agaccgttat gcacggcatg agccgccgcc acacgcgtaa aacgatcgcc
    14341 cagaccaccg accatcccac caccagcaat aatagcatcc accacatcgc aaaatgattg
    14401 cgttaactgc tgattttgct gatcggcaag tgcctgttcg ctactgttta acaagacgtc
    14461 gcgaatggcc tgcgcattat tgatccccag ccgcacggtt agcggcaacg tttctggttg
    14521 cggagccagc accaccgctt cataccattt cgccagcgtg tcaccgatcc ccgccagcag
    14581 atattgttgc ggtgcattga ggataatctc cggttccacc agcaccataa aattggcgtc
    14641 gtcgaaaatc tcataatgca gcgcctgtcc ggcatcgtta taccagacgg agagcggtgt
    14701 ccaggctgcg caggtggcgg cgatcgtcgg agcggcaaca aatggcagac cgagacggcg
    14761 ggcgagggct ttcgcggtgt cgagcagtgc gccgccgccg acgccaatca ccacgctgcg
    14821 atcgtcaccg gactcagccg ccagttgctg tacatcgctt tcgctgcaat gaccacgaaa
    14881 caaaatatgc tttgcccctg gaagtccaaa cgctggcgga agtttggttt gcgccgcagc
    14941 aatggcgcgt ttgccgtaga tccacaccgc gcgagaaagt tgttcatcag tgaaaaaatt
    15001 gtgcaggtga ttgaaacttc ctggatgtga aaagtagtta gccgggccga cgaccacgcg
    15061 gataggattg tgaggcatgt tgtgttgtcc ttattgtttt atttagacat ctaaacgtct
    15121 tgattgccaa atactggcat cgtgttatag tgccttcaac acgcaacttc gtcaggtaca
    15181 ataaaaatga caaataaccc tctgattcca caaagcaaac ttccacaact tggcaccact
    15241 attttcaccc agatgagcgc gctggcgcag caacaccagg cgattaacct gtcgcaaggc
    15301 tttcctgatt ttgatggtcc gcgctattta caggagcggc tggcgtacca cgttgcccag
    15361 ggggcaaacc aatacgcgcc catgaccggc gtgcaggcct tgcgcgaggc gattgctcag
    15421 aaaacggaac gtttgtatgg ctatcaacca gatgccgata gcgatatcac cgtaacggca
    15481 ggggcgacgg aagcgttata cgcggcgatt accgcactgg tgcgcaatgg cgatgaagtg
    15541 atttgttttg atcccagcta tgacagttac gcccccgcca tcgcgctttc tgggggaata
    15601 gtgaaacgta tggcactgca accaccgcat tttcgcgttg actggcagga atttgccgca
    15661 ttattaagcg agcgcaccag actggtgatc ctcaacactc cgcataaccc cagtgcaact
    15721 gtctggcagc aggctgattt cgccgctttg tggcaggcga tcgccgggca cgagattttt
    15781 gtcattagcg atgaagtcta cgagcacatc aacttttcac aacagggcca tgccagtgtg
    15841 ctggcgcatc cgcagctgcg tgagcgggca gtggcggttt cttcatttgg caagacctat
    15901 catatgaccg gctggaaagt gggttattgt gttgcgccag cgcccatcag cgccgaaatt
    15961 cgcaaggtac atcagtatct gaccttttcg gtgaataccc cggcacagct ggcgcttgct
    16021 gatatgctac gtgcagaacc tgagcattat cttgcgttac cggactttta tcgccagaag
    16081 cgcgatattc tggtgaatgc tttaaatgaa agccggctgg agattttacc gtgtgaaggt
    16141 acatactttt tgctggtgga ttacagcgcg gtttctaccc tggatgatgt tgagttttgc
    16201 cagtggctga cgcaggagca cggcgtagcg gcgattccgc tgtcggtgtt ttgcgccgat
    16261 cccttcccac ataaactgat tcgtctctgt tttgccaaga aggaatcgac gttgctggca
    16321 gcagctgaac gcctgcgcca gctttagcta tttaaccgtc caggcgcggg aatatt
//
LOCUS       DAJTUP010000087        16294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTUP010000087 DAJTUP010000000
VERSION     DAJTUP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.087"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(2..1978)
                     /gene="speA"
                     /locus_tag="OO468_002938"
     CDS             complement(2..1978)
                     /gene="speA"
                     /locus_tag="OO468_002938"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR6259977.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(1987..2118)
                     /gene="yqgB"
                     /locus_tag="OO468_002939"
     CDS             complement(1987..2118)
                     /gene="yqgB"
                     /locus_tag="OO468_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR6259978.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2254..2469
                     /locus_tag="OO468_002940"
     CDS             2254..2469
                     /locus_tag="OO468_002940"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6259979.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2773..3927
                     /gene="metK"
                     /locus_tag="OO468_002941"
     CDS             2773..3927
                     /gene="metK"
                     /locus_tag="OO468_002941"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR6259980.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4364..5758
                     /gene="galP"
                     /locus_tag="OO468_002942"
     CDS             4364..5758
                     /gene="galP"
                     /locus_tag="OO468_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR6259981.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5835..6332
                     /locus_tag="OO468_002943"
     CDS             5835..6332
                     /locus_tag="OO468_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR6259982.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6427..7134
                     /gene="endA"
                     /locus_tag="OO468_002944"
     CDS             6427..7134
                     /gene="endA"
                     /locus_tag="OO468_002944"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR6259983.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7214..7945
                     /gene="rsmE"
                     /locus_tag="OO468_002945"
     CDS             7214..7945
                     /gene="rsmE"
                     /locus_tag="OO468_002945"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR6259984.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7958..8908
                     /gene="gshB"
                     /locus_tag="OO468_002946"
     CDS             7958..8908
                     /gene="gshB"
                     /locus_tag="OO468_002946"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR6259985.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9017..9580
                     /locus_tag="OO468_002947"
     CDS             9017..9580
                     /locus_tag="OO468_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR6259986.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9580..9996
                     /gene="ruvX"
                     /locus_tag="OO468_002948"
     CDS             9580..9996
                     /gene="ruvX"
                     /locus_tag="OO468_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR6259987.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARFGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10172..11152)
                     /locus_tag="OO468_002949"
     CDS             complement(10172..11152)
                     /locus_tag="OO468_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR6259988.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11170..11874
                     /gene="yggS"
                     /locus_tag="OO468_002950"
     CDS             11170..11874
                     /gene="yggS"
                     /locus_tag="OO468_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR6259989.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11892..12458
                     /gene="yggT"
                     /locus_tag="OO468_002951"
     CDS             11892..12458
                     /gene="yggT"
                     /locus_tag="OO468_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR6259990.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12455..12745
                     /locus_tag="OO468_002952"
     CDS             12455..12745
                     /locus_tag="OO468_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR6259991.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12753..13346
                     /locus_tag="OO468_002953"
     CDS             12753..13346
                     /locus_tag="OO468_002953"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR6259992.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13339..14475
                     /gene="hemW"
                     /locus_tag="OO468_002954"
     CDS             13339..14475
                     /gene="hemW"
                     /locus_tag="OO468_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR6259993.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14789..15752
                     /locus_tag="OO468_002955"
                     /pseudo
     CDS             14789..15752
                     /locus_tag="OO468_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15797..>16294
                     /locus_tag="OO468_002956"
     CDS             15797..>16294
                     /locus_tag="OO468_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR6259994.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEA"
BASE COUNT         4021 a         4204 c         4105 g         3964 t
ORIGIN      
        1 attattcatc ttcaagataa gtataaccgt acaaacctgc ctcgaactct tcaaggaact
       61 gttgttgcag ttcagcatca agatcggttt tcttcacttg atcgcggaac tgggttaaca
      121 gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg ccttcgtcag
      181 acagttctac ttctacgcta ccgtcaggga acacgaacac gtcaactgct tcggtatcac
      241 cgaacaggtt gtgcatattg ccaaggatct cctgatatgc accgaccata aagaaaccga
      301 gcatcggcgg attctctgga tcgtactccg gcattggcat tgtcgtggca ataccatcac
      361 catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc agcacagcgc
      421 gacgttccgg cacttgatcc agcccttcca gcggtagaac cgggaacaac tggtcgatcc
      481 cccatgcgtc cggcattgac tggaacagcg agaaattgac gtacattttg tccgccatac
      541 gttcctgcag ttcgtcgatt atcggacgat gcgcacggtt ttgcggatcc agttgcttct
      601 gcacttcatg gcacatgctc agatagagtt gttctgccca tgcacgttct tgcaggctaa
      661 aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctggctg tcgtgtaacc
      721 attcacgcaa agaacggcga gttcccggtt cgtgcatttc ctgccaggtt tcccacatgc
      781 tttgcagcgc acgcggcgca tcttctacgg gcgcggtcgg caccgtatat tcgttacgtt
      841 ccacgccgat gatattcgac accagcacgg tatggtgcgc agtcaccgca cggcccgatt
      901 cggtgattac cgtcggatgc ggcagaccat tttcttcaca cgcatcgcca atcgcccaga
      961 tgatgttgtt ggcgtattca ttgaggccat agtttacgga gcagtcagac tgcgaacggg
     1021 tgccttcata atccacgccc agaccgccgc cgacgtcgaa gcactgaata ttgacgccca
     1081 gcttgtgcag ttccacatag aaacgcgcgg attcacgaac gcctgtcgcg atatcgcgaa
     1141 tattcgccat ctgcgaaccg aggtggaagt gcagtagttg caggctgtcg agacgcccgg
     1201 cttcacgcag ggtttcaacc agttgcagta cctgagtcgc agccaggccg aacttcgatt
     1261 tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt gcacgcacgc
     1321 ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg atttctgaca
     1381 tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat gccaggcgga
     1441 tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgagtcatg ccagcatgtg
     1501 ccagcactgc catcaactcg gctttggaac cggcttccag acccagcggt tcacccgaat
     1561 gaatcaggga ctcgatcacg cggcggtgct ggttaacttt gatcggataa acaaggaagt
     1621 aatcgccgtt atagccgtag gattccctcg cacgtttgaa cgcggcgtta atggaacgca
     1681 aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga ccctgtgctt
     1741 cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc gggtccgggc
     1801 acacgctgat gtggcccagc tcgttaacgt catagtagtt attgccccac caggcaatat
     1861 tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc tgcatggagc
     1921 gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg tcagacatag
     1981 cgaacctcaa attattttat taagtgtaaa acagttaacg actgtcgcag cctggaactg
     2041 cgataacaac ccataagcac atggattttc cagcagtgta tgctgacgct ccgactgcgc
     2101 aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg tgacagttcg
     2161 gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc ctcagtaact
     2221 gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc aggtatgcaa
     2281 agcagagatg cagagtgcgg ggaacgagtc ttcaccagaa cggtgagaca ggttaagcag
     2341 cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac gaacccacaa
     2401 gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg tcgtgtgctt
     2461 tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg caaaagataa
     2521 atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa cagaatgaga
     2581 cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc catccagatg
     2641 ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacctg cagagtcgtg
     2701 gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa ttctctttag
     2761 gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga agggcatcct
     2821 gacaaaattg ctgaccaaat ctctgatgcc gttttagacg cgatcctcga acaggatccg
     2881 aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt tggcggcgaa
     2941 atcaccacca gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt tcgcgaaatt
     3001 ggctatgtgc attccgacat gggctttgac gctaactcct gtgcggttct gagcgctatc
     3061 ggcaaacagt ctcctgacat caaccaaggc gttgaccgtg ccgatccgct ggaacagggc
     3121 gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt gctgatgcca
     3181 gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg taaaaacggc
     3241 actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta tgacgacggc
     3301 aaaatcgttg gtattgatgc tgtcgtgctt tccactcagc actctgaaga gatcgaccag
     3361 aaatcgctgc aagaagcggt aatggaagag atcatcaagc caattctgcc cgctgaatgg
     3421 ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gtttcgttat cggtggccca
     3481 atgggtgact gcggtctgac tggccgtaaa attatcgttg atacctacgg cggcatggcg
     3541 cgtcacggtg gcggtgcatt ctctggtaaa gatccatcaa aagtggaccg ttccgcagcc
     3601 tacgcagcac gttatgtcgc gaaaaacatc gttgctgctg gcctggccga tcgttgtgaa
     3661 attcaggttt cctacgcaat cggcgtggct gaaccgacct ccatcatggt agaaactttc
     3721 ggtactgaga aagtgccttc tgaacaactg actctgctgg tacgtgagtt cttcgacctg
     3781 cgcccatacg gactgattca gatgctggat ctgctgcacc cgatctacaa agaaaccgca
     3841 gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa agcgcagctg
     3901 ctgcgcgatg ctgccggtct gaagtaatct ttcttcacct gcgttcaaag gccagcctcg
     3961 cgctggcctt tttcttttac ctcaccgcgg tggataggcg ttcacgccgc atccggcaaa
     4021 aaaactgccc gcacaataac atcattcttc ctgatcacgt ttcacggcaa attaccatca
     4081 caactgaaac cgattacacc aaccacacca gcaaaatatt tgtaatattt tcatattatt
     4141 attcagcttt cacagttgtt acatttcttt tcagtaaagt cttaattgca gatgacagcg
     4201 tttaatctat gatgatataa ctcaattatt ttcatgccct taaatcataa ctaagataaa
     4261 tgttagtgta agcgattaca ctgatgtgat ttgcttcaca tctttttacg tcgtactcac
     4321 ctatcttaat tcacaataaa aaataactat attggagggc atcatgcctg acgctaaaaa
     4381 acaggggcgg tcaaacaagg caatgacgtt tttcgtctgc ttccttgccg ctctggcggg
     4441 attactcttt ggcctggata tcggtgtaat tgctggcgca ctgccgttta ttgcagatga
     4501 attccagata acttcgcaca cgcaagaatg ggtcgtaagc tccatgatgt tcggtgcggc
     4561 agtcggtgcg gtgggcatcg gctggctctc ctttaaactc gggcgcaaaa agagcctgat
     4621 gatcggcgca atcctgtttg ttgccggttc actgttctct gcggctgcgc caaacgttga
     4681 agtactgatt ctttcgcgcg ttctgctggg gctggcggtg ggtgtggcct cttataccgc
     4741 accactgtac ctgtcagaaa ttgcgccgga aaaaattcgc ggcagtatga tctcgatgta
     4801 tcagttgatg atcactatcg ggatcctcgg tgcttatctt tctgataccg ccttcagcta
     4861 caccggtgca tggcgctgga tgctgggtgt gattatcatc ccggcaattt tgctgctgat
     4921 tggtgtcttc ttcctgccag acagcccacg ttggtttgcc gccaaacgcc gttttgttga
     4981 tgccgaacgc gtgctgctac gcctgcgtga caccagcgcg gaagcgaaac gcgaactgga
     5041 tgaaatccgt gaaagtttgc aggttaaaca gagtggctgg gcgctgttta aagagaatag
     5101 caacttccgc cgcgcggtgt tccttggcgt actgttacag gtaatgcagc aattcaccgg
     5161 gatgaacgtc atcatgtatt acgcgccgaa aatcttcgaa ctggcgggtt ataccaacac
     5221 caccgagcaa atgtggggga cagtgattgt cggcctgacc aacgtacttg ccacctttat
     5281 cgcaatcggc cttgttgacc gctggggacg taaaccaacg ctaacgctgg gcttcctggt
     5341 gatggctgct ggcatgggcg tactcggtac aatgatgcat atcggtatcc actctccgtc
     5401 ggcgcagtat ttcgccatcg ccatgctgct gatgtttatt gtcggttttg ccatgagtgc
     5461 cggtccgctg atttgggtac tgtgctccga aattcagccg ctgaaaggcc gcgattttgg
     5521 catcacctgc tccaccgcca ccaactggat tgccaacatg atcgttggcg caacgttcct
     5581 gaccatgctc aacacgctgg gtaacgccaa caccttctgg gtgtatgcgg ctctgaacgt
     5641 actgtttatc ctgctgacat tgtggctggt accggaaacc aaacacgttt cgctggaaca
     5701 tattgaacgt aatctgatga aaggtcgtaa actgcgcgaa atcggcgctc acgattaatc
     5761 tcctcaggct tcctcccgtc gcggaggaag ccgcctcttg cagtcatctt ttcttcgctc
     5821 tatcctctgc cgctatgaaa acatcccgtc tccctatcgc catccaacag gccgttatgc
     5881 gccgcctgcg ggaaaaactc gcccaggcca acctgaagct agggcgtaac tacccggagc
     5941 caaaactctc ttacacccag cgcggaacct ccgccggaac agcctggctg gaaagctatg
     6001 aaatccgcct caatcccgtt ttgctgttgg aaaacagtga agcttttatt gaagaagtgg
     6061 taccgcacga actggcacat ttgctggtat ggaaacattt cggccgcgta gcgccacatg
     6121 gcaaagagtg gaagtggatg atggaaagcg tgctgggcgt tcccgcccgt cgtacgcatc
     6181 agttcgaact gcaatccgtg cgtcgcaaca ccttccccta ccgctgtaag tgccaggaac
     6241 atcagcttac cgtacgccgc cataatcgcg tagttcgtgg cgaggccatc tatcgctgtg
     6301 ttcactgcgg tgaacagctg gttgcgaaat aaccacctga actatcagga actttcctga
     6361 tctggctgat tgcataccaa aacaactttc gctacgttgc tggctcgttt taacacggag
     6421 taagtgatgt accgttattt gtctattgct gcggtggtac tgagcgcagc attttccggc
     6481 ccgacgttgg ccgaaggtat caatagtttt tctcaggcga aagccgcggc ggtaaaagtc
     6541 cacgctgatg cgcccggtac gttttattgc ggatgtaaaa ttaactggca gggcaaaaaa
     6601 ggcgttgttg atctgcaatc gtgcggctat caggtgcgca aaaatgaaaa ccgcgccagc
     6661 cgcgtagagt gggaacacgt cgttcccgcc tggcaattcg gtcaccagcg ccagtgctgg
     6721 caggacggtg gacgtaaaaa ctgcgctaaa gatccggtct atcgcaagat ggaaagcgat
     6781 atgcataacc tgcagccgtc agtcggcgag gtgaatggcg atcgcggcaa ttttatgtac
     6841 agccagtgga atggcggcga aggccagtac ggtcaatgcg ccatgaaggt cgatttcaaa
     6901 gaaaaagctg ccgaaccacc agcgcgtgca cgcggtgcca ttgcgcgcac ctacttctat
     6961 atgcgcgacc aatacaacct gacactctct cgccagcaaa cgcagctgtt caacgcatgg
     7021 aacaagatgt atccggttac cgactgggag tgcgagcgcg atgaacgcat cgcgaaggtg
     7081 cagggcaatc ataacccgta tgtgcaacgc gcttgccagg cgcgaaagag ctaacctaca
     7141 ctagcgggat tctttttgtt aacccctacc ccacgcgtac aaccgcgtgg ggagacgacg
     7201 cggattttta actatgcgta tcccccgcat ttatcatcct gaaccactga ccagccattc
     7261 tcacatcgcg ctttgcgaag atgccgccaa ccatatcggg cgcgtactgc gcatggggcc
     7321 ggggcaggcg ttgcaattgt ttgacggtag caaccaggtc tttgacgccg aaattaccag
     7381 cgccagcaaa aaaagcgtgg aagtgaaggt gctggaaggc cagatcgacg atcgcgaatc
     7441 tccgctgcat attcacctcg gtcaggtgat gtcgcgtggt gaaaaaatgg aatttactat
     7501 ccagaaatcg atcgaactcg gtgtaagcct cattacgcca cttttttctg agcgctgcgg
     7561 cgttaaactg gatagtgaac gtctgaacaa gaagcttcag cagtggcaga agattgcaat
     7621 tgctgcctgt gagcagtgtg gtcgtaaccg ggtgccggaa atccgtccag cgatggatct
     7681 ggaagcctgg tgtgcagagc aggatgaagg actgaaactg aatcttcacc cgcgcgccag
     7741 taacagcatc aatacgttgc ctttaccggt tgaacgcgtc cgcctgctga ttggcccgga
     7801 aggcggttta tcggcagatg aaattgccat gactgcccgc tatcaattta ctgatatcct
     7861 gttgggacct cgcgttttgc gtacagagac aactgcgctc accgccatta ccgcgctaca
     7921 agtacgattt ggcgatttgg gctaacggag aagaataatg atcaagctcg gcatcgtgat
     7981 ggaccccatc gcaaacatca acatcaagaa agattccagt ttcgctatgt tgctggaagc
     8041 acagcgtcgt ggttacgaac ttcactatat ggagatgggc gatctgtatc tgatcaatgg
     8101 cgaagcccgc gcccataccc gcacgctgaa cgtgaagcag aactacgaag agtggttttc
     8161 gttcgtcggt gaacaggatc tgccgctggc cgatctcgat gtgatcctga tgcgtaaaga
     8221 cccgccgttt gataccgagt ttatctacgc gacctatatt ctggaacgtg ccgaagagaa
     8281 agggacgctg atcgttaaca agccgcagag cctgcgcgac tgtaacgaga aactgtttac
     8341 cgcctggttc tctgacttaa cgccagaaac gctggttacg cgcaataaag cgcagctaaa
     8401 agcgttctgg gagaaacaca gcgacatcat tcttaagccg ctggacggta tgggcggcgc
     8461 gtcgattttc cgcgtgaaag aaggcgatcc aaacctcggc gtgattgccg aaaccctgac
     8521 tgagcatggc acttgctact gcatggcgca aaattacctg ccagccatta aagatggcga
     8581 caaacgcgtg ctggtggtgg atggcgagcc ggtaccgtac tgcctggcgc gtattccgca
     8641 ggggggcgaa acccgtggca atctggctgc cggtggtcgc ggtgaacctc gtccgctgac
     8701 ggaaagtgac tggaaaatcg cccgtcagat cgggccgacg ctgaaagaaa aagggctgat
     8761 ttttgttggt ctggatatca tcggcgaccg tctgactgaa attaacgtca ccagcccaac
     8821 ctgtattcgt gagattgaag cagagtttcc ggtgtcgatc accggaatgt taatggatgc
     8881 catcgaagca cgtttacagc agcagtaacc caccttagcg agaaggatct cgttgagact
     8941 ctgagtgaca gcgcccttct ttccacgcat actgggcgct gttgcttttt tgaaccagga
     9001 aacagaacct ctgacaatga atttacagca tcactttctt attgccatgc ctgctctcca
     9061 ggatccgatt ttccgtcgtt ccgtggtcta catttgcgaa cataatacca atggtgcaat
     9121 ggggatcatc gtcaacaagc cgctggaaaa tctcaaaatt gaagggattc tggaaaagct
     9181 gaagatcacg ccggagccgc gtgatgaatc aatccgtctg gataaaccgg ttatgctcgg
     9241 cggcccactg gctgaagatc gcgggtttat tttgcatact ccgccctcca attttgcttc
     9301 cagcattcgc atttcagaca acacggtgat gaccacttcc cgcgatgtgc tggaaacgct
     9361 cggcaccgat aaacaaccgt ctgacgtatt ggtggctctg ggctatgcct cctgggaaaa
     9421 aggtcaactg gaacaagaaa ttctcgataa cgcgtggcta acggccccgg cggatctgaa
     9481 tattctgttc aaaacgccga ttgccgaccg ctggcgcgag gcggcaaaac tgattggtgt
     9541 ggatattctc accatgcctg gtgtggcagg acacgcctga tgagtggaac cttactcgcc
     9601 ttcgacttcg gcaccaaaag cattggcgta gcggtcggcc aacgcattac cggcaccgct
     9661 cgccctttgc ctgcaattaa agcgcaggac ggtacgccgg actggaacct catcgaacgt
     9721 ttactgaaag agtggcagcc tgacgaaatc atcgtcggtt tgccgctgaa tatggacggc
     9781 accgaacagc cgttaaccgc cagagcgcgc aaatttgcca accgtattca tggtcgtttc
     9841 ggtgttgaag taaagctcca tgacgagcgt cttagcactg tggaagcccg tttcggtctt
     9901 tttgaacagg gcggctatcg ggcgctcaac aaaggcaaag ttgactctgc ctctgcggtt
     9961 attattctcg aaagctattt cgagcaggga tattaaggcg atctaaaagc ctggcggata
    10021 gtcgttaccg ccatcttgcc ggatgcggcg tgaacgcctt atccagcaca catctggcag
    10081 cggctgtagg tctgataaga cgcgttagcg tcgcatcaga catttattgc ctttataggc
    10141 cctataaact tgcgcatcgg gcatggcaac gtcacaaacg cccttccccc acccgctgct
    10201 gataactctg ctgaaacgtt aacatcccca ccttctgccc ggtttgaata acatgcggta
    10261 actggtgggt tttcccttcg cgaatcaaat tccccaccgc gggagtgtta atcagtaatt
    10321 caaacagcgc cacgcgtcct tcctgtttat ccacttccag cttttgtgac agcactgccc
    10381 gtaaactacc cgcaagttga ttacgtacgg gatctttttc ctgcgccgga aatgaatcca
    10441 ccagtcgctc aactgcctgc gcggcaccac gtgtatgtaa ggttgccagc accagatgcc
    10501 cggtttctgc cgccgtcagc gccagacgga ttgtctcact gtcacgtagc tctccgagca
    10561 aaatcacatc aggatcttcc cgcaatgcag cccgcaaccc cgatgcgaac gtcatacagt
    10621 gcaaaccaat ttcccgctgc tggatcaaac atcgctggct ggcatagaga tattccacag
    10681 gatcttccag cgtcagaata tgcgcatcgg catgttgatt aagatagcca accatcgccg
    10741 ccagcgtggt agatttgcca ctccccgtag cccccgtcac cagaatcagg ccattctcgc
    10801 tcttgagtaa ttccggcaat accggtggtg caccaagctg ttcgagctgt ggacagtgcg
    10861 aaggtaacaa ccgtaatgcc agagaaatac cttgccgttg cgcgaacgca ctgccacgca
    10921 accgctggtt ttccgccagc gacacggcaa aatccagctg gccattttcc agcaatattg
    10981 cccgctgatc gtcatccagc cactcccgca gtagctcttc gacgtccggc gcatcaaacg
    11041 gcgcagcttc cattctgcca cgaatgcgcc atcgtgcggg ccaggcgctg cacaggtgta
    11101 gatccgagac gttatgcttt acactaaggg ccacaatttc ttccatattc atactaagat
    11161 cctcggaaaa tgaacgatat tgcgcataac ctggcacagg tccgggacaa aatctcagcg
    11221 gctgcaacgc gttgcggccg ttctccagaa gaaattacgc tgcttgcagt cagtaaaaca
    11281 aaacctgcga gcgccatcgc agaagccatt gatgccgggc agcgtcaatt tggtgaaaac
    11341 tacgttcagg aaggggtaga taaaattcgc cactttcagg aactgggcgt aacaggatta
    11401 gaatggcatt ttattggccc gttgcagtct aataaaagcc gcctagtggc agagcatttc
    11461 gactggtgtc ataccatcga ccgtttgcgc attgctaccc gcctcaacga gcagcgcccg
    11521 gcagaacttc cccctcttaa cgtactgatt caaattaata ttagtgatga aaacagtaag
    11581 tccgggattc aactggcaga actggacgag ctggcagcgg cggtcgctga actaccgcgt
    11641 ttacgtctgc gcgggctgat ggcaatccct gcgcttgagt cagaatatgt aaggcagttt
    11701 gaagttgcac gccaaatggc tgtagcattt gccggactga aaacgcgcta cccgcatatc
    11761 gacacgctct ctctgggaat gtcggacgat atggaagccg ccattgcggc aggtagcacg
    11821 atggttcgta tcggcactgc aatttttggt gcgcgtgatt actctaaaaa ataaggaatt
    11881 aaaggaacgc catgaatacg ttgactttcc tgctttcaac ggtcattgag ctgtatacaa
    11941 tggtgctgtt attacgcatc tggatgcagt gtgctcattg tgatttttac acccccttct
    12001 cacagtttgt agtgaaggta gcgcagccaa ttatcgggcc actgcgccgc gttattccgg
    12061 caatgggtcc cattgacagc gcctcgctgc tggttgccta tattctcagt tttatcaaag
    12121 ccattgtgct gtttgaagtg gtgaccttcc tgccaatcat ctggattgcc ggtttactga
    12181 ttctgctgaa aaccatcggc ttgctgattt tctgggtcct gctggtgatg gcgattatga
    12241 gctgggtaag ccagggtcgt agcccgattg aatacgtgct gattcagctg gccgatccgc
    12301 tgctgcgccc gattcgccgc ttgctaccgg caatgggtgg gattgatttc tcgccgatga
    12361 tcctcgttct gctgctgtat gccatcaata tgggtgtcgc agaagtatta caggcaaccg
    12421 gaaacatgct gctgccgggg ctgtggatgg cgttatgagt gccgtaacag ttaatgatga
    12481 cggtctggtt ttacggctct atattcagcc gaaagccagc cgtgattcta ttgtcggttt
    12541 acatggcgac gaagttaaag tcgccattac cgcgccgccg gttgacggcc aggccaacag
    12601 tcatctggtg aagtttctcg gtaagcaatt ccgggttgcc aaaagccagg tggtgattga
    12661 aaaaggcgaa cttggccgcc acaaacaaat taaaatcatt aatccgcaac aaatcccgcc
    12721 agaaatcgcg gcgttaatta attaggtatc ctatgcaaaa agttgtcctc gcaaccggca
    12781 atgccggtaa agtgcgtgag ctggcgtcgc tgcttagcga ctttggtctt gatatcgtgg
    12841 cccaaacaga cctcggcgtt gattccgctg aagaaaccgg cctaaccttt atcgaaaacg
    12901 cgattctgaa agcgcgccat gcggcaaaag tgaccggttt accggcaatt gccgacgact
    12961 ctggtctggc ggtagatgcg ctgggtggcg cgccgggtat ttactccgcg cgttattccg
    13021 gtgaagacgc gaccgatcaa aagaatctgc aaaaactgtt ggaaacactg aaagacgtac
    13081 cggacgacca acgtcaggcg cgtttccact gcgtgctggt atatctgcgt cacgcggaag
    13141 atccgactcc gctggtatgc cacggcagct ggccgggcgt gattactcgc gaaccagcgg
    13201 gcactggtgg ctttggttat gatccaatct tcttcgtacc ttccgaaggg aaaaccgccg
    13261 ccgaactgac ccgcgaagaa aagagcgcca tttcccaccg tggtcaggcg ttgaaactgc
    13321 tgctggacgc tttacgtaat ggttaaatta ccgccgctga gtctctacat tcacatcccg
    13381 tggtgcgtgc agaaatgccc gtactgcgat ttcaactctc acgcgttgaa aggagaagtg
    13441 ccgcacgacg attatgttca gcatctgctt tgcgatctgg acaatgatgt ggcttacgct
    13501 cagggccgtg aagtaaagac aatctttatt ggcggtggta cgccgagcct gctttccggc
    13561 ccggcgatgc aaacgctgct agacggcgtg cgtgcgcgtt tgccgctggc agcggatgca
    13621 gaaattacta tggaagcgaa cccaggcacg gtagaagcgg atcgctttgt cgattatcag
    13681 cgtgctggtg taaaccgcat ctctattggt gtgcagagtt ttagcgaaga aaagctgaaa
    13741 cgacttgggc gtattcatgg cccgcaagaa gcgaaacgag cggcaaagct ggcgagcggt
    13801 ttagggttac gtagctttaa ccttgatttg atgcatggac taccggatca atcactggaa
    13861 gaggcgcttg gcgatctacg ccaggccatt gaactgaatc cgccgcatct ttcctggtat
    13921 caactgacta tcgaacctaa tacgctgttt ggctcgcgac cgccggtgct gccggacgat
    13981 gacgcgctgt gggatatttt cgaacagggg catcagttat taaccgcagc gggttatcag
    14041 caatatgaaa cttccgctta cgccaaaccc ggttatcagt gccagcacaa tctcaactac
    14101 tggcgctttg gtgactacat cggtattggc tgcggcgcgc acggcaaagt gaccttcccg
    14161 gatgggcgca ttctgcgtac cactaaaacg cgtcatcctc gtggttttat gcaaggaagg
    14221 tatctggaaa gccagcgtga tgtcgaagcc gcagataagc cgtttgagtt ctttatgaat
    14281 cgcttccgtc tgctggaggc cgcgccgcgc gtggagttta ttgcgtatac cgggctttgc
    14341 gaagatgtga ttcgcccaca gttagacgag gcgattgccc agggttatct caccgaatgt
    14401 gcggattact ggcagataac ggaacatggt aaattattct taaactcact tcttgaatta
    14461 ttcctcgctg agtaatcatt aatgcgcctg cgggcgcatt aatcacttct tttatccccc
    14521 cgttaattaa cctcggcaga aatatatttc ttactgaata aatatcgacg acatttatta
    14581 tatatattta gtgaatattt actaatcatt cattgattat aaatatgtac aacctggcgc
    14641 acaaatttaa tatttaattt ctgactttat ttcaagttgt gataaacata gacaagttgt
    14701 cagacaggtt aaccaatttg tcgcacaaat cactgataag cacaattaga tatcaaccaa
    14761 tttccatcct tcaacaggga gtttttttat gaaaatgagg tcatttaccc gctcattagt
    14821 ctgcgcatcc ttgctggctc tggttagcac tggcgtaaac gccgcagaaa aagtggcgtt
    14881 aaagttagca tacaacctgg aaagaagtca tgttgtacat cagagttttg aagagctggc
    14941 gaaagaggta aaacagctgt caaaaggtaa tatggtgatc cgtatttatc ccagcagcca
    15001 gatgggtaat gcgcgagaaa ctatggaatt gttgcaaaat ggcgcactgg atatgaccaa
    15061 aggttccgcc agtgatctgg agtcttttga taatatttat gctatctata atttgccgtt
    15121 cttattaagt tgtttttggt gaagttggta aagaaattat ggactcaaca aaagacaaag
    15181 gcttttttgc tttatcagct tatgtagcgg gtactcgcag tttctatgcg aaaaaaccga
    15241 ttacaaagcc agaagatctt aaaggactga aaattcgtgt tcagccaagt ccgaccacca
    15301 tcaaaatgat tgaactgatg ggtggttcac ccactccaat ttcatttggc gaagtttata
    15361 ctgcgatgca gcaaggtgta gtagatggcg cagaaaataa cgtgccttcc tggatgcaga
    15421 ctcgacatat tgaaattgcc aaagtttttt ctgaagatga acacgcctca attcctgact
    15481 ttttggttat ctccaccaaa acatggaata aattaacccc agaacagcaa caaatcctcg
    15541 aaacagcggc gaagaaatcc gaagcttatc aacaaaaatt gtgggaaaaa attgacgctg
    15601 acacacgtgc tcaggcgaaa gctatggggg gtgaaattgt aaaagtggat aaagcgccat
    15661 tccgtaaagc cgtgcaacct ctgtttgacg acttcaaaaa agatccaaaa caagctgctt
    15721 tgttagagaa attcgacaac gctgctcaat aatattccgg gccagcctct ggccctgatt
    15781 ttccaggatg cttacaatga tatttaaccg cctgaaactt gcggtagacc gcgtcattgc
    15841 cacgttttct gtcgccgtaa tgcttgctct ggttgtatgc gtagtctggc aagtatttag
    15901 ccgctacgta cttaaccagc caagtacatt aaccgatgag ctggcacgct ttttgatgat
    15961 ctgggtagga ttattaggtg ctgcatatac cgtcggcgcg cagcgacatc tctctattga
    16021 tttatttgct ctcgccctga ataagcgcaa acaattactt cttagtatag tcattaacgt
    16081 tctcatttta ggttttgccg gttcggtcat tgtgactggt ggcctgaaat taattgataa
    16141 aacactggca acatcacagg tatctgccgc tatgcaaata ccgatgggat acgtatatat
    16201 cattctgcca ttaagtggag tggtaatgat gttttatgcc ttgtgtttta ttaatcaaag
    16261 cattcaacaa ctgaaacaac ctgcgcagga ggcc
//
LOCUS       DAJTUP010000088        15636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTUP010000088 DAJTUP010000000
VERSION     DAJTUP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.088"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            336..629
                     /locus_tag="OO468_002957"
     CDS             336..629
                     /locus_tag="OO468_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR6259995.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            701..1360
                     /locus_tag="OO468_002958"
     CDS             701..1360
                     /locus_tag="OO468_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR6259996.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1452..1898
                     /locus_tag="OO468_002959"
     CDS             1452..1898
                     /locus_tag="OO468_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR6259997.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2105..3005)
                     /gene="hlyE"
                     /locus_tag="OO468_002960"
                     /pseudo
     CDS             complement(2105..3005)
                     /gene="hlyE"
                     /locus_tag="OO468_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3378..3797
                     /locus_tag="OO468_002961"
     CDS             3378..3797
                     /locus_tag="OO468_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR6259998.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3797..5065
                     /gene="umuC"
                     /locus_tag="OO468_002962"
     CDS             3797..5065
                     /gene="umuC"
                     /locus_tag="OO468_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR6259999.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5111..5641)
                     /gene="dsbB"
                     /locus_tag="OO468_002963"
     CDS             complement(5111..5641)
                     /gene="dsbB"
                     /locus_tag="OO468_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR6260000.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5787..7328)
                     /gene="nhaB"
                     /locus_tag="OO468_002964"
     CDS             complement(5787..7328)
                     /gene="nhaB"
                     /locus_tag="OO468_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR6260001.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7549..8268
                     /gene="fadR"
                     /locus_tag="OO468_002965"
     CDS             7549..8268
                     /gene="fadR"
                     /locus_tag="OO468_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR6260002.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8320..9852)
                     /locus_tag="OO468_002966"
     CDS             complement(8320..9852)
                     /locus_tag="OO468_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR6260003.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10182..11480
                     /gene="dadA"
                     /locus_tag="OO468_002967"
     CDS             10182..11480
                     /gene="dadA"
                     /locus_tag="OO468_002967"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR6260004.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11490..12560
                     /gene="dadX"
                     /locus_tag="OO468_002968"
     CDS             11490..12560
                     /gene="dadX"
                     /locus_tag="OO468_002968"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR6260005.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12728..14142)
                     /locus_tag="OO468_002969"
                     /pseudo
     CDS             complement(12728..14142)
                     /locus_tag="OO468_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14753..>15636)
                     /locus_tag="OO468_002970"
     CDS             complement(14753..>15636)
                     /locus_tag="OO468_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6260006.1"
                     /translation="LGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFN
                     LRERVFISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAA
                     KKAKVVVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAA
                     LFRDNQLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEA
                     SAKYADVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKED
                     NEVLKIGVRVAEEEAES"
BASE COUNT         3939 a         3762 c         3999 g         3936 t
ORIGIN      
        1 tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc agtgcaagca
       61 gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc agaatgagta
      121 cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct tttctgcgtt
      181 cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg ggcatggact
      241 caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat tgatgttagc
      301 ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgcgatt tatcgaagca
      361 gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg
      421 aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg ttggatgggc
      481 ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc gagcaaggtt
      541 actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt tccgtcatgg
      601 ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt atctttgtcg
      661 gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac atcacaactg
      721 gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggca gtaactatgc
      781 caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc
      841 agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt tcggttcagt
      901 tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg ctgcgtcagg caacggaaga
      961 gcatgtccgc aaagccattg ccggttacgg cgtggcgctt gatctgacat tgcgtgatgt
     1021 tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt ttgataactc
     1081 ttgtccgctt tccgggttta tccccgcggc ggaatttacc ggcgatccac aaaatacaac
     1141 gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt acgactgcgg acatgatcca
     1201 taaaatcgtt ccgttgatcg cttatatgag caagtttttt accctcaagg ccggtgacgt
     1261 tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg agctgacagt
     1321 cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc cgccagaaag
     1381 cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg
     1441 cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac gaaatgagcg
     1501 atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcttgcat aaactgatgg
     1561 atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc aatattaaaa
     1621 cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc attaaattaa
     1681 cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat cggttgctgg
     1741 cggaaggtaa agatttacct gcgtggcatc cgctacttac tggttcgaaa gcggcaatgc
     1801 atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg attgactggc
     1861 aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag atgctgatgg
     1921 gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt tgaaagcgct
     1981 ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca atctttaaaa
     2041 aacgtctgtg tttttaaatg tgataaacct tataccttga gtacatggag agaataatcg
     2101 cttatcagac ttcaggtact tcaaagagtg tctttttacc gtgtcttttc tgatactcat
     2161 tacaggtgtt aatcattttt ttggccgctg cttttagcaa agaaagcatt aaatcatcat
     2221 aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg gcggctattt
     2281 cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta accgtgttag
     2341 acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct ggaatcagtt
     2401 tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat ggaccggcga
     2461 cgacatcggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc tgtgactgga
     2521 aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc agcagtttcc
     2581 cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct tcattcagct
     2641 tcgtgatgcc gtcatccagt accttaatga ggggcggatg ctttcttctc attgtactca
     2701 tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca ttcatacact
     2761 gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg
     2821 actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt
     2881 tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct
     2941 ccatctgcgg tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca
     3001 gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta
     3061 ttaataaatt ttacttcatc taattcatag ttagccgggc gggatgcgtc aatgtcttta
     3121 tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat
     3181 tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg
     3241 ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga
     3301 tagcctgaat cagtattgat ctgctggcaa gaacagacca ctgtatataa aaacagtata
     3361 acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt
     3421 ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgttg
     3481 aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca
     3541 aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg
     3601 atagtgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta
     3661 cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact
     3721 cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg
     3781 ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg ccagctgtga
     3841 gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg
     3901 ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg gcgatccctg
     3961 gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga
     4021 gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt
     4081 cgagatttac agtattgatg aagcattctg cgatctgaca ggtgtgcgta attgtcgcga
     4141 tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt
     4201 tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt gccaatcatg cggcaaaaaa
     4261 atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc agcgtaaatt
     4321 aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca gcaaaaaact
     4381 ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc ggtttatccg
     4441 taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa ctgcgcggcg aaccctgttt
     4501 gcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct cgtttggtga
     4561 acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg cccgggcggc
     4621 ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta ttaagacgtc
     4681 accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac tgctgacgcc
     4741 cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg ccatctggca
     4801 agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca gtcagggagt
     4861 cgcgcagctc aatttattcg atgacaacgc accgcgcccc gggagtgagc aattgatggc
     4921 ggtaatggat actctgaatg ctaaagaggg cagaggaaca ctctattttg ccgggcaggg
     4981 gatccaacaa caatggcaga tgaagcgagc tatgctttca ccacgttata caacgcgaag
     5041 ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgctc ccgcaggagc
     5101 gccgaatgga ttagcgaccg aacagatcac gttttttcgc tttaaacggc tgggaaatca
     5161 ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac tgcggcattt
     5221 ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca aacacttgcg
     5281 gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca caggtggcaa
     5341 atggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca ccgcggaacg
     5401 cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg gcgatcgcgc
     5461 caatcagcgc agcacccaga acgccgaata acgcgcagcg ttcataaata cagagcacgc
     5521 aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt gccagagcag
     5581 taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa aatcgcaaca
     5641 taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat tcgctgcgcc
     5701 accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa ggcaaaccgg
     5761 gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat ttgcataaac
     5821 cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag tgtcagtacg
     5881 agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat caatggcgcg
     5941 agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc gacagagggc
     6001 agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa cgtgatagcg
     6061 ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa gacgttatcc
     6121 gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc atgttcagat
     6181 gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat caccgcgact
     6241 accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa ggctttaccg
     6301 atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac tgacaaacca
     6361 atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac gccaataatc
     6421 gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct ttgatcgtca
     6481 aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc aaaccaacgc
     6541 agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac ggtcaccggc
     6601 gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat gatcaggttc
     6661 tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc ggcatgcatc
     6721 atcaggctac gcagaaagcc acggaactgt tccagaacca cgttgtaatg cttgtcgata
     6781 tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg atgataaata
     6841 ccataaaaac cgactgcaac gctgatcacc accgccacga cggttaaggc atcgaggaac
     6901 gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt ggagcgaatg
     6961 cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata gatacccgcc
     7021 accataaaca tcaataacag caagacttca agatttgccg ccacctcttc acggacgtgt
     7081 tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc ggggagtagc
     7141 gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag caaccagccc
     7201 gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat gatgagggcg
     7261 agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg gccccaggag
     7321 atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat gtttaaacgc
     7381 aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga aagagatgcg
     7441 gaaaatgaag ccttgatccc tttttcttct ttttgtctgc tatcagcgta gttagccctc
     7501 tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat ggtcattaag
     7561 gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg gaataaccgc
     7621 ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg
     7681 cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt gaccattcaa
     7741 catggcaagc cgacgaaggt gaataatttc tgggaaactt ccggtttaaa tatccttgaa
     7801 acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt gctgtcggtg
     7861 cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc cgataaagcg
     7921 caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt tgccgagctg
     7981 gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta cggtctgatc
     8041 cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc caatccggaa
     8101 gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag tgaaggcgcg
     8161 cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga gatttggcac
     8221 cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc ccttccgttt
     8281 aaagagcaac cccctcaaac gaggggtttt ttgttgtttt tacagatttc ccattcttgg
     8341 cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga gcatcacatc
     8401 aaatccccac aggcgatgca catgcttcag gacttctttg cgcccccgat ccagcggtgc
     8461 gcgattatgt ggaatataac gcagcgtcag cgaacggtcg ccgcgcaaat ccacgttcca
     8521 gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc ggttacggat
     8581 ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat gccgatcatc
     8641 atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa actggctgat
     8701 aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc agtcggaacc
     8761 ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa tccgtttaat
     8821 atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc acgggctgtt
     8881 ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca taaaacgttc
     8941 cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga aggtcgcaca
     9001 gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca ccttacgcac
     9061 aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct ccataaaata
     9121 gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg ttttctcttc
     9181 ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat actcttcgcg
     9241 acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt tgtaccggtc
     9301 cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt catcaacgcc
     9361 ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat cgacaatcga
     9421 actggcgtcg gtccaactgc ggaataagca attgttttta aagaaagagt tatgtccata
     9481 gcaggcgtgt gccatcacca gcgcctgcat agtaatggtg ttctcttcca tcaagtaagc
     9541 gatacacggg ttagagttaa tgacgatttc ataggccagc ccttgctgac cgtgcttata
     9601 cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt taattggcat
     9661 gccgacgctg gaataggcat ccatcatctg ttctgaggtt atcacttcaa tctggtgcgg
     9721 gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat aaacatccag
     9781 caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat tcatagaatc
     9841 gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag cacctcattt
     9901 caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt tgcgatttct
     9961 tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat aaaaagggcg
    10021 ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg tgagtcagct
    10081 caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag atgtaattag
    10141 attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt gtcatactgg
    10201 gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga catgaggtca
    10261 ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat gccgggcaaa
    10321 tctcccccgg atatgctgca ccgtgggcag caccaggtgt gcctttaaaa gcgattaaat
    10381 ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag tttcagttga
    10441 aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa aacaaagggc
    10501 ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc gccgaaacca
    10561 atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc gaacaacagt
    10621 atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg tatcagctgc
    10681 tggaatccag ccgcctggcg gaagtggatc ccgcgctggc agaagtagcg cacaaactga
    10741 cgggcggcct gcagttaccc aatgatgaaa ccggagactg ccagctgttt acccagaatc
    10801 tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc gttgaccaac
    10861 tgctttgcga cggcgagcaa atctacggcg tgaagtttgg cgatgaagtg attaaggccg
    10921 atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc attgttgata
    10981 ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag gaagatggtg
    11041 cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt ttcgataacc
    11101 gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg ttgcaaccgc
    11161 gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt catgtcgagc
    11221 aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg gttgtcggga
    11281 gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc tggacgatgg
    11341 cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca gcgatcccat
    11401 atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt ccgggccatt
    11461 tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc agcctcgatc
    11521 tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac gcgcgcgtct
    11581 ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg agcgcgctcg
    11641 gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta cgtgagcgcg
    11701 gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat ctggagattt
    11761 atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa gcactgcaaa
    11821 atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg atgaatcggt
    11881 tgggcttcca gtccgatcgc gtgcttaccg tctggcagca gttgcgggca atggcgaatg
    11941 ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga acatcctgat ggaatttccg
    12001 gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt tcgttgtcca
    12061 attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg cctggcatta
    12121 ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc ggattacgtc
    12181 cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg ggcgagcgtg
    12241 tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt gtcgccgcag
    12301 gatacgccga cggttatccg cgccatgcgc ctaccggtgc tcctgtttta gtggacggcg
    12361 tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat ttaacgcctt
    12421 gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc aaaattgatg
    12481 atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcactg gcgttacgcg
    12541 tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg aggcagcgtc ttctggttgc
    12601 gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca gagccattgc
    12661 ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa ctcgccgcac
    12721 aggcgttcta cataaaacgc ttacgcttca ttgttgactc ggagtcgact tcagataaat
    12781 cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta acaggctgcg
    12841 agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc tccaggtcct
    12901 cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga tccataaggt
    12961 cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa aagtccaaaa
    13021 ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc gtcggtaaat
    13081 tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg ggctgcggca
    13141 cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg ggcggcagcc
    13201 gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga gtcaagttct
    13261 ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt ttatacaggt
    13321 actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta atcactctca
    13381 ggttagtcga cagctcaggc agccagataa tactgttttc atagcacttc agtaccatca
    13441 gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag tcaagcacca
    13501 gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg cagtaaaatt
    13561 caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg ctaagattga
    13621 gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct gcccggtttt
    13681 caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct tccagacttt
    13741 ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct tccaggattt
    13801 cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc cattcgcacc
    13861 atttggcgac catcatgaac tcgttatgtt gctgattcag acgagtcccc aacgtgatga
    13921 tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca atgatctgcc
    13981 agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc tgcaaaattt
    14041 gattcttgag aagagccgcc ctggcgggcg tcgtacgcct gggttaacgg aagatggggg
    14101 aatggcgggg gcgaatgggc agatgtcttt aaagctagcc ataatgctac gtttccttga
    14161 aataaatatc ctctggcagc gccggagacg tgaatgtggt caccgcaaag gagccacagg
    14221 acgtcatttg cgctgatatc actactcatc gttgactttt ttcctggtca gaaatgccgt
    14281 gtcgggtctg gtacatgctg tattacggcc taggtagctc tgacgtatta aagaaaatac
    14341 gagagtgaac gacccggaaa taatcgttat taagcaaagg aataactata agaaatcgct
    14401 catgcatccg gtgctacagg cggatgaaac gcctattgtg tgggctatgt cagtgcggca
    14461 ggttgcgtgg gggaatatgt tcaggcgatg tcaggcaggg gcccctgatg gttgaccggt
    14521 aatgcggggc tggtttttat tacctgttac agtcggaaaa agcccttacg ctgactttct
    14581 tgagccggtg ttttgagttc attagccaga gcctgcaatg gtttgtcatc cataatttaa
    14641 ccttcttctg atgttgggat gagcatatca aaatcaggaa atgacaagtt tattacaggc
    14701 tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat gctgcattaa atttaagatt
    14761 cagcttcctc ttcagccacc cgaacgccaa tcttcaacac ttcattatct tctttctcgg
    14821 ccaccgtcca gatcatcccg gcaaactcta cctggtcacc gacaaccggt gccgcgccta
    14881 gcaactgctg gacaatttca cccagcgttt gctgtttatc acgatattct cgcccgtctt
    14941 ccaggccata tatcagcgca acatcagcat atttggcgct ggcttcgaga atgaagtcac
    15001 caaagaagcg ttgatccagc gcgaccggtg gcgactggct gaacagttta ccgagcgccg
    15061 ggagatcgcg ttcccgacca attacacaca atacatcgcc ttcgcgcagt cgggtgctgc
    15121 cggtaggatg aagcaactgg ttatcacgaa acagggctgc aatacgcgtc tcttttggca
    15181 tatgcaaatc gcgcagtgcc gcacctacac accatttatc agcactcaat tgataaacaa
    15241 actgctccca cggattttcc ggatgaatat ccaggccaac gcgtgacacc ggacgtccga
    15301 cgggcggaac caccactttg gcttttttcg ccgcccacga gagtgatgtt ccctgcaaga
    15361 gcagtgaaac cagaaccaca aagaaggcga cattaaagaa cagtcgcgca ttctccagcc
    15421 ccgccatcat cgggaacacg gctaggatga ttggcaccgc gccgcgtaat cccacccagc
    15481 tgataaacac gcgctcacgc agattgaaac cgcggaaagg gagcaatcca gcaaataccg
    15541 aaaggggacg ggcgaagaat atcatccatg ccgacaaaat gagcgccgga atggcaatag
    15601 gcagcagatc gcttgggtta accaatagcc ccagca
//
LOCUS       DAJTUP010000089        15486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTUP010000089 DAJTUP010000000
VERSION     DAJTUP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.089"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO468_002971"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO468_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR6260007.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO468_002972"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO468_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR6260008.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO468_002973"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO468_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO468_002974"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO468_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR6260009.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO468_002975"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO468_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR6260010.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO468_002976"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO468_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR6260011.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO468_002977"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO468_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR6260012.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO468_002978"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO468_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR6260013.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO468_002979"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO468_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR6260014.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO468_002980"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO468_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR6260015.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO468_002981"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO468_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR6260016.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO468_002982"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO468_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR6260017.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO468_002983"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO468_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR6260018.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO468_002984"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO468_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR6260019.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO468_002985"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO468_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR6260020.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO468_002986"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO468_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR6260021.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO468_002987"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO468_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR6260022.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO468_002988"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO468_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR6260023.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO468_002989"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO468_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR6260024.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO468_002990"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO468_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR6260025.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO468_002991"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO468_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR6260026.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO468_002992"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO468_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR6260027.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO468_002993"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO468_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR6260028.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO468_002994"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO468_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR6260029.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO468_002995"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO468_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR6260030.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO468_002996"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO468_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR6260031.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO468_002997"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO468_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR6260032.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO468_002998"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO468_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR6260033.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO468_002999"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO468_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR6260034.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO468_003000"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO468_003000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR6260035.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="OO468_003001"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="OO468_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR6260036.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         3998 a         3631 c         4126 g         3731 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca atgtaaaaaa acccgccccg gcgggttttt
    15481 ttatac
//
LOCUS       DAJTUP010000090        15403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTUP010000090 DAJTUP010000000
VERSION     DAJTUP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.090"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1006
                     /gene="citC"
                     /locus_tag="OO468_003002"
     CDS             <1..1006
                     /gene="citC"
                     /locus_tag="OO468_003002"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR6260037.1"
                     /translation="AEIAQFLHENDLSVDTTVEVFITVTRDEKLIACGGIAGNIIKCV
                     AISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTCVPGVM
                     VLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLF
                     LVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCY
                     TEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAAPIELVEI
                     ERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA
                     "
     gene            1021..1317
                     /gene="citD"
                     /locus_tag="OO468_003003"
     CDS             1021..1317
                     /gene="citD"
                     /locus_tag="OO468_003003"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR6260038.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1314..2222
                     /gene="citE"
                     /locus_tag="OO468_003004"
     CDS             1314..2222
                     /gene="citE"
                     /locus_tag="OO468_003004"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR6260039.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2233..3765
                     /gene="citF"
                     /locus_tag="OO468_003005"
     CDS             2233..3765
                     /gene="citF"
                     /locus_tag="OO468_003005"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR6260040.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3769..4319
                     /gene="citX"
                     /locus_tag="OO468_003006"
                     /pseudo
     CDS             3769..4319
                     /gene="citX"
                     /locus_tag="OO468_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4294..5172
                     /gene="citG"
                     /locus_tag="OO468_003007"
     CDS             4294..5172
                     /gene="citG"
                     /locus_tag="OO468_003007"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR6260041.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5223..6685
                     /gene="citT"
                     /locus_tag="OO468_003008"
                     /pseudo
     CDS             5223..6685
                     /gene="citT"
                     /locus_tag="OO468_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            6799..7605
                     /gene="rna"
                     /locus_tag="OO468_003009"
     CDS             6799..7605
                     /gene="rna"
                     /locus_tag="OO468_003009"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR6260042.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            7834..8244
                     /gene="rnk"
                     /locus_tag="OO468_003010"
     CDS             7834..8244
                     /gene="rnk"
                     /locus_tag="OO468_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR6260043.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8475..9684)
                     /locus_tag="OO468_003011"
                     /pseudo
     CDS             complement(8475..9684)
                     /locus_tag="OO468_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
     gene            9905..10333
                     /gene="uspG"
                     /locus_tag="OO468_003012"
     CDS             9905..10333
                     /gene="uspG"
                     /locus_tag="OO468_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR6260044.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10454..12019)
                     /gene="ahpF"
                     /locus_tag="OO468_003013"
     CDS             complement(10454..12019)
                     /gene="ahpF"
                     /locus_tag="OO468_003013"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR6260045.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12264..12827)
                     /gene="ahpC"
                     /locus_tag="OO468_003014"
     CDS             complement(12264..12827)
                     /gene="ahpC"
                     /locus_tag="OO468_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR6260046.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13199..13945
                     /gene="dsbG"
                     /locus_tag="OO468_003015"
     CDS             13199..13945
                     /gene="dsbG"
                     /locus_tag="OO468_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR6260047.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14155..15057
                     /locus_tag="OO468_003016"
     CDS             14155..15057
                     /locus_tag="OO468_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6260048.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15204..15403
                     /locus_tag="OO468_003017"
                     /pseudo
     CDS             15204..15403
                     /locus_tag="OO468_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3760 a         4013 c         3776 g         3854 t
ORIGIN      
        1 ggcggaaatc gcccaattcc tgcatgaaaa tgatttgagc gttgacacca cagtcgaagt
       61 atttattacc gtaacccgcg atgaaaagct tatcgcgtgc ggtggaattg ccggaaatat
      121 tattaaatgc gttgctatca gtgaatccgt tcgcggtgaa ggactggcgc tgacattagc
      181 cactgaactg ataaacctcg cctatgagcg gcacagcacg catctgttta tttataccaa
      241 aaccgaatac gaggcgctgt tccgccagtg cggtttttcc acgctgacct gcgtacccgg
      301 cgtgatggtg ctgatggaaa acagcgccac gcgactgaaa cgctatgccg aatcgctgaa
      361 aaaatttcgt catccaggga acaagattgg ctgcattgtg atgaacgcca atccctttac
      421 gaatggtcac cgttatctga ttcaacaagc tgcggcacag tgcgactggt tgcatctgtt
      481 tttagtcaaa gaagattctt cacgcttccc ctatgaagac cggctggatc tggtgttaaa
      541 aggcaccgcc gatattccac gcctgactgt gcatcgcggc tcagaataca tcatctcccg
      601 cgctacgttc ccttgctact tcattaaaga acagagcgtc attaaccatt gttacaccga
      661 aattgatctg aagattttcc gtcagtacct cgctcccgcg cttggtgtaa ctcaccgctt
      721 tgtcggtact gaaccctttt gtcgcgttac cgcccagtac aaccaggata tgcgctactg
      781 gctggaaacg ccgactatct ccgcagcgcc catcgaactg gttgaaattg agcggctgcg
      841 ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa ctgctggcga aaaacgatct
      901 cacggctatc gcgccgctgg tccctgcagt cacgctgcat tatttgcaga acctgcttga
      961 gcactcccgc caggacgcgg cagctcgtca aaagaccccc gcatgagaaa caggtgaaaa
     1021 atgaaaataa accagcccgc cgttgcaggc acccttgagt ctggggatgt gatgatacgc
     1081 atcgccccac tcgatacgca ggatatcgac ctgcaaatca atagcagcgt tgagaaacag
     1141 tttggcgatg caattcgcac caccattctg gacgttctcg cccgctacaa cgtgcgcggc
     1201 gtacagctga atgtcgatga caaaggcgca ctggactgca ttttacatgc acgactggaa
     1261 gccctgctgg cacgcgccag cggtatcccg gctctgccat gggaggattg ccaatgattt
     1321 ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag catgttgttt gtgcctggtg
     1381 ccaatgccgc gatggtcagc aactccttca tctacccggc tgatgccctg atgtttgacc
     1441 tcgaagactc cgtggcatta cgtgaaaaag acaccgcccg ccgcatggtt tatcacgcgc
     1501 tgcaacatcc gctgtatcgc gatattgaaa ccattgtgcg tgtcaacgcg ctggattccg
     1561 aatggggtgt taacgacctg gaagccgtcg ttcgcggtgg tgcggacgtt gtgcgtctgc
     1621 cgaaaaccga taccgctcag gatgttctgg atattgaaaa agagatcctg cgtatagaaa
     1681 aagcctgtgg tcgtgaaccc ggcagcaccg gcctgctggc ggcgattgaa tctccgctgg
     1741 gcattacccg cgcagtggaa atcgctcacg cttccgagcg tttgatcggt atcgccctcg
     1801 gtgcagaaga ctatgtgcgc aacctgcgta cagaacgctc cccggaagga actgaactgc
     1861 tgttcgcacg ctgttccatt ttgcaggccg cgcgctctgc gggtattcag gcgttcgata
     1921 ccgtctattc cgacgctaac aacgaagccg gattcctgca agaagccgcc cacatcaaac
     1981 agctgggctt tgacggcaaa tcgctgatca acccgcgtca gattgatctg ctgcacaacc
     2041 tctacgcacc gacccagaaa gaagtggatc acgcccaccg cgtcgtagaa gccgctgaag
     2101 ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg caagatggtg gacggtccgg
     2161 ttatcgatcg cgcccgtctg gtgctctccc gtgcagaact ttccggcatc cgcgaagaat
     2221 aaggcaatca aaatgacgca gaaaattgaa caatctcaac gacaagaacg ggtagcggcc
     2281 tggaatcgtc gcactgaatg cgatcttgcc gctttccaga actcaccaaa gcaaacctac
     2341 caggctgaaa aagcgcgcga tcgcaaactg tgcgccaacc tggaagaagc gattcgtcgc
     2401 tcaggtttac aggacggcat gacggtttcc ttccatcacg ctttccgtgg cggtgacctg
     2461 accgtcaata tggtgatgga cgtcatcgcg aagatgggct ttaaaaacct gaccctggcg
     2521 tccagctccc tgagtgattg ccatgcgccg ctggtagaac acattcgcca gggcgtggtt
     2581 acccgcattt atacctctgg cctacgcggt ccactggcgg aagagatctc ccgtggtctg
     2641 ctggcggaac cggtgcagat ccactctcac ggcggtcgtg tgcatctggt acagagcggc
     2701 gaactgaata tcgacgtggc tttcctcggc gtcccgtcct gtgatgaatt cggtaatgcc
     2761 aacggctaca ccggtaaagc ctgctgcggc tccctcggct atgcaatggt tgatgccgac
     2821 aacgcaaaac aggtcgtgat gcttaccgaa gaactgctgc cttatccgca taatccggca
     2881 agcattgagc aagatcaggt tgatttgatc gtcaaagtta atcgcgttgg cgatgctgca
     2941 aaaatcggcg ctggtactac ccgtatgacc actaacccgc gcgaactgct tattgcccgt
     3001 agcgctgcgg atgtgattgt caactctggc tacttcaaag aaggtttctc catgcaaacc
     3061 ggcaccggcg gcgcttcgct ggcggtaacc cgtttcctgg aagacaaaat gcgtagccgc
     3121 gatattcgcg ccgacttcgc cctcggcggt attaccgcga cgatggttga tctgcacgaa
     3181 aaaggtctga tccgcaaact gctggatgtg cagagctttg acagccatgc tgcgcaatcg
     3241 ctggcccgta accccaatca catcgaaatc agcgccaacc agtacgctaa ctggggttcg
     3301 aaaggcgcat cggttgatcg tctcgacgtg gtggtactga gcgcgctgga aattgacagg
     3361 cagttcaacg ttaacgtgct gaccggttct gacggcgtac tgcgtggcgc ttccggtggt
     3421 cactgcgata ccgcgattgc ctctgcgctt tccatcatcg tcgcgccact ggtacgcggt
     3481 cgtattccga ctctggtgga taacgtgctg acctgcatca ccccgggctc cagtgtcgat
     3541 attctggtca cagaccacgg tatcgcagtt aacccggcac gtccggaact agcagaacgt
     3601 ctgcaggaag cgggcattaa agtggtttcc attgagtggc tgcgcgaacg tgcgcgtctg
     3661 ctgaccggtg aaccacagcc gattgaattc accgaccgcg tcgttgccgt tgtgcgttac
     3721 cgcgatggct cggtgatcga tgttgtgcat caggtgaagg aataagccat gcacctgctt
     3781 cctgaactcg ccagccacca tgcggtgtca attcccgagc tgctcgtcag ccgggatgaa
     3841 aggcaagcac ggcaacacgc ctggctcaag caccatcctg ttccactggt ctcctttacc
     3901 gtggttgcgc ctgggccgat taaagacagc gaggtcacac gccgaatttt taatcatcgc
     3961 gtgacagcct tgcgtgcctt agccacaaag cagggctggc aaattcagga gcaggctgca
     4021 ctggtttccg ccagcgggcc ggagggcatg ttgagcattg ccgccccggc tcgcgacctc
     4081 aagctcgcca ccattgagct tgaacatagt catcctctcg ggcggttatg ggatatcgat
     4141 gtcctgacgc ccgaaggcga aattctctcc cgccgcgact attcactgcg cctcgccgct
     4201 gcctgttgtg cgagcaaagc gcagccgtct gcgcgcgtgg aaaaacccat caactgaccg
     4261 atttactcaa ccgcatggag gcactgctga acgatgtcga tgcctgcaac gtcaactaaa
     4321 accacaaagc ttgcgacgtc attaatcgat gagtacgccc tgctgggctg gcgcgccatg
     4381 ctgactgaag tcaatctgtc accgaaacca ggcctcgtgg atcgcattaa ctgcggtgcg
     4441 cacaaagata tggcgctgga agatttccac cgcagcgcgc tggcgattca gggctggtta
     4501 ccccgtttca ttgaatttgg tgcctgtagt gcagaaatgg caccggaagc ggtactccac
     4561 ggattacgcc caattggtat ggcttgcgaa ggtgatatgt tccgcgccac tgcgggcgta
     4621 aacacgcata aaggcagcat tttttcttta gggctgctat gtgcggcaat tggccgtttg
     4681 cttcagctca accagccggt tacgccaaca accgtttgtt ctacggcggc aagtttctgc
     4741 cgtggcctga ccgatcgcga actgcgtacc aataattcac gactgacggc aggtcaacgg
     4801 ttgtaccagc agcttggcct taccggcgca cgcggtgaag ccgaagcagg ttatccactg
     4861 gtgatcaacc acgccttgcc gcattacctc actctgctgg atcaggggtt agatcctgaa
     4921 ctggcattgc tcgatacctt gctcctactg atggctacca acggcgatac caacgttgca
     4981 tcgcgcggtg gcgagggggg cctgcgctgg ctacagcgcg aggcgcaaac attattgcaa
     5041 aaagggggca ttcgaacccc caccgatctc gattatctcc ggcagttcga cagggagtgt
     5101 atcgaacgaa atctcagtcc aggcggcagt gccgacctgc tgatccttac ctgtttttta
     5161 gcacagattt aattatttaa gcacttgata aatttggaaa tattaatttt cggagaaccc
     5221 gtatgtcttt agcaaaagat aatatatgga aactattggc cccactggtg gtgatgggtg
     5281 tcatgtttct tatccctgtc cccgacggta tgccgccgca ggcatggcat tacttcgctg
     5341 tgtttgtggc aatgattgtc ggcatgatcc tcgagccaat tccggcaaca gcgatcagtt
     5401 ttattgcggt tactatttgc gttattggca gtaattacct gctctttgat gccaaagaat
     5461 tagctgaccc agcgtttaat gcgcaaaaac aggcgctgaa atggggcctg gctggttttt
     5521 ccagcaccac ggtatggctg gtatttggcg catttatttt tgcattaggg tatgaagttt
     5581 ccgggttagg tcgtcgcatt gcccttttcc tggtgaaatt catggacaaa cgcacgctga
     5641 cgttgggtta tgcgattgtc attatcgaca ttctgctggc accgtttaca ccgtccaaca
     5701 ccgcgcgtac cgggggtacg gtttttccgg tcattaaaaa cctgccgccg ttgtttaaat
     5761 cattcccgaa cgatccgtcc gcgcgtcgta ttggcggcta tttgatgtgg atgatggtca
     5821 ttagtaccag tctgagttcg tccatgtttg tcaccggtgc ggaccaaacg tgctgggtct
     5881 ggagttcgtc agcaaaattg ccggtatcca gattagctgg ttgcagtggt tcctctgctt
     5941 cctgccggtt ggggttatct tgcttatcat tgcgccgtgg ctttcctacg tgctgtacaa
     6001 accggaaatc acacacagtg aagaagtggc aacctgggcg ggtgatgaac taaaaaccat
     6061 gggtgcgctg acacgcagag agtggacgct gattggcctt gtattgctca gcttaggttt
     6121 gtgggtattt ggcagtgaag tcattaatgc tactgcggtt ggtctgctgg cagtttcgct
     6181 aatgctggct ctgcacgttg tgccgtggaa agacattacc cgctataaca gcgcatggaa
     6241 cacgctggtc aacctggcaa ctctggttgt gatggctaac ggcctgactc gttctggttt
     6301 tattgactgg ttcgccggta ccatgagtac gcacctggaa ggattctcac caaacgcaac
     6361 ggtgattgta ctggttctgg tgttctactt tgcacactac ctgtttgcca gcctgtctgc
     6421 gcacaccgca accatgctgc cggttattct ggccgtcggt aaaggtattc cgggcgtacc
     6481 aatggaacaa ctgtgtatcc tgctggtgct gtctatcggt atcatgggct gtctgacgcc
     6541 gtatgcaacc ggtcctgggg tgattattta cggctgtggc tatgtgaaat caaaagatta
     6601 ctggcgtctt ggcgcaatct tcggggtgat ttacatctct atgttgctgt tggttggctg
     6661 gccgattctc gccatgtgga actaatacgt tcgccaaacg ctgaataaat catcacgccc
     6721 gccaggtaag cccacctggc gggctttttt atgatttaat agatagtatt cagtgtatgt
     6781 atgagttcca cacccattat gaaagcattc tgccgtaacg ccgcgttgct cgcggtttct
     6841 ctgcttccct tatcttctgc caacgccgta gcgttgcagg caaaacagta tggcgatttt
     6901 gatcgctatg tcctggccct ctcctggcaa accggatttt gccagagtca acacgatcga
     6961 aatcgtaacg aacgagatga atgtcgcctg caaaccgaaa cgaccaacaa aactgatttt
     7021 ctgaccgtac atggtctgtg gccaggattg cctaaatcgg ttgctgcccg tggtgttgat
     7081 gaacgccgct ggatgcgctt cggttgcgct actcgcccaa tcccgaatct accagaagca
     7141 cgcgccagcc gaatgtgttc atcgccggaa accgggttat cactggaaac ggccgctaaa
     7201 ctaagtgaag tcatgccagg agctggcgga cgttcctgcc tggaacgcta cgaatatgcc
     7261 aaacacggtg cctgctttgg ttttgatccg gacgcatact tcggtacgat ggtacgcctg
     7321 aatcaagaaa ttaaagagag cgaagccgga aaattccttg cggataatta cggtaaaaca
     7381 gtgagccgcc gtgactttga cgccgccttt gccaaaagct ggggaaaaga gaacgtgaaa
     7441 gcagttaagc taacgtgcca gggtaaccct gcgtatttga ctgaaattca gatctcgatc
     7501 aaagctgacg ccatcaacgc tccgctttct gcaaactcat tcttgccaca acctcaccca
     7561 ggtaactgtg gcaaaacctt tgtgattgat aaagcgggtt attaatcctc tgatttacaa
     7621 aacacgctaa ttcgtcttcc ataaccatgc cgattaagaa ttatacataa tgcgctgcat
     7681 tgaggaattc cctgaatgtg acgcaaatca cttcaaagag tgagcaacag tcagtatcat
     7741 catgtgagtt aaaccctcgc cgcctgacgg tgagggtttt cttttgggta tgtttatctg
     7801 cgacactcgc agtaccgacg acatggagta aaaatgtcca gaccaactat catcattaac
     7861 gacctggatg ccgaacgcat cgatattctg ctggagcaac ccgcctatgc tggtttgcca
     7921 atcgccgacg cgttaaacgc agagttggat cgcgcccaaa tgtgttcgcc agaagagatg
     7981 ccacacgacg tggtgacaat gaacagccgg gttaaattcc gcaatcttag cgatggcgaa
     8041 gtgcgtgtgc gcacgctggt gtatccggca aaaatgaccg atagcaatac tcagctttcc
     8101 gttatggctc cggtaggtgc cgcactgctg gggctgcgcg ttggcgattc cattcactgg
     8161 gaacttccgg gcggcgttgc aacccacctt gaagtgctgg aactcgaata ccagccagaa
     8221 gctgctggcg actacctgct ttaatcccat ctgcacaagc gataactcgc tttgcccaga
     8281 ggatgcactc gcatcctctt tacgcaaaat cagcaatttt tcctggttct cactgttcac
     8341 ttttgctgca aaattattcc gtttcgtttg tgggaataac catggcagaa gcattttaca
     8401 tcctgatagg atttctgatt atggcggcga ttatcgtcat ggctgtcctt tacctcgaaa
     8461 accactcctg acgatcatat tgttcccccc ggcatcgcat tcaccagacc tgaaaccgcc
     8521 ttctgcgccg cctctgcgct ttgtgcaccg ggtaccagaa tcaccttacg gcactcctct
     8581 tcacgttttt cgaaaatctc atatccccgg gcggcctctt caaacggcat atagtgggta
     8641 acaatttctt ctggtttcag taatcctttc tcaattaacg gtaataattc tcccagccat
     8701 gcgtgaacgt gggtctgtcc cattttaaac gtcaaccctt tatcaaaggc gtcgccaaac
     8761 aggaaaccgt gaataaatcc agcgtagacg cccggtacgc taacaatgcc gcaacgcttt
     8821 accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc tgcctttcgc
     8881 ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg tttgttcaat
     8941 aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc cgtagcggtc
     9001 agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt ccgcaccgag
     9061 caaccgtgca caggcgattg tcaacaatcc cacaggacca gcaccataga ctgcaacgct
     9121 tgaaccttgt tggatctgca cattttttgc tgcctgccat gccgttggca gaatatcaga
     9181 aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc ccacattccc
     9241 tttcgggacg cggacatatt ccgcctgccc gccaggaacg ccaccataca ggtgactata
     9301 accaaacaat gccgcgggag caggtatctg ttttttattg agcgcagcgc ctttacccgc
     9361 attggtattt tcgcaggcgg catattgctg taatcgacag aaaaaacagt cgccacaagc
     9421 aatgacgaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt ccttcccggt
     9481 ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa cctggggtat
     9541 tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttaccgtaa tacgcagaat
     9601 aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa cgtgatgtgg
     9661 gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa gagtcagtct
     9721 ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg agaattccta
     9781 gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga accagacaaa
     9841 tatcagggat aaatattata atcatggaga atgatttgtt catgattaac agggagaaag
     9901 ggttatgtat aaggcaatca ttatgccagt tgatgtattt gaaatggaat tgagcgacaa
     9961 agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt
    10021 actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc gtcgttttga
    10081 agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc acttcaccat
    10141 cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg aagtcaatga
    10201 gttggcagaa gaactggggg ctgatgttgt agttatcggt tctcgcaacc catcgatttc
    10261 gacccatctg ttaggttcta acgcctcgag cgtgatccgc cacgccaatc tgcctgtact
    10321 ggtcgttcgc taacagataa aagtatttat gccgccctgc ctggaacatc ccggcagggc
    10381 tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttgcaggtga
    10441 atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg
    10501 ctttggcacc ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc gtacagtcac
    10561 ccgctgcgaa cacgcctttg acgttggttt cacatttcgc atcaatgata atctcgccca
    10621 tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga ccaatctgga
    10681 caaaaatacc ggccagttcg atgttgtgaa tattgccgct gacacgatct cgatattcca
    10741 gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt
    10801 cgacgttttt caggctgcgc agtttgtcct gcagaacctg gtcggctttc atttctggcg
    10861 caaattccag cagcgttacg tgctcaacga tacccgccag gtcgattgcc gcttccacgc
    10921 cggagttacc gccgccgata accgctacgc gtttgccttt aaacagcggg ccgtcgcagt
    10981 gcgggcagta ggtcacgcct ttagtgcgat actgatcttc gcccggaacg ttcatgttgc
    11041 gccattttgc acccgtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg
    11101 tttcaatctg gtgtagacca ccttcaaccg ctgccgggat cagtttgctg gcgctctggc
    11161 tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct gccaacttct
    11221 gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac
    11281 caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg
    11341 ccgctgcacc cgccgggccg gaaccaacga ttaatacgtc ataagcatca cgcttgttca
    11401 gctcttctgc cgcacgattt tccgcgccag tatcaatttt ggcaacgatt tcagtcaacg
    11461 tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg cccatcacgt
    11521 tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg
    11581 ggttcaatac gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta tggcaagaga
    11641 gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag
    11701 actgcgcttc tttcgacgga tgaccaccgg tccacagcaa cgccagtacc agcgaggtga
    11761 actcgtggcc cagcggggag cctgcaaaac gtggtccctg gttggaacct gggttggtga
    11821 tcaggaaaga cggcttacgc actggcaagc tgttatcttc tttaaaggtg actttgtctg
    11881 acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca
    11941 gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt
    12001 tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct
    12061 ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc
    12121 acccgaagaa ttaggtgaat ttccgaagta tttcgcgtca gagcaaggcg actagcctgt
    12181 gaatccccgg gagcttacac aagtaagtga ccggggtgag cgggcgacgc caacgccgct
    12241 atggcgtgaa agacgaagga aatttagatt ttaccaacca ggtccagaga cggagccaga
    12301 gttgcttcac cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac
    12361 tgagctgctt tgattttacg cagcagatca gacgcgtcac ggccaatgcc ttcagcggta
    12421 acttcgattg cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga
    12481 ccttcatctt cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc
    12541 atcgcatatt tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag
    12601 tgagtatcgg tagatactgc gtatacgtct acgcccagtt tctgcagttc ttcgtagtgg
    12661 tcagcaacgt cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag
    12721 aagacgctcc agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg
    12781 aatgcctggt ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc
    12841 gtgttttcga tgagatgtaa ggtaaccaat ttttgccgat tcggctaatg cgtttccatt
    12901 atcaaatcga taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg
    12961 ccagcggcga taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc
    13021 ctttggtatc ttcagcgcct gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg
    13081 acatggatta caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat
    13141 gacagtgata ggttatgcct tttactcgac ttttgcactg actgaaaaga acaaattaat
    13201 gttaaaaaag atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc
    13261 ttccccagta aaagcgattg aaaaacaggg cattacaatc atcaaaacgt tcgatgcccc
    13321 cggaggaatg aaaggttatc tcggaaagta tcaggatatg ggcgtcacca tctacctgac
    13381 tcccgatggt aagcacgcta tctctggtta catgtacaac gagaaaggtg aaaacctgag
    13441 taacacactt atcgaaaaag aaatttacgc accagccgga cgcgaaatgt ggcaacggat
    13501 ggaacaatcc cactggctcc tcgacggtaa aaaagatgcg ccggtcattg tctacgtctt
    13561 ctccgatccg ttctgcccat attgtaaaca gttctggcag caggcgcgcc cgtgggtaga
    13621 ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc
    13681 gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatatgaagc
    13741 ctctggttgc aagcttaagc taaacgtgcc tgcaaacgta agtacagagc aaatgaaagt
    13801 gttaagtgac aatgagaaac tgatggacga tctgggggca aatgtcacgc cggctatcta
    13861 ttacatgagt aaggaaaata cgctgcaaca ggccgtgggg ttgcccgatc agaaaacgct
    13921 taatatcatt atgggaaata aataaaaggt aattcatgga tggttgaact atatccctgc
    13981 ttttcaacca tcctaaaaaa gcatgaattt ctcgctctac cctaagtggc tgtgctattt
    14041 tagaaaaact taatcctgag ttaagcaaaa aaacataatc aataaaaata catacgcaaa
    14101 aaacattgat taagtgaata taccatggaa gaaaaatata aaccggagta gtgtatggcc
    14161 aatctctacg acttgaaaaa gttcgactta aatcttcttg tcatatttga gtgtatttac
    14221 cagcatttaa gcattagcaa agctgctgaa tcactatata tcaccccatc agccgtgagc
    14281 cagtcgttac aacgcttacg tgcgcaattt aatgaccctt tattcattcg ctcaggaaaa
    14341 ggtatcgcgc cgacgacaac agggttgaat ctgcatcacc atcttgagaa aaacctcaaa
    14401 ggccttgaac aaacaattaa tatagtcaat aagtccgaac taaagaaaaa ctttattatc
    14461 tacggccctc agctcatttc ttgctctaat aatagtatgc tgatccgctg cctacggcaa
    14521 gatgcctctg tcgagattga gtgccatgat atcctcatgt cagcagagaa tgctgaagaa
    14581 ctgttggttc accgcaaagc cgatctggtg atcacccaaa tgccagtgat cagccgttcc
    14641 gtcatttgca tgccattgca tacaattcgc aatactctaa tttgtagtaa taagcatccg
    14701 cgaattactg acaatagcac ttatgaacaa attatggcag aagagttcac ccaattaata
    14761 gccaaatcag caggagttga tgatattcaa atggaaatag acgagaagtt tatgaatcgc
    14821 aaaatttcat ttcgtggttc ctctttgctg acaataatta atagcattgc agttacagat
    14881 ttactgggaa tagtccccta tgaactttat aactaccacc gtgactttct taatttaaaa
    14941 gagataaaac tagaacatcc gttaccttca ataaagcttt acatttccta taataaatct
    15001 tctttaaata atttggtgtt ctcccgattt attgaccgtc taaatgagag tttttgatat
    15061 ataactatag acagctacta taactttcat ctatttattc acagcgcgaa tgaaaaataa
    15121 aacactctgc tttacagtgt ttactttata attaacagtc ttatctcact gttcaccctg
    15181 taacgttcag agagcgtctt cagatgtcta tttataaaat tcctcttccg ctcaatattc
    15241 tggaagctgc acgagagcgt attacctaga cactcaacac cctaccccgc gtttgtgtct
    15301 cattttctgg cggcaaagac tctggtttaa tgctgcatct gacagccgaa cttgcccgac
    15361 aaatgggcaa aaaaatctgc gttttgttta tcaactggga ggc
//
LOCUS       DAJTUP010000091        15161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTUP010000091 DAJTUP010000000
VERSION     DAJTUP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.091"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..933)
                     /gene="anmK"
                     /locus_tag="OO468_003018"
     CDS             complement(<1..933)
                     /gene="anmK"
                     /locus_tag="OO468_003018"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR6260049.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLILGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLL"
     gene            1207..1674
                     /gene="slyB"
                     /locus_tag="OO468_003019"
     CDS             1207..1674
                     /gene="slyB"
                     /locus_tag="OO468_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR6260050.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(1721..2155)
                     /gene="slyA"
                     /locus_tag="OO468_003020"
     CDS             complement(1721..2155)
                     /gene="slyA"
                     /locus_tag="OO468_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR6260051.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2356..2592
                     /locus_tag="OO468_003021"
     CDS             2356..2592
                     /locus_tag="OO468_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR6260052.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2595..3452
                     /locus_tag="OO468_003022"
     CDS             2595..3452
                     /locus_tag="OO468_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6260053.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3452..5464
                     /locus_tag="OO468_003023"
                     /pseudo
     CDS             3452..5464
                     /locus_tag="OO468_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5465..5986)
                     /gene="sodC"
                     /locus_tag="OO468_003024"
     CDS             complement(5465..5986)
                     /gene="sodC"
                     /locus_tag="OO468_003024"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR6260054.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6067..6963)
                     /locus_tag="OO468_003025"
     CDS             complement(6067..6963)
                     /locus_tag="OO468_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR6260055.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7012..7251)
                     /locus_tag="OO468_003026"
     CDS             complement(7012..7251)
                     /locus_tag="OO468_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR6260056.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7354..7953
                     /gene="nemR"
                     /locus_tag="OO468_003027"
     CDS             7354..7953
                     /gene="nemR"
                     /locus_tag="OO468_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR6260057.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            7990..9087
                     /gene="nemA"
                     /locus_tag="OO468_003028"
     CDS             7990..9087
                     /gene="nemA"
                     /locus_tag="OO468_003028"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR6260058.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9168..9575
                     /gene="gloA"
                     /locus_tag="OO468_003029"
     CDS             9168..9575
                     /gene="gloA"
                     /locus_tag="OO468_003029"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR6260059.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            9678..10325
                     /gene="rnt"
                     /locus_tag="OO468_003030"
     CDS             9678..10325
                     /gene="rnt"
                     /locus_tag="OO468_003030"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR6260060.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10418..12532
                     /locus_tag="OO468_003031"
     CDS             10418..12532
                     /locus_tag="OO468_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR6260061.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12542..13834
                     /locus_tag="OO468_003032"
     CDS             12542..13834
                     /locus_tag="OO468_003032"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260062.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            14784..14876
                     /locus_tag="OO468_003033"
     CDS             14784..14876
                     /locus_tag="OO468_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR6260063.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(14920..>15161)
                     /locus_tag="OO468_003034"
     CDS             complement(14920..>15161)
                     /locus_tag="OO468_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6260064.1"
                     /translation="AVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGA
                     RNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3462 a         4011 c         3990 g         3698 t
ORIGIN      
        1 cagtaatacc gccagacgcg ccatgagtag cggattacga ctacctccac cacataccat
       61 caatcgttcg cagccaccgc tcaacaaaac ttgttcagaa atggtcacgg cggtgagttc
      121 tgccagtgtg gcctgaacat cacggggatc aacacccgga aaatggcgta aatggcgctc
      181 cagccagccg tagttaaagt attcgcgtcc ggtgcttttc ggtgcaggtt gcgagaaata
      241 cgggtcgctg agcatatttt gcagcagtgg gagaataact ttacccgccc gtgcccactc
      301 ggcatcttta tcgtaaggtt tacctgcctg acgccagatc caggcatcca tcagcatgtt
      361 accaggaccg gtatcgtagc cgccaaccag ctgcccaaga atgagcagtg acagattggc
      421 gatgccgcca atattgagca ccattcgtcg ctcggttggg tgagccagca aggcatgatg
      481 gaacgcaggt accagcggtg cgccttgtcc tcccaaggca atatcgcgac ggcgaaaatc
      541 accgacaacc gtaattccgg tgcgtgccac aatttgattg ttatcgccaa tctgtaaagt
      601 gtgtggtgcc acgcccgtcg gttcatgcca gacggtttga ccgtgacagc cgatcgcaac
      661 tatatctctc gcctgcaggt tttgttcctt aagcaaggca ttgaccgcat cagcaaaaag
      721 tcgcccgagt tgagtatcaa gctgtccaaa ctgcgaaagt gtaagctgct ggccctggca
      781 aatatccagt acagcctgtt tcagagacac cgggatcggc cagctcaagc ttgccagctg
      841 tgcgacccgg tgttcatcaa ttgtcgccaa cacaacatca acaccatcaa ggctggtgcc
      901 tgacataacg ccaataaagc ggcccgattt catagttcat cctttttcaa tctgacgttt
      961 gcgcaccact caaacataaa cttttcgtga ataccatgcg gaatgaccga tttttaccgt
     1021 tggtagtaaa acattatctt caaatcaata atcatcatga atgttttgtt tataattggt
     1081 tgatcctact ttcattatga tttgctcata tttggtagaa catgtaacca tggattcaca
     1141 tatgccatat actttgacca tgagggatgc ttgcgtggcg tttcatggtg aacaggagat
     1201 ttttcaatga ttaaacgcgt attggttgtt tcaatggtag gtctgtctct tgtcggttgt
     1261 gttaataacg acaccctgtc aggggatgtt tataccgctt ctgaagcgaa acaagtacag
     1321 aatgtcagct atggcaccat cgttaacgta cgtccggtac agattcaggg cggtgatgat
     1381 tccaacgtta tcggtgcaat tggcggtgct gttcttggtg gtttcctggg gaatactgtt
     1441 ggtggcggaa ccgggcgttc tctggctact gcagcaggcg ctgttgcagg tggcgtagct
     1501 ggtcagggcg tacagagtgc aatgaacaaa acgcagggtg tcgagctgga aattcgtaaa
     1561 gacgatggta ataccatcat ggtggtacag aaacaaggca acactcgttt ctctccgggc
     1621 caacgtgtcg tactggccag caatggcagt caggtgaccg tttctccgcg ctaaataagt
     1681 ttgcgtgtgg tcaggttact gaccacacgc ccccttcatt tcaccctttg gcctgtaact
     1741 caatgatatt atgctcaagt tttgcgatga gcgtaatcag ttgctccagt tcctctgcgg
     1801 agatgccatg taatatttcc gcgcgggttt tgttaataac agcttccatt tcgctgatca
     1861 gtggctctgc cttttccgtc agtttaatac gtttagcccg acgatcgctg gcacaagttt
     1921 gacgcgaaat taaccctttt tcttccagtt ggtccagagt acggaccagt gatggctgct
     1981 cgatgccaat cgcttttgcc agttgaattt gcgactggtc tggaggtaac tgatggatat
     2041 tgtgtaacgt aacccaatgg gtttgtgtta actccagcgg tttcaggcga tggtctatca
     2101 gagcacgcca tatgcgcacc aaccgtgcca gatcagaacc tagtggcgat tccaatttca
     2161 tctccttata attagcttgc taagatatta tgcggctttt agaatagtgt gcagcaattg
     2221 tattgctaaa acaaatgtat tgctgcattt ggttaccgtc agacatattt ttcagaaatt
     2281 gcgcgtaaat ttttcgcact taaagaatat ttattaatct aacgcaatat attcggtcgt
     2341 aaaggaatct actttgtgaa gtttatgctc aatgcaacag gattgccctt acaagatctg
     2401 gtgttcggtg cgtccgtcta ttttcctccg tttttcaaag cattcgcgtt tggattcgtc
     2461 atctggcttg tcgtacaccg cctgcttcgt ggctggatct acgccggcga tatctggcat
     2521 cccttgttaa tggatttatc gctgtttgcg atttgcgttt gccttgctct ggcaatactg
     2581 attgcgtggt aactatgtca attaaaacaa ttaagtattt ctcaacaatc attgtagcga
     2641 tagttgcggt tcttgccgga tggtggctgt ggaattatta catgcaatct ccgtggacac
     2701 gcgacgggaa aatacgcgca gaacaggttt ctatcacccc acaggtgtcg ggacgtattg
     2761 ttgagctgaa tataaaagac aaccaactgg ttaacgcggg ggaacttctg ctcacgatcg
     2821 ataaaacgcc ctttcagatc gccgagctga acgcacaggc tcagctggcg aaagcgcaat
     2881 ctgacctcgc aaaagccaat aacgaagcga atcgtcgtcg tcatctctca caaaatttta
     2941 tttttgccga agaactcgat accgctaacc tcaatgttaa agcgatgcag gccagtgtta
     3001 atgccgcaca ggcgacgctg aaacagacac aatggcaact ggcgcaaacg gaaattcgcg
     3061 ccccggtgag tggatgggtg actaacctca ctacccgcat cggcgattac gctgacaccg
     3121 ggaaaccgct gtttgccctt gtcgacagcc actcgtttta cgtcattggt tattttgaag
     3181 aaaccaagtt gcgccatatc cgcgaaggtg caccagcaca aattaccctc tatagtgata
     3241 acaaaacgtt acagggtcac gtttccagta tcggtcgggc gatttatgat cagagcgttg
     3301 aaagtgactc aagccttatt ccggatgtga aacctaacgt cccctgggtt cgtctcgccc
     3361 aactcgttcc cgtccgcttt gcgctggata aagttccagg cgatgtcacg ctggtgtcgg
     3421 gcaccacctg tagcattgcc gtaggtcaat aatgaacgca tcgtcatggt ccttgcgcaa
     3481 tttgccctgg ttcagggcca cgctggcgca atggcgttat gcgttacgca ataccattgc
     3541 catgtgtctg gcgctgacgg ttgcctatta tttgaatctg gatgaaccct attgggcgat
     3601 gacctcggct gcagtggtta gctttcccac cgttggcggt gtcatcagca aaagcctcgg
     3661 acgcatggct ggcagtttac tgggagccat tgcggcactg cttcttgccg gacatacgct
     3721 caatgagccg tggttttttc tattgagcat gtcggcgtgg cttggctttt gtacctaggc
     3781 ctgtgcgcac ttcacgaata acgtcgcgta tgcatttcaa ctggcgggct acacggctgc
     3841 catcatcgcc tttccgatgg ttaatattac tgaggccagc cagctgtggg atatcgctca
     3901 ggcgcgcgtt tgcgaggtga tagtcggtat tttgtgcggc ggcatgatga tgatgatcct
     3961 gcctagcagt tccgatgcta ctgccctttt aactgcattg aaaaacatgc acgcccgatt
     4021 actggaacat gccagtttac tctggcagcc tgaaacaaac gatgccattc gtgcagcaca
     4081 tgagggggtg attgggcaga tactgaccat gaatttgctg cgtatccagg ctttctggag
     4141 ccactatcgt tttcgccagc aaaacgcgcg ccttaatgcg ctactccacc agcaattacg
     4201 tatgaccagt gtcatctcca gcctgcgacg tatgttgctc aactggccct caccgccagg
     4261 tgccacgcga gaaattctcg aacaattgct gacggcgctc gccagttcgc aaacagatgc
     4321 ttacaccgtc gcacgtatta tcgccccgct acgcccgacc aacgtcgccg actaccggca
     4381 cgtcgccttc tggcagcgac tacgttattt ttgccgcctt tatctgcaaa gtagtcagga
     4441 attacatcgt ctgcaaagcg gtgtagatga tcatgccaga ctcgcacgga catccgctct
     4501 ggctcgtcat accgacaacg ccgaagctat gtggagcggg ctgcgtacat tttgtacgtt
     4561 gatgatgatt ggcgcatgga gtattgcatc gcaatgggat gccggtgcca atgcattaac
     4621 gctggcagca attagctgcg tactctactc cgccgtcgca gcaccgttta agtcgttgtc
     4681 actgctgatg cgcacgctgg tgttactttc gctattcagc tttgtggtca aatttggcct
     4741 gatggtccag attagcgatc tgtggcaatt tttactgttt ctctttccac tgctggcgac
     4801 aatgcagctt cttaaattgc agatgcccaa atttgccgca ctgtgggggc aactgattgt
     4861 ttttatgggt tcgtttatcg ctgtcactaa tcccccggtg tatgattttg ctgattttct
     4921 taactataat ctggcaaaaa tcgttggcgt tgcgctggct tggctagcgt tcgccattct
     4981 gcgtccagga tcggatgctc gtaaaagccg ccgccatatt cgcgcgctgc gccgggattt
     5041 tgtcgatcag ctaagccgcc atccaacact gagtgaaagc gaatttgaat cgctcactta
     5101 tcatcacgtc agtcagttga gtaacagcca ggatgcgctg gctcgccgtt ggttattacg
     5161 ctggggtgta gtgctgctga actgttctca tgttgtctgg caattgcgcg actgggaatc
     5221 gcgttccgat ccgttatcgc gagtacggga taactgtatt tcactgttgc ggggagtgat
     5281 gagtgagcgt ggcgttcagc aaaaatcact ggcggccaca cttgaagaat tacagcggat
     5341 ttgcgacagc cttgcccgtc atcatcaacc tgccgcccgt gagctggcgg caattgtctg
     5401 gcggctgtac tgctcgcttt cgcaacttga gcaagcacca ccgcaaggta cgctggcctc
     5461 gtaattactt aattacacca caggcatagc gttcaccgcc accgcccagc ggtttaggtt
     5521 gatcggacat attatcgccg ccaacgtgga ccatcagcgc cttgtctttg atttcatcca
     5581 gtgatttcag acgaggcgcg atgacggcat cggtagcttt gccgtcatta ttgacgacca
     5641 gtgcaggcag atcgcctaaa tgcccggtac cttctggccc ttcatgttta ccggtatttt
     5701 gtggatcaag atgcccgcct gcggattccg cggcgctggc tttgccatct ttggtggctg
     5761 gctggcagct tcctttggca tgaatatgga agccatgttc accggggggt aatgctttca
     5821 gatcgggtga aaactccaga cctttatcgg tttcagtaat ggtgacgcta ccaattgact
     5881 gcccaacccc ttgcgacgtg acgaggttca tctcgacttt ttcactggca gcttgtgcgc
     5941 cggtcgcaac aaccagcgcc agaatggcca gactaaaacg tttcatagga cctccgttca
     6001 ttgtgcattc ctgaataagt gtaaaccagt gacacatttt tgaacggcgg ctattcctaa
     6061 aagtgcttac ggtacgtcgt accccagtgc agctttacgg atacgaaacc attgttgacg
     6121 ggtcattttc agtgtttctg cttcgacagc tgcccgtacg cgctcaattt tacctgagcc
     6181 gataattggc agcggctgcg acggtaaacg taatacccag gcgtaaacca cctgttcaat
     6241 cgagcccgcg tttaactcct ccgccaccac agccagttca tcacgcagcg gctggaaata
     6301 atcatcatta aacagacgac caccaccaag gcaggaccac gccatcggac gaacacgcag
     6361 ttgttgtagt tggtcgagcg tgccatccag cagtaacggc tgatgcaccg gggatatttc
     6421 cacctgatta gtggcaaggg taaacggcag acgtgattgc aacagggcaa attgcgcagg
     6481 cgtaaagttc gatacgccaa aatgacgcac tttgccgctc tgatgcagat gtttgaacgc
     6541 gtccgccact tcatcggcat ccattaacgg gtctggtcgg tggattaaca gcagatccag
     6601 atgatcggtc gcgagattaa ttagcgactg ttcggcgctc ttaatgatgt gattgcggtc
     6661 agtgatgtaa tgaccaatga cgttttcttc acgcgcggtc gtcgcgatac cgcatttact
     6721 gacgatttcc atccgttcac gcaggtgagg tgccagtttc agtgcctcgc caaacgccgc
     6781 ttcgcactga tagccaccat aaatatcagc atggtccacg gtggtcacgc cgagatccag
     6841 atgctcttca ataaaactga ccagctggcg ggcggacata ttccagtcca tcaatcgcca
     6901 gtagcccatc acaaaacggg aaaactccgg gccttccggc gcaatagtaa tacgctgaac
     6961 cataatcgct tcctcttatc agatataaga ggagtatacg caagattagg ttcaaaagag
     7021 tgatggttgc tccggttcgt ctgatgacgc tggcttattt gcgcgtaatt tgcgcattaa
     7081 tcgctgccga caaaggcgca gcacctcttg tttttcgcca tcgctcattt tattccagtt
     7141 aaaacgctca tcccgactac gaaaacagcc gcgacaaaac ccgcgttcgt cagactggca
     7201 aattccccgg cacgggctct ggacgggaaa gaactctaat tgctccgcca cctcgccctc
     7261 ctcagataag attattacca ttattgaagc tgttaatgtc caaagtagca actttgcttg
     7321 cactagacca actggtctac tacactccaa cgcatgaaca aacacaccga acatgatact
     7381 cgcgaacatc tcttggcgac gggcgagcaa ctttgcctgc aacgtggatt caccgggatg
     7441 gggctaagcg aattactaaa aaccgctgaa gtgccgaaag ggtccttcta tcactacttt
     7501 cgctctaaag aagcgtttgg cgttgccatg cttgagcgtc attacgccgc atatcaccag
     7561 cgactggctg agttgctgca atccggcgaa ggtaactacc gcgaccgcat actggcttat
     7621 taccagcaaa cactgaacca gttttgccaa catggaacca tcagtggttg cctgacagta
     7681 aaactctctg ccgaagtgtg cgatctgtca gaagatatgc gcagcgcgat ggataaaggt
     7741 gctcgcggcg tgatcgccct gctctcgcag gctctggaaa atggccgtga gaaccattgt
     7801 ttaacctttt gtggcgaacc gctacaacag gcacaagtgc tttacgcact atggcttggc
     7861 gcgaatctgc aggccaaaat ttcgcgcagt ttcgagccac tggaaaacgc gctggcccat
     7921 gtaaaaaaca ttattgcgac gcctgccgtt tagcaggcat tttttatcac cagacgaccg
     7981 ggagccttta tgtcatctga aaaactgtat tccccactga aagtgggcgc gatcacggcg
     8041 gcaaaccgta tttttatggc accgctgacg cgtctgcgca gtattgaacc gggtgacatt
     8101 cctaccccgt tgatggcgga atactatcgc caacgtgcca gtgccggttt gattattagt
     8161 gaagccacgc aaatttctgc ccaggcaaaa ggatatgcag gtgcgcctgg catccatagt
     8221 ccggagcaaa ttgccgcatg gaaaaaaatc accgctggcg ttcatgctga aaatggtcat
     8281 atggccttgc agctgtggca caccggacgc atttctcacg ccagcctgca acctggcggt
     8341 caggcaccgg tagcgccttc agcacttagc gcgggaacac gtacttctct gcgcgatgaa
     8401 aatggtcagg cgatccgcgt tgaaacatcc atgccgcgtg cgcttgaact ggaagagatt
     8461 ccgggtatcg tcaatgattt ccgtcaggcc attgctaacg cgcgtgaagc cgcttttgat
     8521 ctggtagagc tccactctgc tcacggttat ttgctgcatc agttcctttc cccttcttca
     8581 aaccatcgta ccgatcagta cggcggcagc gtggaaaatc gcgcacgttt ggtactggaa
     8641 gtggtcgatg ccgggattga agaatggggt gccgatcgca ttggcattcg cgtttcgcca
     8701 atcggtactt tccagaacac agataacggc ccgaatgaag aagccgatgc actgtatctg
     8761 attgaacaac tgggtaaacg cggcattgct tatctgcata tgtcagaacc agattgggcg
     8821 gggggtgaac cgtatactga tgcgttccgc gaaaaagtac gcgcccgttt ccacggtccg
     8881 attatcggcg caggtgcata cacagtagaa aaagctgaaa cgctgatcgg caaagggtta
     8941 attgatgcgg tggcatttgg tcgtgactgg attgcgaacc cggatctggt cgcccgcttg
     9001 cagctcaaag ctgagcttaa cccacagcgt gccgaaagtt tctacggtgg cggcgcggaa
     9061 ggctataccg attacccgac gttgtaatcc aacattgcga gcggcgtaaa gccgccgcta
     9121 tactaaaaca acattttgaa tctgttagcc attttgagga taaaaagatg cgtcttcttc
     9181 ataccatgct gcgcgttggc gatttgcaac gctccatcga tttttatacc aaagtgctgg
     9241 gcatgaaact gctgcgtacc agcgaaaacc cggaatacaa atactcactg gcgtttgttg
     9301 gctacggccc ggaaaccgaa gaagcggtga ttgaactgac ctacaactgg ggcgtggata
     9361 aatacgaact cggcactgct tatggtcaca tcgcgcttag cgtagataac gccgctgaag
     9421 cgtgcgaaaa aatccgtcaa aacgggggta acgtgacccg tgaagcgggt ccggtaaaag
     9481 gcggtactac ggttatcgcg tttgtggaag atccggacgg ttacaaaatt gagttaatcg
     9541 aagagaaaga cgccggtcgc ggtctaggca actaatctcc tgccgggcgt gaactcatcg
     9601 cgcccgcatc tttactgcat cgacaagtaa tatttgtcat aatgcgcgct gcaatttatc
     9661 cgtattaaga gaatcagatg tccgataacg ctcaacttac cggtctgtgc gaccgttttc
     9721 gtggttttta tcctgttgtg atcgatgttg aaacagccgg atttaacgcc aaaaccgatg
     9781 cgctgcttga gattgccgcc atcaccctga aaatggatga acaaggctgg ctgatgccgg
     9841 acaccacatt acatttccac gtcgaaccat ttgtcggcgc aaatttgcaa ccagaagccc
     9901 tcgccttcaa cggcattgac ccgaacgatc ccgatcgcgg cgcggtcagc gaatacgagg
     9961 cgctgcacga aatttttaaa gttgtacgta aaggtattaa agcgagcggc tgtaaccgcg
    10021 ccattatggt ggcgcacaac gccaattttg atcacagctt tatgatggcc gccgcagaac
    10081 gcgcctcgct gaaacgtaac ccgttccacc ctttcgccac ttttgacact gctgcactgg
    10141 ccgggctggc actcggacaa accgtattgt caaaggcttg ccagaccgct ggcatggact
    10201 tcgacagcac ccaggcgcac tccgcgctgt acgacaccga acgcactgct gtgctgtttt
    10261 gtgaaatcgt caaccgctgg aaacgtctgg gaggctggcc gctacctgcc gccgaagagg
    10321 tgtaatcgag tcgatgcctg atgacatgca atgattcagg catctatagt gaggctattc
    10381 cacgcatcaa gcatgatatt cacggggaat agcgttaatg gcagataatc cagacccttc
    10441 atcgctcctg ccggacgtgt tttcaccggc gacccgcgac tggtttcttc gcgcctttaa
    10501 acagccgacc gctgtccagt cgcaaacctg gcatgtggcg gcgcgaggcg aacatgcgct
    10561 ggtgattgca ccgactggct ccgggaaaac gctggcagca tttctctacg ccctcgatcg
    10621 gctcttccgc gaaggcggcg aagatacccg cgaggcgcat aagcgtaaaa cctcacgcat
    10681 cctctatatt tcaccgataa aagccctggg caccgacgtt cagcgcaact tgcagatccc
    10741 gttgaagggt attgccgatg aacggcggcg gcgcggcgaa acggtaatta ttgatgaagt
    10801 ccacgcggta gcgggcagta aacgtggtgc gcatctggcg ttaagtctgg agcggctcga
    10861 tgcgctgctc cacacctcag cacagcgaat tggcctttct gccactgtgc gctcagccag
    10921 cgatgtggca gcatttcttg gtggcgatcg cccggttacg gtagtcaacc cgcccacaat
    10981 gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat atggatgatg tctcatcggt
    11041 cgccagcggc accggcgaag acagccatgc cggccgggaa ggctccatct ggccatatat
    11101 tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg accattgtct ttactaattc
    11161 acgcgggctg gcggaaaaac tgacggcacg attaaatgag ctttacgccg cacgcttaca
    11221 gcgttccccg tctatcgccg ttgatgcggc ccatttcgag tcgacctccg gcgcaacctc
    11281 taaccgtgta caaagtagcg acgtttttat tgcccgctca caccacggct ccgtctctaa
    11341 agaacaacga gcaatcaccg aacaggcgct gaaatcgggt gaattacgct gcgtggtcgc
    11401 aacctccagt cttgaactgg ggattgatat gggcgcggtg gatctggtga ttcaggtggc
    11461 aacgccgctt tctgttgcca gtgggttaca acgcattggt cgcgccggac atcaggttgg
    11521 cggtgtatct aaagggctgt ttttcccccg tacccggcgt gatttagtcg attccgcagt
    11581 cattgtagag tgtatgttcg caggcaggct ggaaaacctg acaccaccgc ataatcctct
    11641 cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg gatgcattac aggtagacga
    11701 atggtactcc cgcgtacgcc gtaccgcacc gtggaaagat ctgccaagac gtgtttttga
    11761 cgccacgctg gatatgcttt ccgggcgcta tccctctggc gatttttctg cttttcgccc
    11821 caaactggtc tggaacaggg agaccgggat attgaccgcc cgacctggcg ctcaattgtt
    11881 ggcggttacc agcggcggca ccattccgga tcgtggcatg tatagcgtgt tattacccga
    11941 aggtgaagaa aaggccggtt cgcggcgggt gggtgaactg gatgaggaga tggtatatga
    12001 gtcgcgggtg aacgacatta tcactctcgg cgctacctca tggcggatcc agcaaatcac
    12061 ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct gcccagctcc ccttctggcg
    12121 tggtgaaggt aacggacgtc cggctgaatt aggcgagatg atcggcgatt ttcttcattt
    12181 actggcggat ggcgcgttct tttccgggac tattcccccg tggctggcag aagaaaatac
    12241 gaacgccaat attcaggggt tgattgacga gcaacgcaac gcgacgggca tcgttccggg
    12301 gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt ggcgactggc gtattatttt
    12361 gcactctccc tatggaagac gggtgcatga accctgggcg ctggcgattg ccgggcgaat
    12421 acatgcccta tggggcgctg acgcgtctgt ggtcgccagt gatgacagca ttgttgcgcg
    12481 cattcctgac actgatggta aattgcccga cgccgcgatt tttttgtttt aaccagaaaa
    12541 gttgctgcaa attgtccgcg aggcggtagg cagctcggca cttttcgccg cccgttttcg
    12601 cgaatgcgcc gcgcgggcat tattaatgcc gggccgcacg ccgggccatc gcaccccgct
    12661 ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc gctcagggat atccggattt
    12721 tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat gtttatgatc ttcccgcact
    12781 ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa atatccgatg taacgaccac
    12841 tacgccctcg cctttcgcca caagtttatt gttcggctat gtcgcggaat ttatgtacca
    12901 gagcgacgcc ccgctggcag agcgccgggc atccgtactg tcgctggaca gcgagttact
    12961 gcgcaatcta ctcggacagg tcgatccggg ggaattactc gacccgcagg tcattcgcca
    13021 ggtggaagaa gagttgcaac gactggctcc tggtagaaga gcgaaaggtg aagaaggatt
    13081 gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa gacctggcgc aacggcatac
    13141 aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt cttgcagtaa aacgaatctt
    13201 cccagcgatg attagcggac aggagcgtct tgcctgtatg gatgatgccg ccaggctgcg
    13261 tgatgccctc ggcgtacggc taccagagtc attgccagag atttatttac atagagtcag
    13321 ttacccgctt cgcgacctct ttctgcgcta tctccgggct catgctctgg tcacggctga
    13381 acaactggct catgagttta gtctcggtat tgccattgtc gaagagcagc ttcagcaact
    13441 gcgtgaacag ggtctggtga tgaatctgca acaagacatc tgggtgagcg atgaagtatt
    13501 tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa gcgacgcgtc ccgttgcagc
    13561 cacgacctat gcgcgattgc tgctggaacg tcagggcgta ttacccgcca ccgatggtag
    13621 cccggcgctc tttgcctcaa catctccagg cgtttatgag ggcgtagatg gagtgatgcg
    13681 ggtgatcgaa cagcttgccg gagtcggttt acccgcctca ctctgggaaa gccagatcct
    13741 gcctgcccgc gtacgcgact attcgccaga aatgctcgat gaattactgg caaccggagc
    13801 ggttatctgg tcgggacaaa aaaaagctgg gtgaagatga cggcctggtg gcactgcatc
    13861 tacagaaata tgccgcagaa tcgttcactc ccgccgaagc ggatcaggcg aatcgttcgg
    13921 cgctgcagca agcgataatc gctgttctgg ctgacggagg agcctggttt gcacaacaaa
    13981 tcagccaacg gatacgcgat aaaatcgtcg aatcggttga ttcctcggca ctgcaagagg
    14041 cgttatgggc gctggtctgg caaggcgtca ttaccagcga tatttgggca ccgttacgcg
    14101 ccctcacccg cagcagttcc aacgcacgca cctcaactcg ccgcagtcac cgggctcgtc
    14161 gtgggcgtcc tgtctatgca caacccgtct cgccgcgggt atcttacaac acaccaaatc
    14221 tggctggacg ctggtcgtta ttgcaggtgg agccactaaa cgataccgaa aggatgctgg
    14281 cgctggcgga aaatatgctc gaccgctacg gcatcatcag tcgtcaggcg gtgatagccg
    14341 aaaatatccc tggcgggttt ccatcgatgc aaacactttg tcgaagtatg gaagactccg
    14401 ggcgaattat gcgaggtcgt tttgtagaag gtctgggtgg cgcgcaattc gctgaacgtc
    14461 tgactattga ccgattgcgc gatctggcga cacaagccac gcaaacacgc cactatacac
    14521 cagtggcgct ctccgccaac gatccggcta atgtgtgggg aaatcttctg ccctgggctg
    14581 cacatccggc aacgctggta ccaacgcgtc gggcgggtgc gctggtggtc gtttctggcg
    14641 gcaaattgtt actctatctg gcgcaaggcg gcaaaaaaat gctggtctgg caggaaaagg
    14701 aggaattact cgccccagag attttccagg cgctgaccac cgcactgcgt cgcgaaccaa
    14761 ggctgcgctt tacgctaaca gaagtgaatg atcttccggt ccggcaaacg ccgatgttta
    14821 cgctgctgcg tgaggcggga ttttcaagtt cgccacaagg gctggattgg ggatagttga
    14881 catcctccac gccctgaaag acgtggttcc ctgctacgtt caagctgtcg cctgaatcat
    14941 ttcggtgggt tcctgcttca acgagcggcc tgactgcacc atctctccac aggcaagaac
    15001 ggcgtgcccc gccgctaaaa tgttacgagc gccatttaca tcggcgttcg ctgtatatcc
    15061 acatacctgg catctgaatt tactttgtga caggcggttt tctttcgctg tatgaccaca
    15121 gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca a
//
LOCUS       DAJTUP010000092        14588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTUP010000092 DAJTUP010000000
VERSION     DAJTUP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.092"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..785)
                     /gene="tdh"
                     /locus_tag="OO468_003035"
     CDS             complement(<1..785)
                     /gene="tdh"
                     /locus_tag="OO468_003035"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR6260065.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAML"
     gene            complement(795..1991)
                     /gene="kbl"
                     /locus_tag="OO468_003036"
     CDS             complement(795..1991)
                     /gene="kbl"
                     /locus_tag="OO468_003036"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR6260066.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2266..3123)
                     /gene="yibB"
                     /locus_tag="OO468_003037"
     CDS             complement(2266..3123)
                     /gene="yibB"
                     /locus_tag="OO468_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR6260067.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3427..4359
                     /gene="rfaD"
                     /locus_tag="OO468_003038"
     CDS             3427..4359
                     /gene="rfaD"
                     /locus_tag="OO468_003038"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR6260068.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4369..5415
                     /gene="rfaF"
                     /locus_tag="OO468_003039"
     CDS             4369..5415
                     /gene="rfaF"
                     /locus_tag="OO468_003039"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR6260069.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5419..6411
                     /gene="rfaC"
                     /locus_tag="OO468_003040"
     CDS             5419..6411
                     /gene="rfaC"
                     /locus_tag="OO468_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR6260070.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6408..7616
                     /gene="rfaL"
                     /locus_tag="OO468_003041"
     CDS             6408..7616
                     /gene="rfaL"
                     /locus_tag="OO468_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR6260071.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(7652..8794)
                     /locus_tag="OO468_003042"
     CDS             complement(7652..8794)
                     /locus_tag="OO468_003042"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR6260072.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(8803..9816)
                     /locus_tag="OO468_003043"
     CDS             complement(8803..9816)
                     /locus_tag="OO468_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR6260073.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(9841..10548)
                     /gene="rfaY"
                     /locus_tag="OO468_003044"
     CDS             complement(9841..10548)
                     /gene="rfaY"
                     /locus_tag="OO468_003044"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR6260074.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10574..11581)
                     /gene="waaO"
                     /locus_tag="OO468_003045"
                     /gene_synonym="rfaI"
     CDS             complement(10574..11581)
                     /gene="waaO"
                     /locus_tag="OO468_003045"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR6260075.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(11624..12421)
                     /gene="rfaP"
                     /locus_tag="OO468_003046"
     CDS             complement(11624..12421)
                     /gene="rfaP"
                     /locus_tag="OO468_003046"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR6260076.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12414..13538)
                     /locus_tag="OO468_003047"
     CDS             complement(12414..13538)
                     /locus_tag="OO468_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR6260077.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13535..>14588)
                     /gene="rfaQ"
                     /locus_tag="OO468_003048"
     CDS             complement(13535..>14588)
                     /gene="rfaQ"
                     /locus_tag="OO468_003048"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR6260078.1"
                     /translation="KPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQDT
                     IPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAILV
                     RLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQTTM
                     SYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEVVL
                     TSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHIAA
                     AVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAADVI
                     AAVDKLLPSSTTGTSL"
BASE COUNT         4131 a         3281 c         2999 g         4177 t
ORIGIN      
        1 agcatcgcaa tacgaccgcc gtggttcatg gtatcgagca tggtacgaaa cgctggcggc
       61 gcgccggaca tttccagacc gacatcaaag ccttcggtca tgcccagttc agccatcaca
      121 tcattaaggt tttctttcgc gacgttaacc gcacgggtga tacccatttt gcgcgccagc
      181 tcaaggcggt attcgttaac atcggtgatc accacattgc gtgcaccaac gtgtttcgcc
      241 accgccgcag ccataatacc tatcggacct gcaccagaaa ccagcacatc ttcgcccacc
      301 agatcgaacg acagcgcggt atgcacggcg ttaccgaagg gatcaaaaat ggaagccagg
      361 tcgtcggaga tattgtcggg gattttgaaa gcgttgaacg ccggaatcac cagatattcg
      421 gcgaagcagc ccggacggtt aacaccgaca ccaatggtgt tgcggcacag atgggtacga
      481 ccgccacgac agttgcgaca gtgaccgcag gtgatatggc cttcgccaga aacgcgatcg
      541 ccgattttga agcctttcac ttcctgacca atacctacca cttcaccgac atattcatgg
      601 cccacgacca tcggcaccgg gatggttttt tgcgaccact catcccagtt atagatgtga
      661 acgtcagtcc cgcagatggc tgttttacgg attttaatca gcagatcgtt atgcccgagt
      721 tccggtacag ggacgtcggt catccagatg ccctcttccg ctttcagttt ggataacgct
      781 ttcatctcac atcctcaggc gataacgccc agttgtttac caatacgcgt gaatgcttct
      841 actgcacgcg taatttgctc aggggtatgc gccgcagaca tctgggtacg aatacgcgcc
      901 tgacctttcg gaacgaccgg atagaagaaa ccggtgacgt aaatgccctc tttttgcagc
      961 tctcgggcaa atttctgcgc cactaccgca tcaccaagca tgaccggaat aatggcgtga
     1021 tcggctcccg ccagggtaaa gcccgccgcc gacatttgct cacggaactg acgcgcgttc
     1081 gcccacagac ggtcacgcaa ctcgctaccc gcttcaacca tctccagcac tttgatggaa
     1141 gcggcgacaa ttgctggtgc cagcgagttg gagaacaggt acggacgaga acgctggcgc
     1201 agccactcaa ccacttcttt gcgcgccgcg gtataaccac cagaagcccc gcccagcgct
     1261 ttaccgagcg tgccggtgat gatgtcgaca cgccccatca catcgcagta ttcatgggaa
     1321 ccacgaccat tttcaccgac aaaaccgacc gcgtgggagt cgtctaccat caccagggca
     1381 tcgtatttat ctgccagatc gcaaacgccc ttcagattgg caatcacgcc gtccatcgag
     1441 aacacaccat cggtggcaat cagcacatga cgcgcaccgg cttcacgcgc ttctttcaga
     1501 cgagcttcaa gctcctgcat atcgttgttg gcatagcgat agcgtttagc tttacacaga
     1561 cgcacaccat caataataga cgcgtggttc agtgcgtcgg agataatggc gtcttccgcg
     1621 cccagcagcg tttcaaacag gccaccgtta gcatcaaagc aggaagagta gagaatcgca
     1681 tcttccatcc ccaggaatgc cgccagtttt tgttcaagct ctttatggct gtcctgagtg
     1741 ccgcaaataa aacgcaccga agccatgccg aaaccgtgag aatccattcc cgcctttgct
     1801 gccgcaatca gatccggatg attcgccagc ccgagatagt tgttggcaca aaagttaatg
     1861 acgtggcttc catcagccac ggtgatatct gcttgctgcg cagacgtaat aatgcgctct
     1921 tctttaaaca acccttccgc ccgtgcggtt tccagatcgt tggttaactg ctgataaaat
     1981 tctccacgca ttgcgattct ccagacaggg caaattccag cacatattac ccaaacttat
     2041 aggtcgcgac gagatgatgc gttaacactt ctgcaaaatt caggataaat aacgtgtatc
     2101 ttgatgtctc tcaacaccta cagatagtag aaaaactgta cagttttggc tgagcataat
     2161 gatgagtgat tttactgcat tgatataaaa agaaaaaatg atgccaaagc atcatttttc
     2221 ctatctcagt tggcatcaaa acgctaccca ctaatggagg gaaacctatt ttcttgaaag
     2281 gaaaattctc agcgcctgca tttttgcccc taaagtgtta ctcctgaagc agcgaaacaa
     2341 atcgaaccat tttcctctcc ccagatagtt taggttgaag agatcggaac gtttgtaata
     2401 gcacatgaca aaaatgccct ggtcatcgtc cacaatgtta ttgtttagtg ttattttttg
     2461 actttccaga actaatttat agaattcttt ccatttatgc tgtgaaccaa caattgcgcc
     2521 cccaataata tatacatgat tgccaatcat gaaatcaaaa gcctgttgtt gagatgtaac
     2581 agtaagccct ttttttatag tgaaaagatg catcttattt tcatcaaatg ggaaatccca
     2641 aattttcaat cctcgagtta catttggctt acggcaatag ccaaaatcta tccacgcaac
     2701 aagaggcgtt ttcactaaac ccatgttgat agctttattt acaaaatagg ccttaaggtt
     2761 acataccaaa acatactctg gtgaccagta ctctgggttt tttaattgtc gaggctcaag
     2821 tctatttgta aatgactcat ctttttgaat tttttcgata cgacttctga tatatctaaa
     2881 tttctttttt atatcgataa cgataaccgt tgttggtttt ccgttgcgaa tcgcttcaat
     2941 ccttggtttc aaatcgggag aagtgaaaat aatcatctca ttttcaagtg cggccagacg
     3001 ctcaaagtaa ctaaaataaa catcaactga acgagctagt ttttcacgga accctttatt
     3061 agcagtccaa tcccctctac caatgtcaaa atatgcagtg ataattgtgg tggatgattt
     3121 cattactaat aagaccatta atataaaaaa agaattatat cataaatatt aacagaatgt
     3181 atactgtata ttatccattc aaatgattcc gttatttatc atacatcgga atattgatac
     3241 taaagcacta ttcacatgca aaaccaacat ccgccatgaa ggactagcta aaacccaaac
     3301 tagtttgttg caattagcat ccttgcacct ctatgtaaag ggctgaaggg attcggatgt
     3361 gatggtatga ttacagacat tcgtgtctga gattgtctct gactccataa ttcgaaggtt
     3421 acagttatga tcatcgttac cggcggcgcg ggctttatcg gcagcaacat cgttaaggcc
     3481 ctgaatgata aaggcatcac cgatattctg gtggtggaca acctgaaaga cggcaccaag
     3541 tttgtgaacc tggtggatct ggatatcgcg gactatatgg ataaggaaga cttcctgatc
     3601 cagattatgg ctggcgaaga gttcggtgat gtcgaagcga ttttccacga aggcgcatgc
     3661 tcttccacca ccgagtggga tggcaagtat atgatggata acaactatca atactccaaa
     3721 gagctgctgc actactgtct ggagcgcgaa atcccgttcc tgtatgcctc ttccgcagcc
     3781 acctacggcg gacgcacctc cgactttatt gaatcccgcg agtacgaaaa accgttgaat
     3841 gtctacggtt actcaaaatt cctgtttgat gaatatgttc gtcaaatcct gccagaagcg
     3901 aactcgcaga ttgttggctt ccgctatttc aacgtttatg gaccgcgtga aggccataaa
     3961 ggcagcatgg cgagcgtcgc tttccatctc aacacccagc ttaataacgg tgaatcaccg
     4021 aagctgttcg aaggtagcga gaacttcaaa cgtgacttcg tctatgtggg cgacgtggca
     4081 gatgtaaacc tgtggttcct ggaaaatggc gtttccggta tcttcaacct cggcaccggt
     4141 cgtgcagaat ccttccaggc agtagcagat gctacgctgg cttatcacaa gaaaggccag
     4201 atcgaataca ttccgttccc ggataagctg aaaggccgct accaggcgtt cactcaggca
     4261 gatctgacaa atctgcgcgc ggcgggttac gacaaaccgt tcaaaaccgt tgctgaaggt
     4321 gtaacggaat acatggcctg gctgaatcgc gacgcataag agctctgcat gaaaatactg
     4381 gtgatcggcc cgtcttgggt tggcgacatg atgatgtcgc aaagtctcta tcgcacgctc
     4441 caggcgcgct atccccaggc gataatcgat gtgatggcac cggcatggtg ccgtccatta
     4501 ttatcgcgga tgccggaagt taacgaagct atccctatgc ctctcggtca cggagcgctg
     4561 gaaatcggcg aacgccgcaa actgggtcat agcctgcgtg aaaagcgcta cgaccgcgcc
     4621 tacgtcttac caaactcctt caaatctgca ttagtgcctt tcttcgcggg tattcctcat
     4681 cgcaccggct ggcgcggcga gatgcgctac ggtttactca acgatgtacg cgtgctcgat
     4741 aaagaagcct ggccgctaat ggtggaacgc tatgtcgcgc tggcctatga caaaggcatt
     4801 atgcgtaccg cacaagatct gccgcagcca ttgttatggc cgcagttgca ggtgagcgaa
     4861 ggtgaaaaat catatacctg taatcaattt tcgctttcat cagaacgtcc gatgattggc
     4921 ttttgcccgg gtgcggagtt tggtccggca aaacgctggc cacactacca ctatgcggaa
     4981 ctggcaaagc agctgattga tgaaggttat caggtggttc tgtttggctc tgcgaaagat
     5041 catgaagcgg gcaatgagat tcttgccgct ttgaataccg agcagcaggc atggtgtcgg
     5101 aacctggcgg gggaaacaca gcttgatcaa gcggttatct tgattgcagc ctgtaaagcc
     5161 attgtcacta acgattctgg cctgatgcac gttgcggcgg cgctcaatcg tccgctggtt
     5221 gccctgtatg gtccaagtag cccggacttc acaccgccgc tatcccataa agcgcgcgtg
     5281 atccgtctga ttaccggcta tcacaaagtg cgtaaaggtg acgctgcgga gggttatcac
     5341 cagagcttga tcgacattac tccccagcgc gtactggaag aactcaacgc gctattgtta
     5401 caagaggaag cctgacggat gcgggttttg atcgttaaaa catcgtcgat gggcgatgtt
     5461 ctccatacgt tgcccgcact cactgatgcc cagcaggcaa tcccagggat taagtttgac
     5521 tgggtggtgg aagaagggtt cgcacagatt ccttcctggc acgctgccgt tgagcgagtt
     5581 attcctgtgg caatacgtcg ctggcgtaaa gcctggttct cggcccccat aaaagctgaa
     5641 cgcaaagcgt ttcgtgaagc gctacaagca gagaactatg acgcagttat cgacgctcag
     5701 gggctggtaa aaagcgcggc actggtgaca cgtctggcgc atggcgtaaa gcatggattg
     5761 gactggcaaa ccgctcgcga acctttagcc agcctgtttt acaattgtaa gcatcatatt
     5821 gcaaaacagc agcacgccgt agaacgcacc cgcgaactgt ttgccaaaag tttgggctat
     5881 agcaaaccgc aaacccaggg cgattatgct atcgcacagc attttctgac gaacctgcct
     5941 acagatgctg gcgaatatgc cgtatttctt catgcgacga cccgtgatga taaacactgg
     6001 ccggaagaac actggcgaga attgattggt ttactggctg attcaggaat acggattaaa
     6061 cttccgtggg gcgcgccgca tgaggaagaa cgggcgaaac gactggcgga aggatttgct
     6121 tatgttgaag tattgccgaa gatgagtctg gaaggcgttg cccgcgtgct ggccggggct
     6181 aaatttgtag tgtcggtgga tacggggtta agccatttaa cggcggcact ggatagaccc
     6241 aatatcacgg tttatggacc aaccgatccg ggattaattg gtgggtatgg gaagaatcaa
     6301 gtagagtgtc gttctacaag tatgtctctt gcagatttgc cagctcaaac cgtttttcaa
     6361 aatttaaatc ttgaaataat aaccaataag ttgacatcgg agataagatg acctcaacat
     6421 tatttttctc tctcgagaaa aaaaactgga tagcgtactg gaacagagct ctcgtattct
     6481 tattcattac cacctatttt ttgggtggga ttacaaggta taaacatctt attgttattc
     6541 ttatgacaat aacgacaatc gtctatctct gcaaacggcc aaaacactat ctctcactat
     6601 ttaaaacatt tctttttggt agtgttgcca tattaactat tgctgcattg ctgtcacttc
     6661 ttcaatcccc tgatgcaggt gctagcatga aggaagtttt caaagctatt attgagaata
     6721 ctttactatg cacaatagca ataccggtca tattgagaga cgagaaaaga gaagatgtcg
     6781 aaaaaatcgt tttcttctca tttattagtg cgttgggctt acgctgtttt tctgaattga
     6841 ttacctatta taaggactat caacaaggga taatgccatt cgcagattat agacaccgta
     6901 gcatttctga ctcgatggtc tttttattcc ctgcattgtt aaatctctgg cttatcaaat
     6961 cagcaaaata ccgcatttct tttgtggttc taagcgttat ttttattttt ctgatattag
     7021 gaactttatc cagaggggcc tggctttccg tgttagtcat tggattaata tggattctga
     7081 tgtttaaaca atggaagtta ctattagtag gagtaatggt ttccatcatt gcattgtcgg
     7141 ttattttcac acataaggag atgaccgcaa agctaacgta taaacttcaa caaactaata
     7201 gttcttatcg ctatgcaaat ggtactcaag gcagcgcact cgatctaata ttagaaaatc
     7261 ctgttattgg ttatggttac ggtaacgttg catataaaga tgtctataat aaacgtgtca
     7321 ttgattatcc agaatggacc tttagacaat caatagggcc acataatttt gcgctattca
     7381 tctggtttgg cactggttta ttagggctgg taagtcttat gatgctatac tgtgcaatat
     7441 tgaaagagtg tataaaaaat ggcgtcaaga ataaatatcg ctcaccatat aatgcatatt
     7501 atataatctt actatctttt ataggttatt ttgttatccg tggaaacgta gaacaaattg
     7561 aaccaaattt attaggcgtt tacgccggct tattattagc gatgaaaaac aagtaaaaaa
     7621 taaaaaaggc tgcataatgc ggccttttta tttattcaaa ccaattatga ataacctctt
     7681 cgaatctttg agttacgcca tcccaactgt agtgatcaaa cacaaagtcc tgaccttgct
     7741 ttgcaactgc tgttaattca ggattcgcta acgtttttaa aatatcgctg cttatggagt
     7801 ctgcagtcat aggttctttt aaatggaatc ccgtagtatt ttctttgaca aattccgtca
     7861 tccccccacg cgtactcact aaaacaggct taccagcacc catagcttca attgccacca
     7921 tgcaaaatgg ttcctggaac tgggaaggaa tgacgactaa atctgccagt gggtaataac
     7981 aatacatctt ttcaggcggt atgcttccta acataatgca acgatccttc aaacgttttg
     8041 caatttccct gacattgcgt tgatatgcac ctttgtcact tttactcatt tctgtataat
     8101 caccaatcac aactaatttt aaatttttgt gagcagcggc caatttttcg aatgattgca
     8161 tcaacagcaa tatgcctttg tcagggacga tacgcccagc ataaaaaata attttctctt
     8221 caggagagat attaagttcg cttctagtga tcggctgaaa attagattga taggtttcta
     8281 aatcaattcc atttggtaca atctcaatat ctgcatttgc taaataagat tggtaatact
     8341 ttttcaagta taaactaggg actatcattt taacattttg ctcaagcaat tttggttcaa
     8401 atgcattatg catatgtata accactcttg cctgaggagc tagcttacga atttgagtgt
     8461 atagcttcat gctattatga actacaataa cactttcttt cgttatagga aaatcatgtg
     8521 caatattcaa tattctctgt gaatatggaa gaggatcaag tcttgtccat ttttgaaata
     8581 accgcttata tatgcgactg aatccaaccc tatgaatact acaattatca ttaacaaagg
     8641 tgtaatttga gtatcctgga tttttgatac aaacaatacg atttggaaaa ttggtacgct
     8701 gagcaacctg atacatccac gtttcaacag cagccgctcc gcgaggagga attgaaaaaa
     8761 taggagtaac cgtaaatatt attttatcaa ccatctcgta tattaacctt tcataacatt
     8821 atatttaatt gctgtaaaaa caccatcgag aaattttttc tggtaaagca agtgtttata
     8881 tttttctttg tgctcatgtt ttttaacagc ttttttataa ggtatatttc tccatggtga
     8941 tatattataa atattacgaa aataatctgc agaggcgtag ttcgcccaat catgccatgg
     9001 cttagttata ccagtataat gtatgaagac agtgtcatca ttaataaacc gggtgtaata
     9061 ttcactattt ttttcttcaa attctgactt tatagtataa atacaattat attttctcgg
     9121 taatatttta gcatgatgta gaaacattaa gtttaaaata tcttgatccg ggtactttaa
     9181 tttttttata attgactcat ctgataatag atcaaagaat ttttccgtta gtctttgttt
     9241 taaccattcc cgtaaattaa tatacattac accagagtta aaatagctac cgttaaaatc
     9301 ctcattacac aaacgctcag cgcttttact ttgcatagca tctacatcaa ttaccactgc
     9361 cccatattca tctttaaact ctaatgctat taactcgttc aatgaaccct tacagacgat
     9421 atcagcatca agatataata atgaatccaa tcgcgcagaa aaataatcaa atgaaagcaa
     9481 acgataataa atagatactg gccagatatt tgattgaggt agcgccttca atggctcaga
     9541 atcaatatga tataatttaa ttactgtttg atacttcgtt gctaattgtg ataataattc
     9601 aatatactct ttgtctaagt aatcagcgat tatgtgaaag gtaaatttgt tttgtttatt
     9661 cttttcaaga attgaagcaa ttgaaatcgc agccccaacc tggtagttct catcaatacc
     9721 ccatgagaca ttaaacgttt cccgttcatc aagtttaacg gggcgcttgt ccagttcaat
     9781 tatatcttta aattgagtaa gatgtttaaa atccaattta attacccttc atcataatca
     9841 ttatttgctc ttcaatccta gtttgacttt tacatccctg attacttttt taattttctt
     9901 tttgaatata agatatgtat agccaaagtc tttcaattca ttttttatat tatagtgccg
     9961 ttcaagatca atgcgatctt ttgcttttaa aacagcggtt gtttttttac ctgaaaggtc
    10021 aattagtctt aaaccttttt cggatactat aaaatttcct ttatgaggat cgccagacac
    10081 cattccatgc tgatgcaact cttttatcga ttctgataat tgatccttca gatcttcaga
    10141 aatttccaaa tactcattta acccgacacc ttcaatatac tcaatgagca taatataata
    10201 atgagcgaaa tttaatgttt tacgttcagc tagcaaaaaa tagtcattaa ttgattggat
    10261 gccttcacca cgtacccgat cggtttgata aattaaattt tcataataat ctttttttat
    10321 gcaagatttt aagaaacgtt cggtcatttt atgctttggc gcataaactt taagtacaag
    10381 aggtccacga gcagtgtcaa taagaatgac tttagtatca tcaatagaac gaaaaacttt
    10441 taatgtttta accctgcaag ttaaaaagtc atttagaact tgctcataaa agggatcatt
    10501 atcttttatg aaaactttca acccatttat cgttttatta tatatcataa tgaattattt
    10561 taaccttaaa tctttagaag catttttctt tataatactt taaataatta aaaatgcctg
    10621 ccatataatg cttttgttta aatttatgtt ttgcacaata ccgatactga ttgctattta
    10681 caggtttaag taaatcttct ttactccacg gagaagctgc tttggcgatc aaaaaactac
    10741 gtgaaaccgg ataatcagcc cactcatgcc agggttttgt aggtcccaca tagtgtataa
    10801 acacggtgtc atcattgact ggattatcaa ctttgtcttt taattcatag ttaatactat
    10861 atcgggtatt atatttctcc gaaataaact tcactttacc attcaataat acattcagta
    10921 catcttgatc aaggtgggtg attttactta cccaatctgg gtcacgcaac atttcaatag
    10981 cttttgacga aatgttattt agattccact catcaatatt tatcagtaaa aaaccagcat
    11041 tgaaatatcc agaagctaac tgtggtgtag ttaataccga ggctcgattt tgccaccatt
    11101 caatatctct ttcagctaca actgcggcaa tttcattagt agaaaattga taatctaaga
    11161 gttctttaat actacccttg caagcaatat ctgcatcaag atatagtatt ttttcatgtt
    11221 tatgatagaa ataatctgca attataaatc gaaaatatgt tgcgtaagtc cagtttttcg
    11281 tgcttggtaa tgacttcagc ttatcacaat tgataacata aatattaatt cttgaattat
    11341 attgttttgc taaatcagaa aaatataatt tgtctttatc actgatataa tctgtgaaaa
    11401 tatgaaattc acaagagatt tctctattgt ttaatagaat agatgcgatg gctacaccac
    11461 atccaaaaag aaaatttctg tcaataccaa atgctatatc aagagtattt ttagatctta
    11521 tattttgata atgaaaatta tactcaaaaa ggatgatgtc actatcattg agttgagaca
    11581 tatttattct gtatccaaaa ttttggcaaa aataagttac gggttacaac gattttcgaa
    11641 tcgttctttc cctgattttt gtggcttttg cttctgcttg cgacagcagt ccttgttcct
    11701 gcttgagaat gtctttaagc ggggcggcaa aatacacctt cataaagcgc cagatatccc
    11761 gctgagtcag gccaatattc attgaagaaa aataaagccc tataagatct ttatcgcgcc
    11821 aacgacctgg aacgcgcgtg cgaagctgcg cccggtgtag gtcaattacc gaaattttta
    11881 actcctcttc cttaccggaa aaaggcaagt gcagcaggaa atgacagata taacagtcac
    11941 gatggttaat gcccgcagca tgcatatcgc gcaccatcgt cgcgacacgc ttaataagca
    12001 tacgctttac gcgaacatct ggtgggttgg tcgcccagtc agcacagtaa tcttccagac
    12061 tgatggttgg tgtcagatct tcggtaataa taaacgaggt tctggtcagc ggattcatgc
    12121 ctttttcgcc gaatgccacc ccatacatag tatcaacgcc gacatcccgc agtcgatgaa
    12181 tcgcattcca ttcgcggtct gcgcctaata ctggcatccg caatgagagt aaatttttga
    12241 ttatctcttt cagggtcgtg ccgcgatgcc atttgagaaa atagcttttt cccgccattt
    12301 caaagcgcag agtacggcga gtttccagtt cacgaaatac ctcaccctgc aaggttttaa
    12361 cttcctcaaa aggatctttg ccgcgccata acgtggcaaa cggctcttta agttcaacca
    12421 tccagaccac ccgttatgat atccgccgct ttctctggca gactgtataa atcttgtgta
    12481 tcagcataat gtcgcgcatt ttccgcccaa gcctggcgca atgaagattg cgttaacgct
    12541 ttgcgtaaaa tctcattcaa tgtttcctgg cggaatggct cagcaatagc ctcgccgcaa
    12601 ttagcgtcga caatataatg cgcatagcca caaacggcag ttgttagtac cggtaatcct
    12661 gcagtaatcg cttccagcag cacaattccc gccgcttcct ggtaggcagg atgcagtaat
    12721 aaatccgccg ccgccattaa ttccgagaca tcgttgcgcc ccgagaagaa gtgaacatta
    12781 ctgcgcacgc cgcgtttttc tgccagtgcc tcaaattttc gcggtttatc ctgcccaaca
    12841 acatatagca atgtgttgtg gcgcagcgaa tccggtaacg aagcaagtgc ttcaatggaa
    12901 cgatcggcac ctttacgcgt gaagtctgaa ccgacctgca acaataaata ttgttgttcg
    12961 gttattccat tcttcttacg gaagatttca cggctattgg ctggctgctg gctatattta
    13021 cgatcaggat aaatccccgg tggcagaata tgaaaacgct ccgcttcagt ctgataatgt
    13081 ttctggaaat cggcgatttg cttatctgtc agcatcagca gctgtgtcgg cttgccctgt
    13141 tcgaaggttg cccgctcaaa ggcggcataa tggcgataac gtgacgtcag gcgatagaaa
    13201 aagccttttt cctgcgctac tttctcggca taacaaacat cagcggcata ataaacgtcc
    13261 agccccggca ttttgttgaa tccaacgact ttatcgacgg gatgttcgcg taaatgtttt
    13321 tgcacccagg caaaatactc cgcattgcgc ccgtgattgg tatgcgattt aaccggcact
    13381 ttgatcactt caaatacatc agggcattcg ccttcccacg actgggtata aacccgaaca
    13441 tgatgacctc gggcggcgac tgtctgagca atacgcataa aatcgcgctg caaaccgcca
    13501 aagggaaaat atttatataa acaaaaagca acgatcataa cgacgtacct gtcgtggagg
    13561 agggcagtaa tttatcgaca gcagcaatga catctgccgc cggaataacc gaaagataca
    13621 tctcatttcg gtcacgctga tcgcgcgttg gcatttcccg gtaatctccc gcccagaatt
    13681 gaatcatgtt atttgaccag ggacgccaga aaatatgatc tgttgcgcca aacagcgata
    13741 tcagcggcgt attaactgca gcggcaatat gcgccggtgc ggaatcaacg ccaataaaca
    13801 gctgcgcatg atcgattaac gcaccaagtt ccgggaaggt cacctttcca gccagcgccg
    13861 ttactggtgg cgtctggcat ccctgcgcaa tttcattgac gcaggccaga tcgtctttat
    13921 ctgggccgga cgtcagaacg acttcataac cacgagcatg taaggcatca atcacagcgg
    13981 aaaacttggc gttgtcccag catttgaaga tttgccgcgc cgtaggttgg ataaccacat
    14041 aattttgtcc aacaccagcg tgatcaagtt cgcgacgcat acgtttccag cttgcaggcg
    14101 ggtaactcat ggttgtctgc ttcaccaacg aatcaagtcc caatggtgtc agcacggata
    14161 agttactttc caccacattt ccaccctgca acggcaccaa atgggtgaaa cttttacgcc
    14221 aaaaagcaga ctgccgatga tgataatcct gggaaatttt cacacgagca tttaataagc
    14281 gaaccagtat agcaaccatc cattgatcgg taagattgac gataaggtca tacttattgg
    14341 cacgtaatac cttgatgaga tgaaaaaagt tggcaatttt ttctgaggct tttgcttttt
    14401 tattttttat gccgtagagc gcgttaatct ctggattttc agacaggatc gggatggtgt
    14461 cctgataaag cagcacatcg atttttgcgt cagggtaatt ttttttcaga gaactaatga
    14521 cgggagtagt taataacata tccccatgaa aacgcatctt aatgagcaaa attcttcgaa
    14581 atggctta
//
LOCUS       DAJTUP010000093        14500 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTUP010000093 DAJTUP010000000
VERSION     DAJTUP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.093"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..619)
                     /locus_tag="OO468_003049"
     CDS             complement(<1..619)
                     /locus_tag="OO468_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR6260079.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALE"
     gene            complement(818..902)
                     /locus_tag="OO468_003050"
     tRNA            complement(818..902)
                     /locus_tag="OO468_003050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(866..868),aa:Leu,seq:caa)
     gene            1098..2117
                     /gene="ahr"
                     /locus_tag="OO468_003051"
     CDS             1098..2117
                     /gene="ahr"
                     /locus_tag="OO468_003051"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR6260080.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2246..3748
                     /locus_tag="OO468_003052"
     CDS             2246..3748
                     /locus_tag="OO468_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR6260081.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(3867..4949)
                     /gene="lptG"
                     /locus_tag="OO468_003053"
     CDS             complement(3867..4949)
                     /gene="lptG"
                     /locus_tag="OO468_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR6260082.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(4949..6049)
                     /gene="lptF"
                     /locus_tag="OO468_003054"
     CDS             complement(4949..6049)
                     /gene="lptF"
                     /locus_tag="OO468_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR6260083.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6316..7827
                     /gene="pepA"
                     /locus_tag="OO468_003055"
     CDS             6316..7827
                     /gene="pepA"
                     /locus_tag="OO468_003055"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR6260084.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            7961..8404
                     /gene="holC"
                     /locus_tag="OO468_003056"
     CDS             7961..8404
                     /gene="holC"
                     /locus_tag="OO468_003056"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR6260085.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8404..11259
                     /gene="valS"
                     /locus_tag="OO468_003057"
     CDS             8404..11259
                     /gene="valS"
                     /locus_tag="OO468_003057"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR6260086.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11313..12509)
                     /locus_tag="OO468_003058"
     CDS             complement(11313..12509)
                     /locus_tag="OO468_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR6260087.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            12702..13205
                     /locus_tag="OO468_003059"
     CDS             12702..13205
                     /locus_tag="OO468_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6260088.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13251..13667)
                     /gene="rraB"
                     /locus_tag="OO468_003060"
     CDS             complement(13251..13667)
                     /gene="rraB"
                     /locus_tag="OO468_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR6260089.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            13829..>14500
                     /gene="argF"
                     /locus_tag="OO468_003061"
     CDS             13829..>14500
                     /gene="argF"
                     /locus_tag="OO468_003061"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR6260090.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVE"
BASE COUNT         3734 a         3691 c         3763 g         3312 t
ORIGIN      
        1 gttcaagagc tgcacggtgc tgtcttttcg ccgtagcaac cgcacctgca atctcttgcg
       61 cggggttgta gtcgattaag ccgctctgaa cggcaaagcg cataatcgcg gtagtacgct
      121 gctgtaaacg ggcggcaact tcgagccgcc cggatgactc gactgcttta atgggtacaa
      181 gaagatcccg cgtcttcagt tccgcaatgt tccgcttacc gatagcagta aagagattat
      241 cctccagact tttcaaaaca cgagcgctat gcgatgccga ccacttctga ttgctggcgt
      301 gccagtctct ggcgactact tcaaacgtta tcgcctcttg ttcctgttct accttaacag
      361 ctttcttgtt ttcactggga tcgacaccat tagctaaaag cttacgagcc tcatcccggc
      421 gtgccctggc atccgccaac gacacttcgg ggtatttccc caatgccagc atcttctctt
      481 tgccgccaaa gcgataacgt agccgccagt atttggagcc gttagggtga accagcaaaa
      541 ccatgccttc accgtcggtc agcttatagg cttttgcttc aggcttagcc gaacgaacct
      601 tcacatcact cagagccatg atgagtatcc tttcaagggt tctgtgtggg tacaaacatt
      661 atcgaaccgg gaaatacccg cagttgtacc cgcatcagta agttgatgta gattgaatca
      721 ggttgactta ggttgagtga aaaagcgagg aaagccttgc ggatactgga ttttaggcac
      781 aaaaaaagac gtccgttgac gtctattgat gttccgatgg tgccgaaggc cggactcgaa
      841 ccggcacgta tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg
      901 gcactgaaga ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag
      961 cgccagcttg cacccacctc gctaagtgct gaaattttca gcattacact cagcgatatg
     1021 taacctttgt cacactccag gcaccccgcc ctgccatgct ctacacttcc caaacaacac
     1081 cagagaaggc cccaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg
     1141 aactggaagt ttatgagtac gatcccggtg agctgaagcc acaagatgtt gaagtgcagg
     1201 tggattactg cgggatttgt cattccgatc tgtcgatgat cgataacgaa tggggatttt
     1261 cacaatatcc gctggttgcc gggcatgagg tgattgggcg cgtagtggca ctcgggagcg
     1321 ccgcgcagga taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta
     1381 gctgtggtca ctgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg
     1441 tgccgacaat tatgaatcgc ggtggctttg ccgagaagtt gcgtgcggac tggcaatggg
     1501 tgattccact gccagaaaat attgatatcg agtccgccgg gccgctgttg tgcggcggta
     1561 tcacggtctt taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa
     1621 ttggtattgg cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg
     1681 tgacggcctt tagttctaat ccggcgaaag agcaggaagt actggcgatg ggtgccgata
     1741 aagtggtgaa tagccgcgat ccgcaggcac tgaaagccct gtcggggcag tttgatctca
     1801 ttatcaatac tgtgaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacctatg
     1861 gcggtaattt ccatacggtc ggtgcggttc tcacgccgct gtctgttccg gcctttacgt
     1921 taattgcggg cgaccgcagc atctctggtt ctgctaccgg cacgccttat gagctgcgaa
     1981 agctgatgcg ctttgccgcc cgcagcaagg ttgcgccgac aaccgaactg ttcccgatgt
     2041 cgaaaattaa cgacgccatt cagcatgtac gcgatggtaa agcgcgctac cgagtagtcc
     2101 tgaaagccga cttctgatcc ctttgccggg tgctgtcgcc cggcacttaa atctctttgg
     2161 aaggcgtctc gcaatctgct gaatttagcg gtgaagagtg cacagccgtt gcctatactc
     2221 caggcaggaa actggaggaa atctcatgag cgaacccctg ttaattgccc gcacgccgga
     2281 cacagaactg tttttactgc cgggaatggc taaccgtcac gggctgatta ctggcgcaac
     2341 ggggacgggt aaaaccgtta cgctgcaaaa actggcagag tcattgtcgg aaatcggcgt
     2401 gccggtgttt atggctgatg tgaaaggcga tctgaccggt atcgcgcagg caggaacggc
     2461 gtcggaaaaa ctgctcacaa ggcttaaaaa tatcggcgtc aatgactggc aaccgcatgc
     2521 caatccggtg gtggtgtggg atatctttgg cgagaaaggc cacacggtgc gggcgacggt
     2581 ttcggatctg gggccgctgt tgctggcgcg actgttgaat ctcaacgatg tgcaatctgg
     2641 cgtgctgaat attatcttcc gcattgcgga cgatcaggga ttgttgctgc tcgactttaa
     2701 agatttgcgg gcgattaccc agtacatcgg cgataacgcc aaatccttcc agaatcagta
     2761 cggaaatatc agtagcgcat cggttggtgc catccagcgc ggattactgt cgctggaaca
     2821 gcaaggcgca gcacacttct ttggtgagcc gatgctggat atcaaagact ggatgcgcac
     2881 cgatgccaac ggtaaaggcg ttatcaatat cctcagcgcc gagaaacttt atcagatgcc
     2941 gaaactgtac gccgccagcc tgctgtggat gctttcagag ttgtatgaac aattgccgga
     3001 agcgggcgat ctggagaaac caaaactggt gtttttcttc gacgaggcac atctgctgtt
     3061 taacgatgca ccgcaggtac tgctggataa gattgagcag gtgataaggc ttattcgctc
     3121 aaaaggcgtg ggcgtctggt tcgtttcgca aaacccgtct gatattccgg ataatgtgtt
     3181 cggccagctc ggtaatcgtg ttcagcacgc tttgcgggct tttacgccaa aagatcagaa
     3241 agcagtgaag acagcggcgc aaaccatgcg ggccaatccg gcatttgata ccgaaaaggc
     3301 aatccaggaa ctggggaccg gcgaggcgtt aatctcgttt ctcgatgcaa aaggaagtcc
     3361 ttctgtggtg gaacgggcga tggtgatcgc accttgttcg cgaatggggc cggtgacgga
     3421 agatgagcgt aatggcctga ttaatcactc cccggtgtat ggcaaatatg aagatgaggt
     3481 ggaccgcgag tccgcctatg agatgctgca aaaaggcttt caggccagta ccgagcagca
     3541 aaataatccc cccgtgaaag ggaaagaggt ggcggtggat gacggtattc ttggtggatt
     3601 gaaggatatt ttgtttggca ctaccggacc acgcggcggg aagaaagatg gtgtggtgca
     3661 aacaatggcg aaaagcgccg ctcgccaggt gacgaatcag attgtacgcg ggatgttggg
     3721 gagtttgctg ggggggagaa aaagataagt cagacgtgat ctgtgcccct caccctaacc
     3781 ctctccccaa aggggcgagg ggaccgttcg aactcgtatt tgactttgtc aacaataata
     3841 aagggagctt tcgctccctt gttagattac gattttctca ttaacagcca caggctgatt
     3901 aagaagaagc tggcacttgg caacagtgcg ccgatgatcg gcgggatgcc gtaaaccaac
     3961 gtcagcgggc cgaagatctg gtccagtacg tagaagacaa aaccgaagct gatacctgtg
     4021 accacacgca cgcccatcgg tacgctacgc agtgggccaa agataaacga cagcgccatc
     4081 agcatcatca ccgccacgga tagcggctgg aagattttgc tccacatgtt gagctgataa
     4141 cgtccggcat cctgaccgct cgacttcaga tacttcacgt agttgtgcaa accactgatg
     4201 gagagtgcat ccgggtccag cgccaccacg cccagtttgt ctggcgtgag gtcggttttc
     4261 caggtgccgc tcaccgtctg cgaaccggta atctgtttcg gattggtcag atcagattca
     4321 tcaacctgcg acagacgcca gactttatgt tccgggtcaa acttcgcggt agcggcataa
     4381 cgtacggatt gcagacgacg attctcgtta aaggcataaa tactgatgcc acctaacacc
     4441 tcgtcacctt taacccgctc aatgtagacg aagttgttgc catctttcgc ccataaacct
     4501 tgctgggtag agagcaacga gccgccgtac atcgcctgcg cacggtagtt acgcgccatc
     4561 tgttcgccct gcggcgcgac ccattcgcca atcgccatcg tcagcaagac cagtggaatg
     4621 gcggttttca tcaccgacag cgctacctgc atacgggtaa aaccagaagc ctgcatcacc
     4681 accagttcgc tgcgctgcgc cagcatccca agaccaagca acgccccaag aagagccgcc
     4741 atcgggaaga agatctgcac atctttcggc acgctcagca aggtatacat tcctgcgcct
     4801 aacgcgtcgt aactcccctg cccggctttt ttcagctgat cgacaaattt gataatgccc
     4861 gacagcgaca ccagcatgaa cagcgtcatc atgatggtgg tgaaaatagt tttaccgata
     4921 tagcggtcaa gtacgccaaa aggttgcatc acaccgctcc tttacgcgaa aaactggcgc
     4981 gcaggcggcg gaccggcacg gtgtcccaaa ggttgagaac aatcgccaaa gccagataaa
     5041 tcaggttaac ggtccacatc cacagtgtcg ggtccagctt acctttaccg ccgttcgatt
     5101 tcagggaggt ctggatcagg aagaaaagta gatacagcag catggctggc agcatcgaca
     5161 gtacgcgtcc ctgacgcggg ttaaccacgc tcaacggtac aaccataagt gccatcataa
     5221 acacggtgaa taccaacgtg atacgccagt tcagttctgc gcgagcacga tcggtgtcag
     5281 tgttccacaa tgtgcgcatg tccatctggt cggtatcgtt cgggtcgagc gccaccgcct
     5341 ggtgaccaat gatcgcctga taatcctgga aatccgtaat gcggaaatca cgtaacagtg
     5401 cagtgccttc gaagcgcgtt ccctggttga gagtgacgac ctgggagccg tcgcgcagct
     5461 gggttaaatg tccggaatcg gccaccacca cagaaggacg tgcattacct tttggtcgaa
     5521 tttgtgcgag gaacacatct ttgaaatcgc tgccgtcaac gctttcgatg aacagcaccg
     5581 agctgccatt agtcgcttgc tggaattgcc cttgcgccag cgccgccatg ccagggttcg
     5641 ctttcgcttc tgctaacact tcatcctgat gacgcgatga ccacggtccc gcccacatca
     5701 cgttaaccgc cgcgacgatt gccgtgaata ccgcaaggat cattgccgct ttcaccagaa
     5761 ccgctttgct caagccgcag gcatgcatta ccgtaatttc actttcggta tacagtttgc
     5821 ccagcgtcat cagcagcccg aggaacaggc ttaatggcag gataagctgc gccatttccg
     5881 gcacgcccaa cccgagaagg gagagcacca gattcgccgg aatatcgccg tcaaccgctg
     5941 cgccgaggat cctcactaac ttttgacaga agaagatcaa aagcaagatg aagagtatcg
     6001 ccagctggct tttgagcgtc tcccgcacca gatatcttat gattatcact ttaaatacgc
     6061 ccgtaaaaac tcgtcttttg caggatttta gcttgtttca tggcttaaac gtcatttatt
     6121 ctcttgagtc gtcgaaatcg tcgctaagat aattatactc aacggattca cctctcagat
     6181 tttgttctga cgtgccaatg ccgtaataac gttaagatta acacgaagtc atcgcaacag
     6241 cggacatgag ttacgaaagc ttgcaattct atctgtagcc accgccgttg tctttaagat
     6301 tcaggagcgt agtgcatgga gtttagtgta aaaagcggta gcccggagaa acagcggagt
     6361 gcctgcatcg tcgtgggcgt cttcgaacca cgtcgccttt ctccgattgc agaacagctc
     6421 gataaaatca gcgatgggta catcagcgcc ctgctacgcc ggggcgaact ggaaggaaaa
     6481 ccggggcaga cattgttgct gcaccatgtt ccgaatgtac tttccgagcg aattctcctt
     6541 attggttgcg gcaaagaacg tgagctggat gagcgtcagt acaagcaggt tattcagaaa
     6601 accattaata cgctgaatga tactggctcg atggaagcgg tctgctttct gactgaactg
     6661 cacgttaaag gccgtaacaa ctactggaaa gtgcgtcagg ctgtcgagac agcaaaagag
     6721 acgctctaca gtttcgatca gttgaaaacg aataagagcg aaccgcgtcg tccgctgcgt
     6781 aaaatggtgt tcaacgtgcc gacccgccgt gaactgacca gcggtgagcg cgcgatccag
     6841 cacggtcagg cgattgccgc cgggataaaa gcagcgaaag atctcggtaa tatgccgccg
     6901 aatatctgta acgccgctta tctcgcctct caggcgcgcc agctggctga cagctacagc
     6961 aagaatgtca tcacccgcgt tatcggcgaa cagcagatga aagagctggg aatgcactct
     7021 tatctggcgg tcggtcaggg ttcacaaaac gaatcgctga tgtcggtgat tgagtacaaa
     7081 ggcaacgcgt cggaagatgc acgcccaatc gtgctggtgg gtaaaggttt aaccttcgac
     7141 tccggcggta tctccatcaa gccttcagaa ggcatggatg agatgaagta cgatatgtgc
     7201 ggcgcggcgg cagtttacgg cgtgatgcgt atggtcgcgg agctgcaact gccgattaac
     7261 gttattggcg tgctggcagg ctgtgaaaac atgcctggcg ggcgtgccta tcgtccgggc
     7321 gatgtgttaa ccaccatgtc cggtcaaacc gttgaagtgc tgaacaccga tgccgaaggc
     7381 cgcctggtac tgtgcgacgt gttaacttac gttgaacgtt ttgagccgga agcagtgatt
     7441 gacgtggcga cgctgaccgg tgcctgcgtg atcgcgctgg gtcatcatat tactggtctg
     7501 atgacgaacc ataatccgct ggcccatgaa ctgattgccg cgtctgaaca atccggtgac
     7561 cgcgcatggc gcttaccgct gggtgacgag tatcaggaac agctggagtc caattttgcc
     7621 gatatggcga acattggcgg tcgtcctggt ggggcgatta ccgcaggttg cttcctgtca
     7681 cgctttaccc gtaagtataa ctgggcgcac ctggatattg caggaaccgc ctggcgttct
     7741 ggtaaagcaa aaggcgcaac cggtcgtccg gtagcgttgc tggcacagtt cctgttaaac
     7801 cgcgctgggt ttaacggcga agagtaagtt tgcgttttac cctcacccta accctctccc
     7861 taagggagag ggaaccgatc gagcgcaata gaaattcatt gctcatcttt cagggtgagg
     7921 aatgcagcaa tatttaaatc caccacaaga agccccatat atgaaaaacg caacgttcta
     7981 ccttctggac aatgacacca ccgtcgatgg cttaagcgcc gttgagcaac tggtgtgtga
     8041 aattgccgca gaacgttggc gcagcggtaa gcgcgtactc atcgcctgtg aagatgaaaa
     8101 gcaggcttac cggctggatg aagccctgtg ggcgcgtccg gcagaaagct ttgttccgca
     8161 taatttagcg ggagaaggac cgcgcggcag tgcgccggtg gagatcgcct ggccgcaaaa
     8221 acgcagcagc agcccgcgcg atatattgat tagtctgcga acaagctttg cagattttgc
     8281 caccgctttc acagaagtgg tagacttcgt tccttatgaa gattctctga aacaactggc
     8341 gcgcgaacgc tataaagcct accgcgtggc tggtttcaac ctgaatacgg caacctggaa
     8401 ataatggaaa agacatacaa cccacaagat atcgaacagc cgctttacga gcactgggaa
     8461 aagcagggct actttaagcc taatggcgat gaaagccagg aaagtttctg catcatgatc
     8521 ccgccgccga acgtcaccgg cagtttgcat atgggtcacg ccttccagca aaccatcatg
     8581 gataccatga tccgctatca gcgcatgcag ggcaaaaaca ccctgtggca ggtcggtact
     8641 gaccacgccg ggatcgctac ccagatggtc gttgagcgca agattgccgc agaagaaggt
     8701 aaaacccgtc acgactacgg ccgcgaagct ttcatcgaca aaatctggga atggaaagcg
     8761 gaatctggcg gcaccattac ccgtcagatg cgccgtctcg gcaactccgt cgactgggag
     8821 cgtgaacgct tcaccatgga cgaaggcctg tccaatgcgg tgaaagaagt tttcgttcgt
     8881 ctgtataaag aagacctgat ttaccgtggc aagcgcctgg taaactggga cccgaaactg
     8941 cgtaccgcta tttctgacct ggaagtggaa aaccgcgaat cgaaaggttc gatgtggcac
     9001 atccgctatc cgctggctga cggcgcgaaa accgcagacg gtaaagatta tctggtggtt
     9061 gcgaccactc gcccggaaac cctgctgggc gataccggtg tagctgttaa cccggaagat
     9121 ccgcgttaca aagatctgat tggcaaatat gtcattctgc cgctggttaa ccgtcgtatt
     9181 ccgatcgttg gcgacgaaca cgccgacatg gaaaaaggca ccggctgcgt gaagatcaca
     9241 ccggcgcacg actttaacga ctatgaagtc ggtaaacgtc acgccctgcc gatgatcaac
     9301 atcctgacct ttgacggtga tatccgtgaa agcgcccagg tgttcgatac caaaggtaac
     9361 gaatctgacg tttattccag cgagatcccg gcagagttcc agaaactcga gcgttttgct
     9421 gcacgtaaag cagtcgttgc tgcggttgat gcactcggcc tgctggaaga aattaaaccg
     9481 cacgacctga ccgttcctta cggcgaccgt ggcggcgtgg ttatcgaacc gatgctgacc
     9541 gaccagtggt acgtgcgtgc cgatgtactg gcgaaaccgg cagttgaagc ggttgagaac
     9601 ggcgacattc agttcgtacc gaagcagtac gaaaacatgt acttctcctg gatgcgcgat
     9661 attcaggact ggtgtatctc tcgtcagctg tggtggggtc accgtatccc ggcatggtat
     9721 gacgaagcgg gtaatgttta tgttggccgc aacgaagaag aagtgcgcaa ggaaaataac
     9781 ctcggtgcca acgttgccct gcgtcaggac gaagacgttc tcgacacctg gttctcttct
     9841 gcactgtgga ccttctctac ccttggctgg ccggaaaata ccgacgccct gcgtcagttc
     9901 cacccaacca gcgtgatggt atctggtttc gacatcatct tcttctggat tgcccgcatg
     9961 atcatgatga ccatgcactt catcaaagat gaaaatggca aaccgcaggt gccgttccac
    10021 actgtttaca tgaccggtct gattcgtgac gacgaaggtc agaagatgtc caaatccaag
    10081 ggtaacgtta ttgacccgct ggatatggtt gacggtattt cgctgccaga actgctggaa
    10141 aaacgcaccg gcaatatgat gcagccgcag ctggcggaca aaatccgtaa gcgcaccgag
    10201 aaacagttcc cgaacggcat tgagccgcac ggcaccgatg ccctgcgctt caccctggcg
    10261 gcgctggcgt ctaccggtcg tgacatcaac tgggatatga agcgtctgga aggctaccgt
    10321 aacttctgta acaagctgtg gaacgccagc cgctttgtgc tgatgaacac agaaggtcag
    10381 gattgcggct tcaacggcgg cgaaatgacg ctatcgctgg cggaccgctg gattctggcg
    10441 gagttcaacc agactatcaa agcgtaccgc gaagcgctgg acagcttccg cttcgatatc
    10501 gccgcaggca ttttgtatga gttcacctgg aaccagttct gcgactggta tctggagctg
    10561 accaagccgg taatgaacgg tggaacagaa gccgaactgc gcggtactcg ccatacgctg
    10621 gtgactgtac tggaaggtct gctgcgcctc gcgcatccga tcattccgtt catcaccgaa
    10681 accatctggc agcgtgtgaa agtactttgc ggtatcactg ccgacaccat catgctgcag
    10741 ccgttcccgc agtatgatgc atctcaggtt gatgaagccg cactggccga caccgagtgg
    10801 ctgaaacagg cgatcgttgc tgtacgtaac atccgtgcag aaatgaacat cgcgccgggc
    10861 aaaccgctgg agctgctgct gcgtggttgc agcgcggatg cagaacgtcg cgtaaatgaa
    10921 aaccgtggct tcctgcaaac gctggcgcgt ctggaaagca tcaccgtgct gcctgccgat
    10981 gacaaaggtc cggtttccgt taccaagatc atcgacggcg cagagctgct gatcccgatg
    11041 gctggcctca tcaacaaaga agatgagctg gcgcgcctgg cgaaagaagt ggcgaagatc
    11101 gaaggtgaaa tcagccgcat cgagaacaag ctggcaaatg aaggctttgt cgcccgcgca
    11161 ccggaagcgg ttatcgcgaa agagcgtgag aagctggaag gttatgcaga agcgaaagcc
    11221 aagctgattg aacagcaggc tgttatcgcc gcactgtaat caacacagtg atgaaaacga
    11281 aggccggagc atgctccggc cttttttatc tcttacagaa atggtaaaga aggcataact
    11341 ccccgagaaa tactggctaa gaagccttta tctggttgtt cttctttatt gattaaagga
    11401 aggtcatcca aatcgcccag caaatacgta ttttttgcct gccagtcaat catccatatt
    11461 ttcagcagtg ggtaagccag accacccgta atcccggata tcaccaccaa cgcacacatg
    11521 cgataaagca taccgtggta cgttaaagtt gagcgaaaac gaatacgccc atcattcagt
    11581 gacaggttgc tcataaaatg atttcgcaac gacaccgtta aataactggt gctaacagca
    11641 atcccaaaat aataaattaa ctgcgcgatt atcattttac gttgcatttc cataaattgc
    11701 tgtaaattct caatatcatc atttgcatac ccagaactat catactcatt taatatttga
    11761 tcgaagataa tataaccagc aacagcgaga aaaggtaata aagctaaaaa tgcgaggatg
    11821 gcatatttta tacaatacgc agtatctaat ttcaccttga aacgatgtat accgaaactg
    11881 gtattgctcc agagaaaact cattaccagg ctatataaag taccattaaa aataccaatg
    11941 gaaacaatac caactattgc catcagaacc acagatataa taacactact tgaactattg
    12001 gcatgtagca tctttgtcga gataaagaaa acagtcccca tcccaatggc cattaaaatc
    12061 ggcaagaaaa aggttaccca ccagaaccct ttcaaagagc aattaaaact aaatcttacg
    12121 ccgttgagac tggtcattaa ggcctgataa cgcagtcctt ttgctgccat aaatataagc
    12181 aaaaccaaca gtaacaaagt caaaacacag ccaacaagcg gcatatctgc tgacactgtc
    12241 attaagattg cgaaatagaa aaaaacaaaa acaagacaac taacaaaaac attcccaccg
    12301 gtaattccat aagaaaatcg ttgtccgtta acttccatat tagcataaag ataacgctta
    12361 catttcatta atgcccatgg taaatagata cctagcgtta taatcgttaa caacacattc
    12421 accacacaaa taagaaaata tctctcacct gtaccatgaa aaacaaaaga atgggacgga
    12481 acatccattt cattaataac ttgagccata aaaatcccct gaattaaaaa atccattgat
    12541 ataatttata gatacagaca gtaaaatgtt ctgccacgtt acaatactct tatcagtaat
    12601 aggctggcaa aaactttatt gctttataag acgcttgcgc acgtaagaag agagtggtat
    12661 taaacaaatg tataattact ttatttttat tgggttaaac tatgaataac attgcgccgc
    12721 aatcacctgt aatgcgtcgc ctgacgctgc aggataatcc tgctatcgcc agcgtcatcc
    12781 gtcaggttta cgcagagtat ggtcttaccg ccgataaagg ctacaccgtt gccgatccga
    12841 atctcgacga actgtatcaa gtgtacagcc agcctggcca tgcttattgg gtggttgaat
    12901 acaatggcga agtggtcggc ggcggcggga ttgcgccatt aacaggtagt gaagcggaca
    12961 tttgcgaact gcagaaaatg tactttcttc ccgctattcg cggtaaaggg ctagcgaaaa
    13021 aactggcctt aatggcgatg gagcaggcac gagagatggg cttcaaacgc tgctatctgg
    13081 aaacgaccgc ttttttaaag gaagccattg cgctttatga gcatttgggc tttgagcata
    13141 tcgactatgc gcttggctgc acgggccatg tcgattgtga agtgcggatg ctacgcgaac
    13201 tctaaaaaaa tgcccgctag tatgtgctat cgggcatttt aatcaattaa ttagtggcga
    13261 accccgtcat cgtcttcatc gacaaaatct tcatcgtcgc catcttcgcc gttaggatct
    13321 tcaaagtaag tgccccaacc gtcgtactca acgtcaaatt tctccgccag cgtcatcagt
    13381 tgttcaacct gggcatcgat cagatcggca ttcaacgcgc actcgctgag gatgtcgcag
    13441 caaatcacga tatcaccatc ttcaacttcc agctcttctg gatcggtcac ttcgtaaccg
    13501 agtttaaacg cttcaactgc cgctttttcc agagtttcca gatcgtctgc ggaaagatgg
    13561 tgttcgatgg tgtacagcgc gtccggatcg ctgccatctt ccagtaattc ttcaataatc
    13621 aaacgtgttt cttcacgctg ttcttccagt tgttccgggt ttgccatggc tcattcctct
    13681 taatgtcctg ccgatactct tattgtcaca caccactgac attgcctcca cctttgtgac
    13741 aaagatttat gctttagact tgcaaatgaa taataataca cataaattga gttttaattc
    13801 attgaggcgt tagccacagg agggatctat gtccgggttt tatcataagc atttcctgaa
    13861 attactcgat ttcacgccag ctgaactcaa cagcctgctg cagttagccg cgaagctgaa
    13921 agctgataag aaaagcggta aagaagaagc caaactcact ggtaaaaaca tcgcgctcat
    13981 cttcgaaaaa gactcgactc gtacccgatg ctctttcgaa gttgccgcat atgaccaggg
    14041 ggctcgcgtt acttatcttg gcccaagcgg tagtcagatt ggtcataaag agtcgattaa
    14101 agacactgcc cgcgtgcttg gtcgcatgta tgacggtatt cagtatcgcg gctatggtca
    14161 ggagattgtc gaaacactgg cggaatacgc tggcgtgccg gtatggaacg gcctgaccaa
    14221 tgagtttcac cccacgcagt tgctggcgga tctcctcacc atgcaggagc atttacccgg
    14281 taaagcattc aacgaaatga cgctggtcta tgcaggtgac gcacgtaaca acatgggcaa
    14341 ttcgatgctc gaagctgcgg cgcttaccgg tctggatttg cgtctggtcg cgccacaggc
    14401 atgctggcca gaagctgcgc tggttacgga atgccgcgcc ctggcacaac aaaatggcgg
    14461 gaatattacg ctgacggaag atgtagctaa aggagttgaa
//
LOCUS       DAJTUP010000094        14470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTUP010000094 DAJTUP010000000
VERSION     DAJTUP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.094"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(114..380)
                     /locus_tag="OO468_003062"
     CDS             complement(114..380)
                     /locus_tag="OO468_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR6260091.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            654..4178
                     /gene="nifJ"
                     /locus_tag="OO468_003063"
     CDS             654..4178
                     /gene="nifJ"
                     /locus_tag="OO468_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR6260092.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4545..5674
                     /gene="ompN"
                     /locus_tag="OO468_003064"
                     /pseudo
     CDS             4545..5674
                     /gene="ompN"
                     /locus_tag="OO468_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5815..6249
                     /gene="uspF"
                     /locus_tag="OO468_003065"
     CDS             5815..6249
                     /gene="uspF"
                     /locus_tag="OO468_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR6260093.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6425..7360
                     /gene="ttcA"
                     /locus_tag="OO468_003066"
     CDS             6425..7360
                     /gene="ttcA"
                     /locus_tag="OO468_003066"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR6260094.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO468_003067"
     CDS             complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO468_003067"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR6260095.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO468_003068"
     CDS             complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO468_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR6260096.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLYRSKWL"
     gene            10572..11804
                     /gene="dgcM"
                     /locus_tag="OO468_003069"
                     /pseudo
     CDS             10572..11804
                     /gene="dgcM"
                     /locus_tag="OO468_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO468_003070"
                     /pseudo
     CDS             complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO468_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12642..14096)
                     /locus_tag="OO468_003071"
     CDS             complement(12642..14096)
                     /locus_tag="OO468_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR6260097.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14093..>14470)
                     /locus_tag="OO468_003072"
     CDS             complement(14093..>14470)
                     /locus_tag="OO468_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6260098.1"
                     /translation="DGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGM
                     KPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIAF
                     DDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3859 a         3648 c         3414 g         3549 t
ORIGIN      
        1 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
       61 acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
      121 ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca
      181 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
      241 gcacaatttg cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc
      301 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
      361 acccaaaacg ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat
      421 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
      481 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt
      541 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac
      601 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
      661 ctattgacgg taatggcgcg gttgcttcgg tgacgtttcg catcagtgaa gttatcgcca
      721 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
      781 gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
      841 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
      901 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat
      961 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc
     1021 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc
     1081 aggaagcgca agactttgct ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat
     1141 ttattcattt ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg
     1201 ccgatgacac tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg
     1261 cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     1321 agtctcgcga agccaccaat ccctggtaca acgcggtcta tgaccatgtt gaacaggcga
     1381 tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc
     1441 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     1501 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc
     1561 ccttctccgc caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     1621 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg
     1681 cactggcaga agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc
     1741 gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc
     1801 tcaacgcggc taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc
     1861 tgtcactgcc gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt
     1921 atggccttgg tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta
     1981 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc
     2041 tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     2101 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     2161 gtttaaaacc tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt
     2221 cgcgcttgcc gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     2281 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     2341 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     2401 agggtgcgat tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc
     2461 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc
     2521 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     2581 ccgcggcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     2641 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     2701 tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag
     2761 ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     2821 cgctagatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     2881 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     2941 cagagattaa agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca
     3001 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     3061 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg
     3121 gcgagacgcc gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta
     3181 acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     3241 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     3301 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     3361 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag
     3421 aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac
     3481 atgaacttct gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg
     3541 acggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     3601 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     3661 aagcgacacc gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta
     3721 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     3781 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat
     3841 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg
     3901 accagatgcg ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc
     3961 gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg
     4021 aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg
     4081 cagaacagtt atggaaagat gctgcagcta atttgcaaaa acgctatgac ttcctagcac
     4141 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     4201 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     4261 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
     4321 cgcagaataa cgtataggaa attaaaagta taatttttat tttgtatgta ttatcttcga
     4381 tacattcacc agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg
     4441 cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt tcactccccg
     4501 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agaaaagtac
     4561 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     4621 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
     4681 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     4741 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
     4801 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
     4861 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     4921 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
     4981 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
     5041 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
     5101 ccaataacga acgcgatgtt cgtcatgaaa acggtgacgg ctggggtctt tccacaacat
     5161 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
     5221 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
     5281 aatacgatgc taacaatatt tacctagcaa ccatgtattc agaaacgcgt aatatgaccc
     5341 cgtttggcga cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactagta
     5401 ccagtttgat ttttgtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct
     5461 tcacgctgct ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata
     5521 cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacctatg ttgactataa
     5581 aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga
     5641 tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg
     5701 ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca
     5761 caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac
     5821 agaacgattc ttgtccctat cgatatttca gattcagaat taactcaacg cgtgattagc
     5881 cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct
     5941 tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat
     6001 gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca
     6061 accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg
     6121 gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact
     6181 tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt
     6241 gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg
     6301 ctgacatttt cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta
     6361 tgctgagcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
     6421 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca
     6481 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
     6541 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
     6601 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
     6661 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
     6721 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa
     6781 aaccacttgc tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga
     6841 actggggacg acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt
     6901 cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
     6961 tggcaaacat atcgttattc gtccactggc ctactgccgc gagaaagata ttcagcgatt
     7021 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     7081 gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
     7141 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     7201 gttcgatttc aaaggcatta cccacggttc tgaagtggtt aacgggggtg atctggcgtt
     7261 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
     7321 gttggatgag ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatgcg
     7381 caagcgtatc gggctactct tatggaggcc ggataagacg cggccagcgt cgcatctggc
     7441 aatcccgaat aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa
     7501 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
     7561 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
     7621 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
     7681 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat
     7741 ggaactatta gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
     7801 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
     7861 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
     7921 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcaaccag
     7981 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgttgct ccagatcgcc
     8041 gtgtaacgac aatgcacttt gctctacttc attcagcgcg tcgcagacag cctggcaatc
     8101 ttttttggta ttgcaaaaca ccacacaaga ggatggctga tgcaagctta ataaccgttg
     8161 caacagagga attttgcctt tactggatgt ctcataaaat tgttgttcaa tgggtggcaa
     8221 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc
     8281 ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     8341 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     8401 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     8461 acgacccggc gttgccacga taatatgcgg cgcatgttgc agcgaatcac gctgcatacc
     8521 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     8581 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     8641 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     8701 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     8761 ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc
     8821 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     8881 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     8941 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     9001 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     9061 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     9121 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     9181 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     9241 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     9301 tttgtataca gacagccgga aaaattgctt ttgttacaac catttactac ggtacaacca
     9361 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     9421 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     9481 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     9541 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     9601 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     9661 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     9721 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     9781 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     9841 actggaatga tcggtcaacg catcgcacac atccaccagc catcccccgc aatcggtcgg
     9901 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     9961 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
    10021 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgaaacggct
    10081 gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
    10141 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
    10201 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
    10261 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccactgt
    10321 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
    10381 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
    10441 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
    10501 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
    10561 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
    10621 ggattgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
    10681 tgcaagacct cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag
    10741 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
    10801 ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag
    10861 aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag
    10921 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
    10981 ctaactctgc accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta
    11041 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
    11101 aggaaataaa tatgcttggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
    11161 ccggcggtca taaccctttg aattttgttc ataaactggc ggatggatct actcgtcatg
    11221 tgcagaccta tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc
    11281 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg
    11341 cgatgaccgg attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc
    11401 agcatctcgc catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac
    11461 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgcc
    11521 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
    11581 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
    11641 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
    11701 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
    11761 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
    11821 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
    11881 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg
    11941 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
    12001 gtgatttatc ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca
    12061 gggcttgttg aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa
    12121 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgct
    12181 acaacccttc ctgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg
    12241 tagtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
    12301 ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
    12361 gaaacagcga tttgtcgtca aggttcatgt aacctcctat gcaccttcac acgtcgtgat
    12421 tcagacgggt taattgttca tacttactgc atcgttccct tcacagattg aaacaatact
    12481 ctacttcata cctgtgagta tctgcgcagg cggggcattt tatactagcc tgttagcagt
    12541 atcgaacagt ttagcccgag tttaattcag cacaggctcc gatcaggatg atttattcat
    12601 agcaggaacc aacaaaaatg cgataaataa cagaccactg attagcagaa aggtaccgaa
    12661 aatgccatag tttgcgcctc cctcagcatc aattgactca ataatttggt tgaagccagt
    12721 atttccgatg tctggtcact tctactgcga ttaaaaaata aagcacttct ggaaatgaag
    12781 tcattcaccg tatggggaac agaaaccatt ttttcaccca ttctcgaaaa atgaaaatta
    12841 gttcgattat tgagaataga gccacacagg gcaataccaa tcgcgccccc aagattacgt
    12901 gttaaattaa atacaccaga ggctaacttc agccgttctt ttggaattcc ccccagcgtt
    12961 aatgttacga tgggggccat cgcaaactgc tgcgaaatcc ctctaattgc ctgtggaaac
    13021 agtaattcct gccatcccca ttcgtgggtt ataggggtaa aaagatacat tgaaaacaca
    13081 aatccgccca gccccgccat caacagccat cgcagattta tttttttact taaccagaag
    13141 taaaagggaa cagaaaataa ctggaaaata cctgttgtgc aaacggcaaa acctatctct
    13201 tcggcattta atccgcgaat ctgccccaaa aacacaggta ttaaatatac agtagagaat
    13261 atcccaaccc ctccagaaaa agaaaaaaaa catcccagag tgaaattttt atccttaaaa
    13321 gcatgaagat ccattatcgg attggatatt gtaagtgtac gcgcagcaaa taaaataaaa
    13381 ctcaccaatg ccagaactga tgtcagcaaa atagtattat catccagcca tccccagcgc
    13441 gctccttctt caagggtata ttccagacat cccagagtca tggccaacag gataatactc
    13501 ggataatcag caactttcag taaagagaga tcaggtttat caaaattaac cagaaaagga
    13561 atactcaaaa ctaaatatat gccaggtaat atattgatat aaaatagcca tcgccagtcg
    13621 aggttatcag tgagccagcc ccccagagtt ggccctagcg tcggtgacag tgaggccagc
    13681 gcactgacta ccgcagcagc caaccccaac tctttaccct ggtaataaat aaacgccatc
    13741 gtgaaaacta aagggatcat ggatgcgcca gcagctccct gtaaagcacg aaacaaaatc
    13801 atgatctgaa tattccatgc aagtccacac gcgatactca ttaaagtaaa tattcccgcg
    13861 gatagagtaa aaagccaacg agtggaaaat acccgtgaca gccacccaga gagcggaata
    13921 acaataatct cagcaattaa atagctggtc tgaagccagg ccatttcgtc tttacctgcg
    13981 gacagaccac cacctatttc atttagggat gacgatacaa tttgaatatc aagctgagca
    14041 ataaataatc ccatacacat actggcgaaa gcaatatgtt tacgcataga catcatctct
    14101 catcaacact gacggtgacg gacagtcccg ggcgaagaag ttgctttaat tcctttgcgt
    14161 cgtcaaatgc aatccgtacc gggacacgct gaacaatttt ggtgaaattg ccagtggcat
    14221 tttctatcgg aatcaagctg aaactggctc ctgtcgctgg ggaaaggctt tcaatgtgac
    14281 cgtgaaacac ttcccctttg agaatatcag cacggatctc agccttcatt cctggcttca
    14341 ttcccgctat ttggttttct ttataatttg catcaaccca caactcactg actggtacta
    14401 gtgaaactaa cgatgtgccg ccttccaccc aactgcctgt atgggcgctg cggtttgcga
    14461 caatgccatc
//
LOCUS       DAJTUP010000095        14180 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTUP010000095 DAJTUP010000000
VERSION     DAJTUP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14180
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.095"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            158..301
                     /locus_tag="OO468_003073"
     CDS             158..301
                     /locus_tag="OO468_003073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260099.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(520..2058)
                     /gene="aldB"
                     /locus_tag="OO468_003074"
     CDS             complement(520..2058)
                     /gene="aldB"
                     /locus_tag="OO468_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR6260100.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2223..3374)
                     /gene="yiaY"
                     /locus_tag="OO468_003075"
     CDS             complement(2223..3374)
                     /gene="yiaY"
                     /locus_tag="OO468_003075"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR6260101.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3564..5408)
                     /gene="selB"
                     /locus_tag="OO468_003076"
     CDS             complement(3564..5408)
                     /gene="selB"
                     /locus_tag="OO468_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR6260102.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5405..6796)
                     /gene="selA"
                     /locus_tag="OO468_003077"
     CDS             complement(5405..6796)
                     /gene="selA"
                     /locus_tag="OO468_003077"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR6260103.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(6894..7502)
                     /locus_tag="OO468_003078"
     CDS             complement(6894..7502)
                     /locus_tag="OO468_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR6260104.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7573..8658)
                     /locus_tag="OO468_003079"
     CDS             complement(7573..8658)
                     /locus_tag="OO468_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6260105.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8661..9023)
                     /locus_tag="OO468_003080"
     CDS             complement(8661..9023)
                     /locus_tag="OO468_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR6260106.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9560..11473
                     /gene="mtlA"
                     /locus_tag="OO468_003081"
     CDS             9560..11473
                     /gene="mtlA"
                     /locus_tag="OO468_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR6260107.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11568..12716
                     /gene="mtlD"
                     /locus_tag="OO468_003082"
     CDS             11568..12716
                     /gene="mtlD"
                     /locus_tag="OO468_003082"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6260108.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12716..13303
                     /gene="mtlR"
                     /locus_tag="OO468_003083"
     CDS             12716..13303
                     /gene="mtlR"
                     /locus_tag="OO468_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR6260109.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13315..13524)
                     /locus_tag="OO468_003084"
     CDS             complement(13315..13524)
                     /locus_tag="OO468_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260110.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13808..14170
                     /locus_tag="OO468_003085"
     CDS             13808..14170
                     /locus_tag="OO468_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR6260111.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3444 a         3788 c         3577 g         3371 t
ORIGIN      
        1 aacaattaac gcatattatg aaatgccatg atgcagtgca atttccttat ttatataacg
       61 acatattgtt ttttatatac tttctgaaat gtgctgtctg gtttttcaac aggacgaggt
      121 ttatcatatt gaaatagtgg aactattggc caaactaatg aataactcca gtaaaacatc
      181 cacagtacag attaagcgta ttaaaccttc aattatctac cgtttattgc tgattggcct
      241 cggatcacca atggtgattt acggcctggt tcgcccgctc accatcgaaa cgcgagatta
      301 aactcctgac aaaacacgcc ccagaaacgc acttctgttg gcgtagtctg ggttattgcg
      361 cacgtaggtt taaaacgtaa attgtaacaa cgtgcgcttt gtttatgccg gatgcggcgt
      421 gtacgcctta tccggcctac ggtcaggttc ccgtaggcat gataagacgc gtaagcgtcg
      481 catcaggcaa tgaataccca atgcgaccag cttcttatat cagaacagcc ccaacggttt
      541 atccgagtag ctcaccagca ggcacttggt ttgctggtaa tgctccagca tcatcttgtg
      601 ggtttcgcga ccgatacctg attgtttgta gccaccaaac gcagcatgtg ccgggtaagc
      661 gtgataacag ttggtccaca cgcgcccagc ctgtatgccg cgccccatct tataggccag
      721 attaccgttg cggctccaga cgcccgcgcc caggccatat tgcgtatcgt tcgccagctc
      781 cagcgcttct tccatcgttt tgaaggtggt caccgccagc accgggccaa aaatctcctc
      841 ctggaacacc cgcatattgt tctgaccaaa cagaatcgtc ggttcgaggt agtagccgtc
      901 tttcagttca ccttccagca gcttgcgccg cccgcctgtg agcacgtcag cgccctcttt
      961 tttaccgata tcaatgtagt tgaggatggt ttccagttgc ccgtgagaaa cctgcgcgcc
     1021 catttgcgtc acgctgtcga gcgggttacc gctgcgaatg ctttcgacac ggcggatggc
     1081 gcgttccata aagcgttcgt agattgactc ctgcactaag gcacgactcg gacaggtgca
     1141 aacttcgccc tggttaaagg caaacagtgc aaagccttcc agcgctttat cgaaaaaggc
     1201 atcttcttca tccatcacat cagcaaagaa gatatttggc gacttaccgc ccaactccag
     1261 cgtcaccgga ataatgtttt gcgttgcgta ttgcataatt tgttggccca cttccgttga
     1321 gccggtaaac gccactttgg cgatgcgttt cgaggtcgcc agatattcgc caatttcccc
     1381 acctgcgcca ttgaccacgt tcaccacgcc cggcggcagt aaatcaccga caatttccat
     1441 tagcagcagt acagaaagcg gggtaagacg tgcgggtttc agcaccacac agttgcccgc
     1501 cgccagcgcg ggagccattt tccagctcgc catcagcagc gggaagttcc acgggataat
     1561 ctgccccacc acgcctaacg gttcatggaa atgataggcc acggtttcgc tatcaacttc
     1621 actgatccca ccttcctgcg cccgaataca cgaggcgaaa tagcggaaat ggtcaatcgc
     1681 cagcggtaca tccgcagcac tggtttcgcg aatgggtttg ccgttatccc aggtttcagc
     1741 tgtcgctaac agctcgaggt tttgttccat tcgatcggca atcttaaaca gaatcgccgc
     1801 gcgatcctgc accgaggtgt gcgcccattt atctttcact ttgtgcgcag catccagcgc
     1861 cagatcgatg tctcgtttgc ccgaagacgc cacttcgcac agcagctgcc cggtcaccgg
     1921 cgtcagattc tggtaatact caccatcggc aggggctacc cattcaccgc caataaagtt
     1981 gtcgtagcgg gcttttaact tgatggggaa accatactcg ccgggcttaa tctgtgctga
     2041 agggggatta ttggtcatga tcgtctcctt gcggtgtgag gtataacaag ggtagacgtg
     2101 actggcgaaa tcttcgccag tcggtaacag ctttacgaca gctatcacga atttacgggc
     2161 aaggattaca gagatagttg aaaaagcggc tgacaatttg ccagccgttg ttggaatgat
     2221 gattacattg cggcgcgata aatcaccaca atttcttcgt gagttgcctg gatcgggtta
     2281 gtaaagccac aggcatcttt cagggcatta gtcgccagaa ccgcgaaatc ttcttctttc
     2341 acgttcaggt cgcgtaggcc tgccgggata tccactttct tcgccagttc acggatggcg
     2401 ttaatgcagg cttcagcacc ttccgcgtcg tttttacctg tcacgttcac gcccattgca
     2461 gcggcacagt cacgcagacg tgcagcggcg actttgctgt tgaatacctg aacatgcggc
     2521 agcagaacgg cgttacatac accgtgtggc aggttgtaga aaccgcccag ctggtgcgcc
     2581 atcgcatgaa cataacccag agaagcatta ttgaacgcca taccggcaag gaactgggca
     2641 taagccattg cttcacgcgc tttcgcatta ctgccatctt caacggctaa cggcaggttt
     2701 tcggcaatca tggtcacggc tttcagtgca caagcgtcag tgatcggcgt ggcggcaata
     2761 gaaacatacg cttcgatagc gtgcgttaag gcatccatac ccgttgcggc ggtcagtgac
     2821 ttcggcatac caatcatcag agaggagtca ttgacagaaa gcagcggagt cacgtgctta
     2881 tcaacaatcg ccattttgtt atgacgcgct tcgtcagtga tgatgcagaa acgggtcatt
     2941 tcagaggccg tacccgccgt ggtattgatg gcgatcatcg gcagctgcgg ttttgcagag
     3001 cggtcaacgc cttcgtaatc gcgaatatcg ccgccattgg ctgccaccag cgcaatacct
     3061 tttgcgcagt cgtgtggaga accaccgcct aaggagatca cgctatcgca attattttct
     3121 ttaagtaatt tcaaacctgc ggcgacgttt tccgtggtgg ggttaggttg ggtgccatca
     3181 taaataacgc taaaaatatt gcgttcttcc agtgcttttt gcacatcacc cgccatacct
     3241 aatttcgtta acatattgtc agtgacaatt aaggtacggg taaatccata atctgccatc
     3301 atattcattg catcagtcaa tgaatcagcg ccgatgacat tcacagaagg aataaagaaa
     3361 gttgaagatg ccataacaca ctcctgaaag tgtaaaaaag ggtgggcagc ttactgagga
     3421 ttttcattcg aaaacatgat cattattatt aattatcaaa attaatctaa taaaaagcac
     3481 attgtattca ttaaacaatg tgctttattt aaaatcataa gaaatttgta ttaatttatt
     3541 ttaaaaatta attaatcatt tccttatttt tccggaaata ataatgcgtc gcgcaataaa
     3601 tgatcatttc cacgacgacg cgtaaagcca atgcggtcaa aatattccag aatctgaatt
     3661 gccagctttc ggcctacgcc taagcgatcg cggaaatccg ccgcgcaggt tgaaccacac
     3721 tcctgatcga gatcgcggat catattggca aattcgacaa tccgatcgtt acggtaataa
     3781 cgatctttga cgatcgcggt aattattcct tgctgcgccg cctggcgtag cgtcaggcgc
     3841 attgcctgct cgtcggtacc cgtctctttt gccagatcac gcacccacca cggctcgtca
     3901 ccaaacagcg gctctgcttt ttgccagatg gcctgctgct cttcgctgaa gcccgctttg
     3961 tgatccggca gatgcagcca accgtgatga ctgaggatgt cgccgctttc gcgcatcttt
     4021 tcaatcagca acagtactag cgcttcatct tccattggca acgccatacg tcgcagacgt
     4081 tcgcgcccag gcccaggttc atcgcgatgt tgctcatgat aagtcgctaa tgtgtcgaga
     4141 attttccgtt gccagcgagc ggcaaccggc gcattcaaca agctataacc agcctgaata
     4201 tagccaggct gttgcagcag ttcgcgcatc ccttcgccgt tgagctggcg cgcccaagcg
     4261 aaatcaggaa gattaaccgc gccgcgttgc aggtgaacgg ctaacgcttc gctatcattc
     4321 tgtgccctgg cgagggaagc cagccattgc agatactctg gtttacgttt accgcgacgc
     4381 ggcgggttaa gcatcacgac gcgcgctccg gccagcgtgt tgcgggcaga gatatcgcgc
     4441 aataccaggc ggtcgttatc tgccagccat aacggggtgt cgaagaccag ttcagcaagg
     4501 ttatcttcca gcagtgaaac gcgtcccgtg acgtggctgg cggcgtggtg aatatgcagc
     4561 ggctgccact gggtcagcgg tgcatgggtt tgaagctcga caatcacccg tgtgaacggc
     4621 tctggcggca catcggcaag cagccagtcg ccacggttaa tctgctcttt ttccgcatca
     4681 cccgcgatgt caagcgcgat acgctgcccg gcattggcgg tttctgttgg ctggttttgc
     4741 gcatgcagcg cacgtacacg catcggttta tttacaccag tcagccagag tgaatcgcct
     4801 accttcactt ccccgcttaa cgccgtaccg gtgacgacca gcccggcacc ttttacggta
     4861 aatgcgcggt caatcgcgag gcggaaacta tgttggctgg cgtgctcgcg ttccggcaac
     4921 tgaagcagat gctcgcgcag ggcatcaatt ccccgacctt cggttgctgc ggtgataaac
     4981 agttttgcct cagcaaaacc gtattcccgc agaacctcct ttacctggcg ttcaacctca
     5041 tcaacacgcg cttcgtccac gcgatcggct ttggtcagcg ccactgtcag catcgggtta
     5101 ccggtcagct gcaaaatcgc cagatgctca cgggtctgtg ccatcacgcc gtcatcgcac
     5161 gccaccacca acagcgcgtg atcgatacca ccaacgcccg ccagcatgtt ggaaagaaac
     5221 ttttcatgac cgggaacgtc gataaaacca ggcacgcgac catccggctg cggccagtag
     5281 gcatagccga gatcgatggt catgccgcgc tttttttctt ccggcagacg gtcagcattt
     5341 acgccagtaa tcgcctgcaa taaggttgtt ttgccgtggt caacgtgtcc ggcagtcgca
     5401 ataatcattt caacaacatc tccaaaaacc gttgctcatc ttcaaggcag cgtaaatcca
     5461 gccacaatcg tccgtcataa atacgaccaa tcaccggcac tggcaattca cgccagcggg
     5521 cggctaatga ctcaaggtgg ctaccgcgtc catcatgggg tgtaaacgtt aatgccgcgc
     5581 tcggcaggcg atcaaccggc agcgaaccac tgccaatctg cgaaagacat ggcataacct
     5641 gtaccgcaaa ctccgcgccg taatgtgcga caaggggagc ctgtaaacgt tgtgcctgga
     5701 tttgaatgac ctctgcgctg cgggtaagca ggcgcagggt cggtaatttt tcactcagag
     5761 cttcagggtg taaataaaga cgcaacgtgg cttccagcgc cgcgagggtc attttatccg
     5821 cgcgtaatgc acgcttcagc gggtggcttt gcaggcgggc gatcatctct tttttaccaa
     5881 cgataatccc ggcctgcggc ccgcctaaca acttgtcgcc ggagaagctc accagactga
     5941 cgcccgccgc aatcaactcc tgcggcattg gctctttcgg caaaccgtac tggctaagat
     6001 cgaccagcga gccactgcct aaatcagtca ccactggaac atccagctct ttgccgagcg
     6061 ccaccagttc agcttcgtct atcgctttgg tgaacccctg aatgctgtag ttactggtat
     6121 gtactttcat caacagtgcg gtattttcat tcaccgcctg acgataatca ttcgcgtgcg
     6181 tgcggttggt ggtccctact tcgtgtaggg tgcagcctgc ctgacgcata acatcgggaa
     6241 tacgaaacgc gccgccaatc tccaccagtt cgccgcgaga taccaccacc tctttcccgc
     6301 tggcagtggc cgccaacatc aataacaccg ccgccgcatt gttattgacg atacaggcat
     6361 cttccgcccc cgtaatacgg cacagcagct gcgccagcgc ccgatcgcga tgtccgcgtc
     6421 cggcgtcgtc cagatcgtac tcgagggtca ctggcgaacg catagcctgc gcaacggctt
     6481 ccaccgcggc ttccgcctgt aaagctcgcc caaggttggt atgcagcacg gttcccgtca
     6541 ggttgatcac cggacgcagc gcgctctgcg cctctttcgt caaccgggca tcgacttctt
     6601 gcgcccagtt ttcacaccac gcaggcagcg tctggctgcc acgaatcact tctcgcgctt
     6661 cgtcgagcat ctgacgcaac aattccacca cgcgggtgtg accataagta tcacgcaaag
     6721 aaaggaagga gctatcgcgc aataagcgat caatagccgg aagttgacta tagagggaac
     6781 gcgtttcggt tgtcatagga aacctggctg atcaaggccc tctcacacgg agaagggcgt
     6841 ttaacataac cacggattgt aacgtgagat gggtcaggag gacatatcgc gcatcaagcc
     6901 tttggcggtt cggtgcgagc aaaactttcg cggctaaaca ggttttcgac cagtttgact
     6961 aagtgtgggc ggtcgacaca ccaacctggc gcaacgcggc ggaaattgag atatccgaca
     7021 gcacaggcaa tggcgatagt cgccagattg accgtatcgg ttttgagtgt gccatcgacc
     7081 agatatcctt ccagcacatc cagactgcgg ttgattttct cccgctggcg taacaattca
     7141 tcttcagact gctgcgccgc cggacgcgcc tgttcacgca ccgataccag cccggcatcc
     7201 ataatgccat ccgccagcgc ctcaattttg cgcacccgca acgactccag cggatcgcgc
     7261 ggcaacatcg ccggagcgac attcattaac tcaatatatt cagcgatgat cggcgaatca
     7321 aaccagcatt cgccctcttc ggtcaccagc accggcactt tccctaacgg gttaaattgc
     7381 gccacgccgt tatccgcgtt atagggcagt tcattaataa attcgaaagt tatgcccttt
     7441 tctaacaaca gaataaaaag tttgcgtaca aacgggctgg tgtagctacc gacgagtttc
     7501 atgccgagtc ctttgtgcga ggaaaaatat cagtatggct caggctcacg caaaacacag
     7561 gtgatataag tctcaatgat ccaaataaag ataatgcatc caactggtca ttcttagcag
     7621 cactttccgc atcaccgaaa catggctgaa atggtcggag taaaccgcca cctgggcgta
     7681 gatgccgtcg ggtaaggcat cgatatcatc gttagggtcc agttcaatgg ttcccagcac
     7741 accgtccgtg ccgggaacga ccgttaatga ttgcaatacc ccctgcgcct gataagaacc
     7801 gcctggcacg acaggtaaaa tactagtcaa tttgccgtgg aacacctgcc caggtagcgc
     7861 gttaaacacc acttccgcat catcgccagg tttcagacgt aacagcgagt tttgccgaaa
     7921 ttgggcgaca atttgccgtt tttgctcggg gatgaagacc atcaccggac gcagcggcaa
     7981 ggcagctgcg tatgtacctg ggcggatcag tacctgagtg acgtagccat tgcttggtgc
     8041 gcgaatgaca gtctgctcaa gattatattt tgcttcagta agttgcgctc ttaagctcac
     8101 aatctgcgat tgctcgccgt tgaccatact gtcgagctga ctctggatct gcgcctgctc
     8161 cgccaccgag cctttcacta acgcatcctg cgcgaggaaa ttttgccgcg catcgtcgat
     8221 gtcacgttcc gagaacggat tcaccgccgc ctggctgcct ttcaggtaac gttgataatt
     8281 tttaaacaga cggtcgcgct ccgctgaaac ctgggtggtg ttggcctgcg cttcagtgag
     8341 ctgcgcacgc agcgtcttta tattatgcgt cgccgtcatc aggtcagcct gaagtctgtc
     8401 aactcgcgcc tggtaacgaa ccgggtcgag cttaaaaagc acctcgccct tttgaataag
     8461 ctgattattc ttgtcagtga cttcagtaac aattcccgtc acctgtggcg tgatagggat
     8521 cgctatcact gccttttgcg cggtaaaagt gtaagggtgg ttgtagttca tcaacaaaat
     8581 caaaccactc accagaaaca cgcctcccag cgccgccgtc gccagcgcca cgtaagttaa
     8641 aacaatcaat agatccataa ttagatctcc gggaacgtgg aatagtcagt ctttttctcg
     8701 gcggcgagtt gatgctccag ccgggaaatt cggtcagaaa gtgcggcgat ttccggttca
     8761 gtcgctttct cctgcgacgc aacatgtgac tgcatacccc agccacgctc cggttgatag
     8821 agcgtcgccc agatccacag aaacggccag ataacatgca gagtaaacag gctcacccag
     8881 cccgccgtat gaatagcgtc ggcatggggg tgattgcgct ttttggcaat cagataaggg
     8941 atgtcatgta tcgcgatgat tccataaaaa atcaccagaa agacgaagat cagcactccc
     9001 aacgcgaaat agtttagaaa catatctgcc tcggattcac gtttatcagt gttgtttttt
     9061 ggctggcagc cagaagggag tcaggctgat attttgacaa taatccgggt tcgcgattct
     9121 cgccataaca ccaaagaata atttttagag gtgatgagtt gcttagttac ataacgattg
     9181 tatgacgaag gcataacatg ctgtagatca catcaggtga acgccgtaag aaaatatctt
     9241 gtgattcaga tcacaaagat tcaacaaacc atcaaaacaa aaatgtgaca ttactcacat
     9301 ttaaatgcca tttttagcga aaatcaccgc cttgtttctt ttttatacag gcgttttgtg
     9361 atgagcgtca cgtcaattac ctctctaccc cctatattta tgtgactgat atcacacaaa
     9421 agcccgtcga ctggacagtt aaccgattca gtgccagatt tcgcagtatc tacaaggtcc
     9481 ggctacctct gccgccacat taacaaaaaa cctcgggctt ccagcctgcg cgacagcaaa
     9541 cataagaagg ggtgttttta tgtcatccga tattaagatc aaagtgcaaa gctttggtcg
     9601 tttcctcagc aacatggtga tgccaaatat cggcgcgttt atcgcgtggg gtatcatcac
     9661 cgcgttattt attccaacag ggtggttacc gaacgagacg ctggcgaagc tggtcgggcc
     9721 gatgatcact tatctcctac cgctgctgat cggttatacc ggtggtaagc tggtaggcgg
     9781 caaacgtggt ggcgtagtcg gtgccattac caccatgggc gttatcgtcg gcgcagacat
     9841 gccgatgttc ctcggttcta tgattgcagg tccgctgggc ggctggtgca ttaagcactt
     9901 cgaccgctgg gtagacggta agatcaaatc cggttttgag atgctggtga ataacttctc
     9961 cgcaggcatc atcgggatga tcctcgctat tctggcattc ctcggcattg gcccgattgt
    10021 tgaagccctg tccaaaatgc tggctgcggg cgttaacttc atggttgtcc atgacatgct
    10081 gccgctggcg tctatctttg ttgaaccggc gaaaatcctg ttcctcaaca acgccattaa
    10141 ccacggtatc ttctcgccgc tgggtattca gcagtcccat gaactgggta aatcaatctt
    10201 cttcctgatt gaagctaacc caggtccagg tatgggcgtg ctgctggcgt acatgttctt
    10261 tggtcgtggt agcgctaaac agtctgcggg cggtgcggca atcatccact tcctgggtgg
    10321 tatccacgaa atctacttcc cgtatgtgct gatgaatccg cgtctgatcc tcgcagtcat
    10381 cctcggcggt atgactggcg tgttcacgct gactatcctg ggcggtggtc tggtttctcc
    10441 ggcatctccg ggttctatcc ttgctgtact ggcgatgaca ccaaaaggtg cttatttcgc
    10501 taacatcgcg ggtgtgtgtg cggcgatggc tgtctccttc gttgtctctg ctattttgct
    10561 gaaaaccagc aaagtgaaag aagaagatga tattgaagca gcaactcgtc gtatgcagga
    10621 catgaaagct gagtctaaag gcacatctcc gctatctgcg ggcgatgtga ctaacgacct
    10681 gagccacgta cgtaaaatca tcgttgcctg tgacgccggt atgggttcaa gtgcgatggg
    10741 cgcaggcgtg ctgcgtaaga aaattcagga tgcaggtctg tcgcagattt cggtcactaa
    10801 cagcgcgatc aacaacctgc cgccagatgt ggacctcgtc atcactcacc gtgacctgac
    10861 cgaacgcgct atgcgccagg ttccgcaggc acagcatatt tcgctgacca acttcctcga
    10921 cagcggcctg tacaccagcc taaccgaacg tctcgttgct gcccagcgcc atactgaaaa
    10981 cgaagtgaaa gtgaaagaca gtctgaaaga cagctttgac gattccagtg ctaacctgtt
    11041 caaactgggc gcggagaaca tcttcctcgg tcgcaaagca gcaaccaaag aagaagcgat
    11101 tcgttttgct ggcgagcagc tggtgaaagg cggttacgtt gagccggaat acgttcaggc
    11161 gatgctggat cgtgaaaaac tgaccccgac ttatctgggt gagtctatcg cggtgccaca
    11221 cggtacggtt gaagcgaaag atcgcgtact gaaaacgggc gtggtgttct gccagtaccc
    11281 ggaaggcgtg cgcttcggtg aagaagaaga tgacattgcc cgtctggtga ttggtattgc
    11341 agcccgtaac aacgagcaca ttcaggttat caccagcctg accaatgcac tggatgatga
    11401 gtccgtcatc gagcgtctgg cacacaccac cagcgtggat gaagtgctgg aactgctggc
    11461 aggtcgtaag taatccaatc ctaccctctc ctcacggaga gggtttgggt gagggaaaag
    11521 cctcacccca gccctctcgg gtaaaaacat tgatgaaggt taatactatg aaagcattac
    11581 attttggcgc aggtaatatc ggtcgtggct ttatcggtaa actgctggca gacgcgggta
    11641 tccaactgac gtttgccgat gtcaatcagg tggtacttga tgccctgaat gcccgtcata
    11701 gctatcaggt tcatgtggtc ggtgaaaccg agcaggtaga taccgtttcc ggcgtcaatg
    11761 ctgtcagcag cattggtgat gatgtcgttg atctgattgc tcaggtcgat ttagtcacta
    11821 ccgccgttgg cccggttgtg ctggaacgta ttgctccggc tatcgccaaa gggctggtga
    11881 aacgcaaaga acaaggtaat gaatccccgc tgaacatcat cgcctgtgaa aacatggtac
    11941 gcggcaccac gcagctgaaa ggtcatgtga tgaacgccct gccggaagac gccaaagcgt
    12001 gggtagaaga gcacgttggc tttgtcgatt ccgccgttga ccgcatcgta ccgccttcgg
    12061 cttcggcaac taacgatccg ctggaagtga cggtagaaac tttcagcgaa tggattgtcg
    12121 ataaaacgca gttcaaaggc gcactgccga acatcccagg catggagtta accgacaacc
    12181 tgatggcatt tgtcgaacgt aaactcttca ccctgaacac gggtcatgct ataaccgcgt
    12241 acctcggaaa actggccggt catcagacca ttcgtgacgc tattctcgac gagaaaatcc
    12301 gcgcggtggt aaaaggtgcg atggaagaaa gtggtgcggt actgatcaag cgctacggct
    12361 ttgacgcaga caaacatgcg gcgtacatcc agaaaatcct cggtcgtttt gagaacccgt
    12421 atctgaaaga tgatgtagag cgcgtaggcc gtcagccgct gcgtaaactg agtgctggcg
    12481 accgtctgat caagccactg ctcggtacgc tggaatacag ccttccgcac aagaatctga
    12541 ttcaggggat tgctggtgca atgcacttcc gcagtgaaga tgatccacag gctcaggaac
    12601 tggcagcact gatcgctgac aaaggtccgc aggcggcgct ggcacagatt tccggtcttg
    12661 atgccaacag cgaggttgta tccgaggcgg taaccgctta taaagcaatg caataatggt
    12721 ggaccaggcg caggacaccc tgcgcccgaa taacagattg tcagatatgc aggcaacaat
    12781 ggaacaaacc caggccgttg aaaaccgtgt gcttgagcgt ctgaatgctg gcaaaaccgt
    12841 gcgaagcttt ctgatcaccg ccgtcgagct tttgaccgag gcggtaaatc ttctggtgct
    12901 tcaggtattc cgcaaagacg attacgcggt gaagtatgct gtagaaccgt tactcgacgg
    12961 cgacggtccg ctgggcgatc tttctgtgcg tttaaaactc atttacgggt tgggcgtcat
    13021 taaccgccag gaatacgaag atgcggaact gctgatggca ttgcgtgaag agctaaatca
    13081 cgacggcaac gagtacgcct ttaccgacga cgaaatcctt ggaccctttg gtgaactgca
    13141 ctgcgtggcg gcgttaccac cgccgccaca gtttgaacca gcagactcca gtttgtatgc
    13201 aatgcaaatt cagcgctatc aacaggctgt gcgatcaaca atggtccttt cactgactga
    13261 gctgatttcc aaaatcagct taaaaaaagc ctttcaaaag taagcaacgt ctgcttactg
    13321 cccctctacc tgcttcggcc gataaagccg acgataatac tccagacgtt gtagatataa
    13381 cggcacgctt tcatcgggta ttccggacgg aatcgcgtta cggggaggaa gttttttcag
    13441 atactcccgg aacgcctggc ttgatgccat gaaatctacg gctttatcga taagaagcgg
    13501 aacgttttca cctaatttgc ccatgatgat atctccgtat taccccgcgc cgggaatgcg
    13561 cggccgccaa ttttagttta ggttcccggt aaagatgaca ttaaggaaaa cgtgctgaat
    13621 cctcaataat catccgctgc tgtttattat ctttttctat agatttctta attaatcaac
    13681 gaattgcgta gtttttgagc atgttaatga ttacgttgca gaactattac aatgtcatca
    13741 gttgtgaaca aagcacctgg tcgcgcatac taggggctat aaatttatct ttatcagaag
    13801 ccatcacatg aaagaagtcg aaaaaaacga aatcaagcgt ctcagcgatc gcctggacgc
    13861 catccgccac cagcaggccg atctgtcgct ggttgaagcc gcagacaaat atgccgagct
    13921 ggaaaaagag aaagccacgc tggaagcaga aattgctcgc ctgcgtgagg ttcatagcca
    13981 aaaactgagt aaagaagcac agaagctgat gaagatgccg ttccagcgcg caattaccaa
    14041 aaaagagcag gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg tggttgtgca
    14101 cccaatgacc gcgctggggc gcgaaatggg cctggaagag atgactggat tttctaagac
    14161 tacgttttaa gcccctcaga
//
LOCUS       DAJTUP010000096        14166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTUP010000096 DAJTUP010000000
VERSION     DAJTUP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.096"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1367)
                     /locus_tag="OO468_003086"
     CDS             complement(<1..1367)
                     /locus_tag="OO468_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6260112.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKE"
     gene            complement(1410..2784)
                     /locus_tag="OO468_003087"
                     /pseudo
     CDS             complement(1410..2784)
                     /locus_tag="OO468_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2830..4866)
                     /gene="yihQ"
                     /locus_tag="OO468_003088"
     CDS             complement(2830..4866)
                     /gene="yihQ"
                     /locus_tag="OO468_003088"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR6260113.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            complement(5065..5967)
                     /locus_tag="OO468_003089"
     CDS             complement(5065..5967)
                     /locus_tag="OO468_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR6260114.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            complement(6105..7346)
                     /gene="yihS"
                     /locus_tag="OO468_003090"
     CDS             complement(6105..7346)
                     /gene="yihS"
                     /locus_tag="OO468_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR6260115.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            complement(7363..8241)
                     /gene="yihT"
                     /locus_tag="OO468_003091"
     CDS             complement(7363..8241)
                     /gene="yihT"
                     /locus_tag="OO468_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR6260116.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(8265..9161)
                     /gene="yihU"
                     /locus_tag="OO468_003092"
     CDS             complement(8265..9161)
                     /gene="yihU"
                     /locus_tag="OO468_003092"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR6260117.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            9329..10225
                     /gene="yihV"
                     /locus_tag="OO468_003093"
     CDS             9329..10225
                     /gene="yihV"
                     /locus_tag="OO468_003093"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR6260118.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            10259..11044
                     /locus_tag="OO468_003094"
     CDS             10259..11044
                     /locus_tag="OO468_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6260119.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            11143..11742
                     /gene="yihX"
                     /locus_tag="OO468_003095"
     CDS             11143..11742
                     /gene="yihX"
                     /locus_tag="OO468_003095"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR6260120.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            11736..12608
                     /locus_tag="OO468_003096"
     CDS             11736..12608
                     /locus_tag="OO468_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR6260121.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            12605..13042
                     /gene="dtd"
                     /locus_tag="OO468_003097"
     CDS             12605..13042
                     /gene="dtd"
                     /locus_tag="OO468_003097"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR6260122.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            13087..14028
                     /gene="fabY"
                     /locus_tag="OO468_003098"
                     /gene_synonym="yiiD"
     CDS             13087..14028
                     /gene="fabY"
                     /locus_tag="OO468_003098"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR6260123.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         3466 a         3582 c         3706 g         3412 t
ORIGIN      
        1 atctctttat ttttgcgttg gttgatttcc tcaataatca atgcaaaccg tttttcattg
       61 agtgtgtaaa agaatcccat cgtcaatgca gcaataattg ccagtgcaca aggccagata
      121 aaaatgagct gacgcaaacc ctgtaatgtc gcatcgcttt gcgcgatgtt gggaacataa
      181 ccaatttgcg tcagcataat gcctggcaag aagccagcaa gtgcggcaga aattttacga
      241 aagaaggtat aaccggtata aacagaacct tcggcacgaa taccggtttt ccattcaccg
      301 taatctacgg tatctggtac cagtgcccag ttcaggctgt ttacaaaggc cgtgccaaaa
      361 aaggcgacac aagagaacat gacgaaagtg aaagagttac ttccccagaa ataattcagt
      421 atatcaccca ccgcccatag caccatgcca gctaaataaa cctgtttttt accaaaacat
      481 tttacagtta ctggcaccag taatacgccg acgaggatgc atcccatact gaagaatccc
      541 atccacgaca ataaattaat atcattcagc acatactggg tgtaatagac ctgaatcgcc
      601 agtttgatat taaatgccgc cagagtacac aggttagcaa tgcataaaac cagcaatggt
      661 ggattacgaa atatcgcgca aaaagatttg agaatactgg ctttatgtcc ggttggcgct
      721 gtgtccacat aatgctcttt gacgcctctg tagcacagca tcataaaaat aaagccgcca
      781 atggagaaca taagtgccgc acaggcataa ccgacggttg agtcagaaaa aagcgactgc
      841 aacggaataa acgccacggt acaaatcaat agccctatgg ttgcaccacc ctggcggtaa
      901 gccgcgagct gcgcgcgctc attcgggttt ttggtaattg ccgggatcat cgcaccatac
      961 gagcagttca tcagactgta tgaaagtcca aacatcataa acagcgtggt ggcaatcgtc
     1021 gttttaaccg gcaaacaaaa ggtggtggcg ataaactgga gcgttgcaat taacgccgcc
     1081 ggaactgctg catataaaat aaagggacga aatttgcctt taggcccaat atttttccgc
     1141 gagtcgagta aaaatccggt gagcatatcg gtaaacgcgg taaaaaactt cgcgaccaga
     1201 aagataatcc cgccatagta agcaggcatc cctaattcat cggtataaag tttgaggaga
     1261 tacagagtac cgatgcagag cattaaattc gaaccgacgt cgcccatacc ataggcgatt
     1321 ttttcccgca gattcagttt taatgtcagt ggattatggt cagacataat cactccttat
     1381 aacgccccgc agggcgtcgt ttattattat tacgccgtgc gtttacgggc ttctatctct
     1441 tccacaatgc ggacatacat cttctcgttc aggctgtaga agcaacccat cgccacaatg
     1501 gtgactaccg ccagtgcgct tgggtagatg aagatcaact ggcgtaaccc ttcaatggtg
     1561 tggtcagcct gtgcgacgtt cggcacatag ccaatttgcg tcagcatcca gccggggaag
     1621 aaaccagcca gcgcctgaga cactttgcga aagaaggtga agccggtgta gaccgtgcct
     1681 tccgaacgca cgccggtgcg ccactcgccg tactcgacgg tgtcggaaac cagcgcccag
     1741 ttcaggcgct gagccgaaga acgccaggca ggagaacgcc acaaagctga ccgagccgcc
     1801 gccgaagaaa tagttgagca gatcgcccag cacccaaatc agcaggccgc cgatatagac
     1861 cttcttctta ccaaaacgtc tgactgcgcc aggcatcagg aacacgccga tgaaaataca
     1921 gcccatgctg aaaaatccca tatacgacaa caggatggga tcgttaagta cgtactgggt
     1981 gtaatagacc tgaatggcga gcttgacgtt aaacgcccct aaggtgcaga ggttggcaat
     2041 acacagaatg aacagtgggc ggttaccggc aattgcgcgg aaagattgca acaggccagg
     2101 cttttgcgcc ggattggttg gttgggtttc gacataacgc tctttcacgc ctgagtagca
     2161 gatccacata aacagcaggc cgaacagtga aaacagcgtg gcggcgaaga tatagccaag
     2221 tttctgatta ccttcgataa gattcataac tggcacgaat cccaccgtgc acagcagcag
     2281 gcccagcgta gcgccgccct gacgccatgc cgccagtgag gcgcgttcgt tggggttttt
     2341 ggtgattgcc gggaccattg cgccgtagga gcagttcatc atgctgaaaa acagtccgta
     2401 aagcataaac agaatagtgg ccatcaccgt tttaccggtg acatcaaacg gtgtgccgac
     2461 aaagttggcg atcgccagta aggtgaccgg gaatgacgca tacagaataa aaggacggaa
     2521 cttacctttt ggaccgatct tgcgtcggga atccaacata atgccggtac ccatatcggt
     2581 aaacgcagta aagaattttg aaatcaaaaa gataatgccg ccataggtgc ctggcagccc
     2641 cagaacgtcg gtataaaact tcaaaagata aagcgtaccg atatccagca ggatgttaga
     2701 ggccaggtcg ccaataccgt aagagagttt ctctttaaag ggcaggcgta aagttgctgg
     2761 atcttccgtt gtgatgtgac tcatcgatgt tctccctttg cccatgagtt tccccacggg
     2821 cggatgtgat tagatgctgt ttaacgacgc gaacagtgcc gcccattcgc tatcggcgcg
     2881 ataaaagacc ggcggcttgc cgatgggcgc attaacggta acttccccgc cccggaacgc
     2941 ttcacccgtc caggcatgta cccagttatc ctccggcaga tagagcgtcc agtcgctgcg
     3001 accttcttcg tgtaccggag cgaccagaat gtcgcggcct aacaggtact gatatttcag
     3061 ggaataggta tgtgcatcgt cttcgtaatg caggaacagc gggcgcatga ccggcagacc
     3121 ggacttcgca ttcagcgcga cggcctcttt cagataaggt ttcagggtgg tgaaaacggt
     3181 agtcatacgg gcaaaatggg cgatagtttc cgcatcgccg tcaaactgcc agttgtcgcc
     3241 aggacggtta ccttcgtggg tgcgcatcat cggcgtgaag gcgctgaaat cgcaccagcg
     3301 cagcagcagc tctttgctgc gcttcatctc aaacagggtg gtgtaaccgc caatgtcgct
     3361 gtgatgcaga ccatgtccgg tcattgccag cgacagcgcc gccgggacta cggacgccag
     3421 gccatcgtcg agactccagt ccacgttctg gtcgcccgcc cacatcatgg tggagtattt
     3481 ctggctaccg gtagaaccgg cgcgcataaa gaagaggatc tcgccgagct tgcccgtttc
     3541 ttcaagggct tcgtagttac acttcgccca cagcgcaggc caggcgttat gcataatttc
     3601 ggcactgacg ccgttatgca ggtacgtgtc ggtgggcaga tactcgccga agtcagccat
     3661 ccagccgccg cagccgagtt caatcatgtt ctttttgatc acttccttga accaggcgta
     3721 ggcttctgga ttagtgagat cgacaacgcc gccgtaaaac tcgccaaact ccaccagata
     3781 gtcaccgcca gaggcatctt ttgctagata gccgtgttgt gccgcttctt cgcagagatc
     3841 tttatcgctg gcaacatacg ggttgatata ggccaggaac tgcacgccct cctgatacca
     3901 ctgcttaatg cgtgaatcca gttgcgggta gttttcgctg ttccacttcc agttccacat
     3961 cacgcgcttg ccaaaagagg ttatacggat cccagaccag tcctgcgccc agatgccgtt
     4021 aaccttcacg cccgcgttac gcatggtgtc cagtttcttc tggcacactt ccgtcccgcc
     4081 ctgaatgccg agcgttaccc cgtcataaat ccagtcgggc agttctggct ggcgtcccag
     4141 cagggcagtt aatttttcca gcagggagat gtatgtgtca gcacattcaa aacgtagcgt
     4201 agctttgtct tcccacagcg ccagttcatg gtattccggg gcgctaaagt cgaagttcat
     4261 gtagcaactg ttatcaacat ggcagtaata cttctgcgtg ctgacaaacg taggctgtgg
     4321 gaagaaagtc cagtaatagt cgccgcccgc attttctttg cagtcggcct gccaggtgac
     4381 ataggtttgt ttgttgcgac caacgccttg ttcactggtc catagcggga acggtttgcc
     4441 gcgcagatcg aagtaggaaa actgttcgcc gcagccgtag atatggtcct ctggttgagc
     4501 ggcaaggcgc agccagatac ggttgtggtt aaggttgtcg ttttgtagtt ccagcaataa
     4561 acggccctga tcgtcggcag agatattcag cgtggcgcta atgtcagaac cacggctgaa
     4621 atgaattaac caaccatccg gtgactggct gacgatggcg tcggtaagcg caattttctc
     4681 ctgtagttta tctttaatgc tgaaattacc gcggaacata tcgatatccg ctatacctga
     4741 gccaatccat aaacaaggat tatctttgtt atgggttaaa ataagacgtt gttgaaaatg
     4801 tagggtaaaa ctgtcgttat tctgatgaaa ttgaaaatct aataactgtg gacgtggcgt
     4861 atccataact aatctccata gagttgagaa cgagattgtt gccttcctgt tattacaggc
     4921 ttatgtaaaa tgattctcat aaaactgata tataaaaact ttacagaagc tgagctgttt
     4981 ttaacttaat aaattgaaga tacataatgt tgcctttagc gagtgaaatg ttcttcaatg
     5041 attttgactg ctaatcaata caagttattt tctctgtccg taaatattaa aaaacaggcg
     5101 gtgcggtttt cctggttcca gtaataataa gtcgatacct gaattaaagg cgttaggcgg
     5161 gcaactcata ggttcaactg ccagtccctg acgctgtaat ttttcaccgc tataaacctg
     5221 cacccagggt tgatcggaac aaaggcttac cgaaagcgcc tgctgtggat gggtaatggt
     5281 catttcccac aaatcattag cggttttaaa ggtgtggtcg atttttgtcg cggctatttt
     5341 tttcgcctgc gtgaaattta aatccagctc atcaacgtgg tgaagcgttg tcggatttac
     5401 gtgttcatcg acggcataaa cctggtttgc cggaagctgg aacagatatt catcaacaga
     5461 tgttaagttg caggttaaat aaggatgaat acccacgcca taaggcgctg cgacagtgcc
     5521 gatattctgg cttgcgatct caacggaaag acccgtgtgt gcgttgagcg aataaacaac
     5581 ttgtgaagcc agcataaacg gatagccgta actgggcggc agaaaagctg tcagggtaac
     5641 gctggtggtg gttaattcac taatttgcca gtctcgccag gcaagcagcc cgtgaatagc
     5701 ggcttttgaa ctatgttcat taatgggtaa ctgatattcc tgcccctgat aacggtaaca
     5761 gccgttggcg atccggttcg gccacggaat aagcacttta cccagatgcg ccagcggcat
     5821 ctcttcgggt ttgtgcggga tgactaaatg acaaccctga aaggttaatt cagccagacc
     5881 agcaccgacg gtcacaatgg tcgcgtgata atcccccgcg gcaagtgaaa cggtctgtcc
     5941 actgcaatgc atattggtga tttgcataca aggcacctta attaacgaca tacagcctcc
     6001 cgtgaggctg atgtcaaacc caaaaactgc accggacggt cccctcgccc ctttggggag
     6061 agggttaggg tgaggggaaa acccgcaccc tattttcaga tcagttattt cgcattaata
     6121 tccagcagac ccgctgcaac cgctggagcc atgccgggcg ctaacgggat acgcgggatc
     6181 accaggcaat gcagcaggtg ataaatatcc tgtttgccgt cccagacttt ggtgatgacc
     6241 ttattgtccg catccagctc ctgccaccag gaaccatttt catagtccat caggtactta
     6301 atgcagtact cccaccatgt ttgataccag gtttcatact ggcgatcgcc agtgacggtg
     6361 tagagcgcgt agaccgtacc cattgcttcg acgataggcc aacgtacacg ttcgcggacc
     6421 accggttttc cttcccagtc aacggtataa acaatcccgt ccgctccatc gggtgcccag
     6481 gcatcgcgca cggtggcgtt aaacagacct ttggcatctt ctagcagcca tgctggtggt
     6541 tgttcgcaac gggcttccag ggcagcatgg atgtgcagca ttaaacggcc ccattcgatc
     6601 cagtggcctg gtgtacctcc gaacgcgcgg aagcgatgcg ccgggttatc tttgttgtaa
     6661 tccggcagcg gattccactg ggtatcgaag tgttcgttaa cgcgataatg attatttctg
     6721 gcgacgtcgt ggataatcac ggaagccacg cgaatcgcgc gatccagcca ttttttgtcg
     6781 tgagtgacgt cataaacaat caagaaagct tccaccgcgt gcatattggc attgccgccg
     6841 cggtactctt cggttttgct gaaggcttcg tcccaggact ccaggcacat ctgctcttct
     6901 tcgctccaga aatatttctc gataatctca atggtgtaat cgagcagctt gcgcgcttcc
     6961 gggtgacccg ttgtgacggc gctggcggca cccagcagag caaagaagtg ttgatagccc
     7021 tgtttggagg catccaccac gccctcgtca ttcacgcagg cataccagcc gccatatttt
     7081 ttatcacgca gtgcgccgtt catggcttta ataccgtgat caaccaacgc gtaagcgcca
     7141 ggtcgaccca tcgccgcagc aacggaataa acgtgcaaca tacgagctgt gatccacaga
     7201 tgggtgccca tctcttcttt gatttgccct ttattgccta accagccaaa accagtcggc
     7261 actacggaat ttttaccaaa atcaaagatg cggtcggttt cctgttccag ccaacggttg
     7321 tggcttaggg tgttaaacca tttcattcct tttatcctca tcttagcggc gtttagccat
     7381 catttcgtcg acgatatcgc caagttgttg taatttcggt gcgcaaacgt cacggagcat
     7441 cagctcgttg tctggtaaac cgacgaccga tgcccagact gcacggcctg ccaggaatcc
     7501 cgatgcgccc gccgtcattg ccacgcgtac ggcacgcggg aacagttttt cgtcgacact
     7561 ggaagagagg atcacccatg gcatattgat atggtcattc agacgttgtg aagcacagag
     7621 aagctcttgt tgcggacctt tgccataaag cggcatttca actttgtaga ggtcagcgcc
     7681 actgtcgccc agctctttgg cggcatcgat gatcgcttgt tcgcgatcga atttatcgcc
     7741 acgacgcggt gggcggacga ctggctcaat gatgcttacc agaccgtgtg agtggcacag
     7801 ttcgttgaac tctttcacca tatccagacg ttgctgcgca tcttcatcac tacgccacag
     7861 caccagcagt tttaaggctt tgccaccgtc ttgtttgatc tgtagcggat tgattttgcg
     7921 gtcaataacc acgctatcga ccggaatacc gttgccagga atgaactcat cggcggcgac
     7981 aatcatggcg caacttttgg caatcgcgtt ttgctcaacc acctggcggt agcagaattg
     8041 ttgatctacc agaatcgccg aggcataagg cgagagggcc tttgcagcgt taactttgaa
     8101 atcagttaaa acgctatcgg ctaccggagc gggtgcccca gccgcggcaa acatcatgcg
     8161 catggcttcg cgctgatcga ccgccagcat ggcaaaaccg cccgatgcgc gcgtaatgtc
     8221 gttgatggtg tacttattca ttcatttatt cctttatcca gtcgttacat ttttactttg
     8281 gcagtcatcc cggcactgac acggacctgt tccagaatgg cggaccagtc ctggcgaccg
     8341 cgacccgctg cgcgcgcctg gctataaacc tcccgtgagg cggcccccag cggcattggc
     8401 acatgcagct ggttggcgac atcaagggcg atgccaagat ccttatgggc aagatcgatc
     8461 atgaaggcgg gagaaagatc tccgctgagg actttgtttg gccaggaagt ggtgaagtga
     8521 cctttaccgg cggcggtacc gctcatcact ttgacggcaa catcgaaggg aagattcagg
     8581 gcttcgcaca aaacggcggc ttctgccgaa agcgcattga gcgcgatgct catgtagttg
     8641 ttgatgagct taacgcggat ccccatgccc ggaccgccag cgttgatcaa ctcactgccc
     8701 atcgccatca ggatcggtgt ggcacgttca acttgttcag cggtgccgcc agccagcagt
     8761 aacagagtac cggtaatggc atttgcagaa gtacggccta ccggaacatc catcatgctg
     8821 aagcctttgg cttgcatatc ggcaatcaat ttatcggttt gcagcggatg gatggtggac
     8881 atatcaatga ccagcgcatc ggtagataag ccttcgcaaa cgccgttttc accgaacaac
     8941 acgttgcgca ccagatcgcc attcggcaac atggtaatga taaattcggc atctttagcg
     9001 gcctgcgccg ggttggcggc gggagtcgca cctttgtcta ccagatgccg cacagcttcg
     9061 gcgttcacat caaagacgcg aagttgatgc ccttgctgca ataaattgct cgccattggc
     9121 gaacccattt gtcctaaacc gataaacgcg attgctgcca taaccctctc ctgaatacag
     9181 tgatgtcact ttttgtcatt tatgacatgc tttgcttgtc tgtttttgat cgaatttgta
     9241 atttatcgtc aaaaaattga cagccgtcac tttttaaaca attggtgaaa ttaaaatgaa
     9301 cgcatcccaa aatgtttaag gaatgaccat gattcgtgtt gcttgtgtag gtataaccgt
     9361 gatggatcgc atctattacg tggaagggtt accgacggag agcggtaaat acgtggcgag
     9421 aaattatacg gaagttggtg gcgggccagc ggcgacggca gcggttgcgg cggcaaggct
     9481 gggggcgcag gtcgatttta ttggtcgcgt aggtgatgac gacgcgggca acagcctgct
     9541 ggcagagctg gaatcctggg gcgttaacac ccgttacacc aaacggtata accaggcgaa
     9601 atcttcgcaa tccgccatca tggtggatac caaaggcgag cggataatca ttaactaccc
     9661 cagcccggat ctgctgcctg acgcagagtg gttggaggaa attgatttct ctcagtggga
     9721 tgttgtgctg gcagatgtac gctggcacga cggcgctaaa aaagccttca ccctggcccg
     9781 tcaggcgggt gtgatgaccg ttctggacgg ggacattacg ccgcaggata tcagtgagct
     9841 ggtggcatta agcgatcacg cggccttttc agaaccgggt ctggcgcgct taacgggcgt
     9901 gaaagagatg gccagtgcgc taaaacaggc acaaacgctc acaaatggtc atgtctatgt
     9961 gacccagggt agcgcaggct gcgactggct ggaaaatggt gggcgtcagc atcaaccggc
    10021 cttcaaagtt gatgtggtag ataccacagg tgcgggagat gtttttcacg gcgctttggc
    10081 ggtggcgctg gcaacaagtg gggatttagc ggagtcagtc cgcttcgcca gcggtgtagc
    10141 ggcgttaaaa tgcacacgtc ccggtggacg agccgggatc cctgactgtg atcaaacccg
    10201 atcttttttg tcactttttg tataaaatgc cagggtgatg gtttttcgag gaattttcat
    10261 gagccttacc gaactaaccg gtaacccgcg gcacgaccaa ctcctcatgc tgatcgccga
    10321 gcgtgggtat atgaatattg atgagctggc aaatctgctg gatgtctcca cgcagacggt
    10381 ccgccgggat attcgtaaat taagcgagca aggcctgatt acgcgccatc acggtggcgc
    10441 gggtcgggct tccagcgtcg ttaatacggc gttcgagcag cgtgaggttt cgcaaaccga
    10501 gaaaaaaaaa gcgattgccg aagcggtggc agactatatt cctgatggtt caacaatatt
    10561 tatcaccatt ggtacgactg ttgagcatgt tgcccgggcg ttacttaacc ataatcattt
    10621 gcggataatc accaacagcc tgcgtgtggc gcatattctt taccacaacc cgcgctttga
    10681 agtgatggtg cccggcggta cgttgcgctc tcataatagc gggatcattg gcccttcagc
    10741 ggcgtccttt gtggctgatt ttcgtgctga ttatctggta acaagcgttg gggcgattga
    10801 gagcgatggc gcgttgatgg agtttgatgt aaacgaagct aacgtggtga aaacgatgat
    10861 ggcgcacgcg agaaatattc tgctggtcgc cgatcacact aagtatcatg cttcggcggc
    10921 ggttgaaatt ggtaacgtgg cacaggtcac tgcgctcttt accgacgagc tgccgcccgc
    10981 tgcgctaaaa tcacgcttac aagacagcca aattgaaatc attcttcccc aggaagacgc
    11041 gtagattttt gtgaccgtta acctggcttc ataccttgcc acatagccaa acccatcctt
    11101 tcccgctaca gttaatttct tgtggcgcga aaggaggcaa aaatgctcta tatctttgat
    11161 ttaggtaatg tgattgtcga tatcgacttt aaccgtgtgc tgggagcctg gagcgattta
    11221 acgcgtattc cgctggcatc gcttaagaag agttttcata tgggggaggc gtttcatcag
    11281 catgagcgtg gggaaattag cgacgaagcg ttcgcagagg cgctgtgtca tgagatggct
    11341 ctaccgctaa gctacgagca gttctctcac ggctggcagg cggtgtttgt tgcgctgcgc
    11401 ccggaagtga tcgccatcat gcataaactg cgtgagcagg ggcatcgcgt ggtggtgctt
    11461 tccaatacca accgcctgca taccaccttc tggccggaag aatacccgga aattcgtgat
    11521 gctgctgacc atatctatct gtcgcaagat ctggggatgc gcaaacctga agcacgaatt
    11581 taccagcatg ttttgcaggc ggaaggtttt tcacccagcg atacggtctt tttcgacgat
    11641 aacgccgata atatagaagg agctaatcag ctaggcatta ccagtattct ggtgaaagat
    11701 aaaaccacca tcccggacta tttcgcgaag gtgttatgct aaaaaccatt caggacaaag
    11761 ccaggcaccg tacccgtcca ctatgggcct ggctaaaact actctggcaa cgtattgatg
    11821 aggacaacat gacaaccctg gcaggtaacc ttgcctatgt gtcgttgctc tcattagtgc
    11881 cgctggttgc cgttgttttt gcgcttttcg ccgcttttcc catgttttcc gacgtcagca
    11941 ttcagttacg tcactttatt tttgccaact ttctgcctgc tactggcgat gttattcagc
    12001 ggtatatcga acaatttgtt gctaattcca acaagatgac cgccgttggg gcgtgcgggc
    12061 tgatcgtcac cgcgttattg ttgatgtact ccatcgatag cgcgttgaat accatctggc
    12121 gcagtaaacg agcgcgacca aaaatttact cgttcgccgt gtactggatg attttaacgc
    12181 tggggccgct gctggcaggg gccagtctgg cgatcagttc ctatttgctc tccctgcgct
    12241 gggcgagcga tctcaatact gtcatcgaca acgtgctgcg tatttttccg ttgctgttgt
    12301 cgtggatctc cttctggttg ctgtacagca ttgttcctac catccgcgta cctaaccgcg
    12361 acgcgattgt cggcgcgttt gtcgccgcac tcctgttcga agcaggaaag aaaggtttcg
    12421 cgctttatat caccatgttc ccgtcatatc agctcattta cggtgtgctg gcggtgatcc
    12481 ccattctctt tgtttgggtc tactggacat ggtgtatcgt cttgcttggc gcggaaatta
    12541 ctgtcactct cggggaatac cgcaaactaa aacaagcagc tgaacaagaa gaagacgacg
    12601 aaccatgatt gcattaattc aacgcgtaac ccgtgccagc gtcaccgtgg agggagaagt
    12661 gacgggcgaa attggcgcgg gacttttggt gttattgggt gtcgaaaagg atgacgacga
    12721 acagaaagca aaccgtctgt gcgagcgtgt gctcggctac cgcatcttta gcgatgccga
    12781 aggcaagatg aatctcaacg tgcaacaggc gggcggcagt gtactggtgg tttcccagtt
    12841 taccctcgcc gcagataccg aacgggggat gcgcccaagt ttctccaaag gcgcatcacc
    12901 ggatcgcgca gaggcgttat atgactattt cgtcgaacgc tgccgccagc aagagatgaa
    12961 cacgcaaaca ggacgcttcg ctgccgatat gcaggtatcg ctggtcaatg atggccccgt
    13021 gacattctgg ttgcaggtat gagccagctt ccagggttgt cacgggaaac aagagagagt
    13081 atcgctatgt atcaccttca ggttccacaa acagaagaag agttagagcg ttactatcac
    13141 tttcgctggg aaatgttgcg taagcccctg catcaaccaa aaggttcgga acgcgacgcg
    13201 tgggatgcga tggcgcatca ccagatggtc gtcgacgagc agggtaatct ggtggcggta
    13261 ggccgattgt atattaatgc cgacaatgaa gcgtccattc gctttatggt cgttcacccg
    13321 gacgtgcagg acaaagggtt aggaacgctg atggcgatga ccctggagtc ggtggcgcgt
    13381 caggaaggcg ttaagcgcgt gacctgtagc gcccgtgaag acgcggtgga gtttttcgcc
    13441 aagctggggt ttgttaatca gggagaaatc accacgccaa ccaccacgcc gattcgccat
    13501 tttttgatga ttaagcccgt cgccactctg gatgatattt tgcatcgcgg cgactggtgc
    13561 gcgcagctgc aacaggcgtg gtacgaacat atcccgctta gtgaaaaaat gggcgtgcgt
    13621 attcaacaat ataccgggca aaaatttatc accaccatgc cggaaaccgg caatcagaat
    13681 ccgcaccata cgctgtttgc cgggagttta ttctcgctgg cgacgctcac cggttggggg
    13741 cttatctggc tgatgctgcg tgaacgccac ctcggcggaa cgattattct ggcggatgcg
    13801 catatccgct acagcaaacc gattagcggt aaaccccatg cggtagccga ccttggtgcc
    13861 ttaagcggcg atctcgaccg tctggcgcgc ggacgaaaag cacgggtgca gatgcaggtc
    13921 gaaatctttg gcgacgagac gccgggtgca gtgtttgaag gcacgtatat cgttctgccc
    13981 gcgaagccat ttggcccgta tgaagagggc gggaacgaag aagagtagct tatggagagg
    14041 ggcggtgctg cctctctcat tcattccggt catttacatc agtccctgac tattccggtt
    14101 atattgaata agaactttat aaatttttat tcttcgttgg caccatacgt gtcttgctac
    14161 atcact
//
LOCUS       DAJTUP010000097        13929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTUP010000097 DAJTUP010000000
VERSION     DAJTUP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.097"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..278)
                     /gene="csgB"
                     /locus_tag="OO468_003099"
     CDS             complement(<1..278)
                     /gene="csgB"
                     /locus_tag="OO468_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR6260124.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAY"
     gene            1032..1682
                     /gene="csgD"
                     /locus_tag="OO468_003100"
     CDS             1032..1682
                     /gene="csgD"
                     /locus_tag="OO468_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR6260125.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            1687..2076
                     /gene="csgE"
                     /locus_tag="OO468_003101"
     CDS             1687..2076
                     /gene="csgE"
                     /locus_tag="OO468_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR6260126.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2101..2517
                     /gene="csgF"
                     /locus_tag="OO468_003102"
     CDS             2101..2517
                     /gene="csgF"
                     /locus_tag="OO468_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR6260127.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2544..3250
                     /locus_tag="OO468_003103"
                     /pseudo
     CDS             2544..3250
                     /locus_tag="OO468_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3383..3874)
                     /locus_tag="OO468_003104"
     CDS             complement(3383..3874)
                     /locus_tag="OO468_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR6260128.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3976..4530)
                     /gene="ycdY"
                     /locus_tag="OO468_003105"
     CDS             complement(3976..4530)
                     /gene="ycdY"
                     /locus_tag="OO468_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR6260129.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4554..5291)
                     /gene="ycdX"
                     /locus_tag="OO468_003106"
     CDS             complement(4554..5291)
                     /gene="ycdX"
                     /locus_tag="OO468_003106"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR6260130.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5346..6284)
                     /gene="ghrA"
                     /locus_tag="OO468_003107"
                     /pseudo
     CDS             complement(5346..6284)
                     /gene="ghrA"
                     /locus_tag="OO468_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6519..6606
                     /locus_tag="OO468_003108"
     tRNA            6519..6606
                     /locus_tag="OO468_003108"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6553..6555,aa:Ser,seq:gga)
     gene            complement(6756..7037)
                     /locus_tag="OO468_003109"
                     /pseudo
     CDS             complement(6756..7037)
                     /locus_tag="OO468_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7319..8323)
                     /locus_tag="OO468_003110"
     CDS             complement(7319..8323)
                     /locus_tag="OO468_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR6260131.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(8395..8955)
                     /locus_tag="OO468_003111"
                     /pseudo
     CDS             complement(8395..8955)
                     /locus_tag="OO468_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(9040..9237)
                     /locus_tag="OO468_003112"
     CDS             complement(9040..9237)
                     /locus_tag="OO468_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260132.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(9249..9740)
                     /locus_tag="OO468_003113"
     CDS             complement(9249..9740)
                     /locus_tag="OO468_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260133.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(9737..10111)
                     /locus_tag="OO468_003114"
     CDS             complement(9737..10111)
                     /locus_tag="OO468_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR6260134.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(10158..10532)
                     /locus_tag="OO468_003115"
     CDS             complement(10158..10532)
                     /locus_tag="OO468_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR6260135.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(10695..10916)
                     /locus_tag="OO468_003116"
     CDS             complement(10695..10916)
                     /locus_tag="OO468_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR6260136.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(10979..11455)
                     /locus_tag="OO468_003117"
     CDS             complement(10979..11455)
                     /locus_tag="OO468_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR6260137.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(11471..11944)
                     /locus_tag="OO468_003118"
     CDS             complement(11471..11944)
                     /locus_tag="OO468_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR6260138.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(12286..13107)
                     /locus_tag="OO468_003119"
     CDS             complement(12286..13107)
                     /locus_tag="OO468_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR6260139.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(13207..13380)
                     /locus_tag="OO468_003120"
     CDS             complement(13207..13380)
                     /locus_tag="OO468_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR6260140.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            complement(13500..>13929)
                     /locus_tag="OO468_003121"
     CDS             complement(13500..>13929)
                     /locus_tag="OO468_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260141.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
BASE COUNT         3559 a         3408 c         3347 g         3615 t
ORIGIN      
        1 atatatgcaa ggttataatc tcctgtctgg tcaatctttg cccgattgct actaccttct
       61 tgcgcaacaa ccgccaaaag ttttgagcct ccctgccgta actgagcact attattagtc
      121 ccagcctgac caattatggc tgcctgatta aatgaagact tactcaattc atttaccgcg
      181 aagttatatt ctgaattagc taaatcataa cctgctgcgg ctgcaatccc aggcgcaccc
      241 agtattgtta acatcataaa taacaatttg tttttcatgt tgtcaccctg gacctggtcg
      301 tacatttaag aaattaaatc atttcaactt ggttgttaac gcaacctgta ttttgttaac
      361 gctgcgttac gatggaaagt atgtctgcgg aaatattttt aataactcac ccgcgcgttg
      421 tatttttctt tttttgttca taccaaagta ttaaaacccg cgtaaatacg ctgttatcta
      481 cgcaaaaaat attttgtttt ctcttaaatc tccgttttcc gctatcaaaa atcaccagac
      541 agtcattctt cttgcccgtc gctgattgct gcgatatgtc ttgcgcacaa gccgtgacaa
      601 acctcgtctt tttgcagtaa aaaaattgtc cacggaggtg tggagaaaaa acaaaaacgt
      661 tttacatgac gaaaggacta caccgaaata ttttttatat gtattattag taagttagca
      721 ccatttgtat gattttttaa aattgtgcaa taaaaaccaa atgtacaact tttctatcat
      781 ttctaaactt aataaaacct taatgttaac attttaatat aatgagttac attttgttac
      841 atgtttaaca cttgatttaa gatttgtaat ggctagattg aaatcagatg taatccatta
      901 gttttatatt ttacccattt agggctgatt tattactaca cacagcagtg caacatctgt
      961 cagtacttct ggtgcttcta ttttagaggc agctgtcagg tgtgcgatca ataaaaaagt
     1021 ggggtttcat catgtttaat gaagtccata gtattcatgg tcatacatta ttgttgatca
     1081 ctaaaccttc tttgcaggcg acagctctct tgcagcacct taaacaatcg ctagcaatta
     1141 caggaaaatt acataatatt caacgttctc tggacgatat ctcttcaggc tctattattc
     1201 ttctggatat gatggaagcg gataaaaagc ttatccatta ttggcaggat actttgagca
     1261 ggaaaaacaa caatatcaaa atattgttgc taaatacgcc tgaagattac ccgtaccgcg
     1321 acattgaaaa ctggcctcat atcaacggcg ttttttatgc catggaggat caagaacgtg
     1381 ttgtcaatgg gttgcaaggc gtcctgcgtg gcgaatgcta ctttacgcaa aagcttgcca
     1441 gttacctgat tacacattct ggtaactatc gttataacag cacggaatca gccctcctta
     1501 ctcatcggga aaaagagatc ctgaataagc tgcgtatcgg cgcgtctaat aacgagatcg
     1561 ctcgttcgtt gttcatcagc gaaaatacgg ttaaaacgca tctttataat cttttcaaga
     1621 agatagccgt aaaaaaccgg acacaagcgg tttcctgggc aaacgataac ctcaggcgat
     1681 aaagccatga aacgttattt acgctggatt gtggcggcag aatttctgtt cgccgcaggg
     1741 aatcttcacg ccgttgaggt agaagtcccg ggattgttaa ctgaccatac tgtttcatct
     1801 attggccatg atttttaccg agcctttagt gataaatggg aaagtgacta tacgggtaac
     1861 ttaacgatta atgaaaggcc cagtgcacga tggggaagct ggatcacaat aacggtcaat
     1921 caggatgtta ttttccagac ttttttattc ccgttgaaaa gagactccga gaaaactgtc
     1981 gtctttgcac tgattcaaac tgaagaagca ctaaatcgtc gccagataaa tcaggcgtta
     2041 ttaagtacgg acgatttggc gcatgatgaa ttctaaataa aaaattgttc ggaggctgca
     2101 atgcgtgtca aacatgcagt agttctactc atgcttattt cgccattaag ttgggctgga
     2161 accatgactt tccagttccg taatccaaac tttggtggta acccaaataa tggcgctttt
     2221 ttattaaata gcgctcaggc ccaaaactct tataaagatc cgagctataa cgatgacttt
     2281 ggtattgaaa caccctcagc gttagataac tttactcagg ccatccagtc acaaatttta
     2341 ggtgggctac tgtcgaatat taataccggt aaaccgggcc gcatggtgac caacgattat
     2401 attgttgata ttgctaaccg cgatggtcaa ttgcagttga acgtgacaga tcgtaaaacc
     2461 ggacaaacct cgaccatcca ggtttcgggt ttacaaaata actcaaccga tttttaagcc
     2521 ccagcttcat aaggaaaata atcatgcagc gcttatttct tttggttgcc gtcatgttac
     2581 tgagcggatg cttaaccgcc ccgcctaaag aagccgccag accgacatta atgcctcgtg
     2641 ctcagagcta caaagatttg acccatctgc cagcgccgac gggtaagatc tttgtttcgg
     2701 tatacaacat tcaggacgaa accgggcaat ttaaacccta cccggcaagt aacttctcca
     2761 ctgctgttcc gcaaagcgcc agggcttaca aaacctgctt aacgagcgca aggttattcg
     2821 tgcggcacaa gaaaacggca cggttgccat taataaccga atcccgctgc aatctttaac
     2881 ggcggcaaat attatggttg aaggttcgat tatcggttat gaaagcaacg ttaaatcggg
     2941 cggtgttggg gcaagatatt ttgggatcgg tgcagacacg caataccagc tcgatcagat
     3001 tgccgtgaac ctgcgtgtcg tcaatgtgag taccggtgag atcctctcct cggtgaacac
     3061 cagtaagacg atactttcct atgaagttca ggccggggtt ttccgcttta ttgactacca
     3121 gcgcttgctg gaaggggaag tgggttacac ctcaaacgaa cccgttatgc tgtgtctgat
     3181 gtcggctatc gaaacagggg tcattttcct gattaatgat ggtatcgacc gtggtctgtg
     3241 ggatttgtaa aataaagcag aacggcagaa tgacattctg gtgaaatacc gccatatgtc
     3301 ggttccaccg gaatcctgac agataaaaat gccggaatta ataatccggc atttactgtt
     3361 taagcgaaag cgtgagtgcc cctcacgctt tatttttgat ttcctgctca cggtgagccg
     3421 tctttgcact acgcgccgcc agccacaggc cgctgttttt cattgcgtaa ccaaatacca
     3481 gcccaagcgc cagcgaaggt aacaccagtt tccagtcacc ctgcccggca aacgtcgcgc
     3541 atgcgcctat aaacgtcccc ggaacaaatg aaagcagcag ctgtttggcc tgaatacaca
     3601 tcagaaacgc gacaataccg gttatgacat aaccgagaat ttccagatgt ggtgccagtg
     3661 cactaccgta aataatgacc atcgcccaca ccacgccact aagcagggtt gcagcggaga
     3721 tcgccagccc tttcagccca ccttgcgggc aagcaaagta ggccgtacag cccaggaagc
     3781 ccgcccagct tagtaagccc agggaaacag ccacccatcc ccagataccg gagagaatgc
     3841 ctgttgtgat tgcaatagag agaagtatgt tcatgccgcg catgatagca gaaaacgcgg
     3901 ccatgaatga gatcgagcac acattttatg caacaacaaa ttatctgttg cagttatgag
     3961 tgacgatgat cacatttact cttcagaatc ttcttccagc tcgtcccaca ttgcactaat
     4021 ggcatcacgg gttagcggtg ccatggtgcg ccagaaaggc gtggttgcat gggcctccac
     4081 tttgccgagg aacgcaccac accagggtaa cagatactca ctgaacagtg tttccagtgc
     4141 ttcgctctca tcttccgttg actgatcttc cagccaggaa gccgcaagca gcaatgtgcc
     4201 gatgtgatcg gctggcgtat ccgccagtag catccctcgt tcggaaagaa aagcgcgcac
     4261 ttccgcttcc gttgcgtcct caacccatgc gctacgatat ggcggcacgg cacattcatc
     4321 gccgataaac aacgcattgt aatcggcaga gacttgcgtc atatcacagc ttttctgtaa
     4381 acgcgtcagt aactcatcct gctccagtgg ccagttcgca gccagtttcc cctcacgaat
     4441 cagagtaaac agcggcacca gtaaaggatc ttgcggttgg cggtaataga gcgaacccag
     4501 cacacgacag aggatagaaa actcgttcat taatatattt ccattactag taattaaagg
     4561 tctgcaaatt ccgcaatcgg tgccataccg cgagattcaa ggaagttcag taagcggcgc
     4621 ggagaaacat tcaaaatgcg ctctagcgga aaatctaccg cgtcgaggat tttaagacac
     4681 tcttcaaatt cccccatggt aaacgcggtg tgagaatccg agcctaatgc cacccaacct
     4741 ccagcatcac gtaccgctgc agctaccgca cgacagttgt cttcactgcc cttacgtgag
     4801 tgtaaaaatg aggaattatt gatttccagc gccacctgat gtttcgcggc tgcttcagca
     4861 acggctttaa catctatttc atatttcgga tttccgggat ggcttattat atgcacattg
     4921 ccgctggcga tagtggagat catcgcttgt gtattggtcg ctttgtcatg tggcgcaaaa
     4981 accggctcat gaaaaccggc aataatcaga tccagcgagt caaacatttt accgctgcag
     5041 tcaatttcac catcaacatt tttaatgtta gcttcaatgc cgcgcaggat ccctacccca
     5101 tcaaccactc gcggccagat acgcatgtta atgaagtgcc agtgatgcgg cgcatcttcc
     5161 atatccgggc catgatcggt gatcgcaaaa agtttaatgc ccttttgttt ggcctgggca
     5221 atgtaatcac tcaatgtgct atatgcatgt gtgctggcaa cggtatgcat atgaaggtcg
     5281 acgggataca taactctctc ctgtattcaa ttcccgccaa ggatagcagg aatcctgatg
     5341 ctttattagt agccacgtgc gcggtcgact tgcccgcaga ccctctcccc tttttcgagc
     5401 tgggcaatag tgcgagaaat gtactccaca gcttcagcgg gacgggtaat cgcggcgaca
     5461 tgtggtgtta tcgtcacgcg tggatgttgc cagagcggac tttcaggcgg taagggttca
     5521 cgattaaaaa catccagcat tgcgccttta actttcccgc tatccagcgc cgcgagcagg
     5581 tcatcttcca caacatgaac accacgcgcc aggttgagga gatacgcgcc atcaggtaat
     5641 ttttcgagta attgttgatt aataatgccg acggtttcag gggtattcgg taacaaatta
     5701 atcaataccc gacattggct cagaaatgca gacagttctt cccgtccggc aaagctttgc
     5761 acgccaggcc acgatttacg ggttcgactc cagcaacgca gcggaaagcg ctaggtttgc
     5821 agactctgag caactttaca gcccagtacg cctgcgccca aaatgccgat ggtaaaatct
     5881 tcccgatgat attcaggcag cggttgccaa tgcgaactat tttgctggat gcgataatcg
     5941 tcaaaacgtc gaaaccaatg cagcacctga ctgacagcat attcctgcat ttgctcgccc
     6001 ataccggtat cttccaggcg aaaaagtgga acagaagggt tcagcatttc agggtgtgcc
     6061 tgtagcttgc tcaaaataga atcaacaccg gccccgagtg cgaacaccgc tttaagatcg
     6121 cgccctgcca gcatttcaac aggaggatgc cagactaaaa cataatcagc agagtcatta
     6181 tctccgcttt tccatgccct gactcttgcc tgaggaatag ctttgcgcag tgcctcaatc
     6241 caccattggg tatcgaacgt tgggtgataa aagatgatat ccatactgac tcccgaaaag
     6301 cgttgtgcga aatttattcg cacttatcgt tatgatctac aaaggccacc agcataacaa
     6361 atccgtggtc ggtggcaaaa aaagcagatt tcgcttatta aaaccacaca ttgattgaat
     6421 gtttgaataa acgcgcgatt ttttcaaaaa gtttgttgac ctcaggtcat gatttcccta
     6481 aattagcgcc cgttccagca agacaggaac gacaatttgg tgaggtgtcc gagtggctga
     6541 aggagcacgc ctggaaagtg tgtatacggc aacgtatcgg gggttcgaat ccccccctca
     6601 ccgccagatt ataagtgcat gttattgttt aacatgcgct tttttatttt tccttccaga
     6661 cagtatgcca tcgggtatgc catctgaaac cggtttatat gccccgcgat catatcttat
     6721 cggacaattt ccgatctttg agcatactgg caaagctata ctgccagcat atcaggatag
     6781 tgttttgcga taatatcatt cagtctgtca accagttctg gttttctgaa cgtgatatgc
     6841 gcggagccct tcttaaagta tctgatgctg aacatctcat cttcatagct ctctttaccc
     6901 tgaacagact gaatgtgatc atccagacga agggtgatgt ttccccggtt gtcggggatt
     6961 ggtttaccgc tgaacaggtg caacattctt tccagatcgg cgagtcggtc agcctgttgt
     7021 ccggtgataa gatgaaatct atggtcaccc ccgtttttgc aacactgatt tttgattaat
     7081 ggttggcttg cttaaatcta tccggcgtct ctgtgggcaa ggatgcccgc gccccgatga
     7141 gttccgcacc tgataatcct aaaaatcccc tcggcttcag tgagccattt catctgttag
     7201 gtttttaatc aggtcgattt gccgttcatg tcatcaataa tcagtcttcg caaaaccaga
     7261 aaggtaactt gtatcttatt gtttaaaata aactaattga gttatatcag catctatatc
     7321 aggcctgata ttcttttttc tgagtagtga ttgcccaggc aattcgagcc agtttatttg
     7381 ccagagcgca ggtaaccaca ttcgaatgct tcttgttgag ttgctcccga acccactcag
     7441 caagttttcc atgcttgcgt tcaagtcgca ttatgaatga gcgggcacac tgaaccagta
     7501 accggcgtaa atttttatca cctcgcttac tgatacccaa cagagtcgat tttcctccag
     7561 tgctatactg ccggggaaca agtcctgtgg aagctgcaaa atccctgctg catgagaact
     7621 gtttcccgtc accaagttgc gacgatagta agctggcagt aatcggacca acaccaggaa
     7681 ttgtcataat gcgctgagcg gtatcatcag cgttgattga ctgacttaat tctgattcca
     7741 gctctgctat ttgctcaaca aggtaaaagt agtgggtatg caacctcacc aaaaggcggc
     7801 tcagatattc ggggatttcg tgttcagcca acacgagaga caaccgtttt attacagcat
     7861 cacctatcgg caggctgatt ccaaattcca gcaagaaacc gtggatctgg ttggttgttt
     7921 tcaccttatc tctgatgagc gattctctga cccggtgtaa cgccctcatg gcctgctggg
     7981 tctcagttct tggctggaca aaacgcattg atggtcgtga agccgcttcg catatggctt
     8041 ctgcatcaac aaaatcattc ttattacttt tgacaaacgg gcgaacgaac tgtggagaaa
     8101 tcagcttagt ttcatgccca aaatcagaga tacagcgagc cataaagtgt gcacccgcac
     8161 aggcttccat tacaacaact gagggcggac atgtcgccag aaactgcatc agtttagtac
     8221 gggtaaattt tttacgcaga agagccttac cagacttgtc ttgacaatga atgtggaagg
     8281 agtgctttcc gagatcgata ccaataagtg tgatgttctg catgatggtc tctccgttaa
     8341 agggatttac cttaacagcg tactgctgtt aaggtggggg tgaccatatc attatcccca
     8401 ccggtcccat ctcaccagat tgttgacgat aattttactg ccaaatttac aggggcaatt
     8461 ggttttgtaa ttccagttca gccctctgaa gacgttgatc acccctcgct caaacacctc
     8521 atccttattc tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg
     8581 aaaattatcg tactccagtg accggtacca tgtatcacgg gcctgcgtat ccattaatga
     8641 cagcatcccg gaacgttgca tcaggtcacg ccagatttcg cgatcaagat tccgggtaat
     8701 ggctttcatt gctgtttcgg gtttctccat cagccagcag ccgcagcgaa aatcctgttt
     8761 catggcccag tccggggcag ttttacctcc gatgctttcc gtcagcaccg agatttcagc
     8821 cagttgtcgg ataagtgtgt cgatttgcaa cagtgtactc tttcgtacag ccagaatgtg
     8881 gcgaatgctg gttgagcaga taacgtccgt atgcccgttg accacttccg gacgggagat
     8941 tgtgatatct gacataatcg atattccttt ttgcagataa atgaaaacgc cagccctgtg
     9001 caggctggcg tcagaggaat gaagtctggt gagtgagctt caccggttct ggtgcagaag
     9061 ctgcagatga cgcagggtac ggacatcctc ccgggcctgt tcgatacagg gcaacagtgc
     9121 tgctgaatcc gttttatcct catcgttgtc aaagataatt cccgatccgc agtcgatatt
     9181 gtcctgcagc cacgcaatca gaatatccag cgctgtttcc gtggttaatg atttcatgtt
     9241 gttcctggtt accagtcgaa agtgggtaaa cccggcaggc attcagcgct ggcatccaga
     9301 tgaatgagac tgacgccata acgccggatg agtgtgacga ccagacgacg gaacgttttt
     9361 gacagcccca ggcgtttaag ccgcagaacc gggtatgacc acgcatcggt acgtaacaga
     9421 tatccgctac ctgtaaaatg aatccattct gattcaccga agtcactggt ctggtgtgac
     9481 agcgagtaca accaggcgtt gtccgtttcc gtgatatgtg cggtactgca gcgtatgccg
     9541 gtaagggtag caaatggtgg cgtgcattct gctgaggtct ggtcagattc atcaacaaca
     9601 cgaagggagt agccgttatc gcagaccatg ttaatcagtt cagcgagctc aataccatcg
     9661 acgtcaacga caatgcgccc tatgttcagt gcctgtacgt taacgctgtc ggcttccagt
     9721 gtcagggcca gtttcatcat ttcgcctccg gatacttacc cagggtaatg ttatttaccg
     9781 ttctgtaatt gtcgcgggtc atcaggccgg tcgcccggcg agcccggagg atatcaatac
     9841 tgtttattaa ctgagagcgg ggacaggtgc tgaatcccgg ctggtcggta cgcaccagcg
     9901 cgtatttttc cacgagaaaa ttaactgcat cacacagtga aatgcctgcc tcaatatgct
     9961 gctcaatcac acgttcatcg acaaacggtg tgtcattcag tgtgaggcca taatgctggt
    10021 ccagcagtcg gatgagcagt gtctgccaga tggtgacggg agacgggcag cgagatacct
    10081 cccgtacatg tgtgtcggat aatgttttca tcttaagttc tctgtaactg gtagtgctaa
    10141 aggtgagagc ttcggggtta tgaggtggtt gcgggtgctg gtgtcggata aacagccaga
    10201 taaacgtaac cacaggagcc gagggtgtcg gcttcgcagg tcagcccttt tgcatacagc
    10261 gtgacggtat gctgatatcg gggattcagt tcaccgctgg tgagcatgag ttccagttgt
    10321 ttcatcagca gcggaaaggc ctggtccaga tggtatgcat ctgcgtcgct gaagcggcct
    10381 ctgataccgg cgcggtcggc aaggtaatac aggcggttac cctcttgcac cagacgagcg
    10441 ccaaaacagg gcctcactgt gcagggaagc ccccaccagg ggcggtcgtg attgtcatcg
    10501 ggatgagtta tcccggagag tttgtctgac acgataaaat cctctctgaa aagtggttaa
    10561 gaatggttta acgatgacag gtcagccggc gcgctgaccg gtttcggtga agatgtatgc
    10621 attgatgaga atggcctcat ccacagcggc gtctgaggtc atctggtcgt actcgtttcc
    10681 cgggtgacgg aacatcagtt cagggtgaca ctcatcagcc ggacatacgg gccaaaactg
    10741 tccttacggc gttctgcaaa cacggcaaga acgccgggaa tatcctgcac ttcacgacca
    10801 gtatacgctt cagcactgcc gtgccagcga tacttaccgg tacagaacgg aaaaagacgg
    10861 gatgcaggat gctgttggtg aatacgcatg gcttcaccac gggtgatgat tctcatgatg
    10921 tgatacctct gaagacagaa gataaaagtg aaaacaggtg tgatgtggtt gtgacgggtt
    10981 aaagcagacc atgttcggca aaggagaaaa cctggttgcc accgactatc agatggtccg
    11041 gcacccggat atccaccagg cccagtgcct gtaccagacg ttcggtgata agccggtctg
    11101 ccttactggg tgtgacttca ccggacgggt gattgtgcgc cagcaccacg gcagcggcat
    11161 tgtggtacag ggcgcgttta atcacttccc ggggatgaac ttccgtacgg ttgatggtgc
    11221 cggtgaagag ggtttcaccg gcaatcagct gattctggtt gttcagatac agcacccgga
    11281 actcttcccg ctccagtccc gccatgttca gaatcagcca ttcccgtgcc gcacgggtgg
    11341 aggtgaaggc cacgccgggt tcatgaagat ggcggtccag ggtttgcagg gcccgcagaa
    11401 tgagactgcg ctcgccgggc gtcatgtttc cgggcagaaa ggaaagctgc tgcattgtcc
    11461 ctgcctccat tcagtcgatg atgcgcataa tggcgctgca ttccggatgc tgcagggcgt
    11521 aatcccgcag ccggtaataa tggaccgtca tggcataaca ctccgtacga caggcatgat
    11581 gactgtacgt catcagacag gcagcaatgc cggcggcttc cgggctcatt tcagcgcggt
    11641 taccgttcat ggcattgaac agtacccatg tttcgtcatc atcgtcatcc ggttcgggtg
    11701 ccataaatgc cccgccgttg ttcagggtgt acagattcca gataccaccg cagtagtctt
    11761 cacacagacg gtccatccag ccgaagacac ggggttccag ggtgacccac tgtggaatga
    11821 ggccaaaatg ctgcggccag aagctgatgc gctgttcatc agggacggga gtggcaacca
    11881 gttgttgcag gccagtctcc ggtgcacagg ccgaagcggt ggtgttctga gacaatgttt
    11941 tcatgtatga aatccttgta atgtgaataa atgagaggga gtaaaggtcg ggagctggca
    12001 acatcacgtt catcactgac cgtgatgacc cggtaatatc agggatgaat gatattcggc
    12061 tgaaattccg gacagccgta cgcgtaaccg ttccggtcac agacggatgg ccagatgcca
    12121 ttgcctgtat actcagtctg cacagactga tttctccggg acggtaaagc agacgggtac
    12181 accaggcgca caggtcatca tcagaaatgg aacgacaccc cggcagcgcc tccggggcgt
    12241 cgggtgattc tggtgcctga atcacgcctt cagggacatg ctgcctcagc gcaggctctc
    12301 cagcagcgtt tctgccatta cccacaatgc gcggttgagc ttaatatcgg tgtcgatgtt
    12361 gtgaatggca cgggtgtgga tacgttttcc ttttgcactg cggccggaaa tcccgccttt
    12421 cagcatattc tcctggatgg tctgatatgc gctccacagg tctttaccgt aatcctccct
    12481 gcgtcgtggt gtcagaatgt cagcggtggt gactggctga tgttcgtcac cataacggta
    12541 agtcagtgcc gcctgtgcca gcgcctggcg tgccggtggc ggcaggacca gcgactgcat
    12601 ggcatcacgc ttctcctcta tccggtcaaa cacgcccacc acctcgtaag ccccttcaat
    12661 aactttctcc accacatttc cccggtgtgg aacacgcaat tctcccagag actgaccgca
    12721 gaccagtcca ttggtacaaa tggcacgaaa atatccgggt aacatctggt agctggatga
    12781 accgtcatga gagttgagca gaataatttc agggacatgc tgaccggtta tctgtccggc
    12841 ccgccgcaga cgcagcatgt gttttgtgta ttcccggcga cccgggtcac gcacacgtgt
    12901 ctggcaggcg aagaatggct gaaagccttc ccgctgcagg ctttccagta cggtgatggt
    12961 gggagtgtac gcatagcgtt tactgcggga agtatgccgg tcttcaccga aaatactggg
    13021 aacatagtgc atcagttctt cgtgtgtcag cggacggtca cggcgtatct ggtttgcagc
    13081 ataaccaaaa cgactggcta gtcgcataat ttgctcctta tcggtggtta agatttactg
    13141 gtgtaataaa tgaaaaagcc acgtctcccg gagaagacgc ggcctgatgg acgaaattaa
    13201 tgaggtttat tgtctgagaa gaccgtcact ggcgaacctt ccggcgtcag tctctgcgtt
    13261 gcggataacc cgacggaaat gagttccctg gtcatcgtcg ataaacacat tgcggtaagc
    13321 tgttgtgatg acaacagcct gctcgcgtga gaaagggact tcgggcaata aacgtgacat
    13381 acaaccagac ctgtatttgg ttccctgaaa atataagtcc ctgtcatcat ggcagagccg
    13441 tatttacttt tgatatcaat aaattaataa cgcgaagatg aaccgcataa cttacgcctt
    13501 taatttgttt tgtttacgta gtccctgacc aggtacgcga attcgtgaac gccagtttca
    13561 ccaaaccaaa tcaggtaacc agggtcgatg ttgtaatagt aaccaacgac tttatgcgtt
    13621 tttgggttat agagtgttac gatatagtcc cagccgcagg cgtgggcttt acaaccgcca
    13681 ccttctaccc aggtcgttcc gttaatgttt acaggatacg caccaccatc aggaccatcc
    13741 atatagccgt cttccccaaa cattccgttt aacccaacag ggtttaatgc tttgtcgata
    13801 gcagctctgt aggtagcgtc accttcgtat aactcgtacg ttgttggaca tgcaccaggc
    13861 tggtcagccc gggcttttga gtcgcattta agtgtctgat ttacagtggc ggcgtttgtg
    13921 tcaggactt
//
LOCUS       DAJTUP010000098        13617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTUP010000098 DAJTUP010000000
VERSION     DAJTUP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.098"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO468_003122"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO468_003122"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR6260142.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO468_003123"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO468_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR6260143.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO468_003124"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO468_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR6260144.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO468_003125"
     CDS             3807..3995
                     /locus_tag="OO468_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR6260145.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO468_003126"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO468_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR6260146.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO468_003127"
     CDS             4756..5115
                     /locus_tag="OO468_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6260147.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO468_003128"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO468_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO468_003129"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO468_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR6260148.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO468_003130"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO468_003130"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR6260149.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO468_003131"
     CDS             10913..11254
                     /locus_tag="OO468_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR6260150.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO468_003132"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO468_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO468_003133"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO468_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR6260151.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         3195 a         3474 c         3715 g         3233 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaa
//
LOCUS       DAJTUP010000099        13372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTUP010000099 DAJTUP010000000
VERSION     DAJTUP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.099"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..2452
                     /locus_tag="OO468_003134"
                     /pseudo
     CDS             1..2452
                     /locus_tag="OO468_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2462..6108
                     /locus_tag="OO468_003135"
                     /pseudo
     CDS             2462..6108
                     /locus_tag="OO468_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6169..6576
                     /locus_tag="OO468_003136"
     CDS             6169..6576
                     /locus_tag="OO468_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260152.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7118..8206
                     /locus_tag="OO468_003137"
     CDS             7118..8206
                     /locus_tag="OO468_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR6260153.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8217..10496
                     /locus_tag="OO468_003138"
     CDS             8217..10496
                     /locus_tag="OO468_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260154.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10489..11625
                     /locus_tag="OO468_003139"
     CDS             10489..11625
                     /locus_tag="OO468_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR6260155.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            11622..>13372
                     /locus_tag="OO468_003140"
     CDS             11622..>13372
                     /locus_tag="OO468_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260156.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPST"
BASE COUNT         3294 a         3527 c         3442 g         3109 t
ORIGIN      
        1 gtgcaaaaag aagagaatag ttggcgcatt atgctaatga cgatgtttat ctcgcaacca
       61 gcactggcag aacaggtcat tccctggctt tcgacacccg cagttgcagt gctgaaatca
      121 tgccagcacc aactgacaca gccctcaaac catgccagcg ccgatctact gccagccata
      181 gtagtctccc caccatggat ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
      241 gcgccattag gcatcgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
      301 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
      361 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
      421 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
      481 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
      541 gctggagaaa gcagtaaaag catggaatgc ccttactcta tctccacata ccgataccga
      601 atacgccatg ttacattttg gactcaaagg tttacctggt ttagttaact caattacacg
      661 ctatccacag gaagccttgc ccatcacgaa ttacttcgca gcgagtgagc tggctcctgc
      721 cgtcgcccgt gccttcaaca aactcaaaac gctacgcgaa aacgcccgca cctggctgtt
      781 gaaatacccg gaacatgccc ttaccggcct gctgcctgcg gcgctcggca aagccggtga
      841 agcacaggat aacgcccgcg ctgccttgcg tatgcttacc gaaaacggtc atcagccatt
      901 actgcaagaa atcgcccggc gttataacca gtcggaagta acagacgcgg tgaacgctct
      961 gcttgcgctc gatcccttag ataatcaccc gacaaaaatc cccactcttc cgacctttta
     1021 tcagccatcg atctggacgc gcccggtatt aaaagcaaac gcccaatcac tgccagataa
     1081 cgccctcctc cacctcggcg aaatgctccg cttccctcag gaagaggctc tgtatccggg
     1141 attattgcag gtgaaagacg cctgtaccgc tgactctctg gctgaatttg cctgggatct
     1201 gtttaccgcc tggcagaccg ctggcgcgcc gtcgaaagag agttgggcgt tcactgcgtt
     1261 aggcgttctc ggtaacgatg acaccgcccg caaactgacg ccattaatac gcgcctggcc
     1321 tggtgaatcc cagcataaac gcgccaccgt tgggttggat attctcgccg ctatcggtag
     1381 tgatatcgcc ctgatgcagc ttaacggcat tgctcagaaa ctgaaattca aagcattaca
     1441 ggagcgggca aaagaaaaga ttgccgacat tgccgagagc cgcgaactca cggtagcgga
     1501 gcttgaagat cggttagcac cggatctcgg tctggatgat aacggttcgt tgctgctgga
     1561 ttttggccca cgccagttca ccgtcagctt tgatgaaacc ttaaaaccgt ttgtgcgtga
     1621 tgtttccggc agccgcctga aagacctgcc aaaaccaaac aaaagcgatt atgagtcaca
     1681 ggcgaacgat gctgttaacc gctacaaatt gctgaaaaaa gatgcgcgta ccgtcgcggc
     1741 ccagcaggta gcaaggcttg aatccgccat gtgcctgcgt cgccgctggt cgccggaaaa
     1801 cttccaactc ttcctggttg tgcatccgct ggttcgccac ttaacccgcc gtctgatttg
     1861 gggcgtttat agcgccgaca accagctact ggcctgtttc cgggtagcgg aagataacag
     1921 ctacagcacc gctgacgatg atcttttcac cctgccggaa ggcgatatct ctatcggcat
     1981 tcctcacgtt ctggaaatat caccgacgga tgctgtcgcc tttggtcagc tttttgccga
     2041 ctacgaactg ctaccaccgt tccgtcagct tgaccgtaac agttacgccc tgacagaagc
     2101 cgagcgcaac gccagcgaac tgacccgctg ggcaggcaga aaatgcccga gcggccgggt
     2161 catggggctg gcaaataaag gctggataaa gggcgaacca caggatggag gctggatgat
     2221 caaacctctc ggtcgctggt cgttaatcat ggaaatcgac gaaggttttg cggtgggtat
     2281 atcaccagcc gaacttagcg ctgagcagct cttaagcaag ctgtggctat gggaaggcaa
     2341 agcggaaagc tatggctggg ggagtaattc aacacaggaa gcgcagttct ccgtactcga
     2401 tgccatcacc gccagcgagc taattaacga tattgaagcc ctgtttgaat aaggaaagag
     2461 catggacaag gaattaccgt ggctggcgga taacgcccaa ctggaactga aatataaaaa
     2521 aggcaaaaca ccactcagtc atcgcaattg gccgggcgaa ccagtgcctg ttatcactga
     2581 aagcatcatt cagacactgg gtgatgaatt gctacaaaaa gctgagaaga aaaaaaacat
     2641 tgtctggcgt tatgaaaatt tttcaccgga gtggcagtcc gccattacgc aggccatcaa
     2701 cttgatcggc gaacacaaac cctcagtccc ggcccgcaca atggcggcac tagcctttat
     2761 cgcgcaaaat gacaaccaac agttgctcga cgaaatcgtc caacaagagg ggctggaata
     2821 tgcgactgag gtggtgattg cacgccagtt tattgcacgg tgttatgaga gtgatcctct
     2881 ggtagtcaca ttgcagtatc agaacgagga ctatggctat ggttatcgct cagaaaccta
     2941 taacgaattc gatctccgac tgcgtaaaca cctctctctg gcagaggaaa gctgctggca
     3001 gcgttgcgcc gacaaactca ttgccgcact gccaggaata accaaagttc gccgcccttt
     3061 tattgcgctg atcctccccg aaaaaccaga aatcgccaat gagttggtaa gccttgaatg
     3121 cccgcggact cattttcatt ctaaggagtg gttaaaagtt gttgctactg accccaaagc
     3181 ggtgagaaaa ctcgaacgct actggagcca ggatatattt agcgatcgag aagccagcta
     3241 catgtcgcat gaaaaccact tcggctacgc ggcctgcgct gcccttttgc gcgaacaagg
     3301 actggcagtc gttccgcgcc tcgcgatata tgcccataaa gaagattgcg gcagtctgct
     3361 ggtacaaatt aaccatccgc aagtcatccg caccttgctg ttagttgctg ataaaaacaa
     3421 acccagcctg caacgtgtgg ctaaatacag taaaaacttc ccccatgcga cactcgccgc
     3481 actggcagaa ctgctggcgt taaaagaacc accagcccgc ccaggttatc caatcatcga
     3541 agacaaaaag ctgcctgcac agcaaaaagc tcgagatgaa tactggcgta cgctgttaca
     3601 aacgctgatg gcatcgcagc cacaactggc agcagaagtg atgccgtggt taagtactca
     3661 agcccgggca gtgctgaata gttatttatc ggcaccgccc aaaccagtta ttgatagcac
     3721 cgataacagc caaatgcctg aaatcctcgt ttcaccacca tggcgtggga gaaagaaaaa
     3781 gacttccata cgtcactttt cgctgcctga tcttacgctt gcaccgcaaa cgtacaggtc
     3841 gccgaataaa cactggacgc cggaacagca aactgcaatt gaccatttta gcgccatgtc
     3901 ttttgacgaa cgtcttgcta cgagaggagc ggatatcttc ttgcgggaat taggttttga
     3961 cgttcatagc ctggaattta ataagcgtct tcttaatgga cagttggatg cctttgatgc
     4021 tcagcaactg caagaataca catcaatagg actattgcgt acttatcacg cgcatcaggc
     4081 caaatctaaa cagtaccggg aagacgccac caatgcatta ctggcacaag atagcattgc
     4141 gttgatggga gggtggcgaa cttttcagca cggattttac tatggtaatg aacagcgttg
     4201 ggggatttgg aatctgtacc ttatcgcgca gatgccgcgc gaaatggcgg tatcgtgctg
     4261 gcaacaaatt gtcgaggctg attttcatta caccggggtg gaatatttgt taagtgtgct
     4321 gggtaccgat gctttgcctg gattaaacac cgcctttgcc cgacatccaa aagagatatt
     4381 tccgctgctg attcatttcg gcgcaacaga actggccctg cccattgccc gcgtctggca
     4441 cctttttgcg ggccagcgta atctggctcg ccagtggatt ttacaatggc cggaacatac
     4501 ggctactgca cttatccctc ttgtcttcac caaatctagc gataaaagcg aagctgcatt
     4561 acttgccctg cgtttacttt acgaacatgg gcatggcgaa tggctacaaa ctgtagccaa
     4621 tcgttggcag cgtacagatc tatggcctgc cctggaacaa ttacttaaac agagtccaat
     4681 agagatttac ccggcacgca ttccaaaagc ccctgatttc tggcatccgc aaatgtggtc
     4741 caggccgcgc cttatcacta ataatcaacc tgttaccgat gacgctctgg aaattatcgg
     4801 cgaaatgctg cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac
     4861 gttctgccag ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca
     4921 agctggtgcc cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga
     4981 aagcacggca cgggatctaa cgacacatat cctcgcctgg ccacaagaag gcaaatctgc
     5041 ccgtgcggtc agtggcatga acatccttac cctgatgaat aatgatatgg cgctgatgca
     5101 gctgcatcat atatcgcaac gggcgaaatc ccgcccctta cgtgataacg cggcggaatt
     5161 tcttcaggta gtcgcagaaa atcgcgggct aagccaggaa gagttagcgg acagattagt
     5221 cccaacactg ggccttaatg atccgcaggc gttgagtttt gattttggtc cccggcagtt
     5281 taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca
     5341 gaaaagtgtt ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc
     5401 ccgactaaaa gggttaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct
     5461 tgaagctgcc ctacgtacca cccgacgctg gtcgctggca gattttcatt ctctgtttgt
     5521 taatcatccc tttacccgtc tggttaccca gcgattaata tggggggtta tccggcaaat
     5581 gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag agggggagtt ctgcaatgcg
     5641 caagatgagc caattgacct gcctgcggac gctctgattg gcattgccca cccgttagaa
     5701 atgacagcag aaatgcgcag tgaatttgca cagctttttg ccgattacga aattatgccg
     5761 ccttttcgcc agttgtcgcg ccgcacggtg ctgctcacac ctgacgagtc aaccagtaac
     5821 agcctgactc gctgggaagg taaatccgct accgttgggc aacttatggg aatgcgatac
     5881 aaaggctggg agtcaggtta tgaggacgca tttgtctatg acctgggtga gtaccggctg
     5941 gtccttaagt tttcacccgg ttttaaccac tacaatgttg atagcaaagc gctaatgagc
     6001 ttccgttctc ttcgagtgta ccgtgacaat aaatccgtca cttttgccga acttgatgtg
     6061 tttgatttga gtgaggcgtt aagcgcacct gacgtcattt tccattaaaa caaagcgggc
     6121 agtgatgcga ctgcccgtta tctacacgac ttaccagcgg ggaaagcgat gatcgtgcaa
     6181 aaagagctgg ttgctattta cgattatgag gtccctgtac ctgaagatcc gttttccttc
     6241 agacttgaga tccataaatg ctctgaatta tttacaggtt ccgtctaccg actggaacgg
     6301 ttccggctac gtccaacatt tcatcaacgt gatcgagagg atgttgaccc gctaataaat
     6361 gatgcgttga tttatataag agatgagttt attgatgagc aaaaattacg aggcgaatca
     6421 cctgaacctg taatagtaat ttttaattgt aaactacaga atatattcaa ccaacaaata
     6481 gaattatata ctctagataa ttcaagtcgc agcacatgca acttgaagta tgacgagtat
     6541 agctttatta aaggggggaa cggtattccc ccttagatgc gtttcaattt gcccgttact
     6601 tcaacaaatc aatcaaccgt aacagagcca ctaatagctc cagtacagca atgagcacgg
     6661 ttaacttttg tgtcagcgtg tccatccgct ctccttatta aggagcacaa caccagcacc
     6721 ttacccttac actgcctgtc gatactggtt tacgtttgtt gagtcgccat gttgcactcc
     6781 ggatcagagc actacctgcg gcaccacccg tccggtagcc aggacttgaa ataggttcga
     6841 tccggcgcaa cttcccaaca gtcaggatta taaatctcat tatcttaatt gcaacactgt
     6901 ccagaggtgg ttataattcg ttcgttggaa cacgatgttg cacaggcagt ggtgttaggc
     6961 ctgaaaatag taaataaaag aacctgcctc accagcagtt ttttttattt actgtgatct
     7021 gctttccaga tatttttcgc tcaaacaact aatgcgccaa acatttattg cgcgtaaaat
     7081 atcgtttatt tcattaatac atttcaggga tgaatatatg tcaccgcaga ataatcatct
     7141 gcagcgtccg cctgctgctg tgttatacgc cgatgaactg gcaaaattaa aacaaaatga
     7201 taacgcacct tgcccgcccg gttggcagtt aagtttgcct gcggcccgtg cttttatcct
     7261 tggcgatagc gcgcaaaata ttagccgtaa agtggtgatt agtccctccg ctgtcgaacg
     7321 tatgttagtc actcttgcca ccggacgcgg tttgatgttg gtgggggaac ctggcaccgc
     7381 aaaatctctc ctgtctgaat tactggcaac ctctattagc ggcgatgccg ggttaaccat
     7441 ccagggcggc gcatctacta ccgaagatca aatcaagtac ggctggaact acgccctgct
     7501 tatcaatcat ggtccatcaa cggaagcgct ggtccctgcc ccgctttacc agggaatgcg
     7561 cgacggcaaa atcgtccgtt ttgaagagat aacgcgtaca ccgctggaag tgcaagactg
     7621 tctgttaggt atgctttctg acagggtgat gacggtgccg gaactcactg gtgaagccag
     7681 tcagctctat gcgcgagaag gctttaatat tattgccact gccaataccc gcgatcgcgg
     7741 cgtcaatgaa atgagtgctg cgctaaagcg tcgcttcgat tttgaaactg tctttccgat
     7801 tatggatttc gcccaggagc tggagcttgt cgccagtgct tcggcgcgtt tgctggccca
     7861 tagcggtatt ccgcataaag tgcctgacgc ggtactggag ttactggtcc gcaccttccg
     7921 cgatctgcgt gccaacggcg aaaagaaaac gtcaatggat acactaacag cgattatgtc
     7981 caccgctgaa gccgtgaacg tggcccacgc cgtaggcgtc cgcgcctggt ttttagcgaa
     8041 tcgcgcaggt gagccagccg acctggtgga ctgcattgcc ggaaccatcg tcaaagataa
     8101 cgaagaagat cgcgctcgtc tgcgccgtta cttcgaacag cgtgtcgcca cacataaaga
     8161 agctcactgg caggcttatt atcaagcccg ccaccgcctg ccgtgaggaa agatgcatga
     8221 gcgagccgtt aattgtcggc atccggcatc atagtccggc ctgcgcccgg ctggtgaaat
     8281 cgttaatcga aagccagcgg ccacgatacg tgttgattga aggccctgct gattttaatg
     8341 accgggtaga cgaactgttt ttagcccacc agcttccggt agctatttac agttattgcc
     8401 agtatcagga cggtgcagcc cccgggcgtg gtgcctggac gccatttgct gaattttcgc
     8461 cggagtggca ggcgctacaa gccgatcgtc gtattcaggc acaaacttac ttcatcgatt
     8521 tgccttgctg ggcgcagagt gaagaagagg acgattcgcc tgatacgcaa gaggaaagcc
     8581 agaccttgct gctgcgtgcc acccgcatgg ataacagcga caccctgtgg gatcacttgt
     8641 tcgaagatga aagccagcaa actgcattac cctctgcact ggcgcactat tttgcccaac
     8701 tgcggggcga ctcccccggc gatgctctaa atcgtcagcg cgaagccttt atggcccgct
     8761 ggattggatg ggcgatgcag caaaataatg gcgacgtgtt agttgtctgc ggtggctggc
     8821 acgctccggc actggcaaaa atgtggcgcg aatgtccgca ggtaattaac acgccagaat
     8881 tgccgtcgct ggcagatgcc gttacaggtt gttatctcac cccctacagt gaaaagcgcc
     8941 ttgatgtgct ggcaggatac ctttcaggaa tgcctgcccc ggtctggcaa aactggtgct
     9001 ggcagtgggg cttacagcag gcgggagaac aactgctaaa aacggttctc acccgtttgc
     9061 gccagcacaa gttgcctgct tcgaccgcgg atatggctgc cgctcatctg catgcgatgg
     9121 cactggcaca gttgcgcggt catacactac cgttacgcac tgactggctg gatgccatag
     9181 caggatcgct gattaaagaa gccctgaatg cgccgttgcc gtggagctat cgcggcgtta
     9241 ttcatcccga taccgatccg attctgctaa cgttgataga cacattagcg ggtgacggat
     9301 tcggtaaact tgccccttct acgccacaac cgcctctgcc aaaagatgtc acttgcgaac
     9361 tggaacgtac cgcaatctct cttccggcgg agcttacctt aaatcgcttt aaccccaatg
     9421 gactagcgca aagtcaggtg ttacatcggc tggcaatact ggagatccca gggattgtac
     9481 gccagcaggg aagcacactg acacttgcag gcaacggtga agaacgctgg aaattaaccc
     9541 gcccgcttag ccagcatgcg gcattgattg aggccgcctg ctttggtgcc acactcctgg
     9601 aagccgcacg ccataaatta gaagccgata tgctggacgc gggtggaatc ggcagcatta
     9661 ccacatgtct tagccaagcg gcgtcagcgg gtctggcgtc cttcagtcaa caattactgg
     9721 agcaactcac attattaatc gcccaggaaa atcaatttgc cgaaatgggc caggcgctgg
     9781 aagtgctata tgccttatgg cggctggatg aaattagcgg tatgcaaggc gcgcagatat
     9841 tacaaacgac gttatgcgcg gctatcgatc gcacgctgtg gctgtgtgaa tccaacggca
     9901 gaccggatga aaaggagttt cacgctcact tgcatagctg gcaagcgttg tgtcatatcc
     9961 tgcgcgatct acatagcggt gtgcaactcc ccggcgtttc gctttctgcg gcggtagcct
    10021 tgctggagcg acacagtcag gcgattcatg tcctggcact ggatcgcggc gcgactcttg
    10081 gtgcgctgat gcgtctggaa catcccaacg ctagtgccga agcggcgctg acgatgctgg
    10141 cgcagttatc cccggcacaa tccggcgagg cgctgcacgg tttgctggca ttagcccgtc
    10201 atcaactggc ctgtcagccg gcatttatcg ccggtttcag cagtcattta aatcaactaa
    10261 gtgatgccga ttttaccaat gccctgcccg atttacgcgc ggcaatggcc tggctaccgc
    10321 cacgagaacg cgggacgctg gcgcatcagg tgcttgagca ttatcaactg gcgcaacttc
    10381 ccgtttcggc actgcaaatg ccgttgcatt gtccaccgca agccattgca catcatcaac
    10441 aactcgaaca gcaggcactg gcatcgctgc aacactgggg agttttccat gtctgaactg
    10501 aacgatcttc tgaccacccg tgagctacaa cgctggcgat taattcttgg cgaagcggca
    10561 gaaacgacgc tttgtgggct ggatgacaac gcccggcaga tagatcacgc gctggagtgg
    10621 ctgtatgggc gcgatcctga acggctccag cgtggtgaac gctctagtgg attaggtggc
    10681 tcaaatctca ccactcctga gtggatcaac agtattcaca cgctgtttcc gcaacaggtg
    10741 attgagcggc tggaaagcga tgccgtactg cgctacggca ttgaagatgt ggtgacaaat
    10801 ctcgacgtgc tggaacgtat gcagccttcc gaaagcctgc tacgcgccgt tttgcacacc
    10861 aaacatctga tgaaccccga agtactggct gccgcccgca ggattgtgcg ccaggttgtg
    10921 gaagaaatta tggcgcgact ggcaaaggaa gttcgtcagg ctttttctgg tgtccgcgat
    10981 cgtcgtcgcc gctcatttat tccactggcg cgaaactttg atttcaaaag tactctgcgc
    11041 gccaacctgc aacactggca cccgcaacac ggcaagttgt atatcgaatt cccccgcttt
    11101 aacagccgca ttaaacgcca aagcgagcaa tggcaactgg tcttactggt tgatcaaagc
    11161 ggatcgatgg tcgattcggt gatccactct gcggtgatgg cggcctgttt atggcagtta
    11221 cccggcattc gtacccatct ggtggcgttt gacacaagcg tcgttgatct cacggcagac
    11281 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11341 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    11401 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    11461 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    11521 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    11581 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    11641 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    11701 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    11761 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    11821 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    11881 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    11941 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12001 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12061 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12121 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12181 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12241 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12301 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12361 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    12421 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagccac tatcatgccg
    12481 gagaatgctt acgtcagctt gcggcattaa acagtcgatt aaattgcgca caagagatgg
    12541 cccggagcga cagtgttggt gaagttcctc ctgtgccgtg gcgcacggtc gttggctctg
    12601 gcattgccgg agaagcaaag cttgatcatc tgcggctggt gtctttaggt atgcgttgct
    12661 ggcaggatat tgagcattat ggtttacgca tctggtttac cgatcccgac accggcagta
    12721 ttttgcacct ttcgcgcagt tggccgcgaa gtgaacagga agactcaccg gcagctacgc
    12781 gtcggctgtt tagttttcag gctggcgcac tggcgggtgg gcaaattgtt tcacaagcag
    12841 caaaacgcag tgccgatggc gagctgctgt tagctacccg caaccgctta agcagcgttg
    12901 taccgctgtc gcctgatgcc tggcaaatgt tgagcgcgcc gttacgccag ccgggcattg
    12961 tggctttgcg ggaatattta cgccagcgtc cccccgcctg catacggcct cttaatcagg
    13021 tcgataactt atttattctg ccggtcgctg agtgtatttc gctcggttgg gatagcagcc
    13081 gccagacgct ggatgcgcag gtaatcagcg gcgaaggtga agataatctg ctgacgttat
    13141 cactgccagc ctcagccagc gcgccttatg ccattgaacg catggcggcg cttttgcaac
    13201 aaacagacga ccccgtgtgt ctggtttctg gctttgtcag ttttgttgat gggcaattga
    13261 cactggaacc acaggtaatg atgacaaaga ctcgtgcctg ggcgctggac gcagaaactg
    13321 cgcctgtggt ggtatcacta ccttctgcca gcgttctgcc tgtgccgtct ac
//
LOCUS       DAJTUP010000100        13248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTUP010000100 DAJTUP010000000
VERSION     DAJTUP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.100"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1505
                     /gene="ldtD"
                     /locus_tag="OO468_003141"
     CDS             <1..1505
                     /gene="ldtD"
                     /locus_tag="OO468_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6260157.1"
                     /translation="FQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMG
                     YLHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQY
                     AAMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPG
                     DDTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQG
                     LGADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQN
                     GNQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLES
                     HGYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIY
                     LHDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVN
                     IRQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            1686..2234
                     /locus_tag="OO468_003142"
     CDS             1686..2234
                     /locus_tag="OO468_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR6260158.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2261..2908
                     /gene="gloC"
                     /locus_tag="OO468_003143"
     CDS             2261..2908
                     /gene="gloC"
                     /locus_tag="OO468_003143"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR6260159.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3131..4321)
                     /gene="aspC"
                     /locus_tag="OO468_003144"
     CDS             complement(3131..4321)
                     /gene="aspC"
                     /locus_tag="OO468_003144"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR6260160.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4506..5594)
                     /gene="ompF"
                     /locus_tag="OO468_003145"
     CDS             complement(4506..5594)
                     /gene="ompF"
                     /locus_tag="OO468_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR6260161.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6195..7595)
                     /gene="asnS"
                     /locus_tag="OO468_003146"
     CDS             complement(6195..7595)
                     /gene="asnS"
                     /locus_tag="OO468_003146"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR6260162.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(7764..8966)
                     /gene="pncB"
                     /locus_tag="OO468_003147"
     CDS             complement(7764..8966)
                     /gene="pncB"
                     /locus_tag="OO468_003147"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR6260163.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9232..11847
                     /gene="pepN"
                     /locus_tag="OO468_003148"
     CDS             9232..11847
                     /gene="pepN"
                     /locus_tag="OO468_003148"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR6260164.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12054..12821)
                     /gene="ssuB"
                     /locus_tag="OO468_003149"
     CDS             complement(12054..12821)
                     /gene="ssuB"
                     /locus_tag="OO468_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR6260165.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(12818..>13248)
                     /locus_tag="OO468_003150"
     CDS             complement(12818..>13248)
                     /locus_tag="OO468_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6260166.1"
                     /translation="IDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYGLSG
                     IPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQTDV
                     VVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3361 a         3379 c         3342 g         3166 t
ORIGIN      
        1 ccttccagca acagctggca gaggtggcga ttgctggttt ccagccgcag tttaataaat
       61 gggtagagtt actgaccgat cctggtgtta acgggatggc acgtgacgtg gtgctctctg
      121 atgcgatgat gggctatctc catttcattg caaatattcc gatcaaaggc actcgctggc
      181 tatatagcag taaaccttat gcgctttcaa cgccgccgct ttcggtgatt aaccaatggc
      241 agctagcgct ggataaaggt caattgccta cgtttgttgc aggactggca ccgcagcatc
      301 cgcaatatgc ggcgatgcat gaatcgttac tggtcttact cagtgacacc aaaccgtggc
      361 cccaactgac cggcaaagca acgttgcgcc cagggcagtg gagtaacgac gtaccggcgt
      421 tgcgcgaaat attgcaacgc acaggcatgt tggacggggg gccgaaaatt actctacctg
      481 gcgatgacac gccaactgac gcggtagtca gcccatccgc tgttactgtt gaaacagcag
      541 aaactaagcc gatggacaag caaacgacgt ctcgtagtaa acctgcgcct gccgttcgtg
      601 ccgcctacga taatgaactg gtggaagccg ttaaacgttt tcaggcatgg caaggattgg
      661 gagcagatgg tgctattggc ccggcaacgc gtgactggtt aaacgtaacg cccgcccagc
      721 gtgctggcgt gttggcactc aacatccagc gattgcgctt gctgccaaca gagctttcta
      781 ccgggatcat ggttaacatt ccagcctatt cgctgctcta ctatcagaac ggcaatcagg
      841 tgctggattc gcgagtcatt gtcggtcgcc ccgatcgcaa aacgccgatg atgagcagtg
      901 cacttaacaa cgtagtggta aacccgccgt ggaacgtacc accaactctg gcacgcaaag
      961 atattctgcc gaaagtgcgc aacgatccgg gatatctcga aagccatggc tatacggtga
     1021 tgcgcggctg gaacagcaga gaagcgattg acccatggga ggttgactgg tctacaatca
     1081 cggcctcgaa tttaccgttt cgcttccagc aggctccagg cccacggaac tcgctggggc
     1141 gctataaatt caatatgccg agttcagagg ccatttattt gcatgacacg ccgaaccaca
     1201 atctgttcaa gcgtgataca cgcgcattga gctcaggctg tgtacgagtg aataaagctt
     1261 ccgatctggc gaatatgctg ttgcaggatg caggatggaa tgacaaacgt atttctgatg
     1321 cgctgaagca gggtgataca cgttacgtca atattcggca gtcgattccg gtgaatctct
     1381 actacctgac ggcctttgtt ggtgcagatg atcgtaccca gtatcgtaca gatatttaca
     1441 attatgatct gcctgcgcga tccagctcgc aaatcgtatc gaaagcggaa caattaatca
     1501 ggtaaatgaa gtagttcggg gaaatcaatt gtcgtaataa ttagcatgat tgggggcgat
     1561 tctctgcagc ccccgtcact gctggggttg agtcatcttg acgtctgctt tacgggcggt
     1621 taaggtgcct cttgtgcgcc agaagtgcat ataaacgata acattgagct gtagacttga
     1681 ttatcatgga caaattcgac gctaatcgcc gcaaattgct ggcgcttggt ggcgttgcac
     1741 tcggtgccgc catcctgccg acccctgcgt ttgcaacact ctctacccca cgcccgcgca
     1801 ttttgacact caataatctt cataccggag agtcaatcaa agcggagttt ttcgatggca
     1861 gaggctatat tcaggaagaa ttggcaaaac ttaaccattt tttccgcgat taccgcgcga
     1921 acaaaataaa gtccatcgac ccaggattat tcgaccagtt gtatcgcctg caagggttgt
     1981 taggcacgcg caaaccggtg caactcattt ccggttatcg ttccattgat accaacaatg
     2041 aactacgcgc ccgcagccgt ggagtagcga agaaaagcta tcacactaaa ggccaggcga
     2101 tggatttcca tattgaaggt atcgcgttaa gcaatattcg caaagccgcg ttatctatgc
     2161 gcgcaggtgg tgtaggatat tacccacgta gtaactttgt gcatattgat accgggccag
     2221 cacggcactg gtagtaatcg cttaacgaaa caggggcagt atgaactatc gtattattcc
     2281 ggtcaccgca ttctcccaga actgttcatt gatctggtgt gaacaaaccc gcctggccgc
     2341 actggtcgat cctggcggcg atgcggaaaa aatcaaacag gaagttgatg acagcggcct
     2401 gacactgatg cagatcctgc tgacgcatgg tcatctggac cacgttggcg cagcggcgga
     2461 actggcgcaa cattacggcg tgccggtttt tggcccggaa aaagaagatg agttctggct
     2521 gcaaggcttg cctgcgcaaa gtcgtatgtt tggtctggaa gagtgccagc cgctgacgcc
     2581 agatcgttgg ctgaacgaag gcgataccat cagcataggg aatgtgactt tacaggtgtt
     2641 acattgccct gggcatacgc cgggtcatgt cgtgtttttt gatgatcggg caaagctgct
     2701 gatttctggc gatgttattt tcaaaggcgg agtagggcgc agtgacttcc cgcgtggcga
     2761 tcataatcaa ctgatttctt caatcaaaga taaattgctg ccactggggg atgacgtgac
     2821 atttattccg ggtcacggac cattatctac acttggttat gagcgcctgc acaatccctt
     2881 cctgcaagac gaaatgcccg tctggtaaag gcgcataaaa aagtccactt ttaatgcggg
     2941 cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg
     3001 gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag
     3061 cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg
     3121 tttttaacgc ttacagcact gccacaatcg attcgcacag cggagccatg ttatctggtg
     3181 tcatcccggc cacatttacg cgaccagaag caacagcata tacgccaaac tcttcgcgca
     3241 gacgcagcac ttgttctttt gtcagaccac tgaaggagaa catgccgttc tgtttgatga
     3301 taaagctgaa gtcgcggttt gcaccttttt cctgcagcgt attgacgaac aactgacgca
     3361 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaatgcat
     3421 cgctgctcag gatggtggca acaacagaag cgccgtgtgc tggagggtta gagtagttag
     3481 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac gatttcactg tcggcagcaa
     3541 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
     3601 caacaatcag ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca
     3661 gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg
     3721 agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
     3781 agcagccatg gaacagcact acgtcgccag cctgagcttc gttcagactg ttaatcagtg
     3841 catcgaagtc aagggtgtga ttttccgcat cataataagc gtattcacga acttccagac
     3901 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacacacgct
     3961 taacgctggt attttttgcc aggaagtcgg cagccacgcg tagtgcgcca gtgccacccg
     4021 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
     4081 gttcctgagt gcagcgacca aattcaagga tgccgtcaat gccgaggtaa tttttggtgg
     4141 tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
     4201 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
     4261 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
     4321 tgacgaggtt ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta
     4381 caaaattcca actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
     4441 tggaaggcat aaaaaaaaca ggaccaacgt cctgtttttt cggcatttaa caaagaggtg
     4501 tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt
     4561 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
     4621 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
     4681 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
     4741 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat
     4801 ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
     4861 ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt
     4921 tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct
     4981 tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca
     5041 cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag
     5101 ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat
     5161 gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca
     5221 ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc
     5281 agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta
     5341 tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca
     5401 agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa
     5461 aaataatgca gaccaacagt tttaccgtac agatctactt tgttgccatc tttgttatag
     5521 atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata
     5581 ttgcgcttca tcattattta ttaccctcat ggttttttta tgacacctgc cactgccgtc
     5641 aataagttct gtcaataaaa atttacggaa ctattgatga gagtttggtg tctttatgtg
     5701 tctgcaggca tctttccatt caaactaacg tttcgctacc gtgaaagtgc tacaaagata
     5761 aagatttagt ttcaaaaaga aaaaatatgt aaccaaaagt aaaaattaag gagctttgtg
     5821 aacaccgtca tatttccata ggaacgtgat gatatttaca gcaattttta tctatttata
     5881 tgatttactt atatttaaat taactaaacg gaaattttgt ttctgatgga aactttatcg
     5941 acctggcaca aaatcctctt tcagacatcc agaatgccaa aaaatagtat gaaaattgtg
     6001 ctatcagcta agaaaaaact aatccgcaat aagagtacgg gttatttttt gtgcgtaaat
     6061 gttgcaatgt aatattccgt aacaggatga tcgttatggc gggggtgcaa atagattgct
     6121 tgacttaaca aacagataac ttgacagaaa ggataaaaaa gagccagcag gcgctggctc
     6181 tttgaggaag aaaatcagaa gctggcgttg cgcggagtac gtgggaacgg aatcacatca
     6241 cgcacgtttt gcacgccagt tacgtaagca atcaggcgtt caaaaccaag accgaaacct
     6301 gaatgcggaa cagtaccgta gcgacgcaga tcgcgatacc accagtaatc ttctttattc
     6361 aggcccattt ccagcatacg ctcgtccaga acgtccagac gttcttcacg ctgggagcca
     6421 ccaatgatct caccgatgcc cggagccaga acgtccatag ccgcaacggt tttaccgtct
     6481 tcgttgaggc gcatatagaa cgctttaata tctttcggat agtttttaac cactaccggt
     6541 gctttaaagt gttcttccgc cagataacgc tcatgctcag aagagagatc gacgccccag
     6601 taaaccgggt tttcaaactt cctgccgcag ttttcgagaa tggtcactgc gtcggtgtaa
     6661 tccacctgcg cgaaatcggc ttcaatgaag cgttccagac gtgaaacggc atctttatct
     6721 acgcgttcag cgaagaattt catgtcgtct gcgcgttctt cgagaaccgc tttgaagaca
     6781 tatttcagca tggcttcagc cagacccgcg atatcgttca ggttagcaaa cgccacttcc
     6841 ggctccagca tccagaattc cgccaggtga cggctggtgt tggagttttc agcacggaaa
     6901 gtcgggccga aggtataaat tttggataat gcgcaagcgt aggtttcgcc gttcaactgg
     6961 ccagatacgg tcaggaaaga ctctttacca aagaagtctt tgtcgaaatc cactttgccc
     7021 tgatcgttac gcggcaggtt ttccagatcc agcgtagaaa cgcggaacat ttcgcctgca
     7081 ccttcggtat cagatgcggt aatcagtggc gttgaaaccc agaagaatcc ctgctcgtta
     7141 aagaagcgat gcagcgcctg cgccagcgta tggcgaacgc gcgcgacggc accaatcagg
     7201 tttgtgcgcg gacgcaggtg agcgacttca cgcagatact caatgctgtg gcgttttgcc
     7261 gccatcgggt aagtgtctgg atcttcaacc caaccagcaa cttcaacctt gctggcctga
     7321 atttcaaatt gttgcccctg gcccggcgat gccacgactt tacccgtcac aatgaccgag
     7381 cagccggtgg tcagacgcag gacgtcttca ttgtaattgg gcagagaatt attgatgaca
     7441 gcctgtacag gatcaaagca ggaaccgtca taaacggcga ggaaggagat gccagctttt
     7501 gaatctcggc gggtacgtac ccatccgcgt acggtgactt cgctgtcaac ggctacgcgg
     7561 ccctggagta cgtcggctac aggcacaacg ctcataatat tctctctgtt aatagtcgga
     7621 aaaaataaac acttgtccac ccgtaaatgg gggggatacc tatgttacct ggcatctgca
     7681 atcagacaag cagaattcgc aaatgcagcg aaagatttcg gaattaaagg taaaaaaggg
     7741 agccgattag ctccctgatg atattaactg gcttttttaa tatgcggaag gtcgaacgct
     7801 ttgcgcagcg cccgaacaaa cgctttatca tggcagatag ttttgccagg gctgtcagaa
     7861 agtttcgcca ccggtttacc gttacactct accaacttaa tcacaatatt caggggtttt
     7921 acctggggga tatcgcaggt caggcgagtc ccaataccaa aacttaattg cacgcgggaa
     7981 gagaagtggc ggtatagctc aaccgctttg cgtaaatcca gattgtcaga gaaaaccagc
     8041 gttttactct gtggatcaat tcccagcttt tcataatgtg caatggcttt ttcaccccat
     8101 tcaaccgggt cgccagagtc atgacgcagg ccctgatacc gactagcgaa ctcgacaccg
     8161 aaatcacgca ggaaagcatc catagtgatg cagtcggtta atgcaatgcc aagttggtcg
     8221 ggatactctt ccagccaggc agcaagtgca gctcgctggc tgttggctag atccgggctg
     8281 atttgctgat gtgcctggaa ccattcgtgt gcctgtgttc ccatcggcgt gagggaaagc
     8341 cgacgcgcca gatcgtagtt gctggtgccc acgaaccagg attcctgttg cagacgctta
     8401 acgatggttt cttgtacttc gcgagaaaaa cggcgacggg tgccaaaatc catcagatgg
     8461 aagcgcgaca tatcaagacc ggcggttaac gccgagaagt cgactaattt gctttccagc
     8521 gtgtcgaggg cttgcgcaac gtcggcctgc ggtgagcgat agcgatgtac catttcactg
     8581 ataaccgcca gcaaaggaac ttcccagagg atgacttcac gccacggacc gcttaagcga
     8641 atatccagct tgccattatc gttggacacg gtgacttgtt ccgggttaaa gcggaactcg
     8701 cgtaaccagt taagatagtc ggccttaaag aaaggcaggg cagagagcca ctgatattca
     8761 tcatcctgca ggcgcaggtg ctgcatcgcc tgaatctgtt cacgaatagc atcggcataa
     8821 atacccagca gatcgtcacc tcggcaacga aactccgccg cgacatgcac atcgtaatag
     8881 tgatgaaaca cggcttgctg catatgcaac ttataagcat ctgtatccag caacgagtgc
     8941 agaacaggag aagcgaattg tgtcataggt gcgcagtagc gtcctcgtac aggagcgttt
     9001 agtacaataa acatcttcag gaactgctgg agtatacctt gtttcgcaat ttattgaacc
     9061 ccgatcacac catatgccac ctttctggtc gatggcatta cgcggtgtat gttataaaaa
     9121 tgtagcaata aaggcgtttg tacctgaaaa gatgaagatt ctgcatagcg cgatttacgc
     9181 aacaggaata gactgaacac cagactctat aaaagatgct aaaggttatt tatgactcaa
     9241 cagccacaag ccaaataccg tcacgattat cgtgcgccgg attaccagat tactgatatt
     9301 gacttgacct ttgacctcga cgcgcaaaag acggtcgtta ccgcggtcag ccaggctgtc
     9361 cgtcatggtg catcagatgc tccgcttcgt ctcaacggcg aagacctcaa actggtttct
     9421 gttcatatta atgatgagcc gtggaccgcc tggaaagaag aagaagaggg cgcactggtc
     9481 atcagtaatt tgccggagcg ttttacgctt aagatcatta atgaaataag cctggcggcg
     9541 aatacggcgc tggaagggct ttatcagtca ggcgatgcgc tttgcaccca gtgtgaagcc
     9601 gaaggtttcc gccatattac gtattatctc gaccgcccgg acgtgctggc gcgttttacc
     9661 accaaaatta ttgccgataa aatcaaatat cccttcctgc tttccaatgg taaccgcgtt
     9721 gcgcaaggcg aactcgaaaa cggacgccat tgggtacagt ggcaggaccc gttcccgaaa
     9781 ccgtgctacc tgtttgcgct ggtggcaggc gactttgatg tactgcgcga tacctttacc
     9841 acgcgttctg gtcgcgaagt agcactggag ctgtacgtcg atcgcggcaa ccttgatcgc
     9901 gcgccgtggg cgatgacctc gctgaaaaac tccatgaaat gggatgaaga acgctttggc
     9961 ctggagtatg acctcgacat ctatatgatc gtcgcggtgg atttcttcaa tatgggcgca
    10021 atggagaata agggtctgaa tatctttaac tccaaatatg tgctggcccg caccgacacc
    10081 gccaccgaca aagattacct cgatattgaa cgcgttatcg gccatgaata tttccataac
    10141 tggaccggta accgagtgac ctgccgcgac tggttccagc tcagcctgaa agaaggttta
    10201 accgtcttcc gcgatcagga attcagctct gaccttggtt cccgcgcagt taaccgcatc
    10261 aataatgtgc gcaccatgcg cggattgcag tttgcggaag acgccagccc gatggcgcac
    10321 ccaatccgcc cggacatggt cattgagatg aacaacttct acaccctgac cgtttacgag
    10381 aagggcgcgg aagtgatccg tatgatccac accctgctgg gcgaagaaaa cttccagaaa
    10441 gggatgcaac tctatttcga gcgtcatgat ggtagcgcag cgacctgtga cgactttgtg
    10501 caggcgatgg aagatgcgtc gaatgttgat ctctcccatt tccgccgttg gtacagccag
    10561 tccggtacgc cggttgtgac cgtcaaagac gactacaatc cggaaactga gcagtacacc
    10621 ctgaccatca gccagcgcac gccagccacg ccggatcagg cagaaaaaca gccgctgcat
    10681 attccgtttg ccatcgaatt gtatgacaac gaaggtaaag tgatcccgtt gcagaaaggc
    10741 ggtcatccgg tgaattccgt gctgaacgtc acccaggcgg aacagacctt tgtttttgat
    10801 aatgtctact tccagccggt gcctgcgctg ctgtgcgaat tctctgcgcc agtgaaactg
    10861 gaatataagt ggagcgatca gcaactgacc ttcctgatgc gtcatgcgcg taatggtttc
    10921 tcccgttggg atgcggcgca aagtttgctg gcaacctaca tcaagctgaa cgtcgcccgt
    10981 catcagcaag ggcagccgct gtctctgccg gtacatgtgg ctgacgcttt ccgcgcggtg
    11041 ctgctcgatg agaagattga tccggcgctg gcggcagaaa tcctgacgct gccttctgtc
    11101 aatgaaatgg ctgaactgtt cgatatcata gacccgattg ctattgccga agtacgcgaa
    11161 gcactcactc gtactctggc gactgaactg gcggatgagc tgctggctat ttacaacgcg
    11221 aattaccaga gcgagtaccg tgttgagcat gaagatattg caaaacgcac tctgcgtaat
    11281 gcctgcctgc gcttccttgc ttttggtgaa acgcatctgg ctgacgtgct ggtgagcaag
    11341 caataccatg aagcaaacaa tatgaccgat gcgctggcag cgctttctgc ggcggttgct
    11401 gcacagctgc cttgccgtga cgcgctgatg caggagtacg acgataagtg gcatcaggac
    11461 ggtctggtga tggataaatg gtttatcctg caagccacca gcccggcggc gaatgtgctg
    11521 gagacggtgc gtggtctgtt gcagcatcgc tcatttacca tgagcaaccc gaaccgtatt
    11581 cgttcgttga ttggtgcgtt tgcgggcagc aacccggcag cgttccatgc cgaagatggc
    11641 agcggttacc agttcctggt ggaaatgctt accgacctca acagccgtaa cccgcaggta
    11701 gcttcacgtc tgattgaacc gctgattcgc ctgaaacgtt atgatgccaa acgtcaggag
    11761 aaaatgcgcg cggcgctgga acagttgaaa gggctggaaa atctctctgg cgatctttac
    11821 gagaagataa ctaaagcact ggcttgatac ttttgagcca aaatgcctga tgcgacgctt
    11881 gacgcgtctt atcaggccta caactccgtt gaaattgctg gttttgtagg ctggataagg
    11941 cgttcacgcc gcatccggca gccgtgccca actgcctgat gcgatgcttg acgtgtctta
    12001 tcaggcctac aagtccgttg aaaatgctgg ttttgtgcgc cacatccggc atattaacca
    12061 tgacgccgaa tcagttgttc agaatgccca cgccgcatta cccgttgcaa tacttccgct
    12121 tccagctccg ctaatcgcac cgaccctaat cgacgcggac gggggatatc cactgtcaaa
    12181 tccagactaa tttttccctc ttcaattaac aacacccggt cagccattgc taccgcttcg
    12241 ctcacatcat gcgtcaccaa cagtacggta aagccgtgct gctgccaaag tgacacaatc
    12301 aaatcctgca tctcgagtcg cgttaaggcg tccagtgccc cgagcggttc atcaagcaac
    12361 aataaacccg gtcgatgaat caacgctctt gccagcgcca cacgctgttt ctgcccgcca
    12421 gaaagtgcgg caggccattc cccggcgcga ttctccagtc ctgccgcagc caacgcccga
    12481 cgcgcggcat cgcgccactg acctttaagg cctaacccaa cgttatcaat caccgatttc
    12541 cacggcagca gacgcgcatc ctgaaacatc attcgcgtat catcctgaat ttcagccagc
    12601 ggtgtggtgc ccgctaacac atcgcctgcg gttggcgttt ccagaccggc cagcaggtgc
    12661 agcagggtac ttttaccacc accgctgcgg cccaccaccg ccacaaactg acctgccgga
    12721 atatgtaaat ccagttggtt caggacgata ttttccgcgt aatgtttgct tactgcattg
    12781 agcaacaatg gtgtgccctg gttcagacga gcagtattca tatcgtggcc tccttcaaat
    12841 gataagccgg gttccagcgc aaccagaggc gctctaacag ctgcgcgctg acgtcagcaa
    12901 gtttgccgag cagggcgtaa agaataatag cgactaccac cacgtctgtt tgcaaaaact
    12961 ctcgcgcatt catcgccaga taaccaatgc cggaattggc agaaatggtt tcggcaacaa
    13021 tcagcgtcag ccacatcagg cccaacgcaa aacgcacgcc gaccataatt gagggcaggg
    13081 catcgggcag gatcacatgg ataaacagcg gtatgccgga taatccatag ctacgcgcca
    13141 tctccaccag cccgcgatcg atattacgga tgccatgcca ggtgttgata taaatgggga
    13201 acagcgtacc gagcgccacc agaaagattt tcgcggattc atcaatgc
//
LOCUS       DAJTUP010000101        13159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTUP010000101 DAJTUP010000000
VERSION     DAJTUP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.101"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(18..1000)
                     /gene="kdgT"
                     /locus_tag="OO468_003151"
                     /pseudo
     CDS             complement(18..1000)
                     /gene="kdgT"
                     /locus_tag="OO468_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OO468_003152"
     CDS             complement(1260..1880)
                     /gene="sodA"
                     /locus_tag="OO468_003152"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR6260167.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2165..3199
                     /gene="rhaT"
                     /locus_tag="OO468_003153"
                     /pseudo
     CDS             2165..3199
                     /gene="rhaT"
                     /locus_tag="OO468_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OO468_003154"
     CDS             complement(3196..4062)
                     /gene="rhaR"
                     /locus_tag="OO468_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR6260168.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OO468_003155"
     CDS             complement(4118..4954)
                     /gene="rhaS"
                     /locus_tag="OO468_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR6260169.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5242..6711
                     /gene="rhaB"
                     /locus_tag="OO468_003156"
     CDS             5242..6711
                     /gene="rhaB"
                     /locus_tag="OO468_003156"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR6260170.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6708..7967
                     /gene="rhaA"
                     /locus_tag="OO468_003157"
     CDS             6708..7967
                     /gene="rhaA"
                     /locus_tag="OO468_003157"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR6260171.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8209..9033
                     /gene="rhaD"
                     /locus_tag="OO468_003158"
                     /pseudo
     CDS             8209..9033
                     /gene="rhaD"
                     /locus_tag="OO468_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9043..9357
                     /gene="rhaM"
                     /locus_tag="OO468_003159"
     CDS             9043..9357
                     /gene="rhaM"
                     /locus_tag="OO468_003159"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR6260172.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9658..10104
                     /locus_tag="OO468_003160"
     CDS             9658..10104
                     /locus_tag="OO468_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR6260173.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10115..11566
                     /locus_tag="OO468_003161"
     CDS             10115..11566
                     /locus_tag="OO468_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR6260174.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11556..12626
                     /locus_tag="OO468_003162"
     CDS             11556..12626
                     /locus_tag="OO468_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR6260175.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12626..>13159
                     /locus_tag="OO468_003163"
     CDS             12626..>13159
                     /locus_tag="OO468_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR6260176.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQH"
BASE COUNT         3141 a         3431 c         3469 g         3118 t
ORIGIN      
        1 ggattaagga agcaggttta tttcaccgta cctaaaattt cgattttcgc tgctctggct
       61 ttgactttac gtgaccagat agaggtaaga attggcacca gaatcgaagt cacaatgacc
      121 gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt taaacgcagg caccatttct
      181 gcaatcagca caggtgtcgc taccgcggcc cctgcggaac tggaagcggc aattccggca
      241 gtgccatcgc caccgccaat caatttatcg gcgataatca acggaatacc ggtcacgata
      301 attactgcca cacccaacag gatccccagc aaaccagtct gggcaatcac agtcaaatca
      361 atggtattgc ccagcgcgaa ggcaaagaat ggaatcagcg tttgcaccgc tttgctgaaa
      421 aattctcgta attcagggtc aagattcccc agggcaaagc ccaccaggaa cggtaatact
      481 gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg cagtgcccag aataatcatc
      541 gtcatgagcg gcccggactc caacgacatc aacacaaatg cccagcttct tctttcgtac
      601 cgtactgctg catgatggaa gcgtaaagtc cgccgttggt catatccatc gccgccacca
      661 gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc
      721 gcgaggcaat tgccgcaacc acccacgcga cggcaatttt agttaccacc agcgtaccgg
      781 atttacgcag taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc
      841 acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt
      901 tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca
      961 tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata
     1021 tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata
     1081 gacattcatt taatcaataa aagtgatcaa aatcacatca attgaaacgc tgttttattt
     1141 ttataggacc attgttttta ttttggaatt gatcacaaaa aaaccaccgc cgttggcgat
     1201 ggttcatttt ttaagctgat atgcggccta tacgcctcat tgcagcaggc ggcaaataat
     1261 tattttttcg ccgcaaaacg tgccgctgct tcgtcccagt tcaccacgtt ccagaactct
     1321 ttgatgtagt ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc
     1381 aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc
     1441 tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg
     1501 gaaccaaagc gggaagctgc cgctttttca aattctgctt tgaagttatc aacggagccg
     1561 aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct
     1621 ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg
     1681 tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct
     1741 ggcaggcttt ccagcgccgc gttggcgttg tttacgtagg tctgatggtg tttggtgtgg
     1801 tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg
     1861 gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc
     1921 gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg
     1981 tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca
     2041 cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac
     2101 agcacgattt ttacactcat ctcgtcggag atgtgaagcg acgaaaaatg atgaggataa
     2161 gaagatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc
     2221 agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc
     2281 agtcggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc
     2341 ggatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt
     2401 cggcgctatg tgggggatcg gtaatatcaa ctatggcctg accatgcgtt atctcggcat
     2461 gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc
     2521 aattatcaac ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttact
     2581 cggcgttctg gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga
     2641 gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt
     2701 gatgtgcggc attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca
     2761 tgaagccgct gccgcacttg gcgtcgatcc actgtatgtt gctctgccaa gctatgttgt
     2821 catcatgggc ggcggcgcga tcattaacct cggtttctgt tttattcgtc tggcaaaagt
     2881 gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt
     2941 gttactctcg gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctaggg
     3001 ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta
     3061 tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaata caggacgccg
     3121 tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcta acatcgtcgg
     3181 catcggcatg gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat
     3241 cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt
     3301 cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct
     3361 gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa
     3421 aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag
     3481 gctagccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa
     3541 cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc
     3601 agccatttcg ttagcaaacg gcacatgctg actactttca tgctcaagct gaccgataac
     3661 ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc
     3721 gctaaatccc ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata
     3781 aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg
     3841 aatataaaag agatcgccac gggtaatgcg ataaggacga tcgttgagta catgcagacc
     3901 attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaagacatc
     3961 ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt
     4021 gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt
     4081 cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaag ccatcccgtc
     4141 cctggcgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt
     4201 tactgtcgct gaatccacag cgataggcga tgtcagtgac gctggcctcg ctgtggcgta
     4261 gtagatgtcg ggctttcatc agtcgcaggc ggttcaggta tcgctgaggc gtcagtcccg
     4321 tttgctgctt aagctgccga tgtagcgtac gcagtgaaag agaaaattga tccgccacgg
     4381 catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac
     4441 gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt
     4501 gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt
     4561 ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtgag
     4621 acggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa
     4681 atcgatccgg cgagcgatac agcacattgg tcagacacag attatcggta tgttcataca
     4741 gatgccgatc atgatcgcgt acgaaacaga ccgtgccacc ggtgatggta tagggctgcc
     4801 cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat
     4861 gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttacaag
     4921 acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg
     4981 gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa
     5041 aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc
     5101 accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc
     5161 cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat
     5221 gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca
     5281 tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc
     5341 gaaatccatc gttttaacaa tgggctgcat agccaaaacg gttatgtcac ctgggatgtg
     5401 gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt
     5461 atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt
     5521 cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatgccgcag
     5581 gcacaacagc aactcggcaa acgcgatatt tatcagcgta gcggcatcca gtttctaccc
     5641 ttcaatacgc tttatcagtt gcgtgcgctg acggagcaac aacctgaact tattccacac
     5701 attgctcacg ctctgctgat gccggattac ttcagttatc gcctgaccgg caagatgaac
     5761 tgggaatata ccaatgccac caccacacaa ctggtcaata tcaatagcga cgactgggac
     5821 gagtcgctac tggcgtggag cggggccaac aaagcctggt ttggtcgccc gacgcatccg
     5881 ggtaatgtca ttgggcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt
     5941 gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct
     6001 tatctctctt ctggcacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat
     6061 gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg
     6121 ctgaaaaata ttatgggctt atggctgctt cagcgagtgc ttcaggaacg gcaaatcaac
     6181 gatctcccgg cgcttatcgc cgcgacacag gcacttccgg cctgccgctt catcatcaat
     6241 cccaatgacg atcgctttat taatcctgac gagatgtgca gcgaaattca ggctgcgtgt
     6301 cgggaaacgg cgcaaccgat cccgggaagt gatgctgaac tggcgcgctg tattttcgac
     6361 agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctgcg cggtgaagat
     6421 ttctcgcaac tgcatattgt cggcggcggc tgccagaaca cgctgctcaa ccagctatgc
     6481 gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcaac gctcggcaat
     6541 atcggcatcg agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc
     6601 gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcccac
     6661 tatgtggcgc agattcactc tacacgacag acaaaggagc tttgcgcatg accactcaac
     6721 tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg
     6781 aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg
     6841 tttccggttt tgaaaacccg gaaggttcgc tgaccggggg gattcaggcc acaggcaatt
     6901 atccgggcaa agcgcgtaat gccagtgagc tacgtgccga tctggaacag gctatgcggc
     6961 tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatca gatacgccag
     7021 tctcgcgcga ccagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca
     7081 atcagctcgg tctggatttt aacccctcct gcttttcgca tccgctaagc gccgatggct
     7141 ttacgctctc gcatcccgac gacagcattc gccagttctg gattgatcac tgcaaagcca
     7201 gccgtcgcgt ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct
     7261 ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc
     7321 tggcagcact ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg
     7381 ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt
     7441 acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc
     7501 cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtaccg cagttgctgc
     7561 tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa
     7621 cccaggcaat tgccagtgag attgtgcgtc acgatctgtt tgaccgggtg catatcggcc
     7681 ttgacttctt cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata
     7741 tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag
     7801 cggcgggcga ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgccgcggc
     7861 aggcggtctg ggaaatgtat tgccaacatc acgatacgcc agcaggtagc gaatggctgg
     7921 agagcgtgcg ggcttatgag aaagcgattt tgagccagcg tgggtaaaca ctgccggatg
     7981 cggcacgagc gccttatccg gcctacgggt cgaatgtcca tgcgggctgt caccctcacc
     8041 ccggccctct ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg
     8101 aacaccgctc cggacagtcc cctcgcccct acggggagag ggttagggtg aggggaacaa
     8161 gccgcaccat gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt
     8221 acccagtcct ggtttgtcca gggaatgatc aaagccacca ccgatgccta gctgaaaggc
     8281 tgggacgagc gcaacggcgg caacctgacg ctgcgcctgg atgacgccga tatcgcgctt
     8341 tatcacgaca acttccaccc acaaccgcgc tatattccgc tcagccaacc catgccttta
     8401 ctggcaaata cgccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctc
     8461 gatcctgcag ctaacttagg cgtcgtaaaa gtcgacagcg atggcgcggg ctaccatatt
     8521 ctctgggggt taaccaacga agccgttcca acttctgaac ttccggctca ctttctctcc
     8581 cactgcgagc gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc
     8641 accaacctga tcgccctcac ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa
     8701 ctgtgggaag gcagcaccga atgtctggta gtattcccgg atggcgttgg cattctgccg
     8761 tggatggtgc ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat
     8821 tcgctggtgt tgtggccctt ccacggtgtg ttcggttccg gaccgacgct ggatgaagcc
     8881 tttggtttaa tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg
     8941 ggcggcatga aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc
     9001 gttacaccac tagccagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt
     9061 gtcatgcagg taaaccccga cgcccacgaa gagtatcagc gtcggcataa tcccatctgg
     9121 ccggaactgg aagcagtgtt gaaatctcac ggtgtgcata actacgccat ttacctcgac
     9181 aaagcgcgta atctgctgtt tgccatggta gagattgaat ctgaagaacg gtggaatgcg
     9241 gtagccagta ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct
     9301 aacccagata acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg
     9361 cccaatgtgc cggagatgcc tccggcacaa tattttaaat ttcgatttga aaaattaaac
     9421 atattacatc tcccggtaag ttatatttcc ctgatacatt gtgagtaaat cacaaaaata
     9481 atgaataacc cattaatgat tcatgtggtt tatttaaata accattatgt gcattactcc
     9541 gcaaatctga cctttcactc tgtctcaaat tgtcattgtt taccgacata tcgcctctca
     9601 tcacttaatc gctccaccat tacaattgat taaaatatat tttatggagt ccgattaatg
     9661 gcagctctta ctgcaagctg tattgacctg aatattcagg gcaatggcgc ttattccgtt
     9721 ctgaagcagt tggcgacaat agcgttacaa aacggtttta tcaccgactc acaccagttc
     9781 ctgcaaaccc tgctgctgcg cgaaaaaatg cactctactg gatttggttc cggtgtcgca
     9841 gtgccgcacg gtaaaagcgc ctgcgttaaa caaccgttcg tattattcgc ccgcaaagcg
     9901 caggctattg actggaaagc cagcgatggc gaagacgtca attgctggat ctgcctcggc
     9961 gtgccgcaaa gcggcgaaga ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt
    10021 attcaccagg aatttattca tcaactgcaa cagggcgata ccgaccaggt gctggccttg
    10081 ttaaatcaaa ccctcagctc ataaggaagt ggcgatggag tcatccttac gtattgtcgc
    10141 gatcaccaac tgccccgccg ggatcgctca cacctacatg gtggcggaag ccctggaaca
    10201 gaaagcccgt tctctcggcc ataccataaa agtggaaact caagggtcca gtggcgttga
    10261 aaaccgctta tccagcgaag agattgccgc tgccgattac gtcattcttg ctaccgggcg
    10321 tggcctgagc ggtgatgatc gcgcgcggtt tgccgggaag aaagtttatg agattgccat
    10381 ctccctggcg ttgaaaaata tcgaccagat tttcagcgaa ttaccgacaa actcgcagct
    10441 ttttgccgca gatagcggcg tgaagcttgg caaacaggaa gtgcaaagcg gcagcgtgat
    10501 gagccacctg atggcgggcg tctctgcggc gctgccgttt gtcatcggcg gtggtatcct
    10561 ggtggcgctt gccaacatgc tggtgcagtt cggtttgccg tataccgata tgtcgaaagg
    10621 cgcgccgtcg tttacctggg tagttgaatc catcggttac cttggcttca cctttatgat
    10681 ccccatcatg ggcgcttata ttgcctcgtc gattgccgat aaaccggctt tcgctccggc
    10741 gtttctggtt tgctatctgg cgaacgacaa agcgctgctc ggcacccagt cgggtgcggg
    10801 attcctcggc gcggtggtgc tggggctggc gattggctat ttcgtcttct ggttccgcaa
    10861 agtgcgcctt ggcaaagcat tacaaccgct gctcggttca atgctgatcc cgttcgttac
    10921 tctgctggtt ttcggcgtgc tcacttacta cgttatcggg ccggtgatgt ccgacctcat
    10981 gggcgggctg ctccacttcc tgaataccat tcctccatca atgaagtttg cggcagcgtt
    11041 tctggtcggt gcgatgctgg cgttcgatat gggcggcccc atcaataaaa ccgcctggtt
    11101 cttctgcttc tcactgctgg aaaaacacat ttatgactgg tacgccatcg tcggcgttgt
    11161 cgcgttgatg ccgcccgttg cggcgggtct tgcgacgttc attgcgccca aactgtttac
    11221 ccgacaggaa aaagaggctg ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga
    11281 accggctata ccttacgctc tggcagctcc gttgccgatg attaccgcca atacgctggc
    11341 gggcggcatt accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg cacctggttt
    11401 gggtatcttt gacccgctga tcggcctgat gtcgccggta ggttcgtttt atctggtgct
    11461 ggcgattggc ctcgcgctga acatttcatt cattatcgtc ctgaaaggat tgtggttacg
    11521 ccgcaaagcg aaagccgcgc aacaggagct tgtccatgaa cattgagtta ctgcaacagt
    11581 tgtgcgaagc cagcgccgtt agcggcgatg aacaggaagt tcgcgacatc ctgataaaca
    11641 cgctggaacc ttgcgttaat gaaatcacct ttgatggtct gggcagcttt gttgcccgta
    11701 agggaaataa aggtccaaaa gttgccgttg tcgggcatat ggatgaagtc ggctttatgg
    11761 tcacccacat cgacgagagc ggttttctgc gtttcaccac cattggtggc tggtggaatc
    11821 agtcgatgct caaccatcgg gtaaccatac gcacacacaa gggagtgaaa atccctggtg
    11881 tgattggttt cgtcgcgcct catgcgttaa cggaaaagca aaggcaacag ccgctgtcat
    11941 ttgatgagat gttcattgat attggcgcaa acagtcgcga tgaggtggaa aaacgcggtg
    12001 tggcgatggg cgattttatt agcccggaag ccaattttgc ctgctggggc gaagataaag
    12061 tggtcggcaa ggcgctggat aaccgcatcg gctgcgcaat gatggctgaa ctattgcaga
    12121 cggtgaataa tcccgaaatt acgctgtatg gcgtcggcag cgtggaagaa gaagttgggc
    12181 tacgcggggc acaaacctcg gcggaacaca ttaaaccgga tgtagtgatc gtgctggata
    12241 ccgctgtcgc aggcgatgtt ccgggcattg ataacattaa atacccgctg aaactgggcc
    12301 aggggccggg gctgatgctg tttgacaagc gctacttccc caaccagaaa ctggtagcag
    12361 cgttaaaaaa ctgtgccgca cataacgatt taccgctgca attttccacc atgaaaaccg
    12421 gagcgacgga tggcgggcgc tacaacgtga tgggcggcgg gcgtccggtt gttgcgctgt
    12481 gcctgccaac ccgctatctg cacgctaaca gcggcatgat ttcaaaagcc gattacgatg
    12541 ctctgctcac gttgatacgg ggttttctga cgaccttaac cgcggagaaa gtcaacgcgt
    12601 ttagccagtt ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga ttgtcgatct
    12661 gctggagcaa cagccgcgca cgcctggtga gctggcgcaa cagactggcg tttcaggcag
    12721 gaccatcctg cgtgatattg actatctcaa ctttaccctt aacggcaaag cccgcatttt
    12781 cgccagtggc agtgcgggct atcagctgga aatcttcgag cgccgcagct tttttcagtt
    12841 gctgcaaaag cacgataacg acgatcggct gctggcgctg ttattactga atactttcac
    12901 tccccgtgcg caactcgcct cggcgcttaa tttgccagaa acgtgggtag cagagcgtct
    12961 gccccagtta aaacagcgtt atgaacgcac ttgttgcctg gccagccgcc ctggtttggg
    13021 ccatttcatt gatgagacag aagagaaact cgttatcttg ctggcgaact tgctgcgcaa
    13081 agatccgttt ttaattccgc tggcgggcat aacacgagac aaccttcagc atttatccac
    13141 ggcctgcgac aaccaacac
//
LOCUS       DAJTUP010000102        12876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTUP010000102 DAJTUP010000000
VERSION     DAJTUP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.102"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..404
                     /gene="torD"
                     /locus_tag="OO468_003164"
     CDS             <1..404
                     /gene="torD"
                     /locus_tag="OO468_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR6260177.1"
                     /translation="RDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVE
                     AGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEF
                     AARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(497..802)
                     /gene="cbpM"
                     /locus_tag="OO468_003165"
     CDS             complement(497..802)
                     /gene="cbpM"
                     /locus_tag="OO468_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR6260178.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(802..1722)
                     /gene="cbpA"
                     /locus_tag="OO468_003166"
     CDS             complement(802..1722)
                     /gene="cbpA"
                     /locus_tag="OO468_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR6260179.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1983..3239
                     /locus_tag="OO468_003167"
     CDS             1983..3239
                     /locus_tag="OO468_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR6260180.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     EPFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            3532..4773
                     /gene="agp"
                     /locus_tag="OO468_003168"
     CDS             3532..4773
                     /gene="agp"
                     /locus_tag="OO468_003168"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR6260181.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(4811..5038)
                     /locus_tag="OO468_003169"
     CDS             complement(4811..5038)
                     /locus_tag="OO468_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR6260182.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(5059..5655)
                     /gene="wrbA"
                     /locus_tag="OO468_003170"
     CDS             complement(5059..5655)
                     /gene="wrbA"
                     /locus_tag="OO468_003170"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR6260183.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6028..6135
                     /locus_tag="OO468_003171"
     CDS             6028..6135
                     /locus_tag="OO468_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260184.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            complement(6217..7545)
                     /gene="rutG"
                     /locus_tag="OO468_003172"
     CDS             complement(6217..7545)
                     /gene="rutG"
                     /locus_tag="OO468_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR6260185.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(7566..8060)
                     /gene="rutF"
                     /locus_tag="OO468_003173"
     CDS             complement(7566..8060)
                     /gene="rutF"
                     /locus_tag="OO468_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR6260186.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(8071..8661)
                     /locus_tag="OO468_003174"
     CDS             complement(8071..8661)
                     /locus_tag="OO468_003174"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR6260187.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(8671..9471)
                     /gene="rutD"
                     /locus_tag="OO468_003175"
     CDS             complement(8671..9471)
                     /gene="rutD"
                     /locus_tag="OO468_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR6260188.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(9479..9865)
                     /gene="rutC"
                     /locus_tag="OO468_003176"
     CDS             complement(9479..9865)
                     /gene="rutC"
                     /locus_tag="OO468_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR6260189.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            complement(9877..10569)
                     /gene="rutB"
                     /locus_tag="OO468_003177"
     CDS             complement(9877..10569)
                     /gene="rutB"
                     /locus_tag="OO468_003177"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR6260190.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(10569..11660)
                     /gene="rutA"
                     /locus_tag="OO468_003178"
     CDS             complement(10569..11660)
                     /gene="rutA"
                     /locus_tag="OO468_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR6260191.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMEFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            11948..12586
                     /gene="rutR"
                     /locus_tag="OO468_003179"
     CDS             11948..12586
                     /gene="rutR"
                     /locus_tag="OO468_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR6260192.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
BASE COUNT         3281 a         3243 c         3272 g         3080 t
ORIGIN      
        1 tacgtgacga tgcccgtctg gaactggccg ctgacttttg cggcctgttt ctgatgaccg
       61 acaaacaagc ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa gagattaaac
      121 gcttgttagt tgaggcaggg atggaaacca gtggcaattt caacgaaccg gcagatcatc
      181 tggcgatcta tctcgagttg ttgagttatc tgcatttttc gctgggagag gggaccgttc
      241 ctgcgcgaag aatcgacagt ttgcggcaaa aaacactgac ggcgctgcgg caatggttac
      301 cagagtttgc tgcgcgttgt cgacagtatg acagctttgg tttttacgcg gcactaagcc
      361 agttattgct ggtgttagtg gagtgcgacc accaaaacag ataacgtcgt ttatgcgcct
      421 gaaaagacgc gtttagcgtc gcatcaggca ttatggcgca gttgccggat gcgtcatcac
      481 atccggcaaa cacacatcac ggatgagcta caaaccggga aagccgctgg cgtagcaggc
      541 ggttttcctg cttcaggtgc gcaatatcat ccattaacgt cagcgccacc gcgatccccg
      601 gccagtccag agccagttca tgacgcaggc gtaccgcgcg ttgcaccaca atggcggcat
      661 ggtcgtcaaa tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc cccaaaccga
      721 caatttcatt caactcctct tcagagatgc cggtatgcag gcaaaattcg gtaatagtaa
      781 aagtcaccgt aacattagcc attatgcttt cccccaatct ttacgtggat caaaagacga
      841 ctgggcgtct gccagttgct gccacagcgc ggcagtgttt tcatccggtt tcggcggcat
      901 cacgattttc agtaccgcat acagatcgcc ggtctgtttt ttgctcacca gacctttgcc
      961 tttaacgcgc aatcgttgcc cggcctggct gcctggcggg atagtcagca aaatgctttc
     1021 tttcagtgtt ggaacggtga ctttagtacc cagcgccgct tcccacgggc taaccggcac
     1081 cacaatttcc agatcctggc cgacaatatc gaacagcgga tgtggcgcaa tatgaatcac
     1141 cagccacaaa tcgccatttg gaccgccgtt ttcgcccggc gtcccctggc ctttcaggcg
     1201 gatgcgttga ccattgccga cgcctgccgg gatcttcaca ttcagcgttt tcgggatttc
     1261 ctgttcgatc atgccaaagg cgttataaac cggcaggtta tagctgatgg tacgcttatg
     1321 ctcagtaagc gtttcttcga ggaataccgc cacttcgatt tcaatatcgt ggccacgtgc
     1381 ggcggggcgt tgacggctct ggcgggcatg ctgaccgaaa attgacgaga agatatcgtc
     1441 aaaatcttcg gcattaaaac tctgaccgtc gccatgatgg aactgacggt taaattgcgg
     1501 atcgttgcga tgttgccaca tctgatcata ctcagcgcga cgttgttcat cacttaacac
     1561 ttcccaggct tcagcgacct ctttgaagcg ggcttcggca tccggttctt tgctgacatc
     1621 aggatggtat ttacgggcca gtcgacgata ggcggtcttg attgtcttga gatcgtccgt
     1681 cggtttcacg cccatgatgg cgtaataatc ctttaattcc atagcgttat ctcgcgtaaa
     1741 tcaacacaaa ttgaaggaac ccctgtaagg taactcctat aagtgtaggg taatcctcaa
     1801 aatttcatat gccaacacag aatatgttat tgaaatcatc gcggagagga ggtcgccatc
     1861 aagatgggtt gctgaacata ttttaaacag gtgaaaaagg gtgagcgatt tttgatagtt
     1921 gaaccaggca ctttaagttt aactagggcg tcattattta ttaaatttta tagacgctat
     1981 atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg aaacaggcct
     2041 ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg ctttttgaaa
     2101 acacactggt gtgttttatg cggttgtaca atgaactcag acgtaaagta aatgaagagg
     2161 atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg cagaatgata
     2221 atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt tattcagggg
     2281 tttcaggagg tattacatat actatacaat atgttcaaga tgttgatatt gttagagtgt
     2341 ccttgccggg cagaggttca gagtctatca cagattttaa aggttattat tggtatggct
     2401 ttatggaata tattgaaaac attaatgcgt gtgatgatgt tttttctgag tattgtttag
     2461 ataatgaaaa tatgagtatc cagccagaac agataaatat gccgggaata tctgatttag
     2521 atactggtat tgatttgtct ggcatatctt ttattcagag tgaaattaat aaaacatatg
     2581 gattaaaata cgctccagta gatggcgatg gatattgtct gttaagagcc atactggttt
     2641 taaaagaaca tgagtattca tgggcgctgg gcagccataa gacgcaaaag caagtatacg
     2701 aagagtttat taaaattgtt gataaacaaa cgatcgaggc tcttgttgat acggcattct
     2761 ataatctcag ggaagatgta aagacattat ttggcgttga tctgcaatct gacaacaaaa
     2821 ttcagggtgt gggtagtttt atgtcatgga gctttctgtt ttttaaaaaa caattcattg
     2881 atagttgctt gaatgacaaa aaatgcatac tgcatttacc cgagtttata tttaatgata
     2941 acaagaattt gcttgcttta gataccgaca cgtcggatag gattaaagcg gtgaagaatt
     3001 ttcttgctgt tctttcagat agcatttgct cattatttat tgttaatagt aatgtggcat
     3061 caatctcctt gggaaatgaa tccttttcaa cagatgaaga tcttgagtat ggatatttaa
     3121 caaacactgg caatcattat gatgtttacc tccctcctga acctttttct caggcttata
     3181 agttaaaaaa taaggaaatg aatgcgcaac ttgactattt aaatcgttat gcaacttaat
     3241 ggcaaaggca tatgctaaaa accattgtta ttagtctcac acttttttat tggtaaatat
     3301 tgtctctgta ttggtaacgc cgcagatatt ctgtttagcc acaggtgcaa ttatcagcgg
     3361 cgtacgcgag gcaggggcta atcaggcata gtttgcgtca aaccttgcct gtttttgaag
     3421 atgtatatag aaaaacaggc gttcaacaag ccattttgcg aacctgttcc cggaaaaaag
     3481 tcatatttct gtcacactct ttaatgattg ataacaaaag aggtgccagg aatgaacaaa
     3541 acgctaatcg ccgcaactgt ggcagggata gttttactcg cttcaaatgc tcaggcacaa
     3601 accgtaccgg aaggctatca gctacagcaa gtgctcatga tgagccgcca taacttacgt
     3661 gcgccgctgg cgaacaatgg cagtgtgctg gagcagtcga cgccgaataa atggcccgaa
     3721 tgggacgtcc ccggtgggca actcaccacc aaaggtggcg tgctcgaagt gtatatgggc
     3781 cattacatgc gtgaatggct ggcagagcag gggatagtga aatcggggga atgcccgccg
     3841 ccggacaccg tttatgccta tgccaatagt ctgcaacgta ccgtcgctac cgcccagttc
     3901 tttattactg gtgcattccc tgggtgtgat attcctgtgc atcaccagga aaaaatgggc
     3961 actatggacc caacctttaa cccgatgatc accgatgatt ccgccgcatt cagtgaacag
     4021 gcggtggcgg caatggagaa agagctcagc aaactccagc ttaccgacag ctaccagcta
     4081 ctggaaaaaa tcgttaacta taaagattcc cctgcctgta aagagaaaca acagtgttcg
     4141 ctggtggatg gcaaaaatac ctttagcgcc aagtatcaac aagaaccagg tgtttccggg
     4201 ccgctgaaag tcggcaactc gctggtagat gcgtttactt tgcaatacta cgaaggtttt
     4261 ccgatggatc aggtggcctg gggagaaatc aaatctgacc agcagtggaa ggtattgtcg
     4321 aagctgaaaa acggttacca ggacagtctg tttacctcac cggaagtggc gcgcaatgtt
     4381 gcgaaaccgc tggtcagtta tatcgacaaa gccctggtca ccgatcgcgc cagtgctccg
     4441 aaaatcacgg tgttagttgg gcacgactcc aacatagcct ctctgttaac ggcgctggat
     4501 ttcaaaccgt atcagttgca tgaccagaac gaacgcacgc cgattggcgg caaaatcgtt
     4561 ttccagcgtt ggcatgacag caaagccaat cgcgatttga tgaaaattga atatgtgtac
     4621 cagagtgcgg aacagttacg taatgccgat gcgttaaccc tgcaggcacc tgcgcagcgc
     4681 gtgacgctgg aattaagcgg ttgcccgata gatgccaacg gtttctgccc gatggataag
     4741 tttgatagcg tgttgaatga ggcggtgaaa taacagaaaa ctcccccgcg agaagcgggg
     4801 gagtcgctgg ttaaacgttt ttacgttcga tggtctgttc gccccaaaaa agcgaatctt
     4861 tatcggtctt agcaaaggct ttgactaaca cttcatcact accttcttcc caaatctttt
     4921 ctgccatttt ttcgtcgtac ccggcgactt cgaaaatggc ctcggctatt tccggcgacg
     4981 tattgcgcag agatgcccat tcaccgacgt gatgagcttt cgcttcttga gttggcatgc
     5041 gtatcctcct gttgaagatt agccgttaag tttaactgcc agacctgcga catattcccc
     5101 ttgataacga gcaatagaca gttcttcctg gcttggctgg cgtgagccgt caccgcctgc
     5161 gatggtggtt gcgccgtacg gcgtaccgcc gcgaacctgt gaaacgtcaa ataattcctg
     5221 cgctgcgtag ccaatgggga caatcaccat gccgtgatgc gcaagggtcg tccaggtgga
     5281 tgtgatggtt tgttcctgac cgccgccagt accggtggaa ctaaagacgc tcgccagttt
     5341 tccgtatagt gcgccggaag cccacaggcc gcccgtctgg tcgaggaagg tacgcatttg
     5401 accggacatg ttgccaaagc gggtaggtgt accaaaaata atggcgtcgt aatcggccag
     5461 ttcttgcggg gttgcaaccg gtgcagtttg cgttttaccg cctgcttttt caaataattg
     5521 cggcggcatg gtttccggta cacgcttaac gacaacttct gcgccatcca ctttgcttgc
     5581 accctcagcg actgcgcgtg ccatcgtttc aatatgtccg tacatggaat aataaagcac
     5641 cagaacttta gccatttcta accactcctc gtgttatctc tattccgtag cgattcgcta
     5701 ccacttattt aaagataaga cgtccttttc agagtgcaaa tttcacaacc acttatttga
     5761 tttataacaa ctttcacaag cacgtaattt tgtcgcaaaa tgacacattt ttatctcatc
     5821 gcgttttttt aatcataaga gcggcttatg gataattatt ggagatgata tctattctcg
     5881 ctaagaagct gttgcaggat attaccaaac gcaggtctgt ccgcgtcagt tcactaagct
     5941 tagtcccacg tagcgaaaat atggcagccg ccatacgccg cgttaattct atgcaatatg
     6001 atgtctatac ccagacggag gtcagtaatg gcaaaccatc gaggcggttc cggcaatttt
     6061 gcagaagacc gcgaaagagc atcagaagca ggtaaaaaag gtggcaagag cagtcacggc
     6121 aaaagcgaca actagccggg ctaatcaatg acgaatgcat ttttttctta agctcgtcaa
     6181 aaagtcatca ccgccggtaa cccggcggtt ttctgtttat ggctcctgat gaacaacttc
     6241 tggcggtgga acgtcaacca accttctgct taataacgca ttgagtaaaa tcgcgccaaa
     6301 ggttgctgta ccgatccctc ccaacgtaaa accgccaagc gtgagagcaa aatcacccgc
     6361 gcccagcacg aaggttactg cgaccataat caaattagcg ttctggctta aatcgacacg
     6421 gttttgtacc catatccttg cgcctgcgac ggcaatcagc ccgaacacaa caattgatgc
     6481 accaccaatc accgcggccg gaatggtatg gatcagcgcg ccaaatttcg gcgaaaagcc
     6541 caacagcata gcgatgacgg cagctgcaac aaacaccagc gtcgagtaga cttttgtcac
     6601 ggccatcaca ccgatatttt cagcataggt ggtgacgccg ctaccgccga cagagccgga
     6661 aagcatcgtt gccagaccat caccgacgaa tgctcgcccc atatacgggt ccatattgcg
     6721 tccggtcatc ccggcgaccg ccttgagatg acctaagttt tccgccacca gaatcaccgc
     6781 cacgggcgca atcaacatca tcgcctggcc attaaaacca ggagtggaaa aatgcggcag
     6841 accgaaccag gcagcatagc tgacgagagt aaaatcgacg gcttttccca gccctaaaac
     6901 gttggtcatc acgccataca gcagacaggc gacaattaat cctacgagaa tcaataaccg
     6961 ctggatcatg ccacgggtaa acaccgccac cagcccaata cacagcaccg tcattaccgc
     7021 catccagcta tcaaaggccg aagccgatac acttttcact gcgataggcg ctaagttcag
     7081 gccaatcgcc atcaccaccg cacccgtcac caccggcggc attagtcgtt caatccagcg
     7141 tgtaccaatt ttcatcacca ccaggccaat gacggtataa accagcccac aggcgataat
     7201 cccgcccagc gcgatgctga tattcgggtt aatgccctga ccgttaaagc ccgtcgcggc
     7261 aatcaccacg ccgacaaaag ccgcgctgga gccgagataa ctggggacgc gcccgccggt
     7321 aataaagaaa aacagtaacg tgccgatccc cgacattaaa atggaaagat tgggatccag
     7381 ccccatcaga atcggcatta acaccgtcgc gccaaacatc gccaccgcgt gttgaacgcc
     7441 cattactgcc gtctgagcaa acggcaatcg ttcatccggc gcgaccacgc cgctctctgt
     7501 agaggtcgat tttaactgcc agtgaggaaa accgaacatt gccatcagct gtctccttaa
     7561 ggaggttaac aagcagggcg catcagcgcg tgataactgc gatcgaacca caccagcccg
     7621 tagggtgtgg tgtgacgatg aatcgcttcg atggcgcaaa acagaatgtc gtgggtgccg
     7681 acgctcacca cctggctgat acggcagtca aacgaaacca gagcctcttc cagttgcggg
     7741 catccggtca cacccgtctg ccagcgggcg gcggcaaagc ggtgttccat gggcgttttg
     7801 ccgccaaaaa gatttgaaag cggctcctgc ccggcgctca gtgtatttac acacagcgtt
     7861 cgattttcat tgaataccgg ccagacggac gccccacgat tcaggcacac cagtaatgtg
     7921 ggcggcgtgt cggtcacgct gcagacggcg ctggcggtga acccggcgcg cccggctgga
     7981 ccgtccgtgg tgatgatatt gaccgccgcg cccatacagg acatcgcatc gcgaaaggtt
     8041 tgttgatcaa ctatgttcat agtttgctcc ttacaacagc ccgcaggctt cttcaaagga
     8101 cagacgtggc aggcgcgcaa aaagtttgct gctatcgcca tagccgatat taatcagcag
     8161 attgctcttc agcgtgctgc ccgcaaaaaa ggcgtcgtca acgtgttgac ggtcaaaacc
     8221 cgacatcggg ccggtatcca gtcccagcgc acggcaagcg acgatcagat aagccgcctg
     8281 catggaactg ttgcgaaacg ctgtttcttc ggcaagttgt gggctggagg taaaccaact
     8341 gcgggcatca ccgtggggaa acagtagtgg taaccgttca taaaattcac tgtcccaggc
     8401 gacgatagcg gtgacgggcg cggtcagggt tttttgcaga ttgccgctgg aaagcgccgg
     8461 gcgcagacgt tcttttcctt ctgcagtgcg gataaacacg atccgtgccg gagaacagtt
     8521 agctgatgtc ggcccccatt tcatcagggc ataaagctcc tgtaacgtct catcgctgac
     8581 gggtgtctcc cgccagccgt tgtgagtgcg ggcatcggtg aacagggtgc taagcgcacc
     8641 tgggctaacg gcttcgttca tagcaattcc ttacagggcg gcttcacggt gatgtaacag
     8701 gctggcaagc ccgttgagta acagagcatt aaacgtttcg ggatcggtca cgttgcaggc
     8761 gtgtccgcca tagggcatca ccattttctg gctatcgggc agggcggcat gaagttcact
     8821 ggaacatgct gttggcacca gcagatcatc actggcgcag atgatttgca ccgggcagcg
     8881 gatgcgatcc gcatggtgac taaagtcagc gcgtttgagg gcgttaagtc gacgtagtaa
     8941 attatttttg ccctgaaaat gcgccagtgc cagcgcgtct tctgcctcca ggcgaggtgc
     9001 gcgggccgcc atccagtcgg cgggatagag gaacaacggc tgcgcttcca cccatgcttg
     9061 cgcgccgccg ctatacagta atcgttcgcg aacctgaaaa cagcggcgcg tatgggcgtt
     9121 tattcgtagc cagccgttaa cgctgaccag catagttacc gacgcgggat aatccagcgc
     9181 cagctgcatc cccaccagcg caccgagcgc atggccgacc accgcgtaac gctcaatccc
     9241 tgcggctacc agcgcctgat gcagttccgc tgccatctgg gcgatactgt aatcttctgc
     9301 cagtgtgtcg ggattattgc cggtgccgcg ctggtcgtaa cagactacct gatactcctg
     9361 ctccagcacc gccagttgcg gtaaccagta actgccgcta ccgccaagac ccgaaatcaa
     9421 caccactacg ggtgcatcag cataaggggg agatgagagt gaaagtttca tcgcggcctc
     9481 acttggcgat atgcgcaatt gtggcgattt ccaccagcgc gtcaggtttt accagtccgc
     9541 actgaatgta gaatcgcgcc ggtttatcac ccggaaaaaa ctcggcgtag atttcgttaa
     9601 tcgcggcgta atttttccag tcggtaataa agatgctgtt gaaggtcaca tccgccatcg
     9661 tgccacccgc cgtctcgatc accttgcgga tagtttccag aacgtggcgg gtttgcgcct
     9721 ttgggtcatc ggcaaacagc acgttattat gttgatcaaa agccagcgta ccggagacat
     9781 acaccacgcc atcagccagc gtgccgggaa cgaagggggc cagcggtgcg ctgctgccag
     9841 cgggaataat tacggatttt ggcatcgcta aactccttaa gcgatacgag caaaggacgt
     9901 gggagaaagc gcgtcgcaga acgtttcgac gtcgctgacc cagccaaaaa aggtttcgat
     9961 attgaacaac gcggctttct gtgcaaattc cggccccgcc tggtgtgttg cgtcttccag
    10021 caccacgccg aaatgctcca gaaaaaagcc atcgcgtagc gtcgattcga cgcagacgtt
    10081 ggtagcgata ccggtgaaaa ccagatggcg tattccacgg ctgcgtaaaa tgctgtccag
    10141 cggcgtattg aagaaaccgc tgtagcgcgg cttcggcagc acaatatcgc caggctgcgg
    10201 caccagttca tccaccagtt gataatccca ggagcctttc gccagcaatt tcccctgcag
    10261 ctgcggctgc ttacgcatgg ttttcagggc gttcgattta tgaaaattcg gtgagccggg
    10321 gccgccagcc tcgacatact gttcatccca gccattttga aaccagatga tcaacatccc
    10381 tgccgctcgc gctgcggtca cagcggtttg aatgttggca atgaccgggc gagtggttga
    10441 gacatcaaac ccggcgagat ctaagtagcc gcctggcgtg gcataagcgt tttgcatatc
    10501 caccacgatc tgcgcacttt gctgcggatc gaaggtaatg gcttccggtc gagcggttaa
    10561 ggttgtcatc atgccacctc ctgagtcagc gcagagagat gggcgcggca ctgcatcagt
    10621 ggttgaatgc gctcgccgaa ggtttcgatt cccgacagaa aatcatcgaa ggtcaacagc
    10681 acgccttctg caccaggcac gcttgcgact tcatctaaca tgcgcgcgac actggcgtaa
    10741 gaaccgacta acgtccccat attgatgttt accgccgaag tgggatcggc catctgacga
    10801 acgttggtgt cagtaccgga gcgggtatct ttctgacttt gttcggttag ccagcttaac
    10861 gcctcttcat ccgcgcccgc tttgtagtgt tcccatttgg cgcgagtggc atcgtcggtt
    10921 tcatcggcaa tcaccataaa caacacataa gagccaacgt cgcgtccggt ttgctctgtg
    10981 gcctgtttca tccgcgcagc ggtcggggcg aaagccgtgg gtgtatttac gcctttgccg
    11041 aaacagaagt tgaaatcggc atactgggcg gagaactcca tgccagcgtc gctttgcccg
    11101 gcgcagatca ctttcatggg gatactcggt tgcggactga cgcgacaatc attcatggtg
    11161 aaaaaatcgc ctttaaaatc gcttttcccc gtgccccaca ggtcgcgcag cacctgaaca
    11221 tattcggtga gatagtcgta acgacgggag aaatagtcat cgccaggcca gatacccatc
    11281 tgctcatact cgggcttttg ccagccagtc acgaggttga cgccaaaacg cccgccagag
    11341 atggagtcga tggttgcggc catacgggcg acgattgccg gaggtaacgt taaggtggca
    11401 gcagtggcgt aaatctgaat gcgcgaggtc acggctgcca gacccgccat caaggtgaac
    11461 gactcaaggt tgtgatccca gaactcagtt ttgccgccaa agccacgcag tttgatcatc
    11521 gacagggcga aatcgaaatg gtaatgctcc gctttttgcg caatggcttt attcagttca
    11581 aaggtcggca tgtactgcgg cgcgtgggtc gaaatgagcc agccgttgtt gccaataggt
    11641 acgaatacgc caattttcat catcaacctc tcttcgtctc gtaaagtgaa agtcagacgg
    11701 ggcgctgcat cctgcatatc cttttcagcc gcgtattggc ttgttcgcaa agcggatgcc
    11761 agtttttaaa aagtgaatat tattaatgtg ttagcattgt gttatctaaa atatctggta
    11821 aaaagtggac taaacggtca aaacagttgc acataaaaca tgcatccgtg cgcgatgaga
    11881 gtgcagaagg tcgaggccgg gcgggggttt tgctatcctg ttaccaatct acaagagggg
    11941 agagcgcatg acgcaaggcg cagtgaaaac aacgggtaaa cgttcgcgcg cagtaagcgc
    12001 gaagaaaaaa gcgattctta gcgcagcact ggacactttt tcacaattcg gttttcacgg
    12061 cacaaggctg gagcagatcg cagagttggc gggtgtttca aaaaccaatc tgctgtatta
    12121 cttcccgtca aaagaggcgc tgtatattgc cgtgctgcgg cagattctcg atatctggct
    12181 ggcaccgtta aaagcgtttc gtgaagattt cgccccgctg gcggcgataa aagagtacat
    12241 ccgtctgaag ctggaagtct cacgcgatta tccgcaggct tcgcgcctgt tctgtatgga
    12301 gatgctggca ggcgcgccgt tgttaatgga tgaactgacg ggcgatttga aggcattaat
    12361 tgatgagaaa tcggcgctga ttgccggttg ggtcaaaagc ggcaaactcg cgccgattga
    12421 tccgcagcat ttgattttta tgatttgggc ttccactcaa cattacgccg atttcgcccc
    12481 tcaggtggag gcggtgacag gcgcgacgtt gcgcgatgag gtatttttca atcaaacggt
    12541 tgaaaacgtg cagcggatta ttattgaggg gattcgacca cgttaaagat gacggaggag
    12601 gttgtaacat cctccggcta cctgtttaac cgatcgtcat taaactggcg ttaccgccag
    12661 cggcagcggt attcacactc agcgaacgtt catcgatagg aattaaaacc ccaaaaagat
    12721 taaaaaaaca ccacaaaacg gatgtttctt caacaccact tttgctccat atgaacggaa
    12781 ccgacgatta aactggatgg ctctgattga ttcagggtat gaatggcggt tttttgctcc
    12841 gtttccctca aaatggacgc aacttcccct ctgcgg
//
LOCUS       DAJTUP010000103        12672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTUP010000103 DAJTUP010000000
VERSION     DAJTUP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.103"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1344)
                     /gene="uxaB"
                     /locus_tag="OO468_003180"
     CDS             complement(<1..1344)
                     /gene="uxaB"
                     /locus_tag="OO468_003180"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR6260193.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDH"
     gene            complement(1571..2989)
                     /locus_tag="OO468_003181"
     CDS             complement(1571..2989)
                     /locus_tag="OO468_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR6260194.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3143..3487)
                     /locus_tag="OO468_003182"
     CDS             complement(3143..3487)
                     /locus_tag="OO468_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR6260195.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3487..4413)
                     /gene="glsB"
                     /locus_tag="OO468_003183"
     CDS             complement(3487..4413)
                     /gene="glsB"
                     /locus_tag="OO468_003183"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR6260196.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4477..5412)
                     /locus_tag="OO468_003184"
     CDS             complement(4477..5412)
                     /locus_tag="OO468_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR6260197.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5492..6607
                     /gene="yneK"
                     /locus_tag="OO468_003185"
     CDS             5492..6607
                     /gene="yneK"
                     /locus_tag="OO468_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR6260198.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6757..7947
                     /gene="ydeA"
                     /locus_tag="OO468_003186"
     CDS             6757..7947
                     /gene="ydeA"
                     /locus_tag="OO468_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR6260199.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(7972..8637)
                     /gene="marC"
                     /locus_tag="OO468_003187"
     CDS             complement(7972..8637)
                     /gene="marC"
                     /locus_tag="OO468_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR6260200.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            8849..9283
                     /gene="marR"
                     /locus_tag="OO468_003188"
     CDS             8849..9283
                     /gene="marR"
                     /locus_tag="OO468_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR6260201.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9303..9686
                     /gene="marA"
                     /locus_tag="OO468_003189"
     CDS             9303..9686
                     /gene="marA"
                     /locus_tag="OO468_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR6260202.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9718..9936
                     /gene="marB"
                     /locus_tag="OO468_003190"
     CDS             9718..9936
                     /gene="marB"
                     /locus_tag="OO468_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR6260203.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(9967..10866)
                     /gene="eamA"
                     /locus_tag="OO468_003191"
     CDS             complement(9967..10866)
                     /gene="eamA"
                     /locus_tag="OO468_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR6260204.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11130..12248
                     /gene="ydeE"
                     /locus_tag="OO468_003192"
     CDS             11130..12248
                     /gene="ydeE"
                     /locus_tag="OO468_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR6260205.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12375..12470
                     /gene="mgtS"
                     /locus_tag="OO468_003193"
     CDS             12375..12470
                     /gene="mgtS"
                     /locus_tag="OO468_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR6260206.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3329 a         3241 c         2898 g         3204 t
ORIGIN      
        1 gtggtctttt tctgccaaca caatcgctac cagttcctgg gtaccgataa cacggtcacg
       61 atgctggctc cagagttgct gataacgttc cagccagtgt gcatcgtcct gtaccggata
      121 tgtttctccg ttacgctcac cacgatagaa cgcaatcaat gccgctaatg cgaaagtaag
      181 gcgcgccgga agtgtgccat ttgccttctg ccctgccagc agctgcggca ggatgcgggt
      241 gcggaacttg gtcataccgt tgagcgcgat agacagcagc tgatgcttaa tgtacgggtt
      301 acggaaacgc ccggtgactg cactggcgaa agattccagt tcatcacgag gcaaatccag
      361 taccggaata atttcttcgt agatggcttt ttcaacgaat gcgcaaattt cagcatcgtt
      421 catcgcttca cctacggtat ccagccctgc ctggaacgcc accggcacca gcgcggtgtg
      481 cgcaccgttg aggattgcca ctttgcgttc tttatacggt ttaatatcgt caacaatcag
      541 cacgttgagc ggatatttgt ccaggcgcag ctcagtcgct aaggatttcg gcccctgaat
      601 cacaaacagg taaaagtgtt cagcggtgtc gaggaaacca tcgtgataac ccagttcttc
      661 ttccagttta gccacttcat cgcgcggata accggtaacg atacggtcca ccagcgtaga
      721 acagaagctg ttagcctgat ccagccattg aataaatgct tctggcagtg cccactcttg
      781 cgcatagcgc agcaccagtt cacgcaacgc gtcgccattg tagtcaatca actcacacgg
      841 aatgatgatc caacctttat ccagcgcacc gttgaaatgg ctaaagcgtt cgaacagcag
      901 acgggtcagt tttgccggat agcttaccgc tggcgcgtca tcgaatttat cgcccgcatg
      961 gtagctgata cccgcttcgg tggtgttaga gaaaacaaag cgcatttccg ggttgtgcgc
     1021 cagtttcagg aattcatcgt attcactgta gacgctgatt tcacgattaa ccgagcgaat
     1081 cagacgcgcg tcgctgaccg cttccccctt ctcattcagg ccacggataa tggtggtgta
     1141 cagaccatcc tgcgtgctca gtgacggcgg gaatgaagtt tcaatcggac gaacaacgac
     1201 cacgccagaa ttcagatcgg tgtgctcatt caggagatcg atttgccagt caacaaaggc
     1261 gcgcaggaag ttaccttcac caaactgaat gatacgttct ggatactgtg caccgggaaa
     1321 atcgcgacga tttagtgttt tcacaatggg ttcccttctg attagtcata caacccgttt
     1381 gaattggtgc gacaggttag caaactttaa tacgccgaac ccctgttttg atcaactcct
     1441 gatgattaat gagcagtttt atgagaaaag tgtggcgcgg atcatggttt aatcgaggaa
     1501 atacgccttt tcctggatca taaagtggta gaacacattg cattcaaatc gcgcggaatg
     1561 aataaagatg tcagacaact tcctcaccgt aacgcatagt gctggtacgg ttgcgcccat
     1621 ctttcttcga gcgatacaga cacgtatcgg cttcgaccat caatttatta aaatcatcgg
     1681 tcagcgttcg gtacgatgcg cgaccactac cgacgccaat acttaccgtg agataaagcg
     1741 ttttttgttg ccaggtgaat ggttgcagtt caacgccttt acggattttt tccgccatta
     1801 gcagaccatc gacaggattc accgacggca ctgcaacagc aaattcttcg ccgcccatgc
     1861 gcgccaccag ccccttatca ccgacaatct tctgaatatg ccgggcaaac acgcttaaca
     1921 ctttatcgcc acattcatgt ccatagttat cgttaatgct tttgaaatag tcgatatcaa
     1981 gcagcatgac cgtcagatgt tgtgtctgtt tcagcgatgg acttttcagc gcctcataaa
     2041 gaccggaccg tgagtaaacc tgagtcagaa aatcaaagtc ggctcgcagc gcaacttgct
     2101 tcattagcaa attgatcgct gccacgctaa aagaaaccat aattgggcat atcgccatcg
     2161 tggcaatacc gagacgtgcg gagaacattt ccggaatgga gaacggcgaa ccgaccgaga
     2221 tatcaatcac cgaatttgcc accagtacga tttccaccgc accggtgaca aaggtcaaca
     2281 gacatgttac ctgcggcgta tagcgcactg cacaccagat taatgcaggc agcgggaacg
     2341 ccagactccc cgctccgcca atgactaccg aggcaataac cgacacaata aacgcgatgg
     2401 cgggcatcat ctgttctgct ttaaagcgcg gcagcactcc aggaatagcc aacgtcagca
     2461 tacaaggcac gatcaacacg cccgttgaga attgctcact gaaccagtcc gcaagcaaag
     2521 gccagaaatc cagactatca atactgaccg aaccaatcgc cccgacaata gcgcataata
     2581 atgcggcaat cagacagtaa ttaaatagcc gtaaagcact tactggctca tacttatttt
     2641 tccccagacg cttatcccgc acgaccagta aggcgacggt aacaataaac atcatattgg
     2701 agaaattgat aaccagtgaa accagtcccc attcggtggt tatggcatca taaaccagca
     2761 tcgccacata actgatcgca tagtaatgca gtcgattaag ccagacatag cgggcaaaca
     2821 cccccgccat tacgccattc agcggccaga aaagagaaag ttcgtgaacc agccgtaact
     2881 ctgcaccaat aaaatagaac agcgtggtta gcgcaaaaat agcgatgcta ttacgtagca
     2941 gatgaccttc ctggaacaaa cggaaagttg aaatggggtg tacatgcatt aattatctgt
     3001 ccatacaatt gcgggtggca attatctcaa cgtgataagt aaattctcat aaaggatagc
     3061 atgttcgcta acagcggcgg caaataaagg taccctggga atgctatttt atcgttttcc
     3121 gcaattctca tggctgattc atctatacaa ccagccagtg atagattacc gccacgatat
     3181 aacgttgttg ttcttcgctt aacgaaacac cctgcggaat gttatgccac ttagccagac
     3241 aaccgcgaca gcaggtcgcg gtggcgtgct gagcgataaa caccggatgc ccgcgcatgg
     3301 gggtttgctt accgtcatta gccggtaatg ccggagcaag acgtttagcg acaaaatccg
     3361 ccgcatgttg ttcgatgact ggcgcgcctt tctccaggca atactgacgc tctttcatgc
     3421 ccagacgaaa gcgagagcga aattttgaac gggataaacg cgcgaagagt ggatcgagag
     3481 actgcattaa taaaccgaac gccctaactg tttcgtcaat tgttcaagga cggcgatacc
     3541 cgcaagcgag ttacctgcat catccagttc cggactccag actgcgatgg ccatttcatg
     3601 cggaacaatc gccacaatac cgccaccaac gccagatttc gccggtagtc ccacccgcca
     3661 ggcaaactcc cccgcgttct ggtacatacc actggtcgcc atcagcgcgt taatttgccg
     3721 cgcctgcatt ggcgtcacca caggttcatc aatatgaata gctttcccct gattagccag
     3781 aaagacaaac gtccgggcca gctctacaca gctcattttc agagcgcagt aatgaaagta
     3841 gttttgcaga acggttgtca cgtcatgatg gaaattgcca aacgacttca tcagccaggc
     3901 gatagccgca tttcgcgcgg aatgttcaaa ttcggaacgc gctaccaccg tatcgtatga
     3961 aatatcagac acaccgctta agccgcgcac gacttccagc atacgttgcc gtggtgcgct
     4021 tagtcgccct tgcaacatat cgcagaccac cagcgcaccg gtattaatga acggattacg
     4081 cggaataccc tgttccattt ccagttgcac taaagaattg aacggtgatc cagacggatc
     4141 tttgccgacg cgttgccaga tttcctcttc ggagtaatga cgcatggcga caacgagact
     4201 cagcactttg gaaatagact gaatggaaaa acgttcttgc gcgtctccgg cctgaaaaag
     4261 ctgtccgtca acggtacaga tagcaatccc caatcgggaa ccgtctactg tagccagcgc
     4321 cggaatataa tccgcgactt taccctgacc aatgagcggc cgcacttgcc gcaagatgtt
     4381 ttctaaaatt gcattatcca tggcgactgc cactttctac tcctggaccg caggtctgaa
     4441 aagacctgcg agtatatcag agctgaatat gtcgcgtcag atccggtctt tccacactgt
     4501 ctggatatta cagaattcgt gtaagccgaa atgggaaagc tcacgaccaa agccactctt
     4561 tttcacgcca ccaaaggcca ctcgcgcgtc gctggcacaa taaccattga taaacacccc
     4621 accgcattcc agacgtgccg ccatctgtct ggcctgtgtt tcgtcggtgg taaaaatggt
     4681 cgctgaaagg ccgaactcac tatcattagc cagttccagc gcatgttctg catctttcgc
     4741 aatggtgatt gcagcaacgg ggccaaacat ttcttcccga aacgcggtca tttctggggt
     4801 aacattcgcc agaaccgttg gcggatagta gttacctgct ccagccatct tttccccgcc
     4861 cagtaacaaa cgcgcaccct gcgccagggt tttctccacc tgatgatgca gctcatcacg
     4921 taaatcaaaa cgagccattg gtccgagagc gttctcttca tcacggggat cgcccatttt
     4981 caaggctgcc gcagctgaca caaaacgttc ggtaaatgcc gaagcaattc cctcttcgat
     5041 aataaagcgt tttgccgctg cacaaacctg tccggtattc tgataacgtc cggctaccgc
     5101 cgctttaacc gccagttcca gatcggcatc gttaagcaca ataaatggat ccgaaccgcc
     5161 cagttccagt acgcattttt tcagtgccgc tccagcacga acacttccgg tcaccgtgac
     5221 agcagcaatg cgcgagtctt taatcatctg actgacaccg tcgttgtcgg cattcagcca
     5281 gccatatacg ccttgcggga ttcccgcatc tttaaacact tgggcaatga gctgagcaca
     5341 gcccatcaca ttcggcgcat gtttaagtaa gtagccgtta cctgcaagaa tgatgggaac
     5401 agcgccacgc atcacctgcc tttgatagtt cttgcagctc aaatgcccta tatagtgatg
     5461 ttccgatgac ttatgactat atggggcaaa tatggttacg ccagtaagca tctgcaatta
     5521 catatctctt cctgatgatt ttcccgctcg taatattgca cctcaagtaa aagaagtttt
     5581 aaaggatttt attgatgcac tcagtaccat aatatgtgat gaagagtggc gcacgtcttt
     5641 aaacaccaac tcagccacaa aaaagatatt taacaacctt gataacttat catatattca
     5701 gaggacttct tttcggggta acgacacact atacaatgaa aaggttcagt ttaaactcac
     5761 ttatcctgtc aagaatggaa gacacaaaga aaatattgaa tttcaggtag taataaattt
     5821 aagccctatt tatttagata attttcgcca tgatggagaa attaatattt tttgcgctcc
     5881 caacccaaag cctgtcacta tggggcgcgt atttcagacc ggcgtcgagc gtgtgctctt
     5941 tctgtttatg aatgatttta ttgaacaatt tccaatgatc aaccttggcg cccccatcaa
     6001 aagagcgcat acaccacata ttgaacccct gcctccggat caccataccg ctgcagatta
     6061 cttacgccag tttgatttgc ttgtcctgaa ttttatctct cgcggtaatt ttgtcatact
     6121 cccccgatta tggaataact ctgaggttca cagatggttc gtcaataaag atcctaactt
     6181 gatcaccgcc attctcgaca taacggacag cgaattaaaa gaggatttat tgcaaagcct
     6241 gatggattca ttaggttcta acaaacatgt acaacccgaa gtctgcatct gcttcttatc
     6301 ccttttagca gaacaagaat ctcctcattt tcaggacttg tttttatttt tcgccaatat
     6361 gttattgcac tatcaccaat ttatgaatcc caatgaaagt gatctgaatg acgtgttgat
     6421 gccagcatca ttaagtgatg ataaaattat caaacatatg gcgcgcagga ccctcaaact
     6481 gtttgtaaaa aatgaaacgc cgccaaaagt tactcacgaa gacctggtga aaaacaggcc
     6541 tcgctcccct gtcagaccac ctatacccgc aaccgccaaa acgccagacc tccctgaacg
     6601 tcattaaacc gtgatgttac cgactctctg actcggaaca gaatcagcgt cagagaaacg
     6661 gaaaacgcga tccagatcac aaatgcattg tattcacatc attaaccgtt ttaagatcat
     6721 ttcatcactt tttcgcaact cacccgataa tctgttatga caacaaacac tgtttcccgc
     6781 aaagtggcgt ggctacgggt cgttacgctg gcagtcgccg ccttcatctt caacaccacc
     6841 gaatttgtcc ctgttggcct gctctctgac attgcgcaaa gttttcacat gcaaaccgct
     6901 caggtcggca tcatgttgac catttacgca tgggtagtag cgctaatgtc attgcctttt
     6961 atgttaatga ccagtcaggt tgaacggcgc aaattactga tctgcctgtt tgtggtgttt
     7021 attgccagcc acgtactgtc gtttttatcg tggagcttta ccgttctggt gatcagtcgc
     7081 attggtgtgg cttttgcaca tgcgattttc tggtcgatta cggcgtctct ggcgatccgt
     7141 atggctccgg ccgggaagcg ggcacaggcc ttgagtttaa ttgccaccgg tacagcactg
     7201 gcgatggtct taggtttacc gctcgggcgc attgtggggc agtatttcgg ctggcgaatg
     7261 accttcttcg cgattggtat tggggcgctt gttacccttt tgtgcctgat taagttactt
     7321 cccttactgc ctagtgagca ttccggttca ttgaaaagcc tcccgctatt gttccgccgc
     7381 ccggcattga tgagcattta tttgttaact gtggtggttg tcaccgccca ttacacggca
     7441 tacagctata tcgagccttt tgtgcaaaac attgcgggat tcagcgccaa ctttgccacg
     7501 gcattactgt tattactcgg tggtgcgggc attattggca gcgtgatttt cggtaaactg
     7561 ggtaatcaat atgcgtctgc gttggtaagt acggcgattg cgctgttgct ggtgtgtctg
     7621 gcactgctgc tacctgcggc gaacagtgaa atacacctcg gggtgctgag tattttctgg
     7681 gggatcgcga tgatgatcat cgggcttggt atgcaggtta aagtgctggc gctggcacca
     7741 gatgctaccg acgtcgcgat ggcgctattc tccggcatat ttaatattgg aatcggggcg
     7801 ggtgcgttgg taggtaatca ggtgagtttg cactggtcaa tgtcgatgat tggttatgtg
     7861 ggcgcggtgc ctgcttttgc tgcgttaata tggtcaatca ttatatttcg ccgctggcca
     7921 gtgacactcg aagaacagac gcaatagttg aaaggcccat tcgggccttt tttaatggta
     7981 cgttttaatg atttccagga tgccgttaat aataaactgc acacccatac ataccagcag
     8041 gaaccccatc aggcgggaga tcgcttcaat gccacccttg cccaccagcc gcataattgc
     8101 gccggagctg cgtaggcttc cccacaaaat aaccgccacc aggaaaaaga tcagcggcgg
     8161 cgcaaccatc agtacccaat cagcgaaggt tgaactctga cgcactgtgg acgccgagct
     8221 aataatcatc gctatggttc ccggaccggc ggtacttggc attgccagtg gtacaaaggc
     8281 aatattggca ctgggttcat cttccagctc ttccgacttg cttttcgcct ccggtgaatc
     8341 aatcgctttc tgttgcggaa aaagcatccg aaaaccgata aacgcgacga ttaagccgcc
     8401 tgcaattcgc agaccgggaa tcgaaatgcc aaatgtatcc atcaccagtt gcccagcgta
     8461 atacgccacc atcatgatgg caaatacgta caccgaggcc atcaacgact gacgattacg
     8521 ttcggcactg ttcatgttgc ctgccaggcc aagaaatagc gcgacagttg ttaatgggtt
     8581 agctaacggc agcaacacca ccagccccag gccaattgct ttaaacaaat ctaacattgg
     8641 tggttgttat cctgtgtatc tgggttatca gcgaaaagta taaggggtaa acaaggataa
     8701 agtgtcactc tttagctaac cttgcatcgc attgaccaaa acttgaaccg atttagcaaa
     8761 acgtggcatc ggtcaattca ttcatttgac ttatacttgc ctgggcaata ttacccccta
     8821 caactaatta cttgccaagg caactaatgt gaaaagtacc agcgatctgt tcaatgaaat
     8881 tattccattg ggtcgcttaa tccatatggt taatcagaag aaagatcgtc tgcttaacga
     8941 gtatctgtct ccgctggata ttaccgcggc acagtttaag gtgctctgct ctatccgctg
     9001 cgcggcgtgt attactccgg ttgaactgaa aaaagtgttg tcggtcgacc tgggagcact
     9061 gacccgtatg ctggatcgcc tggtctgtaa aggctgggtt gaaaggttgc cgaacccgaa
     9121 tgataagcgc ggcgtactgg taaaacttac caccagcggc gcggcaatat gcgaacaatg
     9181 ccatcaatta gttggccagg acctgcatca agaattaaca aaaaacctga cggcggacga
     9241 agttgcaaca cttgagcatt tgcttaagaa agtcctgccg taaacaaaaa agaggtatga
     9301 cgatgtccag acgcaatact gacgctatta ccattcatag cattttggac tggatcgagg
     9361 acaacctgga atcgccactg tcactggaga aagtgtcaga gcgttcgggt tactccaaat
     9421 ggcacctgca acggatgttt aaaaaagaaa ccggtcattc attaggccaa tacatccgca
     9481 gccgtaagat gacggaaatc gcgcaaaagc tgaaggaaag taacgagccg atactctatc
     9541 tggcagaacg atatggcttc gagtcgcaac aaactctgac ccgaaccttc aaaaattact
     9601 ttgatgttcc gccgcataaa taccggatga ccaatatgca gggcgaatcg cgctttttac
     9661 atccattaaa tcattacaac agctagttga taacgtgaca acgtcactga ggcaatcatg
     9721 aaaccacttt catccgcaat agcagctgcg cttattctct tttccgcgca gagcgttgcg
     9781 gaacaaacca cgcagccagt tgttacttct tgtgccaatg tcgtggttgt tcccccatcg
     9841 caggaacaac caccgtttga tttaaatcac atgggtactg gcagtgataa gtcggatgcg
     9901 ctcggcgtgc cctattataa tcaacacgct atgtagtttg ttctggcccc gacatctcgg
     9961 ggcttattaa cttcccacct ttaccgcttt acgccaccgc aagccaaata cattgatata
    10021 cagcccggtc ataatgagca ccgcacctaa aaattgcaga cccgttaagc gttcatccaa
    10081 caatagtgcc gcacttgcca gtccgactac gggcaccagt aacgataacg gtgcaacccg
    10141 ccaggtttca tagcgtccca gtaacgtccc ccagatccca taaccaacaa ttgtcgccac
    10201 aaacgccaga tacatcagcg acaagatggt ggtcatatca atagtaacca gactgtgaat
    10261 catggatgcg gaaccatcga gaatcagcga gacaacaaag aagggaatga ttgggattaa
    10321 agcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca tgatcttttt
    10381 attgaagatg ttgccacacg cccaactaaa tgctgcagcc agggtcaaca taaagccgag
    10441 catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc caaaaatcgc
    10501 taaggcgatc cccgccaatt gtttgccatg cagtcgctcc ccgaaagtaa acgcgccaag
    10561 cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag caggcatacc
    10621 aaagttaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa ctgatggtta atccataccc
    10681 caagagcaaa ttcagtggta ctttcggtcg tgcaacaaaa aagatggccg gaaaagcgac
    10741 cagcataaag cgcaaaccgg ccagcatcag cggtggcatg ttatgaagcc ccactttgat
    10801 gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc catcttttcg
    10861 cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc tgaatttact
    10921 tttctttaac agttgattcg ttagtcgccg gttacgacgg cattaatgcg caaataagtc
    10981 gctatacttc ggatttttgc catgcgtccg atggttaata acaacgacga ggcaagaagg
    11041 gcgctggtag agcgtcgtag ggataagttc atttgtctgt ccggcagtgc gtttcgttat
    11101 gttttatttt agagatgtaa agaaatagca tggctacgct gccatttatg accatttact
    11161 tgagtcgcca gtacagcctg agtgtcgatc taatcggtta tgcgatgaca attgcgctca
    11221 ctattggcgt cgtttttagc ctcggttttg gtatcctggc ggataagttc gacaagaaac
    11281 gctatatgtt actggcaatt accgccttcg ccagcggttt tattgccatt cctttagtga
    11341 ataacgtgac gctggttgtg ctcttttttg ccctcattaa ctgcgcctat tctgtttttg
    11401 ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc caccagcaaa acgaaaatct
    11461 tctcaatcaa ctacaccatg ctaaacattg gctggaccat cggtccgccg ctcggcacgc
    11521 tgttggtgat gcagagcatc aatctgccct tttggctggc agctatctgt tccgcgtttc
    11581 ccatgctttt cattcaaata tgggtaaagc gcagcgagaa aatcatcgcc acggaaacag
    11641 gcagtgtctg gtcgccgaaa gttttattac aagataaagc actgttgtgg tttacctgct
    11701 ctggttttct ggcttctttt gtaagcggcg catttgcttc atgcatttca caatatgtga
    11761 tggtgattgc tgatggggat tttgccgaaa aggtggtcgc ggttgttctt ccggtgaatg
    11821 ctgccatggt ggttacgttg caatattccg tgggccgccg acttaaccca gctaacatcc
    11881 gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg actggtcggt tttatttttt
    11941 ccggcaacaa cctgctattg tggggtatgt cagctgcggt atttactgtc ggtgaaatca
    12001 tttatgcgcc gggcgagtat atgttgattg accatattgc gccgccagga atgaaagcca
    12061 gctatttttc cgcccagtct ttaggctggc ttggtgccgc gattaaccca ttagtgagtg
    12121 gcgtagtgct aaccagcctg ccgccttcct cgctgtttgt catcttagcg ttggtgatca
    12181 ttgctgcgtg ggtgctgatg ttaaaaggga ttcgtgcaag accgtggggg cagcccgcgc
    12241 tttgttgatt taagtcgaac acaataaaaa tttaattcag ccttcgttta ggttatctct
    12301 gctaatatct ttctcattga gatgaaaatt aaggtaagcg aggaaacaca ccacaccata
    12361 aacggaggca aataatgctg ggtaatatga atgtttttat ggccgtactg ggaataattt
    12421 tattttctgg ttttctggcc gcgtatttca gccacaaatg ggatgactaa tgaacggaga
    12481 taatccctca cctaaccggc cccttgttac agttgtgtac aaggggcctg atttttatga
    12541 cggcgaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga tttgtgtttt
    12601 gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac agtagcatca
    12661 gttttctcaa tg
//
LOCUS       DAJTUP010000104        12420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTUP010000104 DAJTUP010000000
VERSION     DAJTUP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.104"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..893)
                     /locus_tag="OO468_003194"
     CDS             complement(<1..893)
                     /locus_tag="OO468_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6260207.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPG"
     gene            1134..1934
                     /locus_tag="OO468_003195"
     CDS             1134..1934
                     /locus_tag="OO468_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR6260208.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="OO468_003196"
     CDS             2012..2782
                     /locus_tag="OO468_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6260209.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO468_003197"
     CDS             complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO468_003197"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR6260210.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO468_003198"
     CDS             complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO468_003198"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR6260211.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5037..5759
                     /locus_tag="OO468_003199"
     CDS             5037..5759
                     /locus_tag="OO468_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6260212.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO468_003200"
     CDS             complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO468_003200"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR6260213.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO468_003201"
     CDS             6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO468_003201"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR6260214.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7152..7228
                     /locus_tag="OO468_003202"
     tRNA            7152..7228
                     /locus_tag="OO468_003202"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7186..7188,aa:Asp,seq:gtc)
     gene            complement(7359..8405)
                     /locus_tag="OO468_003203"
     CDS             complement(7359..8405)
                     /locus_tag="OO468_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR6260215.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO468_003204"
                     /pseudo
     CDS             complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO468_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO468_003205"
     CDS             complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO468_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR6260216.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO468_003206"
     CDS             complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO468_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR6260217.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11365..12195)
                     /locus_tag="OO468_003207"
                     /pseudo
     CDS             complement(11365..12195)
                     /locus_tag="OO468_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12182..>12420)
                     /locus_tag="OO468_003208"
     CDS             complement(12182..>12420)
                     /locus_tag="OO468_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TagK domain-containing protein"
                     /protein_id="HCR6260218.1"
                     /translation="SIDAILNVLDATGEDETIWPVEKTPPDILHLLSPEYAPETAHNT
                     VLPDLTRKEHRIIGIDSHYRINPAQHGEIYHDKQ"
BASE COUNT         2843 a         3242 c         3315 g         3020 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg
      841 ccgctttcga cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct
      901 cctgttgcaa aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt
      961 gatatgtcat ccgatttaat gttctcaata atgagcaaat ttctgacagg tgtaagcact
     1021 tgcttacata acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt
     1081 agaaccctcc cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa
     1141 aaaacaccta tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg
     1201 ggtcttttgc gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc
     1261 gtattcggat cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat
     1321 taaagaacta cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag
     1381 agttagtaca gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc
     1441 tgccacaaca tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc
     1501 cgttacgtga acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc
     1561 cattacctga cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg
     1621 gcgtggatgt ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg
     1681 gcccggtcat tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat
     1741 atcgctttgc gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc
     1801 gggcgtttgg ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt
     1861 ctgtattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg caccaatcgt caaactttgg cctggcttca gcttagatcc
     3061 gcgcagtttg ttccactgct gcaaatcttt ggtgcttacg ccgagacgtg aagcgatgct
     3121 tgaaagcgtg tcgccagagc gtacggtgta aacacggctg ttaagcggcg tattgttggc
     3181 aaccagcgtc gactgtacag cagcaatttc gcctgaagcg agagattcac gcagttgatc
     3241 tgcatgcttc tttggcacca tcacgtactg cggcccactt gcgcccagcg tggagccttt
     3301 cacgccagcg ttgaatgtct tcagcttgct gacggaaatc cccgccatat ctgcaacctt
     3361 cgccatttca accgggctac tcaggtgcac acgcgccaga gcacggcttt catcggtcgt
     3421 tggcagacgt acgccataac gcttgctgtt tttgagaata tcactcaatg ccagcatttt
     3481 aggcacgtac tgcttcgttt cctgcggcaa cggtaacgac cagaagtccg tggatttccc
     3541 acgcgctttg ttcgttttaa ttgccttcat gacccgacct tcgccgctgt tataagccgc
     3601 tacggtcaga agccagtcgt cgtcaaacat tttgttcaga cgctgcatca tattcagcgc
     3661 ggcagttgtt gaagcaacaa catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc
     3721 ataattgcgc cccgtgctcg gaatgatctg ccagatgcct gcggcattgg cgccagacgt
     3781 tgcgtgagga tcaaaagcgc tctccactat gggtagtagt accagttcca taggcatgtt
     3841 acgtttttta acttgccctg ctatccagta catatacggc tctgcccgta aagttacatc
     3901 gtggagatag ctcttattgc gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc
     3961 cggaattccc atctttagct cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat
     4021 agacgtccca tcgtccatcc atcgtgcctg acttgtaaac tttgctgctt ccccttgacc
     4081 agctgcagaa aggctctgtg cgtgctgttg aacgttgccg gtactctggc aacccacgag
     4141 caggacagag gcgagtaata tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa
     4201 gacgaccgat cataacggcg aacggagccg atgacaagaa agttttatca gaacctatct
     4261 ttctttgacc ttaaccatgc aaaacgctct tcaggttgtt gcaataatgt ttcttcatta
     4321 attccattaa ttaaatcaat atcttccgtt cttaaaaaaa cattaatttg ccgctcattt
     4381 ttcagaatta cgggtagtgt tatttgattt tttgcccgta actccttaac tttacgataa
     4441 taatcattta tggacaaatc gtgcggaaaa atactcaaag caaacttcat atttgataac
     4501 gtatattcat gagcacaaca taccaatgta tcgtcaggta acgcacttag ctttttaagt
     4561 gattgataca tttgtaaagc tgtcccttca aacaaccgac cacacccacc agaaaacagc
     4621 gtgtcgccgc aaaatagata aggtttactg aagtaacaga tatgtcctaa agtgtgaccc
     4681 ggtgtggtaa ttacactaaa ttcatgccct aaaacgaagg cagtttcgcc atctttgact
     4741 acctgtgttg ttcccttatc ttgtgtctct tgtggaccat acactacaat ttgcggaaac
     4801 ttttccacca gttcttttac gccgccaacg tgatcgtggt gatggtgggt tagaaatatg
     4861 gcctccggtt gccagttatt ggcggtaatg gcgtttaata ctggctctgc gtctccggga
     4921 tcgacaatca ggcagcgacc tgcctcatca ttcaaaaccc agatgtaatt gtcatcaaag
     4981 gcgggaatac tgttaagatt catagattac ctctcagtgt aaaacggaag gttgtgatga
     5041 aaccggcaag agtccctcaa actgtcgtgg ctcctgattg ctggggcgat ttgccctggg
     5101 gagagcttta tcgcaaggcg ctggagcgcc agctcaaccc gtggttcact aaaatgtatg
     5161 gttttcatct gcttaagatt ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg
     5221 tttctcatca agtgaatgtt tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac
     5281 ttcatcttcc ttttgccgat aaatccgttg atatttgtct actggcacat accttgccgt
     5341 ggtgcaccga tccgcatcgt ttattacgtg aagccgatcg ggtactgatt gatgatggct
     5401 ggctggtgat tagtggcttc aaccccatca gtttaatggg attacgcaaa cttgtgccgg
     5461 tattgcgcaa aacctcgccc tataatagcc ggatgtttac tctgatgcgg caactggact
     5521 ggctctcttt gttgaatttt gaagtgctgc acgccagccg tttccacgtt ctcccgtgga
     5581 acaaacacgg aggaaaacta ttgaatgcgc atattcctgc gcttggttgc ttacaactta
     5641 ttgttgcccg gaaacggact attcctttaa cgctaaatcc gatgaaacag agtaaaaaca
     5701 agccacgaat tcgccaggcg gttggagcca cccggcaatg tcgtaaacca caggcttaaa
     5761 cttcgacttg gtagcccgta tcttccagtg tgggattcat cgccgcggca cgagccagtt
     5821 catcacagcg ttcgttttcc gggtgtccgg catggccttt aacccattcc catttgattt
     5881 gatgctgccc caatgcagca tcaagacgtt gccagagatc gacatttttt actggttttt
     5941 tgtctgctgt tttccagcca cgttttttcc agttatggat ccactgggtg ataccctggc
     6001 ggacatactg gctgtcggta ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg
     6061 cgacaatagc ggccatcaac tccatacggt tgttggtggt gcgggtgtag cctgcgctaa
     6121 aggttttctc gcgtccgcga tagcgtaaaa tagcgccgta acccccaggt ccaggattac
     6181 ccagacacga accatcggtg aaaatttcta cctgtttaag catctctggt agacttcctg
     6241 taattgaatc gaactgtaaa acgacaagtc tgacataaat gaccgctatg agcactgcaa
     6301 ttacacgcca gatcgttctc gataccgaaa ccaccggtat gaaccagatt ggtgcacact
     6361 atgaaggcca caagatcatt gagattggtg ctgttgaagt agtgaaccgt cgcctgacgg
     6421 gcaataactt ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg
     6481 tacatggtat tgctgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg
     6541 agtttatgga ctatatccgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatag
     6601 gctttatgga ctacgagttt tcgttgctta agcgcgatat tccgaagacc aataccttct
     6661 gtaaggtcac cgatagcctt gcggtggcga ggaaaatgtt tcccggtaaa cgcaacagcc
     6721 tcgatgcgct atgtgcccgc tacgaaatag ataacagtaa acgaacgctg cacggggcat
     6781 tactcgatgc ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga
     6841 tggcttttgc gatggaagga gagacacagc agcaacaagg tgaagcaaca attcagcgca
     6901 ttgtacgtca ggcaagtaag ttacgcgttg tttttgcgac agatgaagag ctggcagcac
     6961 atgaagcccg tctcgatctg gtgcagaaaa aaggcggaag ctgcctctgg cgggcataaa
     7021 tacatgtaaa aggcgctaaa aatagcgact tgggcgattt ttgcagcaaa cgattcaaaa
     7081 gatgagaaaa atcgttgacg aaggtcgagg caatccgtaa tattcgcctc gttcccaacg
     7141 gaacacaacg cggagcggta gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc
     7201 gcgggttcga gtcccgtccg ttccgccaat attcagaaag cccgaatcag taatgattcg
     7261 ggctttcgtc gttttggtga agtgagaatt tttcactccg aagtaaaacg gatatgattt
     7321 aaaaaatggc cagacattat aaattctggc cacttctgtt actccagcgc atcccatttc
     7381 tccagcagtt ctactggaat ttcatttatt acctccgaga cgcgttttcc gaccaggcgt
     7441 tctgcctggc gcagcaaagg aatggtcggg ctgctcggtt cgctgtgctc aaaccagcga
     7501 cggatgacac gcaggcgttc cagcgcgtcg tttcggtcgc ggatctgccg gggttctgcg
     7561 ccggtcatcg gtgatatttc ctccgcgata tgcaccggta gcgcaatctg ctctgcctgc
     7621 atctgttcag tatgaacgat ggtggcgttt tccggctgct cagcctgtgg ttgtggcagg
     7681 atctccggcg tggtgctctg cactgctccc ggtaacaggg ccagcagttg cgtcagacgg
     7741 gagaagtccg gtgcctggtc attcagcgtt tcacgcgcat ggcgttgcag ccgctcagtg
     7801 gtctctgctg cctggcgaaa ggcatccagc ggcagtgtgc cgcgcgcttc caggtctgcc
     7861 agttgctggc gtaccgattc tggtgccagc gcatccgccg ggcgtaatgc agatagcgaa
     7921 cgctccacat cccgcacctg aaggcgcatg gcagcattat ttgacagtgt aatgccacga
     7981 atatccgcca ttacaccttc gtggtccagc aacgccgcca gcgcgttact gcgtgccacc
     8041 gcagcatctt cggcggtgat atcttcagtc gcgagcagtt gaggatggat ggcgtccggg
     8101 tacgtggagc acaacgtttc cagcagcgtg agcgcctccg acagtcccct ggcaccggcc
     8161 tgctggatac ggcagcgcag caggacaacc agaatgcgga tatctttgct gcgcatcagc
     8221 agacgatggg cgtcccgctc aagctccgcc cagttaatgt tttccggcgt actgacaaaa
     8281 tcgccatatt gcgcttcttc acgcggtgcg gcgcgggtga acagcagcag gtattccgga
     8341 tcgtactcca gatccgggcc gcaggggcat tccgccgaaa ttgggtcaga caggaatatt
     8401 tccatttaat tatcctcagt gcgccagcgt ggtggccaga ccgtccggat tgctggactc
     8461 acgcaccaga tgggccgatg ccgccagacg agcgtgatct ggcaacgggc tgtcagcgct
     8521 ctcccgcaaa tcctgcccgc tgaggctggc gacataaaag gcaaagcggt catgttgcgg
     8581 tttatccaga ccgcccccag cggactgggc gctgcgccat gcctggtaaa actgcgtcag
     8641 ccagcggtga tgaaaaagat cagaaaaacg ggtcagcgtc ggctttcagt ccggttacgc
     8701 gccagttcgg tgtagtgcag cggcagtgga ccgttcggcc cccacagcac gaggctgtac
     8761 agactgatgt gcaggcgccc ttcctgccag cccacactgg cgatttctcg tggcgcaaag
     8821 gtcagcgcgg gcgtttgtcc gaggcgaaat ttttccatcc gtggttgcca ggtgttgccc
     8881 gccggaacgg tacacaactg cacatccacg cggcgcatca ggttcagaaa accgtaacgc
     8941 cagggggggg cctgcgcctg agtgaaatcg ttgctcatac gcttccccgc tgtcccgccc
     9001 tgacaggcca ggtcataatt ttgccgcgct gcatgctgtg cagcgtcatc tgcgagaagg
     9061 tattaatgga aacatggcgg gcgatatagt gttccagcac caggccgaac agatacggac
     9121 tgatgccgga aaaaccttct tcatccaccg tcagttcaca actgacgcca cgcccataga
     9181 ccagtagtcc ggaaccgggc agacggcggg tgaccggggt cgttttacaa ccaatcaggc
     9241 tgcgcacctg gcgcgattgc ggactgtcat gagcaggaat aaacaggttc agcagatctc
     9301 gcaacgcctg gccgccggta cggtgatcca gatcggccag cggcagataa ttaaaagata
     9361 actggcggat caaccgccat gccatctcac gttcagccat cgggggctgc ggcgagcgtg
     9421 gcgggcggat aagccctacg cccgccaccg ggatggctgc atccacggtt agatcgtccc
     9481 ggccattgcg ggcaataaga cagggcagat cgcggttagt gaccatggcc gtgatggtga
     9541 tgtggcgcag attttccggg tacggcgctt catactggtc aaccagcgac aggaacactt
     9601 ccgagcccgt atagggggta cgggtgccgt aacggcgggc gttttctgat aagcggcggg
     9661 gctcccggcg cagggaaaaa taacgtccgt gattgccttc atcattattg cgggtgtggt
     9721 agagcgggcg aaaggccatc ttccgtgtgg tgtcggtttc cagcccttcc acttcctgga
     9781 ccgagaacac ctcataatcc agcggatggg ttctgtccac caccagatgc tgttccgtcg
     9841 tgctgtgcgt gacatcgatc cgggcggtga cgcggggaaa caggttgata accggggtac
     9901 agaacaggct gaactgtgcc gcatttgtct ggtggatcag ccagtccggc ggcaggcggt
     9961 tcagcaggat aacaatctcc gccacgccac cgtcaatttt ctgtaatccg gcagacaggc
    10021 ctgtcggggt gaaaaaatag aagcgttccg gacaggcaaa atattcatgc agcaggttat
    10081 gaccgtgaaa cacgttccac gccagcggca gcagcccctg atccggctcc aggccttcgt
    10141 acataacggg ctgttgcaga ttcacatcca gcgcgccgtc aaaatgcccc ggaatgccag
    10201 ccaggggcgc gatggcgctg gtatgtagaa gttctaacag gtgtgaggcc gtacgctcct
    10261 cgccgcagag ataaaacggc agcctgtcca gtccggcaag ctgactgaac gttagttcac
    10321 caaaggtgcg cagggtaata cgcagcgccc cggcgacgtg aatgttggcc ggcaggtagc
    10381 ggtgcagggc aggcatatcc ggtggcgcgg cggtcagtcg cgcctcttcg atggcgagcg
    10441 gccacagcgt tacatcctgg ctgctgcgaa actggcaggc agtgctttcg ccttccggaa
    10501 tggcggagaa aaaggctgtg tcgcgcggga cggtaaatcc tttcgccaga tcgccttctt
    10561 ccgtatcggg atgcaactgc gccacgctca ttgagggcgt gggtgtgacg taattggggc
    10621 tgaccacttc cagcaggcgc tgggtgaaac gggggaattc agcgtcaatt tttagctgcg
    10681 tgcgggcggt caggaagctg aaggcctcga ccattcgctc tacatacgga tcggcaatat
    10741 ccgttccctg catgcccagc cgggcggcga ctttgggatg acgggcggca aattcggctc
    10801 ccgtttcccg cagatagctc agttcgcggt tgtagtattc cagtagccgt ggatccatcg
    10861 gtctctctct gaattaaaaa aacgtaatgc gactctgctc catatccagc gcgctacgta
    10921 cccggaatgc agtcggatac ggctgcgtga ggatctcgcc gcgaatttca aactgtaatg
    10981 tgttatggcc actctggcgg cgctcatcct gaagtgggat gatttgcaga gtggacgctt
    11041 tcagccgggg ttcaaaccgg gtgatggttc gcaaaatggc cctgcggata tcgtcccact
    11101 tgtgctcata cagaaagctg cccgccagtg gcggcaggcc gtagttaagt acggacgctg
    11161 ccgcgtacgg ataacgtgca gcatcaatat catcttcatg gctgacggtg ttgagcagaa
    11221 atgacaaatc ccggcggatg atctccttaa tctgaaccgg actgacgctg ataccgtggt
    11281 cgcgtttctg gtttggggcg tcgtcacaca ggcggtcaaa cagcgtgggc agcaggtgat
    11341 tgcctggacg ctggcgggaa gcgctcacgc gtcgcttcct tcagcggtgt taaaggtaca
    11401 ggtggacaaa tccagcaggc tgatatcgcc gtgaccggtg agccacaccc tctgacccag
    11461 cgcctgtacc agtgtctctc ccggaccgtc ctgccagacc gtttcccggc acagacgcag
    11521 ggagtcggca gccttttcag agccgctgta gcggggaaac agctaggcac tgtgtgtatc
    11581 gccattgttc agggtgacgt tgacgggttt ccagatcaaa tcgatcagat gcgcaggccg
    11641 cggcgatttc agcgaacaga tttgtgaaaa cggcagccag atatatgctc cgcccgcgat
    11701 cagttcgagt accggaccgg tacgggagtc gctgtcgcta atccagtcaa atgcgccgcc
    11761 attccactgc ccggaagtgt ctgttatggc ctcaagcgcg cggctgcggt gagcatcagc
    11821 ttcctgcgct tcaccacggg cgttacaggc gagggcggtc agcagatcct ccatccaggc
    11881 gggcggcggg agaataacac cgggccgctg ttcgccctga aagcaggcgt ggcggtatat
    11941 ctcacagcgg atcagctcgc caaatacctg cgcttcgcga gtataatttg cgtccatccg
    12001 ggcgcatagc tggatctgtt gcagtgcccg cgaccagtcg cctgccacgc acaataactg
    12061 aaacaggctg tggcggcggg aggcctgcgc tgggtgcatc ctgacctgtt gctctgccat
    12121 ctgaatgccc tccaccacgg aataatcccg cagcagcgcc gacaaggtaa tcggtagctc
    12181 gttattgttt gtcatgatat atctctccat gttgtgccgg gtttatccgg taatggctgt
    12241 caatgccaat gatgcgatgc tctttccgtg tcagatctgg cagtacggtg ttatgcgctg
    12301 tttccggggc gtattccggg gaaagtaaat gcaggatatc agggggagtc ttttcgacag
    12361 gccagatagt ttcatcttcg ccagtggcat ccagtacatt gagaatagcg tcaatgctta
//
LOCUS       DAJTUP010000105        12184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTUP010000105 DAJTUP010000000
VERSION     DAJTUP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.105"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..602)
                     /locus_tag="OO468_003209"
     CDS             complement(<1..602)
                     /locus_tag="OO468_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6260219.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            1122..2021
                     /locus_tag="OO468_003210"
     CDS             1122..2021
                     /locus_tag="OO468_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6260220.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            2070..3083
                     /gene="yhjD"
                     /locus_tag="OO468_003211"
     CDS             2070..3083
                     /gene="yhjD"
                     /locus_tag="OO468_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR6260221.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3494..4816
                     /locus_tag="OO468_003212"
     CDS             3494..4816
                     /locus_tag="OO468_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR6260222.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4998..7058)
                     /locus_tag="OO468_003213"
     CDS             complement(4998..7058)
                     /locus_tag="OO468_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR6260223.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="OO468_003214"
     CDS             complement(7128..7895)
                     /gene="pdeH"
                     /locus_tag="OO468_003214"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR6260224.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            8127..9056
                     /gene="kdgK"
                     /locus_tag="OO468_003215"
     CDS             8127..9056
                     /gene="kdgK"
                     /locus_tag="OO468_003215"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR6260225.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(9152..10648)
                     /locus_tag="OO468_003216"
     CDS             complement(9152..10648)
                     /locus_tag="OO468_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6260226.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="OO468_003217"
     CDS             complement(10869..12155)
                     /gene="dctA"
                     /locus_tag="OO468_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR6260227.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2920 a         3310 c         3000 g         2954 t
ORIGIN      
        1 caggaggaga tatttaacat cattgccgcc tgggtgcgat ttttcacttc cagacggcga
       61 tacagggatt ccagatgcgc ttttaccgtt ccggtactga tattcagcgc tctgccgatc
      121 tccttatttg attcgcccgc cgctaacatg gttaaaatct cccgctggcg ggcgcttaac
      181 gatttgagat ctttaatgtc cttttccggc gtcgtccgcc agtctccagg cagaaacatc
      241 atccccatcg ccgcactatt taccgccaac gcaaatgtct cgacggttga atcgcgaggc
      301 acaatggcca gcacattaaa atggataact tcctgtaacc accgtttatt gcaatccgtc
      361 gccgtgatta acaccttaac ctcaggaaac tgcaccacgg ttttttgcag caaccagtag
      421 caaaactcac catcctgatc gccatcgagc ataactaagg cttcagggta actttccagc
      481 ttttgccata actcgtctgc ctgactgacc ccctgaatac tcactcctgg aatacgctgc
      541 tgtaaactga tttttattcc atgaataaat attgactgcc tgtcaaacat gactatttgc
      601 ataactgaat ctccacctga atacgttaaa aagacttaag tagtggaagg gtattacccg
      661 cgagaaaaaa taagaattcg ccatttggcg gtggccattc tacagagatg acgcgtagaa
      721 aatagttacc gatataaata gttacagcta aacgcctgaa attacatgtc gagggcacta
      781 tttaaaacaa ttttgaggat ttccttatat tggtggttag tacgcatgca attaaaaatg
      841 aaattccgcg actacaagcc aaaataacaa atggcaatga gacaaaaata agcacaaata
      901 gccaatacgt cctctattca ctttaaaggg aatcactgaa aaatacgctc tgtttaaggg
      961 gattcacctt tctcataaag ctattccgcc cttttcctgc tgagaaatcg ccacattcgt
     1021 catgacaaca ttgtgaaacc cggcattaga tgttagaaaa aacaataaac aatgcgatat
     1081 gcgcgttact gtttcgctgt gaacaacaat cggtcaaaga aatggataaa attcacgcaa
     1141 tgcagttgtt catcaaagtc gcggagctgg aaagtttttc ccgcgcggcg gatttctttg
     1201 ctttgccaaa gggaagtgtt tcgcgccaga tacaggcact ggaacatcaa cttggcaccc
     1261 agcttctcca gcgcactacg cgacgggtca aactcacgcc agaaggcatg acctattatc
     1321 aacgggcaaa agatgtgttg agtaatctca gcgaactgga cggtctgttt caacaggatg
     1381 ccaccagtat cagcggtaaa ttacgcatcg acatcccgtc aggaatcgcg aaaaacctgt
     1441 tactgccgcg tctgtcggaa tttctctatc agcatccggg aattgagctg gaactgagta
     1501 gccataaccg tccggtagat attcttcatg atggttttga ttgcgtgata cgcactggcg
     1561 cgttaccgga agatggcgtt atcgcccgtc ccctcggcaa actaaccatg gtcaactgtg
     1621 ccagtccgca ctatctgacg cgctttggtt atccgcaaag ccccgatgat ctgacttcac
     1681 acgcaatagt gcgttataca ccgcacctgg gtgtacatcc gttaggtttt gaggttgcca
     1741 gcgttaatgg cgtccagtgg tttaagtctg gcggcatgtt gacggtaaac agtagcgaaa
     1801 actatctcgc cgccggtctt gccggtctgg ggattattca gatcccgcgc attgccgtgc
     1861 gcgaagccct gcgtgccggg cggcttattg aagtgttgcc aggctaccgt gccgagccgc
     1921 tctccctttc gctggtttac ccgcagcgtc gggagctttc ccggcgtgta aacctgttta
     1981 tgcagtggct ggctggcgta atgaaagagc acctggactg accgactata ctttttaaga
     2041 atgaccacga cagaaggaca aaagagcgga tgacgcagga aaacgagatc aaacgtccca
     2101 cccaggatct ggagcacgag ccgattaagc agttggataa tagcgagaaa ggcggcaaag
     2161 ttagccaggc gctggaaacc gtcaccacca ctgccgaaaa agtccaacgc caaccggtta
     2221 ttgcgcacct tattcgcgcg acagaacgct ttaacgatcg gctgggtaac cagtttggtg
     2281 cggctatcac ctatttctca tttttgtcga tgatcccgat tttgatggtg tcgtttgccg
     2341 cagggggctt tgtgctggcc tcccatccga tgttgctaca ggatatcttc gacaaaattc
     2401 tgcaaaacat cagcgatccg acactagccg ccacgttgaa aaacaccatc aacaccgccg
     2461 ttcagcagcg tacgactgta gggcttgtcg gcctggcggt ggcactttat tccggcatca
     2521 actggatggg taacctgcgt gaagcgattc gtgcccagtc gcgcgatgtc tgggaacgct
     2581 cgccgcaaga tcaggagaag ttctgggtaa aatatctgcg tgattttatt tcactgattg
     2641 gtttgttgat tgcgctgatt gtgacgcttt cgatcacctc ggttgccggt tcggcgcagc
     2701 aaatgattat tagcgccctg cacctgaaca gcattgagtg gctgaaaccg acgtggcgat
     2761 tgattggcct ggcgatttcc atcttcgcca actatctgct tttcttctgg atcttctggc
     2821 ggttgccgcg ccaccgtccg cgcaaaaaag ccctgattcg cggaacattt ctggctgcta
     2881 ttggttttga agtgattaaa atcgtgatga cctacaccct gccatcgctg atgaaatccc
     2941 cctctggcgc agcatttggt tccgtgctgg ggctgatggc atttttctac ttcttcgccc
     3001 gtttgacgct gttttgcgcg gcgtggattg ccaccgccga atataaagac gacccgcgaa
     3061 tgccagggaa aacgcagcct taaaataggc cggatgcggc gtaaacgcct tatccggcat
     3121 acgattttat tcggcctggc tccccgtagg ccggataaga tgcgccagca tcgcatccgg
     3181 ctataatgcg cacataacct cttgaaactc ttccccagag cctcttcagc catctatttg
     3241 ggagcaaaca atttcactcc aactcataac cccagcatat aaatccagtt ggtaactttt
     3301 atttaacctg aaaccagttt tatccactat ttataaaatt atgtgaagca tttcatagaa
     3361 gaaaaatcac tggcctaaac attatcccct ttttgcctgg tttttgacca tttccgcgat
     3421 ttgttacaca ttgaaatatc acttttgctg tgcgtaatat ggctattcgt tagccaaaaa
     3481 ataagaaaag attatgcaag caacagccac aacactcgac cacgaacaag aatacacgcc
     3541 gatcaactcg cgtaataaag tccttgtcgc ctctctcatt ggcacggcca ttgagttctt
     3601 cgacttttac atttacgcca ctgcggccgt tattgtgttt ccgcatatct tcttcccgca
     3661 gggcgatcca acggcagcaa cgctacagtc gctcgccacc ttcgccatcg ccttcgtcgc
     3721 gcgccccatt ggctctgccg tttttggtca ttttggcgat cgcgttgggc gtaaagcgac
     3781 gctggtcgcc tcgttgctaa cgatggggat ttcgaccgtg gtgattggtc tgctgccggg
     3841 ctatgccacg attggtattt tcgccccact gctgctggcg ctggctcgat ttggtcaggg
     3901 tctgggctta ggcggtgaat ggggcggcgc ggcgctgctg gcgactgaaa acgccccacc
     3961 gcgcaaacgt gcactgtatg gctccttccc acagctaggc gcaccgattg gcttcttctt
     4021 tgccaacggc actttcttgc tgctttcctg gctactgacc gatgagcagt ttatgagctg
     4081 gggctggcgt gtgccattta tcttctcggc ggtgctggtc attatcggcc tgtatgttcg
     4141 cgtgtcgctg catgagtcgc cggtgtttga gaaagtcgct aaagcgaaaa aacaggtgaa
     4201 gatcccgctg ggtacgctgc tgaccaaaca tgttcgcgta accgtactgg gtacgttcat
     4261 tatgctggca acctatacgc tgttttacat catgacggtc tactctatga ccttcagtac
     4321 cgccgccgcg ccagttgggc ttggcctgcc gcgtaacgaa gtgttgtgga tgctgatgat
     4381 ggcagttatt ggttttggcg tgatggtgcc agtcgccgga ttactggctg atgcctttgg
     4441 tcgccgtaaa agcatggtga tcatcaccac gctgatcatc ctgttcgcgc tgttcgcctt
     4501 taacccgctg ctcggttctg gcaacccgat tctggttttt gccttcctgc tgctggggtt
     4561 aagtctgatg gggctgacct tcgggccgat gggtgcgctg ttaccagagc tgtttccgac
     4621 agaagtgcgt tacaccggag catcgttctc ttacaacgta tcgtcgattc tcggggcttc
     4681 cgttgcgccg tatatcgcag cctggttgca ggctaactac gggttaggtg cggtggggtt
     4741 atatctggcg gcgatggctg gcttgacgtt aatcgccctg ctgctgaccc atgagacgcg
     4801 acatcagtcg ttgtaataag tgtgaaaagc ctgatgcgct acgcttatca ggactgaatt
     4861 tccatacaat atattgaatt cacaaggatt tgtaggccgg ataaggcgtt cacgccgcat
     4921 ccggcatgaa caaagcacac tttgccaaaa atctaaatct gtttaattgc cggatgtcag
     4981 acatccggca attatcatca cttcttcatc tgcgacaata tcgtccgaca ctgattagcc
     5041 tccccttcgg aaggtgagat cagtgccagt aacgccgccg ccggtgttac cagcgttgcc
     5101 agtgccgcag caacagcacc acgggcaatc agcggtccgg ctttcacccc agcctgcgga
     5161 tttttaaacg tcccacgcac atacagcggc gaacgcagtg tgataatccg aatcccttta
     5221 ctctccggat caatagtcaa atccagctgt tccgaagcaa aacttgccgt gccggtaaca
     5281 ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg ggcgcgccac gccgttggca
     5341 atattcagat tcgccgccgc gcagttcacc cgcacctcat cgtcaccaaa tatcgcaccg
     5401 acaatgtagt tgccgacatt cagcccaaca atctccatca ggttgcggct caccagcccg
     5461 tcattcatca acagtttcag gttgccgtta ctgttgccta aaagcgccgc caccgagtta
     5521 ccgctaccgc gtagttccgc gtcaccgttc atttccccca gcgtcttctg catcagttcc
     5581 acatcgggca tcagttcttt cagtttcagt cgacgagcct gaatatctgc ccgcccctgc
     5641 atcggctttt tatcgccttc cagatgaata ttcgccgcaa tgctgccgcc cgccatgcca
     5701 aatttcagcg gttgcaggcg caggtcagca tttttgagga tgatatgagt agaaagatcg
     5761 ctaatcggca ggctactgcc atgctcaatg cgccgccctt tgaagcgaac atcggcatcc
     5821 ataacgtccc atttatcggt ttcgaagcgg tcataaggca gcactttgcc cgcaggctga
     5881 acgctttttt cgcccttctt ctgttcagac cgtttcgact tttctgcccc tttcccggaa
     5941 tcaacgccaa tcaacggtcc caggtccgcc agccgcaatt gccgcgactc gacatcacct
     6001 tccagttttg gtcgtggctt tccggtggtg tagaccagag aaccgtggat atcgctatcg
     6061 ccaattcgcc cattaaaacc gcgataatca aagaccgacg atttttcagt gtcgattttc
     6121 gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg gcagcagaac gcccgtcagt
     6181 tcatagagat cacccagtga atcgccagaa aatttaagcc gtaaatcgac accgcccatc
     6241 ttcattgggt cattcacgac gccatcaaaa gcaacacggg tattacctga acggaaatca
     6301 gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca gcataccgcc tatttttccc
     6361 gtacccgtga gcggttcacc gttataacgt ccctgcgcct tcaggccaaa aatgtaatcg
     6421 cccacctttt ctttatccgc tttacctttc gatccagtaa cttcgctgaa tggcagcggc
     6481 ttgcctaacg gatcaacaaa aatttccaga tccgctttgc ttactttgtc atcaatggcg
     6541 atccgccctt gatcgaaaag aatattatcc agccgaaacg accatgccga cggctttgca
     6601 ttcgcgtctt tgttgtcatc gttggcaaga ttaaacgtcc agttattgtt cttttcagag
     6661 aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc acggcagcca gaccgttttg
     6721 gtcagcagcg ccagcggtgc cagcgttgcc tctactcgcg gcaaatgcac catcgtgact
     6781 tcgggaatat ccggtggatt accaagaatg atgtcttctg catgtacatg aggccacggc
     6841 acccagctgc gccagccagt ttcttgtttt tgccgctccc acaccacgcc cagattgcca
     6901 cggatagcga acggacgatt caactccgca gagactttct ggttgatggt cggtttgagt
     6961 cgattccagt caaaagttgc aatcaaaatg atcgccacga caatcaacaa caagaaagcc
     7021 cctgaaatcg cagcggttat tttgcctgcc ttgctcatcg ttcgcttcct catattcttc
     7081 ctgtgccagt cctaaagata gtccagcccg gcggaaaatg aggcagctta tagcgccaga
     7141 accgccgtat tcagcgtttc catcggtgcc gggcgtgaaa gaaaccagcc ttgtgcggcg
     7201 aatgcgggcg agttctgaac atcacgccac tcttccggtg tttctacgcc ctcgacaatc
     7261 accccgcgac aatagcgatt cattagatgt aaaagctgag aaaagagtgt gcggccttcc
     7321 ggcgactgac gcagcatcac aaacagttct cgcgcgattt tgatgtagtc ataacgcact
     7381 tcacttagtg cagagaaatt tgccatcccg gtaccaaaat catccagcca cagcgggcca
     7441 aattcacaca tcgaggcaaa ggttgaatct ttcggcagac ggatatgctc caccagttcg
     7501 aaacgcagcc agggaagacg ctcaatctgg cgcaggattt ttggttgctg acgcagggcg
     7561 atgagcgtag ggccatcaat attgaccgat gccagcaggc cgtgctctac aaagaagtcg
     7621 gctttttgcg ccagcaaatc aatctgctct ttcacaacct ccatccgatg gctgacggtg
     7681 atttcagtaa aatagcgatc cggcggcagg cgttgcgaag ggttcaaggg atgcgtgacc
     7741 accgttaata gctccacggc cattaaccgc ccgcatgttt gatagatcgg ctgccaggtg
     7801 taagcacgct cacactgcaa ccaaaaacgc cgttcctgca agctctcgat gcttgcttca
     7861 gggttgctta ttcgctggat aacctgcctt atcatcaaag atgtcctgtg tttaaaagtg
     7921 atgctgcccg gactcgacaa agattatcgg cgcgtaaggg cagaacttta ccgcctggcc
     7981 ggggtaaatt tcctcactcc acaacggaat ctttcaggaa tacgttctgg ctcaaaaaaa
     8041 taatggaaca ctgttttaat atggttgacc agcaaaccac cacagcgcaa actaacgcta
     8101 attttttaca gatcaggttc acgactatgt ccaaaaagat tgccgtgatt ggcgaatgca
     8161 tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg tttcggcggc gataccctga
     8221 acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc attaaccgtt cattacgtaa
     8281 cggcgctggg aacggacagt tttagccagc agatgctgga cgcctggcac ggcgagaacg
     8341 ttgatacttc cctgacccaa cggatggaaa accgtcttcc gggcctttac tacattgaaa
     8401 ccgacagcac cggcgaacgt acgttctact actggcgtaa cgaagccgcc gccaaattct
     8461 ggctggcgag cgagcagtct gcggcgattt gcgaagagct ggcgaatttc gattatctct
     8521 acctgagcgg gattagcctg gcgatcttaa gcccgaccag ccgcgaaaag ctgctttccc
     8581 tgctgcgcga atgccgcgcc aaaggcggaa aagtgatttt cgacaataac tatcgtccgc
     8641 gcctgtgggc cagcaaagaa gagacacagc aggtgtacca gcaaatgctg gaatgcacgg
     8701 atatcgcctt cctgacgctg gacgacgaag acgcgctgtg gggtcaacag ccggtggaag
     8761 acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt ggtggtgaaa cgcggggcgg
     8821 attcttgcct ggtgtccatt gctggtgaag cgttagttga tgttccggcg gtgaaactgc
     8881 cgaaagaaaa agtgatcgat accaccgcag ctggcgactc tttcagcgcc ggttatctgg
     8941 cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa acgcgggcac ctgaccgcaa
     9001 gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga ggcgatgcca gcgtaaacgc
     9061 agcatatgca aaaggctgga taaagtgtta cccctctatc cagctcatcc ataaatacct
     9121 gatggttgtg ctaactcatc accatcaggc attactgtgc aggtggaata tccgtcactt
     9181 caggatgtac gtcatccgtg gcgacagagg tggtcgcagc ggcggtagat ggggccatga
     9241 tttgatccca ggccgcctgc aacgctttca tgttaaattc cggctcgcct ttcggctgca
     9301 gcagtattaa cgccatatca ttcgacaact gctgacgcag atcctgattt aacatctcca
     9361 cagtcaggct gttaaggaaa tcctgccgca gtttctgata ctgctccggc gcgatatcga
     9421 cgacctgatt ttgcaacgaa cgcatccgct ggcccatcag aatatcggta tcagcccgtg
     9481 cataggcggc aaacagtttc tgcagctcca gtttcttttg cgccactaac gcattgaact
     9541 cttcttccgg cagaccttta tcgcgaacct tcgccagttc acgcgccacc agattaaggt
     9601 tgctgttcag cttgtcgttt ggtgattcga tgttgatggc acactgcgca cgcagataca
     9661 gcacacggca gtcaaatcca agaccgatgt ctttgctgtc actggcgctt aacgcttgct
     9721 gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg ccagtagcgc agcagtgcgg
     9781 ctgattcacg aatcggctgc cacggcgtat cccacatgat agataaccgg tcctgacgca
     9841 ccgcgtcagt cataatactc accgcttccg cacgcagcgg agaaagcgtc ggcaccggag
     9901 ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt gttgatttgg tcgacaaccg
     9961 agcgcgcatc cacgtttccc accaccagta gcgtcattgc atccggggtg taccatttct
    10021 gatagaaatc tttaattttt tccgcttcaa cgggttgttt cagcggatcg gcaggatcgt
    10081 gacctaacaa ggttgaccct ttcagacgat agcgccacca gccctcttta gtatcggcag
    10141 gccaggttgc caccatgtcc tgactttgca gcgcgtggtt gatggtttct ggtgtgatgg
    10201 tcaatttgcc agtggcattt gccagataag agagcgcttc tttcagcaaa tcgttacggt
    10261 tattgggcaa actcagatta aacagcgtgg tgtcataaga gacaattacc ggcggcatcg
    10321 ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc ctgtgctgcg tcaaggccac
    10381 cgctttgcgt tagcgcaata cgagggatgg cgtgactgta accgctctgt tgtgtacttt
    10441 cggcgagcga accggtatta accagcaggc gaatttcaac acgatcgctg ggacgctggg
    10501 gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt cccctgttgc catgctggat
    10561 caggctggag cgcatctgcc tgcacatagc cagcagtggc catcatcagc aaaccgcccg
    10621 ctaaaagtcg aatttttatg ccctgcatgt gaacccctga tcaactatcc tggtaataaa
    10681 aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa cacttcgtgt tagaccgcaa
    10741 gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa ctcacccagg tggtaagttc
    10801 aggcacaggg gtcaattatg cgcaaacacc cgcactcggg gaagggagtg cgggcataag
    10861 tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg gcgcacgatt attcagcaca
    10921 tcgtccagtt ttttgtggtc cagttctttc acccacttag caacgacaat ggtcgctacg
    10981 ccgttaccga ccaggttagt tagcgcacga gcttctgaca taaagcggtc gataccgagg
    11041 atcagcgcca gacccgctac cggcaaatgg cccaccgcag agagcgtcgc cgccagcacg
    11101 ataaagccac tacccgttac ccctgccgcc cctttagaag aaagcagcaa cacgattaac
    11161 agcgtgattt ggtggacgat atccatctga ctgttagtgg cctgggcgat aaacaccgcc
    11221 gccattgtca ggtatatcga tgtgccatca aggttaaacg agtagcctgt cgggatgacc
    11281 agccccacca ccgatttacg gcagccgagt ttctccatct tgtcgagcat acgcggcagc
    11341 gccgactcgg aagatgaagt ccccagtaca atcagcagtt cttcacggat gtagcggata
    11401 aatttgaaga tactgaaacc agtcgcttta gcgattgaac ccaataccag caccacaaac
    11461 aggatacagg taatgtagaa acagataatc agctgcccca gttgcaccag tgtgccgacg
    11521 ccgtatttac cgatggtaaa cgccattgcc ccgagcgcac caataggtgc cagacgcatg
    11581 atcatattga tgatgccgaa gatgacctgc gagaaacttt cgatcacgtt aaaaatcagt
    11641 tggcctttgc tgcccagacg gtggagcgca aaaccaaaca gtacggcaaa cagcagcacc
    11701 tgcagaatgt taccgctggc aaatgcgcca atgacgctcg ccgggatgac atccataatg
    11761 aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa ccgctaccgc tttcgcatca
    11821 agcgttgccg gatcgacgtt cattccggca ccaggctgca cgacgttaac gatgataaga
    11881 ccaataatca gcgcgatggt actgacaatt tcaaagtaaa gcagtgcgac tgcgccggta
    11941 cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg ttacgacggt acaaaagatg
    12001 acaggagcga tgatcatctt aatgagctta acgaagccgt cgccaagcgg tttcatttgc
    12061 tcgcctattt caggatagaa atggccaagg agaataccaa tggctatcgc tgtcaggacc
    12121 tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt gtcctttagt aaaaccacag
    12181 gtct
//
LOCUS       DAJTUP010000106        11966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTUP010000106 DAJTUP010000000
VERSION     DAJTUP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.106"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            377..460
                     /locus_tag="OO468_003218"
     CDS             377..460
                     /locus_tag="OO468_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR6260228.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(514..1866)
                     /gene="gorA"
                     /locus_tag="OO468_003219"
     CDS             complement(514..1866)
                     /gene="gorA"
                     /locus_tag="OO468_003219"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR6260229.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(1938..2780)
                     /gene="rlmJ"
                     /locus_tag="OO468_003220"
     CDS             complement(1938..2780)
                     /gene="rlmJ"
                     /locus_tag="OO468_003220"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR6260230.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            2983..5025
                     /gene="prlC"
                     /locus_tag="OO468_003221"
     CDS             2983..5025
                     /gene="prlC"
                     /locus_tag="OO468_003221"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR6260231.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5033..5785
                     /gene="rsmJ"
                     /locus_tag="OO468_003222"
     CDS             5033..5785
                     /gene="rsmJ"
                     /locus_tag="OO468_003222"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR6260232.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(5834..7303)
                     /gene="dtpB"
                     /locus_tag="OO468_003223"
                     /pseudo
     CDS             complement(5834..7303)
                     /gene="dtpB"
                     /locus_tag="OO468_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7621..8055)
                     /gene="uspA"
                     /locus_tag="OO468_003224"
     CDS             complement(7621..8055)
                     /gene="uspA"
                     /locus_tag="OO468_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR6260233.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8449..8784
                     /gene="uspB"
                     /locus_tag="OO468_003225"
     CDS             8449..8784
                     /gene="uspB"
                     /locus_tag="OO468_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR6260234.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(8855..10354)
                     /gene="pitA"
                     /locus_tag="OO468_003226"
     CDS             complement(8855..10354)
                     /gene="pitA"
                     /locus_tag="OO468_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR6260235.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10586..11785
                     /locus_tag="OO468_003227"
     CDS             10586..11785
                     /locus_tag="OO468_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR6260236.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         2922 a         3063 c         3208 g         2773 t
ORIGIN      
        1 cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg gtttttttac tcttcagccc
       61 agagttggtg gctctggctg gagtgagaat gccatcatct aacgcatcaa tgctaatacc
      121 atactgaaac tattgcaagg ttatgctggt tatttagcct aattacactg tatgattatc
      181 cagttagctc tgaggcattt tcactctggc aatgcgcata aacgctttca aagtcctggt
      241 cagaagtacg ggtggtgccg ttaactgatg ctctggccgg agtgagattg cccacatcta
      301 acaatgaggc atgcgccgtg acaggcagtg gatgagtaag cggatgcatt ctcactccat
      361 cgcatggaga aaacgggtga ttgataaagc aatcatcgtt cttggggcgt taattgcgct
      421 gctggaactg atccgctttc tgcttcagct tctgaactga tagcggaaac gtaattaagg
      481 gctaagagca cactactctt agccctttaa catttaacgc atggtcacga actcttctgc
      541 cgccgttggg tgaatggcga cggtattgtc gaagtctttt ttggttgcgc ccatcttcag
      601 cgccaccgcg aagccctgca acatttcgtc cataccaaag ccaatgccgt gaataccgac
      661 aatcttctct tctggcccaa cgcacaccag cttcatgcgg cacggctggc ggtgagtggt
      721 gacggcggta tacatcgcgg tgaaagagga tttatacact ttcacctgat cgtcgccgta
      781 ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca atcggcggat ggctgaagac
      841 cacggtcgga atgttgctgt aatccagatg ctcatccggc ttgttattaa acaggcgttc
      901 agagagacgg cgacccgctg caactgccac cggtgtcagt tccactgcac ccgtgttatc
      961 gcccaccgcg taaatacctt caatattggt gttttgatat ttatcgacga cgatatagcc
     1021 tttttcgttg gttttaacgc cagcggcttc gaggttgatg ttgtcattgg caggctcgcg
     1081 accaatcgcc caaatcaggc aatccaccgt ttcactgcga ccatcttcca gctccagcgt
     1141 caggctacca tcggcatttt tcactaccgc tttcgggatg gcgttggtat gcaactgtgg
     1201 gccttcggcg ttcatcactt cgaccagcgt ttcagagatc atcgggtcga agctgcgcag
     1261 cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg aggccgttaa tcacgccagc
     1321 cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg cgctctggca aagcaggaag
     1381 ggcgaagaag ccatcggaat caataccgta ttccacgcct ggaatatccg ggtggctcgg
     1441 acgaccgccg gtggcgatca gaatatgatc ggccgtgatg gtttcgccgt taacttccag
     1501 cgttttggca tcaacgaagc gggcaaagcc tttgattaca tcaacgttat ttttaccgag
     1561 cacgttttca taggaagtat gaatacggtc gatataggcg gtacggctgg cgatcaacgt
     1621 ttcccagttg aacttgttga tggtggtatc gaaaccataa tcagggccgt acatatggat
     1681 cgcttcacgg atttgcgccg cgtgccacat cacttttttc ggcacgcagc cgacgttcac
     1741 gcaggtgccg cccagctctt tggcttcaat cagtgcgcat ttctggccgt acatagccgc
     1801 gcggttgatg gaggcgatac cgccgctgcc gccgccgatg gcgatgtaat catagtgttt
     1861 agtcatgaca tattgtcctt atcgttgatt accgcgattg tagcgcgaga cgtaataggt
     1921 gccagcaatg gctgcaatta ctccggcacg atccagctta cggtggcgtg cccggtacct
     1981 gccggaacca gtttgctgtg cagccacgac agcacgttat tcatctgctg ttccagcttc
     2041 cacggcgggt taatcacaat catgccggaa gcggtcatgc cacggcgatc gctgtctggc
     2101 agcaccgcca gttcaatttg cagaattttg cgaatgccgg tcgcttccag gtcgtggatc
     2161 atgcgcttaa tttgctggcg cagcaccacc ggataccaca atgcgtaagt gccagtggcg
     2221 aaacgtttgt aaccttctgc tatcccgctg accaccgcct gatagtcggt tttcatttca
     2281 tacggcgggt cgataaggat taaaccacgg cgagaaaccg gcggcagttt ggccttaagt
     2341 tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt ctttctgaaa tccagaacgc
     2401 agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca ggctgtcttg ttcgcgcagt
     2461 agctggcgag caatcaacgg cgaaccgggg tagtaacgca actggccgct acggttgaag
     2521 tgttttacca cattgatgta cgcctccagt tctgcgggca aatcgtcctg ctgccagata
     2581 cgggcgatac cttcgagata ttcgccggta cgctcggcat gttcgctacc taactgataa
     2641 cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt tatctttctc tttcagcgac
     2701 tcgatgatca ggctctgaac ggtatgttta aggacgtcgg cgtggttgcc agcgtgaaag
     2761 ctgtggcgat aactgagcat gggtaaaggt gttccggtaa gtaaaaattg gcccgtattc
     2821 ggggcgcaca aaagcctatc aggacagtat accgaaaaga ggccgccgcc gcgaaagcgt
     2881 aacgtttctc attgaaattc actacactta accccatgct acacacatta tgtaaagcgc
     2941 ctgttgagcg cttccttaac ctctttaacc aggactgcgc taatgacgaa tccgttactg
     3001 actccctttg aattgcctcc gttttctaaa attctcccgg aacatgtcgt tccagccgtg
     3061 actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg tagtagcgca aggggcaccg
     3121 tacacctggg aaaatctctg ccagccgttg gcggaagtgg acgatgtttt ggggcgtatc
     3181 ttctccccgg tcagccacct gaactcggtg aaaaatagcc cggaactgcg tgaagcgtac
     3241 gaacaaaccc tgccgctgct gtcggaatac agcacctggg tagggcaaca tgaagggctg
     3301 tataaagcgt accgcgacct gcgcgatggc gatcattacg ccacgctgaa cacggcgcag
     3361 aagaaagcgg ttgataacgc cctgcgtgat tttgaactct ctggcatagg tctgccgaaa
     3421 gagaaacagc agcgttacgg cgaaattgct acccgtcttt ctgaactggg caaccagtac
     3481 agcaacaacg tcctcgatgc gacgatgggc tggaccaaac tcgttaccga cgaagcgggg
     3541 ctggcgggga tgccagaaag cgcgctggct gcggcaaaag cccaggccga agcgaaagag
     3601 ctggaaggct acctgctgac gctggatatc ccaagctacc tgccggtaat gacctactgc
     3661 gacaaccagg ctctgcgcga agagatgtat cgcgcttaca gcacccgcgc ctccgatcaa
     3721 ggcccgaatg ccggtaagtg ggataacagc aaggtgatgg aagagatcct cgcgctgcgt
     3781 cacgaactgg cgcaactgct gggctttgaa aactacgcct ttaaatccct tgccactaaa
     3841 atggcagaaa acccgcagca ggtgctggat ttcttaaccg atctggcaaa acgcgcgcgt
     3901 ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg ctaaagccga atttggtgtc
     3961 gatgagttgc agccgtggga tatcgcttac tacagcgaaa aacagaaaca gcacctctac
     4021 agcatcagcg acgagcagct gcgtccgtac ttcccggaaa acaaagcggt taacggcctg
     4081 tttgaagtgg tgaaacgtat ttacggcatc accgctaaag agcgtaaaga tgttgatgtc
     4141 tggcatccgg atgtacgttt cttcgaactg tatgacgaaa ataacgaact gcgcggcagc
     4201 ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg gggcgtggat ggatggctgc
     4261 gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac cggtcgcgta tttgacttgt
     4321 aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca ctcacgacga agtgatcacc
     4381 ctgttccacg agttcggtca cggcctgcac catatgctga cccgcatcga aaccgctggt
     4441 gtttccggta tcagcggtgt gccgtgggat gcggtcgaac tgccgagtca gtttatggaa
     4501 aactggtgct gggagccgga ggcgctggcg tttatctctg gtcactatga aaccggcgaa
     4561 ccgctgccga aagagttgct ggataaaatg ctggcggcga agaactacca ggcggcgctg
     4621 tttattctgc gtcagctgga gttcggcctg tttgatttcc gccttcatgc cgagttccgc
     4681 ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa tcaagaaact ggttgccgtg
     4741 gtgccatctc cgtcctgggg ccgtttcccg cacgctttca gccatatttt cgccggtggt
     4801 tatgcggcag gttactacag ctacctgtgg gctgacgtac tggcggcaga tgctttctcg
     4861 cgctttgagg aagagggcat tttcaaccgt gaaaccgggc agtcgttcct cgacaacatt
     4921 ctgagccgtg gcggttcaga agagccgatg gatctgttca aacgcttccg tggtcgtgaa
     4981 ccgcagctgg atgcgatgct ggagcattac ggcattaagg gctgatcatt cagtgaaaat
     5041 ctgcttaatt gatgaaacag gcgccggaga cggcgcctta tctgttctgg cggcccgctg
     5101 ggggctggag cacgatgaag acaacctgat ggcgctggtg ttaacgccgg aacatctgga
     5161 attgcgcaag cgtgatgagc caaaacttgg cggcatcttt gttgattttg ttggcggagc
     5221 gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag gcggtggcga aagcggtggg
     5281 cattaaaggc gattatttgc cggatgtggt ggacgccacc gcagggctgg ggcgcgatgc
     5341 ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg gagcgtaatc cagtggttgc
     5401 cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat gcggaaatcg gcggctggtt
     5461 gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg gcgctaaccg atattacccc
     5521 acgcccgcag gtggtctatc tcgatccgat gttcccgcat aagcagaaaa gcgcgctggt
     5581 gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg gatcttgatg ccgatggatt
     5641 actggagcct gcacgcttgc tggcgaccaa acgcgtggtg gtaaagcgcc cggactacgc
     5701 gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg actaaagggc atcgctttga
     5761 tatctatgca ggtacgccag tgtaaaagta aagcagggta gcgataacgc taccctgctt
     5821 tctgcaagaa taattaatgg ctttccggcg tcgcaatcat gcgtttcagc cacggcacca
     5881 tcaacagcat cactactgca acgcccagcg tgaccagacc aatcttacca aacacgttgg
     5941 tatagacggg caacgtctca agcggatcgg taatgttgtc cggcaccgcg gtaaatgttg
     6001 ccacatagcc gcccagcaag aacgcggcag cctgcgtcag gaaccacatc ccgagaataa
     6061 agcccatcaa atgctgcggc accagggcag caatcatcgc caggccaagg gcgctaataa
     6121 acaattcacc caggctctag aataaataca ccagcacgat aaaccatggc gatgtcagcc
     6181 cctggtcatc cgcaaaccac attcccgcag cagcagccgt caaaaagccc agtgagcaca
     6241 taaacatgct gagagtaaat ttcatcggca tcgagaggtc tttgcctttg ttacccagat
     6301 gcgtgtaaat gcctgccagt attgggctgg cgagtaccac ccagaacggg ttaagcgcct
     6361 ggaagctgac cgggttgatg gaaaaaccga gaatttcatg atgcacgttg ttgatggcaa
     6421 agaagttcag cgatgttggc atctgggcgt agagaatgta aaacaccacc gcttcgagca
     6481 tcaggacaaa ggcgacaaac attttattgc gcccggtttt atccagcttg aatgcctgac
     6541 gaaagaagat gatagtgacg acgatggaga gaacaatcag caccagattg gcgacttcta
     6601 cgttgtgcat cagccatgca catacgaaga tcatcaccac gctgccaagt aatacgtaca
     6661 acagtttgct gaagctcatc ggcttgaagt cgggttcaga accaatgtct ttcaccattc
     6721 cacgacaggc gatgtaaacc agtaatgcga taattaaccc cgccccgcac aggttgtagg
     6781 tgactgaata accgaatcta tcagcgatca caggggccag cgataacgct atcaacgagc
     6841 cgatattgat cgacatatag aacagggtga atgcgccatc aagccgcgga tctttcggcg
     6901 gatagcactt cgaaagcaag ctggctgggt tagctttaaa caggccgtta ccgacagcga
     6961 tagtccccag ggcgatgaaa atcaggtcag gcttaagtag cgacatgccg gtcatgaagt
     7021 agccaatcgc cagcacaagt gctccaagaa caatggtgcg tttggtcccc aacaggtggt
     7081 cgccgacata gccgccaatg gaaatgaggc catagaccag tgcagcaaaa gcaccaaaag
     7141 tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac gaagaaaacc gccagtacgc
     7201 cctgcacgcc gtagtagccg aatcgctccc ataactcgac aaaaaagatc atgaaaaatg
     7261 ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt aatatttatt aaccttccaa
     7321 taccatcctg aaaagtatga acgcgtaagg gataacgcgc gaaaaagttt acatctgtca
     7381 gagattctta atattgatgc ggtgaataga ttaatacact attacaacag aaaataacca
     7441 gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat tttgtattgg caggagattt
     7501 tgattgcgta cgcagatcgt aggccagata aggggtttac gctgcatcag gtaacaaggc
     7561 gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg aacacgtcgt ggagggatga
     7621 ttattcttct tcgtcgcgta gcggaacaat cagcatatca acgtgaacgg tgttgatcag
     7681 ctgacgtgcg gaagacatca gtttgctcca gaagtcctgg tggtgaccac aaaccaccag
     7741 atccatatcg tatttcttga ttgcatcgac cagaacctgg cctaggtcgc cgctgccgct
     7801 cagggtttca gtgattggat agcctgcatt agtggaaagc tcggtcagcg catgatgtgt
     7861 ctcttcagag atgcgtttct gcatatcacc cagattcaca tcaataagcc cggtgtatag
     7921 gtcagagtag tttacatcta cgtggatcag agaaactttc gcattgtagg ggcgagccat
     7981 agagactgct ttctctacca gaactttgct ttccggggag agatcgactg cgatgagaat
     8041 gtgtttataa gccatagtgt tactccttcc ataaagttgt cgatgactgg ccagctagcg
     8101 tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg tgcgcggggc taacccagcc
     8161 gtaaagattc agtgttaaga ccatccttac cttatagcga cccggatgat ccgtcaatcc
     8221 gccttgctta cccgttagat aaacaaattt tcgacatgaa tattatattg atagtggtta
     8281 accttctgga aaaaaaacaa cctgatctcc tacactatct atagagccgc tcggatgttg
     8341 gtcaccacaa aagcggtttc tccaggacct ggtgttaact gattggcagc gtatcgggag
     8401 cggtagcccc ggggtggaac tccgcgggca ggtcgccggg gaggaggtat gataagcacc
     8461 gtcgcattat tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg ctatttctca
     8521 tcactacgcg cgttgttagt ggtactgcgt aactgcgatc cattgctcta tcaatatgtt
     8581 gatggagggg gcttttttac ctcacatggc caacccaaca aacaggtgcg tctcgtttgg
     8641 tatatctatg cccaacgtta tcgcgatcat cacgatgatg agtttattcg ccgctgtgag
     8701 cgggtgcgtc ggcagtttat tctgactagc gcattgtgtg gtctggtggt ggtcagcctg
     8761 attgcattga tgatttggca ttaagcactt cagcaataat aacgtataaa aaaagcgggc
     8821 cagtaaactg acccgctcgt tttggtgcgt acgattacag gaactgcaag gagaaccagt
     8881 acagcccgcc ggaaagtagt accgcagccg gaagggtaaa cacccaggcc atcagaatac
     8941 tggttacggt tttacgctgt aagccgccgc catctaccac catcgtcccc gcgacagaag
     9001 aggagagtac gtgagtggtg gaaaccggca tcccggtata actcgccagg ccgattgaca
     9061 ctgccgccgt catctgggca gacatcccct gagcgtaggt catgcctttc ttaccgattt
     9121 tctcaccgat agtcgttgcc acacgacgcc agccaatcat cgtaccgata cctaacgcca
     9181 gcgcgaccgc catgatgatc cacaccggcg catactcgat ggtgctaagc atgtcggact
     9241 tcagtttctt caacaggcgc tgatcgtcag cactcacacc aggcatcttc accactttgt
     9301 cgatagtgtc agaaacgcac agcataatgc ggcgcatctg gctacgttga tcaagcgaca
     9361 gcttgtcgta gctttccaca tcggtggtca acataccttt caggcggttg agcgcgttaa
     9421 tggtattcga cggatggcag tggaactccg caggttgcgt tgcgccagct tccggagccg
     9481 gtactaactg atcagcaccg gtagcctgtt tgagcagcgc aggatgctgc tcaaagtaag
     9541 cttcgacgtt gttgatggca tcacgggtac gggtgatttc gtagccagtg gcattcatgt
     9601 tcaccacgaa gcctgctggc gcgacgccaa tcaataccaa cataaccaga ccaatgcctt
     9661 tctgaccatc gttcgcgccg tgcgaaaacg ccacgccgat agcggaaagg atcagcgcga
     9721 tacgcgtcca gaacggcggc tttttcttgc cgtctttctt ttcacgctcc gctggggtca
     9781 ggtggatacg ggcgcgtttc ttggtgccgc tccagtagcg acgcagcaag aaaatcagac
     9841 cgccagcaaa caccaggccg acaatagggg aaacgatcag agaaccgaaa atacttaata
     9901 ctttcgggat attgagtgca tccaccactg acgtcccggt catcaacgca ttggttaaac
     9961 caatcccgat gatcgcgcca atcagcgtat gagagctgga tgcaggtaaa ccaaagtacc
    10021 aggtacccag gttccagata atcgccgcca gcaacataga gaacaccatg gcaaggccat
    10081 gagacgatcc catattaagc agcagatccg tcggcagcat atgcacaatg gcataggcaa
    10141 cactcagacc acccagcaaa acacccaaaa agttgaacac cgccgccata accacggcga
    10201 gctgagaacg catcgcgcgg gtatagataa cggttgccac ggcgttggct gtgtcatgga
    10261 aaccattgat ggcttcgtag aacagcacaa aagccagtgc aagcaataat aacagcccgg
    10321 tatgcaaatc caggccagca aacaaatgta gcataggacg ttacgccatt ttgaggacat
    10381 gaacgcggcg cattatcagt gactttcgcg gcgcgggcaa agtgaaatat agactttttt
    10441 tgatttgcct cctgtatgga tttcactcaa aaaataatta tcttatataa ttcaggcaaa
    10501 tacttccttt tagtaatatt gatgctggtg cgaccactga ggaatcttta caattcacgc
    10561 ccgttttttc taagaggagc gcaacgtgga aaggtttgat gccattatta taggcgctgg
    10621 tgcggcgggt atgttctgtt ctgcgctggc aggtcaggta ggacgccggg ttctgctgat
    10681 cgataatggt aaaatactag ggcgcaaaat ccttatgtct ggcggtgggc gctgcaactt
    10741 taccaacctt tatgtcgaac caggcgctta tctgagccag aatccgcatt tttgtaagtc
    10801 tgcactcgca cgttttaccc agtgggattt cattgatctg gtcaataaac acggtatcgc
    10861 ctggcacgag aaaacgttag ggcaactctt ctgcgatgac tccgcgcagc agattgtcga
    10921 catgctggtg gatgagtgcg agaagggcaa tgtaaccttc agattgcgta gcgaagtgct
    10981 gagtgtggcg aaggatgaaa caggcttcac gcttgatctg aacggcatga ctgtcggttg
    11041 cgaaaagctg gtcatcgcga ccggtgggct gtcaatgccg gggctgggcg catcgccgtt
    11101 tggttataag attgccgaac aatttggcct caacgtgctg ccgacccgcg cgggtctggt
    11161 gccattcact ctgcataaac cgttgctcga agagttacag gtgctggcgg gcgtggcggt
    11221 gccttccgtg attaccgctg aaaacggcac cgttttccgt gagaacttac tcttcaccca
    11281 ccgcggctta tctggaccgg cggtgttgca gatttcaagc tactggcaac cgggtgaatt
    11341 tgtcagtatc aatctgctac cggatgtgga cctcgaaacc ttcctgaatg agcagcgtaa
    11401 cgcacatccg aatcaaagcc tgaaaaacac actggcggtt catctaccga agcggttggt
    11461 tgaacgctta cagcaactcg ggcaaatccc ggatgtttcg ctaaaacagc tcaacgtgtg
    11521 tgaccaacag gcactgatta gcacattgac cgactggcgc gtacaaccca acggcactga
    11581 aggctatcgc actgccgaag tgacgctcgg cggcgtggac accaacgaac tttcttcacg
    11641 gacgatggaa gcgcgcaaag tgcctgggct gtacttcatc ggtgaagtga tggacgttac
    11701 cggctggctg gggggctata acttccagtg ggcgtggtcg agtgcatggg cttgtgcgca
    11761 ggatttggcg tcaattgtga aataaccatg acgttttccc gtcagccgtt tgtatatgtc
    11821 gttattaagt cattttggta cggttctgca tccgacattc tggtgttaac tcgctgtttt
    11881 gttagcgaat taacgctatt gccgttgtgg ttattttata gtgtatttat tctcagagaa
    11941 tatatattac tgaaataaaa aacaca
//
LOCUS       DAJTUP010000107        11860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTUP010000107 DAJTUP010000000
VERSION     DAJTUP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.107"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            110..1807
                     /gene="maeA"
                     /locus_tag="OO468_003228"
     CDS             110..1807
                     /gene="maeA"
                     /locus_tag="OO468_003228"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR6260237.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1941..2951
                     /gene="adhP"
                     /locus_tag="OO468_003229"
     CDS             1941..2951
                     /gene="adhP"
                     /locus_tag="OO468_003229"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR6260238.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3097..3381
                     /locus_tag="OO468_003230"
     CDS             3097..3381
                     /locus_tag="OO468_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR6260239.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3609..4262)
                     /gene="fdnI"
                     /locus_tag="OO468_003231"
     CDS             complement(3609..4262)
                     /gene="fdnI"
                     /locus_tag="OO468_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR6260240.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4255..5139)
                     /gene="fdnH"
                     /locus_tag="OO468_003232"
     CDS             complement(4255..5139)
                     /gene="fdnH"
                     /locus_tag="OO468_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR6260241.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5152..8199)
                     /gene="fdnG"
                     /locus_tag="OO468_003233"
     CDS             complement(5152..8199)
                     /gene="fdnG"
                     /locus_tag="OO468_003233"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7612..7614),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6260242.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQSLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8431..9312
                     /gene="yddG"
                     /locus_tag="OO468_003234"
     CDS             8431..9312
                     /gene="yddG"
                     /locus_tag="OO468_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR6260243.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9567..10667
                     /gene="ompC"
                     /locus_tag="OO468_003235"
     CDS             9567..10667
                     /gene="ompC"
                     /locus_tag="OO468_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR6260244.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10837..11316
                     /locus_tag="OO468_003236"
     CDS             10837..11316
                     /locus_tag="OO468_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6260245.1"
                     /translation="MTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKSVN
                     NGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADN
                     ASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            11426..>11860
                     /locus_tag="OO468_003237"
     CDS             11426..>11860
                     /locus_tag="OO468_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR6260246.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESIN"
BASE COUNT         3050 a         2978 c         2833 g         2999 t
ORIGIN      
        1 ctgcgcaatg gtgtttttat ctgctttata cttggggacg acgccctggc ggtaaagcaa
       61 agacgataaa agcgtgccag ggatggatat tcaaaaaaga gtgagtgaca tggaaccaaa
      121 aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc cctgtactgc tggaatttcc
      181 gttgttgaat aaaggcagtg ccttcagcat ggaagaacgc cgtaacttca acctgctggg
      241 gttactgccg gaagtggtcg aaaccatcga agaacaagcg gaacgagcat ggatccagta
      301 tcagggattc aaaaccgaaa tcgacaaaca catctacctg cgtaacatcc aggacaccaa
      361 cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat gagatgatgc ctgttattta
      421 taccccaacc gtcggcgcag cctgtgagcg tttttctgag atctaccgcc gttcacgcgg
      481 cgtgtttatc tcttaccaga accgccacaa tatggacgat attctgcaaa acgtgccgaa
      541 ccataatatt aaagtgattg tggtgactga cggtgaacgt attctggggc ttggtgacca
      601 gggcatcggc gggatgggca ttccgatcgg taaactgtcg ctctataccg cctgtggcgg
      661 catcagcccg gcgtataccc ttccggtggt gctggatgtc ggaacgaaca accaacagct
      721 gcttaacgat ccgctgtata tgggctggcg taatccgcgt atcactgacg atgaatacta
      781 tgaattcgtt gatgaattta tccaggctgt gaaacaacgc tggccggacg tgctgttgca
      841 gtttgaagac tttgctcaaa aaaatgcgat gccgttactt aaccgctatc gcaatgaaat
      901 ttgttctttt aacgatgaca ttcagggcac tgcggcggta acagtcggca cactgatcgc
      961 agccagccgc gcggcaggtg gtcagttaag cgagaaaaaa atcgtcttcc ttggcgcagg
     1021 ttcagcggga tgcggcattg ccgaaatgat catcgcccag acccagcgcg aaggattaag
     1081 cgaggaagca gcgcggcaga aagtctttat ggtcgatcgc tttggcctgc tgacggacaa
     1141 gatgccgaac ctgctgcctt tccagaccaa actggtgcag aagcgcgaaa acctcagtga
     1201 ctgggatacc gacagcgatg tgttgtcact gctggatgtg gtgcgcaatg taaaaccaga
     1261 tattctgatt ggcgtctcag gacagaccgg gctgtttacg gaagagatca tccgtgagat
     1321 gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct aacccgacgt ctcgcgtgga
     1381 agccacaccg caggacatta tcgcctggac cgaaggtaac gcgctggtcg ccactggcag
     1441 cccgtttaat cctgtggtat ggaaagataa aatctaccct atcgcccagt gtaacaacgc
     1501 ctttattttc ccgggcatcg gcctgggtgt tattgcttcc ggcgcgtcac gtatcaccga
     1561 tgagatgctg atgtcggcaa gtgaaacgct tgctcagtat tcgccgctgg tcctgaacgg
     1621 cgaaggtctg gtactaccgg aactgaaaga tattcagaaa gtctcccgcg caattgcgtt
     1681 tgcggttggc aaaatggcgc agcagcaagg cgtggcggtc aaaacgtctg ctgaagcttt
     1741 gcaacaagcc attgacgata atttctggca agccgaatac cgcgactacc gccgtacctc
     1801 catctaagcc tgcacccggt agtgaaggct accgggctat ttccctctcc ctttttcaga
     1861 tctcatccat actgggtagt ggcgaataaa tctcatttgc ctcacctgct atgcagaaca
     1921 tcatccgaaa aggaggaact atgaagactg cagttgttac gaaggatcat catgttgacg
     1981 ttacggataa aacactgcgc tcactgaaac atggcgaagc cctgctgaaa atggagtgtt
     2041 gtggtgtatg tcataccgat cttcatgtta agaatggcga ttttggtgac aaaaccggcg
     2101 taattctggg ccatgaaggc atcggtgtgg tggcagaagt tggcccaggt gtcacctcat
     2161 taaaaccagg cgatcgtgcc agcgtggcgt ggttttacga aggatgcggt cattgcgaat
     2221 actgtaacag tggtaacgaa acgctctgcc gttcagttaa aaatgccgga tacagcgttg
     2281 atggcgggat ggaggaagag tgcatcgtgg tcgccgatta cgcggtaaaa gtgccagatg
     2341 gtctggactc ggcggcgccc agcagcatta cctgtgcggg ggtcaccacc tacaaagccg
     2401 ttaaactgtc aaaaattcgt cccggtcagt ggattgctat ctacggtctt ggcggtctgg
     2461 gtaacctcgc cctgcaatac gcaaagaatg tctttaacgc caaagtgatc gccattgatg
     2521 tcaatgatga gcagttaaaa ctggcaaccg aaatggatgc agatttagcg attaactcac
     2581 gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg tggcgctcac gctgcggtgg
     2641 taacagcagt agctaaagct gcgtttaact cggcagttga tgctgtccgt gcaggcggtc
     2701 gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct ggatatccca cgtcttgtgc
     2761 tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg ccaggatcta accgaagcct
     2821 tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc cctgcgtccg ttagcggaca
     2881 tcaacaccat ctttaccgag atggaagaag gcaaaatccg tggccgtatg gtgattgatt
     2941 tccgtcacta agaggtcttt gctgcgactc ccatgttcgg gtcgcagcat cgcacactct
     3001 ccaacatgaa atggctaaat ggattattgt tgcgtaggaa ggaacaataa tgatagtgtt
     3061 cccagagaag taacgctact gagagtaaag acactaatga aaatggccaa tcatccccgc
     3121 ccgggggaca ttattcagga atcactggac gaacttaatg tcagcctgcg cgagtttgcc
     3181 agagcaatgg aaattgcgcc ctcaacggca agccgattgc tgaccggaaa agcagctttg
     3241 acgccagaaa tggcaattaa actttccgtg gtgatcggca gttcgccgca aatgtggctg
     3301 aatctgcaaa acgcctggag tctggcagag gcagaaaaaa cggtggttgt gtcgcgatta
     3361 cgccgtctgg ttacgcaata aaaaatggca ccctataaag cgccatttga aactgatgac
     3421 aaacgcaaca ttgcctgatg cgctacgctt atcaggccta cacgttcact tcaatctatt
     3481 gtatttgaag ggttttcatg ccggataagg cattcacgac gcatccggga tgaacaaagc
     3541 ccacgttgtc tacaatatga aaatggcgcc ttttacagcg ccattttcag cttaaagttc
     3601 aaaagagatt atatcccttc ttcactctct tttttcgctt ctgccttctc gatttcacga
     3661 taccagcgcg gatggtgttt cttcgcccag cgacggctca ccttcccttc gatcatccct
     3721 ttaatcgacc ctttcaccca gaatgccata tacatatgga tcaggatggc gtggatcagg
     3781 atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa caacctgcat cgggaagtac
     3841 tgcgcaaagt acggacgcca gataatcacc ccggtcacca gcagcacgaa aatcatgctc
     3901 atgatcgacc agaacatcat cttttgcccg gcgttgtact taccaacatc cgccactttg
     3961 tgctcattgc cttttaatac ttcgacaatg ttcaacagcc acggaatatc tttcttatcc
     4021 gggatgttgt gatgcacaaa acgcacaaac ataaacatca gtgcgacgaa aatcgcaatg
     4081 ccgaagaagg ggtgcaaaat gcgtcccatc tgcggcgtac cgaaggtttg cgtcagccat
     4141 tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca ccaggaagaa gcaaatcacc
     4201 acggtccagt gacaggcgcg atcaataaat ttggtgcgca caatcatttt cgacttactc
     4261 atgatgatcc tcctcgtcat cgtccacttc cttattcggg ccaataccga tgtagtggaa
     4321 aatcaacccg gcaaaagtgg caataaagcc agccgctgcc agcggtttca acgcgccttt
     4381 ccacaggctt accgaggtgt cgatcttcgg atctttcggc agaccgtgat acagctccgg
     4441 ctgatcggcg tgatgcagca cgtacataac gtgcgtacca ccgacccctt ccgggttgta
     4501 gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc acgcgctgtt ccgccagctc
     4561 cagcatctcc ttcttggtgc cgaagtggat agccccggtc ggacaggttt tcacacaagc
     4621 cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg catttatata cccggttatc
     4681 ctctttgttg aggcgcggaa tattaaacgg acacccggca atgcagtaac cacagccgat
     4741 gcagttttcc gactggaaat cgacaatccc gttagcgtac tgaatgattg caccagcaga
     4801 cgggcacgcc ttcaggcagc ccggatcttc acagtgcata cagccgtctt tacggatcag
     4861 ccactccagc ttgccattct gttcggtttc gctaaagcgc atcaccgtcc aggacttggc
     4921 gctcagatcg gcggggttat cgtaaacccc gacgcagtgc cccacttcat cacggatgtc
     4981 gttccactcc gaacacgcca cctgacaggc tttacagccg atacaggtgg aaacgtcgat
     5041 aagtttcgcg acttctgctt tgtaatcacg cacctgagaa ggcggcgtga tggagttagt
     5101 tgcggacctt ttgataatat cctgcgtttc catagccatc tgttcgcccc cttacgcctt
     5161 ctcgatgttg actaagaacg ctttatattc cggcgtttgc gagtttgcat caccgacatt
     5221 cggcgtcaga gtgttagcga tataaccttt acgcgcgaca ccctcaaagc cccagtggat
     5281 tggaataccc accgtttcaa cctgctgacc gtttacattc agcggtttca gacggcgcgt
     5341 taccactgcc accgcgcgga taaagcctcg cttgctggag acagtgacac gatcgccatt
     5401 attaatgcct ttcgccgccg cctgcgtttc gctgatttcc acaaactgtt ccggctgagc
     5461 aattgcgttg agcaacgcgt gcttggtcca ggtgtggaag tgctcggtca gacgataggt
     5521 cgtacccaca tacgggaact gctctttttt acccatccgc agcgcgtctt gttcatacag
     5581 acgaacaacc gggttagaca ccacgttcgg atgcagcggg ttagtgccca gcggcgtttc
     5641 aatcggctcg tagtgttccg ggaacggacc ttccgccatt ttgttgatgg caaacaggcg
     5701 tcccatccct tccggctgca taataaacgg cccggttggc gtacccggtg cggcattgcc
     5761 gaagtccgga atatcgttac ccgtccactt gctgccgttc cactggatca gcatccgttt
     5821 cggatcccac ggtttaccgt tgatatccgc cgaagcacgg ttgtagagta cgcgacggtt
     5881 gagcggccac gcccaggccc atcccagcgt attccccaga ccggacgggt cggagttatc
     5941 gcggtttgcc atctggttac cctgctctgt ccagctaccg gtgtagatcc agcaagaaga
     6001 tgcggttgta ccgtcatcac gcagatgcgc aaagctactc agcaactgac ctttcttcgc
     6061 aatcagcacg ccattagcat catagagatc ttccaacgca tagccgttgt tctctttagc
     6121 tacttcgtca gattgcggtt cgtgcggctg cttgtagttc cagctcatct tcatcagcgg
     6181 ttctacgcct ttaccacctt cggcctggta caactcgcgc agatgatggt agatacccgc
     6241 cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc tgacctttcc agtgccactg
     6301 caaccagcga ccggagttag cgatagaacc atcctcttca gcaaagcagg tcgaaggcag
     6361 acggaatact tcagtctgaa tagacgccgg atcgacatcg ttcgactcgc cgtggttctg
     6421 ccagaaggta gaggtttcag tcaccagcgg atcgataacc accatgtact tcagtttgct
     6481 caggcagctc accactttgt ttttatccgg gaaggacgca accgggttaa agccctggca
     6541 gaaataaccg gtaactttgc cttcatccat catgttgaaa tacttgatga cgtcgtaggt
     6601 ctggtcccac ttcggcagcc agtcatagcc ccagttgttc tctttctgcg cggcatcgcc
     6661 atagaaagat ttcatcaggc taacgaagaa cttcggatag ttgctccagt agttcacctg
     6721 atcagccagc gtcgctttcg gcgtgttcgc ttccagatac gactgcaaat caacctgttt
     6781 ttctgacggc agcgtcagat aacctggcag gctggtagag agcagaccta agtcagtcag
     6841 cccctgaatg ttggagtgac cacgcaatgc gttcacgccg ccaccggcca tacccatgtt
     6901 accgagcagc gactggatca tcgccatagt acggatgttc tgcgcaccca cggtgtgctg
     6961 cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc ggtgcgctgg tggaggccag
     7021 cacttcacac actttcagga agtcggcttt tggcgtacca cagatgtttt caacaacgtc
     7081 cggcgtgtag cgggaaacgt gcgctttcag caggttccac acacagcgcg gatgagtcag
     7141 tgtttcatcg cgtttcgcat agccgttttc atcgaactga tagttccagg acgatttgtc
     7201 gtactggcgt ttttcagcgt cataaccgct gaacaggcca tcttcgaaag caaaatcatc
     7261 acgcaccagt aggctggcgt tggtgtaatg tttaacgtat tcggcgttga ttttgttgtt
     7321 ttcgatcagg tagcgcaaaa tgccagacag gaacgtaatg tccgtaccgg aacgaatagg
     7381 tgcgtaaata tccgccacag aagcggtacg cgtaaaacgg ggatcgacaa cgatcaaggt
     7441 tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa ccgacgggat gcgcttcagc
     7501 agcgttaccg cccattacca ttacgacgtt agcgtttttg atatccaccc agtggttggt
     7561 catagcaccg cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacgcg
     7621 cgcctggttg tctaccgcca gcatcccgag ggagcgggca aatttctggg tcagcatccc
     7681 ggtttcgttg ctggcaccgg aggcacacag cataccggta gaaagccaac ggtttaccgt
     7741 tacgccctgc tcgttctttt caataaagtt agcgtcacgg tcagctttca tcagcttcgc
     7801 aatacgggag aatgcttctt cccagctaat gcgctgccat ttgtcagaac ctggcgcacg
     7861 atatttcggg tagcgcagac ggttttcact gttgacgtaa tccagcaaac cggccccttt
     7921 cgggcacagc gcaccacggc ttaccggatg atccgggtca ccttcaatgt gataaatcgc
     7981 ttctctggcg ttttttgcgc catcacccag gctatacatc aatagcccgc aacctacgga
     8041 acagtatgtg caggtgttac ggatctcttt agcgcgtaat aatttgtagt ttcgcgcctg
     8101 agccagtgct tgcttcgggg caaagcccaa tgccgctacc gttgttccag ccataccgcc
     8161 cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt gctttcctct tttttcaggg
     8221 gtattactgc gcgaggaaac taacagtaaa attctgaatc tttttgcgcg aaatcaaaaa
     8281 gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt gacctacctc aatagcggta
     8341 gaaaaacgca ccactgcctg acaggccagt taaaaaaatg ctataaaatt cagcttaatt
     8401 tttaacggca agagagacaa aacagcgagc atgacacgac aaaaagcaac gctcataggg
     8461 ctaatagcga tcgtcctgtg gagcacgatg gtaggattga ttcgcggtgt cagcgagggg
     8521 ctcggcccgg tcggcggcgc agctgctatc tattcattaa gcgggctgct gttaatcttc
     8581 acggttggat ttccgcgtat tcggcagatt ccgaaaggct atttactcgc cgggagtctg
     8641 ttattcgtca gctatgaaat ctatctggcg ctttccttag ggtatgcggc gacccgtcat
     8701 caggcgattg aagtgggtat ggtgaactat ctgtggccca gcctgacaat tctctttgcc
     8761 attctgttta atggtcagaa aaccaactgg ctgattgtac caggattatt attagccctg
     8821 gtcggcgtct gctgggtgtt aggcggtgag aatgggttac attatgatga aatcatcaat
     8881 aatatcacca ccagcccatt gagttatttc ctggcgttta ttggtgcgtt tatctgggca
     8941 gcctattgca cagtaacgaa taaatacgca cgcggattta acggaattac cgtttttgtc
     9001 ctgctaacgg gagcatgtct gtggatttac tattttctca cgccacaacc agaaatggta
     9061 tttagcacgc ccgtcatgat taaactcatc tctgcggcat ttaccttagg atttgcttat
     9121 actgcatgga atgtcggtat attgcatggc aatgtcacca taatggcggt aggctcgtat
     9181 tttacgcctg tactttcctc agcgcttgca gccgtgctgc tcagcgcccc gctgtcgttc
     9241 tcgttctggc aaggcgcact aatggtctgc ggcggttccc tgctctgctg gctggcgaca
     9301 cgtcgtggtt aaaataattg ccgggtctac ggcccggcag ttaatcagat aaattccgac
     9361 aatattaaaa aataaatttc aaataccagt aattcacctt atatctcatt tcgacttcga
     9421 tcacatatcg ataaataata ttattctatt tttctcattg tttttaatca ccattctatt
     9481 ttaaaaacag tatgtcattt ataaatgacg ccgtttaaaa ttcgtttaga aaatacgttt
     9541 aacgataatt aaaaggattc tgttaaatga aattaaaaat agttgcggtg gttgtaactg
     9601 gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta taacaaggat ggtaataaac
     9661 tcgaccttta tggcaaggtt accgctctac gttattttac tgatgataag cgtgacgatg
     9721 gtgataaaac ttatgcccgt ctcggcttta aaggagaaac gcaaatcaat gatcaaatga
     9781 ttggttttgg tcactgggaa tatgatttta aaggctataa cgatgaagcc aacggctcgc
     9841 gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat tagtgaattt ggctctctgg
     9901 actatggtcg taactacggt gtcggctacg acattggttc atggaccgat atgttgccag
     9961 aatttggtgg cgatacctgg agtcagaaag atgtcttcat gacataccgt accaccggtc
    10021 tagcaaccta tcgcaactac gatttctttg gcttaattga agggctgaac tttgccgcgc
    10081 aatatcaagg caaaaataaa cgtactgaca acagtcatct ttatggtgct gacgacacgc
    10141 gtgccaacgg tgacggtttc ggtatctcct caacttatgt ttatgatggc tttggtatcg
    10201 gtgcggtata taccaaatcc gatcggacaa atgcgcagga aagagccgca gctaatcctc
    10261 tcaatgcctc cggtaagaat gcagaactat gggccacagg tataaagtat gatgccaaca
    10321 acatctactt tgcagctaat tacgatgaaa cattaaacat gaccacctat ggggatggtt
    10381 atatctctaa caaagcacaa agttttgaag tggtggcgca atatcaattc gacttcggct
    10441 tgcgcccatc actcgcttac ctgaaatcga aaggcagaga tctgggccgc tacgccgacc
    10501 aggacatgat tgagtatatc gacgttggtg cgacgtattt cttcaacaaa aatatgtcga
    10561 cctatgttga ttataaaatc aacctgattg atgaaagcga ctttacccgt gccgtagata
    10621 ttcgcaccga taacatcgtc gctacgggca ttacctatca gttctaacgt cctctcctga
    10681 ccagcccttc tctgggctgg tttttgattt aagtgctgct gtatgactcc ttaagtaaaa
    10741 atgagcaatt cttgatattt ctggtcattc acctcaatta atattattcc atatcctccg
    10801 gggacatctt actataatat ttattgatga gtgattatga ccaatattaa tacagcttgt
    10861 gtaaaaaata atgccagtta tcaacttaat aacgcattac ctaacaaaga aaccatctcc
    10921 agcaattttt gtgaacgact agaacaatgg ggtaataaat cggttaataa tggtgaagaa
    10981 agagcaattg ccgtggagcg aattaaagaa gcttacaatt cgaacatggc atctcttgat
    11041 ttgtcatatc ttgatttgag tgaattaccg cctatcccct cgacagttaa tacgttaaat
    11101 ctggaaaaca attgtctaac ttgtcttgac ttagctgaca atgccagtct cgttaatatc
    11161 aacctcagcg ttaataaaat taacacgata acctttccaa aagaaccaaa actggaaaac
    11221 atttatatta atcataacaa tctggaaagt ttagatttaa aaaatcagca ttcattggtt
    11281 aatctggaag cgcaaaataa tagcctaaaa aaatgatatt tctgatagtt ataaactgaa
    11341 atttcttaat cttgattata acaagctagc atcgctggat gtctcccggc aagaatctct
    11401 gattgagtta agtgcccatc acaatatgat cactgacctt atattacaca atcatcccag
    11461 gatgaaaaca atcactttaa acgacaacca tattgcacat ttaaacgcca aaaacactac
    11521 aaaactggaa tatttaaact taagcaataa caatttactg ccaaccaatg acattgatca
    11581 actaatatca tcaaaacatc tttggcatgt attagttaac ggcatcaaca atgatccact
    11641 tgcacaaatg cagtactgga ctgcagtaag aaatataatt gatgacacta atgaagtgac
    11701 cattgattta tcaggactta atttaaccac tcaaccacca gggctgcaaa acttcacctc
    11761 tatcaatctt gataataacc aactcacaca ttttgatgca accaactacg atagactcgt
    11821 aaagctaagt ctgaatagta atgctcttga gtcaataaat
//
LOCUS       DAJTUP010000108        11689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTUP010000108 DAJTUP010000000
VERSION     DAJTUP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.108"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            118..987
                     /locus_tag="OO468_003238"
     CDS             118..987
                     /locus_tag="OO468_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR6260247.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1190..1543)
                     /locus_tag="OO468_003239"
     CDS             complement(1190..1543)
                     /locus_tag="OO468_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR6260248.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1681..3327)
                     /gene="groL"
                     /locus_tag="OO468_003240"
     CDS             complement(1681..3327)
                     /gene="groL"
                     /locus_tag="OO468_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR6260249.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3371..3664)
                     /locus_tag="OO468_003241"
     CDS             complement(3371..3664)
                     /locus_tag="OO468_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR6260250.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3940..5196
                     /gene="yjeH"
                     /locus_tag="OO468_003242"
     CDS             3940..5196
                     /gene="yjeH"
                     /locus_tag="OO468_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR6260251.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5212..5688)
                     /gene="fxsA"
                     /locus_tag="OO468_003243"
     CDS             complement(5212..5688)
                     /gene="fxsA"
                     /locus_tag="OO468_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR6260252.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6025..7461
                     /gene="aspA"
                     /locus_tag="OO468_003244"
     CDS             6025..7461
                     /gene="aspA"
                     /locus_tag="OO468_003244"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR6260253.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7579..8880
                     /gene="dcuA"
                     /locus_tag="OO468_003245"
     CDS             7579..8880
                     /gene="dcuA"
                     /locus_tag="OO468_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR6260254.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8996..9334
                     /gene="cutA"
                     /locus_tag="OO468_003246"
     CDS             8996..9334
                     /gene="cutA"
                     /locus_tag="OO468_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR6260255.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9310..11007
                     /gene="dsbD"
                     /locus_tag="OO468_003247"
     CDS             9310..11007
                     /gene="dsbD"
                     /locus_tag="OO468_003247"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR6260256.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11044..11619
                     /locus_tag="OO468_003248"
     CDS             11044..11619
                     /locus_tag="OO468_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6260257.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2808 a         3021 c         2875 g         2985 t
ORIGIN      
        1 atatagagtg agcacggcta tttatgttat tttaacaact attactacca agcccattta
       61 tctggcttat aaattaaaat taattattta attgagaaat ggttagggag aacctacatg
      121 gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa tttcattgca
      181 ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat gtctgtcatg
      241 gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca taaactggat
      301 gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa aatggcagaa
      361 aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg tcgggttaac
      421 acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac gctacatgac
      481 ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc gcgagcaatc
      541 attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac ctcactgtta
      601 gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga atatgacacc
      661 tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt gctttaccgc
      721 tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac gcgtgaagca
      781 acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag cccacgactg
      841 aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc gaataacgcc
      901 caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt acaatccacc
      961 agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc tggagccatc
     1021 cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt aaacgcctta
     1081 tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag gcctgataag
     1141 cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat cagttcgggc
     1201 acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac aacattccct
     1261 gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc tggttgcgca
     1321 gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg gaaagccagt
     1381 tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc tgctcgtcca
     1441 caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa cccgccatca
     1501 gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc cccataatca
     1561 atgagctgga cgccactcgc gtcatccgtg tctgaatctt atactagaaa gatgaccaaa
     1621 agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag tgcagggcaa
     1681 ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc ctaagtcagc
     1741 tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca ggccagccac
     1801 agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga tacccatgtc
     1861 gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt cgccgccttt
     1921 aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct gacgcagcgg
     1981 agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt tctgaccacg
     2041 caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag caaccacgcc
     2101 ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt ttttctcttt
     2161 catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg ccagtttcgc
     2221 tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa tctgctgacg
     2281 gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga tgatagtggt
     2341 ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg ttgctttttc
     2401 cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg cgatatcctg
     2461 cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa ctttcacgat
     2521 gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat cttcagcgat
     2581 gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca tttcgcggat
     2641 gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta ctgcgccagt
     2701 ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca taccttcaac
     2761 cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc cttctttacc
     2821 gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt tagcggagat
     2881 ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg ctttcagttc
     2941 ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg ggttcatgcc
     3001 cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg ttgcagtggt
     3061 ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca tctgcgcacc
     3121 catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac catctttggt
     3181 gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg gaccgagggt
     3241 aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac gagcgtcgtt
     3301 accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta tgttcagtgt
     3361 cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg atcaacactt
     3421 cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata acgatgtcgc
     3481 caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca ttgccgacag
     3541 ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg atgccgccag
     3601 cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat ggacgaatat
     3661 tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc ggagcttacg
     3721 tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc aagggggaaa
     3781 aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac ctcacatttc
     3841 atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc gaaaatctac
     3901 gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact caaacaagaa
     3961 ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac tggcgtgttt
     4021 gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc gtggcccgtt
     4081 ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg ccactatccc
     4141 agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct tgagcgagtc
     4201 accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact acaaattgcc
     4261 gccggattcg gccaagcaat gtttggctgg catagctggc aactgttgtt ggcagaactc
     4321 ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag tgctaatcta
     4381 caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg ggcgggcgat
     4441 atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac cgggttattt
     4501 gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc ccatcttgcc
     4561 tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg tctgctgctg
     4621 gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc ctatggtgaa
     4681 caaatggcgg cggcagcatc gcttccaaaa attgtagtac agttgttcgg tgtaggagcg
     4741 ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa catttatata
     4801 cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca ctacctggca
     4861 cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg ctgctgcgtg
     4921 gtgagcacgt tggtaattca tgccttagag atcaatctgg acgctcttat tatttatgcc
     4981 aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa attattgcaa
     5041 ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt actggcaatg
     5101 gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct gtttctgcca
     5161 aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac attaatcctg
     5221 gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat actcaccatc
     5281 aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc gagaaaagcg
     5341 caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata aaagcagaag
     5401 accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa taatcagcga
     5461 aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt gctgcatcag
     5521 cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg acgtgaatat
     5581 aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa agattgaaat
     5641 ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag gtttctcctg
     5701 taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcctt cagcttatat
     5761 tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt aatcccaaag
     5821 cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta aagtgatcca
     5881 gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga tgcaggggat
     5941 aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa gcaaatcatt
     6001 ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga agatctgttg
     6061 ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag agcgattgaa
     6121 aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg cggtatggta
     6181 atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc taaaagtgta
     6241 gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg catggatcag
     6301 ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa caccaacgaa
     6361 gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata tcagtacctg
     6421 aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc gaccggtttc
     6481 cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca actgcgtgaa
     6541 ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg tacccagctg
     6601 caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat cctgctgaaa
     6661 gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa ccttggcgca
     6721 acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc agtgaaaaaa
     6781 ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga agcgacctct
     6841 gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt gaagatgtcc
     6901 aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct gaacgagatc
     6961 aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa cccggttgtt
     7021 ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac tgttaccatg
     7081 gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg ccaggccatg
     7141 ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa atgcattaac
     7201 ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat cggtatcgtt
     7261 acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc
     7321 gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa
     7381 cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc aaaacgctat
     7441 actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc acgtcagatg
     7501 acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc aacttgttaa
     7561 aaaacaagga aggctaatat gctagttgta gagctcatca tagttttgct ggcgatcttc
     7621 ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg ggtgctggtt
     7681 cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc cattatcatg
     7741 gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta tctggttcat
     7801 cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc accgatcgtg
     7861 acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac actgccagtt
     7921 atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc tactgcagtg
     7981 gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt ttacatgtct
     8041 tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt catcccgtcc
     8101 accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa ctccaaactc
     8161 tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact gcgcggtgaa
     8221 aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct gctgggcgta
     8281 gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt tgaaaaaccg
     8341 ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac tctgaccacc
     8401 gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa agcaggtatg
     8461 agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt ttccaacaac
     8521 atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg gctgctggcc
     8581 gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc aaaagcactg
     8641 atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc tttcgctgcg
     8701 gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt acagatggat
     8761 gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt catcccgggt
     8821 actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt catgctgtaa
     8881 tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca tccttccccc
     8941 gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg tgtttatgct
     9001 tgatgaaaaa agttcgaata ccacgtctgt cgtggtgcta tgtaccgcac cggatgaagc
     9061 gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct gcgcgacctt
     9121 gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag aatacgaagt
     9181 gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat gcctgaagtc
     9241 tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg gagacacaga
     9301 ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag cacttccgtt
     9361 tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga tcaagccttt
     9421 gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat caaagacggt
     9481 tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat tgccgacgag
     9541 cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga gatttaccgc
     9601 gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac gttaactgtc
     9661 acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa aaccgttccg
     9721 ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc gcagcaagag
     9781 cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg tattggtatc
     9841 gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt gctgggtggt
     9901 aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt gcaggggatg
     9961 gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca gttccaggcg
    10021 gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt gctggcgatg
    10081 tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg tctcacgttg
    10141 atgagcaatc gccaacaggg cggctcacct agcggtgtgt ttgttatggg ggcgattgcc
    10201 ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct gtatatcgcc
    10261 caaagcggga acatgtggcc gggcggcggc acgctttatc tctatgcgtt gggcatgggc
    10321 ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag cggcccgtgg
    10381 atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt cttcctgctg
    10441 gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg tgtcgcattc
    10501 tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg cgtggtgcaa
    10561 ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg ggcatttggt
    10621 gcgacgcata ccgcgcaaac tcaaacgcat ctcaacttta cacaaatcaa aacggtagat
    10681 gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga tctttatgcc
    10741 gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc gcaggtgcaa
    10801 aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga cgcacaagat
    10861 gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt ttttgacgga
    10921 caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga aaccttcagc
    10981 gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac ggaggaaaat
    11041 agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt acaagggatt
    11101 gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga cgagctacgc
    11161 cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct tagccaacag
    11221 atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga acaaaagctg
    11281 ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc gggctgtcta
    11341 tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca actggccgat
    11401 cagcaaaaaa gcgctgccta cgatttcacc cacgaactgt taaccacgct ggaagttgac
    11461 gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt aagccgaatg
    11521 ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga agatatcctg
    11581 cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag aaaagaagcg
    11641 atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttg
//
LOCUS       DAJTUP010000109        11675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTUP010000109 DAJTUP010000000
VERSION     DAJTUP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.109"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..349
                     /locus_tag="OO468_003249"
     CDS             <1..349
                     /locus_tag="OO468_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6260258.1"
                     /translation="SGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRL
                     QQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQ
                     NDLLLRATGGVCV"
     gene            complement(466..741)
                     /gene="yjfN"
                     /locus_tag="OO468_003250"
     CDS             complement(466..741)
                     /gene="yjfN"
                     /locus_tag="OO468_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR6260259.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(890..1219)
                     /gene="bsmA"
                     /locus_tag="OO468_003251"
     CDS             complement(890..1219)
                     /gene="bsmA"
                     /locus_tag="OO468_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR6260260.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1401..2150
                     /gene="yjfP"
                     /locus_tag="OO468_003252"
     CDS             1401..2150
                     /gene="yjfP"
                     /locus_tag="OO468_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR6260261.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2147..2902)
                     /gene="ulaR"
                     /locus_tag="OO468_003253"
     CDS             complement(2147..2902)
                     /gene="ulaR"
                     /locus_tag="OO468_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR6260262.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3010..4074)
                     /gene="ulaG"
                     /locus_tag="OO468_003254"
     CDS             complement(3010..4074)
                     /gene="ulaG"
                     /locus_tag="OO468_003254"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR6260263.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4429..5826
                     /gene="ulaA"
                     /locus_tag="OO468_003255"
     CDS             4429..5826
                     /gene="ulaA"
                     /locus_tag="OO468_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR6260264.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACVSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5842..6147
                     /gene="ulaB"
                     /locus_tag="OO468_003256"
     CDS             5842..6147
                     /gene="ulaB"
                     /locus_tag="OO468_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR6260265.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6157..6621
                     /gene="ulaC"
                     /locus_tag="OO468_003257"
     CDS             6157..6621
                     /gene="ulaC"
                     /locus_tag="OO468_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR6260266.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6635..7285
                     /gene="ulaD"
                     /locus_tag="OO468_003258"
     CDS             6635..7285
                     /gene="ulaD"
                     /locus_tag="OO468_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR6260267.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7295..8149
                     /gene="ulaE"
                     /locus_tag="OO468_003259"
     CDS             7295..8149
                     /gene="ulaE"
                     /locus_tag="OO468_003259"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR6260268.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8149..8835
                     /gene="ulaF"
                     /locus_tag="OO468_003260"
     CDS             8149..8835
                     /gene="ulaF"
                     /locus_tag="OO468_003260"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR6260269.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8964..9239)
                     /locus_tag="OO468_003261"
     CDS             complement(8964..9239)
                     /locus_tag="OO468_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6260270.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9566..9961
                     /gene="rpsF"
                     /locus_tag="OO468_003262"
     CDS             9566..9961
                     /gene="rpsF"
                     /locus_tag="OO468_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR6260271.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9968..10282
                     /gene="priB"
                     /locus_tag="OO468_003263"
     CDS             9968..10282
                     /gene="priB"
                     /locus_tag="OO468_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR6260272.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10287..10514
                     /gene="rpsR"
                     /locus_tag="OO468_003264"
     CDS             10287..10514
                     /gene="rpsR"
                     /locus_tag="OO468_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR6260273.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10556..11005
                     /gene="rplI"
                     /locus_tag="OO468_003265"
     CDS             10556..11005
                     /gene="rplI"
                     /locus_tag="OO468_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR6260274.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11076..11639)
                     /locus_tag="OO468_003266"
                     /pseudo
     CDS             complement(11076..11639)
                     /locus_tag="OO468_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2764 a         2871 c         3083 g         2957 t
ORIGIN      
        1 ttccggcaat attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata
       61 cgacttattg tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc
      121 ggttcgtcgt ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac
      181 tcatcagcta ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat
      241 ggcgcaggcg tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca
      301 gatccagaat gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac
      361 tgatgcgacg ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag
      421 gcgctcgcgc cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat
      481 cgcctgtacg cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca
      541 tgacgcacct tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc
      601 tgaaatattg ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact
      661 ggcgaattct gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg
      721 tgaggcaagt aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc
      781 attccatcgc ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa
      841 cgcataggga cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt
      901 acaaaatggc ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa
      961 cgtaataatc agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg
     1021 gagaaccccg aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc
     1081 ggcgaatctc ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg
     1141 cgctacaggc acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat
     1201 tacgtttcct gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc
     1261 gaattgttac ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct
     1321 caacattatg aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac
     1381 cagacaaaaa ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg
     1441 ttcttcatgc ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc
     1501 acggctttac ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg
     1561 gtttgcgggt gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag
     1621 cgcggcggtt aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt
     1681 tacgtgcggc aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg
     1741 cgtcgatggg cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta
     1801 ccgccagcat gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc
     1861 tgatacctga aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag
     1921 agtgggaagc gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg
     1981 gcctcgacga tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg
     2041 agacgggacg ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgcatta
     2101 cgcctgaggc gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa
     2161 tgctgacccc ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga
     2221 tcagcatatc gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac
     2281 tgctatcaac cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg
     2341 ctgtcagcat atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca
     2401 tccagtgccc ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac
     2461 ttttgttgta ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat
     2521 ttgccagcgg tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa
     2581 gcagaaacgc ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg
     2641 aggccgcttt agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg
     2701 gcgtccagcg cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt
     2761 tgccgctttc gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac
     2821 gctcaacgac tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt
     2881 gatgtctttg tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat
     2941 ttgaaatagc caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata
     3001 aacgacagat tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc
     3061 gaaaccgcgc ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc
     3121 gccaacctgc cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca
     3181 cagcacgcgg atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg
     3241 gataaccact ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt
     3301 gtcggtaata ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc
     3361 gttaccgtgc ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc
     3421 gccaggggtt ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc
     3481 agccgctttt tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc
     3541 atgaatttca atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc
     3601 tttcggtacg ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac
     3661 gtcatcagca cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg
     3721 atcgtgagtc gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg
     3781 ggtggtgcgt aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc
     3841 ctgtttcatt aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca
     3901 aacgttggtg ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc
     3961 aaatgtgcca ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc
     4021 cgggaaagtg ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat
     4081 ttaccttcat gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac
     4141 tattccttca ggaaggtaat gaccattttt tgactttttg ccagggaagt tgttgttgat
     4201 tttgagtggg gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa
     4261 ataattgttt cgttgtgtga atcccactct atccatgtgg aattatttgc gggtcgcgtc
     4321 acatttaatc ataaataatc ttgttgtgat tacttttgaa aattagagtg agtgcacaac
     4381 attccgggtg tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc
     4441 tacaacatct ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt
     4501 attgtgacct gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc
     4561 acgattaaaa ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc
     4621 accttcaaac cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc
     4681 gatacttacg cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta
     4741 ggttacgcgg tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt
     4801 accggtattc gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt
     4861 gccgttacgt tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt
     4921 ctggtttcgc tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg
     4981 acggacggct gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat
     5041 aaagttgcgc cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc
     5101 tggctgaata ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt
     5161 ggtgccattc tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac
     5221 tggacggtct atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact
     5281 cagggtgtgc gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc
     5341 ctgatccctg gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac
     5401 gccgtggtct ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg
     5461 gtcgcctgcg tttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac
     5521 gccaccatcg gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt
     5581 ctggtgatgg ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg
     5641 agtgcctgga tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc
     5701 ttctccatcg gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc
     5761 ttcgctggcc gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct
     5821 gcttaataag gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa
     5881 ggcagttcca tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac
     5941 cacacggtaa atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc
     6001 atcatcgctt ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt
     6061 ggcgtgcgca acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa
     6121 gcgcatttcc cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg
     6181 gagaataaat ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc
     6241 ggtgttgacc tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg
     6301 gatgcggttg aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac
     6361 ggtcgcccgg aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg
     6421 ctggagttca accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc
     6481 gatgccaaca cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa
     6541 gaaaatttcg accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac
     6601 cgcaccaacg cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc
     6661 gctggacaac cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt
     6721 cgacattatc gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga
     6781 tctgaaagcg ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg
     6841 caaaattctt tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg
     6901 tgcggatatc aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt
     6961 gcagatcgaa ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg
     7021 tattcagcag gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg
     7081 tgaagccgat atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac
     7141 cggtggcctg gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat
     7201 cgccggtcgc agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg
     7261 ttccatcgcc gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg
     7321 catctatgaa aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa
     7381 aacgttgggc ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg
     7441 ccttgactgg agccgcgaac agcgtctggc gctggtgaat gctattgttg aaaccggcgt
     7501 gcgtgtgccg tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga
     7561 cgcggtgcgg gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt
     7621 tggcattcgc gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga
     7681 aacgcgtcgt cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca
     7741 ggtgacgctg gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct
     7801 gggttacgcg cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct
     7861 gtcggcgtgg gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc
     7921 ggtgcatgtg aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt
     7981 ggtggatttc gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct
     8041 gattgagatg tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgtgtga
     8101 ttgggtgaaa gcgcgcatgg ccaaagcagg catggtggag gcggcataat gcaaaagcta
     8161 aaacagcagg tgtttgaagc caacatggat ctgccgcgct atgggctggt gacctttact
     8221 tggggcaacg tcagcgctat cgaccgcgaa cgcgggctgg tggtgatcaa gcccagcggc
     8281 gttgcctacg aaaccatgaa agcagacgat atggtggtgg tcgatatgag cggcaaggtg
     8341 gtggaagggg agtatcgccc atcttccgac actgcgacgc atctcgaact ctaccgtcgt
     8401 tacccgtcgc ttggcggcat tgtccatacc cactccactc atgccaccgc atgggcgcag
     8461 gcggggctgg cgatcccggc attaggcacc acgcacgctg actacttctt tggtgatatc
     8521 ccgtgtacgc gtggattaag cgaagaagag gtgcagggtg agtatgaact gaacactggc
     8581 aaagtgatta tcgaaacgct gggcaacgcc gagccgctgc atacgccggg aattgtggtg
     8641 tatcagcacg gaccgttcgc ctgggggaaa gatgcccacg acgcggtgca taacgcggta
     8701 gtgatggaag aagtggcgaa aatggcgtgg attgcgcgca gtattaatcc acaactcaat
     8761 catatcgaca gctacctgat gaataaacac ttcatgcgta agcacggtcc taacgcttat
     8821 tacgggcaga agtaaaacac gcgctgcgga aatttccttc ctcgggagat aactggtcta
     8881 attccgcagc cgtttttcaa aaaaagcccc ctgcgaaggg ggcaaagcaa actatggcaa
     8941 tgtttcgttg gttatacctg gtgctagcga taaatatccg cgctggcgtg catattgccg
     9001 ttactccccg gttcccgcat cagaattacg tggtagtacg ttgcgccttg cgcatctgtt
     9061 tcttcattta atgcctgacg tgcttcgctt tcagtggcga aattatgatt gatataaatc
     9121 acgcctaagc tttgcacatc gtccatattt ctggcctggt ggttattaat ttcaatggct
     9181 gcccatgtat ttgcacttag caaaagcaca gccagaaggg ctaaaactcg actgaacata
     9241 gatacctcct cgacggctga ctttgtgtgc tctcctctgt gatgatcttc tgatttaatt
     9301 ttaatcaatg ataaagaagt tgatggtgac catttctgat gcagttgttc aaaaaaacac
     9361 tgtgacggaa tgtgatgaac ttcaaatcag cggtttagag gttatttgcg aaaggggaga
     9421 tttatttctg ctccgccctt gagtttagcg gggtatgcca gtactataac gcgtcatttt
     9481 tcagccgacc tttaacacgt tccttgcctc cccgggattc ggctgaccca gacaggaggc
     9541 tgaataatcc gtaaggagca attcgatgcg tcattacgaa atcgttttta tggtccatcc
     9601 tgaccagagc gaacaggttc cgggcatgat cgagcgctac actgctgcca tcactggtgc
     9661 agaaggcaag atccaccgtc tggaagactg gggccgccgt cagctggctt acccgatcaa
     9721 caaactgcac aaagcacact acgttctgat gaacgttgaa gctccgcagg aagtgatcga
     9781 tgagctggaa actaccttcc gcttcaacga tgccgttatc cgcagcatgg ttatgcgtac
     9841 caagcacgct gttaccgaag catctccgat ggttaaagcg aaagacgagc gccgtgagcg
     9901 tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa gctggggatt ctgaagagta
     9961 atttctgatg accaaccgtc tggtgttgtc cggcaccgtg tgcaggactc cccttcgaaa
    10021 ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt gagcatcgtt ctgtgcagga
    10081 ggaagccggc tttcaccggc aggcatggtg tcaaatgccc gttattgtta gcggacacga
    10141 aaaccaggcc attactcaca gtataacggt cggcagtcgc ataaccgttc aggggttcat
    10201 ttcatgccac aaggcaaaga acggactgag caaaatggtt ttgcatgccg agcagattga
    10261 attgatagat tctggagact agccatatgg cacgttattt ccgtcgtcgc aagttctgcc
    10321 gtttcaccgc ggaaggcgtt caagagatcg actataaaga tatcgctacg ctgaaaaact
    10381 acatcaccga aagcggtaag attgtcccaa gccgtatcac cggtacccgt gcaaaatacc
    10441 agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct gtccctgctg ccgtacactg
    10501 atcgccatca gtaatcggtc acggtccatt aatacgactt tgagaggata aggtaatgca
    10561 agttattctg cttgataaag tagcaaacct gggtagcctg ggtgatcagg taaacgttaa
    10621 agcgggctat gctcgtaact tcctggtacc gcagggtaaa gctgttccag ctaccaagaa
    10681 aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa gctaaactgg ctgaagttct
    10741 ggcagctgct aatgctcgcg ctgagaaaat caatgcactg gaaactgtta ccatcgcgtc
    10801 taaagctggc gacgaaggta aactgttcgg ttccatcggt actcgcgaca tcgctgacgc
    10861 tgtaactgca gctggcgttg aagtggctaa gagcgaagtt cgtctgccga acggcgttct
    10921 gcgtaccact ggcgaacacg aagtgagctt ccaggttcac agcgaagtat tcgcgaaagt
    10981 gatcgtaaac gtagtagctg aataattcgt tattcaacga gacgtaaaaa cgccgaccat
    11041 tggtcggcgt tttgctttct atttttcgtc aggcattaat ttcgcaagta gatccattca
    11101 ccattctctt gtgtccataa gtcaggatgg tttgctggcg ttagatgcat acgatcactc
    11161 cttatgtctc ggtcctgcgg cactcgctcg aaacctaaac ccgagtagat tgcggctgaa
    11221 tttgataagg aacgtagacc aattttcaaa tcatggggta aatcagaata cgcgtttagc
    11281 aatgcagcaa tgatgaatcc gagggcatta gaaacgcctg taacagtgtg aagttggtcg
    11341 atataaagat cgattgttgg tagttcagat gagcagcaat taggcatttg ggcaggaagg
    11401 gttacaacgt atcctatcgt ttggccggta tagtctggag tactataacc aggtcccaaa
    11461 acaacctcgt gtattgtgta tttactgttg ggttcgaagg attcgacatc ttgcaataaa
    11521 tatttcagtt ctgcggttaa cctcgcaccc gcgtaagctt tattgtattt ctgggcgtga
    11581 atattatcta atgtagcttc atggttccat ccaaaagaat tatcaccatt cattgttact
    11641 gaatcgccac gggtttaaca gacacctcag agtca
//
LOCUS       DAJTUP010000110        11610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTUP010000110 DAJTUP010000000
VERSION     DAJTUP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.110"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..2290)
                     /locus_tag="OO468_003267"
     CDS             complement(<1..2290)
                     /locus_tag="OO468_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR6260275.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIR"
     gene            complement(2224..2847)
                     /locus_tag="OO468_003268"
     CDS             complement(2224..2847)
                     /locus_tag="OO468_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR6260276.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2844..4532)
                     /locus_tag="OO468_003269"
                     /pseudo
     CDS             complement(2844..4532)
                     /locus_tag="OO468_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4681..7308)
                     /gene="gyrA"
                     /locus_tag="OO468_003270"
     CDS             complement(4681..7308)
                     /gene="gyrA"
                     /locus_tag="OO468_003270"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR6260277.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7455..8177
                     /locus_tag="OO468_003271"
     CDS             7455..8177
                     /locus_tag="OO468_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR6260278.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8305..11610)
                     /gene="yfaL"
                     /locus_tag="OO468_003272"
                     /pseudo
     CDS             complement(8305..11610)
                     /gene="yfaL"
                     /locus_tag="OO468_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2676 a         3229 c         2881 g         2824 t
ORIGIN      
        1 cccggatatc aagctgagga acggcaactt tgatcccggc gttctgaaaa ctgtactgac
       61 cattacgggt atacagcacc gaaaaagtga tgttaggcgc aaaggaattg ctcactggta
      121 cccgggcttc atacagggta tcgtttaaac gttgtagcgt tagccagttt gccggatgcg
      181 aaagcagcga ctgctgttcc acgcgatcgc gttccagcgt caataacgct tcatcaattg
      241 gctccgggaa agtaatcagc atcttcgcgg tttcgcctgg ctggtacagc gttttatccg
      301 ccacgatatc taccgtacca gtatgcgccg tgctgccctt accgctgacg gcatgactta
      361 acccagcgag aattaagccg tctttatcgc gtaatgtcag attgtagttg ccaggtttag
      421 cgaaattgac ggtaaaggat ttgccgcctg acggtagctc tccgctatgg ctcgtgcggt
      481 cttcgagacg caaccattca tacgtaacag gaacctgttt tgaagattcc agcgcggcat
      541 aacggaacac aaccgactcg ccgctattac tgtattgtgc ggcggtactt aaagagtaat
      601 gcgccaggcc gcgttcaatg aggatctctt tggtggtggt gacgcgatac gccgcgccgt
      661 cactggcgga gacggttaac aaatagcggc tcggtttatc ggcggcgggg agatttaacg
      721 ccacatgacc gctggcgtcg gacaccgttt cgctgccttc cagcgacacg gggaaacgtc
      781 cggcataacg caaatcgtta ccgaccattg ataattgctg agcgcgcaaa cttaactgca
      841 cgcgggcatt ttttaccggc tcgccatccg ggtagagcag ttgcagtttg ccgctgaccg
      901 cttcgccagt tttgaactct tttttgtcga gagctaaacc aatctcgaaa tgtggcttga
      961 tgtagtttgc cacgcgaaaa ctgctgctat agacctgatt gcggtaagca agacgtaact
     1021 cataacctcc ggcaacggca ttttctggca ggcggaaact tccctgcccg ccattgcgcg
     1081 catccagcgt gacatcgacg gtttgcaaca gactgccgtt agcgtccagc accgaaagct
     1141 tcgccggggc gctgacgatg ggggatgaat gcaacggatc gcggaactcg cggcccatca
     1201 ctttaacatc gacacgatcg cctgcgcgat atagcgggcg atcggtaaaa atatacaagc
     1261 gggtgttgta gatttcgctt tcgtagaaga agttctcgga gacaaaaacg ccgccttcag
     1321 catccttacc cagaatgtat gaacgttctg gcgatatatg ttgtaactgc aaagtaccgc
     1381 tgtcatcggt caccccgcgg gtcatcacgc caagaccttc agtccacaag atctctgagc
     1441 cgggcttcgc ttcaccctgt tttttacccg cggtccatac cagaagctcg ttacctgaca
     1501 ctttgctaag cgccacggta tcggaaacaa acaccaccgt cgtcgcccga tacccaccta
     1561 ccatcgcctc gacgaggtac agccccggct cttgttggcc gagaggaata taaatattac
     1621 ccggctgcgg cgagatgaaa ttgctggatg cgccttccag ttttacccct tgctgcggct
     1681 cgaccggttt agcctgccat agtggataac ggaactgttc caccaaggga tattttttca
     1741 gcggggaaaa ctggttgttc tgtacataac gggaaggttt aataatggca ttgccgagat
     1801 gtaattcggg taatgcctga gtcacattct gccgtgactg agaagagaaa gtacgctgca
     1861 tcacgcggcg agatttgccg taccagttat cccacagcca ggtcagcgta ttgttcagcc
     1921 cgtcgcccag atattgcggt tgcaccacaa tgcgatgcag gtttttctgc tggcgcaaaa
     1981 atgccatcgg gtcaggaata cgatataggc gaacgtccac gccgccgtac tcttccatct
     2041 gatagcgccg ataatcacgc cccggcgctt ccagtcgcac tttcgcctct tcactgctgc
     2101 taaaactgct gtcagcaagc aaaaagaatg ttcccccggc gggcggcgca tagttactgg
     2161 aaggaagcga atcatcagct ttagcaagcc ctgttcccac aagagacagg gacaagcacg
     2221 ctgctatcgc gccagaaaat ttaaacgata aatgccaatg aaattgggat tggaatcgtt
     2281 gggtatccat cgggtgtcct tccatgtcat aagttgttgc agactgactg cgcgcattcc
     2341 gttgtcagtt ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca tccagaccat
     2401 taagtgctgg gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg cctggtttat
     2461 gtcttggcca ataaactggc tgttgtactg aatcaaatta atcgcggtca cgtaggggcc
     2521 gtttttcccg ctcccctgat tccagttttg cgccagttga cgctgttcag gtgttagcgt
     2581 catctctggc ggcaaatact ggctagataa accgttactt tttagccatt tactgtcgtg
     2641 aactttcagc gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct gctgatacca
     2701 gcgtggactt ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc aggcgcgaaa
     2761 cagcctggat tgttcgacat tcagcatttc gctatgggca acaacacaac acagccagca
     2821 aatcagcgcc agcagcccgt gtctcatagc ggctgccagg ttatgggtag ccactgccag
     2881 gcgacaccgg gttccatctg ggccagcttc atgacataac gcggttgttg agataaagcg
     2941 tccagcttcg gcattaataa agtttgtgcg gcgttataaa aaaccggttc gagattcttc
     3001 ggcagactgg tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg attgatatag
     3061 agcggaacga tgccatcagt gggtattaca tccaccattg ccgggcgggt tttattcagt
     3121 gtttgcaggg cgttattaac cagcgtgtca tcgagggaga aaagcagcgt tttgttttgc
     3181 atcgccagcg acacacggaa aaaataatcc gacattaatt gatcgggttg cgccgcctgc
     3241 gctttcggat actgaccgta tcgggaactc acttcgcgac accagatttg cgcttcgccc
     3301 tgctgagtct ggcttaccgg caaaacacct tctggcgctt tgctttcgtg cgcaccaata
     3361 ttttgcgtaa acagtttccc tggcaattgc gcctgttcgg cagtgccatc aaactgaccg
     3421 acaaacagcg gggtttgcaa ttttgagtct tcataccagc acagccccgc ggcaccgtct
     3481 aacgccccat tcagcttgtc gttttcctgg ctgatgtgcg aaagcatctc ttcggcaata
     3541 ccgtgtgaat acggcaccgc cacacagaag ctggctccgg caggcatact gttccagacc
     3601 ggcgtaaaat cgaaactggc atctacgctg gcggattcat catttaacgc cacaaaactg
     3661 tgccagccgt cgttacccat ttcgaagcgt acgccagcaa aagaaggcat taaccgctgg
     3721 tagccaaaac ccagcaacct ggcgctgacc acgatgcgca ggcgtactgg cgttttttcg
     3781 gctgtacgct cttccaggcc aaagctggct tgccagcgtt ttttaccgct caacaaatca
     3841 cttgcgatcg ccgtggcttc ggtatcctgc tgatcatctt taaacaacat atccgtactg
     3901 gaaaacacca gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt gccgttatag
     3961 cgcaactgat aaacaggaat agtttcacta tttatcttga tgctactgat ttccgttttg
     4021 cttaactagc tgtcgctggt agcggcaaac aacaatggct ccagcagttt gcttaaacca
     4081 ctgcgctgga tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg ccatagcgcc
     4141 gcctgcgcgg gctgatctaa gagtgacgaa aagagcttat ctttcagcgt gagatcgtgt
     4201 tcatagacaa tgcggcgaat gctgccttca atgcccagac gatcggcatg attctgataa
     4261 tagaaaacga aatcttcgct cagaacatcg tggagaaacg gaatggtgag gagatctttg
     4321 ggaagctggc tcagagagtc gctgtctaga aagaggtccg gctcattgag atcgatttgc
     4381 agattgttgt gcaccaccag cggcgacaac gttttttctg gcccactgcc agcatattgc
     4441 aacgcccaga cgccagcgga aagcagtgct attgcgccaa aacctacaag accatagaac
     4501 cgccagcctt tcgccttttt ttcaccactc attgccacat tccttgtgta tagccagcca
     4561 ttttttacgg gcacagccaa actttaccgc gccctaatac gacaaaagcc cagactttgc
     4621 agcctggact tttcaattca aacaagggag atagctccct tttggcatga agaagtaaaa
     4681 ttattcttct tctggctcgt cgtcaacgtc cacttccgga gcaatttcat catccccttc
     4741 cgcggcactg ccgtcgatgg tatccagatc ttcctcgtca accggttcag caacacgctg
     4801 cagacccact acgttttcat cttccgcagt acggatgagg atcacgccct gggtgttacg
     4861 gcccacgatg ctgatttctg aaacgcgagt acgtaccagc gtaccggcat cggtgatcat
     4921 catgatctgg tcgcagtcat ctacctgcac cgcgccaacc actaaaccgt tacgttcggt
     4981 aaccttgatg gagataaccc ctttcgtcgc acgcgacttg gttgggtatt ccgccactgc
     5041 agtacgttta ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc cacgaggcac
     5101 gatcagagag acgactttat cgccttcacc taagcgaata ccgcgcacac cagtggtatt
     5161 gcagcccatc gcacggacag aagactcttt aaagcgcacc actttacctt cggcggagaa
     5221 cagcattact tcatcttcgc cactggtcag atcaacgccg atcagctcat cgccgtcaac
     5281 cagtttgatc gccactttac cggcggtacg cagacggttg aattcggtga ggacggtttt
     5341 cttcacagta ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa actcggtcac
     5401 cggcaggatc gcggtgatac gttcgtcctg ttccagcggt agcaggttga cgatcggacg
     5461 accgcgcgcg ccacgagtgg cttccggcaa ctggtaaact ttcatcgaat agacgcgacc
     5521 acggctggag aagcacagaa tatggtcgtg cgtgttcgcc accagcagtc ggtcgataaa
     5581 gtcttcttct ttaatacgtg cggcagattt acctttcccg ccacgacgct gcgcttcgta
     5641 ttcagaaagc ggctgatatt taacgtagcc ctggtgagag agcgtcacga ccacatcttc
     5701 ctgggtgatc agatcttcca ggttgatgtc tgcgctgttg gcggtgattt cagtacgacg
     5761 tttgtcaccg aactgttcac gaaccagctc cagctcttcg cggatcactt ccatcagacg
     5821 atcggcgcta ccaagaatac gcaacagttc cgcgatctga tccagcagct ctttgtattc
     5881 gtcgagcagt ttttcgtgct caaggccggt cagtttctgc aaacgcagat ccagaatcgc
     5941 ctgagcttgc tgttcggtca ggtagtacag accatcacgc acgccgaact ctggctccag
     6001 ccattccgga cgcgcagcat cgtcgccagc acgttccaac atggcggcaa cgttgcccag
     6061 ctgccacgga ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg catgacggat
     6121 cagttcgatg atcgggtcaa tgttcgccag cgccacggct aatgcttcaa ggatatgagc
     6181 acgatcgcga gctttacgca gttcgaaaat agtacgacgg gtcaccactt cacggcggtg
     6241 acgaacaaac gccgcgatga tgtctttcag gttcatgatc ttcggctgac catggtgcaa
     6301 tgccaccatg ttgataccga aagaaacctg caactgggtc tgggagtaga ggttgttgag
     6361 cacaacttca ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac cgtctttgtc
     6421 agactcgtca cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca gttccgcaat
     6481 cttctcgatc aggcgcgctt tgtttacctg atacggaatt tcgtggacga taatggtttc
     6541 acgtccggtt ttggcgtcaa cttccacttc agcacgggcg cggatatata ccttgccgcg
     6601 accggtacgg taagcttctt caataccgcg acgaccgtta atgattgccg ccgtcgggaa
     6661 gtccggcccc gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat catcgatata
     6721 cgccagacaa ccgttgatga cttccgtcag gttgtgcggc gggatgttgg ttgccatacc
     6781 tacggcgata ccggaagaac cgttcaccag caggttagga attttggttg gcatgacgtc
     6841 gggaattttt tccgtgccgt catagttatc aacgaaatcg accgtctctt tttcgagatc
     6901 ggccatcagt tcatgggcaa ttttcgccag acggatttcc gtataacgca ttgccgccgc
     6961 agagtcgccg tcgatggaac cgaagttacc ctgaccgtct accagcatgt aacgcagcga
     7021 gaatggctgc gccatacgga cgatcgtgtc ataaaccgcc aagtcaccat ggggatggta
     7081 tttaccgatt acgtcaccaa cgacacgggc agatttttta taggctttgt tccagtcatt
     7141 gcctagtacg ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc catctcggac
     7201 atctggcagc gcacggccaa caatgaccga catcgcataa tccagatagg agctctttag
     7261 ctcttcctca atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct aaccgctatc
     7321 cctctactgt atcccggatt caaaggtcgc aaattataac acagccgcgc agtttgaggt
     7381 aaacctatac gctttattca catccaatgc ctgatatact cgtttgtctt gccaattacg
     7441 gagtagaagt gccaatgaat gccgaaaaat cgccggaaaa ccataacgta gaccacgaag
     7501 agatcgctaa atttgaagcc gtcgcctccc gctggtggga tcttgaaggt gagttcaaac
     7561 cgttgcaccg cattaacccg ctgcgtctgg gctatattgc cgagcgtgct ggcggtttat
     7621 ttggcaaaaa ggtgctcgat gtcggttgtg gcggcggcat tctggccgag agtatggcgc
     7681 gcgaaggcgc gacggtgacc ggtctggata tgggctttga gccattgcag gtggcaaagc
     7741 tgcacgcgct ggaaagcggc attcaggtgg attacgtgca ggaaaccgtg gaagagcacg
     7801 cggcaaaaca tgccgggcag tatgatgtgg tgacctgcat ggagatgctg gagcacgtac
     7861 ccgatccgca gtcagtggtc agagcctgtg cgcaactggt gaaaccaggc ggcgatgtct
     7921 ttttctcgac gcttaaccgc aacggcaagt catggctgat ggcggtggtt ggcgcggaat
     7981 atattttgcg catggtgccc aaaggtacgc atgatgtgaa gaagtttatt aaaccggcag
     8041 agctgcttgg ctgggtggat cagaccagtt taaaagagcg acatataacc gggctacatt
     8101 acaacccgat cactaattcc tttaagctcg gccccggcgt ggatgtgaac tatatgctgc
     8161 acacgcagaa taagtgaggt tgatgtttgg ccgcgccaat gcctgatgcg acgcttgcag
     8221 cgtcttatca ggcctacaaa tgctctccgt aggccagata aggcgtttac gccgcatccg
     8281 gcaattgtga tggtcagaca gcgattacca tttcaccgtc atcgacaaaa accctgccgt
     8341 ctgggcaaaa tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg acactcgctg
     8401 actgatattg cccgtgactc ccacttttat ttcaccccgt agcttcaccg catcgtcact
     8461 gatagtgctg ccatcttctt caatttccgt cgcatgggga tcgtgatagt aattcagatc
     8521 taatgttggt gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca gccgcgtctg
     8581 aatatcatcc ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa aatcatcctg
     8641 ctgcacgccc tgataaatca cctgcgcctg cggttcaatc accacaccac gccccggtaa
     8701 ccactgatac ccagcttcca gcgaggcgat aatccccgac gaatggtaac gatccacgcc
     8761 atcttcatgt tcggaaacat cattgttaaa ccacgcgtat tgcagccagg ccccctgctt
     8821 ctgattaccg tgctgatacc agcttgaggt cagcccaacg gcataaccgt ggttctggtt
     8881 atcggcgcga gttccggtca tattcgagcg gctgtcgccc tgattatcac tgtagccacc
     8941 aaccgcccca agcatccact cgccatccgt gccccagcgc ccgctaaaca agccgccgct
     9001 aagctgcacc gtagaagtgt cttcctgctg agccagttgc cctgccgccg tgtaatgata
     9061 acgtccgccg ataacacgta aattcagcgt ctgaccatcg ccaccagcgt gatcgcgtcg
     9121 ctccatcata aacgcctgat ttgccgcccg caggttattg agataaccgc caactttggc
     9181 attcagcacc ggctggtaag caggcgtagg ggccgggtcg ggggttgggt caggatccgg
     9241 cgtgggatca ggatcgggag tcgggtctgg ctcaggtggc gatggcggcg ttacttcttg
     9301 cgatcgcaga taccagtcgt tgttatcttc cactagcgtg tagtcatacg ctcccatgtt
     9361 gacgtagcca ctgccagcca gactgaactg tgcattgttt tgaaactgcg tgggatcagc
     9421 tgcgaaatca accactttaa tgcctgtcga tgtcggctca ccaatccctg taatggagtt
     9481 aaccaccaca gttgtgttgc cagcagtatt accgttcatc accaattgat cgcttaccga
     9541 gtcatcgccg tttaattcgc tgtcgaatag tagcgtaccg ccgccggtat aatcaccgtt
     9601 taccgtcagc gtatcgccag cgacgccatt ttgcaggcta accgcaccgg acgtattggt
     9661 cagatcacca ttaaccgtca cgtcagcaag ataggtcagg ctgtcagttt caaggttggt
     9721 attaagctcg cctgtcaacg tcacgccatc ggtagcattt accagcgtcc catcgccgtt
     9781 aaatagtgag gcattaaggg cgacggaagt atcctgcccg gtcagacgca aaaccgtacc
     9841 atcgctgatg tcgatagtgc cgctggaagt agcatcaatt gactgaatat cctgatccgc
     9901 gccagtaacg aacgttgccc catcaccaac ccacagtgac gaagcataag gaaagatatc
     9961 cgcaacatcg cctttcaggg tgccttcttc gacacgtacc gccgactgcg ttgtaccgct
    10021 ggcggtcagc tccagcgtac ccgcccccgt tttagtaaat gtgctaccct catcctgatg
    10081 ctgcccgctg ctgtcagcca tcagtgcgcc ccactgcgtg tctacccccg catcaactgc
    10141 cacttcaccg tcggcgcgca tttcaatatc acgcccatgc tcagtcgtcg tagagcgagt
    10201 atcgacattg gcagtaatct ccatcacgct ttcttgttac ttatcggtaa agataacctg
    10261 gcggttatag tgggtatctc caagctgcga gttgtcgctc accatcagcg taccggaggc
    10321 gatttgtgtt gtgcccaaat aactattatt gtttgccaga cttacctgcc cgtcgctcgc
    10381 atcgaccacc acgtagacgc catctttccc gtcgccgccg atatgcaaat tgtcgccacc
    10441 tgcaagatct accacatcct gactgccgat aaccgttccg ctgctgccac tgacttcaag
    10501 gccatcattg tatgatgtgc cgctctgcca agtggagaaa tcagagagat cgagcacacc
    10561 gccgtttaac acgatcgact gcggatcgtc ctggagagcg gtaagatctg ccgcgtcgcc
    10621 ttccagcgaa agcaccgcac catcatcaac gactatatcg ccggttagcg ccatcgactg
    10681 cgcccctgcc agcacgtagc tgccgttttg cgcaatggtg agctgaccag caccttcgat
    10741 gatgccagca aaactgccct gattaacagt aacgttgcca ccagcatcga tatttaaagt
    10801 gccattctga aagcccgtca atgcgtgcac aaaagtttgt tgggtcgagc caacgtttag
    10861 ctcagcctga ttctgatact gatcaatact ccctatcgtc agaccgtagc agtcttgcgg
    10921 atcgtcctgg caatgcgtat cgccgacatt catcagggag ttgctgcgac ccagggtaac
    10981 ttcaccgttt tcaatctgca tctcaccagt aaagtcattg ttatctgcat taagtaccag
    11041 atcgccggaa cctgttttgg tgattaaccc ggtaccagca atagagtcaa cagctccgtc
    11101 attctctgta ttgccgataa ccagcgtttt tccgtcggca atatcaaagg taacttcact
    11161 taagccgaga tacataaagc cacccgccgc agtggaggga ccatctccat agcctgctgc
    11221 gctattgttc tcatcgacta acacgccgcc gttctggctg tagctgtcat caacagaaat
    11281 atcaataaga tagggagccg tcgcgctatt ggtatatatc gccccgccat aaccttcggc
    11341 tgtgttattt gtaaaggcgg tgttatttat tatcgtataa cctgaaggat gcttgctgtc
    11401 gctattatta tcggtaacat cgattgcccc gccatcgcca tcactgtaac ttgttgatgt
    11461 atatgcctgg ttattgttaa aaacatcatc acttaaatag acgtcattat taatggtata
    11521 aattgcgcca cctttgccat cattagcgat attattgatg gcgttagtga cacgtaaatc
    11581 gacggcacca gtatcattag taccagaaga
//
LOCUS       DAJTUP010000111        11589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTUP010000111 DAJTUP010000000
VERSION     DAJTUP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.111"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(177..869)
                     /gene="fimC"
                     /locus_tag="OO468_003273"
     CDS             complement(177..869)
                     /gene="fimC"
                     /locus_tag="OO468_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6260279.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1089..1631)
                     /gene="fimA"
                     /locus_tag="OO468_003274"
     CDS             complement(1089..1631)
                     /gene="fimA"
                     /locus_tag="OO468_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6260280.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2112..2978
                     /gene="folD"
                     /locus_tag="OO468_003275"
     CDS             2112..2978
                     /gene="folD"
                     /locus_tag="OO468_003275"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR6260281.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            2980..3192
                     /gene="ybcJ"
                     /locus_tag="OO468_003276"
     CDS             2980..3192
                     /gene="ybcJ"
                     /locus_tag="OO468_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR6260282.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3300..3809
                     /locus_tag="OO468_003277"
     CDS             3300..3809
                     /locus_tag="OO468_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6260283.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVLAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(3845..5230)
                     /gene="cysS"
                     /locus_tag="OO468_003278"
     CDS             complement(3845..5230)
                     /gene="cysS"
                     /locus_tag="OO468_003278"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR6260284.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5404..5898
                     /gene="ppiB"
                     /locus_tag="OO468_003279"
     CDS             5404..5898
                     /gene="ppiB"
                     /locus_tag="OO468_003279"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR6260285.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            5901..6623
                     /gene="lpxH"
                     /locus_tag="OO468_003280"
     CDS             5901..6623
                     /gene="lpxH"
                     /locus_tag="OO468_003280"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR6260286.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            6741..7250
                     /gene="purE"
                     /locus_tag="OO468_003281"
     CDS             6741..7250
                     /gene="purE"
                     /locus_tag="OO468_003281"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR6260287.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7247..8314
                     /gene="purK"
                     /locus_tag="OO468_003282"
     CDS             7247..8314
                     /gene="purK"
                     /locus_tag="OO468_003282"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR6260288.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8451..9344)
                     /locus_tag="OO468_003283"
     CDS             complement(8451..9344)
                     /locus_tag="OO468_003283"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR6260289.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9341..9988)
                     /locus_tag="OO468_003284"
                     /pseudo
     CDS             complement(9341..9988)
                     /locus_tag="OO468_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            9991..11223
                     /gene="allC"
                     /locus_tag="OO468_003285"
     CDS             9991..11223
                     /gene="allC"
                     /locus_tag="OO468_003285"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR6260290.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11234..>11589
                     /locus_tag="OO468_003286"
     CDS             11234..>11589
                     /locus_tag="OO468_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HCR6260291.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMT"
BASE COUNT         2797 a         3013 c         2977 g         2802 t
ORIGIN      
        1 gtgccattct ctaataaaaa tgccgggttg aaataacttt ccgcatagct taaaaaggag
       61 ggtaaaatgg caagagatga ataacaaatt ccggccagac accaggtata cctctgcgga
      121 atatccgtag tagtgggtat tttcataatt agctctgata ttgtcagatg attcggttag
      181 tttaagttca cttctctgac cggggttact gaaccataat cattaacggt ctgaaatttc
      241 agctttccct gcactcctga gggtaaggga atttgtgaac taactctggg tgccgccata
      301 gcattcccca acttttggct gcccacttgc aggttaacga gggtgacata aaaaggtgat
      361 ggattatgaa cattgatata gttaccggat cgcgaaaact tgagtgtgtc cggcgcttct
      421 gcgggtaatt cttgtaattg aattggacgg agaaataatt tcatgcgcga taaaatcgcc
      481 agttgcaaaa cattcctgcc gttcaatgca tttttatcta ccgaagggat cgtcttaaca
      541 ttcatccaga acagagactc acgatctgcc gccagcggtg gaccggtgta aataatacgc
      601 aaagtatttt cgcttttggg ttcactaaca aatagtggcg gtgtaatgat gaatgacttt
      661 tcttttacac cgctgctgtt ttcaatccac gaattgacca gaaagagctc attggtatgg
      721 ctatttttaa tccataccgc agtctgttta gcatcagcgg gataaataat acgcgtggca
      781 cctaatgcga tccctccggc agcatgggcg ctggccgaaa tgattaaata aaatatcatg
      841 agcatcaata actttatttt agtcatcata gttaatacgc cttgtgtatt cctgacggca
      901 aaaataagga gtataaagaa atgattgctt tggcttctta caacacatca ttcccttttc
      961 atttaataaa gcggcatgat acaaagtgaa cgtggtttcg atttacattg ccaccatacc
     1021 gcttcagaca accggtacat tactttgtta taagacgtga tatataattc aaaacaacgt
     1081 ggttttgatt attcgtatct cataataaaa gtcgcgtcag catttgcttg ccctgctgat
     1141 gcacttgtac cggtgccttt ataacgtgca gaaaaatgaa gaacgttggt cccggggatc
     1201 aagttctggt tggttgagaa gctattccca tcaggtttga gaattgcgga tgtattatca
     1261 agtatttcaa taccgacacc cgttgctgtt gttgtatttg tactggatac aatggccaat
     1321 aaattatcat tgattgcatc agcctgacca gaaaatgcaa cggcagcatt agcggcaaca
     1381 acaggatcgc agtcgttcaa ctgaatggtg aatggaatta atgcagaggt attaccaaca
     1441 gcattgaaaa tatcggtacg atattgaccg agtgtgacaa cctggtctgc cgagtcagta
     1501 ttcactgaac aggcagcatt caccagttcg ccttcaaagt gaattgtacc gccatctaca
     1561 acggcagcat aactacccgt agcggcgaat agtgcggcag ccagcgcaga cgaaataaat
     1621 cttaatttca tatatattcc ttcaatctca tttatcgact ccacatccgt atataaccga
     1681 ttactttatt taagacactg atagtagtaa aattcctttt tatcctctaa gaatgtctta
     1741 attgaaaata tgcactctat tctaaaaaat agagagcccc gttagatgaa tacttccgcg
     1801 caaaatatat tcaccacaaa tatagacctg aagcggtaaa ttaccaggct gaaaattctt
     1861 tttatattgt caggtatttc ttaaattatc ttaatcctta aacaaggaaa taaatcagtt
     1921 ccagatttac aacgccatca tgtacgaaaa atgaagcttt caatctcagc gacctcagcg
     1981 acggtgcgcc taaccttcgc aagaggtcgc ttcacgcgat aaatctgaaa cgaaacctga
     2041 cagcgcgccc cgcttctgac aaaatagacg catccccttc gacctacgta acagatggaa
     2101 tcctctctct gatggcagca aagattattg acggtaaaac gattgcgcag caggtgcgct
     2161 ctgaagttgc tcaaaaagtt caggctcgta ttgcagccgg actgcgggca ccaggactgg
     2221 ccgttgtgct ggtgggtagt aaccctgcat cgcaaattta tgtcgcaagc aaacgcaagg
     2281 cttgtgaaga agtcgggttc gtctcccgct cttatgacct cccggaaacc accagcgaag
     2341 cggagctgct ggagcttatc gatgcgctga atgccgacaa caccatcgat ggcattctgg
     2401 ttcaactgcc gttaccggcg ggtattgata acgtcaaagt gctggaacgt attcatccgg
     2461 acaaagacgt ggacggtttc catccttaca acgtcggtcg tctgtgccag cgcgcgccgc
     2521 gtctgcgtcc ctgcaccccg cgcggtatcg tcacgctgct tgagcgttac aacattgata
     2581 ccttcggcct caacgccgtg gtgattggcg catcgaatat cgtcggtcgc ccaatgagca
     2641 tggaactgtt gctcgcaggt tgcaccacca ccgtgactca ccgcttcact aaaaatctgc
     2701 gtcatcacgt agaaaatgcc gatctgttga tcgttgccgt tggcaagcca ggctttattc
     2761 ccggtgactg gatcaaagaa ggcgcaattg tgattgatgt cggcatcaac cgtctggaaa
     2821 atggcaaagt tgtgggcgac gtcgtgtttg aagacgcggc taaacgcgcc tcatacatta
     2881 cgcctgttcc cggcggcgtt ggcccgatga cggttgccac gctgattgaa aacacgctac
     2941 aggcgtgcgt tgaatatcat gatccacagg gtgagtaaca tggcgacatt ttctttaggt
     3001 aaacatccgc acgttgagct gtgcgacttg ctgaaactgg aaggctggag cgaaagcggc
     3061 gcgcaggcga aaatcgcgat tgccgaaggc caggtgaaag tcgacggtgc ggttgaaacg
     3121 cgcaaacgct gcaaaatcgt cgccggtcag acagtgagtt ttgcaggaca cagcgtacag
     3181 gttgttgcct gatccctttc ccggcctgta tcaacaggcc ggttctcttt tcagactttt
     3241 gccctctacg ccccgttcat ccatgctgat agcatcgaca ttttgccgaa gcgaccatca
     3301 tgccaaccgt tattacgcac gccgccgttc ccctttgcat tggtttaggg ttgggttcga
     3361 aaggtctgtt atttgccgga atcatcctgg cgatgctgcc cgatgccgac gtattgtcgt
     3421 ttaaatttgg cgttgcttac ggcaatgttt ttggtcatcg cggttttacc cattcgctgg
     3481 tgcttgcgtt tgttgtcccg ctcttatgtg tgctgattgg acgtcgatgg ttcagggctg
     3541 ggctgattcg ctgctggctg tttttaaccg tctcgttgct atcgcacagc ttactggatt
     3601 cggtaaccac tggcggtaag ggcgttggct ggctgtggcc gtggtcagat gaacgctttt
     3661 tcgctccctg gcaggtgatt aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt
     3721 acgggcatca ggtgattatt tccgaattga tgtgggtgtg gttgccgggg atgctactga
     3781 tgggaatgtt gtggtggcgc agacgataac cggatgcgaa aactcgcatc cggcaattgc
     3841 gcaattactt acgacgccag gtggtccctt gcgggccatc ttccagcacg atccccatct
     3901 cgttaagacg gtcacgtgcc gcatctgccg ccgcccagtc tttcgcttta cgggcatcca
     3961 gacgctgttg aattaacgct tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc
     4021 tttgcaggaa cgcttccggt tcttgctcca gcaggcccaa tacagcggaa agtttacgca
     4081 ggtgagatgc cattgcattc gccgctgcca tatcttctgc tttcagacgg ttcacttcac
     4141 gcgccatatc aaacagcacg gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt
     4201 caataaagcg cgcttcaaac gcttcgccac cggcaggcgc aacggtttta tctgtgccgc
     4261 gcagcgcagt gtagaggcgc tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt
     4321 agttcagctg gctgcgatag tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt
     4381 agtatttcag cacatcgcgc acggtaaaga agttacccag cgatttggac atcttctcgc
     4441 ggtcaaccat caccattccg gagtgcatcc agtagttcac atactgacca tcatgggcac
     4501 aggtggactg cgcgatttcg ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt
     4561 ggatatcaaa gtggttaccc agctgcttgc agttcatggc cgaacattca atgtgccagc
     4621 caggacgacc cgcgccccac ggagacggcc agctcggttc gccctctttc gacatcttcc
     4681 acagaacgaa gtccatccgg ttgcgtttgt catcaaccac gtcaacgcgc gcgccagcct
     4741 gcagctggtc gagatcctga cgcgacagca cgccataagt tggatcggtc gggacgtcga
     4801 acatcacgtc gctgttgtcc gccacataag cgtgaccttt ggcgatcagt tgttcagtga
     4861 gttcaataat ttctgcgata tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt
     4921 tcaaagcatc aaaatctttg tgcatttcgg cgatcatgcg atccaccagc gccacaaagc
     4981 tttcgccatt ttcattggcg cgtttgatga ttttgtcgtc gatatcggta atgttgcgca
     5041 catacttcag tttatagccg aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa
     5101 aggtacgccc gtgaccgata tgacagagat cgtaaacggt gattccacac acgtacatgc
     5161 cgacttcccc ggcgtgaata ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga
     5221 tttttagcat cgaagattcc gtttagacat gtgtgggtaa ttgagttgcg tataataccc
     5281 atatttcccg cgcgaatcag catacattgc gtgatgatcc gatcgtgcgg ttatgctata
     5341 acaccaccct atatatgacc cgaactgggt tgaagcacca atcaaacgga acaggatgca
     5401 aaaatggtta ctttccacac caatcacggc gatattgtca tcaaaacttt tgacgataaa
     5461 gcacctgaaa cagttaaaaa cttcctggac tactgccgcg aaggttttta caacaacacc
     5521 attttccacc gtgttatcaa cggctttatg attcagggcg gcggttttga accgggcatg
     5581 aaacaaaaag ccaccaaaga accgatcaaa aacgaagcca acaacggcct gaaaaatacc
     5641 cgtggtacgc tggcaatggc acgtactcag gctccgcact ctgcaactgc acagttcttc
     5701 atcaacgtgg ttgataacga cttcctgaac ttctctggcg aaagcctgca aggttggggc
     5761 tactgcgtgt ttgctgaagt ggttgacggc atggacgtgg tagacaaaat caaaggtgtt
     5821 gcaaccggtc gtaacggtat gcaccaggac gtgccaaaag aagacgttat cattgaaagc
     5881 gtgaccgtta gcgagtaatc gtggcgacac tctttattgc agatcttcat ctctgcgtgg
     5941 aagaaccggc gatcaccgcc ggttttctgc gttttttagc gggggaagcc cgcaaggccg
     6001 acgcgctgta tattcttggc gatctgtttg aagcatggat tggcgacgac gatcccaacc
     6061 cactccatcg ccagatggcg gcagcgatca aagcggtgtc cgattccggc gttccctgtt
     6121 atttcattca tggcaaccgt gattttctgc tcggcaaacg ctttgcccgt gaaagcggca
     6181 tgacattatt gccggaagaa aaggtgctcg aactttatgg tcgccgggtg ttgattatgc
     6241 atggcgacac gctgtgcacc gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca
     6301 aaccctggct gcaaatgcta ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg
     6361 cgcgaatgcg cgcgaacagc aaagaagcca acagcagtaa atcgctggcg atcatggacg
     6421 ttaaccaaaa cgctgtggtc agtgcgatgg aaaaacatca ggtgcaatgg ctaatccacg
     6481 ggcataccca tcgcccggcg gtgcatgaac ttatcgccaa ccagcaacct gcttttcgcg
     6541 tggtactggg tgcctggcat acggaaggtt cgatggtgaa agtcacggct gatgacgttg
     6601 agctgattca ttttccgttt taaaaaaccc acaattttgc tgatttcaca gccacgcaac
     6661 cgttttcctt gctctctttc cgtgctattc tctgtgccct ctaaagccga gagttgtgca
     6721 ccacaggagt tttaagacgc atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga
     6781 tggggtccaa aagcgactgg gctaccatgc agttcgccgt cgaaatcttc gaaatcctga
     6841 atgtcccgca ccacgttgaa gtggtttctg ctcaccgcac ccccgataaa ctgttcagct
     6901 tcgccgaaag cgccgaagag aacggttatc aggtgattat tgcgggcgca ggcggcgcag
     6961 cgcacctgcc aggcatgatt gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac
     7021 agagcgccgc actgagcggt gtcgatagcc tctactccat cgtacaaatg ccgcgcggca
     7081 ttccggtggg tacgctggcg attggtaaag ctggcgcggc aaacgcggca ttactggcag
     7141 cacaaattct cgcgactcac gataaagagc tacaccagcg tctgaatggc tggcgcaaag
     7201 cccagaccga cgaagtgctg gaaaacccgg acccgcgagg tgcggcatga aacaggtttg
     7261 cgtcctcggt aacgggcagt taggccgtat gctgcgtcag gcaggcgaac cgttaggcat
     7321 tgctgtctgg ccggtcgggc tggacgctga accggcggcg gtgccttttc aacaaagcgt
     7381 gattaccgct gagatcgaac gctggccgga aaccgcatta acccgcgagc tggcgcgtca
     7441 tccggccttt gtgaaccgcg atgtgttccc gattattgct gaccgtctga ctcagaaaca
     7501 gcttttcgat aagctccacc tgccgaccgc gccgtggcag ttacttgccg atcgcagcga
     7561 gtggcctgcg gtgtttgatc gtttaggtga actggcgatt gttaagcgtc gcactggtgg
     7621 ctatgacggt cgcggtcaat ggagtttacg cgccaatgaa accgaacagt taccggcaga
     7681 gtgttacggt gaatgtattg tcgagcaggg cattaacttc tctggtgaag tatcgctggt
     7741 tggcgcgcgc ggctttgatg gcagcaccgt gttttatccg ctgacgcata acctgcatca
     7801 ggacggtatt ttgcgcacca gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca
     7861 agccgaagag atgctgtcgg cgattatgca ggagctgggc tatgtgggcg tgatggcgat
     7921 ggagtgtttt gtcaccccgc aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa
     7981 cagcggtcac tggacacaaa acggtgccag catcagccag tttgagctgc atctgcgggc
     8041 gattaccgat ctgccgttac cgcaaccagt agtgaataat ccgtcggtga tgatcaatct
     8101 gattggtagc gatgtgaatt atgactggct gaaattgccg ctggtgcatc tgcactggta
     8161 cgacaaagaa gtccgtccgg ggcgtaaagt ggggcatctg aatttgaccg acagcgacac
     8221 atcgcgtctg actgcgacgc tggaagcctt aatcccgctg ctgccgccag agtatgccag
     8281 cggcgtgatt tgggcgcaga gtaagttcag ttaagcaggt gctcatttga ttgccggatg
     8341 cggcgtaaac gccttatccg gcttaccgct ccggtaccca ttgtaggcct gataagacgc
     8401 gtcaagcgtc gcatcaggca tggtgcaccg attgccggat gctctcatca ctacagcgaa
     8461 atacaggtcc ccgcttcgcc cgccagcgtc tcttcaattc gcgataacgc cccaatccac
     8521 gcgggtttac tacggcttct gacataacca ctcaccgccg ttaccttcgg ccccatcgaa
     8581 ccatcggctt tggcaaatgg cgctaactca tccggtgtgg catggcgaat ggcacgttgc
     8641 tgcggcgttc cccagttttc atataccgca tcagcatcgg tgaggatcac cagtccatcg
     8701 gcattaatct gctcggcgag caacgcagcg gcgagatctt tatcaatcac cgcttcactc
     8761 cctgccccat cttccgtcac aggcacaccg ccgccgccac tgcaaatcac cacatgcccc
     8821 tctttgagca acaactcgat ggcttcgctg tcgagaattt tacgcggttg cggagacgcc
     8881 accacccggc gcaaatattt accatcacgt ttcatctgcc agccgtaagc cgcttccagt
     8941 gcctcttgtt cttctggctg ataaaccgga ccaataaatt tctctggctg caaaaacgcc
     9001 ggatcatcag gcgaaacctc aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc
     9061 tgtgcgctca agctctgcgc cagcatatag ccaatcatcc cctggctttc cgccaccagt
     9121 acatccagcg gatacggttc tacctctttc cacgccagat tctgtaacgc cagcagcccc
     9181 acctgcggcc cgttgccgtg aacaatcgcc agccgataag aacgggccag gcgtgccagc
     9241 gcgggtacag cactggcgat attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc
     9301 tggagtaagg cgttgccccc aagagctaca accagtgttt tcatggtttt ccttgtaata
     9361 attgttggcc aagccagaac cccagcagcg tatctgcccc tgacgtatgc cccagcgcca
     9421 gcagcgaatc aatcgcaaca gcggtacgtt tcgggcaact cagagcatgg acaaagtgca
     9481 ggagtggcga ggcgaaatat ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg
     9541 tggtaacgag ttgaagattg tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta
     9601 aagcgccata ataccaggcc gccagcagca ttccgctcaa cgtgtcatca tgacttggcg
     9661 ttaatccggg gcctttaccc agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg
     9721 cctgaaaaca gtgacgaaac tggcgtaatt cagcgggcag cggatcgctt gccgccatcg
     9781 ccagtggccc gaaaagtccg gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt
     9841 gaggatgcga aggcggcgta atacgcagca aacaataacg ctgtggctgt ttaaccgtga
     9901 aacgcccgag gcgaatccct tgagccacaa cctgtggtcg ttcattgccg cataatccac
     9961 cgcataacgc atcgaattgc gtgcggcgaa attacacatt cccgtcaagc tatagaagaa
    10021 acgctgccct ggctttcctc ttttggcgct gacccaacgg gtgggatgac ccgtttactt
    10081 tattcgccgg aatggctgga aacccagcag caatttaaaa aaagaatggc agcaagtggg
    10141 ctggaaacac gtttcgatga agtggggaat ttatacggtc gcctgagtgg caccgaatat
    10201 ccacaggaag tggttctgag cggttcgcat atcgataccg tggttaacgg cggtaacctc
    10261 gacgggcaat tcggcgcgct ggcggcgtgg ctggcaattg actggttgaa aacgcaatac
    10321 ggcgcaccgt tacgtacggt cgaagtggtg gcgatggcag aagaagaagg cagccgcttc
    10381 ccgtatgtct tctggggcag taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg
    10441 aatatctgtg atgccaaagg aaatagtttt gccgatgcga tgaaggcttg cggatttact
    10501 ctgccgaacg ccccactaac tccgcgtcag gctataaaag cctttgttga actgcatatt
    10561 gaacagggct gtgtgctgga aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc
    10621 gggcagcgtc gctatacggt gacgctgaac ggcgaatcaa accatgcagg caccacgccg
    10681 atgggttatc gtcgtgatac ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa
    10741 aaagcgaaaa agatgggcga tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg
    10801 aatacggtaa atgtggtgcc gggtaaaacc acgttcacca ttgattgtcg tcataccgac
    10861 gctgccgtgc tgcgcgattt cacccaacag ttagaaaacg acatgcgggc gatttgcgat
    10921 gaaatggaca ttggtattga tatcgattta tggatggacg aagaacccgt gccgatgaat
    10981 aaggagctgg tcgccaccct gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg
    11041 atgcacagtg gtgccgggca cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg
    11101 atttttatcc ccagcatcaa cgggatcagc cataacccgg cggaacgcac caatattact
    11161 gaccttgccg aaggggtcaa aacgttggca ctcatgcttt atcaacttgc ctggcaggaa
    11221 taaggagtca taaatgggat atttaaataa cgtcaccggt taccgcgaag atttactggc
    11281 taaccgtgcg attgttaaac acggtaattt cgcactgtta accccggatg gtctgataaa
    11341 aaatattatt ccggactttg aaaattgtga cgcgacaatc ctctccacgc caaagctggg
    11401 tgcctctttt gttgattatc tggtcacact gcatcaaaac ggtggcaacc aacagggctt
    11461 cggtggcgaa ggcattgaaa cgttcctgta tgtgatctct ggaaatatca ctgccaaagc
    11521 cgaaggcaaa acatttgcct taagcgaagg tggctatctt tattgcccac caggctcctt
    11581 aatgacgtt
//
LOCUS       DAJTUP010000112        11560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTUP010000112 DAJTUP010000000
VERSION     DAJTUP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.112"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            174..250
                     /locus_tag="OO468_003287"
     tRNA            174..250
                     /locus_tag="OO468_003287"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Arg,seq:tct)
     gene            complement(296..1057)
                     /gene="envY"
                     /locus_tag="OO468_003288"
     CDS             complement(296..1057)
                     /gene="envY"
                     /locus_tag="OO468_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR6260292.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1240..2130)
                     /locus_tag="OO468_003289"
     CDS             complement(1240..2130)
                     /locus_tag="OO468_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR6260293.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2131..5099)
                     /gene="nfrA"
                     /locus_tag="OO468_003290"
                     /pseudo
     CDS             complement(2131..5099)
                     /gene="nfrA"
                     /locus_tag="OO468_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            complement(5086..7323)
                     /gene="nrfB"
                     /locus_tag="OO468_003291"
     CDS             complement(5086..7323)
                     /gene="nrfB"
                     /locus_tag="OO468_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR6260294.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7473..8915)
                     /gene="cusS"
                     /locus_tag="OO468_003292"
     CDS             complement(7473..8915)
                     /gene="cusS"
                     /locus_tag="OO468_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR6260295.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(8905..9588)
                     /gene="cusR"
                     /locus_tag="OO468_003293"
     CDS             complement(8905..9588)
                     /gene="cusR"
                     /locus_tag="OO468_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR6260296.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9745..11127
                     /gene="cusC"
                     /locus_tag="OO468_003294"
     CDS             9745..11127
                     /gene="cusC"
                     /locus_tag="OO468_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR6260297.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            11151..11483
                     /gene="cusF"
                     /locus_tag="OO468_003295"
     CDS             11151..11483
                     /gene="cusF"
                     /locus_tag="OO468_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR6260298.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
BASE COUNT         2772 a         2968 c         3013 g         2807 t
ORIGIN      
        1 acgggttttc ttgattcaga cgcgcagcgg tgtgcgtttg tttgccgcta tagcgaaata
       61 aatcagaaaa tcagacgcgg tcgttcactt gttcagcaac cagatcaaaa gccattgact
      121 cagcaagggt tgaccgtata attcacgcga ttacaccgca ttgcggtatc aacgcgccct
      181 tagctcagtt ggatagagca acgaccttct aagtcgtggg ccgcaggttc gaatcctgca
      241 gggcgcgcca tttcttcttc atttatgccc gtcttatccg tttccgcttt gcccttcacc
      301 acatcacttt ttgtcgctgt ttggcgagat aattcaacgg tgtcaggctg taaaacgcct
      361 taaaaacaga gataaagtac gacgtgctgc tatagccaca taattgcgcc acctgagtga
      421 tatttttgtt atccatcaat aacatctgta cggcgtaacg catacgacac tctgtgacaa
      481 tctggctata gctggtattt tcgtttttta atttcttttt gagcaggctg gggcttaaac
      541 atagcgaact ggcgacaatt cgcagattcc agtaatgctg aatatcgctt tgaataatgc
      601 ggcagacgct gtcgcggacg ctgctgcgta agatatacat cagtagggca ataaaccgcg
      661 attgctcaag aaagttagac aatacggtaa aaagtaatgc gcgcgtcaac tccgcttcgc
      721 agggtttgct ggattgctgc ttgctgtggt tggtggctag ccggaacact tccggtgtac
      781 ggctatgaca agcgataacc ggggttgcta gccgcgacca gggctttaca caggttaatt
      841 tcttatttaa aaacaacaga taatcgttga tgatgttacg gttgatgtgg gtgattaata
      901 cgtgatttaa cgttgagagt tcaatgacgt tattgttgca cgcgaaggcg gccaggtagt
      961 ttttcggaag ggtaaacgta gcatggttat tgacgcttac ctctacctct ttttcggtca
     1021 ggatcaccac gcaaggttca ctgctgctca attgcatttc gcactcctca gatatcagaa
     1081 actccgctca aaggatctat gcttcctgca tgagtgatcg gcccgttcgc cgataacgat
     1141 cttctttctt tagcatgctt tttagcaatt aatcttgatg gaattctgac gagagcgaaa
     1201 gaggtaagcc aggtcgtacc cgacttacct ggaggggatt taatactcga gaatgccgtg
     1261 cgcgacgggc aaatagcgca gagagaaata gagcatgtct ttaccgcaag aggaacgttt
     1321 cgctaacagc gaggcaattt ctgcgtccgg tttcggtttc gctgtaaagg ttttgccttt
     1381 gccggcgaca aacagttcat aaacaatgcc gcttgctggg gcggagcttt ggcaatcggc
     1441 gctggcctgt agataacgtt cacgctgttc agcggtaagt ttatccacgc cgctgccatc
     1501 ctgtacccag acattaactc cggttgcttt aacttgttcc agcagttggc gatagccatc
     1561 gggcgacatg tttccggcga aaaaactact gatataaacc ggttttgctg aaacatcgct
     1621 aatcagccgc tgcgcgttgt ttaaccatgt tagcaaaggt tgacgggcgg cttcgctgcg
     1681 ccagttcagg tcgtcaattt ccgcgctgat gtaccagcca tccggcgtta tgccaggcgc
     1741 ggcgctccat aatctggctt gctggagatc ggcagccagc aggcgattaa gatagctttc
     1801 cagcgctgca gacgactgtt tctggtgcat aaaaaattcc ggatcggcgt tcagcccgac
     1861 aatcagcttc aggccagtct gttgcgcagc tgcggcacgc ttaaacaata acgtgcgctg
     1921 ttctggctgg gtaaatgcat cgccgtaacg ggtccattgc aaaaccaggg tatcgaagcc
     1981 ttgaaaacgt aactgactca tcagcccctg ccactgggta tcggtaacct gactatctcg
     2041 gttttgtggt tgccagataa tacctttcat cgcaaaggaa aaagggctga ccaaaagcag
     2101 tgtcagcaat acgaaaatga acttacgcat ttaccagtgc actccaatgg tgagaaacgc
     2161 gttgttgcgc tctccgttac gttgattaat cgccttaaag gtatgttgat actcgacgcc
     2221 gagactgact ttgtgcggcc aggcgtcgta gtgcgtctcg ccggtccaaa tattccagcg
     2281 gaccccgaca ccgccaagct gcgcgccctg agtgccttta tcacgatagc cgttgtcctg
     2341 aacgtgagcg taaggctcaa tagtctgtcc gttagctacc ttctgatgcc agctgacgcg
     2401 ataatctgcc gtccacgcct gaatatcctg gcggatatat tgcgccgcat cgaggtacag
     2461 gttttgggca aaccagcctg aaccgttcgg gtgccattcg tcgctgtatt tgccgccatt
     2521 aaagaatgag gcgctggcgc gcagcatggt atcggatgcg ccattttgcc cgttcagcgg
     2581 caactgctgt tcgacggcga ggaaaaagat ctgatcgcgc agcggcttcc agcgcagacc
     2641 ggtgccggac atcggatttt tcaccggcat catcaccccg ttttctccgg tatcggcaaa
     2701 gacgcggcta taaactgaga gcaggtcgcc ttccagcagc atattgcgtc cgatgcggta
     2761 ctcggcttcc agttgtccgt agctacgata gcttttccca ggcgctgcac cgccgacatt
     2821 attgttagcg gtactcattg cgccggaacg caagccgatg gaagaatcga aactgaacgt
     2881 ccagcggcga ccgacctcct catgcaaacg gcggaaattg aagcgttgct gattttgctc
     2941 tggggtcagt ggggttatgc ctgattatca atgtcatcaa tcacgagccg ggcgtagtgc
     3001 tgcgtcgcag gcatgtcatc cagacgctgg ttcacgtagg ccagttgtcg gatcagtgcc
     3061 ggatcgtccg gaagcccttt atgcgcctgt tcgagcattt cccgcgactg tgcgatatca
     3121 ccgctatccc acaaggcgta accaagcgct gcctgggtgt tgctattatt tggttccagt
     3181 tccagcgcgg cgcgcaaatc actcaccgcg gccggaacat tatgacgttg gcgataaatt
     3241 gtcgcccgcg caacgtaaga gttggcagaa ggggcaatat tgattgagcg cgtgagatcg
     3301 ttcagtgcga gttctggctg agctgggata taacgtcgcg catgcagcca ccagtattga
     3361 gcattgtttc ccagctcacg ttgttctgcc tgttgaagcc agcgatcgcg agctgcgcca
     3421 tttcccgctg cctgagcggt gttggcggcg gcgagcagat catcattgct catgtcgtga
     3481 agactgattt tctgccaggc cgccagtgcg gtggcgtagt cctcaacctg atacgcctga
     3541 taggctaccg cacgatgttg ccaggcgctc ggttgtcgtt gttcggcctg aagccatgca
     3601 tacaacgcca caccgggtag cgtgtcccga taacactttg ccagacggtt ccaggcggca
     3661 gcatcgtagg aaggcgacat atcgcccagc aagcgaacta ttgccgggca attatctgca
     3721 atacccggca actgactttg ccactgacgt tgctccgcca gcggtaaggg tttcgataaa
     3781 atcgccacct tcgccggcgt tgctaggtaa ggatgacttt ccagcagaga cgccagtcgc
     3841 gccattaaag tctggctgac acgcgcatcg ccctggaaag gatagcgttg cagcaataaa
     3901 tcggcagctt cgcgtgactg ctcgttctgc atcagttgcc aggtcagttg atccaggcgg
     3961 ggaagatttg tcggttcttg ctgatacagc aatcgtgcca gacgcagaga ttcagccttg
     4021 ttacgggtcg ccacgctgac agcataacgc tcctcaagca tttcattggc ggggagggtg
     4081 gcgagcagtt tttgcgctgc gtcgtactga ccttctttta acagcaccgg tagcgtcgcg
     4141 ccaacaacat actggcggtt atcggcaaac tgcaccgtat aattcgccaa tgcctgaacg
     4201 gggttggcgc tgtatttaga taacagatag agccaacttt tctcttgtgc gcccgtggta
     4261 aacagcggct tattttcaat gagataatgc tggaggcgtg ctttttcgcc acgataagcc
     4321 agcgcggtcg cgtaagtaat atatgactga ggatcggtga agatcccctg tgattgcagt
     4381 gccaggatcc gatcgtccag ctgcccggca agaagcacgt caaaccactg acggtgttct
     4441 gccgcgctta atgtgttctg ctggcgtgct tcattgtata gcgtatctgc ctgggatcat
     4501 tgtttcaggt agattgcccg ttgcagcaga tcggttcgca gcgtttttcc ttccggcgat
     4561 gcagcaaacg tcgcatcgtt cagttgcgct ctggcgacag gtaattgtgc cagccgcagg
     4621 gcattctgcc cgacttcact gcgacaacgc agggtcggcg cagcgtcgcc cgctttttgc
     4681 tgggcaagca gttcttcaac agtcgtaacg cttttcactt caactggaat agccgccaga
     4741 ctgcgctcaa gtcgggcatc tcctgggtga cgtttcagtt gatcctcaag caacagccgc
     4801 gcccggtcat catgaccaaa atgacgatag gcttccgcaa ggtataaagt cagcggaata
     4861 ttatccggca cctgttggtg tatatattca aattcgcgga tggcggttgc ttcgtcgtta
     4921 tttttctgtg ccttcagcgc cttatcgaga cggggataaa taacaaaatg gcgataatcg
     4981 ctcagcccca gctcttctgc gctggtgccg atattgtctg cgagtgcgct ggtactcaat
     5041 aaagacgtca gcagtaaacc agaccatccg atgacgcgat taaggttatt ctccttcatt
     5101 ttcggactcc agttgcgcaa cctgttctgt gtttaaacct gctttgagta atagtgattg
     5161 catcgaaact tgtaattcgc gttgaattgt caggacgcga tccaacgttt cctggctgat
     5221 aacgccttcg gtgaccaaaa acttgccgag cggcagagaa ctgcgttcat ggcgcaataa
     5281 caacacgtta attgctgaac gattaatatg accgagcgtg gtcagtattt cggcgaacag
     5341 gaactgatgc ggcacatatt gccgccagat ttcaccggtc tgctgttccg tgagccactg
     5401 atgctgaacc gcattgtaca acattgcccg cggatcgtga ccgcgtcggc gtgcatacca
     5461 gtggcgtaac cctgtgacaa tttgtccccg cagaacaatg acgtaacgca ctttgcgtcc
     5521 gactttacgc gtcagggccg ccagcgaaac cgggtcaata ccatcttcac tgccgacaat
     5581 taactcatca ttttccagac gcagcggcag tactgcataa tgcagcgcta cggaggctgg
     5641 catttcggca atcagcgagg acgggatctg ccaggcatcg atggattccc acgccacgcc
     5701 gttttgctct gccagcgcct gtgccagctg ctcggcgcta atcagcccct gcatcagcat
     5761 tgaaccgccc aggcgtagac cttcgacgcg attacgcagt gctgtatcga gttgttcttc
     5821 agtgatgacc tgattttcca gcagaatttg acctaacggg cgcaacgagc gggtatcgcc
     5881 agtgacgctg gggaagtcat gcgttgtttt atcccacgcg acgcgacgcg gatcgccgtg
     5941 ctgaagaacc tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa
     6001 aagacgcagg acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac
     6061 tcgctgcacg atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca
     6121 tgcgctgccg ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc
     6181 cagcaacagc aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat
     6241 tgcccctttg cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg
     6301 ggttttaaag ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt
     6361 cgaaaaggta tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg
     6421 taaaaattta cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat
     6481 ttccgtcata cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg
     6541 cacgtcgaaa gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa
     6601 acaggtgccg acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc
     6661 tttgccatgc agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc
     6721 gcgttcaaac ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa
     6781 cagacgcaat tccatcggtg aaatcacatc ttcggcgtca tgcagaataa aaccagcaaa
     6841 agcgaaattg gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcaggcagtc
     6901 ggctttgctg gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc
     6961 gcacacttcg tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat
     7021 atgatagttt tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc cgatgacgcc
     7081 cgtttcattc cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc
     7141 gcggtaactc attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac
     7201 ccagtagacg acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa
     7261 cgttatcgcg attactttta agccgtagag ccaggtagca aaaacatcaa gaagccagtc
     7321 cacacaaaaa ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt
     7381 atgaattgtg acagcttttt accattaata tgtatgacta ttgcggcacg ttatttttac
     7441 actggttata aaagttgccg tttgctgaat aattaagcgg gtaatgtgat aacaaacctt
     7501 gtcccccgcg catctgacgt taccgcaacc gtgcctttat gcgcgacaac aatcgatttc
     7561 actatcgcca gcccaatacc gctaccttca ccttttcgct ggcgggaggg gtcaacgcga
     7621 tagaaacggt caaacaatcg cggtaagtgc tcgggcgcaa tgggcgtacc ggggttttcg
     7681 acggaaactt gcacctggtg atcgaccgtc tgacagcgta ctacaattgt ctctctggtt
     7741 ggcgtataac gaagggcatt agaaagcagg ttgcttagcg cccgacgcag catcaacgga
     7801 tcgcccgcaa cctgacgttt gtcgccaaca aaccgcaact ccacgccgcg atcttccgct
     7861 agcgcctcga aaaaatcgaa cactttgccg acttcatccg ccaggttgag cattttcttt
     7921 tcggggatta gctggttgtt atcggcctga gcgagaaaca gcatatcgct gaccattttc
     7981 gccattcgcg tcagctcttc gagattagag tagagcacat cttccagctc cttctggctg
     8041 cgcgactggc tgagggcgat ttccgtttgc gtaatgagat tagtaattgg tgtgcgaatt
     8101 tcgtgggcga tatccgctga gaaattggac tggcgagtaa agacatcctc aatacgctcg
     8161 atcatatggt tgaacgacag taccagctgt tccagctcaa tcggcacggt ctgcgggtca
     8221 aggcgaacgt cgagatcttt cgaggtgata ttctggattt gacggctgac gctgcggatc
     8281 ggcgcgtgac ctttatgtac cgccaacagc acgataaaga cgatcaggat gctgattacc
     8341 gatgcggtca taataagttt attcatcaaa tcatttatgt aatgaagatg aaaatcgatc
     8401 gacagcgcga tgtagagcgt ataaatcggt ttgccgtcca ccaacgggcc aaccggcaag
     8461 ttaatcatcc gccagttgct gtgttccata tgcccgtgac cgtggcctgg catcataatc
     8521 gtcgggccgg aaaggagata cacctcgcca ccccgcgcgt ctttatcggg tatggcatca
     8581 cgtgcaaact cgcggatatc aggcgcaccg ggggagtgat acaccgtttt gccgtggcta
     8641 tctgccaggg aaatcaacac gttggaataa ccactgacga tatcttccag cgtcattaag
     8701 cgtcgggctt gcgtttcgtc agggtgattt agtacccgtt caagggtggc gctaatctct
     8761 tttaaatcat taatgtcctg ctcggcaaaa tgcactttta ctgagtggat tatgatccat
     8821 gcaaaggcga aaaacgccgc gatggtggcc tggctgataa aaaaggtcag gcgggttgcc
     8881 agcgaaaacg ggcgctgaaa tggcttactg accatccggc acctcaagca tgtaacccac
     8941 gccgcgcacg gtttgaatca gcttcggctc aaagtcgttg tcgattttgc cgcgtagcag
     9001 cttcaccgcc acatcaatgg cgttagtgtc gctgtcgaaa ttcatgtccc atacctgcga
     9061 ggcgataagc gagcggggca gcacttcgcc ctgatggcgg agaaaaaact ccagcagagt
     9121 aaattcttta ctggtcagag tgatgcgcgt gccgctgcgg gtgactttgc ggctgacgag
     9181 atcgaccatc agatcggcaa cctgaaactg actttcgata atcaccgccg ccccgcgccg
     9241 caggagagtg cgcacccgcg ccagcagttc agcaaaagcg aacggtttca ccaggtagtc
     9301 atctgccccc aactccagcc ccttgacgcg atgttcaatg gtgccaagcg cggtaagcaa
     9361 cagaatcggc atccctttat tggcggagcg caacatgcgc acgatatccc agccgttcac
     9421 gtctggcagc ataatatcga ggattatcag gtcataatca ccggtcatcg ccagatggta
     9481 gccattcagc ccgttgtcgg ccaaatcgac cacaaaaccg gcttcggtta accctttggt
     9541 caagtattct ccggttttct tttcatcttc gacaatcaac agtttcatat ttcctccgca
     9601 tgttgcccgg gcaattctag agtagcggga tcagatggca atcgcttatt ggcaaaatga
     9661 caattttgtc attttcctgt caccggaaaa tcagagcctg gcgagtaaag ttggcgacat
     9721 aaaatcacca gaaattatga gcctatgtct ccttgtaaac ttctgccatt ttgtgtggcc
     9781 cttgcgctaa ctggttgttc actggcaccg gattatcagc gtccggtaat gcccgtaccg
     9841 cagcagttct cactcagcca gaacggcctg gttaacgcag cggataacta ccagaacgtg
     9901 ggctggcgca ccttttttgt tgataatcag gtgaagacgc tgattagcga ggcgctggtg
     9961 aataaccggg atttgcgcat ggcgacgctg aaagtgcagg aggcacgggc gcaatatcgt
    10021 ctgaccgatg ccgaccgcta cccacagctc aatggcgagg gcagcggcag ctggagcggc
    10081 aatcttaaag gcgattcagc cacgacgcgg gagttctcga ctggccttaa cgccagcttt
    10141 gatctcgatt ttttcggtcg cttaaagaac atgagcgaag ccgagcgcca aaattattta
    10201 gccactgagg aagcccagcg cgcggtgcat attttgctgg tttctaatgt cgcgcaaagc
    10261 tatttcaatc agcagctggc gtatgcgcaa ttgcaaatag ccgaagaaac gctgcgtaat
    10321 tatcagcagt catatgcgtt tgtcgaaaaa caactgttga ccggtagcag caatgttctg
    10381 gcgctggaac aggctcgcgg ggtgatagaa agtacccgca gtgacatcgc taaacgtcag
    10441 ggggaactgg cgcaggcgaa taatgcattg caactgttat tgggaagcta cggcaagcta
    10501 ccgcaagcgc agacagtaaa cagcggcagc ctgcaaagcg ttaaattacc ggcgggcttg
    10561 tcgtcgcaaa tcttattgca gcgcccggat attatggaag ctgaacacgc gttaatggcg
    10621 gctaatgcta atattggtgc tgcgcgtgcg gcatttttcc cgtctatcag cctgaccagc
    10681 ggaatatcaa ccgccagcag cgatctgtcg tcattattta acgctagcag cgggatgtgg
    10741 aattttattc ctaaaattga gatccccatt tttaatgccg gacgcaacca ggccaatctg
    10801 gatatcgccg aaattcgcca gcagcagtcg gtggtgaatt atgaacagaa aatccagaac
    10861 gcctttaaag aggtggcaga tgcgctggca ttacgtcaaa gcctggacga tcaaatcagc
    10921 gcccagcagc gttatctggc gtcgctgcaa attaccctgc aacgggcgcg ggcattatat
    10981 cagcacggcg cggtaagtta tctggaagtg ctggatgccg agcgttcttt atttgcaacc
    11041 cgacaaactt tacttgacct gaattatgcc cgtcaggtta acgaaattta tttgtatacc
    11101 gccttagggg gcggttggca gcaataattt ttaactccag gagagaataa atgaaaaaag
    11161 cactgcaagt cgcaatgttc agtctgttta ccgttattgg ctttaatgcc caggctaacg
    11221 aacatcatca tgaaaccatg agcgaagcac aaccacaggt cattagtgcc accggtgtgg
    11281 taaaaggtgt tgatctggaa agcaaaaaaa tcaccatcca tcacgatccg attgctgccg
    11341 tcaactggcc ggagatgacc atgcgcttta ccatcacccc gcagacgaaa atgagtgaaa
    11401 ttaaaaccgg cgacaaagtg gcgtttaatt ttgtccagca gggcaacctt tctttattac
    11461 aggatattaa agtcagccag taacccaggt ttaatgagat gaaaaaaatc gcgcttatta
    11521 tcggcagcat gatcgcgggc ggtattattt cagcggcagg
//
LOCUS       DAJTUP010000113        11465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTUP010000113 DAJTUP010000000
VERSION     DAJTUP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.113"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(272..347)
                     /locus_tag="OO468_003296"
     tRNA            complement(272..347)
                     /locus_tag="OO468_003296"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(312..314),aa:Ile,seq:cat)
     gene            906..3158
                     /locus_tag="OO468_003297"
     CDS             906..3158
                     /locus_tag="OO468_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260299.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3494..4426
                     /gene="csiD"
                     /locus_tag="OO468_003298"
     CDS             3494..4426
                     /gene="csiD"
                     /locus_tag="OO468_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR6260300.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4446..5714
                     /gene="lhgO"
                     /locus_tag="OO468_003299"
     CDS             4446..5714
                     /gene="lhgO"
                     /locus_tag="OO468_003299"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR6260301.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5737..7185
                     /gene="gabD"
                     /locus_tag="OO468_003300"
                     /pseudo
     CDS             5737..7185
                     /gene="gabD"
                     /locus_tag="OO468_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7193..8479
                     /gene="gabT"
                     /locus_tag="OO468_003301"
                     /pseudo
     CDS             7193..8479
                     /gene="gabT"
                     /locus_tag="OO468_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8717..10117
                     /gene="gabP"
                     /locus_tag="OO468_003302"
     CDS             8717..10117
                     /gene="gabP"
                     /locus_tag="OO468_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR6260302.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10138..10800
                     /gene="csiR"
                     /locus_tag="OO468_003303"
     CDS             10138..10800
                     /gene="csiR"
                     /locus_tag="OO468_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR6260303.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10801..11250)
                     /gene="kbp"
                     /locus_tag="OO468_003304"
     CDS             complement(10801..11250)
                     /gene="kbp"
                     /locus_tag="OO468_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR6260304.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11334..>11465)
                     /locus_tag="OO468_003305"
     CDS             complement(11334..>11465)
                     /locus_tag="OO468_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR6260305.1"
                     /translation="IILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIHAFWVQTRD"
BASE COUNT         2941 a         2821 c         2867 g         2836 t
ORIGIN      
        1 tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcaatttgc
       61 ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga tgaataaata
      121 cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca cccaccgcac
      181 ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac cccttgtacc
      241 cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact tgaaccagcg
      301 accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta agtatgcaat
      361 aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat aacagatttt
      421 tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg acaccgcctc
      481 cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc actatctata
      541 gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat aactttatta
      601 cattagctga agagttttcg catcattatg atatctgtta cttttcactc cataaaaata
      661 aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt agtttcgtat
      721 gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac agatttaaac
      781 attatccttt tccatataga tattggataa aaataggaca tctgtttgca attactttca
      841 caacaattaa acatttttat gttttcgtat acatcatatt actctaccat tagaggaacg
      901 ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac cccacattgt
      961 tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta gaatatctaa
     1021 actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta aaagacagag
     1081 aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa aacgattata
     1141 aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca cagagtagca
     1201 ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga gatacaatgg
     1261 ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat ttcactcaca
     1321 ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt aacattcctg
     1381 actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc ggacagcaag
     1441 aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag cgtaaatctc
     1501 ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct ggcgaagcga
     1561 ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct gagtttgcac
     1621 gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc gacggaccag
     1681 aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa gatctttgta
     1741 ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa gatgctaaaa
     1801 aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc gggggaagtg
     1861 gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt aaagatatag
     1921 aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt aaagatccag
     1981 taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca aaagacttcg
     2041 aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca ttaaaagaaa
     2101 agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt atgttgcaaa
     2161 atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat gatgcagcaa
     2221 aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa aattataatg
     2281 agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc gttatgaatt
     2341 acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg ctgctttatg
     2401 aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata aaagcctttt
     2461 cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc tcaagcatcc
     2521 catcagaaag acggatattg attaacatta accacgattt tcctaacaat ggcaatcttt
     2581 tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca tatatggccg
     2641 ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa tcaacgactg
     2701 aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt aataaaggtt
     2761 gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat aaagcaggaa
     2821 tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt agtcgtgggg
     2881 attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat aaaggaaacg
     2941 aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa aacatgggga
     3001 gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct tgtaaagtat
     3061 ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct cttcttttag
     3121 ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata tctgccccga
     3181 taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg aagccattta
     3241 ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa cctgttgctt
     3301 tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt tactaatttg
     3361 ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt tcagaaatgt
     3421 agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac atctcataag
     3481 aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg attcaggcca
     3541 ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc tggaactcac
     3601 cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc
     3661 gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc
     3721 gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt
     3781 gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga agctggcaac
     3841 ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc
     3901 gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc cgcaccgcgt
     3961 gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg tgctgatgat
     4021 gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg cgcgtcgccc
     4081 gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt
     4141 cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg tccagccaaa
     4201 agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa ccagcaaagg
     4261 cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc tgttctggct
     4321 gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg
     4381 tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc aaaggaattg
     4441 agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg accgccatgc
     4501 aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg
     4561 cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat tacacgcccg
     4621 gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc ttttgcgatc
     4681 aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct cagctcgaaa
     4741 tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag cgcgagtgga
     4801 taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt ggcatttttg
     4861 tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca aaaatcttcc
     4921 aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt gagcataaaa
     4981 acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg ctgattagct
     5041 gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg ggctttatta
     5101 tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag attgttaacc
     5161 acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat ctcacccgca
     5221 tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct
     5281 atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg tcggggattc
     5341 gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac tcgctgtgca
     5401 aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta agcgatctcc
     5461 agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag ctgattgacg
     5521 attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc tccccggcag
     5581 cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa
     5641 gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc ttacacgccg
     5701 catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga cagtaactta
     5761 ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg tgaagtcatc
     5821 gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat gggcgcagat
     5881 gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc
     5941 gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca gcatcaggac
     6001 gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa
     6061 atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg tatttatggc
     6121 gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca gccgattggc
     6181 gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg taaagccggt
     6241 ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac gccgttctct
     6301 gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg
     6361 gtcaccggtt cggcaggcgt ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa
     6421 ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg cgcgaaagac
     6481 atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc
     6541 gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc
     6601 tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa
     6661 ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg cgtcaccatc
     6721 gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg
     6781 gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg caacttcttc
     6841 cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga agagacgttc
     6901 ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc acaagccaat
     6961 gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg cgtcttccgc
     7021 gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat ttccaatgaa
     7081 gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc
     7141 atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga gaatgcgaag
     7201 gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg gcgttgggca
     7261 aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg tttaaggccg
     7321 tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc tgcatccgaa
     7381 ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaggtgct
     7441 ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt
     7501 cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat
     7561 cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt atcacgggcg
     7621 cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatggggct
     7681 gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca taagcgagga
     7741 tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg aagatatcgc
     7801 cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt cgccagcctt
     7861 tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt
     7921 gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg gcgtggcgcc
     7981 ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg gcgtcaccgg
     8041 gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct atgccggtaa
     8101 cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct
     8161 gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga tagccgaaaa
     8221 acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg agctgtttga
     8281 agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg
     8341 cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc gcatccttgt
     8401 accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg gtcagtgttt
     8461 tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc gtcttgtccg
     8521 gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa
     8581 caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa aagaatcaat
     8641 aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact
     8701 ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg gcgggctgaa
     8761 atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg
     8821 ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc tgttcgccgg
     8881 attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca cgcccgatac
     8941 cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct ataccatcgg
     9001 ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca tcgccgccat
     9061 gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg tcattaccct
     9121 cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct
     9181 ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag
     9241 cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata gcggcggctt
     9301 tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt tctcgtttat
     9361 gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa aacatattgt
     9421 ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt
     9481 cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat cttatcgctc
     9541 ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg tgatattact
     9601 ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc tctactcctt
     9661 aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta
     9721 cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga actattacgc
     9781 gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc tgctggttta
     9841 tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga
     9901 aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg gctttattac
     9961 ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga tctccaccgg
    10021 cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga aaaagctggt
    10081 attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg
    10141 accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt
    10201 caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact tggcgttggg
    10261 ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa
    10321 aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc
    10381 gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga tgaatgggag
    10441 gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga cgccagcgag
    10501 aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat tgtggcgggc
    10561 tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat
    10621 cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga taaacacgat
    10681 cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc cagtgagtta
    10741 atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa
    10801 ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc tttttagcat
    10861 cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga cctgtttgga
    10921 aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag tggctggtgt
    10981 cgccgttttc acctgatcat caacactggc aataccggaa atattcccca ccgcaacaag
    11041 gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc
    11101 aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat gctcctgcac
    11161 cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga gtttttctcc
    11221 ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca
    11281 gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg
    11341 cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag tgtcagcaga
    11401 atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat cggcggcaga
    11461 atgat
//
LOCUS       DAJTUP010000114        11315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTUP010000114 DAJTUP010000000
VERSION     DAJTUP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.114"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            289..486
                     /locus_tag="OO468_003306"
     CDS             289..486
                     /locus_tag="OO468_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260306.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(436..1629)
                     /locus_tag="OO468_003307"
     CDS             complement(436..1629)
                     /locus_tag="OO468_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6260307.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1765..3489
                     /gene="iucA"
                     /locus_tag="OO468_003308"
     CDS             1765..3489
                     /gene="iucA"
                     /locus_tag="OO468_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR6260308.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3490..4437
                     /gene="iucB"
                     /locus_tag="OO468_003309"
     CDS             3490..4437
                     /gene="iucB"
                     /locus_tag="OO468_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR6260309.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4437..6179
                     /gene="iucC"
                     /locus_tag="OO468_003310"
     CDS             4437..6179
                     /gene="iucC"
                     /locus_tag="OO468_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR6260310.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6176..7513
                     /gene="iucD"
                     /locus_tag="OO468_003311"
     CDS             6176..7513
                     /gene="iucD"
                     /locus_tag="OO468_003311"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR6260311.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7519..9714
                     /gene="iutA"
                     /locus_tag="OO468_003312"
     CDS             7519..9714
                     /gene="iutA"
                     /locus_tag="OO468_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR6260312.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10265..10408)
                     /locus_tag="OO468_003313"
     CDS             complement(10265..10408)
                     /locus_tag="OO468_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260313.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10647..>11315
                     /locus_tag="OO468_003314"
     CDS             10647..>11315
                     /locus_tag="OO468_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6260314.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLL"
BASE COUNT         2833 a         2841 c         2925 g         2716 t
ORIGIN      
        1 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
       61 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
      121 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagcg
      181 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
      241 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
      301 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
      361 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
      421 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
      481 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
      541 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
      601 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
      661 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
      721 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
      781 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
      841 acccaacagc caggccacct gttggctaat ggcggtggta tccgtagcga actggcgagt
      901 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
      961 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1021 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactgggg ctgccatcag
     1081 cagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     1141 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     1201 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     1261 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     1321 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     1381 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     1441 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     1501 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     1561 cccggcactg aacgcaagtg gccagcagga cagtggtttt tggggcgtat cttcgatccc
     1621 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     1681 atctgacaat aacatttatt gttgataatg agaatcatta ttgacataat tgttattatt
     1741 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     1801 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     1861 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     1921 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     1981 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2041 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     2101 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     2161 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     2221 cctaagtctc atgaaccgtt taaccggcgg gaggctgaac gctacctgcc tgatatggca
     2281 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     2341 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     2401 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     2461 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     2521 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     2581 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     2641 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     2701 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     2761 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     2821 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     2881 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     2941 caacaggccg cccatgcatg ggttgatgca tactgtcagc aagtgctaaa gccgctgttt
     3001 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3061 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     3121 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     3181 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     3241 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     3301 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     3361 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     3421 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     3481 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     3541 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     3601 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     3661 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     3721 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     3781 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     3841 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     3901 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     3961 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4021 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4081 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     4141 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     4201 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     4261 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     4321 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     4381 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     4441 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     4501 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     4561 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     4621 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     4681 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     4741 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     4801 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     4861 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     4921 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     4981 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5041 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctgccgctg ccggtacatc
     5101 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgcc gaaggcagga
     5161 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     5221 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaatacct
     5281 catgctaccg ggggatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     5341 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     5401 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     5461 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     5521 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     5581 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     5641 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     5701 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     5761 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     5821 aggaggtgcg tgatgtcaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     5881 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     5941 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6001 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6061 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     6121 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     6181 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     6241 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     6301 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     6361 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     6421 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     6481 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     6541 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     6601 ccacaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     6661 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     6721 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     6781 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     6841 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     6901 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     6961 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7021 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaactc tggaaagtag
     7081 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     7141 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     7201 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     7261 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     7321 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     7381 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     7441 cagcggcagc cggaaaaaac cacagccgga ggctgcttct ttaactcact atacagcaaa
     7501 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     7561 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     7621 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     7681 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     7741 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     7801 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     7861 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     7921 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     7981 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8041 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     8101 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     8161 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     8221 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     8281 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     8341 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     8401 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     8461 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgacg
     8521 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     8581 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     8641 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     8701 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     8761 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     8821 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctaccggg
     8881 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     8941 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9001 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9061 aacggccatc ttcccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
     9121 aaagtcgatt cttatgaact gggctggcgc tttatcggtg acaacctgcg gactcaaatc
     9181 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
     9241 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
     9301 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
     9361 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
     9421 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
     9481 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
     9541 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
     9601 gtctggggac agcgtgcacc tctgtactac agcccgggtt acggccctgc ttcactgtac
     9661 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
     9721 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
     9781 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatgggct gttcaccact
     9841 gaataacatt atgtctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
     9901 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
     9961 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10021 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10081 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    10141 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc atcgtcagtg cccggcccgg
    10201 aggatgcccc gtagctacat acggatcagg gttggcaata tcgaatggca ggctataggc
    10261 aaagttaaag ccccatggag tagcacaaaa tatgccgcac aaaggaaacg gtctgaataa
    10321 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    10381 cgtcgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    10441 aacattaccc ctccccacat tgggcaaaaa aaaacaaaaa acgatcaatt agtattgatt
    10501 cacataatct ctcatcatta atgagaacat tgtcacatat ttacaatata gaactgttac
    10561 ttttagcaag ctgttagtaa ccaaccgtta ctaataattt taattcttaa tactttgttc
    10621 attcaataaa tgagttgaga gaatatatga ataaaatata ctcccttaaa tatagtgctg
    10681 ccactggcgg actcattgct gtttctgaat tagcgaaaag agtttctggt aaaacaaacc
    10741 gaaaacttgt agcaacaatg ttgtctctgg ctgttgcagg tacagtaaat gcagcaaata
    10801 ttgatatatc aaatgtatgg gcgagagact atcttgatct tgcacaaaat aaaggtattt
    10861 tccagcccgg agcaacagac gtaacaatca ctttaaaaaa cggagataaa ttctctttcc
    10921 ataatctctc aattccggat ttttctggtg cagcagcgag tggcgcagct accgcaatag
    10981 gaggttctta tagtgttact gttgcacata acaaaaagaa ccctcaggcc gcagaaactc
    11041 aggtttacgc tcagtcttct tacaaggttg ttgacagaag aaattccaat gattttgaga
    11101 ttcagaggtt aaataaattt gttgtggaaa cagtaggtgc caccccggca gagaccaacc
    11161 ctacaacata ttctgatgca ttagaacgct acggtatagt cacttctgac ggttcaaaaa
    11221 aaatcatagg ttttcgtgct ggctctggag gaacatcatt tattaatggt gaatccaaaa
    11281 tctcaacaaa ttcagcatat agccatgacc tgtta
//
LOCUS       DAJTUP010000115        11240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTUP010000115 DAJTUP010000000
VERSION     DAJTUP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.115"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(111..1775)
                     /gene="glnS"
                     /locus_tag="OO468_003315"
     CDS             complement(111..1775)
                     /gene="glnS"
                     /locus_tag="OO468_003315"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR6260315.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="OO468_003316"
     CDS             complement(1978..3924)
                     /gene="nagE"
                     /locus_tag="OO468_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR6260316.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            4257..5057
                     /gene="nagB"
                     /locus_tag="OO468_003317"
     CDS             4257..5057
                     /gene="nagB"
                     /locus_tag="OO468_003317"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR6260317.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5117..6265
                     /gene="nagA"
                     /locus_tag="OO468_003318"
     CDS             5117..6265
                     /gene="nagA"
                     /locus_tag="OO468_003318"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR6260318.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6274..7494
                     /gene="nagC"
                     /locus_tag="OO468_003319"
                     /gene_synonym="nagR"
     CDS             6274..7494
                     /gene="nagC"
                     /locus_tag="OO468_003319"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR6260319.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7542..8294
                     /gene="nagD"
                     /locus_tag="OO468_003320"
                     /pseudo
     CDS             7542..8294
                     /gene="nagD"
                     /locus_tag="OO468_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            8691..10355
                     /gene="asnB"
                     /locus_tag="OO468_003321"
     CDS             8691..10355
                     /gene="asnB"
                     /locus_tag="OO468_003321"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR6260320.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10734..10810
                     /locus_tag="OO468_003322"
     tRNA            10734..10810
                     /locus_tag="OO468_003322"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10768..10770,aa:Met,seq:cat)
     gene            10819..10903
                     /locus_tag="OO468_003323"
     tRNA            10819..10903
                     /locus_tag="OO468_003323"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10853..10855,aa:Leu,seq:tag)
     gene            10927..11001
                     /locus_tag="OO468_003324"
     tRNA            10927..11001
                     /locus_tag="OO468_003324"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10959..10961,aa:Gln,seq:ttg)
     gene            11036..11110
                     /locus_tag="OO468_003325"
     tRNA            11036..11110
                     /locus_tag="OO468_003325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11068..11070,aa:Gln,seq:ttg)
     gene            11126..11202
                     /locus_tag="OO468_003326"
     tRNA            11126..11202
                     /locus_tag="OO468_003326"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11160..11162,aa:Met,seq:cat)
BASE COUNT         2796 a         2901 c         2843 g         2700 t
ORIGIN      
        1 atgtaggcct gataagcgaa gcgcatcagg caatttagcg tttgaaattt attgtctatt
       61 tattgccgga tgcgacgtaa atgcctcacc cggcttggta gaacttaaaa ttactcgcct
      121 actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttcggccgtg
      181 gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca
      241 accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta
      301 atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag acgatcatac
      361 aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact
      421 ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg
      481 gtgatattac cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc
      541 agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa
      601 tcggcgcgat cgatccaaat ttcaccgcta aacggcacct gacggctccc catttccggt
      661 ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc
      721 agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg
      781 atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt
      841 ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc ggaaatggtc
      901 ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta
      961 cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcggatga
     1021 acaggaatgg tgatgttatc cagtacccag tcgtacagac gacggttgtc ctggaactca
     1081 agcgtacaca gagagtgcgt aataccttcc agcgcatcgc tgatgcaatg ggtgaagtcg
     1141 tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatgcgg
     1201 tacagcaccg gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt cgcacgcagg
     1261 caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc caagttctct
     1321 tcaacgctgc ggtcgcggta cgggctgttt ttacccggct gcgtcagggt accgcggtat
     1381 tcgcggatct gttccggtgt cagttcatca acgtacgcca ggcctttatt gatcagttcg
     1441 atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag
     1501 tgaaaaccta accactctac gtcgtttttg atcgaatcga catactcgat atcttctttt
     1561 accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg
     1621 aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg
     1681 aaacgggtgt gtactgtgat gtgcttacca ctggccagat cttcatcgat gatctgacgg
     1741 ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa
     1801 cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat
     1861 tgataatcta acaaggatta tggggatgat tgatgaaaaa aaaagcggca gagaagatct
     1921 ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc atcaggcata aagcagatta
     1981 ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag ctttaatgag
     2041 caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg agcgggcgtt
     2101 agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctta cctgcgcacc
     2161 ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga tacccatatg
     2221 gacgacgatc tccgcgcctt tttcggtttc caggcagaac gcgtggttgg tgttgaagat
     2281 tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt tcaccgccac
     2341 accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca gtgccacgac
     2401 atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta cagcctgcgg
     2461 ttttgctaca ggcgcggcag ttgccggagt tgcttcagct gacgcagcgg ctaccggacc
     2521 acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc caacaatcac
     2581 ctgaatagtc tgtttgttca gtttcactac accagaagca cccagacgtt tacacatcgt
     2641 atcgttgacg cgggcagagt cagccacggt caggcgcaga cgggtaatac aggcatcaat
     2701 cgctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca gttgagtcag
     2761 accttcttca gtattgctgt tggcttcttc agtaacgatc tcgtcttctt tatcttcacg
     2821 acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga agtagatagc
     2881 gaagaagata acgcccatca ccagcagcat ccagacgttc tggctggcgg caggcaggtt
     2941 atacatcaac gcgtagtcga tagcacccgc agagaaagag aagccggcat ggatacccag
     3001 cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt acagcagcgg
     3061 cgcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga acgcagtaac
     3121 agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg ctgcgaagta
     3181 catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc cggacatgaa
     3241 catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga aaaccgtacc
     3301 cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca cctgatgcag
     3361 accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca gcgcgcccgc
     3421 agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc agacgtaacc
     3481 aaaaatggcc gccagcacca gacagaagaa gccggtggcg atcggcacaa agcgtttgcc
     3541 gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat aggctgcgcc
     3601 accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg ggttgatggt
     3661 caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagcgccg ccgcacctgc
     3721 gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga ggttatcaaa
     3781 aattgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct gaccgaatcg
     3841 cagcaacagc gccgccaccg gcagcaccgc gatagggagc tgtaacgccc taccgagtcg
     3901 ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt tggctcgttt
     3961 aaagccgtat ttttattttg ctgcaaattt tactgccgat gttctgtaat cagattgtta
     4021 gatcatctgc tacagagtgt gtgaaaattt aattcgcatc gcaaattaaa cgcgtgtctt
     4081 ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct aaaccagaaa
     4141 acttatttta tcattcaaaa aatcaagtcg gattgacgcc tgtctgcgca aatccaggtt
     4201 acgcttaaag atgtctaatc cgccaacggc ttacatttta cttattgagg tgaataatga
     4261 gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc catatcgtca
     4321 atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt tgtactgggc ctgccgactg
     4381 gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca ggccaggtca
     4441 gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg aaagagcatc
     4501 cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat attccagcag
     4561 aaaacatcaa ccttctcaac ggcaatgccc cggatatcga tgccgagtgc cgccagtatg
     4621 aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta ggtaacgacg
     4681 gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt atcaaaaccc
     4741 tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc aatcaggtgc
     4801 caaaatatgc cctgactgtc ggtgttggta cactgctgga tgccgaagaa gtgatgatta
     4861 tggtgctggg tagccagaaa gcactggcgc tgcaggccgc cgttgaaggt tgcgtgaacc
     4921 atatgtggac catcagctgt ctgcaactgc atccgaaagc gatcatggtg tgcgatgaac
     4981 cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta gaagcagaaa
     5041 atatcaaagg tctgtaattg ttatccctgc cctggttcct tgctcagggc aatatttttt
     5101 aaaatcgggg ggcagaatgt atgcattaac ccagggccgg atctttaccg gccacgaatt
     5161 tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct gtccggtagc
     5221 ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct cccccggttt
     5281 tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg ctgaagcggt
     5341 cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct gtactaacta
     5401 tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc gcgttatgcg
     5461 tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag gtccgtggct
     5521 gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg atgccgcgct
     5581 ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg caccggaaat
     5641 ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt ctgccggtca
     5701 ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc gcggggatta cctttgccac
     5761 ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg cgggcgcgat
     5821 cctcgacgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg ttgattacgc
     5881 caacattcgt aacgctaaac gccttaaagg cgacaaactg tgtctggtta ccgacgccac
     5941 cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa tatactaccg
     6001 taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa ccatgattga
     6061 aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc tgcgtatggc
     6121 gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt ctcggcacgc tcgccgcagg
     6181 taaagtagcc aacctgaccg cattcacacc tgattttaaa atcaccaaga ccatcgttaa
     6241 cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca agctcagata
     6301 ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct gattgaccag
     6361 tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc cgccagcgta
     6421 accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga tcagcaggcc
     6481 tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt ccacgcaatc
     6541 ggcgtacggc ttggtcgtca tgacgccacc atcactctgt ttgatctcag cagcaaagtg
     6601 ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga acatgccctg
     6661 ctgaatgcca ttgctcagtt tattgatagc taccagcgca agctgcgcga gctaatcgcc
     6721 atttcggtga tcctgccagg gcttgttgac ccggacagcg gcaaaattca ttacatgccg
     6781 catattcagg tagaaaactg ggggctggta gaagcactgg aagaacgttt taaagtgacc
     6841 tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt cggtgcaagt
     6901 caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc cgggattatc
     6961 tctaacgggc gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg ccatattcag
     7021 gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga aactatcgcc
     7081 gccaacgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta ccagagccgt
     7141 gtgccactgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa aggcgatagc
     7201 ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg gtaaaaccat cgccattgct
     7261 atcaacttat ttaatccgca aaaaattgtt attgctggtg aaatcaccga agccgataaa
     7321 gtgctgctcc ctgctattga aagctgcatt aatacccaag cgctgaaggc gtttcgtact
     7381 aatctgccgg tggtacgttc tgagctggat caccgctcgg caatcggcgc ttttgcgctg
     7441 gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa ttaatgtgct
     7501 tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt aaaaatgtaa
     7561 tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt gcagcggaat
     7621 ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac tatccttcgc
     7681 agactgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgtg ccggatagcg
     7741 tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgctaggaa ggcaagaaag
     7801 cgtatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt ttcactatta
     7861 ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg ttcctacaac tgggacatga
     7921 ttcataaagc agcctatttc gtcgctaacg gtgcacgctt tatcgccacc aacccggaca
     7981 cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt gagaaaatct
     8041 ccgggcgcaa accgttctat gttggtaagc ccagcccgtg gatcatccgc gcggcattaa
     8101 acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg cgataacctg cgtaccgata
     8161 ttctggcggg cttccaggcc ggtctggaga cgattctggt gctttctggt gtttcatcgc
     8221 tcgacgatat cgacagtatg ccgttccgcc caagctggat ttacccgtca gtcgctgaaa
     8281 tcgacgttat ctgaatacca cccacgtctg cggacgtggt tggctctgat gacaaacgcc
     8341 aaactgcctg atgcgctacg cttatcaggc ctacgcagct cctgcaatat attgaatttt
     8401 catgcttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca aatcgcactt
     8461 tatcagcaat ttggcccccg taaaatataa ccattcaata aaacccgcta aaaattatcg
     8521 attcatcaat aaatacatcc tcacaccaga ccctttttca ccaatcgaca atcaccatta
     8581 cgttttttat ttttgcactg ccaaatagct tgcgcacagt gctgctttgc ggcatttttt
     8641 taaacaagca aacacaacaa gcaacaaata ccaggttaac ggagaaggtt atgtgttcaa
     8701 tttttggcgt attcgatatc aaaacagacg cagttgagct gcgtaagaaa gcactcgagc
     8761 tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg tatttatgcc agcgataacg
     8821 ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg caacctctct
     8881 acaaccaaca aaaaaaccac gtactggcgg taaacggtga aatctacaac caccaggcat
     8941 tgcgcgctga atatggcgat cgttatcagt tccagacagg gtctgactgt gaagtgatcc
     9001 tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga cttgcagggc atgtttgcct
     9061 ttgccctgta cgacagcgaa aaagatgcct acctgattgg tcgcgaccat ctggggatca
     9121 tcccactgta tatggggtat gacgaacacg gtcagctgta tgtggcctca gaaatgaaat
     9181 cgctggtgcc agtttgccgc acgattaaag agttcccggc ggggagctat ttgtggagtc
     9241 aggacggcga aatccgttct tattatcatc gcgactggtt cgactttgat gcagtgaaag
     9301 ataacgtgac cgacaaaaac gagctgcgtc aggcactgga agattccgtt aaaagccatc
     9361 tgatgtctga tgtgccttac ggtgtgctac tttctggtgg tctggattcc tcaattattt
     9421 ccgctatcac caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc tctgaagcct
     9481 ggtggccgca gttacactcc tttgctgtag gtctgccggg ttcaccggat cttaaagcag
     9541 cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga aattcacttc actgtacagg
     9601 aaggtctgga tgccatccgc gacgtgattt accacatcga aacttatgat gtgaccacta
     9661 ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat caaggcgatg ggcattaaaa
     9721 tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg ttatctttac ttccataaag
     9781 cgcccaacgt taaagaactg catgaagaga cggtgcgtaa actgctggcc ttgcatatgt
     9841 atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg cgtggaagca cgcgttccgt
     9901 tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa cccgcaggat aaaatgtgcg
     9961 gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt tgagtcatac ctgcccgcaa
    10021 gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt cggttacagt tggatcgaca
    10081 ccctgaaaga agtggcggcg cagcaggttt ctgatcagca actggaaact gcccgcttcc
    10141 gcttcccgta caacacgccg acctcaaaag aagcgtatct gtaccgcgag atctttgaag
    10201 aactgttccc acttccgagt gccgctgagt gcgtgccggg cggtccttcc gtcgcgtgtt
    10261 cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa aatggacgat ccgtctggtc
    10321 gtgcggttgg cgttcaccag tcggcgtata agtaagaaat gcggtactgc cccggagaaa
    10381 tccggggctg ttttagttat tgcgctgctt gttgattctg atatgcagta aggaaatgtt
    10441 ccgggtttgc cacatcactg tttgacaact catcactgta ggccagtatt tcatagatta
    10501 atttcccacc actattgttg aattccgaat ttcatcctgt acgacaaaag aatggctgta
    10561 gggtggggaa aaacacaaag taaacaataa ttgacgaata tagcgccacg ctgttcgcaa
    10621 cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa agaggttgac gctgcaaggc
    10681 tctatacgca taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa gatggctacg
    10741 tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc gaatcccgtc
    10801 gtagccacca tcttttttgc gggagtggcg aaattggtag acgcaccaga tttaggttct
    10861 ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca gaaagcgttg
    10921 tacggatggg gtatcgccaa gcggtaaggc accggttttt gataccggca ttccctggtt
    10981 cgaatccagg taccccagcc atcttcttcg agtaagcggt tcaccgcccg gttattgggg
    11041 tatcgccaag cggtaaggca ccggtttttg ataccggcat tccctggttc gaatccaggt
    11101 accccagcca tcgaagaaat aatctggcta cgtagctcag ttggttagag cacatcactc
    11161 ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac caaattctga atgtgtcgaa
    11221 tacgttcggc aaattcaaaa
//
LOCUS       DAJTUP010000116        11188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTUP010000116 DAJTUP010000000
VERSION     DAJTUP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.116"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..613)
                     /gene="hdhA"
                     /locus_tag="OO468_003327"
     CDS             complement(<1..613)
                     /gene="hdhA"
                     /locus_tag="OO468_003327"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR6260321.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITPE"
     gene            complement(725..1753)
                     /gene="malI"
                     /locus_tag="OO468_003328"
     CDS             complement(725..1753)
                     /gene="malI"
                     /locus_tag="OO468_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR6260322.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1928..3520
                     /gene="malX"
                     /locus_tag="OO468_003329"
     CDS             1928..3520
                     /gene="malX"
                     /locus_tag="OO468_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR6260323.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3530..4702
                     /gene="malY"
                     /locus_tag="OO468_003330"
     CDS             3530..4702
                     /gene="malY"
                     /locus_tag="OO468_003330"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR6260324.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4806..5807
                     /gene="add"
                     /locus_tag="OO468_003331"
     CDS             4806..5807
                     /gene="add"
                     /locus_tag="OO468_003331"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR6260325.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5843..6883)
                     /locus_tag="OO468_003332"
     CDS             complement(5843..6883)
                     /locus_tag="OO468_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR6260326.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7126..7251
                     /gene="blr"
                     /locus_tag="OO468_003333"
     CDS             7126..7251
                     /gene="blr"
                     /locus_tag="OO468_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR6260327.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7524..7739
                     /gene="ydgT"
                     /locus_tag="OO468_003334"
     CDS             7524..7739
                     /gene="ydgT"
                     /locus_tag="OO468_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR6260328.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7825..8265
                     /locus_tag="OO468_003335"
     CDS             7825..8265
                     /locus_tag="OO468_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR6260329.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8342..8923
                     /gene="rsxA"
                     /locus_tag="OO468_003336"
     CDS             8342..8923
                     /gene="rsxA"
                     /locus_tag="OO468_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR6260330.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8923..9501
                     /gene="rsxB"
                     /locus_tag="OO468_003337"
     CDS             8923..9501
                     /gene="rsxB"
                     /locus_tag="OO468_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR6260331.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9494..>11188
                     /gene="rsxC"
                     /locus_tag="OO468_003338"
     CDS             9494..>11188
                     /gene="rsxC"
                     /locus_tag="OO468_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR6260332.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2598 a         2878 c         2817 g         2895 t
ORIGIN      
        1 tttctggtgt aataacggat ttcagggcat cggttaatat tgcccccggc gcaatgctat
       61 tcacccgaat atttttttca cccaggtcaa atgccatatt tctgaccaga tgactggccg
      121 cagctttaga tgatgcatag gaagtcatgt ttatattttt attttctgcc gccatagaag
      181 tgatggtcag aataacgcca cctccatttt tttccatttc tggcgcaaca agttgtgaca
      241 gatggaaaaa agaaaacaca ttcagttcat aagcacggcg aaaatctgcc attggcatat
      301 caaacggttt aggaccaccg ccaccggcgt tgttaaccag gatatcaact ttacccagat
      361 tactgacggc aaagtctgcc agtgcagaga gttcctgttc ggaagtaata tcacaacgcc
      421 aggcaaatgc ctgaccatcc agttgttgaa tttcgtctac aacatggtta gctgcgtcgg
      481 cgttaatatc actgaccacc acagatgcgc cagctgtcgc gaatgtaatg gcgatttctt
      541 taccaatacc tgcacccgca cctgtgatga tggcgcattt tccgtcgagt ctcaggttgt
      601 cagaattaaa cacataaacc tcctgtgaac ttcagtaaaa ataaaaggca ccgcctgtgt
      661 gccacatttt ttatcattaa ccttgaggaa aggacactac caagtatagc agataaagag
      721 aggattattt cgccgcaatg agccgggcgg gaataataag attgcgtgaa tgcgtctctt
      781 catgggtgat tttttgcatc atgcgatccg caagtgtgat accaagttcc cgcgctggcg
      841 tgctggccca ggtaacaggt atatcatcaa gtgtggttgg tgtcgcatcg gtaaatgccg
      901 ccagcgaaac ctgttgctca aagtaacgat cgactccgct ttccccgctt tgccgccctg
      961 ctttcagcaa accaaaccat gcccccatcg caatagtttc gttatagcaa accacggcac
     1021 tgatggtcgg gttatgacgt aaaagcgccg tgatagcttc cgcggcttgc ttctggctgg
     1081 aagtgcactc caacacccaa tcgctgtgaa acggcaggcc aaattttagt agagttgcac
     1141 aatagccccc cacccgttct gcacgggtta atgaggaact ttgccctccc agccaggcga
     1201 tccgctgatg cccattgcga atgagatgct ccgtcaacaa ctgtgcagcc tgcatgttgt
     1261 ccgggcgaac cgtatcaaca tcatcaagat aactggcacg ggaagcgaaa atcaccggga
     1321 tagctttttc ttctgccatc cgccgcaggt catcgctgct tcctgcagcc ccggcaatta
     1381 ccacaccatc gacaccctga ttcagtaaca gtgaaaaccg ctgtgccagc tgctcgccgt
     1441 ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgtgc ttccagagct tccgtcaatc
     1501 cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg gacgatcaaa ccaatgacgc
     1561 cgctttgccc gccgcgcagc gccgacgcct ggcgattgcg cacaaatccc agctcttcaa
     1621 tggcggcgtt aacgcgttct cctgtggcgg tagagattcg ccctttgcca ttaagcacca
     1681 gcgaaacggt acttaccgac acgcccgcag ccagcgcaac atcatgaatg gttatttttt
     1741 tggcggtagc catgcagatg acctactccc tgattatgtg acagataaaa cgttttacct
     1801 tttatttatc ttatacccgc tattatcgtt gcgtaatgtg atttatgact cactaaaatt
     1861 tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc agattgttct ctacgaggag
     1921 tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg tgggagttct tccagcagtt
     1981 aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc tgcggcatta tgctcggcat
     2041 tggtagttct cttagcagcc atgatgtcat aaccctgatc ccggtcctgg gcaaccccgt
     2101 gttgcaggct atctttacct ggatgagtaa gattggctcg tttgctttta gtttcctgcc
     2161 tgtcatgttc tgtatcgcca tcccgctggg cctggcacgc gaaaataaag gcgtagcggc
     2221 attcgctggc ttcgtcggtt atgcggtaat gaacctcgcg gtaaacttct ggttgaccaa
     2281 taaaggcatt ctgccaacca cagatgccgc cgttctgaaa gccaataaca tccagagcat
     2341 tcttgggatc cagtcgatcg ataccgggat cctcggtgcg gtgatcgccg gtattatcgt
     2401 ctggatgctg catgagcgtt tccataatat ccgcctgccg gatgcgctgg cattcttcgg
     2461 cggtacgcgc ttcgtaccaa ttatctcctc gctggtgatg ggccttgtcg gcctggtgat
     2521 tccattagtc tggccgattt tcgccatggg tattagcggc ttgggccata tgataaacag
     2581 cgcgggtgat ttcggaccga tgctgtttgg taccggtgaa cgtctgctgt tgccgtttgg
     2641 tctgcatcac attctggtgg cattaattcg ctttaccgac gcaggcggca cgcaggaggt
     2701 ctgcggtcaa accgtcagcg gcgcactgac catcttccag gcgcaattga gttgcccgac
     2761 cactcacggt ttttctgaaa gcgtcacgcg tttcctttcg caaggtaaaa tgcctgcgtt
     2821 tctcggcggt ctaccaggtg cagcgttagc tatgtatcac tgcgcgcgcc cggaaaatcg
     2881 ccataaaatt aaaggtctgc tgatttctgg cctgatcgcc tgcgtcgttg gcggcactac
     2941 cgaaccgctg gaattcctgt tcctgttcgt agcgccagtt ctgtatgtca tccacgcgct
     3001 gttaaccggc ctcggcttca ccgtcatgtc tgtgctcggc gtcaccatcg gtaataccga
     3061 cggcaatatc atcgacttcg tggtgttcgg tattttgcat ggtctgtcaa ccaagtggta
     3121 catggtgcca gtggtggcgg caatctggtt tgtcgtttac tacgtcatct tccgtttcgc
     3181 tatcacccgc ttcaatctga aaaccccggg gcgcgatagc gaagttgcca gctcaatcga
     3241 aaaagccgtt gccggtgcgc cgggtaaatc aggttacaac gttcctgcaa tcctcgaagc
     3301 attaggcggt gccgacaata ttgtcagcct cgataactgc attacccgtc tgcgtttgtc
     3361 tgtggaagat atgtcgcttg ttaatgtgca ggcactgaag gacaatcggg caattggcgt
     3421 agtacaactt aatcaacata acctgcaggt tgttatcggg ccacaagttc agtcagtaaa
     3481 agatgaaatg gccggtctga tgcatactgt ccaggcataa ggataagata tgttcgattt
     3541 ttcaaaggtc gtggatcgtc atggcacatg gtgtacacag tgggattatg tcgctgaccg
     3601 tttcggcact gctgacctgt taccgttcac gatttcagac atggattttg ccactgcccc
     3661 ctgcattatc gaggcgctga atcagcgcct gatgcacggc gtatttggct acagccgctg
     3721 gaaaaacgat gagtttctcg cggctattgc ccactggttt tccacccagc attacaccgc
     3781 aattgatacg cagacggtgg tgtatggccc ttctgtcatc tatatggttt cagaactgat
     3841 tcgtcagtgg tctgaaacag gtgaaggcgt ggtgatccac acgcccgcct atgacgcatt
     3901 ttacaaggcc attgaaggta accagcgcac agtaatgccc gttgctttag agaagcaggc
     3961 tgacggctgg ttttgcgata tgggcaagct ggaagccgtt ttggcgaaac cagaatgtaa
     4021 aattatgctc ctgtgtaacc cacagaatcc taccgggaaa gtgtggacgt gcgatgagct
     4081 ggagatcatg gctgacctgt gcgagcgtca tgatgtgcgg gttatttccg atgaaatcca
     4141 tatggatatg gtctggggcg agcagccgca tattccctgg agtaatgtgg cgcggggaga
     4201 ctgggcgttg ctgacatcgg gatcgaaaag tttcaatatt cccgcgctga ccggtgctta
     4261 cgggattata gaaaatagca gcagccgtga tgcctattta tcggcactga aaggacgtga
     4321 tgggctttct tccccttcgg tactggcgtt aactgcccat atcgccgcct atcagcaagg
     4381 tgcaccgtgg ctggatgcct tacgcgtcta tctgaaagat aacctgacgt atatctcaga
     4441 taaaatgaac gccgcgtttc ctgaactcaa ctggcagatc ccgcaatcca cttatctggc
     4501 ctggcttgat ctacgtccgt tgaatattga cgacaacgcg ttgcaaaaag cgcttatcga
     4561 gcaagaaaaa gtcgcgatca tgccggggta tacctatggt gaagaaggtc gtggttttgt
     4621 ccgtctcaat gccggctgcc cacgttcgaa actggaaaaa ggtgtggctg gattaataaa
     4681 cgccatccgc gctgttcgct aaccccaatt gcgcaacgta aaaaatcgtt gcgcaatcgt
     4741 ggatttttac cctgctttgt ttttataatg gtgcgcactt ttatatccag aaaaagagtg
     4801 cgaccatgat tgataccacc ctgccattaa ctgatatcca tcgccacctt gatggcaaca
     4861 ttcgtcccca gaccattctt gaacttggcc gccagtataa tatctcgctt cctgcacaat
     4921 cccttgaaac actgattccc cacgttcagg tcattgccaa cgaacccgat ctggtgagct
     4981 ttctgaccaa acttgactgg ggcgttaaag ttctcgcctc tcttgatgcc tgccgccgcg
     5041 tggcatttga aaacattgaa gatgcagccc gtaacggcct gcgctatgtc gagctgcgtt
     5101 tttcaccagg ctacatggca atggcacata agctgcctgt agcgggtgtt gtcgaagcgg
     5161 tgatcgatgg cgtacgtgaa ggttgccgca cctttggtgt gcaggcgaag cttatcggca
     5221 ttatgagccg aaccttcggc gaagccgcct gtcagcaaga gctggaggcc tttttagccc
     5281 accgtgacca gattaccgca cttgatttag ctggtgatga acttggattc ccgggaagtc
     5341 tgttcctttc tcacttcaac cgcgcgcgtg atgcgggctg gcatattacc gtccatgcag
     5401 gcgaagctgc cgggccggaa agcatctggc aggcgattcg tgaactgggt gcggagcgta
     5461 ttggacatgg cgtaaaagcc attgaagatc gggcgctgat ggattttctc gccgagcaac
     5521 aaattggtat tgaatcctgt ctgacctcca atattcagac cagcaccgta gcagagctgg
     5581 ctgcacatcc gctgaaaacg ttccttgagc atggcattcg tgccagcatt aacactgacg
     5641 atcccggcgt acagggagtg gatatcattc acgaatatac cgttgccgcg ccagctgctg
     5701 ggttatcccg tgagcaaatc cgccaggcac agattaatgg tctggaaatg gctttcctca
     5761 gcgcagagga aaaacgcgca ctgcgagaaa aagtcgccgc gaagtaacaa aaatggatgg
     5821 tgcaaattgc accatccatt tttcacgcaa ggcacaacgt cgcgcgatgt ttggcggatt
     5881 cgatgcccag ctcaatcaac tccattacct ggattgcctg gcttgccgga accggatttt
     5941 caccatcgcc atttaacgca tcacgaatcg ccgcatagta agccggataa ttcccaggca
     6001 ccgtcaacag cgtttcttcg acacgttcct caccttccac gcgggtaagt acgccatcac
     6061 gcatatcgta gccccagtct tcctgcggca gacgctcgcc atttttcaga cgttcttcct
     6121 gtggatcgag gccatatttc acataactgc ctcgggatcc atgcacgata taccgtgctg
     6181 actcagcggc tgccagcatg gtaccgtgta aaatgactcg ccgctgtgga taggacaaga
     6241 tggcgtggaa ataatcggtc gactgcgctc cgggccgtaa ctgtgccaaa tctaccgtca
     6301 tgctgaccgg taaaccaaat agcgtaatgg cctgatcaag aagatgcggt gctaaatcgt
     6361 accagatacc gctgcctggg ccgccctgtt cacgccaacg atcgcgcacc tgcggacgga
     6421 agcggtcaaa atgagactca aagtaagcaa cttcacccag cacgccttcc gcgagcaaac
     6481 cttttagcgt caagaaatcg ctatcccaac ggcggttatg gaatacagac agcacacgcc
     6541 ccaggctttt tgccagcgca tccagctctc gcgcttgtga cagtgtcacg gtaaagggtt
     6601 tatcaacgac cacatgttta cccgcctcaa gcgccgcttt ggctaacggg aaatgggtat
     6661 cgttgggtgt aggaatgaca atcaggtcta tgttgggatc gttaaacaga tgcttcggct
     6721 cagagacaac cgtaaccgtt ggccagtcgg cttttacttt tgtttcatca ctgctggaga
     6781 ttaccgccag ttccagcccg ggcgtgcccg caatcagggg cgcatggaag gttttgctcg
     6841 cataaccata cccaatcaac ccaacacgga tgttgtcgct catgtcattg cctctcattg
     6901 aagtatgatg gctatttgac actatccttt acccacgctc aacagtttaa taacctgcca
     6961 gcaataaggg atgttgttta acttaagtca aaaaaatagc gaattttcca acgacaaaag
     7021 ctaaatatcg caaaaacctc agtaaaaatc ttgctggagc tattattgct aagtaacatt
     7081 taccccctga agttaatgga tcaatcaaga gagatgtggg ctgtaatgaa tcgtcttatt
     7141 gaattaacag gttggatcgt tcttgtcgtt tcagtcattc ttcttggcgt ggcgagtcac
     7201 attgacaact atcagccacc tgaacagagt gcttcggtac aacacaagta agctctgcac
     7261 ttgtggagcg acatgctgcc cgtccgggtg catgttttca cttgtcggat attaaaccag
     7321 gaatttatta tcttgttcga tgttgttggt gattgtcagg gatagtaaag cgcgttactc
     7381 ttttcagagc agtttttcat ccctgaatat ttgccatcag cgcattctta ttattgtaat
     7441 aacacaagcg ttcacttttg gtgaaatttt atcatcacta ataataccta cccctttggt
     7501 attaattatt ttatggacct tttatgactg ttcaggacta cttattaaaa tttcgcaaaa
     7561 tcagttcact cgaaagtctg gaaaaactct acgaccatct taattacacc ctgacggacg
     7621 atcaggaact gatcaatatg tatcgtgctg ccgatcaccg tcgcgcagag ctggtttctg
     7681 gcgggcgttt gtttgacctc ggccaggtac cgaagtccgt ctggcactat gtccaataaa
     7741 gaaagtagcg atcggttata aaagccttat aataacgccc ctaaaattag atttgcctgt
     7801 ttgcgtcgct attctggaga acctatgacc accacaacgc cacaacgtat tggaggctgg
     7861 ttgcttggcc cgttggcctg gcttttagtc gctctgttga gtacgacgct ggcgttgttg
     7921 ctgtataccg ctgcgttatc ttctccgcaa acatttcaaa cgctcggcgg acaagcactg
     7981 acgacacaaa tcttatgggg cgtttctttt attaccgcta tcgccatgtg gtattacaca
     8041 ctgtggttga ccatcgcttt ctttaaacgt cggcgttgtg ttcctaaaca ctatattatc
     8101 tggctgctta tttccgtatt gctggcagta aaagcattcg ctttttcacc cgtggaagtt
     8161 ggcatagccg ttcgccagtt gctgtttact ttgttggcaa ccgcattgat cgtcccctat
     8221 ttcaaacgct cgtcgcgggt aaaagcgacg tttgtgaatc cgtaataacc ttacagttaa
     8281 cctgttgtcg cctgctctgg attaacggat aataggcggc ttttttattt caggccgaaa
     8341 aatgactgac tacctgttac tgtttgtcgg aactgtactg gtcaataact ttgtactggt
     8401 caagtttctc ggtctctgtc cgtttatggg ggtttccaaa aaactggaaa ccgcgatggg
     8461 catggggctg gcaacaacgt ttgtgatgac gctgacgtct atttgcgcct ggcttatcga
     8521 tacgtggatt ttgatcccac ttaatctgat ttacctgcgc accctggcat ttattctggt
     8581 gattgctgtg gtcgtgcagt tcaccgagat ggtggtgcgc aaaaccagcc cggtgcttta
     8641 ccgtctgctg gggatttttt tgccgcttat caccaccaac tgtgcagtgc tcggcgtggc
     8701 gttgctgaat atcaatctcg ggcacaattt cttgcagtcg gcgctgtacg gtttttccgc
     8761 cgctgtcggt ttctcgctgg tgatggtgct cttcgccgcc atccgcgaac gccttgctgt
     8821 ggctgatgtc ccggcacctt ttcgcggtaa tgccattgcg ttaattaccg caggtcttat
     8881 gtctctggcc tttatgggct ttagtggttt ggtgaagttg taatgaatgc tatctggatt
     8941 gccgttgccg ccgtgagcct gctgggcctg gcgtttggcg ccattctggg ttatgcctcc
     9001 cgccgttttg cggtggaaga cgatccggtc gttgagaaaa ttgacgaaat cttaccgcag
     9061 agccagtgtg gtcagtgcgg ttatcccggc tgtcgcccct acgcggaagc catcagctgt
     9121 aacggtgaaa aaatcaaccg ttgcgcccca ggtggcgaag ctgtgatgct aaaaattgcc
     9181 gagttgctta atgtcgagcc gcagccgctg gatggcgaag cgcaagagct aacgcctgcg
     9241 cggatggtgg cggttattga tgaaaataac tgtattggct gcactaaatg tattcaggct
     9301 tgtccggtag acgccatcgt tggcgctacc cgtgtcatgc atacggtaat gagtgatctc
     9361 tgtacgggct gcaatttatg tgttgatccg tgcccgacgc actgcatctc gttgcaaccg
     9421 gtcgcagaaa cacctgactc ctggaaatgg gatctgaaca ccattcccgt gcgtatcatt
     9481 cccgtggaac accatgctta agttattctc tgcattcaga aaaaataaaa tctgggattt
     9541 caacggcggc atccatccac cggagatgaa aacccagacc aacggtaccc ccctgcgcca
     9601 ggtactactg gcgcagcatt ttgttattcc actgaaacag catattggcg ctgaaggtga
     9661 gttgtgcgtt agcgtcggcg ataaagtatt gcgcggccag ccgcttaccc gtggtcgcgg
     9721 caaaatgctg cctgttcacg cgcccacctc gggtaccgtt acggctattg cgccccactc
     9781 tacggctcat ccttcagctt tagctgaatt aagcgtgatt attgatgccg atggtgaaga
     9841 ctgctggatc ccgcgcgacg gctgggtcga ttatcgctct cgcagtcgcg aagagttaat
     9901 cgagcgcata catcagtttg gtgttgccgg gctgggcggt gccggattcc cgacaggcgt
     9961 taaattgcag ggtggtggag ataagattga aacgttgatt atcaacgctg ctgagtgcga
    10021 gccgtacatt accgccgatg accgtttgat gcaggattgc gcggctcagg tcgtagaggg
    10081 tattcgcatt cttgcgcata ttctgcagcc acgcgaaatt cttatcggca ttgaagataa
    10141 caaaccgcag gcgatttcca tgctgcgcgc ggtgctggcg gactctaacg atatttctct
    10201 gcgggtgatt ccaaccaaat atccttctgg cggtgctaaa caattaacct acattctgac
    10261 cgggaagcag gttccacatg gcgggcgttc atccgatatc ggcgtattaa tgcaaaacgt
    10321 cggcactgct tatgcagtga aacgtgccgt tattgatggc gagccgatta ccgagcgtgt
    10381 tgtaaccctg actggcgaag caatcgctcg cccgggcaac gtctgggcac ggctggggac
    10441 gccagtgcgt catttattga atgatgccgg attctgcccc tctgccgatc aaatggtgat
    10501 tatgggtggc ccgctaatgg gctttacctt gccatggctg gatgtcccgg tcgtaaagat
    10561 taccaactgt ctgttggctc cctctgccaa tgaacttggc gaaccacagg aagaacaaag
    10621 ctgcatccgg tgtagcgcct gtgctgacgc ctgccctgtg gatcttttgc cgcaacagtt
    10681 gtactggttc agcaaaggtc agcaacacga taaagctacc acgcataaca ttgccgactg
    10741 cattgaatgt ggggcttgcg cgtgggtttg cccgagcaat attcccttgg tgcaatattt
    10801 ccgtcaggaa aaagctgaaa ttgcggctat tcgtcaggaa gaaaagcgcg ccgcagaagc
    10861 caaagcgcgt ttcgaagcgc gccaggctcg tctggagcgc gaaaaagcga ctcgccttga
    10921 acgacataag agcgcagccg ttcaacctgc agccaaagat aaagatgcga ttgctgccgc
    10981 tctggcgcgg gtgaaagaga aacaggccca ggctacacag cctattgtga ttaaagcggg
    11041 cgaacgcccg gataacagtg caattattgc agaacgggaa gcccgtaaag cgcaagccag
    11101 agcgaaacag gcagaactgg agcaaactaa cgacgcagca accgttgctg aaccacgtaa
    11161 aactgccgtt gaagcagcta tcgcccgc
//
LOCUS       DAJTUP010000117        10918 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTUP010000117 DAJTUP010000000
VERSION     DAJTUP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.117"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            220..1860
                     /gene="trg"
                     /locus_tag="OO468_003339"
     CDS             220..1860
                     /gene="trg"
                     /locus_tag="OO468_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR6260333.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1898..2821)
                     /locus_tag="OO468_003340"
     CDS             complement(1898..2821)
                     /locus_tag="OO468_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6260334.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3038..4381
                     /locus_tag="OO468_003341"
     CDS             3038..4381
                     /locus_tag="OO468_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6260335.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4606..6261
                     /gene="opgD"
                     /locus_tag="OO468_003342"
                     /gene_synonym="mdoD"
     CDS             4606..6261
                     /gene="opgD"
                     /locus_tag="OO468_003342"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR6260336.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6401..6625
                     /locus_tag="OO468_003343"
     CDS             6401..6625
                     /locus_tag="OO468_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR6260337.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6688..7224
                     /gene="rimL"
                     /locus_tag="OO468_003344"
     CDS             6688..7224
                     /gene="rimL"
                     /locus_tag="OO468_003344"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR6260338.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7219..8199)
                     /locus_tag="OO468_003345"
     CDS             complement(7219..8199)
                     /locus_tag="OO468_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260339.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8323..9315
                     /gene="tehA"
                     /locus_tag="OO468_003346"
     CDS             8323..9315
                     /gene="tehA"
                     /locus_tag="OO468_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR6260340.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9312..9905
                     /gene="tehB"
                     /locus_tag="OO468_003347"
     CDS             9312..9905
                     /gene="tehB"
                     /locus_tag="OO468_003347"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR6260341.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10208..10864
                     /locus_tag="OO468_003348"
     CDS             10208..10864
                     /locus_tag="OO468_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR6260342.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
BASE COUNT         2824 a         2551 c         2745 g         2798 t
ORIGIN      
        1 aataatcact tcacgaaatt gcgtgctgtt ttccagaatt tttcgtcatt cgggttagcc
       61 agtttagcca ttcgttactc tcttcattcc aatagcatta attttctctg caataattgt
      121 tgtaaaaatg tgacgcaaag aggtttttgg tcataagtaa ttaccgtcaa gtgccgatga
      181 ctttctatca ggagtaaacc tggacgagag acaacggtaa tgaatacaac tccctcacag
      241 cgattaggtt ttttgcatca catcaggctg gttccgttat ttgcctgcat tcttggcggt
      301 atcttagttc tattcgcatt aagttctgct ctggctggct atttcctctg gcaggccgat
      361 cgcgatcagc gtgatgttac tgcggagatt gagattcgga ccgggttagc gaacagttca
      421 gattttttgc gttcagcccg gatcaatatg attcaggccg gggctgcgag tcgtattgcg
      481 gaaatggaag caatgaagcg aaatattgcg caagccgaat cggagattaa acagtcgcag
      541 caaggttatc gtgcttatca gaatcgaccg gtgaaaacac ctgctgatga agccttcgac
      601 actgaattaa atcaacgctt tcaggcttat atcacgggta tgcaacctat gttgaaatat
      661 gccaaaaatg gcatgtttga agcgattatc aatcatgaaa gtgagcagat ccgaccgctg
      721 gataatgctt ataccgatat tttgaacaaa gccgttaaga tacgtagcac cagagccaac
      781 caactggcgg aactggccca tcagcgcacc cgcctgggtg ggatgttcat gattggcgcg
      841 tttgtgcttg ccctggtcat gacgctgata acatttatgg tgctacgtcg gatcgtcatt
      901 cgtccactgc aacatgccgc acaacggatt gaaaaaatcg ccagtggcga tctgacgatg
      961 aatgatgaac cggcgggtcg taatgaaatc ggtcgcttaa gtcgtcattt acagcaaatg
     1021 cagcattcac tggggatgac agtagggact gttcgacagg gcgcggaaga gatttatcgt
     1081 ggcaccagcg aaatttcagc tggcaatgcg gacctgtcat ctcgcaccga agaacaagcg
     1141 gcggctatcg aacaaactgc cgccagcatg gagcaactca ctgcgacggt gaaacagaat
     1201 gcggataacg cgcatcatgc cagcaaactg gcgcaagagg cttctattaa agccagcgat
     1261 ggcgggcaga cggtttccgg tgtagtaaaa acgatgggcg ctatctccac gagttcgaag
     1321 aaaatttctg agatcaccgc cgtcatcaac agtattgctt tccagacgaa tattctggca
     1381 ctgaatgctg ccgttgaagc cgcgcgagcg ggtgagcaag ggcgtggatt tgccgttgtc
     1441 gccagcgaag tacggacact cgcaagccgc agcgctcagg cagcgaaaga gattgaaggc
     1501 ttgatcagtg aatcagtcag gttaattgac ctggggtcgg atgaggtggc aacggcaggg
     1561 aaaaccatga gcactattgt tgatgccgtc gcgagtgtca cacatatcat gcaggaaatc
     1621 gccgccgcct cggatgaaca aagtagaggc ataactcagg ttagccaggc gatttctgaa
     1681 atggataagg tgacgcaaca gaatgcttct ctggtagaag aggcctcagc agcggcggtg
     1741 tcccttgaag aacaggctgc acgattaact gaggcggtga acgtattccg tctgaacaaa
     1801 cattctgtgt cggcagaacc tcgcggagcg ggtgaaccag ttagtttcgc tacggtgtga
     1861 aaatgttcaa ggagggatcg acagatccct tcacctttca gaacggcatt gattttcgaa
     1921 tagcgttaat catcaactgg caaccagaag agaacgtcgc atctacgcgg gtcagtattc
     1981 caatcggttc gcctgcacca tgtcccggaa caggcagggc caccagcgtg gcatgacgca
     2041 ggtcgtcttt tacagcgcca gaagggacaa accacacgta atcgtattca acggtaagtt
     2101 gacgagatag cgaagtagac agcgtttcga tacaacccga aggaatttta cagccctggc
     2161 tctgcactaa tgcatctgaa tgctggcgtg gcgcagtgcc ttctggtgat acaacgaccg
     2221 gccattccag cacccggctt agcgttacgt tctcctgaag tagcgggtga ttagggcgga
     2281 caaccagctt caacgattca agaaacagca gttcgtaatt aagcccggtc atcagttcag
     2341 gatctgacat ccgaccaatg ccgatatcga tttccccggt ttttaaaccc gccagaatca
     2401 tagggttact cattgtcgca acttgcaagg tcgtctcttt ttgttgctga tgaaactgac
     2461 ctataaccga aggtaatatc cccagtgccg cagtaggtag tgcaccaacc ctgacgacat
     2521 cattattaag accttcttta cgatgaagcg actgtccggc agtgttgatg gcgtcaagaa
     2581 ctctgactgc atgcgttaaa aattgttcgc cgggtaaggt aagttgcgcc ccctgacgac
     2641 cacgctcaaa caagcgagcg cccgtcagct gctccagttc attcaatgta ttagagagcg
     2701 caggttgact caaattaagg gtttcagctg cgcgccccaa agttccttgt tgtgcgacag
     2761 ctacgaatgt atgaaggtgg cgcaaacgga tgcgctgact aaacagacta tttttttcca
     2821 taagcgatgt taaaaacgaa gcggtgtcgc tgacaagtga agttgtttga ttatgataac
     2881 ttgattgcaa aatattatta acaattaaag caattatgtt acagcaaaat ggataatatt
     2941 gatgttttcg cggcgagatc acagtttgta aattcttccc gccagagtga atgcggttac
     3001 ctacactcca gattactgac cactggaggc agacactatg gcgaacagca tcacggcgga
     3061 tgagattcgg gaacagtttt cgcaggcaat gtcagccatg taccagcaag aagttccgca
     3121 atatggcacg ctgctggaac tggtagctga tgtgaatctg gctgtgctgg aaaacaatcc
     3181 tcaactgcac gaaaaaatgg taaatgcaga cgagctggcg cgactgaatg ttgaacgtca
     3241 tggggcgatt cgcgttggga ctgcacaaga gcttgctact cttcggcgga tgtttgccat
     3301 tatggggatg tacccggtga gctattacga tctctcgcag gcaggggtgc cggtacattc
     3361 gacagcattt cggcccattg atgatgcttc tctggcgcgt aatcccttcc gcgtttttac
     3421 atccttactc cgccttgagc ttatcgagaa cgaaattttg cgccagaaag cggcggagat
     3481 tctgcgtcag cgcgatatct tcaccccacg ttgtcgacaa ctgttagagg aatatgagca
     3541 gcagggcggt tttaacgaaa cacaggcaca ggagtttgtg caggaagccc tggaaacgtt
     3601 tcgctggcac cagttagcaa cggtagatga agaaacctat cgcgccttgc acaacgaaca
     3661 tcggttgatt gctgatgtgg tctgttttcc tggatgccat atcaaccacc tgacgccacg
     3721 tacgctggat attgaccggg tgcagtcgat gatgcctgaa tgcggaattg aacccaaaat
     3781 tctcatcgag gggccgccgc gccgcgaggt accgatttta ctacgccaga ccagctttaa
     3841 agcactggaa gagacggtgt tgtttgcggg gcagaaacag ggcacgcata ccgcgcgctt
     3901 tggtgaaatt gagcagcgtg gcgtggcatt aacgccgaaa gggcgacaac tgtatgatga
     3961 tcttctgcgt aacgctggaa ccgggcagga taatctcact caccaaatgc atttacagga
     4021 aaccttccgc acttttcctg acagtgagtt tttaatgcgt cagcaagggt tggcatggtt
     4081 ccggtaccgt ctgacgcctt cgggtgaggc gcatcgtcag gcgattcatc ctggagacga
     4141 tccacagccc ttaattgaac gtggttgggt agtggcgcaa cccatcacct atgaagattt
     4201 cttgcccgtt agcgcggcgg ggatcttcca gtcaaatctg ggtaatgaaa cgcagacacg
     4261 cagtcacggt aatgccagtc gcgaagcatt tgagcaggcg ttgggttgtc cggttttgga
     4321 tgagttccag ctttaccagg aagcggaaga acgcagtaaa cgtcgctgtg gtttgcttta
     4381 aattctgacc atccgccttt gcaaaaattt gcctgatttt acaaacgaat caggctcatc
     4441 ccatcgacat aaaaaaaatg ccgatttatg catattctct cagttcaaca attggattat
     4501 taataaatat tgtctagagt gagcggtcat aaataagcac tttcttgccg ctgaaaacga
     4561 ccagcgcggg accattcaca acaccagaag gactcacttt caggtatgga tcgtagacga
     4621 tttattaaag gttcaatggc tatggccgcc gtgtgcggta ccagcggcat tgcttctctt
     4681 ttttctcagg cagcattcgc ggcagattct gatattgccg acgggcaaac ccagcgtttt
     4741 gacttctcca ttctacagtc aatggcgcac gacttagcgc aaacagcgtg gcgtggtgcg
     4801 cctcgtccgt tacctgacac gctggcgaca atgacgccgc aggcttataa cagtattcaa
     4861 tacgacgccg aaaaatcgct ctggcataac gttgagaacc gtcaactgga cgctcagttc
     4921 ttccatatgg gaatgggatt ccgtcgccgc gttcgtatgt tttctgtaga tccagcaaca
     4981 catctggcgc gtgaaattca ctttcgcccg gagttgttca aatacaacga tgcaggtgtt
     5041 gataccaaac aattagaagg gcaaagcgat ctcggctttg ccggttttcg cgtgtttaaa
     5101 gcccccgaac tggcgcgccg tgatgtagta tcatttctcg gcgcgagtta tttccgcgcc
     5161 gttgatgata catatcaata cggtttgtcg gcccgcggcc tggcgatcga cacttacacc
     5221 gacagtaaag aagagttccc cgactttacc gccttctggt ttgatacggt aaaaccgggg
     5281 gcaactacct ttaccgttta tgcgttgctc gatagcgcca gcattactgg tgcctataag
     5341 ttcactatcc attgtgagaa aagtcaggtg attatggatg tggaaaatca cctgtatgcg
     5401 cgcaaagaca ttaaacagct gggcatttcg ccgatgacca gtatgttcag ctgcggtact
     5461 aatgaacgtc ggatgtgcga tacaattcat ccgcaaattc atgactctga tcgtctgtcc
     5521 atgtggcggg gcaacggcga gtggatttgc cgtccgctga ataatccgca aaaattgcag
     5581 ttcaatgctt acaccgacaa caacccgaaa gggtttggtt tattgcaact ggatcgtgac
     5641 ttctcccatt atcaggacat tatgggctgg tataacaaac gcccaagtct gtgggtggaa
     5701 ccgcgtaaca agtggggtaa gggcaccatc ggcctgatgg aaatcccaac aacgggcgaa
     5761 acgctggata acattgtctg cttctggcag ccagaaaaag ctgtaaaagc aggtgatgag
     5821 tttgcattcc agtatcgtct gtactggagt gcgcaaccgc ctgttcattg cccattagcg
     5881 cgcgttatgg cgacgcgtac cggcatgggc ggtttcccgg aaggttgggc gccaggtgaa
     5941 cactatcccg aaaaatgggc gcgtcgtttt gccgtcgatt tcgttggtgg tgatctgaaa
     6001 gctgccgcgc caaaaggcat tgagccggtg attacgcttt ccagtgggga agcgaagcaa
     6061 atcgaaattc tctatattga acccatcgat ggttatcgta ttcagtttga ctggtatccg
     6121 acttcggact ccactgatcc ggtcgatatg cggatgtatc tacgttgtca gggggacgct
     6181 atcagtgaaa catggctgta tcagtatttc ccgccagcgc cggataaacg tcagtatgtt
     6241 gacgaccgcg tgatgagtta atcgtttttt cttcggcacc ttcttcggga ggtgccgtct
     6301 ggttaaacac gatcccgctc gcatttttcc ctaagttaaa tgagtaatct gatggtgtgt
     6361 atttcagata caccgtgtca gccactaaca gggagtgcgt atgtttccag aataccgaga
     6421 tttaatatcc cgtctgaaaa acgaaaatcc tcgctttatg tccttgttcg ataaacacaa
     6481 taaacttgat catgaaattg ccagaaagga aggttccgac ggtcgagggt acaatgcgga
     6541 agtggtccgc atgaaaaaac aaaagctaca gttaaaagat gagatgctca aaatcctgca
     6601 gcaggagagc gtcaaagagg tgtaaacttt cctaagccgc cagccaggcg gcttttttaa
     6661 caacagcatg gattgactgg agataagatg actgaaacga taaaagtaag cgaatcactt
     6721 gaattacatg ctgttgcgga aagtcacgtc acacctcttt atcagttaat ctgtaaaaat
     6781 aaaacctggt tacagcagtc gctaaactgg ccgcagttcg ttcagagtga agaggacacg
     6841 cgaaaaacgg tgcagggtaa tgtgatgttg catcaacgcg gctatgccaa aatgttcatg
     6901 attttcaaag aagatgaact tatcggcgtt atctcgttta atcgtattga accactgaat
     6961 aaaactgctg aaatcggcta ctggctggac gaatctcatc aggggcaggg gatcatttct
     7021 caggcgctgc agacattgat tcatcattac gctcagtctg gtgaacttag acgcttcgtg
     7081 atcaaatgtc gggtggacaa tccgcaaagc aaccaggtcg ctttgcgcaa tggttttatc
     7141 cttgaaggtt gcctgaaaca ggctgagttc ctgaatgatg cctatgatga tgtgaactta
     7201 tacgcgcgta ttatcgattc ataataaccc tgccagcggc gttcgcgtaa tgcgctcctc
     7261 gccgttaatg acttttggac cacggacatg aaccgtgtcg ccatcaaagg cttcaacgac
     7321 ggcgtggtcg gttaactcca catggttttc aattatcacc gcacctgtaa tacggctctc
     7381 accttgtatt acaacgtgct cgtcgagcag aatcggtcca ccacgtacca cggcatttcc
     7441 gccaatcaag acatgatgtt ttaacacaca attaccttcc acaatggcat attccgccac
     7501 ctgcgaactg taatgaatcg tcggaatggc atcttcttct atgccagctt tcacctgcgc
     7561 gtgaccgtag actttagcgc aatcgcatag ccagacgttg ttctcttcat taccttcaac
     7621 actggcaaaa tcaaaaactt cggcgcgatg ttcaataaaa gcataccgga cgacggcatc
     7681 gccataaatt tgtgcctggt ggacaatacg cgaggcgcta acccttgcgc gatcataaat
     7741 ttggagcagg agttgatggt cgggcgttaa gccttgtgcg gcgacgatca tagaatgttg
     7801 atcaattaag gcgtgaccaa atattctaca ttgcacgtaa actaatgagt catgaatggt
     7861 gacactgtca cttatatatg caccctggct gatttcacta ttatcgatcc agacgttatc
     7921 cgttgcgtaa acctctcccc acagcacgct ggtgccggtg atacgcgtat taccggaaat
     7981 caccgttccg ccgaacgcaa tagcattctg gtcgtaaatc cagcaatttc cttcctgtgc
     8041 taacaccgtt tcgcgatcga tccagccgcc agcggttcct gctatcacat cgttaaaatc
     8101 gctaatggcg ataatctgcc gtaataacac acttttttta gtgccatctt cctgataact
     8161 aaaggctcgc tgttcttcac ttaggcgata tttgcgcata gcgttttccc acaggtgact
     8221 tactataacc gtagcaaatt ctgcggctct ggctatgctc gagaaaattc cataaaatgc
     8281 atttcaaata tactttataa attaaacaaa atgagtaaga agatgcagag cgataaagtg
     8341 ctcaatttgc cggcaggcta ctttggtatt gtgttgggga cgatagggat gggatttgcc
     8401 tggcgctatg ccagccaggt ttggcaggtc agccactggt taggggatgg gctggtgatt
     8461 ctggcgatga tcatctgggg attattgact agcgcattta ttacccgact catacgcttt
     8521 ccgcatagcg tgctggcgga agttcgccat ccagtgctga gcagttttgt gagtttgttt
     8581 ccggcaacga cgatgctggt ggcgattggt tttgttccgt ggtttcgccc actggcggtg
     8641 tgcatgttca gttttggtgt cgtggttcag ttggcttatg ccgcctggca aactgcggga
     8701 ttatggcgcg gatctcaccc tgaagaagct accacgcctg gactgtatct gccgacagtt
     8761 gccaacaact ttatcagcgc aatggcctgt ggtgcgttgg gctacaccga cgccggtctg
     8821 gtgtttttag gcgcaggcgt tttctcatgg ctaagccttg aaccggtgat cttgcagcgt
     8881 ctgcgcagtt cgggagaatt acccacggca ctgcggacat cactcggcat tcagctcgct
     8941 cctgcgctgg tggcctgtag tgcctggctg agcgtcaacg gcggcgaggg tgacacgctg
     9001 gcgaaaatgc ttttcggtta tggactgctg caactgctgt ttatgctacg tctgatgcca
     9061 tggtatctct cccagccatt taatgcttca ttctggagtt tctcgttcgg cgtatctgca
     9121 ctggcaacca ccggtttgca tctggggagt ggcagcgata atggattttt ccatacgctg
     9181 gcggtgccgc tgtttatctt taccaatttt attattgcaa tactgctcat ccgtactttt
     9241 gcgcttctga tgcagggaaa attgttagtc agaaccgagc gcgccgtttt aatgaaagca
     9301 gaggacaaag aatgatcatt cgtgacgaaa actattttac tgataaatat gaattaaccc
     9361 gcacacactc tgaagtactg gaagcggtga aagtggttaa accgggtaaa acgctggatc
     9421 tgggctgtgg caatggtcgt aacagtcttt acctggcagc caatggttat gatgttgacg
     9481 catgggataa aaatgccatg agtatcgcca acgtcgagcg cattaaatcc attgaaaatc
     9541 tggataattt acacacccgg gtcgttgatc tgaataacct cacatttgat ggacagtacg
     9601 attttattct ttcgactgtg gtgctgatgt tccttgaggc taaaaccatc ccagggttga
     9661 ttgccaatat gcaacgttgc actaaacctg gtggttacaa cctgattgtg gcggcgatgg
     9721 ataccgctga ttatccatgt accgtcggct tcacgtttgc cttcaaagag ggagaattac
     9781 gtcgatatta cgaaggctgg gagagggtga aatacaatga agacgtcggc gagctgcacc
     9841 gcaccgacgc caacggtaat cgtattaaac tgcgtttcgc cacgatgctg gcacgtaaaa
     9901 aatgacccgg taagcacaaa acgcgtgaaa attccccacg ctgagatgat ttactgttct
     9961 tcttttcggt aagcatattt tttatcgaag ggatgtgaaa ttaatcacag tagtcgaagt
    10021 tttttagcag cttaacttac tgaaatttaa ctactgatga ttgacttagc ccctttttcg
    10081 gcattgacta tgtcgtctga aaaggggctg aaaaatttat tttcaccaac actttttttg
    10141 ccacaacacg aagcggcgct ttttgctata acttagaaag taatataatc atctcaggaa
    10201 actattcatg cgtaccacat catttgcgaa agttgcagct ttatgcggct tgttggctct
    10261 gtctggttgt gcctctaaaa tcacccagcc agataaatat aacaattact ctgatttaaa
    10321 agaaacaacc tcagcaacag gtaaacctgt tttacgttgg gtagacccga gttttgatca
    10381 aagcaaatat gacagcatcg tctggaaccc aatcacttat tatccggtac caaaaccttc
    10441 tacccaggta gggcagaaag ttctggataa aattttgaac tataccaaca ccgaaatgaa
    10501 agaagcgata gcgcagcgta aaccagtggt taccaccgct gggccgcgta gtctgatttt
    10561 ccgtggggcc attaccggtg tagataccag caaagaaggg ctgcaattct atgaagtggt
    10621 tcctgttgca ttagtggttg cggggacgca aatggctaca ggccaccgta ccatggatac
    10681 tcgcctctat tttgaaggtg agctgattga tgcagcgact aataaaccgg ttatcaaagt
    10741 cgttcgtcag ggcgaaggta aagacctgaa taacgaaagt acgccaatgg ctttcgaaaa
    10801 tattaaacaa gttattgatg acatggcgac cgatgccacc atgtttgacg ttaacaaaaa
    10861 gtagttccag acgcgccatc gttagatggc gctttttatc cggtgcgccg taaaaccc
//
LOCUS       DAJTUP010000118        10883 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTUP010000118 DAJTUP010000000
VERSION     DAJTUP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10883
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.118"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            682..1704
                     /locus_tag="OO468_003349"
     CDS             682..1704
                     /locus_tag="OO468_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR6260343.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            1704..2684
                     /locus_tag="OO468_003350"
     CDS             1704..2684
                     /locus_tag="OO468_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR6260344.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            2681..3439
                     /locus_tag="OO468_003351"
     CDS             2681..3439
                     /locus_tag="OO468_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6260345.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            3449..4258
                     /locus_tag="OO468_003352"
     CDS             3449..4258
                     /locus_tag="OO468_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR6260346.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            4258..5112
                     /locus_tag="OO468_003353"
     CDS             4258..5112
                     /locus_tag="OO468_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR6260347.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            5138..5695
                     /locus_tag="OO468_003354"
     CDS             5138..5695
                     /locus_tag="OO468_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR6260348.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            5632..7092
                     /locus_tag="OO468_003355"
     CDS             5632..7092
                     /locus_tag="OO468_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR6260349.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(7142..7396)
                     /locus_tag="OO468_003356"
     CDS             complement(7142..7396)
                     /locus_tag="OO468_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR6260350.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7597..8331
                     /locus_tag="OO468_003357"
     CDS             7597..8331
                     /locus_tag="OO468_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR6260351.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            complement(8333..8944)
                     /gene="emtA"
                     /locus_tag="OO468_003358"
     CDS             complement(8333..8944)
                     /gene="emtA"
                     /locus_tag="OO468_003358"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR6260352.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            9044..9958
                     /gene="ldcA"
                     /locus_tag="OO468_003359"
     CDS             9044..9958
                     /gene="ldcA"
                     /locus_tag="OO468_003359"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR6260353.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            10053..>10883
                     /locus_tag="OO468_003360"
     CDS             10053..>10883
                     /locus_tag="OO468_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR6260354.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     L"
BASE COUNT         2614 a         2846 c         2697 g         2726 t
ORIGIN      
        1 tcattgctac accctgtgct taataactgc aactctccgc cgttcagcga tgctttgata
       61 attctgttcc catagatatc gttatcaaaa taaagcgtaa atatcatatt tccgtaactg
      121 ttatcatctc tttttttgca cgtccgaata aacgcattca gtaaattact ctctctaaga
      181 aaataaatat cttcattgtt gttaatgaca ttaataacgt cgttcttaaa atcctcaaag
      241 tcttgttgcg gtaacttctg gttacgctgc ttactatcag caagcggtga atccggacga
      301 caacaacata ggttttgtat aacccccagc attcttttca cctccgttaa cagtatattc
      361 acgacactga aacgagagaa tgctgataat tttctcggcg agatatcaaa aatcgctcat
      421 tataaaacaa gtaacttcaa cgtcgcgtaa ccgtggacga taaaaaaaca ataatgtcat
      481 tcatatttaa ataatttagc cttaaatgat ttaaataata ttagccacct gataaataca
      541 tttttaatga tagagagggt caacaggctt gcggaagtcg tcacatcttc atgttttcaa
      601 tagctaaact ttgatattga acatgatttt tatcgatata tatgaaagta tatatcgatt
      661 ttacttttca ggatgttcac catgcccata accagacgga cgtttgcgca ggcacttgca
      721 tctaccctgc tcctgcagtc tctaccgtcc ttttcacaaa cagttaatcg ttttgcttcg
      781 caatctcttc ccgaagccca aaacattaca cgcatcgtca gcgcaggcgc gcccgccgat
      841 ttgctcttgc tggctgtcgc gccagaaaaa atggtcggtt tttcctcttt tgattttgcc
      901 cgtcaggcat taattccatt gccagagcac attcgccagt tacccaggct gggacgactc
      961 gccgggcgcg ccagcacact atcgctggaa gggcttatgg cgttacatcc cgatttggtc
     1021 gttgattgcg gcaatacgga tgaaaccttg atctcccagg cacggcaggt tagcgaacag
     1081 acacaaatcc cctggttatt gcttaacgga aaactggcgc aatcagcaga acagttaaca
     1141 acgcttggca aaacgttagg cgaagagcac cgcgccgcag aacaagccaa tctcgccagc
     1201 cacttcgttg gtgaagctca ggcattcgcc acctcacccg ccgctaacct ccgcttttat
     1261 gctgcgcgcg gtccccgagg gctggaaacg ggcttacagg gttcgttgca taccgaggcg
     1321 gcggaattat taggtttgca caacgtcgcg caaatagccg atcgccacgg cctgacacag
     1381 gtttccatgg aaaatctcct gcgctggcag ccggatatta ttctggttca ggaggccgtt
     1441 actgcagatt ttattcgtcg tgatcctctc tggcagggcg tgaaagccgt tgcggaacaa
     1501 cgcatcttat ttttaagtgg cttgcccttt ggctggctcg atgccccgcc gggaatcaac
     1561 cgtcttctgg gattacgcag acttcacgcc tggctggatc ccgccatcaa tcgccagttt
     1621 aaaagtgaca tgcagcatta cgcccaactg ttctggcatt gttcactcag tgatgccgac
     1681 tatcaaaaat tggtggcgag ctaatgagaa tcgtaaacgg atgtattttg ctgctggcag
     1741 ctatcagtat cacttttgcc gccgtaagtg gggcttatca tctcgatatg cagcaactgc
     1801 tggcgctaat tcttcgtcaa gaaaatgagt ctgtgcagga acaaattgtc ttctggcaaa
     1861 tccgcgtgcc gcgtattctt gctgcgctgt tactgggcgc agcgctggca ggagccggaa
     1921 ccacctatca gggaatgttg cgtaatccct tagtttcacc ggatatttta ggcgtctccg
     1981 ccggtgccgg acttggtgcc tgcgcggcaa tcctgtgggg attacccatt gtttacatcc
     2041 agctgtacgc attttgcggc gggctgatgg tggtcgcggg cgtctggttg attacgcgtc
     2101 gggtgacccg ccacgatccg atcttaaccc tggtgctggt aggcattgcg ttaggaacgt
     2161 tgtgcggagc ggggatatcg ctaattaaaa cgctggccga tccttacacc cagttaccgt
     2221 caattacctt ctggttgctg gggggactgt ccaccgtgac cttacgcgat ctctgctatg
     2281 ctgcaccgat cattctcata ggctccttgc ccctgttttt tctccgctgg cgcatgaatt
     2341 tactcacgct ttccgacgac gaagcccgtt cgctgggact gaacgtgacg cgtctgcgct
     2401 ttggattgat tgtctgcgcc acactaatca ccgccagcac ggtagcaata gcgggcataa
     2461 ttggttggat cggtctggtc gttccgcaca ttgcccgctt gttgaccggt cataaccatc
     2521 agcagctgtt gcctatggca atgtgtactg gcgctattct gctcctgctt accgatacgc
     2581 tggcgcgcag cattggaaca acagaaatcc cacttggcat tctgaccgct tttgttggcg
     2641 caccgttttt cctgcttttg ttgttacgcg gaggtcgcca gtgactttgc ttgccgttcg
     2701 tcatgcgtct ctgggttact cccgccatcc agtgctacgc gacgtgtcct tcaccctgtc
     2761 gcaaggaaca atttgttgtc tgcttggcgc gaatggttgc ggcaaaacca cactcatgcg
     2821 ctccattctt ggcgttatac cgttattaaa aggcgagatc ctactggata gcctctctgt
     2881 gcagacactc agccatcgcc agcgcgcaca ggctattgcc tgggtgccgc aggcgcacga
     2941 cggtattttt gctttcagtg tgctggatat ggtgttgatg ggcctggccc ccaccatagg
     3001 cgctttttcc gttcccggta agcaagaacg cctgaaagcc acggagcaac tggaaaagct
     3061 gggcattttg catctggcga cacgacgctg gaatacgctt agcggcggtg aacgtcagct
     3121 ggctttaatc gcccgtgcac tcgtccaaca accgcgcttg ttgttactgg atgagcccgc
     3181 ctccagcctc gatttcggtc atcagatcca acttctggac acgctggcgc agctcaaaaa
     3241 taacggcatg acaatgttaa tgtcgactca ccatacgctc catgccaacg ccattgccga
     3301 cagcattatc caagtagagc cagatggacg agtaacacaa ggcttaccga ccgaacagtt
     3361 aacaacgaac aaactggcgg ccctgtatcg ggtttctgcc gaccaaattc atcatcacct
     3421 ctctgcaatt agccgctaag gaacaatcat gctcattgat gatattgatt ttgcagacct
     3481 ttatctgcag caactcaaac tggctcaccg cacagaaaaa acgcccgatc actgggatca
     3541 acgggcggag aaaatggcgg aaaattgcgc ctcgccaacg gacagctacc tgcaacaact
     3601 gatcgctaaa attgacctgc aaggcgctca gaccttattc gatatgggct gcggaccagg
     3661 caccgtatcg ctggcgctgg ctgacaaact cactacggtt tatggcgttg attacagtca
     3721 ggggatgctg aacgttgccg cccgccgcgc cgccgcactg aaggctgaca atgtccactg
     3781 gatccagcgc gcctgggaag aggactggag tgacctgcca cgctgcgata ttgccgtggc
     3841 ttcccgctcc actctggtgg ctgatatgcg ccatgcaatg agcaaactga ataatcaggc
     3901 acggcttcgc gtctatacaa ctcatctggt cagcacctcg ttcgtctctc cagccattca
     3961 gcgggcggct ggtcgggaag tgatcgaatt acccaactat atctttgcgc tcaacgtcct
     4021 gtatcagatg ggaatttatg cccacgttga ttttatccgt ggacaaaatt gccaacagga
     4081 taacagcacc tgggaacgtt ttgagcagaa cgtcagctgg agtcttggcg cgcttaacga
     4141 cgatgagcgt gagcggttat atcactggta tcaacaacaa gatgctcgtg ccctcgcccc
     4201 tgccagccgc gactgggcgc tcatctggtg ggatagcgta ccgcaggagg cactgtgatg
     4261 attttccttt ctcaggcaca aatcgatgcg ttactgctgg aagatatcca gggaggtgac
     4321 ctgaccacgc gagcattaaa tattggacac cagcctggct atatagcgtt ttttcaccgt
     4381 cagggcggct gcgtcagcgg cgtttctgtc gcgtgtaaga tgttaactac gctgggatta
     4441 actattgatg acgcggtcag cgacggttca caagcgaacg cgggtcaacg gctaatccgt
     4501 gcgcagggta atgccgcagc acttcatcag ggatggaagg cagtccagaa tgtgctggag
     4561 tggagttgcg gtgtttctga ttatctcgct caaatgctgg cattgcttcg tgaacgttat
     4621 cctgacggca atatcgcctg cacccgaaaa gcaattccgg gcacccgctt actggcttcg
     4681 caggcaattc tggctgccgg aggactaatt catcgtgccg gatgtgcgga aaccatatta
     4741 ttgtttgcca accaccgcca ttttcttcat gacaatcagg gctggtcagg cgcaatcgct
     4801 cagttacgtc gccacgcacc cgagaagaaa attgttgttg aagccgacac gccgaaagag
     4861 gctatcgccg cgttacgcgc acaaccagac gtacttcagc tcgacaaatt tagtccgcaa
     4921 caggcaactg aaattgcgca aatagcgccg tcgctggctc ctcactgcac gctggctctg
     4981 accggcggaa ttaatctgac gacactcaaa aattacctcg actgcggcat tcgccttttt
     5041 attacctccg cgccttatta cgcggcacca gctgacatta aagtcagtct gcaacccaca
     5101 gcctctattt aattctttgt taaacggaat ttattctatg agactcaaaa aacgttatct
     5161 ctgcacagca ttatcacttg cctttaccca gcaggccgta gcggctcagg agagcgacac
     5221 gctgaccgta tggtccagtc cggtatcatc gacgacgacc accgttctcg atcaacccac
     5281 catgaaggcc ttggataaac agaatgtcgc tcaggcatta agtgtcgtcc ccggcgtggt
     5341 gctgcaaaag tcaggcagcc gcaacgaaga acaggtaaaa gttcgtggct ttgatagtcg
     5401 gcaggtgccg gtctatttcg acggtgtgcc catttatgtc ccctatgacg gcaacctcga
     5461 tctggcgcgg attctgacca acaatctggg ggcagtcgaa gtttccaaag ggtattcgtc
     5521 gctgcttcat gggcctaatc agatgggcgg agccattaat atcaccaccc tgaagaagca
     5581 agtctgggat atcgccaggg atggagccgt agtcaggaca atgcctacga tatgcatgct
     5641 tcatttgccg ccagcagcga tcttgggtat ttgcaagtca gcggtagcca gctaaagcag
     5701 gattttctcg gcctgccgca tggtgtaaat aatgatattg caggcaaaca cggcaagatg
     5761 attaattcat cggctgatga taaacgcggc attgtgaagc tcggttttac accgcgcgaa
     5821 aacgatgaat acacgctgac ttacattaag caggatggtg aaaaagataa cccgccatac
     5881 agcggaaatt gtggtcaaaa atcacgctac tggcagtggc cagagtatga caaagaaagt
     5941 ttttattatc agggagcgac ccaactaaac gaccgtttta ccctgaaaag tcggctgtat
     6001 cgcgacacct ttgaaaatac gctgatgatg tacaactcgc tggctgattt gaaaaataaa
     6061 aaaggcagct acagccatta ttccgattac agcgacggtg ccggattaca actggcagcc
     6121 gatgtgcgtg aaaacgatct gctgtcgttt gccgttaact ggaaagatga cgtacaccgg
     6181 gaaaaaggtg cgccgcacgc cgcttacgat cgctatgaag atcgtacctg gtcgctcgcc
     6241 agtgaatatc aatgggctgc tgctgataat gtcgatgtcg tggcgggaat cagctatgac
     6301 tggcgcgata gcgtagaagc gaaaaaacac gagaaagatg gcagcatcac ccattatgac
     6361 gacaacaatc agtcagcttt taactggcag gtgatgggga aataccactt tgccaatgaa
     6421 gacactctgg cgctttcgta ctatgaccgc acacgctttc cgacgctgaa agaacgctat
     6481 accacatcca aacctgcgta taaccagata gcgattgtta acccgcagct caagccggaa
     6541 cgcgctcgcg gggtggattt aacctggaat ggtgccttca cgcgcgactg gggatttgag
     6601 gtcagcgttt actataaccg ggtgagtgat gccatcctct cgcacaatat caatgccgat
     6661 accattcaaa atcagaacag cggcacggtg gattacagcg gtctggatgc cggtattaag
     6721 gggaaaatca gcaatatact ggatgtagga ttgagctacg ctctgatcca cgctgacgcc
     6781 aaacgcaaag acatcggcaa gataaccgat cttccaacgc agacaatgac cgcatggatg
     6841 acactcaaac catgggagcc gttaagcgta acgctgtcgg aagaggcgcg ttcctccagc
     6901 tacagcaaca gtgacggttc acaaaaagcc gccggttttg cggtgaccca cattcgagcc
     6961 gattacacct taggtcatgg cttcagcgtc aatgcgtcgg tcaataacct gtttgatacc
     7021 caatatgcct acagtgaagg gtttattgaa gaaggtcgca atttctgggc aggtgtcgag
     7081 tatacgttct gatgtgaaag cggatagcgg catctgtatc gctatccgcc ttatgaaaat
     7141 cttattctct tcgcaggagg cggaatacgc ccagcaccag gatagcgccc accaccgcca
     7201 ccaggaagct gtgcaaatta aaaccgctga tggagccgtc aatgccaaac atggtcgcca
     7261 gccagccgcc gaccaccgca ccgactatcc cgagaataca ggtcaggaaa aatccaccac
     7321 catcacgccc cggcatgatt agcttgccga taatgccggc tatcaggcca aaaataatcc
     7381 aggcaataat tcccatgttc aggccctcgt ttcgatgaat cggtcgcaag aaatgcttac
     7441 gtcaacgctc taagtatagg caagggcggg atcgatgctg ggcgtttgtc ggctgattta
     7501 agaacaacat gtaaaaaata ttcacgcatt tgtattaagt tttgttaact atgaccgata
     7561 aaccaaagac agtttgtcag tcaggagttt ttccgcgtga gtcattacca tgagcagttc
     7621 ctgaaacaaa atccgttagc cgtcctgggc gtgttacgcg atttgcacaa agccgcaatt
     7681 cctttgcgtc tcagttggaa tggcgggcag ctgatcagca aaatactggc aataaccccg
     7741 gataaactgg tgctggattt cggcagtcaa gccgaagaca acatcgccgt gctaaaggca
     7801 cagcacatta ccattaccgc cgaaactcag ggtgcgaaag tcgagtttac tgttgaacaa
     7861 ctacagcaga gtgaatactt gcagcttccg gcatttatta ccgtaccgcc tcccacctta
     7921 tggtttgtac aacgacgccg atatttccgc atctccgccc cactccatcc gccttatttt
     7981 tgccagacca aactggcgga taacagtacg ttacgtttcc gcttgtatga tttgtcgtta
     8041 ggcggcatgg gcgcattact ggaaacagca aagcctgccg aattacaaga aggtatgcgc
     8101 ttcgctcaaa ttgaagtcaa catggggcaa tggggtgttt ttcactttga cgcccagtta
     8161 atctccacca gcgagcgcaa agtgattgat ggcaagaatg aaaccatcac cactccccgt
     8221 ctgagcttcc gttttcttaa cgtcagcccg acggtggagc ggcaattaca gcggattatt
     8281 ttctctctcg agcgagaagc ccgggaaaaa gcggacaaag tgcgcgactg atttacatcg
     8341 cgtccagtgc ctgctcaagt ttgtagatat agcgcggagc ctgcggcgca ggatgatttc
     8401 gcgctacgtg atcgaggaac tcgtcagcat ccaaatcgtt gattttgctg atcgcctttt
     8461 tccgatctga cgagaaagtc cgtagcagcg cacctgcccc gttagcgtat gacaccacca
     8521 gcgcatattg cagtaccttc ggatcttcaa tgcctgccag cgggccggtt tccagaatat
     8581 tcaggtaagc cgcccccatt gaaatattac gctctggatt tttcagctcg ctggtcgtcg
     8641 gctcaccact ccagcccata cggcgataaa catcacgtcc ggaggttgaa gcttttaact
     8701 gcatcaaacc aatggcattc gatttactca ccgcgttcgg attaccaccc gattcgatag
     8761 cgataatcgc cgtgatcaat tgtggatcga cgccccaggc tgcaccggct ttctggctta
     8821 ttggcatcca ctgcatcgca cgttgcaccg gaactttcgc gttccacggc gggttcgtat
     8881 agtcatgctt tgatgaacaa cccgctaata acacaatcaa aaaggcaaac catctcaatt
     8941 tcacttcttc tatccttata gcctggaatc gccgttgagg tgacaacggc acacctaagc
     9001 ggcatgatat acatttcgtt tctgttgtca gcaaggaatt gccatgtctc tgtttcactt
     9061 aattgcccca tcgggttaca gcattaaaca gcacgccgcg ctgcgtggta tccagcgcct
     9121 taccgacgcg gggcatcaag tcaataacgt cgaggttatt gcccgtcgtt gtgagcgttt
     9181 tgcgggtacg gaaacggagc gtctggaaga tctcaattcc cttgccagac tgactacccc
     9241 caacaccatc gtgctggctg tacgcggcgg ttacggtgcc agtcgtttac tggcagatat
     9301 tgactggcag gcactggtgg ctcgccaaca acatgacccg ttactcattt gcggacatag
     9361 cgattttacc gccattcagt gcggtcttct ggcgcaaggc aatgtcatca cctttagcgg
     9421 ccccatgctg gtagcaaact ttggtgcgga tgaactgaac gcctttaccg agcaccattt
     9481 ctggctggcg ttacgcaatg aaaccttcac cattgaatgg caaggggaag gcccgacatg
     9541 tcagaccgaa ggcacgttgt ggggaggcaa tcttgcgatg ctgatttcac tgattggtac
     9601 accgtggatg ccaaaaattg agaacggtat tctggtgctg gaagatatta acgtgcaccc
     9661 tttccgcgtc gaacgtatgc tgttgcagct ctatcatgcg ggaattttgc cacgtcagaa
     9721 ggcgattatt ctcggtagct ttagcggcag cacgcccaat gattatgacg caggttacaa
     9781 cctggagtca gtgtacgcgt ttttgcgttc ccgcctgtcg attccactca ttaccggcct
     9841 cgattttggt catgaacaac gcacggttac gctgccgttg ggcgcacatg ccattctgaa
     9901 taacacccgg gaaggcgctc agttgacgat tagcggtcat cctgttctta aaatgtaaaa
     9961 aaatcacgct tcagggctaa gtaaaacgct gtctctgccg ctaatatggt agggtttata
    10021 taagagacag cgtaatcagg agtaaccgac cattggatgc cacaacaata attagccttt
    10081 tcatcttagg atccatcctt gtcaccagca gtatattact tagttcattt tcttcccgtc
    10141 ttggcattcc tattctggtt atttttctgg cgatcggcat gctggcggga gtcgatggcg
    10201 tcggcggtat cccgtttgat aattacccct tcgcctacat ggtaagtaac ctggcgctgg
    10261 cgattatttt gctcgacggc gggatgcgca ctcaggccag ttcctttcgt gtggcgttag
    10321 gaccggcgct gtcgctggcg acgctgggcg tacttatcac ctctggttta accggcatga
    10381 tggcggcgtg gctgtttaat cttgatttga ttgaaggctt attaatcggc gctatcgtcg
    10441 gctccaccga tgctgcagcg gtcttttctc tgctgggtgg taaggggctt aacgaacgtg
    10501 ttggctcgac gctggaaatt gaatccggca gtaatgatcc aatggcggtc tttctgacga
    10561 ttaccctgat tgcaatgatc cagcagcatg aaagcagtgt cagttggatg ttcgtggtcg
    10621 atattctgca gcaatttggc ctcgggattg tcatcggtct tggcggtggt tatttactgc
    10681 tgcaaatgat taatcgcatc gccctgcctg ccggattata tccattgctg gcattaagcg
    10741 gcggtatttt aatttttgct ttaaccacgg cgctggaagg cagcggtatt ctggctgttt
    10801 atctgtgcgg ttttctgctg ggtaatcggc cgattcgcaa ccgctacggc atcctgcaaa
    10861 atttcgacgg cctcgcctgg ctg
//
LOCUS       DAJTUP010000119        10814 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTUP010000119 DAJTUP010000000
VERSION     DAJTUP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10814
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.119"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..285
                     /locus_tag="OO468_003361"
     CDS             <1..285
                     /locus_tag="OO468_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6260355.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWM
                     ESTIFPALNDVPALSGLIDTLNPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OO468_003362"
     CDS             296..1036
                     /locus_tag="OO468_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR6260356.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OO468_003363"
     CDS             1095..1481
                     /locus_tag="OO468_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR6260357.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OO468_003364"
     CDS             1490..1819
                     /locus_tag="OO468_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR6260358.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..4832
                     /locus_tag="OO468_003365"
     CDS             1791..4832
                     /locus_tag="OO468_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6260359.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4832..5161
                     /locus_tag="OO468_003366"
     CDS             4832..5161
                     /locus_tag="OO468_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6260360.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5161..5859
                     /locus_tag="OO468_003367"
     CDS             5161..5859
                     /locus_tag="OO468_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR6260361.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5864..6607
                     /locus_tag="OO468_003368"
     CDS             5864..6607
                     /locus_tag="OO468_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6260362.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6544..7146
                     /locus_tag="OO468_003369"
     CDS             6544..7146
                     /locus_tag="OO468_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR6260363.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7207..10686
                     /locus_tag="OO468_003370"
     CDS             7207..10686
                     /locus_tag="OO468_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR6260364.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10754..>10814
                     /locus_tag="OO468_003371"
     CDS             10754..>10814
                     /locus_tag="OO468_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HCR6260365.1"
                     /translation="MRKLYAAILSAAICLAVSGA"
BASE COUNT         2547 a         2563 c         3463 g         2241 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgctcag tacaccggaa aaatgacgga tgaagatgac
       61 tggcaggctg ttctgcatat tgccgtcttc atccgggcac aggcaccgga ttcagagctg
      121 gatatgtgga tggagagcac cattttcccg gccctgaatg atgtaccggc actttccgga
      181 ctcatcgaca ccctgaaccc actcggtttt aactatcaac gtgataatga gatggccacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg
     4621 atggtaagct gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc
     4681 agtacaacat tgagatcaat aacgacggca cgaacgggca gataggtccg gctgccctga
     4741 aggtggttta tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg
     4801 atggtggccg gttaagtgga gcataccggt aatggagacg tttcactgga aagtgcgccc
     4861 ggatatgaat gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga
     4921 acagcgtcgt gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt
     4981 tcgtaaaggt gaacacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc
     5041 atttcagtgg acgccacctt atgactggaa actgatcagg gtggtttgtc gtaaatggtc
     5101 ggcaagcgtg ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg
     5161 atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcagcccgg
     5221 gtggagctct gggaaatcga tctgacagag gtcggtggtg agcgttattt tttctgtaat
     5281 gagcagaacg aaaaaggtga gccggtcacc tggcaggggc ggcagtatca ggcatacccc
     5341 attcaggggt cggggtttga actgaacggc aggggctgtg ctgcccgtcc gacactgacg
     5401 gtctctaacc tgcacggcat ggtcaccggg atggcggaag atctgcagag tctggtcggc
     5461 ggaacggtgg tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac
     5521 ggaaacagcg acgccgatcc ggaacaggag gtgatcagcc gctggcgcat cgagcagtgc
     5581 agcgaactga gcgcggtcag tgcctctttt gtgctggcca caccaacgga gacggatggc
     5641 gcggttttcc cggggcgtat catgctggcg aatacctgta tgtggactta ccgttctgat
     5701 gagtgtggtt acacgggcag ggcagtggct gacgagttcg acaaaccaac aacggatatc
     5761 cggaaggaca aatgcagcaa gtgtatgcgc gggtgtgagt tgcgcaacaa taccggtaat
     5821 ttcggcggtt tcctttccat caataaactt tctcagtaaa tccatgacac agacagaatc
     5881 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgtggct tcgtggtgag
     5941 aacgccggag ggagacaggt atcttcccag cgagaatatc tccggtgagc cggaggaacg
     6001 gttccggatg gctccggagg actggctgcg ggcacaaatg cagggtgaga ttgtggcact
     6061 ggtccacagt catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt
     6121 gcagagtgat ttgccgtggt ggctggtctg ccgggggacg attcacaagt tccgctgtgt
     6181 gccgcatctc accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     6241 ggatgcttat catctggcgg ggattgagat gccggatttt catcgcgggg atgactggtg
     6301 gcgtcacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc
     6361 gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa
     6421 tcatgccgcc atttactgcg gcgatggtga gctgctgcac catattcctg aacaactgag
     6481 taaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
     6541 ggaatggcac gcatctgcct ttacggggat ttgcaacgat ttggccgccg catcgacctt
     6601 cgtgtgaaaa cgggggctga agccatccgc gcactggcca cacagctccc ggcgtttcgt
     6661 cagaaactga gtgacggctg gtatcaggtg cgcattgccg ggcgtgatgc aggtgaaacc
     6721 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg cagtgatcca catagtaccg
     6781 cgtctggtgg gagctaaaag tggcggtgtg tttcaggcgg tgctgggggc agctgttatg
     6841 gcggttgcta tatggatgcc gggggtagga attatggcga gtaatctgct gttttctctc
     6901 ggtgccagta tgacgcttgg cggtgttgca cagatgctgg caccgaaagc cagaactccc
     6961 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
     7021 gttgcccagg gcaatgttct gcctgttctg tacggtgaaa tgcgcgtggg gtcgcgggtg
     7081 gtatctcagg agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt
     7141 cgctgatgca aaatgtttca tgtgaaaccg cctgcgggcg gttttgtcgt ttatggagcg
     7201 tgaggaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
     7261 aaatccacgc agttgctgag tgtgattgat gccatcagcg aagggccggt tgacggtccg
     7321 gtggatggat taaaaagcgt gctgctgaac ggtacgccgg tcctggatag cgaggggaag
     7381 acaaactttt ccggtgttac ggtggtgttc cgcgccggcg agcaggagca gacaccgccg
     7441 gaggggtttg aatcttccgg ctcagagact gtgctgggta cggaagtgaa atacgacacg
     7501 ccgatcaccc ggaccatcac gtcggcaaac attgatcgtc tgcgcctgac cttcggtgtg
     7561 caggcactgg tggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     7621 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     7681 accacttcgc agtatctggc ctcggtggtg gtgggtaacc tgccgccgcg cccgtttaat
     7741 atccggatgc gcaggatgac accggacagc accacagacc agctgcagaa caaaacgctc
     7801 tggtcgtcgt acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgctggtc
     7861 ggcgtgcagg tggattcaga gcagttcggt aaccagcagg tgagtcgcaa ttatcatctt
     7921 cgcgggcgca ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt
     7981 atctgggacg gaacgcttaa gccagcatac agcaacaaca tggcctggtg tctgtgggac
     8041 atgctcactc atccgcgcta cggcatgggg aaacgtcttg gtgcggcaga tgtggacaaa
     8101 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg atttggcggc
     8161 acggagccgc gcatcacctg taacgcttac ctgaccacac agcgtaaggc gtgggatgtt
     8221 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     8281 ttcgtgcagg accgaccgtc ggataaagtg tggacctata accgcagtaa tgtggtgatg
     8341 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgtc
     8401 gttgaggtga actggattga tccggacaac ggtcatgaga cggcgacaga gcttgtggag
     8461 gacacgcagg ccattgtccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     8521 accagccggg gacaggcgca ccgcgccggg ctgtggctga ttaaaacgga actgctggaa
     8581 acgcagaccg tggacttcag cgtcggcgca gaagggcttc gccatgtacc gggtgatgtc
     8641 attgaaatct gcgatgatga ttatgccggt atcagcaccg gtggtcgcgt actggcggtg
     8701 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccgtc ctccggtacc
     8761 acgctgataa gcctggttga cggaaatggc aatccggtca gcgtggaggt tcagtccgtc
     8821 accgacggcg tgaaggtgaa agtgagccgg gttcctgacg gcgttgcaga atacagcgtg
     8881 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     8941 aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg tgccggaaaa agaggccatc
     9001 gtggataacg gggcacactt tgacggtgac cggcgcggca cggtgaatgg tgtcacgccg
     9061 ccagcggtgc agcatctgac cgccgaagtc accgcagaca gcggggaata tcaggtgctg
     9121 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     9181 gcagcggacg acggcagtga gcggctggtc agcacagcca ggacgacgga aaccacatac
     9241 cgcttcaggc agctggcgct ggggaattac agtctgacag tccgggcggt aaatgcccgg
     9301 gggcagcagg gcgatccggc gtcggtatcg ttccggattg cggcaccggc agcgcctgtc
     9361 actattgaac tgataccggg gtattttcag ataacggtgg tcccgaaact ggctgtatat
     9421 gacccgacgg tgcagtttga gttctggttt tcggaaaagc ggattgcgga tatcaggcag
     9481 gttgaaacca gcgcccgcta tcttggcacg gcgctgtact ggatagccgc cagtatcaat
     9541 atcaggccgg gccatgatta ttatttttac gttcgcagtg tgaacaccgt tggcaaatcg
     9601 gcatttgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct gagtttttat
     9661 aaagggttga tcaataaaac gcatctcggc aaggagttgt ggacgcagat tgataacggt
     9721 cagcttgcgc cggacctgac tgaaatcagg acgtccatta cgaatgtcag caatgaaatc
     9781 acgcaaaccg tcaataaaaa actggaaaat cagagtgctg caatccagca gatacagaaa
     9841 gttcaggttg atacaaataa taacctgaac agcatgtggg ctgtgaagct gcaacagatg
     9901 aaggacggac gcctttatat tgcgggtatc ggtgccggta ttgagaatac gccagcagga
     9961 atgcagagtc aggtgctgct ggcggcagac aggattgcga tgattaatcc tgcgaatggc
    10021 aacacaaagc cgatgtttgt tggtcagggc gatcagatat tcatgaacga cgtgttcctg
    10081 aaacgcctga cggctccgac cattaccagc ggcggtaatc ctccggcatt ttccctgaca
    10141 cctggcggac ggctgacggc gaaaaatgcc gatatcagcg gtaacgtgaa cgcgaactcc
    10201 gggacgctca acaacgtcac gattaataag aactgtcggg ttctgggaaa attgtccgcg
    10261 aaccagattg aaggcgatct cgttaaaaca gtgggcaaac ctttcccacg ggactcccgg
    10321 gcaccggaga ggtggccatc agggaccatt accgtcaggg tttatgacga tcagccgttt
    10381 gatcggcaaa ttgttattcc cgcggtggcg tttcgcggtg ctaaacatga gcggaagaat
    10441 aacaatattt attcgtcatg ccgcctgata gtgaagaaaa acggtgctga aatttataac
    10501 cgaacgaccc tggataatac gctgatatat acgggtgtta ttgatatgcc tgccggtcac
    10561 ggtcacatga cgctggagtt ttctgtatcg gcatggctgg taaatggctg gtatcccaca
    10621 gcaagtatca gcgatttgct ggttgttgtg atgaagaaat ccacagcagg tatcagtatc
    10681 agctgaattt tataacccag atacgggcac cagaaatggt gcctttttta ttgcagaaaa
    10741 gcgagaggta attatgcgta aactttatgc tgccattttg tccgcagcca tctgtctggc
    10801 tgtatccggt gcgc
//
LOCUS       DAJTUP010000120        10701 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTUP010000120 DAJTUP010000000
VERSION     DAJTUP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.120"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..152)
                     /locus_tag="OO468_003372"
     CDS             complement(<1..152)
                     /locus_tag="OO468_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="cytosolic phosphatase which functions in the
                     chemotaxis signal transduction complex by controlling the
                     level of phosphorylated CheY through dephosphorylation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6260366.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDA"
     gene            complement(163..552)
                     /gene="cheY"
                     /locus_tag="OO468_003373"
     CDS             complement(163..552)
                     /gene="cheY"
                     /locus_tag="OO468_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR6260367.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(567..1616)
                     /gene="cheB"
                     /locus_tag="OO468_003374"
     CDS             complement(567..1616)
                     /gene="cheB"
                     /locus_tag="OO468_003374"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR6260368.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(1619..2479)
                     /gene="cheR"
                     /locus_tag="OO468_003375"
     CDS             complement(1619..2479)
                     /gene="cheR"
                     /locus_tag="OO468_003375"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR6260369.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(2498..4099)
                     /gene="tap"
                     /locus_tag="OO468_003376"
     CDS             complement(2498..4099)
                     /gene="tap"
                     /locus_tag="OO468_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR6260370.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(4145..5806)
                     /gene="tar"
                     /locus_tag="OO468_003377"
     CDS             complement(4145..5806)
                     /gene="tar"
                     /locus_tag="OO468_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR6260371.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(5951..6454)
                     /gene="cheW"
                     /locus_tag="OO468_003378"
     CDS             complement(5951..6454)
                     /gene="cheW"
                     /locus_tag="OO468_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR6260372.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(6475..8439)
                     /gene="cheA"
                     /locus_tag="OO468_003379"
     CDS             complement(6475..8439)
                     /gene="cheA"
                     /locus_tag="OO468_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR6260373.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(8444..9370)
                     /gene="motB"
                     /locus_tag="OO468_003380"
     CDS             complement(8444..9370)
                     /gene="motB"
                     /locus_tag="OO468_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR6260374.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(9367..10254)
                     /gene="motA"
                     /locus_tag="OO468_003381"
     CDS             complement(9367..10254)
                     /gene="motA"
                     /locus_tag="OO468_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR6260375.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(10381..>10701)
                     /locus_tag="OO468_003382"
     CDS             complement(10381..>10701)
                     /locus_tag="OO468_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="With FlhD is involved in the activation of class 2
                     flagellar genes and as well as a number of other genetic
                     systems; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FlhC"
                     /protein_id="HCR6260376.1"
                     /translation="GLCNGVDAVIKAYRLYLEQCPQAEEGPLLALTRAWTLVRFVESG
                     LLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPSRAVKRRKLSQNPADIIPQLLDEQR
                     VQAV"
BASE COUNT         2423 a         3021 c         2630 g         2627 t
ORIGIN      
        1 gcatcgggga tggcttccgc cgcttcggca atggcctgaa ccagccccag ttcccgcaaa
       61 ctgtcgcgca gcatatgcgt caggctgccg atgcgcgcaa tgatatcgcc agctgaatgc
      121 tcgtcagcag gtttgattga tggttgcatc atagtcgcat cctcacatgc ccagtttctc
      181 aaagattttg ttgagttttt cctccagcgt cgcggcggta aatggcttca ccacatagcc
      241 actggccccc gcttgcgccg cagcaatgat gttctctttc ttcgcttctg cagtcaccat
      301 taacactggc aatgccgaca tcgcgccatc cgcacgaatt gttttcagca attccaggcc
      361 atccatattg ggcatgttcc agtcggagat aacaaatcca taaccgcctg cctgcaactt
      421 attgagagcg tcgacaccat cttccgcttc ctcaacatta ttgaatccca gctctttcag
      481 caggttacgc actatgcgtc gcatggtgga aaagtcatcc acaaccaaaa atttaagttc
      541 tttatccgcc atttcacact cctgatttaa atacgtatcg cctgtccggc actaattgtt
      601 gccaacattt gctggcttac ctggctaaga tcgaccactt cgcagacacc acccatattg
      661 atggcctcgc gcggcatgcc gaacaccacg caacttgctt cgttttgcgc aagggtccat
      721 gcccccgcct gacgcatcgc caacattccc gccgcgccgt cgttgcccat accggtcagg
      781 atcaccccaa ccgcattacg ccccgcctgt ttggcgacag aatggaacaa cacatctacc
      841 gaaggccgat gacggttaac cgccgggccg tcgtgaattt tgatttgata atttgcgcca
      901 ctacgcgcca gttccatatg ccgatcgccc ggcgcaatat aggcatgccc cggcaagaca
      961 cgttctccgt cttcggcttc tttaaccccg atctggcaaa gcttattaag tctgtcggca
     1021 aaagagcggg tgaaaccggg cggcatatgt tgggtaatta acaatgccgg gctggaaagc
     1081 ggcaacggtt gcagtacgtg acgaattgcc tcagttccac ccgttgaagc accaatcgca
     1141 atcagttttt cagaactcaa caacggccct gccttcagcg ttgtcggtgc tgacaatggc
     1201 ttatgtgctg caaggctcgc ctttgctgcc gtacgcacct tttcagcaat catttcgcta
     1261 tacgccagca ttccttcgcg aatacccagt tgcggtttgg tgacaaaatc tattgccccc
     1321 agctccagcg cgcgcagcgt gacttctgat cctttgccgg tcaaggaaga aaccatcaca
     1381 acgggcattg gacgcaaacg cattaatttt tcgaggaaat ccagtccgtc catccgcggc
     1441 atttcaacat ccagcgtcag cacatcggga ttgaatttct taatcaagtc acgcgcaacc
     1501 agcggatcag gcgcggtcgc caccatttcc atgtcgctat ggctgttgat gatttctgtc
     1561 atgatctggc gcatcagtgc cgaatcatcg acagataaca ccctgatttt gctcatcgtt
     1621 aatccttact tagcgcatac accgtctgac cacgcagcgt gaagcggcgc tcgaggtggc
     1681 taaagttttc agagtgaccc gcaaacagca atccgtcggg tttaaggagc ggaacaaagc
     1741 ggcgcaaaat ctcctgctgg gtagtttgat cgaagtagat catgacgtta cgacagaaga
     1801 tcgcatcaaa cggccccggc acggtgtact gtttcgccag taaattcagc ggggcaaaat
     1861 caacatagtt cgccagctcc tgacgcacgc gtaccagccc ttcatgcggt cccgtccctc
     1921 gcatgaaata ccgttgcagt tgctgcggcg tcaggttttt caactcttca tggcgataga
     1981 taccgcttct ggctttttcc agcacttcgg tgtcgatatc actggcaaac actttccagc
     2041 gtccgggcac ggtgcccaat gtgtcagcca gagtcatcgc aatgctgtac ggctcttcgc
     2101 cggtcgaagc cgccgcgctc catacgcgat cctcgccaga acggcgacgt gcgtgatccg
     2161 cgagcagagg gaaatgatga gcctcacgga aaaatgccgt cagattcgtg gtcagcgaat
     2221 tgataaacgc ctgccactca ccgctgtgct gattagattc cagcaagttc agataatgac
     2281 cgaaatccgt cagtcccaga gaacgcaaac gacgaaccag tcggttgtaa accatgtcgc
     2341 gtttatggtc agccagaacg atcccggctc gttgatagat caattgactt atccgccgaa
     2401 aatgcgcgtc ggaaagcgcc aggcgctcgg tcatctgtaa caataaagac gtttgcccac
     2461 agggcagaga tgaagtcata gcgccttctc aatcacttca ggataccact ggcgcaattt
     2521 gtaactgcgc cgactcatgt cgtgctactt catgttcttc aagggtaaat accgccacac
     2581 gcgacgaaag atggtcggcc tgattcgcca gttgttccgt tgccaccgcc gcttcttcta
     2641 ccagcgagga gttctgctga gtcacctgat ccatctggct gacagcctgt gcaacctgct
     2701 caatcccccg ccgttgttct tccgacgccg aagcaatttc tcccataatg tcgttcacgc
     2761 gggtcaccga actgacaata tcggtcatgg tcgcggcggc gttattcacc agtttcgaac
     2821 cctgctggac acgattgact gactcttcga tcagcccttt gatctctttt gctgcctgcg
     2881 cgctacggct ggcaagattg cgcacttcac ctgccactac cgcaaaacca cgcccctgct
     2941 ctccggcgcg agccgcttcc actgccgcat tcagggccag aatattggtc tggaaagcaa
     3001 ttccgtcgat aacgctgata atgtcgccaa ttttttgcga gctggtggcg atctcctgca
     3061 tggtgtgagt catggtactg acctgaacac cgcccgcctg cgccgttgtc gcggcatttt
     3121 ttgccagttc cgacgcctgt cgtgcgttat cggcgttttg ccctaccgtg gcggttaatt
     3181 gctccatact ggcggccgtt tgtgccagcg atgccgcctg ctgttcggta cgacttgaga
     3241 gatcgttatt gcctgcgaca atctccgcga tcccaatgtg catctcatgg cttcccttac
     3301 gcacatcact caccgtccca cgcaaagcct gctgcatggt cttcaggctg gcaaaaatgg
     3361 cggtgatctc attacgacca tataccgcaa tcggacgcgc cagattaccc gcagcaatgc
     3421 tgtcaaaatg gctaccgata atggccagtg gttgaacaat catcttgcgc gtccaccaca
     3481 gcgcactgct gatgtagatc gccgcaacaa taatcatgct gataaacacc agcgccgaaa
     3541 tctgatagtt acgctggctt tgcgcactgg cggcttccag cacatggttg atctccagct
     3601 gccaggcctc aaagttaacg tcaaacgcat tctgtgatcc ctgcaccggc gcagtgagga
     3661 aatccgaaag ttgattactt tcaagccagg tcgcctgatg ttcgagatcg ttgtgccagc
     3721 gggcaaaact tttctccgtc tctttttgca atgccctgac gtgctcgttg cccgcagtca
     3781 tcgccataaa acttttaaac agcgtggtgg attgcgtcag actggcgcgc gccgtcgtca
     3841 tcaacgtttt aatgtcatcc gccggatagc taagcgctgt cagagtgcct gctttgttca
     3901 gcgcggtact ggcctgtaac attaccgccc gagtttgcgc taatgccgca cgttgctgat
     3961 tgctctgctc gccgtgattc agtcgttgca aatcgtcgcg aaatgcccaa aaagacatgc
     4021 cgttactgcc aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa
     4081 ttcgaatacg attaaacatc aacgctcccc atcaggcggc aatgaccgcg ttagtaaaaa
     4141 ctcgtcaaaa tgtttcccag tttggatctt gttcagtaat tcgcagtcgt ggctgtgccg
     4201 gtggttgctc actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg
     4261 ccagacggaa cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg
     4321 cggcggcagc tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg
     4381 aaaccgccaa tgcgacttga tcgatgccac ggctctgttc atccgatgcc gatgcaatct
     4441 cgcccataat gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccgg
     4501 cgctttcgac cagcaccgaa ccggtatcaa tgcgcgagac ggagtcttca atgagggctt
     4561 tgatctcttt tgccgccagg gcgctgcgac tggcaagatt acgcacttca cccgccacca
     4621 cggcaaaacc acggccctgt tcacccgcac gcgcggcttc aaccgcggca ttcagcgcga
     4681 ggatattagt ctggaaggca ataccgtcga taacgctgat aatgtcggca attttcttcg
     4741 aactatcggc gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct
     4801 gggcggtgtc ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt
     4861 gcttcactgt cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct
     4921 gctgttcagt acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat
     4981 agatggcatc tgaaccttcg cggacatgag tgacggtgtc agtcaaagag cgttgcatat
     5041 gtgaaacgct ctgcgccagg tcacccattt cactgcgccc gtcaatggtc agggtattcg
     5101 ccaggttacc accggcgatt tcgcaaatgt gagcaataat ttttgccagc ggagtaagca
     5161 acatacggcg aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa
     5221 ccgccagttg ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat
     5281 acagtttttc actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc
     5341 cctgggttgg ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt
     5401 cagttaacgc tgtgtaatag tttttatatt tttcatcaaa attacggctg gtagcgacca
     5461 tttcaggtaa cggtgccatg cttttgaatt ttttataatg cgtcgcggcc tgggccaatg
     5521 ttttcctggc gctatcgagc aattcaactt tggcgttact ttgttgattg gaggaatcca
     5581 tcatcatccg caccgctgaa cgactcaggt taatgcgcgt ttgcagcatt aaatcccagg
     5641 ttgacgtcag ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc
     5701 tatggtgaag ggaagaaaaa aacaaactgc cggaaataag ctgtaacagt gcgaataccc
     5761 ccagcaccat taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg
     5821 aaaacaagtt gatctcgtta tcggcaagaa ggggggaaac tttattgctg atgccacccg
     5881 ccgcgaaatt gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa
     5941 atcgggagaa ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt
     6001 tcagcagttt ttcgatgttc accagaatca acatacggtc gcccagtgcg cccagtccag
     6061 tgagatattc tgttgaaagc gtcacggcaa actctggtgc cggacgaatt tgctccgccg
     6121 tcaatgaaag cacgtctgaa acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat
     6181 tcaggacgat aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc
     6241 gtaagtcaac aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg
     6301 gcgtgttcgc aatccgtgtt acctgatcgt agccacggat ctcctgcact ttcaggatat
     6361 caataccgta ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc
     6421 tggccagctt tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc
     6481 ggcggtgttc gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat
     6541 cagtgccacg ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata
     6601 gttactttca aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa
     6661 ggcgtagcgg cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt
     6721 cgcgcccgcg acgttgaaca ctttccacag ttcgacgatg ggcagatatt caccccgcac
     6781 ttccagcacc cgctcgccgc cggccagtgg atggagatcg gcttcacggg gttgcagtga
     6841 ttccataaca gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc
     6901 gtcgaggatg gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt
     6961 cgactggatt tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc
     7021 gacgccgcgc ccggagacgt cggtgacctg ctctgccgtg gagaagcctg gtgcaaatat
     7081 cagcattgca acttcgtcgt cgctcatgtt ttcgctgaca gtcaaacctt gcgaggccgc
     7141 ttttgccaga attcgctcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat
     7201 gttgccgccc tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcggc
     7261 gagccgtttt tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag
     7321 cgggtcgata atgcgttcta tcaggctctt gtcgagctcg gtggaactgc ccaccagcgt
     7381 cagttctacc tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact
     7441 aaagacatat tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgggc
     7501 gttacgttgt aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc
     7561 gctggaacgc tgggcaagca tggactgggt gataaccagc tcgccgacga ggttaattaa
     7621 ttgatcaacc ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt
     7681 ttcccgctcc acgcggccgg ttggcgcttg ttcggctgcc agtttaagca caggtggggt
     7741 agctattttt ggcgaaactt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa
     7801 acagagtacc gctgtgatgt catcttcggc gatatcgccc ggtaatattg ccgagagaga
     7861 atccgcccct ttcaccacgt catttaacgt tgtcagatgc cccagctctt cttccagcag
     7921 gtcgacttcg ccggccttca ggcgcgaaag gataattcgt cgcggcaact gactgcgact
     7981 ttgctcatct tgcggttcac ttttggcaac cacacttaat cgggtcactg cggatggcgt
     8041 ttcgcctttc gcttctaatg ccagttgacg caaggcctgg cagatataat cgtagctggc
     8101 ggcatccggc tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt
     8161 ttccaaaaac agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc
     8221 gagcaggttt tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aagttcctgc
     8281 ccctcctttg atcgagtggg cagcccgaaa gatggcattc aattgttcgg catctggcgc
     8341 ttccggctgc aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc
     8401 aaaaaatgtc tgataaaaat cgcttatatc gatgctcacg ctgtcacctc ggttcggctg
     8461 atggcattgt gggaacactg acctgtggtg caacctcagg tttttctagg gcgcttactg
     8521 gctcattctg gctttcggcg ttttcatgca aaatggcctg ttcggcttgt ttgttcagta
     8581 ccaacaggct gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg
     8641 ttgccgccat gccgacgaca cgtaacactt tgccgctatc caaccctccg accatcagtt
     8701 cgcggcggga tgcattggcc cgatcggcag aaagctccca gttgctgtaa cctttctcac
     8761 cgctggcgta gggaaaatca tcggtatgac ctgaaaggct aatacggttg ggaataccgt
     8821 tcagcacagg cgcgatggcg cgcagaatgt cgcgcatata gggttcgaca tcggcactgc
     8881 cggttctaaa catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga
     8941 ccagatcgat tttgagatgg agacgtaacg cccgcagttt cggatcggac tctatcaact
     9001 ggtcgagatc accccgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt
     9061 cgatgttcgg ctgcttattc acttccccct ggctttgggt gtaatcatca ccaccgccgg
     9121 gaattgggct ttcactattg gaaatgcgat cgccgcccgt aaccgctgtc gccagtggag
     9181 tccggaagta ctccgcaatc tgaatcaact cttttgggct ggagatggag atcagccaca
     9241 tcaccagaaa aaaggccatc atcgcagtca taaagtcggc ataagcaatc ttccacgatc
     9301 catgtgctgc cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt
     9361 gattcttcat gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg
     9421 ctcttccagt tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc
     9481 aacggcgata ggcggtgcgt aaccgttcag attagaaagc agagtgactt tgacgcactg
     9541 catcattttg ctggtttcgg cgcttttctg acgtaaaaca ctcgctagtg gggaaataaa
     9601 tccgtaagcc aataaaatac cgaggaaagt ccccaccatc gcatgtgcga taagcgcccc
     9661 cagctcggcg gcaggacgat cggctgaacc taacgcgtga acgcccccca ttacagccgc
     9721 gacaatacca aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc
     9781 tgcctcgctt tcgtgcgtct caatctcttc atccatcagg gcttcgattt cgaaggtgtt
     9841 catgtgaccg ctgataatca ggcgcagata atcgacgata aaatcaagca tgacgctatc
     9901 cgcgaggatg cgtgggtagc tggcgaagat ctcgctctca cggggatttt caatatcacg
     9961 ttccagcgaa aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag
    10021 cagatccata tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcttt
    10081 cagcgtgcct ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc
    10141 ggcaataatc accagttcag cgggttgata gagtgctcca aggcttccac cggtcatcaa
    10201 ataaccgccg aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc
    10261 atccttccac tgttgaccat gacaggatgt tcagtcgtca ggcgttaacg cgcgattggg
    10321 gaaaaaaaaa gcagcggtac gtcgttaccg ctgctggaat gttgcgccac accgtatcag
    10381 ttaaacagcc tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg
    10441 ggaaagttta cgtcttttta ctgcccggga tggcggttga cataagctgc aggcaaagct
    10501 gccaacaggc tggtgagcgt gggtaataaa attgccgccg cagcagttgc agctggaaag
    10561 ttgcagtaat ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag
    10621 tggtccttct tctgcttgtg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc
    10681 atcgacgcca ttacacaaac c
//
LOCUS       DAJTUP010000121        10563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTUP010000121 DAJTUP010000000
VERSION     DAJTUP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.121"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO468_003383"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO468_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR6260377.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO468_003384"
     tRNA            complement(621..695)
                     /locus_tag="OO468_003384"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO468_003385"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO468_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO468_003386"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO468_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR6260378.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO468_003387"
     CDS             complement(2932..3990)
                     /locus_tag="OO468_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260379.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO468_003388"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO468_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR6260380.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO468_003389"
     CDS             6068..6352
                     /locus_tag="OO468_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR6260381.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO468_003390"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO468_003390"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR6260382.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO468_003391"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO468_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR6260383.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..>10563
                     /gene="sixA"
                     /locus_tag="OO468_003392"
     CDS             10189..>10563
                     /gene="sixA"
                     /locus_tag="OO468_003392"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR6260384.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETP"
BASE COUNT         2611 a         2451 c         2654 g         2847 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccg
//
LOCUS       DAJTUP010000122        10554 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTUP010000122 DAJTUP010000000
VERSION     DAJTUP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.122"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            43..1497
                     /gene="amn"
                     /locus_tag="OO468_003393"
     CDS             43..1497
                     /gene="amn"
                     /locus_tag="OO468_003393"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR6260385.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            1840..2556
                     /locus_tag="OO468_003394"
     CDS             1840..2556
                     /locus_tag="OO468_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR6260386.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3012..3087)
                     /locus_tag="OO468_003395"
     tRNA            complement(3012..3087)
                     /locus_tag="OO468_003395"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3052..3054),aa:Asn,seq:gtt)
     gene            complement(3188..4815)
                     /gene="yeeO"
                     /locus_tag="OO468_003396"
                     /pseudo
     CDS             complement(3188..4815)
                     /gene="yeeO"
                     /locus_tag="OO468_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            4820..4895
                     /locus_tag="OO468_003397"
     tRNA            4820..4895
                     /locus_tag="OO468_003397"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4853..4855,aa:Asn,seq:gtt)
     gene            complement(4933..5883)
                     /gene="cbl"
                     /locus_tag="OO468_003398"
     CDS             complement(4933..5883)
                     /gene="cbl"
                     /locus_tag="OO468_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR6260387.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(5985..6902)
                     /gene="nac"
                     /locus_tag="OO468_003399"
     CDS             complement(5985..6902)
                     /gene="nac"
                     /locus_tag="OO468_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR6260388.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7230..7305
                     /locus_tag="OO468_003400"
     tRNA            7230..7305
                     /locus_tag="OO468_003400"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7263..7265,aa:Asn,seq:gtt)
     gene            complement(7361..8296)
                     /gene="ldtA"
                     /locus_tag="OO468_003401"
     CDS             complement(7361..8296)
                     /gene="ldtA"
                     /locus_tag="OO468_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6260389.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8358..9437)
                     /gene="cobT"
                     /locus_tag="OO468_003402"
     CDS             complement(8358..9437)
                     /gene="cobT"
                     /locus_tag="OO468_003402"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR6260390.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9449..10192)
                     /gene="cobS"
                     /locus_tag="OO468_003403"
     CDS             complement(9449..10192)
                     /gene="cobS"
                     /locus_tag="OO468_003403"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR6260391.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10189..>10554)
                     /locus_tag="OO468_003404"
     CDS             complement(10189..>10554)
                     /locus_tag="OO468_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR6260392.1"
                     /translation="WQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWD
                     YQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQL
                     AAAANEVWLVVSGIGVKIK"
BASE COUNT         2963 a         2687 c         2271 g         2633 t
ORIGIN      
        1 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
       61 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      121 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      181 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      241 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      301 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      361 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      421 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      481 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      541 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      601 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      661 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
      721 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
      781 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
      841 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
      901 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
      961 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1021 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1081 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1141 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1201 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1261 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1321 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1381 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1441 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1501 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1561 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1621 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1681 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     1741 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     1801 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     1861 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     1921 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     1981 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2041 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2101 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2161 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2221 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2281 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2341 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2401 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2461 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2521 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
     2581 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca
     2641 tattgcttat aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt
     2701 ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga
     2761 cagctacgtc ccgcataagg catttttctc tttatctttg tacggtactt catggaacag
     2821 aatttttgac cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg
     2881 tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa
     2941 gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca
     3001 aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
     3061 ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc
     3121 acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt
     3181 ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggctcg gatcgagggt
     3241 atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc
     3301 agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg attccctgca
     3361 cataaccgac tacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac
     3421 gggcatcacg agcaccctta aatccagcgg gtagcaccca tgaggcggac caaataggca
     3481 taaataaagc atttagctaa atcagaatca caacgacgtg tttaacctgt ggatcctggg
     3541 tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc
     3601 caagagtgga cagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta
     3661 cccccaacct tcggcctgta atgatcgtag aagcagaacc gagcgcactt ccgggtaagt
     3721 tgataagagc cgcaattgag aacgcgataa aatttccggc aataacgctg gtccccatcc
     3781 cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg
     3841 ctggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc
     3901 tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa
     3961 ctgcgccaat ataacgagaa atggttaaac ccaacccagc cccgaccagg agaaaaggcc
     4021 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     4081 cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc
     4141 ggctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacttctgt
     4201 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc
     4261 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     4321 tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc
     4381 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc
     4441 atcttttccc agccaactga ccagaaaagt gctcaggacc cccatcaaca ggacacaggc
     4501 gttctccatg aagataggaa cagcaagcgg ggttatctcg cgctagaaca acactttgta
     4561 gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     4621 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc
     4681 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     4741 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa
     4801 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     4861 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     4921 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg
     4981 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc aaacatagtt
     5041 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     5101 cgtatccagg cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa
     5161 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gtgcacttaa
     5221 tacaatatct gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg agcgccccgt
     5281 aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     5341 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac aaaaccatgg
     5401 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     5461 gccattttgc aacaacgtcg caatttcctg tggcgaaccc tggattagct cgagccgaac
     5521 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     5581 atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg
     5641 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     5701 ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caaattcatc
     5761 ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac
     5821 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     5881 cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata
     5941 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     6001 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     6061 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     6121 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     6181 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     6241 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     6301 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     6361 acatcaacgt tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     6421 aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     6481 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     6541 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     6601 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     6661 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt
     6721 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     6781 ccttccagta tggcaacctg ttggctgagc gctggttgtg cgatatgcaa tacttcagca
     6841 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     6901 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagtatgc
     6961 aagatgcctg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga
     7021 gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa
     7081 aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     7141 tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc ctttgacatc accatgcact
     7201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     7261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     7321 gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     7381 cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt gcgcgattta
     7441 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     7501 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     7561 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     7621 ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct
     7681 gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     7741 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     7801 aactctcccc tcgtttcgca tattcgcgcc ggatgttcgg cgttggcgtc caggttggtg
     7861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     7921 cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca
     7981 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     8041 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     8101 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     8161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     8221 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat
     8281 ttacacgacg catcatgtcc ctttcctaag tcgcgaatac tatccgataa gtatagcttt
     8341 tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata
     8401 ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatggac
     8461 atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc
     8521 agccccaaat gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg agaaggaatg
     8581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     8641 aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt
     8701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcctg aggatttggt
     8761 tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc
     8821 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     8881 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     8941 tttttcgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     9001 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     9061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc
     9121 gcttgtgctg ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg
     9181 gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     9241 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcacccccgg cattcctgcc
     9301 agttgtatgg caagcgcctc cagctttcct aggctaccaa caggtttgaa taacccgtca
     9361 acatgccgtt gtgcacgcga catagcggca gaattgatgg ctgggatcgt attcagtaaa
     9421 tcggcaatta tttgcatgtc acgtttcttt atagcagtgc cagcaggaag accagttcac
     9481 caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa
     9541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     9601 ccagcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     9661 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
     9721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     9781 tcggttcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
     9841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     9901 cgcgactacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatt
     9961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt gcgccacacc
    10021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaatcaaag
    10081 gaaaagtaat aataccgcga gaataatgtt cgaaatccag tccctgggac cagcgacgcg
    10141 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttgctc atttgatttt
    10201 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
    10261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
    10321 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
    10381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
    10441 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
    10501 cacagcctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gcca
//
LOCUS       DAJTUP010000123        10547 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTUP010000123 DAJTUP010000000
VERSION     DAJTUP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10547
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.123"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            61..411
                     /locus_tag="OO468_003405"
     CDS             61..411
                     /locus_tag="OO468_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR6260393.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            464..859
                     /locus_tag="OO468_003406"
     CDS             464..859
                     /locus_tag="OO468_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR6260394.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            900..1643
                     /gene="cmoA"
                     /locus_tag="OO468_003407"
     CDS             900..1643
                     /gene="cmoA"
                     /locus_tag="OO468_003407"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR6260395.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1640..2611
                     /gene="cmoB"
                     /locus_tag="OO468_003408"
     CDS             1640..2611
                     /gene="cmoB"
                     /locus_tag="OO468_003408"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR6260396.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2776..5205)
                     /gene="torZ"
                     /locus_tag="OO468_003409"
     CDS             complement(2776..5205)
                     /gene="torZ"
                     /locus_tag="OO468_003409"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR6260397.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5230..6330)
                     /locus_tag="OO468_003410"
     CDS             complement(5230..6330)
                     /locus_tag="OO468_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR6260398.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6718..7464)
                     /gene="cutC"
                     /locus_tag="OO468_003411"
     CDS             complement(6718..7464)
                     /gene="cutC"
                     /locus_tag="OO468_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR6260399.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7478..8044)
                     /locus_tag="OO468_003412"
     CDS             complement(7478..8044)
                     /locus_tag="OO468_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR6260400.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8260..9993
                     /gene="argS"
                     /locus_tag="OO468_003413"
     CDS             8260..9993
                     /gene="argS"
                     /locus_tag="OO468_003413"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR6260401.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10170..>10547
                     /locus_tag="OO468_003414"
     CDS             10170..>10547
                     /locus_tag="OO468_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR6260402.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYS"
BASE COUNT         2562 a         2703 c         2543 g         2739 t
ORIGIN      
        1 ctatcataac cactagcatc acctttagga accatctgaa acgggaggtg tatttagacc
       61 ttgctaatct tagacagccg cccggttcat gcatcacgtc cacacagtga agctattcgc
      121 gacgctcaac gaaaaaaacc taaagttccg gtacatgctg tactgacggc gacaaatccg
      181 ctgatccgtt ttatcggtag tgatgatatg acgcaaaacc gggaattatt tcaggtctgg
      241 ttacaaaaat tagcgcagtg gcatcagacc actacgcctt atcttttttt acatacgcca
      301 gatattgccc aggccccgga actggtacat accctgtggg aagacttacg taaaacgctt
      361 ccagagatcg gagcagttcc ggctattcca cagcaatctt ctcttttctg aatttgccac
      421 ctatcataga caggtgccat cggccatttt aaagggagtt tgtatggtaa gcgcgctata
      481 tgccgtttta agtgcgttgt tattaatgaa gttctctttt gatgtcgttc gcctgcgaat
      541 gcagtaccgc gttgcctatg gtgacggtgg ttttagcgaa ctgcaaagcg ctatttgcat
      601 tcatggtaac gcggtggaat atattcccat cgcgattgtg ctgatgctgt ttatggaaat
      661 gaatggcgca gaaacctgga tggtgcatat ttgcggcatc gttttgcttg ctggtcgtct
      721 gatgcattat tacggttttc atcaccgtct gtttcgctgg cgacgttccg gcatgagcgc
      781 cacctggtgt gcgctgttgc tgatggtgct ggcgaatctt tggtatatgc cctgggagtt
      841 ggttttctcc ctgcgttagc gcacaatacg ccactttctt tttcccggat ttttacgtta
      901 tgtctcaccg cgacacgcta ttttctgccc ctatcgccag actgggcgac tggacctttg
      961 atgaacgggt cgctgaagtc ttcccggata tgatccagcg ttccgttccc ggctattcca
     1021 atattatttc catgattggt atgttagccg agcgcttcgt tcaacctggt acgcaggttt
     1081 acgatctggg ttgttctctg ggcgcggcga cgctctcggt gcgtcgcaac attcatcatg
     1141 ataattgcaa aattattgcc atcgacaact ccccggcgat gattgaacgc tgccgtcgtc
     1201 atattgacgc ctataaagcc cctacgccag tagacgtcat tgaaggtgat attcgcgata
     1261 tcgccattga aaacgcatcg atggtggtgc tgaattttac cctgcaattc ctggaacctt
     1321 ccgagcgcca ggcattactg gataaaattt atcaaggact gaacccgggc ggcgcgctgg
     1381 tgctttcgga aaaattcagt ttcgaagatg ccaaagttgg tgaactgttg ttcaacatgc
     1441 accacgactt taaacgtgcc aacggttaca gcgaactgga gatcagccag aagcgcagca
     1501 tgctggaaaa cgtgatgctg accgattccg tggaaaccca taaagcacgc ctgcataaag
     1561 ccggttttga gcatagcgag ctgtggttcc agtgctttaa ctttggttca ctggtggcat
     1621 taaaagcaga ggacgctgca tgatcgactt tggtaacttt tattctctga ttgccaaaaa
     1681 tcatctttca cactggctcg aaacgctgcc cgcgcagatt gctaactggc agcgcgagca
     1741 gcagcacggg ctgtttaagc agtggtccaa cacggtggaa tttctgcctg aaattaaacc
     1801 gtatcgtctg gatttattgc atagcgtaac cgccgaaagc gaagagccac tgagcaccgg
     1861 gcaaattaaa cgcattgaaa cgcttatgcg gaacctgatg ccatggcgca aagggccgtt
     1921 ctcactgtat ggcgtcaaca tcgataccga atggcgttcc gactggaaat gggatcgtgt
     1981 tctgccccat ctttctgatt taaccggacg caccattctg gatgtcggct gcggcagcgg
     2041 ttatcacatg tggcgcatga ttggcgcagg cgcgcacctc gcggtgggga tcgaccctac
     2101 gcaactgttt ctgtgccagt ttgaagcggt gcgtaaacta ctgggtaacg atcagcgcgc
     2161 acatttgtta ccgttaggta ttgaacagct tccggcactg aaagcctttg ataccgtctt
     2221 ttcgatgggc gtgctttacc atcgccgttc gccgctggag catctctggc agttgaaaga
     2281 tcaactggtg aatgagggtg aactggtgct ggaaacgctg gttattgatg gcgatgagaa
     2341 cacggtgctg gtaccaggcg atcgttacgc gcaaatgcgt aatgtctatt tcatcccttc
     2401 cgcgctggcg ctgaaaaact ggctgaagaa gtgtggtttt gtagatatcc gcattgtgga
     2461 tgtgtgcgtt accactactg aagagcagcg acgcactgaa tggatggtta ccgaatctct
     2521 gtccgatttc ctcgacccac atgatccgag taagacagtt gaaggttatc ctgcgcctaa
     2581 acgcgcggtg ctgattgcgc gcaagccgta aaggtctggt attactgccg gatgcggcgt
     2641 gaacgcctta tccggcctac aaacccttgc taattcaatc tattgcaggg actatgtagg
     2701 cctgataagc atagcgcatc aggcagtttt acgtttgcat aacctcagcg cccgtttccg
     2761 ggcgctattc acttcttacg cctgaacagc aggcttatca aatgccgtta acttcggtgc
     2821 gttacccgta tatttttcga tatacaccag cgcagagtta ccggcacagg catttgcccg
     2881 ctgcgagctg gggatatccg ccgttaacac gttcgcactg ccgtttttac acaagccgtt
     2941 ttccagatct ggccatgcac cttcatgcac gcataccacg ccttttttga tcccgtcagt
     3001 caccaccgcg cctgtcaaaa tctgaccacg tttgttccag acacgcacca gatcgccatt
     3061 cgcaatacca aatcgagcag catcttcggt gtgaatagtg atcggttcac gatcggcaac
     3121 cgcatatttt ttacgcagtt ccgcatagtt aagctgactg tgtaaacggt gtgccggatg
     3181 tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc ttccactcat caggcgcaag
     3241 ccaggttggg tgtgccgggc aatccttata gccaaatttt tccagcgttt tggagtaaat
     3301 ctcaattttg ccgcttggcg tacccagcgc atttttcacc ggatcggcgc ggaaatcacc
     3361 ataacgaacg tactgttcgt tcttctcgct gcggcgcatt tcgatcagtt tattttgctg
     3421 ccagaaggca ttaaacattg gcatagtgac gcgttgcgca cgggcccctt tctgagcggc
     3481 atcatagaaa aatttcagcc acgccatttc atctttacct tcggtataga tctcttttcc
     3541 gccaggtttg agtaattccg caagatcggc aaacacgtca aaatcgttac gcgcttcaaa
     3601 ttgcggagca acagcctgct tcatcggcac aatatgctgg ttgctgtaat caccggtcat
     3661 cgtcaagtca ttgcgctcaa acgatgtggt gatcggtaat acgatatctg cgtgtttggc
     3721 tgctgcggtc cagtagcatt cagaaacgac gatcatctcc ggtttctgcc aggctttgat
     3781 cagacgatta gtatcctggt gatgggtaaa gttaccgcca cccgcccacc agatcatttt
     3841 gatattcgga tacgtttgtt ctttaccgtt atgctgatat ttgccgccag gattttccag
     3901 cgcatcgacg atacgggcaa caggaatcgc cgtcattccg ccatcatcag cggcctcgct
     3961 ggcctgtccg gcaatcgccg cagacatttc cggcagcacg ccgccaacac gcgtcgggtt
     4021 gccaccattg gagtaatgat aagagaaacc aaaaccgccg cccggtgtac caatctgccc
     4081 taacatcgcc gccagcgtca ccagcatcca gtgtttctgt tcaccatatt gctggcgctg
     4141 aattccccag cctgccatca gcattgtgcg attcgccgcc atcagttcag cgagtttgac
     4201 tatctgcgct tccggtacgc cagtaatttc cgccgtccag accgcgctct tcggcgtatt
     4261 atcgctctta cctgtcagat actcttcaaa ctgcggatac ccggtagtgt atttttcaag
     4321 gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc gcaatcccta acatcagtgc
     4381 cacgtcggtg cccatattcg gcgcgatcca ggtggcatta tcgccaaaga attcgatggt
     4441 ttcggagcgg ataggatcaa tggcaatcac tggtttgcca gattttttca gctgatggaa
     4501 gtattccagc ccttgctcat cggtactgct ccaggcaatt tttaaggtat tcagcgggtt
     4561 cattccccac agcaccacaa cctggctgtt ttccagaatc agcggccagg aggcctgctg
     4621 ttcatacacc tctacagaac cgaccacatg cggcatgatc acctgtgctg ccccggttga
     4681 ataatcgccg ctatgcccgg aataaccgcc cgccaggttc atataacgtt gcagtaaggt
     4741 ttgcgcttta tgcaacacgc cagaagagcg ccagccgtaa gatccggcaa aaatggccga
     4801 tggtccgtta gctttacgaa tacgctcatg ttgctcatga atcagtttta atgcctgttc
     4861 ccagctcacc tgtacatagg tatcttcgcc acgacctttc gccggttgca gtggattgtc
     4921 gagatagctt tttctcacca tcggatgctg aatacgcgcc gtggtgtgta cctgatccgc
     4981 cgccgtagac tgtaaggagt tcggtatggt tttcgccagc gcgcctgtcg aagaaacaat
     5041 cttgccgtcc ttcacttcta cgctcatcgc tccccaacgt cccgcggtga ggattttacc
     5101 gcccttctct tctgcccatg cggggagcgg tgctgccgat gtcaccacca gcgctccagc
     5161 ggcaataccg ctgtgtttaa taaattcacg tcttgttaat gccataactt cctccctgat
     5221 caacgaggat cactgtttct cggtaatatc tttggcgttg tactggaaat accgcgttaa
     5281 aatgtccggt tcgttttcgc tcatgctggt tcgtgccccc attcctttgg caatggacgg
     5341 ccacgcattg acggtgtaat ggtcggcggc aatagaggca tgacaaccag cgcaataggt
     5401 atcgtcaagt ttttcagcgt attgccatag cggtttacgg tccgctaatg cgggatcggt
     5461 aagcgcaccc tgtaaagacg cctgacgcca ttgattgccg tattcgtcag cctgccattc
     5521 cccgtttacc gtgagcgcct tgataccttc ttcacttaat gtggctagcg ccagccgttg
     5581 acctgccgcc aggtagagcg tgttttcact gccctgcatt tgataaccct gcaacagaac
     5641 gatcggctgt ttgccactgg catcaacgac ggtgagatcg gtaccaggat tcacggtagc
     5701 cagctcgcct atgcgagaag ttttgaaagg ataaatatgt gcgccattag tcactgaagt
     5761 agcggcctga ctttccagct catgcgccgc gttgtcatcc atttttattt ctggcggaaa
     5821 atgggcaatg cctttatgac aatcgataca ggtttcgccg cccttttgtg ctttgttatg
     5881 cattttctgc gcagattcac tttgcgaggc aatatccatg gcatcaaaag aatggcaact
     5941 acggcacgtt gcagagtcag ttgcttttaa ttctttccat actgtttcgg ccatttcctg
     6001 gcgatgagtt tcgaacttat cgtcactgtc tattttgccg ctaacaaatt catgataaat
     6061 atctttagat gcttttaatt tagcaaataa ataatccatc cctgactttg gaatatggca
     6121 atcggcacat tccgcacgta tccctttctg gttcgaaaag tggacagttc cctgatattc
     6181 ctcaaaaggt ttactcatcg agtggcagga aaggcaaaat gctgtatccg acgttttatg
     6241 taaggctttt tgcgccagca ataacccgcc gccaccaacc acaaccgcta tcagcaaaaa
     6301 caataaccca atgcgttttt tccctcgcat agattcctct aaataaattc cagaacaccg
     6361 aaatcattgt ataattaaat acacaacgaa ttaaccgctt gcataactcg gcacaacact
     6421 gcctgaaata atcgataaag aatatgattt attacaatgt aatcattaat tgctaaggaa
     6481 taacccagtt gctatttaga atatatattt atcagtttta gcaatttaaa tcccataatc
     6541 aatgtgaata taaaaaatat ttttagcaca acgctatgcg cactgtcacc cgctcaacag
     6601 atcacggtta tcgttcgttt tttatactgc tcagggataa aaaaaggccc ctgttgaaat
     6661 tgcaggggcc tggtacgagc aagcatcata ttgggcgaca tgatgcaacg gtaaaaatca
     6721 tttggcctga tggcgttcaa tgattccttt catttcagca accgccgccc cgtctacgat
     6781 ataacgcgaa tactcgtccg cgtgttcatc tgatgacatg gacaatcctt gattacgata
     6841 acgcatcggt gaggcttgcc acgctcccgc ggagctatgg acttccagca ctccggcatc
     6901 gaggaagtgg tgcaggtttt ctgcacggac tcctgctcca gccataatga ttggagcatc
     6961 acgatgggca ataagttcca taatttttga taaaccttgc agtgcgtctg atttttgccc
     7021 tgatgtcagt acccgggcaa tgcccaattc cgcaagatta ttgagtgtat ataaagggtt
     7081 agcgcacata tcgaaggcgc gatgaaaagt cactgccagc ggaccggcag cagccattat
     7141 tttttccatt cgtggcatat cgacattccc gtcaacatcg agaacgcccg tcaccagtcc
     7201 aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga atggcggcaa actcaccgtc
     7261 gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga tgcacaggga tcgtcacccg
     7321 ctggcgcacg gatttcagta cccccaacga cggcgttaag cccccctctt ttggggctgc
     7381 gcataattca actctgtctg cgccgttttg ctgcgccgtt agtgcacatt ccatgctgta
     7441 acagcaaatt tccagtaatg ccatttttgc tccttaatta cgctgactgc tcgctggcaa
     7501 cgatctcttc aatggaccac ggatgaaact taatagttgt tttgccatcg gtgaccgcca
     7561 gcgtcggatt cggtaatcgc tcatgctcac cttttggtga actggtttta actgagatcc
     7621 ccggtaggct gagtccttca tcagaaagca atgccagcgc acgggcattc agcgtttctg
     7681 gatcacccgg cagaacgatt tcaatatgtt cccaaccttc gtgtgggtaa cgtttttccc
     7741 cgggccacgg tagctccaca ttggaaaact gccagtgcgc aacctgtacc ggttcatgca
     7801 atttaaacag acaaatcggt ctgccattga tcattttttc tgacaaaagc tcgccacact
     7861 gttcaaaacc gcgacgccag cgctcagcgg tgacgttttg atggcaacgc aaagaaatgt
     7921 gatcggcagt cagcggagtg atatccaacc ccagacggcg ggaaagttca tctaatgcgt
     7981 gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc gtcgatagat tgccagttcg
     8041 ccataatcac tcttcgtctt tcagtaaaag cgttaattta ccctgttgcc ctgtgccaac
     8101 caaccgctga tttcacgccg cttctgatgc aatagtgaaa acggcaatac gccacgcgca
     8161 cgttgctgac gaaaacagcc atttgcagta tactcccgcc ctaatttctt taactggtgc
     8221 gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg tgaatattca ggctcttctc
     8281 tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag
     8341 attcgtcagt cagcaaaagt tcagttcggc gactatcagg ctaacggcat gatggcagtt
     8401 gctaaaaaac tgggtatggc accgcgacaa ttagcagagc aggtgctgac tcatctggat
     8461 cttaacggta tcgccagcaa agttgagatc gccggtccag gctttatcaa cattttcctt
     8521 gatccggcat tcctggctga acatgttcag caggcgctgg cgtccgatcg tctcggtgtt
     8581 gctacgccag aaaaacagac cattgtggtt gactactctg cgccaaacgt ggcgaaagag
     8641 atgcacgtcg gtcacctgcg ctctaccatt attggtgaca cagcagtgcg tactctggag
     8701 ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg actggggcac tcagttcggt
     8761 atgctgattg catggctgga aaagcagcag caggaaaacg ccggtgaaat ggagctggct
     8821 gaccttgaag gtttctaccg cgatgcgaaa aagcattacg acgaagatga agagtttgcc
     8881 gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg acgaatattt ccgcgagatg
     8941 tggcgcaaac tggtcgacat caccatgacg cagaaccaga tcacttacga tcgtctcaac
     9001 gtgacgctga cccgtgatga cgtgatgggc gaaagcctct acaacccaat gctgccagga
     9061 attgtggcgg atctcaaagc caaaggtctg gcagtagaaa gcgaaggggc gaccgtcgta
     9121 ttccttgatg tgtttaaaaa caaggaaggc gaaccgatgg gcgtgatcat tcagaagaaa
     9181 gatggcggct atctctacac caccactgat atcgcctgtg cgaaatatcg ttatgaaaca
     9241 ctgcatgccg atcgcgtgct gtattacatc gactcccgtc agcatcaaca cctgatgcag
     9301 gcatgggcga tcgtccgtaa agcaggctat gtaccggaat ccgtaccgct ggaacaccac
     9361 atgttcggca tgatgctggg taaagacggc aaaccgttca aaacccgcgc gggtggtacc
     9421 gtgaaactgg cagacctgct ggatgaagcc ctggagcgtg cacgtcgtct ggtggcagaa
     9481 aagaacccgg atatgccagc tgacgagctg gaaaaactgg ctaacgcggt tggtattggt
     9541 gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg actacatctt cgactgggac
     9601 aacatgctgg cgtttgaggg taataccgcg ccatacatgc agtatgctta cacgcgtgta
     9661 ttgtccgtgt tccgtaaagc agaaattaac gaagagcaac tggctgcagc tccggtaatc
     9721 atccgtgaag atcgtgaagc gcaactggca gctcgcctgc tgcagtttga agaaaccctc
     9781 accgtggttg cccgtgaagg cacgccgcat gtaatgtgtg cttacctgta cgatctggcc
     9841 ggtctgttct ctggcttcta cgagcactgc ccgatcctca gcgcagaaaa cgaagaagtg
     9901 cgtaacagcc gtctgaaact ggcacaactg acagcgaaga cgctgaagct gggtctggat
     9961 acgctgggta ttgagactgt agagcgtatg taatcgattt ttcgtgagag tgaagcctga
    10021 tcagggttag ccgatcaggc ttttttattg ccatctaaat gtattctgaa aacggacatg
    10081 ccattgtttt ctcactgttg agtaagaggc cagaagcgta atatccaggc ccaggaaaac
    10141 gataacggtt caatttaagg aataccgaag tgtttaaatt tcttgtatta acattaggca
    10201 ttatctcttg ccaggcttac gcagaagata cggttatagt aaacgaccat gacacttcag
    10261 ccatcaaaga ttgttggcaa aaaaattcag atgatgatac tgacattaac gtgatcaaat
    10321 catgcctgcg acaagaatac aatctcgtcg atgcgcaatt aaataaagcc tatggtgaag
    10381 cttatcgtta tatagaacaa gtgccacgca caggtgcaaa aaaacttgat actgaacaac
    10441 ttaacttgct taaaaaatca cagcgagcct ggctggattt tagggacaaa gaatgtgaat
    10501 taatcctttc aaatgaggat gttcaggatt taagtgaccc ctattct
//
LOCUS       DAJTUP010000124        10368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTUP010000124 DAJTUP010000000
VERSION     DAJTUP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.124"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..260)
                     /locus_tag="OO468_003415"
     CDS             complement(<1..260)
                     /locus_tag="OO468_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6260403.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNN"
     gene            complement(354..916)
                     /locus_tag="OO468_003416"
                     /pseudo
     CDS             complement(354..916)
                     /locus_tag="OO468_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1120..1326)
                     /locus_tag="OO468_003417"
     CDS             complement(1120..1326)
                     /locus_tag="OO468_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR6260404.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1433..1996)
                     /locus_tag="OO468_003418"
     CDS             complement(1433..1996)
                     /locus_tag="OO468_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR6260405.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3371..5206)
                     /locus_tag="OO468_003419"
     CDS             complement(3371..5206)
                     /locus_tag="OO468_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260406.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5307..5594
                     /locus_tag="OO468_003420"
     CDS             5307..5594
                     /locus_tag="OO468_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR6260407.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5566..7095
                     /locus_tag="OO468_003421"
     CDS             5566..7095
                     /locus_tag="OO468_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR6260408.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7265..8482)
                     /locus_tag="OO468_003422"
     CDS             complement(7265..8482)
                     /locus_tag="OO468_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6260409.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8849..9835)
                     /locus_tag="OO468_003423"
     CDS             complement(8849..9835)
                     /locus_tag="OO468_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260410.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9832..10368)
                     /locus_tag="OO468_003424"
                     /pseudo
     CDS             complement(9832..10368)
                     /locus_tag="OO468_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3227 a         2369 c         1949 g         2823 t
ORIGIN      
        1 tcgttattaa gtagcagcac cataagataa ggagtggcat cgttagccac agcttcccta
       61 ctccagaggt aatataaagg ggtgggctcg actgatttat ctttacgtcg cttacactgc
      121 aaatgttcag aaattagtct atgcagctca ccagtaaaat cagcaatcag agaaggaatt
      181 gccttattca taccagggca aggtattaat ttaaactgta ataatttaat tttaggatgt
      241 gtagcagcag cccgatacag agttgcaagg acacactttt gccagagggc gttacaggaa
      301 agcttaatgt ttgattctgt atacataata aatcacctta tagttacaat aggtcaaaaa
      361 ccgctgtagc caaagttacg ctggcctgat gctttagtgt cgagcttcgt caaataatcc
      421 agacgtacca ataagcgctg gtactgctct gggaaattag gatcgttaat atccaggatg
      481 taacgtccat tagcagggaa atgaactaat ccctggtgat cttccagttc caaccctaag
      541 gcactgtacc aggctccggt aattaaccct ctcagggtgt cagagcgctc atagtttccc
      601 agatggtaat aggcgtcttt gttaaaaaaa ggcagacgtg ataatgacat ttaaaggact
      661 gcgtatattc tttcgcccag ataatacaaa ccgacgttgc gtaggtgcgt ttgccttgtt
      721 gttttttacg gtgttgatct gcattaattt tcgcattcag cgagttacgg aaacgagata
      781 tctctccggg gatcaggtta gggaaacagg taatgatatc cccgttgtca atcacaggag
      841 ggtaatggag atccccccgt attgctgtta ttctggaagt gtttttaata ttcgtattga
      901 tagttctcag agatatatca cactctagat attttaatta agtatcagtt cgatatcggc
      961 atattgtaca ctacctcagg tagatgatga gatcagatac agggatcaac tgacggtgag
     1021 cgcagtcccc acattttatc cagggcttac tgcatattcc caacagccat tcgttagcca
     1081 ggtagacgaa taccctgatt tgccgctggt tttgctttcc tatcgaaggg cccatacatc
     1141 atcacgccca cgaaacaacc gacgaaataa cgcaactttc tcatccgtgg ataatacgaa
     1201 aacgcactgc acagggctct gcggtttacg tcgccattca atcccatgag cttcaagtaa
     1261 tgagaccagc cgggtatttt ctgcccgcag tactgctagt tcatcgctgt cagatttata
     1321 aaccatatgc gttatcactt gtacctgtct cccggcggca ccacgactga aattcgtgtg
     1381 aaggcaaatc cacgcgataa cttacgatag ttcggacata agcccccata attcagttat
     1441 gtcgtggtcg accccggtta cttttttcgg gaagcctgct acggcccaga ataataccgt
     1501 gttctttctg atcctgttta gccagatagc taagggcgta acgtaagtcg tctaccgcag
     1561 atttatcact atagtgaatc acgtgatcga tacgcaccgg gtatttgtct ttagcccggc
     1621 acagatgaaa ataaccctcc ccttccgtga tccgcctcca gatatctccc agttgcctgg
     1681 atatctgata agagttcttg tgacgttgtc catccaggta gccgataaaa tgaatatgaa
     1741 gccctttgtc tggtgtatat tccattaccc agtaatatcc cgccaacatc gtctgggttt
     1801 cgctaagtaa acggtatatt tccatacaca tactgtgctt acaggaatgc ccgaaactgg
     1861 gcgtgtcttt cctgtaagca aaatcaattc tgaaaggtaa cagtttagaa aaacgttgaa
     1921 acataccatc catgtgttca ttcacgtctt tcagaatcat gaaatccatt ttgtaattgg
     1981 gattggcatt atacatttta tagtccttac tttataaaac tcacaggaag agagaattac
     2041 gtaaatactg agagtagaga aacgccgtac cacggcatta ataaacgaag taaaccagca
     2101 gcacagacaa cctgataaaa ctgttttgta ttaccgcatt actgtgatga ggtaatactt
     2161 cagaatacag tgagactctc ttaaactgag aaccagtacg aattacaatg gaaaaacaca
     2221 cctcatagaa ttgagtttca ggaagcacca ctaaattctt tacaggaagt aatctgacat
     2281 tcacaaaact cagtatcggc tagctacagg caacatcgct attaatattg tcgaaccatt
     2341 aagtaaagcc gagagagacc acgggtattt aatcattagc ataaaagaat aatggtatac
     2401 agaacatacc cacacaccat aagaagattg ttccatacag aaaagatcat atttacctat
     2461 acctataaac tgtaacggag tcataacacg ctctaatagt gttccgatac gtaaccagtg
     2521 ttaagtacgt acaggacaac attacacaca taattcacca tacagcttcc cccatatgct
     2581 tcactgcgta gtgtatggag atatatgcat tacacacata cagaatactt agcataaaga
     2641 ggagtgacat taccacgtaa agagataagt tatacaacaa acatacaaac aatacggcac
     2701 agacacacaa taccataact ccaactaata atactaatta accttaatta tttatggtat
     2761 atgaaataaa tgtgattctc ccggaactaa ctaacctaac cagacagatg atcactgtat
     2821 aaaaacttca gttaatactc atttgttttg tcgactcagt atataatgag tgaccagtaa
     2881 tcaggataat cacacttaca taacctgtaa aaaagaacaa gaagaataag ccactaacat
     2941 aatgcaaaat ggcatctgaa tcgatcgcag tttggcattc gaatcgatca tgatttggcc
     3001 caatccgtga tcgaatagag cggaatacgc aacataattc accagacaag agcttacacc
     3061 gaattacaga ttatgtatta atgtcaaaac tggcatattt tacagataca atgaacatgc
     3121 aacccatcaa tctaataggg gcggtatgta catctgccca tgaatgggat ggcgctaaga
     3181 atacaaaaca attagacata gatcacaaaa acacaccatt ctaattgaaa aacaaaacaa
     3241 acaaataaat catactatta tagaattttc tcttgccaga catagataat acccctgcta
     3301 attttgctaa tggttagcaa aattagcagg gtatataaag attattgacc caaaacacac
     3361 tcagatgaat tcacgacagc tcaggagggt tattcctgat gtcatcttca caaataatgg
     3421 ttaacgcatc atatatcatt ctgcgtctgt tcctgttttc ggccttatcg ccatacaaca
     3481 tcatcacatt tttaatttca tcgaccggtg atttcacata gtgaactaat acaccaagct
     3541 tctcaacaca ttgcagtaaa gtatatttct tactgcctgt gaaaagccct atcatagttt
     3601 ctttacgttc gcgactggat aatactttgt tttccataat gacttctgcc agcgtcttac
     3661 catcataaac atactcttta tctatcagaa tatttatctt atccagcacc ttctctacgt
     3721 catcaagcaa acctgtaata tatgggggcg caatatatgg tttcgtactg atcttttcaa
     3781 gcatacaatt atacatctta ataacacgca tcagggtaaa attgttcgca tcatactgta
     3841 tttcaccctc cttatataca ggatcaataa gtggtatttt ttcaataaat gcatcctgat
     3901 aggcttttac atggtcaagc caataaaaca acgagccata ctttatattt tttctattta
     3961 ctttaacctc cagtgctgtc cgaagcaacg ataatgcccc cctgacatat cctgaaggta
     4021 attcaccaaa caatcctttt ctttccatgt catcataatg ctgcagcaca tcattaattt
     4081 tatttttcct tatcagacaa atgtacaccg caagtgtttc gtagcagctg gtcacatgaa
     4141 cagcctcatt ctgcaactgc ccaaaatagc ttataatctt tttcagatat gcgtcatcac
     4201 aaccattctt atcattaata ttaaatacaa gatctttcag acgggaaata tattcctgag
     4261 atttatcatc aagtgaaaca ctataacgag acaatgttcc ttctgcactg ttttgaaact
     4321 tataaaacat ttccagtaat ttttttgcat aaggcgttgt atcaaagctg gtaaagtcat
     4381 aaagatattg cgcagcgtaa ccatacaaac catgaatcca gagtccttct ctcaccaaca
     4441 ctctgagata atatgcaagg ctttgaagta tcttcgtctt tcgttgtata tcaagtaaga
     4501 cacttaatga ataaaatgca ttatagaatt tatcaatatt cctgagcact ttctccagtt
     4561 catccttatc cagcttcttc tcccggtaat cctttttata ttcatttttg atagccatga
     4621 tatattgttg catcaggctc ttgcttttcc cgttatcctt ccgtatagtt cctgtatatt
     4681 tccccagagc atacagcttt ccattttcat gaaaatcttc cttgttgaat cccagttcag
     4741 ccagatattc ccgaagataa cgtttaacag cagcttcatc gctttctgta tcagcgtgat
     4801 caagacaagg acaatacgat ttctgtaaaa aattataaat atgataataa aaagcagagt
     4861 cattctctgg tctgtatgta ttatacatat ctacaacaag aaaaataacc ccaaaagtgc
     4921 gcagacaata gctgcgaagg aattcaggtt ttagtgtgga atgcactgga taagaattca
     4981 tcatgtaata aaaatgttca tatcgggcat atatgatccg ccgttgctcc tgactgaaca
     5041 ccccttcagc atcaataata cgactgatct cattataacg ttgatcccag aatttttgat
     5101 gcatttcaaa attggtactc ttccgcttgt gtgccggatt ctcgccttcc ttaaacaacc
     5161 ccaaaacagt acaaatatca tacaacatat ttgtaaatgt gcgcatatgc tgtgctctcc
     5221 ggttggtttg cattttatcg cagaggacat tctgagctta acgtatcaaa ctgacacaac
     5281 gacatgcaat gcatatggag aacaatatgt caaatcaaac cggacagctt tacacgcctt
     5341 cacgcatcct gcgtatggct gaacttaccc gactgctggg tatatcaaga tctactattt
     5401 acgtgaaaat aaatccggcc tcgaaatacc acgaccctac ctttcccaaa ccaataaggc
     5461 ttggtagtgc atccgtaggc tggcgggcat ctgccatcga tgagtggctg atgttacata
     5521 cggcaccagc ctggtctgaa cctgaaaaca aactaaagga acttaatgat gacgacaaaa
     5581 ctatcactcc ctgaacttga cacaactaaa ctgcctgaca ggtgccaggc attacttgat
     5641 gaaatgcatg aagaaacagg aatacgccgt gaaatacttc tgtctgttat gctgacggtc
     5701 atggctgcct cggtccagga tacgcatgaa gttgaacttt ccggagggca acgtaccagc
     5761 cttcagatat ttatgtgtct ttcatcagca tcaggcagtg gaaaaacctc tgcctgcgca
     5821 aaattaatcg ctcctgttca tgaaacagaa gaagaactac atcaggcata tatcgacgat
     5881 aaaaaaaact atgaccgaat gatggaaatg tggacaacag ataaaaaaat cctggagcgg
     5941 agatataaaa aggaaattga aagatccccg gaaaatgctg ctgctgcacg agcagcactt
     6001 gaaaagtgta tagcaaataa acccgaacct cccgtacaac aggttctcat cgtgaatgac
     6061 gcgacaccag aagggatagc tttgaaactc agccagtcac cctctcttct gttgctgtct
     6121 gatgaaggag gtactattct ggataagcgt ttcgaacgca aatcggcact gtacaacaca
     6181 ttatggagtg ggcaaccagt caacgttgaa cgggcatcca gacccgggtt ccggctgaat
     6241 gatgtccgtc tcactatact tatactgact caaccggtaa tatttaataa gttttttact
     6301 ctcactggcg atcagatccg gggcaatggc tttctggccc ggttgctatt ttgcgaaccc
     6361 ggagcaatta aaataatgac gacagaaccg catactgcca ctcctgttgt gacgcaacaa
     6421 tgtgcaagct gttttcgtaa gaaaagtttt ggcagtcaga tcagagactc tctgagagca
     6481 tccagagaaa gacgtgcaaa aagtaaagag cgcatctgca tgacattatc aaatgcagca
     6541 tcccgcgcac tggagcgctt tcacaaagaa aatatggata ctgtcagaca gaatcctcat
     6601 atgggtacat ttgaagacat tattgtcaga aaaagagagc aggttgtcag gatagcagca
     6661 cttctggaac tggagaaaga tccagactcg acagtaataa cgctggaaag tactaatagc
     6721 gccatttatc tcattgaatt ttatttcaag catcttatat ataaacttga atcccttcgg
     6781 gaaatatccc cggctgagaa acttgataaa tggcttcaga aaagaattat cacaacagca
     6841 ggttacatat tccagaaaag ttatatttta caatatgccc catatgctct caggaaaaaa
     6901 tgtgttctgg atgaagcact tgacatactg gcggaacaaa ggaaaataag gattgatgat
     6961 aacctggttg tttatatagg aaacacgatt acaccatccg aactggcaaa aaaactaaat
     7021 attcctgcat tcgatgccgg agttttcatt tgtgatcatc aaaacatact gaagtatcac
     7081 cgaaacagac tatagatact atcggacaaa aaataaatta tcacaagtgt attattcgtt
     7141 ataataatga ataatcgtta tctgagtgca atgccaggtt ttcattacga actggcattg
     7201 catttatttt tcaaaaaaga aagcagtaac catttggcta ctctgagtat gccagtccat
     7261 gaaactaagc gaattcctta gtgtttttag caagttgaga catcacataa tccgcccatg
     7321 cctgcatcat cggtacacgc tgctccagta gatcagtacg atgatatgcc gcctcaacct
     7381 tatttttcag cgtatgagcg agcgcccttt ccgccagatc ccgcgaatac ccctgttcac
     7441 tacaccagtc cctgaatgtt gagcgaaaac catgtgccgt ggcaactcgc cccggaatgt
     7501 cactgacggc tttcttttta cgcagaaaac ttgtcaacac catatcggaa aggatctgct
     7561 gctttctggg tgaagggaac accagttcat catgcaggcc acgtatattt tccagaatgt
     7621 aaatagcctg ccgggataaa ggaacacgat gctgtagcct agctttcatt ctttctgcag
     7681 gtatagtcca tacccgctta tgaaaatcaa tttcagccca gcgcattccc ctggcttcgc
     7741 ccgagcgagt tgctgtaagt atcaccatta ataacagtgc gcgggtaaca ttataaggtt
     7801 catcactata cacactggtc gccacaaaaa gcggtaactg cctccagggc attgcgggtt
     7861 ggtgttcatc acgtcctctt gtctgctgag gaagcaaatg gtcaaccaca tcaacaggat
     7921 ttgccacaca aaaaccgtgc gcccatcccc actgcataac aacatgaatg cgctgtttaa
     7981 cccggcttgc cgtttctgac aaggttaacc agactggacg cagtgtttct gccacgtccg
     8041 cagccgtaat cgaatccagc gtttttgctc ccagttgagg aaacgcgtaa ttctcaagcg
     8101 tcgataacca ctgccttaca tgctttggat tctcccatcc aggagacagt tctgcatgta
     8161 cacgcctggc tgcatcggca aatgttggga taacgacttt ctcagattca gcctttttaa
     8221 tctccagagg atcatcacct gcagcaagtt gctctcgcat tatccgggca gtacgtgcag
     8281 cttcagcaat actgacctct gggtaagttc ccaatccagc attacgtctt ttttgtgtca
     8341 ccggacttac ataacgaaaa acccatttcc cccgcccctt tactgaagaa ggatgaaggg
     8401 tcagtccggt aattccccca tggggcaatg gtttgtcatc aggtttgata tgtcttgctt
     8461 tcgtatccgt caatactgcc atacgctaat tcccgtcact ctggtatgcc atccagtatg
     8521 ccattagtcc ctcgcttcac taagaataca ttagataaca tcggacaaca aaaagtgtaa
     8581 tacattgttt ataaagaaat tatataacac ccctggatta agtcagattt atttcaggcg
     8641 gtccccctca ccgccatatt taaagaagag cgcgtacgaa agtacgggct tttttttcgt
     8701 atattgtaca caccgggtgc ccccgcccac gtctgttagg gcgaggggaa attgtgcaca
     8761 tcaactgtct ggttactcag tcaacaatca aaatgcgtaa gatagtacag aacatatatg
     8821 cgttatctgg tcacttcctg taacttcctc acttattaaa atgcccccaa cggaaataca
     8881 tgtcacatgc catcataaaa tcgttatgcc agaggaatcg acaatggaaa aaatagtaaa
     8941 ttaagcacgg tacatagcag agtaaaaaat aggcttcaaa ccaaatccgc cattccacaa
     9001 gcgttgccaa taccgacacc agcgtaatcc acatcatttc aataacccgc aatatgaata
     9061 accgaagcca aagcggcttg gcaaaaaagg tcgacaacgt attttcactg gagaaaaaca
     9121 gatcgatttc ctgcaaaaaa aacctgccca gaaaagcggg ccgcttggag aagcgcagaa
     9181 aaaacagatt aatcgccaac actacgccac aaagaatgag caatgcaatt atcgatttaa
     9241 acgttaacca ctgcccctct gtcacaaaag aatcactgtt acgcagcggg ctgatagtaa
     9301 gcaggttaac aaccgttgag atgcacaccg aaagcagcac atagcgagtc agacttttat
     9361 attttacttt tttcgctact gaaatagcga cctgcttgac ataactggtg cggatcatcc
     9421 gaaacagcag aaagatgacc aacggcccaa gtaacgaaat caggaacagc gcatataacg
     9481 agggtagaag ctggtatagc ccccaactca ttgcactcaa catgattaac gacagaaatc
     9541 ctgccaccag aggttgtgta agaaataatt ctattttgtg gttctgcttt ttcttaaacc
     9601 acgaaaaatc gatattcttc gtgacgcatt tataagccca gtacgccttg agatcaaaaa
     9661 taaaattata cagcaccagg gccagaatcc cactaactac accaatatcc tctgatggaa
     9721 aagtaaaccc tgcatttttc caataaaagc ctatcagaat aagtccaata gcataaatgt
     9781 atatcagatt tcgtttatcc tgaaagcgaa taaaagttaa ataatcagga atcatttgaa
     9841 tacctttgca tcactaaatg ctttacgaca atgcataact cgttgctgat ccacgccagc
     9901 aaaatcattt aacatattcc ccaaataggg cttattactt atttcaagga aagctaccgg
     9961 ggttggcata ttcagaatat gtatcgagag attcagtcca tttcaccgta tgagttaagt
    10021 gtaatgagag attttctttg ataattgacg gagcgacctc gctttttgct gttacgttca
    10081 tcagaacctg atgctctggc gaagcgatat ccagtccggc aagataatcg cgcattgcct
    10141 gaacgccgtc ttccatcaaa cgcgtgtgcc aggcaccgct cacaccgagt ttaaccggtt
    10201 cgtatccagc ggccatcagc agcgtggtaa attcattcaa cgcagcctgc gttcccccaa
    10261 taacctgctg gcgcggcgtg ttatcacagc taatatccag cgcaatgcct gattccgtaa
    10321 tcatcgtctg cagttgttcg cggttgatgc ctttaaccgc ctgatctt
//
LOCUS       DAJTUP010000125         9946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTUP010000125 DAJTUP010000000
VERSION     DAJTUP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.125"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..138
                     /locus_tag="OO468_003425"
     CDS             <1..138
                     /locus_tag="OO468_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6260411.1"
                     /translation="EIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIG
                     K"
     gene            complement(279..1052)
                     /gene="uxuR"
                     /locus_tag="OO468_003426"
     CDS             complement(279..1052)
                     /gene="uxuR"
                     /locus_tag="OO468_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR6260412.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1267..2727)
                     /locus_tag="OO468_003427"
     CDS             complement(1267..2727)
                     /locus_tag="OO468_003427"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR6260413.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(2808..3992)
                     /gene="uxuA"
                     /locus_tag="OO468_003428"
     CDS             complement(2808..3992)
                     /gene="uxuA"
                     /locus_tag="OO468_003428"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR6260414.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            4332..5675
                     /gene="gntP"
                     /locus_tag="OO468_003429"
     CDS             4332..5675
                     /gene="gntP"
                     /locus_tag="OO468_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR6260415.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5926..6828)
                     /gene="fimH"
                     /locus_tag="OO468_003430"
     CDS             complement(5926..6828)
                     /gene="fimH"
                     /locus_tag="OO468_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR6260416.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6848..7351)
                     /gene="fimG"
                     /locus_tag="OO468_003431"
     CDS             complement(6848..7351)
                     /gene="fimG"
                     /locus_tag="OO468_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR6260417.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7364..7894)
                     /gene="fimF"
                     /locus_tag="OO468_003432"
     CDS             complement(7364..7894)
                     /gene="fimF"
                     /locus_tag="OO468_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR6260418.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7904..>9946)
                     /gene="fimD"
                     /locus_tag="OO468_003433"
     CDS             complement(7904..>9946)
                     /gene="fimD"
                     /locus_tag="OO468_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR6260419.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2311 a         2623 c         2501 g         2511 t
ORIGIN      
        1 gagatccatt caaaaatggg atataacgtt atccccggtg atgaagatgc gccactcaaa
       61 cgcatgactc tgcaaccgtc atctcttcca gaactgtttg aattaaaaaa tggtgaatgg
      121 aattatattg gtaaatagcc actgagaaga atgagtacta atattcaggt tatacagata
      181 tttaaacaaa accctgcaat ttacaaagta atactcattg cgacttctta atttaagaaa
      241 gcgcaaggaa cgtttaccct tgcgcttatt ataataagtc aggcgtcgac tttatccagc
      301 ggccatgaat caaagagata gccgtcaaaa taaatatcgt caacgttcga gaattccagc
      361 agacgttgct taacgttttc cagatgctgc cacattgcca gcttcgccgc tcgggcatct
      421 tttttgatta acgcggcgag gatctgtttg tgatcgccca accactcttt gcgatacagg
      481 ctgtcatcca gatggctgtg caactggatc cacattggat tgttttcccg ccactgccag
      541 gactgacgga ataactccac cagcatgctg ttatgcgttg cttcagcaat ggcgagatgg
      601 aactgcatgt caccgctttc gctgctaccc ggcgcgctgg aagccagttc acgctcttcc
      661 agttgcaatg cctgacgcat tttgacgata tcttcgcggg tagcctgcag tgcggcaaac
      721 tcggcgatgt tactttccag taactgacgc gcctgtaaca gctcaaaagg acccgcatca
      781 ttgcagacgt tggcatccgg actgtctgtg ttgtggctgc ctgagctatc aagcacatag
      841 ataccggcac cccggcgcac ttccaccagc cctttgatct ccagcatgat cagcgcttca
      901 cgtaccaccg tccgcgtgac atcaagcatt tctgcaattt cacgctccgg cggcagccgt
      961 tcgccaggat tgtacggcgt ctttatgatc agatcgtgga tcatcgcccc gacttcctgg
     1021 taaggtcttt gcgcagaggt gacagatttc atggtgttgt gtccgttagg tgcggttaat
     1081 caaatttttg ccggatgcgg cgtgaacgcc ttatccggcc tacaacgggc aattttacgg
     1141 cccccgttaa tctcaaacta taccattagc actggaagtt ggcacgtaca ggtgggcaaa
     1201 aaaacgaaaa ctggtcaacc aaatatcagt ttgattgacc agtaagtctg tagggtaatc
     1261 agttagttac agcgcagcca cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc
     1321 cgacgtcacc gcgccaacaa agtcggcatt ctgcggcaga tcatcggcaa aaataccgct
     1381 caggcccagc agcgctttca cgcggtctgc gccctgatac tgcgcgttaa tcttctggaa
     1441 ctcggccagc atcgggtcga ccacgtcaat ggcattaccc tgctcatcca cgccctgggt
     1501 gtaacccatc cagccagcca cgcccagcgc caggtgacgc cagctgccgc cgttttgcag
     1561 gtgcagacgc accgggtcca gtagacgctg cggtaacttc tggctgccgt ccatcgcaat
     1621 ctgccaggta cggtgacgca gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata
     1681 ggcgttcagg tccgtacctt ccggcatcga cagcgttggc gcttgttcct gcatcatcag
     1741 ggcaaaggcc gctttgcgat aatccgggtt agtcatggtg tcggcaatgg tttcatagcc
     1801 gccgaggtaa ccgaggtacg ccaggaaaga gtggctgccg ttcagcatac gcagcttcat
     1861 catttcgaac ggcacaacgt ctgcaacgaa ctgtgtgccc actttatccc agtccgggcg
     1921 accattaacg aagttatctt caatcaccca ctgacggaac ggttcgcagg caatggcgca
     1981 cgggtcgtaa acacccagct ggtcagcaat ttcctgtaag gtttctggcg tcgccgccgg
     2041 aacgatgcgg tcaaccatgg tgcacgggaa ggtgacgttc tcttcaatcc atgccgctag
     2101 ctgcgggtca cgcgcctgag ccagccccag taccgcgacc ttcgccacat ggccgttttc
     2161 acgcacgtta tcgcaggaca tcaccgtaaa tgctttcagc cctttttcac gacgcagacg
     2221 caaggcttcg acgatgtaac caatcgcgga cttcggcgca gtcgggtttt ccagatcgtg
     2281 cttgatcagc gggttattga gatccagttg accgcttgcc gcatcagcgc agtagccttt
     2341 ttccgtgacc gttagagaga caatcgccgt ttgcggacgc gccatcgcgt tgagaatacc
     2401 ttcgcagccg tcgatttccg gatgcagcgc ttctttcatc gaaccgataa ttttcagctc
     2461 ggtgctttct gcgccttttt ccgctacggt gtacagcagt tgctgttttt tcaggttttc
     2521 gatcagcatg cggtcgttgc ctggcatcag gttaacttcg cagatgcccc agtcgctgtc
     2581 ggtgctttcc agcagatgat gggtatacag cgcctggtgc gcgcggtgaa acgccccgca
     2641 accgagatgc acaatgcgtg attccagacg agaatgatcc catgacgggc gggcaaccgg
     2701 cagattgctg tcaacaatag tagtcatcgt aaactccaga atccgtcttc agagagtgac
     2761 ggcaaggaag aaggaaccgg cacggcgcgc agccatgccg gtgggtatta acggataaag
     2821 aaagcgcgct ggatcgccag ttcgacaccg cgaacttcgg ccaggccttt cagacgacca
     2881 attgcggagt aaccagggtt ggttttcttc ttcaggtcgt ccagcatctg atgaccgtgg
     2941 tccggacgca tcgggatcag gtcttctttg ccttccgctt tacgacggtg ttcttcttca
     3001 acaatcgctt tcaccacttt gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg
     3061 aaggttttcg ggttatcttc acgcatggtg gagcgcagat gggtgaagta aatacgcgga
     3121 ccgaactgct tgatcatatc aaccagatcg ttgtcagcac gcacgccgta ggaaccggtg
     3181 cacatggtga aaccgttcgc catgctgttt acggtatcaa ccatccactg catatcttca
     3241 atggtggaaa caatgcgcgg caagccgagg atcgggcgcg gcggatcgtc cgggtgaacc
     3301 gccatacgca cgccaacttc ttcagcaact ggaataatcg ctttcaggaa gacggcaaag
     3361 ttttcgcgca gtttggcttt gtcgatatct ttgtacagct ccaggtgctt acggaactgg
     3421 tcgagggtat acccttcttc cgcgcccggc agaccggcaa tgatgttacg cgtcagacgc
     3481 gctttgtctt cgtcgctcat agtggcgaag cgttcagctg cctgagcaat ttcttcttcg
     3541 gtgtaatccg cttccgcgcc tggacgtttc aggatatgca tttcgaatgc agcgaattcg
     3601 atctggtcga agcgcagagc tttggagccg tctggcagca cgtattcgag gtcagtacgg
     3661 gtccagtcga gcaccggcat gaagttgtag cacacggtgc gaatgccaca ctgcgccagg
     3721 ttgcgcagag tctgctgata gttagcgatc cactgctcat agttgccagt gtgggttttg
     3781 atatcttcgt gaattggtac gctttctacg acagaccaca ccaggcctgc gtcttcaacg
     3841 atcgccttgc gtttgaggat ctcttctacg gaccatactt cgccgttcgg gatatggtgc
     3901 agcgcggtaa ccacgccagt tgcgcccgcc tgacggacat cagctaaaga aaccggatcg
     3961 tttgggccgt accagcgcca ggtctgttcc atgatttttc ctctttcaca tagtgggttc
     4021 cacagcaaag agacgcggaa ccttggagtt gtttaggtat ccggatgaac aaccagaccg
     4081 cagccgtggc gttgcatcgg atgagatggc gtataagttc taccttttgg taagacatgt
     4141 caaccagaat tctgaaattg gttaaccgcg tcgcaagaat ttcactctca tggttgttgc
     4201 tgcaaattgc agcaaagggt aatcgatggt tattgatcag aggatgtgac attcatcgca
     4261 acaatggttg accaatttac ataacatgtc gcccaaaata acaacggttc aaccaagtaa
     4321 ggtaaaacgt tatgcatgtg cttaacattc tctgggtggt attcggcatt ggcctgatgc
     4381 tgatactgaa tttgaagttc aaaatcaatt caatggtggc tttgttggtg gcggcgctgt
     4441 ccgtcgggat gctggcaggc atggatttga tgtcgctgct gcacaccatg aaagcgggct
     4501 tcggcaacac gctgggagaa ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat
     4561 tgatggtcga ctccggcgcg gctcaccaga tagcacatac gctgctggcg cgtctcggtc
     4621 tgcgctatgt acagctgtcg gtgattatca tcggccttat tttcggtctg gcgatgttct
     4681 atgaagtggc ctttatcatg ttagcgccgc tggttattgt tattgccgcc gaagctaaaa
     4741 ttccgttcct gaaactggcg atcccggcag tagcagctgc cactaccgca cattcactgt
     4801 tcccaccgca gccgggtccg gtggcgctgg tgaatgctta tggcgcggat atggggatgg
     4861 tttatatcta tggcgtactg gtgacgatcc caagtgtaat ctgcgcaggt ctgatcctgc
     4921 cgaagttcct cggcaatctt gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg
     4981 tagatatgaa taatctgccc tctttcggcg tttcgattct ggtgccgctg atcccggcga
     5041 tcattatgat ctctaccacc atcgccaata tctggctggt aaaagatacc cctgcctggg
     5101 aagtggttaa ctttattggt tcctcgccga ttgcgatgtt tattgcgatg gtggttgcat
     5161 tcgtactctt tggcaccgcg cgtggtcatg acatgcagtg ggtgatgaac gcttttgaaa
     5221 gcgcggtgaa gagtattgca atggtgattc tgatcatcgg tgcgggtggc gtgctgaagc
     5281 agaccatcat cgacaccggc attggcgaca ccatcggcat gttgatgtcc cacggcaata
     5341 tctcgcccta cattatggca tggctgatca ctgtgctaat tcgtctggcg acgggtcagg
     5401 gtgtcgtttc ggcgatgacc gccgccggga ttatcagtgc tgcaatcctt gatccagcaa
     5461 ccggtcagct ggttggcgtg aatccggcgc tgctggtact ggcgacggct gcgggttcca
     5521 acaccctcac ccacattaat gatgcatctt tctggttgtt caaaggttac tttgacctgt
     5581 cagtaaaaga cacgttgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc
     5641 tgattattgt gctgattatt agcatggtag cgtaaaagaa caccagagcc tgccaatggc
     5701 aggctcagac tgatgactaa agtctaaaaa tgtgcccgga cagtcccctc gcccctccgg
     5761 ggagagggtt agggtgaggg gaacagacca gtaaagtgcg ctttgtttat gccagatgcg
     5821 acgctgacgc gtcttatctg gcctacaaag ggctaacgtg caggttttgt aggtctgata
     5881 gcgtagcgcc tcaggtacca gcattagcaa tgcgctgtaa tttttttatt gataaacaaa
     5941 agtcacgcca ataatcgatt gcacattccc tgcagtcacc tgccctccgg tacgtgcgta
     6001 atttgccgtt aatcccagac ttaccgccga agttcctact gctcctaacg ataccgtgtt
     6061 attcgctgga ataatcgtac cgttgcgcgt caactgtacg ccgacgccct gcgctggtga
     6121 aaacgacgcg gtattggtga aaatcgagtt gcccgcatct gcggttgtgc cggagaggta
     6181 ataccccagg ttttggcttt tcgcacaata aacggtaaga ggaattggca ctgaaccagg
     6241 gtagtccggc agagtaacgg tgacatcacg agcagaaaca tcacaaccgc cagtgggcac
     6301 caccacatca ttattggcgt aaatattcca cacaaactga aaatcatcgc tgttatagtt
     6361 gttggtctgt cgcaaaataa gcacggcaat taatgaacca gctttaatag ccaccccgcc
     6421 cgcactgctc acaggcgtca aataaagcgc caccggccac ggcttatccg ttctcgaatt
     6481 ataaacaacc cgcggcgttt cgctggtggt cggaaatgga tagctactgc cactatattt
     6541 tacggtcccg gaaaaattag ataacacgcc gccgtaagcc gagcctcgtt gcagtgtgac
     6601 atagtctgta atggtttccg gataatcgtt atggcaaaag atttgcgtcg aaagatctac
     6661 gaccaggttt tgccccacat tcacgacagg cgcaaggttt acataaacat tagcgctgcc
     6721 accgccaata gggatagcgg taccattggc ggttttacag gcgaatgacc aggcatttac
     6781 cgaccagccc atcagcagta cagcaaacag ggtaatagct cgtttcatta caattatctc
     6841 ttcgggttca gctgtaggta taggtgatgc taatcactgc ctgaatggtt ccctgagtgg
     6901 ctccgccatt taccgtcaat gctctgacct gtaacgggaa gtgcgctgat tgtgaggaat
     6961 catccacctg aactgttttg gttgcgccag tattcaatgt gttgccactg tcatcctgta
     7021 gctctaactg gatgttttgc gcggtcccct ggtttttata atatccggtg ctgtcggctg
     7081 ccccgctgaa gctggcagtg accctcgacg ttcccaccgg acaattagtc aactcaagcg
     7141 caacatcatg ccaggccgat gccgccccgg cagacataag actgaaagaa taaagatcgc
     7201 cgagatctac cgtggcattg gtggtggaaa ccgtacacgg tttggcgacg accttaccgt
     7261 tcaccgtgat ggtgacatcg gctgcctgta tcgtcgcact tgcgagcgcc aacattgccg
     7321 ccaatacata cccacgtttg caccatttca tgagcatctc cagttactga tattcaagag
     7381 tgaaggtagc cgtagcattg atatgccccg cagtgacagg cacctgtgtc gccattagcc
     7441 gggcgtaaaa attcagcgta tttggtttac ccggcgtcag ggtcgtccac gaaatcgcag
     7501 acgatggagc attaaggggt atttgatttt gctgctcatt cagaagctgt attcccagtc
     7561 ccgcagccgc tgacaccgta ttttcaagtg caagcaggtt ggcattgtgg ctatctgcaa
     7621 cgccggtaaa cccaactttt acggcagaaa cggcattacc acagggtgac agcaaaatac
     7681 gaaatggaac gacaggagtc gtcgcgccaa tgttgttaaa ttgcttcgcc gcgttttcca
     7741 tcagatcaac agtaaaattg gttgattcag cggccacact acagccgtta tctctgacat
     7801 agccgcggat agtaatcgtg ctatccgcag ccaaaacgcg acttaccgcc agccacaaaa
     7861 aagcgcacag aagataaaaa ggtttgtttc tcatcacgcc cccttaacga cattcagctg
     7921 atagctgggt taataactgc tgctgactct ctggtggcag ttgataattg gcgacacagt
     7981 gagcattttc ctcttctccc catttcacct gaacttttcc cgctaaaggc attccgctga
     8041 ggtaaacctg accattatcc gcaacaatgc cgctactctg gctactctct gatgtcacca
     8101 tcgccccaaa cggcagcggc ttattattgt gagttagcgt catgagcagt tttatcccaa
     8161 cgcgcgcttt aaactctgct cgcacgatcg ccccacgagt gggaacaacg ttagcgaccg
     8221 cgttatctaa atcgacgtta tcagccaagg tattggtatc cagcgccact ctattttccc
     8281 gatattcagt ggcataaggc agcacggcat aaccacgcca gtcggtacgc acccccgtct
     8341 ggttttcgac ttttgcatct tttgcgccag gcgctttaac aagcaccacc gtatcgttta
     8401 acggctgccc cagcgttacg ccattggcat gagccaatac cccaccgctg actccgtaat
     8461 agagctgctt aatatcatcg ctatggctgt aaccgatatt ggcattgccg taaccaccgc
     8521 gataattcag cgtggcgtag cctgtacttc cgctattacc atcgcctccc ccggcatagc
     8581 cggtttgcac gctataactg aggttgttgt cttccagcaa cgtaccgtat acaccagcca
     8641 gattggtcat ccgaccgttg agatcgtgtg acatgctgta gctggcactg gcatgtcgcc
     8701 actgagattt actgtcagaa cgcagccagt ggctgaaagg aatattgacg ttaagcgcta
     8761 acatctgatc ccgtcctttt tgccaggcgt ttttcgtcag gctatagctg agcgtccagt
     8821 tgatatcttc gaacgcagta tttaatccag cctggaattg ctcatcgaca ttactcgttc
     8881 cccaataagt ttgatggcta ccactcaaat acagtgttga tgtgcgcccg agttgctgag
     8941 taacggtgag ctgtaatttc ccgcgtttgt tataagcgag gttgtaatag tcggtgaatt
     9001 tcggcttaac ctgaataact ccatcctgtg tttcgatgtt gtagccattc attcgactgt
     9061 atgttgtatc agcgaaatta aaatatccgc tggtcgaata acggtaaccc actaactgaa
     9121 tattcgtgcc tgattcattg agcgatttgt tatagagaaa acgcaccgat tgtccgtcat
     9181 gctgactgtc atcgggaagt gtggaattag cctgcgtcat atccacagac agagcgccca
     9241 gtgcctccat gtttttcccg ataccgaaat taaaagcacg ataacgatcc gccagttgcg
     9301 ttccaccata tattgtccag ccagccggaa ggccgtggag taatgtactc tggaaaaagc
     9361 ggggtttttc ctgctgcgca tttccactac ggtattctcc tgccgtaatg gaataacgag
     9421 tatgcccttc acgttgcaaa agcgggactg acgaataggg tacggtaaaa atctgcgtgc
     9481 tgccgtcagc ctctttgatc gttacctgca agtcaccact attacctgcg gcatagatat
     9541 cgttgatggt aaaaggcccc ggtggcaccg tactattata aatgccatat ccattttgtt
     9601 taatagtgac ctgtgcagta ccacgagcaa taccgtggat caccggggca aatcctcttt
     9661 ggctatcggg taacatattg tcatctgagg ccaattgtgc gccgcgaaag ttaataccat
     9721 cgaaaatatc gccctgggta taaccatcac ccaacgtcag ccgggaacgt aaaggaatta
     9781 tgtctcgctc aatccaggta atgatatgct gccatttatt tttgctacct gatgaactgt
     9841 cgctactgtt ataactccag gtggtattgt cgcgtaaacg ccacgcacca atatttaacc
     9901 cactctgtag gtttaaatat gcataatggc tgttaccccc aatccg
//
LOCUS       DAJTUP010000126         9889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTUP010000126 DAJTUP010000000
VERSION     DAJTUP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.126"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            323..1318
                     /gene="pgl"
                     /locus_tag="OO468_003434"
     CDS             323..1318
                     /gene="pgl"
                     /locus_tag="OO468_003434"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR6260420.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1359..2312)
                     /locus_tag="OO468_003435"
                     /pseudo
     CDS             complement(1359..2312)
                     /locus_tag="OO468_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2496..3548
                     /locus_tag="OO468_003436"
     CDS             2496..3548
                     /locus_tag="OO468_003436"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR6260421.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3624..5057
                     /locus_tag="OO468_003437"
     CDS             3624..5057
                     /locus_tag="OO468_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR6260422.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5240..7420
                     /locus_tag="OO468_003438"
     CDS             5240..7420
                     /locus_tag="OO468_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR6260423.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVEPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7561..8844)
                     /locus_tag="OO468_003439"
     CDS             complement(7561..8844)
                     /locus_tag="OO468_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR6260424.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(8979..9860)
                     /locus_tag="OO468_003440"
     CDS             complement(8979..9860)
                     /locus_tag="OO468_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6260425.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2394 a         2312 c         2512 g         2671 t
ORIGIN      
        1 tgtgccggaa gtgagttgat tgttgcgcga atgggaagtt ggccagaagt tgttatggca
       61 tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg agagtcatac tccctgaata
      121 gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt gactaatatt ttagtaatat
      181 gccattcgaa atggagtgct aacttattga tttaattgat gtgcgatatt gtattttatc
      241 gtgttttgtt gtctgttttt tatccagtaa gtgttgttat atgtatgaaa gataagggat
      301 ttatcatttt tgaggtgtgt tcatgaagca aacagtttat atcgccagcc ctgagagcca
      361 gcaaattcac gtctggaacc tgaatcatga aggcgcactg acgctgacac aggttgtcga
      421 tgtgccgggg caggtgcagc cgatggtggt cagcccggac aaacgttatc tctatgttgg
      481 tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc ccggacgatg gcgcactgac
      541 ctttgccgca gagtctgtgc tgccgggtag tccgacgcat atttccaccg atcaccaggg
      601 gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg agcgtaacgc gtctggaaga
      661 tggcctgcca gtgggcgtcg tcgatgtagt cgaggggctg gacggttgcc attccgccaa
      721 tatctcaccg gacaaccgta cgctgtgggt tccggcatta aagcaggatc gcatttgcct
      781 gtttacggtc agcgatgatg gtcatctggt ggcgcaggac cctgcggaag tgactaccgt
      841 tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac gaacaatatg cgtattgcgt
      901 caatgagtta aacagctcag tggatgtctg ggaactgaaa gatccgcacg gcaatatcga
      961 atgtgtccag acgctggata tgatgccgga aaacttctcc gacacccgtt gggcggctga
     1021 tattcatatc accccggatg gtcgccattt atacgcctgc gaccgtaccg ccagtctgat
     1081 taccgttttc agcgtttcgg aagatggcag cgtgttgagt aaagaaggct tccagccaac
     1141 ggaaacccag ccgcgcggct tcaatgttga ttacagcggc aagtatctga ttgccgctgg
     1201 gcaaaaatct caccacatct cggtatacga aattgttggc gagcaggggc tactgcatga
     1261 aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg gtggttaacg cacactaacc
     1321 gctgatttaa ccggcgcagt ctctcctgcg ccggtgtatt aacttatctc ctgtaacgca
     1381 tgtctctggc gttcgacgat attggtccac aaattgtctt tatcgtcagt ccacaaatta
     1441 atcagcaagg aaaaaaagcg ttcagctgcc ggagaaagca cggcatcttt gcggcgaata
     1501 atccccaatg tccgacgtat aacgggttcc accagtggga taccaataat cgaagaatag
     1561 ggcgcatggg gcattgccag accaggaagc gccgaaatcc ccagtcctgc ctccactagt
     1621 cctaatgacg tcgaaagatg acgcacttcg taaaaccagt ccagcttcca gggcttgtcg
     1681 gctagctgtt gttctatcaa caatcggttg ccgctggagg aacgtacgcc aatcattttg
     1741 taacccacta actcctgcca ttctacaagc tactttttgg ccaatggatg gtcacgccga
     1801 caggccagca cgaatggctc gttgaccagt ggggtaaaat caatggatga atttgtgacg
     1861 ttgttcatgt ttatgccaaa gtctgattcg ttacacagca ccgattccat gcaattattc
     1921 gtgccttgtt ccagaatccg cactttaata ttgggataca gctcattaaa tttaccgatt
     1981 gccagcggta aaaaataaaa tactgcggtc ggaatacacg ctaatgtcac cataccacga
     2041 tgataagcat tcatatcacg aatattaaaa agcgtttcat cgaatttctt tattaattcc
     2101 cgcgcctccg ggagcaacct ttttcctgct tttgttaacg taaccttgcg agttgtacgc
     2161 tcaaatagtt gaatatgtaa atcttcctcc atctttttta ttcttcgcgt taatgcaggt
     2221 tgcgtaatat tgagtaattt agcggcattg ttaaatgaac tggactctgc cagtatgaca
     2281 aatgccttca tacttgataa ttcatgcttc attattactc cggaaaatgg aagcgacgat
     2341 tttggggggc tggtcgttaa aaattttaac tgcatttagc caacttaaat taatgaaaaa
     2401 atgttattaa tcgttgagct aaagtcatta gagatgcttt gtccttaatg taaccatatc
     2461 gcaataagtt atgtttttaa attgagggca ttattatgaa aaaaataccc tgcgtgatga
     2521 tgcgaggtgg aacctcgagg ggcgcgttcc tgttagcgga acatttaccc gaagatcaaa
     2581 cgcagcgcga taaaatattg atggcaatta tgggttccgg taacgatctg gaaattgacg
     2641 gtattggcgg cggtaatcca ctgaccagta aagtcgccat tattagccgt tccagcgatc
     2701 cgcgtgctga tgtcgattat ctgtttgctc aagttatcgt ccatgagcaa cgtgtcgata
     2761 ccacgcctaa ctgcggcaat atgctgtctg gcgtaggggc attcgccatt gaaaatggtt
     2821 tgattgcagc gacttcgcca gttacccgtg tacgtatccg caacgtcaat acgggtacgt
     2881 tcatcgaagc tgatgtgcaa acgccaaatg gtgttgtcga gtacgagggt agcgccagaa
     2941 ttgacggcgt accgggtact gccgcaccgg ttgcgctcac tttcctgaat gccgctggaa
     3001 cgaaaactgg aaaagttttc ccgactgata atcagattga ttattttgac gatgtcccgg
     3061 tgacctgtat cgatatggcg atgccagtcg tcattattcc ggctgaatat ctgggaaaaa
     3121 caggttatga attaccggcg gaactggatg ccgacaaagc attattagcc cgcattgaat
     3181 ctatccggct acaagcgggt aaagcaatgg gcttaggtga tgtcagtaat atggttatcc
     3241 ctaaacctgt gcttatttct ccagcgcaga aaggcggggc aattaatgtg cgttatttta
     3301 tgccgcattc ttgccatcgc gcgctggcga taaccggtgc tattgctatt tccagtagtt
     3361 gtgcattgga aggcaccgtc acccgacaaa tcgtcccttc tgtaggatat ggcaatatca
     3421 atattgaaca ccccagtggt gcgctcgacg ttcatttaag taatgaaggt caggatgcca
     3481 cgacgttacg cgcatctgtt attcggacaa ccagaaaaat attttccggt gaagtttatc
     3541 ttccctgaaa aaattcgttg tcaggataag gacaatcaat aaaggacttc tgtatgagtc
     3601 atacagaaag aacaggattt taaatgaata agaaatcgtt atggaagcta attctgatat
     3661 tagcgatccc atgtattatt ggttttatgc cagctccggc aggattaagc gaactggcgt
     3721 gggtgctttt tggtatttac ctggcggcca ttgtgggtct ggttatcaag cctttcccgg
     3781 aacctgtcgt actgttaatt gccgttgctg cctcaatggt ggtggtcggt aacttatccg
     3841 acggtgcgtt taagaccacc gccgtattaa gcggttactc ttcaggtact acctggctgg
     3901 tgttctcggc gtttacctta agcgccgcat ttgtgaccac cggtttaggt aaacttattg
     3961 cctatctgct gattggtaaa atcggtaaca ccatgctggg tctgggttac gttacggtat
     4021 tcctcgatct ggtactggct ccggcaacac cgtctaacac cgcgcgtgcg ggcggcattg
     4081 tgttaccgat catcaacagc gtggcggtgg ctttggggtc cgaaccggaa aaaagtccgc
     4141 gtcgtgtcgg acattacctg atgatgtcca tttacatggt caccaaaacc accagctata
     4201 tgttctttac cgcaatggcg gggaacattc tggcgctgaa aatgatcaac gacattctgc
     4261 acctgcaaat tagctggggt ggatgggcgc tggccgccgg attgccgggc atcattatgc
     4321 tgctggtcac cccgctggta atttacacca tgtatccacc agaaattaaa aaggtggata
     4381 acaaaaccat cgctaaagcg ggccttgccg aactgggacc gatgaaaatc cgcgaaaaaa
     4441 tgctgctcgg tgtctttgtg ctggcgctac tgggctggat tttcagtaag tctctggggg
     4501 ttgatgaatc cactgtggca atcgttgtta tggcgaccat gctgctgctg ggtatcgtta
     4561 cctgggaaga cgtggttaaa aataaaggcg gctggaatac cttaatctgg tacggcggta
     4621 ttatcggctt aagctcctta ttatcgaaag ttaaattttt cgaatggtta gctgaagtct
     4681 ttaaaaataa cctggcattt gatggtcacg gtaacgttgc tttcttcgtt attattttcc
     4741 tcagcatcat cgtgcgttat ttcttcgctt ccggtagtgc ctatatcgtt gctatgttac
     4801 ctgtatttgc catgctggcg aacgtctccg gcgcaccgtt aatgttaacc gcgctggcac
     4861 tgttgttctc caactcctat ggcggcatgg ttactcacta tggcggcgcg gcaggtccgg
     4921 tcatctttgg cgtgggttac aacgatatta aatcctggtg gttggtcggt gcggtactga
     4981 cgatattaac cttcttggtg catatcaccc tcggcgtgtg gtggtggaat atgctgatcg
     5041 gctggaacat gctgtaaata tactcgtcat acttcaagtt gcatgtgctg cgtctgcgtt
     5101 cgctcacccc agtcacttac ttatgtaagc tcctggggat tcactcactt gtcgccttcc
     5161 tgcaactcga attatttaga gtatatccat ttattatctt tctgcgcact tcacggtgcg
     5221 cagatatctg gagcatttga tgatcaagtt atctgaaaaa ggcgtgtttc tcgccagtaa
     5281 taacgaaata attgccgaag aacatttcac cggcgaaatt aaaaaagaag aagcaaaaaa
     5341 aggcactatt gcctggtcta ttctctcttc tcataatacg tccggaaata tggataaact
     5401 taaaattaag tttgattcat tagcctctca cgatattacc tttgttggta ttgtacagac
     5461 cgctaaagcg tccggaatgg aacgtttccc gctgccgtat gtgctgacca actgccataa
     5521 ctcactctgc gccgtcggcg gcaccattaa cggtgatgac catgtttttg gtttatcggc
     5581 agctcagcgt tatggcggta tttttgtgcc tccgcatatt gcggtcatcc atcaatatat
     5641 gcgtgagatg atggctggcg ggggcaaaat gatcctcggg tcagacagcc acacccgtta
     5701 cggtgcatta gggacaatgg cagtcggtga gggtggcggt gagttggtaa aacagctgct
     5761 taatgacacc tgggatatcg actatccggg cgtggttgcg gtgcatctga ccggaaaacc
     5821 tgcgccgtat gtggagccac aggatgtggc gctggctatc attggcgcgg tgttcaaaaa
     5881 cggttacgtc aaaaacaaag tcatggagtt cgttggaccg ggcgttagcg cgctctctac
     5941 cgatttccgt aacagcgttg acgtgatgac cacggaaacc acctgtttaa gctccgtctg
     6001 gcaaactgat gaagaagtgc ataactggct ggcgctgcac ggtcgcggtc aggattactg
     6061 ccagcttaac cctcaaccga tggcgtacta cgatggctgc atcagcgttg atttaagcgc
     6121 catcaaacca atgattgcgc tgccgttcca cccgagcaac gtgtatgaaa tcgacacact
     6181 gaaccagaac ctgaccgaca ttctgcgtga gattgaaatt gagtccgaac gcgtggcgca
     6241 cggtaaagcc aaactctcgc tgctggataa agtggaaaat ggtcgcctga aagtgcagca
     6301 ggggattatc gcgggctgtt ctggcggtaa ctacgaaaac gtcatcgcgg cggcgaatgc
     6361 actgcgcggt caatcctgtg gcaatgacac cttctcgctg gcggtttacc cgtcatcaca
     6421 gccggtgttt atggatctcg ccaaaaaagg tgtggtagca gatttgattg gcgcaggcgc
     6481 aatcatcaga accgcattct gtggcccatg ctttggcgcg ggcgatacgc caatcaacaa
     6541 cggtttgagt attcgccaca ccacgcgtaa cttcccgaac cgcgaaggct ctaagccagc
     6601 caatgggcag atgtcagcgg tggcgttgat ggacgctcgt tctatcgctg cgactgcggc
     6661 aaacggtggc tatttaacct ctgccagcga acttgattgc tgggacaacg tgccggagta
     6721 cgccttcgat gtaacgccgt ataaaaaccg tgtttatcag ggctttgtga aaggggcaac
     6781 tcagcaaccg ctgatttacg gaccgaacat taaagactgg ccggaattgg gtgcgctgac
     6841 tgacaatatc gtcctgaaag tgtgctcgaa gatcctcgac gaagtcacca ccaccgacga
     6901 actgattcct tccggtgaaa cctcttctta tcgttcaaat ccgattggtc tggcggggaa
     6961 tgtcagcgag ctgacagagg tgtttgcgcg cattaagcag attgctggtc aggagcatat
     7021 tgatccgctg caaactgaaa ttggtagcat ggtctatgcg gtgaaaccag gcgatggttc
     7081 tgcgcgtgaa caggcggcga gctgccagcg tgtgattggc ggtctggcga atattgccga
     7141 ggagtacgcg actaaacgct accgttctaa cgtcatcaac tgggggatgt taccgctgca
     7201 gatggcggaa gtgccaacct ttgaagtggg ggattacatt tacatccctg gcattaaagc
     7261 ggcgctggat aatccgggta cgacgtttaa aggttatgtg atccatgaag atgcgccggt
     7321 aacggaaatt acgctttaca tggaaagcct gacggctgaa gagcgcgaga ttatcaaggc
     7381 gggtagtttg attaacttca ataaaaaccg tcagatgtaa aaagcgccat gtgaatgtag
     7441 gtcgcattcg gcacttattg tcggatgcga tgctggcgca tcttatccga cctacaaatc
     7501 gcatcgaacc gtaggccgga taaggcgttt acgccgcatc cggcaaatag ttaatagctc
     7561 ttacttcttc tcctctgcaa ccactttgct gcccacgccg cggttattgt attcccacat
     7621 gcggttgtag ttagtgtcat tcagattgcg ctgtatttcg tcgttatcat ctacgctgcc
     7681 ggtattaccc gcaaacggac gattagagat caccgcatcg gcccacggtt tagccgtgtt
     7741 aaaaccttcg ttgatggcgc tatcacggat caccacctga ccgttggtat tagcatcaac
     7801 atccagcgag cggcccagtt gcgccacgcc atcaccggaa gcattgaaac ggctgtttac
     7861 ggcgaggaaa ccgtaataga tgttagacag cgtagccggt gcaaacacat atgcttcttg
     7921 ctgggtacgt gagttcacca cgcggaattc ggtgttatcg aacaccactg cgccgcgacc
     7981 agaaacgata tccacatccc cttcaatata gctgttggtc accagcgtac gcggctgacg
     8041 gttggtttcc agacggttct gcacaccgct gttggtgaca aagaaggtgt tctgacgacc
     8101 gagaatgtta acgttgttaa tctgtacttg gtcaccgtca gtacgcagtg ctaccgccgg
     8161 atggttacct gcatctacgc tatcgcccag cgtgttttcg atggtcagat tttgcagttg
     8221 cagtccattg ttttgtgacc agaagaccgc agaacacaga acaccgatac tgtcgctgcg
     8281 tttgctctgg cagctatcgt acatatacca cgctggttta cctggcatat atttgccgcg
     8341 cgggttgacg tcgtgacgcc agtcggcagg gctcatgccc ccatcaaggg aaagcccaat
     8401 cttcacatca atcggttttt cacctgtacc gtacagagtg attccacctg gagcagcagg
     8461 gacatacacc gttccctgat actcaccagg catcacggca atatactggc gcttgttggt
     8521 gcgcttgata attgccgcat ctaccgccgc ctgaatcgtg gtatgcgtta caccttgagt
     8581 acccgccggg ccgacaacaa agtcaggttg cgcaggcagg gttatcgggg acggattcca
     8641 cgctgcagca cctggtgtca gggatgcaaa atagtgttgc gcatcgaaat tcagcgcttc
     8701 ttttgccgac agaatcgggc gagaagaggt accaggcgca gtttgatcag aaggacgttg
     8761 atcgggtggt gttgagctac aggcggtcag cgtcacgcca aaagccaatg ccagcgccag
     8821 acgggaaact gaaaatgtgt tcacaggttg ctccgggcta tgaaatagaa aaatgaatcc
     8881 gttgaagcct gcttttttat actaagttgg cattataaaa aagcattgct tatcaatttg
     8941 ttgcaacgaa caggtcatta tcagtcaaaa taaaatcatt atttgatttc aattttgtcc
     9001 cactccctgc ctctgtcatc acgatactgt gatgccatgg tgtccgactt gtgcccgaga
     9061 agatgttgag caaacttatc gcttatctgc ttctcataga gtcttgcaga caaactgcgc
     9121 aactcgtgaa aggtaggtgg atccccttcg aaggaaagac ctgatgcttt tcgtgcgcgc
     9181 ataaaatacc ttgatactgt gccggatgaa agcggttcac gacgagtaga tgcaattatg
     9241 gtttctccgc caagaatctc tttgcattta tcaagtgttt ccttcattga tatcccgaga
     9301 gcatcaacat gcaatgttgt agggatggca atttttacgc ctgttttgct ttgctcgaca
     9361 taaagatatc catctacgat atcagaccac ttcatttcgc ataaatcacc aactcgttgc
     9421 ccggtaacaa cagccagttc cattgcaagt ctgagccaac atggtgatga ttctgctgct
     9481 tgataaattt tcaggtattc gtcagccgta agtcttgatc tccttacctc tgattttgct
     9541 gcgcgagtgg cagcgaccgg gtttgttgtt atatggcctt cagctattgc ctctcggaat
     9601 gcatcgctca gtgttgatct gattaacttg gctgatgccg ccttgccctc gtctatgtat
     9661 ccattgagca ttgccgcaat ttcttttgtg gtgatgtctt caagtggagc atcaggcagc
     9721 cccctcctta ttgctttaat tttgctcatg taatttatga gtgtcttctg cttgattcct
     9781 ctgctggcga ggattttttc gtagcgatca agccatgaat gtaacgtaac agaattatca
     9841 ctgttgattc tcgctgtcag aggcttgtgt ttgtgtcctg aaaataact
//
LOCUS       DAJTUP010000127         9586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTUP010000127 DAJTUP010000000
VERSION     DAJTUP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.127"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            383..2014
                     /gene="oppA"
                     /locus_tag="OO468_003441"
     CDS             383..2014
                     /gene="oppA"
                     /locus_tag="OO468_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR6260426.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2100..3020
                     /gene="oppB"
                     /locus_tag="OO468_003442"
     CDS             2100..3020
                     /gene="oppB"
                     /locus_tag="OO468_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR6260427.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3035..3943
                     /gene="oppC"
                     /locus_tag="OO468_003443"
     CDS             3035..3943
                     /gene="oppC"
                     /locus_tag="OO468_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR6260428.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3955..4968
                     /gene="oppD"
                     /locus_tag="OO468_003444"
     CDS             3955..4968
                     /gene="oppD"
                     /locus_tag="OO468_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR6260429.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4965..5969
                     /gene="oppF"
                     /locus_tag="OO468_003445"
     CDS             4965..5969
                     /gene="oppF"
                     /locus_tag="OO468_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR6260430.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6022..6351)
                     /locus_tag="OO468_003446"
     CDS             complement(6022..6351)
                     /locus_tag="OO468_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR6260431.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6386..7846)
                     /gene="cls"
                     /locus_tag="OO468_003447"
     CDS             complement(6386..7846)
                     /gene="cls"
                     /locus_tag="OO468_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR6260432.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7989..8162
                     /locus_tag="OO468_003448"
     CDS             7989..8162
                     /locus_tag="OO468_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR6260433.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8217..9470)
                     /gene="kch"
                     /locus_tag="OO468_003449"
     CDS             complement(8217..9470)
                     /gene="kch"
                     /locus_tag="OO468_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR6260434.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2500 a         2253 c         2390 g         2443 t
ORIGIN      
        1 tttgttaagg catggatgca agctatagat tctgatacgg tcaataaaag agaatcgctt
       61 aacaattttg caaaatgtat tggcgagtaa gaaccgcatt tggtactttc cgggcaaccg
      121 ccagacgatt ctttattggt aatgagaata attaacaatt aaagagcgtc gcgaaagaat
      181 aatgtgtctc gacaggggag acacagtacg aatcgacata aggtgatcgt ctgaatcacc
      241 agaataaata aagtcggtga tagtaatacg taacgataaa gtaacctgac agcagaaagt
      301 ctccgagctt gtgcagggtc ccaatccggg attacacatg ctggttaata ccagtaatta
      361 taatgaggga gtccaaaaaa caatgaccaa catcaccaag agaagtttag tagcagctgg
      421 cgttctggct gcgctaatgg cagggaatgt cgcgctggca gctgatgtac ccgcaggcgt
      481 cacactggcg gaaaaacaaa cactggtacg taacaatggt tcagaagttc agtcattaga
      541 tccgcacaaa attgaaggtg ttccggagtc taatatcagc cgagacctgt ttgaaggctt
      601 actggtcagc gatcttgacg gtcatccagc acctggcgtc gctgaatcct gggataataa
      661 agacgcgaaa gtctggacct tccatttgcg taaagatgcg aaatggtctg atggcacgcc
      721 agtcacagca caagactttg tgtatagctg gcaacgttct gttgatccga acactgcttc
      781 tccgtatgcc agttatctgc aatatgggca tatcgccggt attgatgaaa ttcttgaagg
      841 gaaaaaaccg attaccgatc tcggcgtgaa agctattgat gatcacacat tagaagtcac
      901 cttaagtgaa cccgttccgt acttctataa attacttgtt cacccatcaa cttcaccggt
      961 gccaaaagcc gctatcgaga aattcggcga aaaatggacc cagcctggta atatcgtcac
     1021 caacggtgcc tataccttaa aagattgggt cgtaaacgaa cgaatcgttc ttgaacgcag
     1081 cccgacctac tggaacaacg cgaaaaccgt tattaaccag gtaacctatt tgcctattgc
     1141 ttctgaagtt accgatgtca accgctaccg tagtggtgaa atcgacatga cgtataacaa
     1201 catgccgatc gaattgttcc agaagctgaa aaaagagatc ccggacgaag ttcacgttga
     1261 tccatacctg tgcacttact attacgaaat taacaaccag aaaccgccat tcaacgatgt
     1321 gcgtgtgcgt accgcactga aactaggtat ggaccgcgat atcattgtta ataaagtgaa
     1381 agcgcagggc gacatgcccg cctatggtta tactccaccg tatactgatg gcgcaaaatt
     1441 gactcagcca gaatggtttg gctggagcca ggaaaaacgt aacgaagaag cgaaaaaact
     1501 gctggctgaa gcgggttata ccgcagacaa accgttgacc atcaacctgc ggtataacac
     1561 ctccgatctg cataaaaagc tggcgattgc tgcctcttca ttgtggaaga aaaacattgg
     1621 tgtaaacgtc aaactggtta accaggagtg gaaaacgttc ctcgacaccc gtcaccaggg
     1681 cacttttgat gtggcccgtg caggctggtg tgcagactac aacgaaccaa cttccttcct
     1741 gaacaccatg ctgtcgaaca gctcgatgaa taccgcgcat tataagagcc cggcctttga
     1801 cagcattatg gcggaaacgc tgaaagtgac tgacgaggcg cagcgcacag ctctgtacac
     1861 taaagcagaa caacagctgg ataaggattc ggccattgtt cctgtttatt actacgtgaa
     1921 tgcgcgtctg gtgaaaccgt gggttggtgg ctataccggc aaagatccgc tggataatac
     1981 ctatacccgg aatatgtaca ttgtgaagca ctaatggcaa tacgtggggc aggagtgtcc
     2041 tgctccacgg tgtctgattt ttatcgcatt acagaaggca caggccagaa ggtagggcaa
     2101 tgttaaaatt tattctacgt cgttgtctgg aagcgattcc gacgctattt attcttatta
     2161 ctatttcgtt ctttatgatg cgcctcgcac cgggaagccc ttttaccggc gaacgtactt
     2221 taccgccaga agtgatggcc aatatcgaag cgaaatatca tcttaatgat ccgatcatga
     2281 cacagtattt cagctacctg aaacaactgg cgcatggcga tttcggtcca tcgtttaaat
     2341 ataaagatta ttcggtcaat gacctggtgg catccagttt tcccgtttct gccaaactgg
     2401 gagccgccgc atttttcctt gcggtaatac tgggtgttag tgctggcgtt attgccgcat
     2461 taaaacaaaa caccaaatgg gactataccg tgatggggct ggcaatgacc ggggttgtta
     2521 tccccagttt tgtggttgcg ccattattag tcatgatatt tgcgatcatt ttgcattggc
     2581 tgccgggcgg tggctggaat ggtggggcgc ttaaattcat gatattgccg atggtggcgt
     2641 tgtcactcgc ctatatcgcc agtattgcgc gtattacccg tggctctatg attgaagtat
     2701 tacactccaa ctttattcgt actgcccggg cgaaagggtt acctatgcgg cggatcattt
     2761 tacgccatgc attaaaacct gctctgttac ccgtgctctc ctatatgggc cccgcatttg
     2821 tcggcattat taccggttct atggtcatcg aaaccattta tggtttgccg gggattgggc
     2881 aactgttcgt taatggcgca ttgaaccgtg actattcctt agtgttaagc ctgaccatcc
     2941 tggttggtgc gttaaccatt ttgtttaatg ccattgtcga tgtgctatat gcggttatcg
     3001 acccgaaaat ccgttactga tactggagct cgcgatgatg ttaagtaaga aaaacagcga
     3061 gacgctggaa aatttcagtg aaaagctgga ggtcgaaggg cgcagcttgt ggcaggacgc
     3121 acgtcgacgt tttatgcata accgtgcggc ggttgccagt ctgatagtgc tggtgctgat
     3181 cgcgttattt gtaatcctgg caccgatgct ttcccagttt gcctatgacg atactgactg
     3241 ggcgatgatg tccagcgccc cggatatgga gtccggtcac tactttggta ctgactcatc
     3301 cggtcgcgac ctgcttgtgc gcgttgcgat tggcgggcgt atctcactca tggtcggtgt
     3361 tgctgcggca ctggtggcag tggtcgtggg gacactttat ggttcgcttt ccggttatct
     3421 gggcggtaaa gtggattcgg taatgatgcg tctgctggaa atcctcaact ccttcccatt
     3481 catgttcttc gtcattttgc tggtgacctt tttcggccaa aacatcctgc tgattttcgt
     3541 ggcgattggc atggtttcct ggctggatat ggctcgtatt gtgcgtgggc aaaccctgag
     3601 tctgaagcgc aaagagttta ttgaggcggc acaagttggc ggtgtatcga cgccgggcat
     3661 tgttattcgc cacattgtgc cgaacgtact cggtgtggtg gtggtctacg catcgctact
     3721 ggtgcccagc atgatcctct ttgaatcttt ccttagcttc ctggggttgg gtacgcaaga
     3781 gccgttaagc agctgggggg cattgctgag tgatggcgcg aactcgatgg aagtctctcc
     3841 atggttactg ttgttcccag cgggattcct tgtggtgaca ctgttttgtt tcaactttat
     3901 cggcgatggc ttgcgtgatg ccctcgaccc gaaagatcgt taaggagtgc agccatgagc
     3961 gtaattgaaa ctgcaactgt gccgctcgca caacaacagg ctgacgcact gctgaacgtg
     4021 aaagatttgc gtgtcacctt tagtaccccg gacggcgacg tcacggcggt caatgattta
     4081 aatttttccc tacgtgccgg agaaacgctg ggcattgtag gtgagtctgg ttcgggtaaa
     4141 tcgcaaactg catttgcgtt gatgggcctg ttggctgcca acggacgcat tggcggatcg
     4201 gcaaccttca atgggcgtga aatcctcaat ttgccagagc gtgaactcaa taaactgcgc
     4261 gctgaacaaa tctcaatgat ttttcaggac ccaatgactt cgttgaatcc ctatatgcgc
     4321 gtcggtgagc agttgatgga agtgctgatg ctgcataaga acatgagcaa agctgaggcg
     4381 tttgaagagt cggtgcggat gctcgatgcg gtaaaaatgc cggaagcacg taaacgcatg
     4441 aaaatgtacc cgcacgaatt ttctggcggc atgcgtcagc gagtcatgat tgcgatggca
     4501 ttgctatgtc gacctaagtt gctgattgcg gatgaaccca ctacagcgct ggacgtcacc
     4561 gtacaggcgc agatcatgac gctattgaat gaactgaagc gggaatttaa tactgccatc
     4621 attatgatca cccacgatct tggcgtggtg gcggggatct gtgacaaagt gctggtaatg
     4681 tacgccgggc gcacgatgga atatggcaat gcgcgcgatg tcttttatca acccgttcat
     4741 ccttattcta tcggtttgct caacgcggtg ccgcgtcttg atgcggaagg tgaaacaatg
     4801 ttgaccatcc cgggtaatcc gccaaacttg ctgcgattac cgaaaggttg cccgttccag
     4861 ccacgttgtc cacatgcgat ggaaatttgt agtagcgcac cgccgctgga agagtttacg
     4921 cctggccgtc tgcgtgcttg ctttaaaccg gtggaggaac tgttatgaat gctgtaactg
     4981 aaggaagaaa agtcctcctt gaaatcgccg atcttaaagt gcactttgaa atcaaagatg
     5041 gcaaacagtg gttctggcaa ccgccgaaaa cgctcaaagc cgtcgatggt gtcactcttc
     5101 gcctgtatga aggggaaaca ttaggtgtgg taggggaatc gggatgcggt aagtccacct
     5161 ttgctcgcgc catcatcggt ttggtcaagg cgaccgacgg tcatgttgcc tggttaggta
     5221 aagagttgct gggcatgaag cccgatgaat ggcgtgccgt tcgcagtgat attcagatga
     5281 ttttccagga tccgttggca tcgctaaacc cgcgtatgac catcggcgag atcatcgctg
     5341 aaccactgcg tacttatcat ccgaaaatgt cacgccagga agttcgcgag cgcgtgaagg
     5401 cgatgatgct gaaagtcggg ttattgccta acctgattaa ccgctatccg catgagttct
     5461 ccggtgggca gtgccagcgt atcgggattg ctcgtgctct tattcttgaa ccgaagctga
     5521 ttatctgcga tgagccggtt tcggcgctgg acgtgtcaat tcaggcgcag gtggttaacc
     5581 tgctccagca gctgcaacgt gagatgggat tgtcattaat ttttattgct catgacctgg
     5641 ccgtggtaaa acacatttcc gatcgtgtgt tggtgatgta tctcggccat gcagtagaac
     5701 tggggaccta tgatgaggtc taccacaatc cactacatcc ttacaccagg gcattgatgt
     5761 cggcagtccc catacctgat ccggatctgg agaagaacaa aaccatccag ttactggaag
     5821 gggaattacc gtcgccgatc aacccgcctt ccggttgtgt tttccgtacc cgttgcccga
     5881 ttgccggtcc ggagtgcgcc aaaacacgtc ctgttctgga ggggagtttc agacacgccg
     5941 tttcttgcct gaaagtcgat ccgctttaaa aacaataagg gctgacagtt gtcagccctt
     6001 tttcacgcta aaagcgatta tttattcccg ccagatgata tggcaaagtt tgtgatcttt
     6061 ttcgcggcat aacagaatgc gggcgaaaac atcgctgatt tcaccatctt cactgtccgc
     6121 cagaccaatc accacttcgg caaaaaagtc cgggttcaaa tcgaaatcaa catgttcctg
     6181 ccagtcttcc gccggatcga ataactccgc gccgccgcgc tcttcaaact gaagattgaa
     6241 cagcagaaca tcagctggat cgagattgtc ggcagccagt tcgagaaaaa tatcgtaggc
     6301 ctgctcaagc gtttcatctt cagtcaggcg attgtttaga tccatatcca tagtcactac
     6361 ctgtttaacc tctgttggcg acgttttaca gcaacgggct gaagaagtaa aacagtcgct
     6421 cggcgacacg ttgccataat ggacgtttta gccataaacg ggcatcgagc agacgtgaac
     6481 gcgaaatata atcgtcctga acggcggcaa ggtctgcacc aaaaccttta tcgtcgattg
     6541 ccaaggtaat ctcgaaattc agccacagac tacgcatatc aaggttaacg gtgccaacca
     6601 gacttagttc gccatcgacc agcacgctct tggtatgcag taacccgcct tcaaactgat
     6661 aaattttaac cccagcagca agcagttccg taaagaatgc gcgactggcc cagccgacca
     6721 gcatcgagtc attttttcgc ggaaggataa tactgacatc caccccgcgc tgcgccgccg
     6781 tgcaaatcgc atgaagtaaa tcatcgcttg gcacaaagta gggcgtggtc atgatcaaat
     6841 attcatgcgc cgaataagcc gcagtcaata atgcctggtg aatgagatct tccggaaagc
     6901 cggggccaga agcaattgtg tgaatggtgt gaccgctggc ctgttcaaac ggcataatat
     6961 tgacatctgg tggtggcggc agaatacgtt ttccggtttc aatctcccag tcgcaggaat
     7021 aaataatccc catcgcggtg gcgatggggc cttccatacg cgccatcaga tcaatccatt
     7081 gccctacgcc cgcatcttgt ttgaagtagc gaggatcgac catattcatg ctgccggtgt
     7141 acgcgatgta attatcgatc atgatcatct tgcgatgttg gcgcaggtcc atacggcgta
     7201 aaaacacacg catcagattg acctttaagg cttcgaccac ttcaataccg gcattacgca
     7261 ttagctcggg ccacgggctg cggaaaaaag ccacactccc ggcggagtcg agcatcaatc
     7321 ggcaatgaat accacgccgt gcagccgcca ttaatgattc agccacctgg tccgccatgc
     7381 cgccgggctg ccagatataa aacaccatct caatattatg gcgcgcgagc tggatgtcgc
     7441 ggattaacgc ctgcatcaca tcatctgact cggtcatcag ctgtagctga ttccctttga
     7501 ccccagcgat cccctgacga cgctcgcaaa gcttgaataa tggcgcagcg acactgctat
     7561 tttcttcggc gaagatatgc ttacaggctt taaggtcgtt aagccatttt gcggtggaag
     7621 gccacatcgc tctggcgcgc tcagcgcggc gtttgcctaa atggagctcg ccaacggcaa
     7681 gataggcaat aattccgact aacggcagaa tgtaaataat caacagccag gccatcgcgg
     7741 agggaactgc gcgtcgtttc attagaatgc gtaaagttac gcctgcaatg agcaaccagt
     7801 atcccagaat ggccaaccaa ctcaccaacg tataaacggt tgtcatagat tagaaatcct
     7861 tttgaaagcg cattgttatg agtttacgca tatggattca tctggcaaat aaaaacgcgg
     7921 gaaaagcact ggtcagcgtc gggggcgggg gtataatgac cattctgtta ttgcatagag
     7981 tagttaacat gaagcggagt agaacggaag tggggcgctg gcgcatgcag cgtcaggcta
     8041 gccgacgtaa atcgcgttgg cttgaggggc aatcgcgccg aaatatgcgt atccacagca
     8101 tcaggaagtg cattctaaac aaacagcgta actcgttatt gtttgcgatc tacaatatct
     8161 aaatgtactg ggcaccattg cggtgcccaa taagtttact cttccgtctt taaactctat
     8221 ttttgcgccg attctttact gtcagctttc gtttcttgtt catcgttatc gctgaaaata
     8281 ccatgacctg agttcaacaa cattgaaacg agcatatcat tattaatctc ttcaccattt
     8341 aaaactcgcg ccagaatttc gctgccaaac agttgcggtg agagaatgat atccggatgt
     8401 accatcttaa tcttattcag gtttttgcta tcactgacgg cgagaactgt tttgacatca
     8461 ctgctcatat cttttgccga gagtacaaca aacgcgttat ctgcatcgtt atcactcagc
     8521 gccagaatgg cccggcaacg atcgattccc gctttcttta atactgaact gtcattactg
     8581 tcaccgggga taacatcagc gttatcgcct aagcgttgct caagttgctt gatatcatct
     8641 tcaggcaagt tgctgataac cgttacgttt tgtccgcgtt gattcagttg caggatcgta
     8701 ttgatggcga gaatcgaatg tccgcaaaca ataaaatgat ctttacgatg cattgtatga
     8761 ttgtttcctt ttaaaagttt gttgaatccc ccgcggataa gcggaccaaa aattgaggtc
     8821 atagatgtgg caaaaacggt aatgccggaa ataatgaccg aaatagtgaa caatcgtgct
     8881 gattcagaaa cagggacaat atctccatag ccgacggttg acatggtttc tatcgaaaaa
     8941 tagaacgcgg tcatcaaact ttctattcgc ggattaaaac cttcgcttaa ataaagcgca
     9001 ccgtaggttg agtaaaacag taatgtcgtg aaactaataa atgcaaaaat tgtcccggct
     9061 gcggcgctac tgtgggagaa gtctttgcgc agtatcagca aaaacaccag cgtaaaaatg
     9121 caaaatccaa tactaaattt cagccaggga taaaaatgca gggtgtaaat tagcgctatc
     9181 aacaacaaaa tgatactgat tgcccatgcg agctttgccc ggaacagtaa gccaatggag
     9241 ttcaggacaa gaaagacgcc gagcataaat agtggggcgt ttgccagcaa ggagagatcc
     9301 aactctgaaa atgctttgat atggaaaata tcaagcaaat tgaccgacat accatagatt
     9361 gttttaaaaa taagcaatcc atttaaaaag acggccagcg cgagaatgtc gtgccgtagg
     9421 gtcacccata aatttgttgc tgtttgtttg aatgtagccc agtgactcac tgatacctct
     9481 gtaaccacaa ttttgtttaa ttatcttagc gtgcacattt tctgaacata catgcagcgc
     9541 gtgaatgtgt taaacaatag tagatgactt tatccgcagt aatcaa
//
LOCUS       DAJTUP010000128         9354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTUP010000128 DAJTUP010000000
VERSION     DAJTUP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.128"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..2469)
                     /gene="torA"
                     /locus_tag="OO468_003450"
                     /pseudo
     CDS             complement(1..2469)
                     /gene="torA"
                     /locus_tag="OO468_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(2469..3641)
                     /gene="torC"
                     /locus_tag="OO468_003451"
     CDS             complement(2469..3641)
                     /gene="torC"
                     /locus_tag="OO468_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR6260435.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            3728..4420
                     /gene="torR"
                     /locus_tag="OO468_003452"
     CDS             3728..4420
                     /gene="torR"
                     /locus_tag="OO468_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR6260436.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(4393..5421)
                     /gene="torT"
                     /locus_tag="OO468_003453"
     CDS             complement(4393..5421)
                     /gene="torT"
                     /locus_tag="OO468_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR6260437.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            5504..8215
                     /gene="torS"
                     /locus_tag="OO468_003454"
                     /pseudo
     CDS             5504..8215
                     /gene="torS"
                     /locus_tag="OO468_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            8317..>9354
                     /locus_tag="OO468_003455"
     CDS             8317..>9354
                     /locus_tag="OO468_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR6260438.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYR"
BASE COUNT         2173 a         2522 c         2435 g         2224 t
ORIGIN      
        1 cacctgctcc actgttccat tgtacttctc aatttccacc agcgtagtgt gcgcactggt
       61 cgcctgcgcc agctgcgaag tcccgatgtc gatggtcagc acgttggggt taccgtattt
      121 gcacagcgca ccaggctcgc cgcctttatc tggatcgtac catgcccctt cgtgaattcg
      181 cgccacgccg ggtgcatagc ggtcagaaac tactgctcct gccaacacct gaccgcgagc
      241 gttaaagacg cgtaccacat caccgttacg aataccgcgc gcgctggcat cctgcgggtt
      301 aatgaacact ggctctttac ccgctaccgt atattgctga cgcagcgttt ccgactcaca
      361 tagctgcgag tgaagtcgga aatccggatg cacagattgc agatgcaacg gatacttctg
      421 cgagccgggc ccaccgtggg agcgttcgat tttctcaaac cacatcggat gcccctgaca
      481 atcgtcgtag ttcatatcgg cgatggtttt cgagtagatc tcaatcaggc cactcggcgt
      541 gcccagcggt tcgagatccg gatcttcgcg gaatgcctgg tggcgaacaa acatctgcgg
      601 atggtcaaac tcgacgtact ctttgttatt ccagaagtca tcaaacgctg gcagatgaac
      661 gccgcgtcct ttgccttgtt gtacaccttc ctgccagatg cgtttcagcc agcccatttc
      721 gtccagccct tcggtaaagg cttcttcacg attaaagcga cggcacagct cgcggaaaat
      781 atcaaagtcg ttgcgcgcct cgaactacgg cggcaccacc tgtttcatgg cgataatgcc
      841 acggttggag tggttgccgt actggtcgag atcgttacgc tcaaactgcg tggtcgcagg
      901 cagcacgata tcggcaaagc ggcaggttga ggtccactgg ttatctatgg cgataaccgt
      961 ttccagcttg cgccagcctt caataatgcg gttgatctgc tgatggcgat ggaatgggtt
     1021 agttccggca aaaatacaca ttttcagcgg cggcagtttt accgatttac cgttccagtt
     1081 gatcactttc cccggttcga ggatcgcatc gataaaacgg gcaatcggaa tagtgctgct
     1141 gtagcctttg tagtcactgt tgtcgtgaac aggcggaatc gacgtagagc cggagaaacc
     1201 actcagaata acgcctttac gccccggcgt gcctgcgccg ttgtagtgcc agccaaaacc
     1261 accacctggc aggccaattt gccccagcat cgccgccaga accacaatca tccacgccca
     1321 ctgttcaccg tgctgcatac gctgcacgca ccagccagca ataatttgcg ttctgttcgc
     1381 cgccatctgc cgcgccagcc cacgaatggt ttcggcatca atgccggtca gtttttcagc
     1441 ccatgcggca tctttcggct gaccgtcttt ctcacccagc agatacggca ggaactgctc
     1501 aaaacccaca cagtagttag cgaggaagtt tttgtcgtac aggttttcac tgtacagcgt
     1561 atgtgccagc gccagttgca gcggcacgtc agtttgcggg ttaaccgcaa tgtgcttcac
     1621 atgctcgcgc cccagatact catgggtgga tgtgacaacc ggatcgatgc tgatgacctc
     1681 aacttcaccg gcggcgactt tcgcttttag ctgctcgtaa tattcataaa catcgtgatc
     1741 cgggcaccac cagttcgctt gctggttttt cagcaaatcg gagccccaca gcacaatggt
     1801 tttgctgttc tgcaatacca gcggccagga ggtttgctgt tcatacactt ccattgaacc
     1861 gactacgcgc ggcaggatca cctgcgcagc accggtagag taatctccgc ccgtaccaac
     1921 gctattacca tgcaaggcaa tagctttcgc cagcatcccc gaagcgttat ggaacatccc
     1981 cgtcgattgc caaccactgg cggtcagcaa ggcactcggc ccgtgagttt tctgtacgcg
     2041 ttccagttct tcatagaaca tgtcgagggc ttcatcccag ctcacgcgca caaaacggtt
     2101 atcaccgcgc tgggaggtgt cgctcagatg gcgcttacgc agccagtcca cgcgtaccat
     2161 cggataacga atacgtgccg cgttgtgtac gtgatccggc aatccggcga tcattttcga
     2221 cggatattta tccagttcga acggttttgc cgccacaaag cgaccatcct tcaccatcgc
     2281 gcggatagcc ccccagtgcg acccggtaag aatgccctct ttcgagatga cagcctcagt
     2341 cgccgcttgc gccgcagttg cacggcgcgg cgttaacaat gacggcccca gcatcccggc
     2401 gacggttaag ccgccgagtt gtgccagaaa acgccgacgt gatgcctgaa agagatcgtt
     2461 attgttcatt atttttcttc cttcttatcg ccgtgagcct tacctgcggt gtcagacgca
     2521 ttcatttgca gatatttcaa caaagtgcgt tcttcacgtt tatcgagact ggtaaagcca
     2581 atcatgccgt tgagcgtgcc gatccaaccg ttagcgtcaa agtgggcgat ttccggtgcg
     2641 ccgtggcact cgttacaggt gccgttgtac aacgaatccg cataggccca gatcggtttg
     2701 atatcgttca ccatgtcgcc tttcttcatc cacgcggtgg cctgcaactt gctccactcg
     2761 gtattggtgt cggcaacggt ggttttctcc agcgttttca cctgctgctg cacatcacca
     2821 cgaatcgagg caacaaagat gcgtttacct gggaattggg tgagtacacg ctgacgtccg
     2881 gcgctttccg tccagccggt aatttcaatt tgcagccagt cgccgtcacg tttaaggact
     2941 ttcacttccg aagcaggcag cagagaacca gaggcttctt tatcgccttt cgccgcataa
     3001 attggcttaa tatcaataga gtacagcgtg tcaccactgt cattagcact ggcgcgcagc
     3061 tcatcgaact gcttacggaa gccgctactc atatccggta actggtgggc aataccttta
     3121 tgacagtcga tgcaggattg attatctttc gctgccacct tcatctgacg tgccgcttca
     3181 ggatgctgcc tggcatgatc catcgcatcg tagttatggc aggagcgaca ggttgccgag
     3241 ttgttttctt tcattcgcgc ccattcacgc tcggcaagtt ccgcgcgttt ggcttcgaat
     3301 ttttcaggtg tatcaatgga gtgggcaata aaggtctggt aaatgtcgtt gctcgcttcc
     3361 agtttgcgct tcaccatgcc tggaatatcc ggcgggatat gacagtcatg gcattcagct
     3421 cgcacgccgg aggcgttctg gaaatgcacc gactgtttat attcttcata caccggttgc
     3481 atactgtggc aactgacaca aaattcggtt gtgctggtga ctttgatccc aacgtgtggc
     3541 aatacaatca gcgcaatgcc aatcacaatc ccaattgcga ccagcgccag taccgaccaa
     3601 cgagcactgg gtcggcgtag cgcgttccag agtttccgca taataacccc tgtagaatta
     3661 tggtttagtg aagcgatctt aatgagcaaa tatgaacaga tatgaacaga atgagtaaaa
     3721 ccctctgatg ccacatcaca ttgttattgt tgaagataag ccggttaccc aggcgcgatt
     3781 acaatcctac ttcactcagg aggggtatac cgtttccgtt acggcgagcg gtgccgggct
     3841 acgggaaatt atgcagaatc agccggtaga tttaattctg ctggatatca acttacccga
     3901 tgaaaatggc ctgatgttaa cccgcgccct gcgagaacgc tcaacggtgg ggattattct
     3961 ggttaccgga cgcagcgatc ggattgaccg tattgttggg ctggaaatgg gcgcagacga
     4021 ttacgtcacc aaaccgctgg aactgcgcga actggtagta cgggtgaaaa atctgctctg
     4081 gcgaatcgac ctcgcgcgac aagctcaacc gcacactcag gactactgct atcgctttgc
     4141 gggttattgc ctgaatgtgt cgcgccatac gctggagcgg gatggcgagc cgattaaact
     4201 gacccgcgca gagtatgaaa tgttggtggc atttgtgact aatccgggcg aaattctcag
     4261 ccgtgagcgt ctgctgcgta tgctttctgc gcgtcgggtg gaaaaccctg acctgcgcac
     4321 cgtcgatgtg ttaattcgtc gtttacgtca taaactcagc gcggatttac tggtgacgca
     4381 acatggtgaa ggttatttct tagccgctga tgtgtgctga taaaaataga ccggacgaaa
     4441 tccccccggt gacagcgagc ggcggatatg ttctcggtcg gcatttttcg gcgtcagaac
     4501 taaaatcggt gggctgacat tatcagacac cgattgcccc tgtaattgcc tgatggcctg
     4561 ctcaactgcc agttccccct gccagaccat ttgatcgctg gcagccataa tcactcttcc
     4621 ccgcttcagc ccgcgataca cctgatgtga aagataaaac gacaccacgg taagcggcgt
     4681 tttcaggtta cgcccttcac ccattgccgc ctctgccgca atggccgttc cggcaacgac
     4741 gtcgatttct gggtggcgtt ccagcatctc ctgcaacagg ttgcgctgga tttcaatatc
     4801 gttatcacca agcgcaatat caacaatacg caccgggctt ccggcaatgg ctgcgcgaaa
     4861 accctcgacc atctccttac tgcccccggc gttatcgggt ccgggcatca gcagcacgtt
     4921 cagtggttta ccgtgcgccc attgcaccaa atatcgccca ggttgatagc ccatctgaaa
     4981 ccagggtaca ccaacgcggc ttttcacctg gggatcatca agagcattta ccagttcgat
     5041 caccggcaga tttgctacct gcttttgcag gtcgggaaat gacgtcgtgc tactaccgag
     5101 caaaatggcc tctgcgcccc actgtttaca ctggtcgatt tgtgcttgct gggtagccaa
     5161 ctggctgtag ccgcctgcct ccagcacttt taaatccaca ccgtagcggc gagcagcctc
     5221 ctgcatacca tagttcaacg ataaccagta tgaatctttc aggctgggat aaagcgcgca
     5281 cagtttccat acgcgtttgg ctttaagcgg catagaggct tgcaccgtga aatgctgcgc
     5341 atcatgccag cgcaacaggt tatcagccga aaatgccgac aacatgaaaa gggaaagaag
     5401 taaaaatagc agtacgcgca tgatagcctc atcaataata aggctttatg ctagatgcat
     5461 tccactttgc gactcaacct ttttcacctt aagtgcaccg accgtgaatt taaccctgac
     5521 ccgaagactc tggatgggct ttgccctgat ggcgctgtta accctgacca gtaccctggt
     5581 gggatggtac aacctgcgct ttatcagcca ggtggaaaaa gacaatactc aggcattgat
     5641 tcctaccatg aatatggcgc gccagttgag cgaagccagc gcctgggaac ttttcgccgc
     5701 gcagaacctg accagtgccg ataacgaaaa gatgtggcag gcgcaggggc gaatgctcac
     5761 cgcataaagc ctgaagatta atgcgttgct gcaagcgtta cgggaacaag gttttgatac
     5821 caccgctatt gaacaacagg agcaggagat ctcccgttca ttacgtcagc aaggggaact
     5881 ggtggggcgg cgtctgcaac tacgccagca acaacggcaa ctcagtcagc agatagctgc
     5941 cgccgatgag atcgcacgcc tggagcaagg tcaggcgaat aatgcgacaa cttccgctgg
     6001 agcgacccag gccgggattt acgatttgat cgaacaagat cagcgtcagg ctgctgaaag
     6061 tgcactcgat cggctgattg atatcgatct tgagtatgtt aaccagatga atgaactgcg
     6121 ccttagcgct ctgcgggtgc agcaaatggt gatgaatctg gggctggagc agatccagaa
     6181 aaatgcacca acgctggaaa agcagctcaa taatgcggtg aaaattctgc aacgtcggca
     6241 aatacgcatt gaagatccgg gagttcgtgc gcaggtcgca acaacgttaa ctaccgttag
     6301 ccaatatagc gatctgttgg cgctgttcca gcaggacagt gaaatcagca atcacctgca
     6361 aactctcgcc caaaataata tcgcccagtt cgcgcagttt agtagcgaag tcagtcagct
     6421 ggtcgacacc attgagctgc acaatcagca tggactggca catctggaaa aagccagtgc
     6481 acgcgggcaa tacagcctgt tattgctggg gatggtttca ctttgcgcac tgattatgat
     6541 cctctggcgc gtggtttatc gctcattcac gcgtccactt gccgaacaaa cgcaggcgct
     6601 gcaacggctg ctggacggtg atattgactc ccctttcccg gaaaccgctg gcgtacggga
     6661 gctggatacc atcgggcggc tgatggatgc gtttcgcagc agtgttcatg cactgaatcg
     6721 ccaccgcgaa cagctggcgg cgcaggtcaa agcgcgtaca gctgaattgc aggaactggt
     6781 gatagaacac cgacaggcac gggcggaagc agaaaaagcc agccaggcaa aatcggcgtt
     6841 tctgacggcg atgagccatg agatccgcac accgctgtac ggtattctcg gcactgctca
     6901 actgctggca gataaccccg cacttaacgc ccagcgtgat gatttgcagg cgattaccga
     6961 ctctggcgaa tcgttgctga ccatcctcaa cgatattctc gattattcgg ctatcgaagc
     7021 aggtggcaaa aatgtttcag tcagcgatga gccctttgaa ccgcgcccgc tgctggaaag
     7081 taccctgcaa ttaatgagcg gacgggtgaa aggtcgcccg attcgcctgg caacggcaat
     7141 tgccgatgat gtaccgagcg cgttaatggg cgatccacga cgtattcgtc aggtcattac
     7201 caacctattg agcaatgccc tgcgttttac tgacgaaggg cacattatcc tgcgtagtcg
     7261 cactgatggc gagcaatggc tggtcgaagt ggaagacagc ggctgcggta ttgatcccgc
     7321 gaaactggca gaaatcttcc agccatttgt gcaggtaagc ggcaaacgcg gcggcaccgg
     7381 gctggggctg actatcagta gccgtctggc ccaggcgatg ggcggcgaac tgagcgccac
     7441 cagcacgccg gaggttggaa gctgtttttg tttacgcttg ccgttacgtg ttgccactgc
     7501 acccgtgcca aaaacagcca atcaggcggt gcgtcttgac ggtttacgtt tgctgttaat
     7561 tgaagataac ccgctaaccc agcgaattac cgttgagatg ctgaacacca gtggtgcgca
     7621 ggttgttgct attggcaatg ccgcgcaggc tttagagaca ctgcaaaata gcgaaccgtt
     7681 tgctgccgca ctggtggatt ttgatctgcc ggatatcgac ggcattaccc ttgcccggca
     7741 actggcacag caatatccgt cgctggtttt gattggcttt agcgcccatg tcattgacga
     7801 aacgctgcgc cagcgtacca gttcactatt tcgcgggatt atccctaaac cggtgccgcg
     7861 tgaagtgctc ggtcagttac tggcgcacta tctccaactg caagccaata acgatctacc
     7921 actggatgtc tcgcaactca atgaagacgc tcagttaatg gggacggaga agatccacga
     7981 atggctggca ttatttaaac aacatgccct gccgcttctc gatgaaatcg acattgcccg
     8041 cgccagccag gacagcgaga aaataaagcg tgccgcgcat cagctaaaaa gcagttgctc
     8101 gagtctggga atgcgtagcg ccagtcagct atgcgcacaa ctggagcagc agccattatc
     8161 tgcccccctt ccacacgaag aaattacacg cagtgttgcc gctctggaag catgattgaa
     8221 taagaaagac ctgaacgcga tttgaatcaa tatcagccag ttaagaataa ctagaatagt
     8281 ggcatttggt atgcaactta acggactggc aaaatcatgg cagagaaaaa aagaacccgc
     8341 tggcagcggc ggccaggcac gacgggcggc aaattaccgt ggaatgactg gcgcaatgcc
     8401 acgacctggc gtaaagcgac gcaattctta ctattggcaa tgaatattta tattgccatc
     8461 acgttctggt attgggtgcg ctattacgaa acggcaggta gtacgacatt tgtcgccaga
     8521 ccgggaggta tcgaaggctg gctaccgatt gccggtctga tgaatctgaa atatagcctt
     8581 gcaacaggtc agttaccgtc cgtccacgcc gccgcgatgc tgttattggt cgcttttatc
     8641 gtcatcagtc tattactcaa aaaggccttt tgctcatggt tatgcccggt tggtacgctt
     8701 tctgaattaa tcggcgatct cggtaacaaa ctgtttggtc ggcaatgtgt ccttccccgc
     8761 tggctggata ttcctctgcg cggcgtgaag tatttgctgt tgagtttatt tctctatatc
     8821 gcgttattga tgcccgctca ggcgattcac tattttatgt tgtcgcccta tagcgtggtg
     8881 atggacgtta aaatgctcga tttctttcgt catatgggga ccgcgacatt aatcagcgtg
     8941 accgttttgc tgattgccag cctgtttatt cgccatgcct ggtgtcgtta tctttgccca
     9001 tatggcgcgc tgatgggcgt ggtttcgcta ttgtcgccgt ttaagattcg tcgcaatgcc
     9061 gaaagttgta tcgattgtgg caaatgcgca aaaaagtgcc catcacggat cccggtagat
     9121 aaattaattc aggtacgaac agtggaatgt accggctgta tgacttgcgt agagtcatgt
     9181 ccggtagcct caacattgac cttttcactg caaaaacctg cagcaaataa aaaagccttt
     9241 gcgttgtctg gctggttaat gacgctactg gttctgggga ttatgtttgc ggtgattggt
     9301 tacgcaatgt atgcgggagt atggcaaagc ccggtgccgg aggaattgta ccga
//
LOCUS       DAJTUP010000129         9291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTUP010000129 DAJTUP010000000
VERSION     DAJTUP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.129"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO468_003456"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO468_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR6260439.1"
                     /translation="ERMQTDTLEYQCDEKPLTVNLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO468_003457"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO468_003457"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR6260440.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="OO468_003458"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="OO468_003458"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR6260441.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="OO468_003459"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="OO468_003459"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR6260442.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO468_003460"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO468_003460"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR6260443.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO468_003461"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO468_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR6260444.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO468_003462"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO468_003462"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR6260445.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO468_003463"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO468_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR6260446.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO468_003464"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO468_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR6260447.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO468_003465"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO468_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR6260448.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2383 a         2326 c         2357 g         2225 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 tctgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAJTUP010000130         9233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTUP010000130 DAJTUP010000000
VERSION     DAJTUP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.130"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO468_003466"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO468_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR6260449.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO468_003467"
     CDS             1828..3147
                     /locus_tag="OO468_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR6260450.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO468_003468"
     CDS             3445..3486
                     /locus_tag="OO468_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260451.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO468_003469"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO468_003469"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR6260452.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO468_003470"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO468_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO468_003471"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO468_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO468_003472"
     CDS             5815..6711
                     /locus_tag="OO468_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR6260453.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO468_003473"
     CDS             6708..7682
                     /locus_tag="OO468_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR6260454.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO468_003474"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO468_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO468_003475"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO468_003475"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR6260455.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2256 a         2255 c         2316 g         2406 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat ata
//
LOCUS       DAJTUP010000131         9204 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTUP010000131 DAJTUP010000000
VERSION     DAJTUP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.131"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..654)
                     /gene="bglB"
                     /locus_tag="OO468_003476"
     CDS             complement(<1..654)
                     /gene="bglB"
                     /locus_tag="OO468_003476"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR6260456.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     "
     gene            complement(673..2550)
                     /gene="bglF"
                     /locus_tag="OO468_003477"
     CDS             complement(673..2550)
                     /gene="bglF"
                     /locus_tag="OO468_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR6260457.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2684..3505)
                     /gene="bglG"
                     /locus_tag="OO468_003478"
     CDS             complement(2684..3505)
                     /gene="bglG"
                     /locus_tag="OO468_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR6260458.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(3792..4517)
                     /gene="phoU"
                     /locus_tag="OO468_003479"
     CDS             complement(3792..4517)
                     /gene="phoU"
                     /locus_tag="OO468_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR6260459.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4532..5305)
                     /gene="pstB"
                     /locus_tag="OO468_003480"
     CDS             complement(4532..5305)
                     /gene="pstB"
                     /locus_tag="OO468_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR6260460.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5397..6287)
                     /gene="pstA"
                     /locus_tag="OO468_003481"
     CDS             complement(5397..6287)
                     /gene="pstA"
                     /locus_tag="OO468_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR6260461.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6287..7246)
                     /gene="pstC"
                     /locus_tag="OO468_003482"
     CDS             complement(6287..7246)
                     /gene="pstC"
                     /locus_tag="OO468_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR6260462.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7333..8373)
                     /gene="pstS"
                     /locus_tag="OO468_003483"
     CDS             complement(7333..8373)
                     /gene="pstS"
                     /locus_tag="OO468_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR6260463.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8621..>9204)
                     /locus_tag="OO468_003484"
     CDS             complement(8621..>9204)
                     /locus_tag="OO468_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6260464.1"
                     /translation="YIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGD
                     ITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDD
                     ISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSG
                     SVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2353 a         2424 c         2147 g         2280 t
ORIGIN      
        1 ggggatcagg ctatgatagg ctttaactgc ccgcgcactg gcaaccagtt gatgatggat
       61 cgcctgataa acttccgcct cgccactctc ttctgccagc cccacgcccg tgaatggcgc
      121 gtgtaacgac atattgattt cattaaacgt cagccataac gctactttat gttggtagcg
      181 ggtaaagacc gtgcgggcgt aatgctcgaa gtgatcgatg accgcccgat tagcccaacc
      241 gccgtagttt ttcaccagcc catatagcat ttcgtaatgg gataacgtta ccagcggctt
      301 gatccccgcc tgcgccattt catcaaacag ccgatcgtaa aacgctaacc ccgcttcatt
      361 cggttcggct tcgtcgccct gagggaaaat tcgcgcccag gcaatggaaa tacgcagaca
      421 ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtaat aaaaatcgat
      481 ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc ccattacgcc
      541 atgaggctgt aaatctgagg tcgtgatccc tttgccatct tccagccagg caccttccac
      601 ctgattggca gctgttgcgc caccccaaag aaatgtttct ggaaatgctt tcataattaa
      661 ctccttttat cgttagcgaa tgatggataa cagcggttca cctgcgctta tctgcgccgt
      721 gccgtggggt aatacgtctg taaaatcatc gctattactg attaataccg gcgtcgtcag
      781 atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat cgcctgtatt
      841 gaccttgtca cccacgttga cgtgagcgga aaagaatttg ccgtccagtt ttacggtgtc
      901 gataccgaca tgaatcagga tctccacacc atcatctgac tcaatgccaa tggcgtgtaa
      961 tgtggcgaac aacgaagcaa ttcgacccgc aaccggagaa cgcacttcac caaccgaggg
     1021 cagaatggcg atacctttac ccaacaggcc actggcaaac gtggtatcag cgacgtgaat
     1081 gagcggcaca atctctcccg tcatcggtga acagataccg ccctgctcag gtggtgtaat
     1141 aacctctggt gttttctctt gcggggcacc ctgcgctggc tgacgtttac tggtgatgaa
     1201 atgaagcatc aacgtaccaa caaatgcgca acctatggca atgacaccgc caataacgct
     1261 ggcccagacg gtgaaatcaa tccccgttga cgggatggtc tgcatgaagg tgaaaatact
     1321 tggcaaacca aaggagtaaa ctttcgtttg cgcgtagcca atgatggtgg cccccaatgc
     1381 tccactgata caggcgataa caaaggggta cttacgcggc aggttgacgc catataccgc
     1441 tggttcggtg ataccaaaca gacccgtcaa cgccgctgat cccgccacca cttttttctg
     1501 cgcatcgcgt tcgcagagga agacgccgag cgccgccccg acctgcgcca taatggcggg
     1561 cattaacagc gggatcatgg tgtcgtagcc cagcacggtg aagttattga tacacagcgg
     1621 caccaggccc ctgtgcagtc cgaacatgac gaagatttgc cagaagccgc ccattaccgc
     1681 gcccgcaaat gcaggaaccg cctgataaag ccagagataa ccggcggcaa tcatttcgct
     1741 tatccaggtt gatagctgcc ccaccagcag aaaggtgacg ggtgtgataa ccatcagaca
     1801 tagcaatggt gtgaagaaat ttttgattgc cgacggtaac cacgcattaa gtcggcgttc
     1861 cagaatgctg cacaaccagg cagaaaaaat aatgggaata accgatgacg agtaattcaa
     1921 caatgtgacc ggaataccca ggaaatccag ccccggcgca tccgcttttt gcccgttctc
     1981 gaaggcagtc agaattaatg gatgcactaa cgctccacca atcaccatgg cagtaaatgg
     2041 attaccgccg aagcgtttgc ccgcggtgta tcccaggatt atcgggaaga acccaaacaa
     2101 ggcatcactg gcgctgaata aaattaaata agtaccactt tgttcggtcg tccactgaaa
     2161 agtgagcgcc agagccagca tacctttcag gatcccggtt gccgccatca gaccgatcag
     2221 aggcgtaaaa atacctgaaa taacataaac aaagcggttt agcagattac ctttctcatc
     2281 attttccggt gcctgttgcg ctttttcgcc aaggcctgcc acgctgttaa ccgccaggaa
     2341 gacatcggcc acatggttac ctatgaccac ctgaaactga ccaccgcttt ccaccaccat
     2401 aataataccg ggggtctttt tcagtacctc agcttgcgct ttgctttcat cctttaactt
     2461 aaaacgtaat cgcgttgcgc aatgcatcag actcacaatg ttatctgcgc ccccgactcc
     2521 tgcgactatt tttctggcta actccgtcat aacttgccct ctaccgcttt gcggcaaaac
     2581 tccaaaaaaa aacctgaaaa aaacggcctg acgtgaatca agcaattttt ttcaggtttt
     2641 gcccgcttag tgcggtaaca atcctttact cagtaataat atttcagtgt tctttgcgca
     2701 cgcgctctat atttatggct aaaaacataa tctctgcggg tgaaatttta cgttgatact
     2761 gcaaaccaat aaaaatggcg atccgttccg cacattgcca tgcttgcggg taattttgtt
     2821 ttactgcttg ttgtaatgat tcatcactat cgttaattga agcatgttca agaatacgcc
     2881 aggataaaaa cttcagatgt gtaaccagtc gctgataact caagctttcc tcctggtaat
     2941 taaggctgaa ctgaaatttt attaattgca gcatttcgcg cattaactgc gtgacacctg
     3001 caacatcctc catatttccg ctcatttggg cactgaccag atgcatggca ataaagccca
     3061 cttcatcttt cggtaactgc acgcccaacc gtttatcaat aatggttaac gcttcttccc
     3121 ctagctggaa ctctttcggg taaagccgct ggatatccca cagcaacacg ttttgctgaa
     3181 agcgtttaat cgcaaactgg caatggtcag ttagcgagat ataaatactg tcctgtaatt
     3241 ttcccaagcg ctcctgcgct aaagagataa gacgatcaca ggttgccatc acctcaagag
     3301 gaatatgact taagagttcg cttaatcgcc cgttcagttc atgactgctc aaggcatact
     3361 ctttttctat tccacttgag ttaattcttt cgccagcgcg tttttgaaag ccaattccgc
     3421 gccccatgac gactttttcc cgctgttgat catcaataac caccacaaca ttattgttga
     3481 gaattttggt gatttgcatg ttcatagcaa ggaccttttt tataaacaaa aaaaaaccga
     3541 cttcaccagt attctctggt tatgtcaggt tttgcctgcg aatgcagtaa caatccagtc
     3601 atttattaat ggtttttata acgaacatcc aggttcggaa attaatttaa ttgcgtgcag
     3661 agaaaatagc aatgcgctat tgataaaaat atgaccatgc tcgcagttat taactttgtg
     3721 taattttagg aatttataaa gttatatata acaaatccca ataattaagt tattgggatt
     3781 tgtctggtga attatttgtc gctatctttt cccgccagca gtttatccag ctcatcaccg
     3841 cctacatgac ggaaatcctg acccttcacg tagtagaaga taaactcaca aatattctgg
     3901 cagcggtcgc cgatacgttc gattgaacgc gcacagaaca gcgcggtaag tacgctcgga
     3961 atagtgcgcg aatcttccat catgtaggtc atcagttgac gaacaatacc ttcgtattcc
     4021 tgatcgactt ttttatcttc acgataaata cgtaccgctt cgtcaatgtc catccgcgcg
     4081 aacgcgtcca gcacgtcgtg cagcatctgg atggtatgac ggcccagcga ctccagactt
     4141 accagcaacg gctgatgctg ctgggagaat ttctccagcg cagtacggca gattttgtcc
     4201 gccacgtcgc caatacgctc cagctcggca atggttttac tgatcaccat aaccagtcgc
     4261 aggtcgctcg ccgtcggctg acgtttggcg ataatgcgca cgcaggcttc atcgatcgcc
     4321 acttccatca tgttgacgtt cttgtcgcct tcgatcacgc gcttcgccag atcgctgtcc
     4381 tggttatgca tcgcggtgat tgcatcagaa agctgctgct ccaccatgcc gcccatggtc
     4441 atcacctgcg tgcggatact ttccagttcg gcgttgaact ggccggaaat atgtttatta
     4501 agattgagac tgtccataac gcactcctga atcaaccgta acgaccggtg atgtagtctt
     4561 ctgtttgttt cttcgctggc ttggtgaaca gatcgtccgt gttgctgaac tcaatcaatt
     4621 cgcccaggta cataaatgcc gtgtggtcgg aacaacgcgc agcctgctgc atgttgtggg
     4681 tgacgatcac cacggtgtaa tcctgcttca gttcggtgat cagctcttca atacgcccgg
     4741 tagagatggg gtcgagcgcc gaacacggtt cgtcgagcag cagcacttcc gggcgaatgg
     4801 cgataccacg cgcaatacac agacgctgtt gctgaccacc agagagagag taaccgctct
     4861 ggtgcaattt atctttggtt tcgttccaca atgcggcttt ggtcaatgcc cactgcacgc
     4921 gctcgtccat gtcggcacgg gagagcttct caaacagacg aacgccaaag gcgatgttgt
     4981 cgtagatgga catcgggaac ggcgtcggtt tctggaacac catgcccact ttcgcacgca
     5041 gcagggcgat atcctgagag ttggtcagga tgttgtcgcc atcaagcaga atttcacctt
     5101 ccgcacgctg ctccgggtac agttcaaaca ttttgttgaa ggtacgcagc agcgtcgatt
     5161 taccgcagcc ggacggcccg ataaacgccg taacctggtt tttggcgata tccaggttga
     5221 tgtttttcag ggcatggaat ttgccgtagt agaagttcaa attacgaacc tgaattttac
     5281 tcggggcagt ttcaaccata ctcattgcaa tcttttcctc atcgtacctg atgcttttcg
     5341 ctaatcaggc ttaccgtagg gccgggtaag gcgttcacgc cgcatccgga aaagtgttaa
     5401 ccgtgtttat tcttcgcaaa aacaacgcgc gccagaatgt tcagcagcag gacgcacagg
     5461 gtaatgatca ataccccggc ccaggccagt tgttgccatt ccgcaaacgg gctcatcgca
     5521 aacttaaaga tcgtcaccgg caggttggcg atcggctgca tcatgtccgt gctccagaac
     5581 tggttggaga gcgcggtaaa cagcagcggc gcggtttcac cggcaatacg ggcaatcgcc
     5641 agcaggatac cggtcataat cccggacacc gacgctttca gcgtaatcgc ggagatcatc
     5701 ttccacttcg gtgtacccag cgcataagcc gcttcacgca ggctgtccgg caccagtttc
     5761 agcatgttct cagtggtgcg gataacgatc ggcacctgca acagcgccag cgcaattacg
     5821 cccgcccagc cggagaagtg ctccatctgt gccaccacga tggtgtagac aaacagacca
     5881 accacaatcg acggcgcgga gagcagaatg tcgttaatga agcgaatcac ttctgccagc
     5941 caggatttac gaccatattc cgccagataa atccccgcca taatgcccag cggcgtaccg
     6001 aataccgtgg cccacaaaat taacagcccg ctacccgcca gggcgttcgc cagaccacca
     6061 ccttccgtat tgggcggcgg cgtcatttca gtgaacagcg ccagcgacat accgtcgata
     6121 ccgcgagtga tggtggacat taaaatccag atcagccaga acaggccgaa ggccatcgtc
     6181 gccatcgaga gcgtcagcgc aatacggttt ttgaggcggc gacgcgcctg cattttgcgg
     6241 cgagattcag ccagcgccgc agtggtttgc atttcaacca tagccattag cgtgccccct
     6301 cattcttagc caggcgcata atcataaact tcgatgcggc gaggacgatg aaggtaatca
     6361 caaacaggat caggcccagt tccatcagtg cggcaacgtg cagaccggat tccgcttccg
     6421 caaattcgtt cgccagcgca gaggtgatac tattgcccgg catgtacagc gaggcgctgt
     6481 cgagctggta ggtgttaccg atgataaagg tcaccgccat ggtttcaccg agcgcgcggc
     6541 ccagccccag catgatgccg ccgataacac catttttggt gaacggaaga acgatacgcc
     6601 agataacttc ccaggtggtg cagccaatac cgtaggccga ctctttcatc atcaccgggg
     6661 tttgttcgaa cacatcacgc attaccgccg caatgtacgg aataatcatg atggcgagga
     6721 tcacgcctgc cgcgaggata ccgataccaa atgcggggcc agagaacagc gcgccaacaa
     6781 tcgggatatt cgacatgata ttgccgaccg gctcctgaaa gtaaacggcg aacagcggcg
     6841 caaagataaa caggccccac atgccgtaaa cgatacttgg aatggctgcc agcagctcaa
     6901 tggcgatacc cagcgggcgt ttcagccagc caggcgcaag ctcagtcagg aacagggcga
     6961 taccgaaact caccgggacg gcgatcagca gcgcgataaa cgaagtcacc aacgtaccgt
     7021 agatcggcac cagcgccccg tagatatcgt tcggtgcatc ccactctttg gtccatagga
     7081 aagccagacc aaatttctga atgctcggcc aggaggagat gatcagagag acaataatgc
     7141 cacccaacat caatagcaca atcagcgccg ccagttttac cagcacgctg aaaattatgt
     7201 cgcccttttt acccggtggg ttaaaagcgg gcttggttgc agccataagt tactcttcag
     7261 ttaaacgcgt ttacaaagtc attttgtaat gccggatgcg gcgtaaaaca ccgtacccgg
     7321 cctggagttt tattagtaca gcggcttacc gctactgtct ttaatattgg tcttccacgc
     7381 agcgcgaacc tgttcaacta cactatccgg caggctggcg taatccaggt cgttcgcctg
     7441 tttagccccg gttttgtacg cccagtcgaa gaatttcagc acctctgtgc cttgttctgg
     7501 tttcttctga tctttgtgga tcagaatgaa cgtggtagag gtaataggcc atgcatcttc
     7561 gcctttctgg ttggtcagat cctgagcgaa ggttttgctc cagtctgcac cttttgctgc
     7621 attagcgaag ttttcttcgg tcggactaac cggtttacca tcagcggaga tcagtttggt
     7681 gtacgccagg ttgttctgct tcgcgtaagc atattcaaca taaccaattg cacccggcag
     7741 acgctgaacg aacgcggcga taccgtcgtt acctttaccg cccagaccga tcggccattt
     7801 tacggtagag ccagtaccaa cgttgttttt ccactcttcg ttcactttcg ccaggtagct
     7861 ggtgaagacg aaggaagtcc cggagccatc tgcgcggcgt actacagcaa tgttttgtga
     7921 aggcagtttc agacccggat tcagtttggc gatggcttca tcatcccact tcttgatttt
     7981 gcccaggtag atgtcgccga gggttttacc atccagcacc agttcgccag acttcagccc
     8041 tggaatgtta accgccagca ccacgccgcc aatcacggtc gggaactgga acagaccttc
     8101 ctgagccagt ttttcgtcag acagcggcgc gtcagaggca ccaaaatcaa cggtattagc
     8161 gataatctgt tttacgccac cggaagaacc gataccctgg tagttaactt tattaccggt
     8221 ttctttctgg taagtgtcag cccatttggc atacaccggc gcagggaagg ttgcacctgc
     8281 acctgtcagg cttgcttctg caaacacaga gaaagcactc atcgataagg tcgcggcgac
     8341 aacagttgcg acggtggtac gcataacttt cataatgtct cctgggagga ttcataaagc
     8401 attgtttgtt ggctacgaga agcaaaatag gacaaacagg tgacagttat atataaggaa
     8461 tatgacattt ttatgacaga aaggaattac caaataactt actgatttta aatgtgaaga
     8521 tgaaaattcc acatgccatc tttttgtcat gatgcattta aaagtgataa agaaatgggt
     8581 gagatgacaa cgatgtgatg aacaatcgtt gtcagaccta ttagcgcaca atctcaacag
     8641 ttatcgtcgc tgttgcagta aattgccccg gggctgggcg agctccagta aggctcgcag
     8701 gagcagctga aaaagttaca ctacctgatt tttcaccata cactgttgat ttatccatgt
     8761 ctgttggcaa ctcaccaccg ttcactttga tttccttatt atttttgtcg taaattttta
     8821 tccccacgtt agagtttgaa gttacgacca ttgaattgtc cgtattgggt tcggtagcat
     8881 aaaaagacgt actgatatca tcggtattca gtatccccag gttttcacac tcaatagtga
     8941 ctgttttagt aattttatgt gttgttaccg cacttccaac aacagatgaa aaatctgcag
     9001 ctggtatttc tttaaaatca aaacaaactg gctttaaatt atttatttcg caactttgcg
     9061 gagctgtaat atcgccccga atataaacat cagccatcgg ctgtgagtaa tccaccgggg
     9121 tgtcacggct aattgttcca tacaatgtca ctatctttgt ttcaggaata atcaattttc
     9181 ctgcaaatgt cttcttgata taaa
//
LOCUS       DAJTUP010000132         9156 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTUP010000132 DAJTUP010000000
VERSION     DAJTUP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.132"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..734
                     /gene="hchA"
                     /locus_tag="OO468_003485"
     CDS             <1..734
                     /gene="hchA"
                     /locus_tag="OO468_003485"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR6260465.1"
                     /translation="YPKPYRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYH
                     LHAAGFEFEVATISGLMTKFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNA
                     DSEYAAIFVPGGHGALIGLPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNP
                     LNGYSICAFPDAADKQTPDIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRL
                     TGDSPFAANALGKLAAQEMLAAYAS"
     gene            complement(842..2200)
                     /gene="hprS"
                     /locus_tag="OO468_003486"
     CDS             complement(842..2200)
                     /gene="hprS"
                     /locus_tag="OO468_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR6260466.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2200..2871)
                     /gene="hprR"
                     /locus_tag="OO468_003487"
     CDS             complement(2200..2871)
                     /gene="hprR"
                     /locus_tag="OO468_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR6260467.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3004..3417
                     /gene="hiuH"
                     /locus_tag="OO468_003488"
     CDS             3004..3417
                     /gene="hiuH"
                     /locus_tag="OO468_003488"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR6260468.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3526..4530
                     /gene="msrP"
                     /locus_tag="OO468_003489"
                     /pseudo
     CDS             3526..4530
                     /gene="msrP"
                     /locus_tag="OO468_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4531..5166
                     /gene="msrQ"
                     /locus_tag="OO468_003490"
     CDS             4531..5166
                     /gene="msrQ"
                     /locus_tag="OO468_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR6260469.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5424..6074
                     /gene="zinT"
                     /locus_tag="OO468_003491"
     CDS             5424..6074
                     /gene="zinT"
                     /locus_tag="OO468_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR6260470.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7152..7421)
                     /locus_tag="OO468_003492"
     CDS             complement(7152..7421)
                     /locus_tag="OO468_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6260471.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7478..8146
                     /locus_tag="OO468_003493"
     CDS             7478..8146
                     /locus_tag="OO468_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR6260472.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8462..>9156
                     /locus_tag="OO468_003494"
     CDS             8462..>9156
                     /locus_tag="OO468_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6260473.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2627 a         2062 c         1870 g         2597 t
ORIGIN      
        1 actatccaaa accgtatcgc ggtaaacata aaattctggt gatcgccgcg gacgaacgtt
       61 atttgccgac cgataacgga aaactgttct cgaccggtaa ccatccgatt gaaacgttgc
      121 tgccgttgta tcatctccat gctgcaggtt tcgaattcga agtggcgacc atttccggtc
      181 tgatgaccaa gtttgaatac tgggctatgc cgcacaaaga tgaaaaagta atgccattct
      241 ttgaacagca taaatcgtta ttccgtaatc cgaagaaact cgcggatgtt gtcgccagcc
      301 tcaacgctga tagcgaatat gcagcaatct ttgttcctgg tgggcatggc gcacttattg
      361 gtttaccaga aagccaggac gtggctgccg ctttacagtg ggcaatcaaa aatgaccgtt
      421 ttgttatctc cctttgccac ggtccggcgg cttttctggc acttcgccac agcgataacc
      481 cactgaatgg ttattctatt tgcgcattcc cagacgccgc agacaaacaa acgccagata
      541 ttggctatat gccgggtcat ctcacctggt acttcggcga agagttgaag aaaatgggca
      601 tgaacatcat taatgacgac atcaccgggc gagtacataa ggaccgtaaa cgtctcaccg
      661 gcgacagtcc ttttgcagcg aatgcgttgg gtaaactggc ggcgcaggaa atgctggcag
      721 cttacgcgag ttaatccctg ttacattgtg atgtaagcgc ataatcaatc gcagccacgt
      781 taatactcac gacgtggctg cgttttttca cggttaactt atggcgtact gaagcccgat
      841 gttaatttct ttgcggtaac gtaattcgga acacgttatg cttactgaga tagtgatacg
      901 tagcacttcc tccatgtaat tcggcaatcg ctttgactaa agaaaggcca agtccctgac
      961 ctacggaatg acgcgaatta tctccccgcc aaaatctacg gaagagtttt tcaggctcat
     1021 taattttcgt tccaggactg gcgatatcaa tattaagaga gccgttggca tcaagaaaac
     1081 tggttatatg aatacgcgat ttttctggtg aatatcgaat ggcgttaaca ataagattcg
     1141 ataacattcg ttgcagtaaa attttatccg caaagatttg ttgattgcac ttgaccttaa
     1201 agcaaatctc tttctcgtct gaaagatatt caagatagtc caaaagattt tcaacttcct
     1261 tattgagaga aagcgaatcc agtttcacca aaacattgtt tttgtctgcc ctggcaagaa
     1321 acagtatatt ctctgttaac cgcgaaatat tttccagctc ttcaatgttt ccggcaattg
     1381 ttttttgata ttcagcgata cttctgattt gactgagcgt aacctgattc tgacccagta
     1441 atgcattaat tggcgttcta agttcatgag cgagatcgtc agcaaattga ctaaggcgct
     1501 caaaatcttt gactaaagca tgatgcattt tattcaacgc ctgcccaaga ggttttagtt
     1561 ctctcggtaa tgcgttaacc tcaaccggca ctcggctatc gttataattc agcgcttccg
     1621 ttacgccact caactttttg atctctcgca accccgtcct gattaacaac ggacttaata
     1681 ctgagcacag tataatggcg acaatgcaaa ttataatgct attaatttta tactgttcaa
     1741 gcatattatg tctggctgaa gccaatttag ccacagtaac cgttaatgat ggcgaaactt
     1801 catcaatgtt aattcgtaaa gcatctatct ctgtatcatt aataatgctt ctgtaaatgc
     1861 ctgctgcgct gattgtctca ctagcaggta tgttatttaa catgtcatca ctgacatttg
     1921 tccggttaac aattttattg atgccatcac tatgaatgat caaaatatcc tgactaacat
     1981 ccatcatccg gttaaagtac acaggtaacg tatctggatt taccccatca attaacaact
     2041 gcttgatctg cgctgtccgg ttaatgagtg ttgtatcatc gcgccatttc aactcacttg
     2101 ccaggccatt atagagagtc catacaattc cagcgccagc aacagactgc agaaatataa
     2161 aaagcaaggt taaacggacg gttatagata atcttttcat tattttttta ccgctacgaa
     2221 tgaatagccc atcccccgga ttgtggcgat tagcttttca ggaaaaggat catcaacttt
     2281 tgcgcggagc ctgcgaatgg cgacatccac cgtattggta tcactatcaa agttgattcc
     2341 ccaaatttca ctcgcaataa ccgttctggg tataatctcg ccagctctgg aggccagtag
     2401 ccaaagtaac tgaaactcct tgcgcgtcag tgtaatactg atattgtccc tgctgacact
     2461 ttgactgaca gagtccattt ttaagccact gatttctaat gttgaattca gagcgtgatg
     2521 ttgccttaat tgtgcccgaa cccttgctag caactcagaa aatgaaaaag gttttaccag
     2581 ataatcattt gccccactgt ccaaccctct gactctgtca tcgacagaat cccttgcagt
     2641 aaggcaaata acaggggttt gctttgccgt tcttaacgtt tgtaagatct gccagccatc
     2701 catacccgga agcataatat ccagaatgat caatgcataa tcatccttca gcgcaagata
     2761 aagcccatct ctgccatcag aaacggcatc aatgacataa cccgcttcgg aaagcccctg
     2821 cgttacccat tcctgggtcc tttgattatc ttcaataagt agaatcttca tgaaaataat
     2881 atgccataaa tcatatgtta tagaaacaac ctggttcatt acaaaattgt aatgctgctg
     2941 taaggttacc ctggacgttt tttcgctatc ctcaaaactc attcacatga caaggatata
     3001 aacatgttaa agcgttattt agtactctcg gtagcaacgg cagccttttc attaccttct
     3061 ttggtttatg ccgcacaaca aaacattctt agcgtgcaca ttttgaacca gcaaacagga
     3121 aaacccgctg ccgacgtgac agtcactctt gaaaagaaag cggataacgg ctggttacaa
     3181 cttaataccg ccaaaacaga taaggatgga cgaattaagg cactgtggcc cgagcaaact
     3241 gcgactactg gcgattaccg tgtcgtattt aaaaccgggg actatttcaa gaaacaaaat
     3301 cttgaaagtt tcttccctga gattcccgtt gaatttcata ttaataaagt gaacgagcat
     3361 tatcatgtgc ctttgttact cagccaatat gggtattcaa cctatcgtgg cagttaatgc
     3421 gtaaatatgt aaaagctgat ctctgctact gttagggagg tgactccctg acagattaac
     3481 agtaaacagc tcttgttggt taacgacaaa aaagtgtgat ggcttatgaa aaagaagcaa
     3541 tttttaaaag aatcagatgt tacggccgag tcggtattct ttatgaagcg tcggcaggtg
     3601 ttaaaagcac tgggcatcag cgcagctgca ttttctttgc ctcacgctgc gcatgccgat
     3661 ctgcttagct ggtttaaagg gaacgatcgt ccgcccgccc ccgccggaaa accgctggag
     3721 ttcagcaagc ctgccgccta gcaaaataac ctgccactga cgccagcaga taaagtctcc
     3781 ggttataaca acttctatga attcgggctg gataaagacg atcccgcggc taatgctggt
     3841 agcctgaaaa ccgatccatg gacactgaaa atcagcggcg aagtggcaaa accattgacc
     3901 ctcgatcatg atgatttaac ccgtcgcttc ccgctggaag agcgtattta tcgtatgcgc
     3961 tgcgtggaag cgtggtcgat ggtggtgccg tggattggtt ttccgctaca caaattgctg
     4021 gcgcttgccg aacccaccag caatgcgaag tatgtcgctt tcgaaacgat ttatgcgccg
     4081 gaacagatgc ctggccagca ggaccgcttt atcggcggcg ggctgaaata tccttatgtc
     4141 gaaggattgc gtctcgacga agcaatgcat ccgctcacac tgatgaccgt aggtgtttat
     4201 ggcaaggcgt taccaccgca aaatggcgcg ccggtgcgac tgattgtgcc gtggaaatat
     4261 ggctttaaag ggattaaatc gatcgtcagt attaagctga cccgcgagcg tccgccaacc
     4321 acctggaatc tggcagcgcc tgacgaatac ggtttttacg ccaacgttaa tccgcatgtt
     4381 gatcacccgc gctggtcaca ggctaccgaa cgatttattg gttcaggcgg cattctcgat
     4441 gttcagcgcc agccaacact actgtttaat ggttacgccg accaggtggc atcgctgtat
     4501 cgtggcctgg atttgcggga gaatttctaa atgcgtctga cagcaaaaca agtgatatgg
     4561 ctgaaagttt gcctgcatct tgccggattg ttgccgtttc tctggctggt ctgggcgatc
     4621 aatcacggtg gactgggtgc cgatccggtg aaagatattc agcattttac cggtcgcact
     4681 gcactgaaat ttttgcttgc ggccttgtta atcacacctc tggcacgcta cgcaaaacag
     4741 ccgttattga tacgcactcg ccgcctgtta ggcttatggt gctttgcctg ggcgacactg
     4801 catttaacca gttacgcatt gctggagttg ggcgtgaata atctggcgtt attaggtaag
     4861 gagttaatta cccggcctta tttaacgtta ggaattatca gctgggttat tttgcttgct
     4921 ttagcgttca cttcaaccca gtcgatgcag cgaaaactcg gcaaacactg gcaacagttg
     4981 cataacttcg tctatcttgt cgcgatcctg gccccaatac attatctgtg gtcagtgaag
     5041 attatttcac cgcagccact catctacgcc gggctggctg tactgctttt agccttacgg
     5101 tataagaagt tgctttccct tttcaaccgg ttacgcaagc aggcccacaa caaattatct
     5161 ctataacttc atattgccga caaacttcgc gttatcccgg ccggatgcgg cgcgagcgcc
     5221 ctatccggcc cacacagatc gcatcaagtt atgtttcgtt tgtcatcaac ctttcatcac
     5281 cagaataatt tattttattt caataagata gttcaataca cctgagaata ccatgctctc
     5341 atttcctaat gagttgttgc attttttcat atgttacaat ataacattac acataatgta
     5401 cattaactct ggaggaaatt gttttggcga ttcgtcttca taaactggct gttgctttag
     5461 gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcattctcat ggcaaaccct
     5521 taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgataccaat gtacaaaacc
     5581 gaacgctcag tgactgggat ggagtctggc aatccgttta ccctttactg caaagtggca
     5641 aacttgatcc cgtctttcag aagaaagcgg atgcagataa aaccaaaaca tttgctgaaa
     5701 ttaaagatta ttatcgcaaa ggttatccga cagatatcga aatgattggc attgaggacg
     5761 gcattgtcga attccataga aataatgaaa caacatcctg taaatatgat tacgatggat
     5821 acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc gaatgtaaag
     5881 atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt gcaccacgaa
     5941 aatcatctca ttttcacatt tttatgggta atgattccca gcaatcttta ttgaatgaaa
     6001 tggaaaactg gccaacgtat tatccctatc agttgagtag cgaagaagtg gtcgaggaga
     6061 tgatgtctca ttgatttttt tgcgctgact tcagttattt aacagaacag tgcctggtag
     6121 taacaacaat aatttaccaa gacaaataag taagacaaac tcagacacgc tcatacttga
     6181 gtcgtgtctt ttttattatg tctgtagaaa aaaactgaga aaaaggctaa caataaatac
     6241 acaactcagc catacagact ggcagcgcaa atgtttccag gagcaagtca tctgtcatgg
     6301 taaagctaac cagaaatata ggttaatttt ggtgtttaaa ccttttttac ttgttagtat
     6361 gatacaaagg ctttcaaaaa agctgggaaa ttcaacaact cttggaaatt atggactttg
     6421 tcccgcggct ttaatctgac ggccgcgttc cttttttggc tcaattattg accccttctc
     6481 tacgcaactt cagttcccgc cagcaacttt gcggcagttt cataggatca atgtctaaaa
     6541 gaataatggt gaccgataag aaaacgactg aataactgta gattttcgct cgaaaccttc
     6601 ctgtcagctc catagcgaat caagcgctga atgtcacagt atcgaacaga aaacagtgac
     6661 gatctaaccc ttcaagaata ttctacgatt gttctgttta ggaaaagcaa ggcgggaagt
     6721 cgggagataa gtcattgata aaatggcgga gagaggggga tttgaacccc cggtagagtt
     6781 gcccctactc cggttttcga gaccgatgat acagagtcga aaaatcaaag agttaaaaat
     6841 ttttcttgga aaaaatgtct taaaatcgta cacgaaaaat caatgaatta cataacatta
     6901 aagaagaata ttccaagcct tcttgagtcc accacctgtt ttcaggtgtc attcagacgt
     6961 gatttgcccc agattgggaa agcacgcttt tttctcagct tattccgttt tgcctgtgtg
     7021 ttgaataatt ccactgcagt tatataaaat cggtaacggc tggaaatcat tcaatactcg
     7081 cactatcgaa agtttaccag ccaaccgcag cacgtcctgc atacgtcgtg tctgcggttt
     7141 ttctttttcg cttacattgt gtctggttct tccggccact caatatcagg tgcagttgat
     7201 gtatcaacac ggttcagcaa cacccgatac ttcttccagg cttccagcaa cgagttttct
     7261 tcctccgttg cgatctccag atctacagca tcctgaagtg gcgcaatatg ctcactgaat
     7321 ccctggatgt agaactgtgt ggtgacggtc ttccagccat tcggctcctg ctgtatcgaa
     7381 gcataccagg ctatttcaat atcgctatgc tgcggcagca tttaacccct tgtaattcat
     7441 cgccataatt gatttaattc acaaataaaa ctataatatg gtgaaattaa tgaaaaaaaa
     7501 cacagatgat ggggctaaaa tttacacacc acttacccta aagctttatg actggtgggt
     7561 tttgggagta tcaaatcggc ttgcatgggg atgtcctaca aaggaacacc ttcttccaca
     7621 ctttctggaa catttaggta acaaccatct ggatattggt gttggaactg ggttttacct
     7681 tactcacgta cctgagagta gtctgatatc tttaatggat ttgaacgaag ctagcctgaa
     7741 cgcggcatct acaagggctg gggaatcaaa aattaaacat aaaattagcc atgatgtttt
     7801 tgaaccttat cctgcggcgt tacatggtca atttgattcc atttccatgt tttaccttct
     7861 tcactgcctg cctggaaata tatctacaaa aagctgtgta atacgcaatg cggcgcaggc
     7921 cttaactgat gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa
     7981 tagcttcggt caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa
     8041 agattccgaa gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccaa
     8101 ggttcaaggt actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc
     8161 acgttcttgc ttaagacgtg ctacggtttc ctttacgatt tggggttgcg actttacctc
     8221 tattgataat gcattccggc agaacgttca aatatgaacg tacgatattt aactaaccga
     8281 aaaacaaaat atagcacagg cgagacattt accatctaag aaaatagtat cgttttttta
     8341 cagttaaatc aacatcactt ccttaaaatg aaaacaataa atttcaatcc tgaaacattt
     8401 tatttcagct tattattccc cagtatcaca ttttatcagg atatccttct gcgaggttat
     8461 tatgcttcct gtaaataatc cccccctatc cactggaaac gtctcttttt acagaactac
     8521 atcaatcgac aatgttcaca ataattatct ctccgaatgg gttgaatgga ctaaaaacag
     8581 catttccgga gaaaacaggg aaactgcttt tacccggctc caattatgtc tggagaacag
     8641 tgaaacatcg ttggacttat cttgtttagg tctcagatct ctaccacgat tgcctgacaa
     8701 tcttgatgaa attaatgtaa gcaataacca actatcaatg ctccccgagc taccaagggc
     8761 attgaaagag ctgaatgcaa gcagtaatca attatctgca cttcctgaat taccagtgtc
     8821 gctggaatat ataaatgtga gtgataacca tttgttcgca cttcctgaat tacctgcgtc
     8881 actagaatat attaatgtaa gtgacaatca cctgtctgta cttccgaggt taccaatgtc
     8941 attggaatta cttgatgcag ccagaaatgc tttggaagta ataccagatt ttccagaaag
     9001 agatgatcat attataagaa tattctggct taatcagaac cggatcacgg caattccgga
     9061 aagcatactt ggcctcagtt ctgatagcgt tgtcaatctt agagaaaatc aactatctcc
     9121 cagaataatg caaactttgt tacaacaaac cgccca
//
LOCUS       DAJTUP010000133         9044 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTUP010000133 DAJTUP010000000
VERSION     DAJTUP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.133"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..180)
                     /locus_tag="OO468_003495"
     CDS             complement(<1..180)
                     /locus_tag="OO468_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260474.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLG"
     gene            700..1395
                     /gene="minC"
                     /locus_tag="OO468_003496"
     CDS             700..1395
                     /gene="minC"
                     /locus_tag="OO468_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR6260475.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1419..2231
                     /gene="minD"
                     /locus_tag="OO468_003497"
     CDS             1419..2231
                     /gene="minD"
                     /locus_tag="OO468_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR6260476.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2235..2501
                     /gene="minE"
                     /locus_tag="OO468_003498"
     CDS             2235..2501
                     /gene="minE"
                     /locus_tag="OO468_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR6260477.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2873..3217)
                     /locus_tag="OO468_003499"
                     /pseudo
     CDS             complement(2873..3217)
                     /locus_tag="OO468_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3252..3371)
                     /locus_tag="OO468_003500"
     CDS             complement(3252..3371)
                     /locus_tag="OO468_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR6260478.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3332..3517)
                     /locus_tag="OO468_003501"
     CDS             complement(3332..3517)
                     /locus_tag="OO468_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260479.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3618..3791
                     /locus_tag="OO468_003502"
     CDS             3618..3791
                     /locus_tag="OO468_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260480.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3793..4137
                     /locus_tag="OO468_003503"
     CDS             3793..4137
                     /locus_tag="OO468_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR6260481.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4141..4476
                     /locus_tag="OO468_003504"
     CDS             4141..4476
                     /locus_tag="OO468_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR6260482.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4533..6854)
                     /locus_tag="OO468_003505"
     CDS             complement(4533..6854)
                     /locus_tag="OO468_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6260483.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6971..7180)
                     /locus_tag="OO468_003506"
     CDS             complement(6971..7180)
                     /locus_tag="OO468_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260484.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7931..>9044)
                     /locus_tag="OO468_003507"
     CDS             complement(7931..>9044)
                     /locus_tag="OO468_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR6260485.1"
                     /translation="SQFADTRILVTISDQHIRGALNVPLKGVRYVLRVADDIIGPTAD
                     VMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLIDKGFGILIFILLIASAAAFLL
                     DRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGTLRGVEVLARWKQPHGGYISPA
                     AFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEGFHIGINFSASHIISPTFVDEC
                     LNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNILHENGFVIALDDFGTGYSGLSY
                     LHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLARKLSISIVAEGVETKEQLGYLN
                     QNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2549 a         2106 c         1961 g         2428 t
ORIGIN      
        1 gccgagatat ttccttgtta actctgggga aatgccatct gagttcacac aaatatacgg
       61 gtcgcaaaca acgccgcacg tcggagattg aagtccgcca gcgtcatgtc cagacgccat
      121 tgatgagaac atcatcccca cacctgacaa tccgatataa ataatacgca tcatattcat
      181 agcacatcct gataatccaa ccgtacaaca cttatgctca tttcgtcatc aatccgtaat
      241 caaaaccgct gacctggacg agttacgatg acctgacaga agtatcagac gtcttctgcg
      301 gtttctatga tagagcaatt ctgtggcgga tattaagata acgggtatct gttctttaat
      361 gatgctgcaa aaaatggaaa gttaagaaag cctggcgtgg tgaaaaaata cacttgcctc
      421 aatactggat atttaaacag gtctggagat gctgtcacga ttagggtgcg gaaaaaggaa
      481 gcgtaaataa gaagaatcaa aagtacgacg gcaatgggtt gattgacaag ggtatttttt
      541 aagctatgaa tcagcgccat ttatcacaga atagactttt actctgaata aatgggaggg
      601 tgacttgcct caatataatc cagactataa catgccttat agtcttcgga acatcatcgc
      661 gcgctggcga tgattaatag ctaattgagt aaggccagga tgtcaaacac tccaatcgag
      721 cttaaaggca gtagcttcac tttatctgtg gttcatctgc atgaggcaga acctaaggtt
      781 atccatcagg cgctgaaaga caaaatcgct caggccccca catttttaaa acatgccccc
      841 gttgtactca acgtcagtgc actggaagac ccggtaaact ggtcagcgat gcataaggcg
      901 gtttcggcaa ccggtttgcg ggttattggc gtaagtggct gcaaagatgc gcaacttaaa
      961 gccgaaattg aaaaaatggg gctgcctatc ctgacggaag gaaaagaaaa agcgccacgt
     1021 ccagctcccg caccgcaggc tccagcgcaa aatacaatgc cggtcacaaa aacgcgttta
     1081 atagataccc cggtgcgttc cggtcagcgt atttatgctc cacaatgtga tctgattgtt
     1141 acaagccacg ttagcgctgg ggccgaattg attgccgatg ggaacattca tgtctatggc
     1201 atgatgcgcg gtcgtgcgct ggcaggggca agtggtgacc gggaaacgca aatattttgt
     1261 acgaacctga tggcggaatt ggtgtccatc gcaggtgaat actggctgag tgatcaaatc
     1321 ccagcagaat tttatggcaa agcggcgcga ctgcagttag tcgaaaacgc tttgaccgtt
     1381 caaccgttaa attgatccct ttttaacaag gaatttctat ggcacgcatt attgttgtta
     1441 cttcgggtaa agggggtgtt ggtaagacaa cctccagcgc ggccatcgcc actggtttgg
     1501 cccagaaggg aaagaaaact gtcgtgatag attttgatat cggcctgcgt aatctcgacc
     1561 tgattatggg ttgcgaacgc cgggtcgttt acgatttcgt caacgtcatt cagggcgatg
     1621 caacgctaaa tcaggcgtta attaaagata agcgtactga aaatctctat attcttccgg
     1681 catcgcaaac acgcgataaa gatgccctca cccgtgaagg ggtcgccaaa gttcttgatg
     1741 atctgaaagc gatggatttt gaatttatcg tttgtgactc cccggcaggg attgaaaccg
     1801 gtgcgttaat ggcactctat tttgcagacg aagccattat taccaccaac ccggaagtct
     1861 cctcagtacg cgactctgac cgtattttag gcattctggc gtcaaaatca cgccgcgcag
     1921 aaaatggcga agagcctatt aaagagcacc tgctgttaac gcgctataac ccaggccgcg
     1981 taagcagagg tgacatgctg agcatggaag atgtgctgga gatcctgcgc atcaaactcg
     2041 tcggcgtgat cccagaggat caatcagtat tgcgcgcctc taaccagggt gaaccggtca
     2101 ttctcgacat taacgccgat gcgggtaaag cctacgcaga taccgtagaa cgtctgttgg
     2161 gagaagaacg tcctttccgc ttcattgaag aagagaagaa aggcttcctc aaacgcttgt
     2221 tcggaggata agttatggca ttactcgatt tctttctctc gcggaagaag aacacagcca
     2281 acattgcaaa agaacggctg cagattattg ttgctgaacg ccgtcgcagc gatgcagaac
     2341 cgcattatct gccgcagttg cgtaaagata ttcttgaggt catttgtaaa tacgtacaaa
     2401 ttgatcctga gatggtaacc gtacagcttg agcaaaaaga tggcgatatt tctattcttg
     2461 agctgaacgt gaccttaccg gaagcagaag agctgaaata agcccgctgt aaaaacgcat
     2521 ttatcttcaa ggcagagtca tttctgcctt gagtttttca tccctctcat ccacgttgtg
     2581 gtaaagcggc gagtattctt gctgatactc ctcattgcta tttccacctc cccctttaca
     2641 ccttaaggct gttcccacgc acagaacaaa atgtttatgc ctggttgagt aaataaccat
     2701 gttattagtg tgtatatact gggagatgat aaccccgcaa tttcattatt cattgttctc
     2761 ccataggatg aggtttcccg ccgtaactgg cgggcttttt ttgccccaga attttctctt
     2821 tcaacatcct gtaaacgaaa actgcgccga agcgcagttt aatcgttgat ggtcagaacg
     2881 accagttcac accagccacc gcgttccacg gggactccac accggcacca tggctatacc
     2941 ccaccccgaa atgcccgctt aacgtactgc tgaatgaggc tttaatacct gcctggtata
     3001 ttccacgtct gcccgacaaa tcattgacaa aattaccgtc actattcact ttcacccgat
     3061 tatcatcgac aaattctttg cgcacagccg ccttcaggca gggttcaatt tccataccgt
     3121 tccccagacg catgttgtaa ctcagcgttg cacccagttc acgatatata ctgcgggtat
     3181 cgactgattt cgattccatg ccattggata aatgatagta gcgtctaagt gacctaagct
     3241 ctgctgcaac atcagcccgc aggcaccaga ccaggggatt catcctgaag agacagcgca
     3301 ggtgtattgt gttcaccgct caccaaagac atcatgatga aatgatgata tcccgcataa
     3361 gaaagaggca ttttttacac gcagtgcgct gaagtgtagt tggataaaaa atcactcttt
     3421 tcagataata ctgtcgtacg cttatcttta gacaatgagg ccgtcggaaa aataaaatga
     3481 ctaatgtaat cgttgctgat gaatatcttc tgctcacata aaaatcacac aataatattg
     3541 ataaatcaga ttatcttacc tttacaacat tgcgttaaca gtcaatgaca cttcttgaat
     3601 aaaaatattg tcaatcaatg agttattcct catttaagat tattctcatt cacgtaaaaa
     3661 acataattcc tatcatcaca gccacgctca ttttgaatta tctgaataat agcgagcgtt
     3721 ctttagttaa gcagatctta attgaagacg aaattattgt gtgtgcaact tacttaatac
     3781 cggacatcta agttgaagaa aaaaatactt gccttcgggc ttatctcagc attgttctgc
     3841 tccacgccag cgatagcgga tatgaaccgc accacgaaag gtgcattgct aggcgcaggc
     3901 gttggtttac ttactggcaa tggtgttaat ggcgtactga aaggtgcagc tgttggcgct
     3961 ggtgttagtg cagtaacaga aaaaggccgc gacggtaaaa atgcgcgtaa aggtgcaaaa
     4021 gtgggtgccg ccgttggtgc tgtcactggc gttctgaccg gcaatggcct cgaaggggcg
     4081 attaaaggtg ccgtgattgg cggtacgggt ggtgcaattc tgggtaaaat gaaataagga
     4141 atgatgatga aaaaatttgc tttactggcg ggcctgtttg ttttcgcacc aatgacctgg
     4201 gcgcaggact acaatattaa aaacggtttg ccgtcagaaa cctatattac ttgcgcagaa
     4261 gccaacgaaa tggcaaaaac tgacagcgca caggttgcag aaattgttgc ggtaatgggt
     4321 aatgccagcg ttgccagccg tgatttaaaa attgagcaat ctccggaact gagcgccaaa
     4381 gtggtagaaa aactgaatca ggtttgcgct aaagacccgc aaatgctgtt gatcacggca
     4441 attgatgaca ccatgcgtgc tatcggcaaa aaataatatt tcatatgtac cgcgtcgatg
     4501 aatacgacgc ggttttttag ctaacggcgg gatcagaact tatatttcag gcccaccatc
     4561 actgcggtgt cattgtagcc attatcaccc agctgcacgc ccacattccc ccagacgctg
     4621 gccgctggat tcaggttgcc atttacgcca agtttaattt cccccaaatt actcacccca
     4681 tcttgttcga ctttcacacc gttcattgag acagcaaaat ctttactgtt atggatccag
     4741 ttcatttcaa caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt ctcctgagta
     4801 cgaataaagg tgcggaagcc gagtcgggtc tggatgttgt tatttgcgtc attctcaata
     4861 cgtgagccat tggcttccgt gtgcgcatta tgatcgacgc ccatccagat agcctgtgcc
     4921 tgtggctgga agatccacgt atatttcgcc gcttccagac caaaggattt attcagggca
     4981 aaaccgtaac ccgcttccag cgagacggtt gcacctttcg cgctatagga ttcataggct
     5041 aattcatccc ctttcacgga gtttttaaac cagctatatt gcgcccagga gtcaatgtat
     5101 gcgccttttt tactgatgtc atcggcaaac caagtggcat acagccctgc gctatagcct
     5161 ctgacactcc ctttcgaacg ataatccgac acgctggaat gagttaagtt gtagtcgcgg
     5221 gcataaccag ccatcactcc caaacgccaa ctgtcactat cggtaaaacc accggttaac
     5281 agatcgcccc ctaactgcga gacgtagcga tgcgaggtcg ttctcaactg tccgttgctg
     5341 tcacgccagg cattatgccc gccaatttga cgtagccaaa gtcggcttga acgctcctgt
     5401 tcagttacag gatcgatgta gcgcgtttca cccgcacggt cgttcagacg cataacaaaa
     5461 agagtgttag cggctgccag gttggcgacg taacttccag cttcggggcg caggacgggg
     5521 gtaggcgcaa cagtaggcgc tgaacttcct ccggagtttg tttcaccgcc gctcgttgcc
     5581 tggaagttag tcagatacca gtttttattg gtcgcttcgg tattacctcg ggttaatgaa
     5641 tattcgtagg caccggcgaa aatcctcgaa tccttaataa attccccatt tgattccccc
     5701 tcaacgctga taatctctat cccatttaac gtgtaagctc cgctgcctcc tgcattggta
     5761 actttaactc gggttgttcc agaggtattt ccattaataa cgactttatc cgtagctgaa
     5821 ttatcatcat ccaattcagt tctgagatgc aatacgccat tattaccaac gtaattttca
     5881 gttattgtta atcttgttgg ttcgacatcc cgtccatctg cccgggaaat atagaccgta
     5941 ctattatcaa ccgtgaggtt gctcaccgtt gagtcagctt taatatccca ggtactatta
     6001 tctgaaagcg acagataggt atgagtgtta tcatctgttg aaatattagc tgaacccgtt
     6061 atttttgaat tagttgcatc gacggtcaaa tgacctttaa tattattgat aaaaacatct
     6121 gcagccgatg atacctcact atccgtaatg gtcacgttac tcgtctcatc tttatggtag
     6181 aggatttcat ttgctgaccg tagtgagcta ccagataaaa tatctaccgt tgcttcctga
     6241 ccatttatgg aagactccag cacaattcca gacccctgat gcgcgactgc agatgtattt
     6301 ttcagcgtca ccgtgccgcc attataaatt ttagcaatcg cggcgtcagg acccgttgta
     6361 taaatttctc cctggttaaa tgaaactgtc ccctggcggg cagcgatggc atgagcattg
     6421 tccccatgcg tggtgatatc aacatcggtt aatgtaacgc tggcattggt ataatcaagc
     6481 gtgtcaccag ccttgacgac agtgccatta gcgtacgcgg cataactttc tgttccgtgg
     6541 gttgtgatct ccccttttgc catatccagt tttgctatgg cattattttc attacttcct
     6601 gtatcggcag tactttcttc atcaagagaa gatacacacg cacacgcgct tccgggagat
     6661 gcacacgcaa gaacgccgac tgcgtgatta cccgcagtaa caataaatgt atcatctccg
     6721 tcagaaaaag agggggtaac cgaaccgtca gagttgactc ttattatggt gtcagtattc
     6781 aggtttccac tcgtttgact tagcaaacca attgagtaat cccccgttgt gacaatcgtg
     6841 tttttaccct gcaaaataac agcaccgcca aattcactta cgattgcgct agctgttaat
     6901 gctgggctta gaggactatt gtcagaagga tataaaccgc ccgttgttac aatgacgcca
     6961 ttgttaatgg ttaacttact accctctcct gcaacggata cccgtaactt ccattacccg
     7021 ttgttataac tagcgcatta tcaatatctt ttacttttcc atcttttatt gccacagcat
     7081 gtttatattc atcgtgctga ataaactgtt cagcatatgc attacccacc gccagagcaa
     7141 taagtcccaa actaaacccg gggagttttt tcagtttcat agattaacta tccattcatt
     7201 ggattaacaa acattcctgg tcaaataacc tatgagctca gattaccaaa acggagggta
     7261 aagacaaaaa caaaagcaca aaaaatttaa atagataaac agcgacaata tataaatgat
     7321 aaataagtcg gcattatcat ttttcacctt attttcatga cattgatcac tttggggtga
     7381 ctattttgtt taacaaataa ctcttcgaca tcaaaattat taatagttgc catggtaatt
     7441 atttcggtga tatataaaaa caactgtgat aacaccaagg ttgacaacta tttgcgcaat
     7501 aattcctttc cttcaatgat ccaggcgttc actgccagca acccattcag ccgcatactg
     7561 acagtgagaa actgaaagat tattgctgat ccctgtggct aacgagtctt tctcttaaat
     7621 cgattaacca gattgatcgc cagccaaaca agggcattaa gcaaatttat cgtcacaagc
     7681 acttctccca gcccggtact gggttttgca ataaggatga ccaggagggt gatcagcata
     7741 aatgcaatgg aaaagatgat atagagagtg aaataatcca gattattact attattcatg
     7801 ctactaatgg caatcctgtc aaaactttca aacaattata gcaaacaaaa taatgaacaa
     7861 tagtaacaag aggcaacgag ctaattagct gtctataatt acaccgcact atggccttac
     7921 tgatcaattt tcactcaacc acaaccttca ccttcggttt agaaaggata attttgacca
     7981 ggtcgatgta tgtaacaggt ttatagaaat aataaccctg ctgaaatgtg atattatttt
     8041 ggttcagata gccaagttgt tctttcgttt cgacaccttc agcgacgata ctgatcgaaa
     8101 gtttacgcgc cagatccaat acacaatcca gaattcgggt tgattctggg tctgcgttta
     8161 cgcggccaac gaaactgtga tcaattttga tataatcaat atgcaggtca tgaagataag
     8221 aaagccctga gtagccagta ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca
     8281 gaatgttcaa ccgctgaacc agactttcat caacattcaa tggctcacgc tcggtgactt
     8341 ccagaacaag gtttaaatcg cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga
     8401 caaacgtcgg cgaaataata tgcgaagcgc taaaattaat tccaatatga aaaccttccg
     8461 gcagtttact cgcgatagcg ttcatctgtc tggcaacctg attcatcagg ctttgtgtaa
     8521 gcggaacgat taatccggat ttttcagcaa gtggaataaa tgccgcgggt gaaatatatc
     8581 caccgtgagg ttgtttccag cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac
     8641 catttaccac aggttggtaa aaagggacga tctccccatt atttattgcc cgtcgcagga
     8701 tctcttcagg cgttgcgctt ttattgaaat atctatcaag caggaaggca gcggcgctgg
     8761 cgattaacag aataaatatt aaaatcccaa agcctttatc gataagtctg tagaagctaa
     8821 agagtggcgg cgggttaaag ataatagtga aatgatattt tgtggagtga accttctcgg
     8881 tatagggata atgtccatta agcgtcatca catcagccgt tggtccaata atgtcatccg
     8941 ccacgcgtaa tacatacctt acccctttca agggtacatt aagcgccccg cgaatatgtt
     9001 gatcacttat cgtaaccaaa atgcgcgtat ctgcaaattg gctc
//
LOCUS       DAJTUP010000134         8997 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTUP010000134 DAJTUP010000000
VERSION     DAJTUP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.134"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..187)
                     /locus_tag="OO468_003508"
     CDS             complement(<1..187)
                     /locus_tag="OO468_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HCR6260486.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAID"
     gene            complement(184..1062)
                     /gene="xdhB"
                     /locus_tag="OO468_003509"
     CDS             complement(184..1062)
                     /gene="xdhB"
                     /locus_tag="OO468_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR6260487.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1073..3370)
                     /gene="xdhA"
                     /locus_tag="OO468_003510"
                     /pseudo
     CDS             complement(1073..3370)
                     /gene="xdhA"
                     /locus_tag="OO468_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            3785..4540
                     /locus_tag="OO468_003511"
     CDS             3785..4540
                     /locus_tag="OO468_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6260488.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4619..4692
                     /locus_tag="OO468_003512"
     tRNA            4619..4692
                     /locus_tag="OO468_003512"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4651..4653,aa:Gly,seq:ccc)
     gene            4827..6437
                     /locus_tag="OO468_003513"
     CDS             4827..6437
                     /locus_tag="OO468_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6260489.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6469..7076
                     /locus_tag="OO468_003514"
                     /pseudo
     CDS             6469..7076
                     /locus_tag="OO468_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8007..8753
                     /locus_tag="OO468_003515"
     CDS             8007..8753
                     /locus_tag="OO468_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6260490.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2302 a         2206 c         2309 g         2180 t
ORIGIN      
        1 tgtctattgc tgtgccgtcg accaacaccg tacaggcacc acattcaccc acgcagcacc
       61 cttgtttgac acttagcagt ccttgttcgc ggagtaattc cgagagcggc gtgcctggtg
      121 cggcgtgaag ctgaaaaggc atcccgttaa tggtgcattc ggtggtaatt gtttcgctgt
      181 gattcattgc aattttcccc ccgccgcggc gacggcttcg ctaatcactt ttttggtcat
      241 cgtctggatg agatgcagac gaaactcttt actggcccgc catgaagaac gcggggcgac
      301 atcttgcagg acagattcgc tgatagcttc cagcgtttgc aggtttaatg gcgcattttg
      361 tgcagtctgt tcggcatgtt ggcagcgaat cggcgttggc gcggcaacac caaatgccag
      421 gcgtaattcg ctgaaattgc cgttatccag tcggcaatgt gcggcgcagc caatcgttga
      481 aatatccatt gcgtcgcgca tggcatattt aaaatgcgcg ctgcccgcgt gttctttcgg
      541 ctgtggcgga aaatgaaagg cgacgaggat ttcgtcatgc tcaagagaca ctttgcccgg
      601 cccggtgtga aagccattaa tcgggacgaa acgaacaccg cgcggggagt ggatctccag
      661 tttcgcgtca taaattagcg ttggcgtggc agaatctgcg ctggtggcac cgttgcaaat
      721 atttccaccg taggtagcga cgttacggat ctgcggtcca gcaatgaacg tggccgcagc
      781 acataacgcc gggagatgac gttgagttat aggatcttct attagctggg taaatgtcgt
      841 tgcagagccg atacgtagcg agccatcttc cgccagcgta attccccgca gctccgccag
      901 attatgaata tcaacaatat ggcgataacg gtcattgtga tggtggagct gaatcagtac
      961 gtcagtgcca ccggcgagca gtttggcctg cgggttgtca gccagcaggt tgatggcatc
     1021 ggcaagggtt gctgcgcgat ggtaagaagc aaaatcaaac atgatgttat cctcaaatca
     1081 atcctgccag atggaactct tcatataacc gttttggcgt cagcggcagt gtattgattg
     1141 caacaccggt agccatcttc accgcgttac gaatagcagc ggcaacagga attatgggtg
     1201 gctcacccag tgacttatgt ccgtatgcgg attgcggctc attgatttcg acgaacgcgc
     1261 tttccagttg tggcagatcc ggcatggtcg gcattttgta atccagcaga ttggggttac
     1321 ggaccacgcc gcttttagca tcgatgatca tctcttcaaa tagcgcccag ccaatgccca
     1381 ttcccattcc gccgtgtacc tgaccttctg ccagcagtgg attaagaata tgccctgaat
     1441 catgaacgtt gaggatgcgg ttgatggtga ctttgcacag cgcaatatcg accgtcagat
     1501 caacaaaggt acagccaaac gccggtgggt tagtggtggt tttgatggag ctttcagcag
     1561 agagctgccc gccgcgttca gggtggtaga aagcgtccat cgccaaatct tttaacgaca
     1621 ttaacggctc ttccggtcgt tcaaccagca cgatatggcc ttttatcagg gtcaaattca
     1681 tcgctgactg atgtagcatg actgcggcgt gagcgatgat tttctctttt aataatagtg
     1741 ccgcactgcg cagcgcaggc gcggcaacat agctctggcg tgaggcaaat gcgccgggat
     1801 cgaacggcgt aacgtcggta tcttgtgttg aaataacgcg aacgtcgctg accggaaccc
     1861 ccacggtttc tgccaccatt tgcgagaaga cggtgtcggc accctgaccg atttccgtcg
     1921 cgccgctttg cacgttgatg gttccatcct gattcatcag aaggcgcgcg cctgctattt
     1981 ctacgccgac aggccaggtg ttagaggtgt agctaaaaca ggcgacgcca acgccgcggc
     2041 gcaaattgcc ttgctggttc tggcattctg cacggcgttt ttcccattca aagattttcc
     2101 ggcctttttc aagacactcc ggcaaccctg cgctgcaaat acgtttgccc gtgagcggat
     2161 tagcatctcc ttcgcgggcg gcgttgcgta aacgaatttc aacaggatca atacctaacg
     2221 ctgtcgcggc gtcatcaagc atagactcaa cggcaaatac gacttgtggc gcgccataac
     2281 cacgcatcgc accagccgag gggaggttgg tatagcaggt ctttgaactg taagcgtagg
     2341 cacaacgagg ataaaggtaa gcgactttat tcccccccgc agaagcgatg gagtgcccgt
     2401 gagatgcata agcgccggtg ttagacagaa catccagact ataacctttc aaggttccgt
     2461 cgcggttcac gcccatttgc ccgtcaatag taaaagcgtg gcgggtacgg gttgcgagga
     2521 aacactcttc acggctaagg gaaactttca ccggaatgcc gccaagcttg ctggtcagga
     2581 atgccgccat tggctcttcc agtacatcct gtttattacc aaaaccgcca ccgacaaacg
     2641 gtttgatgac tcgtacgcat gaccagggaa tatccagcgc ctgaccaacc acgcggcgaa
     2701 caatgtgcgg gatctgggtg ctggaaacga tggtaattcg cgagtcatcc tccatccacg
     2761 ccagcgatgt tacgctttcc atgtgacaat gttgaataac gggagtctga tagtgcccct
     2821 gtacctggta gtcggcggca tcgattgttt gttggacatt gcccgtcgac atcgtgcttt
     2881 gtttcagtaa attgccgccg ttatggattg gtgcagcgtc ttctgtcagc gccgcttctg
     2941 gcgtggtgat aacgggtaat tcttgccact caatgctgac caattgcgcc gctttttctg
     3001 ccgtgagttc atcgcgggcc acgacgatgg caacggcgtc accatgatga cgaacatggc
     3061 gagttagcag tgcgcgatcg gcggtatcgc gcttgttttc gtcaagtgtc taggcatgcc
     3121 cagctgtagc gaatggaata tcaggcacat cttcccaagt aaaaatcgcc agtacgcccg
     3181 gtaaacttct ggcttgttca tcattaatac ttacggcata accatgtgcg atagggctac
     3241 gtacatattt cgcgtaacac atgcccgcca taacataatc gtcagtatat cgtgcccgcc
     3301 cggtgacctt agcaatggca tcgacgcgca tgcatgattc acccgtagcg gttgcttccc
     3361 gcgcttccat agaaatcccc tcgatttgaa catttggttc ttatggcaaa gatttagtct
     3421 aagcaggcaa gatttacgcc agaaacgacg ataagcgtta ggggaggaag gaagtgtgat
     3481 gacgttaaaa aaacacagat atgatacgaa acaggactct gaatagggac agtgatacgt
     3541 atcaatatga gtggcgataa aatcaatgtg ataaatcagg attattacgc atcaggatgg
     3601 gaaatagggt aaaaagtcag gaacttcgat acctctacag cgaatatttt gctgatgata
     3661 cttccctttg ccaaatgaaa ttaacctctt tctctattac cacgtttttc cagaagcaag
     3721 agattgggta tcaagaggct ggctgctatg atatggcgtc ttgtttttta agcgaggaaa
     3781 gattttgagt gcgggacgcc tgaataaaaa atctctgggt atcgtgatgt ttttatcggt
     3841 tggactgcta ttggcgggct gttcgggtag caaatcatcc gatacaggaa cgtattccgg
     3901 ctccgtttac accgtgaaac ggggggatac gctatatcgt atttcgcgca ccacgggaac
     3961 cagcgtaaaa gagctggcgc gactgaacgg catttccccc ccttacacca ttgaagttgg
     4021 tcagaaacta aaactgggtg gggcgaaaag tagcagtagt acacgtaaat caaccgccaa
     4081 atcaacgact aaaaccgcat cggttacacc gtcatcagcg gtaccgaaat cttcctggcc
     4141 gccagtaggg caacgttgtt ggttatggcc aacgacaggg aaagttatca tgccgtattc
     4201 gacagcagat ggcggcaata aagggattga tatctcagct ccacggggta cacctattta
     4261 cgccgcgggt gcaggaaagg tggtgtatgt gggcaaccag ctgcgtggct acggtaatct
     4321 catcatgatt aaacacagtg aagattacat tacggcttac gcccataatg acacgatgct
     4381 ggtaaataat gggcaaagcg tgaaggctgg gcaaaaaatc gccactatgg ggagcacgga
     4441 tgcggcatct gttcgcctgc atttccagat tcgttaccgt gcaacggcaa ttgatccgct
     4501 acgttacttg ccgccgcagg gcagcaagcc aaaatgctga tggcgaatta atcagcagtc
     4561 agcagcgcgg cacttgctaa ggagagcgta aggtttataa tgccttacgc atctcgaagc
     4621 gggcgtagtt caatggtaga acgagagctt cccaagctct atacgagggt tcgattccct
     4681 tcgcccgctc catgacactt cttaaagtct actaacttac tgaatcctaa gggatacggc
     4741 ttaacgcctt ccgatagttt tactcactgt aaccaacact gtgactaact ttgttgaagt
     4801 agtgagcaga cctaaggtct cagtacatgc tcaagtcaag aacctacctt tatcagcgta
     4861 atggtgtctt ttacatccgt cttaggatga agaccactag tcgcctgacc gcttcgcttc
     4921 cttctcacaa ccgttataaa ctagcgtcag tatctttacg gaccaaggat agacgtaccg
     4981 ctatggctca ctcacggcac atcaagtcag cacttaaagc aatccatgca gacaacccta
     5041 acgcctctta tgaggagcta cgggagcacc tgaagaccat tgtcgagtgg gaacttagtg
     5101 tcagccgtga tgacctgaac gacccggagt cctaccagct ttacgttgac cagtacgatg
     5161 acattaagtc caaccttcgg gaagctgtag cgaccgagcg tctgactgta gaccagcatc
     5221 gctatattaa tgacgttatc ggtgtgctta aggcctgtca ggatagactc aatggcgata
     5281 gctctggcct attgtcttac cttgagcctg agactggcag tctaagaccg tccgtctccc
     5341 tatctgtatt ggcggaacct gaagtccctg aacctaaagc actgaccctc gcgtctctca
     5401 ttgagcagta cgagcaggag aacgcccaga actggaagcc agcgacccta agcgaaaacc
     5461 gagcgtcaca ctctacgcta atcgagatat tcgactatct ggatattcag gatgtcggca
     5521 aggcgacccg tgcagatatg ctcagagttc gtgaagtcct ccagcagcta ccgaagaacc
     5581 gcaagcaacg ctttaagtct atgccgctgt ctgacttgct gaaccgggag tctaagactg
     5641 actgtctgga cgtcgttacc attaacaata aatatcttat caagatggct gctgtgttta
     5701 agtgggcggt acgcaacgac ttgatagcta agaacctgac cgaaggtctt gagctgaaag
     5761 tccctcagcg taaagcctcc gacgctcgtg atgcgttcag tcctgagcag gtagggcaac
     5821 tactggtcgc agctaaagcg tactcgcaga agacctcagg taagccatac cattattacg
     5881 tcaccgctct ggctgcgatt acaggtgcga gacttaacga ggtggcccaa cttcaggtta
     5941 aagatgtcag gaccactgag gctgggaccg tttttatcca tatcaacgaa gacgatagca
     6001 gcctgccggg taagagtatc aagaacgccc acagtgaccg ctgtgtcccg ttggtagatg
     6061 gtgcttatgg ttttgtattg gctgacttta tgtcactcgt ggaagaccgt agaaagactg
     6121 agggtgataa tgctatggtg ttcaatggcc tcaagctaat gaagaacggc tacggtgagc
     6181 aggttagcaa gtggtttaac cgaacgttgc tgcccaaagt cctcgctgac cgtagcggct
     6241 tagcttttca ctcgtttaga catacagttg cgacccagtt aaaacaacat ggggtcgagt
     6301 tagcgtatgc tcaggcgatt atgggtcata gctcagggtc tataacttac gaccgttacg
     6361 cgaaagaggt cgaggtggaa acgctgaaag aaaaattagc ggagtcattg tcagtaaaaa
     6421 aaatagacgg aaagtaatcg catagggata gcggaatgtg aggacgtaat ggtcaaagaa
     6481 tttttagtta gcaaagaaaa taagccttca tatgaatgta tgaatactgc tgaaacttgt
     6541 ttggggctgg ctggaatggc ttcggaagat tggcaacgtg agcatcattg ccgcacaggt
     6601 ataatattta ctgcttttgc tattgaggca atgttcatct tctatagaaa acaggttgac
     6661 cccggctacg ataagacgca gaaagagtgc agaaagacaa tgcacaagaa tacgttaaaa
     6721 ttgtgcggta taaacaatta tatggggaca aaaccttacc aaattatcaa agagtgtctt
     6781 gaggtaagag acgctattgc tcacggcgat tcgtatactt cgagctttaa tttctcggcg
     6841 gatcatttgg ataaccaaga cgatattgca aaagtgtcct ggcggtaaac tctgaacaat
     6901 ttcggggatt aactgttgag cgccttgcaa aatacattga aatggcaaag catattgacc
     6961 atgagatttc tgataatggt tacaggcctt ctgaaggtta ccttccagca gccgagcgca
     7021 gaagtctgat gttagctttt ggcgtcagtg gtgtgtcaat gtggcccagt gcttgagcga
     7081 taatacacct cactatagag gaatggaccc ttaactatca ctatagggga gagaccctaa
     7141 gtccccacct taatagcagt cacgacccat acacacattc attaagtctc aggcttccgg
     7201 tagcggaccg attggaaacc atccgcagac gcctagtgtt aaatcacctt ggcctgagac
     7261 cattcctttt aacgtaagct atcttccctt ttgacaggcg gggagcagta gaccacatcc
     7321 gactttaagt tggctctgtg ataatcgctg cgcggataac tgaagaacca agacaccatt
     7381 actgaggtct gccatccatt tgtggacagg cggaaactca aagaggttag atcagtggtg
     7441 tctgaggttg gagctttgag tcttgaccag caggctggag gagaccatga gtttatcttg
     7501 ttatggattg tgttatcaca attccaataa gaagaacttt aagtccagcc tccactcccg
     7561 ccaacccgaa gtaaaattat ggtcgctatg atgtggtctc catgcttcgg gtcttactgg
     7621 aagtactgaa cactttcgcg tcaagttctc cgaagttcat tcctgtttga ctaaactatc
     7681 actataggga ccatatctcg cctctgacct cctgagagac ggcaagcgag aaaccgtggt
     7741 ccacgctagc aagctagcca ccagcctaag gcaagacagc ctctggtcag cctgtgtgtt
     7801 ctttggtctc gtgacttaag tctatcacca cacacgctcg gacatgctgt cgaaccttca
     7861 tctctatggt catcgcagac cactaacccc aattacctca agtcattctg tttgcgtcct
     7921 gttgcctgct ggtctccctt tacgtggggg cttgagtagg tcagtggggt gcgacttcag
     7981 ggtgcaaaca aaaggagtac acgtaaatgt cccaacaaga aatcagcatc attaacctcg
     8041 accagcttgt gtccatgacc tcagttgaaa ttgctgagct gacaggaaaa gaacataaac
     8101 acgttttacg agatattcgt aatatggtcg aagagcttaa cggggccaaa actgagcact
     8161 gctcaacttt gagcagcgag cttaacgggt ccaaatttgg gctcgttggc gaggaagtat
     8221 acaaggacgc gaaaggtgag agccgaacaa tgtatcgtct cgaccgtaag catactttca
     8281 ttctggtcgc tggctattcc gttcacctcc gcgccaagtg ttacgaccat attcagacgt
     8341 tagagcgtcg agtccttcaa ctggaagacc agaagaagcg agcagccatt cagtccgcta
     8401 accgccgagg cgtgacttgg ggcgactact gcaaaaccta tggtctgccc gctcagaagc
     8461 taatgaccgc acttctccag catcgtggcc tctttcgtaa gaacccaatc agtaacgagt
     8521 ggtctgtaaa tccgaagtat tccgactgct ttcgtatcat taagccgtca gaccagaagt
     8581 tctcagcagg tgggtataac ttccggttca acgctaaggg gctggaggtc ttcggtaagc
     8641 ctgagatggt tgacaagatg cgaggcatcc ttattgcatt cactggcacc gaccagcaga
     8701 agcaagagca cctcctgaag ctggcacaat ccggtaaggt ggagggaatc taagatgatg
     8761 attgacccgc gcacacctga ggggcgcatg actctccgtt accgtggcta ccggactgaa
     8821 gtcctcctca gagagcttgg tctagacccg gaagatgaga cccgccagca ccaatcccga
     8881 gacgagctaa tagcccaact ggtcgctatg aaactgccgc ttaaccgtta gtagctcacc
     8941 tactcaatac ctaatttaaa caccttttga gatgaccgat gcggggagac ccgtagg
//
LOCUS       DAJTUP010000135         8914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTUP010000135 DAJTUP010000000
VERSION     DAJTUP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.135"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(374..1351)
                     /gene="epmA"
                     /locus_tag="OO468_003516"
     CDS             complement(374..1351)
                     /gene="epmA"
                     /locus_tag="OO468_003516"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR6260491.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1676..3484
                     /gene="frdA"
                     /locus_tag="OO468_003517"
     CDS             1676..3484
                     /gene="frdA"
                     /locus_tag="OO468_003517"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR6260492.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3477..4211
                     /gene="frdB"
                     /locus_tag="OO468_003518"
     CDS             3477..4211
                     /gene="frdB"
                     /locus_tag="OO468_003518"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR6260493.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4222..4617
                     /gene="frdC"
                     /locus_tag="OO468_003519"
     CDS             4222..4617
                     /gene="frdC"
                     /locus_tag="OO468_003519"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR6260494.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4628..4987
                     /gene="frdD"
                     /locus_tag="OO468_003520"
     CDS             4628..4987
                     /gene="frdD"
                     /locus_tag="OO468_003520"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR6260495.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5050..6182
                     /locus_tag="OO468_003521"
                     /pseudo
     CDS             5050..6182
                     /locus_tag="OO468_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            6271..6804
                     /gene="blc"
                     /locus_tag="OO468_003522"
     CDS             6271..6804
                     /gene="blc"
                     /locus_tag="OO468_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6260496.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6801..7118)
                     /gene="sugE"
                     /locus_tag="OO468_003523"
     CDS             complement(6801..7118)
                     /gene="sugE"
                     /locus_tag="OO468_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR6260497.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7294..7440)
                     /gene="ecnB"
                     /locus_tag="OO468_003524"
     CDS             complement(7294..7440)
                     /gene="ecnB"
                     /locus_tag="OO468_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR6260498.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7551..7676)
                     /gene="ecnA"
                     /locus_tag="OO468_003525"
     CDS             complement(7551..7676)
                     /gene="ecnA"
                     /locus_tag="OO468_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR6260499.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7728..8294)
                     /gene="efp"
                     /locus_tag="OO468_003526"
     CDS             complement(7728..8294)
                     /gene="efp"
                     /locus_tag="OO468_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR6260500.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8336..>8914
                     /locus_tag="OO468_003527"
     CDS             8336..>8914
                     /locus_tag="OO468_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HCR6260501.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRI"
BASE COUNT         2256 a         2307 c         2278 g         2073 t
ORIGIN      
        1 ggaataaaga ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag
       61 taataagccg atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt
      121 cctatcagac tcatcttttt tatcgtgtga ggcatgaaac cgtccttcat ttcagcgcgt
      181 gtataacacg tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa
      241 tacagaattg atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga
      301 aaaatgaaaa catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag
      361 taatttttca gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg
      421 cgcccagcgc caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca
      481 taccgacttt caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg
      541 ccgcgcgctt acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt
      601 catggaaacc attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc
      661 gatgatcttc ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa
      721 aggtcggttt ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca
      781 acgtgtcgcg gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt
      841 cccgcagttg cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt
      901 gttgataaga aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca
      961 cctcgttcat caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact
     1021 cagggttgtg ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg
     1081 gcccacaacc agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga
     1141 gattcatccc ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa
     1201 tatcggttac cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg
     1261 caaagaaacg acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg
     1321 cgctcggctg ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca
     1381 cgaagtctac tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg
     1441 gcagtgacta aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga
     1501 taatttagcc ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc
     1561 agactatact gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc
     1621 aggcacttac cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca
     1681 aacctttcaa gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat
     1741 tgctgccgcg caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat
     1801 gcgtagccat accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag
     1861 cttcgaatat cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt
     1921 cgtggattat ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg
     1981 cccatggagc cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat
     2041 cgagcgcacc tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca
     2101 gacctctctg caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct
     2161 ggttgatgat ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt
     2221 gcagatccgt gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta
     2281 caacaccaac ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt
     2341 tccgctgcgt gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat
     2401 cctgatgacc gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg
     2461 ttatctgcaa gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata
     2521 tatggaactg ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg
     2581 caacaccatc tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga
     2641 gaaaaaactg catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt
     2701 cgatccggtt aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat
     2761 cgaaaccgat cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc
     2821 ctctgttggt ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt
     2881 cttcggccgt ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa
     2941 cgaagcggca attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa
     3001 ccaggatggc ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga
     3061 aggttgcggt atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga
     3121 gctgcaggaa cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga
     3181 cctgctctac accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc
     3241 cgcaatggca cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga
     3301 gcgtgacgac gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac
     3361 tcgcctggag tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg
     3421 tggcgaagcg gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg
     3481 ctgagatgaa aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac
     3541 cgcatagcgc attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg
     3601 gctacatcaa agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga
     3661 tttgtggttc ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc
     3721 tgcgtgatta caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg
     3781 atctggtggt cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca
     3841 tcggcaactc ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa
     3901 agtatcacca gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt
     3961 ttggcctgaa cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg
     4021 aagatagccg cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg
     4081 tatggagctg tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg
     4141 ctgcggccat tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga
     4201 aaccacgcta aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg
     4261 tccacctggt ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg
     4321 gttccggctg tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc
     4381 ccggaagcct gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac
     4441 ctgatcactc tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa
     4501 gcggccaata tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc
     4561 tgggcggtaa ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga
     4621 gcctgagatg attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt
     4681 cggggccggt ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat
     4741 tctgctgcca ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt
     4801 cgcgcagagc ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg
     4861 tggtttacac cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa
     4921 atgggttttc tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac
     4981 aatctaacgc atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa
     5041 ccagacctta tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca
     5101 tttgctgccc ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag
     5161 caacaaaaga tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt
     5221 acctggggct atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag
     5281 ttaggttcgg tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg
     5341 gaaatcaagt taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg
     5401 aatgggatca cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg
     5461 ccggatgaag tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca
     5521 tgggctccag gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg
     5581 gctgtgaagc cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca
     5641 ctcaaactca accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg
     5701 ggatatcgcg aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat
     5761 ggtgtgaagt cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccttg
     5821 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5881 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     5941 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6001 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6061 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6121 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6181 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6241 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6301 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6361 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6421 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6481 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6541 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6601 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6661 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6721 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6781 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6841 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6901 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     6961 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7021 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7081 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7141 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7201 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7261 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7321 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7381 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7441 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7501 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7561 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7621 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7681 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7741 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7801 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7861 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7921 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     7981 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8041 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8101 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8161 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8221 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8281 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8341 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8401 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8461 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8521 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8581 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8641 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8701 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8761 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8821 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8881 ggaagccatc ccgcatatca aacgtctgcg gatt
//
LOCUS       DAJTUP010000136         8849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTUP010000136 DAJTUP010000000
VERSION     DAJTUP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.136"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(71..1486)
                     /gene="phoA"
                     /locus_tag="OO468_003528"
     CDS             complement(71..1486)
                     /gene="phoA"
                     /locus_tag="OO468_003528"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR6260502.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1587..1847)
                     /gene="iraP"
                     /locus_tag="OO468_003529"
     CDS             complement(1587..1847)
                     /gene="iraP"
                     /locus_tag="OO468_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR6260503.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2029..2232)
                     /locus_tag="OO468_003530"
     CDS             complement(2029..2232)
                     /locus_tag="OO468_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260504.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2310..3404
                     /gene="ddlA"
                     /locus_tag="OO468_003531"
     CDS             2310..3404
                     /gene="ddlA"
                     /locus_tag="OO468_003531"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR6260505.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3428..3640)
                     /locus_tag="OO468_003532"
     CDS             complement(3428..3640)
                     /locus_tag="OO468_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR6260506.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            3900..4208
                     /locus_tag="OO468_003533"
     CDS             3900..4208
                     /locus_tag="OO468_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR6260507.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4267..5361)
                     /gene="yaiW"
                     /locus_tag="OO468_003534"
     CDS             complement(4267..5361)
                     /gene="yaiW"
                     /locus_tag="OO468_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR6260508.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5374..6594)
                     /gene="sbmA"
                     /locus_tag="OO468_003535"
     CDS             complement(5374..6594)
                     /gene="sbmA"
                     /locus_tag="OO468_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR6260509.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            6946..8103
                     /gene="ampH"
                     /locus_tag="OO468_003536"
     CDS             6946..8103
                     /gene="ampH"
                     /locus_tag="OO468_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR6260510.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8104..8607)
                     /locus_tag="OO468_003537"
     CDS             complement(8104..8607)
                     /locus_tag="OO468_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6260511.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(8695..>8849)
                     /locus_tag="OO468_003538"
     CDS             complement(8695..>8849)
                     /locus_tag="OO468_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6260512.1"
                     /translation="NGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANV
                     GVKYTW"
BASE COUNT         2204 a         2264 c         2105 g         2276 t
ORIGIN      
        1 ctgccattaa gtctggttgc taacagcaaa aaaaccaccc ggcagcgaaa attcactgcc
       61 gggcgcagtt ttatttcagc cccagagcgg ctttcatggt gtagaagaga tcggtctggt
      121 cggtcagtcc aacaacattg gcggcatgcg ggccatacgc cgcaatacgc aactgactgc
      181 cggtatgttc ttgtgaatcc tcttcggaat tcccgtaact catcaccatc actgcgccat
      241 ctttagtatt tagcgcctga gtgaggcccg gcgctttggt atccggcgca acaacctggc
      301 tggcgtgggc gtgatcagcg gtgactatga ccagcgtgtt accctccttt ttagcgaatt
      361 ccagcgcccg ttgtacggct tcatcgagat cgaccgtctc gccaatttgc ccacaagggt
      421 tcgcagcgtg atcctgttta tcgattgacg caccttcaac ttgcaggaaa aatcctttct
      481 catttttact caacaattca atggctttgt cggtcatctg tgccagggtt ggtacactgt
      541 cattacgttg cggattaggc gtataggtga ctgcgggctt atcgatattg ccgtggtacg
      601 ttgctttcgg ccctagccag cgcactggca tattgccgtc agcaaacagg ccaagcaggg
      661 gtttttgctg attcgcttcc gtcaccgaat tcagtgaggc agcatcgttc accaactgat
      721 aaccacgcgc ctgtgcctgt tcacgcagcg tttttccctg ccattcacca gcggttgccg
      781 tttcagcaaa ggtttttgcg ccgccgccaa gcgtaacgtc ggcacgagcg ttaagcagct
      841 gttcggtaat cgatcctttt ccgccttttt ccagagcgtt acccggacat ttttcactgg
      901 tcgcgctcgg accgtagcat ttgcgcgaag tcacatgtgc caccagcgca gcgggcgtgg
      961 catcctgcaa ctctgcggta gaaacgttac cggtcgccag acctgcggct tttgccattt
     1021 ccagaatcgt tgggtgatct ttttcgtgaa tatcgacgcc cagcgcgccg ttataggttt
     1081 tgacatcggt tgaccaggcg gttgctgatg cagccgagtc ggtgacgtag tccggtttgc
     1141 cagttttttt attcagcgca tagtgagtgt attgcccggt aagcggtaag gcatcgatac
     1201 ctttaaaaaa gccgcccgca ccttcggcat aattacgtgc ggcagtaatt tccgaatccc
     1261 ccatcccatc gccaatcagc aaaataatat tttttgcagg tttatcgcta agagaatcac
     1321 gcagagcggc ggtctgatca cccgttaagc ggcgagcacc gccgggtgca gtaatatcgc
     1381 cctgagcagc ccggttttcc agaacaggca tttctggtgt ccgggctttt gtcacagggg
     1441 taaacagtaa cggtaagagt gccagtgcaa tagtgctttg tttcacttta ttttctccat
     1501 gtacaaatac attaaaaaat aaaaacaaag cgactataag tctcggccgt gacaacttta
     1561 tgacagctgt tgaaaagatt aactttttac tgacaaggat gcttcaataa cttctttacg
     1621 taatcgcgca gcagctctgt atcgtcgtca ggaatgctgg catcgggctt tacctcgtac
     1681 agcgccccct ctacctgatc aatcaaccgc tgttggtcat tttgcgccat attgcgaagc
     1741 attgcagtga cgataatctc caaagcttct acctgcgcac acagttcttt cgactcttct
     1801 tctttttggg caagcttaaa taacaactca gcaatgagat ttttcatgtc tgtatttcct
     1861 tatccaaagt atggagaagt taacattatg tttcacaact tcatagtgtc tggaagtatt
     1921 atttttatga caaaggaaat actttgcgca aatatttttt gtttgattgc cagcgaaacg
     1981 tttctcttat ttaaataatt acccacacaa aatataggct tttaattatc aacctttatt
     2041 ataaataaaa gctatgaaat atattctctt cgttaactta taattaagag aaaaaaactc
     2101 accgcattgc catttaagcc aggaataaat tagcttcccg ttcatcattt ttgtgatctt
     2161 gcgcacatct ttgagataac gcactttctt caccagtacg ttgcacgctt tgtcagcaga
     2221 agattgcaac aatctttcag aaggccgcga atcccccgtt ttgtcgtggt aatgtatgca
     2281 ctttgtgtta gacgggatag gtttttaaga tggaaaaact gcgggtagga atcgtttttg
     2341 gtggtaaatc agcggaacat gaagtgtctc tgcaatcggc aaaaaacatt gtcgatgcca
     2401 ttgataaaag tcgcttcgac gttgtgctgc tgggcattga taaacaaggg caatggcacg
     2461 tcagcgatgc cagcaattat ctgctaaatg cagacgatcc tgcccatatt gcgttgcgcc
     2521 cttcggcgac cagccttgcg caggtgccag gtaaacatga gcatcagctt atcgacgcgc
     2581 aaaacggtca gccgttgccg acggtggatg tcattttccc gattgtccac ggtacgctgg
     2641 gcgaagatgg ttccttgcag ggaatgctgc gggtcgccaa tttaccgttt gtaggttctg
     2701 atgttctggc ttcagcagcc tgtatggata aagatgtcac caaacgtctg ctacgcgatg
     2761 ccgggctgaa cattgcgcca tttattaccc tgacgcgcgc taatcgtcac aacatcagtt
     2821 ttgccgaagt ggagtctaaa ctggggttac cactgtttgt aaaaccggct aatcagggct
     2881 cttctgttgg tgtcagcaaa gtaaccagtg aagaacagta cgcaattgcc gtcgatctgg
     2941 cgttcgagtt cgatcataaa gtgatcgttg agcaagggat caaaggtcgt gagatcgaat
     3001 gcgcagttct gggcaacgac aatccgcaag ccagcacctg tggcgagatc gtactcacca
     3061 gcgatttcta tgcctacgac accaagtaca ttgacgaaga tggcgcgaaa gtggtagttc
     3121 cggcagccat tgcaccagag atcaacgata agatccgggc gattgccgtt caggcttatc
     3181 agacgttggg atgcgcaggc atggcgcgtg tagacgtgtt tttaacccca gagaacgaag
     3241 tggtgatcaa cgagatcaac acactgcctg gcttcaccaa catcagtatg tatccgaagc
     3301 tgtggcaagc cagcggtctg ggttacaccg atctgatcac acgtttgatt gagctggcgc
     3361 tggagcgtca cgctgcggat aacgcattga aaaccacaat gtaatatttt aacgcccgat
     3421 aatattctta ttgggcgttt tcagtttctt catcgcgacg acgaataatc aaccccgcca
     3481 gccagaaact aatcacccac gtcaccagac cgacggcata ggtctgccaa ccttttgctt
     3541 caaatcccag tacccccacc acgccattaa gaatgaatat aaggccaatg gcgaacgcat
     3601 agtagtgcca gtcacggcgg atttttacag gcagattcat ggcagctccg ttaacgaaaa
     3661 acaacatcct tgcatattct gtaacaaccg tctgtggtgt agacaacatt ttcgtaatta
     3721 ttaataaatt taacttaagg ttgccatttt gtgactcaca acgaagattc acgcttcggg
     3781 aatattccca acatcaattt accatgattc tgatattgac aatctaaatg acctgtcaga
     3841 cttgccataa gccgctggta tcacaccaga agagaagcag atgacttccg gaggttttta
     3901 tggctgattt caccctgtca aaatcgctgt ttagcggaaa atatcgcaat gcctcttcaa
     3961 cgcctggcaa cattgcctat gcgttgtttg tgctgttttg cttttgggct ggggcgcaat
     4021 tgctgaacct gttagtgcat gcgcccggcg tctatgagcg tttaatgcag gtccaggaaa
     4081 caggtcgccc acgggtggaa attggtttag gtgtcggcac cattttcggg ctgatcccgt
     4141 ttttagcagg ctgcctcatt tttgcagtgg tggcgctatg gctgcactgg cgacatcgcc
     4201 gccagtaaaa tttacgttgc cagatgcggc ctgaaacgcc ttatctggcc tgacattcct
     4261 gtttgatcac tgtttcatac aacgcgcccg ccgttcgtct acgcgcttcg caaaccaggc
     4321 cgtagtcagg ttgcgggtga ttttcgggct ttccagttga atgccaggca acatttcgcg
     4381 gggtagtgat ttacccgttt tcgcttcggc aagttgataa actttcttgt acagcgccgt
     4441 ctcctcaaaa gagaagctgt cacctttctc taactgacgg cgaatttcgc tgtcgctcat
     4501 ccccagtttt cccgccagtt tgcgcgttgc cagttcggtt ttcccgggtt ctttactgtc
     4561 gtaacgaatt aaatcaccgt ccagtgccag cttcacgccg ctggccttac tgactgcgtt
     4621 ctgaaacgcg gcattacggc tggcgtacca gccagcgtta aaatcagcaa agcggtatat
     4681 cggtgcgcta tagctggcgg gataattcag taaatggtaa gtaccaaacc acaacccgcc
     4741 gcggcgactg aagacttcct gacgaactgt accgtccatt ttccacggat accctttggt
     4801 atgctgttcg gcaaaagcaa tgctgacctg catcggccca ccggtgcgca ccggattaag
     4861 cgaaccaaac agcgtctgcc ccattggtac catgctgatc agatcgtcaa aaatcgcgct
     4921 caattgcttt tctgtacgca ccgaatctaa ccgttcgcta taacttttgc cgtttggcga
     4981 tttgattttt agcgcggtat gaaccagaaa agcaggaatg tgcatccgtt cagcacgacg
     5041 gtcaatctct tgccaggcga tcttgcttaa gcccggaacg gccggatctg cctgataatt
     5101 tgactcctgc tgcgccaccg ccagcaccga gtagacgttt tccagcgtcg gtgccagccc
     5161 ctgactttca aaggtggtgg cgagatcttt cgcccaggca tcgcggtctt tcacgctcgc
     5221 aggcatcttc tgccgcacga cgctcgctac atctatcgct ttttcgccct ttttcaacgg
     5281 ttgcggagcc tgactactac accccgctaa aaccagaacc gccaacaatg acagcgaaga
     5341 tagatgattg acgcgcgaca ttacttctcc tttttagctc aaggtatggg ttacttcctg
     5401 aattttgtca ccatccagct catgttcaaa gctgcgcaga cgtttgtaga tagacatcaa
     5461 ctcaaccagt gtggtccatg agttaatcag gtactggaaa gcaccgcgaa cctgaccaaa
     5521 aacgttggta atctgcgtca tcaggccgag cgtaatcgta ccggcaacaa ttgacggaaa
     5581 cagcaagaac aaaccgaaaa cgttatcgac ctgcaaatag agaatgcggg cgatgttgaa
     5641 atacatatag tgaaaataga ggcgaaaata gtttttccgt acggcgctaa acagctcgcg
     5701 taccgtaggc ggcgtcgcgc gcgtggcatc gtcttcacca taaaccagct ctttacggta
     5761 ggcggcctct actcgctggt ttttaaactc cagccccggc agtttgatcc ctaccactgc
     5821 cagcaatccg gtccccatca gcgaccagac gatagcggca atcaccagac catacggaat
     5881 gtgcccgata atcggcagct ccggcacatg cgcggagagc gttaccagca ccggcaggaa
     5941 ggcgatcaac gtcatgatgg cgttgataaa actgacgccc atattctcca gcgttgaagc
     6001 aaaacgcatg gtgtcttcct gcacacgctg tgcggcacct tcgatatgac gcagttgttg
     6061 ccagttcgcc atgtaatatt cgttcatcgc cgtacgccag cggaacacgt agtgactgac
     6121 aaagaagttg ttcagtacac tgatcaccac cgcgatcagc gcaatcccca gaaagacgcc
     6181 cacttcgcgg taaaattgtt cgatagtgac tttatgcggc gaacttagcg cggtttgaat
     6241 cagatcatag aacggcgcat accaggcgtt gacggcgacc ccgacttcca ccaaaaacca
     6301 agtgacgaag ataatcagcg cagtaccgag tattgaccag tattgccaac ggtgcgggct
     6361 gtagataaac cagaaaaatg caaaaagtcc tacgcaaaca atgtagtaag cgtaaaaaat
     6421 caggaaatcc aacgaccaga aacgcgcggc gctaatcggg atctgcccgg aagcgccggt
     6481 gatacgtgcc acccagtcac ccccaccggc ttgccagaag ataacggcaa tcaatgccca
     6541 aacaaaggcc gagagaaaaa acgttcccgg ctttgggaaa aaagacttaa acatatgcac
     6601 tcctgctaac ttcttatcgt tttaacaact gctgtgtatt aaccgcatga ccgctacaaa
     6661 gcgtcagcca aatgaaaccg cccattgtga cccatagcaa ggcgcttagt tcgccagcct
     6721 gaccataaga attgtttcga cggatttcac aaaccaaaga ttgtttacat aagcggatta
     6781 acacgcgcag gatcacaaat gcaggcattg gtgagcagga aaaagatcgt gtttacgcgc
     6841 cgttacgttt ttaacactat tattgcacta attcgccctt tgcaatccat caatgagtag
     6901 tataaatacg ctcagttacc ttcattcaat ctatggacac caccgttgaa acgtagtctg
     6961 cttttttctg ccgtgctgtg tgcggcgtca ttgacctccg tccatgcggc gcagccgatc
     7021 actgaaccgg agtttgcctc tgatattgtc gatcgttatg ccgatcatat tttttacggc
     7081 agcggtgcca cggggatggc gctggttgtt atcgacggta accagcgcgt ctttcgtagt
     7141 tatggtgaaa cgcgacctgg taataacgtt cgcccgcagc tggattcggt cgtgcgtatt
     7201 gcttccctca ccaaactaat gaccagtgaa atgctggtga aattgctcga ccaggggacc
     7261 gtaaagttaa acgatccgct aagtaaatat gctccgccag gcgcacgcgt gccgacctac
     7321 aatgggacgc ccattaccct agtgaatctg gcaacccata ccagcgccct gccccgtgaa
     7381 cagcccggtg gcgcggcaca tcgtccggtg tttgtctggc caacgcgcga gcaacgctgg
     7441 aaataccttt ctacggcgaa gctgaaagcc gcgccaggca gtcaggcggc gtactctaac
     7501 cttgcgttcg acctgctggc agatgcactg gcgaatgcgt ccggcaagcc ttatacccag
     7561 ttgtttgaag agcagattac ccatccattg gggatgaaag acaccaccta caccccctca
     7621 ccggatcagt gccgccgttt aatggttgct gagcgcggtg ccagtccgtg caataacacg
     7681 ctggcggcaa ttggcagcgg cggcgtctat tccacgcctg gcgatatgat gcgctggatg
     7741 cagcagtatt tgtcatctga tttttatcag cgcagtaacc aggcagatcg catgcagacg
     7801 ttgatttatc agcgcgcgca gtttacgaaa gtgattggca tggatgtccc cggcaaagcg
     7861 gatgcgcttg gtttaggctg ggtatatatg gccccgaaag agggtcgtcc gaggattatt
     7921 cagaagacag gcggtggtgg cggtttcatt acctatatgg cgatgatccc gcagaagaat
     7981 attggcgcgt ttgtggtggt cactcgttcg ccgttgacgc gctttaaaaa tatgagcgat
     8041 ggcatcaatg atctggtgac cgagctaagc gggaataaac cgttggttat ccccgcatcc
     8101 tgattaatac tctgaaggca aacggttgat agcgaccagt ttgcctttat tcatttcgat
     8161 atagccgcct ttacgcaaag cagcgagaac ttctgcgacg acagaacgcg atatgcgtgt
     8221 acgttgatgg atataattca tcacaccaat acgtgatcgc aattcttcat tccagtcaat
     8281 cattgataat aatgttgcgc gggtttgttt ggctggtaga tgatatcccg tacaatttcc
     8341 ttctgatatt aatttgtatg gtatatcgtt tttcattaaa ccatcagcca gccccataat
     8401 ataaggagcc tgggtaatac cgataagtac gttttcttct ctacgcagag agataactcc
     8461 ttctaaaata acgaaagtat cttcgttatt gacatcactg gaaaatataa cttgcttttc
     8521 attattaaac tcgaagcgcg taccgtatct ggacaaacat ttatcgagct taccaaattc
     8581 ctgaagaggt ttaactacag ataacatttg cgcgtccttt gcagtaatgc ccgtcaaatc
     8641 cttgacgggc attatttaga ttaaattacc agtatttctt cggagtgaag aatattacca
     8701 agtatattta acacccacgt tcgcggacca gtcttgatct acgtcaccac caccgaggta
     8761 gttagcatcg gtataggcgc tgaagttctt ggtgaagctg aactgagtac ccagaccaac
     8821 acgtaccgca gacccttcag taccgttat
//
LOCUS       DAJTUP010000137         8841 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTUP010000137 DAJTUP010000000
VERSION     DAJTUP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.137"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..894)
                     /locus_tag="OO468_003539"
     CDS             complement(<1..894)
                     /locus_tag="OO468_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR6260513.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            954..1232
                     /locus_tag="OO468_003540"
     CDS             954..1232
                     /locus_tag="OO468_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260514.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO468_003541"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO468_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR6260515.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO468_003542"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO468_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR6260516.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="OO468_003543"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="OO468_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR6260517.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="OO468_003544"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="OO468_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR6260518.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="OO468_003545"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="OO468_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="OO468_003546"
     CDS             complement(4912..5442)
                     /locus_tag="OO468_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6260519.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO468_003547"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO468_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR6260520.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="OO468_003548"
     CDS             complement(6411..7634)
                     /locus_tag="OO468_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260521.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8215..>8841)
                     /locus_tag="OO468_003549"
     CDS             complement(8215..>8841)
                     /locus_tag="OO468_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6260522.1"
                     /translation="AGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTP
                     IKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGS
                     FIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALM
                     ALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2572 a         1737 c         1848 g         2684 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg ttgttcatat
     6841 ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt tttattatcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt gtgtataaaa
     7801 aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa cgctcatttt
     7861 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
     7921 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
     7981 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
     8041 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
     8101 ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta tgattttcta
     8161 cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat ctatttaaaa
     8221 tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg tcaggaatgc
     8281 agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca ggcttgcgcc
     8341 ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg tcatcagaac
     8401 agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga caacggcgct
     8461 taatgggatg gcgaagggat tatctgctcc tgcatgagcg gcaatccacg ctgagggaat
     8521 aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca gtacatcttt
     8581 aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta ctacagcttt
     8641 ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac tacaacaact
     8701 cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac aacaatttgc
     8761 tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca ggataatact
     8821 ggcaagaacg gagacgccgg c
//
LOCUS       DAJTUP010000138         8792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTUP010000138 DAJTUP010000000
VERSION     DAJTUP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.139"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(24..506)
                     /gene="ybiA"
                     /locus_tag="OO468_003550"
     CDS             complement(24..506)
                     /gene="ybiA"
                     /locus_tag="OO468_003550"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR6260523.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            626..2776
                     /gene="dinG"
                     /locus_tag="OO468_003551"
     CDS             626..2776
                     /gene="dinG"
                     /locus_tag="OO468_003551"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR6260524.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2804..3766
                     /gene="ybiB"
                     /locus_tag="OO468_003552"
     CDS             2804..3766
                     /gene="ybiB"
                     /locus_tag="OO468_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR6260525.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            3907..4992
                     /gene="hcxB"
                     /locus_tag="OO468_003553"
     CDS             3907..4992
                     /gene="hcxB"
                     /locus_tag="OO468_003553"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR6260526.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5221..5481)
                     /locus_tag="OO468_003554"
     CDS             complement(5221..5481)
                     /locus_tag="OO468_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR6260527.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASPLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(5746..6012)
                     /gene="ybiI"
                     /locus_tag="OO468_003555"
     CDS             complement(5746..6012)
                     /gene="ybiI"
                     /locus_tag="OO468_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR6260528.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(6086..6763)
                     /gene="ybiX"
                     /locus_tag="OO468_003556"
     CDS             complement(6086..6763)
                     /gene="ybiX"
                     /locus_tag="OO468_003556"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR6260529.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(6805..8792)
                     /locus_tag="OO468_003557"
                     /pseudo
     CDS             complement(6805..8792)
                     /locus_tag="OO468_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         2029 a         2209 c         2428 g         2126 t
ORIGIN      
        1 accgcccata agccaccgag acgttacttc tctatagcca attgttcgcg caactccatt
       61 aaaaggtagc ccagtcgatt cttgccctta ccattaccac cgtctcccca gtaagcatcg
      121 ttttccgtat gctcaaccag ttttgcgggc gcggttgcca gcaagagcac acgcagttct
      181 gcatgctgtt cgaatttagc gcgaagtgct ttacgcatca cctgttcttt aaccgactcc
      241 cagtttttac gaagtggctt ggaacgatta cgccccatgc gagcggcgac catgggggaa
      301 gaaacccgac ggatctcttc gcggtatttt tcatcaagga atttttgtgc ctgaaaatag
      361 tgttcggagg tgggccaggt attcccgtca accttgatgg gcctggcggc aaagttagaa
      421 aaatcaccgt agtcatcgct ggtgctgtag aaatttatga tggtgtcttg catgacgtgt
      481 tggatccttt gtgctcgaac gggcattaaa ccgcattatg ttggtggtta ttgcgagccg
      541 ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattggctg
      601 tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc
      661 cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg
      721 gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat
      781 tgaagccccc accggcgttg ggaaaacgct ctcctatttg atccccggca tcgccattgc
      841 ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcgttgc aggatcagat
      901 ttacagcaaa gatttaccgc tgctgaaaaa gatcattccc gatcttaaat tcactgccgc
      961 tttcgggcgt gggcgctacg tttgtccacg taatctgacg gcgctcgcca gcactgaacc
     1021 cacgcaacag gatctgctgg cgtttcttga cgacgaactg acgccaaata atcaggaaga
     1081 gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg atggtctgcg
     1141 cgatcatacg gatatcgcta tagatgatga tctctggcgt cgtttgagta ccgacaaagc
     1201 cagctgcctc aaccgtaact gttactacta tcgtgaatgc ccgttttttg ttactcgtcg
     1261 ggagattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga
     1321 aagcgaagcc gtactgcctg acccgaaaaa tctgctgctg gtgctggacg aaggtcatca
     1381 cctgccagat gtggcccggg atgcgctgga gatgagcgct gaaatcaccg tgccctggta
     1441 tcgactacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc agtttcgccc
     1501 gaagaccatc ccaccgctgg cgatccctga acgtttgaat gcgcattgtg aagagttgta
     1561 tgagcttatc gcctcgttaa acaacattct caatctctac atgcctgccg ggcaggaggc
     1621 agagcaccgt tttgcgatgg gcgaactgcc tgatgaattg ctggagatct gccagcggct
     1681 ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga
     1741 gaaaaccggc agccacgata ttgtgcgtct gcatcggttg attttacaga tgaaccgcgc
     1801 gctggggatg tttgaggtgc aaagcaaact ctggcggctg gcgtctctgg cgcaatcttc
     1861 cggtgcgccg gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc tacacctctg
     1921 gtttcactgc gtgggaatac gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat
     1981 tccgcacatt attgtcacct ccgcaacctt gcgttcgctg aatagttttt cgcgtttgca
     2041 ggagatgagt ggtctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt
     2101 taaccactgc gaacagggca aaattgttat tccccggatg cgctttgagc cttccatcga
     2161 caacgaagag caacacattg ccgaaatggc ggcctttttc cgtgaacagg tggagagcaa
     2221 aaaatatctc ggtatgttgg tactgtttgc cagcggacgg gcgatgcagc gctttctcga
     2281 ctatgtgacg gatttacgcc tgatgttgct ggtgcagggc gatcagccgc gttatcgctt
     2341 agttgaactg caccgcaaac gcgttgccaa cggtgagcgt agcgtgctgg tgggcttaca
     2401 gtcatttgcc gaagggcttg atttgaaagg tgatctgctc agccaggtgc atatccacaa
     2461 aatcgctttt ccgcccatcg acagcccggt ggtgatcacc gaaggggaat ggctgaaaag
     2521 cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc gcctcgttta acctgattca
     2581 gcaggttggg cgactgattc gaagccacgg ttgctggggc gaagtggtta tctacgacaa
     2641 acgcttgctg acaaaaaatt atggcaagcg actactggat gcattaccgg tatttccgat
     2701 agagcaaccg gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg
     2761 ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat
     2821 cattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg
     2881 cggtctgtat gctcatatgc tcaatggtga agtccctgac cttgagttgg gcggcgtatt
     2941 gattgcgctg cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat
     3001 gcaaaatcac accatcaagc tgacgccgcc tgcaggcaag ccgatgccga ttgtcatccc
     3061 cagttacaac ggcgcgcgta aacaggctaa cctgacgcca ttattggcga ttctgctgca
     3121 taaactcggt tttcctgtgg tggttcacgg agttagcgaa gatccaactc gcgtgctgac
     3181 agaaaccatt ttcgaactga tgggtattac accaacgttg cacggcgggc aggcgcaggc
     3241 gaagctcgac gaacatcaac cggtgtttat gcctgtcggc gcgttttgcc cgccgctgga
     3301 aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa
     3361 actggcgacg ccatttgccg aaggtgaggc actgtgtctt tccagcgttt cgcatccgga
     3421 atacattgga cgcgtcgcga agttctttag cgatatcggc gggtgggcgt tactaatgca
     3481 tggtactgaa ggcgaagtgt atgctaatcc gcagcgctgc ccgcagatca atctcattga
     3541 ccgtgaaggg atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg agttactgcc
     3601 acaagcaaaa gatccggaaa ccacggcgca gtggactgag cgttgcctgg ctggcagcga
     3661 agcgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggtgaagc
     3721 ggcaactatc agcgatggcc tggcgcgcgt taatcaggca ttttaatttt tatctcagcc
     3781 ggatgagcca cgtcatccgg cttttacccc accgcaatat gaaattcctg catctttatt
     3841 gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag
     3901 ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagttt tattcaggct
     3961 gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagttgccga tcatttaatc
     4021 gcggcaaacc tggcagggca tgattctcat ggtattggca tgatcccaag ctatgtgcgt
     4081 tcctggagtc aggggcatct gcaaattaac catcatgcca aaatcgttaa agaggcgggg
     4141 gcggcagtca cgctcgatgg cgatcgcgca ttcggtcagg tcgcggcaca cgaagcgatg
     4201 gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct acataactcg
     4261 catcatatcg gccgtatcgg ttactgggcg gagcagtgtt cagcggcggg gtttgtctct
     4321 atccactttg ttagcgtggt cggtattcca atggtcgcgc cgttccacgg tcgcgacagc
     4381 cgctttggca ccaatccctt ctgtgtggtt ttccctcgta aagataattt cccgctgttg
     4441 cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc
     4501 gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg
     4561 gtaatgcagg agtcgccgtt ggggtcgctg ttgacctttg ccgaacataa aggctacgcc
     4621 cttgcagcga tgtgtgaaat tcttggcggg gcgctttctg gcggtaaaac gacgcatcag
     4681 gaaacgttgc aaaccagccc cgatgccatt cttaactgca tgaccactat catcatcaac
     4741 ccggaactgt tcggcgcgcc ggattgtaac gcgcagaccg aagcctttgc cgagtgggtg
     4801 aaagcctcgc cgcatgatga cgataagccg attttgctac cgggcgagtg ggaagtgaac
     4861 acgcgtcgcg aacggcagga gcaggggatt ccattggatg cgggaagctg gcaggccatt
     4921 tgtgatgcgg cgcggcagat tggtatgtcg gaagagacgt tgcaggcttt ctgtcagcag
     4981 ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat
     5041 caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga
     5101 cgtagcgtaa acgcctgatt cgtccaacgg ttcggatttt gtaggcctga taagacgcgg
     5161 tagcgtcgca tcaggcatct gttgccggat gcggcataaa cgccttatcc gtcctacgaa
     5221 ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg aagtgatgct
     5281 gtatccactg gcaccggctg ccgccgcttt ctcagccagt ttcgcttcca gggcatcgag
     5341 gggggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc
     5401 cgttaccggt tccgccgcga acacgccaaa tgacagagtt gaaagagcca tagcagcaac
     5461 aacagtattg atagttttca taattaatct ctcgcaggtg atttttgtat aagtgacgtt
     5521 gtttcgtcga tgtgatgagt atcacgtttt tttacgagag ataaaatcga agagaattga
     5581 ccgccttgtt caaataaatt gattgatatc taacatactg ataaaccatt tatttgttgt
     5641 acttatggac ttgttttatg ccgcgtggca gtcacagcag cgttaaaggc acggaatgcc
     5701 acgcaaaata caaattatac tcgctatgca tctcgccagt tgtagttaac gtaactgtct
     5761 gtctttcgaa cctctgcgat tatatcctgt atacgctggt ttttgtaaat ctttctcctg
     5821 ctgacaatga atacataagc gcacgccagg aatggcttcc cgacgggctt gcgggatggg
     5881 ggcaccgcac tcttcacatt catacaggct ttcgccacgc ggaatttcac cccgagcgcg
     5941 ggcaatcgca tcttcaattg tactgttgat ctgttcgttg acggcgtcat cgttagccca
     6001 agcggatgcc atatcgtcct ccccatatca atgcttgtac agttaagtgt agctaatata
     6061 gggacgaacc tgggcagttc aagcgtcaga tctccgacca ttcccgcagc agattatgat
     6121 aaagattgag cagcgacagg atctcttcat tttcaccata gcggcttttc agcgactgaa
     6181 tatttttgtc cagttcaaac agcatggcgc gctttttatc atcgcggatc atcgactgga
     6241 tccacataaa tgatgccact cgtacgccgc gggttacggg tgtcacgcaa tgcaggctgc
     6301 tggagggata caacaccaga tcacctgccg ggagttttac ccgatgttgt ccaaaggtgt
     6361 cattaacgac cagttcgccg ccgtcgtagc tttgtggatc gcttaaaaac agcgtggcag
     6421 aaaggtcagt gcgcatccag ccgttttgtg gatggctgcg tactgcgcca tccacatgga
     6481 aaccataggt ttcattgttc tgatagcgat taaacagcgg cgtggaaagg gtacgcggca
     6541 aggcagcggc aaagaacaat gcatgttggt taaccgcgtt cagcacctca ttttgcagtg
     6601 cggcgtataa cgtgctgcgg gtgtctactt gctgattgtt cttaacttgt gcaccttgtg
     6661 ctccggtggt gacgcgtcca tccacccatt cggcttgttc cagttgttcg cgaaaatggg
     6721 cgacgtcctg tggcgataac acgccgggaa tgtggtacat catacaagtt ctccaaaaag
     6781 tggggcctgc gccccacatc tgaatcagaa atgcatattg gctgtgagca agaaggttct
     6841 tggctcgccc gggtgataac ggtagccgct cttgttgatt gaggcgacgt aatcggtatc
     6901 aaacaggttg taaacgttta gctggaagtc gagattgcga ttaacccgat accccagttt
     6961 ggcatcggcg acccagtaac cttctgtaaa tgctggcgtt cccaccgcgc cgtctgaacc
     7021 tttatgtata ctgccgatat agcgtgcgcc cgcgccaaca gagatatcat cggttgcctg
     7081 atattggctc cataaggtga aggcgtgctc cggggtatac ggcagcgatg aggaaccatc
     7141 ctgggcaaca tctttgccgt ttttgatggt tgctttttgc tgggtatagc cgccaatcac
     7201 ctgccacgcg ggagtgatat tccctgccac ggatatctca tagccttcga cgcgtttctt
     7261 accgtattgc gagtaagtcc cgtcatcatt ttgctcaact tcattttcga tatcagtacg
     7321 gaacagcgcg gcggtgagca acaggcgttt atccagaacc tgccatttgg tgccaatctc
     7381 gctggtgttg gctttttgcg gtttaaaatc ggtgcggttg gcactgttac cactgccaga
     7441 ctgcgcaagg gcgaagttgt tgccgcccgg aggctgctgg gaaacggcat agttaatata
     7501 gacattgccg ttttccgtca ggtggtacag cgccccggct ttccagttca ccagattgcc
     7561 cgacttggcg gtgtcgacgg tggtgactgg agaacctttt gccacaccag ctgggcaggt
     7621 gatggcaccg cgtccgctgc cgccgcaggc ggtggcactg tcatattcag tatgataatt
     7681 atccagacgg atcccgccgt tcagctcaaa atcacgggtg atttgcagcg tatcgaaggc
     7741 gtaaattgcg aaggtatccg tctgaccatt ggcgtttgcg ccgttgcgcg tcaggccgcc
     7801 gggatgaatt ctgctgtcag gatgataaat atttaccgcc ggtaacgtca ccggattaac
     7861 gccatagtta gtctgcgttt cacgggtaaa ttccacgccg gtactgacat catgaccgat
     7921 agaaccggta tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt tattactcac
     7981 atctttggta ttcgccgtgc gtgactaggt ccagctattc acatcgctgg tgggctgagt
     8041 aatattcgac gccccgccca taatcgccgt catcaggtaa tcctgcttta cgcgcgacca
     8101 acgggtagta ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac gcatggtggc
     8161 ggtgtcggtg gtcgaatcgt cgtaatcgga atccgtgccg taaaagttat gagtatcaac
     8221 ttttccggaa tgattcaggg ccgccgctcc cgcagatggg gcagaatagc ccggcaaacc
     8281 gatggtcgga atgccgccgt ctggcgtgtt gtgctgagtg acatgcagat aattaagata
     8341 caaacgattt gctgtaccaa ggccaaaagc gacagaaggg gcgacgccgt aacgctcatt
     8401 tttgactttg tcgcgtccgg catcgtgggt tttttcgccc atcacattca ggcgcaccgc
     8461 ggtagtatca ccaatgacct gattgacgtc cagcgtgccg cggcggaacc aggcgctgcc
     8521 aatactggcg gaggcgtcaa tgccggaatc attgcgcggc tgcttgctga tcatattgat
     8581 cgagcctgtc ggtgcgctgc gcccgtagtc ggtgccggac ggccctttaa tcacttcgac
     8641 ctgctcggta ttgaaggtgt cgcgcgagac gctgccgata tcgcgaatac catcaatata
     8701 aatactgtta gaggtatcgg caccacgcat ataaatggcg tcgccagtgg tggagttacc
     8761 gttctcaccc gcaaaaaacg cacccacgcc gg
//
LOCUS       DAJTUP010000139         8742 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTUP010000139 DAJTUP010000000
VERSION     DAJTUP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8742
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.141"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            157..1275
                     /locus_tag="OO468_003558"
     CDS             157..1275
                     /locus_tag="OO468_003558"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR6260530.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1341..1589
                     /locus_tag="OO468_003559"
     CDS             1341..1589
                     /locus_tag="OO468_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR6260531.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1654..2022
                     /locus_tag="OO468_003560"
     CDS             1654..2022
                     /locus_tag="OO468_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR6260532.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2116..2769
                     /gene="nfsB"
                     /locus_tag="OO468_003561"
     CDS             2116..2769
                     /gene="nfsB"
                     /locus_tag="OO468_003561"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR6260533.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            2877..4124
                     /gene="ybdG"
                     /locus_tag="OO468_003562"
     CDS             2877..4124
                     /gene="ybdG"
                     /locus_tag="OO468_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR6260534.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4192..5568)
                     /gene="pheP"
                     /locus_tag="OO468_003563"
     CDS             complement(4192..5568)
                     /gene="pheP"
                     /locus_tag="OO468_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR6260535.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5669..>8742)
                     /gene="cusA"
                     /locus_tag="OO468_003564"
     CDS             complement(5669..>8742)
                     /gene="cusA"
                     /locus_tag="OO468_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR6260536.1"
                     /translation="IWGTWTIINTPVDALPDLSDVQVIIKTSYPGQAPQIVENQVTYP
                     LTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTDPYWARSRVLEYLNQVQGKLPAGVS
                     AELGPDATGVGWIYEYALVDRSGKHDLADLRSLQDWFLKYELKTIPDVAEVASVGGVV
                     KEYQVVIDPQRLAQYGISLAEVKSALDASNQEAGGSSIELAEAEYMVRASGYLQTLDD
                     FNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIAELNGEGEVAGGVVILRSGKNAREV
                     IAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRAIDNLSGKLLEEFIVVAVVCAPFLW
                     HVRSALVAIISLPLGLCIAFIVMPFQGLNANIMSLGGIAIAVGAMVDAAIVMIENAHK
                     QLEEWQHQHPDATLDNKTRWQVITDASVEVGPALFISLLIITLSFIPIFTLEGQEGRL
                     FGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGKIPPESSNPLNRFLIRVYHPLLLKV
                     LHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQINEGDLLYMPSTLPGISAAEAASMLQ
                     KTDKLIMSVPEVARVFGKTGKAETATDSAPLEMVETTIQLKPQDQWRPGMTMDKIIEE
                     LDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIGIKVSGTVLADIDAMAEQIEEVART
                     VPGVASALAERLEGGRYINVEINREKAARYGMTVADVQLFVTSAVGGAMVGETVEGIA
                     RYPINLRYPQSWRDSPQALRQLPILTPMKQQITLADVADVKVSTGPSMLKTENARPTS
                     WIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSVAFSGQFELLERANHKLKLMVPMTL
                     MIIFVLLYLAFRRVGEALLIISSVPFALVGGIWLLWWMGFHLSVATGTGFIALAGVAA
                     EFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEALYHGAVLRVRPKAMTVAVIIAGLL
                     PILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFIIPAAYKLMWLHRHRVRN"
BASE COUNT         2143 a         2414 c         2129 g         2056 t
ORIGIN      
        1 tctgccataa cttgccacat tgaaaaagta gccaaagtgc ttcaggcacc atcagttcac
       61 catcaccaaa aaaaagaaac attccagaac ttccgcatct cgcccagccg ctccgacttc
      121 tatactgaat agaaaacgtc aacttaagag aaacctatgc cattacccga ttttcatgtt
      181 tctgaacctt ttaccctcgg tattgaactg gaaatgcagg tggttaatcc gccgggctat
      241 gacttaagcc aggactcttc aatgctgatt gacgcggtta aaaataagat cacggccgga
      301 gaggtaaagc acgatatcac cgaaagtatg ctggagctgg cgacggatgt ttgccgtgat
      361 atcaaccagg ctgccgggca gttttcagcg atgcagaaag tcgtattgca ggcagccgca
      421 gaccatcatc tggaaatttg cggcggtggc acgcccccgt ttcagaaatg gcagcgtcag
      481 gaggtatgcg acaacgaacg ctatcaacga acgctggaaa actttggcta tctcatccag
      541 caggcgaccg tttttggtca gcatgtccat gttggctgtg ccagtggcga tgacgccatt
      601 tatttgctgc acggcttgtc acggtttgtg ccgcacttta ttgccctttc cgccgcgtcg
      661 ccatatatgc agggaacgga tacgcgtttt gccccctcac gaccgaatat tttttccgcc
      721 tttcctgata atggcccgat gccgtgggtc agtaactggc aacaatttga agccctgttt
      781 cgctgtctga gttacaccac gatgatcgac agcattaaag atctgcactg ggatattcgc
      841 cccagtcctc attttggcac ggtggaggtt cgggtgatgg ataccccgtt aacccttagc
      901 cacgcggtaa atatggcggg attaattcag gccaccgccc actggttact gacggaacgc
      961 ccgttcaaac atcaggagaa agattacctg ctgtataaat tcaaccgttt ccaggcctgt
     1021 cgctatggtc tggaaggcgt cattaccgat ccgcacactg gcgatcgtcg accgctaacg
     1081 gaagatacct tgcgattgct ggaaaaaatc gccccttcag cacataaaat tggcgcatcg
     1141 agcgcaattg aagcactgca tcgccaggtc gtcagcggtc tgaatgaagc gcagctgatg
     1201 cgcgatttcg tcgccgatgg cggctcgctg attgggctgg tgaaaaagca ttgtgagatc
     1261 tgggccggtg actaagccgg gaattgcctt acagaagaat atccctgaca atggtgtttt
     1321 aacttacatt taacaatgtt atgaaacacc ctttagaaac cttgaccacc gcagcaggca
     1381 ttttgctgat ggctttcctc tcttgcctgc tgctgcccgc ccccgcactg gggctgacgc
     1441 tggcacaaaa actggtgacc atgttccatc tgatggatct tagccagctt tacactttat
     1501 tgttttgtct gtggttttta gtgctgggcg ctattgagta ttttgttctg cgctttatct
     1561 ggcgacgctg gttctcgctg gcggattaaa cgtcgattat cgccgaactt cgcggcatac
     1621 tttgcttatt ctccttcgcc taaaggaacg tttatggata agcaatcact gcacgaaacg
     1681 gcgaaacgcc tagctcttga gttacccttt gtcgagcttt gctggccttt tggcccggag
     1741 ttcgatgttt ttaaaattgg cggcaagatt tttatgctgt cgtcggagct acgcggcgtc
     1801 ccctttatca atctgaagtc cgatccacaa aaatccctgt taaatcagca aatataccca
     1861 agcattaagc cagggtatca catgaataaa aagcactgga tttcggtgta ttccggcgag
     1921 gaaatctccg aagcgttact tcgcgatctg atcaacgatt cgtggaatct ggtggttgat
     1981 ggtctggcta aacgcgatca aaaaagagtg cgtccaggct aaagcgggaa tctatagcgc
     2041 atttttctcg cttaccattt ctcgttgaac cttgtaatct gctggcacgc aaaattactt
     2101 acacatggag tctttatgga tatcatttct gtcgccttaa agcgtcattc cactaaggca
     2161 tttgatgcca gcaaaaaact taccccggaa caggccgagc agatcaaaac gctactgcaa
     2221 tacagcccat ccagcaccaa ctcccagccg tggcatttta ttgttgccag cacggaagaa
     2281 ggtaaagcgc gtgttgccaa atccgctgcc ggtaattatg tgttcaacaa acgtaaaata
     2341 cttgatgcct cgcacgtcgt ggtgttctgt gcaaaaaccg cgatggacga tgcctggctg
     2401 aagctggttg ttgaccagga agatgccgat ggccgctttg ccacgccgga agcgaaagcc
     2461 gcgaacgata aaggtcgcaa gttcttcgcc tatatgcacc gtaaagatct gcatgatgat
     2521 gcagagtgga tggcaaaaca ggtttatctc aacgtcggta acttcctgct cggcgtggcg
     2581 gcgctgggtc tggacgcggt gcccatcgaa ggttttgacg ccgcaatcct cgatgcagaa
     2641 tttggtctga aagagaaagg ctacaccagt ctggtggttg ttccggtagg tcatcacagc
     2701 gttgaagatt ttaacgctac gctgccgaaa tctcgtctgc cgcaaaacac caccttaacc
     2761 gaagtgtaat tctctcttgc cgggcatctg cccggctatt tcccctcaga ttctcctgat
     2821 ttgcataacc ctgtttcagc cgtcatcata ggctgctgtt gtataaagga gacgttatgc
     2881 aggatttaat atcccaggtt gaagatttag cgggtattga gatcgatcac accacctcga
     2941 tggtgatgat tttcggtatt atttttctga ccgccgtcgt ggtgcatatt attttgcatt
     3001 gggtggtact gcggaccttc gaaaaacgtg ccatcgccag ttcacggctt tggttgcaaa
     3061 tcattaccca gaataaactc ttccaccgtt tagcttttac cctgcagggg attatcgtca
     3121 atattcaggc ggtattctgg ctgcaaaaag gcaccgaagc ggcagatatt ctgactacct
     3181 gcgcgcagtt gtggatcatg atgtatgcgc tgctttcagt cttctcgttg ctggatgtta
     3241 ttttgaatct ggcgcagaaa ttcccggcgg catctcagtt accgctgaaa gggatatttc
     3301 aggggattaa actgatcggc gcaatcctgg tcggcatttt gatgatctcg ctgctgattg
     3361 gtcagtcgcc agcgattcta atcagcggtc ttggtgcaat ggctgccgtg ctgatgttgg
     3421 tatttaaaga tccgattctt ggtctggtgg caggtattca gctttccgcg aacgatatgc
     3481 tgaaactggg cgactggctg gaaatgccga aatacggcgc ggatggcgcg gtgatcgata
     3541 ttgggttaac caccgtcaaa gtgcgtaact gggacaatac cattaccact attcccacct
     3601 ggtctctggt ttctgactcc tttaaaaact ggagcgggat gtcagcatct ggcgggcgac
     3661 gtattaagcg cagtatcagt attgatgtca ccagtattcg ttttcttgat gaagacgaaa
     3721 tgcaacgtct gaataaagcg catttgttaa agccttattt aaccagccgc catcaggaaa
     3781 ttaatgagtg gaatcgccag caaggttcta cggagtcggt attaaacctg cgccgaatga
     3841 ccaatattgg aacctttcgt gcctatctga acgaatatct gcgtaaccat ccgcggattc
     3901 gtaaagatat gaccttaatg gtacgccaac tggctccagg tgataacggt ttaccgctcg
     3961 agatctacgc gtttactaac accgtggtgt ggctggaata tgaaagcatt caggctgata
     4021 tattcgatca catatttgcg attgtcgaag agtttggtct gcgacttcat cagtcgccaa
     4081 ccggcaatga tattcgctct ctggcgggtg catttaagca gtaattaaaa aaaccgctct
     4141 catcgaatgg atgagagcgg acatcgtttt gtcggatgta gcgtgaatgc cttatttccg
     4201 acgcagcgtt ttaaatgcca caaacaggaa tacaatccac accggcagca ggatcgctga
     4261 caagcgcata tcatccatcg tgcacatcag cagcaaaatc atgccgagga aggcgatgca
     4321 aagatagttg ccgaacggat agagcagcgc cttaaactgt gtttcacgcc cctgacgtcg
     4381 catcgccgcg cgaaaacgca gatgcgccag gcagatcata atccagttca acagcaacgt
     4441 tgctaccacc agcgccatca gcagaccaaa cgctttttgc ggcagcagat agttgattaa
     4501 caccaccagc gaagtgatcg ctccggaaag catcagcgag ttaatcggca caccgcgacg
     4561 gctgacgcga gtcaaaaact tcggcgcatt accctgcaca gaaaggccaa acagcatgcg
     4621 gctgttagag taaaccccgc tgttatacac cgacagcgat gccactagaa tgacgaagtt
     4681 cagcgcagaa gctaccacat tgctgtcgag attatggaaa atcatcacaa acgggctact
     4741 gttggatttc acttccaccc acggatagag tgccagtaaa accaccagtg aaccgatata
     4801 aaacagcagg atgcgataca ccacctgatt taccgctttt ggaatgcttt tttccggatc
     4861 gcgcgcttca gcggctgtaa tcccaatcag ctccagaccg ccgaaggaga acataattac
     4921 cgccagcgac aaaatcagcc cattccagcc ggtggcgaag aaaccaccgt agcgccagag
     4981 gttgtcgata ctggctttct cgccgccgta accagaaaac agcagccaca ggccaaagcc
     5041 gatcataccg atgatcgcca gcactttaat cagcgcaaac cagaactcgg tttcgccata
     5101 taagcgcacg ttcaccaggt taacggcgtt gatgataata aagaaggcgg cagcccaaat
     5161 ccacgttgga acatccggga accagtactg catatagatg cccgcagcgg tcagctctgc
     5221 cattcccacc agcacgaaca ttacccagta gttccagcca gagaggaagc ccgcaaacgg
     5281 tccccagtat ttataggcaa agtgggcaaa tgaaccggat accggctctt cgaccaccat
     5341 ctcgccaagc tggcgcataa tcaggaaagc gatgatcccg gcgacgccgt agcccagcaa
     5401 tacagccgga cccgccatct gaatcgccgg gccaatgcca agaaacagac cagtaccaat
     5461 tgcgccaccc agcgcaatca gttgaatatg acggttatgt aatccgcgat gaagcgtcgg
     5521 ctcttgattc gacgcagtat cttccgatac ggttgacgcg tttttcacgc ctttcccctg
     5581 tgtgtctttt ttgttgaggg gcacctttta acatttagtg cccatcgtcg caagacacaa
     5641 tccacacggt taaacgaggt atcctgcttt aattccgtac ccgatgtcgg tgcagccaca
     5701 tcagcttata cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc
     5761 caatcatcgg tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa
     5821 tcggcagcag accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagga
     5881 ccgcgccgtg atacaacgcc tcatccagct tctgctcgct gaatgtttgt ggattattca
     5941 acgacggctc ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg
     6001 cggcgacccc ggcaagggca ataaagccgg tacccgtcgc cacagaaaga tgaaagccca
     6061 tccaccacag taaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca
     6121 acgcttcgcc cacccgacgg aatgccagat acaacagcac gaagatgatc atcagtgtca
     6181 tcggcaccat gagtttcagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga
     6241 atgccacgct ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt
     6301 gaaccaccga caccatgtca cgatcgcggg catcgatata aatccagctc gtcgggcgcg
     6361 cattctcggt tttcagcatc gacggtccgg tagagacttt aacgtcggcc acgtctgcca
     6421 gggtgatttg ctgcttcatc ggcgtcagga tcggcaactg gcgcagtgcc tgcggactat
     6481 cgcgccagct ttgcggataa cgcagattaa ttggataacg ggcaatccct tccaccgttt
     6541 cgccaaccat cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca
     6601 taccgtaacg tgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca
     6661 gccgctcagc aagcgcagaa gctacgcctg gcaccgttcg cgccacttct tcaatttgct
     6721 cagccatcgc gtcgatatcc gccagcacag tgccggaaac tttaatgccg atggggcttt
     6781 taatgccggt tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca
     6841 gccccggcag acgcacggtg ttatccagtt cctcaatgat tttgtccatt gtcatgcctg
     6901 gccgccactg atcctgtggc ttaagctgga tggtcgtttc taccatctcc agcggagcag
     6961 aatcggtggc ggtttccgct ttcccggttt tgccaaatac ccgcgccact tcaggtacgc
     7021 tcataattag cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg
     7081 gcagcgtcga tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc
     7141 caactttatt gagcggccag agaaccgtca gcaccgaaag cgccgccacc agcagcgtgg
     7201 ttttcggcca gtgcagtact ttcagcaaca gcggatgata aacacgaatc aaaaagcgat
     7261 tgagcggatt actgctttcc ggcggaattt tgccacggat ccagtaaccc atcaggatag
     7321 ggatcactac gatcgccagc agcgccgcac ccgccatcgc atacgttttg gtgaacgcca
     7381 acgggccaaa cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacaacg
     7441 tgataatcag cagactgata aacagcgccg gccccacttc aacagacgca tcggtgatca
     7501 cctgccagcg cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca
     7561 gctgtttatg cgcattctcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa
     7621 tcgcaatgcc acccagcgac ataatattgg cattcagccc ctggaagggc atgacaataa
     7681 aagcaataca caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc
     7741 agagaaacgg cgcgcagact accgccacca caataaactc ttccagcaac ttgccgctga
     7801 ggttgtcgat ggcccggtca atgagctggc tgcggtcgta tgttgtaact atctccacgc
     7861 cttccggcag actacttttc agcgtttcca gtttgtcctt cacggcggcg atcacttctc
     7921 gggcgttttt gccggatcgc aggatcacca ccccgcccgc cacttcgcct tcgccgttta
     7981 gttcggcaat gccccggcgc atcttcgggc caatctggac cttcgcaaca tcgcgcagat
     8041 aaacgggcac gccattttca ctggctttta aaacgatgtg attaaagtcg tcgagcgttt
     8101 gtagatagcc gctggcgcgc accatatatt ctgcttccgc cagttcgatt gacgaaccgc
     8161 ccgcttcctg gtttgaagca tccagcgcgc tttttacttc ggcgagactg atgccatact
     8221 gcgccaggcg ctgaggatcg ataaccacct gatactcttt caccacaccg cctaccgacg
     8281 ccacttccgc aacgtcagga atggttttca gctcatattt gagaaaccag tcctgcaatg
     8341 agcgtaaatc ggcaagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc
     8401 agccaacacc cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct
     8461 gtacctggtt gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtgccatctt
     8521 cgaaaatgac atacacataa gagtcgccaa actgcgagaa accacgcaca gtcttcgcgc
     8581 caggcaccga caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg
     8641 gtgcctggcc gggatagctg gttttaataa tcacctgcac atcggagaga tccggcaacg
     8701 catccactgg cgtattaatg atggtccagg tgccccagat gc
//
LOCUS       DAJTUP010000140         8515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTUP010000140 DAJTUP010000000
VERSION     DAJTUP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.142"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..959)
                     /gene="trkA"
                     /locus_tag="OO468_003565"
     CDS             complement(<1..959)
                     /gene="trkA"
                     /locus_tag="OO468_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR6260537.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKR"
     gene            complement(981..2270)
                     /gene="rsmB"
                     /locus_tag="OO468_003566"
     CDS             complement(981..2270)
                     /gene="rsmB"
                     /locus_tag="OO468_003566"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR6260538.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2316..3263)
                     /gene="fmt"
                     /locus_tag="OO468_003567"
     CDS             complement(2316..3263)
                     /gene="fmt"
                     /locus_tag="OO468_003567"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR6260539.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3278..3787)
                     /gene="def"
                     /locus_tag="OO468_003568"
     CDS             complement(3278..3787)
                     /gene="def"
                     /locus_tag="OO468_003568"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR6260540.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            3952..5076
                     /gene="dprA"
                     /locus_tag="OO468_003569"
     CDS             3952..5076
                     /gene="dprA"
                     /locus_tag="OO468_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR6260541.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5048..5521
                     /locus_tag="OO468_003570"
     CDS             5048..5521
                     /locus_tag="OO468_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR6260542.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5550..6092
                     /locus_tag="OO468_003571"
     CDS             5550..6092
                     /locus_tag="OO468_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR6260543.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6097..6669
                     /gene="tsaC"
                     /locus_tag="OO468_003572"
     CDS             6097..6669
                     /gene="tsaC"
                     /locus_tag="OO468_003572"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR6260544.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            6674..7492
                     /gene="aroE"
                     /locus_tag="OO468_003573"
     CDS             6674..7492
                     /gene="aroE"
                     /locus_tag="OO468_003573"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR6260545.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7489..7746
                     /locus_tag="OO468_003574"
     CDS             7489..7746
                     /locus_tag="OO468_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR6260546.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(7722..8276)
                     /locus_tag="OO468_003575"
     CDS             complement(7722..8276)
                     /locus_tag="OO468_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR6260547.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2114 a         2067 c         2146 g         2188 t
ORIGIN      
        1 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
       61 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
      121 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
      181 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
      241 ccaccaacca gcatgatccg tttatacggt ttttccagtc gctgtaattc actcatcacc
      301 gcgcggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc aacaatggtc
      361 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
      421 tgtggcatat gttcgcgcat ggtcgaaagt gcattaccaa tcagcgggcc gccataatag
      481 gctttaacta cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgcct
      541 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
      601 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgtacgta gtctggtgag
      661 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
      721 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
      781 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
      841 tgcaaggtcc gcagacgctc accgttggta tcgacaacag taatatcgtt gttctcgcca
      901 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
      961 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
     1021 tcttcggcac caggctgatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
     1081 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
     1141 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
     1201 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
     1261 atatctggat ggcgacgaat cacccccgtt gctgaacaag gcgcatctaa taaaatgcga
     1321 tcaaactgtt gctcgccaca ccattgggaa gggtaacggc catcaccttg tttcacggtt
     1381 gccttcatac caaggcgttt taaattgtcg taaatgcgag agaggcgctg ttcgtcaata
     1441 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
     1501 ggggcggcac aaagatccaa gatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
     1561 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
     1621 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
     1681 attcctgctt catccagcaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaata
     1741 cgcagccaca ttggcggacg ctggttattg gcttcggcga tggattgcca ctgctctgga
     1801 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcacgggta
     1861 ttaaactcgg ctaataactc ttcttgccga cgctggaact ggcgtaatac accgttaatc
     1921 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
     1981 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
     2041 acagtgcgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
     2101 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
     2161 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
     2221 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
     2281 atgctcggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
     2341 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
     2401 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
     2461 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga cgctttccag
     2521 actttaaccg gctgtccttc aatttccagc cagctcattg gccatgggtt gaaagcgcga
     2581 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
     2641 aacttctcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
     2701 gccaattgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
     2761 aacgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
     2821 gtgtctaaac caacatccat ttgcataatg gttacaccag tttctgcatc acccgcccat
     2881 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaatgttg
     2941 atacagccaa gacgcggcat ctccagcaca gctttaggca gaattaaacc ataggcgacg
     3001 acgaccataa catcagcctg caaatcggcg accagttgct ggttttcttg tggacgcagg
     3061 gaaacaggtt gaaaaacggg cagaccttct tcctcagcca gaactttaat cgggctgggc
     3121 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
     3181 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
     3241 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
     3301 ccagtttttc aactttctga cgaattcgtt gttgtttcag cggtgacaga taatccataa
     3361 acagtttgcc aaccaggtga tccatctcat gctgaataca gatagctaac agaccctctg
     3421 cttccagttc aaatggttta ccgtcgcggt caagggcgcg aattttaact ttctctgcgc
     3481 gcggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
     3541 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
     3601 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
     3661 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
     3721 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
     3781 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
     3841 caaatccccc tctgattgac agcatcactt gattgacagc atcacttgat tgacagcatc
     3901 actgaccaat cgcaaagatt gctaaggctt gcttatggca gggagataag gatggtcgat
     3961 atagaaattt ggctgcgttt aatgagcatc agcagcttgt acggcgatga tatggtccgt
     4021 atcgctcact ggctggcaaa acagtcgcat attgatgcgg ttgtattgca gcaaacaggg
     4081 cttacattgc ggcaggcaca acgctttctt tcatttccac gaaagagtat cgaaagctca
     4141 ctttgttggt tggagcaacc caaccatcat ttaatccctg cggacagcga attttatcct
     4201 cctcaacttc aggcgacgac agattacccc ggcgcactgt ttgttgaagg agaactgcac
     4261 gcgctgcatt catttcagct tgccgtagtg gggagtcggg cgcattcatg gtatggcgag
     4321 cgatgggggc gattattttg cgaaactctg gcgacgtgtg gagtgacaat tacgagtgga
     4381 ctggcgcgtg gaatcgatgg tgtggtgcat aaagcggcct tacaggtaaa tggcgtcagc
     4441 attgctgtat tggggaatgg acttaatacc attcatccgc gtcgccatgc ccgactggct
     4501 gccagtctgt ttgaacaggg tggcgctctc gtctcggaat ttcccctcga tgttccaccc
     4561 cttgcttaca atttcccacg aagaaatcgc attatcagtg gtctaagtaa aggtgtactg
     4621 gtggtggaag cggctttgcg cagtggttcg ctggtgacag cacgttgtgc gcttgagcag
     4681 gggcgtgaag tttttgcctt gccaggtcca atagggaatc cgggaagcga agggcctcac
     4741 tggttaataa aacaaggtgc gattcttgtg acggaaccgg aagaaattct ggaaaacttg
     4801 caatttggat tgcactggtt gccagacgcc cctgaaaatt cattttattc accagatcag
     4861 caagacgtgg cattgccatt tcctgagctc ctggctaacg taggagatga ggtaacacct
     4921 gttgacgtcg tcgctgaacg tgccggccaa cctgtgccag aggtagttac tcaactactc
     4981 gaactggagt tagcaggatg gatcgcagct gtacccggcg gctatgtccg attgaggagg
     5041 gcatgccatg ttcgacgtac taatgtattt gtttgaaacc tatattcaca ctgaagctga
     5101 gttgcgtgtg gatcaagaca aacttgaaca ggatcttacc gacgcaggtt ttgagcgaga
     5161 agatatctac aatgccctgc tatggctgga aaaacttgct gattatcagg aagggttggc
     5221 agaaccgatg caactggcct cggatcctct ctccatgcgt atttatacac cggaagagtg
     5281 tgaacgactg gatgccagct gccgtgggtt tctgcttttc cttgagcaga ttcaggtgct
     5341 caaccttgaa actcgtgaaa tggtgataga gcgagtgctc gcgctggata acgcagagtt
     5401 cgaactggat gatctgaaat gggtgatcct gatggtgttg ttcaacatcc cgggctgcga
     5461 aaatgcgtac cagcaaatgg aagaattact ctttgaagtg aatgaaggta tgctgcatta
     5521 atcatttctt taattcagca taagttgtta tggcaaaatc agcactgttc acggtgcgta
     5581 ataatgagtc ctgcccaaag tgcggggctg aactggttat tcgatccggg aaacacggtc
     5641 cgtttcttgg atgctcacag tatccggcgt gtgactacgt ccgtcctctg aaatcttcag
     5701 cggatggaca tattgtcaaa gttctggagg ggcaggtttg ccccgtatgt ggcgcaaatc
     5761 tggtattacg ccagggacgc tttggtatgt ttattggttg cagtaactac cctgaatgcg
     5821 aacataccga acttatcgat aaaccggacg aaacagcaat tacatgcccc caatgtcgga
     5881 cgggccatct ggtccagcgc cgctcccgtt atggcaaaac atttcactcc tgtgatcgct
     5941 acccggagtg tcaatttgcc attaacttca aaccaatagc tggagaatgc cctgagtgtc
     6001 attatccgct actcatcgaa aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta
     6061 aacaatgtgg aaagccggtt tcggcggaat aataacgtga ataataacct gcaaggagac
     6121 gctatcgcag ctgcgataga tgtcctcaat gaaaaacgtg tcatcgccta tccaacggaa
     6181 gccgttttcg gtgtcgggtg cgatcctgat agcgaaacag cagtgatgag actgttggag
     6241 ttaaaacagc gtccggttga taaggggctg attttaatcg cggcaaatta cgagcagctt
     6301 aaaccctata ttgatgacac catgttgact gacgtgcagc gtgaaaccat tttttcctgc
     6361 tggccaggtc ctgtcacctt tgtctttccc gcgcctgcga caacaccgcg ctggttgacg
     6421 ggccgctttg attcgcttgc tgtacgagtc accgaccatc cgttggtggt tgctttgtgc
     6481 caggcttatg gtaaaccgct ggtttctacc agtgccaact tgagtggatt gccaccttgt
     6541 cgaacagtag acgaagttcg cgcacaattt ggcgcggcgt tcccggttgt gcctggtgaa
     6601 acgggggggc gtttaaatcc ttcagaaatc cgcgatgccc tgacgggtga actgtttcga
     6661 caggggtaac ataatggaaa cctatgctgt ttttggtaat ccgatagccc acagcaaatc
     6721 gccattcatt catcagcaat ttgctcagca actgaatatt gaacatccct atgggcgcgt
     6781 gttggcaccc atcaatgatt tcatcaacac actgaacgct ttctttcgtg ctggtggtaa
     6841 aggtgcgaat gtgacggtgc cttttaaaga agaggctttt gccagagcgg atgagcttac
     6901 tgaacgggca gcgttggctg gtgcagttaa taccctcaag cggttagaag atggacgcct
     6961 gctgggtgac aataccgatg gtgtaggctt gttaagcgat ctggaacgtc tgtcttttat
     7021 ccgccctggt ttacgtattc tgcttatcgg cgctggtgga gcatctcgcg gcgtactact
     7081 gccacttctt tccctggact gtgcggtgac aatcactaat cggacggtat cccgcgcgga
     7141 agagttggct aaattgtttg cgcacactgg cagtattcag gcgttgggta tggacgaact
     7201 ggaaggtcat gagtttgatc tcattattaa tgcaacatcc agtggcatca gtggtgatat
     7261 tccggcgatc ccgtcatcgc tcattcatcc aggcatttat tgctatgaca tgttctatca
     7321 gaaaggaaaa actccttttc tggcatggtg tgagcagcga ggctcaaagc gtaatgctga
     7381 tggtttagga atgctggtgg cacaggcggc tcatgccttt cttctctggc acggtgttct
     7441 gcctgacgta gaaccagtta taaagcaatt gcaggaggaa ttgtccgcgt gaatcaggcc
     7501 atccagtttc cggacaggga agagtgggac gagaataaaa aatgtgtatg ttttcccgct
     7561 ctcgtgaatg gtatgcaact gacatgcgcg atctctggcg agagtctggc gtatcgcttt
     7621 actggagata cgccagaaca gtggttagcg agttttcgtc agcatcgctg ggacctggaa
     7681 gaagaagcgg aaaacttaat tcaggaacaa agtgaagatg atcaaggctg ggtctggtta
     7741 ccctgatcca gatattcgtc cttccatttc acgtaattat tcgcggaata gcgtaaccca
     7801 gccttctctt catcacttaa cgggcggatc tgtttgacgg ggctaccgag atacagatat
     7861 ccgctctcca gccgtttatt ttgtgggacc agactacccg caccaatcat cacatcatct
     7921 tctactattg cgccatcaag taaaattgag cccatcccaa ccaaaactcg attgccaatg
     7981 gtgcagccgt ggagcatcac cttgtgacca acagtgacat cttcgccaat ggttaatggg
     8041 ttgccatctg ggttgtacga ggatttatga gtgacatgca acatactgcc atcctggata
     8101 ttggtgcgtg ctccgatctg tacataatgt acatctccac gaatcacaac gagcggccag
     8161 atccccacat catcagccag acgaacgtca ccaatcacga cactgctatc gtcgatcatt
     8221 acgcgctgac cgatttgtgg aaaaagatcg cggtatgggc gtaaaacatc agacatactt
     8281 acctcagcaa taaatgattt actaatgact ttgggggcat tattggcctt gtgcaagtct
     8341 tttagtatgc aaaaaagcac cgttttgtgt gcgattgcag caaaaagggt gaaaaaacaa
     8401 caaacagaaa agaagatcaa aaaaatactt gtgcaaaata ttgggatccc tataatgcgc
     8461 ctccgttgag acgacaacgt gaaacacttc acaggatggt cggaacaacg aagag
//
LOCUS       DAJTUP010000141         8509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTUP010000141 DAJTUP010000000
VERSION     DAJTUP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.143"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(756..1952)
                     /gene="hemY"
                     /locus_tag="OO468_003576"
     CDS             complement(756..1952)
                     /gene="hemY"
                     /locus_tag="OO468_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR6260548.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1955..3160)
                     /gene="hemX"
                     /locus_tag="OO468_003577"
     CDS             complement(1955..3160)
                     /gene="hemX"
                     /locus_tag="OO468_003577"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR6260549.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3182..3922)
                     /gene="hemD"
                     /locus_tag="OO468_003578"
     CDS             complement(3182..3922)
                     /gene="hemD"
                     /locus_tag="OO468_003578"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR6260550.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3919..4860)
                     /gene="hemC"
                     /locus_tag="OO468_003579"
     CDS             complement(3919..4860)
                     /gene="hemC"
                     /locus_tag="OO468_003579"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR6260551.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5247..7793
                     /gene="cyaA"
                     /locus_tag="OO468_003580"
     CDS             5247..7793
                     /gene="cyaA"
                     /locus_tag="OO468_003580"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR6260552.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7833..8153)
                     /gene="cyaY"
                     /locus_tag="OO468_003581"
     CDS             complement(7833..8153)
                     /gene="cyaY"
                     /locus_tag="OO468_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR6260553.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8201..>8509)
                     /locus_tag="OO468_003582"
     CDS             complement(8201..>8509)
                     /locus_tag="OO468_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6260554.1"
                     /translation="LVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDD
                     RNVYTWTDTEVRITPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP
                     "
BASE COUNT         1933 a         2209 c         2191 g         2176 t
ORIGIN      
        1 tctgctgcgc tggccatgag aggaagagcg gcggcgacag caacaacaag acgtttgggt
       61 gaaaacgaaa attccatgca aaatgctccg gtttcatgtc atcaaaatga tgacgtaatt
      121 aagcattgat aattgagatc cctctccctg acaggatgat tacataaata atagtgacaa
      181 aaataaatta tttatttatc cagaaaatga attggaaaat caggagagcg ttttcaatcc
      241 tacctctggc gcagttgata tgtaaggcag gtttattata tcgcgttgat tattgatgct
      301 gtttttagtt ttaacggcaa ttaatatatg tgttattaat tgaatgaatt ttatcattca
      361 taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt tattactcag
      421 gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt atttcccgat
      481 tctctgtggc ataataaacg agtagatgct cattccatct cttatgttcg ccttagtgcc
      541 tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact gacagatgtc
      601 gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac ttgttgtcat
      661 acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat gcatcagaaa
      721 ggtcccggag gtgctttcct ccgggtgaga aggaactact gtggcgggtt attctgcaac
      781 gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc
      841 agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg
      901 aaggcgagcg atgcttcctg ccattcaccg tgcttcatca gtgactgacc caatgtgctc
      961 cacaacagcg ggcgatcacc gacgtttttg atttgctggc gtagcacttt ttccagctgt
     1021 tccgggttat tggttttcag acgcgggatc ggcagcagta ggcgatcgtc atactggcgt
     1081 ttcaggccgt cgatgataat ttgctgggca gtatcatgat cgtcacattc aataagatgt
     1141 tccgccattg ccacctgcaa cgctacctga tgacgcgttt tccggctttg gtttttccac
     1201 cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag gccaatccat
     1261 gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt cgccattgat
     1321 gggatgatat ccagcagaga actccatgcc cctgtgcgga tatacgcctg ttccgccaga
     1381 cgtaatactt caggatggcg tggcgtaact tccagcagct tatccacgcc gtggcgtgca
     1441 gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac cggaatggtg
     1501 tcattgccgg ccagctccgc tgcgcgttcc agatgttggt tggcgcgtgc ttcatcacca
     1561 cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc agcgtgatcg
     1621 gcatttttcg ccatcagctt ttcaacttgc tgataatcac cttccgccag tttcagcagc
     1681 gcctgttcgg tctgcttacg cgcacggcga cgcttacgtc cgacaaacca tccacgggtg
     1741 tgcgcgccag tgcggaagat ccgccgcagt agccactcaa tggcaaacag cactaccatc
     1801 gccagaatca atatgatcgc caggcccgtg acgctggttt cgatattgta gttgtcggtc
     1861 tggatcagca cataaccctg atgaccggca atcatcgggc caaccactat ccccgcaatc
     1921 agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc gcggctgccg
     1981 gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtttagctt
     2041 ccgttgtccc cgctgccggt tgtgccagca ggttacgcac gcgagtctgc atcagttttt
     2101 ccagcatcgc ctggctttgc agggtttccg gaagatccat cgagatattt tgctggctta
     2161 actggtccac ctcgtcgagg aacgctttgg tggtggcatc atcagtatcg tagtaagcac
     2221 gtacccaggt ggagacgttc tccagcgcct ggcgataagt ctcttcctga tgacgcggta
     2281 cagcttgtgc tgcgaccagc aggcgagagc gaatattttc gcgcagatag atatcctgat
     2341 ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg aatcgtaatg aagttgtcca
     2401 taaagttctg ccagcttttt tgcagattga tacgccattc gctgatggaa ctggaaagct
     2461 cttcaccgtc tgaatccatc ggcgaaccat cgctgtcatt atcggccaga cgcaggttat
     2521 ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc gtcataatcc acctgcgata
     2581 ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg aacggtaatc agactcgggt
     2641 cattcatatc cgccaggctg gcgtctgcac ttttcagcaa cgctgcagag gtcgtgacgt
     2701 cctgatcgct ccacagcttc cgtccggcga gtttcaccag aaaatcggcc tgagccagca
     2761 gccaggtttt agcatcgctg ccggaaatgg tggcgatctt ttgttggact tcatccaact
     2821 gttttgccag cgtttcttgc tgacgattcg cctgcttaag ctgcgcagct tgttgcttaa
     2881 taatgccttc cagctcggct ttttggctct cctgggcttt ttgcaatgcc gtcagttggt
     2941 tagccagggc atcgctggtg gcggtctgat tgacggcctg ttgtttaccc cagccataca
     3001 aaccgacacc cgccgccaga gcaatagcga tagccaccgc gctgagaatc aatgcagtat
     3061 tgttcttact ctttttttct gttgcgacag gttgtgacgt ggtgtccacg gcctccctgg
     3121 tctcttcaac cacggcggag gttttttctt gttccgtcat tatggcttcc tgttatgaga
     3181 gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt atcggcgacc ttaatgtctt
     3241 gccagcccag ttcccgggcg agtttcgcca aacgctcact gacgaccaat agtcgacagt
     3301 gtagtaacca gtgctcacga taccattgtg ggatcagcga ccagagttgc tgcaacattt
     3361 caccgctggt aacaacgacc atcgtcacct cgcgggattg ccagcgcatc gcttcttctg
     3421 caccatcgta atggattgcg catcgttgat aacattcaca aaaagtgacc tcagcaccgc
     3481 gcaccgtcag ggtatcccca attagctcac gaccgccatt gccacgtaat atcagcgcac
     3541 gtttgcccgc aatattttgt aattcaggta attgtagcaa gacttcgctg atttcccgat
     3601 cctgtgggta gagaatcttc tgtccgctta cggtatgtag cgccagtgcg gtggtgcgtc
     3661 caatggcgaa ataagtaggt agttggggcc atttacgatc ttgctgatgc agctgtgatt
     3721 gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag atcgctctcc cccagcgctg
     3781 ccagttgatc agcaagttgc ggtaattgtc gacccggaga aaactcaatc agcggaaaat
     3841 gccaggccac ctgccccagt gtgcgcagac ggctcactaa ctcttctcca gcgggagacg
     3901 ggcgggtgac caggatactc atgccggggc ctctccgtta tagacttcag cgaggatctc
     3961 gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc cccatttgtt cggcatcttg
     4021 cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg tccggcgcgc cgaccagcgc
     4081 acatagccag atttcgccat caataagctc ggcgtagcta ccaattggca cctgacatcc
     4141 gccttcgaga cgggtattca tggcgcgttc tgcggtaacg cgcagtgcag tttcgtggtg
     4201 attcagcgcg gcaagcagct cgcgagtgcg tgtatcatca aggcggcatt caataccgac
     4261 tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt aacgcggcgc gaatgcggga
     4321 ctccagacct aaacgtttta gtccggctac ggcaagaatg atggcatcgt attcgccgtt
     4381 atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg gagcggataa tcagatccgg
     4441 gcggcgttca gccagttggc actggcgacg taaactggac gtcccgacga tactgcctgc
     4501 tggtaacgca tccagactgt catagttatt ggacacaaag gcatcgcgag gatcttcacg
     4561 ctcgcaaata gtgaccagcc ccagaccttg cgggaattca accggcacat ctttcattga
     4621 atgtacggcg atatcagcgc gattctccag gagcgcgact tccagctctt ttacaaataa
     4681 gccttttccg cctactttcg ccagcggcgt atcaagaatc acatcgccgc gcgtcaccat
     4741 cggtaccagt tcaacgacca ggcccggatg gctcgccatc aacttgtctt tgacatagtg
     4801 tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt cttaaaacat tgtctaacat
     4861 gcttgttacc gtcattatca tccgtggtcc atcctaacat ccttgccaga gtgatgtcag
     4921 tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat gaggtctggc agtggatcct
     4981 gacaggcgtt tcacgccgtc gtaataagga atttacagag aataaacggt gctacacttg
     5041 tatgtagcgc atctttcttt acggtcaatc agcaaggtgt taaattgatc acgttttaga
     5101 ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa agtggtaacg gttacctttg
     5161 acatacgaaa tatcccgaat gccgcgtgtt accgttgatg ttggcggaat cacagtcatg
     5221 acgggtagca aatcaggcga tacgtcttgt acctctatat tgagactctg aaacagagac
     5281 tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc tgctatgggg cctgcattcc
     5341 aacaggtcta cagtctactg ccgacattgt tgcactatca ccatccgcga atgccgggtt
     5401 accttgatgg taacgttccc aaaggcattt gcctttacac gcctgatgaa actcaacgcc
     5461 actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt acaggatcca ccgaaaggtg
     5521 agcttccaat tactggtgta tacaccatgg gcagcacctc gtccgtaggg caaagttgtt
     5581 cctctgacct ggatatctgg gtctgtcatc aatcctggct cgatagcgaa gagcgccagt
     5641 tactacaacg taaatgcagc ctgctggaaa gctgggccgc ctcgctgggt gtggaagtca
     5701 gcttcttcct gattgatgaa aaccgcttcc gtcataatga aagcggcagc ctggggggcg
     5761 aagattgtgg ctccacccag catatactgc tgcttgacga attttatcgt actgccgtgc
     5821 gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg cgacgaagaa gagcattacg
     5881 acgactatgt gatgacgctt tacgcgcagg gcgtgctgac gccaaatgaa tggctggatc
     5941 tcggtggctt aagctcgctt tctgctgaag agtactttgg tgccagcctt tggcagctct
     6001 acaagagtat cgattcccca tacaaagcgg tgctgaaaac actgctgctg gaagcctatt
     6061 cctgggaata cccgaaccca cgtctgctgg cgaaagatat caaacagcgt ttgcacgacg
     6121 gcgagattgt atcgtttggc ctcgatccat actgcatgat gctggagcgt gttactgaat
     6181 acctgacggc gattgaagat ttcacccgtc tggatttagt acgtcgctgc ttctatttaa
     6241 aagtgtgcga aaaactcagc cgtgaacgcg cctgtgtagg ctggcgtcgc gcagtgttga
     6301 gccagttagt gagcgagtgg ggttgggacg aagctcgtct ggcaatgctc gataaccgtg
     6361 ctaactggaa gattgatcag gtgcgtgagg cgcacaacga gctgctcgac gcgatgatgc
     6421 agagctaccg taatctgatc cgctttgcgc gtcgcaataa ccttagcgtc tccgccagtc
     6481 cgcaggatat cggcgtactg acgcgtaaac tgtatgccgc gtttgaagca ttgccaggta
     6541 aagtgacgct ggtaaacccg cagatttcac ccgatctctc ggaaccgaat ctgaccttta
     6601 tttatgtgcc gccgggccgg gctaaccgtt caggttggta tctgtataac cgcgcgccaa
     6661 atattgagtc gatcatcagc catcagccgc tggaatataa ccgttacctg aataaactgg
     6721 tggcgtgggc atggtttaac ggcctgctga cctcgcgcac ccgtttgtat attaaaggta
     6781 acggcattgt cgatttgcct aagttgcagg agatggtcgc cgacgtgtcg caccatttcc
     6841 cgctgcgctt acctgcaccg acaccgaagg cgctctacag cccgtgtgag attcgccatc
     6901 tggcgattat cgttaacctg gaatatgacc cgacagctgc gttccgcaat caggtggtgc
     6961 attttgattt ccgtaagctg gatgtcttca gctttggcga gaatcaaaat tgcctggtag
     7021 gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt gcgtacgctg cacttcaacg
     7081 gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg caaaatgcat caggacgccg
     7141 caccgccaga tagcgtggaa gtcttctgtt atagccagca tctgcgcggc ttaatccgta
     7201 ctcgcgtgca gcaactggtt tctgagtgta ttgaactgcg cctttccagc acccgccagg
     7261 aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac ctggggcttg ttcttcgaac
     7321 gcctgaatgt atcggtacag aagctggaga acgccatcga gttttatggc gctatttcgc
     7381 ataacaaact gcacggcttg tcagtgcagg ttgaaaccaa tcacgtcaaa ttaccggcgg
     7441 tggtggacgg ctttgccagc gaagggatca tccagttctt tttcgaagaa acgcaagacg
     7501 agaatggctt taatatctac attctcgacg aaagcaaccg ggttgaggtg tatcaccact
     7561 gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg cttctactcg tcatcgcacg
     7621 accgcttcac ctacggctca agcttcatca acttcaacct gccgcagttc tatcagattg
     7681 tgaaggttga tggtcgcgaa caggtgattc cgttccgaac aaaatcgatt ggcaacctgc
     7741 cgcccgccaa tcaggatcac gatacgccgc tattacagca gtatttttcg tgatgagcgt
     7801 gccggaaagc gaggcttatc cggcatgcaa tcttagcgga aactgactgt ttcacccgcc
     7861 tgttgcgtcg ccgcctgttc cagcaaatcc cagaaggttt cgccgctgcg atcgcaaatc
     7921 cattcatcgc ctttcaggtc aaaatggtag ccgccctgtt tggttgccag ccacacctgg
     7981 tgcagcggct cctggcggtt aataatgatt ttgctgccgt tttcaaaggt aatggtcagt
     8041 acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat ccctgtcgtc cagacgctct
     8101 tcaatggtca gccacagttg atcagccagg cgatgaaatt cactgtcgtt cattgttgta
     8161 tcctgttttt aagtgatggc ggcagtatag cggcatgggg tcagggcttc aaagtttgca
     8221 cctctgctgc tgcgttccgg catgattcat ccgtcaccgg aataatgatg tctctgtttt
     8281 gaacttcacg tacgttctcc tctctgacac gagttttcgc cgggcagggg gagggcgtaa
     8341 tccttacctc tgtatcggtc catgtataaa cattgcgatc gtcataagcg aagcgatcgt
     8401 ttttagttag cgaaccgtta actggaataa tttgcaagct ctttgaccgt aattggcgga
     8461 taaagtaagc atgctgtttg ccgctattaa atgagcgatc aatgaccag
//
LOCUS       DAJTUP010000142         8476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTUP010000142 DAJTUP010000000
VERSION     DAJTUP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.144"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..234
                     /locus_tag="OO468_003583"
     CDS             <1..234
                     /locus_tag="OO468_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6260555.1"
                     /translation="AGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVV
                     DTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            486..1403
                     /gene="oxyR"
                     /locus_tag="OO468_003584"
     CDS             486..1403
                     /gene="oxyR"
                     /locus_tag="OO468_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR6260556.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(1386..2786)
                     /gene="sthA"
                     /locus_tag="OO468_003585"
     CDS             complement(1386..2786)
                     /gene="sthA"
                     /locus_tag="OO468_003585"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR6260557.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3107..3757
                     /gene="fabR"
                     /locus_tag="OO468_003586"
     CDS             3107..3757
                     /gene="fabR"
                     /locus_tag="OO468_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR6260558.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3757..4116
                     /locus_tag="OO468_003587"
     CDS             3757..4116
                     /locus_tag="OO468_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR6260559.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4156..5256)
                     /gene="trmA"
                     /locus_tag="OO468_003588"
     CDS             complement(4156..5256)
                     /gene="trmA"
                     /locus_tag="OO468_003588"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR6260560.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      5370..5560
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5625..7469
                     /gene="btuB"
                     /locus_tag="OO468_003589"
     CDS             5625..7469
                     /gene="btuB"
                     /locus_tag="OO468_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR6260561.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            7414..8271
                     /gene="murI"
                     /locus_tag="OO468_003590"
     CDS             7414..8271
                     /gene="murI"
                     /locus_tag="OO468_003590"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR6260562.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1965 a         2059 c         2258 g         2194 t
ORIGIN      
        1 gcgggaacgt ctgcatgggg actggtgcag gtgatggtgt cagaaacaaa agtggcttcc
       61 atcgccggga ttcaaaactt tggcagtttt gtttttgctt cctttgctcc gatcgtaacc
      121 ggttgggtag tggataccac acactcgttt aatctggcgc tggttatcgc ggcctgtgtg
      181 acgttcaccg gggcgctgtg ttacttcttt attgtcaaag atcgcattga gtaatcgtta
      241 ctatcaggct ctcttgctgt gggcctgtag aataaaaaaa agcggatcct ggagatccgc
      301 aaaagttcac gttggcttta gttattcgag ttgagaaact ctcgaaacgg gcagtgactt
      361 caagggttaa aagaggtgcc gctccgtttc tgtgagcaat tatcagtcag aatgcttgat
      421 agggataatc gttcattgct attctaccta tcgccatgaa ctatcgtggc gatggaggat
      481 ggataatgaa tattcgtgat cttgagtacc tggtggcatt ggctgaacac cgccattttc
      541 ggcgtgcggc agattcctgc cacgttagcc agccgacgct tagcggccaa attcgtaagc
      601 tggaagatga gctgggcgtg atgttgctgg agcggaccag ccgtaaagtg ttgttcaccc
      661 aggcgggaat gctgctggtg gatcaggcgc gtaccgtgct gcgtgaggtg aaagtcctta
      721 aagagatggc aagccagcag ggtgagacga tgtccggacc gctgcatatt ggtttgattc
      781 ctacggttgg accatacctg ctaccgcata ttatcccgat gctgcaccag acatttccaa
      841 agctggaaat gtatctgcat gaagcacaga cccaccagtt actggcgcaa ctggacagcg
      901 gcaaactcga ttgcgtgatc ctcgcgctgg tgaaagagag cgaagcattc attgaagtgc
      961 cgttgtttga tgagccaatg ttgctggcta tctatgaaga tcacccgtgg gcgaaccgcg
     1021 aatgcgtact gatggccgat ctggcagggg aaaaactgct gatgctggaa gatggtcact
     1081 gtttgcgcga tcaggcaatg ggtttctgct ttgaagccgg ggcggatgaa gatacacact
     1141 tccgcgcgac cagcctggaa actctgcgca acatggtggc ggcaggtagc gggatcactt
     1201 tattgccagc gctggctgtg ccgccggagc gcaaacgcga tggggttgtt tatctgccat
     1261 gcattaagcc ggaaccatgc cgcactattg gcctggttta tcgtcctggc tcaccgctgc
     1321 gcagccgcta tgagcagctg gcagaggcca tccgcgcaag aatggatggc catttcgata
     1381 aagtgttaaa acaggcggtt taaaccgttt aacgcagcta cccgataggc ttccgccatc
     1441 gtcgggtagt taaaggtggt gttgacgaag tactcaatag tgttgccgcc acctttctgt
     1501 tccataatcg cctgaccgat atgaataatt tcggcagcgc gctcgccaaa gcagtgaata
     1561 cccagaatct cttttgtttc ccgatggaac aaaattttca gcgtgcccac gttcatgccg
     1621 acgatttgtg cacgtgccag atgtttgaac tgggcgcggc ccacttcata cggcactttc
     1681 atcgcggtca gctgctgttc ggttttgccc acagagctga tttccgggat ggtgtaaatg
     1741 ccggtaggga tatcttcaat cagatgtgcg gtggcttcgc cttttaccag cgcctgcgcg
     1801 gcaatgcgcc cctggtcata ggccgctgat gccaggctcg gataaccaat cacgtcgccc
     1861 accgcgtaaa cgtgcggctg tgcggtctga tacatgctgt tgaccttcag ctgtccgcga
     1921 ctgtcagttt ccagcccaat gttctgtaac gccagcgaat cggtattacc ggtacgaccg
     1981 ttggcataga gcaggcagtc agctttcagt tttttacccg acttcagatg catgatcaca
     2041 ccgtcgtcac agctttcgat cttctcgtac tcttcgttgt gacgaatcac tacgccactg
     2101 ttccagaagt ggtaggagag agaatctgac atctcttgat cgagaaatgc cagcaggcga
     2161 tcgcgggtgt tgatcagatc cacttttaca tccataccgc ggaagatcga cgcatattca
     2221 cagccgatca ctccagcacc atagataagt acatggcgcg gttcgtggtg catgctaaga
     2281 attgagtcgc tgtcgtaaat gcgtggatgg gtgaaatcaa catctgttgg atgatatgga
     2341 cgagagccgc aggcaataac aaatttttca gcggttagtg tttcaacgct gccgtccggg
     2401 caatccagcg ccaacgtatg ctcgtcaaca aagcgagcgt tcccctgcaa tatttcacag
     2461 tgattacgtt cgtaaaatcc ctgacgcatg cgcgtttgtt gattaatcac gttatcggca
     2521 tggttaagga tatcggcaaa agaagagcgg agcagtcggg aatggtcgct gtatagtggg
     2581 ttttgattga attctataat gcggctgacg gcgtgacgga gagctttcga cgggatggtg
     2641 ccccagtggg tgcaaccgcc gccaacattt tgataacgct cgataactgc gacgcgcgca
     2701 ccttgcttaa ccaggcccat tgcagcgcct tcgccgccgg ggccggaacc tattactatg
     2761 gcatcgtaat cgtaggaatg tggcatggta gggcttacct gttcttatac ataaaagcaa
     2821 cagaatggta acattctatc gcgggtaagc caattgatcc ccgtcattta tctggctata
     2881 tcctgagcgg cctttgcttt gtctgtttct tacttttgcc ctgacgtttt attggatttt
     2941 tatcgacgat actctccgtt taagcggcag gtttccgctg tacgtaaaag aaccggccag
     3001 taaatatgtt ttattgcgtt accgttcatt cacaatactg gagcaatcca gtatgttcat
     3061 tctctggtat agtgccagca gtacttttgg caaggattca gacatcgtga tgggcgtaag
     3121 agcgcaacaa aaagaaaaaa cccgccgttc gctggtggaa gccgcattta gccaattaag
     3181 tgctgaacgc agcttcgcca gcctgagttt gcgtgaagtg gcgcgtgaag cgggcattgc
     3241 tcccacctct ttttatcggc atttccgcga cgtagacgaa ctgggtctga ccatggttga
     3301 tgagagcggt ttaatgctac gccaactcat gcgccaggcg cgtcagcgta tcgccaaagg
     3361 cgggagtgtg atccgtacct cggtctccac atttatggag ttcatcggta ataatcctaa
     3421 cgccttccgg ttattattgc gggaacgctc cggcacctcc gctgcgtttc gtgccgccgt
     3481 tgcgcgtgaa attcagcact tcattgcgga acttgcggac tatctggaac tcgaaaacca
     3541 tatgccgcgt gcgtttactg aagcgcaagc cgaagcaatg gtgacaattg tcttcagtgc
     3601 gggtgccgag gcgttggacg tcggcgtcga acaacgtcgg caattagaag agcgactggt
     3661 actgcaactg cgaatgattt cgaaaggggc ttattactgg tatcgccgtg aacaagagaa
     3721 aaccgcaatt attccgggaa atgtgaagga cgagtaatga aacaagcaaa tcaagataga
     3781 ggtacgctgc tgctggcgtt ggttgctggt ttatcgatta atggtacttt cgcagcgctg
     3841 tttagctcca ttgtgccatt ttctgtattc ccgattattt ccctggtgct gacggtttac
     3901 tgcctgcatc aacgttatct taatcgcacc atgtcggtag gcttgccggg tctggcagct
     3961 gcctgtttta ttctcggcgt actgctgtac agcacggtag ttcgtgcgga atatccggat
     4021 atcggctcta acttcttccc ggcagtactc tccgtcatta tggtgttctg gattggcgcg
     4081 aagatgcgta accgtaagca ggaagttgct gagtaatcgg tatcatgccg gatgctggcg
     4141 catccggcat gggttttact tcgcggtcag taatacgccg cactccatat ggtgcgtgta
     4201 ggggaactga tcaaacagag ccagacgttc gaccttgtgc gtctggctta atgtttccag
     4261 atttttgcat aacgtttccg gattgcagga gatatacaga atacgtggat acgcctgcac
     4321 cattttctcg gtttcactgt ccagaccgct gcgcggtggg tcgacaaaaa tggtttcgaa
     4381 ctgatagctc tttaagtcga tcccttgcag gcggttaaac tcgcgcacac cattcatcgc
     4441 ctgagtaaat tcttctgccg ccatacgaat aatttgtacg ttatcaatat ggttagcggc
     4501 aatgttgtat tgcgcggcag cgactgacgg tttggcgatt tcggtggcta atacccgatc
     4561 aaaattgcgc gccagcgcta atgaaaagtt accgttgccg cagtacagct ccagtaaatc
     4621 gccttttgag cctttggtta cgtccagcgc ccattccagc atctgaatat tcatcgccgc
     4681 gttcggctgg gtaaagctgt tttctacctg acgatagatc atctctttcc ctgcgaccgg
     4741 cagacgctcg tcgatgtaat cttgatccag cgcgattttg gttttcgttg cccgaccaat
     4801 cagatgcaca ttcagattct gcgcgcgcag tgcatcgcgc agtgcttccg cttcctgacg
     4861 ccactcatca tccagctttt tatggtacag cagggaaacc accgcctgat tactcagcgt
     4921 ggtgaggtaa tcaatctgga acaacttgtg gcgcagaacg ggattattac gcacacccgc
     4981 aatcatcgcc gtcatcaact ggttgatgag ttcactggcg gcggggaagc tatccacgcg
     5041 gatgcggctt ttggtttgtt gatcgaaaat gatgtgatac aggtcatcgc catcgtgcca
     5101 gatgcggaac tccgcgcgca tccggtaatg actgaccggc gagcgaaaca cttccggaac
     5161 caggtcagaa aacggtgcca tcatactttg caaacgtacc actttttcgg ctaactgcgc
     5221 ttcatactgt tctgttggaa ggtgttcggg ggtcatgatg tatcctgaaa aattaaagta
     5281 cgcggcgatt gtagggattg ctcatcagat gtccagatct tgatgaattc ctatttgtga
     5341 gctacgtctg gacagtaact tgttacaacc tgtagcatcc acttgccggt cctgtgagtt
     5401 aatagggaat ccagtgcgaa tctagagctg acgcgcagcg gtaaggaaag gtgcgatgat
     5461 tgcgttatgc ggacactgcc atccggtggg aagtcatcat ctcttagtat cttagatacc
     5521 cctccaagcc cgaagacctg ccggccaacg tcgcatctgg ttctcatcat cgcgtaatat
     5581 tgatgaaacc tgcggcatcc ttcttctatt gtggatgctt tacaatgatt aaaaaagctt
     5641 cgctgctgac ggcgtgttcc gtcacggcat tttccgcttg ggcacaggat accagcccgg
     5701 atactctcgt cgttactgct atccgttttg aacagccgcg cagcactgtg cttgcaccaa
     5761 ccaccgttgt gacccgtcag gatatcgacc gctggcagtc gacctcggtc aatgatgtgc
     5821 tgcgccgtct tccgggcgtc gatatcaccc aaaacggcgg ttcaggtcag ctctcatcta
     5881 tttttattcg cggtacaaat gccagtcatg tgctggtgtt aattgatggc gtccgcctga
     5941 atctggcggg ggtgagtggt tctgccgacc ttagccagtt ccctattgcg cttgtccagc
     6001 gtattgaata tatccgtgga ccgcgctccg ctgtttatgg ttccgatgca ataggcgggg
     6061 tggtgaatat catcacgacg cgcgatgaac ccggaacgga aatttcagca gggtggggaa
     6121 gcaatagtta tcagaactat gatgtctcta cgcagcaaca actgggggat aaaacacggg
     6181 taacgctgtt gggcgattat gcccatactc atggttatga tgttgttgcc tatggtaata
     6241 ccggaacgca agcgcagaca gataacgatg gttttttaag taaaacgctt tatggggcgc
     6301 tggagcataa ctttactgat gcctggagcg gctttgttcg cggctatggc tatgataacc
     6361 gtaccaatta tgacgcgtat tattctcccg gttcaccgtt gctcgatacc cgtaaactct
     6421 atagccaaag ttgggacgcc gggctgcgct ataacggcga actgattaaa tcacaactca
     6481 ttaccagcta tagccatagc aaagattaca actacgatcc ccattatggt cgttatgatt
     6541 cgtcggcgac gctcgatgag atgaagcaat acaccgtcca gtgggcaaac aatgtcatcg
     6601 ttggtcacgg tagtattggt gcgggtgtcg actggcagaa acagactacg acgccgggta
     6661 caggttatgt tgaggatgga tatgatcaac gtaataccgg catctatctg accgggctgc
     6721 aacaagtcgg cgattttacc tttgaaggcg cagcacgcag cgacgataac tcacagtttg
     6781 gtcgtcatgg aacctggcaa accagcgccg gttgggaatt catcgaaggt tatcgcttca
     6841 ttgcttccta cgggacatct tataaggcac taaatctggg gcaactgtat ggcttctacg
     6901 gaaatccgaa tctggacccg gagaaaagca aacagtggga aggcgcgttt gaaggcttaa
     6961 ccgctggggt gaactggcgt atttccggat atcgtaacga tgtcagtgac ttgatcgatt
     7021 atgatgatca caccctgaaa tattacaacg aagggaaagc gcggattaag ggtgtcgagg
     7081 cgacagccaa ttttgatacc ggaccactga cgcatactgt gagttatgat tatgtcgatg
     7141 cgcgcaatgc gattaccgac acgccgttgt tacgccgtgc taaacagcag gtgaaatacc
     7201 agctcgactg gcagttgtat gacttcgact ggggtattac ttatcagtat ttaggcactc
     7261 gctatgataa ggattactca tcttatcctt atcaaaccgt taaaatgggc ggtgtgagct
     7321 tgtgggatct tgcggttgcg tatccggtca cctctcacct gacagttcgt ggtaaaatag
     7381 ccaacctgtt cgacaaagat tatgagacag tctatggcta ccaaactgca ggacgggaat
     7441 acaccttgtc tggcagctac accttctgaa ccacgtccca ccgtgctggt gtttgactcc
     7501 ggcgtcggtg ggttgtcggt ctatgacgag atccggcatc tcttaccgga tctccattac
     7561 atttatgctt tcgataacgt cgctttcccg tatggcgaaa aaagcgaagc gtttattgtt
     7621 gagcgagtgg tggcaattgt caccgcggtg caagaacgtt atccccttgc gctggctgtg
     7681 gtcgcttgca acaccgccag taccgtttca cttcctgcat tacgcgaaaa gttcgacttc
     7741 ccggttgttg gtgtcgtgcc ggcgattaaa cctgctgcac gtctgacggc aaatggcatt
     7801 gtcggattac tggcaacccg cggaacagtt aaacgttctt atactcatga gctgatcgcg
     7861 cgtttcgcta atgaatgcca gatagaaatg ctgggctcgg cagagatggt tgagttggct
     7921 gaagcgaagc tgcatggcga agatgtttct ctggatgcac taaaacgtat cctacgcccg
     7981 tggttaagaa tgaaagagcc gccagatacc gttgtattgg gttgcaccca tttccctcta
     8041 ctacaagaag aactgttaca agtgctgcca gagggaaccc ggctggtgga ttctggcgca
     8101 gcgattgctc gccgaacggc ctggttgtta gaacatgaag ccccggatgc aaaatctgcc
     8161 gatgcgaata ttgccttttg tatggcaatg acgccagaag ctgaacaatt attgcccgtt
     8221 ttacagcgtt acggcttcga aacgctcgaa aaactggcag ttttaggctg atttggttga
     8281 atgttgcgcg gtcggaaaat tattttaaat ttcctcttgt caggccggaa taactcccta
     8341 taatgcgcca ccactgacac ggaacaacgg caaacacgcc gacgggtcag cggggttctc
     8401 ctgagaattc cggcagagaa agcaaaaata aatgcttgac tctgtagcgg gaaagcgtat
     8461 tatgtacacc ccgcgc
//
LOCUS       DAJTUP010000143         8467 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTUP010000143 DAJTUP010000000
VERSION     DAJTUP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.145"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            15..368
                     /locus_tag="OO468_003591"
     CDS             15..368
                     /locus_tag="OO468_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR6260563.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(405..2483)
                     /gene="flhA"
                     /locus_tag="OO468_003592"
     CDS             complement(405..2483)
                     /gene="flhA"
                     /locus_tag="OO468_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR6260564.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(2557..3567)
                     /gene="hypE"
                     /locus_tag="OO468_003593"
     CDS             complement(2557..3567)
                     /gene="hypE"
                     /locus_tag="OO468_003593"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR6260565.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(3564..4685)
                     /gene="hypD"
                     /locus_tag="OO468_003594"
     CDS             complement(3564..4685)
                     /gene="hypD"
                     /locus_tag="OO468_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR6260566.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(4685..4957)
                     /gene="hypC"
                     /locus_tag="OO468_003595"
     CDS             complement(4685..4957)
                     /gene="hypC"
                     /locus_tag="OO468_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR6260567.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(4948..5820)
                     /gene="hypB"
                     /locus_tag="OO468_003596"
     CDS             complement(4948..5820)
                     /gene="hypB"
                     /locus_tag="OO468_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR6260568.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(5824..6174)
                     /gene="hypA"
                     /locus_tag="OO468_003597"
     CDS             complement(5824..6174)
                     /gene="hypA"
                     /locus_tag="OO468_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR6260569.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            6386..6847
                     /gene="hycA"
                     /locus_tag="OO468_003598"
     CDS             6386..6847
                     /gene="hycA"
                     /locus_tag="OO468_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR6260570.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            6972..7583
                     /gene="hycB"
                     /locus_tag="OO468_003599"
     CDS             6972..7583
                     /gene="hycB"
                     /locus_tag="OO468_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR6260571.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            7620..>8467
                     /locus_tag="OO468_003600"
     CDS             7620..>8467
                     /locus_tag="OO468_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="catalyzes the oxidation of formate to carbon
                     dioxide and molecular hydrogen; formate hydrogenlyase
                     comprises of a formate dehydrogenase, unidentified
                     electron carriers and a hydrogenase (subunit 3); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR6260572.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIG"
BASE COUNT         1890 a         2305 c         2285 g         1987 t
ORIGIN      
        1 gaggtggagg cagcatgtcc ggcaagcgta tctctcgtga aaaactgacg attaaaaaaa
       61 tgatcgatct ttatcaagcg aaatgcccgc aggcgtcagc ggagccggag cattacgagg
      121 cgttgtttgt ttacgcgcaa aagcggctgg ataaatgtgt gttcggcgaa gagaaaccag
      181 cctgtaagca gtgtccggtg cactgttacc agcctgcaaa gcgtgaagag atgaaacaga
      241 ttatgcgttg ggccgggccg agaatgctgt ggcgtcatcc gatcttaacc gtgcgtcatc
      301 tcatcgatga caagcgtccg gtgccagaac tgccggaaaa atatcgtccg aaaaaacctc
      361 gtgaatagta tgccggacag gcagatctgt ccggcaattt gcagttaaat caatgccgat
      421 ttatcaattc ccagccgctt catccgtgac agcagggtcg tgcgtttcag ccccagacgt
      481 tgcgcagcgc ctttaggccc ggcaaccacg ctgttggttt ctttcagcac gcgcacaatc
      541 aactgatatt catcttcgcc ctcctgggcg acaaccgttg cggcaggcgg cgtttcaggc
      601 tccggtaaag caatatctgg caatgacagc tgcagcacgt tgccgcgtgt tagcaatact
      661 gcgcgctcaa tgacgttttc cagttcgcgt acgttacccg gccactccat gttgctcaag
      721 gtgcgcagcg tctcggcagg aatgctgtcg atattgcgcc ccagacgacg ggcaattttg
      781 aaggtaaagg ctttcgccag cagcggaata tcttccggac gctcgcgtag tggcggcagg
      841 tgaatcggga atacgttcag gcggtaatag agatcgctac ggaactctcg gtcggcgacc
      901 atttttttca gatcgcggtt agtcgcggca attaaacgta cgtccgtctg aatgattttg
      961 ttgctgccga gacgttcaaa ctcctgttcc tgcaatacac gcagcaactt cggctgtaac
     1021 tccagtggca tatcgcccac ttcgtcgagg aacagagagc ttttatccgc cagttcaaaa
     1081 cgaccaatgc gctgggcgtt ggcaccggta aaagccccac gctcatggcc aaacagatcg
     1141 ctttccagca atccggcagg catcgccgcg cagttcattt tgaccatgcg gcgattatta
     1201 cgcccactga gattatggat cgcacgggca atcagctctt tacccgagcc agtttcaccg
     1261 aggatcagca cggtactgtc actttgcgcc accatttcaa cttgtttaag cacgctgtac
     1321 atggcttcgc tgcggccaat aatctcgcca aattcactat caacattgtt gagctgctcg
     1381 gtcagggcga ggttttcatc aaccagccgt tctttcagac gatggatttc ctgataggcg
     1441 agggcgttat cgacagcgat tgccacacgt tcggcaatct ggcgcagcaa attcagattg
     1501 gtagtggtaa acactttctc ttcgcattgc gccagtttca gcacgcccag catggtgtcg
     1561 ccagacatca gcggtaacag gcacaaggtt tgaatctggt tgccccaggt gtcgaacaac
     1621 atgcgttcat agggggctaa atcgtcccgc tcgtagagat tgatcagcag catctcttta
     1681 cttttgaaca cgcgttcggt gagggttccg gcttcatcga cttcgctctg ttcgtgggcg
     1741 ggatgttgtt tatcaagata gtgagtggag tagatgttga gtttgttttt acggtggctg
     1801 cgtaagacaa tgctgatatc gtcaatgtcg aaatagtaat ggatttcttt ggcgacttcg
     1861 ctgaccagtt cgtccatatc caggcgggaa agcaccgcgt tggtaatggc aaccaggatg
     1921 cggaagttat cgcgctcccg gcataacaac tcatagtcga cattgttgac gacgcggctc
     1981 tggatttgtt cggtgacgac agaaacgatc tgcgtaaatg tttgcagacg attgaactct
     2041 ttttcgctcc agggacgctc gtcataacga ataaattcac agccaccaaa aatatgccct
     2101 cccgccgcca gtggcatcag gcaatagtga ccaaatttgg ggtatagccc acctgcggcc
     2161 agctgcggcc aggtttcaca aaattcttcg taactgcaat gcagcgtatc agggcgcgac
     2221 aaaatgctgc gtaccggacc gtgtgccaga acagtttcgt cttcatattt aatgggggtg
     2281 tctttttcac gcgacgcgta ataagacgca cgttgagtct gcgcttgcca caacacaata
     2341 gccgcgttgt cggcgagcgc agaacgcttt accagttgcg aaagagcctc acacagcgag
     2401 gccagatcgg gctgctgcaa tagtgtccga gtgatgtcga acaacccttg ctgtccgaga
     2461 tcactcatcg gtgtatatga cattttgttc ttccaggaaa gcaccgcaaa gcggtgcaaa
     2521 aggtcattga ctaactatag gagatttaga attttattag catatacgcg gaagcggttc
     2581 ggcgtgtggt aaatcgaggg ttcgtttcac gccatacaga ccggcaagac gaacaccttt
     2641 acgttccacc acttcaccaa tcagcgccgc gtctttcccc agtggatggg aatgtaacgc
     2701 tgccagcact tgctctgccg cgttgcgttc aacggcgatc accagtttgc cttcgttggc
     2761 aaagttcagg gcgtccagtc ccagcaattc gcaaacgccg cgcacggcag gtttaaccgg
     2821 cagcgctgat tcagaaattt caataccaca accgcaggct gccgcgaact catgaaccac
     2881 cgcgtttaca ccaccacggg tggcatcacg cagcgctttc acgccgggaa tgtcacgcag
     2941 cgtctgaata agcggcgtca gcaccgcgca gtcgctgacc agttcgccat ccagccccag
     3001 ctgctcacgc aggttaagga tagtcgcccc gtggtcgccg agtgtaccgc taaccagcaa
     3061 tacatcgcct gcggttagcg tctgtgcgcc ccagtgaata ttcgccggaa ttgcgcccat
     3121 gccagcggtg ttgataaaca gtttatctgc cgcgccgcgc tgcaccactt tagtatcgcc
     3181 agtaacgatg gcaatgcctg ccgtgcgggc ggtttctgcc atgctggtca ctacggcttt
     3241 cagtgtctcc atcggcaatc cttcttcgag gataaagcca caggagagat agcgcggaat
     3301 agcgccactg cccgcaacgt cattcgcggt gccgcaaatc gccagcttgc cgatattacc
     3361 gccaggaaag aacagcgggt caatgacgta actgtcggtg gagaacgcca gacggtcgcc
     3421 ttccgctacc agctgcgcca gatcaagacg tgcctgatct tcctgctctg ccagccacgg
     3481 gttggcaaag gcttccataa acaggctgtt gattaattgc tgcatcgcct ggccgccgct
     3541 gccgtgggcg agttggatat tattcacgct tcactctcct gctgacgata ctgataccac
     3601 gcggcgcacg ctccttcgga ggagaccatc agtgcaccaa atgcggtttg aggattacag
     3661 gtgttaccaa acagcgggca ttgatgcggc ttacatttgc ccgttaatac ctcaccacaa
     3721 cgcgcgcgcg ggtcatcgca gacctgctgc ggtgccgggc ggaaatgtgc ttcggcatcg
     3781 aatcgttgat aatccggcgt caggtgcacg ccagaagatt caatcacgcc taagccgcgc
     3841 cattcgctgt cgccgttgac acagaacaca tcggcaatcg cctgttgcgc cagtaggtta
     3901 ccggcatccg gtaccacccg acgatactga ttctctacct tgctgtgggc cgctattttc
     3961 tgctccacca gcatgaccac gccttgcagt agatcaaggg gttcgaaacc agcgaccacc
     4021 agcggacgat gaaaatcgct ggcgataaaa ttataggcat cagtgccgat aaccatacta
     4081 acgtggcccg gcgcgaggaa cgcgtcgata ccgttatccg gctgttccag caaactgcgc
     4141 agcgtcggga taagcgtaat gtgctggcag aagaagtaaa aattctgcac atcacgcgct
     4201 ttcgcctgtt gcagagtgat ggcggtggtc ggcatggtgg tttcaaaacc taagccgaag
     4261 aacaccactt tgcgggttgg attctcctgc gccagtttca acgcatccat cggcgagtaa
     4321 acgatgcgca catcggcacc gcgtgctttt gcctgcaaca gcgatccctg tttccctggt
     4381 acgcgcatgg cgtcgccaaa ggtacagaag atgacttccg gatggctggc aatctccacg
     4441 caggtgtcga ttctacccat cggcagtacg cacaccgggc aacccggacc gtggataaac
     4501 tcaacgtttt caggcagtaa ctggtcgagg ccgaatttaa aaatggcgtg ggtatgaccg
     4561 ccacacactt ccataatccg cagagggcgt tcggcggtgt aagagagatg tgaagcacgt
     4621 tcgcgcagat gctcaattaa ctgcatcacc tgttccggcg cgcgatattc atcaacaaaa
     4681 cgcattattt ttcctcgcca tacaacagcg cgccgacatc cggctcaacg tcaaacatgt
     4741 tttgtaaggc gtcgagagtg tcgcgtgctt cggcttcatt aattacgctc atggcaaagc
     4801 caacgtgaac cagtacccac tggcccacgc gcggctgacc gttttcatcg cagctgccga
     4861 ctaacgttaa atcgacatcg cgctgaatgc cgcagacgtc gactttcgcc tggttgccgt
     4921 caatggtgcg gatctggccg ggaacgccta tgcacatcgc tgtgtctcca gccagttcag
     4981 ccactggtcc atcccttcgc cgctggtggc ggaaataagg atgatttcaa tttctggatt
     5041 gacttcgcgg gcgcaggcga tgcacttctc aacgtcaaag ttgagatacg gcaacaggtc
     5101 aactttgttg agcagcatca gcgaggcggc ggcaaacata tgcggatatt tcagcggttt
     5161 gtcttcacct tcggtaacgg aaagcaccgc tactttgtgt ttttcaccga gatcgaagct
     5221 ggccgggcat acgaggttgc caacgttttc gataaacaga ataccgttat cgtccagtgg
     5281 cagacgcggt gcggcgtcgg caatcatctg tgcgtcaaga tggcagcctt taccggtgtt
     5341 cacctgaatc gctggtgtac cggtagcgcg aatgcgtgcg gcatcgttca cggtttgctg
     5401 gtcgccttca ataaccgcgc acggaacgct gtctttcagg cgcattaagg tttccgtcag
     5461 cagggtggtt ttaccggaac cagggctgga aaccaggttg agcaccagtt gcttgcgggc
     5521 agcaaagcgc gcgcggttgc gttcagccag acggttgttt ttgtccagca cgtcaatttc
     5581 gacttccagc atccgacgct ggctcatgcc cggtgtgtga gtgcccgctt cgccatgacc
     5641 gtaatgcagg tcgccttctt cagtctggct gggggtaaat tcaggcgctt taatgccggt
     5701 gattttcatc tgcgggcgtg ccgccggggc aaatggcgcg ctacgaaacg cggaatgagg
     5761 gttatgttca tcaccctcga tatacaggtt gccttcaccg caaccgcatg ttgtacacat
     5821 cgctcactcc tggtctattt ctatccgccg aatctgtaaa ccgtcgtctg ccacaatctg
     5881 tagcatgtca ccatgacact gtggacagcg gcggacgcgc tgggtcagta gcgtcacata
     5941 ctgttggcat gtttcacacc agcattcggc ctcttgttct tcgaggtgca gtttacaacc
     6001 ttccgccacg ctgccgcggc aaaccagatc aaaacaaaag gcaagagagc tggtttcgac
     6061 acaagaaaat gcgccaattt tgagccagac cccagttacg cgttttgcgc cgtgttttgc
     6121 ggcctgctgt tcgatcaatt ccagtgcccg ttggcagagg gttatttcgt gcatatcgcc
     6181 tcccattaac tattgccagc tacaagcaat aattgcgcca gtgttgatta tccctgcggt
     6241 gaataatgtc gatgatgtcg aaatgacacg tcgacacggc gacgaaattc atctttagct
     6301 taaaaatctc tttaataaca ataaattaaa agttggcaca aaaaatgctt aaagctggca
     6361 tctctgttaa acgagtaacc tgacaatgac tatttgggaa ataagcgaga aagccgatta
     6421 catcgcacag cggcatcgtc gcctacagga ccagtggcac atctactgca attcgctggt
     6481 tcaggggatc acgttatcga aagcgcgcct gcatcacgcc atgagctgcg cgccggacaa
     6541 agaactctgt ttcgtccttt ttgaacattt tcgtatttac gtcaccctgg cggatggctt
     6601 taacagccac accatcgagt attacgtcga aacaaaagat ggcgaagaca aacagcggat
     6661 tgcgcaggcg caactgagca ttgacggcat gattgatggc aaggtcaaca tccgcgatcg
     6721 cgaacaggtt ctggaacact atctcgaaaa aatcgctggc gtttacgaca gcttatacac
     6781 cgccattgaa aacaatgtgc cggtgaattt aagccaactg gtaaagggac aaagcccggc
     6841 agcatgagct gaggctttgc ccgttttgca ggcgttacgc ctgtttgggg atgggcgtgt
     6901 cgatgagtgt cgaaaatgac atttcatcgg catgttttcg tcaaaaatga caatcacctg
     6961 aggaatgcct ggtgaatcgt tttgtaattg ccgactccac gctctgtatc ggctgccaca
     7021 cctgtgaggc cgcctgttca gagacgcatc gccagcacgg cctgcaatca atgccgcgcc
     7081 tgagagtgat gctcaatgaa aaagaatctg cgccgcagct ctgtcaccac tgtgaagatg
     7141 caccctgcgc ggtggtctgc ccggttaacg ccatcacccg cgttgatggg gccgtgcagt
     7201 tgaatgaaag tctgtgcgta agctgcaaac tgtgcggcat cgcctgcccg tttggcgcaa
     7261 ttgaattttc cggcagccgt ccgctggata ttccggcaaa cgccaatacc ccgaaagcgc
     7321 caccggcacc gcctgctccg gcgcgtgtca gcacattgct tgactgggtg ccaggtattc
     7381 gcgcgatcgc cgtcaaatgt gacctttgta gctttgatga acaaggtccg gcctgtgtgc
     7441 ggatgtgccc gactaaagcc ctgcatctgg tggataacac cgatatcgcc cgcgtcagca
     7501 aacgtaagcg tgagctgacc tttaacacgg actttggcga tctcaccttg tttcagcagg
     7561 ctcaaagtgg agaggctaaa tgagcgcaat ttccctgatc aatagcggcg tggcatggtt
     7621 tgtcgccgcc gctgttctgg catttctctt ttcaaaaagc gctaagtggc tggatagccg
     7681 gaattggcgg cgcggttggt agtctgtata cggcagccgc gggcttcact gtactgactg
     7741 gcgcggttgg cgtgagcggt gcgctgtcgc tggtaagcta cgatgtgcaa atctccccgc
     7801 ttaacgcgat ttggctgatt accctcggcc tttgcggtct gtttgtcagc ctctataaca
     7861 ttgactggca tcgccacgcg caggtgaagt gcaacggctt gcagatcaat atgttgatgg
     7921 ctgccgccgt ctgcgccgtc atcgccagca acctcggcat gttcgtggta atggccgaag
     7981 tcatggcctt gtgtgcggtg ttcctcacca gcaacagcaa agagggcaaa ctgtggtttg
     8041 cgctgaggcg tcttggcact ctgctgctgg tgattgcttg ctggctgctg tggcagcgtt
     8101 atggcacgct ggatctgcgc ctgctggata tgcgtatgca acagctgccg ctcggttccg
     8161 atatctggct gctcggtgtg attggctttg gcctgctggc tgggattatt ccgctgcacg
     8221 gctgggtgcc gcaggcacat gctaacgcct ctgcgccagc tgccgcgttg ttttctacgg
     8281 tagtcatgaa aattggcctg ctgggcatgt taaccctgtc actgctgggc ggtaatgcac
     8341 cgctgtggtg ggggatcgcg ctgctggtgc tcggcatgat caccgcgttc gtcggtggtc
     8401 tgtatgcgtt gatggaacac aacatccagc gcctgctggc ttaccacacc ctggaaaata
     8461 tcggcat
//
LOCUS       DAJTUP010000144         8443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTUP010000144 DAJTUP010000000
VERSION     DAJTUP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.146"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..507
                     /locus_tag="OO468_003601"
     CDS             <1..507
                     /locus_tag="OO468_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6260573.1"
                     /translation="YLGNAFALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQEN
                     RLSVDTTQLPDNVDLEQTTQFVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGA
                     LASNDDTGQQSIVDEGGILYLSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSV
                     LQGTAQCH"
     gene            518..1609
                     /locus_tag="OO468_003602"
     CDS             518..1609
                     /locus_tag="OO468_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6260574.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1652..2512)
                     /gene="rsmI"
                     /locus_tag="OO468_003603"
     CDS             complement(1652..2512)
                     /gene="rsmI"
                     /locus_tag="OO468_003603"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR6260575.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2577..4613
                     /gene="lpoA"
                     /locus_tag="OO468_003604"
     CDS             2577..4613
                     /gene="lpoA"
                     /locus_tag="OO468_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR6260576.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4571..4966
                     /locus_tag="OO468_003605"
     CDS             4571..4966
                     /locus_tag="OO468_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR6260577.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4986..5576
                     /gene="diaA"
                     /locus_tag="OO468_003606"
     CDS             4986..5576
                     /gene="diaA"
                     /locus_tag="OO468_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR6260578.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5586..6161
                     /gene="yraP"
                     /locus_tag="OO468_003607"
     CDS             5586..6161
                     /gene="yraP"
                     /locus_tag="OO468_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR6260579.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6275..7315)
                     /locus_tag="OO468_003608"
     CDS             complement(6275..7315)
                     /locus_tag="OO468_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR6260580.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7388..8023)
                     /locus_tag="OO468_003609"
     CDS             complement(7388..8023)
                     /locus_tag="OO468_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR6260581.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8151..>8443
                     /gene="yhbO"
                     /locus_tag="OO468_003610"
     CDS             8151..>8443
                     /gene="yhbO"
                     /locus_tag="OO468_003610"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR6260582.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSG"
BASE COUNT         2128 a         2270 c         2160 g         1885 t
ORIGIN      
        1 tatctgggca acgcttttgc gcttatcgat gctaacgggg cttctggcgt gaggatacaa
       61 aactatccgg ggattgctac tgatcccttt ggctatgcag tggttcctta tctcacgact
      121 tatcaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt cgatcttgaa
      181 caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg tttcaacgcc
      241 aatatcggtt atcgcgtact tgttacagtc agcgatcgca acggtaaacc gttgcccttt
      301 ggcgctcttg ccagcaacga tgatacgggg caacaaagta tcgtcgatga gggcggcata
      361 ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg gggaaatcag
      421 gcagatcaac aatgtcagtt tgcttttagt acaccggatt cagaaccaac aacctctgta
      481 ttacaaggca cagcgcagtg ccattaagga taaaaaaatg aaaagagcgc ctcttataac
      541 aggacttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagggt gtggagccga
      601 cagcactagc ggtgcgacaa attacagcag tgtggttgat gatgttacgg tgaaccagac
      661 agataacgtg acaggacggg agtttacctc tgcaacgcta agtagcacta actggcaata
      721 cgcctgttcc tgctctgcgg gtaaggcagt taaacttgtc tatatggtca gccccgtact
      781 taccaccact ggacatcaga caggatatta caaactcaat gacagcctgg atattaaaac
      841 cacattacag gcaaacgaca ttccaggact cacaaccgac caggttgtct ctgttaacac
      901 ccgattcaca cagataaaaa gcagcaccgt atattctgct gcaacccaaa cgggtgtttg
      961 ccagggtgac acgtctcgtt atggacccgt taatattggt gcaaatacca cctttaccct
     1021 gtatgtcacc aagccatttc tcggctcgat gaccattccg aaaacggata ttgccgtcat
     1081 taaaggcgcg tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc atgatttagt
     1141 caagttatcg attcagggaa atctcaccgc cccacagtcg tgcaaaatta atcagggcga
     1201 tgttattaaa gttaattttg gattcatcaa tggtcagaag tttaccaccc gcaatgccat
     1261 gccagacggt tttactccag tagactttga tatcacttat gactgtggtg atacttcaaa
     1321 gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag accagtacaa
     1381 cctggtcgcc aggcgaagaa gttcagacaa tgtgcccgat gtcggtattc gtattgaaaa
     1441 tctcggcggc ggagttgcaa atattccttt tcagaacggt atccttcccg ttgatccttc
     1501 cgggcatggc accgtcaata tgcgcgcctg gccagttaat ctggtcggtg gtgagctgga
     1561 aacaggaaaa tttcagggca cagccaccat taccgtcatg gtgcggtaaa agaagacgtt
     1621 gcgttttgat taacgcaacg cttcggcact gttacccctg ctgctccagc gcatacttat
     1681 acagcgcatt tttcttcacg ccatgaattt ctgcggccag cgccgccgct tttttcagcg
     1741 gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag tcttcttcct
     1801 gtgctttatg accttcgaca atcagcacca tttcgccttt gcgacggttt tcatcttcct
     1861 ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag gttttggtca
     1921 gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata tcttccaggc
     1981 tatctaacag acggtgggta gattcataaa aaatcagcgt gcgcggctcc gcttcaatgg
     2041 cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttcg taacagaaac
     2101 ggtcagatgg taaacccgcg gcgcttaacg cagtgatagc agcacacggt ccgggtagcg
     2161 gcaccacacg aatccccgct tcacggcagg tacgcaccag atggtagcca ggatcgttaa
     2221 ttagcggcgt tccggcatcg gaaaccagcg caatgttttg cccctcttgc agctttgcca
     2281 gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac cgggcattaa
     2341 tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca atcagatcaa
     2401 cggcctgtaa tacctctaac gtacgctggg tgatatccgc cagattgccg attggcgtcg
     2461 gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc attgtgtcgt
     2521 ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa tatcactgga tacattatgg
     2581 taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt ctggcagccc
     2641 tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat atgcagggca
     2701 cggcgcaggc tgattctgcc ttttatcttc agcagatgca gcaaagctct gatgatacca
     2761 ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa accgggcagg
     2821 cggttgagtt gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt cgcgagaaaa
     2881 cactgctggc ggcagagatt aaactggcgc agaaagattt tgctggcgcg caaaacttgc
     2941 tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcgcgttac tggcaggcaa
     3001 aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta attgctcagg
     3061 aaccgctgct tggcgcgaaa gaaaaacagc agaatattga tgccacctgg caggcgctct
     3121 cctccatgac tcaggaacag gcgaatacgc tggtgatcaa cgccgacgaa aatattctgc
     3181 aaggctggct ggatctgcag cgcgtctggt ttgataaccg taacgatccc gacatgatga
     3241 aagccgggat cgccgactgg cagaaacgtt atccgaacaa tccgggcgcg aaaatgctgc
     3301 caacgcagtt ggttaacgta aaagcgttta aaccagcctc gaccaacaaa atcgccctgc
     3361 tgttgccgct gaatggccag gcagcggtat ttggtcgcac tattcagcaa ggctttgaag
     3421 ccgcgaaaaa tatcggcact cagccagtgg cggctcaggt agctgccgca cctgccgcag
     3481 acgtagcaga acaacctcag ccgcaaactg cggatagcgt tgccagcccg gcacaagcct
     3541 cggttagcga tctgaccggt gatcagcctg cggcccagcc ggtgcctgta agcgccccgg
     3601 cgacaagcac cgcagcggta agcgcacccg caaatccatc cgcagagctg aaaatctacg
     3661 atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat ggcgcgagta
     3721 ttgtggttgg tccgttgctg aaaaataacg ttgaagagtt gctgaagagc aacaccccgc
     3781 tgaacgtact ggcactgaac cagccggaga atatcgaaaa ccgcgtcaat atttgttact
     3841 tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt gaccagggta
     3901 aacaagcgcc gctggtgctg atcccacgca gtgcattggg cgatcgcgta gccaatgcgt
     3961 ttgcgcaaga gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa tttggttcca
     4021 ccagcgaatt acgcgcgggt gttaacggcg gttctggtat tgctttaacg ggtagcccga
     4081 ttactcccag agcgacaacc gactccggca tgacgaccaa caatccaacg ctgcaaacca
     4141 cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtc tacattgtgg
     4201 caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac ggtagccaga
     4261 gcggtgcaac gctgtacgcc agttcccgca gcgcacaagg caccgcaggc ccggatttcc
     4321 gactggagat ggaaggcttg cagtacagcg aaatcccgat gctggcaggc ggtaatctgc
     4381 cgttaatgca gcaggcgctc agcgcggtga acaacgatta ttcgctggct cgcatgtatg
     4441 cgatgggcgt tgatgcctgg tcgttggcaa atcatttctc acaaatgcgc caggttcagg
     4501 gttttgaaat caacggtaat accggaagtc tgacggctaa cccggattgc gtgattaaca
     4561 ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt taaccaccaa
     4621 acagaccggc gatgcgtggg aagcacaagc gcgtcgctgg ctggaaggca aaggactgcg
     4681 gtttatcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa tgcgtgaagg
     4741 cctgaccacc gtttttgtcg aggtacgcta tcgccgctct gcgctttatg gcggcgcggc
     4801 agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct tgtggctcgc
     4861 gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag ccttcaccgg
     4921 gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa ggtttaagga
     4981 ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact caaattgcgg
     5041 cggcagaggc gcttccggat gccatctccc gtgcagccat gacgctggtt cagtctctgc
     5101 tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat gcacagcatt
     5161 ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct gccattgcac
     5221 taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat gatgaagtgt
     5281 atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc atttccaccc
     5341 gtggcaacag ccgcgatatt gttaaagcag ttgaagccgc cgttacgcgt gatatgacca
     5401 ttgtggcatt gaccggctat gacggcggcg aacttgcagg tttgttaggg ccacaggatg
     5461 tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat atgctgacgg
     5521 taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat gattaaggag
     5581 aatacatgaa ggcattatcg ccaatcgcag tccttatttc cgcgctgctg ttgcaaggtt
     5641 gtgttgccgc tgccgtagtg ggtaccgctg ctgtgggtac caaagccgca actgacccac
     5701 gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac agcgcattgt
     5761 cgaaagacga acagattaag aaagaaaccc gtattaatgt cacggcctat cagggcaaag
     5821 tgctgctggt tgggcagtca ccaaatgctg aactttcggc tcgcgccaaa cagattgcta
     5881 tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag ccgattggtc
     5941 tgggcgaagc atctaacgat acgtggatca ccaccaaagt gcgttcgcag ctcttaacca
     6001 gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa gtgttcctga
     6061 tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc cgggtgagcg
     6121 gcgtaaagcg ggttaccaca gcgtttacgt ttattaaata gcagcccctt gtaatgcctg
     6181 atgcgacgct tgccgcgtct tatcaggcct acaacgatgc aaaccgtagg tcggataagg
     6241 cgtttacgcc gcatccgaca tgttttccct caaatcagaa caacagcgcc agcccgccga
     6301 caatcacacc agacactgcc accatcgccc ctgtcagcca taaggctttc gccgggaacg
     6361 ctttgcgcag cataatcagt gacggcaaac tcactgccgg gagcgtcatc aacaatgcca
     6421 gcgccggagc ggttcccata cctgccagca tcatcgtttg tacaatcgga atttctgctg
     6481 ccgtgggaat gacaaacaag catcctgcta ccgccatcgc caccacccac atcaggctgt
     6541 tatcgacagc accatcggca tgggggaata accagacgcg tgcggcaccc aacaccagta
     6601 ctgcaaggat gtaaaccggg atcgtactcc agaaaagtgt ccatagcgcc ctgccccagc
     6661 ggctaaaaaa accgctctgt gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg
     6721 gtgtttcacg cacccatttt tgcaccagcg tcgcaatcag caacaccatc accagcccgg
     6781 ccaccagacg aatcgccgca aaaccccagc cgaggacaaa ccccataaac accagcgtcg
     6841 ccgggtttaa caccggattg cccatccaga atgccagcgc accgcccatc gatacctgtt
     6901 ggcgacgcat tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcagcg
     6961 aaaacagcgt tcccagcagc gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc
     7021 agtcacgcgg gatcagcacc tgaatcaacg aaccgaggat cacccccagc accgccgctt
     7081 tccatactgc gaggaaatag atcatcgcgt aatccaacgc cgcttgccac ggattagcat
     7141 ccgcctgcgc aaggatagat ttaccgatac tgtgggtttc ggctgcagta aacgctttgc
     7201 cgtagtaagg ttgccatttc acataccaga gaccggcaat gacaacgaga aagaaaagcg
     7261 cgggtttcca ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat
     7321 tccccaggaa taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg
     7381 ggaataatta ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acgccttcat
     7441 gctcaggccg catcgcctct gccagcatga cccgcgccac gtctctcgca tcaatggatt
     7501 tccagttacc tggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt
     7561 tgctacgatc gcccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg
     7621 caattaatgc ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcat
     7681 tggcacccat agcactgacc accagcatat gctgcgcgcc cagtcgccgc ccggttaatg
     7741 cggtatccac taccagcgtg taatcggcat gaataaacgc ctctttgctc cccgcttctc
     7801 gccgcgtggt gccgagacaa caaaacacaa tgtcgatggg ttcggtgacc tgcgccaacg
     7861 cgtcactcag ttgcggatca tggggattaa acaccccggg catatcgccc aacggacgtc
     7921 gcgtcggcgc agcaatggcg ttaactttcg gttcattaat caacatccgc agcaggtgac
     7981 cgcccaccag ccccgttgcg cctgtaatca gtacctgact catcttcgtt cctttacaga
     8041 attgtctgcc ttgcgctccc cggctcatgc accagtctta atagaccatg aggtaattat
     8101 ccccgattgt ggaaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga
     8161 aaattgccgt tttaatcact gatgaatttg aagattcaga atttacttca cccgcagacg
     8221 agttccgtaa agccggacac gaagtgatta ctattgaaaa acaagcgggt aaaacggtga
     8281 aaggcaaaaa aggagaagcc agcgtgacca tcgataaatc catcgatgaa gtgacgccag
     8341 cggagtttga tgccctgttg ctaccgggtg gtcattcacc ggattatctg cgtggcgaca
     8401 accgttttgt cacctttacc cgtgattttg tgaatagcgg caa
//
LOCUS       DAJTUP010000145         8418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTUP010000145 DAJTUP010000000
VERSION     DAJTUP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.147"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..722
                     /locus_tag="OO468_003611"
     CDS             <1..722
                     /locus_tag="OO468_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260583.1"
                     /translation="CKELPQSVEYVPLACEAHSHRSVALAKTVAAPIMATGVGLALMY
                     ATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSPAFDKWNELVDKLNHER
                     NTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISIVLGNSMLQTLLEKRLA
                     DSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNKFNVGLQGNINTDVLQD
                     SFFKNVDFIHEYNSYTVGMGV"
     gene            1401..1619
                     /locus_tag="OO468_003612"
     CDS             1401..1619
                     /locus_tag="OO468_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR6260584.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            1837..2079
                     /locus_tag="OO468_003613"
     CDS             1837..2079
                     /locus_tag="OO468_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260585.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2409..3338)
                     /gene="fdhE"
                     /locus_tag="OO468_003614"
     CDS             complement(2409..3338)
                     /gene="fdhE"
                     /locus_tag="OO468_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR6260586.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3335..3970)
                     /gene="fdoI"
                     /locus_tag="OO468_003615"
     CDS             complement(3335..3970)
                     /gene="fdoI"
                     /locus_tag="OO468_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR6260587.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(3967..4869)
                     /gene="fdoH"
                     /locus_tag="OO468_003616"
     CDS             complement(3967..4869)
                     /gene="fdoH"
                     /locus_tag="OO468_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR6260588.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(4882..7932)
                     /gene="fdnG"
                     /locus_tag="OO468_003617"
     CDS             complement(4882..7932)
                     /gene="fdnG"
                     /locus_tag="OO468_003617"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7345..7347),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR6260589.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8126..>8418
                     /locus_tag="OO468_003618"
     CDS             8126..>8418
                     /locus_tag="OO468_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6260590.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQ"
BASE COUNT         2035 a         2111 c         2171 g         2101 t
ORIGIN      
        1 catgtaagga attaccgcag tcagtggaat atgttccttt agcctgtgaa gcgcatagcc
       61 atcgcagcgt cgcccttgcc aaaaccgtgg ctgcgcccat catggcgaca ggtgtgggct
      121 tggcattaat gtatgcaaca tcatcaggac cgtattatgc gcaaagtctt tctggttcgc
      181 cggatatcga gatgataaaa aatcgcatgg ctgaattgtt taaccagtcg gatatttctg
      241 tgcgcaacaa atatagcccg gcttttgata aatggaatga actggtagat aaattaaatc
      301 acgaaagaaa tacagttccg tttgcgtgtt tgaccaccat tttagcgacg gcgataaatg
      361 aaactcttga gggggatacg aatgttgccg ttgtgatggg gtgtaaatct gcgaaagatc
      421 gcacgatatc cattgtcctt ggcaactcga tgttgcaaac actccttgaa aaacgcctcg
      481 ccgatagtgg agaaatagaa aatctttttg atcaacaggg atattttagt tgcgattcat
      541 taacacccga agaattaatg atgttaaaag acctttttga tattcgagtt cttcatgtct
      601 ctaacaaatt caatgttgga ttgcaaggta atatcaacac tgatgtttta caagattcgt
      661 tttttaaaaa tgtcgatttc attcatgaat ataactcgta taccgtaggg atgggagtct
      721 gacgagtaaa actcattagg attatcagaa ttcggacaat gcattgcaaa aatgacatac
      781 aaagagagat gtgtgcaggt acttagccgc gaggggcatg gcgaagccat acagaaagta
      841 ccagtattca gaactccagt gaaagcggca tagtggccgc ctgctcagga gttttgctat
      901 gacccgtaat cgtgctgaac gtcgccaccc tatgatgcga ttaaaaaaac tcatcgtcgc
      961 gctgatgatt gacgtcaatg ctatcggatg atgccggggg tgttccggaa ggaattcctt
     1021 gtttcccgaa ctgttaccaa caaaattata acgatgagca agtaaagacc ggttggtggc
     1081 agtactgacc ggtctgtgat gaaagattaa tttcacctta aatcctggtg ctgttaggat
     1141 gctgacgttt ttcggctaca ccattcagat tatgtaaata aatatccctg aatataatat
     1201 attcatagca cccataactc atcctgggcg ataactaaac catttttgag taataaatga
     1261 aacaactgca acgctcttat tatttctgga aaacgcctcg caaaaacaaa atgacatgca
     1321 aaaatccgca tttttttaaa tgcttttccg gcgtaatttt cgcacataca ttaatgagta
     1381 atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta tcagaagagg
     1441 caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct gcaattttac
     1501 gcgaagcggt tgagcaatat ctggagcggc agcaatttcc ggtgcgtaaa gcaaagggag
     1561 gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat aaggaatgaa
     1621 aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat taatacaagg
     1681 ctgttttact tgaacttata ataactgcaa ctgttacatc gtatctggaa aacgcctcgc
     1741 aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcggcaagg gtacttttct
     1801 ctccatagat tcaaaaatga tattggcgag atatttatga actctctggc aggtatagat
     1861 atgggcagaa ttttactcga tttatcgaat gaggtgatta agcaacttga tgatctcgag
     1921 gtgcagcgta atcttcctcg tgcggaccta ttaagggaag cggtagatca atacctgata
     1981 aatcaatcgc aaacagcaag aaccagtgtt cctggcatct ggcaagggtg tgaggaagat
     2041 ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat gggaaaaaat gatgtcaatc
     2101 agattgctga caacgtgcgc gttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc
     2161 tacaaagtca tgcaaattca atatattgaa gtttattggt aggcctgata agcgcagcgc
     2221 atcaggcaat catacgcttg tcatcagtga catacccata accttatcga tgtgttggag
     2281 ggaagaacga ggcaaacaga caattgcggc atgatcttga atgggtaacg cggaatacga
     2341 gagatggtgt cgtcacaccc tatgagctgt aatccgggcg acctgcgcca cccggaaaac
     2401 gtcagagatt actctccttc acccggaaac aggaacgggt taatggaact acgggcgtag
     2461 ccttcttgct ccattcgcgc gtccagtacc agagaggcga ggtcatctgc cacggcttca
     2521 actttcggtt ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc atcgcagctt
     2581 tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt gccgctctgc
     2641 tcgcagttgc tgcattttac gcgcactacg tgccattcgg tttcacacag gttgcagtgc
     2701 aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg catagagcca
     2761 cataccgggc aatattgacg ttgttcgccg tattcagcgc gggctttgcc ggggatcaga
     2821 ttggccatct gcgcccagta gagcgacagc gcagcccaga taaacggcgc tttatcgctg
     2881 ctgacggacg agaaatcaga ggcaaacagc gcgctggcca tatcttccag ctcctgagtc
     2941 gatgccttct ccagattctc aatcactgcc agcgccgggc cgctcatttc aggtttcagc
     3001 tcagcaatta gcgccatcag cagcttttgc cagtgcttat cacgcggcag aacgtgaata
     3061 tccagcgggg gcttgccctg tgcgctggct tctttaatgc gcgcggtcag atccatctcc
     3121 agcggatggt cgtacagcac cactttctgg gcgtgggcga taagcgcagc aaagcgcagg
     3181 taatcaccca gcggattatt ttctgccagc tcgcgcagac gctcggcgcg gcggttgtat
     3241 aaattcttga gccgagggaa caataacggc ggaatcatat ccgccgtacg tttctcgctc
     3301 gaacccagct catcttgcgg gattatgcga atactcattc agctttcttt tccgttgtct
     3361 tgcggacctc acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg gtaacccatc
     3421 cttccaccat ggcggtaatc gtgcctttca cccaaagggc ggcgtagata tgcaccatga
     3481 taaccacaat taacgccact gcggcaaatg aatgcagcat taacgcgaat cggatcaccg
     3541 ggattgagaa agcaggcgca aaataaggac gccagataat cacgccgctc accagcagca
     3601 gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg accgaagtta taacgcccgg
     3661 tgtcacctac ttcctcgttg acgacgatct tacgaatatt cttcgcccaa aagatatcat
     3721 cccgattgat taggttttga tgccagtaac ggaaaaacat gatgatgaac gaggcaaaca
     3781 taaccacgcc gacaaacggg tgcagaattc gcgccagctg cggtgtgccc atgatttgca
     3841 tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct caccgccgcc aggatgaagc
     3901 agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg atggtgtcac
     3961 gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt cctcatccgc
     4021 acggttcgga ccgacaccca cgtagtggaa gatactggcc gcaaaggtag ccgcaaagcc
     4081 aacagccgcg agcggtttcc agatgccttt ccagaatttc acggtttcgc tgatttccgg
     4141 gttctccggc aagccatgat acagatttgg cttgtcagca tggtgcagca cgtacatgac
     4201 gtgtgtacca ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac cgcgggtttt
     4261 cagctcagca acgcgctcgc tcgccagcgt tttcatcgac tctttcgtgc cgaagtgaat
     4321 cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc ccaaccacca cgcggtcaac
     4381 gcacagcgta catttgtaga cgcggttgtc ttccgggttg aggcgcggaa tgtcgaacgg
     4441 acagcccgca atgcaataac cgcagccaat gcactgctcg gactggaagt cgacgatacc
     4501 gttggcatac tgaataattg caccttccgc cgggcacgct ttcaggcagc ctggatcgga
     4561 acagtgcata cagccgtctt tgcggatcag ccattccagt ttgtcgttct gctccacttc
     4621 cgagaagcgc attaccgtcc acgatttggc gcttaaatca ttggggttgt cgtacacccc
     4681 aatgttattg ccgacggtat cgcgaatgtc gttccactct gaacacgcca cctgacaggc
     4741 tttacagccg atacaggtgg taacgtcgat gagtttcgcc acttcttcct ggaagtcccg
     4801 cgcctgaggc gcgggggtca gaccgttagt cgcggaacga cgaatgatat cttgagattg
     4861 ataagccata tgtcgtctcc gttacacctt ttccacattc acaaggaagg acttaaactc
     4921 cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa gtattggcaa taaagccttt
     4981 tttagctacg ccttcatagc cccagtgaat cggaataccg atggtatcga tatctttgcc
     5041 gttcgctttc agcgtgcgaa tacgtttggt caccaccgct ttggctttga tatagccacg
     5101 gttggaggag actttcacgg tatcgccctg ggcaatgcca agtttattcg ccagcgactc
     5161 cccgatttcc acaaactgct ctggctgcaa aatcgcgttc aacagcgcgt gtttggtcca
     5221 gtagtggaag tgctcggtca gacgataggt ggttccgaca tacgggaact tatcggcttt
     5281 acccaatgct tcggcgtcgt ctttaaagat acgtgcagct gggttcgaga taacgtttgg
     5341 atgcagcggg ttagttccca gcggcgtttc aaacggctcg tagtgttccg ggaacggacc
     5401 ttccgccatc ttatcgaggg caaacagacg ccccatgcct tcctgctgca tgataaacgg
     5461 cccgacgccg ctacccggag gcgctgcgct gtagtccgga atatcccagc cggtccactt
     5521 agtgccgtcc catttcagca actgacgctt cggatcccac gggttaccct gcggatctgc
     5581 ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat gcccatgccc agcccagcgt
     5641 gttaccgagg ccagacggat cggcgttatc acgacgcgcc atctggttgc cttccggcgt
     5701 ccagctaccg gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc gcaattgggc
     5761 gaacgagcta agttgttggc ctttcttgac gataaccgca ccggttgccg gatcggtaat
     5821 atcggccagc gccttaccgt tgctctccat cgctacttct tccgacttcg gctcatgcgg
     5881 aatggcgtag ttccaggtca tgttcagcac ctgatccggg ttcgcgccac cctgttcggc
     5941 atacatcttg cgcaagcgca ggaagatacc ggagaggatc tcaccgtcag tcagcgcaat
     6001 ccccggggcg tccgcacctt tccagtgcca ctgcaaccag cgacctgagt taacgattga
     6061 accgttctct tctgcgaagc aggtcgatgg cagacggaac acttcggtct ggatcttcga
     6121 cgagtcaact tcgttcagct caccgtggtt ctgccagaag ttagaggttt cagtgttcag
     6181 tgggtcgatg gtgacgagga acttcagttt cgacagacaa ccgatcactt tgtttttgtt
     6241 cgggaatgag gcaacagggt taaagccctg gcagatatag ccattgacct tgccctcttt
     6301 catcatctcg aagtattgca ggacgtcgta gcctttatcc cacttcggca accagtcaaa
     6361 gccccagcta ttttccgccg tcgctttatc accaaagaag gccttcatca tagagacgaa
     6421 gaatttcggg tagttgcccc agtagtttac ctggccttcc agcagcggtt ttggcgtgtt
     6481 ggcggtaagg taggtttgca gatcggtctg cttctcgctt ggcagcgtca tgtaacctgg
     6541 cagactctgc gacagtagcc ccaggtccgt cagcccctga atattggagt gaccgcgcag
     6601 ggcgttaacg ccgccgccgg ccatccccat attgccgagc agcagctgga tcatcgccat
     6661 cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag ccgagggcat acaggaacga
     6721 ggcagtttta tcgtgagcac tggtttctgc gatgtattcg cagactttca ggaacgcgtc
     6781 ttttggcgta ccacagatgt tttcaaccac atctggcgtg tagcgggaaa cgtgctgttt
     6841 cagcaagttc cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg cgaagccgtt
     6901 ttcgtccagt tcataagtcc aggaggattt atcgtacttg cgtttttccg cgtcgtagcc
     6961 ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg atcaggctgg cgttggtata
     7021 ggcttcggtg tattcgtggt tgaatttttc attgttcagc aggtacagca atacgcctga
     7081 caggaaagca atgtcagtac cggaacgaat aggggcatag tagtcagcta ccgccgccgt
     7141 acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt tggcttccat
     7201 cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta ccgcccatca ccacgacgag
     7261 gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc
     7321 aagacttgct accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg cgagcatacc
     7381 cagcgcgcgg gagaattttt gcgttaaata gccggtttcg ttactcgacg cggaagcaca
     7441 cagcatcccg gtggagagcc agcggttaac agtcacgcct tcggcgtttt gcgcaatgta
     7501 gttagcatcg cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct cttcccaact
     7561 gatttgctgc catttatcag aacctggcgc acggtattcc ggaaacttca gacggctttc
     7621 ggagtggatg aaatccacca ggccagcgcc tttcggacaa agtgcaccgc ggttgaccgg
     7681 gtgatccgga tcgccttcga tatggaagat agatgctttg gcgttttttg ctccgtcacc
     7741 gaggctgtac atcaacagcc cacagcctac ggaacaatag gtgcaggtat tacgggtttc
     7801 gcgggtgcgc agcagtttat actgccgggt ttccgcgagt gctacgctgg gtgcaaaacc
     7861 cagtgccgct gccgtggtgc ctgccatacc gccagcgcag atcttaaaga actgccttct
     7921 gctgacctgc atggattgct ccttgtttcg acattgtcac gtcccattta cattcgcttg
     7981 ctgcgtgtgc agggagtggg agttattttt ctttgcggaa ggggccgcaa aggtccagaa
     8041 ttggctcaat ttccctccat ctaggaaggg tttgtaacag aataccataa tgttggtgtg
     8101 tatgttctta tctggttaag agaaagtgaa aaaaacacag cgaaaagaaa tcgaaaatgt
     8161 gacaaatatc acaggtgttc gtcaaattga gttatggcgg cgtgatgatt tacaacaccc
     8221 acggttagat gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg gcatttcgca
     8281 tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt tttcgctttc
     8341 cgaggggata atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc cttcctgtaa
     8401 tggtcttgaa gtacaaat
//
LOCUS       DAJTUP010000146         8355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTUP010000146 DAJTUP010000000
VERSION     DAJTUP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.148"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..289)
                     /locus_tag="OO468_003619"
     CDS             complement(<1..289)
                     /locus_tag="OO468_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6260591.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTF"
     gene            complement(356..1081)
                     /gene="fimC"
                     /locus_tag="OO468_003620"
     CDS             complement(356..1081)
                     /gene="fimC"
                     /locus_tag="OO468_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR6260592.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1118..1657)
                     /locus_tag="OO468_003621"
     CDS             complement(1118..1657)
                     /locus_tag="OO468_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6260593.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(1722..2270)
                     /gene="fimA"
                     /locus_tag="OO468_003622"
     CDS             complement(1722..2270)
                     /gene="fimA"
                     /locus_tag="OO468_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR6260594.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(2751..3347)
                     /gene="fimE"
                     /locus_tag="OO468_003623"
     CDS             complement(2751..3347)
                     /gene="fimE"
                     /locus_tag="OO468_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR6260595.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(3825..4427)
                     /gene="fimB"
                     /locus_tag="OO468_003624"
                     /pseudo
     CDS             complement(3825..4427)
                     /gene="fimB"
                     /locus_tag="OO468_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            5881..6597
                     /gene="nanC"
                     /locus_tag="OO468_003625"
     CDS             5881..6597
                     /gene="nanC"
                     /locus_tag="OO468_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR6260596.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6617..7723
                     /gene="nanM"
                     /locus_tag="OO468_003626"
     CDS             6617..7723
                     /gene="nanM"
                     /locus_tag="OO468_003626"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR6260597.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            7788..>8355
                     /locus_tag="OO468_003627"
     CDS             7788..>8355
                     /locus_tag="OO468_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6260598.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDY"
BASE COUNT         2465 a         1804 c         1728 g         2358 t
ORIGIN      
        1 taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatcg acgcgatacg
       61 tccctggcgg taattcttgc ccattttcaa aacgcgataa atcggccaca gcctggggat
      121 catccgctaa aaagcgcaga ttaaaataga ggtcggcaga tgacaaaggt gcctgtgcgg
      181 caaaagcaca ggcgacaacg agtcggacaa aaaaaccagc caaacgatgc ttacgaatat
      241 gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt attcctccgg
      301 tttaaaatca cccggcagtc aattcttttt attcaggcga aaaattcccc ctgcgttatt
      361 ccattacgcc cgtcattttg ggggtaagtg cgccataatc atttattgtt cggtaagtaa
      421 tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc gcccattgga ggcaccaatg
      481 cattttcaag aacccgggtt ccggcattca actctgttac cgtcaggtaa tagggtgtcg
      541 ggttaatcag cgtcagagaa ttcgcgctac gacgaaatct taatttttct gcggcctgat
      601 cgggtggcaa cgctaattta gccgggcgat agtacagttt aatgcggctg ataattgcga
      661 gctgtagcat attctcagtc aattttgatt tatccattga cggaatcgct ttaacgttca
      721 tccagaataa actttcccgg tcctgtggca attggttatt tgttgcatca agaatacgta
      781 aggtattctc tttttttccc ttcatcgcaa acagaggagg cgtcacgata aaacgaccat
      841 cctttacacc atcggcattt tccacccatg attgaattaa ataggtacta ttttcatcat
      901 tatttgtcac ggcaagttgc acttgttttt gccctgccgg ataaattacg cgagtcgcac
      961 ctaaggccac tcctgcttca gcgcgtccgg caaccatcat tgccatgaac atcaggatac
     1021 ctgccagcaa gcagaatgtt atttcctgcg atttccttac attgacgttt ttattactca
     1081 ctgtcctgtt cctgttatca cattatctac tgaacaatta ctgataggtt aaagagaacc
     1141 aggcctgggc attggcgatg ccgccagtaa cccgacgccc ggtagcacga tatttggcga
     1201 tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca gtttgctgga ggacgattaa
     1261 tcggtacgag gtttccttca tcatcaaaca acgctacacc aatattggag gctatccctg
     1321 gcccctctcc cacggagagc acatccggat ttttaccatc cgcgacaccg tgaaacgcca
     1381 cacccacacg ttcactcacc accgtgctac attcccgtaa atgaataaca aaaggcaccg
     1441 gtgcgctatc ttcccctgcc gcatgaaacc ggttactgct gatttgcccc atattgaccg
     1501 tcatttgttt atcaccggct tcaatccggc aagtttccgc aataatgacg ccctgaaatt
     1561 gcatatttcc gccgggcagc gtggtattcc atttatttcc ggccagagca aacattggca
     1621 gcaacgacgc taacagaaat aatctcttcc ttttcatcgt tatttttatc gcacaagggt
     1681 gggcatccct gcccgtaatg acgtccttga acctgggtag gttattgata ctgaaccttg
     1741 aaggttgcat ccgcattagc tgcacccggg gttgcctcgc cgattgcata ataacgcgcc
     1801 tggaacggaa tggtgttggt accgttattc agggttgttt gtgcactaaa tgtcgcaccg
     1861 tccagcgtca gagcattgcc tgttctgtct aggatctgca cgccaacgtt tgttgcacta
     1921 cctgcagcag aactctgcag agccagtaca tcagtacgcg tagcatcaat tgccgtacct
     1981 aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc aatcattcag ctgaatgtta
     2041 aaaccaacgg cagagctggt tgctccagcc tgcttcaggc tagcggtacg aacctgtccc
     2101 aactgaacgg tttgatcaac agagcctgca tcaactgcgc aagcggcgtt aacaacttcc
     2161 cctttaaagt gaatggtccc accatttacc gtcgtagtat cggccagagc cgctgcggaa
     2221 ctgagggaca gagccgacag aacaacgatt gccagagttt taattttcat gctgctttcc
     2281 tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc tgtacacttt gttttgtcaa
     2341 cgagtttgac tgccaacact gcacagtttt cccccaaaag atgaaacatt tggggccatt
     2401 ttgactcata gaggaaagca tcgcgcacaa actttttcag tttatttgtt ggcttaatat
     2461 tctattatta tctttattta tagattttta tattgcatga ggtggttttt ggagagaaga
     2521 atgaggaaga tgcgtcgagc cacagaaacg ttagctttac atatagcgga ggtgatgtga
     2581 aattaattta caagagaaat aatttacata tcaaatagtt agatgctttt tgtcgttttt
     2641 taatattttt atgcttgaga aaaaatacgt aacttattta tgatatggac agtttggccc
     2701 caattgtctt gtgtttattt gttttttaac tttattatca ataagttaaa tcaaacctct
     2761 tctcttttta atttttcgtt tatgagatta tttctttccc ataatccggc aaaacgagca
     2821 gcattactgg cggtataacg cacagtatgg cgaatatttc gatgcccgag ataatcctga
     2881 attaaacggg tatctgcacc acgctccgcc agttcataac cgcaagcatg ccttaacata
     2941 tgaggatgag tctgcgttac ggttccagct tcaataccgg catcgcgaat aatgcgatag
     3001 gcctgctggc gagaaagccg actcccgcgg cgagaaataa atatagcgtc ggtccggtca
     3061 gcgcctttcc agttagcacg ttcctgggtc cagcgttcca cggcttcacg ctcatcaaaa
     3121 cgtaacgggt gaacggtaga aaatccgttc ttcagtcggc gaatatttat tcgaccttca
     3181 ttaaggtcaa ggtcctgata atgcagatca agcagttcac taatacgcat cccatgccga
     3241 tatgccaaca gaataagaca ataatctctg gctcccgttg ccccgtaaca aaccgcctgc
     3301 atcatggcct gaacttcttt accggtaaga taacgacgtt tactcacaat agtagtactc
     3361 ctaactgaga tatatttgaa tgaataccta taggaaacct caatcggtca aaattagccc
     3421 aatagataaa aagataacca cacacgaagt tatgtggcta tcagtcaatt acattaaaca
     3481 tcatacaaaa ataaaatatc actgatgtga caacatctat tttcattgat ttgattcaat
     3541 ataacaatag attacagagg attaacttcc agttacttaa aaaataaaga aaaaagtgtg
     3601 gaaacaggag aatttaagtg atgtgtaaac ttatgaatga ttttgtttat ttattttgaa
     3661 gaacataaat cattagattt tgtatgggta tggttaactt aatgtttatt atgataatta
     3721 agaatcacac ttacaataac atcaattaat aaatggttaa tcatttttga tatatcgtaa
     3781 gaataatgta gtttttaaca ccatccctgg tatctcaact atctctataa aacagcatga
     3841 cgctgtcgtc ctctggctct atcccagatg ccgtaaaaac gccctgcatt gctggcggta
     3901 taccagacag tatgacgaat attgcgatgc ccaagataat cctagataag tcgcgtatct
     3961 attcccatat tcgccaaagc aaaaccacac gaatagcgta acatgtgcgg atgaatctcc
     4021 agtgacaacc cggcattacc accagaagtc gagataatat ggtaaaactg ttgccgagaa
     4081 agcggattcc ccttacgtga taaaaatacc cactcgctct cagcatgcgg ataagaagta
     4141 cggatactca accagttttt taaagcctga acttctttgt tcaatagcgg gtgcgttgtt
     4201 gaaaagcctt tttttaatcg atggatatat atacactttg ctttaagatc aatatccgaa
     4261 atcctcaatc gacaaatttc actcgcccgg aaaccatgaa taaaacaaag caaaatcaga
     4321 caataattac gtgttgcatg aggcccggta tttgctgctt taaggagtga ctcgatttca
     4381 ctatgggtaa ggaagttcct ttttttgtta tcagccttat tcctcatcgt tttcccttat
     4441 aattacagac gcgcactagc tgtgctgggt tagcaataat ccaacatttt tatcctgatt
     4501 atgtttataa aagcgaacgt ttgcttaata acttacgtaa gtggaccagt tcttctgagc
     4561 gaacttaaat ggagtagcga tagttactta taataacttt gccatacaaa cgccatgctc
     4621 ttgcatgcta tgtaccttta tatatttatc aatctgatac gaatctaata ataaccctca
     4681 tcattaatgc aactaatctt atctatatat catgtgatat gttctgtaac aagtaaaatc
     4741 aacataaaaa attcagcaaa atttaatatt tgttaaacta gccgtctcaa atatgacatt
     4801 attagttaac tgcatttgcc acaaaatggc tatttcatgg aacgcagtaa tttcttagtg
     4861 gctattatca tgctaattct tagatcccta gcgattattc ctgcctggtt cgtcagctta
     4921 ggccatgaga ttgttacatt cctttttatc ctggagatat gggggaacgc aggcgacgct
     4981 ttttcatcta catccgttgg taaatatagc tttggtcact acgacttaag ccttcctcaa
     5041 tcacagctat tcgccgggcg atacggcaaa caaactaagt gaaacatcat cctgaaaaca
     5101 ccaacatcaa caagcctctc cagatcgaca tcaggagtga ccagttacaa gccacaaacc
     5161 aagaaacata tttcatgatg agaattatgc tcagcctgat ggcgagtaaa cccttatttt
     5221 caccactctc actgagttag cttgcaaagc tttctttgtt tagattctaa ataaattatt
     5281 tataaaacgt atagttaaaa gaaaatcttt agtttttgaa gaccaccgca agtgttcgtc
     5341 tggcttcaca tggcatcttc ttcttagaaa aagatcgaca tattttgtga cgcgaattgc
     5401 aaatctggtt ttgttgtatg gattgcgtga tttttgatct ggtataacag gtataaagat
     5461 gcaccacgat agtcaatgag actgggcatc tcgtaatcta tggcaaacat cacttcagtt
     5521 ctttcacatc gggtgatgaa aatgcacttc agtctgaaag gaatatgaaa atgagatcaa
     5581 cagacactct attttatgac tctgggtaaa atgggttgag taagtgatat aacttacgaa
     5641 tattcaaatc aattaaaaat cagaagagat tttatactca ggtatttaat ctggatctct
     5701 gtttatttaa ataatgttaa aagagaattt tcacaggaga ccttatacaa aaaatataaa
     5761 atacagttac ccgttgccaa agacactata agcctggcaa aaaaatatta cacaacataa
     5821 atgctaattg tttatgcggg ctttgtattg ctttctgtat cctacaaatg agtgaaattt
     5881 atgaaaaagg ctaaaatact ttctggcgta ttattactgt gcttttcgtc cccattaatt
     5941 tctcaggctg cgacactgga cgtacgtggt ggatatcgta gtggaagcca cgcctatgag
     6001 actcgactca aagtcagtga gggatggcaa aatggatggt gggcaagcat ggaaagtaat
     6061 acctggaata ccattcatga taataaaaag gaaaatgccg cactcaatga tgttcaggtt
     6121 gaagttaatt acgcaattaa acttgatgat caatggacgg tgcgcccggg aatgttaacg
     6181 cattttagca gcaacggcac acgctacgga ccctacgtaa aactgtcctg ggacgcgaca
     6241 aaagatctta attttggcat tcgctatcgt tacgactgga aagcttaccg acaacaagac
     6301 ttatccggtg atatgtctcg tgataacgtt catcgttggg atggatatgt cacttaccat
     6361 attaatagtg atttcacctt cgcatggcaa acgacgctat acagcaaaca gaatgattat
     6421 cgctatgcaa accacaagaa atgggcgacg gaaaatgcct ttgttctaca ataccatatg
     6481 acgcccgata ttacgccata catagaatat gactaccttg accgccaggg tgtttacaac
     6541 ggcagagata atttatcgga aaacagttat cgcattggtg tgtcatttaa actgtagtag
     6601 acaggagaca gtcacaatga ataaaactat aatggcgctt gctatcatga tggcttcatt
     6661 tgccgcaaac gcgtctgtat taccggaaac tcctgtgcca tttaaaagtg gtaccggagc
     6721 aattgataac gacactgtct acattggttt aggtagcgca ggtacagcat ggtacaagct
     6781 agatacacag gccaaagata aaaaatggac tgcgttagct gcattccctg gtggaccgag
     6841 agagcaagca acctctgcat ttattgatgg caatctgtat gtgtttggcg gcattggcaa
     6901 aaacagcgag ggattgactc aggtatttaa tgacgtacac aaatacaacc ccaaaaccaa
     6961 tagctgggtt aaattgatgt cgcacgcgcc gatgggcatg gcgggtcatg tgacttttgt
     7021 acacaacggc aaggcttatg ttactggcgg tgttaaccag aatatcttca atggctattt
     7081 tgaagatctc aacgaggctg gaaaagattc aaccgctata gataaaatca acgcccacta
     7141 ttttgacaaa aaagcagaag attatttctt caataagttt ctgttgtctt ttgatccctc
     7201 aacacagcaa tggagttacg ctggcgaatc gccctggtac ggaacggctg gtgcggcggt
     7261 tgtgaataaa ggtgataaaa cctggcttat taatggcgaa gccaaaccag gattgcgaac
     7321 ggatgccgta tttgaacttg atttcaccgg taataattta aaatggaata agcttgatcc
     7381 cgtctcatca ccagatggcg tcgctggcgg ttttgcgggg ataagcaatg attctcttat
     7441 atttgctgga ggggccggat tcaaaggttc acgagaaaat taccagaacg gtaagaacta
     7501 tgcgcatgaa ggcctgaaaa aatcatatag cactgatatt catctttggc ataacgggaa
     7561 atgggataaa tcgggtgaat tatcgcaagg tcgggcctac ggagtatcat tgccctggaa
     7621 taatagttta ttaattattg gcggtgaaac tgcaggcggc aaagcggtga cggattcagt
     7681 tttaatttct gtgaaggata ataaagtcac agtacaaaac taacgcttca gggccccggt
     7741 aaaggggccc tgctatcaat ttgtcatttg tattacgcga aaattatatg aacgcaataa
     7801 tatcacccga ttattactat gttcttaccg ttgctggtca gtctaatgcc atggcgtatg
     7861 gcgaaggact gccattaccg gacagggaag atgcgcctca ttccagaatt aaacaattag
     7921 cgagatttgc gcatacgcat cccggaggcc cgccatgtca ctttaacgac attattccac
     7981 tgactcactg cccacacgat gttcaggata tgcagggtta tcaccatcct ctggcaacga
     8041 atcatcaaac acagtacggc accgttggcc aggcactgca tattgcacgg aaattactgc
     8101 ccttcattcc tgataatgca gggattctta tcgttccgtg ttgccgtggc ggatcggctt
     8161 ttaccgcggg cagcgaaggg acatattcag aacggcacgg agccagccat gatgcttgtc
     8221 gttggggaac ggatactccg ctgtaccagg atttagtcag cagaacgcga gccgcactgg
     8281 caaaaaatcc gcagaacaaa ttcctcggcg tatgctggat gcaaggcgaa tttgacttaa
     8341 tgaccagtga ctacg
//
LOCUS       DAJTUP010000147         8331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTUP010000147 DAJTUP010000000
VERSION     DAJTUP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.149"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..158
                     /locus_tag="OO468_003628"
     CDS             <1..158
                     /locus_tag="OO468_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260599.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO468_003629"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO468_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR6260600.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO468_003630"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO468_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR6260601.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1245
                     /locus_tag="OO468_003631"
     CDS             874..1245
                     /locus_tag="OO468_003631"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR6260602.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1266..6127
                     /gene="traI"
                     /locus_tag="OO468_003632"
                     /pseudo
     CDS             1266..6127
                     /gene="traI"
                     /locus_tag="OO468_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6147..6893
                     /gene="traX"
                     /locus_tag="OO468_003633"
     CDS             6147..6893
                     /gene="traX"
                     /locus_tag="OO468_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR6260603.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6948..7508
                     /locus_tag="OO468_003634"
     CDS             6948..7508
                     /locus_tag="OO468_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR6260604.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7639..7851
                     /locus_tag="OO468_003635"
     CDS             7639..7851
                     /locus_tag="OO468_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR6260605.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8095..>8331
                     /locus_tag="OO468_003636"
     CDS             8095..>8331
                     /locus_tag="OO468_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR6260606.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1912 a         2086 c         2671 g         1662 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag
     1261 aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc
     1321 aggaaccaca gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat
     1381 ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta
     1441 atcagattgc ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg
     1501 gctatgaaac tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg
     1561 aggccttttc cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga
     1621 aatcccggga tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag
     1681 tcagaatggc cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc
     1741 gtgacgcagc ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg
     1801 acgggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag
     1861 tgcagtttat gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg
     1921 gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc
     1981 ccctcgatcg tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag
     2041 tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa
     2101 gtgtcccccg gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt
     2161 cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac
     2221 tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct
     2281 cgcagatgaa cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc
     2341 agttgctgga aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa
     2401 aactctccct gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg
     2461 tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg
     2521 atgccggggt gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca
     2581 gtgaaccgga ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga
     2641 agagagcgtg gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg
     2701 cagtgagctg aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc
     2761 accggtctgg ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt
     2821 gatggagcag tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac
     2881 ggcgcagagt cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat
     2941 ttcttccctg gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg
     3001 tgagcgtctg agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct
     3061 gcaggtggca tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc
     3121 ggccaccctg cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt
     3181 ggaaacgccc gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat
     3241 ggcaatggac aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta
     3301 ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt
     3361 ggtttctgag cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct
     3421 gcagaaagcc gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct
     3481 ggaaagtaaa aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc
     3541 tgcagaagga accggttttg ctgacctggg aggggaaatc aatgcgcaga taaaacgggg
     3601 tgatttactg tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc
     3661 gtcgtatgag gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac
     3721 gccgctgatg gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc
     3781 cgccacccgt atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg
     3841 tgtgggtaag accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag
     3901 tgaacgtccc cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag
     3961 cgccggcgtg gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg
     4021 cagcggagaa actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt
     4081 gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt
     4141 ggccagcggt gacacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca
     4201 gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga
     4261 actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct
     4321 tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc
     4381 cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat
     4441 gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga
     4501 ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga
     4561 ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg
     4621 gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg
     4681 tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca
     4741 ccggattgcc ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa
     4801 cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga
     4861 caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg
     4921 ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc
     4981 tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga
     5041 cctggcctat gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc
     5101 gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc
     5161 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     5221 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     5281 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     5341 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     5401 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     5461 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     5521 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     5581 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     5641 ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa
     5701 tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     5761 gggagccgga aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     5821 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     5881 caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga
     5941 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga
     6001 accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg
     6061 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     6121 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     6181 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     6241 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     6301 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     6361 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     6421 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt
     6481 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     6541 cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt
     6601 ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc
     6661 gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg
     6721 cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga
     6781 ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt
     6841 tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt
     6901 ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga
     6961 agcgaccggt actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga
     7021 aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg
     7081 agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
     7141 ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc
     7201 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
     7261 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
     7321 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
     7381 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc
     7441 gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
     7501 agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat
     7561 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     7621 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     7681 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     7741 gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt
     7801 gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     7861 cagccttcat tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa
     7921 tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa
     7981 gtcgattttt tatattttgt gattagtttc acaaaataag gtgttattca atgtgtgctg
     8041 cactattcag gggggctgaa atacaggtgg catttatctg atatggagaa taacatgagg
     8101 aaatatattc cactggcatt atttatcttt tcatggccgg tattgtctgc agatattcat
     8161 ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg
     8221 gtgcggatca ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg
     8281 tcaacagaca tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac a
//
LOCUS       DAJTUP010000148         8300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTUP010000148 DAJTUP010000000
VERSION     DAJTUP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.150"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            164..475
                     /locus_tag="OO468_003637"
     CDS             164..475
                     /locus_tag="OO468_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6260607.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            570..1103
                     /gene="ymdB"
                     /locus_tag="OO468_003638"
     CDS             570..1103
                     /gene="ymdB"
                     /locus_tag="OO468_003638"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR6260608.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1045..2526
                     /gene="clsC"
                     /locus_tag="OO468_003639"
     CDS             1045..2526
                     /gene="clsC"
                     /locus_tag="OO468_003639"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR6260609.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2534..3691)
                     /gene="mdoC"
                     /locus_tag="OO468_003640"
     CDS             complement(2534..3691)
                     /gene="mdoC"
                     /locus_tag="OO468_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR6260610.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4085..5620
                     /gene="mdoG"
                     /locus_tag="OO468_003641"
     CDS             4085..5620
                     /gene="mdoG"
                     /locus_tag="OO468_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR6260611.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5613..8156
                     /gene="mdoH"
                     /locus_tag="OO468_003642"
     CDS             5613..8156
                     /gene="mdoH"
                     /locus_tag="OO468_003642"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR6260612.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2094 a         1972 c         2167 g         2067 t
ORIGIN      
        1 tcatttatca caacaatgga cgccctcttc agaaaagtct taatttgttg aaatatcgag
       61 cataagatga atctggagag aatggtctgc tgcgaatcag ccaacctgaa agtatggata
      121 acacaacctt caaggatgac taatcattga ggaaatagaa taaatgttca gacctttttt
      181 agactctctt atgctcggca gtttgttttt tccttttatt gccattgcag gaagcaccgc
      241 gcaagggggc gtgatccatt tttatggcca aattgtggaa ccggcatgtg acgtcagcac
      301 ccagtcatca cccgtagaaa tgaactgccc acagaatggt tctattccgg gcaaaaccta
      361 ctccagcaaa gcgttaatga gcggcaatgt caaaaacgcg caaatagcct cagtaaaagt
      421 gcagtatctt gataaacaga aaaagctggc tgtaatgaac atcgaatata actaagtttc
      481 tgaacaactc acgttgctga gcagaaaaat gcgatttaac caaaaagcct gctgtacact
      541 taagaaacaa gaaggtgtaa ggaggcgtta tgaaaacgcg tattcatgtt gtgcagggtg
      601 atattaccaa actggccgtt gatgtgattg tgaatgcggc taatccgtca ttaatgggag
      661 gcggcggcgt cgatggggcc attcatcgcg cagcgggtcc ggccctgctg gatgcctgtt
      721 taaaagtcag gcaacagcag ggcgattgcc ctacggggca tgccgttatt acgcttgcag
      781 gcgatcttcc cgctaaagcc gtagtacaca ccgtcgggcc agtctggcgt ggtggggaac
      841 aaaacgaaga ccagcttttg caggatgcct atctgaatag cctacgactg gtggcggcaa
      901 acagctatac gtcagtggct tttcctgcaa tcagtactgg ggtttatagt taccctcgtg
      961 cggcagcggc tgaaatcgca gttaaaaccg tttcagaatt tattacccgt cacgctttac
     1021 ccgaacaggt atactttgtc tgttatgatg aagaaaacgc ccacctctac gaaagactcc
     1081 ttacccaaca aggagatgaa tgatttgccc cggctggcga gcgcggtgct gccactgtgt
     1141 tcgcaacatc ccggtcagtg tggtcttttc cctctggaga aaagtctgga tgcgtttgcc
     1201 gcccggtatc gtctggccga aatggcagag catacgctgg atgttcagta ttacatctgg
     1261 caggacgata tgtcggggcg gttactgttt tccgccctgt tagccgcagc aaagcgtggc
     1321 gttcgcgtcc gtttgttgct ggacgacaac aatacgcccg gacttgacga cattttgcgc
     1381 ttgcttgaca gtcatccacg cattgaagtc cggcttttta atcctttctc gtttcgcttg
     1441 ctacgtccgc ttggatatat caccgacttt tcccgtctca atcgccggat gcacaataaa
     1501 agtttcactg tcgatggcgt ggttacccta gtgggaggac gaaatattgg tgatgcctat
     1561 tttggtgcag gggaagagcc acttttttcg gatttagatg tcatggcaat aggccccgtg
     1621 gtagaggatg ttgccgatga tttcgcccgc tactggtatt gcaaatcggt ttcaccctta
     1681 cagcaggtgc tggatgtccc ggaaggtgaa atggcggatc gcatcgagtt acccgcctcc
     1741 tggcataacg atgccatgac gcatcgttat ttacgcaaaa tggaatccag tccatttata
     1801 aatcatctgg ttgatggaac gttgccgctt atctgggcga agacacgttt attaagtgat
     1861 gatccggcga aaggggaggg caaggcaaaa cggcattcac tgttaccgca gcgcctgttc
     1921 gatatcatgg gctcacccag tgaacgcatc gatattatct cttcctattt tgtaccgaca
     1981 cgcgcaggtg tggcgcaact cttacggatg gtgagaaaag gggtaaagat tgcgatccta
     2041 accaattctc ttgccgctaa cgatgttgct gtcgtccatg ccggatacgc gcgctggcgc
     2101 aaaaaattgc tccgctatgg cgtggaatta tatgaactca agccgacgcg tgaacaaagc
     2161 agtacgttac acgatcgcgg cataaccggt aattccggtg ccagcctgca tgctaaaact
     2221 tttagcatcg atggtaaaac ggtgtttatc ggctctttca atttcgatcc gcgttcaaca
     2281 ttgctcaata ctgaaatggg cttcgtgata gagagcgaaa cgctggcaca gttaattgat
     2341 aaacgcttta ttcagagcca gtacgatgcg gcctggcagc tccgtctgga caggtgggga
     2401 cggatcaact gggttgatcg tcatgcaaag aaagaggtta ttctcaaaaa agaacccgcc
     2461 accagtttct ggaagcgggt tatggtcaga ctggcgtcga tattgcccgt ggaatggtta
     2521 ttgtaaatgt ggcttaacgg gctggtgctt tatcgttctc acgcttaaca accggtttac
     2581 cagagaacaa aaacttcagt aacgggatgc gtaaatgaat ttcatacaga attatcgcaa
     2641 tccctactac gaatatcagg ccacagagaa aaccaagcca gttggaggtg atgtgcggtg
     2701 taatgtatgc gccgaaaaac agcgttaacg ggtggtgaac cagatagata aacagcgatg
     2761 cgttaacaaa ataagtcacc cgcgctgact ggaagttaag caaacggtgg ccaaaggaga
     2821 agaccacatt caccatccac aggccgagga ccatggtgat caccgactcg gtttcgtaca
     2881 tccaggcatc gccactgcca tagcgctggt tgagtaaata agcgacaaac gccaatgctg
     2941 cagcgagggt acagccacga gacggcgtgg taaacaaggc tttaagatga gggaaaatga
     3001 aagccagtgc gccgaggata aagaacggta aataaaatag cgtttgcatg acaataaaat
     3061 tgaacatgcc attactcaga atgggtggat acacaataaa aatcgttctt cttattaccg
     3121 catagccgat gccgaggcat aaaaaaatca ccgatagttt taccatcgag aattttttat
     3181 tcgttttatc agaattttct aaattatttc tgatgcgctt aaatatccat acacacagcg
     3241 tcgtcatgac tactaacacc agtaaaaacc acaggtgtga tattaattcc caggccaacg
     3301 tattatattt gtcatacaat gatagcccag gccaactttc cgctttccct ttgacatatt
     3361 gcagcataat aaattgcggt aatgtcagta gggggatggc tgttaacatc gggataccta
     3421 cacgttcgac acgtactttc caccattttt tcaagggata gcgtaaaaaa agcatgtagg
     3481 aaaagtatcc ggatataacg aaaaatacct gcatgcggaa cgagtggatg aagtcattaa
     3541 aaagggttag ccacaatgac ggttcggcgc tattcacatg ccatgtatgg ctcgaataga
     3601 ttaaagaaat atgaaaaggg atccctaaca acatcagcca ggcgcggatg gagtcgagga
     3661 aatattcacg ttgcgcgggt actggattca tatatggtta actaatctcg gatttttcgt
     3721 cttatccctg tcgggttatg cctttaggct tgttgccata gcgacaccga cctgaccgcg
     3781 ccaggcgcag gcttcaaggt ttttatgcat agcatcatcg ctaccactaa ccagaatgga
     3841 agcgtctgta agacggttga taaataaatt tgctggcaaa ccctacacga agtcgatgct
     3901 tctgtcttta ggagaagcac ggaaagtgaa aacggttgca atcaggtgct taatccatga
     3961 gccagcgtgc tgaacgatac cgggattcag ttgtcggaat ggctggttat ccattaaaat
     4021 agatcggatc gatataagca cacaaagggg gaagtgctta ctaattatga aacataaact
     4081 acaaatgatg aaaatgcgtt ggttgagtgc tgcagtaatg ttaaccctgt atacatcttc
     4141 aagctgggct ttcagtattg atgatgtcgc aaagcaagct caatccttag ccgggaaagg
     4201 ctatgaggcg cctaaaagca acttgccctc cgttttccgc gacatgaaat acgcggacta
     4261 tcagcagatc cagtttaatc atgacaaagc gtactggaac aatctgaaga ccccattcaa
     4321 actcgagttc taccatcagg gtatgtactt cgataccccg gtcaaaataa atgaagtgac
     4381 tgccaccgca gtcaaacgaa tcaaatacag cccggattat ttcactttcg gcgatgttca
     4441 gcatgacaaa gacacggtaa aagaccttgg ttttgccggt tttaaagtgc tttacccgat
     4501 caacagcaaa gataaaaacg atgaaatcgt cagcatgctc ggggccagct atttccgcgt
     4561 gattggtgca ggtcaggttt atggcctttc tgcccgcggc ctagcaattg ataccgcttt
     4621 gccatcgggt gaagaatttc cacgcttcaa agagttctgg atcgagcgtc caaaaccgac
     4681 tgataaacgt ttaaccattt atgcattgct tgactcgccg cgtgcgacag gtgcttacaa
     4741 attcgtggtt atgccagggc gtgacacggt tgtggatgtg cagtcgaaaa tctatctgcg
     4801 cgataaagtc ggcaaactgg gggttgcacc gttaaccagt atgttcctgt ttgggccgaa
     4861 ccaaccgtcg cctgcaaata actatcgtcc ggagttgcac gactctaacg gtctgtctat
     4921 ccatgctggt aatggcgaat ggatctggcg tccgttgaat aacccgaaac atttagcggt
     4981 cagcagcttc tccatggaaa acccgcgagg ctttggtctg ttgcaacgag gtcgtgattt
     5041 ctcccgcttt gaagatctcg atgatcgtta cgatctccgt ccaagcgcat gggtgactcc
     5101 gaaaggggag tggggcaaag gcagcgttga gctggtggaa attccaacca acgatgaaac
     5161 caacgataac atcgtcgctt actggacgcc ggatcagctg ccggagccgg gtaaagagat
     5221 gaactttaaa tacaccatca ccttcagccg tgatgaagac aaactgcatg cgccagataa
     5281 cgcatgggtg caacaaacgc gtcgttcaac gggggatgtg aagcagtcga acctgattcg
     5341 ccagcctgac ggtactatcg cctttgtggt cgattttacc ggcgcagaga tgaaaaaact
     5401 gccagaggat accccggtca cagcgcaaac cagcattggt gataatggtg agatagttga
     5461 aagcacggtg cgctataacc cggttaccaa aggctggcgt ctggtgatgc gtgtgaaagt
     5521 gaaagatgcc aagaaaatca ctgaaatgcg tgctgcgctg gtgaatgccg atcagacgtt
     5581 gagtgaaacc tggagctacc agttacctgc caatgaataa gacaactgag tacattgacg
     5641 caatgcccat cgccgcaagc gagaaagcag cattgccgaa gactgatatc cgcgccgttc
     5701 atcaggcgct ggatgccgaa caccgcacct gggcgcggga ggatgattcc ccgcaaggct
     5761 cggtaaaggc gcgtctggaa caagcctggc cagattcact tgctgatgga cagttaatta
     5821 aagacgacga agggcgcgat cagcttaagg cgatgccaga agcaaaacgc tcctcgatgt
     5881 ttcccgaccc gtggcgtacc aacccggtag gccgtttctg ggatcgcctg cgtggacgcg
     5941 atgtcacgcc gcgctatctg gctcgtttga ccaaagaaga gcaggagagc gagcaaaagt
     6001 ggcgtaccgt tggtaccatc cgccgttaca ttctgttgat cctgacgctc gcgcaaactg
     6061 ttgtcgcgac ctggtatatg aagaccattc ttccttatca gggttgggcg ctgattaatc
     6121 ctatggatat ggttggtcag gatttgtggg tttcctttat gcagcttctg ccttatatgc
     6181 tgcaaaccgg tatcctgatc ctttttgcgg tactgttctg ttgggtgtcc gccggattct
     6241 ggacggcgtt gatgggcttc ctgcaactgc ttattggtcg cgataaatac agtatatctg
     6301 cgtcaacagt tggcgatgaa ccattaaacc cggagcatcg cacggcgttg atcatgccta
     6361 tctgtaacga agacgtgaac cgtgtttttg ctggcttgcg tgcaacgtgg gaatcagtaa
     6421 aagccaccgg gaatgccaaa cactttgatg tctacattct gagtgacagt tataacccgg
     6481 atatctgcgt cgcagagcaa aaagcctgga tggagcttat cgctgaagtc ggtggcgaag
     6541 gtcagatttt ctatcgccgc cgccgtcgcc gcgtgaagcg taaaagcggt aatatcgatg
     6601 acttctgccg tcgctggggc agccagtaca gctacatggt ggtgctggat gctgactcgg
     6661 taatgaccgg tgattgttta tgcggcctgg tgcgcttgat ggaagccaac ccgaacgccg
     6721 ggatcattca gtcgtcgccg aaagcgtccg gtatggatac gctgtatgcg cgctgtcagc
     6781 agttcgcgac ccgcgtgtat gggccactgt ttacagccgg tttgcacttc tggcaacttg
     6841 gcgagtcgca ctactgggga cataacgcga ttatccgcgt gaaaccattt atcgagcact
     6901 gcgcactggc tccgctgccg ggcgaaggtt cctttgccgg ttcaatcctg tcacatgact
     6961 tcgtagaagc ggcgttgatg cgccgtgcag gttggggggt ctggattgct tacgatctcc
     7021 cgggttctta tgaagaattg ccgcctaact tgcttgatga gctaaaacgt gaccgccgct
     7081 ggtgccacgg taacctgatg aacttccgtc tgttcctggt taagggtatg cacccggttc
     7141 accgtgcggt gttcctgacg ggcgtgatgt cttatctctc cgctccgctg tggtttatgt
     7201 tcctcgcgct ctctactgca ttgcaggtag tgcatgcgtt gaccgaaccg caatacttcc
     7261 tgcaaccacg gcagttgttc ccggtgtggc cgcagtggcg tcctgagctg gcgattgcac
     7321 tttttgcttc gaccatggtg ctgttgttcc tgccgaagtt attgagcatt ttgcttatct
     7381 ggtgcaaagg aacgaaagaa tacggcggct tctggcgcgt tacattatcg ttgctgctgg
     7441 aagtgctgtt ttccgtgctg ctggctccgg tacgcatgct gttccatacg gtcttcgttg
     7501 tcagcgcgtt ccttggctgg gaagtggtgt ggaattcacc gcagcgtgat gatgactcca
     7561 cttcatgggg cgaagcgttc aaacgccacg gctcacagct gctgttgggg ttagtgtggg
     7621 ctgttgggat ggcgtggctg gatctgcgtt tcctgttctg gctggcaccg attgtcttct
     7681 cgttgatcct gtcaccgttt gtttcggtga tttccagccg tgccaccgtt ggtctgcgca
     7741 ccaaacgctg gaaactgttc ctgatcccgg aagagtattc accgccgcag gtgctggttg
     7801 ataccgatcg gttccttgag atgaatcgcc aacgctccct tgacgatggc tttatgcacg
     7861 cagtgtttaa cccgtcattt aacgctctgg caaccgcaat ggcgacagcg cgtcaccgcg
     7921 ccagtaaggt gctggaaatc gcccgtgacc gccacgttga acaggcgctt aacgagacgc
     7981 cagagaagct gaatcgcgat cgtcgcctgg tgctgctaag cgatccggtg acgatggccc
     8041 gtctgcattt ccgcgtctgg aattcccctg aaagatattc ttcatgggtg agttattacg
     8101 aagggataaa gctcaatcca ctggcattgc gtaaaccgga tgcggcttcg caataaaaac
     8161 gtagttgcct gatgcgctac gcttatcagg cctacatcgt tcctgcaatt tattgatttt
     8221 gcaagatttt gtaggtcgga taaggcgttc acgccacatc cggcaaaaac aacgagccaa
     8281 taaaaatacc ggcgttatgc
//
LOCUS       DAJTUP010000149         8250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTUP010000149 DAJTUP010000000
VERSION     DAJTUP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.151"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(71..2233)
                     /gene="uvrD"
                     /locus_tag="OO468_003643"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(71..2233)
                     /gene="uvrD"
                     /locus_tag="OO468_003643"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR6260613.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2317..3033)
                     /gene="yigB"
                     /locus_tag="OO468_003644"
     CDS             complement(2317..3033)
                     /gene="yigB"
                     /locus_tag="OO468_003644"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR6260614.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3033..3929)
                     /gene="xerC"
                     /locus_tag="OO468_003645"
     CDS             complement(3033..3929)
                     /gene="xerC"
                     /locus_tag="OO468_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR6260615.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(3926..4633)
                     /locus_tag="OO468_003646"
     CDS             complement(3926..4633)
                     /locus_tag="OO468_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR6260616.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4630..5454)
                     /gene="dapF"
                     /locus_tag="OO468_003647"
     CDS             complement(4630..5454)
                     /gene="dapF"
                     /locus_tag="OO468_003647"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR6260617.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5491..5694)
                     /locus_tag="OO468_003648"
     CDS             complement(5491..5694)
                     /locus_tag="OO468_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR6260618.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            5883..7361
                     /locus_tag="OO468_003649"
                     /pseudo
     CDS             5883..7361
                     /locus_tag="OO468_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7358..>8250
                     /locus_tag="OO468_003650"
     CDS             7358..>8250
                     /locus_tag="OO468_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR6260619.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         1908 a         2287 c         2116 g         1939 t
ORIGIN      
        1 acgttgcaat ttattgaatc tgaatgattt tttaggccaa ataaggtgcg cagcaccgca
       61 tccggcaacg ttacaccgac tccagccggg cgtaagctgc caccagccat ttgattccct
      121 ggccctggaa tgccacctgc aaacggctat gttcaccgct gccttccata ttgacgatgg
      181 tgccttcacc aaacttagcg tggcgtacac gctggccgag cttgtagccg ctgtcgttct
      241 cgaccatcgg cgtacccatc cgctgatggc tgaccgggcg gcttaccgtg gcgcgcaggc
      301 gcacctcttc cacacactct tccggcaact cgccaataaa gcgcgacggg cgatggtaaa
      361 cctctttgcc atacagacgg cgagtttccg cgtaggtcag cgtcagtttc tgcatcgcgc
      421 gggttacgcc aacgtaggcc agacggcgtt cttcttccag acgcccgcct tcatccagcg
      481 acatctggct tgggaacatg ccctcttcca taccgacgat aaacacctgc gggaactcca
      541 gacctttcgc cgagtgcatg gtcatcagtt gtaccgcatc ctgccaggta tccgcctgcc
      601 cttcgcctgc ttccagcgcc gcatgggata ggaacgcctg cagcggcatt aaatcttcgt
      661 cttcttcgtt gtagctgaac tggcgcgttg ccgtcaccag ttcctctaag ttttcgatac
      721 gcgtctgacc tttttcgccc ttctcctgct cgtacatggt acgcaggccg gagtctttaa
      781 ttacccggtc agtctgtaca tgcagcggca tatcggcagt ttcctgcgct aaggcgtcga
      841 tcagttccat aaaccgctgt aaggcgctgg cagcacgtcc ggcgagggct ttttcctgca
      901 acagttcacg acatgcctgc cagagtgtta actggcgatc gcgcgatgtc tgacgtacca
      961 cgtccagcgt ccggtcacca ataccccgcg ttggcgtatt caccacacgc tcaaaggccg
     1021 cgtcgtcgtt acggttggca atcaggcgca gatacgagag cgcatctttg atttcctggc
     1081 gttcgaagaa gcgcatcccg ccgtaaatac ggtacggcat actcgcctgc aacagtgctt
     1141 cttccagcac acgcgactgg gcgttactgc ggtagagaat ggcgcactcg gcaagcgccc
     1201 cgccgttgtc ctgccaggtt ttgatgcggt taaccacaaa acgcgcttca tcgagttcgt
     1261 taaaagcgca atagagggaa ataggctcac cgtccgcgcc atcggtccac agttttttac
     1321 ccagacgccc gttattgttt tcaatcaggg cgttagcggc gctcagaata ttgctggtag
     1381 agcggtagtt ttgctccaga cgaataattt cggcaccggg gaaatcatta aggaaacgct
     1441 gaatattctc cacctgcgcc ccgcgccagc cgtagattga ctggtcgtca tcaccgacga
     1501 tcatcacttt gccggtgtcg cccgccagca ggcggatcca cgcgtactga atgttgttgg
     1561 tatcctggaa ttcgtccacc aggatattgg taaaacgttc gcggtagtgt tgtaggatat
     1621 gcggcttgtt aagccacagt tcgtgagcgc gcagcagcag ctcggcgaag tccaccaggc
     1681 ccgcgcgatc gcacgcttcc tgatacgcct gatacacctt ctgccaggtc tgctccaccg
     1741 gattaccgta gctttgaata tgatgcggac gcaggccttc atctttctgg ctgttgatgt
     1801 accacattgc ctggcgcggc ggccactgct tctcgtcgag gttcatggct ttgatcagac
     1861 gcttaagcag gcgtagctgg tcttcgctat cgaggatctg gaaatcctgc ggcagattgg
     1921 cgtccatatg gtgcgcacgc agcaggcggt gcgccagccc gtggaaggtg cctacccaca
     1981 tgccgccctg gctggtgccc atcagttgtc cgatacgatg acgcatctcc gccgccgctt
     2041 tgttggtaaa cgtcacagcc ataatcgagt atggcgagca gttttccacg ctcatcagcc
     2101 aggcgatacg atgcaccagt acgcgcgtct taccactgcc cgcgcccgcc agcaccagaa
     2161 ggttgctgcg tggcgcggcc accgcttcgc gttgtttgtc attaaggctg tcgagcaggt
     2221 aagaaacgtc cattggcacc gccgcgtaaa aataggttgg gcggagaagc gcaacgccct
     2281 ccgccaaaaa gctgggtata tatacagatt tgctgattat atcagcgagg tcagagatgc
     2341 caaccgggaa atttccagat gtggcagtaa acggctgtcc caggtttgca tcagatcgcc
     2401 attttccggt ctgatccaac aagcctgcat tccgctgcga attgccccac ccacgtcagt
     2461 ggtgagatcg tccccaacat gtaagatctc gccgatcggc acgttgagtt tttccgcagc
     2521 caaaaagtac atatcgctga acggttttga gcgcccgtgc gggccagcgc gcagcacaaa
     2581 ctcaaaataa tcccccaaac caaacagttc cggctgggcg ttaccgttgg tgatcgccac
     2641 cagcggccat ttcttcgcca gctgttttaa ggtgtcgtga gtttgctgcg ggacgtcgat
     2701 tcggctgcgc catttggcaa agttaatcat tgctgcgtgt gcgcctgcac tggcttcttc
     2761 ggcactcagc ccggcgtcga gcatcgcttg ttcaatcgaa cgaaaacgcc aacgtgtcac
     2821 gtcgtgataa atctcgggtt ccgcttcccg taccgcctgg cgcaggcgtt gcagatcttc
     2881 attctggaag ctgcgcagcg ccggatgata attttgcaca aaggtaagcg cctctcgctc
     2941 ggtgcgcaaa atcaccggac ggttatcgta aagggtatca tccaggtcaa aggtgagcgc
     3001 cgagatgcgc cccaaaggcc ggtaaaaacg cattatttcc cccgtttggc gcgtggatgc
     3061 gccgcatcgt acaccgaggc aaggtgttga aaatcaagat gagtatagat ttgcgtggtg
     3121 gagaggttgg catgacccag tagctcctgc acaccacgaa gatcgccgct cgactccagc
     3181 atatgcgtag cgaacgagtg acgtaattta tgtgggtgaa cgtgattatt cagcccttgt
     3241 tttatgcccc attcggcaaa gcgtttctgc acattacgcg cggagatacg cttgcccagt
     3301 ttcgacagaa aaagcgcgtc gtcttcgcta ccaaacaggt cgcgcaaatc aagccagtgc
     3361 tcaatccacg ccacagcgtt gcgaccaatc ggcaggcggc gctctttgct gcctttcccc
     3421 atcacccaca cttcgccgga ctccaggtcg aggtgtttga tatccagccc caccagctcg
     3481 gaaagacgca gacccgcgcc gtacatcact tccagcattg cacggtcgcg tacagcgagg
     3541 ggatcgttga tatcaatatc cagcagccga ttcatatcgt cgacgtcgat atttttcggc
     3601 agatgacgcg gcgctttcgg tgccgaaact cctttcgccg ggttagcttt gagttcgttc
     3661 tggctgacca gccagtcaaa aaagctacgt agcgcagaaa gccgtaacgc cagacttgct
     3721 gctcccagcc ctttacggcg actgcgtaca gcaaaattgc gtaccatcgc tgcatcgcat
     3781 tgttgccagc tttgcaggcc gttttcgctg gcaaaattga tgatcgcctc aagctgacgc
     3841 tggtagttaa gtagggttat cgggctaagc tgacgctcca cgctcagata acgtaggtag
     3901 cgttctacat cggtgtgtaa atcggtcata cgcgttcaat ccaacgctcc agaagctccg
     3961 gcaacatcag cgcaatttca tgaagtaact gcgttccctg cccttgttga tagtgactgg
     4021 catcgcgact ggtaaacagc acaacaccca aatcagcatc gcttcccagc atcgacatcg
     4081 ccaccgatcc caccgctttc gcttccggta gcaccaccag cagctccggt ccgttaagcg
     4141 gcccaagata gtgctgttcc tgccccaaac gctgaatacg cagcggttcg aaagactgac
     4201 ggcttaatgc cagatgagtg tggttcgacg gcgcacctaa gcgccagcga tctggaaaca
     4261 ggcgcagact cgcaccagct aggccgagat cgcgcgccca gcggtgaaag cgcatcagca
     4321 tatcgtcgag actgctggcg gcggtgagac tgcgctgcag gtagagtagg cgataaaaca
     4381 gcccttcgtt ggcgatagcc tgctccatca acaacgccat gttctcttcc agaacatgaa
     4441 tgtgattacg tgcgcgggcc atgtgccact cgaccaacga aacggtgccg cgtaccggat
     4501 gcggcacacg tatcgcttct actgcgcgcg cattgcggat aaaaaactca ggatttttaa
     4561 tcagataatc gacaaccgcc cggtcatcaa gctccgtgag tgtttcctgc agttcttccc
     4621 ctggttgctt catagatgaa taaatccatc gtagacatgt accgccgggc cagtcatata
     4681 taacgggtga cccggacctt tccaggcgat atcaagacga ccgccgggga gttccacgcg
     4741 tacttcttcg tccagcaaac cttgctgaat ccctaccgcc accgccgcac acgcgccgct
     4801 gccgcaggcc tgggtttctc ctgccccacg ctcataaacg cgtaaacgaa tatgctcgcg
     4861 cttaaccact tgcataaaac cgatattggc gcgctccgga aaacgctcgt gactttccag
     4921 aacaggacca agcgtttcta ccgccgcggt atcgacatca tcgacctgaa tcacgcaatg
     4981 cggatttccc atcgacacca cgccgcataa gattgtctgc tcggcggcgc gcataatata
     5041 ggtcttttcc gctttgttag cgcgaaacgg cacggcggaa ggttcgaagt tgggttcgcc
     5101 catatttacg cggaccagat catcatcggt gacggtcaga accatccgcc cgttggcggt
     5161 gctgacgcgg atatcacgct tattggtcag tcctttcaga cgcacaaaac gggcaaagca
     5221 gcgcgcaccg ttaccgcact gcgccacttc actgccatca gcattgaaaa tgcgatagtg
     5281 aaaatccagt tcaggatcat acggcggctc aaccaccagc agctggtcaa accctacccc
     5341 caggtgccga tccgccaggc gacgaatcag ctccggtgaa aaaaagacat tctgcgttac
     5401 cgcgtcgacg accataaaat cgttgccaag gccatgcatt ttcgagaact gcatcattta
     5461 ctccaatcac gcgggtacag aaactgactt ttagtaattc acctgggatg gaccatcgcc
     5521 agtggcgcga tcgtttttat ccggcaccgt ggattgcgtt tgcgtctcta ccggtttggt
     5581 cggcggcggt gcgtttttat ctgcaggcgg gaaatagagc ggacctttca gaccgcagcc
     5641 cgtcaggctg aagagagtaa gtaatacagt gagtgcctta aacacgtttt tcattatgga
     5701 ttgcctgtaa agttcatcgc tttctgcttt ctatcatcgc aggagaaggc gtaaaagcaa
     5761 gcgtttagcg actctttatg aagtctgaaa agtgaggaaa tgctgcgagt ataacgctta
     5821 ctcgtgagga tttatgttct gctggcagtt tcgtgatcaa cacctcgcgc caggatggct
     5881 ttatgcacta tcgcaatgtg gtttgtttgt tgtcttgttc attgttttta tcgtcggcat
     5941 ggggatgtcg gctttccgca cctgaacata atatttacca gaaacaggga aaaggcgtgg
     6001 tgtacctgcg cccttataaa gaaacaaatc tttctctacc tgacgtgaat tacaagagtt
     6061 tgcgtcggtt acccaattta ttaatagacc cgacaacact agacgaatgg gataaagaac
     6121 cgcctctgac tgatttgaca accgactacc tctatgaggg cgcacaggcc tggtatcctc
     6181 attattcatg gcatagcgat ggtcgcaata tcctctacgc gggtgaagtt gtgcaaaacc
     6241 ctcccggcac acctcccgtc gatgtcgcct cgttcaaggc atggggtgat tttgccgcag
     6301 ataaacacag tctctatttt gaaggcaagc gcaccgatga caatggtggt ggaaatagct
     6361 tggatatcaa aacgctgcat caggtggaat tccggcctcc ctgggatcct gacttactgg
     6421 ggctgatatt acgtgacgct aatttcttgt atatcaatgg gcatcgcctc gctgaccccg
     6481 agagtttccg tgtgctggcg cagaagtcct gggatcagcg aggaaagttt tccaccacgt
     6541 ttaacccctg cattgccgta ccatttggcc cctgggatac cctggctcgt acacggacca
     6601 aaatcctgct caacggcgaa cagcttgatg ccgacccgga caccttttcc gtcgtgcgct
     6661 ggatgcccgg ctcactcttg acctggcgtg ataaaaacgg gctgcagcgt aaagtcctcg
     6721 acaaggaaaa tctggcgtgg gatgaagatt taacaaagca ctgtctggat ttttctctgc
     6781 tggaaaagaa agtgttctgg cgtaaaggac ctgcttgtaa acaggaagaa ttacccggac
     6841 tcgatccgga gcagtttcaa cccatcagtg atgctgtcgc ccagcatcag gactcgcttt
     6901 ataccatcat cgaaacagag tctggtaacc gtaagctgga gatcgtgaaa cttgatgatc
     6961 ccaatcttat tatcaacaaa cgtttcaacg ccgggaaacg ccacggctat ttacttacgc
     7021 gtgccgaagg gtagccatac cattccggtt tacacgtgtt tgaatctgac ggaccgctga
     7081 tcttactgga taaccgttct ccggatgaac gcgaagccca tcttaatgac catccctttt
     7141 tgcgcagatg gtatgcccgc gataaccgct acatttacag ctttgatggc gcgcagctct
     7201 ggcgataccg caccgctgat ccgaaacaag ttcgcttaat ctggaaggaa caacattcgg
     7261 gatatgtcta tggcgtaaat tacaaaacgg gatatctgga cggaaaaatt actgatgacg
     7321 gcgaatttat tcctgccccg cgcaatgagg cgacaaaatg aagggtcaaa taatcacagt
     7381 tgcactcatc ctgttgggac acacatttct gacgcctgct gtacaggcca tagggttcga
     7441 ctattacaat gatcacggtg ttatgtctta cggtaaaggt tattgggggg ataagaaaat
     7501 cattgcccag tttccggaga tgagcagggc cggtttgcgc cttgtgaaaa atatctctgg
     7561 gaaaacgggt tatatacctt ccgatgaaat aagcatagaa aatgaggaat accactgggt
     7621 gacagacggc cgcgttattc tctggcgtgg caaaatcgtt agcaatccac cgggaacact
     7681 cactgtcgat attgccagct ttcaggctat gggccgcttc gcggtcgata aatatagcct
     7741 ctattttgac ggacagcgca ccgaaagtaa tagcggcgcg tcacgtgttg agctggcgac
     7801 actaaaagct atcgaaggta actctaccac gctggtggat agtaaaaatc tctacttgtc
     7861 cggtcgacgt caggggagca gcagtgatgt tactgtatta gaaaaaagat ggtggggtat
     7921 taatccacgt cttatgagtg taaacagaaa ttcatattcc aatgatctgc ttatccgcag
     7981 tgggcagaat atttatttaa atggcgttca ccttacggcg aatgcagact catttgagat
     8041 aattcgctgg atacctcatt cactgctggt ttttcgcgac aataagggtc tgcatcgtta
     8101 tccctttggt caattatcag gcaaagcgat acccgtagat gatgacgtct cttttgaagt
     8161 aggggaaagt cgcgttcgct ggcgtaaaca gctcacgccc gaccgtcagt ggagcaagtg
     8221 gatagaccta ccaggtattg aacctgaaca
//
LOCUS       DAJTUP010000150         8181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTUP010000150 DAJTUP010000000
VERSION     DAJTUP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.152"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..536)
                     /gene="rspA"
                     /locus_tag="OO468_003651"
     CDS             complement(<1..536)
                     /gene="rspA"
                     /locus_tag="OO468_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6260620.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWP"
     gene            complement(742..1068)
                     /locus_tag="OO468_003652"
     CDS             complement(742..1068)
                     /locus_tag="OO468_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR6260621.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1203..1544
                     /locus_tag="OO468_003653"
     CDS             1203..1544
                     /locus_tag="OO468_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR6260622.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1610..2139
                     /gene="speG"
                     /locus_tag="OO468_003654"
                     /pseudo
     CDS             1610..2139
                     /gene="speG"
                     /locus_tag="OO468_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2142..2852)
                     /locus_tag="OO468_003655"
     CDS             complement(2142..2852)
                     /locus_tag="OO468_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR6260623.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2960..3265
                     /locus_tag="OO468_003656"
     CDS             2960..3265
                     /locus_tag="OO468_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR6260624.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3464..3784
                     /locus_tag="OO468_003657"
     CDS             3464..3784
                     /locus_tag="OO468_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR6260625.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            4344..4922
                     /locus_tag="OO468_003658"
                     /pseudo
     CDS             4344..4922
                     /locus_tag="OO468_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(5608..6597)
                     /locus_tag="OO468_003659"
     CDS             complement(5608..6597)
                     /locus_tag="OO468_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260626.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(6682..7173)
                     /locus_tag="OO468_003660"
     CDS             complement(6682..7173)
                     /locus_tag="OO468_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260627.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(7494..7994)
                     /locus_tag="OO468_003661"
     CDS             complement(7494..7994)
                     /locus_tag="OO468_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260628.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(7984..8166)
                     /locus_tag="OO468_003662"
     CDS             complement(7984..8166)
                     /locus_tag="OO468_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260629.1"
                     /translation="MMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQR
                     FKSFAEALAGGASYDF"
BASE COUNT         2106 a         2103 c         1990 g         1982 t
ORIGIN      
        1 tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt cgacatgccg
       61 taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa tcccagctcc
      121 tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt atggcaataa
      181 accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat gttggcagct
      241 ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat cgtaaccgga
      301 ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat acggtgcgca
      361 tcgcgaccaa taagccgcgg acaaagatga tcctgcaaat aagaggccac ggaaagctca
      421 cgtccattga gggtggcatc cccaaggccc gtaataccgt cctcagtggt gatttttaat
      481 gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat cttcatgttc
      541 gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcta ctgccataca
      601 agtatgaaga tcgaaaaaag ccggagtgat cacaaaaaaa ggcgtatatt tgcgctgtga
      661 atggttgaca aaagatgaaa tagaatacct tttgtcagct gacacttcct cgtatcttat
      721 tgataaaatg gatttatgtt cctacgtgcg cccccagccc gcaacaatga tcaacattcc
      781 gcaaagcgca atcaacgcac ccgtccagtc ataaagactt agtttcacgc catccacaac
      841 gcgcagccac atcaatgccg tgcagacata aacgccacca taagccgcgt aaacacgccc
      901 actcgccgca ggatgcaacg ttaacaacca gacaaacagc gccagtgaaa tccccgccgg
      961 aagcaacagc cagatactgg cgtttcgttt taaccacaac cagggcagaa agcatccaat
     1021 aatttcacac agcgcagtag caaaaaatag taacgttgtt ttaatcatct ttgtcactta
     1081 ttgacatcat gtatagttat agggcgacat aatatcatca atataaacat cctcctggta
     1141 cgttttgcgt tcgcagtgga tggtgtagaa tcacctttaa tcattcatac agggaatgaa
     1201 ttatgaaaat cactctcagc aaacgaatcg gcctgctcgc ttttctgctg ccttgcgcac
     1261 tggcattgag cacaactgtt catgccgaaa ctaacaaact ggtgattgag tctggcgaca
     1321 gtgcacaaag ccgccagcac gccgctatgg aaaaagagca atggaatgac acgcgcaatc
     1381 tgcgccagaa agtgaataaa cgcactgaaa aagagtggga taaagccgac gccgcttttg
     1441 ataaccgcga taaatgtgag caaagtgcca acatcaatgc ctactgggag cccaatactt
     1501 tgcgctgcct ggaccgtcga actggccgcg ttattacccc ctaacctgtt attgatttaa
     1561 ggaatgtaag gacacgttat gccaagcgcc cacagttaag ctacgcccgc tggagcgtga
     1621 agatttacgc tatgtacatc aactcgacaa taacgccagt gtgatgcgtt actggtttga
     1681 ggaaccctac gaagcctttg ttgaactctc tgatctgtat gataagcgca tattcacgat
     1741 cagagcgaac ggcgctttgt ggtggaatgt gacggcgaaa aagccggtct ggtggagctg
     1801 gtggaaatta accatgttca tcgccgggca gaatttcaga taattatctc cccggagtat
     1861 caggggaaag gtctggcaac ccgtgccgcc aaattagcaa tggactatgg cttcaccgtt
     1921 ctcaatctct ataagctgta tctgatcgtc gataaagaga atgaaaaagc gattcacatt
     1981 taccgcaagc ttggctttac ggttgaaggt gaattgatgc atgagttttt tattaatggt
     2041 caatatcgta atgccattcg catgtgtata ttccagcatc agtatctggc agagcacaaa
     2101 acaccgggtc agactctcct gaagccgacc gcacaatagc attaataata atcgatcgta
     2161 tttttgatgg tgtaaacccg ttcgacggcg ggttttactc cttcatcaac aatgatcagc
     2221 tgacagtcca ctggattggc gtgactgtca tattcacagc tctgttttac attaccaact
     2281 cgttgattat ttagtaaagt aaccgctgtg taatctaatt ttttgatcgg atccgttgat
     2341 ggcgtggcgc tgacagataa tgttttgtcg ttacttttcg tggttttacc cagcggatac
     2401 ccctgatcat catagcgata ttccatctgc atttgtttgc tgctggcttt aatcacgaag
     2461 ccattatcat cggtttccca actcaccccg gcagaaggta attctgctag ctggcatttt
     2521 ccctgtaaac gtactctctt ctccagcgtc tcggcatcac ggtaataatt ggcgtccagt
     2581 accagagcga ccacggtatt attttccaga tccagtaatt cgagtgaatc aaaacagcct
     2641 tcttccgaca aagtcccaga aacacgtttc gtcacttcac cttgctcatc cattaatgtc
     2701 tgagtgaaat cttttaccgg accacgcagc ggatcaaaat cgaattcatt agagaaactg
     2761 gccatctcag gggtaaatga aagtgttacc tctgtgcggt cacatcctgt gaggaatatc
     2821 gcgagtaagc atggtagtaa tttgtatttc acaacagtca ccagagagta gagatgattc
     2881 tcaatcatag tagcaaatac agtactttac acgttaaatg ctatgcttaa agaagttatc
     2941 ttcgcgtaag gagcttatga tgaaactctc aacctgctgt gccgcgcttc tgctcgccct
     3001 cgcctcaccc gcggtactcg ccgcgccggg atcttgtgag cgcatacaga gcgatatatc
     3061 acagcgcatt atcaataatg gcgtacccga aagcagcttc acgttaagta ttgtacccaa
     3121 tgaccaggtt gatcagcctg attcccaggt cgtcgggcat tgcgctaatg atacgcataa
     3181 aattctctac aaccgcacca ccagcggtaa cgtctctgct cccgcgcagt ctacccagga
     3241 tggcgcgcct gccgaaccgc agtaacacat tatcgactga acgccggata tgacacatcc
     3301 ggcgatttga acatacaaca taatctcacc ttattactca tacccttcta ttgatgtgga
     3361 ttaataattc ttaacccaaa atgggtagac tccctctatt gttagcgcgc taaatattca
     3421 atgtataaac atttatataa caataaagaa cagggagtga gttatgtcca aaaatgaccg
     3481 aatggtgggc atcagccgca gaacactcgt taaatcaacc gcgacaggtt ctctggcgct
     3541 ggctgcaggc ggtttttctt taccgttcac cctgcgcagt gcagcggcag cggtacaaca
     3601 ggccagcgaa aaagtgatct ggggttcctg ttccgtcaac tgtggtagcc gctgtgcact
     3661 tcgtctacat gttaaagata atgaagtgac ctgggtggaa actgacaata ccggcagcga
     3721 tgagtacggc aaccatcagg gtacgcgcct gtttgcgcgg tcgctccatc cgccggcgta
     3781 ttaatcatcc cgatcgcttg aatgacccaa tgaaacgcgt gggtaaacgc ggtgaaggca
     3841 aattcgaacg gattagctgg gatgaagccc tggatactat cgccagtagc ctgaaaaaaa
     3901 ccgtcgaaca atatggcaat gaagctgtat atattcagta ctcttcgggg attgttggcg
     3961 gcaatatgac ccgctcttcg ccatcagcct cggcggtcaa acgcctgatg aactgctacg
     4021 gcggttcact caatcagtat ggctcctaca gcaccgcgca aatttcctgt gccatgccct
     4081 acacctacgg cagtaatgat ggcaacagca ccacggatat tgaaaacagc aagctggtcg
     4141 tgatgtttgg taacaacccg gcagaaaccc gcatgagcgg tggtggcatc acttatcttc
     4201 ttgaaaaagc gcgcgagaaa tcgaacgcca aaatgattgt tatcgatccg cgatataccg
     4261 atacggctgc cggtcgtgaa gaggaatggc tccctattcg cccgggcacc gatgccgcgc
     4321 tggttgcggg tattgcctgg gtattgatta acgaaaatct cgttgatcaa ccttttctcg
     4381 ataaatactg cgtcggctat gacgaaaaaa ccttacccgc agatgcaccc aaaaatggtc
     4441 actataaagc ctatattctt ggtgaaggtg acgataaaac agcaaaaacg ccgcagtggg
     4501 cttcgcaaat taccggtatc ccggtagacc gtatcatcaa actggcgcgt gaaattggca
     4561 caacaaaacc cgcttatatc tgctagggct gggggccgca acgccaggca aacggcgagc
     4621 tgactgcacg cgctattgct atgttaccta ttttgacggg caatgtcggc atcagcggcg
     4681 gaaatagtgg cgcgcgtgaa tcgacctata ccattacgat agaacgccta ccggtgctgg
     4741 ataatccggt caaaacgtca atctcctgct tcagctggac ggatgctatc gatcatggcc
     4801 cgcaaatgac ggcactccgc gacggtgtcc ggggcaaaga taaactggat gtgcccatta
     4861 agttcatctg gaactacaag gacttgaagc ggacccttag taggcctgag gtcctgcatt
     4921 gagtcaagac cttgtgtcat tctttgtgcc agacttgtgt cattagacgg gatgtctaga
     4981 cggctggtca ggaccctccg gtggcctcgg tctggacttg aggcgggcta tgggggaagt
     5041 ctgctggctt aatgtattag atggtcattc acatttttct gtcagaaacc atcggtccca
     5101 ctgtgagtac aaacctaagc ccccagaagc ccacccacag ccttagacag agtgtcgaat
     5161 acattcacac cgagtcgacc ggagaggacc atatagaggc ccacagagag gaacacgagt
     5221 ttatagacca gagtcaggag ggaccttagg tcttcggaag tcatggacat aaagcccccc
     5281 cctatcttct attgcactac cgcactagac aactagtaca aaacaaaggc ccagaccatt
     5341 ggtccagacc gtagttagtt aatcacagtg acaagccacc acctgaagtc ctgctgatat
     5401 tcagtccaga cttacctctg ctcatggtct tacctccctc ggtctcctga ccttgggtat
     5461 cgactacagc ttgctgagcg tcatctggag gagctacagg aggagctaca ggaggcggga
     5521 ccgaagcccc accttgagtc ccaccaccac ctgtcaggcc cttggcgatg gacctgaagc
     5581 ctttactaat gctcttaccc attggtctta cgcttcggtc ttggtgaact tcaggcgttt
     5641 gactacaggc aggacggcag tagctgagcc agtgcccatc ttgatggtcg tagcagcttc
     5701 agcgacaacg aacactttac cgaggatttc caagtcgtgg gactgagagt cgccatcacg
     5761 tgaaaggacg accgtaatat tctcaccgtc gtgacggaag cagccgaagc tcagggccgt
     5821 gaagcgttta gccagcatga atacaccctt gtcttcaccg ttgtaatagg cgatgtctgt
     5881 ccccagcata tcgacgatag cgtcttcacc ggagaagcct tgcttagcag ccagacggga
     5941 cagtgctttc agttggctct catgagcgat aataccgtag tcagacacct cagaaccgaa
     6001 agaagactct gtgttctcat acagagcgtc catgatagcg tctgccttag cggtcaggct
     6061 accagtcagg acaggaagct ctacggtctt cagcagctta gaggattcca gaacgtcgac
     6121 catgcgagcg ttgatggtac ggaccacatt ctcacagatg ttcatagcgt cctgctgaag
     6181 tgtgccctga gtgaatccgg tggatgactt ggcgtcccag agcttggtct gctcagagat
     6241 gagaatagcc tgctcgattg gttcgaccac gatttcttgt ccagacggaa cggtcacgac
     6301 ttcaggtcgc ttgccaccac ggacaggaac gaccacagag cggtcagcca gagtgtgagc
     6361 aatgattggg gtcggtaagc tgttacgggt cggcaggact tctacaggga acgggatgtt
     6421 ctcttgagcg tctttaagta caccgttcag catagcctta accgtcttag ctaactggag
     6481 ggcatagacc tgcgggtcag cgttagtcgg cagaccttca ttgacctgaa tgttgtcggt
     6541 atcggtgatg cgtactggct caatgccaaa ctggaaagcg tctttaatcg tagtcataat
     6601 aatcaatctc ctaaattagt ttaaatactg tgagtcctca aaactgagga gtccctaaca
     6661 tcgggtcagg gactgtgtgt tttacaagct gtccatccag ttaccgcctg actcaaggtc
     6721 agcgacaata gcggcacggg agcgaccagc ggaatgggta gcactgcgga ggctctcacc
     6781 ataggtccgg cgttcagctt tgacctgttc gcggcgctgt agctctttct gccactctgc
     6841 atcggtcatg ttctcttcac gcatacgctc agcctgaagt cgttgctctt tagcgatacg
     6901 ctcagcttca gctcgtttgt tcatctggcg gcgaccatag gcggtcatca agtcattact
     6961 gaagaagttg ccaatggtca cgccagcttc agccagagct tccagtaact ccattgcctc
     7021 gcggctggta gacggcaggc ctgacaggta gcccggaatg agggtcttga tagcgagcga
     7081 cagcggatag ggctgaccgt ccagactcac agtgttttca taaaggttga tgtcggtgac
     7141 tgacagcttc cagttgttgt tgttgaacat cataggtaat tctcctcttg agagttgaga
     7201 ctatcggtat cgcagacgca gtagcgccta cgggtctccc cgcatcggtc atctcaaaag
     7261 gtttaaaatt aggtttggta tggtcaggac tgcttagcaa aggctcggcg taactgattg
     7321 tctaaatcac ggtccaccag catagctatc agctcgtctc gactgtagta agggcgctca
     7381 gggtcttcga ctccaagtct cagcagagac agcagatgac gtgtgggcag gccacggtaa
     7441 cgaagggtcg ctttgccgat aggtgtacga gggtcaatca tagccatgat tacttacctc
     7501 cactcacagc gaccagcggt cacagcttga tagtctgaat actcagcctg tgagacttca
     7561 cgtccggcga tgtagaggcc tgtgccatga cgctggatga attccttggt ctccggcttg
     7621 atgtcctcag cagacgctac agcagactgc acggtggcgc tggaggtcat agacacgaag
     7681 gctcgggtta ctgccagtcc gtacccaagg tccaaccacc gaccgaactc tatagcggct
     7741 actgggtcag ccagccacga ggtccctaag tgacccatgt taatcttttt aacaaacccg
     7801 ttttctgggt tggttagact tctaccagcc ttggtcctca tgaagcgagc aacctcggta
     7861 ctacggttat cttctacctt accaatctga atgcctaccc ggcgtatctc atccagtcga
     7921 aacaatccgt cctgactggt gatgtcctga ccgaagatat gaatggtgta ttcctgctta
     7981 ttgtcagaag tcataagagg cacctcccgc cagagcttcg gcaaaggact tgaagcgctg
     8041 ttcttggtca gctttcgcct gaaggcgttc ggcatcaggt gggaccagct tatcaaaggt
     8101 gccgttttcg taatcttcag ttaggacctg aaggcggcgg gaccatttgc tctccacctg
     8161 catcatcgcg ttagagatgt g
//
LOCUS       DAJTUP010000151         8167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTUP010000151 DAJTUP010000000
VERSION     DAJTUP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.153"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..294
                     /locus_tag="OO468_003663"
     CDS             <1..294
                     /locus_tag="OO468_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR6260630.1"
                     /translation="RWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALSSTL
                     DSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            266..2176
                     /locus_tag="OO468_003664"
     CDS             266..2176
                     /locus_tag="OO468_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR6260631.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2177..2380
                     /locus_tag="OO468_003665"
     CDS             2177..2380
                     /locus_tag="OO468_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6260632.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2377..3970
                     /locus_tag="OO468_003666"
                     /pseudo
     CDS             2377..3970
                     /locus_tag="OO468_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3960..5378
                     /locus_tag="OO468_003667"
     CDS             3960..5378
                     /locus_tag="OO468_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR6260633.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATDAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPLAAMPEMTVEKARPILAASPQANAGPSLRDQIMAL
                     DEAKGAEAQAEKLAACPGMTVENARAVLAAASGRAEPVSASTTAMFEHFMANHSPAAV
                     QGGVPQTSADGDADVKMLMAMP"
     gene            5415..5762
                     /locus_tag="OO468_003668"
     CDS             5415..5762
                     /locus_tag="OO468_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR6260634.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5820..6848
                     /locus_tag="OO468_003669"
     CDS             5820..6848
                     /locus_tag="OO468_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR6260635.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6900..7286
                     /locus_tag="OO468_003670"
     CDS             6900..7286
                     /locus_tag="OO468_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6260636.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7298..7675
                     /locus_tag="OO468_003671"
     CDS             7298..7675
                     /locus_tag="OO468_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6260637.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7662..>8167
                     /locus_tag="OO468_003672"
     CDS             7662..>8167
                     /locus_tag="OO468_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6260638.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDAR"
BASE COUNT         1887 a         1922 c         2523 g         1835 t
ORIGIN      
        1 agatgggaac tgaccgcaga acaggctgtc gcacagcagt taaaaaatca ggtgtcaaaa
       61 ggaaatctta tcgataccgg attttgtatt tttgccctta gtaagctggc gatggcttta
      121 tccagtacgc ttgactccat tcctttgtcc atgcaacgac agttcccgga tttaacgccg
      181 cgtcatattg atcatctgaa aatccttatt gctaagggag cgaatcagtg cgcgcgtgcg
      241 ggggataaat taccggattt actcgatgaa tatatcagaa caacaactga gtaatatgat
      301 ggtggcagtc actatcgcac tgcagccact ggtcagggta ttgccggtga cggctgtaga
      361 atgggccgat caaaattatt acctgcctaa agaatcgtca tacggtgagg gggaatggaa
      421 aacgctgcag tttcaggtcg ccatcatgaa ctgcatgggc aatgacgaga ttcgtacagt
      481 taacctgatt aaatctgccc gtgttggtta tacaaaaatg gtgcttggtg tgatcgggta
      541 ttttattgag cacaaatccc gcaacactct tctttttcag ccgacggact cagctgctga
      601 agattttatg aagtctcacg tggaagcaac aattcgtgat gttccttgcc tgaaaaaact
      661 ttctccctgg ctgggccgga aacaccggga taatacgctc acactgaagc gtttttcctc
      721 tggtgtgggg ttctggtgtt tgggtggtgc tgctgccaaa aactaccgtg aaaaatctgt
      781 cgatgtggtc tgctatgacg aactctcctc gtttgaaccg gatgtggaaa aagaaggttc
      841 gccaacactg cttggcgata aacgtattga aggttcggta tggccaaaat ccatacgcgg
      901 ttcaacgcca aaaattaaag gctcctgtca gattgaaaaa gctgcgaatg aatctgcgca
      961 tttcatgcgg ttttatgtcc cttgccctca ttgcggggag gagcagtatc tgaagtttgg
     1021 cgatgatgcg acgtcgtttg gcctgaaatg ggagaagggt aaaccggaaa cggtgtatta
     1081 cctgtgtgaa cataatggct gcgtgatccg tcagtctgaa cttgatcaga ccggggggcg
     1141 ctggatttgt gacaataccg ggatgtggac gtgtgacggt ctgacgtttt tcagtgcttc
     1201 gggtaatgaa attccgccgc cgcgctccat cactttccat atatggacgg cgtacagtcc
     1261 gttcaccacc agggtacaga tagtctgtga ctggctggat gcactgaaag atcccaacgg
     1321 cgtgaaaact tttgtgaaca ccacgctggg cgagacctgg gaagaggccg tgggcgaaaa
     1381 actcgatcac caggtactga tggataaggt tgtgccttac acggcaacgg tgcctgtccg
     1441 ggtggtttat ctgacggcgg gcattgactc gcagcgaaac cgttttgaga tgtatgtctg
     1501 ggggtgggct ccgggagagg aagcctttct ggtggataaa atcatcatca tgggacgtcc
     1561 tgatgaggaa gagacgctgt tacgtgtgga tgcggcgatc aataaaaaat accgccatgc
     1621 agacggtacc gaaatgacca tttcccgtgt ctgctgggat atcgggggga tcgatggcga
     1681 aatcgtgtat cagaggtcaa aaaaacacgg tattttccgg gtactgccgg taaaaggtgc
     1741 ctctgtctat ggcaagccgg tgatcaccat gccgaaaacc cgcaatcagc ggggtgtgta
     1801 tctgtgtgag gtggggacgg ataccgcaaa agaaattctc tatgcccgaa tgaaggccga
     1861 tcccacccct gtgaatgagg ccacatcgta tgccatccgc tttcctgata atccggagat
     1921 tttttcgcag acagaggcac aacaactggt ggcggaggaa ctggtggaaa agtgggaaaa
     1981 aggaaagatg cgtctgttgt gggataacaa aaagcggcgt aacgaagcgc tggactgcct
     2041 ggtgtatgcc tatgcggcat tacgtgtgtc cgtgcaacgc tggcaacttg atctggctgt
     2101 gcagttgcat cccgcaagga tgcaactgca caaccgaccc ttgaagaact ggcagcgaag
     2161 ctgtccggag gagtgaatgg ttacagtcgc tgaactgcag gcgctgcgtc aggcgcgcct
     2221 tgatttatta accggtaaac gggttgtgtc ggtacagaaa gacggtcgca ggatcgaata
     2281 tacggcagcg tccctgaatg agcttaaccg tgcgatcaac gatgcggagt cggttctggg
     2341 tacgacccga cgccgccgac cgctgggagt gaggttatga aacgaacgcc tgtcctgatt
     2401 gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acggaggcgg cgcaggattt
     2461 ggcgggcaaa tggcagagtg gttaccaccg gcgcagagtg ccgatgcggc cctgttgcct
     2521 gcattgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg tatcgcggct
     2581 aatgcggtgg ccctgcataa ggatcatatc gtcgggcaca tgtttcttat cagctaccgt
     2641 ctgaactggc gttggctggg gatgcgggag accacggcaa aaagttttgt cggggaggtg
     2701 gaagcagcct ggtcggaata cgcagaagga atgtttggcg agatcgacgt ggaagggaaa
     2761 cgcacgttta cggaatttat ccgtgaaggg gtgggcgtgc atgcctttaa cggcgaaatc
     2821 tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg ttttaaagcc
     2881 gtgagtccga aacgggtgga cacgccagga cacggtatgg ggaaccactt tctgcgggcc
     2941 ggggttgagg tcgatcggta tgggcgggcg attgcgtacc atatctgtga ggatgatttt
     3001 ccgttctctg gtgccggacg ctgggagcgg atcccgcgtg agcttcccac cgggcgtccg
     3061 gccatgctgc atatttttga gccggtggag gacgggcaga cccgtggggc taatcagttt
     3121 tacagcgtaa tggagcggat gaagatgctg gactccctgc aggccacgca gcttcagtcg
     3181 gccattgtga aggcgatgta tgcagccaca atcgaaagtg aacttgatac cgaaaaggca
     3241 tttgaatata tcgcgggggc accgcaaggt cagcaggata atccgcttat taatattctg
     3301 gagaagttca cccgctggta tgacacgaat aacgtgacgc tgggcggtgt taaaattccg
     3361 cacctttttt cctggcgatg cgctgaacct gcagacagcg caggattcag acaatggatt
     3421 ttcggcgctt gaacaggcgc tgctgcggta tattgcagcc ggtcttggcg tttcctacga
     3481 acagttgtcc cgtgattact cgaaggtcag ttactcaagt gcccgcgcct ccgccaatga
     3541 gtcgtggcgc tattttatgg ggcggcgaaa atttattgcg gcccggctgg ccacgcagat
     3601 gttttcctgc tggctggaag aggcgcttct tcgggggatt attcgtccgc cacgggcgcg
     3661 ttttgatttt taccaggcgc gttcagcctg gtcacgggca gagtggatta gctccggaag
     3721 aatggccatt gacgggctca aggaggtcca ggaatcggtg atgcgcattg aggccggact
     3781 gagcacgtat gagaaagaac tggcactgat gggcgaagat tatcaggaca ttttccgcca
     3841 gcaggtcagg gaatccgcag agcgcgaaaa agccggactc tcacgtccgg tgtggatagc
     3901 ccaggcgtat cagcagcaga tagcggagag tcgcaggccg gaagaggaga caacatcacg
     3961 tgagacgtaa tctttcacat attatcgctg cggcattcaa tgagccgctg cttctggagc
     4021 ccgcctatgc gcgggttttc ttttgcgcgc tcgggcgtga gatgggcgca gcaagccttt
     4081 cggtaccgca acagcaggta cagcttgacg ctcccgggat gctggctgaa acggacgagt
     4141 acatggccgg aggcaaacga cctgcccgtg tttaccgggt ggtgaacggt attgctgtac
     4201 tgccggtgac cggcacgctg gtacaccggc tgggtggcat gcggccattt tccggaatga
     4261 ctggctatga cggcattgtc gcctgtcttc agcaggcaat ggcggacact caggtacggg
     4321 gcgtactgct ggacattgac agttctggcg ggcaggccac cggcgcgttt gactgcgctg
     4381 acatgattta ccgtctccgg cagctgaagc ctgtctgggc actgtgcaat gacacggcct
     4441 gttcagccgc catgttactg gcatcggcct gctcccgacg gctggttacc cagacatccc
     4501 gtatcggctc cattggggtg atgatgggcc atgtcagcta tgccggtcat ctggcacagg
     4561 ccggtgtgga tatcacgctg atttacgccg gatcgcacaa ggtggatggc aatcagtttg
     4621 aatccctgcc agcaaaggtt cgccaggaca tgcagcagcg cattgatgcg gcgcgccgga
     4681 tgtttgctga aaaagtggcg atgtataccg gtttatctgt ggatgcggtt atgggaacag
     4741 aggccgccgt ttttgaaggt cagtccggta ttgaggccga actggcggat gaattaatca
     4801 atgcgtcgga cgccatcagc gtgatggctg cgacgctgaa cacacacgat acaggaggca
     4861 ctatgccgca attaactgca acggatgctg ccgcgcagga gaaccagcga gtgatgggga
     4921 tcctgacgtg tcaggaagcg aaaggacgtg aacaactggc cacgatgctg gcagggcaac
     4981 agggtatgag cgttgagcag gcccgggcga ttctggccgt ggcggcaccg ctggcggcga
     5041 tgccggagat gacggtggaa aaagcccgtc cgattctggc tgcttcaccg caggcgaatg
     5101 ccggaccctc acttcgtgat cagattatgg ccctggatga ggcaaaaggg gcagaggcgc
     5161 aggctgaaaa actggcggcc tgtccgggga tgaccgtgga gaatgcccgg gctgtactgg
     5221 cggcagcctc aggcagggca gaaccggttt ctgcatccac aaccgccatg tttgaacatt
     5281 tcatggcgaa ccattcaccg gcagcggtac agggtggcgt gccacagacg tcagcagacg
     5341 gtgatgcgga cgtgaaaatg ctcatggcca tgccatgaag tcagtgctga ccatcaatat
     5401 gaggttttta caatatggta acgaaaacca tcactgaaca gcgtgcggaa gtacgtattt
     5461 ttgccggtaa tgatccggcc cataccgcca caggctgcag cgggatttcc tcggcaacac
     5521 cggctctgac gcccctgatg ctggatgagg ataccgggaa actggtggtc tgggacggac
     5581 agaaggcagg ccatgtggtt ggcatactgg tactgccgct tgaaggtaca gagacggtgc
     5641 tgacgtatta caagtcgggg acctttgcga cggaggccat ccactggcct gagagagtgg
     5701 atacacacaa aaaggcgaat gcctttgccg gcagtgccct gagtcacgca gcgctgccgt
     5761 aacattttat caggtcactg cggtagcctg actgatttct gaatgaaagg aactgattta
     5821 tgggattgtt tacgacccgc cagttactcg gttataccga acaaaaagtt aaatttcgtg
     5881 cgctgtttct ggagctgttt ttccgccgta cggtgaattt ccataccgaa gaggtaatgc
     5941 tggacaaaat taccggaaaa acgccggtgg cggcctatgt ctccccggtt gttgaaggaa
     6001 aggtgttgcg tcaccgtggc ggtgaaactc gtgtactgcg cccgggctac gttaagccga
     6061 aacacgaatt taattaccag caggcggtgg agcgtcttcc cggtgaagat ccggcccagc
     6121 tgaacggccc ggcttaccgt cgcctgcgta tcatcaccga taacctcaaa caggaagagc
     6181 acgccattgt ccaggtggaa gagatgcagg cggtgaatgc cgtgctgtat ggcaaataca
     6241 ccatggaagg agagcagttt gaaactgttg aggtcgattt tggccggtcg gtgggaaata
     6301 acattacgca ggcaggaggg acggagtgga gcgctcagga ccgcgatacc tttgatccga
     6361 cgcatgatct ggatgcgtac tgtgattttg cttccggcac catcaatatt gccattatgg
     6421 atggtaaggt ctggcgtctg ctgaatggct ttaagctgtt ccgcgaaaaa ctggataccc
     6481 gtcgtggctc aaattcacag ctggaaacgg cagtgaaaga cctgggggcg gtggtgtcct
     6541 tcaaagggta ttatggtgat ctggcaatcg tggtggcaaa aacgtcctat gtggcagagg
     6601 acggtaccga aaaacgttat ctgccggacg gcacactggt actgggaaat acggccgcag
     6661 agggtattcg ttgctacggt gccattcagg atgcgcaggc attgtctgaa ggcgtggtgg
     6721 cgtcttcccg ttatccgaaa cactggctga ccgtgggtga tccggcgcgt gaattcacca
     6781 tgacgcagtc cgcgccgctg atggtgctgc cggatccgga tgagtttgtg gtggtgcagg
     6841 tgaaataatc cgtgagcggg agctaaatgc tcccgttttt cattcaatca ggagcgggta
     6901 tggctacgaa agaagaaaac ctgaaacgtc tttgtgagct ggctgagagg ctggggcgtg
     6961 aaccggatgt ctccggtagt gcggcagata ttgcgcagcg agtggcggaa ctggaggaag
     7021 agcttggaga tgcgggggag gtcgcagagc cggatacgta ctcaccatcg aaagatgatc
     7081 cgaccgcgca taaaggcgag tcagttcctg aacagccagg cattgttacc ggagacgaaa
     7141 cggggcaggt tacggtggtg gcactggcga cactgcatac aaaatgtaat ggttcagtga
     7201 tatttgtttc accgggaacg tcgtttcgtg tttctgctga ggtggctgcc agcatggcgg
     7261 caggtggtct ggcaaaaagg cagtaacagg agatgtcgtg gacggtttcg ataatctgtt
     7321 tgatgctgcg cttgccggtg tcaatgaagt cattctccgt gatatgggga tcagcgccgt
     7381 cattacgtcc ggagaacttg aggggacaca cctgacaggg gtttttgatg atcccgaaag
     7441 tatttctttt gttgccgggg ggatccggct tgaggattcc tcaccgtgcc tgtttgtgaa
     7501 aacggcagat atatcgcagt tacgccgtca ggatacgtta actatcgggg atgattcttt
     7561 ctttgtggat cgtattactc cggatgacgg gggatgctgt tatatccggt taaggcgggg
     7621 cagtcctgcc ccacagaata atgccagaat gaggcgatat gatgaaggga cttgagaatg
     7681 ccatccgcaa tctgaacagc ctcgataccc gcatggtgcc acagaccagc gcatgggcgg
     7741 taaaccgtgt ggcggcaaag attgtttctg tcgccacacg gcaggttgcg cagaataccg
     7801 ttgccgggga taaccaggtg aaaggtattc ccctgaaaac ggtcagggag cgtgtcaggc
     7861 tgcttaaagc cagcccctca ggaaaaatgt atgcccgcat gcgtgttaac cggggtaacc
     7921 tgcccgccat taagctgggt accgcacagg tcagactggc ccgctcccgg catggcagca
     7981 actcacgtca tcgtggcagc gtactgaaag tggggaaata ccttttccgg gatgcgttta
     8041 ttcagcaact ggcgaatggt cgctggcatg tgatgcggcg tattgacggc aaaaatcgtt
     8101 accccattga tgtggtgaaa atcccgatgt ccggaccgct gacacaggca tttgaagatg
     8161 cccgcga
//
LOCUS       DAJTUP010000152         8160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTUP010000152 DAJTUP010000000
VERSION     DAJTUP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.154"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(168..788)
                     /gene="entD"
                     /locus_tag="OO468_003673"
     CDS             complement(168..788)
                     /gene="entD"
                     /locus_tag="OO468_003673"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR6260639.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(963..3203)
                     /gene="fepA"
                     /locus_tag="OO468_003674"
     CDS             complement(963..3203)
                     /gene="fepA"
                     /locus_tag="OO468_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR6260640.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3446..4648
                     /gene="fes"
                     /locus_tag="OO468_003675"
     CDS             3446..4648
                     /gene="fes"
                     /locus_tag="OO468_003675"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR6260641.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4651..4869
                     /gene="ybdZ"
                     /locus_tag="OO468_003676"
     CDS             4651..4869
                     /gene="ybdZ"
                     /locus_tag="OO468_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR6260642.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4866..>8160
                     /locus_tag="OO468_003677"
     CDS             4866..>8160
                     /locus_tag="OO468_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit F"
                     /protein_id="HCR6260643.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSI"
BASE COUNT         1753 a         2186 c         2239 g         1982 t
ORIGIN      
        1 taatattgag tttaaggctg ttcttgctta cgaaccgaag aagtagccgt tgtgcgggga
       61 gtaatccccg catatccggt tgtcaggtca gagtggtaag gcacctgctt tacactttcg
      121 cccgcagtca gtgatggctg cgggcgaatc gtaccagatg ttgtcaatta atcgtgttgg
      181 cacagagtta tgactatttt ttcttttatc tgccagtgca cagcaaacat ctcattctca
      241 cgatgaatga tgacctgctg tttattccag ctaattatct gatagtccag aaaacctgca
      301 tcagtttgga tctcacttgc cttaaatgcg ctctctttgg cggaaaatgc aagtgtcagc
      361 gccagagaaa aggctaaacc gcagtctgcg agtcgctcgt gttctgctgg tgtaataatg
      421 ttgtctgtca attcggtcgc tgtttgcgca gaaaaaattt cttcgatatc aacgccaatc
      481 ggttgacggg aaaccacggc caatgccgta gccccgcagt ggctaatact gccgtatacc
      541 tccgcaggcc acaccggttg gcgtagctcg ccgattgcgg gcacacattt atagccatat
      601 tcccgcaagg cataaacagc agcgatccgt ccggctaaat gctctgtttt acgtttacgt
      661 ccagcgtgtt gcagttgtgc gtagtgcggc agccagagta aatcctgctc acaaaaattc
      721 gccggatcga actcaacaaa atgcagcgta tgtccggcaa aggggaggga ggtatgcgta
      781 gttttcatat cgaccatatt cgagactgat gacaaacgca aaactgcctg atgcgctacg
      841 cttatcaggc ctacgtgatc ctgcaatata ttgaattggc aagatctttg taggccggat
      901 aaggcgttca cgccgcatcc ggcgtgaacg acgcgcactt tgtcaacaat ctgacgttag
      961 catcagaagt gagtattaat gctcatatac cacgtacgtc ccggctcgtt ataggtatac
     1021 gcgccggcac cggcgatata gttggctcct gccagatcgc ccgtggtctg ggcattacct
     1081 gcacgccaca aacgtttgtc gaacaggttg tccacgccgc cggtcagact gacattcttc
     1141 gtcacatccc aggtcgcgct cagacccaca atgctgtaag gactgatttc tttggtttcc
     1201 gggccaaccg ctggctgacc tttatagttg tacttcttcg gctgctgctt gccgtaccag
     1261 gtgaaggtcg tttgcatcga caaatcttcc cgtgcctgcc agctcagcgt tgagttcaac
     1321 gtgtactccg ggatgatcga caaacggtcg cccgtggttt tgttttcact cttcagcata
     1381 taagtgatgt tattggtcca catcaccgtt tcgctaactg gtacgtttaa cgatccttcc
     1441 agaccttcaa ccactgcttt cggcacgtta tcccactgat agagatcagt gccgactgcg
     1501 ttttgcccta cagccacata gcccgcttca atcttattgc gataatcgtt acggaaccag
     1561 gtgacgcccg ccagccagcc gtcgcgtttg aactccagac caatctcttt gttgatgctg
     1621 gtttctactt tcaggtcatc gttaccttgc agatagcagc cgcccgcgct ggcatagcag
     1681 ccctgacctt tactgtagag aatgtagttc gggttagtct ggtataggct cggcgcttta
     1741 taggcgcggg caatgcctat tttcagcgtg aagtcatcgc ctaaaccttg cgatatgttc
     1801 agcgccgggc tccagttatt gccgacaata ctgtgatgat cgaaacgcag ccccggcgtt
     1861 acgatggtgc tgtcagtcag ctccatgttg ttttcggcaa acagcgagaa aatttctgct
     1921 tttgaatacg ggctacggtc ggtggcactc acgccatcaa tagcgccacc ggtattcgtt
     1981 ccggtcagtg cttgggtgtt ggaacttaag tccttcatcc gttgctgatt ccactccgta
     2041 cccagcgtca gcgtctggtt aacgaggaaa tcaatcggca ggttaacttc gctgtgcagc
     2101 atcacgtcat cgagatcgat atcgacgaaa tcctgtgtcg ctttttcgtt aaatttccct
     2161 tcggtaccgc ccgccagacc ttccggaata cgcgagttac gggtgtgttc atactgcacc
     2221 cagttgctgg tggtcacgcc gttatcccag ccaccgttcc aggtcagcgc gtagttctgg
     2281 cgatacaggc ggttggtttc atcgccatat ttcgagcgag tgtaagcgtc agagttggtg
     2341 ttctgggtat cgcccgcata caggttaccc tggcggctgt aacccgcttc cagttccagc
     2401 gattgcaatg gtgcgaagtc ccagcgcacc acgccgttaa tatctttatt gatcacccct
     2461 tcgcgcccgg ctggtaacgt cgtggcatac gttccggcac gcgcggactg atggccctgg
     2521 ttgatatccc acgcgtcagc ctgagttttg tcgaggttgc catacaagcg gaagctgaat
     2581 tcgtcgccca gcggaccggt caggctaaag ttagtgcgtt tggtggcacc ttcctcttta
     2641 tgttctggcg cattgaaata tgcgtcccag gagccgtgcc actcgccgct gccttttttg
     2701 gtaatgatgt taaccacgcc gcccgccgcg ccgttgccat aacgcgcagc tgccggacca
     2761 cgcagaactt caatacgttc aatcatttca ggtggcaccc aggaggtatc accacgggta
     2821 tcgcgctcgc cacgccagcc ctgacgcacc gagttacggc tgcttaccgg cttgccgtca
     2881 atcaaaatca gcgtgttttc cggacccata ccgcgaatat caatctgtcg gttattccca
     2941 cgctgaccac tggtggagtt accggtcagg ttaacgcctg gcatggtacg gatgatctcc
     3001 gacacatcgc gggcaaccgg gtttttgcgg atttcatctg cggtgatggt cgaaacgcca
     3061 ggagcctgca agttctgctc ggcggcggta acgacaatgg tatcgtcatg tgaaacaggt
     3121 gtatcggtcg gctcttgtgc ctgcgctacc ccataaatcc ccagattgac caacaaggcc
     3181 agggaatgaa tcttcttgtt cattgtttta ttcctgcatt tttgccacga attgcaactg
     3241 tcgggcatgg tcgtcatcaa cacgacgcat cccgttaccg cgaaaacctt tgatcctgaa
     3301 agacacgcag tgcagttggt taattaatgt ccgcgcttcc cacggcgcgc cattacgcta
     3361 ttgcaaatgc aaatagttat taataacatt atcaatatat ttctgcaatc aatgaaaaat
     3421 tgcacagtaa acatggggtt atggtgtgac ggcgttaaaa gtaggaagtg agagctggtg
     3481 gcagtcgaaa catggcccgg aatggcagcg tctgaatgac gaaatgtttg aggtcacttt
     3541 ctggtggcgt gatccccaag gttctgaaga atactcgacg ataaagcgcg tatgggtcta
     3601 catcactggt gtgaccgatc atcatcagaa cagccagccc cagtcgatgc agcgaattgc
     3661 aggtactgat gtctggcagt ggacgacaca actcaatgcc aactggcgcg gcagctactg
     3721 ctttattccc accgaacgcg atgacatttt ttctgcacca tcccccgatc gcctcgaatt
     3781 acgtgaaggc tggcgaaaac tattacccca ggcaatagcc gatccgctga atccacaaag
     3841 ctggaaaggc gggctagggc acgctgtttc tgcactcgaa atgccgcaag cgcctctgca
     3901 accgggatgg gattgtccgc aagcgccaga aataccagcc aaagaaatta tctggaaaag
     3961 tgaacggttg aaaaattcac gtcgtgtatg gatttttacc accggcgatg taacagcaga
     4021 agaacgcccg ctggcagttt tgctcgatgg cgaattttgg gcgcaaagta tgcccgtctg
     4081 gcctgtgctg acttcgctga cccatcgtca gcaacttcct cccgccgtgt atgtgttgat
     4141 cgacgctatc gacaccacgc accgcgccca cgaactgccg tgtaatgcgg atttctggct
     4201 ggcagtacag caagagttat tacccctggt gaaagttatt gcccctttta gcgatcgcgc
     4261 cgatcgcacc gtggtcgccg ggcagagttt tggtgggctg tccgcgctgt atgccggact
     4321 gcactggcct gaacgctttg gctgtgtatt aagccagtca ggatcgtact ggtggccgca
     4381 tcggggcggg cagcaagagg gcgtgttact tgaaaagcta aaagctggtg aagttagcgc
     4441 cgaaggtctg cgcattgtgc tggaagcggg tattcgcgag ccgatgatca tgcgggccaa
     4501 tcaggcgctg tatgcgcaat tacaccctat aaaagaatcc attttctggc gtcaggttga
     4561 cggcggacat gatgcgcttt gttggcgcgg tggcttgatg caggggttaa tcgacctctg
     4621 gcaaccactt ttccatgaca ggagttgaat atggcattca gtaatccctt cgatgatcca
     4681 cagggagcgt tttacatttt gcgcaatgcg caggggcaat tcagtctgtg gccgcaacaa
     4741 tgcgccttac cggcaggctg ggacattgtg tgtcagccgc agtcacaaga gtcctgccag
     4801 cagtggcttg aagcccactg gcgtactctg acaccggcga atttcaccca gttgcaggag
     4861 gcacaatgag ccagcattta cctttggtcg ccgcacagcc cggcatctgg atggcagaaa
     4921 aactgtcgga tttaccctcc gcctggagcg tggcgcatta cgttgagtta accggagagg
     4981 ttgatgcgcc attactggcc cgcgcggtgg ttgccggatt agcgcaagca gatactttgc
     5041 ggatgcgttt tacggaagat aacggcgaag tctggcaatg ggtcgatgat gcgctgatat
     5101 tcgaactgcc agaaattatc gacctgcgaa ccaatattga tccgcacggt actgcgcagg
     5161 cattaatgct ggcggatttg caacaagatt tgcgcgtcga tagcggtaaa ccactggtct
     5221 ttcatcaact gatacaggtg gcagataacc gctggtactg gtatcagcgt tatcaccatt
     5281 tactggtcga tggcttcagt tttccggcca ttacccgcca gatcgccaac atttactgcg
     5341 cattgctgcg tggcgaacca acgcctgctt cgccgtttac gcctttcgct gatgtagtgg
     5401 aagagtacca gcaataccgc gaaagcgaag cctggcagcg tgatgcggca ttctgggtgg
     5461 aacagcgtcg ccaactgcca ccgcccgcgt cactttctcc ggcaccttta gcggggcgca
     5521 gcgcctcggc agatattttg cgtctgaaaa tggaatttac cgacggggaa ttccgccagc
     5581 tggctacgca actttcaggt gtgcagcgta ccgatttagc ccttgcgctg acggctttgt
     5641 ggctggggcg attgtgcaat cgcatggact acgccgccgg atttatcttt atgcgtcgac
     5701 tgggctcggc ggcgctgacg gctaccggac ccgtgctcaa cgttttgccg ctgggtattc
     5761 atattgcggc gcaagaaacg ctgccggaac tggcaacccg actggcaacc cgactggcag
     5821 cacaactgaa aaaaatgcgt cgtcatcaac gttacgatgc cgaacaaatt gtccgtgaca
     5881 gcgggcgagc ggcaggtgat gaaccgctgt ttggtccggt actcaatatt aaggtatttg
     5941 attaccaact ggatattcct ggtgttcagg cgcaaaccca taccctggca accggtccgg
     6001 ttaatgacct tgaactggcc ctgttcccgg atgaacacgg tgatttgagt attgagatcc
     6061 tcgccaataa acagcgttac gatgagccaa cgttaatcca gcatgctgaa cgcctgaaaa
     6121 tgctgattgc ccagttcgcc gcggatccgg cgctgttgtg cggcgatgtc gatattatgc
     6181 tgccaggtga gtatgcgcag ctggcgcaga tcaacgccac tcaggttgag attccagaaa
     6241 ccacgctgag cgcgctggtg gcagaacaag cggcaaaaac accggatgct ccggcgctgg
     6301 cagatgcgcg ttacctgttc agctatcggg aaatgcacga gcaggtggtg gcgctggcga
     6361 atctgctgcg tgagcgcggc gttaaaccag gggacagcgt ggcggtggca ctaccgcgct
     6421 cggtcttttt gaccctggca ctccatgcaa tagttgaagc tggcgcggcc tggctaccgc
     6481 tggataccgg ctatccggac gatcgcctga aaatgatgct ggaagatgcg cgtccgtcgc
     6541 tgttaattac caccgacgat caactgccgc gctttagcga tgttcccaat ttaaccaacc
     6601 tttgctataa cgccccgctt accccccagg gcagtgcgcc gctgcaactt tcacaaccgc
     6661 accacacggc ttatatcatc tttacctctg gctccaccgg caggccgaaa ggggtaatgg
     6721 tcgggcagac ggctatcgtc aaccgcctgt tgtggattca aaatcattat ccgctcacag
     6781 gcgaagatgt cgttgcccaa aaaacgccgt gcagttttga tgtctcggtg tgggagtttt
     6841 tctggccgtt tattgccggg gctaaactgg tgatggctga accggaagcg caccgcgacc
     6901 cgctcgctat gcagcaattc tttgccgaat atggcgtaac gaccacgcac tttgtgccgt
     6961 cgatgctggc agcatttgtt gcctcgctga ctccgcaaac cgctcgccag agttgcgtga
     7021 cgttgaaaca ggttttctgt agtggtgagg ccttaccggc tgatttatgc cgcgaatggc
     7081 aacagttaac cggcgcgccg ttgcataacc tgtatggccc gacggaagcg gcggtagatg
     7141 tcagctggta tccggctttt ggcgaggaac tggcacaggt gcgcggcagc agtgtgccga
     7201 ttggttatcc ggtatggaat acgggtctgc gtattcttga tgcgatgatg catccggtgc
     7261 cgccgggtgt ggcgggtgat ctctatctca ccggtattca actggcgcag gggtatcttg
     7321 gacgacccga tctgaccgcc agccgcttta ttgccgatcc ttttgctcct ggtgaacgga
     7381 tgtatcgtac cggagacgtt gcccgctggc tggataacgg cgcggtggag tacctcgggc
     7441 gcagtgacga tcagctaaaa attcgcgggc agcgtatcga actgggcgaa atcgatcgcg
     7501 tgatgcaggc gctgccggat gtcaaacaag ccgttaccca cgcctgtgtg attaaccagg
     7561 cggcagccac tggtggtgat gcgcgtcagt tggtgggcta tctggtatcg caatcgggcc
     7621 tgccgttgga taccagcgca ttgcaggcgc agcttcgcga aacattgccg ccgcatatgg
     7681 tgccagtcgt tctgctgcaa ctgccgcagt tgccacttag cgccaacggc aagctggatc
     7741 gcaaagcctt accgttgcct gaactgaagg cacaagcgcc agggcgtgcg ccgaaagcgg
     7801 gcagtgaaac gattatcgcc gcggcattcg cgtcgttgct gggttgtgac gtgcaggatg
     7861 ccgatgctga tttctttgcg ctgggcggtc attcgctact ggcaatgaaa ctggcagcgc
     7921 agttaagtcg acagtttgct cgtcaggtga cgccggggca ggtgatggtc gcgtcaaccg
     7981 tcgccaaact ggcaacgatt attgatggtg aagaggacag ctcccggcgc atgggattcg
     8041 aaaccattct gccgttgcgt gaaggtaatg gcccgacgct gttttgtttc catccggcgt
     8101 ccggttttgc ctggcagttt agcgtgctct cgcgttatct cgatccacaa tggtcgatta
//
LOCUS       DAJTUP010000153         7938 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTUP010000153 DAJTUP010000000
VERSION     DAJTUP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.155"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..238)
                     /locus_tag="OO468_003678"
     CDS             complement(<1..238)
                     /locus_tag="OO468_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6260644.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPAT"
     gene            complement(325..686)
                     /locus_tag="OO468_003679"
                     /pseudo
     CDS             complement(325..686)
                     /locus_tag="OO468_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1025..1579)
                     /locus_tag="OO468_003680"
                     /pseudo
     CDS             complement(1025..1579)
                     /locus_tag="OO468_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1722..1844
                     /locus_tag="OO468_003681"
                     /pseudo
     CDS             1722..1844
                     /locus_tag="OO468_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2106..2672)
                     /gene="ipgB2"
                     /locus_tag="OO468_003682"
     CDS             complement(2106..2672)
                     /gene="ipgB2"
                     /locus_tag="OO468_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR6260645.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2954..3268)
                     /locus_tag="OO468_003683"
     CDS             complement(2954..3268)
                     /locus_tag="OO468_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260646.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3346..3456)
                     /locus_tag="OO468_003684"
                     /pseudo
     CDS             complement(3346..3456)
                     /locus_tag="OO468_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3780..4457)
                     /gene="ospD1"
                     /locus_tag="OO468_003685"
     CDS             complement(3780..4457)
                     /gene="ospD1"
                     /locus_tag="OO468_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR6260647.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4829..4966)
                     /locus_tag="OO468_003686"
                     /pseudo
     CDS             complement(4829..4966)
                     /locus_tag="OO468_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(5048..5346)
                     /locus_tag="OO468_003687"
                     /pseudo
     CDS             complement(5048..5346)
                     /locus_tag="OO468_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5935..6094)
                     /locus_tag="OO468_003688"
                     /pseudo
     CDS             complement(5935..6094)
                     /locus_tag="OO468_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6098..7327)
                     /locus_tag="OO468_003689"
                     /pseudo
     CDS             complement(6098..7327)
                     /locus_tag="OO468_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7347..7694)
                     /gene="tnpB"
                     /locus_tag="OO468_003690"
     CDS             complement(7347..7694)
                     /gene="tnpB"
                     /locus_tag="OO468_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6260648.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7694..7938)
                     /locus_tag="OO468_003691"
                     /pseudo
     CDS             complement(7694..7938)
                     /locus_tag="OO468_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         2034 a         1850 c         1737 g         2317 t
ORIGIN      
        1 ccgttgccgg gattttgtaa ggatgagcat cggcgatgcg ttgacgaata taatcccggt
       61 attcatccag gagtgaagca acagcaggtc gcggcgtata ttttggcggc tcagattttg
      121 cctgcaaata acgtttaacg gtattgcggg agagccccag ttctctggca atcgcccggc
      181 tactcattcc ctgcttgtgc aggattttaa tttccataac tgtctcaaaa gtgaccataa
      241 gctctcctga atcaggagag cagattaccc cctggatctg atttcaggcg ttgggtgtgg
      301 atcactattg caccgttcgt gacagtgttg ttgcgccttg ctgccaacga agctccactg
      361 cccatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
      421 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagcgtacgg
      481 ccagtatcgc gaactccggc accgttgaaa gccatttcag tgatttggtt tttaactccc
      541 ggtttacggg cctcgtaaca gtaagtgagc tgaaacaccc ggtgacagtc acggcaacgg
      601 aatcgatcat gtccttttgg gttctgcccg tgacggtaaa cctgaacgga ctggcaacgg
      661 gggcaatgaa agttaactgt gaccatgaaa gaacctaaaa gcgggtatta taacccctaa
      721 ttcaactaat tggaggcatt gtcattttta atctatggat acacttaaaa atcctctcac
      781 cttacgttaa aaaccttctc atgtttagag ttttttatta tgtttatcat aatgatatga
      841 cgatctgagt ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac agtgcctgtg
      901 gataactttg cgtaaatccg gaggacagat cactctgaag caagacaaca gcatctgaaa
      961 acaatatgct cacgtttcaa ccacagggcg taagtgatcc tacccacgta atatggacac
     1021 aggcctaagc gaggttctgg ttttcaaatt gttccggact gaggccgcca caccaactgt
     1081 gccgtcgcca gcgattgtaa tcacattcga tataattaaa cactgttgcc ctcattattt
     1141 cccggctgat aaagtgttct ccgtggagac attccacttt cagcgaatga aagaagcttt
     1201 ccacgcaggc attatcgtag cagcagccct ttgcgctcat actgccacgc agattatgcc
     1261 acttcagcag cgcctgataa tctgctgaac agtactgact tcctcggtcc gagtgaacga
     1321 taacgttccg gggtctctta cgccgccaca gcgccatctg cagggcatcg caggccagtt
     1381 gcgccgtcag gcgtggtgac atggaccagc caataacggc acgtgaccac aggtcaatga
     1441 ccacagccag atacagccag ccttcatctg tacgtaagta cgtgatgtct cccgcccact
     1501 tctggttcgg accactggcg taaaaatcct gctccagcag attttctgac acaggcaggc
     1561 catgtgcgcg gtaactgacc gtattgctgg gtaagatcaa tttgctcact ggtatttact
     1621 acgtgcatcg aatgagtgat caagagccgt atgacgcgag agtgtcacgt acggttccgt
     1681 aagcagccgg agagtgaaac tctctccggc tgactcgact cgcaggttcc gacagcggtg
     1741 acgaacatgc agcggtgttg ttctcgctga tcggcacatg ccgtctgaac aatgtggagc
     1801 cagaaaaatg gctgcgttac gttattgaac atatccagga ctgaccggca aaccgggtac
     1861 gcgatctgtt gccttggaaa gttgatctga cttctcagta aatatcaata tgattctgac
     1921 gagccgctta cctttgagca gagaaagggg agagggatcc cacatccatg agctaggaaa
     1981 atacaaaaac tggctataca acaattcaat atattaggac ttacttgcga ggagaaatat
     2041 agcaccgctc aggcaattcc tgggtattta cttttcttag tttctttact cgggatgttc
     2101 tgtcatcaga aaggcgattc taaatttgta atatagtcac tcatattccc acgtaatttc
     2161 tttagatcaa tatattgcac atttttctca aaaaactgat cggaaataag atttgcaaca
     2221 ttgcttgttg tatcattttt aacgcttcta ttcatccctt cacaaagttc gtccattttc
     2281 tttttgaaat aactgcttcc tgatacaaga tgatttatgg aactttgtgc agcattgatg
     2341 tctagagaaa tgcgataatc tttctcaacc tgtttaaata tctgtttacg gagattatcg
     2401 ccgatttttc ggctgtgttc tgcacaaaac ttatcaatac attggtttac cacccgggat
     2461 acgtagactt ttttttcatc atttttccaa tctgtaatat tttcaccgat ttgttctgaa
     2521 acagagctca aaatactagt atttattttg gttgatgata tttttactat ccttttcccc
     2581 attgtacagc ggataatctc atccaccttt cctgaaaaat atcttttgtg agataaactg
     2641 attccaaaat tattaaaaga tgttccaagc attataatca ccttgtcatg taaaatagta
     2701 acattgtcat tattttagct gattcaaaca attctgtaaa tgctttgagt tctttactcc
     2761 cttggctcaa tgatggaccg tctggaatcc cagagactaa actctctgag aaagagagta
     2821 tgaaaaaact tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat aaccggcatc
     2881 attcataatg atgccctgtt agttgtagtt atgaagattt acgtcattaa aattgctgtt
     2941 cgtagtgtca gtcctatggt ctggcatcag ctgaggatcg tagccgatac atcactggct
     3001 tcacgacatt cgtgttgagg ctattcatga aaactccacg ctgaaaacgc cgttttgtat
     3061 aaacggtcac ggtatgcccg gagccactgt tgctgataaa ttcgataaaa ccttggcgtt
     3121 tcttgaagct atcgttaatg cggataatga aacaacgatt ggtgagattc gctcttttgc
     3181 cgacgccctg gacgctgtcc ggttcaatcg caacaaaatc aaccggcagc tgagcaaacc
     3241 caaccttgcc tctctggcgc tggagcatga agtgatttgg ctgggacgta gccgctgaca
     3301 gcttctataa aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg
     3361 gttttcaaat tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta
     3421 atcgcacttg atataattaa acactgtata tttcatttgt ggagtctcca gtctgagagg
     3481 gctttatgca ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc
     3541 ctgtatttcc cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta
     3601 catttattgt gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg
     3661 ttctatgggt agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag
     3721 atcacacaat tcggggagag atccttctaa gtgcaattct ttgcagctat ttagcacact
     3781 taaaacaact cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta
     3841 agtctgtctg ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc
     3901 atcatagcct cgatattccc tttccctgcg gccagaaata atcctggtaa cccctcatta
     3961 tctcttacat ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa
     4021 tctccataag cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat
     4081 tccccagcat tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct
     4141 gccaacagcg attcaccgta tttacgcgct gcatcggcat ttccatgcca aatcaatgaa
     4201 aacaaattat ggcggttata ctccttatta ttctttgtcg gaatggatat gttctgtatt
     4261 ttggccaatt ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg
     4321 gcgtgggata tagcggtatt tgtcacgtta atgatattat ttttcattcc tttattttca
     4381 ttagcagtat tgctgcctat tattaagtgc atatttttgt tgtttgctgg atgtaatcca
     4441 tagttattta ttgacatttt tgttcatcct ttttgctaaa aaaaaacaat aacctatttt
     4501 tgtaatgtaa tagatattag tctattgcta atctaaaaaa accattgcca cattgaatgg
     4561 ctatattcac caataaattc tttcgatttc gtatattgac atatcgtaga tatatcttat
     4621 ccctaagatt caataatcga gtccgtttac cattctgtgc aagcgccttc actcagaagt
     4681 gaccgtcaag gcgatcacca aacttgataa taaagcagct cattgccata cgccagtcct
     4741 taaggggcgt tgtccatttc tgtgatgctg cctagattta actgccagct ccggcttatc
     4801 tacatctaaa tatgtgagcc taataccgct acactttgtc cagactatgt ttttctccgg
     4861 gaagtgggct aaccgtctcc ataaagtgac gaactttttt caacagtggc catattgaac
     4921 ggcactgatg attacgggtt attgtctcat gaagagccgg ccacatcact gacagcagtg
     4981 acttcaggcc ttcagcacca gacagcctga accagttaat ccatcgcccg acaaatttag
     5041 taatctaggg ctggtttttc ttcagctgtt gcggttcgaa agtgccatta cgatcacgca
     5101 gagtacggat ttccagaggt tcatctccag tagtgataat cttcgttaag caaccattgc
     5161 gggaattgct gccagatcta agctgatttt tctcgcaccc aagatgatga gtcatattag
     5221 cattgagtac ggtttcattt tcagcatcca atcaaactga ctgagatctt caggattttt
     5281 gagacttttg gctagttcat tagctaaagc ccgcaactta ttagatgtca actgtttttc
     5341 attcataaat tcaccttcat ttgatatcaa attaaatagt tcaacatcag agaattatac
     5401 aaacttatgt acaggctcct atgatcttcg aatcctgaag attatagtga aaataagttt
     5461 tcacgttgaa atcccacata gttagtatat gataaataat catggatgac ctgattaacc
     5521 tcttggggta gtgcttttat agaagaataa atattctact aatttaatac atcacctaaa
     5581 atagtattgt tttttatggt tggttgaagg tcgtggtgat gatgttagtc cttttgagaa
     5641 tattccagtg ccagatctta cttataataa aggggcttgg cagtttggat aaactattgt
     5701 tcttattcca ctaatacaaa ctttttgtaa agtatattaa atatacttca tttcatactg
     5761 aaatccccgg aagtattcaa tcaaacaatt acaccataaa aggaagaggg ggccctaatt
     5821 aagttgtcaa gcatgtttat gatacctgca gggtataaat tttctggctg caagcagaca
     5881 catataaaac gtctttattg atcctaccca cataatatgg acacagtcgt aagtgaggct
     5941 ctggttttca aattgttccg gactgagacc accacaccaa cggtgccgtc gccagcgatt
     6001 gtaatcgcac tcgatataac taaacactgt tgcccgcact atttcccgat ggataatagt
     6061 gttctccgtg gagacattcc attttcagcg aatgaatttt catctgttgc tgtatccagt
     6121 catacagtga ctgcatcagt ggcgcggctc tggcttttct tgccgccaga cgctgttctg
     6181 ctgaacatcc ccggacttct gcctcgatgg catacagttc accgatacgc tgcagggctt
     6241 ccgtggtgat gtcggttggc actcttgcat gcacatcgtg gatttttctc cggacatgag
     6301 ccatacatgc ggcttccgtt attctgccgg attcgtacaa cgcccggtaa ccaccgtaag
     6361 catcggcctg aagcactccg ctgtaaccgg ccagatgatt ctgtggatgg atacctttcc
     6421 ggtccggtga gtacgcgaac cagaccgccg gaggcatttc cgaaccggcg ttacggtcat
     6481 cacgaacgta gacccacagc cgggcagtcc ttgttttacc gctgcccggc tcctggaccg
     6541 gaacggggat atcatcggca tggactttac cgggcatcag cacatactgg cgcaggatgt
     6601 catacagcgg ttccagcagt tcagcaacag cacctgtcca gcgccccagt gtggcacggc
     6661 tcagctccac gccctgacga cggtatattt ctgactggcg gtataacggc agatagtctg
     6721 catattttcc agcaacaaca tgggccagaa gccccgctcc ggcataactg cgtgcaatgg
     6781 gttttgaagg caccggtgcc tgcacgatat ggtcacaccg gcaacaggcc agtttcggac
     6841 gttgtgtttc gataacctta aaggcgctgc tgataagctc cagctgctct gacacatcac
     6901 atcccagaga actgagttcg ccaccacagg ccgggcagca ttcctcttcc ggacggatga
     6961 cccgggtttc acggggaagt gaggctggta acggtttacg ggctgaagac tggcgcaggg
     7021 gagatggcag taccgggtca tattgctcac ccagcgtttc cgccatttct tcctgaagtg
     7081 cgctgattcg ctcctgtgct tccagtatct gccgttcggt ttttgcgcga agtttttctg
     7141 agcttttacc gaactgcatg cgctgcagtt tcgcaaccag cgccttcagc cggttgattt
     7201 cggaagcata agccgccacc cgctgtgaga gcaggcggtt gtatttcgcc atctggcgga
     7261 tggtgtcctg ttgcgtctgc aacagtgccc gcaggcgggc attctcatga gcaagtgagg
     7321 tgtccatatc ctcactttac aacgggttat atgcggatgc cagcgcgttc cgttcgtttc
     7381 gggtgtttcc agtcgatgcc ctcaagaagc atggataact gggccggagt aaggtgcacc
     7441 ttgccgtcac gggtgaccgg ccagacgaag cggccccgct ccaggcgtct ggtaaagagg
     7501 cacagtccgt cactgtcagc ccacaacact tttatctggt caccccggcg tccgcggaag
     7561 atgaacagat gcccggagaa cgggtcatcc ttcaggacgt tctgaacttt tgagaccagg
     7621 ccgttaaagc catttcgcat atcggtgata cctgcaacca gccagatacg cgaacctgca
     7681 gggaaagata tcatcagtgg ctgctccctt ttatttcgcg gataagtatc tgtaataacg
     7741 ccggcgtcag tttaccttta agcctgagag ttccggccgg cagaaccagc tcacagcaca
     7801 gactgtcgga cggtgtattt atctgctctg gttcctgtgc cggagccggg attttattat
     7861 ccggctccgg cgttaacgtc acgggaagca gtgcaggcat atttttccgg aaggcagcag
     7921 gccacctttc cggtattg
//
LOCUS       DAJTUP010000154         7910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTUP010000154 DAJTUP010000000
VERSION     DAJTUP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.156"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(123..953)
                     /locus_tag="OO468_003692"
     CDS             complement(123..953)
                     /locus_tag="OO468_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6260649.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO468_003693"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO468_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6260650.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO468_003694"
     CDS             2379..2729
                     /locus_tag="OO468_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6260651.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO468_003695"
     CDS             2855..3415
                     /locus_tag="OO468_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6260652.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO468_003696"
     CDS             3418..6384
                     /locus_tag="OO468_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6260653.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO468_003697"
     CDS             6451..6828
                     /locus_tag="OO468_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6260654.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO468_003698"
     CDS             complement(7029..7688)
                     /locus_tag="OO468_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6260655.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2242 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
//
LOCUS       DAJTUP010000155         7605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTUP010000155 DAJTUP010000000
VERSION     DAJTUP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.157"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..486)
                     /gene="ospZ"
                     /locus_tag="OO468_003699"
     CDS             complement(<1..486)
                     /gene="ospZ"
                     /locus_tag="OO468_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR6260656.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRAEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFSKENSDFLYIIVVFRNDSSQGELR
                     AN"
     gene            542..694
                     /locus_tag="OO468_003700"
                     /pseudo
     CDS             542..694
                     /locus_tag="OO468_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2075..3022
                     /gene="icsP"
                     /locus_tag="OO468_003701"
     CDS             2075..3022
                     /gene="icsP"
                     /locus_tag="OO468_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR6260657.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3584..3956)
                     /locus_tag="OO468_003702"
                     /pseudo
     CDS             complement(3584..3956)
                     /locus_tag="OO468_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4200..4319
                     /locus_tag="OO468_003703"
                     /pseudo
     CDS             4200..4319
                     /locus_tag="OO468_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4569..5435
                     /gene="ospB"
                     /locus_tag="OO468_003704"
     CDS             4569..5435
                     /gene="ospB"
                     /locus_tag="OO468_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR6260658.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5764..6504
                     /gene="phoN2"
                     /locus_tag="OO468_003705"
     CDS             5764..6504
                     /gene="phoN2"
                     /locus_tag="OO468_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR6260659.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(6864..>7605)
                     /gene="ospC4"
                     /locus_tag="OO468_003706"
     CDS             complement(6864..>7605)
                     /gene="ospC4"
                     /locus_tag="OO468_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6260660.1"
                     /translation="IPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKD
                     MRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNF
                     KKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFT
                     KQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDT
                     MLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2386 a         1427 c         1437 g         2355 t
ORIGIN      
        1 atttgcccgt agttctcctt gggaagaatc atttctgaat acaacaatta tatacaaaaa
       61 atcactattt tctttagaga agatcttaca actaatctct gggagctcac ataatgcatg
      121 taagtcagga tggtacaaac tatccacaga aaattgcccc ataggacgtt ttccagatat
      181 aactgtcaac cagcttcctt ccggtgtact ggcttcggct ctggaaatag gctgaaaatc
      241 ttccggacat ggagaataca ttgtcttcat ataattaagt cgtctggatt tctcattaat
      301 aaactcctct atttttaatt gtgccatact tctggttata ccagagatat acttcttgtc
      361 atatatgttt ggtgattcat taaagtaacc atgttcaact ctaggataaa tatttctcac
      421 tatatattca gtatttgcag tatttattgg aaatacgttc ttaatattct tgatgggact
      481 aatcatttta atctctatac tcaaatataa acattaccat gaacaatata attgttccta
      541 agtggaatgt ctccacggag aacactttat ctatcgggaa atagtgcggg caacagtgtt
      601 taattatatc gagtgcaatt acaatcgctg gcgacggcac cgttggtgtg gcggcctcag
      661 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
      721 ctcacagagc ctcacttacg actgcgtcca tattatgtgg gtaggatcaa taaagacgtt
      781 ttatatgtgt ctgcttgcag ccagaaaatt tataccctgc aggtatcata aacatgcttg
      841 ataacttaat tggggctccc tcttcctttt atggtgtaat tgtttgattg aatacttccg
      901 gggatttcag tatgaaatga agtatattta atatacttta caaaaagttt gtattagtgg
      961 aataaggaca atagcttatc caaactgcca agcctcttta ttataagtaa gatctggcac
     1021 tggaatattc tcaaaaggac taacatcatc accacgacct tcaaccaacc attttattgc
     1081 atttaaatcc cagaatagtt cagtagtggt caatttggga aacgcacaat attgtacggt
     1141 tacgttatcg caatagagtg aactctcgcc ctatacggcg ttttcaagga ttagtcctta
     1201 atcggacaac cacattaaag cataataaat aattaagctt taataatata ctatagatac
     1261 agttctatta taaaaaatac tattttaggt gatgcattaa attagtagaa tatttattct
     1321 tttataaaag caccaatgaa ttgtccaagt aaacatgaga taaaatgtgt agaggataac
     1381 tgagtttggt cactttaact gtattagtcg cagtctgact ctcgacttta aaaggatggg
     1441 aattacctgt actgatagtg agaaagcttt ccattgaaat aagtcaaata aaagttgatg
     1501 gcttcagtgc taaagtgagg tgcccgctag ccgtattcac tcgaatcgga attcaatgta
     1561 ttgcttctgc catttccata atatttcacg ggaaaaacaa aatctgaaaa actatactta
     1621 agtgttgtat tgagtcagtt gagatacaaa tacatattta tcagcacacg ttacgtcaaa
     1681 tacaggaggt tgtcatgttg gtactgaaag gcacgttggc tattgcagcc gtgatgacaa
     1741 tttattgtct tatcgttgtt cttatggaac gcttttctga ttgatttcat attggcgaga
     1801 gaacaagaag gtgagtagaa ttgtcgcgga atcctgaaac tatcagcctc aggtatgaac
     1861 accaaaaggt aggtagagaa atctatggcc cccgttactt tattcgcaca atgaggtatt
     1921 tttttatttt tacaaaagtt caataaatat tataataaaa gatatgttct tggtaacata
     1981 aacaaatatt taattaaaat aatgcttggc attgacgttt atcttataaa gtaagaagat
     2041 catggacatt tcaaccaaaa aagtagagtt ctcgatgaaa ttaaaattct ttgtacttgc
     2101 actttgtgta cctgcgatct ttactacaca tgctaccact aactatccac ttttcatacc
     2161 ggacaacatc agtacggata ttagtctagg atctctgagt ggcaaaacaa aagaacgcgt
     2221 ttatcatccc aaggaagggg ggagaaaaat tagtcaactg gactggaaat acagtaatgc
     2281 gactattgtt agaggtggca tcgattggaa gctaattcca aaagtgtctt tcggagtttc
     2341 cggttggact actttaggta accagaaagc aagcatggtt gataaagact ggaacaattc
     2401 caatacccct caggtatgga ccgaccagag ctggcatccc aatacgcatc tccgtgatgc
     2461 taacgaattc gagctgaatc ttaaaggttg gttattaaat aatttggatt atcgacttgg
     2521 actaatagca ggttaccaag agagtcgtta cagttttaat gcaatgggag ggagttatat
     2581 ttatagtgaa aacgggggga gcaggaataa aaaaggggca catcctagtg gtgaaagaac
     2641 aataggttac aaacagctct tcaaaatacc ttatattgga ttaactgcta attaccgcca
     2701 tgagaatttt gagtttggag cagaactgaa atatagtggt tgggttcttt catctgatac
     2761 agataaacac tatcagactg agacaatttt taaagatgaa ataaaaaacc aaaattactg
     2821 ctctgttgct gcgaatattg gatactatgt cacccccagt gcaaaatttt atatagaagg
     2881 ctccagaaat tacatttcta ataaaaaagg tgatacatct ctttatgagc aaagtaccaa
     2941 tatatctggc accattaaaa atagtgcaag tattgaatat attggttttc tcacttccgc
     3001 aggtataaag tatatttttt gaattggttc accgagttga ttacgttttc atggctaaaa
     3061 attgccgccc attgtgagtc atattcgccc tgactttcca gaaccatacg aactgcccgt
     3121 tgacggactt cgggggaaaa acgtgtattt ttagtcattc tgtttacctc tttctcaggg
     3181 cgtttagtct ccaggatttc cggggcggtt caaaaaactc ttcgttgatt actgtggact
     3241 tactgttggc attaccgacc ctgagaccgg agaaataaga accgcttaga tcatcgtggc
     3301 tgttctcggg gtatcaagtt acacatgggc agaggctacc caaggtctac tattatcgcc
     3361 tgaaagcgct gatggaacag tgctatcagg gacatgctga tgaaaatacc taaataatca
     3421 gccagcactc tatctttcca aatccacagc atagcaaaga gagcaaaaga gcctgtaaat
     3481 tcagaaatta aggaaacgcc tcttggtgta cataatctat cttatgttaa atgaaatcta
     3541 atttcttgat tatatgctgg atcgttcgtg ggtggctttg cccctatatt tccagacacc
     3601 tgttatcatt ggcccgctgc cgcagatatt cccgtggcga gcgataatcc agcgcactat
     3661 gcggatgcca ttcgttataa tgctcgaatg cctctgcaag gttctttgct gccgttaacc
     3721 cgtcaggttt gggcatgata ctgatgtagt cacactttat tgttttcacg aagctctttg
     3781 ctattctgtt actctccgga ctccgcaccg ccgtgttctt cggttcaaca aagtcccaac
     3841 atccgggtga actggcgtgt ttcattagct cggtagcatg aaccattatc cgtcagtcac
     3901 ttcactggag acgccggaag ctcgttgccg aagcggtgtt ccaccgctcc cagcatgtcg
     3961 ccaacgggag agcatctacg gtctgattga actgtttatt caaattgtcc accgtctgtc
     4021 agtacgctct gaacgtcggc tggttaaaac actgctcgct gattttcaga gggttcacgg
     4081 taaaacagca cttttattcc ggattgcgga agcggctctg aataatccgg atgggctggt
     4141 taaagaggtt gtgtaccatc tgcatattgt tgaaccagat cgcagcggta agagaagttc
     4201 atcatacttg gcgcagttac gcgatgtttc tgctagaggt gatgccgtaa agaacggcag
     4261 aacattaccg gaacaagata gcggtctacc tgcactggta tcagacccgg ggcttccccg
     4321 gatgatctcc acggtacttt aggtactgtg tctatattat gtaggtagga tcaattaact
     4381 ttgtagttga aattctttca ttgaaataat gaataaaaaa gacaaaccgc attaaacaat
     4441 gccagaatat aattgcattt ttgttccgtt tttttaaatc atagtataca aagaagcttg
     4501 catactatat gaaaatataa caccctttac tatgatgttt ataaaacaat atatggagtc
     4561 atgtaggtat gaatttagat ggtgttagac catactgtag aatagtcaat aaaaagaatg
     4621 aaagcatatc agatattgca tttgcacata taataaaaag ggtaaaaaat tcatcatgta
     4681 ctcacccaaa agcagcattg gtttttttag gagagaaagg tttttgtgat agcaatgatg
     4741 ttctatctat tatgggacaa caaataccaa gagtatttaa gaacaagatg ttatatgatt
     4801 atgtttttaa aaatgaaaaa agtaaaaatg attttctaaa aatggctgaa tcatggctac
     4861 cacagagtga accaatagta ataaataatg atgatgacgc attgaatgct gctgcttatt
     4921 tttctgtaaa aaaagcgaaa ataaaaacag taaacgatac tgattttaaa gagtataata
     4981 aggtttatat tcttgggcac ggtagtcctg gttctcatca attaggcctt ggttcggaac
     5041 ttattgatgt acaaacaatc atttcaagaa tgaaagactg tggtattcta aatgtgaaag
     5101 atatccgttt tacttcatgc ggctccgctg ataaagtggc tcctaaaaat tttaacaatg
     5161 cccctgctga aagtctttct tgtatcctta actctctgcc tttttttaag gaaaaagaat
     5221 ctttgctaga gcagataaaa aaacaccttg aaaacgatga gtcattgagt gatggtctaa
     5281 aaatatccgg ctatcatgga tatggagttc actatggtca agagcttttt ccctactcac
     5341 attatcgttc aacttcaatt cctgctgatc cggagcatac agtaaaaaga agctctcaga
     5401 aaaagacttt tattattaat aaagaactgg attagtataa aatttttaac ctatagttaa
     5461 ttgtttgcat ttaagatgaa gttattttat ctttagtaga aaagtgcttc cctatgaggg
     5521 aaaatcacaa atcatcataa tacagagaca aaacgatacg aaaaaataga taaaaaacat
     5581 cattctttta ccacattatt ttccgtgaat atgaaaaata atgttattac tttaatataa
     5641 gactattttt tgtttttcca tcactctgtt caaatttttc cgcatgactt gtgttttttg
     5701 taatacagct cgttttttac agctgaccaa aatcatcaat taattatgct aaggaaataa
     5761 attatgaaaa ccaaaaactt tcttcttttt tgtattgcta caaatatgat ttttatcccc
     5821 tcagcaaatg ctctgaaggc agaaggtttt ctcactcaac aaacttcacc agacagtttg
     5881 tcaatacttc cgccgcctcc ggcagagaat tcagtagtat ttcaggctga caaagctcat
     5941 tatgaattcg gccgctcgct ccgggatgct aatcgtgtac gtctcgctag cgaagatgca
     6001 tactacgaga attttggtct tgcattttca gatgcttatg gcatggatat ttcaagggaa
     6061 aataccccaa tcttatatca gttgttaaca caagtactac aggatagcca tgattacgcc
     6121 gtgcgtaacg ccaaagaata ttataaaaga gttcgtccat tcgttattta taaagacgca
     6181 acctgtacac ctgataaaga tgagaaaatg gctatcactg gctcttatcc ctctggtcat
     6241 gcatcctttg gttgggcagt agcactgata cttgcggaga ttaatcctca acgtaaagcg
     6301 gaaatacttc gacgtggata tgagtttgga gaaagtcggg tcatctgcgg tgcgcattgg
     6361 caaagcgatg tagaggctgg gcgtttaatg ggagcatcgg ttgttgcagt acttcataat
     6421 acacctgaat ttaccaaaag ccttagcgaa gccaaaaaag agtttgaaga attaaatact
     6481 cctaccaatg aactgacccc ataaagctgg acagcctgta tcaggctatg gagggcccat
     6541 agacaaatct accctatatg agcataggag gagtctatgg gcacaccacg ttttacccct
     6601 gaatttaagg gattactgga aaggctggga catatccctc cggcagaagc agaaaaagct
     6661 tattatgctg ccatcggaaa cgatgatctg gcaacctgag ttcacagata aaacattctc
     6721 taggaaactc ggggcggttc cgttcaccac atgcaatgtg gtgttgcagg ggaacggtct
     6781 gcaccatccc ctatgtcgat caatataaca gaaatgacaa cttcagattc agggcacaac
     6841 ctaaatatat tttaggtcac ctctcaaatc gtttgcctga taccgctcca ttttttaata
     6901 aaaaaatcaa tcatttttga atctttgctt ttcattgcat tgtcaagcat agtgtcccca
     6961 ctatttgctt tttgaaattt tttgtttaca tcaaccaatc cattattgat aaaatattct
     7021 agcactttat agttggcacc atctttacta agtaagtatt caacatcata cagattacaa
     7081 tatctattgt ttttcatttt ctccatctct gcgacatctt gttttgtaaa attaaattta
     7141 gatagtaaat aaagtgctat atcagctttt ttattgccta aagaataagc aagggcctga
     7201 tgagccattt ttttatcagt cactttattg ttaatctctt cataattaga tgctttaatc
     7261 gcatcctcca aagatatgtc tctgagtttt atttttttga agttatctgt acttagcatt
     7321 cttggtatat gatactcaag ctgaaacaca aagtttataa ttctatccag actaacagga
     7381 tcaatatttt tgttataaca aaactctctg aatccttgat ctttactctt tcttataaaa
     7441 tcaataagat gcaacccaat tccaagcctc atatctttag tgttaaatat tggaacatca
     7501 gttttcccct gattaccatg cacatacctg ctaacttcat ccacaacctc tttaaagtta
     7561 tctaagaaaa atgattggtg ctcatggtaa aaaacaggtg gaata
//
LOCUS       DAJTUP010000156         7512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTUP010000156 DAJTUP010000000
VERSION     DAJTUP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.158"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..990)
                     /locus_tag="OO468_003707"
     CDS             complement(<1..990)
                     /locus_tag="OO468_003707"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="formate acetyltransferase; catalyzes the formation
                     of formate and acetyl-CoA from pyruvate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyruvate/2-ketobutyrate formate
                     lyase"
                     /protein_id="HCR6260661.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSG"
     gene            complement(1045..1902)
                     /gene="focA"
                     /locus_tag="OO468_003708"
     CDS             complement(1045..1902)
                     /gene="focA"
                     /locus_tag="OO468_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR6260662.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(2308..4068)
                     /locus_tag="OO468_003709"
     CDS             complement(2308..4068)
                     /locus_tag="OO468_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR6260663.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            4198..4824
                     /locus_tag="OO468_003710"
                     /pseudo
     CDS             4198..4824
                     /locus_tag="OO468_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            5023..6111
                     /gene="serC"
                     /locus_tag="OO468_003711"
     CDS             5023..6111
                     /gene="serC"
                     /locus_tag="OO468_003711"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR6260664.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            6182..7465
                     /gene="aroA"
                     /locus_tag="OO468_003712"
     CDS             6182..7465
                     /gene="aroA"
                     /locus_tag="OO468_003712"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR6260665.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         1935 a         1787 c         1876 g         1914 t
ORIGIN      
        1 gccagagaac agttcatcgt attccggagt acgcaggaag cgaaccatac gcagtttcat
       61 gaccaggtgg tcaaccattt cctgcgcttc ttgttcggtg atcttgccag ctttcaggtc
      121 acgttcgatg tacacatcca ggaaggtgga ggtacgaccg aaggacattg cagcaccgtt
      181 ctgagactta acagcagcca ggtagccgaa gtaagtccac tggatagctt cctgagcgtt
      241 ggtagccgga ccagagatgt cgtagccgta tttcgcagcc atttctttca tctgacccag
      301 agcgcggtgc tgttcagcga tttcttcgcg cagacggata gtctgttcca ggtttacgcc
      361 gttttccaga tcagcctgta gagaggtgaa ctgagcgtat ttgtctttca tcaggtagtc
      421 gataccgtac agcgcaacgc gacggtagtc accgatgata cggccacggc cataagcatc
      481 tggcagaccg gtcagaacac cggatttacg gcaacgcagg atgtccggag tgtaaacgtc
      541 gaacacgccc tggttgtgag ttttacggta ttcagtgaag atttttttga tcatcgggtc
      601 cagttcgcgg ttgtacgctt tgcaggaacc ttcgatcatt ttgataccac cgaacgggat
      661 aagagcacgt ttcagcggag cttcagtctg cagaccaaca actttttcca acgctttatt
      721 gatgtagcca gcgtcgtgag aggtgatggt ggaagcaaca gcggtgtcaa aatcaactgg
      781 cgcgtgagtg cggttttcca gtttaacacc ttccattact ttgtcccaca gggtggtggt
      841 cgcttcagta gcgccagcca ggaaggactc gtcaccctcg tacggagtgt agtttttctg
      901 aatgaagtca cggacgttta cttcattctg ccagtcacct ttggtaaaac cttcccaggc
      961 tgtggctaac ttttcattaa gctcggacat gtaacaccta ccttcttaag tggatttttt
     1021 atttactgcg tacttcgaca accattaatg gtgatcgttt tcacgcaggt aaatgaccca
     1081 gtatgtcaac ccaaccaaca aaccaccgcc gataatatta ccgatcgtaa ccgggatcag
     1141 gttatcagtg atgaagttca tcacggtcag gtgagaaaaa ttttccggtg cagaaccgac
     1201 agcggtccag aattccggag atgcgaagtc gcggattaca atacccatcg ggatcataaa
     1261 catgtttgcg atactgtgct caaaaccgct ggcaacaaac atcgcgaccg gcagcaccat
     1321 aatgaacgct ttgtccatca ggctgcggcc agaataactc atccatactg ccagacatac
     1381 catcaggttt gccaggatac caagacagac ggcctcaata aaagtatggt gcactttgtg
     1441 gtcggcggtt tgtaggacgt ttagtcccca ttgaccattt gcggtcatat actcgccgga
     1501 aagccacatt aaaagtacaa acagcagtgc gccgaccagg ttgccaaaat agacatttag
     1561 ccagttttta gccaactgac accaggtgat gcgcccactc gccttagcaa caacaatcaa
     1621 cactgtggaa gtaaagagat cggctccgca gacaacacaa agaatcagcc ccagagagaa
     1681 gcaaatgccg ccaaccagtt ttgccatgcc gaagggcatt gtgcctgtgc cagtggttgc
     1741 tgtgatatag aagacgaatg cgattgagat gaaaacaccg gcggtaatcg ccagatagaa
     1801 agtcttaagc ggatgtttcg ttgctttata gacacccgcc tcttcggcca ctttggccat
     1861 tgcagcagga agtaaaagat caaaagggtt gtcagctttc acactaactc tctctttatt
     1921 aagtcggcga cgagatacta acaaagcatt atagatgaga aattgatata gatcatatct
     1981 cgcctggctt ataggcccgt aactcgcatg gtttttatgc aaatacggag taactatttg
     2041 attatccaaa taaaaataaa ttttaaaaat taacaaatga gttgaatttt ttccgcatcc
     2101 tccgctaaaa cagttaatta aaagggagca tcaggcgaat aaagtaacaa tatcgatcgt
     2161 atttattaaa tacaaattac cgatatttaa ccttataatt acaattattt tattaatgca
     2221 aatatatgta aagcggggca ttaaaaaaac gccccgtaat ataactcaga ctaatcatta
     2281 agcctacatt gcgtaggcta tttgatttta ttttgcccag aatgctgctt tggcgcgctg
     2341 cagcttttcg taggccttca acaacgactg atgtgcagca aacgcgtgca gatcgctatc
     2401 taccggttgc aggccgtaaa acgccgcttc gccgctcatt gccgcactgg cggcttctac
     2461 ggcatctgcg ccgtacatgc gaacaaacgc attcagatat tgcagcggct ggcgatcttc
     2521 ttcctgtgcc agtaataaca acgtttgcag gcagcgataa tagttggcgc gttccggact
     2581 aaatactgat gagttaaact ccatcgtcca ttcggtccag accagagcct gttccagatc
     2641 gccaccagcc agcgccagca tggcttttaa ttcaccgata cgcagggtgt accaaccgtt
     2701 atccgacccg gtcgccagac ccaacagctc acgcacgcgg gtaaagtcat caaaaccttc
     2761 ttcatccagt tgctcgatga ggttcaggta atcttctttt tcccactcgc tgcctggtag
     2821 cgaaagaatc gtttcacgta aatggctgcc catactgtta ttcgcgagcc acagatcttc
     2881 agccggataa atatcggaca tgccaggcac gataatacgg caagcataaa cgcccagatg
     2941 ctcgtaatcg gcaatataaa cttctttatc ttctttgttg aagatagcca tcagcgtagc
     3001 aaactcttct tccgtggtgc cggagaaatt ccagtccaca aacggataat cggcatcctg
     3061 cttgaacagg tcccaggaga ttaaaccgct ggaatcgata aagtgcgttt cgaggttggt
     3121 atgttcagcg acttcttcat catcgaaggt tggcggagta aacacatcca aatctttcag
     3181 gccacgacct tgcagcagct cggtcacggt acgttccagt gctacgccaa aatcaggatg
     3241 cgcaccgaaa gaggcaaagc aggtgccgtt tgcaggatta aacagtacca cgcaaatcac
     3301 cggatactgg ccgccaaggg aaccatcata tgcgaagatc gggaaacctt ctgtttccag
     3361 tgtttcgatc gcttcaacca ccgctgggta acgcgccagc acgtctgccg ggatttccgg
     3421 caagctgatg ctttcagcaa taatgcggtt tttcacgtag cgttcgaaaa cttcggacaa
     3481 cccctgaacg cgtgcttcgt tgcgggtatt gccagcggac ataccgttag aaacgtacag
     3541 gttaccaatg atattcatcg gaatataaac ggtctgattg tcggactggc gcgtaaacgg
     3601 cagaccgcaa ataccacgat cttcgttacc ggattgtagg tcaatcagca tactgccggt
     3661 caattcattc tccggatcgt aaaatgcgcg cagacggtca tcgagcagcc cttctggcac
     3721 atcgtcattt tcgctcagtg ggaaccattt ttcgttggga taatgcacaa atggaccgtt
     3781 ggcgatggtt tcgcccagcc agaaatcagc aaaaaagtag ttggttgaga gacgctcgaa
     3841 atattcaccg agtgcagaag ccagcgccgc tttcttggtt gcgcctttac cgttggtaaa
     3901 acacagtgcg cactctttgt cgcgaatatg tacagaccag acgttaggca cgggattcag
     3961 ccaggaggcc tcttcaatct gaaagccgag gtctgaaagt ttttgctgga agcgagcgat
     4021 ggaatcttcc agagcggcat ctttgccggg gataaatgtt tgcgtcatga aaatcacttt
     4081 agtcgtacgg aaagcgcgca ataatacggg ttttatctca aaggcgctat caccgccgcc
     4141 atgccgatga actgttgact atgcttttag cggataaacc acgttaagag cataaaaatg
     4201 aaggcgttcg atctccaccg tatggcattt gataaagtgc cttttgattt ccttggcgaa
     4261 gttgcactac gtagtcttta tacctttgta ctggtttttt gttcctcaaa atgaccggaa
     4321 gacgcggtgt gcgacagatg tcgctgtttg aagtgttaat cattctcacg ctgggttcag
     4381 cggcgggaga tgtggcgttt tatgatgatg tgccgatggt gccggtactt atcgtcttta
     4441 tcactctggc gttgttatac cgcctggtaa tgtgatggcg agctggcctg gtcgaaactc
     4501 aataactcca acatgacgga atttgagttc tttatggagc tacgattgcg tggcgtggag
     4561 cagctggggc aggtacgtct ggcgattctc gaaaccaacg ggcaaatcag tgtctatttc
     4621 tttgaagatg acaaggtgaa accgggttta cttattttac ccagtgattg tacgcagcgt
     4681 tacaaagtgg tgccggagtc ggcggactat gcctgcattc gttgtagtga aatcattcat
     4741 atgaacgcgg gggaaaaaca attatgtccg cgctgtgcaa atccagaatg gacgaaggca
     4801 agtcgggcaa aacgggtgac ctgacagtaa aaacatcggc tttttgctaa taatccgaga
     4861 gattcttttg tgtgatgcaa gccacatttt tgccctcaac ggttttactc attgcgatgt
     4921 gtgtcactga atgataaaac cgatagccac aggaataatg tattacctgt ggtcgcaatc
     4981 gattgaccgc gggttaatag caacgcaacg tggtgagggg aaatggctca aatcttcaat
     5041 tttagttctg gtccggcaat gctaccggca gaggtgctta aacaggctca acaggaactg
     5101 cgcgactgga acggtcttgg tacgtcggtg atggaagtga gtcaccgtgg caaagagttc
     5161 attcaggttg cagaggaagc cgagaaggat tttcgcgatc ttcttaatgt cccctccaac
     5221 tacaaggtat tattctgcca tggcggtggt cgcggtcagt ttgctgcggt accgctgaat
     5281 attctcggtg ataaaaccac cgcagattat gttgatgccg gttactgggc ggcaagtgcc
     5341 attaaagaag cgaaaaaata ctgcacgcct aatgtctttg acgccaaagt gactgttgat
     5401 ggtctgcgcg cggttaagcc aatgcgtgaa tggcaactct ctgataatgc ggcttatatg
     5461 cattactgcc cgaatgaaac catcgacggt atcgccatcg acgagacgcc agacttcggc
     5521 aaagatgtgg tggtcgccgc cgacttctct tcaaccattc tttcccgtcc gattgacgtc
     5581 agccgttatg gtgtaattta cgctggcgcg cagaaaaata tcggcccggc tggcctgaca
     5641 atcgtcatcg ttcgtgaaga tttgctgggc aaagcgaata tcgcgtgtcc gtcgattctg
     5701 gattattcca tcctcaacga taacggctcc atgtttaaca cgccgccgac atttgcctgg
     5761 tatctatctg gtctggtctt taaatggctg aaagcgaacg gcggtgtagc tgaaatggat
     5821 aaaatcaatc agcaaaaagc agaactgcta tatggggtga ttgataacag cgatttctac
     5881 cgcaatgacg tggcgaaagc taaccgttcg cggatgaacg tgccgttcca gttggcggac
     5941 agtgcgcttg acaaattgtt ccttgaagag tcttttgctg caggccttca tgcactgaaa
     6001 ggacaccgtg tggtcggcgg aatgcgcgcg tctatttata atgccatgcc gctggaaggc
     6061 gttaaagcgc tgacagactt catggttgag ttcgaacgcc gtcacggtta atgccgaaat
     6121 tttgcttaat ccccacagcc agcctgtggg gtttttattt ctgttgtaga gagttgagtt
     6181 catggaatcc ctgacgttac aacccatcgc tcgtgtcgat ggcactatta atctgcccgg
     6241 ttccaagagc gtttctaacc gcgctttatt gcttgcggca ttagcacacg gcaaaacagt
     6301 attaaccaat ctgctggata gcgacgacgt gcgccatatg ctgaatgcat taacagcgtt
     6361 agggttaagc tatacgcttt cagccgatcg tacgcgttgc gaaattatcg gtaacggcgg
     6421 tccattacac gcagaaggtg ccctggagtt gttcctcggt aacgccggaa cggcaatgcg
     6481 tccgctggcg gcagctcttt gtctggatag caatgatatt gtgctgaccg gtgagccgcg
     6541 tatgaaagag cgcccgattg gtcatctggt ggatgcgctg cgcctgggcg gggcgaagat
     6601 cacttacctg gaacaagaaa attatccgcc gttgcgttta cagggcggct ttactggcgg
     6661 caacgttgac gttgatggct ccgtttccag ccaattcctc accgcactgt taatgactgc
     6721 gcctcttgcg ccggaagata cggtgattcg tattaaaggc gatctggttt ctaaacctta
     6781 tatcgacatc acactcaatc tgatgaagac gtttggtgtt gaaattgaaa atcagcacta
     6841 tcaacaattt gtcgtaaaag gcgggcagtc ttatcagtct ccgggtactt atttggtcga
     6901 aggcgatgca tcttcggctt cttacttcct ggcagcagca gcaatcaaag gcggcactgt
     6961 aaaagtgacc ggtattggac gtaacagtat gcagggtgat attcgctttg ctgatgtgct
     7021 ggaaaaaatg ggcgcgacca tttgctgggg cgatgattat atttcctgca cgcgtggtga
     7081 actgaacgct attgatatgg atatgaacca tattcctgat gcggcgatga ccattgccac
     7141 ggcggcgtta tttgcaaaag gcaccaccac gctgcgcaat atctataact ggcgtgttaa
     7201 agagaccgat cgcctgtttg cgatggcaac agaactgcgt aaagtcggcg cggaagtgga
     7261 agaggggcac gattacattc gtatcactcc tccggaaaaa ctgaactttg ccgagatcgc
     7321 gacatacaat gatcaccgga tggcgatgtg tttctcgctg gtggcgttgt cagatacacc
     7381 agtgacgatt cttgatccca aatgcacggc caaaacattt ccggattatt tcgagcagct
     7441 ggcgcggatt agccaggcag cctgaatgaa caacgggcaa taaatagcca aatctttctt
     7501 tatcaaaacg tc
//
LOCUS       DAJTUP010000157         7475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTUP010000157 DAJTUP010000000
VERSION     DAJTUP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.159"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(90..1480)
                     /gene="tcyP"
                     /locus_tag="OO468_003713"
                     /pseudo
     CDS             complement(90..1480)
                     /gene="tcyP"
                     /locus_tag="OO468_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(1613..2203)
                     /locus_tag="OO468_003714"
     CDS             complement(1613..2203)
                     /locus_tag="OO468_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR6260666.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2366..3034)
                     /gene="hxpB"
                     /locus_tag="OO468_003715"
     CDS             complement(2366..3034)
                     /gene="hxpB"
                     /locus_tag="OO468_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR6260667.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3181..3717
                     /locus_tag="OO468_003716"
     CDS             3181..3717
                     /locus_tag="OO468_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR6260668.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(3758..4618)
                     /locus_tag="OO468_003717"
     CDS             complement(3758..4618)
                     /locus_tag="OO468_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR6260669.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4724..5014)
                     /gene="ghoS"
                     /locus_tag="OO468_003718"
     CDS             complement(4724..5014)
                     /gene="ghoS"
                     /locus_tag="OO468_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR6260670.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5115..6044)
                     /gene="pfkB"
                     /locus_tag="OO468_003719"
     CDS             complement(5115..6044)
                     /gene="pfkB"
                     /locus_tag="OO468_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR6260671.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6331..7089
                     /locus_tag="OO468_003720"
     CDS             6331..7089
                     /locus_tag="OO468_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR6260672.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7142..7249)
                     /locus_tag="OO468_003721"
     CDS             complement(7142..7249)
                     /locus_tag="OO468_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260673.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         1928 a         1844 c         1824 g         1879 t
ORIGIN      
        1 aatatgagtc agtatgcatc gccgcgatgg aacatttaaa aagtaaagca acggccccta
       61 tacaaaacgg accgttgcca gcataagaat taacggtgtg ccagttcggc gtcgtcttcg
      121 ctatccagaa tggctttatc ggtttgcttc agccactggc tggtcagcgt gccagctgtc
      181 atcgagccac taacgtttag cgccgtacgg cccatgtcga taagcggttc aacggagatt
      241 aacagcgcca ccagggttac tggcaggccc atcgcaggca gcacaatcag tgcggcgaaa
      301 gttgcaccac caccgacacc ggcaacgcct gcggaactaa cggtaacaat accgaccagc
      361 gtcgcaatcc acatcgggtc cagcgggtta atgccaaccg taggcgcaac catcaccgca
      421 gcattgccgg atacaaaccg gcgcagccgt tctgaccaat ggttgcaccg aaagaggcgg
      481 cgaaactggc gatggattca ggaacgccca gacgacgcgt ttgtgcttcc acattcagtg
      541 ggatagacgc agcactggaa cggctggtaa aggcaaacgt cagcacaggc cataccttac
      601 ggaagtactt cagcggactc acgccattaa tgcccagcag aatgccatgc acagcaaaca
      661 taatcagcag accgaggtag gacgcgacaa cgaaacttcc cagtttgatg atgtcttgca
      721 agttagaacc tgcaaccact ttggtcatta gagccagaac gccgtaaggg gtcaactgca
      781 tgacaaggcg aaccagtttc atcacccagc tttgtagggt atcgatagcg gttaagacgc
      841 gttccccttt cggcgcatca tccttcagca gtttcaatgc agctacgccg aggaatgcgg
      901 caaaaattac cacgctgata attgacgtcg gattggctcc ggtaagatcg gcaaacgggt
      961 ttttcgggat aaaggacaag accagctgcg gaacgctctg atcagagact ttaccaacat
     1021 agttactttc aatggcgttc agacgtgcag tttctgcacc accctgaacc agaccttcag
     1081 ccgtcaaacc aaacaggttg gtgaccagca caccgaccag cgccgcaatc agcgtggtaa
     1141 acaaaagcgt accgatggtc agaaaactga ttttgcctaa ctgagatgcg ttatgcagac
     1201 gggcaaccgc gctcagaata gaggcgaaga ctaacggcat aacgatcatt tgcagcagtt
     1261 gaacatagcc gttaccaacg atgttaaacc actgtacaga atctttaagt acctggctgt
     1321 cagaaccata aatggtatgc agggcaaggc caaaaaccac acccatcacc agacccacca
     1381 gcactttttt cgccagactc cactgtttat ggcgggtctg agccagcgca aacagcagta
     1441 caacgaacac cacgatgttc gcaattaatg gaaagttcat ccccgttctc ctgatcttat
     1501 tatttctatc actgcgtagc agtcatagtg ttggcgcaag attaacagaa ctgtgagctg
     1561 gctcttatat tcaaatggaa ttgcttattc cagaaaggtg atttttgcct ttttagtatt
     1621 caacctgatg cttaataagc cgatgaaact ggatctgcaa ggtattgatc atttgcgatg
     1681 accaacgaac agcgctgttc tcgggtaatg aatggggcat ccataccgac cagacaaata
     1741 atgccatgca gataaaacgt tccagttggt tgcccttttg gggaaccagg ataggcaagc
     1801 ggaaacgcca gcggcatggc cagagcaggg gaacaccggc gggtgtcagc atatcggcaa
     1861 gtatgtggct caaataaccc agcaccattc cttgtagcgc atcagccgga atgaaccagc
     1921 cttccggaac cttaaggtaa aaggttgcca gcagcgcaaa taccgccagc agactgtggg
     1981 taaaaccacg atgcccaaaa gcgcgggcga tcggttttga tatccatttt aatcgctgcc
     2041 caagaaacga ctttgggtga tcgatgtccg gtaacaaaca cgtcaggatt gcggaaggga
     2101 caatatgcca ccagtcaccc tgtgccagca cgggcgtcag ctcggcattt ttggcaaata
     2161 ccgcacaagc aatagaaaag agaaggtgac cttccgccgt catgataaaa ctcactaaac
     2221 tgtcgatacg tacagtatag gatttttata cagtgcacgg aagtggtgac ggtgtaactc
     2281 tttctcttaa attgacgatt aatccgtcag ttgaatgcaa attcagataa agaaccaggg
     2341 gcagcgtgat cactgcccct ggtcattaac cgagaaggtc ttttgcggtg agttctgtca
     2401 gcgatgagag tttgacgttt gctaatacaa aacgtggatc attttgcgct tctggcgcag
     2461 gaacgacgat ggaacgcatg cgggctgctt tagaagcgat catgccattt accgagtctt
     2521 ccagcgccac gcaggtcagt gggtcaacgc ccagttttgc tgcgcagtcg agatatactt
     2581 gcggatgcgg cttgctgtaa ggcagttttt cagccgaggc gagggcatcg aaactgtcgc
     2641 gtaagtcaaa catggtcaac actttttcca gcatatgtag tggtgacgcg gaggccagtc
     2701 ccaccaataa accttgttct ttgcataacg caacagcttc gcgcacgcct ggtaataatg
     2761 gacgtgtctc ttcaaccagt gaaatggcac gggcaataac ccgttctact acttcctgac
     2821 ggcttggccc attccacggt tgccgggcgt accaaagatc gaccaccata tcgatgcgta
     2881 aacctaaggt gtccggcagc tcgttacgac gggagatatc tacccccagg cttgccatca
     2941 catccagttc ggctcgatcc cataaaggtt ctgagtcgat aagtaatcca tccatatcaa
     3001 aaattgcagc aagaatctga cgcggggttg acattacaac ctctccttta gagcattatg
     3061 tgtaaggtat taagccgtgc ttatgattaa gcgcctatca tatcgccttt aaagaatggg
     3121 cgaaaacaga tatctacagg taaactaagt gaaacgacgc gtcttaatca aggagaactc
     3181 atgacgtatc aacaagctgg acgcattgct gttttgaaaa ggattttggg ttgggtgatt
     3241 tttatacctg cattgatctc tacattgatt tctttgctga aatttatgaa tacccggcag
     3301 gaaaaccagg aaggcattaa tgcggtcatg ctcgactttg ctcatgtcat gatcgatatg
     3361 atgcaggcga atacgccttt tttgaatctg ttctggtata actccccgac ccctaatttt
     3421 aatggtggtg tgaacgttat gttctgggtg attttcatcc tgatttttgt cggactggcg
     3481 ttgcaggatt ccggtgcccg gatgagtcgc caggcgcgct tcttgcggga aggtgttgaa
     3541 gatcaactca ttctggaaaa agccaaaggg gaagaggggc taacgcgtga acaaattgag
     3601 tcccgtattg ttgttccgca ccatactatt ttcctgcagt ttttctcact atatatcctg
     3661 ccggttattt gtattgctgc aggttatgtg ttcttttctc tgcttgggtt tatttaatca
     3721 aaaggccgca cggagcggcc attatcctca acccatatca tgctgctaat aatctatcca
     3781 atgactgctg agcaataacc aaatgctgac cgccaaataa tcttgcacga tttaacagcg
     3841 tgtagagttg gtaaaccggt tgacgttcaa ggaaatcggc aggtagcggg gatactgact
     3901 gatagccgtc atagatttgt ggcggctgtt cagtatgcag cggtaacatc gctaggtcgc
     3961 actctcggtc accccagtcg caggcagggt cgaaaatgta cgggccatcc ggacccagcg
     4021 cacagttgcc ggaccataaa tcgccgtgca acagagaagg ctgcggttga tgcgaggcca
     4081 gacgctgctg aatatgctcg acgatggcgt cgatattgcc gaaagcgatc cctttctctg
     4141 ctgccagttc caactgccag ccaatccgtt gttcagcaaa aaacgttgac cagcgacgtt
     4201 gccaggtgtt gggctgtgga gttgtggaga gcgagttatc aaaatcgagg ccaaattgtg
     4261 gttggtcact ccattgatgt aaacgcgcaa tttgctgacc aagaataaat gcgctatgcg
     4321 catccagcgg acgaggtggg agataatcca tcaccagaaa actgtagtca cggtcagcgc
     4381 ctactgccca aaccttaggc acggtgacgg ttttactacg cgacagtaac tccagttggt
     4441 cggcttcggc ggtaaaaccg ggaagcagtt ccctttcatc acatttgacg aaaaagtcat
     4501 gtcctgcata gcgcaaatgc catgcggcat ggacttctcc gccaggcagt tcattacgca
     4561 gttcgatttc gccttcacct aactgctcgc tcaaaagacg actgattgcc tgccacatga
     4621 tgtctctccc atgttgtctg ccagatcata aggttagcgc aagaatgcgg tgtaaaaaca
     4681 cgagctaacg cacaccacag actgattatt gcccaaaaac catttatttt ctgttgctat
     4741 tccattcctc ccaggtcgtg atggtgattt caggatcttt gccggttgca ctttgggtaa
     4801 gccccgaaac cagttctcta atttcttctt cactttgggt actgataagt ccaaaagtgt
     4861 tagtccccag ctcatggata tttccctcat catccgtcat ggtgagcaaa aaaccgtcgc
     4921 gagtcaggta attattcaac tcgttaattt cagtcaatgt atcctcatgc aacatgacgg
     4981 ttatgacata atggacaaga tcgccactgg ccatagttca cccctttgtt atcacatgac
     5041 ttttgagcat agtcggagaa acgcgttgcc gacaggttgg tggtgattcc cccaatgctg
     5101 ggggaatgtt tttgttagcg ggaaaggtaa gcgtaaattt tttgcgtatc gtcatgggag
     5161 cacagacgtg ttccctgatt gagtgtggct gcactccccg cagctacgcc aaaacggacc
     5221 atctcttcaa gagaggcatt ttctgccagt ttcagtgtca tcgcgccgac catgctgtca
     5281 ccagcgccaa cggtactctg gcttttcacc ggtggtggca ccacctgaat gcagttttca
     5341 ctatcgacac ccagcgctcc ttgtggacca agggaaacga caacccgttt ggccttgccg
     5401 ctattaacga tttcctgcgc ggctttgcgg acatcgtctg gctgggtgag ttcgcgattc
     5461 accagcgcac tgagttcttt ttggttaggc ttaaccaact cgatgttacc aatcgccagt
     5521 gctgcactta acgcttcgcc agaactgtcg atgatgcagc ggatcccttg gttttgcgca
     5581 gcggaaatca gttgggttaa tttttccagc ttcacacctg gcggcaggct tccgcttatg
     5641 accaggatgg ccccggattc aatttccaga acttgctctt caagctggcg aaactcatct
     5701 tcatttaatg ccgcgcctgg cataacaaaa cgatactgtt caccgcttgc ttccacatgt
     5761 acgtgtaaat tctgccgggt ccagtctttg gcttctacag tagcgacggg gacattttca
     5821 tccgccaaca gtgaaaccag gtgttcgccg gtcgcgccac ccgccgggaa gatcgctgtg
     5881 gcactgcctc caagatgggc aatggcgcgg gcgacgttga tgccgccgcc cccgggttcg
     5941 aacaccggtg cggtacagcg cagttttcct tcgggataaa tttgcggggt aattgttgcg
     6001 ctatcgagag agggcgcaag tgtcaacgta tagatacgta ccatcatttc ctcctatagg
     6061 ctgatttcag tctggcaccg aatcagcgga aagtgaagaa attaacaata tgatttatta
     6121 gagctttatt taaaattttg cagataaata tatataaata aaaatctgtt ttcgccacat
     6181 gcttattcaa aaaatgaaat aagaatacat tgctaagtta ttcgcgcctt tttataattc
     6241 gttacctttc cagggacgct aatctttgat ccttaagaaa gtttccggga ccgcgatggt
     6301 ctcttttttt gttgtacgga ctcttgataa atgaagcttt tgaagacagt tcctgccatc
     6361 gttatgttgg cggggggcat gtttgcgtca ctgaatgcag ctgccgatga ttccgttttt
     6421 actgtcatgg acgatcctgc cagcgctaaa aaacctttcg aaggtaatct gaacgcagga
     6481 tatcttgcac aatcaggcaa tacgaaaagc tcctcactta cggctgacac caccatgacc
     6541 tggtatggac aaaccactgc ctggtcgctg tgggggaacg ccagtaatac ttcttctaat
     6601 gatgagcgtt cttcagaaaa atatgcggcg ggcgggcgta gccgctttaa tttaactgat
     6661 tatgactatt tatttggtca ggcaagctgg ctgacagacc gttataacgg ctatcgagag
     6721 cgcgatgtgt tgaccgcggg ttatggtcgt cagtttttga atggcccggt acacagtttc
     6781 cgctttgaat tcggtccagg tgtgcgttac gacaaatata ccgacaatgc cagtgaaacc
     6841 cagccgctgg gctatgcctc cggggcttat gcatggcagt tgactgacaa cgctaaattt
     6901 acgcagggtg tttcggtctt tggcgcggaa gacacaacgc tgaactccga aagcgcatta
     6961 aatgttgcta ttaatgaaca ttttggactg aaggtggctt acaacgtcac atggaactct
     7021 gaacctccag agtctgcacc ggaacatacc gaccgtcgga caaccttatc gctgggctac
     7081 agcatgtaaa attatctgcc ggaccgtgtt atgcggtctg gctgtcttcg tttgtcactg
     7141 ctcagcccca aatcattgct gcccaacgta gcaacaacat agcgccacag attgccagta
     7201 gcaccaccac catcttccag tgttttttat caaagcgttt agtcggcata ccagtgtcct
     7261 gcatatctct tgtgcgtgat aacggagtgt aacaaaaggg acaggcatca agggattttt
     7321 tgttattaca gccgtcgggt ggcgttgtgc acatggtcac tgtttctgcg ttttatgaaa
     7381 aaaataaaat tatgtatcga tatatatcgt aaatagctgg ttataaatac gtctttattt
     7441 catcagcact aaaactcagg ctttggctga tgaca
//
LOCUS       DAJTUP010000158         7437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTUP010000158 DAJTUP010000000
VERSION     DAJTUP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.160"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..987
                     /gene="yjeM"
                     /locus_tag="OO468_003722"
                     /pseudo
     CDS             1..987
                     /gene="yjeM"
                     /locus_tag="OO468_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1138..4449)
                     /gene="mscM"
                     /locus_tag="OO468_003723"
     CDS             complement(1138..4449)
                     /gene="mscM"
                     /locus_tag="OO468_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR6260674.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4471..5439)
                     /gene="psd"
                     /locus_tag="OO468_003724"
     CDS             complement(4471..5439)
                     /gene="psd"
                     /locus_tag="OO468_003724"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR6260675.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5536..6588)
                     /gene="rsgA"
                     /locus_tag="OO468_003725"
     CDS             complement(5536..6588)
                     /gene="rsgA"
                     /locus_tag="OO468_003725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR6260676.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            6683..7228
                     /gene="orn"
                     /locus_tag="OO468_003726"
     CDS             6683..7228
                     /gene="orn"
                     /locus_tag="OO468_003726"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR6260677.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1685 a         1952 c         2007 g         1793 t
ORIGIN      
        1 tctgaattta gtattgctgt tagtaagcat tactattttg ttattaaatg gtgggcattt
       61 cgcgcaggat attaatttcc ttgcatcacc gaacccgggt tatcagtccg gtctggcaat
      121 gctatcgttt gtggtatttg ctatttttgc ctatggcgga attgcagcgg ttggtggtct
      181 ggtcgataaa acggaaaatc cagaaaagaa ctttgccaaa ggtattgttt ttgccgctat
      241 tgttatttca atcggttatt cgctggcaat atttttatgg ggcgtcagca caaactggca
      301 gcaggtatta agtaatggtt ccgttaacct tggcaatatt acctatgtgc tgatgaagag
      361 cctcggggtg atgctgggta acgcactgca tttgtcacct gaagcgtcat tgtcgctggg
      421 cgtatggttt gcgcgtatta ccggactttc gatgttcctc gcttataccg gtgcgtttgc
      481 tattcaccgc tgaaagccat catccagggg acgccgaaag cgttgtggcc ggaaccgatg
      541 acgcgcctga atgcgatggg gatgccgtct atcgccatgt ggatgcagtg cgggttggtt
      601 actgtcttca ttctgctggt ttcgtttggt ggcggtaccg catcggcgtt ctttaacaag
      661 ctgacgctga tggcgaacgt gtctatgacg cttccttacc tgttcctcgc gctggctttc
      721 ccgtttttta aagcacgtca ggatctcgac agaccatttg tgattttcaa aacgcgtatg
      781 tcggcaatga ttgcgacggt ggttgtcgtt ctggtggtga catttgcgaa cgtcttcacc
      841 attattcagc ctgtggttga agccggagac tgggacagca cattgtggat gattggcggc
      901 cctgtcttct tctcgctgtt agcgatggcg atttaccaga actattgcag tcgcatggcg
      961 aataaacctg agttagctct cgactgatca tatgaaatcc tgcaactcat cgtctctccg
     1021 gcgatgagtt gtcaggaagt aatgcgtatg gatgatactc tccagggccg gtgataactg
     1081 aagatgatgt taagctgcta aatgagccac gttgacatca tttggcagct tcatgcatta
     1141 caaacttccc gcctgacgac ctttgcccgc agacgtcagc gttctccccg tttgtttacc
     1201 gttgaggctt tccagacgca tctggaaggg cgggaatggc atatcgatac cgtgggcgtg
     1261 gaagcctgcc agaatcagct ggtggatctc atggcgtagc ggcatacggt gacccatctc
     1321 agcggcgtaa atacgcagct cgaaaatctg aatcccctgt tgcagatcca ccaggaagac
     1381 ttccggtgcc gggttgtcga tcaccagcga gcagcgacgc gctgcggtga gcaggatttc
     1441 cgtcacttct tcactgttgg catcggcagg ggccggaatc gtcaacacta cgcgcgtgac
     1501 cgagtcagag agcgaccagt tgataaactg ctcggtaata aacgccttgt tcggcacgat
     1561 tatctcttta cggtcccagt cgctgatggt ggtggcgcgg gtgttaattt ttgtcacgct
     1621 accagtaaga tcgcggatcg ttaccgtatc gccaatgcga atcggttttt cgaacaggat
     1681 gatcaggcca gagataaagt tggcgaaaat ttcctgcaaa ccaaaaccga gaccaacacc
     1741 gagcgcggca accagccact gcaatttcga ccactcaata ccaatcattg agaagccgac
     1801 cagcccgcca atcagcatca gcagatattt tgtgatggtg gtgatggcgt aacccgtacc
     1861 cggcgttaaa tccaggtgct gcaaaatcgc cagttccagc agcgcgggca ggttgcgcac
     1921 cagctgcgtg gtgatgataa ataccagaat ggcaatcagc accgcaccga gggtaattgg
     1981 ctccagactt tctacgccct gtaccgtgga ggtgacatcc cacagcgaaa tattttcgag
     2041 gaagccgaaa gcggaatgga tttctgacca cagcacaatg accgaaagca gggcgatcaa
     2101 catcaaaatt gagcgcacca gccgcaagga ttgcgcactg atggcatcga gatcgacttc
     2161 gctttcatcg acttcaattg ctccttccgg gctactgtga tgatgcgctt cctcttcgcc
     2221 acgcgcacgt tgcgctaaca tctctgcccg gcgatgcttc gcccggtcaa acgccagcct
     2281 gcgacgctgg atcagcatcc agcggcggat aacgtgataa accaccagta gcaggaacca
     2341 gatggcaacc gaggtttcaa gcctcgctaa cagtgcctgc gccgttgcca gataacccac
     2401 cgccgacgcc agaatggcaa ccagtggtgc gccaatcatc atgttccaca gcatatggtt
     2461 ggtaatgttg tcgccgctgc cttctttgtt gagatacaac ggaataccgg cttttttcag
     2521 gcttagggtg accaccgcca gcgcaccgca aatgaggata aagcaaagcc gtcccagcga
     2581 accggaaaat tcgcggtcgt cgaggttatc gaacatcatc agcgccataa tcagcccgat
     2641 gctcatcagg tagtagcgca tcccacggga aacacgttcg cgcggccagc caaaatgagc
     2701 gataaacaag ccgttcgggc gggcaaaggt ggcgcaaatc atcactaccc acagcagcgg
     2761 cacggtggcc gttacaccat cgccaatcgc gaccgccagc ggataaggcc acgcctcgcg
     2821 caagccgtaa cccagcgtca tccacagcac cggtaacggt gacgcgacga gaatcgacca
     2881 gaaaagagtg cgcaacgtca gccagaagtg atcctgagtc actttgccga ctttcgccgc
     2941 cgaacgttca agaaaacggg tgaaatagcg gcgcgagtaa atactgcaac cgaccagaat
     3001 caacgcgcca aacagcggca aaattgtctc tttgctggtc agcatcatca cactggcttt
     3061 gcccaactga ctgaaggtgt ccagcgaaat gagacgacgc agatcctggg cgatttccag
     3121 cggccaggcg atggtcatcg ggcgcacgtc agaggcccag aacagatagc ggtgcgttgc
     3181 ttcgttcacc tctttcagcg catcctccag ttgcctgttg gagactttca gcttggtcag
     3241 ttccaatagt agcgtgtcgc caccctgcaa caatgagttc agcaactcac gctgagtgcg
     3301 cagttgtgct tccagaatac ggttttgctc ggcggtcagc ggctgaccgt cggcctgatg
     3361 gatttgccgt agcagcggct gtttattaag taaatcctca taacgtaacc gttgcacacg
     3421 caactgcgcc atttcggtat caagctgttg tggttttggc atttctggta gccgtgccac
     3481 ctgcgcccgc agcgcttcgc cgagcaggtt ggacgatccc agccattgcg actgttcacg
     3541 cagcgtattc aacgcctgcc ggacctgtaa cgtctggctg gcagcctgac gctgttgcga
     3601 ggcaacgagc tccatccgct gcgcctgttg attcagcgct gccgatagtt cgcggttaat
     3661 tttgaattgc gcgacgatat ctttcggcaa atcggcactg ttttctgcca gtaattcggt
     3721 actttccagc gcccgctccg cctcaagctg acgttggcta ttcaactgat tacgtaaggc
     3781 ctgcaaatac gcatccagtt gctggctctc tttttccgcc agctctgagc gtaagcgcgc
     3841 taattcctgg cggttattgg cagacagctg cgccagctcc agttcatcaa cgagcgcctt
     3901 aagacgtgca gagtcagact gcaacgcgaa attttgtgcc tgattgagcg gagtattgcc
     3961 ggtaagcgtt cccaggcggc gctcgatctc atttaactga cggcgggcgt cggtttgctg
     4021 ttgcggcagt tgattcagcg aatcggcaat ctcgcgggcg cgctcctgct cttgctgggc
     4081 ctgacggctt ttatccagca actggctgct gacctggaga atttcctgat tcagcgcgtc
     4141 ggtagacatt cccggcgaca cgctgcgcgg ctcgtcacga atgttgttta attgtgcgcg
     4201 cagagtagcg gagagtttcg gataattatc gataacttgc tgatattgtt tgatgcgctc
     4261 aagggaacct tttcgttcct caagcgcatt taaggcagac tggagcgcct ctacgacttc
     4321 cggctgtgcg ggtttcgccg cttttgcctg ctccagttcc tgagtgattt gtttgctatc
     4381 gggggccgtc gcggcgtacg ccccccaact gaggcaccag gccatcagaa aagtgataat
     4441 caggcgcacg tcagcgtttc ctttgatgga ttagacctgg tcttttttat cgtcaaccaa
     4501 tgggctggcg tcgtgttctg cttcgatctc ttcagcagga agtggggcgg gttcagcgtc
     4561 tggcgtaaca aaggtttcgg tagatactgc cagcggctgg ccaattttcg tgacagacag
     4621 gctttccagt tgctcaacca gattcacttt acccggtgca aacaggttga taacggtgga
     4681 accgagttta aagcgaccca tttcctggcc tttcagcagt gccacagaac cgtcgttttc
     4741 cccggcaggc caggtccagc gcttgatgat accttcgcgc ggcggcgtaa tggtgcccgc
     4801 ccagaccgtc tcaatgctgc caacaatcgt cgctccgacc agaatctgcg ccattgggcc
     4861 aaattcggta tcgaaaaggc aaatcacgcg ttcgttacgg gcaaacagat ttggcacgtt
     4921 ctgagcggtg agatggttaa cggaaaagag atcgcccggc acgtagatca tctcacgcag
     4981 aataccgttg cacggcatgt gtacgcgatg gtagtcacgc ggggagaggt aagtggtcac
     5041 aaacgtaccg ttgcggaaca ggtccgccat cagatagttg cctgccagca gggcttcgag
     5101 gctgtagttg tggcctttgg cttgcaggat tttatcttct tcgattttac ccaactggct
     5161 gataacgcca tcggcaggca tgaccagtac attcggatcg gtatcgattg ggcgtacttc
     5221 gtcacgcagc ggacggacaa agaattcgtt aaaggtgcgg tagctggcgg tgtccggctt
     5281 ttgcgcctct ttcatgtcga ccttgtagta tttaacgaac agatcgataa ccagttttgt
     5341 cagccatcct gcccgcttgc ttgcgcccca acccgccagg cgagtaagcc atagtttcgg
     5401 cagaatgtac tgtagcgaaa gtttaaatga atttaacaag gtagcctcca ggccattgtt
     5461 ttgtcgttcc tgatccggcc aacatgccgg atcctgaaaa aaaggggacg attctaacga
     5521 cggttagctt aattgtcagt catccgtatc agaaaagttt ttacgcgttt ttacctgcgc
     5581 catgctttcc agaatacggt gatagttttc gaaacgggtt tccgcgattt tcccttcctc
     5641 aaccgcttcc cggatagcgc agcccggatc ggtatcgtgt ttgcaatcgc gatatttaca
     5701 cagacctaaa tagtcatgga attcgacaaa gccctgagtg atttgttccg gctccaggtg
     5761 ccagaggccg aactcacgca caccagggga atcaatcaca tcaccgccgt gcgggaagtg
     5821 atacagccga gcggcggtcg tggtgtgctg accgagcccc gagttgtcag agacatcgtt
     5881 ggtcaggatc tctttttgca gccccagcaa cgcgttcagc aggctggatt taccgacgcc
     5941 agactgcccg gcaaaaatgc tgatgcgccc ggtcaacgcc tcttccagcg gtttcagccc
     6001 atcctgagta tggctggaaa ccatcaatac gcgataaccg atgttgcggt agatatccat
     6061 ctgctcgttg acgaacgcca tgccttcgtc gtccagcagg tctatcttgt tgagcacgat
     6121 aatcggctca atctgcaagg tttcgcaggc caccaggtaa cggtcgataa tattgagcga
     6181 cagctccggc aaaatggcgg agacaatgac aatctggtcg atgttggcgg ctatgggttt
     6241 cacgccgtcg tagaaatccg gacgcgttaa caccgaggtg cgctcatgca ccgcttccac
     6301 gatccctttg acatttacgc cttccgccgc cggtttaccc ggacgccaga ctacgcggtc
     6361 gccggttacc agcgaacgga tggtacggcg aatattgcag cggtgaacgt cgccatcggc
     6421 ggattccaca tcagcgtgca taccaaagcg gctgatgacg ataccttcat caggctcgcc
     6481 aaacagattg tcgtcgtagt cgggcttctc cttagacgtt ttaagacgac gctggtgatt
     6541 ggcgttcacg cggcgctgct ggcctttgga gagtttattt ttactcaatc gtactgactc
     6601 ctggtcgccc ctgatgggca aaacatctat gatacacgca attgtggatc aatatagtca
     6661 ctgtgaatgg gtggaaaata gcatgagtgc caatgaaaac aacctgattt ggatcgatct
     6721 tgagatgacc ggtctggatc ccgagcgcga tcgcattatt gagattgcca cgctggtgac
     6781 cgatgccaat ctgaatattc tggcagaagg gccgaccatt gcagtacatc agtccgatga
     6841 acagctggcg ctgatggatg actggaacgt gcgcacccat accgccagcg ggctggtaga
     6901 gcgcgtgaaa gcgagcacga tgggcgatcg ggaagctgaa ctggcaacgc tcgaattctt
     6961 aaaacagtgg gtgcctgcgg gaaaatcgcc gatttgcggt aacagcatcg gacaggaccg
     7021 tcgtttcctg tttaaataca tgccggagct ggaagtctac ttccactacc gttatctcga
     7081 tgtcagcacc ctgaaagagc tggcgcgccg ctggaagccg gaaattctgg atggttttac
     7141 caagcagggg acgcatcagg cgatggatga tatccgtgaa tcggtggcgg agctggctta
     7201 ctaccgcgag cattttatca agctgtaaaa tttttcctgg tcacgtaagc gccggaatcc
     7261 acttgcggcg gcgtgatttt gacgctaaat cgaacgtctt gctgataatt tcagcacttg
     7321 agataaaaac gcaaaaaaaa cttttttggg gggttgcaga gggaaagatt tctcgtataa
     7381 tgcgcctccc gtaacgacgc agaaatgcga aaattacgaa agcaaaatta agtagta
//
LOCUS       DAJTUP010000159         7184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTUP010000159 DAJTUP010000000
VERSION     DAJTUP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.161"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..803
                     /locus_tag="OO468_003727"
                     /pseudo
     CDS             1..803
                     /locus_tag="OO468_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            808..1773
                     /gene="rbsC"
                     /locus_tag="OO468_003728"
     CDS             808..1773
                     /gene="rbsC"
                     /locus_tag="OO468_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR6260678.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            1798..2688
                     /gene="rbsB"
                     /locus_tag="OO468_003729"
                     /pseudo
     CDS             1798..2688
                     /gene="rbsB"
                     /locus_tag="OO468_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            2814..3743
                     /gene="rbsK"
                     /locus_tag="OO468_003730"
     CDS             2814..3743
                     /gene="rbsK"
                     /locus_tag="OO468_003730"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR6260679.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            3747..4739
                     /gene="rbsR"
                     /locus_tag="OO468_003731"
     CDS             3747..4739
                     /gene="rbsR"
                     /locus_tag="OO468_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR6260680.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(4705..6132)
                     /gene="mdtD"
                     /locus_tag="OO468_003732"
     CDS             complement(4705..6132)
                     /gene="mdtD"
                     /locus_tag="OO468_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR6260681.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6155..6847)
                     /locus_tag="OO468_003733"
     CDS             complement(6155..6847)
                     /locus_tag="OO468_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR6260682.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1821 a         1747 c         1900 g         1716 t
ORIGIN      
        1 gagatgatgg tgggccgcaa gctggaagat caatatccac acctggacaa agcgccagga
       61 gatatccgcc tgaaagtcga taatctctgc ggacctggcg ttaacgatgt ctcttttact
      121 ttacgcaaag gcgaaattct tggcgtctct ggtttgatgg gcgcaggtcg taccgaactg
      181 atgaaagtgc tctacggcgc actgccgcgc accagcggtt acgtcaccct ggatgggcat
      241 gaagtcgtta cccgttcacc gcaggatggc ctggcaaacg gcattgtgta tatctccgaa
      301 gaccgtaaac gtgacggttt agtgctgggc atgtcagtaa aagagaacat gtcgctgaca
      361 gcgctgcgct acttcagccg cgctggcggc agtttgaagc atgccgatga acagcaggct
      421 gtgagtgatt tcattcgtct gtttaatgtg aaaacgccgt cgatggaaca ggcaattggt
      481 ctgctttccg gtggcaatca gcaaaaagtg gcgattgccc gcggtctgat gacaccccca
      541 aagtattgat ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct
      601 atcaactgat taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga
      661 tgccagaagt attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg
      721 gggaatttac tcgtgagcag gccacccagg aagtgttaat ggctgtcgct gtgggcaagc
      781 ttaatcgcgt gaatcaggag taaaaaaatg ataacccaga ctgtctctgg tcgccgttat
      841 ttcacgaaag cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg
      901 attgtctcga cgttaagccc gaactttttc accatcaata acttattcaa tattctccag
      961 caaacttcgg tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc
     1021 atagacttgt cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc
     1081 ggcattgaag tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt
     1141 ggtgcggtaa ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg
     1201 gttatgatgc ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat
     1261 accggcttta ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc
     1321 gtaccgacgc cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat
     1381 cacacgcgtc tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt
     1441 tctggtatca acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca
     1501 tcgctggccg ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact
     1561 ggctatgagc tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga
     1621 aaaggtcgca ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga
     1681 ttgaatttgt taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg
     1741 ctggcggtgc tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg
     1801 aacatgaaaa aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg
     1861 aatgcgatgg caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt
     1921 gtatcgctga aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg
     1981 ctggactccc agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc
     2041 ggcaccaaaa ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag
     2101 atggctaact aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa
     2161 gtggtgagcc acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc
     2221 gcgaagaaag cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc
     2281 gcagcccgtg aacgtggcga aggcttccag ctggccgttg ctgctcacaa gtttaatgtt
     2341 cttgccagcc agccagcaga ttttgatcgc accaaaggtt tgaacgtaat gcagaacctg
     2401 ttgaccgctc atccggatgt tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc
     2461 gcgctgcgcg cactgcaaac tgccggtaaa tcggatgtga tggtcgtcgg atttgacggt
     2521 acaccggatg gcgaaaaagc ggtgaatgat ggcaaactag cagcgactat cgctcagcta
     2581 cccgatcaga ttggcgcgaa aggcgttgaa accgcagata aagtgctgaa aggcgagaaa
     2641 gttcaggcta agtatccggt tgatctgaaa ctggttgtta agcagtagtt ttaatcaggt
     2701 tgtatgacct gatggtgaca taaatacgtc atcgacagat gaacgtgtaa tataaagaaa
     2761 agcagggcac gcgccaccct aacacggtgg cgcattttat ggacatcccg aatatgcaaa
     2821 acgcaggcag cctcgttgtt cttggcagca ttaatgctga ccacattctt aatcttcaat
     2881 cttttcctac tccaggcgaa accgtaaccg gtaaccacta tcaggttgca tttggcggca
     2941 aaggcgcgaa tcaggctgtg gctgctgggc gtagcggtgc gaatatcgcg tttattgcct
     3001 gtactggcga tgacagcatt ggcgagagcg ttcgccagca gctcgccact gataacatcg
     3061 atattactcc ggtcagcgtg atcaaaggcg aatcaacagg tgtggcgctg atttttgtta
     3121 atggcgaagg tgagaatgtc atcggtattc atgccggcgc taatgctgct ctttccccat
     3181 cgctggtgga agcgcaacgt gagcgtattg ccaacgcgtc agcgttatta atgcagctgg
     3241 aatcaccact cgaaagtgtg atggcagcgg cgaaaatcgc ccatcaaaat aagactatcg
     3301 ttgcgcttaa cccggctccg gctcgcgaac ttcctgacga actgctggcg ctggtggaca
     3361 ttattacgcc aaacgaaacg gaagcagaaa agctcaccgg tattcgtgtt gaaaatgatg
     3421 aagatgcagc gaaggcggcg caggtactgc atgaaaaggg tatccgtact gtactgatta
     3481 ctttaggaag tcgtggtgta tgggctagcg tgaatggtga aggtcagcgc gttcctggat
     3541 tccgggtgca ggctgtcgat accattgctg ccggagatac ctttaacggc gcgttaatca
     3601 cggcgttgct ggaagaaaaa ccattgccag aggcgattcg gtttgcccat gctgccgctg
     3661 cgattgccgt aacacgtaaa ggcgcacaac cttccgtacc gtggcgtgaa gagatcgacg
     3721 catttttaga caggcagagg tgacgcttgg ctacaatgaa agatgttgcc cgtttggcgg
     3781 gcgtttctac ctcaacagtt tctcacgtta tcaataaaga tcgcttcgtc agtgaagcga
     3841 ttaccgccaa agttgaagcg gcgattaaag aactcaatta cgcgccatca gctctggcgc
     3901 gtagcctcaa actcaatcaa acacatacca ttggcatgtt gatcactgcc agtaccaatc
     3961 ctttctattc agaactggtg cgtggcgttg aacgcagctg cttcgaacgc ggttatagtc
     4021 tcgtcctttg caataccgaa ggcgatgaac agcggatgaa tcgcaatctg gaaacgctga
     4081 tgcaaaaacg cgttgatggc ttactgttac tgtgtaccga aacgcatcag ccttcgcgtg
     4141 aaatcatgca acgttatccg acagtgccta ccgtgatgat ggactgggct ccgttcgatg
     4201 gcgacagcga tcttattcag gataactcgt tgctgggcgg agacttagca acgcaatatc
     4261 tgatcgataa aggtcatacc cgtatcgcct gtattaccgg tccgctggat aaaactccgg
     4321 cgcgcctgcg gttggaaggt tatcgggcgg cgatgaaacg tgcgggtctc aacattcctg
     4381 atggctatga agtcactggt gattttgaat ttaacggcgg gtttgacgcc atgcgccaac
     4441 tgttatcaca tccgctgcgt cctcaggccg tctttaccgg aaatgacgct atggctgttg
     4501 gcgtttacca ggcgttatat caggcagagt tacaggttcc gcaggatatc gcggtgattg
     4561 gctatgacga tatcgaactg gcaagcttta tgacgccacc attaaccact atccaccaac
     4621 cgaaagatga actgggggag ctggcgattg atgtactcat ccatcggata acccagccga
     4681 cccttcagca acaacgatta caacttactc cgattctgat ggaacgcggt tcggcataga
     4741 tttacgctgt cttttgatca aattattacc atcggttgtt ttcagaagca tgaacattgc
     4801 tgctgaagca acagtaataa tgcccatcgt gataaacgta tagtggaatt gttcgacagt
     4861 cgttgtgcct tccatccctt cataaatgcg aaggacggcc gcacttacag caacgcctaa
     4921 actaatcgac agttgctgcg tgaccgccag aacactgtta ccgctgctgg cgttgtcatc
     4981 ggtcagatcg gcaagtgtga tggtattcat cgcggtaaac tgcgtcgaca tagccatccc
     5041 taatataaac aacggcaaga tcagcatcca tatcgccatt gccggtgatt gcaaagagaa
     5101 ctgagcgatc attagcccaa taatcaccgt gatccccact aatgtatggc gatagcccag
     5161 acgacgtaag acttgggtaa ccatcgattt tgcaataatg gaacctaacg ctgtcggtgc
     5221 catcatacag ccggcaataa acgcctgata accaaatcct acctgtaaca tcaatggcat
     5281 aaggaacggt acacagccgg tccccagacg ggttgcaata ttgcctacga taccgatcga
     5341 gaaagtgcgg gttttaaata aatctaatga aattaatggg tttggcgtac gtcgcgcatg
     5401 gagaatatag agaagcagta acccgatgct ggtgacaatt accgtcaagg caatccagct
     5461 ggcgacaatc ttttccccga atagctctat tccgcttgaa aagagaacaa ggctgaggcc
     5521 aaacagcaaa aagccagtga tatcgaatct gcgtcgtgcg gtggtgaaat tgggcatatg
     5581 tttgcgcgcg taaagaaggc ccgcaatacc tatggggata ttaattaaaa atatccagtg
     5641 ccaggttgcc caggtgacca gcacgccgcc aagaacgggg cctaaaattg gccccaccag
     5701 acccggcatg gcgacaaaat tcaataccgg aagaagttca ttacgaggat aagcacgcag
     5761 taaggccggc cgagcaacag gcatcatcat tgcgtcgcct atcccttgaa taacccggaa
     5821 gacaaccagc tgtggtagcg aattagaaag tgcgcaggcc agagaaccca acgtgaacag
     5881 actcacggca agggtaaaaa tgcgacgcgt accgaagcga tcggctagcc atccgcttac
     5941 cggaataagc atcgccaccg tcagcgtata actgatgatg gctgattgca tcgcgagagg
     6001 agaacgatta aggctatgag cgattgcggg taaggcggta ttcagaatag tggcatcaag
     6061 tgcctgcatg aagaaggcca tcgccgcgat ccacggcaaa cccgacatac tacgcttctt
     6121 tttatcgctc attcaatgtc ctgttatcgg gttatcactt atcaggtgag cgtagcagcg
     6181 cctgacaagc tttaaatgcc gcgtcgccat cgctttggat aatcgcatcg acaatcgcct
     6241 ggtgcagatc cagctttatc actgtgtcgc tggtaattga cgtgaagtaa gtgtgataaa
     6301 ccgaatggaa tagcgaggcg aatgaggtca aaaacggatt ggcgctcatt tcatagatat
     6361 gctcatgcca ggccatatcg acttcgatcc agcgttcacg gcgaaagttc tcttttaatg
     6421 ccgccatttc ggccattaac gtattgagat gcgccttctg ttccgcggtg ccaaccgttg
     6481 ctgccagtag gcaggcttgc ggctccagac agatacgcat aaccagaaag tgatcgatga
     6541 cctgatgaaa gttctcttct gttatccacc aagtaagcaa ttcctgatca agaaaattcc
     6601 agttcgattg tggcatgacc cgagtaccaa ttcgcggtcg cggtaaaacc atcccttttg
     6661 ccgttaacgt tttgaccgct tcgcgtaccg ctgtacgact cactccaaat tgctcgccca
     6721 gctcaatctc accgggcaaa atggtgccgg gttcatattc accttttaag atccgttgcg
     6781 ccagcttctc agccagaaca tacgaaaggt ttttctgtgc agctaactgt tgtgcgctta
     6841 aaggcattac ttatcttcct ttttcttttt attcctcctt agtatgccac caggaagtgt
     6901 gattacggtt gcaaaaacgg caaattgctt gttttatggc acattaacgg ggtgttttgc
     6961 tgaaaaaatg cgcggtcaga aaattatttt aaatttcctc ttgtcaggcc gggataactc
     7021 cctataatac gccaccactg acacggaaca acggcaaata cgccgccggg tcagcggggt
     7081 tctcctgaga attccgacag aaaaagcaaa aataaatgat tgactctgta gcgggaaagc
     7141 gtattatgca cacccccccc ccgcgccgct gagaaaaagc gaag
//
LOCUS       DAJTUP010000160         7108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTUP010000160 DAJTUP010000000
VERSION     DAJTUP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.162"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..107
                     /locus_tag="OO468_003734"
     CDS             <1..107
                     /locus_tag="OO468_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6260683.1"
                     /translation="DPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
     gene            complement(510..1091)
                     /locus_tag="OO468_003735"
     CDS             complement(510..1091)
                     /locus_tag="OO468_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260684.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(1138..4995)
                     /gene="sigA"
                     /locus_tag="OO468_003736"
     CDS             complement(1138..4995)
                     /gene="sigA"
                     /locus_tag="OO468_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR6260685.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5356..6360)
                     /locus_tag="OO468_003737"
                     /pseudo
     CDS             complement(5356..6360)
                     /locus_tag="OO468_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6345..6722
                     /locus_tag="OO468_003738"
                     /pseudo
     CDS             6345..6722
                     /locus_tag="OO468_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6761..>7108)
                     /locus_tag="OO468_003739"
     CDS             complement(6761..>7108)
                     /locus_tag="OO468_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6260686.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1765 a         1615 c         1609 g         2119 t
ORIGIN      
        1 tggatccaga aagctacctt cgccatgtgc ttggcgtcat agcagactgg ccggtcaacc
       61 gggtcagcga actgcttccg tggcgcatag cactgccagc tgaataacac atccccgtca
      121 atacggccct cgctgtacgc ttactcatca cctgctatta atccccgagt cagtacttcg
      181 cggcctgacg cgatcaccct actcagtact tcagagcccg cctccggtac ctgcctggcg
      241 atcgcccctg tgcaacaaaa taaatcaacc gttatcaaac cacgatcgtt cgtatgcgcc
      301 gccgatgacg cgcgcctgaa aaacgcgtca atatgagttc cccggaagct tacgataata
      361 cgcttatgaa cattacaaca cgctcatgcg gaacctggat gggggaagga acggtgaagc
      421 tgttagcgaa tgaccggcgt ttatacaaac gccggtcatg atgaatgata tttcagaata
      481 tacctcgggc taaaataccg ggacagagat caggcgtgat taatctgcag catttttaat
      541 aacagaagta ctgagcttac tttacctttg atgatgtaat agttatttcc gtcggtactc
      601 atttttccct gaaataatgt cacggatgtt tcttcctgct gatcctgcag gcgcagtcgt
      661 aaggtcatat ttttaatgag ctgagatccg ggtggcaagg ggtatttgct gtcagatact
      721 gggcgactga gtctgatgtg gcgcgtatac agcccgtttt cgtacagggt ggcctctgtt
      781 atggtgtcag tgaccagcat gtgggagaca atgctgtatt cttttttaaa gcacccacca
      841 gaaacggtga ttgtaatgat aatggtcttc tcgatcgaga tgtaaatacc cgtttcgtta
      901 tactgtccgt atcggaacca tgagaactca gttgcattcc agccatgctc cagaatatgt
      961 gcattttttt gcctgatagt ttcttttttt attcgctcat gtttaaggca taacaggcag
     1021 ataaataatg tcagaaaaag aaagctaata atgagagcaa gagcgatata gtctgtcaca
     1081 gggtaataca tattctctcc ggtagaagaa gggccgcaaa cgcggcccgg gctgttatca
     1141 gaaagagtaa cggaagttgg cgttgatcgc gttatccacg ttgtatttac caaatgccga
     1201 tttctcaaac tcaagaccga agcgaagatt atcgcgaatt tcggcgttga gaccaacatt
     1261 catgagaata cgaccgtctt tttcaccttt gatacgtttc tcaccggacg catcacgcag
     1321 tacggtttca ccgttggcaa acaggtcaaa ctggtagcca aggccggcgc gggctgtgac
     1381 tttccagtcc ttaccggaga aggatttacc cacatcaaca ccggtacgcc caatcagcgg
     1441 attaaagtcc ttatccttca tggtcacgct cattccgcga tcctgccagt cgaaccgttt
     1501 acctgatacg gccccataaa ccagttctgc ctgcggctca atccaggagt cttctgttac
     1561 atgatagcgg tagcctgctt cagcacccac atacaaggaa tgagaactgt agtcttttgt
     1621 tccgagtcca gcaaaggtcg ccgagtactc attatcatgg tgaacatact tactaatcag
     1681 gtcgatatag gcaccagagt caaatatagc ggaagcatac agacctgccc cgacggactt
     1741 cgtcttgcca ctgaatgcat tactgctggc atgactgtcg gtatacgtca tagtcagacc
     1801 agtgaaaagg tcaagtccat ccagctcatg ttttttatcc acaccaatct gcacatgggt
     1861 gtagttgtca ctgtatccac cagaagctga acctgctccg ctcatgatgc gggcccatgc
     1921 accggcctcg ccattggtgt cacgcagatc ccccattcgt ttattcaggt tgttgacttc
     1981 tgtcaggaag gctttgtaat taccggacat atacgatttt gctgcaccgg aagatgctgt
     2041 attttcagcc accttatatc cggtcagggt ccacgtggta tgttcctgat cctctttcgt
     2101 gctgatcaca ggagatatgt tgctgaaacc tatggcctga gtactggctt taaatatctc
     2161 tttattgctt ccttttgggg ctgttatcag gtcaacgttt aatcctgagt tattatttcc
     2221 cactttctgt atatcgaccg acagactgtt attgcttccg gttaactgat ttttaacaat
     2281 gagtttatcc gattgattta cagactggct cagatcagcc cgcataatca cagcagtatt
     2341 agtggcatcc agtgtgttta ctgtcacagt atggaaaccc gaattctcta tgttactaaa
     2401 caacaaaaga ctgtcgcggg tttttaatga ttgcagggct gactgtccgt ttactctcca
     2461 taaggtgtta tacatgctgg ctgaggctgc aggagcatca atacctccgg tataagaagt
     2521 atcaaagcca cccagcaaac ctgtagccag cggtgtgtgt gtggttttgt ccaggttatc
     2581 ttctttatac tggtcaacac caaacagaat agatgctttt ttgtctgatt ttatattacc
     2641 attgacccag gcataatttt gagcctttaa cgtggcacca tcttctgtca tattatatcc
     2701 atctgacata tagattgccg ggctgtattt attattttct gtaggggtgc ctgttaatgt
     2761 cagggttccg gccagttcaa tagctttatc tacatacatt tttccctggg cggtaacagt
     2821 agctcctttg ttgataatca ggtctgtatt aattaaggat aatgcaccag tcatagtaac
     2881 agttgattta tcaccaacag ttattttgct gttttgagca ctcagactgc cgttgaaact
     2941 actttcgcct gctgaatccc cggagcgaag ctgttgtctg aatccaaaac cttcgccagt
     3001 aatgtttctt cctgagaaca tgtcaatgta tgcagtttta tttcccagaa caatttgaga
     3061 gtttttagcc tctatgtgtc cttttacatt agcatcccgg cctatactca gggttgcgtt
     3121 ttctaaattt aatttccgga aattaaattt acgactttcc cagtcgggct gggaaaaaga
     3181 ggctatctga ttgttggact tatattccgt attgtaacgt ttatttacag tgctttcctg
     3241 gtcctttatg gcagcagaat agtctttttg acataaaaaa ggaattggac agttgttatt
     3301 gccttcttta aatatggcat gttctgtcgc atgtccttgt aatgttaact gtgcattccg
     3361 gacagagata tcgtttttga tatcgactga gccatcaaag atcagattgg cattattgtt
     3421 atgttgctgg gtagtattga taatatgatt tatgcttata ttacctgata cattcccatg
     3481 gtacatatat gcatcagtat tagtaagtga taatgttgat tgcttcacgt ttgagttcgt
     3541 tacggttgtt cccgcatctg ttgctgcgat tttctgaaat gactggtcat agccgtttag
     3601 atccagtgtt ccgccatttc tggtaaaata gatttcgcca ttaccgcttt cgcttaaagc
     3661 gtctttggca tttattttta ccgtaccttt acctccggcc atgtaaattt tattgaaggc
     3721 tttttcagca ctaaggatga cagtcccatt acctatttta aggttatttc cctgtgctat
     3781 ttttacatcc agagtaccac tacctatttt atgcagatta tctccggcaa cgcccttaac
     3841 attccattca acggtggtat tattatcaat atctatcccg gcacctttat aagacttatc
     3901 ttttccgtac actttatatg tctggttctt atcgaatata agcccgccaa tgccaagatc
     3961 ggtatcctgc ttgagcgtta atacccctcc tcctgagaca gacaggtcct tatcttttat
     4021 aatcgatgat aactcttgtt ttttaccatc cagctgcata gtgtttgtga aattcttttt
     4081 taaattgttt accagaatat tattaaataa cgagtagttt gtccaggcta tattggtact
     4141 actgataaaa ccaactgtac tggtaacacc atggaaaacc catttttgtt gttctttatc
     4201 atatagataa gaaccagaac cactatcgcc aaaaattggt gagtttttaa aaggggttct
     4261 gttggtataa atgtaactct tgtttttcca gtccagatta aaaagacttg cgcttaacaa
     4321 gtcgggatta tagttttgtc caaacagata ttgtttgccg tttaatatcg ttgaggttgt
     4381 tccggcacct gctctgaagc caattatttg ttccttaccg ttgtagttta cgccatatct
     4441 tttcatcgca tcttcgggag aaagtgagaa gtcggcactc tccgtaaccc ctgttgtttc
     4501 cacgacgaat ttattcagac gatgaaccga aaagtcgcca gatgatactc tgttacttgc
     4561 tttatatgct gtctgatccc atgactgtgt tgttattgca tgatgctgtg taccgttatg
     4621 agtcgcagta acactgtacg cacctccaat tgatgttgtt gcacctttgt tggaaacagc
     4681 agaaaaatca ggtacaggta gttctggaaa atgaaatatc tttccatctt tcatcattaa
     4741 ttgaacattt gttgcacccg gtctgaactc tcctctgttt tgagcaaggt caagataatc
     4801 gcgggtccat atattattta tatccagcat tgctgcaaat gctgtttcgc cataacttcc
     4861 atatattgag gataaaataa taccgaggat aactttcttt cttgatgtac cgacactaac
     4921 tttccgggtc agttcagaaa cagcaactaa tccacctgta atatgactat atttcagtga
     4981 ataaatttta ttcatacgtg aactccgata tttctgagtt gtggttatgg gatagcattt
     5041 aattacaacg gggaactatc agtcatggta cgtaactgat aagggtataa tgataatcac
     5101 tgtcagttat atttgtatga cagatgtaac aaaatgaaaa aaaagataag cacaagtgat
     5161 tgattggtta gatattgatc acttggtgtt tgtattttga attttgcgat caaaatttga
     5221 tctttgttgt ttccggtcaa tcctttacag gttttctgta ttatccgggg gagttaagtc
     5281 tgtcgatgtc cgacagactt ttgatgatgg aaacaacatg cagcgataat gtgcatgctg
     5341 ttttgcaggt ggaactcaga aggtgaactg gaactgtgcg accgcaccgt aagtgttgtc
     5401 cgtgccgatc aggcctgcaa catcaaggtg aaccacatca aacggtgaga tccccacccc
     5461 ggccgtgaat gcactgccgt cgttaccggc cagattctga cggtaaccgg cgcgcagctg
     5521 tgcccatttc caggcattaa attctgtgcc aatggcggca aactgacggt tgctgtcgga
     5581 ggtgaaaccg ctggccgggg tcagatccac atcaaatgca gtggtgaaca tcgcgttgtg
     5641 ccaggagaca ccggccgtca cctgcgggcg gatcctgaag gtttccgtga taccgttcac
     5701 ttcttttgtc tcaatactac gggagatcag gttctgtgcg accagtccca gcgtccagtt
     5761 gtcatccagg tccgtactgg caccgatgtc ggcgttgatg ccatttttcg tgttgtggta
     5821 gcggtcgcct ttgaaggcgc tgctgtcata atttctgacc agcacgttgt agttgaacag
     5881 gtcgacgcgc tggaatttgg gggggaagcc cagagaccat ttctgcccgg ccgtttccag
     5941 ttccgtagcc agagcaatac ccacatcagt gatcagtgcc gtgcgggcac aggcctgtga
     6001 tgtcaggcgg cttttgtcca cctcctgccc ggtgctgttt gccacctctt ccaggtaagt
     6061 gaggtcagca tcagagactt taccgtcgac actgacggtg ccgtgtgatt taaccatcag
     6121 tgcggcagag aggcgatcac cgggaagggc cgccacagca gaaacgccca gctgtgcggc
     6181 ggcatgtgtg ttacggaagt cccggaggtg ttctttcagg cgtgcggcag cctcaggaac
     6241 accgtggtta ctttcgatgg cgcggtcgaa ggccttccac tcatcactga tccggtcggc
     6301 gttgtcggtg atattatccg gattggagag ctgcgcgccg acggacggaa gcaggatgct
     6361 gggctcatgc gcgacgcaaa atccacgatg tatatatcag tactaaaagc gcgacggcgg
     6421 aagaagctct gaaactaatc ggtgaactgt acgccataga gcacgatata cgcgggttac
     6481 cggtgtctga acgcctggcg gtcaggcaaa tgcagagtag accgctcctg acttccctgt
     6541 ataagctgat gcaggagaaa gaacacacgt tatcgaagaa atgtcgtctg ggagatgcgt
     6601 tccggtatat caggaagcac tgggctgcgt tgtgcaactt ctgtgatgat ggcctggctg
     6661 aggtagataa taatgcagca gaaagagcgc ttcgtgcagt ctgtctcggg aagaaaactt
     6721 tttcgaggat cactcattcc gtgtctgact gcttcagggt ttaattttgc ttctgctgac
     6781 tgaatgatga ttgggcagca agtgcaatat cccacataaa tccagtcaac tcacgagcca
     6841 ccgccgtaat ggctacttgt gattttttcc cttttgctgt cagagttcgg taacgagaac
     6901 acaatcgttg ttgagctttc catgcaatat ctttggaata ttgtgttaca gagtccggtc
     6961 ggtaatatat aagccatgct ccaacttttg caggtgtacg ataagaccag gcggcttcgt
     7021 aaagcagacg tctcagttcg ctgtttccaa cttttgttat tcctctggga cgaatactgt
     7081 ttccactgga atattcacct ggcaccag
//
LOCUS       DAJTUP010000161         7079 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTUP010000161 DAJTUP010000000
VERSION     DAJTUP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.163"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..164
                     /locus_tag="OO468_003740"
     CDS             <1..164
                     /locus_tag="OO468_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6260687.1"
                     /translation="VFGNPVRGYPSMLVSILFLGGVQLIGIGVLGEYIGRIYLETKSR
                     PRYLIKSRK"
     gene            177..1442
                     /locus_tag="OO468_003741"
     CDS             177..1442
                     /locus_tag="OO468_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HCR6260688.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLSGLSGPFIIFIIASSVFKYLHLSKGVISIRGLYLFYTRLPYLAMILCG
                     LIQATSIILTFNGTRSHAPLGFSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYT
                     LSTLVITLCVFIYIKGNWQMKVFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRY
                     FVNIHIAIFSLICVCLFQCIKNKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQG
                     AELINKAKHGEAVSFTVLPPWLTLDLIKK"
     gene            complement(1509..2132)
                     /locus_tag="OO468_003742"
     CDS             complement(1509..2132)
                     /locus_tag="OO468_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6260689.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSADRPYWITGANCEVVDIGCVFTETT
                     SKATSASALSVTRSGKINANGSIDVATGVSSVNVVATGVYEVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLL"
     gene            complement(2883..3221)
                     /locus_tag="OO468_003743"
                     /pseudo
     CDS             complement(2883..3221)
                     /locus_tag="OO468_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head-binding protein"
     gene            3286..3744
                     /locus_tag="OO468_003744"
     CDS             3286..3744
                     /locus_tag="OO468_003744"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013043.1,HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6260690.1"
                     /translation="MEVLYYNKETGIFTWKKKLSARGAVGKVAGTISYGYNAINIDGV
                     RYFAHRLAWLYVYGEWPKQEIDHIDRNRRNNAISNLRDVSRVVNALNVGPRNSNAGIK
                     GVTFCQARNQWQAQINVSGKNITLGRFNTIDEAAIAYKAANMVADHLLSR"
     gene            3876..4136
                     /locus_tag="OO468_003745"
     CDS             3876..4136
                     /locus_tag="OO468_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HCR6260691.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQRAKMNNRSLNSEIIAAIEES
                     LDKQNSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSS"
     gene            complement(4226..6223)
                     /locus_tag="OO468_003746"
     CDS             complement(4226..6223)
                     /locus_tag="OO468_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6260692.1"
                     /translation="MAKAWKDVIASPQYQALAPEQKAQAQEQYFNEVVAPQAGENAEQ
                     AKQAFYAAYPLPSVQPVETQQPVAQQQPQQSGFMSDLGEAVKETGRGLVQAGVNVANI
                     PASVADAVTSAAAWAGGKLGIGDGTYQPAPRVTTHGLEQDFGLQQGALTPQTTEGRVF
                     AEALPYLTPAGVERAATQAPTLAGRIAQGATRLLAENAVGSLAANSAKDDAEALATDL
                     GVGVLAGGAINAAGRGLGAAYRGVRGAIAPEAQQAIRFAEREGVPLHTTDLLQPTSRV
                     GKMAQTTAENIPLAGTSGMRATQQEARSQLVQRFADKFGEYDPAVVIDSLKAKTSGIR
                     RAAGNRLEQVQNAMAGVNIQPARAIQQIDTEISNLQKLGKVADNETISKLQSYRDELV
                     RNAGPEGPVNLDLKQLSDLRSQFRMDVKGERPVLPNRSDAAIQRVYKAMTDDINGAIG
                     QNLGNDTLRKYQQANAVYADEAAKLKNTRLKNVLMKGDLTPEVVNNMLFSKNKSEIKT
                     LYNSVGRVGRVQMRNGIIGKAMEKSGGSPDQFLRQLNILQNQTGITFKGQDAAYLKGL
                     KNYLQSTQQAAKAAVTTPTGQQTIPFIIGYGTAMNPATTGAAVSYGLLTRAYESEPFR
                     NAMLRMANTPRGSTAFEKAMQQAQKAINALTQGAKSDALSE"
     gene            complement(6223..>7079)
                     /locus_tag="OO468_003747"
     CDS             complement(6223..>7079)
                     /locus_tag="OO468_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6260693.1"
                     /translation="ELTRVGVDPNNVAQMYQQNPSGFGEFVDHLGMAALGPIDYFNVQ
                     DKMAGREIDRGRLAETIRSNQAGEALTARGQDIQIRGQNISAQNAALSREIQRAELQE
                     KALDRQIARESNQLKLEELKQKQADVRQKAEIARADRQAAAQGAVDTFSTALDSLNEI
                     EQSPGLSKAVGIRSAFPTVPGSDAANFEARLDTFKAQTFLPMVQSLKGMGALSDAEGK
                     KLSDAVGALSPKMSEKAFRDSIGKIRNQLESKLSTVKKQFDYQEPVQNTPGQQSPAGS
                     NFSSLWGD"
BASE COUNT         1889 a         1629 c         1490 g         2071 t
ORIGIN      
        1 ttgtctttgg taacccagta cgcgggtatc cctccatgct tgtatcaata cttttcctgg
       61 gtggagtgca actgatcggg attggtgttc tcggtgaata tataggtaga atttatttag
      121 aaacaaaatc aagaccacgg tacttaatta aaagcagaaa ataacaggat gatcttatga
      181 agatattcat aatgcgcaat tggcacaaga tatcaatttt tatacttgca tttactttga
      241 tttggcttag acgcattgac attttgacaa atgcccagtt ttgggctgag gatgcagttt
      301 tttggtataa agatgcatac gaacaaggtt ttttcagttc actcataacc ccaagaaatg
      361 gttattttca aacagtatca acactcattg ttggtgcgac gacatttatt aatccaattt
      421 acgcaccatt attatcaaac ttttttggaa taataattag agctataatt atatggttct
      481 tatttaccga tagatttaaa ttcctaagca ccacatcaaa aatcttcatc tctgtatatt
      541 taatttgcat gcccgggctt gatgaagtcc aagccaatat aacaaatgct cactggtatt
      601 tatcattgta tgttgcaatg ataataatat ctgaagaatc caagtcaaaa ttatggaaag
      661 cacatgattt attttttata gtattatctg gattgagcgg tccatttatt atattcataa
      721 ttgcatcatc tgtttttaaa tatttacact tatcaaaagg agtaataagc ataagaggtt
      781 tatacttatt ttatactcga ctgccatatt tggcaatgat cttgtgtggg ctgatacagg
      841 caacatcaat aattcttaca tttaatggaa caagatcaca tgctccatta ggattcagtt
      901 ttgatgttat gtcatctata atatcatcta atgtatttct tttttcattt gtcccttgga
      961 atatcgctaa tgctggatgg gacaatcacc ttttatcgta cacactatca actttagtaa
     1021 ttacattatg tgttttcatt tatattaaag gaaattggca aatgaaagtg tttgccaccc
     1081 ttccaatact tattgtcgca ttttctatgg caaaacctca actggcagat tctgttcctc
     1141 aactgccaac acttgcaacc ggaaacgggt caaggtattt tgtaaatatt catattgcaa
     1201 ttttttcatt aatatgcgtt tgtttgtttc aatgcataaa gaataaaacc ttaaaaatat
     1261 tctttaaaat ctatgtttca gtcatcctta ttgcaatgat aaaattaaac ttttttataa
     1321 cccctcttcc tgacatgaac tggtctcaag gagcagaatt aataaataaa gcaaaacatg
     1381 gagaagccgt ctcatttaca gtgctccctc catggttaac tcttgattta ataaaaaaat
     1441 aaaacttcac attaataaat aacaatgaat ccgcctccct acgtattccc gtagggaggc
     1501 tgtcgtgttt atagtagcct ggtaatgcta aatgcaaacg aactagagat tcctgctccg
     1561 ctaagcgtat tgcgcagggt caccctaaat gtatctacgc caatataagt aacatcacaa
     1621 ctaactgaat caggcgcaga tataggtgtt atctgcacaa tataaccgct agcaactgct
     1681 gcgagcggat gagaaaagct aacctcatac acacctgtag caaccacgtt aaccgatgaa
     1741 acaccagtag caacgtcgat actaccattg gcgttaattt ttcccgagcg tgtgactgac
     1801 aatgcagaag ccgatgttgc cttactggtt gtttctgtaa aaacacaccc aatgtcaact
     1861 acctcgcaat tagctcctgt tatccagtat ggtcgatctg cacttggctg ataatcccct
     1921 gcgctctgga acgttgtgcc gttgagtatg actgtaactt ttcccccacc tgagcttgtt
     1981 acttgaatat tagtgtgcgt gtatcttgca ctgctcacgc gatgaaaatt tccaccgttt
     2041 ataaccacag ttacagggcg cgtcccagta ttatctatag caaggtctgc ccccccggca
     2101 ttagcctcaa agtatgggtt gtttaatacc aaagcgcacg aaccgttaag gccatttatg
     2161 gctaattgcg caccacctgt actggaatcc ccctgagtac catttccttc acaagtcagg
     2221 ctattgatag ttgtaccagc accccagcgg tccccgagga taccccattc actgttctga
     2281 ctgcacgtaa gcctatttgc agttatagcg ttcagttcag aatgatttac ggagttggta
     2341 gcacgccaac cgatacgatt actctgcaag tacacgttcg tcatgtcgct cgtgagcatc
     2401 gagtcagtgc gtaacccttc cccgtcaggg tggcggcaga taacaatgtt ttcaagacga
     2461 gtatgtgctt tattctgcac cagcataccg taacttccgg gattcccttt cagcgtcatg
     2521 ttgccgatgt agtcatatgc agccaatctc tgactgttaa acgctgcgtc acctagcatt
     2581 tttacgttaa aatcagtagc tctacattcc agtattgtct cagccatgtt ctcgcctgag
     2641 taaaaacagc ggacacccgg agcacctaca aagtcggcag ggtaatcatc ataattaagt
     2701 accagaggag tggtaacatc atacacccct tgctttattt ttaatgttcc accgaaaggc
     2761 cacgatgtaa gcgggtcttg atatatttca ttttttattg caaatagctc atcttgaaga
     2821 tttttcccgc tattagtgcc aatgtaagta gccccttcat cagattgaag taactgcttt
     2881 aactgatccg ggtcatactt cagcaaattc ggaaaataga actgttgcga accatacgca
     2941 tcatatacag ccattgaatg gccttgctca gttacaaatt tggcaatctg cccgttgtac
     3001 acaggatatc cggcagcatt gataacaatt ggctgagaaa cagggacgtg agatccgtct
     3061 tcattctcta cataaacctg aatctggttt tcaggattta ccgggtcagt gtcaattttc
     3121 ccgatataaa tttttccatt ggcatttgct ttaaacgagc gagctaaagt aaataactga
     3181 cttggttgtg aaacaacaac gttgtataat tgttctggca taataaacat tccggagaaa
     3241 ataatgagta agagacgtca cgaggtagta actcgggagc gccttatgga ggttctctat
     3301 tacaataaag aaacaggaat tttcacttgg aaaaagaaat taagcgccag aggtgctgtt
     3361 gggaaagttg ctggcacaat ttcttatgga tataacgcaa ttaatattga tggcgtaaga
     3421 tattttgcac atagacttgc ctggctgtat gtatatggcg aatggcccaa acaagagatt
     3481 gaccacattg atagaaacag aagaaataac gcaatctcaa accttaggga tgtcagcaga
     3541 gtcgtaaatg cattaaatgt cggacctcga aatagcaatg ctggaatcaa gggggtaact
     3601 ttctgtcaag ccagaaatca atggcaagct cagataaatg tctcaggtaa aaacataacc
     3661 cttgggagat tcaacaccat tgatgaagcg gctatagctt ataaggcagc aaacatggtt
     3721 gctgaccatc tgttgagtag gtgatatctg acatttcatt gctccagacg aatgatatga
     3781 tgcaaccatg atgtgattgc ataccgaaat ggtactattg agtatttatc cagtaagtta
     3841 cgatgccatt ccacccaatt ggtgaggcat caaggatgta cagcaaatac gacgaggcgc
     3901 agtttcactt gagacttccg catgaactcc acgcgaaaat taagcagcgt gcgaagatga
     3961 ataacaggtc tctgaactca gagataattg cagcgattga agaatcactg gataaacaaa
     4021 actctgcatc agtttacatt gacgatgcag agcgtatggc agaacaacaa tccgatatgg
     4081 ttaagaaaat tgtctttgat acgctcaaag agctatataa aaaagacagc agctaaccat
     4141 cagttacgga ggatttatgc aaagagatat gctgaatatt gcgttctaca tatttggttt
     4201 ttgcacgttc ctggtgtttg cgaagctatt ctgacaacgc atcagacttg gcaccctgag
     4261 tcagggcgtt aatggccttt tgcgcctgct gcatggcttt ctcaaacgct gttgatccgc
     4321 gtggggtatt tgccattcgg agcattgcat ttctgaatgg ctcgctctca taggcgcgag
     4381 taagaagtcc gtagcttacc gctgcgccag ttgtcgccgg gttcattgcc gtcccatacc
     4441 cgataatgaa cgggatggtt tgctgccctg ttggtgttgt tactgccgct tttgctgcct
     4501 gctgagtgga ctgaaggtaa tttttcaatc ctttcagata agcagcgtcc tgccccttaa
     4561 atgtgatgcc agtctggttt tgcaggatgt taagctgccg aaggaactgg tcaggggatc
     4621 cgccagattt ctccatcgcc tttccaatga tgccattgcg catttgcacc ctgccaacac
     4681 gaccaactga gttatacagc gtcttaattt ccgatttgtt cttgctgaat agcatgttgt
     4741 tgacaacttc cggcgtcagg tcgcctttca tgagaacatt cttcagcctg gtattcttta
     4801 gtttcgccgc ttcgtcagcg tagacggcat tggcctgctg atatttacgg agagtatcgt
     4861 tgccaagatt ctgaccaatg gcaccattga tatcgtcggt catcgccttg taaacgcgct
     4921 gaatggcagc atcggaacgg tttggtaaca ctggtcgctc acccttcacg tccattctga
     4981 actgactgcg caggtcgctt aattgcttca aatccagatt taccggacct tcagggccag
     5041 cattgcgaac aagctcatca cgatatgact gaagttttga aattgtctcg ttatcagcca
     5101 ccttaccaag cttctgcagg ttagatattt ctgtatcaat ctgctgaatt gctcgcgcag
     5161 gctgaatgtt tactccagcc atagcattct gaacctgctc cagtcgatta ccggcggcac
     5221 gacgaattcc tgatgttttc gctttaaggc tgtcaataac aaccgctgga tcatactccc
     5281 cgaatttatc agcaaatctc tgcaccaact ggcttctcgc ttcctgttgc gttgctctca
     5341 ttccgcttgt gccagccaga gggatatttt ctgctgtagt ctgcgccatt tttccgacgc
     5401 gggaagttgg ttgtaacagg tctgtggtgt gcagaggaac tccttcacgc tctgcaaatc
     5461 tgatagcctg ctgagcttct ggcgcgatag caccacgaac gccacgataa gcagcaccta
     5521 atccacgtcc ggcagcgtta atagcaccgc cagcaagtac accaacgcct aaatcggtgg
     5581 cgagtgcttc cgcatcatct ttcgcactat ttgcagcaag tgatccaact gcgttttcag
     5641 cgagaaggcg agttgcaccc tgagcaattc gaccagcaag tgttggtgcc tgtgttgccg
     5701 ctctctcaac gccggcagga gtgaggtaag gcaatgcttc agcaaatacc ctgccctctg
     5761 tcgtttgtgg agtcagcgca ccttgctgaa ggccaaagtc ctgctctaat ccatgcgttg
     5821 ttactcgtgg cgctggttga tatgttccat cgccaatgcc gagtttaccg ccagcccatg
     5881 cagccgcgct tgttacagca tcggcaaccg atgcaggtat gtttgccacg ttcacgccag
     5941 cctgcaccag tccgcgacca gtctctttta ctgcttcgcc aagatcagac ataaatccac
     6001 tttgctgtgg ttgttgctgt gctactggtt gctgtgtctc cactggctgc acagatggca
     6061 atggataggc agcatagaaa gcttgcttag cctgctctgc attttctccg gcttgcgggg
     6121 caacgacttc attgaagtat tgctcctgag cctgcgcttt ttgttctggt gctaacgcct
     6181 gatactgtgg agatgcgata acatctttcc atgctttagc cattaatcac cccatagtga
     6241 agaaaaatta ctgccagcag gagattgttg ccctggcgta ttctgcaccg gctcctggta
     6301 atcaaactgt tttttaacag tgctcaactt gctttcaagc tgatttcgaa tctttccgat
     6361 agagtcacga aaagcctttt cactcatttt gggacttaga gcaccaaccg catcggataa
     6421 ttttttaccc tcagcatctg aaagagcccc catccccttc agggactgca ccatagggag
     6481 gaaagtttga gctttaaagg tgtcgagtct tgcttcaaag ttagccgcat cagatccagg
     6541 aactgtcgga aacgctgagc gaataccaac tgcttttgaa agtccagggc tttgctctat
     6601 ctcgttgaga gaatcaagcg cagtgctgaa cgtatcaaca gcgccctgag cggcggcctg
     6661 tctgtcagca cgagctattt cagccttttg ccgaacatct gcctgtttct gctttagctc
     6721 ttcaagcttt aactgattgc tttctctggc tatctgtctg tccagagcct tttcttgtaa
     6781 ttctgctctt tgtatttcgc gggaaagagc agcattctgt gcgctgatgt tctggccacg
     6841 tatctggatg tcctgacctc gtgctgttag tgcctcgcca gcctgattgc tgcggattgt
     6901 ctctgccagc ctacctcggt caatctcacg accagccatc ttgtcctgaa cattgaagta
     6961 atcaatcgga ccaagagcag ccatcccaag gtgatcaaca aactcaccaa atcctgaagg
     7021 attctgctga tacatctgag caacgttgtt aggatcaacg ccgacgcgag tcagttcct
//
LOCUS       DAJTUP010000162         6941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTUP010000162 DAJTUP010000000
VERSION     DAJTUP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.164"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..397
                     /locus_tag="OO468_003748"
     CDS             <1..397
                     /locus_tag="OO468_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6260694.1"
                     /translation="SSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYKARL
                     KEAKLLASTGSTGDSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNNR
                     RLLERLGHIPPAEAEKAYYASIGNDDLAA"
     gene            562..4680
                     /gene="pic"
                     /locus_tag="OO468_003749"
     CDS             562..4680
                     /gene="pic"
                     /locus_tag="OO468_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR6260695.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4831..5073)
                     /locus_tag="OO468_003750"
                     /pseudo
     CDS             complement(4831..5073)
                     /locus_tag="OO468_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5112..6062)
                     /locus_tag="OO468_003751"
                     /pseudo
     CDS             complement(5112..6062)
                     /locus_tag="OO468_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6113..6688)
                     /locus_tag="OO468_003752"
                     /pseudo
     CDS             complement(6113..6688)
                     /locus_tag="OO468_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6692..6941
                     /locus_tag="OO468_003753"
                     /pseudo
     CDS             6692..6941
                     /locus_tag="OO468_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1761 a         1688 c         1831 g         1661 t
ORIGIN      
        1 ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt tgtgggcccg
       61 tcgtccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt cactggccta
      121 taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg gtgactcgta
      181 tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa tacaccgtaa
      241 gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg actggtataa
      301 caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag aaaaagctta
      361 ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa acactctcca
      421 ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat attttccttt
      481 ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta tatcccttgt
      541 aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg ccccgtcacc
      601 ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac atgccgaaga
      661 ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc tcagatatcc
      721 caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt cgccgaaaac
      781 aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga cggaaaactt
      841 gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc aaatggcgtt
      901 gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg atatcggagt
      961 gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa ccacccttct
     1021 attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc ctcagcggta
     1081 acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac cgctttttat
     1141 cgggtgggta gtggtacgca gtacactaag gacaaggacg gaaatttagt taaggttgcc
     1201 ggtggatatg cttttaaaac aggaggaacc acaggagttc ctctgatatc tgatgcaaca
     1261 atagtctcta atcccgggca aacttataat cctgtaaacg gccctttacc tgactatgga
     1321 gcccctgggg atagtggttc tcctttgttt gcttatgata aacaacaaaa aaaatgggtt
     1381 attgttgctg tattaagagc atatgcaggt attaatggtg ctacgaactg gtggaatgtc
     1441 ataccaacag attatctgaa ccaggttatg caggacgatt tcgatgcccc cgtagacttt
     1501 gtttccggac tgggccccct gaactggaca tacgacaaaa catcaggcac aggtaccctg
     1561 agccagggca gtaaaaactg gaccatgcac gggcagaaag acaatgacct caatgccggt
     1621 aaaaatctgg tattcagcgg gcagaatggt gcaattatcc tgaaagacag tgtgactcag
     1681 ggtgccggtt atctcgaatt taaagacagt tacaccgtat ctgctgaatc cggaaaaaca
     1741 tggacgggtg ccggcattat tactgacaag gggacgaatg taacctggaa ggtcaacggc
     1801 gttgccggtg acaacttgca taagctgggg gaaggaaccc tgaccataaa cggaacaggt
     1861 gtaaacccgg gaggactgaa aacgggagac ggtatcgttg tacttaacca gcaggcagac
     1921 actgcaggta atatccaggc cttcagttca gtgaacctcg ccagcggacg tccgaccgtg
     1981 gtgctcgggg atgcccgtca ggtcaatccg gataacattt catggggata ccggggaggt
     2041 aagcttgacc ttaatggtaa tgccgttacc ttcacccgac tgcaggctgc tgattacggg
     2101 gcggtgatta caaataatgc acagcaaaaa tcccagcttt tactggatct taaggctcag
     2161 gatacaaatg tcagtgaacc gacgattgga aatatatccc cctttggtgg taccggcact
     2221 ccaggaaacc tgtacagcat gatactcaac agccagaccc gcttctatat tctgaaatct
     2281 gccagctatg gtaacactct gtgggggaac agcctgaatg atccggctca gtgggagttt
     2341 gttggcatga acaaaaacaa agcagttcag acagtaaaag ataggatcct ggccgggcgg
     2401 gcaaaacaac ccgttatctt tcatggtcag ctgaccggga atatggatgt cgccattcca
     2461 caggtgccgg ggggaagaaa ggtaatcttt gatggtagcg tgaacctgcc ggaaggtacc
     2521 ctgagtcagg acagtggcac cctgatattc cagggacatc cggttatcca tgcctccatc
     2581 agtggcagtg caccggtcag cctgaaccag aaagactggg aaaaccgtca gtttacaatg
     2641 aaaacactgt cgctgaaaga cgctgacttc catctttcac gtaacgcctc gctgaacagt
     2701 gacattaagt cggataacag ccatatcaca ctgggaagtg acagggcatt tgtggataaa
     2761 aatgacggaa caggaaatta tgtcattccg gaggaaggta cctctgtccc ggacaccgtg
     2821 aatgacagga gccagtatga agggaatatt acgctgaacc ataactcagc cctggatatc
     2881 ggcagcaggt tcaccggggg gattgacgct tatgacagtg ccgtcagcat cacctctccg
     2941 gacgtcctgt tgacagcccc gggtgctttt gccggcagtt cactgacagt gcatgatggc
     3001 ggtcatctta cagcactgaa cggtcttttc agcgacgggc atattcaggc cggtaagaac
     3061 ggcaaaatca ccctgagcgg tacaccggtt aaagatacgg ctaatcagta tgcccctgct
     3121 gtatatctga cggacggata tgacctgacc ggcgataacg cagcactgga aattacccgt
     3181 ggagcacatg cttccggtga tattcatgcc tctgcggcat caacagttac catcgggtct
     3241 gacacgccgg cagaactggc ttctgcggaa acggctgcat cggcgtttgc cggcagtctt
     3301 cttgagggct ataacgcagc attcaatggt gccataaccg gtggcagggc tgatgtcagt
     3361 atgcataatg cactgtggac tctgggtggg gactctgcca tccacagtct taccgtcaga
     3421 aacagccgta ttagttctga aggagaccgt acattccgta ccctgacggt gaataaactg
     3481 gatgcaacag gcagtgattt tgttttgcgt acggacctga aaaatgccga taaaattaat
     3541 gtgactgaaa aagccactgg ttcagataac agcctgaacg tcagctttat gaataatcct
     3601 gctcagggac aggccctgaa tattcctctg gtcacggcac cggcgggaac ttcagcagag
     3661 atgtttaagg ccggcacccg ggtgacaggt ttcagtcggg tgaccccaac cctgcatgtt
     3721 gataccagtg gtggcaatac gaagtggata ctggatggtt ttaaagcgga ggctgataaa
     3781 gccgctgccg cgaaggctga cagtttcatg aatgccgggt ataaaaactt catgacggaa
     3841 gttaacaatc tgaacaaacg tatgggtgac ctgcgtgaca caaacggtga tgccggtgcc
     3901 tgggcgcgca tcatgagtgg tgccggttct gcagacggtg gttacagtga taattacacc
     3961 catgttcagg tcggctttga caaaaaacat gaactggacg gtgtggacct gtttaccggt
     4021 gtcacgatga cctataccga cagcagtgca gacagccatg cattcagcgg aaagacgaaa
     4081 tcggtggggg gcggtctgta tgcttcagca ttgtttgagt ccggtgccta tatcgatttg
     4141 attggtaaat atattcacca tgacaatgat tacacaggta actttgctag cctgggaacg
     4201 aaacactaca acacccattc ctggtatgcc ggtgctgaaa cgggttaccg ctatcacctg
     4261 acagaggaca cgttcattga gccgcaggct gaactggttt acggcgccgt gtccgggaaa
     4321 acattccgct ggaaagacgg tgatatggac ctgagcatga agaacaggga cttcagtccg
     4381 ctggttggaa gaacaggggt tgaactgggc aagaccttca gtggtaagga ctggagtgtg
     4441 acggcccgtg ccggaaccag ctggcagttt gacctgctga ataatggaga gaccgtactg
     4501 cgtgatgcgt ccggggagaa acggataaaa ggagagaagg acagccggat gctgtttaat
     4561 gttggtatga atgcgcagat aaaggacaat atgcgctttg gtctggagtt tgagaagtca
     4621 gcctttggta aatataacgt ggataatgcg gtaaacgcga atttccggta tatgttctga
     4681 tgtgggggta aagggctgcc ggaaggcagc ctttctcaga gactcttaaa atataaaact
     4741 gaaggtcttc ggtaaggggg agtgtattag acgtcaacta gttttgccga ctggcgcgag
     4801 cctgtttaag attctgtgta aatgcctttc tcagaagtga ccgtccaggc ggtcaccgaa
     4861 ctcgataata aagcggctca ttgccattcg ccagtccttc aacggcatcg tccatttctg
     4921 ggacgcagac tggattgcca gccacaccac ttttttcacc gagtcgtctg tcgggaacac
     4981 tttacgcttt ttgatcgcat ggcggatcac gctgtttagc gactcgatgg cattcgtcgt
     5041 atagatcact ttgcggatgt ccgttggata agcggtaagc gtacagcgag ggccgtattg
     5101 acggggatgt gttattcagc tggcagtgct atgcgccacg gaagcagttc gctgacccgg
     5161 ttgaccggcc agtctgctat gacgccaagc acatggcgaa ggtagctttc tggatccacg
     5221 tcattcagtt tgcacgtccc gatcaggctg tacagtagcg cgccccgctc accaccatgg
     5281 tcagagccga agaacaggaa gtttttacga cccagactga ccgcccgcag ggcattttca
     5341 gcgatgttgt tgtcgatttc cacccagcca ttactgcagt acacgttcag ggccttccac
     5401 tgtttcagta ggtatgcgaa cgcttttgcc gtatccgagt gacgcgacag tgttttcatc
     5461 tgttgctgta tccggtcata cagtgactgc atcagtggcg cggctcttgc ttttcttgcc
     5521 gccagacgct gttctgctga acatccccgg acttctgcct cgatggtata cagttcaccg
     5581 atacgctgca gggcttccgt ggtgatgtcg gttggcactc ttgcatgcac atcgtggatt
     5641 tttctccggg catgggccat acacgcagct tccgttattc tgccggattc gtataacgcc
     5701 cggtaaccac cgtaagcatc tgcctgaagc acaccgctgt aaccggctag gtgattctgt
     5761 ggatggatac ctttccggtc cgggctgtac gcgaaccaga ccgccggggg catctgtgaa
     5821 ccggcgttac ggtcatcacg gacatagacc cacagccggg cagttcttgt tttaccactg
     5881 cccggctcct ggaccggaac ggggatatca tccgcatgaa ctttaccggg catcagtaca
     5941 tactggcgca ggacgtcata cagcggctcc agcagttcag caacggcacc ggtccagcga
     6001 cccagtgtgg cacggctcag ctccacgccc tgacgacggt atatttctga ctggcggtat
     6061 aactgtcaac gacggatgaa aagtggctct tccgcaacct gggtggagat tttcatgcca
     6121 gtggactcat taccctctgc ctgagtagtt caaatcctgc tcgaccatac atctggcgtt
     6181 tcagcatctt cttcaggcga tttacatgac cttcgacaac accattactc cagcgactac
     6241 tgattgcctc acatatagct gcagcatcag cttccatccc cgccgccacg cgccgaaatt
     6301 ctgctgaacc gctttcgtgg actcgagtga accaactgct tagctgtgat ttattttggg
     6361 ttttcagaat acggtagaac tcgagtgcca gttgctgcgc tattttcagc tcaggttctt
     6421 tttcacacat cagactaata aatcgggaag cataattttc ttcatccctg gttattctcc
     6481 agggcatcaa ccagcggctt acccgggaga ctgaaggaag tctgacggca gtagtaaccg
     6541 gtgggttcca gcctttacgc catttggcaa ccgcatccct gacgatggtt tcactgcctg
     6601 taaatccccg ggccaccatt tcccgccata tccggctggc gttataatta ccactttctc
     6661 gttgctcttt taaccactcc ctccatggtg tcagcgaaaa acgaaaccgc tgttgaaatc
     6721 cctggaaagc tggttgcgga aaagatgaag acccagtcgc gacactcaga gttggcgaag
     6781 gcgttcgcgt acgcacttaa ccagtggccg gcactgacgt actatgcgaa cgatggctgg
     6841 gtggaaatcg acaacaacat cgctgaaaat gccttgcggg cggtcagtct gggtcgtaaa
     6901 aacttcctgt tcttcggctc tgaccatggt ggtgagcggg g
//
LOCUS       DAJTUP010000163         6913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTUP010000163 DAJTUP010000000
VERSION     DAJTUP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.165"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..685
                     /locus_tag="OO468_003754"
     CDS             <1..685
                     /locus_tag="OO468_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR6260696.1"
                     /translation="AICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKTTNQ
                     IHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELES
                     ELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYST
                     GGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVVACA
                     LANKLARIA"
     gene            complement(1024..1794)
                     /locus_tag="OO468_003755"
     CDS             complement(1024..1794)
                     /locus_tag="OO468_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260697.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1839..2273)
                     /locus_tag="OO468_003756"
                     /pseudo
     CDS             complement(1839..2273)
                     /locus_tag="OO468_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2285..2506)
                     /locus_tag="OO468_003757"
     CDS             complement(2285..2506)
                     /locus_tag="OO468_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260698.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2507..3190)
                     /locus_tag="OO468_003758"
     CDS             complement(2507..3190)
                     /locus_tag="OO468_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR6260699.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3254..3565)
                     /locus_tag="OO468_003759"
     CDS             complement(3254..3565)
                     /locus_tag="OO468_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260700.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3562..4464)
                     /locus_tag="OO468_003760"
     CDS             complement(3562..4464)
                     /locus_tag="OO468_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR6260701.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4904..5863
                     /locus_tag="OO468_003761"
     CDS             4904..5863
                     /locus_tag="OO468_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR6260702.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5863..6258
                     /locus_tag="OO468_003762"
     CDS             5863..6258
                     /locus_tag="OO468_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR6260703.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6441..6821
                     /locus_tag="OO468_003763"
     CDS             6441..6821
                     /locus_tag="OO468_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR6260704.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1889 a         1790 c         1673 g         1561 t
ORIGIN      
        1 agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa gaactgagac
       61 ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag ataaggtgaa
      121 aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc cgacagggga
      181 tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg aatatctgag
      241 ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa tagcagagct
      301 ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca ttatgacaat
      361 tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg acgggaaaca
      421 gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc agtatagcac
      481 tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt tacgccggtt
      541 actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg gaaaacttgc
      601 tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct gcgctctggc
      661 aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat atcaggcctg
      721 atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata caagttaccg
      781 tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct gattaaaaac
      841 ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc aggtgcggaa
      901 ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta agcaagccca
      961 ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc cccgccatcg
     1021 tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg cccgggcttc
     1081 ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt catccggtat
     1141 acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat cgctgaccag
     1201 atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga gcaccacggg
     1261 caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca cgaccaggcg
     1321 aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg ccgcccggat
     1381 acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc gcaccgcatc
     1441 aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga aagacacctg
     1501 cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca gaacaagttc
     1561 cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca gcacgccatc
     1621 gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt aaatgctgat
     1681 ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc agccctgcgc
     1741 cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat tcataattca
     1801 tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc gtgtgtgttg
     1861 tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac gtattccggg
     1921 agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg ttcagcgatc
     1981 tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc gcgcacgttg
     2041 ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta gtcttccggg
     2101 atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga aatatccatt
     2161 ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag cggcgtgtct
     2221 cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta catactctct
     2281 cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc tccatcaaga
     2341 ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg tcagcattaa
     2401 agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg gcattggcca
     2461 cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac gcagcctccg
     2521 gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg gcagccagtc
     2581 gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac cggcgtccgg
     2641 actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc actatcgcat
     2701 tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt tccgtcgggt
     2761 gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt tgtggcagac
     2821 gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca tatgcggcct
     2881 tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc gcatttttcc
     2941 aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc agcgcgtctt
     3001 ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca gttttatcac
     3061 cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga tcggtgagga
     3121 tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta ccgaggacaa
     3181 aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc gttccccgct
     3241 gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa taccgcagcc
     3301 gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt tcatcagaaa
     3361 agggcgacgg gctggcgtac actgtcaccg acaccagccg ggagcggtaa aagcgcccgt
     3421 cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc gccgcgtcag
     3481 ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa cgggcggttg
     3541 cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga ctatccacgc
     3601 aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt caagttcccc
     3661 ccacagcaca ttgacgagct ctccacgctc atacaactgc cagccacaga aatcacagcc
     3721 ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt ctgcaataac
     3781 gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt tttctccctg
     3841 actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt ccgtatacca
     3901 gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca cttcaacatc
     3961 caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg gcactacgtt
     4021 gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt cacaccagtc
     4081 acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg gtaatccttc
     4141 ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca ggcagttttg
     4201 ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg cgatattttc
     4261 cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag agcggatatt
     4321 ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac cttcatttgt
     4381 ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct cagcgatctg
     4441 tgccggctca ccaggaaaat acatacgatt cgagcaccag tttggcatgg gaattctcct
     4501 ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg catacgaatg
     4561 caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg accgggtgag
     4621 gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg ccggcacggc
     4681 ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat aagaactcaa
     4741 aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt gacatagact
     4801 caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga cataaaggat
     4861 gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa aagtatcatg
     4921 cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg taagaaccag
     4981 tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg ccggcaaagt
     5041 ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca ctgctgtcat
     5101 aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca tacatcatgc
     5161 actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat tacctgtgac
     5221 cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa aaaaggcaaa
     5281 tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa aagttaatgt
     5341 aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag taaacaaact
     5401 tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt taattctcgg
     5461 tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat cccgcatcac
     5521 tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt ttattgctga
     5581 tgagttaata aaaaacagac acgataatga atatctccag acattaataa atgatgtagc
     5641 tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg cagaaagcat
     5701 caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg gcggaggagc
     5761 cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga acaaggtgga
     5821 tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag cagtgatcca
     5881 aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca ggacaacctg
     5941 acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta cagacaatcc
     6001 cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct aatcagcgtt
     6061 tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca aacaacagga
     6121 tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc atcttcagaa
     6181 aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag acgcaatctt
     6241 tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc tcttctttta
     6301 tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa tgcaacagac
     6361 aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca ttattgtcag
     6421 tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt gatgatgata
     6481 taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact caggctattg
     6541 cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc acctcaattg
     6601 taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa gctcttgcag
     6661 tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa gcaaagctaa
     6721 tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa ataggtcttc
     6781 tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag ttatagtaac
     6841 tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat gaggcatgga
     6901 ccatcatcaa gcc
//
LOCUS       DAJTUP010000164         6789 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTUP010000164 DAJTUP010000000
VERSION     DAJTUP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.166"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            90..1514
                     /gene="sbcB"
                     /locus_tag="OO468_003764"
     CDS             90..1514
                     /gene="sbcB"
                     /locus_tag="OO468_003764"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR6260705.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(1557..1784)
                     /locus_tag="OO468_003765"
     CDS             complement(1557..1784)
                     /locus_tag="OO468_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR6260706.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1798..2856)
                     /gene="yeeE"
                     /locus_tag="OO468_003766"
     CDS             complement(1798..2856)
                     /gene="yeeE"
                     /locus_tag="OO468_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR6260707.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3035..4393)
                     /gene="plaP"
                     /locus_tag="OO468_003767"
     CDS             complement(3035..4393)
                     /gene="plaP"
                     /locus_tag="OO468_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR6260708.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4383..4445)
                     /gene="yoeI"
                     /locus_tag="OO468_003768"
     CDS             complement(4383..4445)
                     /gene="yoeI"
                     /locus_tag="OO468_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR6260709.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4606..5589)
                     /locus_tag="OO468_003769"
     CDS             complement(4606..5589)
                     /locus_tag="OO468_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR6260710.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5635..6459)
                     /locus_tag="OO468_003770"
     CDS             complement(5635..6459)
                     /locus_tag="OO468_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR6260711.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6542..6787)
                     /gene="yoeB"
                     /locus_tag="OO468_003771"
     CDS             complement(6542..6787)
                     /gene="yoeB"
                     /locus_tag="OO468_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003850603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR6260712.1"
                     /translation="MWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEP
                     LKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1836 a         1779 c         1593 g         1581 t
ORIGIN      
        1 tgacgttcgt agacatactc aggtgaaaaa agggttgctt ttcgagttat aacagacagt
       61 taattaacta cagtggacaa tgctagacaa tgacagacac agataaacaa cctaccttcc
      121 tctttcacga ttacgaaacc tttggcacgc accccgcgtt agatcgccct gcacagttcg
      181 cagccattcg caccgatgac gaattcaatg tcatcggcga acccgaagtc ttttactgca
      241 agcccgcgga tgactattta ccccggcctg gagcagtatt aattaccggt attaccccgc
      301 aggaggcacg ggcgaaagga gaaaacgaag ccgcgtttgc cgcccgtatt cactcgcttt
      361 ttaccgtacc gaagacctgt attctgggct acaacaatgt gcgtttcgac gacgaagtca
      421 cacgcaacgt tttttatcgt aatttctacg atccttacgc ctggagctgg cagcatgata
      481 actcgcgctg ggatttactg gatgttatgc gtgcctgtta tgccctgcgc ccggaaggaa
      541 taaactggcc tgaaaatgat gacggtctac cgagctttcg ccttgagcat ttaaccaaag
      601 cgaatggtat tgaacatagc aacgcccacg atgcgatggc tgatgtgtac gccactattg
      661 cgatggcgaa actggtaaaa acgcgtcagc cacgcctgtt tgattatctc tttacccatc
      721 gtaataaaca caaactgatg gcgttgattg atgttccgca gatgaaaccc ctggctcacg
      781 tttccggaat gtttggggca tggcgtggca ataccagctg ggtggcaccg ctggcgtggc
      841 atcctgaaaa tcgcaatgcc gtaattatgg cggatttggc aggagacatt tcgccattac
      901 tggagctgga tatcgacaca ttgcgcgagc gtttatatac cgcaaaagcc gatcttggcg
      961 ataacgccgc cgttccggtt aagctggtgc atatcaataa atgtccggta ctggcccagg
     1021 cgaatacgct acgcccggaa gatgccgacc gactgggaat taatcgtcag cattgcctcg
     1081 ataacctaaa aattctgcgt gaaaatccgc aagtgcgtga aaaagtggta gcgatattcg
     1141 cggaagccga accgtttaca ccttcagata atgtggatgc acagctttat aatggctttt
     1201 tcagtgacgc cgatcgtgca gcaatgaaaa ttgtgctgga aaccgagccg cgtaatttac
     1261 cggcactgga tatcactttt gttgataaac gaattgaaaa gttgttgttc aattatcggg
     1321 cacgcaactt cccggggacg ctggattatg ccgagcagca acgctggctg gagcaccgcc
     1381 accaggtctt cacgccagag tttttgcagg gttatgctga tgaattgcag atgctggtac
     1441 aacaatatgc cgatgacaaa gagaaagtgg cgctgttaaa agcactttgg cagtacgcgg
     1501 aagagatcgt ctaatttgaa gccgccgccg ctggtacggc ggaggacaat gttgagtcag
     1561 gctttttgaa cggtgatgct ccacgctgca tcgccaatct gctgataatc ggtgatggca
     1621 tgtccttctt ctgccgccca ctgtgggatt gcttcagttg cctgggtgca atcaaactca
     1681 attaccaact catcgccgct ggccatttct gccagtgctg cttttgcttc tatgagtgga
     1741 aacggacaga cctgcgtcac cacatccagc tttttaatcg ccatctttac accttactta
     1801 atttgccgca gcagttgcca gtcgcgcctt acgctgcggt cgaacataca caagccagga
     1861 cgcagtccag actccgagaa tcataaatac aaggccaatc cagccctgcc aggtcatcat
     1921 tgcagtcata accagtccat taccgataga gcaaccacct gcaatactgg caccgaagcc
     1981 catcagtaca ccacctaacc cgctacgtaa tgttgtttgt gcatcagctg cgcgaacgcg
     2041 gaactcacgg ctcgctttgg ctgcaataaa tgcctccacg aagatcccta acaccaggaa
     2101 aacgccccag ttaatgtatt tcacgtcacc cgcgaccaga aattgaagaa tattggccgt
     2161 tggggaagtg actcctaacc caaacatgcg tccggttgct tcactcaggg gccaggctaa
     2221 aagcgcaatc aaaccgatga gtacagcagt gacaaaggga tgccagcgtt tttcaaacag
     2281 aatatgagcg atcccggttc ggcgcggcgg taaggtcgcg actttgagtt ttggcttctt
     2341 caactctttc atcaccaccc agagcgttat caccagcaaa acggtaacca acggccacac
     2401 agacaaatta aaagtctcag caatagagtt atgttcagta gtgtagtgct gcaaggtttg
     2461 atttaaacca ctggcgtgtg gagaacgcat caccgcactc atcaccatat aagtgaaaag
     2521 cgcgatccaa ctgccgatca atccctcacc cgcgcgatac caggtcccgg tggcacatcc
     2581 acccgccaga acaattccca gcccgaaaag atacccacct ataacagtac caagccacgg
     2641 gaacgccccg gcttcgtaag tcagtaggcc cgcctgaatt aacgcaaaaa cccccacgct
     2701 ttgtgccgaa attgcaatca gcaaggcgta aaacatgcga ttatttttcg cgatatacat
     2761 atcgcgaaaa ccaccagtca ggcaaaaacg cccacgctgc atgacaaatc ccagcagagc
     2821 accacaaatt agcccgctta atatcattga aaacataaat taattaacca gatgaatgtt
     2881 aatggggaaa tttttcatga ctggtggaat gcagaccaat aaccaaattc tctaaattag
     2941 aacaaatggt tattaatgag gatttcgata aaaaagaata aaaaaaccgg agccaaatgt
     3001 tcattcgact ccggtttatt attagaaaag atgattatgc tacgtcttcg tactgcggaa
     3061 ccggattacg gaagctcttg gtgacgcaag ccaggtaaat cagaccgata gcagcccaga
     3121 tcagacccag aaccattgag ctttcttcca ggttaaccca cagcgcacca acggtcagcg
     3181 caccacacat cggcaggaac agatactgga agtgatcttt cagcgtcttg ttacgcttct
     3241 cacggatcca gaactgcgag atgaccgaca ggttaacaaa ggtgaacgcc accagcgcac
     3301 caaagttaat cagcgccgtt gccattacca ggtcgaagtt gattgccagc agggcaatcg
     3361 cgccaaccag gatgatgttc atcgccggag tacgccattt cgggtgtaca taaccgaaga
     3421 agcttttcgg gaacacgccg tcacgaccca ttacgtacat cagacgcgct acgcctgcgt
     3481 gcgccgccat accggacgcc agtacggtaa tggtggagaa gatcagcgcg ccaacctgga
     3541 acgctttacc cgcaacgtac agcatgattt caggctgtga cgcatccgga tctttaaagc
     3601 gagagatatc cgggaagtac agctgcagga agtaagttgc aaagatgaag atcatgccgc
     3661 caatcagcgc ggtcaggaaa atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt
     3721 ccgacagatt gctgatgccg tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg
     3781 taatcatcgg gataacatgc gcatcgccag accagaacgg acgcgtgctc gccagcgtac
     3841 cagcgccttc accttcaaat acgccataaa caaccatgcc cagaatcacc gcgatcagca
     3901 ctacctgcaa cacgacgatt acagtgttga cgttcgctac ggatttcaga ctacgcaggt
     3961 taaaggcggt cataaaggcc accagcgcca ccacaaacat ccacgatgga atggaaggca
     4021 ccagagcttc aaaatagatt ttcgccagca gaatgttgat catcggcgcg aacagatagt
     4081 cgagcagaga agaccaaccc accataaagc cgacagtcgg gctaatggat ttctgggcgt
     4141 aagtgtatgc agagccagca gaaggatagc ggcgaaccag cttcccgtag ctcagagccg
     4201 taaacaggat cgcaatcaat gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc
     4261 ctgaaacgat accaaatgta tcaaacagcg tcatcggctg catataggct agacccatca
     4321 ttacaaccgg aactaacgta agcgttttac gcaattccac gcgagaggtg tttggagtaa
     4381 cgttatgcga catggttatt ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc
     4441 cccatttttt tggattcctc agcgacaaca actgtcgatt tttagtaaat atctatccgg
     4501 tacgaagccc ggcctcttgg tatgaatgat tggtttgaag caaaaaataa ccgacgctaa
     4561 ttaaacgtcg gtttttaatc attttttgac agcggcgcat tgtgcctaaa tgggggggaa
     4621 atgacaagag aatgagaggc ttgtcataat aatttttctt tgaatggctg attttccgtc
     4681 atcagatttt gcataaacac cggcgatcgc actatttgct aaaatttcat ctcgccacca
     4741 gccgctggca agccctgcgg tctgttcatt ccagccaatc cagacaggtt caaagagagt
     4801 ttgggcgacc actttcttct cgattaacgc gccactatcg agaaaacgtt gcgccagata
     4861 ccgtggtaaa tagccgcagc caaggccgct aatttgcagc tccagtttgg ttttaaaatc
     4921 gaaaacggta atggcttctt gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg
     4981 cgcagtatcc cccaccacaa tcgcccgata tcgtttaatg atacgacggt ttaacggttc
     5041 ttcttcctgg gctaacggat gatgaggcgc gaccgcaaag acttgttcaa gatcgcccaa
     5101 ccgcgagaaa ccaaactcac tggaagatgg tggctcatgc atcgcgccaa cgataatatc
     5161 cgctcgcccc tgagtcaggg catcccagga accaccgagt acgccattga taaacttcag
     5221 gcgcgtcacg ctatgatgtt gataaaaagc ttcaatgaga ggcgcaagca gagaaaaagg
     5281 aaacgtgtcg tccacgccaa tcaccagctc gttttcccag ccttcatgca gtttgatcgc
     5341 ctgcttttcc agttctcgca cggtatgcag aacttcccgc cctttctcta ataacatttt
     5401 tccggtgcgg gtgaatttag cgcggtgtcc gctacgatca agcaattgaa tatttaggtc
     5461 gctttccagc ttgtgaacgg tgtaactcag agcggacggc gtcttgtaga gtttggctga
     5521 cgccgccgca aaactgccct ctttttctat ggcatcgagg atcatcaaca catccagcag
     5581 tggtttcata cacgccccct tgcagtgcat gcggcgtcct gctggcgtga tcgtttactc
     5641 caacggcatt accagcggat cgggatactg gtattcaaat cccagttcat tacaaatccg
     5701 actgccatca ataatcttac ctttgccgct gtccagacta tttctgaact gcggaggttg
     5761 cagccccagt aaacgggcca tctgcggata gaaaacatta cgcgcagggt gagctggcgc
     5821 acatatatta tagatgtgtc cgcctttagg tgcctgtaac aacagagtga tagcgccaat
     5881 aacatcttct aaatggacta aattaacacc atgttcacca tcaggggcgg tttttccggc
     5941 aaagaagcgt cccggatgac gtcccggtcc gaccaggccc gcaagacgca gaatatcgac
     6001 cgaagtaccg ggtaaattgt gcagccagtc ttcgagttct tctaatactc gtccactgtt
     6061 ggttaccgga ttacgcgggg tagtttcttt aaccgtgcct tgcgcgtcgc catagacaga
     6121 tgttgagctg gtaaaaataa ttcggggaat acgatgggcc agcgcgctat ccactaactc
     6181 ttgtaccgct tgtaaataga actcatcgcc ggggccgcta cgacgtgccg gaagcgtaat
     6241 gaccagcgca tcggcatcca taagggcatc cagatcgtca gaatcgcaaa ctaactcagg
     6301 ctccatgcga agcagatagc tatcaatgcc actcattcgg gccgcttcga caccatcttg
     6361 tgtggtttta ctcccggtga cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat
     6421 acctaaccac cctaacccga caattgcgac ctttttcatc cgttatctcc tgatacacct
     6481 tagatctata aggctacgct agcgtatcaa aactgacaat tcattctatg aatgaatctg
     6541 ttcaataatg ataacgacat gctgcaatga gcagtgaatc atcagtaacc gcgtatacca
     6601 gacggtgctc ctctgtaatg cgtcgggacc agaaacctga caaattatgt ttcaggggtt
     6661 ctggtttccc cttaccttca aatggtgttc tgcgggtatc tttgataagt tcattgatct
     6721 ttttaacaat tcgcttatct gtttcctgcc agtacagata atcatcccat gattcctcag
     6781 accagatta
//
LOCUS       DAJTUP010000165         6757 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTUP010000165 DAJTUP010000000
VERSION     DAJTUP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.167"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1210
                     /locus_tag="OO468_003772"
     CDS             <1..1210
                     /locus_tag="OO468_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR6260713.1"
                     /translation="GIPLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRD
                     YHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTD
                     EAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSV
                     DQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPM
                     FMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPH
                     KNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSL
                     VYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFN
                     NIKKALSEAK"
     gene            1262..2419
                     /locus_tag="OO468_003773"
     CDS             1262..2419
                     /locus_tag="OO468_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR6260714.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2385..2507)
                     /locus_tag="OO468_003774"
     CDS             complement(2385..2507)
                     /locus_tag="OO468_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260715.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2710..4395
                     /locus_tag="OO468_003775"
                     /pseudo
     CDS             2710..4395
                     /locus_tag="OO468_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4433..>6757
                     /locus_tag="OO468_003776"
     CDS             4433..>6757
                     /locus_tag="OO468_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR6260716.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASG"
BASE COUNT         1995 a         1473 c         1431 g         1858 t
ORIGIN      
        1 tggtattccg ctaacagaaa ctttcttgcc tgaattattc cagaatcatg gttattacac
       61 tgcagcagta ggtaaatggc acttgtcaaa aatcagtaat gtgccggtac cggaagataa
      121 acaaacgcgt gactatcatg acaacttcac cacattttct gcggaagaat ggcaacctca
      181 aaaccgtggc tttgattact ttatgggatt ccacgctgca ggaacggcat attacaactc
      241 cccttcactg ttcaaaaatc gtgaacgtgt ccccgcaaaa ggttatatca gcgatcagtt
      301 aaccgatgag gcaattggcg ttgttgatcg tgccaaaaca cttgaccagc cttttatgct
      361 ttacctggct tataatgctc cgcacctacc aaatgataat cctgcaccgg atcaatatca
      421 gaagcaattt aataccggta gtcaaacagc agataactac tacgcttccg tttattctgt
      481 tgatcagggt gtaaaacgca ttctcgaaca actgaagaaa aacggacagt atgacaatac
      541 aattattctc tttacctccg ataatggtgc ggttatcgat ggtcctctgc cgctgaacgg
      601 ggcgcaaaaa ggctataaga gtcagaccta tcctggcggt actcacaccc caatgtttat
      661 gtggtggaaa ggaaaacttc aacccggtaa ttatgacaag ctgatttccg caatggattt
      721 ctacccaaca gctcttgatg cagccgatat cagcattcca aaagacctta agctggatgg
      781 tgttcccttg ctgccctggt tgcaagataa gaaacaaggc gagccacata aaaatctgac
      841 ctggataacc tcttattctc actggtttga tgaggaaaat attccattct gggataatta
      901 ccacaaattt gttcgccatc agtcagacga ttacccgcat aaccccaaca ctgaggactt
      961 aagccaattc tcttatacgg tgagaaataa cgattattcg cttgtctata cagtagaaaa
     1021 caatcagtta ggtctctaca aactgacgga tctacagcaa aaagataacc ttgccgccgc
     1081 caatccgcag gtcgttaaag agatgcaagg cgtggtaaga gagtttatcg acagcagcca
     1141 gccaccgctt agcgaggtaa atcaggagaa gtttaacaat atcaagaaag cactaagcga
     1201 agcgaaataa ctaaaccttc atgcggcgga tttttccgcc gccttattga gcgagatagc
     1261 gatgcacgtt acagccaagc cctccagttt tcaatgtaat ctcaaatgtg attactgttt
     1321 ttaccttgaa aaagagtcgc agtttactca tgaaaaatgg atggatgaca gcactctgaa
     1381 agagttcatc aaacaatata tcgaagcgtc tggcaatcag gtctatttta cctggcaagg
     1441 cggtgaaccc actctggctg gcctggattt tttccgtaaa gttattcact atcaacaacg
     1501 ctatgcaggc caaaaacgta tttttaatac attacaaacg aatggcattt tattgaataa
     1561 tgaatggtgt gcctttctca aagaacatga atttctggtt ggtatctcga tcgatggccc
     1621 ccaggagtta catgaccgtt acagacgcag taattcaggt aacggtactt ttgcaaaagt
     1681 gatagcagcc atcgagcgtc tgaaatcata tcaagtagag tttaatacgt taaccgtcat
     1741 taataacgtt aatgtccatt atcctcttga ggtttatcat tttttaaaat ctatcggcag
     1801 taaacatatg caatttatcg aattgctaga aaccgggacg ccgaatattg atttcagtgg
     1861 tcatagtgag aacacattcc gtatcattga tttttctgtg cctcccacgg cttatggcaa
     1921 gtttatgtca accattttta tgcaatgggt taaaaacgat gtgggtgaaa ttttcattcg
     1981 tcagtttgaa agctttgtca gccgtttttt ggggaatggg catacaagtt gtattttcca
     2041 ggagtcctgc aaggataatc tggttgttga aagtaatgga gacatttacg aatgcgacca
     2101 ttttgtctat ccacagtaca aaattggaaa cattaataaa tctgaactca aaacgatgaa
     2161 cagtgtacaa ctgacagcgc aaaaaaaacg gatttcagcg aaatgtcagc aatgtgcata
     2221 taaacctatc tgcaatggcg gttgtcctaa gcatcgtatt actaaagtaa acaatgagac
     2281 tgtttcttat ttttgcgaag gttataaaat ccttttttca accatggtac cttatatgaa
     2341 cgccatggtt gagttagcta agaacagagt accgctttac cacattatgg atgttgcaaa
     2401 acaaatggag aataattaat aacgctctct tttaactcca ttcttagcct ccttcttatg
     2461 accttaacaa ggcacccgac ataaattcct gtcgggtgtt tcgtcatcat catcttccct
     2521 tgcgaaaatc ctggcttacg tggtatgcgt cagggcatta gcaataccct tgattatcaa
     2581 tagcataacc ataaccactg aaaattaaaa atttctatat atttcatatt gttaaatgat
     2641 gcttaaataa atttcatcag taaatcattg ccatagataa tcattggatg gcacaatcaa
     2701 ttttcgctaa tgataaccat tcccattacg ttttgtatgt tgatagcaaa gtatctctgt
     2761 ctgctgaagc ccttttggct gcgtaagaat aataaaactt cagtgttgtt aatcattatt
     2821 atcctcgcaa tgatccttgg cgtggttaaa attcaggtct ggttaaacga ctggaataat
     2881 gattttttca atgcgctaag ccagaaagaa accgataagc tttggcagct cgttctttgg
     2941 ttccctgccc tgctggggat ctttgtatta atatccgcaa acaaaacatg gttaattaaa
     3001 ctactgacta ttcgctggcg cgaatggtta acagattact accttaatcg ctggtttgca
     3061 gataaaaact actacttcac gcagatctac ggcgagcata aaaatacaga taaccctgac
     3121 caacgtatcg ctgaagacat tctcttactg atcaacaaaa cgctcagtct ttcatttggc
     3181 ttcatccagt cacttagcat gctgatcacc tttaccgtta ttctttggca aagcgcgggt
     3241 acgctctcat tcactgttgg tggaacagaa tggaatatcc aggggtatat ggtctatacc
     3301 gtcgtgctca ttgtgatcgg tggaacttta tttacccata aggtgggtaa acgtattcgt
     3361 ccgcttaatg tggaaaaaca acgtagcgaa gcgacatttc gaactaatct tgtgcagcat
     3421 aataagcagg cagaactgat tgcgctaagc aatgctgagt ctctacaacg ccaggagttg
     3481 cgcgacaatt ttcataccat taaagagaac tggcatcgtt taatgaatcg tcagcggtgg
     3541 cttgattact ggcaaaatat ttattcgcgc tcgcttagcg ttcttcccta ctttctgtta
     3601 ttgccgcagt ttattagcgg gcagatcaat ctgggcggac tgatgaaatc gcgccaggca
     3661 tttatgctag tatcgaacaa tttaagctgg tttatttata aatatgacga acttgctgaa
     3721 ctggctgcgg ttatcgatcg cttgtatgag ttccatcaac tcactgaata gcgccctacg
     3781 aataagccta aaaattgcca acatgcggta caagtggcta atgcgagtat tcgtacgcct
     3841 gataataaga tcatattaga gaacttgaac tttcatgttt cgccaggcaa atggctatta
     3901 ctgaaaggct actctggtgc gggaaaaacc acactgctta aaacattatc ccactgctgg
     3961 ccgtggttta aaggtgatat ttcttctcct gctgacagtt ggtatgtgtc acaaacaccg
     4021 ttaatcaaaa gcggcttact gaaagagatt atttgtaaag cacttcccct gcccgtagac
     4081 gataaatcgt tgagcgaagt actgcatcag gttggtctgg gaaaattggc tgcgcgtatt
     4141 catgaccacg atcgctgggg agatattctt tccagcggcg aaaaacaacg tatcgccctg
     4201 gcacgattaa ttttacgacg tccgaaatgg atatttcttg acgaaactac ctctcatctt
     4261 gaggaacaag aggctatacg cttactgcgt ttagtgcgtg aaaaactacc cacaagcggc
     4321 gtcattatgg ttacacatca acccggtgtc tggaacctgg ctgatgatat ttgtgacatt
     4381 agcacggttt tataacaaca ataaaagccc tgctgtacgg agatataaat aaatgaagcg
     4441 agttcttatt cctggcgtca ttttatgtgg cgctgatgtg gcgcaggccg tcgatgacaa
     4501 aaacatgtac atgtattttt ttgaagagat gacggtctat gctcctgtcc ctgtacccgt
     4561 aaacggcaac acgcattaca ccagtgaaag catcgagcgt ttaccgaccg ggaatggcaa
     4621 tatcagcgat ctgctgagaa ccaaccctgc ggtacgcatg gattcaacgc aaagtacctc
     4681 gttgaaccag ggagatattc gccctgagaa aatctctatt cacggtgcgt cgccctacca
     4741 gaatgcctat ttgattgacg gtattagtgc aactaataac ctgaacccag cgaatgagtc
     4801 cgatgccagt agtgcaacca atattagcgg gatgtcacag gggtattatc ttgatgtcag
     4861 cttactggac aatgtgacgc tttatgacag ttttgtgccg gttgaatttg gtcgcttcaa
     4921 tggcggggta attgatgcaa agatcaaacg cttcaacgct gatgatagca aggtgaaatt
     4981 gggttatcgc actacgcgtt tggactggtt aacatcgcat atcgatgaga ataacaagag
     5041 cgcatttaat caaggttctt caggaagtac ctatttctct ccagatttta aaaagaactt
     5101 ttataccttg tcgtttaatc aggagctggc tgataacttt ggcgttaccg ccggtttatc
     5161 gcgccgccag tctgatatca cccgcgcgga ttatgtttcg aatgacggca ttgtcgccgg
     5221 tcgggcacag tataaaaacg ttatcgatac tgcattgagc aaatttacct ggtttgccag
     5281 cgaccgcttt acccacgatt taaccttaaa atataccggc tccagccgtg attataatac
     5341 cagcaccttc ccgcagtctg atcgcgaaat gggtaataaa tcctatggtc tggcatggga
     5401 tatggatacg caactcgcat gggccaaact acgtaccacc gttggttggg atcatattag
     5461 tgattatacc cgtcacgatc atgacatctg gtacaccgaa ctttcatgta catatggtga
     5521 tattacaggg cgttgtaccc gtggcggatt aggacacatt tcccaggctg tagataatta
     5581 caccttcaaa acacgcctgg actggcaaaa attcgccgtg ggtgatgttt cgcatcaacc
     5641 ctacttcggc gcggaataca tctattccga tgcatggact gaacgccata accagtctga
     5701 atcctatgtg attaatgctg caggaaagaa aactaaccat accatttacc ataaaggtaa
     5761 aggcagcctg ggaattgaca actacacgct gtatatggcg gatcacatta gctggcggaa
     5821 tgtgtcgtta atgcccggtg tgcgttatga ctatgacaac tatctgtcaa accacaatat
     5881 ctccccgcgc tttatgacgg aatgggatat ttttgctgat caaacctcaa tgattaccgc
     5941 cggttataac cgttactatg gcgggaatat tcttgatatg ggattacgtg atatccgcaa
     6001 tagctggacg gaatcggtat caggtaataa aaccctgacg cgttatcaga atttgaaaac
     6061 gccttataac gatgaactgg caatgggatt gcagcaaaaa atcgataaga acgttattgc
     6121 acgcgcaagt gaagcgcatg atcaaatcag caaaagcagt cgtaccgaca gcgcgactaa
     6181 aaccaccatt actgaatata acaacgatgg caaaaccaaa acgcattcgt ttaacctcag
     6241 ttttgaactg gccgaacccc tgcatatccg ccaggtagat attaacccgc aaattgtctt
     6301 tagctatatc aagagcaagg gcaacttgtc gttaaacaat ggttatgagg agagcaatac
     6361 cggtgataac caggtggttt ataacggtaa tctggtctct tacgatagcg ttccagtggc
     6421 agattttaat aacccattaa agatctcctt aaacatggat ttcacgcatc aaccgagcgg
     6481 gttagtgtgg gcgaatacgc tggcctggca agaagcgcgt aaagctcgca ttatccttgg
     6541 taagacaaat gcgcaataca tcagcgaata ttcagattac aagcagtatg ttgacgaaaa
     6601 actggatagc agcctgacct gggacacccg cttgtcctgg acgccacaat ttctgaaaca
     6661 acaaaacctg acgatcagtg ccgatattct caatgtactg gatagcaaaa ccgctgttga
     6721 tacaacgaac accggtgtgg cgacctacgc cagtggc
//
LOCUS       DAJTUP010000166         6596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTUP010000166 DAJTUP010000000
VERSION     DAJTUP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.168"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..992
                     /gene="ydfJ"
                     /locus_tag="OO468_003777"
     CDS             <1..992
                     /gene="ydfJ"
                     /locus_tag="OO468_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR6260717.1"
                     /translation="IQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSA
                     TAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPT
                     AKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMIS
                     SILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIV
                     IHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTES
                     WYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            1081..2541
                     /locus_tag="OO468_003778"
     CDS             1081..2541
                     /locus_tag="OO468_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR6260718.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2577..2780)
                     /gene="ydfZ"
                     /locus_tag="OO468_003779"
     CDS             complement(2577..2780)
                     /gene="ydfZ"
                     /locus_tag="OO468_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR6260719.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2958..3644)
                     /gene="ydfH"
                     /locus_tag="OO468_003780"
     CDS             complement(2958..3644)
                     /gene="ydfH"
                     /locus_tag="OO468_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR6260720.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3733..4479)
                     /gene="ydfG"
                     /locus_tag="OO468_003781"
     CDS             complement(3733..4479)
                     /gene="ydfG"
                     /locus_tag="OO468_003781"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR6260721.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4616..>6596
                     /gene="dcp"
                     /locus_tag="OO468_003782"
     CDS             4616..>6596
                     /gene="dcp"
                     /locus_tag="OO468_003782"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR6260722.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLH"
BASE COUNT         1634 a         1547 c         1707 g         1708 t
ORIGIN      
        1 ttattcaggg attgggtgca ggtgcggaaa tttccggtgc cggtacgatg ctggcggaat
       61 atgcgccaaa aggtaagcgc ggaattatct cctcatttgt ggctatggga actaactgcg
      121 gcaccttaag cgcaacggca atctgggcct ttatgttctt cattctcagt aaagaggaac
      181 tgctggcgtg gggatggcgt ataccgttcc tggcgagcgt tgtcgtgatg gtctttgcta
      241 tctggttgcg tatgaatctg aaagaaagcc cggtctttga gaaggttaac gacagcaacc
      301 aaccgacagc aaaacctgca cctgctggta gcatgttcca gagcaaatcc ttctggctgg
      361 caacagggct gcgttttggt caggcgggta actccggttt aattcagact ttccttgcag
      421 gctatttagt acagacgtta ttgtttaaca aagcaattcc aacagatgca ttgatgatca
      481 gttcgattct cggctttatg accattccgt tccttggttg gttatccgat aaaattggtc
      541 gccggatccc gtatattatt atgaatacct ctgcgattgt gctggcgtgg ccaatgcttt
      601 ctatcatcgt agataaaagc tatgccccga gcaccattat ggttgcactg attgtgattc
      661 ataactgtgc ggtgctggga ttatttgctc tggaaaacat taccatggca gaaatgttcg
      721 gctgtaaaaa ccgcttcacc cggatggcta tttcaaaaga aattggtggt cttatcgctt
      781 ccggttttgg tcctatcctg gcgggtattt tctgcaccat gacggaatcc tggtatccga
      841 tcgccattat gatcatggca tattcagtga ttggtttaat ctctgcgctg aaaatgccag
      901 aggtgaaaga ccgtgattta agtgcgctgg aagacgctgc ggaagatcaa ccgcgtgttg
      961 taagagctgc gcaaccttcc agaagtctgt aaaccctcaa tcccttctct taccggagag
     1021 gggattttta ttcatataac aaaacatata gcttgccata tttaaattta aggaattgtt
     1081 atgggaaata atttgttatc agcaaaagcg acactccctg tttatgatcg taataacctg
     1141 gccccaagaa ttgttcattt aggctttggt gcatttcacc gtgcgcatca gggcgtgtat
     1201 gccgatattc ttgctacaga acatttcagt gactggggat attatgaggt caacttaatc
     1261 ggcggcgaac agcaaattgc cgatttacaa cagcaagata atctttatac cgttgcggaa
     1321 atgtcggccg atgcgtggac ggctcgcgtc gttggcgtcg ttaaaaaagc cttgcacgta
     1381 cagatggatg gcttagaaac cgtgttggct gcgatgtgtg aaccgcaaat cgcgattgtc
     1441 tctctgacaa tcaccgaaaa agggtatttc cactctccgg cgaccggaca gttaatgctc
     1501 gagcacccga tggtcgctgc cgacgtgcaa aatccccacc agccgaaaac agcaacaggg
     1561 gtgattgttg aggcgctggc tcgccgtaaa gcggcaggac ttcccgcatt taccgtcatg
     1621 tcatgtgaca acatgccaga aaacggtcat gttatgcgtg acgttgtcac ttcctacgcg
     1681 caagccgttg atgtaaaact ggcacaatgg atcgaagata acgtgacttt cccatcaaca
     1741 atggtggacc gtattgtgcc cgcagtgaca gaggatacgc tggcgaaaat cgaacaactt
     1801 accggtgtgc gcgatcctgc tggcgttgcc tgtgaacctt tccgccagtg ggttatcgaa
     1861 gataactttg tagccggacg tccggaatgg gaaaaagcgg gagccgaact ggttagtgat
     1921 gtgctgcctt atgaagagat gaagttgcgc atgctcaacg gcagtcattc attcctggca
     1981 tatctgggtt atcttgctgg atatcagcac attaatgact gtatggaaga tgaacattat
     2041 cgtcatgcgg cgtatggcat gatgttgcag gaacaagcgc cgacgctgaa agtgcagggc
     2101 gttgatttgc aagattacgc taaccgatta attgcacgct atagcaaccc ggcgttacgt
     2161 catcgaacct ggcagattgc gatggatggc agccagaaat tgccacagcg gatgttggat
     2221 tctgttcgct ggcatctggc gcatgacagc aagttcgatc tgctggcgct gggcgtcgcg
     2281 ggttggatgc gttatgtcgg tggtgttgat gaacagggaa atccgataga aatcagtgac
     2341 ccactgttac ctgttattca gaaggctgta caaagtagtg ccgaagggaa agcgcgcgtc
     2401 cagtcattgc tggcgattaa ggcaatcttt ggtgatgatt tgccagacaa tagcttgttt
     2461 actgcaaaag tgacggaagc gtacttgtct ttattagcgc atggcgcgaa agcgaccgtg
     2521 gcgaaatatt ccgtgaagta aaaaaagcaa tgccgccatc ggcggcattc acacacttag
     2581 ttcatgccga gacgaatcag gtccagaggt gccagtttct cttcacaacc ttcaacaact
     2641 acggtttttc cgcggcggat ttgctcagcg gtcagacctt cagtatcaat cgacaggatc
     2701 ttgccagtgt gaccggtgcc gctaaccata acacggctgc cagtggtgat tgcgttacgg
     2761 ttacgatcgt aggtcgtcat aggtgttttc tcctttctga tttacagtcg aagcccacgt
     2821 aacgggcgca atataaatac tcctgtcgag tgaaatattt tttgtttttg atcaagctca
     2881 catctttttt gatgcatgca tgagccgtaa ggcgggggga aaagcctctc atcccggatg
     2941 ggatgagagt aaatgaatta ctcttcgcta aaccagtcgc tgttttcctg gcggatctgg
     3001 cgcacggatt cgctgatttc ctgcaaatgt tgcgtcattg cacgttctac cgcatcgcca
     3061 tcacgttttt gcagggcaga gaaaatatca agatgctggc gtaacagcat ttctggtgga
     3121 gaaacatggt cgaaactcat atagcgcacg cgatcaacgg tcgctttcag gttctcaatg
     3181 gtatcccacg ccagttgaca gtcggcaatc tgcgtcagga gttgatggaa gttgtcatca
     3241 agttcaaaaa aatcatccag ttgcttgcgc tcaatggcaa tgcgttgctg gtgaagattt
     3301 tgttccagtt ggtagcactg gctttcggta atcatgctcg ccgcccgacg cgccaccgcg
     3361 cactcaatgg cctgacggat aaaactgccg ttgcgcacct gggccatgga aattttgttg
     3421 acgtagctgc cacgctgcgg acgaatttga atcaggccgt tttccgccag tttaataaag
     3481 gcttcacgaa ccggctggcg tgacacattg aaacgaacag aaacttcttt ttccgacaac
     3541 ggtgtgcctg gtgcaatcag gcaatggaca atgtcgcgac gaagaatacg ataaatctgc
     3601 tgattgacag gctgtgtggg attaagttgc gtttcgacgg tcattcgttc ttgcttgtga
     3661 gtgagttaac tgcatgagtc taccactttt ttgtgacaag caataacccg gcagttacgc
     3721 cgggtataaa aattactgac ggtggacatt cagtccggca tagctttggg taaccggcat
     3781 catttccagg gtattgatat tgacgtgagc aggcagcgtt gacacccacc agacggcttc
     3841 gctgacatct tctggcgtca atgcaacggt attttgatag gttttttccg ctttaccgtc
     3901 atcgccttta aagcggacat tggaaaactc ggtgccaccc accagacccg gttcgatgtc
     3961 ggtgacgcgc accgccgtac catgcagatc cgtacgcaga ttcaggctaa actgacgaac
     4021 aaacgctttc gtcgcaccgt aaacgttacc accggcatac ggccagctgc ctgccgttga
     4081 gccaatgtta ataatatgac catgattacg ttcaaccata cccggtaaga cggcgcgcgt
     4141 catatatact aggcctttgt tgttggtatc aatcatcgtt tcccagtctt caacgctggc
     4201 tttatgcgca ggctccatgc ccaacgccag gccggcatta tttaccagga tatcaatatt
     4261 gctccactcg gcagggagcg atgccagcat ctcttcaata gcggcgcggt tgcgaacgtc
     4321 cagttgggcg atatacagat tatctcccag ttcgtctgtt aactcctgca accgctcctg
     4381 gcgacggcca gtggcgataa ctttatgccc ttgttgaata aaacgacgag taatgcattc
     4441 accaaaacct gccgttgctc cagttactaa aacgatcatc tcactgttcc tcaacgcttt
     4501 tgtgtgtcaa taacatagca cgtgatgctt aagagcgggt aatactggtt tgcaagattg
     4561 cactccgtta tcagtaagcc tacgctatgg aacacctcgc gttcaggaga agaaaatgac
     4621 aacaatgaat cctttccttg tgcaaagcac actgccgtat ctggctcctc attttgatca
     4681 aattgccaat catcactatc gcccggcatt cgatgaggga atgcaacaaa agcgggcaga
     4741 aattgctgcc atcgcgctta acccgcaaac gcctgatttc aacaatacta ttctggcact
     4801 ggaacaaagc ggagaattaa ttacccgcgt taccagcgtc ttttttgcga tgactgcggc
     4861 gcataccaat gatgaattac agcgtcttga tgaacagttt tccgctgaac tggcggaact
     4921 ggctaatgat atctatctga acggtgaatt attcgcgcgg gtagatgctg tctggcagcg
     4981 ccgtgaatcc ctggggcttg atagtgaatc catccgcctg gtggaggtta ttcatcaacg
     5041 ttttgtcctt gccggagcca aacttgcaca agctgataaa gcaaaattaa aagtactgaa
     5101 tacagaagct gcgaccctga ccagccagtt taaccagcgg ttacttgcag caaataaatc
     5161 cggcggtctg gttgtgaacg atatcgcgca gctggcagga atgagtgagc aagagattgc
     5221 gctggcggca gaggcggctc gcgagaaagg tctggataac aaatggctga ttccgctgct
     5281 gaataccacc caacaaccgg cgcttgccga aatgcgcgat cgtgcgacgc gtgaaaaact
     5341 gtttattgcg ggctggacgc gagcggaaaa aaatgatgcc aatgataccc gcgctatcat
     5401 tcaacgtctg gtagagattc gcgcacagca ggcgaaactg cttggttttc ctcattatgc
     5461 cgcatggaaa atcgccgatc agatggcaaa aacgccagaa gcagcactca actttatgcg
     5521 ggaaattgtt ccagcggcgc gtcaacgtgc gagcgatgaa ttagcctcca tacaggcggt
     5581 tatcgataag cagcagggcg ggtttagcgc gcagccgtgg gactgggcat tttatgcgga
     5641 acaggtacgg cgggagaaat ttgatcttga tgaggcgcag ctcaagccat attttgaatt
     5701 aaacacggtg ttaaatgaag gtgtattctg gaccgcgaat cagctcttcg gtattaagtt
     5761 tgtcgaacgt tttgatattc cggtctacca tcctgacgtt cgtgtgtggg aaatttttga
     5821 tcataatggc gtgggactgg cgttatttta cggtgatttc ttcgcccgtg attcaaaaag
     5881 cggcggtgca tggatgggca attttgttga gcaatcaacg cttaatgaaa cgcatccggt
     5941 aatttataac gtctgcaatt atcagaaacc cgctgccggt gagcctgcgt tgttactctg
     6001 ggatgatgta ataaccttat tccatgaatt tggtcatacg ctgcacggcc tttttgcccg
     6061 ccagcgttat gccacgcttt ccggcaccaa cacgccgcgt gattttgtcg aatttccgtc
     6121 gcaaatcaac gaacactggg caacgcatcc gcaggtattc gctcgctacg cccggcatta
     6181 tcagagcggg gcagcaatgc ctgacgaact gcaacagaaa atgcgtaatg ccagcctgtt
     6241 caacaaaggg tatgagatga gcgaactgct tagcgccgca cttctcgata tgcgctggca
     6301 ttgcctggaa gaaaacgaag caatgcagga tgtcgatgat tttgaattgc gggcgctggt
     6361 ggcggaaaat atggatcttc ctgctatacc gccacgctat cgcagcagtt atttcgccca
     6421 tatttttggt ggcggatatg ctgcaggtta ttacgcttat ctgtggacgc aaatgttggc
     6481 cgatgatggt taccagtggt ttgttgagca gggcggatta acgcgtgaaa atgggcagcg
     6541 ttttcgcgag gcgatccttt ccagaggtaa cagcgaagat ctggaacgcc tgcatc
//
LOCUS       DAJTUP010000167         6587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTUP010000167 DAJTUP010000000
VERSION     DAJTUP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.169"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..600)
                     /locus_tag="OO468_003783"
     CDS             complement(<1..600)
                     /locus_tag="OO468_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310145.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6260723.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTT"
     gene            complement(679..1632)
                     /gene="lsrR"
                     /locus_tag="OO468_003784"
     CDS             complement(679..1632)
                     /gene="lsrR"
                     /locus_tag="OO468_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR6260724.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            1881..3416
                     /gene="lsrA"
                     /locus_tag="OO468_003785"
     CDS             1881..3416
                     /gene="lsrA"
                     /locus_tag="OO468_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR6260725.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            3410..4438
                     /gene="lsrC"
                     /locus_tag="OO468_003786"
     CDS             3410..4438
                     /gene="lsrC"
                     /locus_tag="OO468_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR6260726.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            4438..5430
                     /gene="lsrD"
                     /locus_tag="OO468_003787"
     CDS             4438..5430
                     /gene="lsrD"
                     /locus_tag="OO468_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR6260727.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            5442..6465
                     /gene="lsrB"
                     /locus_tag="OO468_003788"
                     /pseudo
     CDS             5442..6465
                     /gene="lsrB"
                     /locus_tag="OO468_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            6492..>6587
                     /locus_tag="OO468_003789"
     CDS             6492..>6587
                     /locus_tag="OO468_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR6260728.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGAL"
BASE COUNT         1512 a         1648 c         1735 g         1692 t
ORIGIN      
        1 ggtggttaga tcaagaagtc ccgtggtgcc agcgttagag ggatccaccg ccagttcgcc
       61 gctgagcata taggccagcc agtcgctgat catggtgatg gttgatgcct gacggtaaat
      121 atcggaacga tggtgcgcca gccaaagtaa tctggggatg gcacttaaag ccagtgtttg
      181 tccggtcgct cgataaactt cgttttcaaa ggtattgttg tgcagttctt taagttcgct
      241 aacttcgcgt gccgctctgg catccacatt ggcgcaggcc cagatcggtg ttccttcatt
      301 gttatataaa acaatgcctt cacgcataga acatgccgaa acggcagcga tatactccgg
      361 gtctatgccg gcgttgtgca gcgcctggcg catacactca cacgccagtt gccagttttt
      421 gttgagatca aattccatag aaccaggaac gtccggtact gccagatgcc gccactccgc
      481 ctgtcccact gctatttgat tgccttccag gtcgaatatc acagcccgaa tacttccggt
      541 gcctgcatcc agcgccatca ggtagtactt tgattctgaa ggggtaaaga gtcgagccat
      601 tagattatcc tcggcttatg cgttctgtct gttcctctat acgttctcca tcattcccgg
      661 taataaggtc atgcaaattt aactacgtaa aatcgccgct gctgtgtcct gatcggtaac
      721 cagtgcgttg atataaccgc ctttcattgc agcggcaatt gcttcagctt tattttctcc
      781 ccctgccacg ccaacccgga cgggtatggt cttcagcgcg cttaaaggta agccaatcag
      841 ttcgttatgt attttgatat ccgtgacaac gtcacctttt gcatcaaaaa agtagcctaa
      901 aatgtcgcca accgcccctt ttcggccaat cattaactgt tcgccctggc tgatataacc
      961 ggagcgaatg attgtcgcgt cgtcttgttg actcacggct ccaatgccga caatcgccac
     1021 atccgctgct tgcgcggcta gcagaacatc tttgacgcaa ttttcatttt ttagcgtacg
     1081 ggcaatgtca gcggaggatg cccgcaacgg agccggaata atattcacac tgcacgccgc
     1141 gttaagctgc ccgatacccg tcatataaga accgacgcca ccggagagcg tgaccaggcg
     1201 aatttgctgt gacgaaataa aaccacttaa gcgttgcagc gtattcatgg ttgcctcgcc
     1261 aaaaccaatc gccagcatct gttgtggttg aagtaaactc atcaacatat gcgccgcgcc
     1321 tatccctagt cgcccgccga catcagcatc cgcaagccca gggatcaccc ggacatgttg
     1381 cagcgaaaac tgacgacgta attgagtttc atattccaga cagccttcaa agcgagaatt
     1441 aatctgtacg cgaataatgc cggactgatg ccctttctcc agcaagcgcg acactttcaa
     1501 acgggtcagg ccgagacgat cgctgatctc gctctgggtc agcccgtcgt gatagtaaaa
     1561 ccacgcgatc cgcgcgacct gttcttcttc acacattccc tgttctgaaa ttgccgaatc
     1621 gttgattgtc ataattcatt cttcactttg aacatattta aatctttaat gcaattgttc
     1681 agttcttgcg catttatatc tgtgatggca accacagttt gactctacga gcatgaacaa
     1741 acgcaaccgt gaaaatcaaa atagcataaa ttgtgatcta ttcgtcggaa atatgtgcaa
     1801 tgtccaccta aggttatgaa caaattaaaa gtagaaatac atttgttcaa aactcacctg
     1861 caaaactgaa cgggggaaat atgcaaacga gtgatacccg cgcgttaccg ctactttgcg
     1921 cccgctcggt ttataaacag tattcagggg tcaatgtcct gaaaggcatc gattttacgt
     1981 tgcatcaggg ggaggtccac gccctgctcg gcggcaatgg tgccggtaaa tcgacgttaa
     2041 tgaagattat tgccggtatt acccctgctg atagcggtac gctggagatt gggggcaaca
     2101 actacgccag attaacgcca gttcatgctc atcagctggg catttatctc gttccccagg
     2161 aaccgctgct tttcccaagc ctgtcgataa aagaaaacat cctgtttggg ctggcaaaaa
     2221 aacagctctc catgcagaaa atgaagaact tgctggcggc gctgggctgc cagtttgatc
     2281 tgcatagtct ggcaggatcg ctggatgtcg ccgatcgcca aatggtggaa atcctccgcg
     2341 ggctgatgcg cgactcgcgg attctgatcc tcgatgaacc taccgcctcg cttacccctg
     2401 cggaaaccga acgcttgttt agtcgcttgc aagagctgct tgctactggc gtgggtattg
     2461 tttttatctc gcataagctg ccggaaattc gccagattgc cgatcgaatt agcgtgatgc
     2521 gcgacggaac catcgcctta agcggcaaaa ccagcgaact gtctaccgac gacattattc
     2581 aggccatcac cccagcggta cgggaaaaat cgctctctgc cagccaaaaa ttatggctgg
     2641 agttacctgg taaccgccca caacatgccg tcggaacgcc ggtgctgaca ctggaaaatc
     2701 tgactggcga aggcttcagg aatgtcagcc tgacgctcaa tgccggagaa attctgggcc
     2761 tggctgggct ggtgggagcc ggacgcacag aactggccga gacgctctat ggtctgcgta
     2821 ctttgcgtgg cggacgcatt atgctgaatg gtaaagagat caataaatta tccactggag
     2881 aacgtttact gcgcggtctg gtttatctgc cggaagatcg ccagtcatcc ggactgaatc
     2941 tcgatgcttc gctggcctgg aacgtctgcg cccttactca taaccttcgt ggattctggg
     3001 cgaaaaccgc gaaagataat gccaccctgg aacgttatcg tcgggcgctg aatattaaat
     3061 ttaaccaacc ggaacaagct gcacggacat tatccggtag caaccagcaa aaaatcctca
     3121 ttgccaaatg cctggaagct tcgccgcaag cattgattgt cgatgagccg acgcgcggcg
     3181 tggatgtctc ggcccgtaat gatatctacc agctgttgcg cagcatcgcc gcccagaatg
     3241 tggctgtgct gtttatctcc tctgatatgg aagagatcga actgatggca gatcgtgtgt
     3301 atgtgatgca tcagggcgaa attacccact ctgcactgac cgggcgcgat attaatgtcg
     3361 agactattat gcgcgttgcc ttcggcgata gtcagcgtca ggaggcatca tgctgaagtt
     3421 tattcagaac aaccgtgaaa tcacggcatt gctggcggtg gtgctgctgt ttgtattacc
     3481 cggttttctc gaccgccagt atttaagtgt gcaaacgctg accatggttt atagcagcgc
     3541 gcaaatcctg atcctgctgg caatgggcgc gacgctggta atgcttacgc gcaatattga
     3601 tgtttcagtg ggttcgatta ccggaatgtg cgcggtgctg ttggggatgt tactgaacgc
     3661 aggatattca ctacctgttg cctgtgtcgc gactttactg cttggtttgc tcgcgggatt
     3721 tttcaacggt gccctggtcg cgtggctaaa gatccctgcc attgttgcca cccttggcac
     3781 gttagggttg tacagaggca tcatgttgct gtggactggc ggtaaatgga ttgaagggtt
     3841 acccgccgaa ctgaaacagc tctccgcccc gctgctgctt ggcatttcag caattggttg
     3901 gttgacgata attctggtgg catttatggc ctggctgctg gcgaagacgg cgtttggacg
     3961 cagtttttat gccacgggcg ataatttaca gggcgctcgt caactgggcg ttcgtacgga
     4021 agccattcgc attgtggcat tttcgttgaa cggctgcatg gcggcactgg cgggaattgt
     4081 gtttgcttcg cagattggtt ttatcctcaa ccagaccggt accgggctgg agatgaaagc
     4141 aattgcagcc tgcgtgctgg gcggcattag tttgctcggt ggttccggtg cgatcatcgg
     4201 tgcggtactc ggcgcatggt tcctgacgca gatcgatagc gtactggtgc tgttgcgcat
     4261 tccggcatgg tggaatgatt ttatcgcggg tctggttctg ctggcggtgc tggtgtttga
     4321 tggacgcctg cgttgtgcgc tggaacgtaa tctacggcgg caaaaatatg cccgctttat
     4381 gacgccaccg ccatccgtta aacccgcttc gtcaggtaaa aaacgggagg ccgcataatg
     4441 cgtattcgct acggttggga actggctctt gccgcactgc tcgttattga gattgtcgca
     4501 tttggtgcaa ttaacccgcg aatgttagat ctcaatatgt tgctgttcag caccagtgac
     4561 tttatctgca ttggcattgt cgctctaccg ctgacgatgg tgattgtcag tggcgggatc
     4621 gatatttcgt ttggttcgac catcggcctc tgcgccattg cattgggcgt actgtttcaa
     4681 agtggtgtgc cgatgccgct ggcgatactc ctgaccttac tgctcggcgc attgtgcggg
     4741 ctgatcaacg ccggattaat tatctatacc aaagttaacc cgctggtgat tacgcttggc
     4801 acgctgtatc tgtttgccgg aagcgctctg ctgctttccg gtatggccgg agcgacgggg
     4861 tacgaaggta ttggtggatt cccgatggcg tttacagatt tcgctaacct ggatgtgctg
     4921 ggactccccg ttccgctgat tatcttcctg atatgtctcc tcgttttctg gctctggctg
     4981 cataaaaccc atgccggacg taatgtgttt ttgattgggc aaagcccgcg cgtggcgctt
     5041 tatagcgcga ttccagttaa ccgcacctta tgtgcgctct atgccatgac ggggctggcg
     5101 tctgcggtcg ccgctgtgct gctggtatcg tattttggtt cagcacgttc cgatctcggt
     5161 gcgtcgtttc tgatgcccgc catcaccgcc gtggtgcttg gcggggccaa tatttatggt
     5221 ggttccggtt ccattatcgg caccgccatt gcggttttat tagtgggata tttgcagcaa
     5281 ggtttgcaaa tggcaggagt gccaaatcag gtgtccagcg ccctttccgg tgcgctgctt
     5341 atcgtcgttg tcgtaggtcg ttccgttagc ctgcatcgcc agcaaattaa agagtggctg
     5401 gcgcgtcggg ccaataaccc attgccataa aggatatctt catgacactt catcgcttta
     5461 agaaaatcgc cttacttagc gctcttggca ttgccgcaat ctctatgaat gtgcaggccg
     5521 cagagcgtat tgcatttatt cccaaactgg ttggcgtggg attttttacc agcggtggca
     5581 acggcgcaca acaagcgggt aaagagctga gcgttgatgt gacctacgac gggccgacag
     5641 aacccagtgt ttctggtcag gtacagttga ttaataactt cgtcaatcaa ggttataacg
     5701 ccattattcg tttctgcggt ttcgcctgat ggcttgtgtc cggcactgaa acgcgccatg
     5761 caacgtggtg tgagagtgct gacctgggac tctgatacta aaccggagtg ccgctcttac
     5821 tacattaatc agggaacgcc cgcccagttg ggaggtatgt tggtggatat ggcggcgcgt
     5881 caggtgaata aagacaaagc caaagtcgcg tttttctact caagccccac cgttacggac
     5941 caaaaccagt gggtgaaaga agcgaaagcg aaaatcgcca aagagcatcc aggctgggaa
     6001 attgtcacta cgcagtttgg ctataacgat gccactaaat cgttacaaac cgcagaagga
     6061 atattaaaag cgtatagcga tctcgacgcc attatcgccc ccgatgccaa cgccctgccc
     6121 gctgccgcac aagccgcaga aaacctgaaa aatgacaaag tagcgattgt cggattcagt
     6181 acgccaaatg tgatgcgccc gtatgtagag cgcggcacgg tgaaagaatt tggactgtgg
     6241 gatgtggttc agcaaggcaa aatttcagtg tatgtcgcgg atgtattatt gaaaaaagga
     6301 tcaatgaaaa cgggcgacaa gctggatatc cagggcgtag gtcaggttga agtctcgcca
     6361 aacagcgttc agggctatga ctacgaagcg gatggtaatg gcatcgtact gttaccggag
     6421 cgcgtgatat tcaacaaaga gaatatcggc aaatacgatt tctgatgtgc attacttaac
     6481 cggagtaagt tatggcagat ttagacgata ttaaagatgg taaagatttt cgtaccgatc
     6541 aaccgcaaca aaatatccct tttaccctga aaggttgcgg tgcgctg
//
LOCUS       DAJTUP010000168         6361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTUP010000168 DAJTUP010000000
VERSION     DAJTUP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.170"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..470
                     /locus_tag="OO468_003790"
     CDS             <1..470
                     /locus_tag="OO468_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260729.1"
                     /translation="RHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEAAKR
                     NVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPMTFS
                     RVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            442..876
                     /locus_tag="OO468_003791"
     CDS             442..876
                     /locus_tag="OO468_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6260730.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1649..2698)
                     /locus_tag="OO468_003792"
     CDS             complement(1649..2698)
                     /locus_tag="OO468_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR6260731.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3149..4339
                     /locus_tag="OO468_003793"
     CDS             3149..4339
                     /locus_tag="OO468_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR6260732.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4729..6189)
                     /gene="gtrII"
                     /locus_tag="OO468_003794"
     CDS             complement(4729..6189)
                     /gene="gtrII"
                     /locus_tag="OO468_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR6260733.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6186..>6361)
                     /locus_tag="OO468_003795"
     CDS             complement(6186..>6361)
                     /locus_tag="OO468_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR6260734.1"
                     /translation="VFGNPVRGYPSLLVSILFLGGVQLIGIGVLGEYIGRIYIEVKNR
                     PKYIIKKSHRGNP"
BASE COUNT         2004 a         1147 c         1267 g         1943 t
ORIGIN      
        1 ggcggcacga tcagttgctt accgcacttc gcgcgctgct gttaagccgc aagaatccgt
       61 ttggcgatat caaatcggat ggcacggtga aaacggctct cgaaaacctt ggtttgggag
      121 aagcagctaa aaggaatgta ggtacagggg cgaatcagat acctgatatg agcctgttcg
      181 cgtcaattaa taccgtaacg gctgctgcgc aaaaatttcc gtctggatta attttacagt
      241 gcggtcagtt gaatggtgcc ccgaatgtat cttcaacata cgggatgagg ttcccgatga
      301 cgttctcaag agtaattgct gtcgtagtta cattgaacgt tactggcgcg gctgggcagc
      361 cgactgtatc ggcgacaagt gtccagaaca ctggatttaa tattactgtg tcgcccggtt
      421 caggatacgg ttcatctgct gatgcgtatt acattgcaat gggatattaa caaaatgtca
      481 tatttttatt ctgcatcgac aaacggattt tattcgactg aatttcacgg caccaatatt
      541 cctgatgatg cagtggaaat ctcggaatca gagtggaaaa cactgattaa tgcacagagt
      601 gtaacaaaaa tgattacctg tggtgagaac ggtcatcctg tcattgttga ccgtccttct
      661 ccaacaccag aacaattagc cttaataaat gatgaaaaga aatctgcact gatagcagag
      721 gcaacgaatg taatagctcc gcttcaggat gcggttgatt taggtatggc aacagatgat
      781 gaaacgaaac tgttactggc atggaaaaaa tatcgggtgc tgcttatgcg tgttaatgta
      841 gtaaaacccg agtggcctat gcatccaaat aaatagactt gttaatctag gataattgtt
      901 ggaataatac tcgtttttca gtaggttcag gtagcctggg tataatgctc acttctacaa
      961 tgatttcaac ttattttatt taattgatat tacgaaactt aactcataca taaacaatat
     1021 gactcaatac tgtcctaaat agctgcgcag aaaagtgctc cactttcgct cagcccttgc
     1081 tgggcgaagc atcaggaagg cgttctcagc taggttgtgt tatatctggc ttgagttttg
     1141 ttgcccatac gcaacacatt acccatctgt aaacgacaag tgtggcagaa ttaggtgcag
     1201 ctatttagac tatatcgacg cagtaacgat aatagatatc gaaactgtac tagatattag
     1261 ctacttatgg ttaccggggg gagtcttttg agcactggtg ttatcaattg gcttcaaata
     1321 attcgccaat aaattatatt gcgatagctc atcaccttcc aggttgttta tagccagttt
     1381 atcagcgtca ccacctactg tttatgcttt tatttctttg attaataagt atatgatatt
     1441 ttaattcatc tggtgtattt ctttctcatg tgttggtcga ctgaatatct gttcagtgaa
     1501 ggggtaattt tatgtttgca cttcgaacat tcccttcagt tgctaggaaa gaaaaccgta
     1561 acatttgcgc attaatttta acagtataat ctgagcataa atatgcgcta tatatctata
     1621 aaaaggcgca attaattgcg cctttttatc attttatcaa aaatggccca ccgactgtcc
     1681 tcgatagcca atggccaatt ttatctgata catatacaca aatacaggta tatatgattg
     1741 cgcagatagg atttggaata gtcacgctga agcctataat tgctaaagcc atagggataa
     1801 ataatttggt gatatactcc attccacata tgttaagagt atttcttcca gctcttacaa
     1861 taaagtcatt tccacaaatt atttttgcta tgaaaatatt aaagattatc aagcctaccg
     1921 tggtgataat agtattaata gcttgaatat aactacttga tattatgtct gcattgaata
     1981 ttgagataat cttaaaaagt aaaggtttct ggttaagtag ttgatacgca ctcattatag
     2041 cggtaattga gaagacaatg aaaccaattt tagattttcc aaaaaacctg tcacgtgtta
     2101 attctaaaaa catacatcta ccgagaggca ataaccacca gtatgccata gcggagtcta
     2161 tgttccaaaa ccattgagga tctagtaatg ggttatgatt gagcactgtt tgtgacaaca
     2221 tgaacgataa cagtgatatg attaaaatca ctatattgtt cttgactatt tctctaaaga
     2281 ttgcatcaat tgctattata acaaaaaggc agtttataaa ccatattgtt cctacgaatt
     2341 ggttatttct aacaccatat attatatcat aaatgtggtt gtatatataa tcaatcgtct
     2401 cacctgagtt aattgtgttt attattaata tagaaaatgc aaaagtgaaa tatggaatca
     2461 ttaatctgta aaactttgac ttgatataat cgaaaacaga taagtcattt tttttgatag
     2521 taaaaaaacc agcagcgaaa aagaatagtg ggacgtgata actaaaaaca aaaggataca
     2581 atttgccagc ccccaaccca aggtgaccta aatatatagc aaaaataccg agaaatttta
     2641 aagcatcaac ccaatcatgt cttatttttc tgttgctgtc gattttgtta ttatacattt
     2701 aaagtaggct ctccagtttt gtgtgatttt taaacactca ttgcaaatat gaatgcctga
     2761 aagtttatga ttattaatca acgagttcaa tattaattta ttatttttca aagtcgcact
     2821 atataggcat ttacttccgc agtaccaccg atagtatcga tatcaaatca gcctgcggcc
     2881 gcgataactt tcgtaacatt ctgccagcgg ctttgttgaa taaatcaggt tcaagataat
     2941 cgtctctgca tcaggttaga gtttcaggca atacatcctc tttctggcat acctgccact
     3001 ctcgcaccat ggtcatagcc tttacgatct gcatactcac cagaccagag cccgctccct
     3061 tttaggctga ggaatcccca gaaaagaaga caggcataga gttttgtgat ctactgattg
     3121 cgctaacaaa ttcaatgaga tcacctctat gcctgcccgt aaagtatgcc agactttttt
     3181 ccgtaatgct ttagcttcca cgcatcagta tcgacaaaat gccattattg attctgctgc
     3241 tgctttagtc ggtggcgcat ctctttctct taccagcatt ggccggcatt tacccggtcc
     3301 tgctcgcgtg aaagataaaa taaaacgcgt tgactgtctg ttgggtaata aacgacttca
     3361 taatgatatc cctctgatat tcaaaaatat tacatccatg atgacaaata aactgtcgtg
     3421 gtgtgttatc gctgttgact ggagcggtta cccttttcag gaatatcatg tccttcgtgc
     3481 cagcctcctc tgcgacggtc gctctatccc cctgatgagt caggtcttcc cgtcaaaaaa
     3541 aaataacgag gcggtggaaa tcgcttttct ggatgcgctt tccggggcca tttctccccg
     3601 gactcgtgtt gttattgtta ctgatgctgg ttttcaaagt gcatggttcc gtcatatcaa
     3661 atcacaggga tgggatttta ttggtcgtat caggggggta gtgaagttca ggctggatag
     3721 tgacaaagat aagtggctgg acataaaaat gtgcaggggg agttcagagg caaaatacct
     3781 ggggacggga accctggccc ggaaaaaacg ctcgcagtgt gaaggacatt tttatcttta
     3841 taaacattca cctaaaggca gaaaaagcag gcgtgccaga gggaggccgg gcttaccaac
     3901 aacggaaaaa gaacaaaaag ccgcaggcag agaaccatgg ttaatattca gcaatacagc
     3961 tgagtttaat gccaaaaaaa tcatgaaact gtacagtcga agaatgcaga tagagcaaaa
     4021 ctttcgggat gaaaaaagtg aacgctttgg ttttggcctt cgggcgagca gaagtcggag
     4081 gggagagcgt tttctggtat taagtctact ggttacactg gccagtatcg tactctggct
     4141 acttggttat cattttgaaa ataaaggatt tcatttaaag tatcaggcta atagtctcaa
     4201 aaaacgaaga gtattgtctt ttctgacgct ggctgaaaac gtcctgagat ttaaccctga
     4261 acttctacga cgagcacaac ctgaaaagat attgtgccgg ttggccagca cttaccgaag
     4321 tatggtgctg gcttactgat gaaaattttc tggggatccc tcagcctttc ggggaggaat
     4381 gagggctctg atattctttt gccgtagttc atcgtgaaac cgtcggatgt catttggcct
     4441 gagtgaccca tgcctcccaa aagcagagtc gctagtgtta tgtaattagg cggttaaact
     4501 tcaattctca gtgttaagtt tgggtaaacg cttgtaaatg gtattgctaa ttttttaatt
     4561 gactaatatt agaaacggtt agtgtgattg tatatagata tttgagtgta aggtatttaa
     4621 aggggcattc agcccctccg tctatttgtg attgcatcca ttgatgtgtg tattattttg
     4681 agcttgaaaa agggaggcgc tctttaataa taaataaatc tatcaaagct aaatattaaa
     4741 tggaagccac ccttttttct ctgatagttt tattatcacc aaaccattta tttcagctat
     4801 cgaatctgga ttcggccagg aaggtaaact cttcacaatt ggataaatct tctctatttg
     4861 ttctttattt gctggtttgc aattacagat tccatagtaa ttcataaatg caaccatcct
     4921 aaaatagttg ccaccatccc accaaaaaaa agattttcca aaagtgtctg cgctcatgga
     4981 gagcatattt cctgagtcgt ttgaaccata tatatatata tattttccgt taagggaaat
     5041 gccttttgtt tgagatgtgt atgatatttc tttagctaaa gagatatctt tctgtcttgc
     5101 gagagtatca ttcaaaaata gatttttgga tgttgaaacg ccattaaata taataaataa
     5161 aaaaaacatg cagtttaggt attttataga gcgaaaattg cttaagcaag aaaacaaaat
     5221 tactgctact ataggcatta aaacaaacag gcgaggtggc gcccctgagc caacaacaac
     5281 gataaatata accggaagta tataaatgaa aatgattgat gaaatcaaat aaattgatct
     5341 tgtttttaat ttatatgtta gggtaaacat cagaattaat aaaggaatca gtaaccactt
     5401 gtataggttt aaaccagtgg gaggattgtt atagaagtta tatatattat ctatggctga
     5461 tttaacaagc catttaatat cgcttgcatt ttgtatataa tttgagatgt actcgttcga
     5521 ttcaagcgaa aaataatgct tgatagcttt ggttaatagc aggtaaatta aggtagataa
     5581 agctatgagg gatagagata aacatgaact ataaaaagat tcacgaatat ttttctcatt
     5641 tcgtattatc gaatttatct gcaacccaat gacgaatgca ataatatatg ttacgaatgt
     5701 ttggtagatt gccattgaaa gaatggacag tactatacca gaaaatatca caatcctatt
     5761 tttttgcgag tgaaaaataa ttgctgatat cgctgccagt agaaaagcaa ttcccacagt
     5821 atcagcttgg ttaagaaact ctaattgata ggagatctga gggaaggtaa taaatactaa
     5881 catacctatc aataattcat aagattctag ctttagcgat ctgcagatta taaatgctga
     5941 aatgatgata aaagataagg ctattaatgg tgttatataa agtgaaaaag gctcagggag
     6001 aaagtaatgt cgcaaaaaag tgtgaaacca ccgcccaagc gtgattgttt gataaaaatt
     6061 atttgtaaac tcgccatcaa tattaagtgt tccatcgtac aaccaccccc aataacaata
     6121 aattgatatt gcaaaacatg atatgaaggc aaggagattt gcatttttaa ataaattaat
     6181 tttaatcatg ggttacctcg atgagatttt tttatgatgt atttgggtct attttttact
     6241 tcaatataga ttctacctat atattctccg agaacaccaa tcccgatcag ttgcactcca
     6301 cccaagaaaa gtattgatac aagcagggag ggatacccgc gtactgggtt accaaagaca
     6361 a
//
LOCUS       DAJTUP010000169         6178 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTUP010000169 DAJTUP010000000
VERSION     DAJTUP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.171"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            525..1703
                     /gene="ltrA"
                     /locus_tag="OO468_003796"
     CDS             525..1703
                     /gene="ltrA"
                     /locus_tag="OO468_003796"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6260735.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1786..1965
                     /locus_tag="OO468_003797"
                     /pseudo
     CDS             1786..1965
                     /locus_tag="OO468_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1995..2477)
                     /locus_tag="OO468_003798"
                     /pseudo
     CDS             complement(1995..2477)
                     /locus_tag="OO468_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2733..3713)
                     /locus_tag="OO468_003799"
     CDS             complement(2733..3713)
                     /locus_tag="OO468_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR6260736.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3713..4912)
                     /locus_tag="OO468_003800"
     CDS             complement(3713..4912)
                     /locus_tag="OO468_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR6260737.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5234..5572)
                     /locus_tag="OO468_003801"
                     /pseudo
     CDS             complement(5234..5572)
                     /locus_tag="OO468_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5639..>6178)
                     /gene="istB"
                     /locus_tag="OO468_003802"
     CDS             complement(5639..>6178)
                     /gene="istB"
                     /locus_tag="OO468_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR6260738.1"
                     /translation="FATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAMGY
                     EAVRVGIKVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQEE
                     AKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIKGE
                     SYRLRQKRKAGVIAEANPE"
BASE COUNT         1676 a         1420 c         1464 g         1618 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtagaggtgg gccctccggt cagggtttac
       61 aggagcacag accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt gggttcggga agctgaacga aagtaaactg
      181 tccgatgaag catcgaaatc ttagatgatg ccaaaatggg gccgtacagc cgtcccctga
      241 taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg cagcaggatc
      301 ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc gggaaacagg
      361 ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagct aagggatggg
      421 gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga tgcaacgaaa
      481 atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc gtcgcaacaa
      541 gggagccccc cggatgcgac gggcagactt tgaaaatgtt cgaccagcaa cgtgatggca
      601 atctatacaa gatctggaac cggctatgtt cgggaacatg gtttccgcca ccagtgctgg
      661 aaaaacggat cccgaagtcg aatggcaagg aacgcattct ggggatccca acagtatccg
      721 atcgaattgc tcagggagcg ataaaacttt tcatggaaga aaagcttgac ccgattttcc
      781 atgctgattc atatggttac cgaccaggca aatcagctca tgacgctctg aaacaatgtg
      841 ccatccggtg ctggcgttat agctggatac tggaagtgga catcagtgcg tttttcgacc
      901 atgtgagaca tgatctggtg ctcaaagctc tggaacacca cgggatgcct aaatgggtca
      961 tcctgtactg tcgacgctgg atggaagcgc caatgcagag ttgtgaaaat ggagaattaa
     1021 taacccgaac acgaggcact ccgcagggcg gggtcattag tccgctactt gctaacttgt
     1081 tccttcacta tgcatttgat ttgtggatgg aaagagaata tcggggggta ccgtttgaga
     1141 ggtacgctga cgatattgta gtacattgtt cacgaatgag tgatgcaaca aggctaaaga
     1201 acaggttatc ggagcgcttc tcggaagtcg ggctggtcct gaatgcaggg aaaacgaata
     1261 tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac gagtttcacc tttctcggat
     1321 atgacttcaa agtgcgtacg ctgaagaatt tcaacggcga acggtaccga aaatgcatgc
     1381 cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac aatcaagaag tggcgtatac
     1441 atcgctcaac agctgagagt ttgctggatt ttgcgaggcg ctacaatgcg atagtgagag
     1501 gctggatcga gtactacgga aagttctggt ccagaaattt caactatcga ctgtggagtg
     1561 caatgcagtc acgtctgctc aagtggatgc agtctaaata cagactttcg aaccggaagg
     1621 ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa gctatttgtt cactggtatt
     1681 tactacgtgc atcaaatgag tgatcaagag ccgtatgacg cgagagtgtc acgtacggtt
     1741 ccgtgagcag ccggggagtg aaattctctc cggctgactc gactcgcggg ttccgacagc
     1801 gatgacgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     1861 gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc aggactggcc ggcaaaccgg
     1921 gtacgtgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc aatacggttc
     1981 tgacgagccg cttacttttg gcgtaattct gctgcgcctt tactgaacac aatgccgtat
     2041 tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc tttccattga aaccaaccga
     2101 cgcatattca aaaaagtctg gacgtcctgt tgctccagag tttttattgg tacgggtcgt
     2161 atagttccat gttggcaggc ctcagctatt gcctgtgcgt cgttataatc atttttctgg
     2221 caccgctgat aagctttaac atgttgtgcc ggaagcaata ttacatcatg gtcgagtttc
     2281 ctgatttccc gggcccagta atgagatgcc ccgccagcct ccatcgcaac agtcatttta
     2341 ggctgttgca ccagccagtt aagcagttga ttgcgagaaa gtttggtctt tcctgcgggt
     2401 ttgccatgag cattgatact gaaaagataa aagttcgttt tagccagatc aatgccggtg
     2461 attttctgtt cagacatggt atcccgctta cttttgttgt taaagtttat ccatcatggc
     2521 gcaattgaag gctgccgtga aagaggggga agactatctc atcggttact gttaaacggc
     2581 taagagaaac tatctttttc aagcaaagaa gatacggtga aacaggcgat ttcaccgtga
     2641 aatactgttc aaaaaaaacg ctaataaaag caaaatttct tttaaaacag ccaattaaaa
     2701 actggcgagt tgctggcgaa aaacttgaat tttcacagcg aattcagatt atcttttagt
     2761 acaagagcaa tcatacgatc taacttatcc tgaacttcca acggtaatct gccgaattca
     2821 tatgagaacg tacgtccctt tactcgtttt cgagcaaatt tatctttact atcaaatttc
     2881 cataacaaag ttacaaccgc tttatccttt actcctgaat caagcaatga agacatttcc
     2941 ttggttataa gccgcataat actgttttta acctcgtctt cagaaagatt gtcatcagca
     3001 gtaataagct caatactcgg cgagatattc ttcacaactt catcaataga tatatcaccc
     3061 agcctcagtc gttcacttaa ttcagccagt tgcctgtaat cggcgtatgt cagctcagac
     3121 tgaacaggaa aaagagacac aaaatcttta ggtacggacg ctgcttggag ggcacgagtc
     3181 accttagctg ccgacaatcc ctctctttcc gcaatttgct tctggttcat accggcctct
     3241 ttgagacgta ctaaacgcaa cccaatttca cgaatattat gttcaagtga cgtctgcaaa
     3301 tctttcgcca gatgctgagc ttcagaaaca gtaagctcat catccgttac aagtacacgt
     3361 aatccaacct tgcatagcaa agctgctgcc cgacgacggg aaccatccaa aatctcaata
     3421 agatcgccag tccgaatccc aatacatgga tagaattgtt gtaaacgtat agttctggtg
     3481 atgtctttta gtgagtccgg cgtcagggct gtctgttcgc ggccattcac ctcttcaacc
     3541 acaaaagttt gagtatccac tttgtcatga tcaaccttga tctcagtaaa ttttgccttt
     3601 cggccagtat tgagggtaaa tacatgaact ggagccgata tttcttcagt attattcagg
     3661 atatttgtat tcagcgtacg gccaatagtc ggtcggtgct ttctgttttc catcagtagt
     3721 tggcccttat aaattcaata cgatcaaaaa ctgctttagc gaaatcttct gctgccatac
     3781 gtgcattttt caaagcttca ccactgccaa cataagtaac aggattcgca gatatcacgg
     3841 tatcaaacga ctcgccgctt ctctcaaaac catcaagacg tggcattgaa acatcaagca
     3901 tgtcaccacc gaaaatctct tttgtcaggc tatggcagta tttatgatct gacttattcg
     3961 ccagtttcga catgaagcca atatttgcct gcaaacggca tgaacatcca tcctgttcaa
     4021 taatctgtac aagttcaggt aaacgagcca aatatttaag cgtggaatgg aaatcaactt
     4081 gtgctggcgg caccggtgta aacatgatat ctgcggcagc aatggcattt ttcaaaaatg
     4141 cgtccagatg cgggcctgta tcaataagga tgaagtcgaa atcatgcttc agcttatcaa
     4201 taatattctc acgcaatatc gcgtgtttgt tttgtcctaa tagatgctcc tcgcacagag
     4261 tatcccagtt tgatgcaata aaggcgtcgt caatagaagc cggcataaca taaacgccag
     4321 ggataactga cggaacgata aaatcttcca gtaattcctc acgactcacg ttctgcaaca
     4381 tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg caagtagttc agaaacatag
     4441 tggctgatga ctggggatct aaatcaagga gcagaatacg taaatcttct gacagcaggt
     4501 gaggatgagc acgcagggcg tgagctacgg aaacactact cacggtttta gacacaccgc
     4561 cttttagact accgatgaat atggaatatg cctcactata acgatcgcga tatttaggaa
     4621 tgccacgatg tgcatagata tcgataatgt tctggatggt taatgcatat tttgtcgcgg
     4681 ttcctgctgg gcgtttttca aactgatagc cctgagcctc catctcaccg actgcaccat
     4741 ccacgcttgc gcgcgttaat tttggaagct tagctacagc agactttgaa tagacttgat
     4801 aaaaaacatc agcctgaaac tcctgctttt gctcctgaat ctgttttgtt agggcgagca
     4861 acattttatc tgcacgctgc gcaaccttac ttaactgctc aaaactagtc attccccctc
     4921 cgtgctatgt aggattttaa cttattacaa aaaaaactgc attagcaggg taatgcatgc
     4981 aagtacatat tgtacatgct tatattaaaa atgcgcgcat tgctgattta ttaatcatcg
     5041 gaatgaatat tactgtaaga tagtatgcct ttccgggaga aagacgctga tttcccccga
     5101 ataatccagt tcaccaccac actccggaca acagctttct tcaggctcca gatggtgtat
     5161 ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt gactgtcgca acaggcgggg
     5221 gtagtgcttc caatcagtag aacatgtgct tttcaatgaa ggagccgatg actttttcgt
     5281 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
     5341 ggagggtccg gttgttacgt gcaatacgtt gcgtgaagat tttgccggtc aggtgtttct
     5401 ccttcggcac ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga
     5461 acggagtcaa cagggctaac agttcccgac aggtctcatc attccgtggc ccgaaagtgt
     5521 aagccaacac accgccagtt ttagtgttgt acgcatacca gagccagtgt tgtgtcaacg
     5581 acggatgaaa agtgatccac ttatatctcc accaacggcc caatattgat ccaccgtttt
     5641 actcaggatt agcttctgct ataaccccgg cctttcgttt ctgtctgagt cgatagcttt
     5701 ctcctttgat ttgaacgaca tgtgagtggt gtaagatacg gcccagcatc gctgaggtca
     5761 gtgctgcatc accggcgaac gtttgatccc actgcccgaa cggtagattg gatgtcagga
     5821 tcattgcgct cttttcgtaa cgtttagcga tgacctggaa gaacagtttt gcttcttcct
     5881 gactgaacgg cagatagcct atttcatcaa tgatgagcag gcggggggcc attactccac
     5941 gctgaagcgt cgttttataa cggccctgac gttgtgccgt agataactga agtaacagat
     6001 ctgctgctgt tgtgaagcga actttgatac ctacacggac tgcttcatag cccatcgcta
     6061 ttgccagatg ggttttcccc acacctgatg gccccagtaa tacgatattt tcattacgtt
     6121 ctatgaagct gagtgagcgt aacgactgga gttgcttctg cggtgctccg gtggcgaa
//
LOCUS       DAJTUP010000170         6115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTUP010000170 DAJTUP010000000
VERSION     DAJTUP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.173"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..2334
                     /locus_tag="OO468_003803"
     CDS             <1..2334
                     /locus_tag="OO468_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR6260739.1"
                     /translation="IRQEVKLRDGKVIVRGKVAARRTVKSADIVLYHKPGIPLAVIEA
                     KANKHEIGKGMQQGIEYARLLDVPFVFATNGDGFIFRDATAAEGECLEKQITLDDFPS
                     PAELWQKFCLWKGYTQAQLPVITQDYYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVL
                     LVMATGTGKTYTAFQIIWRLWKSKNKKRILFLADRNILVDQTKNNDFQPFGTAMTKVS
                     GRTIDPAYEIHLALYQAITGPEEDQKAFKQVAPDFFDLIVIDECHRGSASEDSAWREI
                     LDYFSSATQIGLTATPKETHEVSSTDYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDV
                     DLQGWRPTKGQTDLNGEVIDDRIYNQKDFDRTMVIDERTELVARTITDYLKRTNPMDK
                     TIVFCNDIDHAERMRRALVNLNPQQVKKNDKYVMKITGDDEIGKAQLDNFINPKKPYP
                     VIATTSELMTTGVDAKTCKLVVLDQNIQSMTKFKQIIGRGTRIDERYGKLWFTILDFK
                     KATELFADERFDGIPEKVMDTTPEDIADPESDFEEKLEEISEHDDEQVTGVDEPPAPP
                     YQVTDTDDVGPFPEEDEKKIRKFHVNGVAVGVIAQRVQYYDADGKLVTESFKDYTRKT
                     LLKEYASLDDFTRKWQDADRKEAIIHELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYG
                     QPPLTRKERAENVHKRNYFTKYSEAAQAVLDNLLDKYADAGVQEIESIQVLKLKPFDS
                     MGTLPEIIKTGFGDRNGYNQALSELENEIYQLPPRSA"
     gene            2401..3870
                     /locus_tag="OO468_003804"
     CDS             2401..3870
                     /locus_tag="OO468_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR6260740.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            3870..5531
                     /locus_tag="OO468_003805"
     CDS             3870..5531
                     /locus_tag="OO468_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR6260741.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            5752..6093
                     /gene="symE"
                     /locus_tag="OO468_003806"
     CDS             5752..6093
                     /gene="symE"
                     /locus_tag="OO468_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR6260742.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
BASE COUNT         1804 a         1447 c         1431 g         1433 t
ORIGIN      
        1 atcagacagg aggtcaaact ccgggacggt aaagtcatcg tacgtggtaa agttgcggca
       61 cgcagaacgg taaaatctgc tgacatcgtg ctgtaccaca agccaggcat tcccttagcc
      121 gtgattgaag caaaagccaa caaacatgaa attggcaaag ggatgcaaca gggcattgaa
      181 tatgcgcgcc tgctggacgt tccttttgtt tttgccacca acggtgatgg ctttatcttc
      241 cgcgatgcca ccgcagccga aggtgaatgc cttgaaaagc aaataacgct ggatgacttc
      301 ccctcccccg ctgaactctg gcaaaaattc tgcctctgga aaggatacac acaagctcag
      361 cttccggtga ttactcagga ctattacgac gatggcagcg gcaaatcgcc tcgttattat
      421 caacttcagg caatcaacaa aaccattgaa gccgtctcca acgggcaaaa ccgcgttctg
      481 ctggtcatgg cgaccggaac agggaaaacc tataccgcat tccagatcat ctggcgcctg
      541 tggaaatcaa aaaataaaaa acgcattttg ttccttgccg atcgcaacat tctggtcgac
      601 caaaccaaaa ataatgattt ccagccattt ggtacggcaa tgaccaaagt cagcggacgc
      661 accattgatc ccgcttatga aattcacctc gcgctctatc aggctataac tggcccggag
      721 gaagaccaaa aagcgtttaa acaagtcgca ccagatttct tcgatctgat cgtgatcgac
      781 gaatgccatc gcggcagcgc atccgaagac agcgcctggc gagagatcct tgattatttc
      841 agttccgcca cccaaattgg cttaaccgcc acgccaaaag agacgcatga agtctccagc
      901 acggattact tcggcgatcc ggtttacgtc tactcactaa aagaagggat cgaagacggc
      961 ttcctcgctc cttataaagt tgtccgtgtt gatattgatg tcgacctgca aggctggcgc
     1021 ccaaccaaag ggcaaactga cttaaacggc gaagtgattg acgatcgtat ctataaccag
     1081 aaagatttcg atcgcacgat ggtgatcgac gaacgcacag aactggttgc cagaaccatt
     1141 accgactacc tcaagcgcac caatccgatg gataaaacca tcgtcttctg taacgacatc
     1201 gatcatgcag aacgtatgcg ccgcgccctg gttaatctca acccgcagca ggtgaaaaag
     1261 aacgacaaat acgtcatgaa aatcaccggc gatgatgaaa ttggcaaagc tcagttggat
     1321 aacttcatca acccgaaaaa accgtacccg gttatcgcga ccacttcaga gctgatgacc
     1381 actggtgtgg atgctaaaac ctgcaaactg gtagtactag accagaacat ccagtcgatg
     1441 accaaattca agcaaattat cggtcgtggt acacgcatcg acgaacgtta cggcaaactc
     1501 tggtttacca tcctcgactt taaaaaagcc accgaactgt ttgccgatga gcgtttcgat
     1561 ggcattcccg aaaaagtcat ggataccaca ccagaggata tcgccgatcc agaatctgat
     1621 tttgaagaga aactcgaaga aatcagcgaa catgacgacg aacaggtaac aggcgttgat
     1681 gaaccgcctg cgccaccata ccaggttacc gataccgatg atgtcggccc atttccggaa
     1741 gaagacgaga agaaaatccg caagtttcac gtcaacggtg tagcggtggg cgttattgca
     1801 cagcgcgttc agtattacga cgccgacggt aaactggtta ccgaatcctt taaagattac
     1861 acccgcaaaa cactgctcaa agaatatgcc tcgctggatg actttacccg caagtggcag
     1921 gacgccgatc gcaaagaagc gatcattcac gagctggagc aacaggggat catctgggaa
     1981 gtactggcag aagaagtcgg taaagatctc gacccgttcg acatgctttg ccacgtagtg
     2041 tatggtcagc cgccgttaac ccgcaaagag cgcgccgaga acgtgcacaa gcggaactac
     2101 ttcacaaaat actctgaagc agcgcaagcc gtgctcgata atctgctgga taaatacgcc
     2161 gatgcgggcg tacaggagat cgaaagtatt caggtgctga aacttaaacc attcgacagc
     2221 atgggcacct taccggagat tattaaaacc ggatttggcg accgtaacgg gtataatcag
     2281 gcgctcagcg agctggaaaa cgaaatctac caattaccgc cccgctctgc ttaacaactc
     2341 tcttttgcgc cttctgcaac catcatgttg cagaaggcct tgtttttcat ggaatacaac
     2401 atgtctatca gctcagtaat caaatcatta caagatatta tgcgcaaaga tgccggtgtg
     2461 gacggcgatg cgcagcgtct cggtcagctc tcctggctgc tgtttttgaa aatcttcgat
     2521 gcccaggaag aggcgctgga actggagcag gataactatc aatatccgat cccacagcgt
     2581 tatttgtggc gcagttgggc cgccaacgcg cagggcatta ccggtgattc cctgctggaa
     2641 ttcgttaatg atgatctgtt tccggcgttg aaaaacctca ctgcgcctat cgataaaaac
     2701 ccacgcggct acgtggtgaa gcaggcgttc agcgatgcct ataactatat gaaaaacggt
     2761 acgctactgc gccaggtgat caacaagctg aacgaaattg actttaccag cgccagcgaa
     2821 cgccatctgt ttggtgatat ttacgaacag atccttaaag atctgcaatc tgcgggcaat
     2881 gcgggcgaat tctatactcc acgcgccgtc actcgcttta tggtggatcg cgttgatccg
     2941 aaactcggcg aatccattat ggacccggcc tgcggtacgg gcggttttct tgcctgcgcg
     3001 ttcgatcatg taaagaacaa atacgtgaag agcgtcgccg atcatcagac actgcaacaa
     3061 cagatccacg gtgttgagaa aaaacagctt ccgcacctgc tggcgaccac caatatgctg
     3121 ctgcacggca ttgaagtacc ggtacaaatt cgtcacgaca acaccctgaa taaaccgctt
     3181 tcctcctggg atgagcaact ggatgtcatt gttaccaacc cgccgtttgg tggcacggaa
     3241 gaagacggta ttgagaagaa ctttccggca gagatgcaaa cccgcgaaac ggcggatctg
     3301 tttctgcaac tgattgtgga agtactggcg aaaaacggtc gtgcggcggt ggtattgccg
     3361 gatggcacac tatttggcga aggtgttaaa accaaaatca aaaagctact taccgaagag
     3421 tgcaacctgc ataccatcgt gcgtttaccg aatggtgtgt ttaatcccta taccggcatt
     3481 aaaaccaacc tgttgttctt taccaaaggt cagccaacca aagagatttg gttctatgaa
     3541 catccgtatc cggcgggcgt gaaaaactac agcaaaacca agccgatgaa gtttgaagag
     3601 tttcaggcgg agatcgactg gtggggtaac gaggccgatg gttttgccag ccgcgtcgag
     3661 aatgagcaag cgtggaaagt cagcattgat gacgtgattg cgcgcaactt caatctggat
     3721 attaaaaacc cacatcaggc ggaaaccgtc agccatgatc cggacgaact gttagcgcag
     3781 tatgcaaaac agcaggcgga gatccagacg ctgcgtaatc aactgcgcga tattcttggc
     3841 gctgcgctgt ctggcaagga ggttaactaa tgagtgtgga aaagctgatc gttgatcata
     3901 tggaaacctg gacctcggcg ttgcaaaccc gttccaccgc cgggcgcggc agctccggta
     3961 agattgattt gtatggtatt aagaaattgc gtgagctgat tctggaactg gcggtgcgcg
     4021 gtaaactggt gccgcaggat ccgaatgatg aaccggcgtc ggacctgctg aagcgtattg
     4081 cggcggaaaa agcagagctg gtgaagcaag gcaaaattaa aaagcaaaaa ccactgccag
     4141 aaattagtga ggaagagaag ccgtttgaat tgccggaggg atgggagtgg gtgcacctcc
     4201 ctgatattta ttgctcaatt tctgagtcat ctagaaaaat aaaatcctcc gaaatactac
     4261 ctgagggaaa atatcctgtt atcgagcaaa gccaagaatt tatttctggt tattgcaaca
     4321 atgaatgtct gttaataaag ttaaataatc cagttatagt ttttggtgat cacacaagga
     4381 atattaaatt tattgatttt gattttgttg tcggcgctga cggtgtgaag attttatcac
     4441 ctatattaat ttgtgagcgt ttcttttttt ggcagttacg atcatttaaa ttagatgtaa
     4501 gaggatacgc gagacatttt aaagtattaa atagctgctt atttgcatta ccccccattg
     4561 cagaacaaga gcgaatagtt gaaaaagtta gctctttgat gtccctctgc gaccaactag
     4621 aacagcaatc cctgaccagt ctggacgcac atcagcaact ggttgaaacc ctgttgggaa
     4681 cgcttacaga cagccaaaac accgcggaac tggctgaaaa ctgggcgcgt attagcgagc
     4741 atttcgacac cctatttacc accgaagcca gcgtggatgc gttaaaacag accattctgc
     4801 aactggccgt aatgggcaaa cttgtgccgc aggatccgaa tgacgaaccc gcctctgaac
     4861 tgctcaaacg tattgcgcag gaaaaagctc aattggtgaa agaagggaaa ataaaaaaac
     4921 aaaaaccact gccgccaatt agcgatgagg aaaaaccgtt tgaacttccg gaagggtggg
     4981 aatggtgtcg tattggtaat atagttaata ttaaatctga attagtatca ccaaaagatt
     5041 atttaaattt atatcaagta gcacctgata ttattgaaaa gggcactggg agagtgatat
     5101 caaagagaac agtgaaagaa tcaggagtca aagggccaaa tagtcgcttt tataaaggac
     5161 aaattgttta ttctaaaatc cgcccgtcat tatccaaagt ttttttagca gagtataatg
     5221 gtctttgcag tgctgatatg tatccattag actgttacat taatccaaat tacttattga
     5281 aatatatcct ttccattcca tttcttatgc aagtcaaaaa agcagagaac agaataaaaa
     5341 tgccaaaact taattctgac agtttttaca atattattgt tgcgattccc ccctataatg
     5401 aacaacaagc aatatttgat aagataaatt ctattgaagc agtatgcaat ggtcttattt
     5461 cttatattgg gatttatcat aaaacccaac tgcatcttgc agacgcactc actgacgcag
     5521 cgataaacta attccctcgc cctcacaaaa ccaataaagc ggctaaatag ccgctttatt
     5581 cacatcacca aaatttatat ttcacgctaa ttttcatctt attttcatta atttttagct
     5641 ggactttccc tgtatttact gatgatgtat ccaggtattt agcacggtgc ggatgtgcgc
     5701 caacacactc gcaccgctaa tcacaatcac tatttataga gaatagtaat catgactgac
     5761 acgcattcta ttgcacaacc gttagaagca gaagtctccc cggcaaataa tcgtcaatta
     5821 accgtgagtt atgcgagtcg ttacccggat tacagccgta ttcccgccat caccctgaaa
     5881 ggtcagtggc tggaagccgc cggttttgcc accggcacgg cggtagatgt caaagtgatg
     5941 gaaggctgta ttgtcctcac cgcccaaccc gccgccgccg aagagagcga actgatgcag
     6001 tcactacgca aggtgtgtaa gctgtcggcg cgtaaacaaa ggcaggtaca ggaatttatt
     6061 ggggtaatta caggtaaaca gaaagtcgcc tgatacattg acccggtagc gccag
//
LOCUS       DAJTUP010000171         6111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTUP010000171 DAJTUP010000000
VERSION     DAJTUP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.174"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..368
                     /locus_tag="OO468_003807"
     CDS             <1..368
                     /locus_tag="OO468_003807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260743.1"
                     /translation="LLKKRTNGSIRYCQYTSKVAMIYIEQLELIHKSGDVLYPVKITR
                     KSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRI
                     KRTGIYSIKGVNIKEYNVR"
     gene            complement(444..908)
                     /locus_tag="OO468_003808"
     CDS             complement(444..908)
                     /locus_tag="OO468_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR6260744.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(905..1780)
                     /gene="nanK"
                     /locus_tag="OO468_003809"
     CDS             complement(905..1780)
                     /gene="nanK"
                     /locus_tag="OO468_003809"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR6260745.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1777..2466)
                     /locus_tag="OO468_003810"
     CDS             complement(1777..2466)
                     /locus_tag="OO468_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR6260746.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2514..4004)
                     /gene="nanT"
                     /locus_tag="OO468_003811"
     CDS             complement(2514..4004)
                     /gene="nanT"
                     /locus_tag="OO468_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR6260747.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4114..5007)
                     /gene="nanA"
                     /locus_tag="OO468_003812"
     CDS             complement(4114..5007)
                     /gene="nanA"
                     /locus_tag="OO468_003812"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR6260748.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5129..5920)
                     /gene="nanR"
                     /locus_tag="OO468_003813"
     CDS             complement(5129..5920)
                     /gene="nanR"
                     /locus_tag="OO468_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR6260749.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFDEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1534 a         1670 c         1537 g         1370 t
ORIGIN      
        1 ggctgcttaa aaaaagaaca aatggtagta tccgctattg ccagtacacc tcaaaagttg
       61 ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg ttgtatccag
      121 taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca ttcggcttaa
      181 ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg ctggtgattg
      241 atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat aaaaagggca
      301 agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa gaatacaacg
      361 tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca aagatgggtc
      421 aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt caccaccacc
      481 tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc tcctggcata
      541 aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat ggcgctacaa
      601 agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc tgccatgcca
      661 aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg ctcgtgcaat
      721 tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat aaaaatattg
      781 tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc taaggccagc
      841 gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg tacttcaccc
      901 atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct gcatcatggc
      961 ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc agatacattt
     1021 ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc acgcactggc
     1081 aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg gagcgatgaa
     1141 tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt ctcgcatccg
     1201 cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca attgcttcca
     1261 cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc gtatgcccga
     1321 tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact acaccgccgc
     1381 cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc agcgcctgat
     1441 actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa ttggtaagtt
     1501 gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc ggattcagcg
     1561 ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc tgcgcatgag
     1621 cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt gtctggctgg
     1681 ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg
     1741 caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct ttttcatcgc
     1801 tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc
     1861 ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac gcccttcggc
     1921 aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg gctcttctgg
     1981 cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc ccagcttttg
     2041 gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac cgtgatgatg
     2101 gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc cgtcaatggc
     2161 gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg ccgtgatgcg
     2221 taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc tcaccaccgc
     2281 acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc ccgcctgctc
     2341 tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt caggaaccgg
     2401 ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt gtgcaagtaa
     2461 cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca ggattaactt
     2521 ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct aaatggtttg
     2581 ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg aacgcgggaa
     2641 ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga gagcgatgcc
     2701 agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg ggccagtgcg
     2761 ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg gtcggtatcg
     2821 aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag catttgctgg
     2881 aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa taccggaata
     2941 atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc cagccagtca
     3001 ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa gaacagcaca
     3061 ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg cagcagcgcc
     3121 tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat tacgcccgtt
     3181 ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc gcataacagc
     3241 ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa ccacagcgca
     3301 gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg gtagagaata
     3361 tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc ttccgcttcc
     3421 gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat aaagaacagc
     3481 gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac gacggccccc
     3541 acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt tggccagctt
     3601 tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc gatgaccaga
     3661 cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt cccggccgag
     3721 aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc catagcgccg
     3781 agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact tgccgcctgc
     3841 accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat taaaacgaaa
     3901 tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca ttgtgcacgg
     3961 ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag tctcccgcgg
     4021 tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt ttatttcccc
     4081 tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca tcaactgctg
     4141 ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg gtttgcggca
     4201 cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc ggaatacgcc
     4261 cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct gcgcggtctg
     4321 gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca tgatgttgta
     4381 agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga tttcgtcgta
     4441 accgttatag agcaccagat caggatgttc acgacggatc tgttccatct gatagagatc
     4501 gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt tgatctgatc
     4561 cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg gcaaaccatc
     4621 cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga aaggatagta
     4681 gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg ccgcaagttg
     4741 ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct tacctttcgc
     4801 ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta caaaggcttc
     4861 gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga actgaaccag
     4921 gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca ggagtgcagc
     4981 cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg tctgataaac
     5041 gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac agaaagctta
     5101 taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc tgaccgaaag
     5161 cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg gcttcgtcag
     5221 gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta acgttgttat
     5281 gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag tcgagcaggg
     5341 caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg cggtggaaat
     5401 caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg atttccagtg
     5461 cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag cgcaccaggc
     5521 tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga tgagaaagga
     5581 aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt cgagagacac
     5641 gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct gccagcgctt
     5701 cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca gacggtaatt
     5761 gttcaccttc gtcaaattca cgacggcgga tcatctgttc cagctcttct tccaccattt
     5821 cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt gcaggtgaag
     5881 aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt cagtgtgatt
     5941 aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc aggtttttct
     6001 ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa aaattaatgt
     6061 aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag c
//
LOCUS       DAJTUP010000172         6032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTUP010000172 DAJTUP010000000
VERSION     DAJTUP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.175"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(25..2937)
                     /locus_tag="OO468_003814"
     CDS             complement(25..2937)
                     /locus_tag="OO468_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260750.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            2940..3056
                     /locus_tag="OO468_003815"
     CDS             2940..3056
                     /locus_tag="OO468_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6260751.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3420..4262
                     /locus_tag="OO468_003816"
     CDS             3420..4262
                     /locus_tag="OO468_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR6260752.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4265..5353
                     /gene="capU"
                     /locus_tag="OO468_003817"
     CDS             4265..5353
                     /gene="capU"
                     /locus_tag="OO468_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR6260753.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5358..>6032
                     /gene="virK"
                     /locus_tag="OO468_003818"
     CDS             5358..>6032
                     /gene="virK"
                     /locus_tag="OO468_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6260754.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSE"
BASE COUNT         1604 a         1372 c         1414 g         1642 t
ORIGIN      
        1 gttacatcct gtcagcacgg aacctcacgc tttttgagcg gcacgacgga tcccccccac
       61 ccagtatccg acagactttt cactgcgttc gtaaaaaccc ttcttccctc ccagcatatc
      121 cactaattcc ggtgacatat cctgcagata agtctgcaaa tcatgcccca gatcttccgg
      181 tgactgcaac tcataaaccg cgcgccacac atcacccttc cttattttaa gccccgggtt
      241 aagaatatac tgtccgtggt tcagcctcag aaaaagcttt tttccataag gatttttgct
      301 gttaacttca tgttgagaga gctggctgct gatataactc cttcgtttgc ggcgttccgg
      361 cagcaacgct tcaggaagat gaaccacaca ctctgccagt cgtgccgcac tgaaggcttc
      421 cgaaaaaaac agagcacttc ccagatgcgt gtggaacagc gcctgcatca ggatcaccag
      481 aaacattgcc atctgccggt tgtccagttt gatgaggcgt ccttccacct gcagtgacag
      541 actttcaggt ttcagcaggg tatacaactg cgcacttttc tgttgcgcat aacggggcgt
      601 actgaccgcc gctgacagaa cttgatgata agccgccagc ccgtcattac cggtcagcaa
      661 gggatctgca ccacgctcca gcagtaactg aaccagtgca atattacctg cccgggctgc
      721 tgacatcagc ggagtaagat taaacggcga acggtgatca acaccataac gttcaatatc
      781 ccgcaacacg gcaaccgggt tattcattga gtatggccgg aaatacttca gctccagtaa
      841 ctgcagagtc ttcgttctgg gctgccttgt gggcttaaaa tcttcccggt acagtgcatt
      901 atacagcggg aacagggaat aaatcagact gtactccagc gcctgcagtt gtatggtttt
      961 atcggctgta cctgcgagga tatgctgttt ccactgtcgt gcatcctctg cggtgataat
     1021 ctgccatggc atctcagatg tctgcaggat ccggctacgg atggtgtccg cctgctcctg
     1081 cttcccctgt ttttcaagca gatgtgcggt tttctgccac tcatcacggc tggaaatctc
     1141 ttcctgcaga ttcagaggct cttcctgatg cgtgagcgca agcagactcc acagaggatg
     1201 ctccacctgc tgttcaacaa gatacacatt gtgtttagca cgggtgagcg caacatacag
     1261 cgcgttggta aaaaatttat agatttccgc tgaacgatcc tgcctgtccc gcggacggga
     1321 ataacgggcc tcgccctcaa gatcagccgg tgtaagaccc tcgcaaatat catcaaatgc
     1381 ctgccgggca gcgcttacta tattgtaaag gatcaccgtt tcgtattcca gccccttaac
     1441 ctcctgaacc gaaaagacca gcggcgtact gaaccagcat cgcgcctgtg ctttctgttc
     1501 cggatgcatc acaatgaccg ctacacgatt cgaaagtgag gttttggcat tcagttcctg
     1561 ctttgtctct tccctatcgt ccagcaaccg gatggtccct tctgcctgac cgcagcttct
     1621 gacaaaatgg tgactttccc gatcaatagc actaaagcgg acctgtttga gtctcagcaa
     1681 ccggttagcc agtgcagtga cgtggtgccc gttacggtaa ttcgcctgca ggatattaac
     1741 ggttgtgtcg tttccctgtt gctgccggaa aaagagactt ttgagcgaac tccatgagaa
     1801 aaagctcgga tgaactatct gattggcatc accacagagt aaaaactgac cgggatgacg
     1861 tagcgttttc agtaccagtt gcagttgaac aggcgtcata tcctgaactt catcaacaaa
     1921 aatgacatca taacggggag ttgccaacga aagatactga tgactgagca gattactgtc
     1981 atactgatgc gactgtttca gccatgcgat ataacgttca aacaggacat agaccgtcgg
     2041 gcggtcctcc atgccataga gcgactgccg tatccccagt gacagatacg cctcccggga
     2101 cagcggccca ttcccggagg caaccgcacc gatcacgccc ctgaactcct catacagggt
     2161 atgggcagcc cccagtgcgg cgatagccct gttgcggggc agccagtcgc tgaacgcact
     2221 gaaagtcacc tccctgcctt ccggaatgcg cagagtttcc agaaattccg tcagcgaaag
     2281 aaagtcaata ttctgtacct cactcccttc ccctgaagcg tcatagagag tacgcgcttt
     2341 ttccaccaga aatgaggaaa ggctgagata cagtatatca ccagcagcct gcttcatttt
     2401 ttccagtacc agcgccgtct taccacttcc cgccgagcca acaatcacca gcggtagcgg
     2461 gtgttcgtac agtgcctgct gatcatcatc aaaggacagc attttatcca ggcggtggaa
     2521 acgcccatga gacggattga tatacgtcag cgcttcagtc gcgatatcaa caggatccgg
     2581 cactggttgt tgttgtaagc gcccttcatc aatcaccacg tttctgcgaa gaaaacgtga
     2641 agtgttatag gcatgattgc gaatatattc cagaacaagg cagatagttt caccgtggta
     2701 gcgatacagc gaaaacagta atcggtcccg gatactgagc cgggcgcggt aaagattggt
     2761 gtcaatttta cgaacatcag cctgaacaaa gttatcggtg gcaatagcct gtgcaaattt
     2821 tttataaccc gggatcgtgt caggattcag agtatgataa agacagattt tcatggctgc
     2881 cggtttccgt cgtgcaaaaa acatgctgat tttacgaagg aatgccttac tgaacaacct
     2941 tgcccggaat accccccgaa atcagatgga acaatcagaa aaatgctctg cagttatgga
     3001 ataaagaagg tggggtggta cgccgggagg gcgatccgct ggaaatggcg cagtagctgc
     3061 aggcctgtca tgatgcagga atagaagttc gtgtacaggt taatgaaagc gtcaccccgt
     3121 aaataaactc tgcgctgaaa ttcctttttt tcattggagt acctgtgttg ttctgaggtg
     3181 aacatatcac ctctgttcag gtagccaaat tcagtgtgcc acctcaaggt tcttagtgag
     3241 tcgtttttaa aagatatgat aatatatcat tgtttatgaa ttgttgatgt ttaatttagt
     3301 aaattatatc aaatatgcca tgggtttgct atgttattca tccagtatct ggaaataaaa
     3361 tgcaccagct ttattgggtt ttccttttgt ctctgcttgc taggtgccag aaactttaaa
     3421 tgttaaacga agggggcatt ttgttcaaag ccaatcatgt tccggtatta atgtatcatc
     3481 atgtcagtca ttgccccgga ctggttacac tttctcccgt gacattccgg aagcaaataa
     3541 aatggttggc tgaaaacaac tggaaaacac tgtcatcaga tgaactggag tttttttatc
     3601 ggggcggaaa actcccccgg aaaagcgtta tgttgacatt tgatgatgga taccttgaca
     3661 actggtttca ggtatatcct ttattaaaag agtttaatct caaagcacat atttttttaa
     3721 ttaccggttt tattgggaat ggtccggttc gtcactctcc tggaaaggaa tattctcatc
     3781 gcgattgcga acatcaaata gcgacaggga atgctgataa tgttatgctt cgctggtctg
     3841 aagtaaacga aatgttgcaa tctggtctgg tcgaatttca tgttcatacg catactcata
     3901 cacgctggga taaaaagttc agttcacgag aagaacagtg taaacatctt cggcaggact
     3961 tgttgagcgg gagggaatat ttgaaagaaa tgacaggaaa atgcagtaaa catttgtgtt
     4021 ggccggaagg ttattataac aaggattata ttcaggtcgc tgaagaactt ggattctatt
     4081 atttatatac aacagaaaga agaatgaatg ctcctgctaa aggaactaca cggataggaa
     4141 gaataagcac caaagaacgg gaaagttgcg catggctgaa aagacgactt ttttattata
     4201 caaccccgtt tttttcatcg ttgctggcct ttcataaggg gccaagatta ccggatgatt
     4261 aataatgaat atactattta cggaatcatc accgaatata ggtggtcagg aattacaggc
     4321 tgttgctcaa atgaaggccc tgaagaaaat ggggcattca gttctgcttg tctgcaggga
     4381 aaacagcaaa attgcttttg aagccagtaa attggggatt gatatcacat tcgcgttatt
     4441 tcgaaacagt cttcacatcc ctactgcatg gagattactc ggaatagttc atggttttca
     4501 gcccaatgca atcgtttgtc acagtgggca tgatagcaat attgttggtt tagtacggtt
     4561 attcactagg aaacatccat tcagaattat caggcaaaag acatatttga cacgaaaaac
     4621 aaaagttttt tcaataaatc atttttgcga tgaggtgatt gttcccggaa caagtatgaa
     4681 gacacatctg gagcaggaag gatgtcgaac ccgggttact gttgtgcctc caggctttga
     4741 tttccagaaa ttatacgttg attcccgaaa cagtttgcct ccaaatgttc tttcttggct
     4801 ggcgtcccga aggggatgcc ccgttattgc tcaggtagga atgttgcgcc cggaaaaagg
     4861 gcacgaattt atgttgaatt tactgttcca tttaaaaatg aatggacgac agttctgttg
     4921 gttgattgtg gggtctggtt cgcctgaact gcgggagcat ttacagtatc agattgacag
     4981 tatgggcatg catgatgatg tttttattgc tgacaatgtt tttcctgccg cccccgtata
     5041 tcgggttgcc agtctggtgg ttctgccttc agaaaacgaa tcgtttggta tggtactggc
     5101 agaagcatcg gcattttctg tgcctgtact ggccagtcag attggtggaa tccctgatgt
     5161 tattcagaat aaccagaccg ggacattgtt accagcaggt aataagcacg catggatgtg
     5221 cgccctgaat gattttttta atgaccctgg gcgtttttat cagatggctc gccaggcaaa
     5281 acaggatata gaagagcggt ttgatattaa taaaactgcg ttaaaaatac tcacattagc
     5341 gaagcacaag taacaatatg ttttctgtaa gtaacttatc atttatcggt ttccttaaaa
     5401 ggattgtttt ttcctcagat tcactcccgg ggaagtggga acacagaaaa ttccggttca
     5461 tgtacatttt gcgatgtgct ataaatccgg ttgccagtat tcgatattat tacgaactgc
     5521 gttccttgca gtgcattgag gatattctgg caatacaacc cacgttgcca gcaagaattc
     5581 atcgacctta tttgcataaa ggggggcgag cctggtcccg ggggcaatat attcttgagc
     5641 attatcgctt tgtgcagaat ttgccagaaa aatattctga atttttgttt ccacaaaaat
     5701 cggtatcatt agtccaattc atcgggaaag atggagagga ttttgatatc cagtgttctc
     5761 ccagtggttt tgaccgggaa ggagagttga tgttgtcatt gtttttcaat aaaatagtga
     5821 ttgcccggtt aacattttca gtcattctga ctcaaaatgg acacaccgct tttattggcg
     5881 gattacaggg agcccccaaa aataccggac ctgatattat tcggtgcgca acccgggcct
     5941 gttatgggct tttcccaaaa cggattattt ttgaagcatt ttgtgcgttg atgaaagctt
     6001 gtaatgtctc tgagtgtctg gcagtcagtg ag
//
LOCUS       DAJTUP010000173         5995 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTUP010000173 DAJTUP010000000
VERSION     DAJTUP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.176"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1301
                     /gene="narU"
                     /locus_tag="OO468_003819"
     CDS             <1..1301
                     /gene="narU"
                     /locus_tag="OO468_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR6260755.1"
                     /translation="IARRNLWISVSCLLLAFCVWMLFSAVTVNLNKIGFNFTTDQLFL
                     LTALPSVSGALLRVPYSFMVPIFGGRRWPVFSTAILIIPCVWLGIAVQNPNTPFGIFI
                     VIALLCGFAGANFASSMGNISFFFPKAKQGSALGINGGLGNLGVSVMQLVAPLVIFVP
                     VFAFLGVNGVPQADGSVMSLANAAWIWVPLLAIATIAAWSGMNDIASSRASIADQLPV
                     LQRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILRLAFFGPFIGAIARSVG
                     GAISDKFGGVRVTLINFIFMAIFSALLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSG
                     STFQMIAVIFRQITIYRVKMKGGSDEQAQREAVTETAATLGFISAIGAVGGFFIPQAF
                     GMSLNMTGSPVGAMKVFLIFYIVCVLLTWLVYGRRKFSQK"
     gene            1383..5123
                     /gene="narZ"
                     /locus_tag="OO468_003820"
                     /pseudo
     CDS             1383..5123
                     /gene="narZ"
                     /locus_tag="OO468_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5120..>5995
                     /gene="narH"
                     /locus_tag="OO468_003821"
     CDS             5120..>5995
                     /gene="narH"
                     /locus_tag="OO468_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR6260756.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1395 a         1606 c         1605 g         1389 t
ORIGIN      
        1 atattgctcg aagaaacctc tggatatcag tcagttgtct acttcttgcc ttctgtgtct
       61 ggatgctatt tagcgcagtt accgttaatc tcaataaaat cggttttaat tttactaccg
      121 atcaactctt tttattaacc gcattaccct ccgtttctgg cgcattattg cgtgttccct
      181 actcctttat ggtgcctata ttcggtggac gccgatggcc ggtttttagt actgcaatcc
      241 tgattattcc ttgcgtctgg ctcggaattg ccgtgcaaaa tccgaatact ccttttggga
      301 tatttatcgt tatcgctttg ctatgcggtt ttgcaggtgc aaactttgct tcgagcatgg
      361 gcaatatcag tttcttcttt ccaaaagcca aacaagggag cgctcttggg attaatggcg
      421 gattaggaaa cttaggtgta agtgtaatgc agctggttgc accgctggtc atttttgtac
      481 ctgtatttgc ctttctcggc gtcaatggcg taccgcaggc cgacggttcg gtgatgtcgc
      541 tggcgaatgc cgcatggatt tgggtaccgc tactggcgat tgccacgatc gccgcctggt
      601 cagggatgaa tgatatcgcc agttcacgcg cctcaattgc cgaccagctc cctgtcttac
      661 aacgcctgca tctctggctg ctgagcctgc tttaccttgc caccttcggt tcgtttatcg
      721 gtttttctgc gggttttgcc atgctggcaa aaacccagtt cccggatgtg aatattctgc
      781 gcctggcgtt ctttggccca tttattggtg ccatcgcgcg gtcggttggt ggtgctattt
      841 ccgataaatt cggcggcgtg cgggtgacgt tgatcaactt tatttttatg gcgattttca
      901 gtgccctgct gttccttacc ttaccgggca caggctccgg taatttcatc gccttttacg
      961 ccgtatttat ggggctgttt ctgaccgcgg gtctgggaag tggttctact ttccagatga
     1021 tcgccgtcat ctttcgccag ataaccattt atcgggtgaa gatgaaaggc ggtagtgatg
     1081 agcaagctca gagagaagcc gtcaccgaaa cggcggcaac gctgggcttt atctcagcca
     1141 ttggcgcagt gggcggcttt tttattccgc aggcatttgg catgtcgctc aatatgaccg
     1201 gctctccggt cggcgcgatg aaagtgtttt taatcttcta catcgtttgt gtgctgctga
     1261 cctggctggt ttatggtcgg cggaagttca gccaaaaata agagatcgat gcctgatgta
     1321 caaccatgtc gcatccgaca aggcatcaga tcccaattca ttcctggagc aggagtcatg
     1381 tcatgagtaa acttttggat cgctttcgct acttcaaaca aaagggcgaa acctttgccg
     1441 atggtcacgg acaggtgatg catagcaacc gcgactggga ggacagctat cgccagcgtt
     1501 ggcagttcga caaaatcgtg cgttccaccc acggcgttaa ctgtacaggc tcctgtagct
     1561 ggaaaatcta cgttaaaaat ggtctggtga cctgggaaat ccaacagacc gactacccgc
     1621 gcactcgccc tgacctgccc aatcatgaac ctcgcggctg cccgcgtggc gcaagttact
     1681 cctggtatct ttacagcgct aaccgcctga aatacccgct cattcgtaaa cgactgattg
     1741 aactgtggcg cgaagccctc aaacaacaca gcgatccggt actggcgtgg gcatcgatta
     1801 tgaatgatcc gcaaaagtgc ctgagctaca aacaagtgcg tgggcgcggc gggtttatcc
     1861 gctccaactg gcaggaacta aaccagctga ttgccgccgc taacgtctgg accatcaaaa
     1921 cctacggccc ggatcgcgtt gccggtttct cgccgatccc ggcgatgtcg atggtttctt
     1981 acgccgccgg aacgcgttat ctgtcgctgc ttggcggcac ctgttaaagt ttctacgact
     2041 ggtattgcga cctgccgccc gcctcgccga tgacctgggg cgagcaaacc gacgtaccgg
     2101 aatctgccga ctggtataac tccagctaca tcatcgcttg ggggtctaac gtaccgcaga
     2161 cacgtacgcc ggacgcccac ttctttaccg aagtacgcta caaaggcact aaaaccatcg
     2221 ccattacccc tgactactct gaagtggcca aattgtgcga ccagtggctg gcaccgaaac
     2281 aaggcactga tagcgccctg gcgatggcaa tggggcatgt gattttaaaa gagtttcatc
     2341 tcgataatcc cagcgactac tttatcaact actgccgccg ctacagcgac atgccgatgc
     2401 tggtaatgct ggagcctcgc gacgatggta gctacgttcc cgggcgcatg atccgcgcat
     2461 ctgacctggt ggatggactg ggcgaaagca acaatccgca gtggaaaacc gtagcagtta
     2521 ataccgcagg tgaattggta gtgccgaacg gttcgattgg tttccgctgg ggagaaaaag
     2581 gcaaatggaa tctggaatcc attgccgccg gtacggaaac cgaattgtcg ttaaccctgc
     2641 tcggtcaaca tgacgctgtt gcaggcgtgg ccttccccta ctttggcggc attgaaaatc
     2701 cgcattttcg cagcgtaaaa cacaatccgg tgctggtgcg ccaattgccc gttaaaaacc
     2761 tgacgttagt cgatggcaac acctgtccgg tggtcagcgt ttatgatttg gtactggcga
     2821 attacggcct cgatcgcggg ctggaagatg aaaacagcgc gaaagattac gctgaaatca
     2881 aaccgtacac cccagcctgg ggtgagcaaa ttaccggcgt accgcgccag tatattgaaa
     2941 ccatcgcccg cgaatttgcc gataccgccc ataaaacgca tgggcgctca atgattatcc
     3001 tcggcgcagg tgttaaccac tggtatcaca tggacatgaa ctaccgcggg atgatcaata
     3061 tgctgatctt ctgcggttgt gtcgggcaaa gcggtggcgg ctgggcacac tatgtcggcc
     3121 aggaaaaact gcgcccacaa accggctggt tgccactggc ctttgcgctc gactggaacc
     3181 gaccaccgcg ccagatgaac agcacctcgt ttttctacaa tcattccagc caatggcgct
     3241 atgaaaaagt ctctgctcag gagttacttt caccgctcgc cgatgccagt aagtacagcg
     3301 gtcatctgat tgatttcaac gttcgcgccg aacgtatggg ctggctacct tctgcgccgc
     3361 agctggggcg taacccgctc gggattaaag ctgaagccga caaggccgga ttatccccca
     3421 cagaatttac cgcccaggcg ctgaaatcgg gcgatttacg tatggcctgc gaacaaccag
     3481 atagcggcag caatcatccg cgtaatttgt ttgtctggcg ctctaacctg cttgactcct
     3541 ccggcaaagg ccacgagtat atacagaagt atctgctggg gaccgaaagc gggattcagg
     3601 gcgaggaact cggtgccagc gacgggatca aaccggaaga agtcgagtgg caaactgcgg
     3661 cgattgaagg caagctcgac ctgctggtga cgctcgactt ccgcatgtcc agtacctgcc
     3721 tgttctccga tatcgttctg cccaccgcca cctggtatga aaaagacgat atgaacacct
     3781 cggatatgca tccatttatt catccgcttt ctgcggcggt cgatccggcc tgggaatcac
     3841 gcagcgactg ggaaatctac aaaggtattg ccaaagcatt ttcgcaagtg tgcgtgggcc
     3901 atcttggcaa agaaaccgac gtggtattac aaccactgct gcatgactct ccggcagagc
     3961 tctcacagcc gtgtgaagtg ctcgactggc gcaaaggcga atgcgatctg atcccgggta
     4021 aaaccgcgcc gaatattgtg gcggtggagc gcgactaccc tgctacgtat gaacgcttta
     4081 cctcgctcgg gccattgatg gacaaacttg gcaatggcgg taaagggatt tcgtggaata
     4141 cccaggatga aatcgatttc ctcggcaaac tcaattacac caagcgtgat ggcccggcac
     4201 aggggcgtcc gctgattgac accgccattg acgcttcaga agtgattctg gcactggcac
     4261 cggaaaccaa cggtcatgtt gcagtcaaag cgtggcaggc gctgggcgag atcaccgggc
     4321 gcgaacatac ccatctggcg ctgcacaaag aggacgagaa gattcgcttt cgcgatattc
     4381 aggcgcagcc gcgtaaaatt atctccagcc ccacatggtc cggtctggaa agcgatcacg
     4441 tatcctataa cgcgggatac accaacgttc atgagttaat tccgtggcgc acgctgtcgg
     4501 gacgccagca gctctatcag gatcatccgt ggatgcgtgc ttttggtgaa agcctggtgg
     4561 cttatcgccc gcctatcgac acccgtagcg tcagtgagat gcgccagata ccgccaaacg
     4621 gcttccagga aaaagcactt aacttcctga cgccgcacca gaaatggggc attcactcaa
     4681 cctacagtga aaacctgcta atgctgacgc tctctcgcgg tggaccgatt gtctggatca
     4741 gcgaaacaga tgcccgagaa ctgaccattg tcgataacga ctgggtggaa gtatttaacg
     4801 ccaacggcgc gctgactgcc cgcgcggtgg tcagccaacg tgtaccgccg ggcatgacca
     4861 tgatgtatca cgcccaggaa cgcattatga atattcctgg ttcggaagta actggcatgc
     4921 gcggcggcat tcataactcg gtcacccgcg tttgcccgaa accgacgcat atgattggcg
     4981 gttacgcgca gctggcctgg ggctttaact actacggcac cgtcggttcg aaccgcgacg
     5041 aattcatcat gatccgcaag atgaagaacg ttaactggct ggatgatgaa ggtcgcgatc
     5101 aggtacagga ggcgaaaaaa tgaaaatacg ttcgcaagtc ggcatggtgc ttaacctcga
     5161 taaatgtatc ggctgccata cctgttcggt gacctgtaac aacgtctgga ccggacgcga
     5221 aggcatggag tacgcatggt ttaacaacgt cgaaaccaaa ccgggcattg gttatccgaa
     5281 aaactgggaa gatcaggaag agtggcaagg cggctgggtc cgcgatgtga atggcaagat
     5341 ccgtccgcgt ctgggcagca agatgggcgt aataaccaaa atcttcgcca acccggtggt
     5401 gccgcagcag attgatgatt actacgaacc tttcaccttc gactacgaac atttgcatag
     5461 cacaccggaa ggcaaacata tccctactgc tcgcccgcgt tcactgattg acggcaaacg
     5521 gatggacaaa gtgatctggg gcccaaactg ggaagaactg ctgggcggcg agtttgaaaa
     5581 acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag atgtacgggc agtttgaaaa
     5641 caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gctgcgtggc
     5701 gacctgccca agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca
     5761 ggataaatgc cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt
     5821 caactggaaa agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg
     5881 ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct
     5941 ttacgacgcc gaccgcatcg aggaagcgtc gagcaccgag cgcgaagttg acctc
//
LOCUS       DAJTUP010000174         5972 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTUP010000174 DAJTUP010000000
VERSION     DAJTUP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.177"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..382)
                     /locus_tag="OO468_003822"
                     /pseudo
     CDS             complement(1..382)
                     /locus_tag="OO468_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(568..3876)
                     /gene="icsA"
                     /locus_tag="OO468_003823"
                     /gene_synonym="virG"
     CDS             complement(568..3876)
                     /gene="icsA"
                     /locus_tag="OO468_003823"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR6260757.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4405..5607
                     /gene="virA"
                     /locus_tag="OO468_003824"
     CDS             4405..5607
                     /gene="virA"
                     /locus_tag="OO468_003824"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR6260758.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5786..>5972
                     /locus_tag="OO468_003825"
     CDS             5786..>5972
                     /locus_tag="OO468_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6260759.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1838 a         1352 c         1015 g         1767 t
ORIGIN      
        1 gcttccccat gtgatttgcc tcgccacttc accgggctca ggaccccgta ggatcttcac
       61 cgaaaaaaat gcatcaccag ttgctttatt atttgtttta ggtagacctg ttactaattt
      121 atacctactc tgaccaagtc tggataagaa aggcacacct gcacatttct acttggaaat
      181 gttttctttt accattttgc tcaaaaattt caacatccaa tattcctcgt acagaactac
      241 tcaagtcctg aatagaaaaa gttccagctg gtacagtaga gtcatatatt actcgacctt
      301 gttgagatac aacaacacgt gcattagttt ctgcaatacc agtaatctga ggcgcataac
      361 cacgaatatt tggcggtagc attcgttcat cgctttctaa tgcaattcct gtgtaacgcc
      421 aagcaacagc gcattctggc gcatgatctg gtaaacacgt ttggcattga tcgcaggcat
      481 accatcatgt gcacaaaaac ccaccgagct tttacacggt gggtcccaga gaaatgcagg
      541 acatcaacac gccctgcatt tttattatca gaaggtatat ttcacaccca aaataccttg
      601 ggtgtctctg taactgttat tacccaattg ctgagatacg tttccccaga tgcttaggtt
      661 atctgcaaga cgccccccca caccagtttt gagttcaatc acattacggt tgcctatctg
      721 gtgatttgac atgccattca taattacacc atattgcttt gagctccatt tccaattcac
      781 ttccaaaaat ggttcgaact tacgaagagt ctccttatcg atacaactct gactaattaa
      841 ggatgtacgc aatcccaatt ttgcttgaat attattgccc ccagaggatg tgaccatagt
      901 gccattgtgt tcaatatagt cgtttgcttt taccccatgc ctagttatct ggacttgtgg
      961 attgagatac aatgcgttgt caatattatt atgagcagtc cagcgtatgg ttgggtagat
     1021 atagccagct tccaaagctc ctgttattcc tttgctggaa tatttctcac cagttaaccc
     1081 atctcctttg actgtattat taaaccaact gtactgcaac caagcatcca tatatagacc
     1141 tgttcgttct ttttcatcct gaaaccaaga actgtacaaa ccggcagtat aaccgctcac
     1201 agttcctcgt gaactataac tagtatactt attatgagtt ttgctatcct gatatccata
     1261 agctcccata atacccacat gcaagccatc atgatcagtg aagtttgtct tcaaaatatc
     1321 tccaccaatc tgataaacca tcctgttagc tgtggttttt aattgtctat cagctaaatt
     1381 atgctcatta tgtccaccaa cagtacgaat ccacatggtg gtttcgctga gctgacctgt
     1441 agtaggatca acaatagtgc taccgtgtcg gtcatagaga gaaggactaa acatcgtgtt
     1501 cattgctatc aggttagcaa tataactgcc agcttcaggg cgccatgtgt gaatactacc
     1561 ttcacccaaa ttaagtgaag atacaagctg tgaactagct cggcgactac tcatttgagt
     1621 agactcttga ttgctcatct gttttgattc ttgattatcc atctgactag ttagatacca
     1681 cttattggta ttcagtccag atacagtgcc ctgtttcagg cgatagtcat aactaccagc
     1741 aacaatacgg cctttctgaa taaaagcatt cttatcagaa gaatctgtcg aaataatttg
     1801 aacaccttca agagttttat ctccagagcc tccctcattt gtcactgcaa cataggtaat
     1861 accagacgca tggcctgcaa tttccagccg gtcagtttta ctgttgtcat tgccgagaac
     1921 cgcgcccaaa gataatattc cgccctttcc atgccaatta cctttctgaa cataggtctg
     1981 accgacattt gacgaactat tattgagaat aacatgaccg tttttctcca gagtcatgtt
     2041 acctgtgaca atgacggggt caggcaaaaa aggggcatta atattattga ttagcacagt
     2101 cccactattt aataaagtgt taacagtttg gttcatgcct gaaaaattca aggtagcacc
     2161 tttattaaca ataacaccag cggtacgtgt catagcttca actgtcccca tttttagaat
     2221 gcctgcattg atgttggtga aaccagagta ggtatttttt tccgccaaaa tgagagttcc
     2281 ctcccctgat tttgttaggg attttccgtc ccagctattt atattatgat ggctgagatt
     2341 atctgccaga atagtactaa cagtaagttc agcgttttct ttcagattga actctccatg
     2401 actgtcgcca tctgtatcat tccagctcaa accgtagatc aatttattct ggtcactgat
     2461 atagcttttt gtatagtcaa tagcaccagt agtgagtaac ggattgatga tagatatatt
     2521 agaaacactg aaacctgtta tccctttatc tgcacttagc agtttatagt ccttctgcgc
     2581 ccagttattt ataatagcct ccacttttac attgttgcca aatgacatat ttccagaagc
     2641 ttccagacta ctgattccag ctaaatttaa agaactgttc tcaccaaaag aaacgtcacc
     2701 tgaaacagtg aatttgttac ctgatagtga aacagttgta caatcaccag cacataaatc
     2761 accttcaata gttggtgtgg tcccagtgta atcattgttg ataattttca gaatggatga
     2821 attgttaacc tgtacatcac cagtaatgac agaaccttca tggagtatta atgaattgtt
     2881 tccaccagtg atgtggatag cattacctgc tacattttgt gttccatcat cttctttacc
     2941 tgaaattata tatccgctat tagcaatggt catattgcta ccatttatag catcccccca
     3001 atgtccacca ttaccgccta aaatagctcc attgttgatt acagacaggt tatctcctgt
     3061 gatagcatta ccaccgtagc catcataacc ataaccgtaa gcagcacctc cgttgcctcc
     3121 agcgaacgtg cccttattga ttacagagag gctgcttcct gtgatggcat cacctccatt
     3181 accgccatca ccctcaccat aattgttacc tccattacca ccggaaatag tccctttgtt
     3241 gattatgggt aaattgactc ctgtgacggc atcacctcca ttccccccat cactatcact
     3301 ataactatcg ccaccatgtc cacctgaaat aatttctcca tttattataa acagattgtc
     3361 tcctgtaaca gcctcaccac catcaccgtt atggtcagca ccgctaccgc cgctaccacc
     3421 aagaatcatg ccttgattga ttatagacaa gtcagatccg gtaatagagt caccaccatt
     3481 accgccacaa ctattaccat cattattatc accagcacca ccatgacctg cgctaatcat
     3541 aatatcagag ttaatccgta gcttagttct agatgcatga gaggggttgg aagaagttat
     3601 ataccacgca tccattccat cttcaccagc tcccaaggga gaaagtccat caacaggtgt
     3661 taataatttt tcataattgt cctctgaaaa atgaagttct tgagtacccg aaagaggagt
     3721 agcaaaagct attggccccc cgagcaacag ggatgcacca acaacaaaag gagaaaaaga
     3781 caatttgcat gttttttcct ttaccgtagg taattctccg gcccccccct gtgaacattg
     3841 ggtcatatta caaaaaaatt tgtgaatttg attcatgcac tatattatca gtaagtggtt
     3901 gataaacccc tgaaaagatt ccgttttatc cggaataaag ggacgatata tgcaaaaaca
     3961 tattaaacaa agcctcaacc aaaacatgag agctgtgtta tatatcaatg caacgggaga
     4021 aatttacctg tgtcttgctt tgaaaaaaag agggggaaag ctatatgttc gataataaat
     4081 taataatttt actatgatag tctgcttcag accgcaactt ttcagttctt tctgtacgct
     4141 tgcccaaagt ttaaccactt tgtttttcta tacacccaat ggttctatct gccccttgat
     4201 gttaatcccc aaagccaata acatgaatgt attcatatca caactttcct accagactct
     4261 ccaatgcaat aactgcataa ttgtttttag tggaaatgta tcgttttctt aaagagaaga
     4321 ataacattcc attttattat gttcccaatt taattatatt aatatatatt ggcattaata
     4381 ggaaaataca tcaggagaaa tcaaatgcag acatcaaaca taactaacca tgaaagaaat
     4441 gactcatcat ggatgtcaac tgtcaaatca actacagaag taagttggaa taaattgagt
     4501 ttttgtgaca ttttactgaa aataataact ttcggcatat attctccaca cgaaactcta
     4561 gccgaaaaac atagcgaaaa aaaactaatg gatagtttct caccatcact atcacaagat
     4621 aaaatggacg gagagtttgc ccacgcaaac atagatggca tttcaatacg gctatgcctt
     4681 aacaaaggca tatgttcggt tttttatctt gatggagata aaatacaatc cactcaactt
     4741 tcatcaaaag aatataataa cttgttaagt tcactccccc ccaaacaatt caacttagga
     4801 aaagtacata ctattacagc tccggtatca gggaatttta aaactcacaa gcctgcacca
     4861 gaagttattg aaacagccat taattgctgt acatcgataa taccaaacga tgattacttc
     4921 cacgttaaag acactgattt taatagtgtt tggcatgata tttacagaga tattcgagca
     4981 tctgacagca atagtacaaa aatatatttc aacaatattg aaataccact aaaattaatt
     5041 gcagatctaa ttaatgagtt aggaattaat gaattcattg atagcaaaaa agagcttcaa
     5101 atgctaagtt acaaccaagt aaataaaata atcaattcaa acttccccca acaagaccta
     5161 tgttttcaaa cagaaaaact tttgtttact tcactctttc aagaccctgc atttatatct
     5221 gcactaacat ctgcattctg gcaatctctt cacatcacgt cttcctctgt ggaacacata
     5281 tatgcccaaa ttatgtccga aaacatagaa aacaggctta atttcatgcc tgaacaacga
     5341 gttattaaca attgtggaca tattataaaa atcaacgcag ttgtccctaa aaacgacact
     5401 gcaatctctg cctctggggg gagggcttac gaagttagct catcaatatt accatcgcac
     5461 ataacatgta atggtgttgg tattaataaa atagaaacaa gctatctcgt tcatgctgga
     5521 acacttccaa gcagtgaagg gcttcgcaat gctatccctc cagaaagtcg tcaagtatca
     5581 tttgccatca tatctcctga tgtttaagcc aatattatat tggctgccag actgtaaaaa
     5641 aacgttcttc agaacagctc tataaactat taaaccaaag ctaaaaggat ggggaccaat
     5701 agaatctcag cccatccttt ttatatcatt atgaaccgcc ccggaaatcc tggagactaa
     5761 actccctgag aaagaggtaa acaggatgac taaaaataca cgtttttccc ctgaagtccg
     5821 tcaacgggca gttcgtatgg ttctggaaag tcagggcgaa tatgactcac aatgggcagc
     5881 gatttgttcc attgccccaa agattggctg tatactggaa actctgcgtg tctgggtacg
     5941 ccagcatgag cgggataccg ggggcggtga gg
//
LOCUS       DAJTUP010000175         5735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTUP010000175 DAJTUP010000000
VERSION     DAJTUP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.178"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1210
                     /gene="celF"
                     /locus_tag="OO468_003826"
                     /gene_synonym="chbF"
     CDS             <1..1210
                     /gene="celF"
                     /locus_tag="OO468_003826"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR6260760.1"
                     /translation="DIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQ
                     LPARELDERIPLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTN
                     PAGMVTEAVYRHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIK
                     DVLVNGKSRFAELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQK
                     EMLAIEMGEYYKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEV
                     INAIYNDKQAEHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGL
                     IHTIKGFEIAASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFA
                     DCIAELKKAH"
     gene            1223..1981
                     /gene="chbG"
                     /locus_tag="OO468_003827"
     CDS             1223..1981
                     /gene="chbG"
                     /locus_tag="OO468_003827"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR6260761.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2028..4289)
                     /gene="katE"
                     /locus_tag="OO468_003828"
     CDS             complement(2028..4289)
                     /gene="katE"
                     /locus_tag="OO468_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR6260762.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4472..4735
                     /gene="cedA"
                     /locus_tag="OO468_003829"
     CDS             4472..4735
                     /gene="cedA"
                     /locus_tag="OO468_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR6260763.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5012..5735
                     /locus_tag="OO468_003830"
                     /pseudo
     CDS             5012..5735
                     /locus_tag="OO468_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1452 a         1240 c         1456 g         1587 t
ORIGIN      
        1 ggatattatt ttcgatctct gccaacggat gattgataac gctggcgtcc cgatgaagct
       61 ttataaaacg ctggattgcc gcgaagcatt gaaagatgct gatttcgtta ctacccaact
      121 gcgcgttggc caattaccgg cgcgtgaact ggatgaacgt attccattaa gtcatggtta
      181 tcttggtcag gaaaccaacg gcgcgggcgg tttgtttaaa ggtctgcgta ccattccggt
      241 gatttttgac atcgtaaaag atgtcgaaga actttgtccg aatgcatggg tgattaactt
      301 cactaacccg gcgggaatgg ttactgaagc cgtttatcgt cataccggat ttaaacgctt
      361 tatcggcgtg tgtaatattc cgatcggcat gaagatgttt attcacgatg ttctgatgct
      421 gaaagacagc gatgatttat ctatcgattt gtttggcctc aaccatatgg tgttcattaa
      481 ggatgtgctg gtaaatggca agtcgcgctt tgccgaattg cttgatggtg tggcatccgg
      541 gcagttaaaa gtatctggcg ttaaaaatat tttcgatctg ccatttagcg aaggcttaat
      601 tcgttctctg aatctgttgc cgtgttctta tttgctttat tacttcaagc aaaaagagat
      661 gctggctatt gaaatgggcg aatactacaa aggcggcgca cgagcgcagg tagtacagaa
      721 agtcgagaaa caactttttg agctgtataa aaacccggag ttgaaagtta agccgaaaga
      781 actggaacag cgcggtgggg cttattactc tgatgcagcg tgcgaagtga tcaacgctat
      841 ctacaacgac aagcaagctg aacattacgt taatatcccg catcatgggc atattgataa
      901 tattccggca gactgggcgg tagaaatgac ctgtaagctg gggcgcgatg gcgcgacgcc
      961 acatccgcgc attacgcatt tcgatgataa agtgatgggg ctgattcaca ccattaaagg
     1021 cttcgagatt gctgccagca acgccgcact tagcggagaa tttaacgatg tgttactggc
     1081 gctaaacctt agtccgttgg tgcattccga tcgcgatgct gagctgctgg cacgcgagat
     1141 gattctggcg cacgagaaat ggctgccaaa ttttgccgac tgcatcgcag agcttaaaaa
     1201 agcacattaa ccgaggctga ttatggaacg cttactgatt gttaatgccg atgattttgg
     1261 cttaagcaaa ggtcagaact acggcattat cgaggcctgt cgcaatggga ttgtcacatc
     1321 gacgacggcg ctggtgaatg ggcaggctat tgaccatgcg gtgcagttga gtcgtgatga
     1381 accgagtctg gccataggga tgcactttgt ccttactatg ggcaagccac tgacagctat
     1441 gccggggtta acccgcgatg gtgtgctggg aaaatggatc tggcagttgg cagaagaaga
     1501 tgctttaccg ctggaagaaa ttactcagga gcttgccagt cagtatttgc gtttcattga
     1561 gctatttgga cgcaaaccta cgcatcttga tagccatcat catgtgcata tgttcccgca
     1621 gattttcccg attgttgcca ggtttgcggc tgaagagggg attgcgttgc gcatcgaccg
     1681 tcagccgcta tctaacgctg gtgatttacc ggctaatctg cgcagttcgc agggattcag
     1741 tagcgcgttt tatggtgaag agattagcga ggcactgttc ctgcaagtgc tggatgacgc
     1801 aagccatcgg ggtgatcctt cgctggaggt gatgtgtcat ccggcgttta tcgataatac
     1861 tatccgtcag agtgcttact gtttcccacg gttaacggag ctggaagtgc tgacatcagc
     1921 gtcgttgaaa tatgcaattg ctgagcgtgg ttatcggttg ggtagttatc ttgatgtgta
     1981 agtaaggtgg taaggtcact ccccagggga cgtgacctta tccatattta cgccggaatt
     2041 ttgtcaatct taggaatgcg tgaccacacg cggtgttctg ccatcagcgt taacagttca
     2101 tccataaaac taccgtcggt gctgtcagct tccacaatcc cttcttcacc ctggtcagct
     2161 accttgattg ttgctttaaa cttgcgcgcg tctcccgcca gcgcaatcgg tttaaggtgt
     2221 ttgtaggctt ccatcagata gtagttggca tcgccgttgt cagcgatatc cgcgatattg
     2281 ccgcaaggga caatgaccgc atcgaccgtc agcgaaggtg caccggcaaa ggtagcggct
     2341 ataggcaaca ccgtaccgtc atccgcagtc acttcaccca ttcgggagta gagcagtttg
     2401 gcatgaacgc ctttggcctt catggccttg agaatggcca gaaggtctgc cgatctcact
     2461 tcatcattaa gtaaaatcgc taccacgcga cctttcacat caccgtcagg aatggcgtac
     2521 aaacttaagg atggatcctt tttcagaccg ttgacgtccg gaggtggggt gatattcagc
     2581 tggtcgtcag tcagttcgat accgagattt ttcgccaccg cctgggccag agtgagatca
     2641 atatgcgcca gctggtcaac aacgcgctca cgaatatacg gacgaacgac tttgcttaac
     2701 tcaaaactga aaccatcgac aatatggcgc tgctcgaatg gcgtctgact tagccagaac
     2761 agacgcggat gggaataata ttcgccaaac gatgggctgc gctcgcgaac tttattgcct
     2821 tccacgcgct cttggtatga ttcaaaaccg ccgcgtttcg gccccggtgg tgtttcgcgc
     2881 ggccagttat cgttgatcga gttcggttca taattcgccg ggttagtgtc gatccccata
     2941 cgatgcatgc cgtcacgctg gaaattatgg taagggcagg tcgggcggtt aatcggaatc
     3001 tcatggaaat tcggcccacc aagacgactg atttgtgtat cggtatagga gaacaaacgt
     3061 ccctgcaaca ggggatcgtt ggtgaagtcc agaccaggaa caatatgacc tggatggaat
     3121 gctgcttgtt cgttttcggc aaagaagtta tccggattgc gattgagcac cattttgccg
     3181 acacgctgaa cgggcaccag ttcttccggg atcagtttgg ttggatcgag aagatcgaag
     3241 tcgaacttga attcatcttc ttcaggaatc aactggaagc ccagttcgta ttccggaaaa
     3301 tcgcctgctt caatggcttc ccacaactcg cggcggtgga agtccgggtc acgtccggtg
     3361 agtttttgtg cttcatccca aacgagtgag gctttacctg ccagtggttt ccagtggaaa
     3421 cgtacaaacg ttgccttccc ttcggcatta atcaggcgga aggtgtgaat accgaagcct
     3481 tccatggtgc ggtaactgcg ggggatgccg cgatccgaca tcgcccacat cacgttgtgc
     3541 agagtttcag gttgcagaga aacataatcc cagaaagtat cgtgggcact ttgcccttgt
     3601 ggaattgccc agtgcggttc tggttttacc gcatgaacaa aatcggggaa tttatgcgca
     3661 tcctggataa agaagattgg cgtgttattg ccaacgaggt caaaaatacc ctcttcagta
     3721 tagaacttgg tggcaaaacc acggatatca cgcacggtat cggcagagcc agcgccaccc
     3781 tgaacggtag agaacctaac aaatactggg gtgattttgt tcggatctga gaggaaatcc
     3841 gctttggtga tatcgcttaa gcttttatat ggctggaaat aaccgtgagc tgctgagccg
     3901 cgtgcatgaa caatacgttc cgggatgcgc tcatggtcaa agtgggtgat tttctcgcgc
     3961 agaataaaat cttccagcag cgttggacca cggttaccgg cacgcagtga gttttgatca
     4021 tcggcgatgc gcacgccctg attagtggtc agcgcataat tttcactgcc tttgcgtacg
     4081 tcttccagag aattaagttt ttcgttacgt gtatcagggg ctttcaggct cccaggggcg
     4141 gtaggttgtg cacctggcgg tgttggttca gccgctggac gatgagatcc gtcctcaggt
     4201 gccagtgagt ccatccccgg tttcgcttcg ctggaatcgt gtagtggtga ctggtgctga
     4261 tgtgggttct tttcgttatg ttgcgacatt gaactcgtct ccttaattta ttactgaaag
     4321 ggccgctgtt ttggtcaaaa aactctctaa ctatagacca ccagccagat cccgcttcgg
     4381 agactattct ggaaacggtg ttatgaaata cacgcaaagc gggacgtaca ggggctaaat
     4441 cggctaaact acagtttttc taatttttaa tatgcgttta gtgaagccag taatgaagaa
     4501 accgctccgc cagcaaaacc gccagattat tagctatgtc ccacgcacgg aacccgcgcc
     4561 gccagaacat gcgataaaga tggattcgtt tcgtgatgtc tggatgctgc gtggcaaata
     4621 tgttgcgttt gtactgatgg gagagtcatt tctgcgctca ccggcgttta ctgtgcctga
     4681 atctgcccaa cgttgggcaa atcagatccg ccaggaaggg gaagtgactg agtaaggatt
     4741 acatacctcc cggttttgcc gggagttaca ggacctgaaa ttctgcattt cgttgctaca
     4801 ttcattcaaa ccacctgtaa caaaaagctg cctttgacga gctattgcta taaataagag
     4861 atcttttttc tttaattatt tatttatttt aaaatggtta agttttgcta atcatgatga
     4921 gttatttttg atagttcatc ctgtggcgtc gctcttaaac ttatattgta aatgacttag
     4981 atttaaataa gataataatt ttgttaacaa tatgaggcgg gagatgtttt ctacaaactt
     5041 aatacagagt aattatggtg atttaaatat taaaagcctg gctttcgact cttttaaaga
     5101 aaggcttgag tctaccatgg cggcactcac tttctttata tcaacgggtc aatgtgactg
     5161 cgatgaaata gcagagtcga acttcaatcg tatgattgcc tatatgtcta acattaacta
     5221 tgatgcctca aagctaggag cgccagcact tagttttgat acgtatctgc aggacaatgt
     5281 taaatataga gtgataatta ataatcttta tggcagtgag tttcggataa gagatattaa
     5341 tgagaatttt gttgggatgg attttacctg tgaattccga cccgaaaaaa tgacgaattt
     5401 gatcaatata aaaaataggt tggttattca ttatttaagg actatatact atgaaaaaaa
     5461 ttatatacat cctgccggga gcatttttgc tgcgattcaa aaaaaatgag agtctcctaa
     5521 agtttccttc gataatatca atgcttaata tcaatcttct ttttaatcca ctaaatcttc
     5581 cgggaatggg aagtggtgtg cttgaagata ttatgagtat tccagatagt tcacttagaa
     5641 aacgtctcgg gtatgaggtt ttaagcttct cactacaggc acattcattg agccaggaat
     5701 gtattgataa gttggacatt tttttcgctg acgat
//
LOCUS       DAJTUP010000176         5714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTUP010000176 DAJTUP010000000
VERSION     DAJTUP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.179"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="OO468_003831"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="OO468_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR6260764.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="OO468_003832"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="OO468_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR6260765.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5714)
                     /gene="mdtF"
                     /locus_tag="OO468_003833"
                     /pseudo
     CDS             complement(2659..5714)
                     /gene="mdtF"
                     /locus_tag="OO468_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
BASE COUNT         1505 a         1340 c         1349 g         1520 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attaccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aagggaattt ttacggcact aaccgttctg cggaaggaat aagattatag
     1201 agttttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt acaggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagtttttgc gctaagaaac
     1501 agtctgtcat catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcaa aaaatatcgc tgcataacga gagtatcctg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctgtta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcgga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt ttaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ttgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgccta ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagta ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgtggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagccgca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgca ccgctcataa aggccatcgg cataaacact gcagaaagaa caacggcaat
     4441 cccgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggcggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agtcaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaattgtcg ggatgatggt
     4681 ggcacggaaa ttctgcaaaa acagataaat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgttttg aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggc agccagtttg atggcgatcc cggcaacagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgcc ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccaga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca ggttatattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcaggatatt actgctcgac ttatcgacgc taatcccctg
     5401 ttgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg ccccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cccggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agca
//
LOCUS       DAJTUP010000177         5629 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTUP010000177 DAJTUP010000000
VERSION     DAJTUP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.180"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            17..355
                     /gene="spa13"
                     /locus_tag="OO468_003834"
                     /gene_synonym="spaM"
     CDS             17..355
                     /gene="spa13"
                     /locus_tag="OO468_003834"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR6260766.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            342..1220
                     /gene="spa32"
                     /locus_tag="OO468_003835"
     CDS             342..1220
                     /gene="spa32"
                     /locus_tag="OO468_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR6260767.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            1214..2095
                     /gene="spa33"
                     /locus_tag="OO468_003836"
                     /gene_synonym="spaO"
     CDS             1214..2095
                     /gene="spa33"
                     /locus_tag="OO468_003836"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR6260768.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            2100..2750
                     /gene="spa24"
                     /locus_tag="OO468_003837"
                     /gene_synonym="spaP"
     CDS             2100..2750
                     /gene="spa24"
                     /locus_tag="OO468_003837"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR6260769.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            2765..3025
                     /gene="spaQ"
                     /locus_tag="OO468_003838"
     CDS             2765..3025
                     /gene="spaQ"
                     /locus_tag="OO468_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR6260770.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            3025..3795
                     /gene="spa29"
                     /locus_tag="OO468_003839"
                     /gene_synonym="spaR"
     CDS             3025..3795
                     /gene="spa29"
                     /locus_tag="OO468_003839"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR6260771.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            3802..4830
                     /gene="spa40"
                     /locus_tag="OO468_003840"
                     /gene_synonym="spaS"
     CDS             3802..4830
                     /gene="spa40"
                     /locus_tag="OO468_003840"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR6260772.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            4843..5085
                     /locus_tag="OO468_003841"
     CDS             4843..5085
                     /locus_tag="OO468_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260773.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT         1794 a          794 c         1035 g         2006 t
ORIGIN      
        1 ctctttgtat cgagatgtgg aggcattaga taaaaggatt atatacttcc tgcaactgga
       61 gaatgactta gaacctgtag gcgctcagag tgttagtcag cttttcaata caagacgtaa
      121 gatcgctata gtaaaaaagc atattatcca atatcagtct gaacgaattc ttctaaaggg
      181 aagaattgaa gaaattcaaa aagatattga tgaggcaaat gcttctaaaa gaaaactgct
      241 tcataaagag agtaaaatat gtaaacggat tggcttgatt aaaagaaata actttgccaa
      301 acagttgata cttgatgagt tatcacaaga ggatatgaag tatggcatta gataatataa
      361 acctaaattt tagtagtgac aaacagatag aaaaatgtga gaaactatct tcaatagata
      421 atattgatag tctcgttttg aagaagaaga ggaaggttga aattcctgag tactctttaa
      481 ttgcatctaa ctattttact attgataagc actttgaaca taagcatgat aaaggagaaa
      541 tttatagtgg cattaaaaat gcgttcgaac ttagaaacga acgagcgaca tattctgata
      601 ttccagaatc aatggccatt aaagaaaata ttttgatacc agatcaagat atcaaagcaa
      661 gggaaaaaat aaatatcggc gatatgaggg ggatcttttc atataataag agtggaaatg
      721 cagacaagaa cttcgaaaga agtcatactt cttctgtaaa ccctgataat ctgctagaat
      781 ctgataatag aaatggtcaa attggtttaa aaaatcatag cttgtctatt gataagaata
      841 ttgctgacat catttcttta ctaaatggaa gtgttgctaa atcatttgag ctgcctgtaa
      901 tgaataaaaa tactgcagac ataaccccat ccatgtcatt gcaagaaaaa tcaatagttg
      961 aaaatgataa aaatgttttt caaaaaaata gtgaaatgac ttaccacttt aaacagtggg
     1021 gggctggaca ttctgttagt atttcagtgg agtctggttc ttttgttcta aaaccgtcag
     1081 atcaatttgt aggaaataaa cttgacttaa ttttgaaaca agatgctgag ggtaattaca
     1141 gatttgatag cagtcaacat aataagggga ataaaaataa tagtacagga tataatgaac
     1201 agagtgaaga agaatgctaa gaattaaaca ttttgacgct aacgaaaaac tacagatttt
     1261 atatgcaaag caactctgcg agcgtttttc gattcagaca ttcaaaaata aatttacagg
     1321 cagtgaaagt ttagtcactc ttacttccgt gtgtggggat tgggtaattc gtattgatac
     1381 attatctttt ttgaaaaaaa aatacgaggt attttcagga ttttctacac aagaatcttt
     1441 actgcattta tcaaaatgtg tctttataga gtcgtcatct gtattttcga ttccagaact
     1501 gtctgataag attactttcc ggatcacgaa tgaaatccag tatgcaacta ctgggagtca
     1561 tttatgctgt ttttcctctt ctttaggtat tatttatttt gacaagatgc cggtattacg
     1621 taatcaagtt tctcttgact cattgcatca tcttttagag ttttgcttag gttcatctaa
     1681 tgtaaggctg gctactttaa aaagaattcg cactggtgat ataatcatag ttcagaaact
     1741 ttataattta ttattgtgta atcaagttat tattggggat tatattgtga atgataataa
     1801 tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca gaacacacag aagtttcttt
     1861 ggcattattc aattatgatg atatcaatgt aaaagtggac tttattcttt tagaaaaaaa
     1921 tatgacaatc aatgaactaa aaatgtatgt agaaaacgaa ttattcaagt ttcccgatga
     1981 catagttaaa catgtaaata ttaaagtaaa tggttctttg gttgggcatg gggaacttgt
     2041 ttctattgag gatggttatg gtatcgagat tagttcttgg atggtaaagg agtaatctca
     2101 tgctgagtga catgtccctc atcgctacac tttctttttt tacgctatta ccatttttag
     2161 ttgctgctgg tacctgttat ataaagtttt ctattgtttt tgtaatggta cgaaatgcac
     2221 ttggtttgca gcaggttcca tcaaatatga ctctcaatgg tattgcattg attatggctt
     2281 tgttcgtgat gaaacccatt attgaagctg gttatgaaaa ttatctaaat ggccctcaaa
     2341 aatttgatac aatatctgat attgtccgtt tttctgattc tggcttgatg gaatataagc
     2401 aatacttgaa aaagcataca gatcttgagt tagcacgttt tttccaaagg tctgaagaag
     2461 aaaatgctga tttaaagagt gcagagaata atgattattc attgttttcc ctcttgcctg
     2521 catatgcgct aagtgaaatc aaagatgcgt tcaaaatagg tttttattta tatttgccat
     2581 ttgttgttgt tgatcttgtt atttcaagca ttttacttgc acttggtatg atgatgatga
     2641 gtccaatcac aatatcagta ccaataaaac ttgtattatt tgttgcgctt gatgggtggg
     2701 gtatcctgtc taaggccttg attgaacaat atatcaatat tcctgcttag atgatggagg
     2761 taatgtgtct gatatagttt atatgggtaa taaggctctt tatttaatcc ttatcttttc
     2821 cttatggcca gtaggtatag ctacggttat tggattatct attggtttgt tacagacagt
     2881 gactcaactc caagagcaga cacttccttt tggtataaag cttataggtg tctcaatatc
     2941 tttgctactt ctttctggat ggtatggtga ggttttattg tctttttgtc atgaaataat
     3001 gtttttaatt aagagtgggg tttgatggac atttcaagct ggttcgaaag tattcatgtg
     3061 tttttaatac tcctgaacgg cgtttttttt agattggctc cattattttt ctttcttcca
     3121 tttttaaata acggtataat ttctccatct attagaatac ctgtgatttt tcttgttgca
     3181 tcaggattaa ttacttctgg taaggtagac ataggttctt ctgtttttga acatgtttat
     3241 ttccttatgt tcaaggaaat aattgttggc ctccttctct ctttttgctt gtctcttccc
     3301 ttttggatat ttcatgctgt tggtagcatt attgacaacc agcgtggggc aacgcttagt
     3361 agttcaattg atcctgccaa tggtgttgat acgtctgagt tggcaaaatt tttcaatctt
     3421 ttttctgcag ttgtatttct atacagtggt ggtatggtct ttattttaga atccatacaa
     3481 ttgtcttata atatatgccc gttattttct caatgttctt tccgtgtctc aaatatctta
     3541 acatttctga ctttattggc aagtcaggct gttattttag ccagtcctgt tatgatagta
     3601 ttgttactat cagaagtatt acttggtgta ttatcgagat ttgctccgca gatgaatgct
     3661 ttttccgtat cattaactat taaaagttta cttgcaatat ttattatttt catctgttct
     3721 tctactattt acttttctaa agttcaattt ttcctcggtg aacataaatt tttcacaaat
     3781 ctatttgtta gataaaatat tatggcaaat aaaacagaaa agccgacacc taaaaaacta
     3841 aaggatgccg caaaaaaagg acagtcattt aaatttaagg atttaacgac tgttgttatt
     3901 attctggtag ggacatttac tataatatca ttcttttcct taagtgatgt aatgctctta
     3961 tacagatatg taataattaa tgacttcgaa attaatgagg gtaaatactt ttttgcagtg
     4021 gttattgtct tttttaagat aattggcttc ccactttttt tctgtgttct ttcggctgtg
     4081 ttgccaacat tggttcaaac aaagtttgtt cttgcgacta aagctatcaa gattgatttt
     4141 tcagtattaa accctgttaa agggttaaaa aaaatattta gtataaagac aataaaagaa
     4201 tttttcaaaa gcattctgct tcttattatt ctagcattaa caacctattt cttttggatt
     4261 aatgaccgaa aaataatttt ttctcaggtg ttttctagtg ttgatggctt atatcttatt
     4321 tgggggaggc tgtttaagga tataatatta tttttcttgg cattttctat tcttgttatt
     4381 attcttgact ttgtgattga gttcatttta tacatgaaag atatgatgat ggataaacag
     4441 gagataaaaa gagaatatat agagcaagag ggacactttg agacaaagtc gagaaggcgt
     4501 gagttgcata tcgagattct ttcagagcag actaaatctg atatacgtaa ttcaaaatta
     4561 gtggtaatga acccgactca tattgcaatt ggtatttatt ttaatccaga aatagcgcct
     4621 gcacctttta tttctctcat tgaaactaac cagtgtgcct tggctgtcag aaaatatgca
     4681 aatgaagttg gtataccgac tgtgcgtgat gtgaaattag ctagaaaact atataaaaca
     4741 catacaaaat atagttttgt tgattttgaa cacttggatg aagtcctacg tcttattgtt
     4801 tggcttgagc aggttgaaaa cactcattaa taagtaaagg agatgtgtta tatgggagtt
     4861 aatttctgta ataaaatagg tattgatcag tctgaatttg aaatagaatc ttccatcatt
     4921 aactccattg ctaatgaggt attgaaccca atatctttcc ttagcaataa ggatataata
     4981 aatgttttac tcagaaagat ttcatctgaa tgtgacctgg ttagaaaaga catttatcgt
     5041 tgtgctctgg agttggtcgt tgaaaaaaca cctgatgatt tataatcatt cataataact
     5101 cttttgctga tataaatatt tttgttgaag atatttatgt agtcacagga tcaattgtag
     5161 aaaattgtta tgtttttgtt aggaactgca ctaaataata tatgataaga cagcaaaaac
     5221 gactgaccat aatccttctt ttattaggag ttgataagag agactattca tcctgtaatg
     5281 ttaaaactct gttatatagt attcgcgatt atgcaaagtc tgtaaatgaa catgagatac
     5341 taaccgagag taatcgttta ttatctcatt gcatttcaga tagtaacgga gctttcttta
     5401 aaagtagtaa atacgttccc cttaagtact tacgtaaaag gagaatagca cgaaagatac
     5461 caaatgatta gcactttgct agtaagcttg ctggtaaact atattgaccg ttgggtttgt
     5521 tttgagactg taatttaaat tgtgtaattg cctgtttttg atatgtttac tccaacaacg
     5581 gagacaggta aattatggac gaaaagaagc ttaaagccct tgcggctga
//
LOCUS       DAJTUP010000178         5617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTUP010000178 DAJTUP010000000
VERSION     DAJTUP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.181"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..401
                     /locus_tag="OO468_003842"
     CDS             <1..401
                     /locus_tag="OO468_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6260774.1"
                     /translation="QRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYY
                     SDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGIDPEA
                     YLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            433..1047
                     /gene="istB"
                     /locus_tag="OO468_003843"
                     /pseudo
     CDS             433..1047
                     /gene="istB"
                     /locus_tag="OO468_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1216..5310)
                     /gene="sepA"
                     /locus_tag="OO468_003844"
     CDS             complement(1216..5310)
                     /gene="sepA"
                     /locus_tag="OO468_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR6260775.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1384 a         1407 c         1337 g         1489 t
ORIGIN      
        1 agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag aagaatggta
       61 cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat cagtgggatg
      121 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcagaaagag
      181 cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc gatcatggag
      241 gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac ggtatcgatc
      301 cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc aaccgagttg
      361 acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata cggccttccg
      421 ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta taaaaaggag
      481 gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt gccggaactg
      541 aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc tggagaaaac
      601 gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc gatcgtggat
      661 ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct gttgcaggaa
      721 ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact ggatcgctac
      781 cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga aacgggtgtg
      841 ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac cagtaaccat
      901 ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc ggcagcagac
      961 cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag gaaaaagaca
     1021 gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag tatagttggc
     1081 gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga gaaaaggcat
     1141 ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg ccgaatggca
     1201 gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat tatccacatt
     1261 atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat ccttaatttc
     1321 cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga aacgtttctc
     1381 tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact gataacccag
     1441 cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca cgtcaacacc
     1501 agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca ttccccggtc
     1561 ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct gtggctccac
     1621 ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat accaggaatg
     1681 agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat tatcatgatg
     1741 gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag aggcatacag
     1801 accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat tgctgtccgt
     1861 ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct ttctgtcgac
     1921 accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac cggtaccatt
     1981 cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac ccatacgttt
     2041 gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa agtcttttgc
     2101 ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc attttgtggc
     2161 atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa tctgctgcgt
     2221 accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg tcacgctcat
     2281 ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc ctgacagttt
     2341 gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga acgtggtatt
     2401 atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc ccagttcaat
     2461 tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt accacagagc
     2521 attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa cagcgttata
     2581 tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag catcactgtc
     2641 tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca gatagcccat
     2701 atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg tagaaattac
     2761 gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca gagaagcatt
     2821 ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa gttttgctcc
     2881 ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa cagcattctg
     2941 agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc tctccatgat
     3001 ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct ggtcaccaac
     3061 tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc cgtcactcct
     3121 gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg cgttaatatc
     3181 tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt tcagtttttc
     3241 cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg ttccagcatg
     3301 aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg aaccttctgg
     3361 caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg gaatattcac
     3421 atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg cctcaatttt
     3481 ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct gccagacact
     3541 gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga tgaaatattg
     3601 cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa aaatactgac
     3661 agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga gtgttaccgt
     3721 tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct gaagacgggt
     3781 aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc acgaaatatt
     3841 atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc tggcaatacc
     3901 aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga gaataacggt
     3961 accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg tcagggtacc
     4021 ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca gaacgtgggt
     4081 tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg cggacaccct
     4141 gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct gcagaacgac
     4201 ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat ttcctttctt
     4261 tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat tagccgcatc
     4321 atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt caaaatcatt
     4381 ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc catagttagt
     4441 tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat aagcaaacag
     4501 gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg cgggattaaa
     4561 aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca gcgcgctgac
     4621 ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac catttttgtc
     4681 tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc cgccggcctg
     4741 ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct ctgtcaccag
     4801 tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac caaccgcagt
     4861 ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat gtttggcggt
     4921 aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac cggtagtaat
     4981 agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca gattgccctg
     5041 cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat tctcggcaaa
     5101 gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag ctggccatgc
     5161 cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa gtctgcgatg
     5221 gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt tggttatatg
     5281 gcaatactta agataatata ttttgttcat atagaaacat caacctaatt ttgacaaaga
     5341 ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa ttattaagca
     5401 tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa caaccaaaaa
     5461 aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg atactcttct
     5521 cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga taaggggcag
     5581 tttggatatc gagaaccacg gcagcctgac ggctgca
//
LOCUS       DAJTUP010000179         5271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTUP010000179 DAJTUP010000000
VERSION     DAJTUP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.182"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..371
                     /gene="mddA"
                     /locus_tag="OO468_003845"
     CDS             <1..371
                     /gene="mddA"
                     /locus_tag="OO468_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR6260776.1"
                     /translation="YPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGK
                     GLGRKLLSRLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHW
                     LDLTFMQLQLDERTEPDAIG"
     gene            368..817
                     /locus_tag="OO468_003846"
     CDS             368..817
                     /locus_tag="OO468_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR6260777.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(818..1051)
                     /locus_tag="OO468_003847"
     CDS             complement(818..1051)
                     /locus_tag="OO468_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR6260778.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1137..1310)
                     /gene="ortT"
                     /locus_tag="OO468_003848"
     CDS             complement(1137..1310)
                     /gene="ortT"
                     /locus_tag="OO468_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR6260779.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1505..1600
                     /gene="yncL"
                     /locus_tag="OO468_003849"
     CDS             1505..1600
                     /gene="yncL"
                     /locus_tag="OO468_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR6260780.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2002..2811)
                     /locus_tag="OO468_003850"
     CDS             complement(2002..2811)
                     /locus_tag="OO468_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR6260781.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3021..4445)
                     /gene="patD"
                     /locus_tag="OO468_003851"
     CDS             complement(3021..4445)
                     /gene="patD"
                     /locus_tag="OO468_003851"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR6260782.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4467..5261)
                     /locus_tag="OO468_003852"
     CDS             complement(4467..5261)
                     /locus_tag="OO468_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR6260783.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
BASE COUNT         1381 a         1336 c         1140 g         1414 t
ORIGIN      
        1 gttatccggt gctggtaagt gaagaagatg gcgtagttac gggatatgcc tcgtttggcg
       61 actggcgtag tttcgatggt tttcgccata ccgtggaaca ttcggtttat gtccatcccg
      121 atcatcaggg caaaggtctg gggcgtaaat tgttaagtcg attgattgat gaagcgcggg
      181 attgcgggaa gcatgtcatg gtcgccggga tcgaatcgca aaatcaggct tcgctgcatc
      241 tgcaccagtc gctgggattt gtcgtcaccg cacaaatgcc gcaggtaggc actaaatttg
      301 gtcattggct ggatctgaca tttatgcagt tgcaactcga cgagcgcact gaaccggacg
      361 cgattggatg aatcagtcgc tcacccttgc ttttttgatt gccgccggaa ttggtctggt
      421 agtccagaac acgctgatgg cgcgcatcac ccagacctcc tctaccattc tcatcgccat
      481 gttgctgaac tcactggtgg ggattgtgct ctttgtttct attttatggt ttaaacaagg
      541 catggcgggg tttggcgaac tggtgtccag cgtgcgctgg tggacactta tacctggctt
      601 gctgggttcg ttttttgtct tcgccagtat cagcggatat cagaatgtgg gagcggcgac
      661 aactattgcg gtactggtcg ccagccagct gattggcggt ctggtgctgg atatcttccg
      721 cagtcatggc gtaccactac gggcgttgtt cggcccaata tgtggcgcaa ttttacttgt
      781 ggttggcgcc tggttggtgg ccagacgctc attttaatca gaggatggtg ccgcctttgg
      841 ttaactgttc gtggcgcgct tccatatctt ctttatgctt gcgaccgtga tgagcaatag
      901 cggtgcgcaa acgctgctgc tgggtgtagc gatcttcccg acttaactcc gcgtcatcgc
      961 tcagggcgat taataattcg ttcatatggg caatgacgct ctcttcaatc gcggcatcca
     1021 cgcgcgcgat gacttcatcc agatgcgaca tactttctcc ttagccactt gtgaaattta
     1081 tcgattaatc caaatgatat aagtctgcgt ggtgccgtca agccagtgag taagatttaa
     1141 aacagtgaaa acaacagcgc caccgggaaa ctcatcggcc atgtggcccc caccagaaag
     1201 gcgcttaaga aacggatgcg tttcttatcg tgagaaagaa accaggtgat aagaaatgcg
     1261 attgctgcca taaccgcata aaaaacaagc atatgttgat agagaaacat gtacgggcgt
     1321 ccagccatat gaataaaacg cgcgcaaata tgcgccattt tttgacgtgc atcaagtttt
     1381 ttcaccagtt tgagaaaaaa tcatcaaaca gcaacttgcc cttatccatc ttcagaatga
     1441 gtattattgc ggattttctt aacccgtact atacacatag gggcaatgat aaaaggtggc
     1501 aaaaatgaat gtttccagta gaactgtagt actgataaat gtctttgctg ctgttggttt
     1561 gtttactctt atctctatga gatttggctg gtttatttga tgtagtgatt ttgcacgcag
     1621 cacgtactgt cagagaaatc aactctctga cagtacgcaa gatcttctca tactctccaa
     1681 gtttgcaaca ttatccttaa tatttacttc tttttgctgg catctacatt gaaatgagta
     1741 gaaaacatcc ccctactgtc gtctatacaa acagcttact gattcaaacg gaatcttgtt
     1801 ttcacatttt tctatcacga atactttata cgcgcgggag tctataaatg ctttatactt
     1861 tcaaggaata acgtaaatat aaaaagatgg cgtaaagtat atgcatggca ggatgtttgc
     1921 aacctccttg atgacgctgc cccattgaaa ttgcaaaccc cgccctggtt atcttgccca
     1981 tttatcatgc aaaacagata attataggcc tctgaatacc tcagaaagat tgaatttttt
     2041 atagtccctc tcttgaatat ttccatttga tatcaatcga ttcatctctt caacaaaata
     2101 atttatatca tcacacttat tattatccgg aagatctgag tagttataat tccaccattt
     2161 tattctcaac agttcatcaa taacatttga atcaaaccta aatctgatta tttttgcagg
     2221 aactcctgca actatggcat atgggggcac atctttggta acaacggaat tggcagcaat
     2281 aacagcacca tcacctattg cgatgccacc tttcaataca acatcatttc ctatccagac
     2341 atcatttcct ataatcggat tttcaggttt ggtaatgaat ggtttcaatg tccagctacg
     2401 ttcaaattca ctcatagcaa acttatcaaa ttcaccgtta taagtcatca tgtgagtagt
     2461 aaaacgattc aaaggatgat ttccacccat tatttttaca tttgatgcaa tactacaaaa
     2521 tcgacctata cgtatattcc ctgaaaaatg gcaggtcgga aatgaaaaag cgccgacact
     2581 ggagaaattt ttcacaggca ttgttgaata ttcttcagca ataacattat cttcaattgt
     2641 tatcttgtca ccttttgaga aaagaaataa attcctttct ttatacgact tcttcccctt
     2701 atgatttaaa aagattttat tatccataca aaactcaaag tgttttttcg tccatctgaa
     2761 ggtaagggtt ttatttttat cttccgtgaa tttgtacaat tgaactttca ttgttaatac
     2821 ttctacttat aaagtattag tttaataacg atactctaaa gccttatgct aatcaactgt
     2881 gacaggatga aaaatcgcca tcaaacattt agcctaattt ttacgccagt tattaatgta
     2941 atcattctaa tacttaaccg ccattaaact cagaacctaa aacgatagtg gccgtcctat
     3001 aacagccact aacatctaac ttaatgttta accatgacgt ggcggacgac ggtgtaatcc
     3061 tccagcccat aaagtgacat atccttgccg taaccagaaa gtttctgccc accgtgcggc
     3121 atttcactta ccagcatgaa atgggtattg acccaggtac aaccatattg cagccgtgcg
     3181 ctgacgcgat gcgccctgcc cacatctttc gtccataccg aagatgcaag tccgtactgg
     3241 ctgtcattcg cccagttcac cacctgttct tcgttgtcga agggcgtaac actcactact
     3301 ggaccaaata cctctttttg cacgatggca tcgtcctgta atgcgccagc cagcagcgtc
     3361 ggcgcatagt aatagccatt acccttgcgc ttttcaccgc cagtgatcac tttgatgtgc
     3421 cctgtcgctt tcgcctcttc tactgccttg ctgacgcgtt cgagatgcgc cagcgagctt
     3481 aaaggtccaa gctccgtaga ctcgtcatcc ggcgcaccag attttaacgt tgccaccgca
     3541 gcacccagtt tttccaccag cgtatcgtaa atgccttttt gcgcgtagat ccgacaagcc
     3601 gcagtacaat cctgtccagc attgtaatag ccaaatgtac gtacaccttc gaccactgct
     3661 tcaatatccg catcatcaaa aacaatcact ggcgctttgc caccaagttc catatgagta
     3721 cgcttaatgg acgacgcggt atggctgatg atgtgctcgc cggtggcgat agagcccgtc
     3781 agcgacacca tccgcacttt gggatgaccg gtcagcggat cacccaccgt tttgcctctg
     3841 ccaaacagta tgttaatcac gcctgccggg aagatatctt tcgccagctc tgccaacttc
     3901 aacgcggtca gcggggtaat ttctgatggt ttaagcacta cgcagttccc tgccgccagc
     3961 gccggagcaa gtttccacgc ggccatcatc agcggataat tccacggtgc gatagaagcc
     4021 acgaccccca acggatcgcg acggatcatc gaagtatgac cttcaagata ttcacctgcc
     4081 gccagaccat tcagacagcg cgccgcaccc gcgaaaaagc gaaaaacatc gacaatcgcc
     4141 gggatttcat cattgaacgc actatgcagc ggtttgccac aattacggga ttccagttcg
     4201 gcaaaaacct gaccattttc ttcgataaca tccgccagtt tcagcaggca ttccgcacgc
     4261 gctttcggtg tggtttgccc ccattcagca aatgctgcat ctgccgcacg cacagcagca
     4321 tcgacctgct ctgcggatac ctcggcaatc tccagtaaaa cgtcccccgt tgccggatta
     4381 tagacaggct gtttttcccc ttcgccgcta acccgttctc cgttaatcag taacttatgt
     4441 tgcatagctt attcctgtat cagtggttat tgaccattgt cgccttcgcg ggttagccac
     4501 caggccccca ggatcggcaa ggttgttacc aacataacca gcagtgccac cacgttggtt
     4561 accggtacat cacgcggtcg cccaagctga ttgagcaacc acaacggtaa cgttcgttca
     4621 tgacctgccg taaaggtcgt aacgatgatt tcatcgaacg acaaggcaaa cgccagcatt
     4681 cctcctgcca gtaacgccga actgagattc ggcaacacta cgtagcggaa ggtttgccag
     4741 ccattggccc caagatccat tgacgcctca accagactcc acgaggtgcg gcgaaaacgg
     4801 gcgatgacat tgttaaacac caccactacg caaaaagtcg catgaccgac cacgatggtg
     4861 aaaaatcccg gctccagatt gatggtttta aaggcggtta atagcgccag accagtgaca
     4921 atgcccggca gcgcaatggg cagcagcaat aacagcgaaa tggcgttttt gccgaaaaag
     4981 tctcgtctcc acagcgcggc agctgccagc gtccctaaca ccagcgcaat taatgtcgcc
     5041 agcgccgcca ctttaagtga cagcgtcacg gcatcaagaa tatcactacg ctgtgctgcc
     5101 acgctaaacc agcgcagcgt caggccctgc ggtggaaaac taaacgccgc atcttcagtg
     5161 ttaaacgcat aggcggcgat tatcaggatg ggaaaatgta ggaaaacaac gccgccccag
     5221 gccgccagtt tgaggaaaaa cggtgcgcgt tcagagtgca tcgaacgctc c
//
LOCUS       DAJTUP010000180         5230 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTUP010000180 DAJTUP010000000
VERSION     DAJTUP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.183"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(106..1815)
                     /locus_tag="OO468_003853"
     CDS             complement(106..1815)
                     /locus_tag="OO468_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR6260784.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2247..2966)
                     /locus_tag="OO468_003854"
     CDS             complement(2247..2966)
                     /locus_tag="OO468_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR6260785.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3091..3647)
                     /locus_tag="OO468_003855"
                     /pseudo
     CDS             complement(3091..3647)
                     /locus_tag="OO468_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3631..3889
                     /locus_tag="OO468_003856"
                     /pseudo
     CDS             3631..3889
                     /locus_tag="OO468_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3998..4576)
                     /locus_tag="OO468_003857"
     CDS             complement(3998..4576)
                     /locus_tag="OO468_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260786.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4997..5230)
                     /locus_tag="OO468_003858"
                     /pseudo
     CDS             complement(4997..5230)
                     /locus_tag="OO468_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT         1482 a         1113 c          908 g         1727 t
ORIGIN      
        1 ttgccaattg aggtggttgc ctgaaagttg gcagttaccc ggtcactaca ccttgttgga
       61 gtgaacatat caaaaacagg taattacaca atttaaatta cagtctcata aaaaatgatt
      121 aaatctattt attatttcaa taaacgtttt tttcatcttc tttgaaaatt gttctgctat
      181 agtatccggt tccagattgt tctcttttat tattctacaa tactctaata ttgtttcttt
      241 gtctctattt actacagata caaatacacc aggaatacca tctttattag ttgcggacag
      301 catttcgacc aatcgtcttg tggtaaaact atagtttttt agaatattac aaaaggcacg
      361 tattgtttcg gtatggccat tataaaatgc cataaaaagt cctgatgtac cgttgggatt
      421 ttttatccat aataattcat ctatatattc ctggtgaggg gggagtattt ttagtatatc
      481 tccatacgcc ataatggcct ttgtgtgccc attttgaagt gccataaaca acccatgctt
      541 tactttcaat aattctttaa tttgttcact actaagcatt ttcttattaa gcagacttcc
      601 gaacatacga atactatctg catgtccatt ctgcaatgca aaaaataacc caggtaaatc
      661 atggctgtct ttcgcagcta atagatataa tctcttatcg gaagttaacg gggttttaaa
      721 gactatttcc ccataggctt ttattgtgtc tacatgtccg ttcataaaag acatgcataa
      781 acccggcaca ttgtttgccc ctaccgcagc aagcaatgta agtattgttt cttgactaag
      841 attgcacata tttaatatct taccatatac ttgaataatg tctgaatatc cgttttgaag
      901 tgctatataa aatgcaggag taccatcaga gcttctcgtg gccagaaggt tcaccagttt
      961 agattgagaa atttcaccat ttttgtattt ttccatcagt ataaataata cttctgtcag
     1021 attctttgtt atcgcatgat atataactct ggaattgatc aattcatctg gtaaaagttt
     1081 cactgtgttt gttagagggg aattatgcct atcttcaaat attacctgcc cgggttgctc
     1141 cagaccataa attttccaat aaaaatcatc aaaaaaatcc ttagcggata attgactcag
     1201 ttcgaaatgg ctatcacaag taaatacagt acgtttatga gtaacagttc gattaggatc
     1261 gaaaaactga acaacccatt tttcactttc ttgtacgcgc tttattttca accctaacgc
     1321 catttggtgg tttaatgtgc atgctgataa tgttctgaca aagtctccat tttttttcat
     1381 gttataaaaa aaattagtaa taactgttcc ccacttttta ttttccacaa aataaatata
     1441 agctggtgat tttgaaaaat cttccgtttt ttccaaacat acattttttg caatagcatc
     1501 aggggtgcta agaagctgat agtttacctt cccatttgtt tccataaaat tcatttccca
     1561 ataggaagct aggtgacggc aaactatttg tcttccatat atatcttttg ccactccatt
     1621 tatatttacc tccacatttc tcttttctga aaaataaggt gctgttttat tcttaaactg
     1681 aattagttca ctggagtttg agaccctcat aatagaactt gttattgttc tattatctct
     1741 gttaacagac atatctgttg ataacgaatt ttttgttgaa aatatactag atgaaaatgt
     1801 tttatttaac ggcatagaag acaaccatag agatgttata atactattag attgcagtta
     1861 ctaatagtaa aagcctcctt acaaggaggt agcttattca gaatgctcct ataagatagc
     1921 gagctcgtca acgatacaga tgatctgatt atattgtgca aaaggagaac aggaaaatag
     1981 gcactggaaa agatgaaggc ctgatgggag cattaactaa tgcaaaatcc gacaggttaa
     2041 tatcccgaaa agcaatcaaa cgactcaaac aggagatgca cgctgaaatg gtggacaact
     2101 gttcggattt cagatgagta gctcagcagt ttatgagccg aatgctctgt cacgggcagg
     2161 aatcaggctg catacgcagt gccaaatcta atcgtagtta ttctaaccag ttttcaagct
     2221 acaaggtggt gtagctggca tcttctctac tctatcatca aacgataaaa tggttcctct
     2281 cgtaacattt gctcttgcct ttcacttcca tctcgatcgc ttcgtagttc attacgatag
     2341 ctgacatatt tccagtcttc aggtctaaca tctgacgccg gatactcccc gggagcgatt
     2401 ttgcttctta atagattaga ctcgagacac attataaatt gtcgtatttt atgaagtaat
     2461 aaagcactat attgcgagca ttcctgatct gattttacat atagcgtaaa ctgagctcct
     2521 atccccacac gagattgctg agaaactcga ttcatatcag ttatcttcca tttatctata
     2581 ggactgtcct ctgaaaataa taaaccagac aaaatttgga atgccagagg aacttgctcc
     2641 ctagctatac taatatgaaa cttgtcccca acaaaatccc ccttactctg aggattttca
     2701 cgacatgcat gaataaacac atctccacta tggttcacaa tctggaatcc ttggtacatg
     2761 acagaaaaag attgacatat atcagagtaa gttggcacgt tcgatgctac tttgaaatag
     2821 tacgctgggt attgccgtat ttggttgtac aatatattga agttattttt caatctttca
     2881 ttcgcagaaa gcatctgcgg tatttcagac ctcacaacat tcaaagaatc taaatttagt
     2941 tttagacagg gcttttttat gggcatagaa aacgtcctct ataaaataga tatattatct
     3001 tatattgatt gtttatcatc ttttataaaa cgatacatta tatgtaatgt atgtaagcgt
     3061 aagggtcctc tccgaacatg gtgggaaggc ttagaacgcc atattcagtt tcccatagtg
     3121 gaacatcact cccagcttaa agcgctcccg gttgcggtag cccttggctt ttattctcag
     3181 cagcctgatc ttgctgttca gtgcttccgc atttccgttt gatacgccat ggcgcatggc
     3241 gttcaggata ccgtacaacc ttttccctat cgttttagcc acattcttca tcatggggac
     3301 gtcactgttg gccgcagcca tctctgccag tcgttgcgtc tttcctcgct ccatggtctg
     3361 ctccagatat cctttgccag ttctttcagc gcccagcaca ggctcgtcag cttcatctgc
     3421 gcacgcagcc atatcagctt ttcctgcctg gattcggtca tccatttatc gctgtgctgc
     3481 cacaggaagc gagttccctt tgcctgacga cgactttcaa caggaagatg cggatgttca
     3541 ttctggcggg ttttatccac cacctcaccc ggttgtttcg ccacatgaaa gcgatcgaag
     3601 gctattttct caaccgcatt gggtaagtgg atgcgcgctg ctcttagttt gctgagccag
     3661 tttagcattg ccttctacat gaccgatgcc tgagccggta taccgaactt tattgtcctc
     3721 aaccagtcat gttataagca agaaatgcac tcagcggata gagcgacata atctgaacct
     3781 gcggacacat cttaagcggc ttacccgcaa aaccatatgc ttctcaaagt ctgatgatat
     3841 gcactacaag atcatcggct ggtatctgac aatcaaccac caccactaaa tctgcgtcac
     3901 gacctgcaac tgagtctggt tgaacaggct gaggccctgc tgaagcaacg cgaacctctc
     3961 gactcttgtt ggattttacc aaatagtttt tctaaaatta aacacattta tataagttct
     4021 ttgctgaaca aaacgcagcc cgccatcctt tttcattcat aaattcagca atggcagata
     4081 aaactttttc atcaagcata taaaaaataa tgccagtaga ctcaatatgc ctcatacaaa
     4141 taaggccgca catatccgta atatatttgt caatgctgct accatttgca tggtaatatg
     4201 tcatgctatt atctgatgat tttacatatg cagtatgaca tatttttcct tcacgattaa
     4261 caaaactgac aaaaccactt tcaccaggag taatatataa agatgaattt aacgtgcgct
     4321 cactaatatt cattaattgt tgaaattcaa tggaatagtt ttcagttaat gtatctatta
     4381 tcttggcttt gtctttcttt gttatctcat tgtctttttc ggcaagaaaa attgccactt
     4441 tatgacatgc attcagctcg ttaattttac ctgataaaac tgcttttctt accacatcgt
     4501 tgaactcatc gatagtgtgt tttttattat atatatcatg aaaaatatag cctaaagact
     4561 taaacgagac tttcatttta tattctcctt aaatattaac aggatatata tatcacattt
     4621 tcctctatat tttcagaaac cagctatcat tttccccatt actggtacgt cgcttcagat
     4681 atttccgcag caagcgataa ccctgcgcac tatgaggatg ccattcatta taatgtttga
     4741 acaccttcgc gagactactt tactgctgtt aacacgtcag gttcgaacat aacactgatt
     4801 taatcatatt tcatcgtttt gacgaagttt tctgccattc cattttgcac cgccgtgtgt
     4861 ttgggggcaa ggccgaccat ctgtgtttca ttcgccagat ataagtgatg agatggcatt
     4921 ctccttcctt cacggcagaa gcattttgac cgtttagggg atgcggacga ttttcatgcc
     4981 tgctaagata ctttcgttac tgatgctgtc cgttttccac catttcatca tcactgattg
     5041 gcgttgtgtt tttggtcagc actccagctt tacgtttctc ttttaaccgg taactctctc
     5101 ctttgatatt cagcgtgtag cagccgatct aggatcgctg ttgccagcac gtgatctccg
     5161 aacatttctc cccagtctgc gacccctttg tttgacgtca gtatgatgct cgctttttca
     5221 tatcgacagt
//
LOCUS       DAJTUP010000181         5095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTUP010000181 DAJTUP010000000
VERSION     DAJTUP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.184"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1372)
                     /locus_tag="OO468_003859"
     CDS             complement(<1..1372)
                     /locus_tag="OO468_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR6260787.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGM"
     gene            complement(1388..2272)
                     /locus_tag="OO468_003860"
     CDS             complement(1388..2272)
                     /locus_tag="OO468_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR6260788.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2283..2888)
                     /locus_tag="OO468_003861"
     CDS             complement(2283..2888)
                     /locus_tag="OO468_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6260789.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3059..>5095)
                     /locus_tag="OO468_003862"
     CDS             complement(3059..>5095)
                     /locus_tag="OO468_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HCR6260790.1"
                     /translation="VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSIN
                     ASGLVFIDSYYDNPSTIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVK
                     KALMIINPDAYYWGLINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQE
                     LQMLDEGNVAAFEGRDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYM
                     PDNEILELTDQQLGYQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDI
                     VMTLAKRDWDMIIQELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLAN
                     DSSNNVVTSLAFKLAEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPD
                     NLQFNFVKRMEKKSPAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSM
                     KQKIWQRALSHRKNNPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWD
                     FLESALVSWDNLPAVVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPD
                     ALAQLDVLAKQPDRIWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNN
                     PRFPVDVLKARLKRDPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1489 a         1258 c         1052 g         1296 t
ORIGIN      
        1 acattccccg ttgggcaatc atctcttgcc cctgtgcaac atttaactca ctgtaatcac
       61 gtaacaaaat atcgcttacg gcaggctcgt ttgccagcgc atccagtaca tagcgtccat
      121 gccctgcggc aatgtcaacc acgcggacgg ctaaaccttt ggcgtggaga tcggcaacgg
      181 cttgtttaat caatatttgc aaatgggttt tgcgctggcg aataccgcgc catccaacac
      241 tgttcaggta gattttgtcg attaaacgcc cgaatgcgtt actgccttgc ggttgatttt
      301 gatagacata atccagggaa ctgcctgaat caaagccggt actcattccg agatgcaggc
      361 ccgcagagtg cgcaccgaac aatttcatcg cctttcgcat aaagcgatac gctaaatcaa
      421 ccggcgataa tggcacgggt ccaccagaaa ggagccgcca tcgatccgct gatggtcctg
      481 tgcggtcttc atgctgataa tcaaattttt gcgatttgtt agcatataag cggcagataa
      541 agctttgcat tttttcaaat gcctgcgccc tgttctcttc acccaatgtg tcgtgataaa
      601 agcctggcag cagatgcagc tctttcagag ggctacgtaa tctctgataa aaatcaatct
      661 gcggttggcg atgcaccaca tagtcatcac ctgatatcag aagttgcgtg gggagcgtaa
      721 tcgccgccgc atcgctaaca atacgttcag acgttttata gagatcgagc aagatattga
      781 cggcaatcgc ccgtgtgatc agcggatcat tattgaaact cgccacccgt tgccgatcgt
      841 gggtcaaata gcgccctttc acataggaat taataaaaaa tagaccacgc agacgatgcc
      901 ataacgccag cgcaggacgt gccagcggca catacaattt aaccttaaag gccggagaag
      961 ccagcaccag cccacgaatt gcaggtgcat aatcatgaac ccatgtggca acaagcactg
     1021 cgccgacgct ttgcgcgatc actaccacct cttccagtcc gacctggctg tcgctggcag
     1081 caaaacggac aaattcatcg acatcctgca ctgagcgcat aagagacggg ctataaccac
     1141 gcggccccga attttttcca tgcccccggg catcccaggc ataaaaagca gtatcaggca
     1201 tcgccagttc atcaacgaga tgttgtagac gaccagaatg ttcatgcccg cgatgaaata
     1261 agacgatgac ctttttcgcc cctggctgta aagtgggcca gtgccgataa tacaatgctg
     1321 tattatcact ggtggtaaaa aaatgttccc cagggatgcg tgaattttcc attttctcta
     1381 ttttccttca gtaacagcag tagcgaataa aataaaaaaa taccggagcg gtgaaaatta
     1441 atgagtcaat cctgtcaagg agtccgccat gtcctagtaa tagttttcca ctatctttaa
     1501 caccaatatc tcgtttgatg gctgacatca ctacatcgcc gcaaaaacca ctaataccaa
     1561 ttaacaaacc cgctaataat gcctgtaatg tatttagcgg tgtcagcaac ggtccaataa
     1621 tcagtgacgc aatcatggtg gtgattacgc cccccagcag accttccagc gtttttcccg
     1681 ggctgacttt ggggaccact tttcttctgc cgcaggattt cccccataaa tactgtgcaa
     1741 tatcgtttga ctcggttaac gccaaaagaa ataacaccag taacgccccg ccctggatat
     1801 ttatggttgg caacattaat agccaggcgg catgactcaa ggcaaaaacg gtcattatcc
     1861 atccccaaaa aatggcgctc acggtatgct gaaaaccgga ggggtcaccc acaaaaactc
     1921 gccaggtggc taatatcaga aacccagtca aaggtataaa taacagaaac agctcaaagc
     1981 aattaaaacc gataagcaaa tagttaagag gaataaccca atataaccaa cgcgaaaaat
     2041 gtacggatat aagcgtacaa tattctttca atgccagaaa acttatcagc gtaaaaaacg
     2101 tcaacgtcat ccagcgtggg cctgaaatca ccagtgaaaa acaaatgacg atgccccacc
     2161 aggtacgaat acgtaatgta acttcatccc cttttctctc aggtaaccgc catagcagaa
     2221 aacggtttat cagcttgcta aaattaacaa cgcgaaaagt gttgccagag atttttccag
     2281 cattatcttt cagcgctcat aagaagcaca ctccgacaac gattaatcgc agtacagata
     2341 agcagaattg aagcaatgct ccataaaaga ttattccatt gcatccactg tggataaatg
     2401 gcaacggcca gaccccacag accaaatatt aacgcgcgat cacttttgcc aaatggaccg
     2461 gcataactgc gaacaccgtt aatcgtctgc gcgagtaaac cgcaaaactc ggtcaatatg
     2521 gtgcaaaata acatgagtat cacgagtgat gcgttacttt ccggtaaaaa taaaaacggt
     2581 aaatagagcg caatatcgga aataacatcg ccagtctcat tcaaaatcgc ccccagacgt
     2641 gtttgctgat tgcactcacg cgccaacatg ccatccagcg cattgagcgc catacggata
     2701 aaaagcacga tgggcaatag caaaaagagg atgggttgtg ccaccaacac caacagcaat
     2761 ccggtaaaaa gagaaagcgc cagtgccgta agagtgatgt gattcgctgt aacgtggtgc
     2821 ttataaagcc aaaacatcgt cggccttaac agcgactgaa agagcggttt tatctggtag
     2881 agcgtcatta tcaatccttg ataattcggt ttgcgctatg gtgcatcaat tcccctgacg
     2941 gagatatcgc aacacgccgc ttcattaaaa attcagaata tatcaaggta ttgggggctc
     3001 actgtcagga atgattatcc ccaataaatc aagtagttaa aaattattac gctgttattc
     3061 aatacaactc atcaagtttc ctcatcgctt gttcccgtat tgcgcgcgtc ttaccgttaa
     3121 gcgccagctg gcggactaaa tccagttcag ggttaacaag ctctaacgcc agcaaaggat
     3181 cacgtttcag cctcgccttc aatacatcaa ctggaaagcg gggattattc atcgccacaa
     3241 tccaccactc gggatcaatt tctgccgcca gtgccgcaga gggggtgtgg cggttgctaa
     3301 gtgcgccgaa cttgaaaaat gtgagattgc ttttcgccag tgtactccac catatacgat
     3361 ccggttgttt tgccagtaca tccagttgtg ctaacgcatc ggggtcgagt gcataataaa
     3421 cacgtaatgc ttcatcaacc tgcgcacggg agctattttc ctgcctcaga aaaaacttcg
     3481 cccaaatatc attgcgtggc gtgttttgct gtaattcctt gagaacaaca gcgggtaaat
     3541 tatcccaact aactaatgca ctctctaaaa agtcccagtc ttccaaacgt atcagggcgg
     3601 cggcatttaa tagcatctgg tcatcaactt cgccgtggct aatcagctca gacaaaatca
     3661 tatcagcgct ggtttcatat gcatctgaat ctgctttcgg attatttttc cgatgcgata
     3721 aagcacgttg ccaaattttt tgtttcatcg acgtgctcag atactgattt ttcgccagat
     3781 tactccaccc ctcaggatcg ttgaaacgtg tcaataaccg ttgtaaagca gcgtcagagt
     3841 gaattacctg catatcccga cagcaaaagt aggcagggga ttttttctcc atccttttaa
     3901 caaaattaaa ctgtaagtta tctggcaatg ctaaaaataa cacacgtatt agatcatcgt
     3961 cattctcgaa tttttggtat aaataggctg caactttgtc actctcttgt ggcgtcatcc
     4021 gcaataattg accatggtgt ttcatctctg ccagcttgaa cgctaagctt gtgacaacat
     4081 tgtttgatga atcgttcgcc aactgcattg cctgattgta agtgagttgt cctccagcac
     4141 cgacctgacg aatagaggaa tcatcactgc ggatatagcc atcaataatg gattcgggca
     4201 ataaccacgc atctttataa agctcctgaa tgatcatatc ccaatctctt ttcgccagag
     4261 tcatgacgat atccagaggg agattttcac gtagcgcaag attcaacgga ccgacaccat
     4321 caggtagacg actcattcgc gtcattattt cgacagagag cggttcacgg tcgagcaacc
     4381 ccagaacaac aggctggtaa cccaattgct gatcggtcaa ctccagaatc tcattgtcag
     4441 gcatatatct cgccatactt tcaatcgttt gttcatcatt gctattaata agttgtttgc
     4501 gtaggttagc tgaaattcct ttaagccttg ggagagttcg cattatggca agatcaaatg
     4561 tcgcaatatc tcgtccttca aatgcagcaa cgttgccttc atccagcatt tgcaattcct
     4621 gcactaactc aggagaaggc ttagggcgaa acagcgcttc ttttttcatc tgattacaga
     4681 cattccctgc catgcgaata ttgcttcgct taaaaatttc tttcagagca tcttcatcat
     4741 ttattagacc ccaataatat gcatcaggat ttattatcat cagcgctttt ttgacatttt
     4801 cagtgtcatt tttgtcactc gcacggacca tgaattcact atttgtcgaa gacgcaacaa
     4861 caggtgcaat tatgtcattg ataaataccc cacgcgcatt aatactcccc ttaatcgtag
     4921 acggattatc gtaatatgag tcgataaata ccagtccact tgcatttatc gaaccggtaa
     4981 ttaacagtgg tgaatccgta actatattcg ttgccgtcac gttaccgaga ataacccatg
     5041 gattagaaag agagaaagtg gaaatattac ctttgactgt caaattgcca agaac
//
LOCUS       DAJTUP010000182         5062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTUP010000182 DAJTUP010000000
VERSION     DAJTUP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.185"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            111..368
                     /locus_tag="OO468_003863"
                     /pseudo
     CDS             111..368
                     /locus_tag="OO468_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(418..1368)
                     /gene="uspE"
                     /locus_tag="OO468_003864"
     CDS             complement(418..1368)
                     /gene="uspE"
                     /locus_tag="OO468_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR6260791.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1520..2272)
                     /gene="fnr"
                     /locus_tag="OO468_003865"
     CDS             complement(1520..2272)
                     /gene="fnr"
                     /locus_tag="OO468_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR6260792.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2467..2982)
                     /gene="ogt"
                     /locus_tag="OO468_003866"
     CDS             complement(2467..2982)
                     /gene="ogt"
                     /locus_tag="OO468_003866"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR6260793.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(2993..3136)
                     /locus_tag="OO468_003867"
                     /pseudo
     CDS             complement(2993..3136)
                     /locus_tag="OO468_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3526..4341)
                     /locus_tag="OO468_003868"
     CDS             complement(3526..4341)
                     /locus_tag="OO468_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6260794.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4438..>5062
                     /locus_tag="OO468_003869"
     CDS             4438..>5062
                     /locus_tag="OO468_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR6260795.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDL"
BASE COUNT         1342 a         1156 c         1211 g         1353 t
ORIGIN      
        1 ttgattgtaa atccagctaa gaggtgaggt tttcagagca gacaacggtg aaacgtcatg
       61 gtattgttac gtttaggtaa caagaaattt gtctgcacaa ggattacatc atgattatgg
      121 cgaaactgaa gtcagcgaaa gggaagaaat ttctctttgg tttgttggcg gttttcatta
      181 ttgcggcgtc ggttgtgact cgcgcgacca tcggcggcgt tatagaacag tacaatattc
      241 cgctgtctga gtggacgaca tcaatgtatg tgatttagtc atcgatgatt tttgtttata
      301 gcctggtctt tactgtgttg ctggcaatcc cgttgggaat ttatttcctt ggcggcgaag
      361 agcagtaagt aaaaaatagg cccgataact cgggccttgt cagttattga agagtcgtta
      421 atcgtcttct tcgtcatcca gttcaacggg tgtctgatac tggtcaggtt taatgaccag
      481 caggtcgcag cgaagatgat caatcacctg ttccgccgtg ttgccgagga atgctgctga
      541 aataccggtg cgtccgaccg tgcccagaac cacaattccc gcctgtaaat gctccgccaa
      601 atcaggaatc acctcttcgg gcagaccttt ttctacgtgc gtcatgtttt cattaatgcc
      661 gaatttctgc cgcagggctt tcattgccag caaatgttgc ccacgaatgg catcgttata
      721 aacgctcggg tcaaattccg gcagttcaat cgcgatatta attggcgtta ccggataagc
      781 gccaaccaga tgaacttcgg tatggttgac ttgttctgcc agttcgatcg tctctttgac
      841 cagtttttca ttgagcgcat tatgatacgg ctcttcactg gcgagattca ccgccaccag
      901 cgccttgcct ccttccggcc acggctggtc tttcaccatc cacaccgggc ttgggcattt
      961 gcgtaacaga tgccagtccg ttggcgtaaa aatcaccgct tccagacggt catgttggtg
     1021 cgccattttt agcaccaaat cgtgtccgcc gctgatcact tcctgaatga tggcttcgaa
     1081 aggacggtta tgccagacca ctttaatttc aatgggaacg ccagcattga gataatattt
     1141 tgcctgctcg tggatccagg ctgtacgctg gctgatgacg ccctgacgca tagcggtacg
     1201 ttcgtccggg gagagcaggg tggtcatttc gtatgagaag tcatagatcg gcaaaaaggc
     1261 tttaattttg ccaccaatcc gttgatgtaa ataaacagct cgccgcaatg ctggttggtc
     1321 gtcctggtta ggatcgataa caacgagcat gttctgatac atagccatac agggtctcct
     1381 tacaacaact gtcaacgcag tttgtaatta aaagattaac ccatatctgg tgaatgaaac
     1441 agtgatgaac cttctgccag atcaataaat cagaaaaatt taatgatatg acagaaggat
     1501 agtgagttat gcggaaaaat caggcaacgt tacgcgtatg gccagcaagc tgggccagcg
     1561 catcgttatt ttcgatggtg atgtatttac ctttgactgc cagcataccg cttttctgga
     1621 agcgacccag cagacggctg atggtttcta ccgtcaggcc cagatagtta ccaatatcgc
     1681 cacgagtcat cgtcaggcgg aattcacgag gggagaagcc gcgttgggca aaacgacggg
     1741 acaggttgta gatgaatgca gccagacgtt cctcggcatt tttcttcgac aacagcaaga
     1801 tcatgtcctg atcgcctttg atttcaccgc tcatcagacg catcatctgc tgacgcagat
     1861 tcggcatttt accggacaaa tcgtccagcg tttcgaacgg aatttcacat accatcgagg
     1921 tttccagcgc ctgagcgaag ctcgggtgat ggccgctgcc gatggcgtca aatcccacta
     1981 ggtcgcctgc taaatggaaa ccagtgattt gctcgtcgcc ttgctcagtg attgtataac
     2041 ttttaatcgt accggagcgg atggcataaa gcgatttaag ttcatcacca gccttaaaca
     2101 gcgtctggcc tttctgaata ggcttcttcc gctcaatgat attatcaagc tgatcaagct
     2161 catgttcgtt gagtgtgaac gggatgcaaa gctggctgat gctgcaatcc tggcaatgga
     2221 tagcacaacc gccagactga atgcgccgta taattcgctt ttccgggatc ataggtctgc
     2281 tcaagccgta attgatattt gtcaatttta acatcttttt agggagcaag taagtctaag
     2341 caaaccttaa cagcagagaa ttccgatatt agatgtaaat atatgtctat ctatttgaaa
     2401 ctccttaagt tgtcaagggt aactttacat aaaagtgtga acaagctggc acaaattgtt
     2461 taatgtttac agcaaaagat aaccttcgtg gcgcaataac cactcttttc gctgaactcc
     2521 gcctgcatat ccggtcatgg tgccgtttcg gccaataacc cgatggcaag gtacgacgat
     2581 gctgatggga ttcgatccgt ttgccgcacc aacggcacgc gccgcgccag ggcggcccaa
     2641 ttgttcagcc agttggccgt aatgcattac ctgcccgcag gggatagtgc gtagtgtttt
     2701 ccagacttcg cgctgaaatg gcgtcccccc cgtggcagtg ggaagcgtat caataatgct
     2761 aagattaccg gcaaaatatt cacgaagctt gtcgcttaaa ccacctgggt tggtggcaga
     2821 aatgcgctca tagccttctt tgcgataatg gatgtccagc agctgcacca tgcgttcgct
     2881 gtactcttcc cattcaaccg cccgcaggcg aaattgctca tcgcaaatca cccacagtgg
     2941 acccagtggc gtggcaattt tttcttcaag taatctcagc atccgttctc tcttaagaca
     3001 aacgtgggta aataccctga cctatcggca gaccgacaag ataccacgcc agcaacatca
     3061 gcagccatac caccaaaaag ataagtggat agggcaagac taacgaatag taagtaccca
     3121 gtttcgcgtc tggtttgtcg cagtctgata gcataaagcg attttttaat ttttctgcct
     3181 gaagaaatga gggcaagcca tcacatggat cgagggtggg ataattccag tcagtgaaat
     3241 tttaccgtag gctatctgtt atttgattct gtggcgtgat agccgccaga caccactgaa
     3301 ctcgtcactc attccccaat cccggcgaga atgtggttat ttacagagct aaaaaatgaa
     3361 taaaaaaata gagcctgtcg acatccgcca gactctacag tacacacagc agtgcatcct
     3421 cgtcttaaat ccggcctggg gtccaggtga ccggtgacag tgttataacg gttttaccca
     3481 tccgttgcca attttcttca gtgacagatt tcacgaaaat ataagttaaa tcatttgtct
     3541 tgtgattgca taattatgca tcgttaatcg acgagtaaat ttaccgggcg agtagcccgt
     3601 tcgacgctta aacatattgc cgaacgattc agctgaggca tagcccagac gatgggcaat
     3661 tttggtcaac gaatatccat catcaagaag cgtcactgca gaaataacgt gcatttgctg
     3721 aacccagtgg cgaaatgtta atcccgtctc tttaacaaat aagcgcatga gtgtacggct
     3781 actgacgtta attttatccg ctagattatc cagtgttggc agataccctt ggttgtttag
     3841 caaattctca caaagctgct gtagccgctc atcatttggc caagggagtt caagtgacat
     3901 tttcaccccc atttttattt cctgaagtat cagtgtctcg agggctgaaa aacgtaactg
     3961 ttgtttatct gtggtacttt gcgacggtgg aagttggtta attgcaatta gtaattccct
     4021 taacaggggt gaaatacccg tcatgaagca aactgttccc agcgtttcac tttccgcttc
     4081 tgaaaccagt acggttttta aaagtacgtt gcctgtcgca acaaacttat gcggcatttg
     4141 tgggggcagc caaagcgcgc tgctggcatg gactagccag acgctttctc cagcccatac
     4201 acgaacgact ccttcagccg ggtaaagcaa ctgggcatag agatgtttat gccagggaac
     4261 tgtacctcca ttcagatgtt ccgaatccag tccgataatg tgatgccctg gtgatgcttt
     4321 gggttgcagt gtaattgtca tttacagtag gtattttgtc agaaatcatc aaataagcca
     4381 tgttaaattt taaatatgac gtatgcaagt ttccggatat atacggtgta aagagtgatg
     4441 ataatttcta aaaaacagct tattggggtg gtagcgatcg gtattctttt gactggcgtt
     4501 gtctttttta tctggagggt aagcaaagga agatttatac agacgacgga tgatgcttac
     4561 attggtggta atatcaccac ggtggcaagt aaagtttctg gttatatttc tgctatagag
     4621 gtgcgcgata atcagagcgt aaaaaaaggc gatattatcc tgcgtctcga tgatcgggat
     4681 tatcgtgcaa atgtcgccag gcttgaggca aaaataaaaa gtagcaaagc caatctcgaa
     4741 ggcattcaag ccactattac gatgcagcag tcgataattc aaagtgccag tgaaacatgg
     4801 caggcagtaa aacatgagga gcaaaagcgc ctgcgagata ctgaacgtta tgaaaaactc
     4861 gcacaatcag cggcaatatc gcaacaaatt attgataacg ccaggtttga ctaccagcag
     4921 gtcgcagcaa aggagcgcaa agccgcgaac gatttcctgg tggagaagca acggctggca
     4981 gttttatctg ctcaggaaga aaacgtacgt gcgagtatcg aagaagttca ggccgctttg
     5041 actcaggcac tactggatct gg
//
LOCUS       DAJTUP010000183         5015 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTUP010000183 DAJTUP010000000
VERSION     DAJTUP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5015
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.186"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO468_003870"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO468_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR6260796.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO468_003871"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO468_003871"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR6260797.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO468_003872"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO468_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR6260798.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO468_003873"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO468_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR6260799.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5015
                     /gene="yidZ"
                     /locus_tag="OO468_003874"
     CDS             4378..>5015
                     /gene="yidZ"
                     /locus_tag="OO468_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR6260800.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQ"
BASE COUNT         1142 a         1204 c         1254 g         1415 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagag
//
LOCUS       DAJTUP010000184         4925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTUP010000184 DAJTUP010000000
VERSION     DAJTUP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.187"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(12..953)
                     /locus_tag="OO468_003875"
     CDS             complement(12..953)
                     /locus_tag="OO468_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR6260801.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(965..1454)
                     /locus_tag="OO468_003876"
                     /pseudo
     CDS             complement(965..1454)
                     /locus_tag="OO468_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1458..2678)
                     /locus_tag="OO468_003877"
     CDS             complement(1458..2678)
                     /locus_tag="OO468_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR6260802.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2682..3425)
                     /locus_tag="OO468_003878"
     CDS             complement(2682..3425)
                     /locus_tag="OO468_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR6260803.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3316..4782)
                     /locus_tag="OO468_003879"
     CDS             complement(3316..4782)
                     /locus_tag="OO468_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR6260804.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
BASE COUNT         1040 a         1396 c         1247 g         1242 t
ORIGIN      
        1 ccatgtggct gttaaatgcc atcaacgtac gaaatggttt ctttgtacac tggctcgact
       61 gcacccagct tgccgtagta agtgacgatc tgatacagac cgcgatactg caccggcacg
      121 ctctgaagcg gaaccagcgg gtagcggacg tattttttat cgttggtgta cgcaactatg
      181 cgatccttat tccccacacc acggcctttc agccatttaa ccgcgcggat attcagcgga
      241 acaccgttct ggtgatagct gatggtgttg gtctgaaggt tcgtcaacag ggactggtta
      301 cctgcagatg aaacgatgat gctggacaac agagcaaact gctcaggcgg gatcagcaaa
      361 tcacgcggaa ccacagagta accagaagcg gcccacgcat cagacagcac ctggttaatg
      421 cttgcgcgga tttcgtccgg tgttgaggtt gcccacgttt tggcagcgtt gttgacaggc
      481 acgccgtcca gggtaacaag gcctttcagg tttaatgcgg aatcgccaac atatacctgt
      541 tcatcgttat ccatctgcca tttcagttgc ataccgtcat acttctgcgt atcaatcggg
      601 cggccgacct gctgagcagc ctgcaattct atgaccgtcc agccaagttc catcccccac
      661 aggttcagcg ggttaccgga tttgccgata tccacgttca cgccagcaat agcggttgag
      721 tctttgccta cccagttttt tccattcgga tttgcgccag tacccgcagc ggcgaagctg
      781 gtattcgtcc agctggaaat gtcatctgcg atggaggcgt cttcacgcag ttgaatatcg
      841 cggctccagg tgtaccccac cagtggcagg ttcagcgtct ggtcgagtct ctccagctcc
      901 ccgatgagaa aggcaccaga gctgtcaacg gttgcctgat caaaagtaat cattcgtctg
      961 ttccttaaat cttccaggaa atttctgcat tgccgtcagc atcaccggca cctgtgaatt
     1021 cagcgttggt cagcaccacg tttttgccac tgactgacgt ggacatgaat ccacccagag
     1081 gcactttgat ggattcatca gtggagatcg ccttttttga tagtgctggc atcaaaatca
     1141 gaaccgagat taacggtcac gtagccacgc ttcatggcgt cgcccgggaa gttcttgcct
     1201 gttcccacct ggcgaaccat gtccggctgc gacgtggtcg gataagggcg cacgtagatc
     1261 cccttcacct tgtctgcggt atcaccatct gccagcggca cgaaaaaacc gtcatcatcg
     1321 tatttaccag ccaggccata ggcagcgaag gcgttatcgg atttaaggac caccggttcg
     1381 acggttaagt cctgcgggcg agagacagcc ccggcaatgc caacaggcat ccggtacaga
     1441 aatacattat tcatttttta ccctttacgg tttgcccaga attcagcgtt ttgtttgttc
     1501 agggaagcga tactggtcat gcccatgttt aggcgctgtg catcgccggt ggtggcgcgg
     1561 gtgtttcgcc ctttggcaac ctcagacacg gcattaaacg ccatgtcgac cgattgtttc
     1621 ggtaatttgc ggatatccgc atcaccgact atctggcgaa ccagcgtttt gtcagcagaa
     1681 gccagaacct cgcgtttgaa cgcggtcggt ttcatcttac ggctcagatc gatacccgga
     1741 acgataactt cggcacgcca ggctgagtca ccagtaatcg tggtttcctc ttcatcgtcc
     1801 tcgccgtcac cggtcggatt atcgtcaggc ttattatcgt tatcgcccgt ggcatttctt
     1861 tccagcttag ccagcagggc tttcagtaat gttttgaggt catcatcact gtcgccggtt
     1921 ggatctccgc ccatctctgg tgctttgtcc ggtagtggtt gctgcgggga caggttgatg
     1981 ttgagattaa cgccctgcgg caaatccccc tcatctcctg taaccgatgc gggagccgac
     2041 tccaccagtt cgttcatggt gtcggcatct cctgtcttga tggccgcacg catgcggttc
     2101 caccagtttt tcttttgatt tgccattgtg tctctgtctc caattgcaca acgatttccg
     2161 gctctgcctt tagggacaag agccacatgg tttccggtaa tatcgacctg ctcggcttta
     2221 cctggctcgg tctgctcgta ctccgcgtca tagccgcacg acacttcgcg caggccatct
     2281 tcgataagct gaatggcgct ttcgtctttg acgataaggt cagccagcat caaatcagac
     2341 tgctcacccg tcccgcgccg gacattctgg aggtgcccga cagcaagcac tttccagttc
     2401 tcgggattta ccagccgcac attcccgttt tcatcttcag gatgcaggat cgtgatgctc
     2461 atcccttcga atgaggcaag cgtggccgga tggaatacct gctcaggaga acgcgtgacg
     2521 actatttcaa cgaacttatc gggtttcagt tttggcaggt catcagcacc atagagctgc
     2581 ttacctgttc gtcctatcgg cacgtctttg cacagcaacg agccgtcagc caggcggtat
     2641 cgggtttccc cgagcctggt ctcaaaaaag tatttcatgg gttatgctgc ccttttcatt
     2701 gcgaatggaa cgcgaacaaa cacgatctct ttatagcagc ggcagtttgg cagctcgccc
     2761 gcgtggccgg tcatgccgtc caaagttgga ggtgaatccc agcggacgaa tttacctttc
     2821 atgtgatcgt gggaatctcg gacatcgcca tcatcggctg tacgccaaat ataaccatcc
     2881 gagccaatag ctatcgctcg ggccatatcc agcgcgcccg ttgctcttcc cagttccgtt
     2941 cgggcaatca gatcggccct agacttagca acatcgccgg tagatgcaat ctctttagca
     3001 aactgctccg cccgaccacc ggtgacaaca gcctctatcg ctttgttctg gatgtcgtac
     3061 accctgtctg cggcctcgag aggcagagat ttgatgtact tgacctgttc ggcgatgatg
     3121 gattgcatca cctggcccac aggagcgctt tccacaagat tgcggagctc gcgactgatg
     3181 ttcttgctgt gttgccgcca aactttctcg ttctgccggg ttaggtccgc agtaaagttt
     3241 tccgcgacct ttgtcgccca gggggtgatg atttcactgt aacgctccag cgccgcaata
     3301 atttccgtga tactgtcatt tgaaccatcg tagcgaccat ttacgatgtc tccgaccgcc
     3361 ctcgctatcc tgcgtaggct ggttcgatac cggatttccg cctgacggct cctgcggttc
     3421 gtcatcaggt tcgccgatgc cgggcggcgc ttcatcttcg gcattctcga tgtcctcgtt
     3481 ggtaatggat gcccctatgc cggttacgtc agaattttct cgcaaatcag tcatagcggc
     3541 tttcagtgtc atcagaccat cacccagcgc cgtactgatt gcgttggtgg tgtttaacgc
     3601 caccgttgag cgatcgacat cagacatttg ccagagcggg ttaaactcaa acgtgaaatc
     3661 gtccggcagc ggcttgccaa gttccgaacg atgcatgatg tccagtatcc gccgcaccgg
     3721 aagacgtaaa cgcctctcct gcaacgaact gatgcgatcg taatagttgg caaggtctgc
     3781 atcaccggta gaaaatcctt tcggggactg tccgaacaac cgcaccagtg ggataccaac
     3841 agcgccacta atctgttctg caaactgcga aaggatgtca tccagaccac tgaagctgta
     3901 ctgatgggtt tcaaacttat cccgcgagtc catgagcgtc atgccttcat tactctggaa
     3961 ctgtcgaatc aggtcgatat tcttcagcaa cgcttcatac gcaggaccac caagtgcgat
     4021 aagctcgcgt agcttctcca cgctgtaggt acgcaaatgc gccttgtaga ccagctgcgc
     4081 cgcgccgaca gtagcgctgt cgaacgcggt aagacgatcc cagatacgct ctacaaccga
     4141 cattccccat tcgttctcgg tcatcttctg ctgaaatggc aacgtgacgc catcaaagcg
     4201 aatcaggcga ctgtgatgaa tgcgccaggc aggaattccc gttgctgtgg tcaccacatc
     4261 gtaaaactca ggtttaccca ggtccggccc catatcttta atgcggcggg tcagtgccgg
     4321 gtcgaccatc cagcggtcga gcgggagaat ccccttaaac ttgcccttac cgatggtttc
     4381 gggtcgcagc ggggtcattg gtgcctgccc ctcaatcatg atgaaaccca ccgcgccgcc
     4441 gtagaggcgc gaccatttca gcacgtcatt cagcgcatcc cagatttgca actcatccag
     4501 ttgtgattcg agaatgccac gatcttttgc atcaatttcc gaagtgatgc gaatgccttt
     4561 gcgggtcata tcatccggga tagcatcgac tgcttcgccg atgatccagg atgaacgata
     4621 ggaccattcc accagcatgc ggttacgact ggtgaaatta gcccggtagg tggatgctga
     4681 gtgctggtta ggtgtctgca tccctactcg ggcaataaaa ttctcataac catcagctgt
     4741 ggcctgcaca gttcgccgca gggcttgttt gtttcgtgcc atcaggcctg tctccccagc
     4801 tgttcccaga tatccagcga tgtatcgatt ggcgcgaagg ccataatgaa tgcatcagca
     4861 acgtttggtg acggtatctc gcgttttgcg aggtcttttt tactttcgac catcacacgt
     4921 ccgtt
//
LOCUS       DAJTUP010000185         4907 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTUP010000185 DAJTUP010000000
VERSION     DAJTUP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.188"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..2126
                     /gene="mutS"
                     /locus_tag="OO468_003880"
     CDS             <1..2126
                     /gene="mutS"
                     /locus_tag="OO468_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR6260805.1"
                     /translation="TLDISSGRFRLSEPADRETMAAELQRSNPAELLYAEDFAEMSLI
                     EGRRGLRRRPLWEFEIDTARQQLNLQFGTRDLVGFGVENAPRGLCAAGCLLQYAKDTQ
                     RTTLPHIRSITMEREQDSIIMDAATRRNLEITQNLAGGAENTLASVLDCTVTPMGSRM
                     LKRWLHMPVRDTRVLLERQQTIGALQDFTAELQPVLRQVGDLERILARLALRTARPRD
                     LARMRHAFQQLPELRAQLETVDSAPVQALREKMGEFAELRDLLERAIIDTPPVLVRDG
                     GVIASGYNEELDEWRALADGATDYLERLEVRERERTGLDTLKVGFNAVHGYYIQISRG
                     QSHLAPINYMRRQTLKNAERYIIPELKEYEDKVLTSKGKALALEKQLYEELFDLLLPH
                     LEALQQSASALAELDVLVNLAERAYTLNYTCPTFIDKPGIRITEGRHPVVEQVLNEPF
                     IANPLNLSPQRRMLIITGPNMGGKSTYMRQTALIALMAYIGSYVPAQKVEIGPIDRIF
                     TRVGAADDLASGRSTFMVEMTETANILHNATEYSLVLMDEIGRGTSTYDGLSLAWACA
                     ENLANKIKALTLFATHYFELTQLPEKMEGVANVHLDALEHGDTIAFMHSVQDGAASKS
                     YGLAVAALAGVPKEVIKRARQKLRELESISPNAAATQVDGTQMSLLSVPEETSPAVEA
                     LENLDPDSLTPRQALEWIYRLKSLV"
     gene            2232..2884
                     /gene="pphB"
                     /locus_tag="OO468_003881"
                     /pseudo
     CDS             2232..2884
                     /gene="pphB"
                     /locus_tag="OO468_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(2935..3702)
                     /locus_tag="OO468_003882"
     CDS             complement(2935..3702)
                     /locus_tag="OO468_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR6260806.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            3898..4806
                     /locus_tag="OO468_003883"
                     /pseudo
     CDS             3898..4806
                     /locus_tag="OO468_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            4803..>4907
                     /locus_tag="OO468_003884"
     CDS             4803..>4907
                     /locus_tag="OO468_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260807.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVP"
BASE COUNT         1193 a         1259 c         1283 g         1172 t
ORIGIN      
        1 cgacgctgga tatcagctcc gggcgttttc gcctgagcga accggccgac cgcgaaacga
       61 tggcggcaga gctgcaacgc tctaatccgg cggaactgct gtatgcagaa gatttcgccg
      121 agatgtcgct gattgaaggc cgtcgcggcc tgcgccgtcg cccgctgtgg gagtttgaaa
      181 tcgacaccgc gcgccagcag ttgaatctgc aatttggcac ccgcgatctg gtcggttttg
      241 gcgtcgagaa cgcgccgcgc ggactttgtg ctgccggttg tctgttgcag tatgcgaaag
      301 atacccaacg cacgaccctg ccgcatattc gttctatcac catggaacgt gagcaggaca
      361 gcatcattat ggatgccgcg acacgtcgta acctggaaat tactcagaac ctggcgggcg
      421 gtgcggaaaa tacgctggct tctgtgctcg actgcaccgt cacgccgatg ggtagtcgta
      481 tgctgaaacg ctggctgcat atgccagtgc gcgatacccg cgtgttgctt gagcgccagc
      541 aaactattgg cgcattgcag gatttcaccg ccgagttgca gccggtactg cgtcaggtcg
      601 gcgacctgga acgtattctg gcacgtctgg ctttacgaac tgctcgccca cgcgatctgg
      661 cccgtatgcg ccacgctttc cagcaactgc cggagctgcg tgcgcagtta gaaactgtcg
      721 atagtgcacc ggtacaggcg ctacgtgaga agatgggcga gtttgccgag ctgcgcgatc
      781 tgctggagcg agcaatcatc gacacaccgc cggtgctggt acgcgacggt ggtgttatcg
      841 catcgggcta taacgaagag ctggatgagt ggcgcgcgct ggctgacggc gcgaccgatt
      901 atctggagcg tctggaagtc cgcgagcgtg aacgtaccgg cctggacacg ctaaaagttg
      961 gctttaatgc ggtgcacggc tactacattc aaatcagccg tgggcaaagc catctggcac
     1021 ccatcaacta catgcgtcgc cagacgctga aaaacgccga gcgctacatc attccagagc
     1081 taaaagagta cgaagataaa gttctcacct caaaaggcaa agcactggca ctggaaaaac
     1141 agctttatga agagctgttc gacctgctgt tgccgcatct ggaagcgttg caacagagcg
     1201 cgagcgcgct ggcggaactc gacgtgctgg ttaacctggc ggaacgggcc tataccctga
     1261 actacacctg cccgaccttc attgataaac cgggcattcg cattaccgaa ggtcgccatc
     1321 cggtagttga acaagtactg aatgagccat ttatcgctaa cccgctgaat ctgtcgccgc
     1381 agcgccgtat gttgattatt accggcccga acatgggcgg taaaagtacc tatatgcgcc
     1441 agaccgcact gattgcgctg atggcctaca tcggcagcta tgtaccggca caaaaagtcg
     1501 agattggacc tatcgatcgc atctttaccc gcgtaggcgc ggcagatgac ctggcttccg
     1561 ggcgctcaac ctttatggtg gagatgactg aaaccgccaa tattttacat aacgccaccg
     1621 aatacagtct ggtgttaatg gatgagatcg ggcgtggaac gtccacctac gatggtctgt
     1681 cgctggcgtg ggcgtgcgca gaaaatctgg cgaataagat taaggcattg acgctgtttg
     1741 ccacccacta tttcgagctg acccagctac cggagaaaat ggaaggcgtc gccaacgtac
     1801 atctcgatgc gctggagcac ggcgacacta tcgcctttat gcacagcgtg caggacggcg
     1861 cggcgagcaa aagctacggt ctggcggttg ctgctctggc gggcgtaccg aaagaggtta
     1921 ttaagcgcgc acggcaaaaa ctgcgtgagc tggaaagcat ttcgccgaac gccgccgcga
     1981 cgcaagtgga tggtacgcag atgtctttgc tgtcagtgcc agaagaaact tcgcctgcgg
     2041 tcgaagcact ggaaaatctc gacccggatt cactgactcc gcgtcaggcg ctggagtgga
     2101 tttatcgctt gaagagtctg gtgtaataac aattcccgat agtcttttgc tatcgggaat
     2161 attaacgaca actgacgaat caaataaaaa taccctgtat aataggaaag cttattttac
     2221 agggtaaaac catgccatct acacgctacc aaaaaatcaa tgcccatcac tatcgccata
     2281 tatgggtcgt tggtgatatt catggtgaat atcagttatt acaatcccgc ttacatcaac
     2341 tctctttttt ccccgaaacc gacttactta tttctgtcgg cgataatatt gatcgtgggc
     2401 cggagagtct tgacgtcctg cgcctgctaa accagccctg gtttatctcc gttaaaggca
     2461 accacgaagc aatggcgctg gatgcgttcg cgaccggcga tggcaatatg tggcttgcca
     2521 gcggtggtga ctggtttttc gatttaaatg attgcaacaa gaggcaatag ttctgttgct
     2581 gaaattccat caccttccac atattattga aatcactaac gacataataa aatatgtcat
     2641 cgcacatgca gattatccgg gtgatgaata tctctttggc aaagaaatag cggagagcga
     2701 attactctgg cctgtttatc gtgtgcagaa atcgcttaat ggcgagttac aacaaataaa
     2761 cggcgctgat tattttatat ttggacatat gatgtttgat aacattcaga cgttcgctaa
     2821 ccagatttat attgataccg gatcgccgaa aagtgggcgg ctgtcatttt ataaaataag
     2881 gtagtctcat gcttcttctg tgaagcatga gtaaccagat gttatttcag gccattattc
     2941 atttttcgct accagcaaag agagatcctg cttcgccagc gcgcgactgg cactctccgg
     3001 caaaccgtcg tctgtaataa tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc
     3061 cacctgacca tatttcgtcg catcacagac caaaactcgc tggcggctgg cactggcaat
     3121 cgcccgtttc accgtgactt tatcttccgc tggcgtagaa atcccccgca cactccatga
     3181 cgatgcagaa ataaaagcct gatcaatcat caggctgcac agcatggtcg cagcggcttc
     3241 cccgacacag gaacggtttt cccgacacac tgcaccgcca gtgtgaataa ttgtgcaatt
     3301 actgttgtcg agcaagtagt tcgcaataac gaaatcgttt gtgaccacag tcagtgactc
     3361 catgtgaatc agatgctgtg ctatcgctaa cgtggtcgtt cccgcatcca gatagataca
     3421 acttcccggc agaacaagac ttgccgccag cttgccaata gccgcttttt gcgtcattgc
     3481 cagcgcagtt tttacctgat gagaaggttc atgcgccacg cgtcccggag actggacgcc
     3541 tccggacacc agcacaacgg ctccctgctg ctccagtttt tgtaaatccc gacgaatggt
     3601 catatgtgac acattaattc tgtccgttag ttcagcaata ctgacaatgc ctttttcagc
     3661 taccatctca aggatgattt ggcgacgctc tacgggtatc aacttttgct ccttcctttg
     3721 tcctgctgac attctacgct atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt
     3781 tcacataaaa taacacaaaa tgttaattta tgtgaatcat ctcaccatat cgttatctcc
     3841 cagcgcttat gttcacaata tcaaacaaaa tatcacttaa attaacaagg agagcagatg
     3901 aaaacgggat ctgagtttca tgtcggtatc gttggcttag ggtcaatggg aatgggagca
     3961 gcactgtcat gtgtccgcgc aggtttttct acctggggcg cagacctgaa cagcaatgcc
     4021 tgcgctacgt tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc gacgtttgcc
     4081 gaaaaactgg acgctctgct ggtgctggtg gtcaatgcgg cctaggttaa acaggtgctg
     4141 tttggtgaaa caggcgttgc acaacatctg aaacccggta cggcagtaat ggtttcttcc
     4201 actatcgcta gtgctgatgc gcaagaaatt gctaccgctc tggctggatt cgatctggaa
     4261 atgctggatg cgccagtttc tggtggtgca gtaaaagccg ctaacggtga aatgactgtc
     4321 atggcctccg gtagcgatat tgcctttgaa cgactggtac ccgtgctgga agccgttgcc
     4381 ggaaaagttt atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa aattattcac
     4441 cagttgttag cgggcgtaca tattgctgcc ggagccgaag cgatggcact tgcagcccgt
     4501 gcggggatcc cgctggatgt gatgtatgac gtcgtgacca atgccgccgg aaattcctgg
     4561 atgttcgaaa accggatgcg tcatgtggtg gatggcgatt acaccccgca ttcagccgtc
     4621 gatatttttg ttaaggatct tggtctggtt gccgatacag ccaaagccct gcacttcccg
     4681 ctgccattgg cctcaacagc attgaatatg ttcaccagcg ccagtaacgc gggttacggg
     4741 aaagaagacg atagcgcagt tatcaagatt ttctctggca tcactctacc gggagcgaaa
     4801 tcatgatcaa gattggcgtt atcgccgatg attttaccgg cgcgacggat atcgccagtt
     4861 ttctggtgga aaacggtcta ccaacggtac aaattaacgg tgttcca
//
LOCUS       DAJTUP010000186         4647 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTUP010000186 DAJTUP010000000
VERSION     DAJTUP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.189"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(700..1116)
                     /locus_tag="OO468_003885"
     CDS             complement(700..1116)
                     /locus_tag="OO468_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260808.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1143..1283
                     /locus_tag="OO468_003886"
                     /pseudo
     CDS             1143..1283
                     /locus_tag="OO468_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1283..2308
                     /locus_tag="OO468_003887"
     CDS             1283..2308
                     /locus_tag="OO468_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6260809.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2361..2450)
                     /locus_tag="OO468_003888"
     tRNA            complement(2361..2450)
                     /locus_tag="OO468_003888"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2414..2416),aa:Ser,seq:cga)
     gene            2544..3341
                     /gene="mtfA"
                     /locus_tag="OO468_003889"
     CDS             2544..3341
                     /gene="mtfA"
                     /locus_tag="OO468_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR6260810.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3442..3517
                     /locus_tag="OO468_003890"
     tRNA            3442..3517
                     /locus_tag="OO468_003890"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3475..3477,aa:Asn,seq:gtt)
     gene            3679..4647
                     /locus_tag="OO468_003891"
                     /pseudo
     CDS             3679..4647
                     /locus_tag="OO468_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         1280 a         1098 c         1028 g         1241 t
ORIGIN      
        1 taaacagatg acgataaggg cgattaacat cgccttttgc tgcttcatag cctgcttctc
       61 cttgcctttc ggcacgtaag aggctaacct acatgtgttc agcatggatt gagcctcaga
      121 ttaatgttaa gcgtcttgca ggacgcgtaa tgttaactgg ggcttttctc tatctgcctt
      181 ttggtgttca tgcctgagac agatagcctc aagcacccgc agtcattcta cttaactaag
      241 atttccctgc aaaccgtttt tgtccggcac agtaaatatc caactaaacc aatagcgttc
      301 gctgtattta ccgccagtat tcaatgcaca tgaccgccat gaacacccct aaaaaaaggg
      361 catttatatg tccaaacatt aatatcaaaa catcaatttt ttccatatac cttgctgtga
      421 agatgatggg catacatgat gcgaacaacc agaacgcaac aaacaaaaac tgcaatgcgt
      481 ttttcattat tccccctaca atcaatgtgc aataacattt aaacacacct caatttggcc
      541 ggacatataa atatctaaac cagaaaaaat cacttacata gcgttacaaa ctctttagtc
      601 taaatactca tcgtaaaaca ttccccatac ttatcagtcc gttccgcgcc aggtagctta
      661 ttgccttatc tggcaaccta taatcaggtt tccgcttttt cagttggctg gtcgtttaac
      721 cgacatagtt aacccattaa tctggttgcc ggatgctggt ggattttcgc gttttaattg
      781 ttcataaaag tgcacagctt taaccagttc ttctgatgta accgggactg gtggggcagt
      841 gaataaggcc tgaatttcat agttcggcct gtcgttacaa tcctcttttt tcggtacata
      901 tttccagtca ccaacccaca actccccctg agagtccata acaccttttt tcacgtagcg
      961 atatcgccac gctatcggct ctgcttccag cgatgcccgt gcaattttga ataactcgcc
     1021 ctctactcgc gccatccctg aattggggtg gcatttcgta atcgctattt ttaatttggc
     1081 ttcttcgatt aattgttctt ttgttaattc agtcattttc attaccgccc tggtaatgct
     1141 gccaccagcg aaaatggtta atggaaaacg ggcttccgtt ctataccaat cgtagtggca
     1201 aaccgattgt cagccgggat ctatttacct gcaataaaac tttaccacca cgcgaggtag
     1261 agccgaattt tggtgcgatc tgatgggaag acgaaggaaa aatcctgaac acgaaaaatt
     1321 acctccaaat gtatacccaa ataaatatag ttatgtatgg aaaccaacat ccagagaatc
     1381 tgtaacacta accgccatca aggatggttt agctgcttta tggaaaaagt atgaggaaac
     1441 tgtaaataat cgcgatcgtg caatgacatt cggtcgcttg tgggaaaaat tcctcgccag
     1501 cgcctattac agtgacctca gtccaagaac acaaaaagat tatctgcaac atcaaaaaaa
     1561 gttgcttgcc gtattcggta aggtgccggc agattccata aaaccagaac acatccgtcg
     1621 atacatggac aagagagggg aacagagtaa aacgcaagcc aaccatgaaa aaagcagtat
     1681 gtcccgcgtt tacagttggg ggtatgagcg agggtacgtg aaggctaacc catgtgcagg
     1741 tgtaagtaaa ttcaaggcca aaaaccgcga acgatatgta accgacaaag aataccaggc
     1801 agtattaagc gttgcacctc ttcctgtttt tatcgcaatg gaaattgcct atctgtgtgc
     1861 agcgagggtt tccgatgtgt tatcgctgaa atgggagcag attggaaacg acgggatctt
     1921 tatccagcaa gggaaaacag gaaaaaaaca gataaaagca tggagtccac gattacaggc
     1981 ggcgatcgaa aaagcaaaac agttaccaac atccgcctat gtaatcagca atcaatacgg
     2041 caaccgatat atgtacaaag gctttaacga aatgtgggta gaagcaagaa atcgcgcagg
     2101 caaaatttca ggtattttaa ccgacttcac ctttcatgat ctgaaggcga aaggaatttc
     2161 agactatgaa ggaagcagtc gggataagca acttttctct ggtcacaaaa ccgaggggca
     2221 agtgctaatc tatgacagga aggttaaagt ttcaccgaca cttgatgtcc cgttacctga
     2281 aaatattcca agaaaatatt ccaagtaatt ccaagtgtga tttttgtcac tgacttaatg
     2341 atgtataagt gattgaattt tggcggagag agggggattt gaacccccgg tggagttgcc
     2401 cccactccgg ttttcgagac cggtccgttc agccgctccg gcatctctcc gttcagatgg
     2461 ttgccatgat gccaggaaat ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa
     2521 gtgacgagtt tgcgagcaaa acgatgatta agtggccctg gaaagtacaa gaatcagcac
     2581 atcaaactgc ccttccctgg caggaagcac tatcgatccc ccttttaacg tgtctgacag
     2641 aacaggaaca aagcaaattg gtcgctcttg ccgaacgttt tttacagcaa aaacggcttg
     2701 ttcctttaca gggctttgag ctgaattcat taagaagctg ccggatagca cttctatttt
     2761 gcctgcccgt tctggagtta ggactggaat ggctggatgg ttttcatgaa gtcttaattt
     2821 atcctgcgcc atttgtggtc gatgatgaat gggaagacga tatcggtctg gtgcataacc
     2881 aacgtattgt tcagtcaggt cagagctggc agcaagggcc tatcgttttg aactggttgg
     2941 atatacaaga ttcttttgat gcttctggtt ttaacctgat tattcatgaa gtcgctcata
     3001 agctggacac ccgtaacggc gatcgcgcca gcggagttcc ctttatttcg ttgcgtgagg
     3061 ttgctggctg ggaacacgat cttcatgctg caatgaacaa cattcaggaa gaaatcgaat
     3121 tagttggtga gaatgcggcg agcattgatg cttatgctgc cagtgatcct gctgaatgtt
     3181 ttgccgtact ttctgaatat ttctttagcg ccccagaact ttttgctcct cgtttccctt
     3241 cattgtggca acgtttctgt caattttatc aacaagatcc tttgcagaga ctgcatcacg
     3301 ctaatgatac agactcgttt tcagcgacga atgttcatta attaacaact ttgaagatta
     3361 attaaccaat tgaaatggct tatgaaattt agtgttgaca gacaaggtac cgctaagtaa
     3421 tatgcgcccc gttctcacga ttcctctgta gttcagtcgg tagaacggcg gactgttaat
     3481 ccgtatgtca ctggttcgag tccagtcaga ggagccaatt ttctgttttc atgcatcctt
     3541 gcgaatcttt atctgatgtt gattcaacag gttagtgtga aaactcttct cggtgcattt
     3601 tgattttacc ctacgcatcg ggaaaaattg gtggtcaaat ctgggatcag gttagttcga
     3661 taatggagtg acccccatat atcccttacc gacgcaaaaa tccgcaccct caagccttct
     3721 gataaaccct ttaaagtctc cgattctcac ggtctgtatc tgctggtcaa gccgggtggc
     3781 tcccgccact ggtatctcaa ataccgtatt agcggtaaag aatcccgcat tgcgctgggt
     3841 gcctatccag ccatctccct gtctgatgcg cgacagcaac gtgaaggtat ccgtaaaatg
     3901 ctggcgctga atatcaaccc ggtacagcag cgggctgctg aacgtggctc acgaacaccg
     3961 gagaaagttt ttaaaaacat ggcgctggcg tgacataaaa gtaacaggaa atggtcgcag
     4021 aacaccgccg accgtctgct tgccagcctg aacaatcaca tctttccggt catcgggaac
     4081 ctacctgtat cagaacttaa accccgtcat ttcattgacc tgctgaaagg gatcgaggaa
     4141 aaaggtctgc tggaggttgc gtcccgcaca cggcagcacc tgagtaacat aatgcgccat
     4201 gcggtccatc aggagttaat cgatacgaac cctgcagcaa accttggcgg cgtgaccaca
     4261 cctcctgtca gacggcacta tcctgccctg ccgctggagc ggctgcctga actgcttgaa
     4321 cgtattgggg catatcatca gggccgtgaa ctgacccggc atgccgttct gctgatgctg
     4381 catgtgttca ttcgctccag tgaactgcgt ttcgcccgct ggtcagagat tgatttcaca
     4441 aaccgagtct ggacgatacc cgcgacgcga gaacccatta ttggcgtgcg ttattccggc
     4501 cgcggggcaa aaatgcgaat gccgcatatc gtccccctct cagaacagtc catcgccatt
     4561 ctgaaacaga ttaaggatat caccggtaat aatgaactga tcttccccgg cgaccataac
     4621 ccgtataagc caatgtgtga aaacacg
//
LOCUS       DAJTUP010000187         4639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTUP010000187 DAJTUP010000000
VERSION     DAJTUP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.190"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            99..283
                     /locus_tag="OO468_003892"
                     /pseudo
     CDS             99..283
                     /locus_tag="OO468_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            426..647
                     /locus_tag="OO468_003893"
                     /pseudo
     CDS             426..647
                     /locus_tag="OO468_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(668..730)
                     /locus_tag="OO468_003894"
                     /pseudo
     CDS             complement(668..730)
                     /locus_tag="OO468_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1180..2592
                     /gene="ospC1"
                     /locus_tag="OO468_003895"
     CDS             1180..2592
                     /gene="ospC1"
                     /locus_tag="OO468_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR6260811.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2696..2845)
                     /locus_tag="OO468_003896"
                     /pseudo
     CDS             complement(2696..2845)
                     /locus_tag="OO468_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2921..4618
                     /gene="ospD3"
                     /locus_tag="OO468_003897"
     CDS             2921..4618
                     /gene="ospD3"
                     /locus_tag="OO468_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR6260812.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT         1625 a          832 c          860 g         1322 t
ORIGIN      
        1 cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg gtaattgcag
       61 accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct ttgtgtccga
      121 tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag gtgaagaaag
      181 ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt gccatcgaca
      241 gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg agcggtgtac
      301 cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac tgtccttacc
      361 cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg ttgtaaagtg
      421 aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg ttgcagacgc
      481 aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag cggatggcgg
      541 cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag cgtatgcagt
      601 tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca ttttcacggg
      661 cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg attttgaact
      721 cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc caaactaacc
      781 tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa tcatcgagga
      841 tttcattgca gaatacacat tccactaact ggaagcactc cataagagga atcagagaaa
      901 gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata gttattaaat
      961 tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga ttttagatat
     1021 tttgaaatat ctggcatcac ataacatcaa aagtatataa tactcttatc taattacatg
     1081 cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc ttgtagggaa
     1141 agatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc agaaacactg
     1201 aactcagcaa atacccaatg caatatagat tctatggata acagattaca tacattgttt
     1261 ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga aaaaaattta
     1321 aaagatagtg caaatattat taaaagtttc tttaggaaaa ctatagcagc acagagttat
     1381 agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat tgatgcacca
     1441 tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc gaagcattat
     1501 atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa tttgctttcg
     1561 ctaattatta attctgattt aatcttcaga catcaaagta attctgattt gtctgataaa
     1621 attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac aaaacgaaac
     1681 acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt tggcgttgaa
     1741 atctctaacc atcagaaaaa acaccccctg aatacaaaac atcacactgt tgattttggt
     1801 gcaaatgcgt atatcattga tcatgactct ccatatggat atatgacatt aaccgatcac
     1861 tttgataatg ctattccacc tgttttttac catgagcacc aatcattttt agataaattt
     1921 tcagaggtta ataaagaagt tagtcgatac gtacatggaa gtaaaggaat tatagatgta
     1981 ccaatattca atactaaaga tatgaagtta gggctcggat tatacctgat tgactttatt
     2041 agaaaaagtg aagaccaaag cttcaaggag ttttgctatg gaaaaaatct tgcccctgtg
     2101 gatctggata gaatcataaa ctttgttttt cagccagagt accatatacc taggatggta
     2161 agtacagaaa acttcaaaaa agttaagatt agagaaatat ccttagagga ggctgttaca
     2221 gcatctaatt acgaagaaat taacaagcag gtcactaaca aaaaaattgc tctccaggct
     2281 ctttttcttt cgattactaa tcaaaaagag gatgtcgcct tatatatatt atctaatttt
     2341 gagataacta gacaagatgt tatttccata aagcatgagt tgtatgatat tgagtatcta
     2401 cttagcgctc ataattcaag ctgtaaagta cttgagtatt ttatcaataa gggattggtt
     2461 gatgtaaaca caaagttcaa aaaaactaat agtggggatt gtatgttgga taacgcaata
     2521 aaatatgaga atgcagaaat gataaaacta ttattgaaat atggtgcaac atctgacaat
     2581 aaatatattt aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc acaaaatatc
     2641 gatatattgt ttattgtcag tatggctcaa tgtgataata gttggaaagt ttgatgggtt
     2701 tcgccccgtt gtagcggtag tcgaactgga aggtcttcag gcactgctta cagcgataga
     2761 gcagcccccc tgaactggaa tggccgttcc ggataccccc ctgagtttca gagtaacggt
     2821 gacaaaccac atcaatcttt gccatcaatc atccaaaggg caaagagtac aacaacacta
     2881 agtctgcgtc acaacccatc aatgaaagga atatatacat atgccatcag taaatttaat
     2941 cccatcaagg aaaatatgtt tgcaaaatat gataaataaa gacaacgtct ctgttgagac
     3001 aatccagtct ctattgcact caaaacaatt gccatatttt tctgacaaga ggagtttttt
     3061 attaaatcta aattgccaag ttaccgatca ctctggaaga cttattgtct gtcgacattt
     3121 agcttcctac tggatagcac agtttaacaa aagtagtggt cacgtggatt atcatcactt
     3181 tgcttttccg gatgaaatta aaaattatgt ttcagtgagt gaagaagaaa aggctattaa
     3241 tgtgcctgct attatttatt ttgttgaaaa cggttcatgg ggagatatta ttttttatat
     3301 tttcaatgaa atgatttttc attccgaaaa aagcagagca ctagaaataa gtacatcaaa
     3361 tcacaatatg gcattaggct tgaagattaa agaaactaaa aatggggggg attttgtcat
     3421 tcagctttat gatcccaacc atacagcaac tcatttacga gcagagttta acaaatttaa
     3481 cttagctaaa ataaaaaaac tgactgtaga taattttctt gatgaaaaac atcagaaatg
     3541 ttatggtctt atatccgacg gtatgtctat atttgtggac agacatactc caacaagcat
     3601 gtcctccata atcagatggc ctgataattt acttcacccc aaagttattt atcacgcgat
     3661 gcgtatggga ttgactgagc taatccaaaa agtaacaaga gtcgtacaac tatctgacct
     3721 ttcagacaat acgttagaat tacttttggc agccaaaaat gacgatggtt tgtcaggatt
     3781 gcttttagct ttacaaaatg ggcattcaga tacaatctta gcatacggag aactcttgga
     3841 aacttctgga cttaaccttg ataaaacggt agaactacta actgcggaag gaatgggagg
     3901 acgaatatcg ggtttatccc aagcacttca aaatgggcat gcagaaacta tcaaaacata
     3961 cggaaggctt ctcaagaaga gagcaataaa tatcgaatac aataagctga aaaatttgct
     4021 gaccgcttat tattatgatg aagtacacag acagataccc ggactaatgt ttgctcttca
     4081 aaatggacat gcagatgcta tacgcgcata cggtgagctc attcttagcc cccctctcct
     4141 caactcagag gatattgtaa atttgctggc ctcaaggaga tatgacaatg ttcccggact
     4201 tctgttagca ttgaataatg gacaggctga tgcaatctta gcttatggtg atatcttgaa
     4261 tgaggcaaaa cttaacttgg ataaaaaagc agagctgtta gaagcgaaag attctaatgg
     4321 tttatctgga ttgtttgtag ccttgcataa tggatgtgta gaaacaatta ttgcttatgg
     4381 gaaaatactt cacactgcag accttactcc acatcaggca tcaaaattac tggcagcaga
     4441 aggcccaaat ggggtatctg gattaattat agcttttcaa aataggaatt ttgaggcaat
     4501 aaaaacttat atggaaataa taaaaaatga aaatattaca cctgaagaaa tagcagaaca
     4561 cttggacaaa aaaaatggaa gtgattttct agaaattatg aagaatataa aaagctgaat
     4621 attatctcgt ggtgataca
//
LOCUS       DAJTUP010000188         4632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTUP010000188 DAJTUP010000000
VERSION     DAJTUP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.191"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..301)
                     /gene="bamD"
                     /locus_tag="OO468_003898"
     CDS             complement(<1..301)
                     /gene="bamD"
                     /locus_tag="OO468_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR6260813.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNP"
     gene            436..1416
                     /gene="rluD"
                     /locus_tag="OO468_003899"
     CDS             436..1416
                     /gene="rluD"
                     /locus_tag="OO468_003899"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR6260814.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1413..2144
                     /gene="pgeF"
                     /locus_tag="OO468_003900"
     CDS             1413..2144
                     /gene="pgeF"
                     /locus_tag="OO468_003900"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR6260815.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2274..>4632
                     /gene="clpB"
                     /locus_tag="OO468_003901"
     CDS             2274..>4632
                     /gene="clpB"
                     /locus_tag="OO468_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR6260816.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRL"
BASE COUNT         1147 a         1117 c         1271 g         1097 t
ORIGIN      
        1 tcgggttaag gcgaataaaa cgatcgatgg cagcctgcgc taacggcaaa tcggcgtttt
       61 tatagtaggc gtagatgaga tccagctgca cctgctgcga atacggacca aacggatagc
      121 gattatctaa cgcttccagt tgcgttattg cctgtctcca gttaccgtcc tgcagctttt
      181 gttgtgcagt cgcgtaaatt tcatttggcg gattatcagg tacttcttcc tttgaccccg
      241 agcaacccgc caaaaacagg cttagtgtgg cggctgccac cagatatttc atgcgcgtca
      301 tgacgttttg actttcctca aaatgtaata cgggagattc tctgttcctg cacccggtta
      361 agaccagcta caatagcaca ctatattaaa cggcaaagcc gtaaaacccc aacgataaac
      421 gaagaagcag tatatatggc acaacgagta cagctcactg caacggtgtc cgaaaaccaa
      481 ctcggtcaac gcttagatca ggctttggcc gaaatgttcc cggattattc acgttcgcga
      541 ataaaagaat ggatcctcga ccagcgcgtg ctggtaaacg gcaaagtttg tgataagccg
      601 aaagaaaaag tattgggtgg cgagcaggtt gccatcaacg ctgagattga agaagaagcg
      661 cgttttgaac cgcaggatat ccctctggat atcgtctatg aagatgaaga tatcatcgtc
      721 attaataaac cgcgcgacct agtggtacat cctggcgcgg gtaacccgga tggcacggta
      781 ctgaatgcgt tgcttcatta ctatccgccc attgccgatg taccacgtgc gggcatcgtc
      841 catcgtctgg ataaagacac cactggcctg atggttgtgg caaaaaccgt tccggctcag
      901 acgcgtttag tcgaatcttt gcaacggcgt gaaattactc gtgagtatga agcggtggcg
      961 attggtcata tgaccgctgg cggtacagta gatgaaccca tcagccgcca cccgaccaaa
     1021 cgtactcaca tggcggtgca tccgatgggc aaaccggcgg tgactcacta tcgcatcatg
     1081 gaacacttcc gtgtgcacac gcgtctgcgg ttgcgtctgg aaactggacg tacgcaccag
     1141 atccgcgtgc atatggccca tatcactcat ccgctggtgg gcgatccggt ttatggtggc
     1201 cgtccgcgtc cgccaaaagg tgcttcggaa gcatttatct ccacgctgcg taagtttgac
     1261 cgccaggcgc ttcatgcaac catgctgcgt ctttatcacc cgatctccgg catcgaaatg
     1321 gaatggcatg cgcctattcc acaagatatg gtggagctga ttgaggtgat gcgcgccgat
     1381 ttcgaagagc ataaggatga agtggactgg ttatgagtaa gctgattgtc ccgcagtggc
     1441 cgctgccaaa aggtgttgcg gcctgtagct ccactcgtat cggcggcgtg agcttgcccc
     1501 cgtatgactc actcaacctc ggtgcccatt gtggcgataa cccggatcac gttgaggaga
     1561 atcgcaagcg actttttgct gcgggcaatt tgccttctaa accggtctgg cttgagcagg
     1621 tacacggcaa agatgtgctt aagcttactg gcgaacctta tgcctcaaaa cgagcagatg
     1681 cctcttatag caatacgcct ggcacggttt gcgcagtgat gactgccgac tgcctccctg
     1741 tgctgttttg caatcgagcg ggaacggaag tcgccgccgt tcatgctggc tggcgtggac
     1801 tgtgcgcagg cgtgctggaa gagacggttt cctgttttgc tgataaacct gaaaacattc
     1861 tcgcctggtt agggccggca attggtccac gcgcgttcga agtgggggcg gaggttcgcg
     1921 aggcgtttat ggcagtagac gctaaagcaa gtgcagcttt cattcagcat ggtgataagt
     1981 atctggcgga tatttatcag cttgcccggc agcgtctggc gaacgtgggt gttgagcaaa
     2041 ttttcggcgg cgaccgttgt acatatacgg aaaatgagac tttcttctct tatcgtcgcg
     2101 acaagaccac cggtcgtatg gcaagtttca tttggctgat ataacctaaa gaatcaagac
     2161 gatccggtac gcgtgatttt cttttcacat taatctggtc aataaccttg aataattgag
     2221 ggatgacctc atttaatctc cagtagcaac tttgatccgt tatgggagga gttatgcgtc
     2281 tggatcgtct tactaataaa ttccagcttg ctcttgccga tgcccaatca cttgcactcg
     2341 ggcacgacaa ccaattcatc gaaccacttc atttaatgag cgccctgctg aatcaggaag
     2401 ggggttcggt tagtccttta ttaacatccg ctggcataaa tgctggccag ttgcgcacag
     2461 atatcaatca ggcattaaat cgtttaccgc aggttgaagg tactggtggt gatgtccagc
     2521 catcacagga tctggtgcgc gttcttaatc tttgcgacaa gctggcgcaa aaacgtggtg
     2581 ataactttat ctcgtcagaa ctgttcgttc tggcggcact tgagtctcgc ggcacgctgg
     2641 ccgacatcct gaaagcaaca ggggcgacca ccgccaacat tactcaagcg attgaacaaa
     2701 tgcgtggagg tgaaagcgtg aacgatcaag gtgctgaaga ccaacgtcag gctttgaaaa
     2761 aatataccat cgaccttacc gaacgagccg aacagggcaa actcgatccg gtgattggtc
     2821 gtgatgaaga aattcgccgt accattcagg tgctgcaacg tcgtactaaa aataacccgg
     2881 tactgattgg tgaacccggc gtcggtaaaa ctgccatcgt tgaaggtctg gcgcagcgta
     2941 ttatcaacgg cgaagtgccg gaaggattga aaggccgccg cgtactggcg ctggatatgg
     3001 gcgcgctggt ggctggggcg aaatatcgcg gtgagtttga agaacgttta aaaggcgtgc
     3061 ttaacgatct tgccaaacag gaaggcaacg tcatcctgtt tatcgacgaa ttacatacca
     3121 tggtcggcgc gggtaaagcc gatggcgcaa tggacgccgg aaacatgctg aaaccggcgc
     3181 tggcgcgtgg tgaattgcac tgcgtaggtg ccacgacgct tgacgaatat cgccagtaca
     3241 tcgaaaaaga tgctgcgctg gaacgtcgtt tccagaaagt gtttgttgcc gagccttctg
     3301 ttgaagatac cattgcgatt ctgcgtggcc tgaaagaacg ttacgaattg caccaccatg
     3361 tgcaaattac tgacccggca attgttgcag cagcgacgtt gtctcatcgc tacattgctg
     3421 accgtcagct gccggataaa gccatcgacc tgatcgatga agcagcatcc agcattcgta
     3481 tgcagattga ctcaaaacca gaagaactcg accgactcga tcgtcgtatc atccagctca
     3541 aactggaaca acaggcgtta atgaaagagt ctgatgaagc cagtaaaaaa cgcctggata
     3601 tgctcaacga agaactgagc gacaaagaac gtcagtactc cgagttagaa gaagagtgga
     3661 aagcagagaa ggcatcgctt tctggtacgc agaccattaa agcggaactg gaacaggcga
     3721 aaatcgctat tgaacaggct cgccgtgtgg gggacctggc gcggatgtct gaactgcaat
     3781 acggcaaaat cccggaactg gaaaagcaac tggaagccgc aacgcagctc gaaggcaaaa
     3841 ctatgcgtct gttgcgtaat aaagtgaccg atgccgaaat tgctgaagtg ctggcgcgtt
     3901 ggacggggat tccggtttct cgcatgatgg aaagcgagcg cgaaaaactg ctgcgtatgg
     3961 agcaagaact gcaccatcgc gtcattggtc agaacgaagc ggttgatgcg gtatctaacg
     4021 ctattcgccg tagccgtgcg gggttggcgg atccaaatcg cccgattggt tcattcctgt
     4081 tcctcggccc aacgggtgtg gggaaaactg agctttgtaa ggcgctggcg aactttatgt
     4141 ttgatagcga cgaggcgatg gtccgtatcg atatgtccga atttatggag aaacactcgg
     4201 tgtctcgtct ggttggtgcg cctccgggat atgtcggtta tgaagaaggt ggctacctga
     4261 ccgaagctgt gcgtcgtcgt ccgtattccg tcatcctgct ggatgaagtg gaaaaagcgc
     4321 atccggatgt cttcaatatt ctgttgcagg tactggatga tgggcgtctg actgacgggc
     4381 aggggagaac ggtcgacttc cgtaatacgg ttgtcattat gacctctaac ctcggttccg
     4441 atctgattca ggaacgcttc ggtgaactgg attatgcgca catgaaagag ctggtgctcg
     4501 gtgtggttag ccataacttc cgtccggaat tcattaaccg tattgatgaa gtggtggtct
     4561 tccatccgct gggtgaacag cacattgcct cgattgcgca gattcagttg aaacgtctgt
     4621 acaaacgtct gg
//
LOCUS       DAJTUP010000189         4615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTUP010000189 DAJTUP010000000
VERSION     DAJTUP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.192"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..292)
                     /locus_tag="OO468_003902"
     CDS             complement(<1..292)
                     /locus_tag="OO468_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260817.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLI"
     gene            complement(360..2309)
                     /locus_tag="OO468_003903"
     CDS             complement(360..2309)
                     /locus_tag="OO468_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR6260818.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2394..2729)
                     /locus_tag="OO468_003904"
     CDS             complement(2394..2729)
                     /locus_tag="OO468_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR6260819.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2729..3085)
                     /locus_tag="OO468_003905"
     CDS             complement(2729..3085)
                     /locus_tag="OO468_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR6260820.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3085..4581)
                     /locus_tag="OO468_003906"
     CDS             complement(3085..4581)
                     /locus_tag="OO468_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR6260821.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVEKYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1078 a         1358 c         1009 g         1170 t
ORIGIN      
        1 ggatcaaaaa tgtcatcgtc gctggtgatg cattgtttgc aaaatcaaat acacctatac
       61 ttttgccgaa ttcataagaa gcttttctca atataatcgc accgctggca gaaggggatg
      121 ggcacgatat cctcagttgt ctgaatccat ctgtatccaa gtcaacgtca ttatctgcag
      181 atgttttaac gttactaata aatcgtgaaa caatctcctc atttgttagt attttcgcct
      241 gagcacagca agaggttaat attgcaaccg tcattaaaaa tggctttatc acaaaacaaa
      301 ctccattgtt agcatgttac gcgataattt tgcctcatct ttcaccacaa aaaaagaact
      361 caccttttaa atggagaata cccaacatca tgggtgattt tcataaaggg atcgcctgtt
      421 ttcggcaaat cgataacgcg cattccaggc ggcgcgttgt caaacgtcac cttaagttcg
      481 ctgcgggttg ccggtgaaga tgcaaggctt aacagctgat tcctttccag tgatacattc
      541 ggggtgtagc gtttatcggg agataaatac ccatcaccac gtggcatctg ccatccggtc
      601 atgtcataga caaaatcatg gaactgtttg ccccactcgt cgaacttatc attcagacca
      661 aatccactgt tcagtgcatc agccacaaaa tttttaacaa gccagggatg ttctttttca
      721 aactcctgcg ccatcatgcc cagttcaagc aatccaccaa tcagactgat ctttccgagg
      781 ccctttaatt tcagccctgt ttttcctgcc gccgttgtcc attgattctg ggcgactgtt
      841 gccgcacgaa tacttgccac cgccttcaca ccaatataca gggcagaaac cgttgctatt
      901 gtttttactg cctgttccca tccccccatt gctctgacaa cctgatcaac ctcctgccag
      961 acttttttca caaccggacc gaccgtttcc cagttatcaa ttatgagata tgcaccaccg
     1021 acgagaagcg caattaatcc ctttgccggc gtcatattca tcacgctacc catgattttt
     1081 gttatgcgtg tcaatgtgcc aatcgccacg cccatcgtca gtaatgccgc accagttttc
     1141 gcgatggttt ttacgacctc aggattttct ctgacaaacg tgcgcacttc ttccagaaaa
     1201 ggcttcatct cttttatgcc ttcattgagt gaaggcagga aggtttcccc cagcgtggaa
     1261 gaaatcgcat tgatctggtt ctgcagcagc aacaactgat tttctgtcgt cgcggcacgg
     1321 gcggcatatt ctttctgcat tgagccgcca tactgctgcg catccgccac acgattaaag
     1381 ttggttcgca acaaatccag attcgtgagc agcggcgcaa tggcccccag ggattccttc
     1441 ccgaacagcg ctttcagcac agcagcctgt ttttctttcg gcacttttgc cagagaatcc
     1501 agtacgtgca gcatggcccc gcgggcgtct ttctgcatat ccgccgccag ttttttcggg
     1561 ctaatgcgca acgagcggag cacttttttc tgcgatttgg tggcagaatc cctcgctgtc
     1621 agcgacaaca taaaattctt tatccccgtc gcagcaattt ctgactccac ccccattccg
     1681 gcaatggttg cccccattgc ggcaatctct ccggaggcca cacccgcaac actgcctaaa
     1741 gggccaatac gggtcacaac atcagaaatc tttttcgcac ttgcaggacc ggtattgcca
     1801 agatagttaa tcttatccgc aagccctgcc acctctccct gtgtcagttt aaaggcagtg
     1861 cgccactgcg ccatcatctg accggattct tctgccgtgg tgtcaaaggc cacgcccatc
     1921 ttcacggcat cgtcagtaaa ctgcatcagc tcgtcacggg cgataccagc ctgaccaccc
     1981 gccgccacaa tttcggcgat accttccgcc gacatgggca gttctgttga caggtcgcgc
     2041 acctgctccg tcattgcctt aaacgcttcc ggcgtatcca gaccatccac cactttccgg
     2101 acatccgcca tttttgattc aagggcaatg gcagatttga ccgggagcgc cagcgccccc
     2161 agtactgcgg ttccggcacc cgctgcgccc agtgacaggc tggcaaactc ctttttaaaa
     2221 cctttcagct ggcgctgcat tcccttcagc ggtgccgata ccttatccac ggcagtgatg
     2281 atcgccttca gctgaaaact gtcagccatg cttcatctcc tcgttaatgc ggacggcctc
     2341 ggcttccagt tctgtaaact gcgaaacagc cacccgtcgc agctccagtg gattcagttt
     2401 ccagaaccag gcaacattgt agagtcgttt ccggaggtgc ttcccgtctc cgactgggta
     2461 aaaaaacgca ggatctgcat gctggtctta aaaatatcca gttttgccat ctgcgccgcc
     2521 gatgaacacg ggatccccgc cagcaacggg atatatttca gtgccacctg gctgtccagt
     2581 ttaatactgc cttcaccgga aataatgaaa ggaaaaccca gcgcctcgat ttcgtcatac
     2641 gtgggctcgc gcaactccag cacatgcagc atttcgttat gcgctgtcac cggttttttt
     2701 aaaactattt ctgtaacgtt cattgccatt actgataccc tccctcttcg ccgtggaatt
     2761 caagatctgc cgtgccttct tcggcattat gattagcctc tccgtgcagc catgccgccg
     2821 aaagcacata caccatgccg tttgccagct cagcggtaat cgtcatctgg tctgaggtgg
     2881 taattttgtc gaccggaaag tttttgggca ctttaaacgt gcccttaacg taaggtgacc
     2941 gccaggtctc cttgtaatcc acatcccctg ccatgccgac gatgtcatcc cggacattgg
     3001 tgttcatcgg cacttcaatg ccacctgtca gcgataactg ctgaccatcc actttaaaaa
     3061 aacaggttcc ggcgatcttt gccattatgc tgactcctct gaatactgaa gacggaactg
     3121 gttaaccacc gcaaagacac gtaactgatt aacataatcc ggcgggaaca gcgtattcag
     3181 tcggttcggg ttatctgcat cacgctcaac tatcagatac tgtttaaaca gatcgtattt
     3241 ttccacaata ccggcacgct ccatctgacg atatgtcgcc agaagttccc ctttgataac
     3301 ggcaggagtg acaatcgcct gccccggacc aaaacgggta ccatcatttg ccagcttgtg
     3361 acgtccgtac ttgcttgtga tgaccgattt cagtttgcga agaacatatg cactggtatg
     3421 cagagtttca ctgtccagat agctgttgtc tgccacgcca tacgcattct ttttgtaggt
     3481 ggttacggaa cgctggatct gcaacgtgcc gccttccaca taagccgtcg ccacgccgtg
     3541 agataaaagg gtctgctgct ctgtcatgat gaatcgctta cctttcggtg ccggaagcat
     3601 ccccaccagc tcccccgtct gtgtcggacg ggcaggatca ttccggataa ataccgcttc
     3661 acgggcaagg cgactggcaa ccagttcatc gacaggcgac tgggtttctt tttcgtaacc
     3721 ggcaagcgtg atatgttgct gattatgcat atctccggca ccaaccagct ctgacagcgt
     3781 tcccagtttt gcggtataga catgcccgta taactggcgc gcataactcc agcgaccgct
     3841 gctgtcattc atttcggtca tcatcatatt gatggaggcg gcatcgttga acggcagacc
     3901 gataaagtcg aatgcctcat cgcccatagc agcaacagcg gcggtaagat caggcgctcc
     3961 gctaccggca gcaccgactt ccgtcaccac ccgaagccct gctggcaaaa tctcaccacc
     4021 accagaacca taataattca gacagaccgg taactcatta ccatacagcc ccttatgacg
     4081 ggcagtcagc gtcaccacgc ctgcatcaga tgacgccgta aacggcaggg tgataacccc
     4141 gtttaccgct tccttaatcg cggtggcaac cgcagtggca tcatcgccat tcaccacagg
     4201 cacctgtaca cgggagcgcc cgacatacag actcagggtg ccgctttcct ctgcttctcc
     4261 ggcaaccgtc accctgaccg tcgccgccgc ccctctggct tccggtactg caataacata
     4321 cagttcaccg aaaggatctg tctgacggta gacttccacc atacgcgcca gctggctccc
     4381 cgccccacaa atctggcggg cataatctgc cgatggcatc agcaccaggc tgttaacctc
     4441 aatggctgca tcgttgctgg catgcccgat cagtaatgca ggcgcgctgg tcaccgctgt
     4501 atttgccgca gaattgttca tctcggcgta gaacaacggc accagcgtat tcgacggaac
     4561 agtattaaaa cttatcgtca tgatttttta gccttatcct gtttaacacg tacca
//
LOCUS       DAJTUP010000190         4579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTUP010000190 DAJTUP010000000
VERSION     DAJTUP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.193"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..369
                     /locus_tag="OO468_003907"
     CDS             <1..369
                     /locus_tag="OO468_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR6260822.1"
                     /translation="RAFPRHIPAQNAVLDVTFEFPRGRATKDRLYFCVPMLDLVVPEE
                     GELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLLCYGHCKTVDEAIIFIRAR
                     RSHIVLKEEHKAMLKLWENR"
     gene            complement(423..1028)
                     /gene="azoR"
                     /locus_tag="OO468_003908"
     CDS             complement(423..1028)
                     /gene="azoR"
                     /locus_tag="OO468_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR6260823.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1229..>4579
                     /gene="hrpA"
                     /locus_tag="OO468_003909"
     CDS             1229..>4579
                     /gene="hrpA"
                     /locus_tag="OO468_003909"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR6260824.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGV"
BASE COUNT         1127 a         1160 c         1259 g         1033 t
ORIGIN      
        1 agggcgtttc cacgacatat tccggcacag aatgcggttc tggacgtcac ctttgaattc
       61 cctcgtggac gagcgacaaa agatcgactc tatttctgtg taccgatgtt ggatctggtg
      121 gttccggaag agggggagct ccgacaggcc gtggcgatgc tggaaacatt acgcgaagag
      181 caaggcagcg ttctggtcca ttgcgcattg ggattatcgc gcagtgcgct ggtagtggcg
      241 gcatggctgt tatgttacgg acactgtaaa acagttgatg aagcgattat ctttattcga
      301 gccagacgct cgcatattgt gcttaaggaa gagcacaaag cgatgttgaa attatgggaa
      361 aacaggtaag tggattgaga tgtggactga atatctacag tccacatcaa gaccgtgtcc
      421 ggttatgcag caacaatgct gtcgatggct gcttttgcgt cagactgtgc tttcgctgcc
      481 atttccggac cgtatgcgat cccttcggcg aagacaaatt tcacatcggt aatgccgata
      541 aagccgagga acgtggacag atacggcgtc accaggtccg ttggtccatc tttgtggatc
      601 ccaccgcggc tggtaataac gatggctttt ttacccgtta ccagaccttc cggaccgttc
      661 tcggtatagc ggaaagtaac gcctgcgcgt gcaaccaggt caaaatagtt tttcaactga
      721 gttgagatgt tgaagttata catcggtgcc gcaataacga taacgtcgtg ggctttcagc
      781 tcggcaatca gctcatcgga aagcgccaga gcttcctgct gacgcggagt cagcggcgca
      841 tcgctcggac gcagagcgcc aaccagttcg ccatccagta ccggaatcgg atttgcagct
      901 aggtcgcgaa cggtgatttc atcagcggag tgcttttcgc tccattgttc aacaaaataa
      961 tcggacaact gattagactg agagtaccct gccaggatgc tggatttaag aactaatacc
     1021 ttgctcatgg tgtttcctta tagatgtttg aatggacgat gccccgttgc ttgctgacac
     1081 tttattcaca atcctgccac agagatcgcg caataaatcg aagtctatgt tcgaatttat
     1141 tgaacaacgc atagaaagcc gcgatgtggt actctatatc tatcatttaa aagaaaatta
     1201 atcaggcaga ctactgccca ctaacgttat gacagaacaa caaaaattga cctttactgc
     1261 cttgcaacag cggctggatt cgctgatgct gcgcgacaga ctgcgttttt ctcgccgtct
     1321 gcattgtgtt aagaaggtta aaaatcctga tgcacaacag gccattttcc aggagatggc
     1381 gaaagagatt gaccaggcgg cagggaaagt cctgctgcgt gaagcggcac gaccggaaat
     1441 cacttatcct gacaatttac cggttagtca gaaaaaacag gacatactcg aagcgattcg
     1501 tgatcaccag gtggtgatcg tcgccgggga aaccggctcc ggtaaaacga ctcagctacc
     1561 gaaaatctgt atggagctgg ggcgcggtat taaagggctg atcggtcata cccagccgcg
     1621 tcgcctggcg gcacgaacgg tggccaaccg tattgcggaa gagctgaaaa cggagccggg
     1681 cggttgcatc ggttacaaag tgcgtttcag cgatcacgta agtgataaca cgatggtcaa
     1741 gctgatgacc gacgggatcc tgctggcgga gatccagcaa gaccgcctgc tgatgcagta
     1801 cgacaccatc attattgacg aagcgcacga acgcagcctg aatatcgatt ttctgctcgg
     1861 ctatttgaaa gagttgctgc cgcggcgtcc tgacctaaaa atcattatca cttccgcgac
     1921 tatcgacccg gaacgctttt cgcgccactt taataatgcg ccgattattg aagtctccgg
     1981 tcggacctat ccggtggaag tgcgctatcg cccgattgtt gaagaagccg atgacaccga
     2041 gcgcgatcag ttgcaggcga tttttgacgc cgtagacgaa ctaagccagg aaagccccgg
     2101 cgacattctg atctttatga gcggcgagcg ggaaattcgc gataccgccg atgcgctgaa
     2161 caagctgaac ttacgccata ccgaaatctt gccgctttat gcgcggcttt cgaacagcga
     2221 acagaaccgc gtgttccagt cgcacagcgg acggcgcatt gtgctggcga ccaacgtcgc
     2281 ggaaacctcg ctgaccgtac cggggattaa atacgtcatc gaccccggta cagcgcgtat
     2341 cagccgctac agctatcgca ccaaagtgca gcgtttgccg attgaaccca tttcgcaggc
     2401 gtctgccaat cagcgtaaag gccgctgtgg tcgtgtgtcc gaagggatct gtattcgtct
     2461 ctattccgaa gacgatttcc tctcgcgccc ggagtttacc gatccggaga ttctgcgtac
     2521 caacctggcc tcggttattt tgcagatgac cgcgctgggg ctgggcgata tcgctgcgtt
     2581 cccgtttgtc gaagcaccgg ataaacgcaa tatccaggat ggcgtgcgtc tgctcgaaga
     2641 actgggcgcg atcaccacgg atgaacaggc cagcgcctat aaactgacgc cgctcggtcg
     2701 ccagctctcg cagttgcctg tcgacccacg tctggcgcgt atggtgctgg aagcgcaaaa
     2761 acatggctgc gtgcgtgagg cgatgattat cacgtccgcg ctctccatcc aggatccgcg
     2821 cgaacgtccg atggacaaac agcaggcatc ggacgaaaaa catcgtcgct tccacgacaa
     2881 agaatccgac ttcctcgcgt ttgtaaatct gtggaattat cttggcgagc agcaaaaggc
     2941 gctttcttcc aacgccttcc gtcgcctgtg tcgtaccgat tacctcaact atctgcgcgt
     3001 gcgcgaatgg caggatatct acacccagtt gcgccaggtg gtgaaagaac ttggcattcc
     3061 ggtcaacagc gaaccggcgg agtatcgtga aattcacatc gccttactga ccggtttgct
     3121 ttcccatatc ggcatgaaag atgccgataa acaggaatat accggtgcac gtaacgcgcg
     3181 tttctccatc ttccccggtt ctggtttatt caaaaagccg ccgaaatggg taatggtggc
     3241 ggaactggta gaaaccagcc gcctgtgggg gcgcattgct gcgcgtatcg acccggaatg
     3301 ggtagaaccc gttgctcagc atttgattaa acgcacctac agcgaaccgc actgggaacg
     3361 ggcgcagggc gcggtgatgg caacggaaaa agtcactgtt tatggtttgc cgattgttgc
     3421 tgcgcgcaag gtcaactaca gccagatcga ttcggcgtta tgtcgtgaac tctttattcg
     3481 ccacgctctg gtggaaggtg actggcagac gcgtcacgca ttcttccgtg aaaacctgaa
     3541 actgcgggcc gaagtggaag agctggaaca caaatcacgt cgccgcgata ttctggttga
     3601 tgacgaaacg ctgtttgagt tctacgacca gcgcatcagc cacgatgtaa tctccgctcg
     3661 tcacttcgat agctggtgga aaaaagtcag ccgcgaaacg cctgatttgc tcaactttga
     3721 aaagagcatg ttgatcaaag aaggggcgga aaaaatcagc aagctggatt acccgaactt
     3781 ctggcatcag ggcaatctca agctgcgttt gagatatcag tttgagccag gcgcggacgc
     3841 ggacggtgtg accgtacata ttccgctgcc gttacttaac caggttgagg aaagcgggtt
     3901 tgaatggcag atccccggtc tgcgccgcga attggtgatt gctctgatta aatcgttgcc
     3961 gaaaccggta cgccgtaatt ttgtgcctgc accaaactac gccgaagcgt ttttaggccg
     4021 cgtcacaccg ctggagttac cgttgctcga cagccttgag cgcgagttac ggcggatgac
     4081 cggcgttacc gttgaccgcg aagactggca ctgggatcag gtgcccgatc acctgaaaat
     4141 caccttccgc gtggtggatg acaaaaacaa gaagctaaaa gaagggcgct cgctacaaga
     4201 tctgaaagat gcgctgaaag gcaaagtgca ggaaacgctg tccgcggtgg cggatgacgg
     4261 tatcgagcag agcggcttac atatctggag ttttggtcag ctgccggaaa gttacgaaca
     4321 gaagcgtggc aactataaag tgaaggcgtg gccggcgctg gtggatgaac gcgacagcgt
     4381 gacgatcaaa ctgtttgata acccgctgga gcaaaaacag gcaatgtgga atggtcttcg
     4441 ccgtctactg ctgctaaata tttcctcgcc gatcaagtat ttacatgaga agttaccaaa
     4501 caaagccaag ctggggctgt actttaaccc gtatggcaaa gtgctggagc tgatcgacga
     4561 ctgtatctcc tgcggtgtg
//
LOCUS       DAJTUP010000191         4470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTUP010000191 DAJTUP010000000
VERSION     DAJTUP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.194"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            80..2071
                     /gene="cyoB"
                     /locus_tag="OO468_003910"
                     /pseudo
     CDS             80..2071
                     /gene="cyoB"
                     /locus_tag="OO468_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2061..2675
                     /locus_tag="OO468_003911"
     CDS             2061..2675
                     /locus_tag="OO468_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR6260825.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            2675..3004
                     /locus_tag="OO468_003912"
     CDS             2675..3004
                     /locus_tag="OO468_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR6260826.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3016..3906
                     /gene="cyoE"
                     /locus_tag="OO468_003913"
     CDS             3016..3906
                     /gene="cyoE"
                     /locus_tag="OO468_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR6260827.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4090..4401
                     /locus_tag="OO468_003914"
     CDS             4090..4401
                     /locus_tag="OO468_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6260828.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
BASE COUNT          946 a         1117 c         1198 g         1209 t
ORIGIN      
        1 cagtgcacac gaaggtatgg aaggcatgga catgagccac gcggaatccg cccattaaag
       61 gggttgagga agaataaaga tgttcggaaa attatcactt gatgcagtcc cgttccatga
      121 acctatcgtc atggttacga tcgctggcat tattttggga ggtctggcgc tcgttggcct
      181 gatcacttac ttcggtaagt ggacctacct gtggaaagag tggctgacct ccgtcgacca
      241 taaacgcctc ggtatcatgt atatcatcgt ggcgattgtg atgttgctgc gtggttttgc
      301 tgacgccatt atgatgcgta gccagcaggc tcttgcctcg gcgggtgaag cgggcttcct
      361 gccacctcac cactacgatc agatcttcac cgcgcacggc gtgattatga tcttcttcgt
      421 ggcgatgcct ttcgttatcg gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg
      481 tgacgttgcg ttcccgttcc tcaacaactt aagcttctgg tttactgttg ttggcgtgat
      541 tctggttaac gtttctctcg gcgtgggcga atttgcgcag accggctggc tggcctatcc
      601 accgctatcg ggaatagagt acagtccggg agtcggtgtt gattactgga tatggagtct
      661 ccagctatcc ggtataggta cgacgcttac cggtatcaac ttcttcgtta ccattctgaa
      721 gatgcgcgca ccgggcatga ccatgttcaa gataccagta tttacctagg catcactgtg
      781 cgcgaacgta ctgattattg cttccttccc aattctgacg gttaccgtcg cgttgttgac
      841 cctggatcgc tatctgggca cccatttctt taccaacgat atgggtggca acatgatgat
      901 gtacatcaac ctgatttggg cctggggcca cccggaagtt tacatcctga tcctgcctgt
      961 tttcggtgtg ttctccgaaa ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac
     1021 ctcgctggta tgggcaaccg tctgcatcac cgtgctgtcg ttcatcgttt ggctgcacca
     1081 cttctttacg atgggtgcgg gcgcgaacgt aaacgccttc tttggtatca ccacaatgat
     1141 tatcgccatc ccgaccgggg tgaagatctt caactggctg ttcaccatgt atcagggccg
     1201 catcgtgttc cattctgcga tgctgtggac tatcggtttt atcgtcacct tctcggtggg
     1261 cggtatgact ggcgtgctgc tggcagtacc gggcgcggac ttcgttctgc ataacagcct
     1321 gttcctgatt gcgcacttcc ataacgtgat catcggcggc gtggtcttcg gctgcttcgc
     1381 agggatgacc tactggtggc ctaaagcgtt cggtttcaaa ctgaacgaaa cctggggtaa
     1441 acgcgcgttc tggttctgga tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct
     1501 gggcttcatg ggcatgaccc gtcgtttgag ccagcagatt gacccgcagt tccacaccat
     1561 gctgatgatt gcagccagcg gtgcagtact gattgcgctg ggtattctct gcctcgttat
     1621 tcagatgtac gtttctattc gcgaccgcga ccagaaccgt gacctgactg gcgacccgtg
     1681 gggtggccgt acgctggagt gggcaacctc ttccccgcct ccgttctata actttgccgt
     1741 agtgccgcac gttcacgaac gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta
     1801 taaaaagcct gaccactatg aagaaattca tatgccgaaa aacagcggtg caggtatcgt
     1861 cattgcagct ttctccacca tcttcggttt cgccatgatc tggcatatct ggtggctggc
     1921 gattgttggc ttcgcaggca tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt
     1981 ggattactac gtgccggtgg cagaaatcga aaaactggaa aaccagcatt tcgatgagat
     2041 tactaaggca gggctgaaaa atggcaactg atactttgac gcacgcgact gcccacgcgc
     2101 acgaacacgg gcaccacgat gcaggcggaa ccaaaatctt cggattttgg atctacctga
     2161 tgagcgactg cattctgttc tcaatcttgt ttgctaccta tgccgttctg gtgaacggca
     2221 ccgcaggcgg cccgacaggt aaggacattt tcgaactgcc gttcgttctg gttgaaactt
     2281 tcttgctgtt gttcagctcc atcacctacg gcatggcggc tatcgccatg tacaaaaaca
     2341 acaaaagcca ggttatctcc tggctggcgt tgacctggtt gtttggtgcc ggatttatcg
     2401 ggatggaaat ctatgaattc catcacctga ttgttaacgg catggggccg gatcgcagcg
     2461 gcttcctgtc agcgttcttt gcgctggtcg gcacgcacgg tctgcacgtc acttctggtc
     2521 ttatctggat ggcggtgctg atggtgcaaa tcgcccgtcg cggcctgacc agcactaacc
     2581 gtacccgcat catgtgcctg agcctgttct ggcacttcct ggatgtggtt tggatctgtg
     2641 tgttcactgt tgtttatctg atgggggcga tgtaatgagt cattctaccg atcacagcgg
     2701 cgcgtcccat ggcagcgtaa aaacctacat gacaggcttt atcctgtcga tcattctgac
     2761 ggtgattccg ttctggatgg tgatgacagg ggctgcctct ccggccgtaa ttctgggaac
     2821 aatcctggca atggcagtgg tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa
     2881 taccaaatca gatgaaggct ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc
     2941 tatcctggtt gtaggctcca tctggattat gtggaacctc aactacaaca tgatgatgca
     3001 ctaagagcgg cggttatgat gtttaagcaa tacctgcaag taacgaaacc aggcatcatc
     3061 tttggcaacc tgatctcggt gattggggga ttcctgctgg cctcaaaggg cagcattgat
     3121 tatcccctgt ttatctacac gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg
     3181 tttaacaact acatcgacag ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg
     3241 ctggttaaag gcctgatctc tcctgctgtc tcgctggtgt acgccacctt gctgggtatt
     3301 gctggcttta tgctgctgtg gtttggcgcg aatccgctgg cctgctggct gggggtgatg
     3361 ggctttgtgg tttatgtcgg cgtctatagc ctgtacatga aacgccactc agtctacggc
     3421 acattgattg gttcgctctc cggcgctgcg ccgtcggtga ttggctactg tgcagtaacc
     3481 ggcgagtttg atagcggcgc agcgatcctg ctggctatct tcagcctgtg gcagatgcct
     3541 cactcctatg ccatcgccat tttccgcttt aaggattacc aggcggcaaa cattccggta
     3601 ttgccagtgg taaaaggcat ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc
     3661 tttgccgttg ccacgctgat gctctctctt ggcggttacg ctgggtataa atatctggtg
     3721 gtcgccgcgg cggttagcgt ctggtggtta ggtatggctc tgcgcggtta taaagttgct
     3781 gatgacagaa tctgggcgcg caagctgttc ggcttctcta tcatcgccat cactgccctc
     3841 tcggtgatga tgtccgttga ttttatggta ccggactcgc atacgctgct ggctgctgtg
     3901 tggtaacaaa acctctctat taaaaaggtg ctacggcacc tttttattgt ctgagatcag
     3961 agatacggct aataaccgta tctcttgaca gagtgagcag atgcacataa attaatcata
     4021 cggttgatag ccgtattatg tatgagatcg actccctacg atttcattca gttaatcaaa
     4081 gaagtcataa tgcctggaaa aacagccgct ctatacgatg tcgataaaac gctgaaaaat
     4141 gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca aacttcgtga agctgcgcaa
     4201 gccgttgggg tagatttaag cgcttttatt ttgagcgctg ctatggaacg cgccgaaagt
     4261 gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa gctgggaact gatgaaccaa
     4321 ctcatcgctg aacctgctca accgacgctc gccctcaagg cgttaatgaa aaggaaaaac
     4381 agcgatggtc gacaagcatg aagagattac tctgcccata gtcctctcct gtaattatca
     4441 gtctgatatt acttatcctg ggcaaaaaca
//
LOCUS       DAJTUP010000192         4451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTUP010000192 DAJTUP010000000
VERSION     DAJTUP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.195"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..586
                     /gene="tauA"
                     /locus_tag="OO468_003915"
     CDS             <1..586
                     /gene="tauA"
                     /locus_tag="OO468_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR6260829.1"
                     /translation="VPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDI
                     DGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSA
                     IDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPV
                     NKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            599..1366
                     /gene="tauB"
                     /locus_tag="OO468_003916"
     CDS             599..1366
                     /gene="tauB"
                     /locus_tag="OO468_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR6260830.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1363..2190
                     /gene="tauC"
                     /locus_tag="OO468_003917"
     CDS             1363..2190
                     /gene="tauC"
                     /locus_tag="OO468_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR6260831.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2187..3038
                     /gene="tauD"
                     /locus_tag="OO468_003918"
     CDS             2187..3038
                     /gene="tauD"
                     /locus_tag="OO468_003918"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR6260832.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3144..4118)
                     /gene="hemB"
                     /locus_tag="OO468_003919"
     CDS             complement(3144..4118)
                     /gene="hemB"
                     /locus_tag="OO468_003919"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR6260833.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
BASE COUNT          995 a         1127 c         1279 g         1050 t
ORIGIN      
        1 cgtaccgttt atctccacca cccactacag cctgctggcg gcgctgaaac actgggggat
       61 taaaccaggg ctagtggaga ttgtgaacct gcagccgccc gcgattatcg ccgcatggca
      121 gcggggtgat attgatggtg cttatgtctg ggcaccggcg gttaacgccc tggaaaaaga
      181 cggcaaggtg ctgaccgatt ctgaacaggt cgggcagtgg ggtgcgccaa cgctggacgt
      241 ctgggtggtg cgcaaagatt ttgccgagaa acatcctgag gtcgtgaaag cgttcgccaa
      301 aagcgccatc gatgcacagc aaccgtacat tgctaaccct gacgcgtggc tgaaacagcc
      361 ggaaaacatc agcaaactgg cgcgtttaag cggcgtgcct gaaggtgacg ttccggggct
      421 ggtgaaggga aatacctatc tgacgccgca gcaacaaacg gcagaactga ccggaccggt
      481 gaacaaagca atcatcgaca ccgcgcagtt tttgaaagag cagggcaagg tcccggctgt
      541 agcgaatgat tacagccagt acgttacctc gcgcttcgtg caataaaagg aggcgcagat
      601 gctgcaaatc tctcatctct atgccgatta tggcggcaaa ccggcactgg aagatatcaa
      661 cctgacgctg gaaagcggcg agctactggt ggtgctgggg ccgtccggct gcggtaaaac
      721 caccctgctg aatctgattg ccggttttgt gccttatcag catggcagca ttcaactggc
      781 gggtaagcgt attgagggac cgggagcaga gcgtggcgta gtttttcaga atgaagggct
      841 gctaccgtgg cgcaatgtac aggacaacgt ggcgttcggc ctgcaattgg caggtataga
      901 gaaaatgcag cgactggaaa tcgcgcacca gatgctgaaa aaagtggggc tggaaggcgc
      961 agaaaaacgc tatatctggc agctttccga tggtcaacgt cagcgggtgg ggattgcccg
     1021 cgcgctggcg gcgaatcccc agctgttatt actcgacgaa ccgtttggtg cgctggacgc
     1081 cttcacccgc gaccagatgc aaactctgct gctgaaactc tggcaggaga cgggcaagca
     1141 ggtgctgttg attacccacg atatagaaga agcggtgttt atggcgactg aactggttct
     1201 gctttcatcc ggccctggcc gtgtgctgga gcggctgcgg ctcaactttg ctcgccgctt
     1261 tgttgcggga gagtcgagcc gcagcatcaa gtccgatcca caattcatcg ccatgcgcga
     1321 atatgtttta agccgcgtat ttgagcaacg ggaggcgttc tcatgagtgt gctcattaat
     1381 gaaaaactgc attcgcggcg gctgaaatgg cgctggccgc tctcgcgtca ggtgacctta
     1441 agcattggca cgttagcggt tttactcact gtatggtgga cggtggcggc gctgcaactg
     1501 attagcccgc tgtttttgcc gccgccgcaa caagtactgg caaaactgct caccattgcc
     1561 ggaccgcagg gatttatgga cgccacgctg tggcagcatc tggcagccag cctgacgcgc
     1621 attgtgctgg cgctactcgc ggcggtggtg atcggtattc cggtcgggat cgccatgggg
     1681 ctaagcccga cggtgcgcgg cattctggat ccggtaatcg aactttatcg tccggtgcca
     1741 ccgctggctt atttgccgct gatggtgatc tggtttggta ttggtgaaac ctcgaagatc
     1801 ttactgatct atttagcgat ttttgcgccg gtggcgatgt cggcgctggc aggggtgaaa
     1861 agcgcgcagc aggttcgcat tcgtgccgcc cagtcgctgg gtgccagccg tgcgcaggtg
     1921 ctgtggtttg tcattttgcc cggcgcgctg ccggaaatcc tcaccggatt acgcattggt
     1981 ctgggtgtgg gctggtctac gctggtagcg gcggagctga ttgccgcgac acgcggttta
     2041 ggatttatgg ttcagtcagc gggtgaattt ctcgcgactg acgtggtact ggcggggatc
     2101 gcggtgattg cgattatcgc ctttctttta gaactgggtc tgcgcgcgtt acagcgccgt
     2161 ctgacgccct ggcatggaga agtacaatga gtgaacgtct gagcattacc ccgctggggc
     2221 cgtatatcgg cgcacaaatt tcgggtgccg acctgacgcg cccgttaagc gataatcagt
     2281 ttgaacagct ttaccatgcg gtgctgcgcc atcaggtggt atttctgcgc gatcaagcta
     2341 ttacgccgca gcagcaacgc gcgctggccc agcgttttgg cgaactgcat attcaccctg
     2401 tttaccctca tgccgaaggg gttgacgaga tcatcgtgct ggatacccat aacgataatc
     2461 cgccggataa cgacaactgg cataccgatg tgacatttat tgaaacgcca cccgcagggg
     2521 cgattctggc agctaaagag ttaccttcga ccggcggtga tacgctctgg accagcggta
     2581 ttgcggccta tgaggcgctc tctgttccct tccgccagct gctgagctgg ctgcgtgcgg
     2641 agcatgattt ccgtaaatca ttcccggaat acaaataccg caaaaccgag gaggaacatc
     2701 aacgctggcg cgaggcggtc gcgaaaaatc ctccgttgct acatccggtg gtgcgaacgc
     2761 atccggtgag cggtaaacag gcgctgtttg tgaatgaagg ctttactacg cgaattgtcg
     2821 atgtcagcga gaaagagagc gaagccttgt taggcttttt attcgcccat atcactaaac
     2881 cggagtttca ggtgcgttgg cgctggcaac cgaatgatat tgcgatttgg gataaccgcg
     2941 tgactcagca ctatgccaat gccgattacc tgccacagcg gcggattatg catcgggcga
     3001 cgatccttgg ggataaaccg ttttatcggg cggggtgata cgagtgcgga cggttccctc
     3061 gcccccttgg ggagagggtt agggtgaggg ggcgttcact gtactttcaa caggttaact
     3121 ccccctttct gagaggaaaa aaattaacgc agaatcttct tctcagccaa atccagcgca
     3181 aagtagctga aaatcagatc cgcacccgca cgcttaattg aacctaagct ttcgagcacg
     3241 actttctctt catctatagc acccgccagc gcggcgaact taatcatcgc gtactcaccg
     3301 ctcacctgat acgcgccaat cggcaattca gtacgttcac gcagctcacg cacgatgtcg
     3361 aggtacgctc cagcaggttt aaccatcagg cagtctgcgc cctgggcttc atccagcagt
     3421 gactcacgaa tcgcctcgcg acggttcatt gggttcatct gatagctttt gcggtcgcct
     3481 tttaatgcgc ttcctgcagc ttcacggaac gggccataaa aggaagaggc gaacttggtc
     3541 gaatacgaca taatcgccgt atctttaaag cctgcagcgt ccagcgcctg acgaatcgcc
     3601 tgtacctggc cgtccatcgc ggcggaaggg gcgatgaagt ctgcgcctgc agcagctgca
     3661 accacggctt gcttgcctaa attttccaga gtcgcgtcgt tgtcgacgcc atgctcgcac
     3721 agcacaccgc agtgaccgtg agaagtgtat tcacagaaac aagtgtctga catgacgatc
     3781 atttctggca cagtctgctt gcagatgcgc gacatacgcg ccaccagtcc atcttcccgc
     3841 caggcgtcgc tgccggtttc atcggtatgg tgagagatgc caaaagtcat tacggaacgg
     3901 ataccggcgt tggcgatgcg ttcaatttcg cgtgccagat gtttctctgg aatgcgcatc
     3961 acgcctggca tggcttcaac ggctttgtag tcgtcaattt cttcttcaac aaagatcggc
     4021 aacaccaggt cgttaaggct aagtgttgtc tcttcaaaca tagcgcgcag cgcaggagat
     4081 ttgcgcaggc gacgagggcg ttggattaag tctgtcatgg tctgcctgat gtttgtggaa
     4141 ttaaggtgca cagtatacct gaagcggggc aggagtgttt tacgaaagtt gtcgcgatgt
     4201 tgacaagaag agaatgaaag agagaccagg acatctttgt aatagaaata gcaataattt
     4261 catattcact gaaaatattt ttaatcttta tttatagctc ttggttatta ttttttggcg
     4321 tttggttgtg ctgctataaa ttgaataatt caaactttgt tgtatattga taagatattt
     4381 atctggtttg tatttttgat atttataacg cgtatgcatc aaaaataaca ttatctgcaa
     4441 catactgaaa t
//
LOCUS       DAJTUP010000193         4384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTUP010000193 DAJTUP010000000
VERSION     DAJTUP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.196"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..315)
                     /gene="kptA"
                     /locus_tag="OO468_003920"
     CDS             complement(<1..315)
                     /gene="kptA"
                     /locus_tag="OO468_003920"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR6260834.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYH"
     gene            565..1248
                     /locus_tag="OO468_003921"
     CDS             565..1248
                     /locus_tag="OO468_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR6260835.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1245..1706
                     /locus_tag="OO468_003922"
     CDS             1245..1706
                     /locus_tag="OO468_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR6260836.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            1719..2891
                     /gene="iadA"
                     /locus_tag="OO468_003923"
     CDS             1719..2891
                     /gene="iadA"
                     /locus_tag="OO468_003923"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR6260837.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            2956..3867
                     /gene="hypT"
                     /locus_tag="OO468_003924"
                     /gene_synonym="qseD"
     CDS             2956..3867
                     /gene="hypT"
                     /locus_tag="OO468_003924"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR6260838.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(3860..4252)
                     /gene="iraD"
                     /locus_tag="OO468_003925"
     CDS             complement(3860..4252)
                     /gene="iraD"
                     /locus_tag="OO468_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR6260839.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4249..4332)
                     /gene="idlP"
                     /locus_tag="OO468_003926"
     CDS             complement(4249..4332)
                     /gene="idlP"
                     /locus_tag="OO468_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR6260840.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1014 a         1152 c         1128 g         1090 t
ORIGIN      
        1 gtggtagaga aactgtggcg gtgttttttc ggcaaaggaa atagcgacct gcgaagtcga
       61 atgcccctga accgcgcgga tgcacctacc atcactggaa taactaaaac gctttttatc
      121 gctggttgcg accacggtat ccagcaatgc acgggttaat cgcttgcccg ctttttgggc
      181 acaaagtatg agcttattga tatccgccca tccttcacgg tccagtacga taccaatcgc
      241 ctccggtttg tgtcggagga caaaacttaa aaacttgctc gtatccgcca gctctttttc
      301 gttgtacttt gccatcgcgt tttcgtccct ggattttctg tttttattta ctaatgtcat
      361 tgtcactggg tttatgaatt ttctgcaact caatcctgcg cagaaccttt tccacacagc
      421 gtaatttttc ccctatgcaa aaaacgcata gccagaaaca tacttgcatt ttccccatac
      481 ccacatggct gataaggttt ttcagttcca ggaattttcc tgactcaagc aacacacaaa
      541 cacaaaaaaa caatggggat tgttatgaca caacaagggg atgctgtagc cggggagctg
      601 gcgacagaaa aagtgggcat taaagggtat ctggcttttt ttctcaccat aatctttttt
      661 cccggtgttt tttccggaac agacagctgg tggcgcgttt ttgatttttc cgttctgaac
      721 ggatcgtttg ggcaactgcc tggtgcgaat ggagccacca cctcatttcg cggggtgggt
      781 ggcgcggggg ctaaagatgg ctttctcttt gcgctggagc tggcaccgtc agtcattctc
      841 tctctgggga ttatttcaat taccgatggt cttggcggat tacgcgccgc gcagcagtta
      901 atgacacctg tgctaaaacc gttgttgggc attccaggga tctgctcgct ggcactgatc
      961 gctaacctgc aaaacacgga tgccgccgcc ggtatgacca aagagctggc gcaggaaggt
     1021 gaaattaccg aacgcgataa agtgattttc gccgcatatc aaaccagcgg tagcgccatc
     1081 atcaccaact acttctcttc cggcgtcgcg gtgtttgcct ttctgggcac gtccgtaatc
     1141 gtacctttag ccgtcatcct ggtgtttaaa tttgtcggag ccaacatttt gcgcgtctgg
     1201 cttaacttcg aagaacgtcg caacccaacg caaggagcgc aagcatgaca actcaggtcc
     1261 gcaaaaacgt catggatatg tttatcgatg gcgcgcgccg tggctttact atcgccacga
     1321 ccaacctgct gccaaacgtg gtgatggcgt ttgtcatcat tcaggcgctg aaaattaccg
     1381 gcctgctcga ctgggtgggg catatctgtg aacccgtaat ggcgctgtgg ggattacccg
     1441 gtgaagcggc aaccgtcctt ttagccgcat taatgagtat gggcggcgca gtcggtgttg
     1501 ccgccagtct ggcaaccgct gacgcattaa ccggccacga tgtcaccgtt ttactgcccg
     1561 caatgtattt aatgggcaac ccggtgcaaa atgtcggtcg ctgcctgggg acagccgaag
     1621 tgaatgccaa atattacccg catatcatta cggtctgcgt gattaacgca ttgctgtcga
     1681 tctgggttat gcagcttatt gtttaacaag gagttaccat gattgattat accgcagccg
     1741 gttttaccct gctgcaggga gcgcatttgt atgcgccgga agatcgggga atttgcgatg
     1801 tcctcgtcgc taacggcaaa attatcgccg ttgccagcaa tatcccttct gacattgtac
     1861 cgaactgcac ggttgtcgat ctcagcgggc agatcctctg cccaggtttt attgatcaac
     1921 acgtccattt gattggcggt ggcggcgaag caggtcccac gacgcgcacg ccggaagtgg
     1981 cgctaagtcg cctgacggaa gcgggcgtca cgtcagtggt tggtctgctg ggcaccgact
     2041 ctatctctcg ccacccggaa tccctgctcg ccaagacccg tgcgctcaat gaagaaggca
     2101 tcagcgcctg gatgctgacc ggcgcttatc atgtcccttc ccgcactatt acgggttccg
     2161 tggaaaaaga cgtggcgatt atcgatcgtg tgattggcgt gaaatgcgcc atctctgatc
     2221 accgttctgc cgcaccggac gtttatcacc tggctaatat ggcggcagaa tcccgcgttg
     2281 gcggtttgct cggcggtaaa cctggcgtca ccgtgttcca catgggcgac agtaaaaagg
     2341 cgttaaagcc tatttatgac ctgctggaaa actgcgatgt gccgatcagc aagctgctgc
     2401 cgacccacgt taaccgcaac gtaccgttgt ttgagcaggc gctggagttc gcgcgcaaag
     2461 gcggcaccat cgatatcacc agcagcattg acgaaccggt cgcccctgcc gaaggtattg
     2521 cccgcgccgt tcaggcgggt attccgctgg cacgcgtcac cctcagctcc gacggcaacg
     2581 gtagccagcc gttcttcgat gacgaaggga atttaaccca tatcggtgtt gcaggttttg
     2641 aaacgttgct ggaaaccgtg caggtgctgg tcaaagacta tgatttcagt atcagcgatg
     2701 ccctgcgccc gctcaccagt agcgtagccg gtttccttaa catgagcggg aaaggcgaaa
     2761 ttctgccagg caatgacgca gacttactgg tcatgacgcc agaattgcgc attgagcagg
     2821 tatacgctcg cggcaaactg atggtcaaag acggcaaagc ctgcgtgaaa ggaacgtttg
     2881 aaacggctta aacggttgtc agccgcccta atggacgtag aatgccccca agggcggctg
     2941 acagagtaaa acgtaatgga tgactgtggt gcggttttgc ataatattga aaccaaatgg
     3001 ctttatgatt ttctgactct ggaaaaatgc cgcaattttt cccaggcggc agtcagtcgc
     3061 aacgtctcgc aaccggcatt cagccgccgc atccgtgcac tggaacaggc gattggcgtt
     3121 gaattgttta accgccaggt gacgccactg caactctcag aacaagggaa aatcttccat
     3181 tcgcagatcc gccatctgtt gcaacagtta gaaagcaacc ttgcagagct gcgtggcggc
     3241 agcgattacg cgcaacgtaa aatcaagata gccgctgcac actctctttc cctcggtctg
     3301 ttaccgtcca ttatcagcca gatgccgccg ctctttacct gggcaattga agccattgat
     3361 gtcgatgaag cggtcgataa actgcgtgaa gggcaaagtg actgtatttt ttcctttcac
     3421 gacgaagatt tgctggaagc gccgtttgac cacattcgct tatttgaatc tcaattgttc
     3481 cctgtctgcg ccagtgacga acacggagaa gcacttttta acctcgcgca gccacacttt
     3541 ccgttactga attacagccg caactcctac atggtgcgat tgattaatcg caccctgacg
     3601 cgccacagtg agttaagttt cagcaccttt tttgtctctt cgatgagcga gcttttaaag
     3661 caggttgccc tcgacggctg tgggattgcc tggctgccgg agtatgccat ccaacaagaa
     3721 attcgcagcg ggcagctcgt tgtgctgaat cgggacgaac aggtgatccc gattcaggcc
     3781 tacgcatacc ggatgaatac ccgcatgaat ccagttgccg aacgcttctg gcgtgaactg
     3841 cgcgagctgg agattgtgct tagctgacat tctcaagcgt cgcactgcgc caaccgccat
     3901 tatcccagac cagatcaaac gttagcggtt catcgtcgtg gtacagcatt atctcaaggc
     3961 tgatcgccag agtgtggcag tacgcttctt tcaaattaac ctgcaacgcc gcaatccgtg
     4021 gctcatgaaa taacagtgca gaacgcagct gacggcaata ccagctctgg agatgtttgt
     4081 cgctcgggtt atgcacttgc cactcgggca tgcctgaaac aagtaatgac ggtaatgccg
     4141 aatgaggaga attaaaaaga gtgaggagat cgctgcatac cgatttacgc agcaatgacg
     4201 ttttattgcc tgaaatttca ggtaaaacag cctgaagtga ttggcgcatc attttgcgca
     4261 ctcctgacgt ttagcaacat atgcggcttg ccctgaacac cgggcaccac tgtattgatg
     4321 ctcattttcc atagccattc ccttttgttc attacatcca tgtcagtctg agacaggcct
     4381 gaat
//
LOCUS       DAJTUP010000194         4347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTUP010000194 DAJTUP010000000
VERSION     DAJTUP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.197"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            124..1212
                     /locus_tag="OO468_003927"
     CDS             124..1212
                     /locus_tag="OO468_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR6260841.1"
                     /translation="MSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLI
                     FVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSF
                     EATFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRL
                     KMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQ
                     VRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKY
                     LTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRR
                     GDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1212..1946
                     /locus_tag="OO468_003928"
     CDS             1212..1946
                     /locus_tag="OO468_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6260842.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2413..3174
                     /locus_tag="OO468_003929"
     CDS             2413..3174
                     /locus_tag="OO468_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR6260843.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3218..4297
                     /locus_tag="OO468_003930"
     CDS             3218..4297
                     /locus_tag="OO468_003930"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6260844.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
BASE COUNT         1195 a          833 c         1073 g         1246 t
ORIGIN      
        1 attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg cgtagcgttg
       61 tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt gtcgttatac
      121 cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg tttattttgc
      181 gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc ccttagcggt
      241 ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat ctcaaactat
      301 gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga ccagcgtata
      361 cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg ttgggggtta
      421 ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac aaacatagag
      481 agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga aggtggcatc
      541 gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg tatatgccgc
      601 ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat tgccttaatg
      661 gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc tgttccccat
      721 ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat gaatcaggta
      781 cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat tatgaccgcc
      841 gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag agctgaagct
      901 gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata cctgacgcag
      961 gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg gttcgttcgc
     1021 gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc aatccacaat
     1081 gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga ttatctgaca
     1141 gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg ttttactcgc
     1201 gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca aacatcaact
     1261 gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt ggcctggatc
     1321 tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg aaacttcagg
     1381 ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc gtgatatggg
     1441 taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag gtttgtcgtc
     1501 gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga atgacgaatg
     1561 atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg gatattttat
     1621 tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg tttataaaac
     1681 gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt agcacacgta
     1741 aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag tccgcaattc
     1801 gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt aatgctcaac
     1861 ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa agatgtaaca
     1921 atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct ttttcgatga
     1981 cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt tttgccacgg
     2041 gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag agagttaata
     2101 aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg ttttttaaat
     2161 tttcaggttt tggttaaata tttgcttgca aatataggtg attttttatt gtaggctttt
     2221 gaaggctatt ataactgtgc gctaaattaa tgcgttgaat aaaagaaata acaaaattaa
     2281 gaaaagccat atttacgata tgggcaacat aaatataatc taaaaaagag taaggcgttg
     2341 taggttggtt ccgcgttcat taccttgtta gtgaacgcgg atttatcagt tgtcgaaatg
     2401 gtttttataa taatgaaaaa ttttaatgta ctaaccaaag tcgtattttg cttgtcgttt
     2461 ttatcaagcg catttcccac ctatgctgcg gatgatgtca aagtgactca aaacacttca
     2521 gtttttaaac tgaagcttag tgcaacgcga ttaatctatg aacaagggtc ggatggcgca
     2581 gcgataactg tgacaaatcc gcaggattgg ccgatgcttg tgcagagtaa agtctttgaa
     2641 gaagataaag taactgcagc gccctttatt gtcactccgc cactgttcag gcttaatcca
     2701 ggccagcaaa ataaaatgtg ggttgtgcag actgcgccag ctttacaaga cagggaaaaa
     2761 ttacactggc tatgcgccac cggcattacg cctaaaaaga gtgatgtttg ggccgagaaa
     2821 gataaagaaa aaattgctga aaatgtgaac ctgaacatcc aggttaacgt acatacctgc
     2881 ataaaactta ttaccaggcc atctggtatt gaaggcaaga tggttaatgc gagtgagaag
     2941 ttatcgtgga aaaagcaggg tgataaaatt gtcgcggtca atcagtcgcc gttctacatc
     3001 aacctttctt ctttacaggt tggcggtagc aagattgaga aactagatta tattgcgccg
     3061 ttttcgcaac atgagtttgt tatgccaaaa actagcggcc aggatattaa atggtctgtg
     3121 attactgatg aaggtggtga gagtcgtttg tttatatctc atttgcaatc ataatgtatt
     3181 acatttttat tgaatatttt tatgccatgc atattccatg ttttatcgac tgtcattaat
     3241 tttgataatg gccttacttg cgggtcagtt aagtgcccag gagtggtcat ttgattcttc
     3301 acagttggaa ggaaacgtta gtgcggacac tgtggcaatg tttaatcagg gagagcaact
     3361 ccccggaaat tatcgtgtgg aaatttatct taacggtgaa aaagttgatg ttggggagtt
     3421 tccctttcat cgtccggaat cccctgaaga aaaagagctt gttccatgtt taaccgttga
     3481 tgaccttatc cactatggca ttaaaattga taaaagcagc agtgatactg acaataaaaa
     3541 gaatcagtgt tttaaatgga actctattga aggtttgaaa gtaaattatg attttgatag
     3601 ccagcgcgta cagataaccg ttccacaact gtatctgcaa gataaaaaaa gtagtttagc
     3661 accagtgtcg ttatggaatg agggtgtagc tgcattccgc atggtgtatc agacaaatat
     3721 tgatatttcc aaacaaaatg ataatcaatc taccacgcgt aattctcgtt atggcaggtt
     3781 cactccaggg tttaatcttg gtgcctggag gtttcgtagt tcagtgacat ggagcaagga
     3841 acttggccag tcagaaagat ggcaaagggg ttacatgtgg tttgagcgag gcataaatgc
     3901 gattaaaagc cgattaacgc ttggagaatc ttatacgtcg tctgaggtgt ttgacagcat
     3961 accttttcgc ggcgggatgt tagcgaccga cgatgcgatg actccgccgg aggacagcta
     4021 ctacacacct gtggtgcatg gaatagcaca aagtgaggcg caggttatta ttaaacaaaa
     4081 tgggcaaatt atttttaccc gttctgtgcc gccagggcct tttgcgctgg ataatttacc
     4141 aacgctggcc gttggcgggg agcttgatgt tacagtgaga gagagtaatg gcgaggagca
     4201 gtatttttcc gtgccatttc aaacgccggc catcgctctg catgaaggat atttcaaata
     4261 tagtgtgatg ggggggaata taaagaagaa ggtataaaaa gctcccctgt ctccgtgggc
     4321 gaaatcacgg cgatgtacgg gcttcct
//
LOCUS       DAJTUP010000195         4302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTUP010000195 DAJTUP010000000
VERSION     DAJTUP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.198"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..1301)
                     /gene="ynjE"
                     /locus_tag="OO468_003931"
                     /pseudo
     CDS             complement(1..1301)
                     /gene="ynjE"
                     /locus_tag="OO468_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(1368..2021)
                     /locus_tag="OO468_003932"
     CDS             complement(1368..2021)
                     /locus_tag="OO468_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6260845.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(2021..3492)
                     /locus_tag="OO468_003933"
                     /pseudo
     CDS             complement(2021..3492)
                     /locus_tag="OO468_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(3465..4302)
                     /locus_tag="OO468_003934"
                     /pseudo
     CDS             complement(3465..4302)
                     /locus_tag="OO468_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1023 a         1212 c         1143 g          924 t
ORIGIN      
        1 ctactgcccg ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg
       61 taccagccgc cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag
      121 gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata
      181 ttccatgctt tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta
      241 tagaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt
      301 tcaccttttg gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa
      361 cgaatgctca ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg
      421 agcatcaact gcggttgtgc cgggatcttc acgccgaaat ccggttccgc tttcactttc
      481 ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc aggtttgcca gccgccatcc
      541 agcagacgca catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg
      601 tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc
      661 atcgctttta gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg
      721 gtatcgatgt agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccctag
      781 gccgcttcaa tgactttcca gtcaccgaca ggtttcgccg taacctcttt accttgttgc
      841 aggtcatgca gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg
      901 gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt
      961 cgcgttttga cggcgtcaac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct
     1021 ttcaggttat gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtccagccag
     1081 ctagcagaga ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc
     1141 cagccgttat aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt
     1201 tggtcaagtg taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct
     1261 aaagccattg ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattgta
     1321 gttttgagtg cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg
     1381 ttgtaatttt ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg
     1441 agatcgtgcg ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac
     1501 tggcgaaaat tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac
     1561 gcttttggct gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac
     1621 aaagtggcag gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc
     1681 acggcattac gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc
     1741 ccgacgctga actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt
     1801 gcggtgggta gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac
     1861 tgtccggcca gtgcaccaat catccatgaa aacagagtgg attttccaca gccagacggc
     1921 cccattaacg tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg
     1981 ctttctggta aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga
     2041 cataacctac ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca
     2101 gttgccataa cgcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg
     2161 cctcagtggt gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg
     2221 caatactgac tgaaaatccc accgcgaagg caatcagcac agggcgcaac atgagtgggc
     2281 atttcacgta aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta
     2341 accgtgaatc aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc
     2401 acagcagatg cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca
     2461 gcgtgtactg gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca
     2521 actggcgacg ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg
     2581 ttgctaccag ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat
     2641 ccgcgaggat cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca
     2701 gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag
     2761 tgcgccgcca gctgcgccaa aacgtaggcg gcgagtaaca gcatcagcag cagactggcg
     2821 agtgcgcctt tcgtttgttg atccgcgtcg ccctgagtta accactgcca gctaattact
     2881 gccagcgtcg gtggattacc tggcccaaga ataatcgcca catccacgac cgaaagcgac
     2941 caggcaacaa tcgccagcat cgccattgcc agcggctgta gcccagcgaa tccagcacaa
     3001 tgacctgctg caacagccgt ttttcgctca atactgccac taagatccac agcagaaatg
     3061 cgctttcttt cactgcaagg gtgaggccca gaccgatcca aatcggtcca ttggcggagt
     3121 gaaatacggg aaatagtcat atagcagccc tccgtcagca aagagcagca gggcgctggt
     3181 ggcaaaagcc acatggggaa tggcgagcag ccacggcaga cgggcgcaca tacgctgcca
     3241 tttcggccca ggccatagcg ccacaatcac cagcagggca atcaacaatg ccccgaccgc
     3301 cgcgatggtt gtcgacacca gcgttgccag taatgcctgc ggtaactgtg gatcggcaaa
     3361 taacgcctgc cagcgtgtca acgacaacgc aggcgatatc agcgtgagag ctgccgggat
     3421 cagcggtgca taaatcaccg ccaccatcgc ccacagcaaa aagattaatg cgtaccgtaa
     3481 cggcgtaacc attcttgttc cagtgcattc acccatcctg cgtgcggttc agccagtacc
     3541 ggcggcagat cctgcggcat tcttgattgc aatatttcgc gctgcccgtc aggcagtttt
     3601 tgcggatcga gaacagaagg atcgccccag acaacgggat ctgctttacg cagttgcgca
     3661 tcgggtgaga gcaggaaatt ggcaactacc ttcgccgcag cactggcatt cgcgttggct
     3721 ggaatggtga cgaaatgcac gttgccaatc atcccctcgc gaaagccaaa actgtaactg
     3781 cttgccggca aatcgccgct ggcgattttt tgctgcgcat gtgcggggtt aaaggtcagc
     3841 gacaggcgca atgtgccggc tttcagcaga gcatccatcc gcgcgggcga aggcgggaaa
     3901 tcctttcctt cgcgccacaa atacggatgc agtgcatcaa gatattgcta caagggcgca
     3961 gtgacacggg cgaaagtcgc atcgtccggc gcttctttta atgcggcggg atctggcgtc
     4021 agcataatca gcaactgttc aagaaacgcc gtgccggtaa agtccggtgg gcgcggataa
     4081 gtaaccgtgc cgggattggc tttagcaaac tccagtaagg cttgcggcgt ttgtggtggc
     4141 tgtggcgtaa catcgcggcg ggcgataaac gtcagttgtg cgccgcccca gggcgattcc
     4201 gcaccttgtg tcggcactga aaaatctccc cgcaccggca gctgtgtgtc gacatagcgc
     4261 cagttgggca gagtctccgc ccagcccgtt tgtaataaat tg
//
LOCUS       DAJTUP010000196         4289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTUP010000196 DAJTUP010000000
VERSION     DAJTUP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.199"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..260)
                     /locus_tag="OO468_003935"
     CDS             complement(<1..260)
                     /locus_tag="OO468_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR6260846.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQ"
     gene            complement(265..450)
                     /locus_tag="OO468_003936"
     CDS             complement(265..450)
                     /locus_tag="OO468_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR6260847.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(447..3086)
                     /locus_tag="OO468_003937"
     CDS             complement(447..3086)
                     /locus_tag="OO468_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR6260848.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3294..4283
                     /gene="ldhA"
                     /locus_tag="OO468_003938"
     CDS             3294..4283
                     /gene="ldhA"
                     /locus_tag="OO468_003938"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR6260849.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
BASE COUNT         1070 a         1131 c         1002 g         1086 t
ORIGIN      
        1 ttttgcccgg cgagtttcgc gatatcatct acagacacat tattttgctt tgcccgttgt
       61 tggtaactcg ctttacgttc ggcattaatt tcggttacta atttctctgt ttcagcatcc
      121 gttttcagcg caaccagata accataaaat gtttcaccta cccgcccctg agttctggct
      181 tcatccagag tcaatgccat tacattgctg cttaccagcc caacaagaaa cgcacaaaga
      241 agtaatgttt tcttcatcat cgcctcagaa aagatcgcta cgagtttcaa gcagctcttc
      301 gacatctttg tctgccttga tgatgatctc atgctcaatt ttaacgttca tattgatagt
      361 gatcggttcc ttaggtgcag cgacttcaat gcgaggcgta cagccaacca gcataaaaga
      421 tgacgtcaac gcagccagta aaattttcat tgtttttcct cacactcttt tccttgcgga
      481 cagtcatttc ccggcagacg tgcgttctgc tccaaccatg cctggagatt atcgccaaag
      541 cgtaaactgc gccacagcgt aaaaatattc tcttcatgat ggtaattaag gtttaccgta
      601 ccgcttttac catcaacgcg actggtacct gtaatgttgg cctgcatggt taataaaccg
      661 agattatcta agttaatttt tgtcgatgac cggctaattt ccatatagcg caaccagtta
      721 attgctgaac ccgcagtcat attgtctttc accaccgcat ctgcggtatc tttatccagc
      781 cgcaaggtca ttagcccgct atttgccagc caaccatctt tcactatcca tttttcgttg
      841 tttagccata acggcaatgc accactaaat gccccggaca tggcgaattg tttcggattg
      901 acggcgctaa ccagttcgct ggatgagaga ttattcagcc gcaacagcgc cgggtcatgt
      961 tgcggcatac gtaattgttt catcagcacg ttaccgccta acacatcgac gctaacatca
     1021 gtcaacagca agggttcttc ttcagtccac ggataacgcc cttgcaaatc agccgtaata
     1081 tttttcgctg tcaccagatt aatcacttcg gcaattcgca acgtaacggg gccgcgagtc
     1141 cccagatgcc aggctccatc ggcaaaacgg aaaggcagga caaaatcgac accgttaacc
     1201 tgattatctg gcatccaggc actaccgcct ttcaacacgc cgtgccctcc cgcgcggaat
     1261 ccttgttcag gcgcagcaga aaatgcaacc tgagcataca gttcaccatc gcgtaagttc
     1321 atcttccagt cgggtggcac cagcggctgg aatacagtca gtgattgttt tggccaccag
     1381 gcgttaccgc gcagacgaat accgtcccag cgaccattta cccgaaccgg gccaatctca
     1441 ccagcatgta aatcgccttt aaagaggaaa taggtcggat tgcgcccatc gacgctaaat
     1501 tttaaggttg atggcggtaa cacactgccg ccagtgaaca gcgtttgccc ggcgtccagt
     1561 gacagcgcgc cgctgaagga gggatgctgt gcgtcacgta cccagacgac gggcttgtcg
     1621 agaattaatc gcggcttttc taccgtcatc gtaccgtatt gtaactggtc gaaaccggtg
     1681 gagagatcgg tcagcgtaat cgtgctgtca tgccactcac cttttcctgc gacatcccag
     1741 gtggcattca tcggtgtaaa actccctttt ccccagtagc gccactgcca gcggccagcg
     1801 tcagggagaa aatcattcgc cagcccatcc atatgcagca cgaaatcgcc cagttcattt
     1861 tcatgcgcct gcaaaatggc ctgcaaacgt ccgtcaacac cacgttgggt gacttttaca
     1921 cccgctaaag gccagcggat ttcatcgatg tccagcgaat cgatgactct tccctttgaa
     1981 cgaagtaacg cgcctggctc aaaggtcagc gttgggtcag acagacttcc acttagctgc
     2041 gcaggtaaac gggcatataa aatgaggtcc gcctgtttcg cttcaccggt cagctgcaga
     2101 ggcagttgac tgttatccat gcttaatttt cctgggccaa aattaagcac cgcgttgccc
     2161 ttacccgctt gcccctgggt cagcacattc agtcgtccgc tgaccagagc gttctcaagc
     2221 cctgcctgcc agttgtcgac tttgacaccc agtcggccac tcaaaggaaa accagcatac
     2281 agccagctcc agcgaccatc gcttacggtc aattgttgac gagtaatttg ccacggcaaa
     2341 tcgagcaacg gatcgccgtt atcccgtgcc agcacgatca attgcccgct attttcctgc
     2401 cagtccagct cggcatccac cagtgacggt tcctgcggca agtttaacgt cgcagtagca
     2461 tgcccactta caggaagtcc atccggcacg agcggcatag taaattcccc caccagttta
     2521 accggcggct gattttcaaa cgcgacgaca tccagttcgc tgactgtaag ttgttgccct
     2581 ttcagctggc cttgaaattt aactttttcg ccctgataac gcagttgctg gatatcagag
     2641 gttaatgcga gagagagttt tccctgccat tcctgccagg gagaaaaaat cagtttatcg
     2701 atattgatcc aggtgttagg cagcatggac tgccactgcg cgagggtttt tggagcagct
     2761 ggcgattgct ccgtctgcgg caatttcgcc agacaagcag aatcaagttc taccgtgccg
     2821 acgttcaata accagcggct gggatgtgaa aggctggcgt tggtgatatg cgcaagctga
     2881 caatctccca ccagataacg gagatcggga atgattaaac ctttacgcgt aatgcgtggg
     2941 ctttcatcta atgcaatacg tgtcccgagc ggtagccaga tgtccgccag cgtgggaacc
     3001 cacagcccga gcgtcatcag cagcgtcaac ggcacaagaa taatcagtaa taacagcgcg
     3061 agaacggctt tatatttacc cagcatgggt agttaatatc ctgatttagc gaaaaattaa
     3121 gcattcaata cgggtattgt ggcatgttta accgttcagt tgaaggttgc gcctacacta
     3181 agcatagttg ttgatgaatt tttcaatatc gccatggctt tcaattatat ttgaaatttt
     3241 gtaaaatatt tttagtagct taaatgtgat tcaacatcac tggagaaagt cttatgaaac
     3301 tcgccgttta tagcacaaaa cagtacgaca agaagtacct gcaacaggtg aacgagtcct
     3361 ttggctttga gctggaattt tttgactttc tgctgacgga aaaaaccgct aaaactgcca
     3421 atggctgcga agcggtatgt attttcgtaa acgatgacgg cagccacccg gtgctggaag
     3481 agctgaaaaa gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc aataacgtcg
     3541 accttgatgc ggcaaaagaa ctggggctga aagtagtccg tgttccagcc tatgatccag
     3601 aggccgttgc tgaacacgcc atcggtatga tgatgacgct gaaccgccgt attcaccgcg
     3661 cgtatcagcg tacccgtgac gctaacttct ctctggaagg tctgaccggc tttactatgt
     3721 atggcaaaac ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc
     3781 tgaaaggttt tggtatgcgt ctgctggcgt tcgatccgta tccaagtgca gcggcgctgg
     3841 aactcggtgt ggagtatgtc gatctgccaa ccctgttctc tgaatcagac gttatctctc
     3901 tgcactgccc gctgacaccg gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga
     3961 tgaaaaatgg cgtgatgatc gtcaatacca gtcgcggtgc attgattgat tctcaggcgg
     4021 caattgaagc gctgaaaaat cagaaaattg gttcgttggg tatggacgtc tatgagaacg
     4081 aacgcgatct gttctttgaa gataaatcca acgacgtgat ccaggatgac gtattccgtc
     4141 gcctgtctgc ctgccacaac gtgctgttta ccgggcacca ggcattcctg acagcagaag
     4201 ctctgaccag tatttctcag actacgctgc aaaacttaag caatctggaa aaaggtgaaa
     4261 cctgcccgaa cgaactggtt taatcttgc
//
LOCUS       DAJTUP010000197         4215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTUP010000197 DAJTUP010000000
VERSION     DAJTUP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.200"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..532
                     /locus_tag="OO468_003939"
     CDS             <1..532
                     /locus_tag="OO468_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6260850.1"
                     /translation="VRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNAMRKAVVGPVV
                     IDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVTQAQAEADSKL
                     AAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVALTTAERWDGKL
                     PDTMIPGSTVPFISTK"
     gene            complement(578..2569)
                     /gene="tkt"
                     /locus_tag="OO468_003940"
     CDS             complement(578..2569)
                     /gene="tkt"
                     /locus_tag="OO468_003940"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR6260851.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFEAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            2847..3605
                     /gene="loiP"
                     /locus_tag="OO468_003941"
                     /gene_synonym="yggG"
     CDS             2847..3605
                     /gene="loiP"
                     /locus_tag="OO468_003941"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR6260852.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(3811..>4215)
                     /locus_tag="OO468_003942"
     CDS             complement(3811..>4215)
                     /locus_tag="OO468_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HCR6260853.1"
                     /translation="EGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQV
                     KQIVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVE
                     VAPAYDQSEITALAAATLALEMLYIQAAKKGE"
BASE COUNT          993 a         1135 c         1147 g          940 t
ORIGIN      
        1 tgtccgtcag ttgccaactc agctggagaa cattttcggc caatataccg ccatcagcgc
       61 cgtacaggat cgtacaaaat tggttcagga tctgcaaaat gccatgcgca aagccgttgt
      121 tgggccagtg gtaattgacg gggtccagat tgaaaatatt gatttttctg acgcttatga
      181 aaagtcgatt gaaaaccgaa tgaaagcaga agtcgccatt gccacgcgta agcaaaatct
      241 ggaaacagag aaaatccagg cgcaaatagc ggtaactcag gcccaggcag aagcagacag
      301 taaactggcg gcagccaaag cagaagcgga aactattcgc gtaagaggcg cggcagaggc
      361 tgaaactatt cgtttaaaaa gtgcggctga agcagaagca atccgcctgc gcggtgaagc
      421 tctgcgggat aatcccggac ttgtagccct caccactgcc gaacgttggg acggtaaact
      481 gcccgatacc atgatccccg gcagcacagt tccgtttatc tctactaagt aaaaaaaggg
      541 ccgctttcgc ggtccttctc acaaaacgtc ttactgatta cagcagcgct tttgctttcg
      601 caacaacgtt atcaacagtg aagccgaact cttcaaacag cagctctgcc ggagcagatt
      661 caccgaaggt ggtcatgccg acgatagcgc cgttcaggcc aacatacttg taccagtagt
      721 cagcaatacc cgcttctacc gcaacgcgtg cagtaaccgc tttcggcagt acggattcac
      781 ggtaagccgc atcctgcttg tcaaatgcgt cggtagacgg catggacacc acgcgcgctt
      841 tcacgccttc ggcagtcagt ttttcgtagg cagcaaccgc cagttcaact tcagaaccgg
      901 tggcgatgaa gatcagttcc ggctgaccag cacagtcttt cagcacataa ccaccgcgcg
      961 cgatgtttgc cagctgctct tcagtacgtt cctgctgcgc caggttctga cgggagagga
     1021 taagcgcagt cgggccgtcc tgacgctcaa cgccgtattt ccacgcgacc gcggattcaa
     1081 cctggtcaca cggacgccat gtagacatgt tcggggtcac gcgcagagaa gcgacctgct
     1141 ctaccggctg gtgagtcggg ccatcttcgc ccagaccgat ggagtcgtgg gtgtaaacca
     1201 tcacctggcg ctgtttcatc agcgcagcca tacgtacggc gttacgtgcg tattccacga
     1261 acatcaggaa ggtggaggtg tacggcagga agccaccgtg cagggagata ccgttagcaa
     1321 tcgcggtcat accgaactcg cgaacaccgt agtggatgta gttacctgca gcatcttcgt
     1381 tgattgcttt agaaccagac cacagggtca ggttagacgg tgccaggtca gcagagccgc
     1441 cgaggaattc aggcaacagc gggccgaacg cttcgatagc attctgcgac gctttacggc
     1501 tggcgatttt cgccggatta gcctgcagtt tagcgataaa ctctttcgct ttggcgtcga
     1561 agtcagacgg catttcgcct ttcatacggc gggtaaattc agccgcttcc tgcggataag
     1621 ctttcgcgta agcctcaaac ttctcattcc atgcagattc tttcgcctgg cctgcttctt
     1681 tcgcatccca ctgagcatag atttcagacg ggatttcgaa cggtgcatat ttccagccca
     1741 gctgttcgcg ggtcagggca atttcagcgt cgcccagcgg cgcaccgtgg gagtcgtgag
     1801 taccggcttt gttcggggaa ccgaaaccga tgatggtttt gcacatcagc agggacggtt
     1861 tgtcagtcac tgcgcgcgct tcttcaactg cgcgtttgat ggatgccgcg tcatgaccgt
     1921 cgatgtcgcg aataacgtgc cagccgtaag cttcgaaacg cattgctgtg tcgtcggtga
     1981 accagccttc aacgtgacca tcgatagaaa taccgttgtc atcgtagaac gcaatcagtt
     2041 tacccagctt cagcgtaccc gccagagagc aaacttcgtg ggagatgcct tccatcatgc
     2101 agccgtcgcc catgaaggcg taggtgtagt ggtcgacaat gtcgtggccc ggacggttaa
     2161 actgcgccgc cagcgttttt tctgcaatcg ccataccgac tgcgttggca ataccctgac
     2221 ccagcggacc ggtggtggtt tccacaccag cggtataacc tacttccgga tggcccggag
     2281 ttttagagtg cagctgacgg aagtttttca gttcttccat cggcagatcg taaccggtga
     2341 ggtgcagcag gctgtagatc agcatggagc cgtggccgtt ggacagcacg aagcggtcac
     2401 ggtcagccca ggacggattc tgcgggttgt gtttcaggaa atcacgccac aggacttcgg
     2461 caatgtcagc catacccata ggggcacccg ggtgaccgga tttggctttc tgtactgcgt
     2521 ccatgctcag cgcacgaata gcattggcaa gctctttacg tgaggacatt ttgactccag
     2581 atcggatgat gaagggcacg cccttaacga cttgacgaca gcgcgttttg ggctacgccg
     2641 gaaaatttgc caacaattta ccgcaagccg cgcgtcatgt acatggaaca tccttttgcc
     2701 gcttcagaaa tctctggatc atgctcgcat gttgcgcaat ctgctcgccc gtccgctgcg
     2761 cttttcctta tactgagact gagcgtcgat tcacctgcaa acggcgcatt tttagaataa
     2821 tcctgacctt gtgcggaaga gaaaacatga aaattcgcgc cttattggta gcaatgagcg
     2881 tggcaacggt actgaccggt tgccagaata tggactccaa cggactgctc tcatcaggag
     2941 cggaagcttt tcaggcttac agtttgagtg atgcgcaggt gaaaaccctg agcgatcagg
     3001 catgtcagga gatggacagc aaggcgacga ttgcgccagc caatagcgaa tacgctaaac
     3061 gtctgacaac tattgccaat gcgctaggca acaatatcaa cggtcagccg gtgaattaca
     3121 aagtgtatat ggcgaaggat gtgaacgcct ttgcaatggc taacggctgt atccgcgtct
     3181 atagcgggct gatggatatg atgacagata acgaagtcga agcggtgatc ggtcacgaga
     3241 tggggcacgt ggcgttaggc catgtgaaga aagggatgca ggtggcgctt ggtacaaatg
     3301 ccgtgcgagt agctgcggcc tctgcgggcg ggattgtcgg cagtttatct caatcacaac
     3361 ttggtgatct gggcgagaaa ttagtcaatt cgcaattctc ccagcgccag gaagcagaag
     3421 ccgatgatta ttcttacgat cttctgcacc aacgcggcat cagcccggca ggtcttgcca
     3481 ccagctttga aaaactggcg aagctggaag aaggtcgtca aagctcaatg tttgacgacc
     3541 atcctgcatc cgccgaacgt gcccagcata ttcgcgatcg catcagcgcg gatgggatta
     3601 agtaaagcct agtggtgtcg aacgtaggtc agataaggcg ttcacgccgc atctgacatt
     3661 aatggcacat ttgttacctt gtgcgcattg ccggatgcga tgctggcgca tcttatccgg
     3721 cctacgggtg ccgaacgtag gtcggataag gcgttcacgc cgcatccgac attaatggca
     3781 cgttttaccc gtgcgcatcg catctggtgc ttactcgccc tttttcgccg cctgaatata
     3841 cagcatctcc agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc
     3901 cggagccact tccactacgt ccatcccaac gatgttgaga tctttcaggc cgcgtaccag
     3961 tttaatggcg cgatcggagg tcaggccgcc aatcactggc gtaccggttc ccggcgcgaa
     4021 agcaggatcc aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg
     4081 tttcacctgg gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac
     4141 ggtaaagccg ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg
     4201 gtcgatcaga ccttc
//
LOCUS       DAJTUP010000198         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTUP010000198 DAJTUP010000000
VERSION     DAJTUP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.201"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO468_003943"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO468_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6260854.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO468_003944"
     tRNA            complement(324..399)
                     /locus_tag="OO468_003944"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO468_003945"
     tRNA            complement(406..480)
                     /locus_tag="OO468_003945"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO468_003946"
     tRNA            complement(597..681)
                     /locus_tag="OO468_003946"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO468_003947"
     tRNA            complement(690..765)
                     /locus_tag="OO468_003947"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO468_003948"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO468_003948"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR6260855.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO468_003949"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO468_003949"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR6260856.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO468_003950"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO468_003950"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR6260857.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTUP010000199         4052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTUP010000199 DAJTUP010000000
VERSION     DAJTUP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.202"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(896..1087)
                     /locus_tag="OO468_003951"
     CDS             complement(896..1087)
                     /locus_tag="OO468_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR6260858.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1152..1475
                     /locus_tag="OO468_003952"
     CDS             1152..1475
                     /locus_tag="OO468_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR6260859.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1417..2175
                     /locus_tag="OO468_003953"
                     /pseudo
     CDS             1417..2175
                     /locus_tag="OO468_003953"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2153..2683
                     /locus_tag="OO468_003954"
     CDS             2153..2683
                     /locus_tag="OO468_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR6260860.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2690..2923
                     /locus_tag="OO468_003955"
     CDS             2690..2923
                     /locus_tag="OO468_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR6260861.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3469..3861)
                     /locus_tag="OO468_003956"
     CDS             complement(3469..3861)
                     /locus_tag="OO468_003956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260862.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          969 a          922 c         1208 g          953 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctaacac ccgctgacaa gcccgtcaga
      901 ggttttcacc gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt
      961 cgtaaagcga ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca
     1021 gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1081 tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga
     1141 aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac
     1201 gctgtgaggg taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg
     1261 cccgttccgg gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg
     1321 ggaataacct gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc
     1381 gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg
     1441 ccgtcaggga acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt
     1501 ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg
     1561 cttcagggta atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa
     1621 tacacgtccg gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa
     1681 gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt
     1741 ctgtgggtgg agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg
     1801 gagctacaga ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt
     1861 gatgcctggc agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag
     1921 aaccgccgga cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag
     1981 agcattacac ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat
     2041 gtgattcagg cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc
     2101 agcattgcag acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa
     2161 tcttttacag acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga
     2221 gaaaatgctg agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc
     2281 aaacgagacg aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact
     2341 gaaaaaacat cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa
     2401 gctggcaggg aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag
     2461 tggcgggacg ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa
     2521 gcagaacgag agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg
     2581 gagcaacgga cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat
     2641 ggaaaacggg aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct
     2701 ggaaagccat ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa
     2761 cgaatgggag agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa
     2821 cgcgatgctg aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca
     2881 gacagaacgc ttgagccagt tatacagcga gaactggaga taagagagga acggttgata
     2941 caggagcgcg aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg
     3001 acactggatg gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg
     3061 tttccggtgc attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc
     3121 agaccttgct gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc
     3181 ttttgtcgct gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt
     3241 tcccgctcag gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg
     3301 tctctggctg gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa
     3361 tttaccgggt gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc
     3421 tgccgtgatc gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt
     3481 gtgagggaat ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga
     3541 ttgggtttcg gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa
     3601 ggcgtgagat tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct
     3661 tccgtccgat ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac
     3721 ttcttgaatt tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt
     3781 aggggagtgt attattctct cttttaataa gctgtttacc acagggttta gttgttgtct
     3841 aaagcacgca gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg
     3901 ggtatgccgt gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc
     3961 aacaaatacg gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct
     4021 tttttgcggc gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTUP010000200         4049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTUP010000200 DAJTUP010000000
VERSION     DAJTUP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.203"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(61..918)
                     /gene="sitD"
                     /locus_tag="OO468_003957"
     CDS             complement(61..918)
                     /gene="sitD"
                     /locus_tag="OO468_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR6260863.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(915..1772)
                     /gene="sitC"
                     /locus_tag="OO468_003958"
     CDS             complement(915..1772)
                     /gene="sitC"
                     /locus_tag="OO468_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR6260864.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1769..2596)
                     /gene="sitB"
                     /locus_tag="OO468_003959"
     CDS             complement(1769..2596)
                     /gene="sitB"
                     /locus_tag="OO468_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR6260865.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2596..3510)
                     /gene="sitA"
                     /locus_tag="OO468_003960"
     CDS             complement(2596..3510)
                     /gene="sitA"
                     /locus_tag="OO468_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR6260866.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1094 a          999 c          960 g          996 t
ORIGIN      
        1 ccttccatgg ctttccggac ttctgtaggc tggcggacga tgctgtcctc ggatgttgtg
       61 ctaattgggt gataacagat cctgtgtatg ggcgtttccc tttgtgcgtg cgttgatgct
      121 ggtaacggta aacgtcatga tgaacacgac cgcgaataag acgacaatcg tcggagctgg
      181 tgcgctgtcg ataaaaaagg acagccagac accagtcatt gacactatta ccgatatgcc
      241 agtcgccagt aggagcgcaa tatgaaagcg ttgtgtcagg agtactgcaa tggcgccagg
      301 cgcaatgagc agagaaatag agagaatgat acctactgct ttcagcgtcg ccacaatggt
      361 cagggaaacc atgcacagca gcccataatg cagccatctc gtatgcaaac cgcttacctg
      421 cgcctgttga taatcaaagc tgaacagcaa aaaatcccgc cattttacgc taataacaag
      481 tgtaaccagc ccggcaataa tcaccgtctg gattatgtcg ccgatggtta tcccgagcat
      541 gtcgccgaaa agaatatggt caagatgtac gtctggttta attgcaatat acaagatcag
      601 acctgcggca aacatgccag aaaaaacaat ccccataact gtatcttgct tgattcggct
      661 gttgtctttc aggtatcccg tcgccaccgc gcagaatact ccggcgacga atgcccctgt
      721 agccagaggt aaccccagaa tccaggtaag cacgatgcca ggaaaaaccg catgactcat
      781 cgcgtcaccc atcagtgccc aacctttcag gaccagaaag accgacagca gcgcacaggg
      841 tatggaaact accagtgaaa tcagcagtgc gtgactcata aaggtgaact gcagtggctc
      901 cagtaataat gccatcatga gcgatcctcc agcgctttat gcgcacgccg acgatttgcc
      961 aaaagtccat gggtgggcgc aaatacaaat gctaaaagaa acagcagtgt ctgcgccacc
     1021 acgatgattc cacctgtcgc gccgtccaga aagtaactga cccacgcgcc gaggaaactg
     1081 gttacactgc caatagttac agcaattatg agcaggcggg gaaaacgatc ggttaacaac
     1141 catgctgttg caccgggagt caccaccaga cagatcacca gaaaggcacc aaccgtttgc
     1201 agcgccgcaa cggtcgaaac cgacagcagg gtgaagaaga ggatttttaa tctgcccgga
     1261 tgtaatccaa tagcacgggc gtgattctcg tcgaaaaagg tcaccataag atctttccat
     1321 ttgaaaaaaa gcacgatgat cgagagaatg ccaatgatag tgagttgcag gatatccgcc
     1381 ggatcgattg ccagaatatt gcccaggacg atggtctgaa tgttcacgga tgtcgggttc
     1441 aatgacacca taaacagccc caggccaaaa aaggacgaga agatcaggcc gatgatggca
     1501 tcttctttta agcgggtgcg ttggttaaga aacagcatgc tgcccgctgc cagtccgccg
     1561 gaaaagaagg ctcccagaga aaacggaagc cccagcatat atgcccccgc aacacccggg
     1621 acaatagagt gcgacagcgc atcgccaatc agcgaccagc cttttagcat cagatagcat
     1681 gacaggaaag cgcagagacc gccgaccatt gccgagaccc acatcgcatt gagcatatat
     1741 tcatagctga agggttccag tagcacgttc atcttgcttc cctctgcacc gctgacggtc
     1801 gatgtgccac aaaagggcgt tcatcgtcag taataatgct ttcttccgag ccgttaagtg
     1861 tgacatggcg cagtacgccg ctaaaagcca gctccaggtt ctcggctgta aaagtggtgt
     1921 ctgtcggccc gcttgccagg acggtgcctt tgaccatgac cgtataatcg caaaatgtcg
     1981 tgactgagcc gagattgtgc gttgagacaa gcatcgtttt gccttccgcg cgtaactccc
     2041 tcaacaggct gatgattttc gcttcggttt tcacatcaac gccggtaaaa ggttcatcca
     2101 gcaggatcac atcaccctgc tgtgcaattg ctctcgccag aaagacgcgt ttcttttgtc
     2161 caccggaaag ctcgccgatt tgacgatggc gaaaatccac catatcgaca cgttccagcg
     2221 catcggtgac aatctgacga tccctctttt tggctatacg caacataccc atatgcccat
     2281 agcggcccat catcaccaca tcttccacca gtacaggaaa tgaccagtca acctcttctg
     2341 actgagggac gtaggcaacc aggtttttct gtagcgcctg tcgcgtggga atacccagaa
     2401 cagatatctt tccgctggtc agacgcacaa atcccataat cgctttaaac aacgtcgatt
     2461 ttccggaacc gtttacccca accagagcgg caatagatcc acttggtact gtgaaggatg
     2521 catcgcgcag tgctgtgtgc ccgttacgcc aggtgacagt aacatcatta acgacaatgc
     2581 ctgcagattg catcattatt tctccctttt cccggcttta attccctgta ccagcgtact
     2641 ggtagtaacc ttaagaaggt cgatatacgt aggtaccggg ccgttttctg tgctcaggga
     2701 atcgacatag agcacaccgc cgtagtgtgc gccggtttca cgcgcaacct gacgcgctgg
     2761 tttatcggaa atcgtactct cgctaaagac tgccgggata tgatttttct taactatatc
     2821 aacaacctta cgtacctgct gcggtgttcc ttgttgatcg gcattaatcg gccacagata
     2881 aagctctttt agccccaaat cgcgtgcgag ataagaaaaa gccccttcac tggtgaccat
     2941 ccatcgctga ttctcaggga gttccgtaat ctgcttacgc aggggggcaa gggtttgggt
     3001 aatcttggct ttataagtat cggcattacg ttggtaggtt tgtgcatttg ccggatcgta
     3061 ttttatcaac gcatcacgaa tattatcgac gtaaatcaga gcattatctg gcgacatcca
     3121 tgcatgggga ttaggtttgc cctcataggg tccttcggtg atccctactg gcgtcacacc
     3181 cgaagagaca attacttctg gaaccccatt gagatgctgg taaaagcgtt ggaaccacaa
     3241 ttccagattc ataccattgg cgagaatcag ttgtgccccc tgcgcacgtt taatatcgcc
     3301 aggggtaggc tgatactcat gaatttctgc accaggcttg gttatggatg agacttctgc
     3361 agcatctcca gccacgtttt tggccatatc cgcgatgatg gtaaatgttg taatgacctt
     3421 gaatttttca gttgcacttg cctgaaatgc gatcgagcag gtgagggcga ggcaccccaa
     3481 gagcatggtt acttttttta ttgagtgcat aatttagtac ctatagtgag caaaattaga
     3541 acaaacatag caacggctat gttttatagc ataacctata caaaatgaaa ataattatca
     3601 ttaacagctt tgaggcctaa aacagggatt aggttgtttc ctgctggcga catgatattg
     3661 aggaaaaaag tagagcagct attaggcttt gaaatgactt tatgaggaaa attgttgatg
     3721 gcaaaaatgg ggcaaaaaag agtgggttat cgtagcttat tgttgtcgct gataatgttt
     3781 aacatattga aaaataaata aaatacttat gagtcagata gttgtgattt ttgcccttac
     3841 ttgttcaggt tgtattgttc tttctttgta atttgttgat tttcttgcat tatttcagtt
     3901 ctctggtact aaatggggca aattgggggc aaactttgca actacgataa ccgcgcattc
     3961 aacatggcta tctgttcgtc gttcatgtca tcaatccaca taccgtaaat ttcatacacc
     4021 atttgcgcag tttcatgccc catctggct
//
LOCUS       DAJTUP010000201         3970 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTUP010000201 DAJTUP010000000
VERSION     DAJTUP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.204"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..177)
                     /locus_tag="OO468_003961"
     CDS             complement(<1..177)
                     /locus_tag="OO468_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6260867.1"
                     /translation="MSRKTRRYSKEFKAEAVRTVLENQLSISEDASRLFLPEGTLGQW
                     VTAARKGLGTPGSRT"
     gene            203..466
                     /locus_tag="OO468_003962"
                     /pseudo
     CDS             203..466
                     /locus_tag="OO468_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            512..721
                     /gene="hha"
                     /locus_tag="OO468_003963"
     CDS             512..721
                     /gene="hha"
                     /locus_tag="OO468_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR6260868.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            760..1350
                     /locus_tag="OO468_003964"
     CDS             760..1350
                     /locus_tag="OO468_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR6260869.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1590..1850
                     /gene="repA"
                     /locus_tag="OO468_003965"
     CDS             1590..1850
                     /gene="repA"
                     /locus_tag="OO468_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR6260870.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            2074..2148
                     /gene="tap"
                     /locus_tag="OO468_003966"
     CDS             2074..2148
                     /gene="tap"
                     /locus_tag="OO468_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR6260871.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            2141..2998
                     /gene="repA"
                     /locus_tag="OO468_003967"
     CDS             2141..2998
                     /gene="repA"
                     /locus_tag="OO468_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR6260872.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3699..3890
                     /locus_tag="OO468_003968"
     CDS             3699..3890
                     /locus_tag="OO468_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260873.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRVIQAAFNAFAKV"
BASE COUNT         1074 a          964 c          993 g          939 t
ORIGIN      
        1 cgtgcgggaa ccaggagtac cgagcccttt tctggcggcg gtaacccatt gtcctaaagt
       61 gccttcagga aggaataatc gggaagcgtc ttcactgatc gaaagttgat tttcaagaac
      121 cgttctgaca gcttcggctt tgaactcttt agagtaacgt cgggtttttc tgctcattat
      181 tagctccttc tgatgccatt ctatttcagg aaggagtgtc cgttaaactc aggctacctc
      241 atattttcag cgagatcgtt atggtcgtat tctcgggcag gtttatgcac cagacggaat
      301 gaatattaat cagtttatgg tccgggccgg ggctgcctgg gtatatgagc agtacaacac
      361 agatcccgtg ctacctgtcc ttcagaacga agccagacaa caaaaacgtg ggctctggtc
      421 agatgctgat ccggttcccc cctggatatg gaggcaccgt aaataaagcg atggcaatcc
      481 tatcattcat ctgttctcag cgggaataaa aatggcaaaa acaaaacagg aatggctgta
      541 tcagttacgg cgatgttcat cagtcaatac gctggaaaaa attattcata aaaacagaga
      601 cagtttatca aacagtgaac gtgaatcatt taattcagca gcagaccacc ggctagcgga
      661 attaatcacc ggaaagttat atgacaggat accaaaagag atatggaaat atgtaaggta
      721 ataattcagc aataattaat tgccaggagc agataaataa tgaagttgat tatttttatt
      781 ctcattgttc tgattattgc agcgctactt atccggatta tcctcagaag tgtaaatcag
      841 cattctcctt tgctgatgca attgcatgcg gcaggtatac ggactggtga tgcagagcga
      901 atactgtcca gcggtgaata ctggcaacgt cagaagacgc tgctgacaga aagggaagtc
      961 agttttatga aaggactgtt cagaattgtg gatatgaagc ggtggtatct gtgtccgcag
     1021 gtacgggtag cggatatcgt ccagctgaac gggaatatcc ggccacgatc acgccagtgg
     1081 tggcagttat tcaggatggt gtctcagtgg catgttgatg tggttatcgt tgagctgcgt
     1141 tcgttcagta ttgttgcagc agtagagctg gatgatgcca gccatttacg accggaacgc
     1201 agacgccggg atattcttct ggaagaggtt ctgaggcagg ctggtattcc gttgctcaga
     1261 agccacgatg ccagaaaact gctgcagatg accggagaat ggctgaatac aacaggggct
     1321 gatcagcagt caccggaaca tcgtagctga cgccttcgcg ttgctcagtt gtccaacccc
     1381 ggaaacggga aaaagcaagt tttcctcgct cccggcgttt ccataactga aaaccatact
     1441 atttcacagt ttaaatcaca ttaaacgaca gtaatccccg ttgatttgtg cgccaacaca
     1501 gatcttcgcc acaattctca agtcgctgat ttcaaaaaac tgtagtatcc tctgcgaaac
     1561 gatccctgtt tgagtattga ggaggcgaga tgtcgcagac agaaaatgca gtgacttcct
     1621 cattgagtca aaagcggttt gtgcgcagag gtaagcctat gactgactct gagaaacaaa
     1681 tggccgctgt tgcaagaaaa cgtcttacac acaaagagat aaaagttttt gtcaaaaatc
     1741 ctctgaaaga tctcatggtt gagtactgcg agagagaggg gataacacag gctcagttcg
     1801 ttgagaaaat catcaaagat gaactgcaga gactggatat actaaagtaa agactttact
     1861 ttgtggcgta gcatgctaga ttactgatcg tttaaggaat tttatggctg gccacgccat
     1921 aaggtggcag ggaactggtt ctgatgtgga tgtacaggag ccagaaaagc aaaaaccccg
     1981 ataatcttct ttaactttgg cgagtcagaa agattaccgg ggcccactta aaccgtatag
     2041 ccaacaatca agctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt
     2101 ctgtgctcgc tccttctgcg cattgtaagt gcaggatggt gtgactgatc ttcaacaaac
     2161 ttattaccgc caggtaaaga acccgaatcc ggtgttcaca ccccgtgaag gtgcccggac
     2221 gcttccgttc tgcgggaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt
     2281 cgccattcat gtggcacatg cccgttcgct tggtctgcgt cggcgcatgc caccggtact
     2341 acgtcgccgg gccattgatg cgctgctgca ggggctgtgt tttcactatg acccactggc
     2401 caaccgcgtc cagtgttcca tcaccacgct ggccattgag tgcggactgg cgacggagtc
     2461 tgctgccgga aaactctcca tcacccgggc cacccgggcc ctgacgttcc ttgcagagct
     2521 gggactgatt acctaccaga cggaatatga cccgcttatc gggtgctaca ttccgaccga
     2581 tatcacgttc acatctgcac tgtttgctgc cctcgatgta tcagaggagg cagtggccgc
     2641 cgcgcgccgc agtcgtgtgg aatgggaaaa cagacagcgc aaaaagcagg ggctggatac
     2701 cctgggtatg gatgaactga tggcgaaagc ctggcgtttt gttcgtgagc gtttccgcag
     2761 ttaccagaca gagcttaagt cccgtggaat gaagcgtgcc cgtgcgcgtc gtgatgcgga
     2821 caggcagcgt caggatattg tcaccctggt gaaacggcaa ctgacgcgtg aaatctcgga
     2881 agggcgcttc actgccagtc gtgaggcggt aaaacgtgaa gtggagcgtc gtgtgaagga
     2941 gcgcatgatt ctgtcacgta accgtaatta cagccggctg gccacagctt ccccctgaaa
     3001 gtgacctcct cagaataatc cggcacgcgc cggaggcatt cgcacgcctg aagcccgcca
     3061 gtgcacaaaa aatcagcgct acatacaaaa aacaacctca tcatccacct tcaggtgcat
     3121 ccggtccccc ctgtttttga tacaaaacac gcctcacaga cggggaattt tgcttatcca
     3181 cattaaactg caagggactt caccataagg ttacaaccgt tcacgtcata aagcgccaga
     3241 cgtcaaccct acagggtgca atgtatcttt taaacacctg tttatatctc ctttaaacta
     3301 cttaattaca ttcatttaaa aagaaaacct gttcactgcc tgtcctgtgg acagacaaat
     3361 atgcacctcc caccgcaagc ggcgggcccc caaccgaagc cggtttagtt acaacatgca
     3421 aacataaacc cctaaaaaac ccaagtccaa cgcaaaactg aaaccacaac gcccccctca
     3481 taactgaaaa gcgccgccgc ccccgcccaa agggcgggaa cagagtcgct tttaattatg
     3541 aatgttgtaa ctacatcatc atcgctgtca gtcttctggc tggacgtatc gaatacacgc
     3601 tcgtaagcgg ccctcacggc ccgctaacgc ggagatacgc cccgacttcg ggtaaaaccc
     3661 tcgtcgggac cactccgacc gcgcacagaa gctctatcat ggctgaaaac gggtatggct
     3721 tggcagggct ggggatgggt aaggtgaaat ctgtcaatca gtaccggctg acgccgggct
     3781 tcggcggttt tactccggta tcacatgtaa ccacagcgtg ccgccttcca tgccgctggc
     3841 gcggcatcag agtaatacaa gcagcattta atgcatttgc aaaagtgtga gtaatattaa
     3901 gtggtcgttt cagggatatc gttaagactt gagcgatagg aattaaaacc ccaaaaagat
     3961 taaaaaaaca
//
LOCUS       DAJTUP010000202         3878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTUP010000202 DAJTUP010000000
VERSION     DAJTUP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.205"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            34..2313
                     /gene="ydeP"
                     /locus_tag="OO468_003969"
     CDS             34..2313
                     /gene="ydeP"
                     /locus_tag="OO468_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR6260874.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2561..2764
                     /gene="safA"
                     /locus_tag="OO468_003970"
     CDS             2561..2764
                     /gene="safA"
                     /locus_tag="OO468_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR6260875.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            2832..3593
                     /gene="ydeO"
                     /locus_tag="OO468_003971"
     CDS             2832..3593
                     /gene="ydeO"
                     /locus_tag="OO468_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR6260876.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
BASE COUNT         1092 a          839 c          890 g         1057 t
ORIGIN      
        1 tcctaataat tcatttccac acaggataag tagatgaaga aaaaaattga atcctaccag
       61 ggtgctgcag gtggttgggg tgctgttaaa tccgtagcga atgcagtacg taagcagatg
      121 gatatacgcc atgatgttat tgccatgttt gacatgaata agccagaggg ctttgactgt
      181 ccgggttgtg catggccaga tcctaagaac agtgcgtcat tcgacatttg tgaaaacggc
      241 gcaaaagcaa tcgcttggga agtcacggat aagcaggtaa atgcctcttt ctttgctgag
      301 aatacggttc aatcattact tacctgggga gatcacgagc ttgaggctgc ggggcgtctc
      361 actcagcctt tgaaatatga tgccgtcagc gactgttaca agccattaag ctggcaacaa
      421 gctttcgacg aaattggcgc acgccttcaa agctatagtg atcccaatca ggttgaattc
      481 tatacttcgg gccgcacttc caatgaagct gcctttcttt atcagctttt tgcccgtgaa
      541 tacgggagca ataactttcc cgactgctcc aacatgtgcc atgaaccgac aagcgtgggt
      601 ttggcagcga gtatcggtgt aggtaaaggg accgtgttgc tggaagactt tgagaagtgc
      661 gatttagtca tttgcattgg gcataaccct ggtacaaacc accctcgcat gctgacttcg
      721 ttgcgcgctt tagtgaaacg gggagcgaaa atgatcgcca tcaatcctct acaggaacgt
      781 ggcctggagc gatttaccgc accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact
      841 cagttggcca gtgcctacta caacgtgcgc attggtggcg atatggcgtt gctcaagggg
      901 atgatgcgcc tgttaattga gcgcgatgat gctgcaagtg ccgcaggtcg gccctcattg
      961 cttgatgacg aatttattca aacgcatacc gtcggctttg acgagctacg ccgtgacgtt
     1021 ctcaattccg agtggaaaga tatcgaacgt atttctggac taagtcagac acaaatcgcc
     1081 gaactggctg acgcatatgc cgctgccgaa cgcaccatta tctgttacgg aatggggatc
     1141 actcagcacg aacatggtac ccagaacgtg cagcaactgg tcaatttgct gttgatgaaa
     1201 ggtaacattg gcaagcctgg tgcgggtatc tgcccactac gtggtcactc taatgtacag
     1261 ggcgaccgaa ccgtcggtat caccgagaaa ccgtctgcag agtttctggc tcgcctgggt
     1321 gagcgctatg gcttcccccc acctcatgca cctggacatg ctgcaattgc cagcatgcaa
     1381 gcaatatgta cggggcaggc tcgagcattg atctgcatgg ggggcaactt tgcactggca
     1441 atgccagatc gggaagcgag cgctgtaccg ttaacgcaat tagatttggc ggtacacgta
     1501 gccactaagc ttaaccgctc tcatctgctg accgcacggc atagctatat tctgccggtt
     1561 ctggggcgta gcgagattga tatgcaaaaa agcggtgcgc aggcggtaac cgttgaggat
     1621 tcaatgtcga tgattcatgc ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa
     1681 tcagagtgtg cagtggtcgc gggaatcgcg caggcagcac taccccagag cgtggtagcc
     1741 tgggagtatc tggtggaaga ttatgatcgc attcgcaatg acattgaagc tgtgctgcca
     1801 gagttcgccg actataacca gcgcatccgt catcccggtg gttttcacct gataaatgca
     1861 gctgctgaaa ggcgctggat gacgccgtca ggtaaggcta atttcattac cagcaaaggg
     1921 ctgttagaag atccctcttc agcgtttaac agtaagctgg tcatggcgac agtacgcagc
     1981 cacgatcagt acaacacgac gatttatggt atggatgatc gctatcgagg ggtattcggt
     2041 caacgagatg tggtctttat gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc
     2101 gaaagagtta atcttattgc gcttacgcca gacggtaagc gcagctcacg ccgcatggat
     2161 agattaaaag tggtcattta ccctatggct gaccgctcac tggtgaccta ttttccagaa
     2221 tcgaatcaca tgctaacact tgataaccac gatccattaa gtggtattcc tggctataaa
     2281 agtattccgg ttgaattaga accatcaaat taatgcctct tctcatttct tctgctgtca
     2341 tccgcacagc agaagaattc ctcattgact attattgcgc aatttgctca catggattaa
     2401 attaaactac atactataag atataaactt ctgcctacag ctgtaagaaa ctccgctcag
     2461 tactgaagca ccagtcctat ttcttctttt ctccagcctg ttatattaag catactgatt
     2521 aacgactttt aacgttatcc gctaaataaa catatttgaa atgcatgcga ccacagtgaa
     2581 aaacaaaatc acgcaaagag ataactataa agaaatcatg tctgtaattg tggttgtctt
     2641 attactgaca cttacgttga tagccatttt ttcggcaatt gatcagctga gtatttcaga
     2701 aatgggtcgc attgcaagag atcttacaca tttcattatc aatagtttgc aagactggaa
     2761 ataaacagca aattataaat gtgaaatgtt taaaaggtat cgataaaact ttattgtttt
     2821 aaggagataa aatgtcgctc gtttgttctg ttatatttat tcatcatgct ttcaacgcta
     2881 acattttaga taaagattac gccttctccg acggcgagat cctgatggta gataacgctg
     2941 ttcgtacaca ttttgaacct tatgagcggc attttaaaga aatcggattt aatgaaaata
     3001 ccattaaaaa atatctacaa tgcactaaca tccagacagt gacggtgcct gttcctgcga
     3061 agtttttacg tgcttcaaat gtaccgactg gattgcttaa tgaaatgatt gcttatctca
     3121 actcggaaga acgcaatcat cataattttt cagaactttt gcttttttct tgcctgtcta
     3181 tttttgccgc atgcaaaggt ttcattacac tattaactaa cggtgtgcta tccgtttccg
     3241 ggaaagtgag aaatattgtc aacatgaagc tggcgcaccc atggaagctg aaagatattt
     3301 gtgactgcct gtacatcagt gaaagcctgt tgaagaaaaa actaaagcaa gagcaaacga
     3361 cattctcaca gattctatta gatgccagaa tgcagcacgc aaaaaatttg atacgcgtag
     3421 aaggttcagt caataaaatt gccgaacaat gtggttatgc cagtacatct tattttattt
     3481 atgcgttccg caaacatttc ggcaacagtc cgaagagagt ttctaaggag taccgttgtc
     3541 aaaggcacac gggtatgaat acgggcaaca cgatgagtgc tttagctatt tgattatttg
     3601 ctaacgagta gtcaaccacg cacgctacgt aagaattata tggggcagcc attccctgcc
     3661 ccgcgttgtt tttaggcgat atatttattg aaataaataa gtgacatcca tcacatattt
     3721 atgcacttgc ataacctgtt gcatgattat ttatgatctc aattcggcat tttgtcagta
     3781 aaatgcaata atttattaaa tatcaataaa ttagttgttt atcggcgaga aattacttaa
     3841 tagaacagaa agtaatgtca acgctttatg gactgtta
//
LOCUS       DAJTUP010000203         3829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTUP010000203 DAJTUP010000000
VERSION     DAJTUP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.206"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(23..184)
                     /locus_tag="OO468_003972"
     CDS             complement(23..184)
                     /locus_tag="OO468_003972"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260877.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(181..369)
                     /locus_tag="OO468_003973"
     CDS             complement(181..369)
                     /locus_tag="OO468_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR6260878.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            629..964
                     /gene="iraM"
                     /locus_tag="OO468_003974"
     CDS             629..964
                     /gene="iraM"
                     /locus_tag="OO468_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR6260879.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1245..1353)
                     /locus_tag="OO468_003975"
                     /pseudo
     CDS             complement(1245..1353)
                     /locus_tag="OO468_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1876..1952)
                     /locus_tag="OO468_003976"
     tRNA            complement(1876..1952)
                     /locus_tag="OO468_003976"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1916..1918),aa:Arg,seq:tct)
     gene            complement(2054..2129)
                     /locus_tag="OO468_003977"
     tRNA            complement(2054..2129)
                     /locus_tag="OO468_003977"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2094..2096),aa:Ile,seq:cat)
     gene            complement(2274..3095)
                     /locus_tag="OO468_003978"
     CDS             complement(2274..3095)
                     /locus_tag="OO468_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6260880.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3110..3466)
                     /locus_tag="OO468_003979"
     CDS             complement(3110..3466)
                     /locus_tag="OO468_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6260881.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3479..>3829)
                     /locus_tag="OO468_003980"
     CDS             complement(3479..>3829)
                     /locus_tag="OO468_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6260882.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1079 a         1027 c          734 g          989 t
ORIGIN      
        1 ttccatttga ctggctcact ttttatgatg atgccggtgc atttatctcc agcaccagac
       61 tttctatctc aacgccatac gttgcatttt tggtaatatc cgtcagcgtc agtgcattta
      121 gtcccactgc cagactgtct tttatggcct ggaatgccgg gccagtacga tgacgtagta
      181 tcactccggc tcagttgcac cactgaccac cacatcacct tctgctgcaa tcgcctgcat
      241 cagggtataa ggggttatgg ccaccggact accaaacggc tgccagccct ctttcagttt
      301 atgtgtcagc ttttccgcaa gatctgacgg cggcgccgcc ctgacaacat catagtgttt
      361 aaatgccatg gttctttcca acatctgaaa aataattctt taaaatacct gacatgtaat
      421 acagaaaaaa cacaaaacca taccttaaat aaaaacctga ttatcaagca gatatgcatg
      481 gataaactac aagacgagat ataaaccacc ctgcatttaa ataaacaata aacaacatca
      541 gaaaaataat tctgttctat ggtttaattc aaaaatatca tttatacttt tcagaacatc
      601 accagcaagg cataaacaag gaaactaaat gaagtggatt gtgattgata cagttatcca
      661 gccatcatgc ggaatatctt tttcagtcat atggagtaaa ataaaattaa taatctggta
      721 tcaatcggat gctttcttac ctcctgaaag tatatttaca ctgactcaca caggcatcat
      781 gctcaataac aaagtgctac ctgtaaccat ttacaacgta gtaccattca ataaaacatt
      841 ctggaattta atcaaaaaca gccaggaatg ccctacaaat acagataacg tattgaatga
      901 atgctttaat aaccgttgca ctctgcaaat atgcccttat ggactaaaac aacaaagtcc
      961 ataaggagtt tactcacatc tgacaaaatc aatataaaca gcccctccgg agaggggctg
     1021 gagagtggcg ctatgtgcca ttccatggtg ccgggtgcct cccggtgaat tcagtaccag
     1081 cacctgaatc cgcgattatc ccatatacct actcgctgat tgcccctccg cacaggggga
     1141 ttcaccatgc cagtttcttt taacaaactc cccgcaaacc agacaacagt caaccgcctg
     1201 aattgtgaag tatttaaaaa tttctcccgc taactgatac ccggctaaca gtctggcgtt
     1261 ttctttttca gcaacgggaa agcaacaacc accacacccg ccaccagcac accgtcagcc
     1321 ggcactgaca ttatccggct gctgcaatgc cattaacaaa aacagtaagc aatcactttt
     1381 taccgtaaca ggtgataatc cagatatgta tctaccccag atgagtaatc cgaagttcat
     1441 ccataccaca ggtcctggct attctgttgt actcctgaac aagagcaaat aattctgaat
     1501 tagcaaccat gaactcatcg caaaccctct gtatagcatc actattcaga ataataacgt
     1561 ctcttcccga aagacgatca ggagtacaga acaaaactgt caaacggctg aaggcctttg
     1621 ctcgtgctgc attgactata tcaatacgct gcctaaggat gaaacacccc gacgcctcat
     1681 caatattcac tctacccaca ccatatgaat gataaatact taatgctgaa aaaaccatta
     1741 gaccgtataa caaatactca atcaatactt aacagaactt ttatttttga caaatatata
     1801 atattttcaa caatatcctg agccaggtat atttcagtat aaggctctgc cggaaggaat
     1861 ctggaagaat gaatatggcg cgctgtactg gattcgaacc agtgaccgat tgcttagaag
     1921 gcaattgctc tgtccggctg agctaacaac gctgaatacc gataatggac cgccatcggg
     1981 gacccgcccc ccgcaccaac aaccctgtta tcgtgtcgtc tgctcttcct gataagctaa
     2041 tggcggtttg tgatggtggc ccttgctgga tttgaaccag cgacctggcg attatgagtc
     2101 gctcgctctc accactgagc taaagggccg ggagcagaat aataacggtc cgtaattaat
     2161 ttcgcaataa aaaaacccgc tcagcggcgg gttgtagaaa ctcttctaac gccaggcata
     2221 aaacgcccat cattatgacg aatttaccac agattccgga aaaatcaacc ttgttaccta
     2281 gttacctttt ttaactgccg ctcagcccat gcttcttcaa tatcaaaccg ggtcaccagc
     2341 gcatcataaa atttcttaac tgttttttcc catgacgcgc gtgttatctg gtttgtcacc
     2401 tcgcatatag cattaaatgc ctccgttgat ggtagtcttt catagccacg accaccacaa
     2461 cgctggcagt ctctgataac aggcatacca cgttttaccg actcttcacg atgaatggcg
     2521 acaccacgcc cacggcaatc cttacaggcg gtggaaacct caccctttcc gccacactcc
     2581 ggacaggcaa cttttaccac ctccctgact tttttccatt cttcccagta aaacggatac
     2641 acacctttcg tacactttgc ccataccggc ggcttaccat ccggatactg gatcttgttt
     2701 gtaaaaacta tgctttcaat aaatttttcc ccatagcaac aagggcactg ctttttactc
     2761 gctgcgctgc gggcataatc ctcaaaagcg tacgaagcca taatgcgcat cactaccggt
     2821 tttatttctg ccggaagttt tctcaacgcc gccacacgat cgcaccgact gagtgcataa
     2881 tctgccagta attctgttgc ccgcgccctg tcattcatac tgatgcccat tttccccagg
     2941 aacgcagaaa aacccatctc agcccgattc tgtgtcatgc cctgcgcggc catcacatca
     3001 gtgatactca gcgcatcttt tgacgttgag gccgatgcat cggtcaggcc aggggatttt
     3061 ggggagtagt atttcggtaa atcttccagt ttcatttttt gacctgctct taatgcatta
     3121 tggggtaaat cttcaccccc agacgtccac cagatactgg ctgaccacga acgatattga
     3181 tttcatcaaa ctgctcatcg tccattaaca ctcccgcatg cgtcagcgca tccagcggtg
     3241 ctttcaggat attgtccagg tcgcgacgac gcttatccgg tggctctgca atcaccttta
     3301 tcgccagcct tccggacagg cttaatttca gccgctgctg gcgaacaata agcgccacag
     3361 cccggcgata acgctttccc tcctccgaga taaaatatgt gctgccacgg cgtcgccagt
     3421 aagtgttcac cgtcggcggg taaggtaaaa ccaaatctat gagcatcagt cacctctttt
     3481 acccaagcac gccagttgca aaggcgtgat caagaaaacg aaaaattaaa tcaacctgag
     3541 aaccatgctt ttcttcgaac gccagaggat ccgcatgaag ctcgttgtga tgctcccgac
     3601 acagcggtag cgtgaaaata tcgtgagatt tggtccccat tccgccctga ccatgaccaa
     3661 tcaggtgatg gggatcgtcg gctggcttac cacaacacgc acacggctgt gtcttcaccc
     3721 agcgtttgta tttctcgtta acccagcggc gacgtttagg tcgtttcatg aaagattccg
     3781 gagactcagg atcaacggca atgctgacca ccgtcttttc ctgttgtgg
//
LOCUS       DAJTUP010000204         3801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTUP010000204 DAJTUP010000000
VERSION     DAJTUP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.207"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(36..800)
                     /locus_tag="OO468_003981"
     CDS             complement(36..800)
                     /locus_tag="OO468_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260883.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            995..1507
                     /locus_tag="OO468_003982"
     CDS             995..1507
                     /locus_tag="OO468_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR6260884.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            complement(1596..2066)
                     /locus_tag="OO468_003983"
     CDS             complement(1596..2066)
                     /locus_tag="OO468_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR6260885.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAGEL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(2063..2596)
                     /locus_tag="OO468_003984"
     CDS             complement(2063..2596)
                     /locus_tag="OO468_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR6260886.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(2662..3291)
                     /locus_tag="OO468_003985"
     CDS             complement(2662..3291)
                     /locus_tag="OO468_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260887.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(3295..3510)
                     /locus_tag="OO468_003986"
     CDS             complement(3295..3510)
                     /locus_tag="OO468_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR6260888.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          934 a          986 c          834 g         1047 t
ORIGIN      
        1 aaagcactct gttacgtgcc cgcatagtcc agaaactacg taaagccgga ttcagcgtcg
       61 gtttggatgt cagagtagaa gtcattgagg tcacgctctc cattgccatc atcaatacct
      121 gcatcacggc gaagacgatc agcctccaga gacaccttat cagtggcggc cttgcggtag
      181 gctgtatcag caaatatttt tcctaccgtc gcaagcgtgc caacgatgga ctcaatacga
      241 actgtattgc gcatcattgc tttctcggcg gcgctgatat tttcaatcag catcttcctt
      301 tcctggtccc cattagcatc ttccagcgac accaaccacc gaccaatatt ctctgcagcg
      361 acaaggttgt tagcacgaag gcgaaataat tcgtcctcga gcgtcaacgc tttagcgtcc
      421 tctatcacct catctttgag cagaaggcga cgggcataac caccgtgttt taatgcctgc
      481 tggttaccgg gttggaatgg gttagtcggg ggatcggtac gcattccgcg tatcggtttc
      541 gtatccggtg tagattctgt ttttagttgc gtactttttt gtgtaaggcc agtaatggca
      601 ggctttctgc tggtacgcac tttccttttt tgcgtaccat ttttgcaaac ctgcgtaccg
      661 ccactgcgtg cccaatcaag ctttttggcc ctcttcctga tagccccttc tgtaacgccg
      721 tatttctcgc ctatatcacg gaggctaagg actccggccc ggtatgccga ttcgatggcc
      781 tcccagtccg gttttgccat gattatgctt cctgtgattt actgttatcg ctgcctgtca
      841 caataggttg ctttaattct tcctcatcac tcactcctgc gacgctccgt ggcagggccc
      901 cccacaggag taccttgtaa atgtaaggtg tttaagccta aattaagtcg tatcacggtt
      961 aaatagcaaa gaaaaattca cagaaggaaa gacattgatg gagtggataa atgctttgat
     1021 gaactatttt gtacttcatc catatcgcct atgggggctt ctttttataa tagcgttcac
     1081 aaaatccact ttactcattt cggcgatatt gccaccagca tcaattatga tggccactgt
     1141 aattacagtc agcaagacga ctctttcacc atgggaagct ggaatcacgg tcatgagtgg
     1201 cgcatggtgc ggtactatcg ttaattacca tcttggtata ataattgggc atatccctca
     1261 acttgcatgt ataatatcga gccgttccaa tacaatagaa cgggttcgcc ttcagttgca
     1321 aaataactct gtgtcaatac ttttcacttc gcgctttatc gccgtgctgc gttacattgc
     1381 tcctttggtg gcaggaatgt tgcaacttca tccagtgaaa gtgtatacag tgagtctgat
     1441 ttcagctgct agctggtcag cactttatgt tggtagtttt agcttcgtgt tgcctttttt
     1501 tagctaaact gcccataaaa agctcttgcg aatccagctt cccccattcc tctgacaaca
     1561 gaatctgatt tgaaggataa tgagtaaaat taccgctacc ccccaaggca ttggatcctg
     1621 atgtactcct gaagcattct caatgctgtc tggtcgctga tgattccgtc ccggataccg
     1681 agaacgtttc gtccagcaac tggagagagt tcgacggtgg catcattgcc catgccggag
     1741 gcgctggagg tttcggctga ggatggcaca gggcattttc ctttgacgag cacccgacca
     1801 ccattatcaa gcttgcgccg aagagcatca ttttcagctt tcgcatcagc taactccttc
     1861 gtgtatttag catcgagtgc atcagcatca cgctggcgct gctgcatgtc agtaatggtt
     1921 gcgttcgcca gcttcagttc tccggcgttt ttgtcgcgct gctctttgta ggtaatggcg
     1981 ttatcacggt aatgattaac agcccatgac aggcagacga tgatgcagat aaccagagcg
     2041 gagataatcg cggtgactct gctcattgat ctatccccca acaggctaat gcgctttcct
     2101 ggtcacgacg aataacctgt ccatagcagt tatttgaacg tatgcggcaa tcgcgcccaa
     2161 catcttttat ccaccagcga atcgcctcgc atgcgccctt acgatcaccg gcattcagcc
     2221 gcttataaaa cgtcgacggg aaacacttac cggggccaat gttatagggg caaaatgacg
     2281 ctatacccgc tttctggggt tcggtcagag gtactttaat attgcgctcc acccatgcca
     2341 gcgccttatc acgttcaata gcgttaacct ggtcacattt ttccttcgac agcttcattc
     2401 ccggtatgac aggcttacca tccaccattg tggcaccacg acagatggtc catatgccag
     2461 aaccatcacg gtatgccgtt gtgtgattac cctctttttc gttcagaaac tggtcaagta
     2521 tctggggagc agacgcgcct gcggcaatca gcgccagaac agcagccgac aggccgtatt
     2581 tgatttttgc gctcatggat atttatcagg atttatcggc aacaaataac gagccaactt
     2641 atatacgcct tttaagataa gtcagccccg gctggatcca gtcatcaggc tctttcttaa
     2701 agggggagta ttgaaatcac gaagaagagc ctcccgcacc gcagcatcca tgtctgcacc
     2761 actggcaagt gcttcaatct ctgccgccac ctgcaaatac cccatgcaac ggccaatgcg
     2821 tttcataact ctggtgtttt cgtctttcag gcatctgata gcgagattaa taattttcag
     2881 gcagtgatga tgcgatgccc ggacaggttt tccgttctca taaacgacga actcaccctc
     2941 ttcacaggaa accctgaaag gccgatgggg ttcctggtct ttatccggtg cagacgcaat
     3001 aaaatcactt ttatgctctt cctctccggc actgttaacc gtaatgacgt actcaggccg
     3061 caggtaagcc gcctcttttt ctgacaatgc ggccggtaaa aacttttcct gcaaaaactt
     3121 cataaaagtc tcgtgagcga ctttgtggca gtaatcgtaa atcgccgcat aatgctcatc
     3181 gcggcgtttg ttttcatctt cagaactcac caacgccgac aattttttat taagctcagt
     3241 gatttcattt tccaggtgat tgaatcgctg attcatttct tcatgcttca ttatttactc
     3301 tccccgtgca gccttacgct tgtcttctct tattttgaaa tacagattcg tcagataagt
     3361 cagaagcccc agaagcagac ttcccagcac accaatcgca gcccactgtg atggcctgac
     3421 ctgatccaac cactgcaaaa accagtagcc ggcactgccg gcggaggtgc cgtaggcaat
     3481 gcccgttgaa attttgtcca tggatttcat agcctcacct ccgcaaataa cggatggcgt
     3541 agttttacac tgagaaatga aagggatttg aaaagaaaaa aacgcaaaag cgggcgaaac
     3601 gatatataca gtaaggaaag cactctatcc aacaaaccac ccacagttaa tcggaataaa
     3661 agcagagtgc ttatgaatga tcgcacgccc gaaggttagt atttctgcac agcaattttg
     3721 caaaaaaagg cgatcattca taacttaaac gtctttcagt cactccggga tttcccatca
     3781 tcgcagactg aaagactctg a
//
LOCUS       DAJTUP010000205         3538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTUP010000205 DAJTUP010000000
VERSION     DAJTUP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.208"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            169..1821
                     /locus_tag="OO468_003987"
     CDS             169..1821
                     /locus_tag="OO468_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR6260889.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(1832..2002)
                     /locus_tag="OO468_003988"
                     /pseudo
     CDS             complement(1832..2002)
                     /locus_tag="OO468_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2135..2254)
                     /locus_tag="OO468_003989"
                     /pseudo
     CDS             complement(2135..2254)
                     /locus_tag="OO468_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2328..3258)
                     /locus_tag="OO468_003990"
                     /pseudo
     CDS             complement(2328..3258)
                     /locus_tag="OO468_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          966 a          822 c          807 g          943 t
ORIGIN      
        1 gaattgtgtc ctagacataa agtccgtgaa gggaataagg ctcaggtcag cgaatcttat
       61 agtggcccgc agctctgact gcaataaggc cggatacaga gcagcaacta ctcgtcgatg
      121 tcagaacaac ggaagccttg caaacgggga tgcgttcata caggaacaat gataccgtta
      181 aaaaaaaaca ttacgctaat aatgttcacc ctgtcattac tgacaggaaa tcctgctata
      241 gcgtatgaaa cagataaagt atataaaata accgttctgc atacaaatga ccaccatgga
      301 cacttttggc gtaacaatca tggtgagtac gggttatcct cccaaaaaac tctggtggac
      361 aatatccggc agaaagtaat aaataatggc ggaagtgttc tattattatc tggtggtgat
      421 attaacaccg gtgtacctga atcagacctt cagaaggctg aacccgatat tcgtggaatg
      481 aatctaattg gttatgatgc aatggccgtc ggtaatcatg aatttgataa tcctctaaat
      541 atattacgtc agcaggaaaa atgggccaca tttcctttcc tttctgctaa catatatcaa
      601 aaaagtacag ggaggcgttt attcagtcca tggaaaattt tcatccgtca gaatttgaaa
      661 attgcagtca ttggcttgac gacagatgat actgcgaaaa caggaaattc tgaatatttt
      721 actgatattg agttccgtca acctgctgct gaggcgagga gtgtcattga tgaacttaat
      781 cagcaggaaa agccagatat tattattgcg gcaacacata tgggtcacta tgacaatggc
      841 gagtcaggtt cgaatgctcc tggtgatgtt gaaatggctc gcagtttgcc gactggctca
      901 ttagcaatga ttgtgggagg acattcacaa gctcctgtct gcatggccag cgataataaa
      961 aaacagtgga attatattcc ggggacaacc tgtgtaccag ataaacaaaa tggcatctgg
     1021 attgtacaag cgcatgagtg gggaaaatat gtcggtcagg ctgattttga attctgtaac
     1081 ggtacaatga aactggttaa ttatcagtta cacccagtta atctcaaaat gagaattacc
     1141 agagaagatg gaaaaacgga attttctttt tacacccccg aaattactga agatccacag
     1201 atgttatcct tactgacacc attccagaat aaaggcaaag cacaactgga tgtgaaggtt
     1261 ggggttgtga acggacgtct ggaaggggac agaagtaaag ttcgttttgt tcagaccagt
     1321 atgggccatc tgattctgag cgctctgaca gaacgtattg acgcagattt tgctgttgtc
     1381 agtggtgggg aaattcgaga ttccatagag agtggtaaca tcacatacaa agatatactg
     1441 aaagttcagc catttggtaa tacggtagta agtattgatt tgacggggaa agaagtagct
     1501 gactacctag ctacagtggc acagatgaaa ccggattccg gagcctaccc acaatttttg
     1561 aataccagct ttgtagtcaa aaaaggaaaa atcgaaatgc taaaaataaa aggtaaatcg
     1621 gttgacctta acaaaaaata tcgtatgaca acatttagct ttaatgctac tggtggggat
     1681 ggctatccac gtattgataa tagacccgga tacataaata ccggatttat tgatgctgaa
     1741 gtacttatcg aatatatccg gaaacattca ccgctggatg ctgcttcata tgaaccaaaa
     1801 ggtgaagtca gttggcagtg ataacgtaat tgacgatcat cacgtgtttt ttgctgacag
     1861 tcgctgtgac tttgtgaaag tagtctctgc gtatgtttgc gatatgggaa tgcagttgct
     1921 gtattttgtg tttctgcttc tgccagttgt tgctgaattt aaccttgcgg cttaactgat
     1981 gttggagcgc tgccagcttc ttcgtcaatt tcgctgatta aagtttattc attatggcat
     2041 gatctgcgta agcggctcgt cagaaccata ttgatattta ctaagagctc agatcaactt
     2101 tccagggcaa cagatcgcgt acccggtttg ccggtcagtc ctggatatgc tcaatgacgt
     2161 aacgcagcca tttttctggc tccacattgt tcagacggca tgtgccgatc agcgagtaca
     2221 acaccgccgc atgttcgcca ccgctatcgg aaccccgccg gagagagttt cactctccgg
     2281 ctgctcacgg aaccgtacgt gacactctcg cgtcatacgg ctcttgatca ctcattcgat
     2341 gcacgtagta aataccagtg ggcaaatagc ttcggatact gtttccttac cagcgccagc
     2401 tttcgctgag ccttccggtt cgaaagtctg tatttagact gcatccactt aagcaaacgt
     2461 gactgcattg cactccacag tcgatagctg aagtttctgg accagaactt tccatagtac
     2521 tcaatccagc ctctcactat cgcattgtag cgcctcgcaa aatccagcaa actctcagct
     2581 gttgagcgat gtatacgcca cttcttgatt gtttctgtta ttttgcacat tgctgcattt
     2641 gatgcacccg gcatacattt tctgtacaat tcgcctttga aattcttcag agtacgcact
     2701 ttgaagtcat atccgagagt ggcatacaac atcatcggcg tacctttcga agggaacgcc
     2761 gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat ttgagagcag
     2821 gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg ctccatccgg
     2881 catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc gtgcctgagt
     2941 atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaacga agcctttaat
     3001 atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg caatcgcctg
     3061 gtgtgcggat ttacccggtc gataaccata cgagtctgca tggaacaggg gttccagtac
     3121 cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg ccggaatacc
     3181 gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg cctgcggcat
     3241 ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa cattctcgtc
     3301 aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt ttactcgttt
     3361 ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt ttacagcagt
     3421 cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat ggggcaagcc
     3481 ctttgctccg gacccattac ggtccttcac cgctactacg gtttactccg tccctgta
//
LOCUS       DAJTUP010000206         3478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTUP010000206 DAJTUP010000000
VERSION     DAJTUP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.209"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..389
                     /locus_tag="OO468_003991"
     CDS             <1..389
                     /locus_tag="OO468_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR6260890.1"
                     /translation="HGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITEN
                     GSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGW
                     LTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO468_003992"
     CDS             complement(367..990)
                     /gene="tetR(B)"
                     /locus_tag="OO468_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6260891.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO468_003993"
     CDS             1072..2277
                     /gene="tet(B)"
                     /locus_tag="OO468_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6260892.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO468_003994"
     CDS             complement(2390..2983)
                     /gene="tetC"
                     /locus_tag="OO468_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6260893.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3116..>3478
                     /locus_tag="OO468_003995"
     CDS             3116..>3478
                     /locus_tag="OO468_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6260894.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEA"
BASE COUNT          945 a          639 c          713 g         1181 t
ORIGIN      
        1 accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca cgtacttgct
       61 atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt caacagcaat
      121 ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt catgaaatgg
      181 gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta gattggagtg
      241 aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt tatgaatcta
      301 aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa aaaggttatg
      361 ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata tgatcaattc
      421 aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga tagcttgtcg
      481 taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag cgacttgatg
      541 ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga gtgcatataa
      601 tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt cgagagtttc
      661 atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc gatgacttag
      721 taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc tttccccttc
      781 taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg cgtcgagcaa
      841 agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta gcttctgggc
      901 gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta atgcgctgtt
      961 aatcacttta cttttatcta atctagacat cattaattcc taatttttgt tgacactcta
     1021 tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga aatgaatagt
     1081 tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg ccttatcatg
     1141 ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc taaccacttt
     1201 ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg gcttggaaaa
     1261 atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg cgcatcgctg
     1321 gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg ccgtttgctt
     1381 tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga taccacctca
     1441 gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct tggtttaata
     1501 gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc cttttttatc
     1561 gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg tgaaaccaaa
     1621 aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa ttcggtatac
     1681 atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc gcaattgata
     1741 ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg atggaatagc
     1801 atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt ccaagccttt
     1861 gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct cggatttatt
     1921 gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt agttttccct
     1981 gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg agtgatgtct
     2041 atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag ccttaccaat
     2101 gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc actaccaatt
     2161 tgggatggct ggatttggat tattggttta gcgttttact gtattattat cctgctatcg
     2221 atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag tgcttagtta
     2281 tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata acccaagagg
     2341 gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt tatttatctt
     2401 tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta tcggcaagct
     2461 cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt aagtttttaa
     2521 gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg acaatggcgt
     2581 ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa ccaatgatag
     2641 gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa tcaagataat
     2701 gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt gttgtgatgt
     2761 aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt tttttatttt
     2821 taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc tcatctattg
     2881 aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc agtgattgat
     2941 aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat ttaaaataac
     3001 ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt aagaacattt
     3061 aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta ataacatcca
     3121 attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc tattatggat
     3181 tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag cgggttatac
     3241 caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg ctagctatat
     3301 tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa aaactatcct
     3361 tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc gatttaagta
     3421 catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat tggaggca
//
LOCUS       DAJTUP010000207         3404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTUP010000207 DAJTUP010000000
VERSION     DAJTUP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.210"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO468_003996"
     CDS             complement(68..592)
                     /gene="sat2"
                     /locus_tag="OO468_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6260895.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(687..1160)
                     /locus_tag="OO468_003997"
     CDS             complement(687..1160)
                     /locus_tag="OO468_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6260896.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1492..2028
                     /locus_tag="OO468_003998"
     CDS             1492..2028
                     /locus_tag="OO468_003998"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR6260897.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            2143..2469
                     /locus_tag="OO468_003999"
     CDS             2143..2469
                     /locus_tag="OO468_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6260898.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(2657..2896)
                     /locus_tag="OO468_004000"
     CDS             complement(2657..2896)
                     /locus_tag="OO468_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR6260899.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          942 a          753 c          717 g          992 t
ORIGIN      
        1 cttccctcat gatgtttaac tcctgaatta agccgcgccg cgaagcggtg tcggcttgaa
       61 tgaattgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc gctgtttcgt
      121 tcgagacttg aggtctagtt ttatacgtga acaggtcaat gccgccgaga gtaaagccac
      181 attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat cgtatgccaa
      241 ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc gcgactcctt
      301 tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg ttccatgttg
      361 agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag caagcagagt
      421 cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca cttctggtag
      481 atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca atgaaatggt
      541 tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaatcttc atatgacgcc
      601 taacgcctgg cacagcggat cgcaaacctg gcgcggcttt tggtacaaaa ggcgtgacag
      661 gtttgcgaat ccgttgctgc cacttgttaa cccttttgcc agatttggta actataattt
      721 atgttagagg cgaagtcttg ggtaaaaact ggcctaaaat tgctggggat ttcaggaaag
      781 taaacatcac cttccggctc gatgtctatt gtagatatat gtagtgtatc tacttgatcg
      841 atcaggcttt tgtatatctc cccaccacct gaaacaatga catgatccgt tattttcttt
      901 aggttggtta aagcatcttt aattgatgga aagatcaata cgttctcatt gtcagatgta
      961 aaacttgaac gtgttacgac cgcatacttt cggttgggta atgctcccat tgattcaaaa
     1021 gtcttgcgtc caaccaacag ccattggtta taggtaatag ctttaaacag gagctgttca
     1081 cctttggcac tccatggaat atcagggcca ttcccgataa ctccattctt cgatatagct
     1141 accattagtg atagtttcac aattcttcct cagaggttaa cattttatta gtaagcatgc
     1201 tcgttttgtc acccgctgat gcttaccgtt aattaatata atcacttgta ttaaatagac
     1261 tttctttaaa aatacaagac actctgttat tacaaatcgt gcatgccgtc tatcctgctt
     1321 gcacgatgca cattatctca cccaaagaac tcataacatt ttaatttatt tagcattaaa
     1381 ttaaaactct accggtaaac aagcatctct aggcgtaaaa aacaggcact tttttaaatc
     1441 ccagctagaa taggctgtat aggcagacag ttgcaagaca aggagcgttt tatgtctaac
     1501 agtccatttt taaattctat acgcacggat atgcgacaaa aaggttatgc gctgaaaact
     1561 gaaaaaactt acctgcactg gattaagcgt tttattctgt ttcacaaaaa acgtcatcct
     1621 cagaccatgg gcagtgaaga ggtcaggctg tttttatcca gcttagcaaa cagcagacat
     1681 gtagccataa acacgcagaa aatcgcttta aatgccctag cttttttgta caacaggttt
     1741 ttacaacagc cgttgggcga tattgattat atccctgcaa gcaagcctag acggctaccc
     1801 tctgttatct ctgcaaatga agtgcaacgc attttgcagg ttatggatac tcgcaaccaa
     1861 gttattttta cgctgctgta tggtgcaggt ttgcgcatta atgaatgctt gcgtttgcgg
     1921 gttaaagatt ttgattttga taatggctgc atcactgtgc atgacggtaa gggtgggaaa
     1981 agcagaaaca gcctactgcc cacgcgccta atcccagcaa taaaataact cattgagcaa
     2041 gcgcggctta ttcagcaaga cgacaactta caaggcgtag ggccatcgct gccttttgct
     2101 ttagatcaca aatacccttc tgcttatcga caagcggcgt ggatgtttgt ctttccctcc
     2161 agcacgctct gcaaccaccc gtataacggc aaattatgcc gccatcatct gcatgactcc
     2221 gttgcgcgaa aggcattgaa ggcagccgta caaaaagcag gcatcgttag caagcgtgtc
     2281 acttgtcata catttcgtca ctcgtttgct acgcatctat tacaagcggg gcgtgatatt
     2341 cgcactgtgc aagaactctt agggcataac gatgttaaga ccacgcaaat ctatacgcat
     2401 gtgttgggtc agcattttgc cggcaccacc agtcctgcgg atggactgat gctacttatc
     2461 aatcagtaaa agtagataaa tatctttatg tagccctgag tagcccctga tactttttat
     2521 ggtgaaaaag ataaacaatc atagagggga taaatatgag ccagttctac cattgttcag
     2581 ataagtttaa gcaagatact gtcaatcaca gtgaccattc atggtcatta cacttcctcc
     2641 agttgccaga caatgcctaa cataaaaacg caaaagccca aaagtcagag cttaggtgtt
     2701 ctaggcgttt tctaagcgct gaagaaatac gtcttaccgt agattgcgtg cgtttctgaa
     2761 gcacattttc cttatacatg gctttatccc aggcagcttc atcaagctct tggagtaaca
     2821 agccagtaat aatgcgacac tctcgggttt gtagcgagcc gctggtgaga tcggagttat
     2881 agcggaaagg ggtcatttgt aattatttgt gggtggagga tagttaactt attattgtag
     2941 ttggaagagg ctgctttcca aacattagca cctcgtaatg cttgttactg tatattaatt
     3001 tatgctctta gcgcatgtta atgggctatt acaatgcata atgtccacat caagataact
     3061 gtggcttaac acctgtacaa gcgaaaaaaa tcaatcacct gtccggaaat acgggatcac
     3121 tgtaaagtgt tggtgaggca gtctaggagg gggatttaat ttgggatact gatgaaagga
     3181 ttatgatcag ttggatatag gatcgtacta agctgagcgg caggttagct agattagtta
     3241 accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc gtattagctt
     3301 acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat ccttaaaaac
     3361 tccatttcca cccctcccag ttcccaacta ttttgtccgc ccac
//
LOCUS       DAJTUP010000208         3347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTUP010000208 DAJTUP010000000
VERSION     DAJTUP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.211"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            18..896
                     /locus_tag="OO468_004001"
     CDS             18..896
                     /locus_tag="OO468_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6260900.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            893..2284
                     /locus_tag="OO468_004002"
     CDS             893..2284
                     /locus_tag="OO468_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR6260901.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2281..2538
                     /locus_tag="OO468_004003"
     CDS             2281..2538
                     /locus_tag="OO468_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260902.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2633..2833
                     /locus_tag="OO468_004004"
     CDS             2633..2833
                     /locus_tag="OO468_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR6260903.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2826..3206
                     /locus_tag="OO468_004005"
     CDS             2826..3206
                     /locus_tag="OO468_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR6260904.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          725 c          969 g          768 t
ORIGIN      
        1 attctgaggt gacagcgatg atgacgttta acctgcgtga acaacaaaaa agactacagg
       61 cgcgaatgga tgagttacgg gcagagattg catttgctca gaagggcgaa aagccatggc
      121 cttatcgttc ctgcctgatg cgtgaaggtc gcggatattg cgaaaaacat ggcgaatatc
      181 acacgcatat actggtgtgg agcgatcgta atggcgagga cagagaagaa atttcatgct
      241 gccctgactg cttaatcgct gaggccaacg atttgaccat ggagctgtcg tccatcaagg
      301 cggaagagct gactgataac gccggaattg ccctgcgttt tcgggactgc gagtttgata
      361 attatctgga ggttaatcct ggcgcagcca gaaatcttgc ggcctgtcgc cgctatgcgg
      421 agaactggcc agatatgctg gagaacggta ccagtcttgt tatgaccggc agttgtggta
      481 ccggaaaaaa tcatctggct gtggctatgg caaaacacat catccgtaac tatctggcca
      541 gtgtggagat caccgacgtg atgcgcctta cccgtgctgt gaaaaattgc tggcggaatg
      601 acagcgaaaa aacagcggat gaagttattg agcgttatgc gtcaatggat ttgctgatca
      661 tcgacgaagt tggcgttcag tttggcagcg cggctgaaat ggctattttg caggaaatta
      721 tcaacgccag gtacgaaagt atcttgccca ccattctgat cagtaacctc tcaccggaag
      781 agttgtgggc gttcatcagt ccccgaattg ccgacaggat cacagacggg ggacgcaact
      841 ggttgtcgtt taactggccc agttaccgtt ctcgtatcag aggtgtggct gcatgacaac
      901 tccagtatgg cgtaacgatg accttgaagg cgctgtcatc ggcgcgttct ttctgcgcgg
      961 agcagatcct gaagtgatgg atattctggc cacactgcca gcggacgttt tttctgtacg
     1021 agcgtatcag gatatctata caggcatctg cagacaggcc cgtgtttcag gagtgattga
     1081 ccccgtgctg ttgtgtaatg agatgccgga acttgccccg gtgattactg ataccgggcg
     1141 taaaacctgg gtgaagtctt cactggagca ctatgttgca gcgttgcggc gcaatgccgc
     1201 actgcgcgat gcagaaaaaa cactgaatga ggcgctgcag aaattacgtg atgcgcatac
     1261 ctgtgaagca gctgaagatg ccctgaagga tgcgcagaac atgatggtca cactgtcgac
     1321 aggaaagggc gtcattcagc cggtacatat tgatgatgtg cttccggaag tggttgagcg
     1381 tgttgaatgc cggaatcagg ggctggagaa atccaggacg ttgatgaccg gtattgatga
     1441 actggacgca aaaacaggcg gtatggagcc cggagacctg gtattcattg ccgcccgtcc
     1501 ttcgatgggg aaaaccgaac ttgcgctgga catcatcgac aaggtgactg agcaggggca
     1561 tggtgtgctt ctgttcacca tggagatggc gaacatccag attggtgaac gtatggtgtc
     1621 tgcggctgga gggatgccag tatcacgcct gaaatctgtg gctcactttg aagatgaaga
     1681 ctggacgcgt ttctcacaag gggtggggcg gatgaccggg cgcaatatct ggatggtgga
     1741 ccaggcgaac ctggccattg atgagatatg cgcaacaacg aaacaccacc tgattaaata
     1801 tccggaaacg gcgctggtgg tggttgatta tctcgggctg ataaaaaccc gaaccacggg
     1861 gcgtcatgac cttgccgtgg gtgaaatctc aaaggggctt aaaggcctgg caaaatccgg
     1921 tggttttccg ttgattgcgc tgagccagct ctcccgcggt gtggagtcca gacccaataa
     1981 acgtcccatg aactcagacc tgaaaaattc cggagaaata gaggcggatg cagacatcat
     2041 tctgatgctt tacagggatg aagtgtacaa cccggatacg caggccaggg gcatcgcaga
     2101 aatcaatatc acgaaacaac gtaacggttc tctgggaacg atttaccggc gtttttataa
     2161 cggacatttt ctgcctgtgg accaggaaag tgcacgggtg ctttccactc ccatgaagcc
     2221 cggcaatccg cgcagataca gtaataaacg aactgacagc agtaagatgg agcgtttctt
     2281 ttgaacaacc agacaatgac ttttacccct gaacaattgc gtaaacacgc gcaggaaatg
     2341 ctgcgtcatg ctgaacaact cgaaaaaacg ggcataacaa aagatgctat ccgtaaagat
     2401 atggtacccg cgcttcggga actgatgcag gcgaagcatc gcgcacagaa agcagtagat
     2461 gagctggtgg actgtgtggc agagctggaa accagagtcg gaaagtttga aaaactggtg
     2521 caggaggcgc tgcgctgatg cgccatgagt ttgttttgcc ctggcttgtg cgcgatcatt
     2581 atgtccctgg tggaaagccc gggataaaaa tcaccgaacc ggagagtatt tgatgagcgt
     2641 caaaattcaa acgataccgg aactgcttat ccagacacga ggtaatatga ccgaagtttc
     2701 acgaatgctg aactgtaatc gcgccacggt aagaaaatat gctgaagata aagagggcaa
     2761 agggcacgcc attgttgatg gtgttctgat tgttcatcgt ggatgggaca ggggtaaaga
     2821 cagtgatgcg tgatattcag caggttcttg agcgctgggg cgcgtgggtg gctaataatc
     2881 atgaggatgt gacctggtcg tccattgctg ccggttttaa gggattaatc ccttcaaaag
     2941 taaaatcccg cccgcaatgc tgtgacgatg acgcgatggt tatatgtggg tgtatggccc
     3001 gccttaaccg gaacaacaac gatctacatg acttgctggt tgactactac gtgtttggga
     3061 tgacattcat gacgctggcg cggaagcatg gatgctcaga tggttatata ggaaagaagc
     3121 tacaaaaagc cgagggggtg gtggaaggaa tgttgatgat gttgggtgtc cggttagaaa
     3181 tggacaaata tattgaacat ccctgatggt aaggggcgac tttctagaaa atattttacg
     3241 tacgtaaaaa acgggatatt ctgttaagag tggttgcttc gccacacagc ttaaacccgc
     3301 ctccgggcgg gttttttatg cctgaaaaac ggtacaggac gttaaac
//
LOCUS       DAJTUP010000209         3312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTUP010000209 DAJTUP010000000
VERSION     DAJTUP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.212"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..140
                     /locus_tag="OO468_004006"
     CDS             <1..140
                     /locus_tag="OO468_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR6260905.1"
                     /translation="PLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            297..1046
                     /locus_tag="OO468_004007"
     CDS             297..1046
                     /locus_tag="OO468_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR6260906.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1068..1634
                     /gene="yieF"
                     /locus_tag="OO468_004008"
                     /gene_synonym="chrR"
     CDS             1068..1634
                     /gene="yieF"
                     /locus_tag="OO468_004008"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR6260907.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1688..3025)
                     /gene="adeP"
                     /locus_tag="OO468_004009"
     CDS             complement(1688..3025)
                     /gene="adeP"
                     /locus_tag="OO468_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR6260908.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3190..>3312
                     /locus_tag="OO468_004010"
     CDS             3190..>3312
                     /locus_tag="OO468_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR6260909.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEE"
BASE COUNT          919 a          855 c          817 g          721 t
ORIGIN      
        1 ttcctctccc gtttgacgaa agccagcaaa aaaagctgga agttcctttt accctgctgt
       61 ggcataaacg gaacagccat aatccgaaga tcgtctggtt acgggaaacc attaaaaacc
      121 tttacgcgtc gatggcataa ccgaatcgta tgaaacgcga cccaatttca caataaaatg
      181 taaaaaagtt gtaataagct tgtctgaatc gaacttttag ccgctttagt ctgtccatca
      241 ttccagtaaa tgattactct tgtattcata atggaccatt aagcatggag cgaaaaatgg
      301 cgactcactt tgcccgaggg attttaacgg aaggacatct gatttctgtt cgtctcccct
      361 cccagtgtca tcaagaagcc cgaaacattc cccctcatcg tcaaagccgt tttctggcgt
      421 ccagaggttt actcgcagaa ctgatgttca tgctgtatgg cattggcgaa ttgccggaaa
      481 tcgtcaccct gccgaaaggt aaaccggttt tcagtgataa aaatttgcct tcgttttcta
      541 tttcctatgc cgggaatatg gttggcgtgg cgttaacaac cgaaggtgaa tgtggcctcg
      601 atatggaact acagcgtgcg acgcgcgggt ttcatagccc acacgcgccc gataaccaca
      661 ccttttccag caatgaatcg ctatggatca gtaaacaaaa cgatcctaac gaagcgcggg
      721 cgcagctcat tacgctgcgc cgaagcgtgc taaaactaac cggtgatgtt ttgaatgacg
      781 atccgcgcga tctgcagctg ctgcccattg ccggacgcct gaaatgtgct catgtaaatc
      841 atgtagaagc gttatgcgac gcggaggacg tgctggtgtg gtccgtggcg gtcacgcccg
      901 cgattgaaaa gctcagtgtc tgggagttag atggcaaaca cagctggaaa agcctgccgg
      961 atattcacag ccgcgccaac aatcctacca gccggatgat gcgttttgcc cagctctcta
     1021 ccgtgaagtc tttttcgcca aattgataga caaccacagg agtcattatg tctgaaaaat
     1081 tgcaggtggt tacgttactg gggagcctgc gcaaaggctc atttaatggc atggttgcac
     1141 gtaccctgcc gaaaattgct ccggcgagca tggaagtcaa tgcgttacct tccattgccg
     1201 acattcccct gtatgacgct gacgtacagc aggaagaagg ttttccagca acggttgaag
     1261 ctctggcgga acagatccgt caggctgacg gtgtggtgat cgtcacgccg gaatataact
     1321 actcggtacc gggtgggctg aaaaatgcca tcgactggct ttcccgcctg ccggatcaac
     1381 cgctggccgg taaaccggtg ttgattcaga ccagctcaat gggcgtgatt ggcggcgcgc
     1441 gctgtcagta tcacctgcgc cagattctgg ttttcctcga tgcaatggtg atgaacaagc
     1501 cggaatttat gggcggcgtg attcagaaca aagttgatcc gcaaaccgga gaagtgattg
     1561 atcagggtac gctggaccac ctgaccgggc aattgaccgc atttggtgag tttattcagc
     1621 gagttaagat ctaaataaaa aacccgccag taatcatgca tggcgggttt ttaatgcgct
     1681 atcgatttta atgagcgtcg ataaatacaa tcttcaggat aaacagcagc gcaacgatga
     1741 ttacgcacgg gctaaggtca cgcagacgcc cggtaccaat cttcatcacg cagtaggaga
     1801 taaagcccag cgcaatacct tcggtaatcg aaaagctgaa cggcatcatc acggcggtaa
     1861 taaacgccgg aacagattca gtaagatcct gccagttcac gcgagcaaga cttgaggtca
     1921 tcaacacgcc aacgtagatc aacgcgccag ctgcagcgta gtctggcacc attcccgcca
     1981 gcggcgacag aaagataacc agcaggaaca gcagaccaac aaccactgcc gtcagaccgg
     2041 tacgaccgcc aaccgatacg ccggaagagg actcaatata agccgtaacg gaagaagtac
     2101 cgataaacga accggtcacg gaagagatac tgtcgacata cagcgcctgc ttcatgcgcg
     2161 ggaatttccc cttctcatcc gccagacctg ctttatcggt cacgccaatc agcgtaccgg
     2221 aggagtcaaa caagttgacc aacatgaaag agaaaatcac ccctgccagc ccgaggttaa
     2281 acgacccggc taaatctaca tgacccacaa ctgtcattac gctcggcggc gcagaaacga
     2341 tgccattgta gtgcacatca cccagcatcc agcccagcag cgtcgtcacc acgatagaaa
     2401 ccagcaccgc tgcgtgaatg ttgcgcgagg ccagaatagc aatgatgaaa aagccgagga
     2461 tacccagaag tacgctgtga gaagtcagat taccgatgct caccagcgtt tccgagttag
     2521 cgacaatcac acctgcgttt ttcagcccca tcatgccgat aaacaggccg ataccgctgg
     2581 taatacccac acgcagactt accggaatgt tagcaatcat ccagtaacga acgcggaaaa
     2641 tggtcaaaag aagcagtccg acagcgcccc agaagattgc gcccataccg atctgccacg
     2701 gcagccccat tgcctggaca accacaaaag cgaaaaaggc gttaagcccc atcgcaggag
     2761 ccagcgccac cggaaggttt gcaaacaatc ccatcataat gctaccgaaa gcagcaatca
     2821 gacaggttgt tacgaagacg gcactggtgt ccatgccagc aacgcccaga atttgtgggt
     2881 taacaaatac gatgtagacc atcgtcagga aggtggtgaa cccggcaatc acttcagttc
     2941 gtgccgtagt accatgttcg cgcagtttaa aaacacgttc cagcatcctc tgaccagaag
     3001 tctgggtggt atgttgatga ctcattatct atttccgaac aaggagggaa aatccgtcgc
     3061 tatcgtatac caaaatgcga caataggcgc gtttgtgaga gactttttta ttggatttac
     3121 ttatacggca acgattgcgt tgcgcaaatc ggctctacga aaacgttaaa ctgattaaaa
     3181 aggaaaggca tgtcccggat agaagcggta tttttcgact gcgacggtac gctggtcgac
     3241 agtgaagtca tttgctcccg cgcatatgta acgatgtttc aggaatttgg tattacgctc
     3301 gatcctgaag ag
//
LOCUS       DAJTUP010000210         3287 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTUP010000210 DAJTUP010000000
VERSION     DAJTUP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.213"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(57..260)
                     /locus_tag="OO468_004011"
     CDS             complement(57..260)
                     /locus_tag="OO468_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260910.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(262..417)
                     /locus_tag="OO468_004012"
     CDS             complement(262..417)
                     /locus_tag="OO468_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR6260911.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(620..1027)
                     /locus_tag="OO468_004013"
     CDS             complement(620..1027)
                     /locus_tag="OO468_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6260912.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILPEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1104..1331
                     /locus_tag="OO468_004014"
     CDS             1104..1331
                     /locus_tag="OO468_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6260913.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1315..1737
                     /locus_tag="OO468_004015"
     CDS             1315..1737
                     /locus_tag="OO468_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260914.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1815..2592
                     /locus_tag="OO468_004016"
                     /pseudo
     CDS             1815..2592
                     /locus_tag="OO468_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2599..3287
                     /locus_tag="OO468_004017"
                     /pseudo
     CDS             2599..3287
                     /locus_tag="OO468_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          834 a          726 c          902 g          825 t
ORIGIN      
        1 tatccgggta tcgcgcgtct gtttttgttt gcgaattcag tcaatgcgca tggtatttat
       61 accccgccaa gacccggtga atatcctgca cggccttctg ctttgtagaa ggcttcttcg
      121 ctatcataat cgccagattc cagcgcttct ttagccagcc agatgcgtgc cccagtgcct
      181 gcatttggct ccagttgctg gaggcgtttt gcatcttcta ggagtagagc gataacgtgt
      241 tttaattctg tctcgttcat tttactcacc tgaatgtctt cccaaccaac gacgtgcgcc
      301 agcttcggtt ttaaacgttt tgcttttggt atacgttatg gcggtgaatg tgccgtcctg
      361 gttggggaac acgccgtaca ccggagattc gttgttgcca agatcgatag tattcatgtt
      421 gaccccattt ccccttaacg ccggggtagc ggaactgttt gctgagaaca ccgtgcggtg
      481 tcttgatgaa taaaatttag aataacctaa ggagggtgat caagattttt gtgtagaaaa
      541 acctaagttt tttgatgtaa aaaacacaag tgtttgaaag tttgtgcttt ttattacagg
      601 gtgtggagaa aaaaggggat tatttgtttg cgcttctttt gcgagctttg agtagttctt
      661 caaaaagttt gttgaaattt tcaactcgag cacgcatctc tgacaacagg gccttttgct
      721 ctgactcagg cagtgcgtcg aacagttgaa gtaactcttt ttgatcttct ggcagaatgg
      781 ctggctgatt atccgggatc ggttcgcctg gttgtttatc ttcatcccca aaaagaagcc
      841 aagtcggtga gcactgaagc gcttggctca gtgcgaataa tcttttcccc gccggctgtg
      901 tttcatctct ttcccattga gaaattgtta cgtgagccac tttgaccagc ttacctaatg
      961 cggcctgaga cagttttaat tttttacgcc tatgtaagag gcgagcaccg aaggtttcgt
     1021 ttttcatatt agggaattct aatttttctt gacttaggtt tctctacggc ctggtttcct
     1081 taggaaaatc taaggagctc gatatgttga aaattgatgc tatagcgttt tttggcagca
     1141 aaacaaagct tgccaatgcc gcaggagtta ggctggcaag cattgctgca tggggggaac
     1201 tggttcctga aggtcgcgcg atgcgcctgc aagaggcatc tggcggggaa cttcagtacg
     1261 accccaaagt ttatgacgaa tatcgtaagg caaaacgacc tgggaaggtg attcatgaaa
     1321 atcaggcatg agcacatcga atcagtgctg ttagccctgg cagccgaaaa agggcaggcg
     1381 tgggtcgcta acgcaattac tgaagaatat ctgcggcagg ggggcggcga attgtctctg
     1441 gtaccaggca aggactggaa caatcagcag aatatctatc accgttggtt gaaaggtgaa
     1501 acgaaagcgc aaagggaaaa aattcagaaa ctgatccctg cggttctggc aattcttccg
     1561 cgtgagctgc gtcaccgact ctgtatcttc gataccctgg aacgccgtgc attactggcg
     1621 gcgcaggaag cgttgagtac ggcaattgat gcgcatgatg atgctgtcca ggccgtttac
     1681 cggaaagcac atttcagcgg tggtgggtcg cccggcgatt ctgtcgtagt gcattgattg
     1741 aaattaatcg tgccggattg ttttgttcgg tatcagttaa atgtaacgct gcgagcgtta
     1801 caaggtgaaa acaaatggct tcaaactgga taaagctcga ggttattacg ccggataagc
     1861 cggaaatatt caggcttgct gagattctga atattgatcc agatgccgca ttagggaagg
     1921 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
     1981 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
     2041 cgttaattca ggttggatgg ctggtcgaaa atgacggtgg gctttctcta cctaactttg
     2101 aacgtcataa cggaaaaagc tctaaaaaac gggcggttac aaacgagcga gtaacaaaaa
     2161 tacgcgaact gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt
     2221 taccagtgga agaggaagag gaagatctaa atactgatct ccccctaaat ccccctcgcc
     2281 aaaaacgagc gtctaaaaaa ttcgagccgg aggctattga gctgcccgat tggttgccgg
     2341 aaacactctg gcatgagtgg gtccggttca gacaggcatt gcgaaaaccg attcgaacgg
     2401 agcagggcga tacgggaact ggaaaaattc cgtcagcagg gttttacacc tgagcaggtg
     2461 attcgacaca gcatcgccaa tgaataccag ggcctgttcg cgccgaaagg tgttcggcct
     2521 gagacgttgc tccgacaggt taacaccgtc tcgttgccgg acagtgcgat cccgccaggc
     2581 ttcagggggt aacggaccat gaaaaatatt gcgacaggcg gcgttctgga acgcatccgc
     2641 cgactgaccc cgcaacatgt aaccgcccca ttcagaacgg tagcggagtg gcgcgagtgg
     2701 caacttgctg aaggccagaa acgtagcgag gagatcaacc gcctgaatcg ccagttgcgg
     2761 gtggaaaaaa ttctgaatcg ctcaggcatc cagccgttgc accgtaaatg ctcgtttgcg
     2821 aattaccagg tgcagaacga cggccagcga tacgcgttaa gccaggcgaa atccatcgcc
     2881 gatgaactga tgaccgggtg tacaaatttt gcgttcagcg gaaaacctga taccgggtag
     2941 aatcacttag cggcagctat cgggaatcgc ctgctgaaag acggtcagac agtgattgtg
     3001 gttaccgtgg ctgatgttat gagcgccctg cacgccagct atgacgacgg gcagtcaggc
     3061 gaaaaatttt tgcgggagct gtgcgaagtg gatctgctgg ttcttgatga aattggcatt
     3121 cagcgcgaga cgaaaaacga gcaggtggtg ctgcaccaga ttgttgatcg ccggacagcg
     3181 tcgatgcgca gcgtggggat gctgacaaac ctgaactatg aggtgatgaa aacattgctc
     3241 ggcgagcggg tgatggatcg catggtcatg aacggcgggc gctgggt
//
LOCUS       DAJTUP010000211         3267 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTUP010000211 DAJTUP010000000
VERSION     DAJTUP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.214"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..437)
                     /gene="ychE"
                     /locus_tag="OO468_004018"
     CDS             complement(<1..437)
                     /gene="ychE"
                     /locus_tag="OO468_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR6260915.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYH"
     gene            914..>3267
                     /gene="adhE"
                     /locus_tag="OO468_004019"
                     /gene_synonym="adhC"
     CDS             914..>3267
                     /gene="adhE"
                     /locus_tag="OO468_004019"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR6260916.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGL"
BASE COUNT          867 a          819 c          763 g          818 t
ORIGIN      
        1 ctgtgataac gcgtacccca gacgatggta gaactgatcg cccctggccc cgccatcaac
       61 ggcaacgcca atggcaccac accaatactt tcacgtaccg cggtttctga tttttcttgc
      121 ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg acatcgctat cgtcaccacc
      181 aggatgcccc cggcgatacg gaacgaatca attgatatac caaaaagttg tagaatcgtg
      241 tcgccgagaa aaagcgagat ccacaagata atggccacag acaggttggc tgtaaggtta
      301 gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc tgataaagac gggaataatc
      361 cctaccgggt tgaccagcgc aaataacccg atgaaaaatt tgaagtaaac gggaaaatca
      421 aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg gataatgtta gccataaata
      481 aggttgaaaa gacgcgctga caatacgcct tttgacagca tttttcacct cctaactact
      541 taaaattgct atcattcgtt attgttatct agttgtgcaa aacatgctaa tgtagccacc
      601 aaatcatact acaatttatt aactgttagc tataatggcg aaaagcgatg ctgaaaggtg
      661 tcagctttgc aaaaatttga tttggatcac gtaatcagta cccagaagtg agtaatcttg
      721 cttacgccac ctggaagtga cgcattagag ataataactc taatgtttaa actcttttag
      781 taaatcacag tgagtgtgag cgcgagtaag cttttgattt tcatcggtta agcaaatcat
      841 caccgcactg actatactct cgtattcgag cagatgattt actaaaaaag tttaacatta
      901 tcaggagagc attatggctg ttactaatgt cgctgaactt aacgcactcg tagagcgtgt
      961 aaaaaaagcc cagcgtgaat atgccagttt cactcaagag caagtagaca aaatcttccg
     1021 cgccgccgct ctggctgctg cagatgctcg aatcccactc gcgaaaatgg ccgttgccga
     1081 atccggcatg ggtatcgtcg aagataaagt gatcaaaaac cactttgctt ctgaatatat
     1141 ctacaacgcc tataaagatg aaaaaacctg tggtgttctg tctgaagacg acacttttgg
     1201 taccatcact atcgctgaac caatcggtat tatttgcggt atcgttccga ccactaaccc
     1261 gacttcaact gctatcttca aatcgctgat cagcctgaag acccgtaacg ccattatctt
     1321 ctccccgcac ccgcgtgcaa aagatgcaac caacaaagcg gctgatatcg ttctgcaggc
     1381 tgctatcgct gccggtgctc cgaaagatct gatcggctgg atcgatcaac cttctgttga
     1441 actgtctaac gcactgatgc accacccaga catcaacctg atcctcgcga ctggtggtcc
     1501 gggcatggtt aaagccgcat acagctccgg taaaccagct atcggtgtag gcgcgggtaa
     1561 cactccagtt gttattgatg aaactgctga tatcaaacgt gcagttgcat ctgtactgat
     1621 gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa cagtctgttg ttgttgttga
     1681 ctctgtttat gacgcagtac gtgaacgttt cgcaacccac ggcggctatc tgttgcaggg
     1741 taaagagctg aaagctgttc aggatgttat cctgaaaaat ggtgcgctga acgcggctat
     1801 cgttggtcag ccagcctata aaattgctga actggcaggc ttctctgtac cagaaaacac
     1861 caagattctg atcggtgaag tgaccgttgt tgatgaaagc gaaccgttcg cacatgaaaa
     1921 actgtccccg actctggcaa tgtaccgcgc taaagatttc gaagacgcgg tagaaaaagc
     1981 agagaaactg gttgctatgg gcggtatcgg tcatacctct tgcctgtaca ctgaccagga
     2041 taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg aaaacagctc gtatcctgat
     2101 taacaccccg gcttctcagg gtggtatcgg tgacctgtat aacttcaaac tcgcaccttc
     2161 cctgactctg ggttgtggtt cctggggtgg taactccatc tctgaaaacg ttggtccgaa
     2221 acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa aacatgttgt ggcacaaact
     2281 tccgaaatct atctacttcc gccgtggctc cctgccaatc gcgctggatg aagtgattac
     2341 tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc ctgttcaaca atggttatgc
     2401 tgatcagatc acttccgtac tgaaagcagc aggcgttgaa actgaagtct tcttcgaagt
     2461 agaagcggac ccgaccctga gcatcgttcg taaaggtgca gaactggcaa actccttcaa
     2521 accagacgtg attatcgcgc tgggtggtgg ttccccgatg gacgccgcga agatcatgtg
     2581 ggttatgtac gaacatccgg aaactcactt cgaagagctg gcgctgcgct ttatggatat
     2641 ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa gcgaaaatga tcgctgtcac
     2701 caccacttct ggtacaggtt ctgaagtcac tccgtttgcg gttgtaactg acgacgctac
     2761 tggtcagaaa tacccgctgg cggactatgc gctgaccccg gatatggcga ttgtcgacgc
     2821 caacctggtt atggacatgc cgaagtccct gtgtgctttc ggtggtctgg acgcagtaac
     2881 tcacgccatg gaagcttatg tttctgtact ggcatctgag ttctctgatg gtcaggctct
     2941 gcaggcactg aaactgctga aagaatatct gccagcgtcc taccacgaag ggtctaaaaa
     3001 tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc gcgggtatcg catttgcgaa
     3061 cgccttcctg ggtgtatgtc actcaatggc gcacaaactg ggttcccagt tccatattcc
     3121 gcacggtctg gcaaacgccc tgctgatttg taacgttatt cgctacaatg cgaacgacaa
     3181 cccgaccaag cagactgcat tcagccagta tgaccgtccg caggctcgcc gtcgttatgc
     3241 tgaaattgcc gaccacctgg gtctgag
//
LOCUS       DAJTUP010000212         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTUP010000212 DAJTUP010000000
VERSION     DAJTUP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.215"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO468_004020"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO468_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR6260917.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO468_004021"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO468_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTUP010000213         3128 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTUP010000213 DAJTUP010000000
VERSION     DAJTUP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.216"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..432
                     /locus_tag="OO468_004022"
                     /pseudo
     CDS             1..432
                     /locus_tag="OO468_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1016..1732
                     /locus_tag="OO468_004023"
     CDS             1016..1732
                     /locus_tag="OO468_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260918.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2284..2688
                     /locus_tag="OO468_004024"
     CDS             2284..2688
                     /locus_tag="OO468_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260919.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2751..3128
                     /locus_tag="OO468_004025"
                     /pseudo
     CDS             2751..3128
                     /locus_tag="OO468_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          879 a          638 c          708 g          903 t
ORIGIN      
        1 ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga gttactttaa
       61 agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg ctggcggaaa
      121 tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt gttgctcagg
      181 taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat tgtgctgggg
      241 aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct ggtgataaag
      301 gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact ggttgcctgt
      361 atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga atggaatgaa
      421 tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag cccgctccgt
      481 ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga ctgctgattc
      541 tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat ttaatgtttt
      601 gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa caagtaaaaa
      661 caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct cttgcaacac
      721 atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat gagccctaac
      781 tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta ccaactagcg
      841 ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc attctgcaga
      901 gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc ctagcatgaa
      961 aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt ttcttatgaa
     1021 tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat gtgtagggtg
     1081 ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc catggtgggt
     1141 tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca tagtaatggg
     1201 tatacttttt accggttggg ggattggtat tttatttttt cttcttataa agaaaagaag
     1261 taaaatacaa gacttgagtt tttttagact attttttgct attaccctac tgtttttccc
     1321 tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat cactttcatt
     1381 cattattagt tcattggtta tttcaataat aattcgtata tatggacgga tcttttctgt
     1441 atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa ttatggctgg
     1501 ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac ctcaattaaa
     1561 aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg ctcgttatga
     1621 ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct taatatttaa
     1681 tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt aataatcctc
     1741 tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat tgagctgtca
     1801 tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat gcaagaaagt
     1861 tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg gattatgtaa
     1921 tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc gagcattgca
     1981 ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac aaagaacaaa
     2041 aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac tgtctgtcac
     2101 tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg gatttgattg
     2161 tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc ggtcagcttg
     2221 ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa agtgacaatg
     2281 aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt acccgctgct
     2341 gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta tgcggagatt
     2401 aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag
     2461 agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg agtacagata
     2521 ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc gacactgcat
     2581 gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg gatgataatt
     2641 cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc ttacccagca
     2701 atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa
     2761 cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc agtgaagccc
     2821 tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc ctgtatgaat
     2881 ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct gagcgtgaaa
     2941 gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg ctatcctcca
     3001 aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt cggacaggaa
     3061 taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg gcggccttta
     3121 cccggtca
//
LOCUS       DAJTUP010000214         3094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTUP010000214 DAJTUP010000000
VERSION     DAJTUP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.217"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..689)
                     /gene="ipaH9.8"
                     /locus_tag="OO468_004026"
     CDS             complement(<1..689)
                     /gene="ipaH9.8"
                     /locus_tag="OO468_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR6260920.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSS"
     gene            complement(951..1046)
                     /locus_tag="OO468_004027"
                     /pseudo
     CDS             complement(951..1046)
                     /locus_tag="OO468_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1082..1387)
                     /gene="ccdB"
                     /locus_tag="OO468_004028"
     CDS             complement(1082..1387)
                     /gene="ccdB"
                     /locus_tag="OO468_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR6260921.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1389..1607)
                     /gene="ccdA"
                     /locus_tag="OO468_004029"
     CDS             complement(1389..1607)
                     /gene="ccdA"
                     /locus_tag="OO468_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR6260922.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2143..3094)
                     /locus_tag="OO468_004030"
                     /pseudo
     CDS             complement(2143..3094)
                     /locus_tag="OO468_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          771 a          749 c          749 g          825 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gcatgggatg ataatgggtt atcactaata
       61 tgtattgaac attcattcct cagattaaga atgctttccg ggatatgact tatctgattt
      121 ctatcaaaaa aatactctct gacaacggga ttatttccct cagaaaatgc tggtagctca
      181 gtgaggaaat tacgggtcgc tctgagattt ttaagagcct gtgggagcga tggtaaggag
      241 actatttcgt tatagctgat attcatagtc aataacgaat cgggtaaagc tggcaggttt
      301 tccagctcat tgtgttgtac ctgaagtgac tccagcgcag gaggtagcac gggcaattct
      361 gataatttat tgctggcgga atataatttt tttagcgtaa caggcagttc aggtaggtta
      421 gttaattgat tatatgatac attgagcagc gttatctgag ctggtaagtt gtcaggtagc
      481 gaggacagat ttaaacggtc cagtcgaagt tcatcggaat tattgataag acattcttta
      541 agtcgggaga cagcctcatc acgctcttca ccggggagcg cttgtttttc ccatttatcc
      601 catgctgaaa agtaatcagc atatgtaccg gaaatagtgt tataaaaaga attttggggc
      661 aatgaaaagt tattatttat cggtaacatg atgggcatcc agttaaagcc ggtggacaaa
      721 tggctttgtt aaaagaaata agtaggagtt tttatgaagt tcattccatg aaatgaactt
      781 cagcactttt ttattataat aaggataaac aaaattggct gtaaaaaaac gatactgttt
      841 cagttaaccg tgaatggcct gtttgcaccg tattgttctt gtttttcggt tagttaaaca
      901 tcgtacttca tatttgaacg gtaagcggct cgtcagaacc gtattgatat ttactgagag
      961 gtcagatcaa ctttccaagg caacagatcg cgtacccggt ttgccggcca gtcctggata
     1021 tgctcaatga cgtaacgcag ccattttgtc aacgccacga tttttgaccc tagcctgaca
     1081 tttatattcc ccagaacatc aggttaatgg cgtttttgat gtcattttcg cggtggctga
     1141 gatcagccac ttcttccccg ataacgaaga tcggcacact ggccatatca gtggtcatca
     1201 tgcgccagct ttcatccccg acatgcacca ccgggtaaag ctcacgggag actttatctg
     1261 acagcaggcg ggcactggcc agggggatca ccatccgtcg tccgggcgtg tcaataatgt
     1321 cactctgtac atccacaaac agacgataac gactctctct tttataggcg taaaccttaa
     1381 actgcatttc accagtccct gttctcatcg gcaaaagagc cgttcatttc aataaaacgg
     1441 gcgatctcag ccatcccctg ctgattttca gcctgccagc gttccgcacg cagacggcgg
     1501 gcctcttttt gcatggccgt atttaccagg cctgagatat tcacgtttgc ggatttaagt
     1561 aactgatagc tgtcactgtc tatagtgaca gtaatacgct gcttcatgat aagccctcaa
     1621 aaacatactt atgatatgta tatgagtata tattaatggt acaaaccatc aatatccata
     1681 aaaagtacac cattaattcg ctataagttt ttaatacaat tttttctgct gcgatttttt
     1741 tatactataa ttagcatact tataacaccg taagtaaatt acatgaaagc aggcacagcg
     1801 tgatccgatt tttctgcgta taacgatttt ccggaagtgt aatacggttg cgccccgggc
     1861 cgggcaccct tttctgttgt tgtgtacggg cccacagaga atggtaccgg aggcgggctc
     1921 agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta ctgacacggg tattggtgac
     1981 tggtgatgaa gtactgcccc gggtatgatt tcaggctgat tcaggttctg attctctgtc
     2041 agtccgcgtg gttgctggcc tggctgattt cctgttgtca ggcgttcaac aaccgactgc
     2101 aggttatagc ccggttcacg aacacctgac ggcaggtatc actcacccgg tgaaggtaaa
     2161 tccctcaaga ggcagcaact ctgccagtcg ctcctccggc cattccggca gacgcgtcag
     2221 gacgtccgtc agccaggcat gtggctccag accattacgt tttgctgttt ccagaaggct
     2281 cattatctgt gccgcccggt ttccggccat cagtgaaccg gcgaacagcc acgctttccg
     2341 tcccataaca accgggcgaa tggcacgctc gcagatattg ttgtccaggg gcacagctcc
     2401 gtcacccagg aagcagctca gttcatcgcg ccggttcaga atatagttaa tcgccttatt
     2461 taatggcccg tcgggcggac agcacggctc ctgctcttca agccaggcaa acaggtcatt
     2521 cactatcggc cgggagtacc gttatcgcca ctgccgtatt ttttccacag ggcgctcacg
     2581 gatgagtttc tcaatgcggt aaagacccgc gatttttctc agctccacgc ctgcacgggg
     2641 ctccctgctg gccttataca gatttgcgaa gccgcgccct gcgtgactcc agcaacaggc
     2701 tgatgtgatg ccggggtgat tatccgccag agatttgtac acgctgtatc cgtcactcac
     2761 cagcgtactg ccacaccagc actgcagcca ggtctcaggg tatctgaggg cccgtccggt
     2821 ctgcgagtca aaacagacga ccggcggacc gcttctttca ccgctgacgt aagcacacag
     2881 ccagccggag cagggtttac cgcctttccg ggtatcaagg agcctcaggg acgtttcatc
     2941 tgcatgaata acatcccgtg tcagaagctc atggcgtagc agtttcgcca gcggggccag
     3001 tgcagctccg gcaacaccga ccatatccgc cattgtactg acggggagct ccacacccat
     3061 tcgggagaaa atgtgctgct ggcgatacag gggc
//
LOCUS       DAJTUP010000215         3061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTUP010000215 DAJTUP010000000
VERSION     DAJTUP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.218"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..1913)
                     /gene="flhA"
                     /locus_tag="OO468_004031"
                     /pseudo
     CDS             complement(1..1913)
                     /gene="flhA"
                     /locus_tag="OO468_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(1906..3053)
                     /gene="flhB"
                     /locus_tag="OO468_004032"
                     /pseudo
     CDS             complement(1906..3053)
                     /gene="flhB"
                     /locus_tag="OO468_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          732 a          922 c          756 g          651 t
ORIGIN      
        1 acgggataga gcttcctgag tttgcgccag taaacgatcc gccagccctg gctccagtcc
       61 tcccccgccc tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga ggccaataac
      121 atggacttca tctttgccag gaaaccactg ctgggtaatc gcccgtccca acgccacgcg
      181 cacgacggcg cttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc
      241 gagaatggtg cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt
      301 atgcagtgtg gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc
      361 ctgggcgacg cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg
      421 ctggctaatg aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta
      481 cccctgaatc tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa
      541 tgccggatcg acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc
      601 cagccagcgc cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg
      661 atagcgggca ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc
      721 catctcctgg gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg
      781 ctggaaatcg accatcggga tcagtcgata acccacttcc attcccagag aatcttccag
      841 ttgtacatcg ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt
      901 gggttcggca ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc ccagcaatcc
      961 ggcagtgaac agcaaaaata ccaggttcgg cattccaggc accaggccga gtaaaccgag
     1021 cacggcggcg cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc
     1081 gccaacatcc tgatcggtgc tgacacgcgt aacgatgccc cccgcggcgg tagaaatcac
     1141 cagcgccgga atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc
     1201 cgcgtgtccc atgctcatgc cagttgcagc acgccgacca gcaacccgcc gacaacgtta
     1261 atgaccatga tgaggatccc ggcaatggca tcgccgcgaa caaacttact tgccccgtcc
     1321 attgagccgt aaaaatcggc ttcctgagtc acttcggagc ggcgtttttt cgcctcatct
     1381 tcaccaatca atccagcgtt aaggtcggcg tcaatcgcca tctgcttacc cggcatacca
     1441 tcgagaacaa agcgcgcacc cacttctgct atacgcccgg cacctttggt aatgaccata
     1501 aagttgatga tcacgagaat gacaaacacc acgataccga tagcgaaatt gccaccaacg
     1561 aggaagtgac cgaacgcttc gaccaccttc ccagccgccg ccgcgccggt atgcccttcc
     1621 atcaaaatga tacgggttga agccacgtta agtgccagac gcaacaacat agtaaacaac
     1681 agaatggtcg gaaacgcagc aaactcaagc gtgcgctggg taaacatcgc caccagcaac
     1741 accatgatcg acaaggcaat attgaaggta aacaacaggt cgagtatgaa tgcgggcagt
     1801 ggcagcacca tcatcgacaa gatcaacagg atcaaaatcg gtccggcaag gatctgccat
     1861 tatgtcgatt tcaggtttgc gggcaggcgc agcatcgcgg ccagattact catgggtcgg
     1921 tttctcgtta ataaaatcca gggctgccgg caccggaaga tgagtaggtg tacagggcgc
     1981 tgtccaccag ccagacgcca gcgtttcagt tgccagaccc aggccagcac ttccgccacc
     2041 gcggcgtaca gttgacccgg gatttgttga ccaatctccg catgtcgata cagcgctcgc
     2101 gccagcggcg gcgcttcaag cgtcgggacg ttattttcag cgccaatttc acgaatgcgc
     2161 agcgcgacca gccctgcacc tttagcgacc actttcggtg cgctcatttt gttttcgtca
     2221 tactgcaacg ctaccgaata gtgggtcggg ttattgacaa tgacatccgc tttcggcaca
     2281 tcggccatca tccgacgccg tgcggcagct cgctgcatct gacagatccg tcctttaaca
     2341 tgggggtcgc cttcgctttg tttgaactca tcacgaatat cctgccgcga cattcgcagc
     2401 tttttcaggt ggctgaagat ttggaaaaag acgtcaaatc ccaccattgg aatgacacca
     2461 agcaccacca gcagtgcgca tagccctacc aaatccatcg cattacccat ggcggtaatc
     2521 ggtgactcgg ccattaagcg catcatctgc ggccagtgat gccagagaaa aaaccccgtc
     2581 acgctgccaa ccaggatggt tttcaaaatt gccttaagca actccgcgct agtctgtgcc
     2641 gagaatatcc gtttaatgcc cggtagcggg ttgagtttgg aaaacttcgg ctgcaaggat
     2701 ttgccgctaa ataccagccc ccccagcata accggagaaa tgagcgccac cagcaccacg
     2761 ccgctaatca gcggtagcag cgccagcatg gcttctctga tcagcagaat aatttgcccg
     2821 aggatcagat tcgggtcttt gataatactg tgatcaaaat gcagcccagc ggagagcatg
     2881 cccgacaatc gacgggccag cgacacaccg ccaaaccaga taacactaac gcccaccagc
     2941 aaaatcagca gtgaggtcag ttcacgggaa cgcgggattt gcccctcttc ccgcgctttt
     3001 tctagtcggt gaggtgtggg ggcttctgtt ttgtcgtcgc tctcgtcaga cacgtcgcca
     3061 a
//
LOCUS       DAJTUP010000216         3023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTUP010000216 DAJTUP010000000
VERSION     DAJTUP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.219"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..2835
                     /locus_tag="OO468_004033"
     rRNA            <1..2835
                     /locus_tag="OO468_004033"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2931..>3023
                     /gene="rrf"
                     /locus_tag="OO468_004034"
     rRNA            2931..>3023
                     /gene="rrf"
                     /locus_tag="OO468_004034"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          797 a          668 c          942 g          616 t
ORIGIN      
        1 gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc
       61 cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg
      121 aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc
      181 gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga agcgtctgga
      241 aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg tgagctcgat
      301 gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct ccaaggctaa
      361 atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa agaaccccgg
      421 cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag cacgcttagg
      481 cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta gcaaggttaa
      541 ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg cagggtatag
      601 acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac taactggagg
      661 accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt gaaaggccaa
      721 tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct cgtgaattca
      781 tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca acccgatgca
      841 aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta acgtccgtcg
      901 tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt aagtgggaaa
      961 cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc atttaaagaa
     1021 agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg ctaaaccatg
     1081 caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt ctgtaagcct
     1141 gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg acataagtaa
     1201 cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc caacgttaat
     1261 cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat gggaaacagg
     1321 ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta tgttggccgg
     1381 gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc ggaaaatcaa
     1441 ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct gcttccagga
     1501 aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt
     1561 agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta
     1621 acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga gctgaaatca
     1681 gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa
     1741 agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg
     1801 caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa cggtcctaag
     1861 gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat gatggccagg
     1921 ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt gtacccgcgg
     1981 caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt gagccttgat
     2041 gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag ccgaccttga
     2101 aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt tgcggacagt
     2161 gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag gagcacgaag
     2221 gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc agcttgactg
     2281 cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg gttctgaatg
     2341 gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga taccgcccaa
     2401 gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat cctggggctg
     2461 aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc tgggtttaga
     2521 acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga ggggggctgc
     2581 tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc atgccaatgg
     2641 cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag cacgaaactt
     2701 gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg aagacgacga
     2761 cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta ctaatgaacc
     2821 gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag attttcagcc
     2881 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag cctggcggcc
     2941 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     3001 atggtagtgt ggggtctccc cat
//
LOCUS       DAJTUP010000217         2971 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTUP010000217 DAJTUP010000000
VERSION     DAJTUP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.221"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..798
                     /locus_tag="OO468_004035"
                     /pseudo
     CDS             1..798
                     /locus_tag="OO468_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            913..1254
                     /locus_tag="OO468_004036"
                     /pseudo
     CDS             913..1254
                     /locus_tag="OO468_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1237..2074
                     /locus_tag="OO468_004037"
                     /pseudo
     CDS             1237..2074
                     /locus_tag="OO468_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2124..>2971)
                     /locus_tag="OO468_004038"
     CDS             complement(2124..>2971)
                     /locus_tag="OO468_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR6260923.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKRALFLSAFTALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFY
                     TPQGS"
BASE COUNT          689 a          738 c          929 g          615 t
ORIGIN      
        1 ctgggccttg cccgtagcag ttatttttat caccgggctg cactgaaagc cggtgataaa
       61 tacgccacga tacgtaagat gctgacagat atctttaaca gtaattacca gtgttatggc
      121 tatcgtcgcc tgcatgcgat gctcaggcat gagggggggc ggctatcaga aaaggttgta
      181 cgcagactta tggtggagga acagcttgtc gtcagtcgta accgtcgtcg tcgctacagc
      241 tcatattgcg gagaaatcgg accggctccg gataacctta tcgccagaga ttttaaggcg
      301 gagcaaccta atcagaaata gctgacagat atcacggagt tccagctccc tgcaggtaaa
      361 gtctggctgt caccagtggt ggactgcttc gatggaaaag ttgtgagctg gtctctcagt
      421 acacgccctg atgctgaact ggtcaacact atgctggata gcgctgtcga aacgttaaat
      481 gctggcgaac gaccggtgat acacagtgac agaggtgggc attatcgctg gccaggctgg
      541 ctggaaagag tgaatgcagc aggtcttatt cgctcaatgt cccgtaaagg atgttcacct
      601 gataatgctg catgcgaagg ctttttcggc agactgaaaa cggaaatgta ttatgggcgt
      661 aaatggtcgg gcatcacgcc agaaaagttc atgcagcaag tggatgctta catcagatgg
      721 tataacgagc ggcgtataaa attatcgctg ggtgccgtca gccccaaaat gtaccgccaa
      781 caatgcgggc tggaatgata aagcagtcca ggaaatcgtc cgcatccccc cttcaccatt
      841 tggattgtcc gctccaccca acatgttgtt tccttaaggt tcctacacca caaaggaaac
      901 tctcatgctg cgcagtggat ggctgacgtt gccgacaggc gggaacttcg ccagttcagg
      961 cagacaccgg aacagcgctt cacgcaggaa caggagcatc tgcagccgct gctgggtacg
     1021 gacttcgata tccgtcatgt ctcctgggat ggctatatcg aggttggcgg gaaccgttac
     1081 agtgtgccgg aaagcctgtg tggtcagctg gtatcaatac ggatctcgct ggatgatgaa
     1141 ctgcggatct acagtaatga gcaacaggta gcatcgcacc gtctctgttc ggctgcatac
     1201 ggctggcaga ccgtgccgga gcatcacgct ccactctggc agcaggcttc ggagcgcatg
     1261 gccgaatggc tgggggagat acagaaacgg gtaatcactg tctgtgaccg ggaagcggat
     1321 atctggcatt acctgtatta taaagtcagc cacggacagc gtggtgcgtg ctgcacagaa
     1381 tcgccggctg gaagaggcac ccggcaagct cttcgaactg ccggaagtcc tggcaaccgc
     1441 aggaagccac acgctgaatg tgatgcaaaa aggcgggcgg gcagcccgtc aggcccggat
     1501 gttcatcagt tacagcgaag tcagcataaa aaatcccgac aacagcggcc aggcgctccc
     1561 gctcacgtat gtctgttgcc gggagcaggc agaggacggt gcctgctggc atctgctgac
     1621 gtcaggaaaa gcggcgagtg ccgccgatgc acgacgtatt gtcagccatt acgagcgacg
     1681 ctggctgaca gaggaatacc acaaggcgtg gaaaagtggt ggtacatgga atcgctgcga
     1741 atgcagaccc gggataacct tgagcgcatg gtggttatcc tggcgtttat agcggtacgg
     1801 gtgctgggcc tgcggcaggg aggtgtcagt gaagaaacac aaaatgacag ctgtgagaaa
     1861 atactgacgc cgacagagtg gaaactactg tgggttaagc tggaaggaaa gccgcttccg
     1921 gtgcaggcac cgaccctgaa atgggcctgg ggcgatggca tgacagcaaa cgcacaggtc
     1981 gtcccggttg gggcgtcatg tgggatggct ggtcagactt caggatatgg ttgagggtta
     2041 cctggtgatg aagtctcttg atcaggagct ctgatcaaga gacagcagct taggggggtg
     2101 cccctattaa gttgtcaagc atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc
     2161 atcatggcga acagaacgtc gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt
     2221 cgttttccct gactcatttt gcgggtgtag taagccctgg agagcggatc cctgagcgcg
     2281 gtgaaggccg acaggaacaa cgcccgtttg agagctttat taccccgtcg cgagggatgc
     2341 tcaccgcgta ttgacgagcc ggatcgccga gttaccggcg caaggccagc ataagcagcg
     2401 agatgtgcgg cagaggcgaa ggcgcggcag gcgacctcgg tgaggagtct ggctgcggtc
     2461 ctgacaccga ctccgggcat actggtcagg accgggtaaa gagggtgagc aagaactcgc
     2521 tgttctacct caagcgccac ctcgtctctt tgcttacgca gcgtgatgag ctggagtgcc
     2581 agacgtggca gtactacggc agcggcattc gtgccgggaa cgacgacggt ttgttcggcc
     2641 agtgcctgag ctatgtctgc tgcaaggcgt ttacccagac gaggcgcaag tttgcagagc
     2701 tgggctgcca gcttcttctc acccagcgaa gcgagttttt ctggtgaggg atatcgctgg
     2761 agaagatcga gtaccgccgg gtgctcaagt ctcggaccga gaacgcgctc cggtgccgga
     2821 tgtatctggg tcagaaggcc gcggatacgg ttgctggcct gcgttgtctg tgcggcaaga
     2881 tcatcatcga agccgcagag catggagagt tcggcgattt gctcgtcagc cagtttcagc
     2941 gtgcgtagcg cgtgaggcag ggtacgggca g
//
LOCUS       DAJTUP010000218         2954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTUP010000218 DAJTUP010000000
VERSION     DAJTUP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.222"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..96
                     /locus_tag="OO468_004039"
     CDS             <1..96
                     /locus_tag="OO468_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IIC forms the translocation channel for the sugar
                     uptake; involved in N-acetylgalactosamine transport;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR6260924.1"
                     /translation="QDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            135..938
                     /gene="agaC"
                     /locus_tag="OO468_004040"
     CDS             135..938
                     /gene="agaC"
                     /locus_tag="OO468_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR6260925.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            928..1719
                     /gene="agaD"
                     /locus_tag="OO468_004041"
     CDS             928..1719
                     /gene="agaD"
                     /locus_tag="OO468_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR6260926.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1720..2475
                     /locus_tag="OO468_004042"
     CDS             1720..2475
                     /locus_tag="OO468_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR6260927.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2876..>2954
                     /locus_tag="OO468_004043"
     CDS             2876..>2954
                     /locus_tag="OO468_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR6260928.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGE"
BASE COUNT          760 a          668 c          698 g          828 t
ORIGIN      
        1 caggatctcg cggacttacg ttttattaaa caacgtggcg tgaatgtttt cattcaggac
       61 gtccctggcg atcaaaaaga acaaatccct gactaaatct aaaatcgcct taatattggt
      121 ttgaggtaat aaaaatgcat gaaataaccc tacttcaggg attatcccta gcggcgttag
      181 ttttcgttct ggggattgat ttttggctgg aagccttatt tttattccgc ccgataatcg
      241 tttgtaccct aactggcgct attctcggtg atattcagac tggcttaatt accggtggtc
      301 tgacagagtt ggctttcgcc ggattaaccc ctgcaggtgg tgttcagccg cccaacccga
      361 ttatggcggg tctgatgacc accgtcattg catggtctac gggcgttgat gccaaaacag
      421 caattggtct tggcctgccg tttagtttgt taatgcagta cgtcattctg ttcttctatt
      481 ccgctttctc attatttatg accaaagccg ataaatgcgc gaaagaggcg gatacggcag
      541 cgttttcccg gcttaactgg acaacgatgc tcatcgtcgc ttcagcgtat gcggtgattg
      601 ctttcctctg tacttacctg gcacaagggg cgatgcaggc gctggtgaaa gcgatgcccg
      661 cctggttgac ccacggcttt gaagtggctg gcggtattct gcctgccgtt ggttttggct
      721 tgctgctgcg cgtaatgttc aaagcgcaat atatccctta cctgatcgcc ggtttcctgt
      781 ttgtttgcta catccgggtc agcaacctgt tgccggttgc cgtactgggc gcaggctttg
      841 cggtgtatga gtttttcaat gcgaaatccc ggcagcaagc gcaaccgcag cccgttgcca
      901 gtaaaaatga agaagaggac tacagcaatg ggatctgaaa tcagtaaaaa agatatcacc
      961 cgtctgggct ttcgttcgtc gctgctgcaa gcgagcttta actacgaaag gatgcaggcg
     1021 ggcggtttta cctgggcgat gttgccgatc ctgaaaaaga tttataagga cgacaaaccg
     1081 ggcttaagcg cggcgatgaa agataacctc gaatttatta atacccaccc gaatctggtc
     1141 ggattcctga tggggttatt aatttcgatg gaagaaaaag gagaaaaccg cgacaccatt
     1201 aaaggcctca aagtggcact gtttggccca atcgccggga ttggcgatgc gattttctgg
     1261 tttactttgt tgccgattat ggcgggaatt tgctcatcat ttgccagcca gggaaacctg
     1321 ctggggccga ttctattttt cgccgtttac ctgcttatct ttttcctgcg cgtcggctgg
     1381 acccacgtcg gttattcagt cggcgtgaag gcgatcgata aagtgcgaga gaactcgcag
     1441 atgattgctc gctcggcaac catcctcggg atcacggtaa tcggcgggct gatcgcttcg
     1501 tatgtgcata ttaacgtggt gacatcgttt gccatcgaca gtacccacag cgtcgcgctg
     1561 caacaggatt tcttcgataa agtcttcccg aacattttac cgatggccta caccctgctg
     1621 atgtattact tcctgcgggt gaaaaaagcg catccggtgc tgttaatcgg cgtgactttt
     1681 gtgctctcta ttgtttgttc cgcattcggc attttgtaaa tggaacgagg cactgcttct
     1741 ggcggtgcct ctttacttaa ggaatttcat cctgtgcaaa cccttcagca agttgaaaac
     1801 tatacggcgt taagtgaacg agccagcgaa tatttattgg ctgtgatccg tagcaaaccg
     1861 gatgccgtga tttgcctggc gaccggagcc acgccattac tgacgtatca ttatctggta
     1921 gaaaaaatcc accagcagca ggttgatatc agccagctca ccttcgtgaa gctcgacgaa
     1981 tgggtggatc tgccattaac gatgcctggc acctgcgaaa ctttcctaca acagcatatc
     2041 gtgcagccgc tggggctacg tgaagaccag ctcatcagct ttcgctccga agagataaat
     2101 gagacagagt gcgaacgggt aacgaacctg attgcgcgca aaggcggtct ggatttatgc
     2161 gttctcggat tggggaaaaa cggtcatctt gggctgaacg aaccgggaga aagcctacaa
     2221 ccggcctgcc atatcagtca acttgatgcc agaacacagc aacatgagat gttaaaaacc
     2281 acgggtcgcc ccgtgactcg tgggatcacc ttaggcctga aggatattct caatgcccgc
     2341 gaggttttgt tattggtgac tggcgaagga aagcaggatg cgacagaacg ttttctcacg
     2401 gctaaagtct ccaccgctat cccggcttca tttttatggc tgcacagtaa ctttatttgt
     2461 ttaattaata cctaaccaga tgaaaaattt gctcaaaatt tttaaattaa cttattcaac
     2521 agtcacgcat tatattaaat aacatttgac tgggttgaac ataacgccaa tagcaaaagg
     2581 agttatgttc aacaatttaa ttttagatat acatataact tgttctatta ataaatcacc
     2641 tcgtattctg actcggcccc tggcgcagca aagtcaggca tttatactct ttttaacaca
     2701 tagaaaattc atatcaatat aataaaaaca taaaatattt gcatatacat ataatcttaa
     2761 agtttagtct atttaatgtt caatgaaata tttctacctg tataatcttt aaagatgttg
     2821 aacatatatt cacattaaat atgattatgt acttgttaca aggataaggt tataaatgaa
     2881 taaagttaca aaaacagcta ttgtgggctt acttgcgctt ttcgcaggta atgccgctgc
     2941 aaccgatggt gaaa
//
LOCUS       DAJTUP010000219         2922 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTUP010000219 DAJTUP010000000
VERSION     DAJTUP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.223"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(140..670)
                     /gene="cybB"
                     /locus_tag="OO468_004044"
     CDS             complement(140..670)
                     /gene="cybB"
                     /locus_tag="OO468_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR6260929.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            859..1859
                     /gene="gap"
                     /locus_tag="OO468_004045"
                     /pseudo
     CDS             859..1859
                     /gene="gap"
                     /locus_tag="OO468_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            complement(1901..>2922)
                     /gene="aldA"
                     /locus_tag="OO468_004046"
     CDS             complement(1901..>2922)
                     /gene="aldA"
                     /locus_tag="OO468_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR6260930.1"
                     /translation="RALGVTTGILPWNFPFFLIARKMAPALLTGNTIVIKPSEFTPNN
                     AIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAGNPKVAMVSMTGSVSAGEKIMATAA
                     KNITKVCLELGGKAPAIVMDDADLELAVKAIVDSRVINSGQVCNCAERVYVQKGIYDQ
                     FVNLLGEALQAVQFGNPAERNDIAMGPLINAAALERVEQKVARAVEEGARVALGGKAV
                     EGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVVAFDTLEDAISMANDSDYGLTSSIY
                     TQNLNVAMKAIKGLKFGETYINRENFEAMQGFHAGWRKSGIGGADGKHGLHEYLQTQV
                     VYLQS"
BASE COUNT          777 a          744 c          663 g          738 t
ORIGIN      
        1 aagccagatg gacgtatggg caaggattat ggttttattt gtcatacaaa taaatataat
       61 aggcgcttcc gatgtagacc cgtattcttc gcctgtacca cgggtcggtt ttagtacagg
      121 cgttttcttt aaatatcctt caggaacgtt tacgcggcat catgcgtaga agtgtgttgt
      181 ccttccagaa atagtggtgt gccagtgcag ccgcagcgtg caacccgatg acaaaatatc
      241 ccagattcgc cagcgtctca tgccacgact ttaagctatc aacccgttcg aaattggcct
      301 ctgaagcgta aggcatcgtc aaaccaaacg caaaccacgg gttgccccgg ttatacatca
      361 tcaccaaacc aatcactggc agcgcaataa acaggagata aatcaccaaa tgtcccaaat
      421 gcgccagtcc cgtcatcatc ggtttcggct taggtataat cggcggggtt gggtatttca
      481 gtcttaacaa aagacgaacc accatcagca cgagaattga gatgccacag gaaacatgaa
      541 tcatgttgat gagcggccga tcgctacgtg ggaagaaacc acgaaactcc attgcgcaat
      601 acgctgcgat aaccagcaaa aagaccagcc agtgaatgct gatttgtaac cttgagtatt
      661 tattttccat aacatttcct gctttaacat aattttccgt taacataacg ggcttttctc
      721 aaaatttcat taaatattgt tcacccgttt tcagtacgtt acgtttcact gtaagaatgg
      781 agattgccca tcccacaatc ctggtctaag cctggaaagg atcaattttc atccgaacgt
      841 tcctgacagg agaaaaccat gagtaaagtt ggtattaacg gttttggtcg tatcggtcga
      901 ctggtgttgc gtcgtttact tgaagtcaaa agcaacatag acgttgtcgc tattaatgat
      961 cttacttccc caaaaattct cgcctatctg ctgaaacatg attcaaacta tggtccgttc
     1021 ccctggagcg ttgattttac ggaagattca cttatcgttg atgggaaaag tatcgcggtt
     1081 tacgccgaaa aagaggctaa aaatattccg tggaaagcga aaggggcaga aatcattgtc
     1141 gaatgtactg gcttttatac ctccgccgat aaatcgcagg cgcatcttga tgctggtgcg
     1201 aagaaggtgt tgatttccgc tcctgccggt gaaatgaaaa ccatcgttta taacgttaat
     1261 gacgacactc tggatggcaa cgacaccatt gtttccgtgg cgtcatgcac cactaactgc
     1321 cttgcgccga tggccaaagc cttgcatgac agtttcggga tagaagtcgg cacgatgacg
     1381 accattcatg cctatactgg cacccagtca ctggtggatg gcccgcgtgg taaagattta
     1441 cgtgcttcac gcgcagcggc agaaaatatc attccccaca ctacgggggc ggcaaaagcc
     1501 attggtctgg tgatcccgga actgagcggc aaactgaaag gtcatgcgca acgcgtgccg
     1561 gtgaaaacag gttcggtcac tgagctggtg tccattctcg gaaaaaagtg actgccgaag
     1621 aggtgaataa cgcacttaaa caagcaacca ccaataacga gtcatttggt tataccgatg
     1681 aagaaatagt ctcttccgat atcattggca gccatttcgg ttcagtgttt gatgccacgc
     1741 aaacggaaat taccgccgtg ggcgatttac aactggtgaa aacggtcgcc tggtacgata
     1801 acgaatatgg cttcgtcacg cagcttattc gcaccctcga aaaattcgct aaactctgac
     1861 gcgcacaggc ggaggaaaaa acctccgcct ctttcactca ttaagactgt aaataaacca
     1921 cctgggtctg cagatattca tgcaggccat gtttaccatc tgcgccgcca ataccggatt
     1981 tacgccatcc ggcgtggaaa ccttgcatag cttcgaagtt ttcacggttg atgtaagttt
     2041 caccaaactt cagcccttta atggctttca tcgcgacgtt cagattttgg gtatagattg
     2101 atgaggtcag gccgtaatca ctgtcattag ccattgagat agcatcttcc agcgtgtcaa
     2161 atgcgacaac tggcagcacc gggccaaagg tttcctcatg cataatcgac atttcctggc
     2221 gaacatccag cagcaatgtc ggcggataat aatatccttt cccctctacc gctttgccac
     2281 ccaacgccac tctcgcccct tcttctactg cgcgcgccac tttttgctca accctttcca
     2341 gcgccgcggc gttaatcaac ggccccatcg caatgtcgtt gcgttcagcg gggttaccaa
     2401 attgaaccgc ctgcaacgct tcacccagca gattgacgaa ctgatcataa atgcctttct
     2461 gtacataaac acgttctgca cagttacaca cttgcccact attaatgacg cgtgaatcaa
     2521 cgatggcttt gactgccagt tcaagatcgg catcgtccat tacgatagcc ggtgctttac
     2581 cacccaattc cagacacact ttggtgatgt ttttcgccgc agtcgccatg atcttctcac
     2641 ctgcagagac gctgcctgtc atactgacca ttgcgacctt tgggttaccc gccagttctt
     2701 gcccaacggt ttcaccacgc cccagtacaa ggttaaacac gccgcgcgga aggcctattt
     2761 catcgacgat tttggcgaat gcaatcgcat tgtttggcgt aaattcactg ggtttaatga
     2821 cgatggtatt accggtcaaa agagcgggag ccattttgcg ggcaatgagg aagaacggaa
     2881 agttccacgg cagaatgccg gtagtcacac caagcgcacg tt
//
LOCUS       DAJTUP010000220         2882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTUP010000220 DAJTUP010000000
VERSION     DAJTUP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.224"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..228
                     /locus_tag="OO468_004047"
     CDS             <1..228
                     /locus_tag="OO468_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR6260931.1"
                     /translation="YVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALS
                     EKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            238..876
                     /locus_tag="OO468_004048"
     CDS             238..876
                     /locus_tag="OO468_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR6260932.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            879..2042
                     /locus_tag="OO468_004049"
     CDS             879..2042
                     /locus_tag="OO468_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR6260933.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2126..>2882
                     /locus_tag="OO468_004050"
     CDS             2126..>2882
                     /locus_tag="OO468_004050"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR6260934.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAI"
BASE COUNT          652 a          690 c          813 g          727 t
ORIGIN      
        1 tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga ctatttcgcc
       61 tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag actttatatg
      121 cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt tatgggaacc
      181 gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg gacgatcatg
      241 gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat cagtcgcatt
      301 ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa atacttaacg
      361 ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga tagtagcgat
      421 gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat tgatgacggt
      481 aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt ttatgccgat
      541 gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg ctactacgac
      601 aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg ggttattcgt
      661 aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc ttcccgcccc
      721 gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa aaaagagtct
      781 tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg cggctatggt
      841 cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac aacgttcctg
      901 tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat atcatcgaca
      961 atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag attgaagagc
     1021 agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg gatcgcgcgg
     1081 ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg gatgtgatcg
     1141 ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt gcctggtgtg
     1201 gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg ttgtatgagt
     1261 cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt attccgcgtg
     1321 atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt gagctttaca
     1381 gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg agtaccgtgc
     1441 tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc tacattgaag
     1501 atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc atccagcgtg
     1561 catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg ctgctgcgca
     1621 agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat aaagggctaa
     1681 tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc tggtttgagg
     1741 gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc tactcgcgcg
     1801 aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac gctgatggtg
     1861 attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga tttggcgata
     1921 gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg gggatccgtg
     1981 aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac attgctggat
     2041 aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat actaatggtg
     2101 actgccattg atggagggag acacagtgca ctggcaaact cacaccattt ttaatcaacc
     2161 tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg aagcggtaac
     2221 gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc agttaggaac
     2281 ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac tgttgcgcta
     2341 cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc acctgctgat
     2401 gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg ctcgctccgg
     2461 cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg ctgggtcgtt
     2521 atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt tacccacgcc
     2581 gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact tattgccagg
     2641 tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg gcggttccga
     2701 tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc ggctggtggg
     2761 gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg cgcagaccgc
     2821 gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac gcaacgcgat
     2881 tc
//
LOCUS       DAJTUP010000221         2813 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTUP010000221 DAJTUP010000000
VERSION     DAJTUP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.225"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..556)
                     /locus_tag="OO468_004051"
     CDS             complement(<1..556)
                     /locus_tag="OO468_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR6260935.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAE"
     gene            complement(635..940)
                     /gene="elaB"
                     /locus_tag="OO468_004052"
     CDS             complement(635..940)
                     /gene="elaB"
                     /locus_tag="OO468_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR6260936.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(995..1456)
                     /locus_tag="OO468_004053"
     CDS             complement(995..1456)
                     /locus_tag="OO468_004053"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6260937.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            1521..2438
                     /gene="rbn"
                     /locus_tag="OO468_004054"
     CDS             1521..2438
                     /gene="rbn"
                     /locus_tag="OO468_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR6260938.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            2626..>2813
                     /locus_tag="OO468_004055"
     CDS             2626..>2813
                     /locus_tag="OO468_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR6260939.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGK"
BASE COUNT          726 a          714 c          648 g          725 t
ORIGIN      
        1 cttcggcgat ggttttcgtt gccagttcga ttaattgcgt ccagcccgtt ttgtccggcc
       61 agtgttgttc tcgcgtggtg gttaaatgta acccaggcaa gggcttgata ctcaccagtg
      121 tggcgataaa ttcttttgcc tgaatcgcat cgtgctgaag ggagctttcg ctgaatagcg
      181 tcagccgcag cgtggcttta ccgccacagc gtcgccattc caggcggggt aaaagtaaat
      241 tgccctgcga cgggtcaaaa gcattcagcc cccaaatgcg taagtcagcg tgttccgggt
      301 gctggcgaag aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc gcgcccagga
      361 cggcagcttc ttcatcacca ttacgttgtt gccagtagaa ttgcgggtac gtttgctgac
      421 tggccagcca gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata tcgataaccc
      481 gaatgccggg tgtcgccgga atctcttgcg acaaatggcg cagtagattt tccagcgccg
      541 tagtaagtga ttgcacgcgg acctctccct gttaaaagcc tcacattata cggggtacta
      601 caaaaaaaat gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc aacagtccta
      661 gtaccagccc gacggccgcg cccacaccaa ttccttgcca gggtttttcg tggacgtagt
      721 catcagcacg ataaacagcc tgcttcgccc gatagtaata actgtctgaa gcctggctaa
      781 cccgtttttt cacatcatcc agcgcttttt ctgcatgcgc tttcagctca acatatttct
      841 gatcggcggg atcgccagag gagcggagca cctcttccag tgtttcacta agcagcgtca
      901 ggtcgtcatc gatacgtgta tcaccaaact gattagacat tctcgttctc catttgcgta
      961 aaacctgtca ctaactatag acaagagttg ttgattacgc ctgaattacc tcgcgtgcca
     1021 tcccaatgtg tgggatacca tcttcttcat agacctccgt cacagggata aaaccgaaac
     1081 tctggtagaa gttttgcaga tgcgcctgcg ccccgaggta aacaggctta tcaggccagt
     1141 gatgcgtaca tgtttcgagt gttttactca tcagctgctg ccccactttt tcaccacgca
     1201 acgcttcact gacaatcacc cgacctataa cgaccggctc aagatcatca tcacttttca
     1261 gaatcctcgc atacgccacc agttcatcat ttttccaacc gagaatatgg cgattatccc
     1321 ccgtcaaatc atcaccatcg atatcctgat aagggcaatt ctgctcgaca acaaacaccg
     1381 cgcaacgcag ttgtaataag gcataaagct gggaaaccga taattcagag tgatgcagat
     1441 cttgccattc aatcataatg ctctcctttt agatgtatgc cttatactag ccggaatatt
     1501 ttttgaaacg tgatgaactc atggaattaa tttttttagg tacttcagcc ggtgtgccaa
     1561 cccgcacgcg caatgtcacg gcaatattgc ttaacctgca acatccgacc cagagcggac
     1621 tttggttgtt tgactgcggt gaaggcaccc agcatcagct actgcatacc gcctttaacc
     1681 ctggaaaact ggacaagatt tttatcagtc accttcatgg cgatcatctt tttggtttac
     1741 ccggcttgct gtgcagtcgt tctatgtcag gcattatcca acccttaacg atttatggtc
     1801 cgcaaggtat ccgtgaattt gtggaaaccg cgctgcggat tagcggctca tggaccgatt
     1861 atccgctgga aattgtcgaa attggcgctg gcgaaattct cgatgatggc ctgcgcaaag
     1921 taaccgctta tccgctggaa cacccactgg aatgttatgg ctatcgtatt gaagaacatg
     1981 ataaaccggg tgcattaaat gcccaggcat taaaagctgc tggcgtgccg cctggcccac
     2041 tgtttcagga attaaaagcg ggcaaaacaa tcacgctgga agatggaagg cagattaacg
     2101 gcgcagatta cttagctgct ccagtgccag gtaaagcgct cgctattttc ggcgataccg
     2161 gcccctgcga tgccgcactt gacctggcta aaggtgtcga tgtcatggtg cacgaagcga
     2221 cgctggatat aaccatggaa gccaaagcca atagtcgcgg ccatagctct acacgccagg
     2281 ctgcgacact agcccgtgag gctggagtcg gcaagctaat cattacccac gtcagctcgc
     2341 gctatgatga caaaggttgt caacacctgt tacgtgaatg caggtcaatt ttcccggcga
     2401 ctgaactggc gaatgatttc accgtgttta acgtttaact ctgcttatta ttaaaaacag
     2461 ggtgaaactt gccctgttat agcaacccgc gctacatata tccacccaca caggcatatt
     2521 gagcgatttt tgatatttat tcatctcctt acgcactata ttcagagcaa taagttgcct
     2581 cttttcatct tgattcagca caacctttgc aactaaaagg agcccatgat ggttacagtt
     2641 gtcagcaatt attgtcaatt atctcaaacc caactcagtc agacatttgc agaaaaattt
     2701 actgtgaccg aggaattact gcagtcttta aaaaaaacag cgttatccgg agatgaagaa
     2761 agtattgagt tactgcataa tattgcgtta ggttatgatg aatttggaaa aaa
//
LOCUS       DAJTUP010000222         2649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTUP010000222 DAJTUP010000000
VERSION     DAJTUP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.226"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(49..837)
                     /gene="phoH"
                     /locus_tag="OO468_004056"
     CDS             complement(49..837)
                     /gene="phoH"
                     /locus_tag="OO468_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR6260940.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1444..>2649)
                     /gene="efeB"
                     /locus_tag="OO468_004057"
     CDS             complement(1444..>2649)
                     /gene="efeB"
                     /locus_tag="OO468_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR6260941.1"
                     /translation="IGALAGSCPVAHAQKTQSAPGTLSPDARNEKQPFYGEHQAGILT
                     PQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQGGAAPETPNPRLPPLDSGILG
                     GYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTRFPNDSLDAALCHGDVLLQIC
                     ANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAARSKGKETPINLLGFKDGTANP
                     DSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFRVEFWDRTPLKEQQTIFGRDK
                     QTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPRTAESESSLMLRRGYSYSLGV
                     TNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEYVKPIGGGYFFSLPGVKDAND
                     YLGRALLQV"
BASE COUNT          627 a          747 c          639 g          636 t
ORIGIN      
        1 cacccaaaac aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc
       61 atgcagcgta cgttggcaaa gtgccgaacg tacgcaatcc tcttttccga accggacgat
      121 cccaaccatt tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc
      181 gcgaggcaaa tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag
      241 gcgggttaaa aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac
      301 aactgcattt tcaaaggtac gtccacgcat ataggcgaac ggcgcggttt ccaccttccc
      361 tatttccggt cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcaagtc
      421 gtagaccggg cgaaaatagg gagcaaactt ttctgcgata tcaccaggta agaagccaag
      481 atcttcatcg gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat
      541 cagggcctct gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt
      601 ggcgaatatc agctgcttac tctcaatagc ctgcagatag tgcaattgcg cttcatttcg
      661 cgcaaggatg ggcgaggtat cgcgactgtc gcgggccata ccaatggctt ctacgccgct
      721 catctgcaca agcgaggtga ccgattcttc ttcacgctgc ttatggctgc gcgaatcccg
      781 tctcagcaca cgttttgcct cgcgacgagc tttgatcact gctttttgtc ttcccatgga
      841 gagcaccttg agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg
      901 aacatcagag ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa
      961 acggctacgc acaccgtttg cggcgtcggt aacacgagta aagaaaggaa gtgagaaaaa
     1021 tacagagtga cgaagttgct accggaggag aaatctccgc gagtggtgga gcgttgctta
     1081 acaaaacatt gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca
     1141 ctgccgggaa cttccgctgc tacttaataa gtacaacaga actcgcattc attgcaacaa
     1201 tatattcact tatatttaac gataacaccc atttcggtga cattaatttc tactggaaag
     1261 atgacagagt gatgacagtg atggaaaaag ctgtgtgctt tccgcaggat tcgcagggct
     1321 ggtaaaatgt gcctcgtgtt tgccggattc ggcgcgaacg ccttacccga cctacaagtg
     1381 attaataatt tgtgcacaaa actcatgccc gataaacctg cgcatcgggc atatttgcgt
     1441 cgttcaaacc tgcaatagcg cccttcccag ataatcgttc gcgtccttca ctcccggcag
     1501 cgaaaaaaaa taaccgccgc cgataggttt aacgtattcc tccagcgctt cgccattgag
     1561 ccttttttgt actgtcagga agcctttttc cagatcgtgt tggtagcaga caaacagcaa
     1621 ccccatatcc agttgtccgg agttggtgac ccccaacgaa taactgtagc cgcgacgcag
     1681 catcaggctg gactcactct ccgccgtgcg gggattcgcc agccggatat ggctgtccag
     1741 cgcgatcacc ttcccttccg ggtcgctggc gtaatcaggc acatcatgct catgctgcat
     1801 tcccagcggc gcaccggttt gcttatcacg gccaaaaatc gtctgctgtt ctttcagcgg
     1861 cgttctgtcc caaaattcca ctcgaaactg aatcaagcgt actgcctgat agctgccacc
     1921 gattgtccac gcaggctcct gctgatctgc cgttacccac accacttttt gcatcaactt
     1981 atcattctgg ctatcgggat tggcagtgcc gtctttgaaa cccagcaaat taatcggcgt
     2041 ctctttgcct ttactacgcg ccgcgtgatc ggaaataaac ccttcccgct tccagcgcac
     2101 actgagcaaa tccggcgtgt gtttgatgat atcgcgcagc gcatggttca ccgtgtcctg
     2161 ggtgttggcg caaatctgta gcaacacatc accatgacat aacgccgcat ccagcgagtc
     2221 gttggggaaa cgcgtcatct tctgcagctt ttttggcatc tgtggcgcaa ggccaaagcg
     2281 ctcatcaaac aatgagtgac ccaccgataa cgtgatggtg agattatcgg gcgcaatgta
     2341 gccgccaaga atgccggaat cgagtggtgg caggcgcgga tttggcgttt ctggtgctgc
     2401 tccgccctga ctcagaaaag caaaacgctg agtcaacaag cgaaacaacc gctcaagatc
     2461 ggctttatcg ctggcaagca catcaaacgc caccagcatc attgcggcct gttgtggcgt
     2521 caggatccct gcctgatgct caccataaaa cggctgtttc tcattgcgtg catccggtga
     2581 aagcgtgccc ggtgcacttt gcgttttttg tgcatgagcg accggacaac ttcccgccag
     2641 cgcaccaat
//
LOCUS       DAJTUP010000223         2442 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTUP010000223 DAJTUP010000000
VERSION     DAJTUP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.227"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..568
                     /locus_tag="OO468_004058"
     CDS             <1..568
                     /locus_tag="OO468_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR6260942.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            526..1203
                     /gene="bglJ"
                     /locus_tag="OO468_004059"
     CDS             526..1203
                     /gene="bglJ"
                     /locus_tag="OO468_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR6260943.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="OO468_004060"
     CDS             complement(1241..2029)
                     /gene="fhuF"
                     /locus_tag="OO468_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR6260944.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2170..2406
                     /locus_tag="OO468_004061"
     CDS             2170..2406
                     /locus_tag="OO468_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR6260945.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          629 a          534 c          627 g          652 t
ORIGIN      
        1 attagtcatt gctgatttag ccggtgaaag tgaagatcca cgttctgttt gtgaacatta
       61 ttattcttta atctcacaat atcgggaaat tcactgggtt ttcatggtgt cacgctcctg
      121 gtattcccag gctgtagaac tgctcatgtg ccctacggcg acgttattgt ctgatgttga
      181 acccattgag aatctggtca agaccgtacg ttccggcaat acgcacgcag agcgtatcag
      241 cgccatgctg acctctccgg caatgactga aactcatgat tttagctatc gctccgtcat
      301 tctcactctt tcagagcgca aggtactgcg gctattaggt aaaggatggg gcatcaacca
      361 gatagcttca ttgcttaaga aaagtaataa aactatcagc gcccaaaaaa acagtgcgat
      421 gcgtcgactg gcaattcaca gcaacgctga aatgtatgca tggataaata gcgcgcaggg
      481 tgcaagagaa cttaacttgc cttctgttta tggagatgcc gcagaatgga acacagccga
      541 attaagaaga gaaatgtcgc actcatagaa aaatgcgtca tgagtagtat cggtattgag
      601 agtttattca gaaagtttgc gggcaaccct tataagctcc atacctatac cagtcaggag
      661 tcatttcagg atgccatgtc gcggatctcg tttgcggcgg tcattttttc tttttctgcc
      721 atgagaagtg agcgcagaga gggattatct tgcctgactg agctggcgat taagtttccg
      781 cgtacccggc gtttagttat tgtggatgat gatattgaag ctcggctgat tggttcattg
      841 tcgccatcac cgctggacgg tgtattaagt aaagcgtcaa cgctggagat ttttcatcag
      901 gaactctttt tgtcattaaa tggtgtacgt caggcgaccg accgactgaa caatcagtgg
      961 tacattaacc aaagccggac gttaagcccg acggagagag aaatattgcg ctttatgtcg
     1021 cgtggctact caatgacaca aattgccgag cagcttaaac gtaatatcaa aacgatccga
     1081 gcgcataaat ttaatgtgat gtcgaaactg ggcgtcagtt ctgatgcagg gttgttggag
     1141 gccgaagata ttctgttatg tatgcggcat tgcgaagcaa gtaatgtgtt gcatccctat
     1201 taatccgcat gatgccgggt ttacttcccc ggcagtgctt tcatttcagc gtacaatagc
     1261 cacattgctg cacatccggt aagcgataac gctggcagca agtgcggcgc accagcaggc
     1321 cgtcgcgcag taccacggta cgccagagtg gattatcttc accgttcgtg agcgttttct
     1381 caaaaaagag ggcatggcgc agcgattcaa cagtagcctc gccgagcagt tgcttcatct
     1441 cagtgagata ccagttgatc aaataaccgg tattactcca gataagtttg ccgttgatct
     1501 ctccggtcgc ttctagtgct tgcacaaccg gaaccagcgc ctggctgatt aacgtttcca
     1561 ttcgctgctg cggcgaatgt ggtgttgcgt ttttatcttc acacacatcg acccagaaac
     1621 aggcgacgcg tccggtttcg tgaaactcag catggaaatg ttccggcgac acatctaatg
     1681 ctttttcctg cgtcagtagc gccagcatta atggtggcac catcaggccg atataccatt
     1741 gtgcccatag tgagatcagc ggtttgttct cgcggatcat cgttggttgg ttgcgataga
     1801 tatgatcgga atagaccgcc agtagagaac ttagcgcatt cggtgatgac cattgcgcca
     1861 gcgtcatggc gttaagcgga gcgggttcat ccaggcggat aaactccagc aaatgttcac
     1921 gatgtttggc gatcatcgcc cgtacggctt gtgcaagccc agcatcctgc ggctggagat
     1981 gcgttcgcca gatgacatct tcatagagcg gtgcggaacg ataggccata atcgtgatag
     2041 taatctaaat gataatgatt gctaatccta gcgataggtt tactcgatag caagggattt
     2101 ctctggcttg caaatgataa aaattatcat atgatattgg ttttcattat caatgaaaga
     2161 gatgaaacca tgttgcaacg tacgctgggc agtggctggg gagtgttgct gccgggattg
     2221 ctgattgcag ggctgatgta tgcggattta tcgccggatc agtggcggat tgtcattctg
     2281 atgggattag tattgacgcc ggtgatgctg tatcacaaac agttgcggca ttacattttg
     2341 ctaccatcgt gcctggcgct tattgctggc atcatgctga tgataatgaa tttgaatcag
     2401 ggatgacaaa acagggaaga aacaagaaag gaagtaaaga ta
//
LOCUS       DAJTUP010000224         2309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTUP010000224 DAJTUP010000000
VERSION     DAJTUP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.228"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..216
                     /locus_tag="OO468_004062"
     CDS             <1..216
                     /locus_tag="OO468_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HCR6260946.1"
                     /translation="VYSDFWESVAGKTCGDWYKLPTQVVRKPLSNIASKKRSEYRKRY
                     ALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            281..1225
                     /gene="lpxM"
                     /locus_tag="OO468_004063"
                     /gene_synonym="msbB"
     CDS             281..1225
                     /gene="lpxM"
                     /locus_tag="OO468_004063"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR6260947.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(1379..>2309)
                     /locus_tag="OO468_004064"
     CDS             complement(1379..>2309)
                     /locus_tag="OO468_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR6260948.1"
                     /translation="PQLHRGDRVSDVARTLCCARSSVGRWINWFTQSGVEGLKSLPAG
                     RARRWPFEHICTLLRELVKHSPGDFGYQRSRWSTELLAIKINEITGCQLNAGTVRRWL
                     PSVGIVWRRAAPTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPKIGAD
                     WQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKATYRR
                     AKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDTITRN
                     HQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          595 a          526 c          555 g          633 t
ORIGIN      
        1 gtctacagtg atttctggga gtctgtagca gggaaaacct gtggtgactg gtacaaatta
       61 ccaacacagg tggttcgaaa gccgctaagc aatatagcta gtaaaaaacg ctctgagtat
      121 cgaaaacgat acgctttgct ggattatatc catgaaactg ctatccgctc tttggatgca
      181 tatcctgtaa actcagaaca tcaggactta aattaaggtt tgatgtattc tctgaataaa
      241 atattaatga tgattttggt aggggcattc gcactaaata atgaaaaaat acaaatccga
      301 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggttta catggttcgt
      361 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      421 caaaataggg cgttgggtgg gcagattgag cagaaaagct cgtcgcaggg cgacgattaa
      481 tttatcgctt tgtttcccgg aaaagagtga tacagaacgg gaaataattg tcgacaatat
      541 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
      601 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcggcacaa
      661 taacagaaat gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt
      721 gctggcagct cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat
      781 tgattatgtc tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga
      841 tgggataaaa ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga
      901 tcaggatcat ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac
      961 attaccaatt attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc
     1021 ggtttatgat gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg
     1081 cattgccagc gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt
     1141 ggtggggtca catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc
     1201 aaacgaagcg gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc
     1261 ttacaggtta ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga
     1321 ctgtgtagat gcagttcgtt aaatttaatt tatgcctaaa tagctgcgct taataccgct
     1381 acacttttgc cagcccatgt ttgcctccgg ggaatgggct gacggtttcc ataaaatggc
     1441 gaactttttt caacagttgc cacattgagc ggcactgatg attacgcgtt attgtgtcgt
     1501 gaagtgcctg ccatagccgt tcaacatgat tcacccatgg cgagtaaacc ggctgataaa
     1561 tgcccctgaa cttcgggttc tccttcagcc agctctgtgt ttcccggctt ttgtggataa
     1621 tgtagttgtc cacgatcagc gtgatggttt tcgcacgacg gtatgtcgct ttaagccgct
     1681 tcagcaggct gatgaacagc gccgaacttt tgctgttgcc gcccacatag ctgactttac
     1741 ctgtcccgct gtgcagcgct ccggccagat aatatttttc attctgtccc ggcgtgacca
     1801 cccgtttttg ctgtccgcgc agttgccagt ccgcaccgat tttgggatga agatggatat
     1861 ccacttcatc ttcataaaag accggatgct ctgcgctgca ttcgtccagt gctttatgga
     1921 ttgctgccat cttttcatct ttatgcgggt cacggatacg cagagttggc gcagcccttc
     1981 gccacacaat ccccacagac ggcaaccagc ggcgaacggt tccggcattt aactggcaac
     2041 cggttatctc attgattttt attgccagca gttctgtact ccagcgtgaa cgctggtagc
     2101 caaagtcgcc gggagaatgt tttaccagct cacgtaacag tgtgcagata tgctcaaacg
     2161 gccagcgacg ggcacgcccg gcaggtaatg atttcagtcc ctcaacaccc gactgcgtga
     2221 accagttaat ccagcgtcca acagaggagc gggcgcagca gagcgttctg gcaacgtcgc
     2281 tgacacggtc gccccggtgc agctgaggt
//
LOCUS       DAJTUP010000225         2300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTUP010000225 DAJTUP010000000
VERSION     DAJTUP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.229"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..1843
                     /gene="kup"
                     /locus_tag="OO468_004065"
     CDS             <1..1843
                     /gene="kup"
                     /locus_tag="OO468_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR6260949.1"
                     /translation="PAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGVERDAVFGFL
                     SLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSMLVIMGLIGGS
                     FFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKHGTAMVGKLF
                     APIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITG
                     GEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWA
                     LIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVN
                     WMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALIL
                     IAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLE
                     AMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPY
                     VHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHES
                     LILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2010..>2300
                     /gene="rbsD"
                     /locus_tag="OO468_004066"
     CDS             2010..>2300
                     /gene="rbsD"
                     /locus_tag="OO468_004066"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR6260950.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGN"
BASE COUNT          487 a          555 c          612 g          646 t
ORIGIN      
        1 gcccgcgatt accctcgcgg cgattggagt tgtctacggc gatattggta ccagcccgtt
       61 atatacactt cgtgaatgtt tgtccggcca gtttggtttt ggcgttgaac gcgatgccgt
      121 gtttggcttt ttatcgctga tcttctggct gctaatcttt gtggtttcca ttaaatatct
      181 caccttcgtg atgcgggcag ataacgccgg tgaggggggg atcctgacgt tgatgtcgct
      241 tgccgggcgt aatacgtcgg cgcgaaccac atcaatgctg gtgattatgg ggctaatcgg
      301 cggcagcttt ttctatggtg aagtcgtcat aacacccgct atttcggtga tgtcagccat
      361 tgaaggtctg gaaatcgtcg ccccgcagct ggatacctgg atagttcccc tctcaattat
      421 cgttctcact ttattattta tgattcaaaa gcatggcacg gctatggtcg gtaagctgtt
      481 tgcgccgatc atgctgacct ggtttttgat tctggcagga ctggggttac gtagcattat
      541 tgctaacccg gaagtgctgc atgcgctgaa tccgatgtgg gcggtgcatt tcttccttga
      601 atacaaaacg gtttctttta ttgcattagg ggcagtggtg ctgtcgatta cggggggcga
      661 ggtgctgtat gctgatatgg ggcactttgg taagttctct attcgcctgg cgtggttcac
      721 cgtcgtatta ccttccttga cccttaatta cttcggtcag ggagcgttgt tgttaaagaa
      781 cccggaagcg attaagaacc cgttcttcct gttggcaccg gactgggcgc tgatcccact
      841 gctgatcatc gccgcactgg cgacggtaat tgcctcgcag gcggttatct ctggcgtctt
      901 ctcattgacg cgtcaggcgg tacgtctggg atatttgtcg ccgatgcgca ttattcacac
      961 ctccgaaatg gagtcagggc aaatctatat tccgtttgtg aactggatgc tctatgtcgc
     1021 ggtcgtgatt gtgattgtca gctttgagca ctccagcaac ctggcggcgg cgtacgggat
     1081 tgcggtgacc ggaaccatgg tactgacgtc tattctctcg actaccgtgg cacgtcagaa
     1141 ctggcactgg aataagtatt ttgttgcgct gatcctgatt gctttccttt gtgtcgatat
     1201 tccattgttc accgctaacc tcgataaact gctctccggc ggctggttgc cattgagcct
     1261 cggtactgtg atgtttatcg tgatgaccac ctggaagagc gagcgtttcc gcttgctgcg
     1321 gcggatgcat gaacatggta actctctgga agcgatgatt gcttcgctgg agaaatcacc
     1381 gcctgttcgc gtgcccggga ccgcggtgta tatgtcgcgt gcaatcaacg tcattccctt
     1441 tgcgctgatg cataacctta aacataacaa ggtattgcat gagcgggtga ttctgttaac
     1501 tctgcgcacc gaagacgcgc catatgtcca taacgtccgt cgggtacaga ttgaacaact
     1561 gtcgcccact ttctggcgcg tggtggcaag ttatggttgg cgagaaacgc caaacgtgga
     1621 agaagttttc caccgctgcg gtctggaagg attaagttgc cggatgatgg aaacctcctt
     1681 ctttatgtcg catgagtcgt tgatcctcgg caaacgcccg tggtatttgc gtctgcgcgg
     1741 caagctgtac ttgctgctgc aacgtaatgc gctgcgtgca ccagatcaat ttgaaatccc
     1801 gccaaacagg gttatcgaac tgggtactca ggtcgaaatc taacgccaga cgcctccttt
     1861 cttcataagg gggcgttttt gttttcatgg ttaatcacca tgtaaaacgt ttcgaggttg
     1921 atcacatttc cgtaacgtca cgatggtttt cccaactcag tcaggattaa gctgcggatc
     1981 agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt aattctgata
     2041 tttcatcggt gatctcccgt ctgggacata ccgatacgct ggtggtgtgt gatgctggtt
     2101 tacccatccc caaaagtaca acgcgtatcg atatggcatt aacccaaggt gtaccttctt
     2161 ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt cgaggctgcc attatcgcgg
     2221 aagagatcaa acaacataat ccgcaactcc acgaaacgtt gctcactcac cttgagcagc
     2281 tgcaaaaaca ccagggaaat
//
LOCUS       DAJTUP010000226         2250 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTUP010000226 DAJTUP010000000
VERSION     DAJTUP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.230"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO468_004067"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO468_004067"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR6260951.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          553 c          666 g          457 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac
//
LOCUS       DAJTUP010000227         2248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTUP010000227 DAJTUP010000000
VERSION     DAJTUP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.231"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..662
                     /locus_tag="OO468_004068"
     CDS             <1..662
                     /locus_tag="OO468_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCR6260952.1"
                     /translation="TPVWQRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVIL
                     LISIYGLLKALRRDLTKRESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMD
                     DVIQPAMTLVQEELNKQGTISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFA
                     LAAMDNDEQQTEQHRYYRAEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLV
                     R"
     gene            725..2002
                     /locus_tag="OO468_004069"
     CDS             725..2002
                     /locus_tag="OO468_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR6260953.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          606 a          496 c          550 g          596 t
ORIGIN      
        1 acacacccgt ctggcagcga atattctggg cctcgctcat gggcattgtt gcaattgcgc
       61 ttctccttgc cggagggcta agtgcgctgc aaacggtgac aatagcgagt gcattgccct
      121 tctcagtgat cttactaata tctatatacg gacttttaaa agctttgcgc cgggatttga
      181 ccaagcgtga aagcctgagc atggcgacaa ttgctcctac ggctgcacgt aacccaattc
      241 cttggcagag aaggttacgc aatatcgcgt atctgccgaa gcgatctctt gtgaaacgtt
      301 ttatggacga cgttatccag cctgccatga cgctggttca ggaggaactg aacaagcagg
      361 ggacgataag ccacattagt gatgcagtcg acgatcgtat tcgtcttgaa gtcgatttgg
      421 gcaacgagct gaattttata tatgaagtga ggcttcgcgg gtatatctca ccgaccttcg
      481 cgctcgccgc aatggataat gatgagcagc agactgaaca acatcgatat tatcgcgctg
      541 aggtttatct caaagaaggc ggtcaaaatt atgatgtgat gggctggaac caggaacagc
      601 tgattaatga catactggac cagtacgaaa aacacctgca cttcctgcac ctggttcgtt
      661 aatagcaaca tgccgtcctg ggggcggcaa ttattatccc ggccgcaata tgagggaatg
      721 cagaatgatt tcacgctgga aatggatgct gaagcagaca attaaaaaac tatggttcag
      781 ggcaacgtta ttcgcaattg tcgcgataat aacggccctt ttatcaattc tttttaaatc
      841 aatgatacct gagtcggttt ccgtgaaggt tggtgcggaa gcagtcgata acattctgaa
      901 catactggca tcgagtatgc tggcagtgac cacattttcg ctgagtatca tggtcacagc
      961 ctacggttca gccactacta atgtgactcc cagagctacg cgtttagttg ttgaagacgt
     1021 caccacacaa aatgtactgg ccaccttcat cggttctttt ctcttcagtc tggtagggat
     1081 tattgccctc aatatgggag cttatggaga aagggggaga gtcattttat tcattgtcac
     1141 actggttgtc attgccttaa tcctcatcac attgcttcgc tggatacagc atttgacctc
     1201 tctggggagg gttggtgaga caacggcaca agtagaacag gcggccatcg aaacatttat
     1261 tgcgagagca agaaatccct gtctcggtgg atatccatgg cttgagaaca atgaacagcc
     1321 gaaaggaacg gttgcagttt atccgaagaa gattggctat gttgaatata ttgatatggt
     1381 gaaacttagc aagctgctga ccaatgatcc ccgtcatgta tacctcgtgg cgcagccagg
     1441 cagtttcata catccgtcca tgccagtttt gtacctgagt cagggccagg agtcgtcaat
     1501 caacaccgat ttacttgaga cgattattgt ctcggatgca cgttcatttg ctcaggatcc
     1561 tcgattttgt cttagcgtca tggccgaaat agcctgccgg gccctttccc ctgcagtgaa
     1621 cgatcctgga accgccattg atgtcattgg cagaggtgtt cgtatacttt ccacttacgc
     1681 gcagaataaa tctgatgaaa tagaagtgaa atatccttca gtacatgttg caccacttca
     1741 gaataacgat ctactggaag actttttctc acctgtcgcg cgcgatggtg ccagtatgag
     1801 ggagattcag ataagagtcc ttaaaggact gtcgatgctg agtaaaggtt ggcctgagat
     1861 atttgctgaa gccgcacata ccctggcatt tgaaacatta gagcatgcaa ctcgtgctga
     1921 ccatatagat tctgatagat atctaataaa atcaacttat tataatttat ttagtggcga
     1981 atattctaat aaaaaaacat agctgcagag caagcaattc ggttgttggc aagtcaaggg
     2041 caaagcattc gcgtgagaat gggcgtatgc tttgtgccaa aagcaaacgt tgctaattct
     2101 gcactgttca gcgatggcgc agtagagaca ggcactgcca ctgtccagtc ggcaaaagcg
     2161 aacactttgc cactgcgggg ataaactgat tgaagctctc gcgcaaaatc acttattctt
     2221 ttgcgttaat aaaatgtagg agatgggt
//
LOCUS       DAJTUP010000228         2248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTUP010000228 DAJTUP010000000
VERSION     DAJTUP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.232"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            144..287
                     /locus_tag="OO468_004070"
                     /pseudo
     CDS             144..287
                     /locus_tag="OO468_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            434..700
                     /gene="ospE1"
                     /locus_tag="OO468_004071"
     CDS             434..700
                     /gene="ospE1"
                     /locus_tag="OO468_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR6260954.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1090..>2248
                     /locus_tag="OO468_004072"
     CDS             1090..>2248
                     /locus_tag="OO468_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6260955.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSTPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISAPEHSLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTS
                     GFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRKTLLVH"
BASE COUNT          730 a          508 c          395 g          615 t
ORIGIN      
        1 catcccgtat tgggcagtat gatgccggaa agctgcgagg ctccacacct gccagtttca
       61 cccttttaac catcatacta ctccgtgttt aattgtgtgg gatgcgttca ttacatttta
      121 acagtgtcac cccgtcggcc acgaaagata aacaggtgac cagaaaaggg atcttcatcc
      181 agcacatgct atatcttgaa cgtaacaacc tgaccctccg cacccgcatc aagcggctgg
      241 cacgtaaaac aatctgcttc tcgcgttccg tggaaatcca cgaaaagtca tcggctcctt
      301 cattgaaaaa cacatattct actgattgga agcgccaccc gaaaaagtat cgttttttta
      361 cagtaaactt catttagccg actataatgt aaaaaaataa attttttacc aaaatataac
      421 ataaggaagt aatatgctta cacaaactat attcccttgt cttccacaaa aacaagaaaa
      481 tatcatcctt gaagtatcaa atcctgtatt attatcttct actgttacta cagatggata
      541 tactgttttc aataaaaaag cagctatata cgaattacaa atacctgctg ccagccggac
      601 gaaaacgttg aaattcactg ctacagaaat gcaatggttg acaaagataa atgaagccgg
      661 aattgatgaa aaacaaagcc aacggtattc tgatttctga agttaatttc agactcgttt
      721 aagctccatt ttcaaaaaaa atatatcagg gtttgttact aataacttct gaaaacagaa
      781 aaaaatagag cagtaattct atttcagaga ggagtaactc catctatcct cgatacgtac
      841 tgaacgttat cgtgatatat tcatcatgct ttcttcacca ttattcgagt atagggagag
      901 acgcaatgac cttaaatacc tttttatggc tgaattacca ttctcaggaa taacagcaga
      961 ctccttccca tagacggctt agggggaaag caatacccgg agaaagggta caaagcaaac
     1021 tgttttaaaa ttaataagcc atattctatt taactagaat tcttatcgtt gataagaatt
     1081 ctagttataa tgattaaatc aaccaatata caggcaatcg gttctggcat tatgcatcaa
     1141 ataaacaatg tatactcgtt aactccatta tctttaccta tggaactgac tccatcttgt
     1201 aatgaatttt atttaaaaac ctggagcgaa tgggaaaaga acggtacccc aggcgagcaa
     1261 cgcaatatcg ccttcaatag gctgaaaata tgtttacaaa atcaagaggc agaattaaat
     1321 ttatctgagt tagatttaaa aacattacca gatttaccgc ctcagataac aacactggaa
     1381 ataagaaaaa acctattaac acatctccct gatttaccac caatgcttaa ggtaatacat
     1441 gctcaattta atcaactgga aagcttacct gccttacccg agacgttaga agagcttaat
     1501 gtgggtgata acaagataaa agaattacca tatcttcctg aaactctaac tcatttacgg
     1561 atccataata accgattgca tattctgcca ctattgccac cggagctaaa attactgata
     1621 gtttctggaa acagattaga cagcaccccc ccctttccag ataagcttga agggctggct
     1681 ctggctaata attttataga acaactaccg gaattacctt ttagtatgaa cagggctgtg
     1741 ctaatgaata ataatctgac aacacttccg gaaagtgtcc tgagattagc tcagaatgcc
     1801 ttcgtaaatg ttgcaggtaa tccattgtct ggccatacca tgcgtacact acaacaaata
     1861 accaccggac cagattattc tggtcctcaa atatttttct ctatgggaaa ttctgccaca
     1921 atttccgctc cagaacactc cctggctgat gccgtgacag catggttccc ggaaaacaaa
     1981 caatctgatg tatcacagat atggcatgct tttgaacatg aagagcatgc caacaccttt
     2041 tccgcgttcc ttgaccgcct ttccgatacc gtctctgcac gcaatacctc cggattccgt
     2101 gaacaggtcg ctgcatggct ggaaaaactc agtgcctctg cggagcttcg acagcagtct
     2161 ttcgctgttg ctgctgatgc cactgagagc tgtgaggacc gtgtcgcgct cacatggaac
     2221 aatctccgga aaaccctcct ggtccatc
//
LOCUS       DAJTUP010000229         2233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTUP010000229 DAJTUP010000000
VERSION     DAJTUP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.233"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(64..822)
                     /locus_tag="OO468_004073"
     CDS             complement(64..822)
                     /locus_tag="OO468_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR6260956.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO468_004074"
     CDS             complement(830..1933)
                     /locus_tag="OO468_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR6260957.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2233)
                     /locus_tag="OO468_004075"
     CDS             complement(1943..>2233)
                     /locus_tag="OO468_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR6260958.1"
                     /translation="LGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGY
                     PDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          611 a          600 c          512 g          510 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cag
//
LOCUS       DAJTUP010000230         2188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTUP010000230 DAJTUP010000000
VERSION     DAJTUP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.234"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..893
                     /locus_tag="OO468_004076"
     CDS             <1..893
                     /locus_tag="OO468_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6260959.1"
                     /translation="ELARVGVNPHDVAQMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQ
                     DKMQGRQVTMRGQDLDSQTAARNQAITMRGQDIQANLGQQRINLDAETNRINNENKRL
                     DRMLSAETNDLKRQEIQSRIAANNQQLQQKQQALNDGYKDGINTLTTSMFTLNDIVSS
                     PSLKSITGLRGVIPNVPGSQAADTQARLDTFKSQAYLTAVQAMRGMGALSDAEGKKLD
                     QAVGSLQNSQSEESFRRNAGVILNTLNQKRNEAVGKYVQQNGIKRVEAPQASIDYLKQ
                     HPELSIDFINRYGYLPSLGQ"
     gene            894..>2188
                     /locus_tag="OO468_004077"
     CDS             894..>2188
                     /locus_tag="OO468_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR6260960.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDEL"
BASE COUNT          602 a          559 c          574 g          453 t
ORIGIN      
        1 gtgagttagc tcgtgttggc gttaatcctc atgacgtcgc tcagatgtac caacagaacc
       61 cgcggcagtt cggcgaattt gtcgatcacc tggggatgaa cagtctcggg cccgaaaaat
      121 actttgacct acaggataaa atgcagggtc gccaggttac catgcgcggt caggatctgg
      181 attcgcaaac cgccgctcgg aatcaggcaa tcacaatgcg cggacaagat atccaggcga
      241 atttaggtca gcagcgcatt aatctggacg cagaaacaaa ccgcattaac aacgaaaata
      301 agcgccttga ccggatgcta tcagcagaaa ctaacgacct gaagcgccag gaaatacaga
      361 gccgcatagc agccaacaac cagcagttgc agcagaagca gcaagcgcta aatgatggct
      421 acaaagacgg catcaacacc ctcacaacca gcatgttcac tctgaacgat atcgttagtt
      481 ctccttcact taagagcatt acaggcttac gtggagtaat ccccaacgtt ccaggctcac
      541 aggctgcaga cactcaggca cgacttgata cctttaaatc acaagcatac ctgacagcgg
      601 ttcaggccat gcgaggcatg ggcgcacttt ctgatgccga gggcaaaaag ctcgaccagg
      661 ctgttggttc gctgcagaac tcgcagagcg aggagtcctt tcgtcgcaac gctggcgtca
      721 tcctgaacac gctcaaccag aagcgtaatg aggcggttgg taagtacgtt cagcaaaacg
      781 gtatcaagcg agtggaagcg cctcaggctt ctatagatta cctgaagcag caccccgagc
      841 tgtcaatcga cttcattaat cgctacggat atcttccatc tttggggcag taaatggcta
      901 attaccgtga tttgttagag caggctggcg cacgttacgg tgtgccagaa gggttgatga
      961 ctgcactggg tgccaaggag tcttcttaca accctgccgc agtaagctcc gccggggctg
     1021 taggattgac tcaggtcatg cctgggacat ggcgtgatat gggttatacc gatgagcaaa
     1081 tgcaaaaccc cgaatatcag gctgacgctg gcgcgcgcta tctggcaaag atgtaccagc
     1141 agtttggtaa ctggcgtgac tctcttcagg cttatcacga cggtcccggc aacgttatga
     1201 aggcaaagcg tggtgaatat acgccaggac ctgaaggccg cggttacgtt gatgatcgct
     1261 ttgctcaatg ggctggtgac ccggtgacag actcaacagt cgaacagcgc gccacctctg
     1321 caaaggtaca tcctcagcaa gaccctaaca acccgtttgc acaactggaa gtacagtcat
     1381 ccgaacaagt atcggcatca ggtgtgcagt cagacccaaa taatccattt gctcagattg
     1441 agcagcaggc agccagtcag cagccacctc aacccgtaag ttctgtcgca ccgaaacctg
     1501 ttcagcaaca aacagttaat caggccaata atgcaccaac acgggaagaa ccatcattga
     1561 tgcaacaagc tggcgattgg ctcactggtg gtcaaagtgc agggcaaatt gcagaacagg
     1621 ctggtcgtgg tctggtaaac ataccatttg acgtattgca gggtggcgca agtctgatta
     1681 atgcaatcag tcaggggctt ggggggccaa aagtattgga tgatgtttat cgtccagtag
     1741 acagaccgac agacccctac gcgcaagctg gagagtcaat aggcggttat cttgttcctg
     1801 gagcaggtgt agctggaaac atggtcattg gttctctcgc tgacgcggcg aatcaacggg
     1861 gtgattttgc cgaaaatgcc gctattaatg ccggacttag cattgctacg catggcctga
     1921 taaatggcgt tacccgtggt gttcgtggtg catcaaatat aattagtggc aataaaacat
     1981 ctgcacagag agcgaccact gcgccaacag aaacatcacc attctccggt gatgccgctg
     2041 cagcaacaaa tcctgcggtt catgccgcag aggcaagagt agcacaaggt gtaccaatga
     2101 cgcctgcgac gaagaaccca gaggaagtcg ttcgcacagt agcagcacaa aaaaggccaa
     2161 atctcgcttc atcgcttgat gaactaga
//
LOCUS       DAJTUP010000231         2184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTUP010000231 DAJTUP010000000
VERSION     DAJTUP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.235"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            89..808
                     /locus_tag="OO468_004078"
     CDS             89..808
                     /locus_tag="OO468_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR6260961.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            819..>2184
                     /locus_tag="OO468_004079"
     CDS             819..>2184
                     /locus_tag="OO468_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR6260962.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEA"
BASE COUNT          557 a          473 c          623 g          531 t
ORIGIN      
        1 aaaagaacgc catatttatt gatgattgat cccccggtgg gggggaaaca tcgggcataa
       61 atgggcatga agtaatggag tattagttgt gaatgtcaat ttctttgtca cctgtattgg
      121 tgacgccctg aaatcaagaa tggcacgaga ctccgtgctg ctactggaaa aactcggctg
      181 tcgcgtaaat ttcccggaga aacagggatg ctgcggtcag cctgcgatca atagcggtta
      241 tatcaaagaa gcgattccag ggatgaaaaa tctgatcgcc gcactggagg ataacgacga
      301 tcccattatt tcaccggctg gctcttgcac ctatgccata aaaagttacc cgacgtatct
      361 ggcggatgaa cctgaatggg catcacgtgc cgaaaaggtt gccgcgcgta tgcaggatct
      421 cacctctttt attgttaata aattaggggt agtcgatgta ggtgccagtt tgcaagggag
      481 agctgtgtat cacccatctt gtagcctggc ccgtaagctg ggagtgaagg acgagccact
      541 tacgctgctg aaaaatgtgc gtggactgga gctgttgacc tttgctgaac aggatacctg
      601 ctgcggattt ggcggcacgt tctcggtcaa aatggccgaa atatccggcg agatggtgaa
      661 agaaaaggtt gtgcacctga tggaagtccg ccctgagtat ttaattggtg ctgacgtgag
      721 ttgcctgctg aacatcagtg ggcgattaca acgggaaggg cagaaagtca aagtgatgca
      781 tattgctgaa gtgttgatga gccgctgagg atataaagat gtcgatcaaa accagtaata
      841 cagattttaa gacacgcatc cgtcagcaaa ttgaagatcc gatcatgcgc aaagcggtgg
      901 caagcgcgca gcagcgtatt ggggcaaatc ggcaaaaaat ggtcgatgaa ttggggcact
      961 gggaggagtg gcgcgatcgg gccgcccaga tacgtgatca tgttctgagt aatctcgacg
     1021 cttatctgta ccagctctca gaaaaagtga cgcaaaacgg cggtcacgtc tattttgcaa
     1081 aaaccaaaga agacgctacc cgctacattt tacaggttgc ccaacgcaaa aatgcccgga
     1141 aggtggtgaa atctaaatcg atggtgaccg aagagattgg tgtcaatcat gtgttgcagg
     1201 atgctggcat tcaggtgatt gaaaccgatc tgggtgaata tattctccag ctggatcaag
     1261 atccgccatc tcatgttgtg gtcccggcaa ttcataaaga tcgccatcag atccgtcgag
     1321 tgctacacga acgtctgggc tatgaggggc cggaaacgcc tgaagcgatg accttattca
     1381 tccggcaaaa aatccgtgaa gatttcctca gtgctgaaat aggtattacc ggctgtaatt
     1441 tcgcggtggc agagaccggt tcggtatgcc tggtgaccaa tgaaggtaat gcgcgaatgt
     1501 gtaccacgct gcctaaaacg catattgcag tgatgggaat ggagcgtatt gcccccacgt
     1561 ttgccgaggt agatgtattg atcaccatgc tggcgcgcag tgccgttggt gcacgtttga
     1621 cgggatacaa cacctggctg acagggccgc gcgaagcggg gcacgttgat ggtcctgaag
     1681 agtttcatct ggttattgtc gataacgggc gttctgaggt gctggcctct gaatttcggg
     1741 atgtgctgcg ctgtattcgc tgcggggctt gtatgaatac ttgtccggca tatcgccata
     1801 ttggcggtca tggatatggc tctatttatc cagggccaat tggtgcggtg atttctccgc
     1861 tacttggcgg ctataaagat tttaaagatt taccctacgc ctgctcttta tgcactgctt
     1921 gtgacagcgt gtgtccggtg cgtattccgc tgtcaaaact gattttgcgt catcgtcggg
     1981 tgatggctga aaaagggatc accgcaaaag cagagcaacg ggcgataaaa atgttcgctt
     2041 atgccaatag tcatccagga ttgtggaaag tcgggatgat ggccggcgct catgcggcaa
     2101 gctggtttat caatggcggc aaaacaccac tcaaatttgg cgcgattagc gactggatgg
     2161 aagcacgcga tcttcctgaa gctg
//
LOCUS       DAJTUP010000232         2035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTUP010000232 DAJTUP010000000
VERSION     DAJTUP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.236"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            6..224
                     /locus_tag="OO468_004080"
     CDS             6..224
                     /locus_tag="OO468_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR6260963.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO468_004081"
     CDS             226..591
                     /locus_tag="OO468_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR6260964.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO468_004082"
     CDS             588..1253
                     /locus_tag="OO468_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260965.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO468_004083"
     CDS             1253..1618
                     /locus_tag="OO468_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR6260966.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          556 a          419 c          541 g          519 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggtt
//
LOCUS       DAJTUP010000233         2034 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTUP010000233 DAJTUP010000000
VERSION     DAJTUP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.237"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..642
                     /locus_tag="OO468_004084"
     CDS             <1..642
                     /locus_tag="OO468_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR6260967.1"
                     /translation="GQALAGGIVLQRTRVIYDASRKEAALPVANKGAETPYLLQSWVD
                     NIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENKESLFYINVRAIPAKKKSD
                     DVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRSDHSLNIYNPTEYYVVFAG
                     LAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTETRPLQ"
     gene            671..>2034
                     /locus_tag="OO468_004085"
     CDS             671..>2034
                     /locus_tag="OO468_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6260968.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRT"
BASE COUNT          598 a          485 c          461 g          490 t
ORIGIN      
        1 ggccaggcgc ttgcaggggg tatcgtttta cagcgaacgc gagtgatcta tgatgccagc
       61 cgcaaagagg ctgcgttacc tgtcgcaaac aaaggcgcag aaacgcctta tttactgcaa
      121 tcatgggtag ataatataga tggtaaaagc cgtgccccat ttattataac cccaccgcta
      181 tttcgtcttg aggctagcga tgactcatca ctgcgaatta ttaaaacagc tgataacctg
      241 cctgaaaata aagagtcgct gttctacatt aatgttcgtg ccattccagc aaagaaaaaa
      301 tcagatgatg ttaatgctaa cgagttgacg ctggtattta aaacgcgggt caaaatgttt
      361 tatcgccccg cacacctgaa gggacgggta aacgatgcgt ggaaatcact ggaatttaaa
      421 cgtagtgacc attcactcaa tatatataac ccaactgaat attacgtcgt atttgccgga
      481 ctggcagtcg ataaaaccga tctcacaagc aaaattgaat atatcgcgcc cggagaacat
      541 aaacagttac cacttccagc atctggcgga aagaacgtga aatgggctgc gatcaacgat
      601 tatggcggca gttccgggac agaaactcgt ccactgcaat aaaaaatata aaaacacagg
      661 ccatcaggga atgccacaac gacaccacca gggacataaa cgcacaccga aacagttggc
      721 gctcattatc aaacgttgtt tgccgatggt gctcactggc agcggcatgc tttgcactac
      781 cgctaacgcc gaagagtatt atttcgaccc cattatgctg gaaactacaa aaagtggtat
      841 gcaaacaacc gatctgtcac gtttttcaaa aaaatacgca caactaccag gaacttatca
      901 ggttgatatc tggctgaata aaaagaaggt ttcacagaaa aaaattacat ttaccgccaa
      961 tgcagagcaa cttctgcagc cacagtttac ggtagaacaa ctacgtgagc tgggtattaa
     1021 ggtggatgaa atcccggcgc tggctgaaaa agatgacgat agcgtgatca actcgcttga
     1081 acaaatcatt cccggtacag ctgctgaatt tgatttcaat catcagcgac ttaatttgag
     1141 cattccccaa attgcactgt accgtgatgc aagaggttac gtctcccctt ctcgttggga
     1201 cgatggtata ccaacgctgt ttaccaacta ctcgtttaca ggttctgata accgttaccg
     1261 ccagggcaat cgtagccaac gacagtacct gaatatgcaa aatggtgcta attttggccc
     1321 ctggcgatta cgtaactatt ctacgtggac acgcaacgat caggcgtcaa gctggaacac
     1381 tatcagtagt tatttacaac gtgatatcaa ggcgttgaag tctcagttgc ttctgggaga
     1441 aagcgccacc agcggcagta ttttttccag ctacgccttt actggcgtgc aactcgcttc
     1501 cgacgataat atgttgccaa acagccagcg cggatttgcc ccaacggtac gcggtatcgc
     1561 aaacagtagt gcaatcgtga ctatcaggca aaatggttat gtgatctatc aaagcaacgt
     1621 gccagcgggt gcctttgaaa ttaacgatct ctacccctct tccaacagcg gcgatttaga
     1681 agtcacgatt gaagaaagtg acggtacaca acgtcgcttt atccagcctt attcttcatt
     1741 acccatgatg cagcgacctg ggcatctaaa atatagcgcg accgctggac gctatcgcgc
     1801 tgatgcaaac agtgatagca aggaacccga atttgctgaa gccacggcaa tatatggttt
     1861 gaataatact tttacgctgt atggcggcct gctcggttct gaagattatt atgcgctggg
     1921 gatcggtatc ggcggcacac ttggcgcact gggcgcgttg tcgatggata tcaacagagc
     1981 tgacacccaa ttcgataacc agcactcttt tcatggctat caatggcgta ctca
//
LOCUS       DAJTUP010000234         2031 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTUP010000234 DAJTUP010000000
VERSION     DAJTUP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.238"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..546
                     /locus_tag="OO468_004086"
     CDS             <1..546
                     /locus_tag="OO468_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6260969.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
     gene            958..1326
                     /locus_tag="OO468_004087"
     CDS             958..1326
                     /locus_tag="OO468_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260970.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            1757..>2031
                     /locus_tag="OO468_004088"
     CDS             1757..>2031
                     /locus_tag="OO468_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260971.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDS"
BASE COUNT          479 a          535 c          492 g          525 t
ORIGIN      
        1 tatcagttca cacgacacac ggctcacacg acctctgagg cccgtagatg ggcgtctctg
       61 gtgtctgtgg tcactggcgc tcggtctgct gaggtctgcc acctgactaa acgtgacatt
      121 gtaaccttgg acaatggact ggtctgtatc gacatcaatg aagatgggga cgggaagtct
      181 gtgaagaaca aacattcagt ccgacttgtg ccgttaaccg atggcgctta tgggtttgac
      241 ctgacgtcct tcctgtcgtg ggtagacatg cagcctgatg agggtcctct cttcgggatg
      301 actccgagcg cctactctag ctggtttaac tcaagggtcc tgactgaggc cctaggagac
      361 tctcaggatg tctctctcca cagtctgaga cattggctgg cgacccgaat gaaagagcgt
      421 ggagtcaatc tggtggacgc tcaaggcatc ttaggtcaca gcagtcagtc aattacgtat
      481 gacctgtacg ggaaaggaca cgctgtgggt aggctggctg atgtgcttaa gacagcttta
      541 ctttaagtag ggagacccaa ggtggagacc atatacggct tgatggttga gaccttgggt
      601 cactgaagtc tgactgaagt tagggagggt gataataatc ataatccctc ctgactatag
      661 gcccactggt ccgccttcgg ctgtccgact ctaagtctgt ctgtggtctt atttaaactg
      721 cgtcctaaac tccagtccct ccaaattcct atggtctcct cttgacattc ctgtttaact
      781 gtgagtcgcc tctggtctct ctttatatat acttccttaa ttcctgaggc gtctttagtc
      841 cttgacattc ggttcaacct gtgagtcaca tcctgtcgcg ctactgtatg catcaccagt
      901 gatggacttg attatcacta taggggaacc aactaattca actacaggag atactctatg
      961 gcacgaccaa tcgcaactca cgacaacacg ttcaccaaag cctacttgca gcagcactgc
     1021 ggggacctgc tgtcattcga tggtcagggc gacctgagtg gttggctgga tgatgtcctt
     1081 actggtgctg gtcgcctgaa cgaatcaatg gcaagcaata ccaagcctgt cagcccatac
     1141 ttgattctga cccaactact aacgcacgac acattaactg taagcgctgt gcaggagtcc
     1201 ctgagccgta agcgtgttgc cttgggtgaa cctatggttt caactcgcta cgctcgctac
     1261 gtctatgcta ccgtcgtcag cgccagtaaa tccgtccagt accacgccag taaagctggt
     1321 tcatgattac ggaccctaaa ctatcactat agggagacca gcctccgcct ctgagtctga
     1381 tgcgtcagaa catgcgagca tctggtcaac cgacattaag tatctctcct aagtaatcaa
     1441 ggcccgtgac cgcctctgac agacctcctg tatcgacctc cagctcgtta gcgttcttta
     1501 gtcgtctcgg ttagtagctc gtctactcac tacattggct taagtctatc accgctcacc
     1561 atctggagtc cttacagctc gtcctgacat gcggtcgcac ctttatacgt acatggagaa
     1621 catggtcact ccagaccgtc aacatttaac cattcacttt cgttaagcac tttctgatgg
     1681 gtccgtttag gatgcgtctg aatggtgcgg actccatgtg gagacagaaa gtgctttacc
     1741 aaaggagcat ctttgtatga ataccgaatt accagtaact aaccgccagt tcttaatcat
     1801 cctgctgacc aatgcagagt ccagtgtcga ttgctgtcgc tataacttag acctgctgaa
     1861 gttatcccga tggtatgccc aggcggaaga ctgggactat gagaaaatcc gggagatgta
     1921 cgcggagcct atccagtcat acctccagac acacaccgag gaagagctga agcgtggcct
     1981 gcaggacatc ttctatttca acaatcctta cacctgtgag ggtgactcag a
//
LOCUS       DAJTUP010000235         2000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTUP010000235 DAJTUP010000000
VERSION     DAJTUP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.240"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..515)
                     /locus_tag="OO468_004089"
     CDS             complement(<1..515)
                     /locus_tag="OO468_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260972.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQD"
     gene            complement(1177..>2000)
                     /locus_tag="OO468_004090"
     CDS             complement(1177..>2000)
                     /locus_tag="OO468_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260973.1"
                     /translation="AQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEV
                     VTAPSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSK
                     MVDVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHL
                     KALSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESI
                     TVVLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
BASE COUNT          551 a          456 c          468 g          525 t
ORIGIN      
        1 atgtcctgca tccgcttaat ctggtcgggc gtagacatat aaccccacag ggtgagcaga
       61 accttttttg cggctacact atttccttgg tctgatacta atgccatata gccccataaa
      121 ctatccctca gccagacagc atcatagtgg gtttctgccg ctgttttgtc atcttcgaaa
      181 gtagaagcaa gaataaacgt tccccagggt ttctccgtca acgttaactt tcgtgtgttt
      241 tctgttaatg ttagcaatcc ttgagtttcg tctttggtat agtgactctt tatttcattt
      301 tttaatttgg ctgagtgaat cagaagacga taattaggat acttatctgg atttaacgat
      361 aaataacttt tatctgttgc tgcccccctg gaattaagaa tgttaaatac agattcgctt
      421 gtcacttctg cctctgtcgc ctggcatgtg ccgcacgaaa atataccagc gagcagaaga
      481 gttaatgcag atttcccttt cataaaataa tccatcaatc gtttgttaaa agcgtagata
      541 accacttatt caacaacttg aaaataaggt attttacctg agttgttaca aaaggattgc
      601 attgcgtaaa cgctttttat ttacaaaaaa atggggaagt gttacagcga ttgcgaaaag
      661 cctgaagtga ttgaattaag ataatataga aaatcagata cagcaaaaat ggcagattga
      721 tcaggtgaag ataaattgga gcgggcgaag ggagcctacg acaaatcgaa gacaaccagc
      781 taacctattg tttcttaaag acaggtgaat ccagttggtt gtagtgatag tcacacattt
      841 agagatagaa ggtcaagact tgaagcacgt aatattccct ctaaaggaat agaactcacg
      901 gtctgactac ccggcccttt tggtctggct caggtcaggc tatgggggct tttggctcgc
      961 tctttatatt agatgaggtc tcacattttt cagtcaaaac tcatcgtcta atagcccata
     1021 gccagaccac cagtcccagc tttagcagga agaccacgag atagactttg tgtaggtcca
     1081 tttggccctc ctactttctg tattcacaaa ctagattact agtacaaaat aaaggccaga
     1141 catccagttc ggaagtctga ccaatagtcg ttattgttag ttagcttctt tagtgaagct
     1201 caggcgtttg actacaggca ggacagcagt agccgagcca gtacccatct tgatggtcgt
     1261 agcagcttca gctacaacaa acaccttgcc gagaatctca aggtcgtgag actgggagtc
     1321 tccatcacgt gaaaggacca cagtaatgct ctcgccatcg tgacggaagc aaccgaagct
     1381 cagagccgta aaacgcttag ccatcatgaa cacgccacgg tcttcaccgt tgtaataggc
     1441 gacgtcagtc cccagcatat cgacgatagc gtcttcacca ccgaagccct gcttagcagc
     1501 cagacgggag agagccttga ggtgtgactc atgggcgata atgccgtagt cagaaacctc
     1561 agaaccgaaa gaagactccg tgttctcgta cagggcatcc atgatagcgt cagccttagc
     1621 ggtcaggcta ccagtcaggg ccggaagttc tacggtcttc agcagctttg aggactccag
     1681 cacgtcaacc atcttagagt taatggtacg aatcacgtta tcgcagatgt tcagtgcgtc
     1741 ctgctggagc gtcccctgag tgaaaccagt ggtcgacttc tggtcccaaa gctttgtttg
     1801 gtgagacacg aggatcgcct gttcaatcgg ctcaacggtg atttcagtgc cagacggagc
     1861 ggtcacaact tcaggtcgct taccaccacg gacagggacc agcacagagc ggtcagccag
     1921 agtgtgagca atgattggag tcggtaagct gttacgggtc ggcaggactt ctacagggaa
     1981 cgggatgtta tcttgagcgt
//
LOCUS       DAJTUP010000236         1954 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTUP010000236 DAJTUP010000000
VERSION     DAJTUP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.242"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..121
                     /locus_tag="OO468_004091"
     CDS             <1..121
                     /locus_tag="OO468_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR6260974.1"
                     /translation="GRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            145..435
                     /gene="lsrG"
                     /locus_tag="OO468_004092"
     CDS             145..435
                     /gene="lsrG"
                     /locus_tag="OO468_004092"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR6260975.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            474..1232
                     /gene="tam"
                     /locus_tag="OO468_004093"
     CDS             474..1232
                     /gene="tam"
                     /locus_tag="OO468_004093"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR6260976.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1236..>1954)
                     /locus_tag="OO468_004094"
     CDS             complement(1236..>1954)
                     /locus_tag="OO468_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260977.1"
                     /translation="FLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKTTLPDSASV
                     REFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRILLIMGEWLA
                     VQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTVYLFCIMLP
                     FALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICNAIEIDLLQ
                     MNDEAEIPAKILPDRHYQLT"
BASE COUNT          514 a          475 c          502 g          463 t
ORIGIN      
        1 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
       61 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      121 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      181 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      241 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      301 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
      361 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
      421 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaatgtctg
      481 actggaaccc ctctttatat ctgcactttt ccgctgaacg atcgcgtccg gcggtggagc
      541 tgcttgccag agtgccgctg gagaatgtcg aatatgttgc cgatcttggc tgtggcccag
      601 gtaacagcac cgccctgctg catcaacgtt ggcctgcggc caggataaca ggcatcgact
      661 cgtctccggc gatgattgct gaagcgcgca gtgctttgcc agactgccag ttcgtggaag
      721 cagacatccg caactggcaa ccggaacagg cactcgatct gatttttgct aatgcctcac
      781 tgcaatggct gcccgaccac tacgaattgt ttcctcactt ggtttcttta cttaatccgc
      841 agggcgtgct ggcagtacag atgccagata actggctgga gccgacccat gtgctcatgc
      901 gcgaagttgc ttgggaacaa aactacccgg atcgcgggcg tgagccgttg gctggcgttc
      961 atgcttacta cgatattttg agcgaagccg gatgtgaggt cgatatctgg cgaaccactt
     1021 actatcacca gatgccgtcg caccaggcga ttatcgattg ggtgaccgcc actggattac
     1081 gtccgtggtt acaggatctg accgagagcg aacagcagct ttttcttacg cgctaccatc
     1141 agatgctgga agagcagtat ccactgcaag agaacggaca gatactgctg gcatttccgc
     1201 gtctgtttat tgttgcccgc cgtacggagt aattatcacg tcagctggta atgacgatcg
     1261 ggaagaattt ttgctggaat ttctgcttca tcgttcatct gtaacaggtc aatttcaata
     1321 gcgttgcaga tggcatccag cggtaaatca ttgttttcag taccgaacgg atcttccagt
     1381 tcttccgcca gacaatccag cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc
     1441 gtcatgtaat gcaggtccac gaccagcgcg aacggcagca tgatacaaaa cagataaacg
     1501 gtacgatgca aaatcagggt gtaggcaaag ggaattggcg tgtaggcaat gcgctcacag
     1561 cctgccagaa ccgctgaaat atcattaagc cgatcgttga ggctaataaa caggatatct
     1621 gaaagctgtc cattgcggcg ctgaaccgcc aaccattctc ccattattaa caagatacgg
     1681 ttagctggag agttcgaagc cagtacacgc tgaagatctc cagtcttgag ataatgagcc
     1741 agcacttccg cctgtggctg tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt
     1801 tgcagcctgg caaactccct tacacttgcc gaatccggca atgtcgtttt tacctctcgc
     1861 agtaacgacc gtgaggcaat catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg
     1921 gcgtacccgg cattattacg aaaaccaaga aaaa
//
LOCUS       DAJTUP010000237         1941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTUP010000237 DAJTUP010000000
VERSION     DAJTUP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.243"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..948
                     /locus_tag="OO468_004095"
     CDS             <1..948
                     /locus_tag="OO468_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260978.1"
                     /translation="TDSWFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCF
                     GSSTIEYFSLTGATTVGAALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPS
                     VYIIDSGQVSPIASASIEKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDA
                     SSIANGPQWCVLKTGLYDDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQ
                     EHLLFTPLFKADNARVFDLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFV
                     YDKRVLWKRVGRIRKNVGFKLRVITKSPVTLSGAQIRIE"
     gene            948..1796
                     /locus_tag="OO468_004096"
     CDS             948..1796
                     /locus_tag="OO468_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260979.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            1796..>1941
                     /locus_tag="OO468_004097"
     CDS             1796..>1941
                     /locus_tag="OO468_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6260980.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQ"
BASE COUNT          495 a          501 c          477 g          468 t
ORIGIN      
        1 actgattcat ggtttatcac tgaccttgaa gacgaatctc atcctgaccg ttacagcgca
       61 caatatcgtg ccgagtctca gcctgacggc atcatcggta tcgggacatg gcgagacttc
      121 atcgtctgct ttggttcatc gactattgaa tatttctccc tgactggcgc aaccaccgtt
      181 ggtgccgctt tgtatgttgc acaaccatcg ctgatggtgc agaaaggcat tgccggaact
      241 tactgcaaaa cgccattcgc tgattcgtat gcgtttatca gcaatccggc aacgggtgcg
      301 ccgtctgtgt atatcatcga ctccggtcag gtgtcaccaa ttgccagcgc gagcattgag
      361 aaaatcctcc gctcctacac tgctgatgaa ctggctgatg gtgtgatgga atcgctgcga
      421 tttgatgctc atgagttgct gattatccac cttccgcgtc acgtcctcgt gtacgacgca
      481 tcttcaatcg ccaatggccc acaatggtgt gtgctgaaaa caggtctgta tgacgatgtg
      541 taccgcgcta tcgacttcgt ttacgaaggc aatcagataa cgtgcggcga taagctggaa
      601 tctgttaccg ggaaactgca attcgatatc agcagccagt acgacaagca acaggaacac
      661 ctgctgttta ctccactctt caaagcggat aacgccagag tgttcgacct tgaggttgaa
      721 tcgtcaactg gcgttgcgca gtacgccgac cgcctttttc tctctgcaac cactgactgc
      781 atcaattacg ggcgtgagca gatgattgag cagaatgaac cgttcgttta cgacaagcgt
      841 gttttgtgga agcgagtagg gcgcatcagg aaaaatgtcg gcttcaaatt gcgcgttatc
      901 acgaagtcac ctgtcactct gtctggcgct cagataagga ttgagtaatg gctgattcga
      961 atctcaatgt gccggtaatc atccaagcta cgcggctcga tacatcagtc cttccacgca
     1021 atatcttctc gcagtcgtat ctgctttacg ttatcgcaca gggcactgat gttggtaacg
     1081 tggctaacaa agccaacgag gccggacagg gcgcttatga tgcacaggtc aggaacgatg
     1141 agcaggatgt gattctcgct gaccatgagc agcgaatttc tgctgcggaa gcaacgcttg
     1201 ttaatcatga ggagcgaatc agacaggcag aatcaactct tcaggaccat gaaacacgaa
     1261 tagctcagaa tgaaagcgat atttcgtcgc ttgacacaag agttcagtcg ctggagtcgc
     1321 aggtttcaga ccatgaaacg cgcatcgatg ctctggagta tgcaaccaca cgcaagaagt
     1381 cagaggttgt ttactctggt gtatctgtaa ccatcccgac agcgccgacc aaccttgtta
     1441 gcctgctgaa aacgctcaca ccgtcatccg gctcgttggc accattcttc gacaccgtta
     1501 acaacaagat ggttgtgttc aacgagaaca aaaccctgct cttcaagttg tcgattgtcg
     1561 gcacgtggcc cagcggaacc gccaacaggt caatgcagct aaccttttcc ggctctgttc
     1621 ctgacacact ggtcagcagt cgtaatgcgg cgacaacaac cgacaacatc ctgttagcta
     1681 cgttcttcag cgtggataaa gacggctttc ttgccacaaa tggcagtacg ttaaccattc
     1741 agtcgaatgg tgcggcgttt actgccacaa ccatcaagat aatcgcggag cagtgatgat
     1801 tacattcaaa ccaacgcgaa acatcgacct gatagaagca gtcggaaatc accctgacat
     1861 tatcgccggg agcaacaacg gtgatggata cgactacaaa cctgaatgcc gttactttga
     1921 ggtgaacgtg cacggtcagt t
//
LOCUS       DAJTUP010000238         1941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTUP010000238 DAJTUP010000000
VERSION     DAJTUP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.244"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            82..831
                     /locus_tag="OO468_004098"
     CDS             82..831
                     /locus_tag="OO468_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR6260981.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            828..1397
                     /locus_tag="OO468_004099"
     CDS             828..1397
                     /locus_tag="OO468_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260982.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            1521..1763
                     /locus_tag="OO468_004100"
     CDS             1521..1763
                     /locus_tag="OO468_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR6260983.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            1767..1913
                     /locus_tag="OO468_004101"
     CDS             1767..1913
                     /locus_tag="OO468_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260984.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
BASE COUNT          513 a          459 c          516 g          453 t
ORIGIN      
        1 ccagcggtgc caacacacca tttattcagg ttaagtaata catatgcccg gtattacacg
       61 ccgggctgtc tggagataaa aatgaatact gtaaccatca acaacaaaca gtttccggta
      121 atcgaatatc gcggtcagcg tgttgtgacg ctggcgatga ttgatgaagt ccaccagcga
      181 ccggaaggaa ccgcgcgtgc agcattcaac cgaaaccgtg agcatttcat cagcggtgtg
      241 gattatgccg aattaggtgc ggacgtaata cgtacggacc tcccggaagg aacattctct
      301 aaatttgcac catcggggat tgtacttttt gaatcaggtt acctgatgtt gacgaagcca
      361 ttcaatgacg cccttgcctg gcaggttcag cgcgaactgg ttaacagcta cttccgcact
      421 cgcacaccgc taacggaaat cgagatgatc gctgcgatag ccgccgacgc tgttcgtcaa
      481 cagaagcgcc tgaatcatgt ggaagaacag ctcgaaacag tcacagaagc ggtggaaacc
      541 atcaaacgcg gaaatatgcg cgccggatat gtcggttacc gccaggtggt agccaaaagt
      601 ggaatgagtg acaccaagtg tcggaatctg gtgaacgcat atcaaattcc tacagacacc
      661 cacgaattca tgactccgga cggtctgttg tctcgcaggg ctatcgtcga gcttgagcca
      721 tttatggcgg cgttccacca aatgatgtca gaggctgacc cacgcggcac acgctggtat
      781 cacccgaaga tggggctttt ccaggtgatc ggatgggagg ataaagcatg atcatccagt
      841 caaaacttat tcgcgccgct ctggtgtgcg ctgctaaaaa cgacgttcgt tattacctga
      901 acggtcttca catcacgccg aaacatattg aggcaaccaa tggttccgtg gcactgcgca
      961 tggctcacgg catccggacg aagaaaaaca tcattgtcca gttcgaaggg ggcgtcccgg
     1021 ccaaagccga aacgacagag ctgattttta gtaaagagcc gatcgctgtt catcgcgacc
     1081 agtttcagcg acgactgtcc attaccggca ttaaactggt ggacggttgt tttccggatt
     1141 tggatcgcat cattccgaaa aagtttgacc gctgtactca cccggtgctc caggcgggtt
     1201 acctgagtta tccagagaag atgtttggtc gtgagcgtaa atttattccc gtccagttac
     1261 gtccctccgg tgacgggcaa gcggtcagaa ttcagtttga ttccatcatc aactcaatgt
     1321 atggcaatcc tgaatttgtt gtgatgcctt gtcgtgatca tggcgatttc aacgtggctc
     1381 aggagcatcc ggaatgaaaa tcgaatacca ggacgccgca ggaggtgaat caatgagctg
     1441 gcctgatgca atcgtaactc tggggatggt attcgcagta gcgtttgttg tgtactcgat
     1501 ttgtcgatgg ggataaccac atgttcgctt tgattcaacg cgggcagata tacacagaca
     1561 gagccggata cccagtagaa attactcgca gtactgagca ctcagtgttc tttcggagga
     1621 tggacggacg taccgggcga gtacgcatcg gtgaattcag tagcctgttc gagcatattg
     1681 accacctgga atatcacaag attctggctg aaacagaaca agaaaagcac ctgaagaaat
     1741 tacgcgcaat gcaaaggaag taaaaaatga ataaagcgtt tgagctatgg gtccaccagc
     1801 gttacggcaa ccactacgac ctgacgcgag atgttgacgg tttctactgt cgtgaaattg
     1861 tgaaacgaat gtttgaggtc tggtgccact gccgtgggct gagtgttgtg tgaggtaatg
     1921 catgggcaat gtgattcaac t
//
LOCUS       DAJTUP010000239         1925 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTUP010000239 DAJTUP010000000
VERSION     DAJTUP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.245"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(103..267)
                     /locus_tag="OO468_004102"
     CDS             complement(103..267)
                     /locus_tag="OO468_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260985.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1169..1453
                     /locus_tag="OO468_004103"
     CDS             1169..1453
                     /locus_tag="OO468_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6260986.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1441..>1925
                     /locus_tag="OO468_004104"
     CDS             1441..>1925
                     /locus_tag="OO468_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6260987.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          490 a          410 c          554 g          471 t
ORIGIN      
        1 tgtctgcaat accccgggta ttttcgattt gcgaaatgga tggaacgact tgctcagggg
       61 atagctgatg gagttactga agtaccgaag gaccactaaa aatcacaggc cgggaatttc
      121 ggcgtttccg gcgtaggtgt gccacgaatg catccagtgg cgtgatgttg gatgcaatgc
      181 tgtatgagag atgaggggcg gcgtccagac gcagccttca aaccgccttg agctgctgcc
      241 gttaagagcg gtggtggtga gcgtcaaggt taaaaggcgc agaacatgtg tcaggtaggt
      301 gttgcggaga tgaacgaaag tgaactgtcg atgaagtgtc gaaaggaacc tctaggcgat
      361 gtcgaaaatg tgctatctac gcagcgcatg acgagtccag cagatatctg gtactggctg
      421 ggcggcatcc ggcatatagg cagcatgaac gcaacacagg ctctcgtacg gaacgtggga
      481 acctgtcgtc tcgatgctaa gggagcggcg cggagcagag gcactgcaag cgcctgagta
      541 ccgatgcgag acacaggggc agatggcttc gtagtagtga ggaagtgcct gtaatgggca
      601 tggagcgaag ggggtcagtc gttggggctg gtttatcggt caaccggata gccgggagga
      661 gcccatgagt cagccaaagc cgttcgaggt ctctaagtat gcggtttgga aagcatacca
      721 gcgggtcaaa gctaatcgag gtgcggcagg tgtcgatggg cagtcagtcg aggcgtttga
      781 ggttaccaat cgaagctata cccggtaatg gcgcaggttg acctgcactt ggccaagtgg
      841 attgcccgca aacacaagcg agcgcgaggt agtctggtgc gtgcattcga gtggttactg
      901 cgaattcgtc acgactgccc gacgctgttt gcacactggt gcttagctta cgatacttga
      961 atggttcaac gacaagagcc gtatgacgcg agagtgtcac gtacggctcc gtgggagcgt
     1021 aggggggcgg tgcccctgcg ccacccgata tcccctttga gtgcggttac gtccacagag
     1081 gtaaatgccg ctgacgcggc atctgtaatc tgttactttg tgtatatcat ttgctatata
     1141 ttgtcaatat gctattgttg taaagcaaat gatatacgga ggctttatga aatcaggtgt
     1201 tcagctcaat ctcagagcaa gagaatcaca gcggatactc attgatgctg ctgcagaaat
     1261 tcttcataag tcgcgtacag atttcattct ggaaatggcc tgtaaagctg ctgaagatgt
     1321 tattctcgac cggcgggtgt ttaattttaa tgacaggcaa tatgaagagt tcattgaaat
     1381 gcttgatgct ccggttgcag atgatcctgc catcgaaaaa ttgctggcaa ggaaacctca
     1441 atgggatgtg taactgcacc agaaccttta tcctcatttc atcaggtcgc tgaatttgtc
     1501 agcagtgaag ctgtacttga tgactggtta aagcagaagg agctaaaaaa tcaggcaatc
     1561 ggagccaccc ggacttttgt tgtatgcaga aaaggtacgc aacaaatagt cggtttttac
     1621 tcactggcca ctggtagtgt caatcataca gaagctacag gtaaccttcg gcgcaatatg
     1681 ccagacccca ttcctgtaat tatacttgca cgcctggctg tagatgtgtc atttcgagga
     1741 aaagggcttg gcgcggattt acttcatgat gcagttcgtc gttgttacag ggttgccgaa
     1801 aatatcggtg tgcgtgcaat tatggttcat gcgcttacgg aaaacgcaaa acagttttat
     1861 attcatcatg gttttaagcc atcaaaaact caggtgcaaa cattgtttct taaactccct
     1921 caata
//
LOCUS       DAJTUP010000240         1921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTUP010000240 DAJTUP010000000
VERSION     DAJTUP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.246"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            49..>1921
                     /locus_tag="OO468_004105"
     CDS             49..>1921
                     /locus_tag="OO468_004105"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR6260988.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     "
BASE COUNT          476 a          509 c          497 g          439 t
ORIGIN      
        1 atttggtact atctaacccc ttttcactat taagaagtaa tgcctactat gactcaagtc
       61 gcgaagaaaa ttctggtgac gtgcgcactg ccgtacgcta acggctcaat ccacctcggc
      121 catatgctgg agcacatcca ggctgatgtc tgggtccgtt accagcgaat gcgcggccac
      181 gaggtcaact tcatctgcgc cgacgatgcc cacggtacac cgatcatgct gaaagctcag
      241 cagctcggta tcaccccgga gcagatgatt ggcgaaatga gtcaggagca tcagactgat
      301 ttcgcaggct ttaacatcag ctatgacaac tatcactcga cgcacagcga agagaaccgc
      361 cagttgtcag aacttatcta ctctcgcctg aaagaaaacg gttttattaa aaaccgcacc
      421 atctctcagc tgtacgatcc ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc
      481 acctgcccga aatgtaaatc cccggatcaa tacggcgata actgcgaagt ctgcggcgcg
      541 acctacagcc cgactgaatt gatcgagccg aaatcggtgg tttctggtgc tacaccagtg
      601 atgcgtgatt ctgaacactt cttctttgat ctgccctctt tcagcgaaat gctgcaggca
      661 tggacccgca gcggtgcgtt gcaggagcag gtggcaaaca aaatgcagga gtggtttgaa
      721 tccggcctgc aacagtggga tatctcccgc gacgcgccat actttggttt tgaaattccg
      781 aacgcaccgg gcaaatattt ctacgtctgg ctggacgcac cgattggcta catgggttct
      841 ttcaagaatc tgtgcgacaa gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa
      901 gactccaccg ccgagctgta ccacttcatc ggtaaagata tcgtttactt ccacagcctg
      961 ttctggcctg ccatgctgga aggcagcaac ttccgcaagc cgaccaacct gtttgttcat
     1021 ggctatgtga cggtgaacgg cgcgaagatg tccaagtctc gcggcacctt tattaaagcc
     1081 agcacctggc tgaatcattt tgacgctgac agcctgcgtt actactacac tgcgaaactc
     1141 tcttcgcgca ttgatgatat cgatctcaac ctggaagatt tcgttcagcg tgtgaatgcc
     1201 gatatcgtga acaaagtggt taacctggcc tcccgtaatg cgggctttat caacaagcgt
     1261 tttgacggtg tgctggcaag cgagctggct gaccctgagc tgtacaaaac ctttaccgat
     1321 gccgctgaag tgattggtga agcgtgggaa agccgtgaat ttggtaaagc agtgcgcgaa
     1381 atcatggcac tggctgacct ggctaaccgc tatgtcgacg agcaggctcc gtgggtagtc
     1441 gcaaaacagg aaggtcgcga tgctgacctg caggcgattt gctcaatggg catcaacctg
     1501 ttccgcgtac tgatgactta cctgaagcct gtactgccga aactgaccga gcgtgcagaa
     1561 gccttcctca atacggaact gacctgggat ggtatccagc aaccgctgct gggccacaaa
     1621 gtgaatccgt tcaaggcgct gtataaccgc atcgatatga aacaggttga agctctggtg
     1681 gaagcctcta aagaagaagt gaaagccact accgcgccag ttactggccc actggcagat
     1741 gatccgattc aggaaaccat cacctttgac gacttcgcca aagttgacct gcgcgtggcg
     1801 ctgatcgaaa acgcagagtt tgttgaaggt tctgacaaac tgctgcgcct gacgctggat
     1861 ctcggcggtg aaaaacgcaa tgtcttctcc ggtattcgtt ctgcttaccc ggatccgcag
     1921 g
//
LOCUS       DAJTUP010000241         1885 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTUP010000241 DAJTUP010000000
VERSION     DAJTUP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.247"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..771
                     /locus_tag="OO468_004106"
     CDS             <1..771
                     /locus_tag="OO468_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR6260989.1"
                     /translation="YRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKLP
                     AVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISFI
                     GVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTSL
                     ADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGIF
                     ATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            816..1046
                     /locus_tag="OO468_004107"
     CDS             816..1046
                     /locus_tag="OO468_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260990.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            1024..1524
                     /locus_tag="OO468_004108"
     CDS             1024..1524
                     /locus_tag="OO468_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6260991.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            1524..>1885
                     /locus_tag="OO468_004109"
     CDS             1524..>1885
                     /locus_tag="OO468_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260992.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTV"
BASE COUNT          490 a          446 c          500 g          449 t
ORIGIN      
        1 taccgcaaag caggccgcaa cctggtagat ggtgacatct tcgggcgcgt tcctgaagaa
       61 gcatatcgaa acggtactat tcagcgtcag attgctggct ttgatgaaat tcttcgctca
      121 ccgaaacttc cggcagttac caagtcaacc gcgactggtg taactgtttc tggtgcgcag
      181 aagtttaagc cgcaggcata cacccttgat accgatggta acaaagagaa cgtcgacaac
      241 cgtgttgcaa cggtgaccgt atcctccacc accggattta agcgcggcga caaaatcagc
      301 ttcattggtg tgaagttcct gtctcagatg gcgaagaatg tgctgactga tgacgcgact
      361 ttctcaatca cccgtgtgat cgatggtact cacatcgaaa tcacgccgaa gccgattgcg
      421 ctggatgacg cgtcactgac aaaagaagag aaggcttacg ctaacgtaaa cacctctctt
      481 gctgatacca ctccggtaaa cgttctgaac gtggcaacaa ccaccgctaa cgtgttctgg
      541 gctgatgact caatccgcct gctgtctcag ccgatcccgg taacccatga actgtttgct
      601 ggcatgaaaa cgtcttcctt cagcattcca ggtattggtg ttaacggcat cttcgcaacg
      661 cagggtgata tcaacactct gtctggtaag tgccgtattg ctgtgtggta ttcagcatgt
      721 gctgtacgac cagaggcaat tggtgttggt ctgcctaacc agaccgcgtg ataaccagag
      781 ggagcttcgg ctcccgtttt tatctggaga caagcatgac acacatgatc tttcgtcatg
      841 gcgacatgaa gaagtggaaa ggcgttggct acgactttga aatcgtgaaa gccgaagagc
      901 ttcaggaata tctggatgct ggctggtttt cacatcctga tgaccttttg aaggatgttg
      961 cagagccaga gccagaagaa aaacagcgta aaaagcctgg tcgaaaacct aaggcggcag
     1021 cagatgaacc tgacaacgaa gggtgattta gttcttgcgg cattacgtaa gctcggtgtt
     1081 gcatcaaatg ccacgttaac cgatgtcgaa ccgcagtcta tggaagacgg cgtcaacgac
     1141 cttgaaatga tgatggctga atggcttggc ggtgatgcgt cacctggtat caacgttggc
     1201 tacatttttg ctgatgcaga tgttgctccg gatccgggcg atgagcacgg tttatcaaat
     1261 aacgctatca atgccgtcat tttcaacctt gcctgccgaa ttgctccaga ttatgcgctg
     1321 gaagcgtctg caaaacttat aaccactgcc agatacggga aagagcgact cgtcaaactg
     1381 tctgcaatgg acagggcaaa agcagctaaa tgtaagtccg gttatccaaa ccgtatgcct
     1441 gttggtagtg gtaaccagtt ggcgaagtgg aatggttgga attacttcca ccgaaaggaa
     1501 ccttgcgata acgggagcga ataatgccta ttcagcaact tccgcttatg aaaggtgtcg
     1561 gcaaagactt ccgaaacgcc gactatatcg actatctgcc agtgaatatg ctggctacac
     1621 ccaaagaaat cctgaacagc agcggatatc ttcgctcatt cccgggcatt gccaaacgtt
     1681 ctgacgtgaa cggtgtatct cgcggcgtcg agtacaacat ggcgcagaat gctgtttatc
     1741 gcgtgtgtgg cggcaagctg tacaaaggag aaagtgaagt cggtgatgtt gccggaagtg
     1801 gtcgtgtatc aatggcgcat ggtcggacat cacaggcggt aggcgttaat ggtcaactgg
     1861 tcgagtatcg ctatgatggc acggt
//
LOCUS       DAJTUP010000242         1819 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTUP010000242 DAJTUP010000000
VERSION     DAJTUP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.248"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..292
                     /locus_tag="OO468_004110"
                     /pseudo
     CDS             1..292
                     /locus_tag="OO468_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245160.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            461..>1819
                     /locus_tag="OO468_004111"
     CDS             461..>1819
                     /locus_tag="OO468_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6260993.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEK"
BASE COUNT          472 a          349 c          409 g          589 t
ORIGIN      
        1 agtggttttg tccgctcaca ccagtacata caggtacgga aataaaacca gtcgaaatgc
       61 cggtaacgac gattactcct gtctctgatg ccggtggact acaagatttt atttactggc
      121 gtcctgatgc ggacagggct ggtatggaac ctgtttatgt gatacttaat gatcctctgg
      181 attccgggcg atttacgcgt aaacaactaa ataaaaatat cttaaacatg caagtaattt
      241 tggtattctc gatacaagaa taaatagtag taactactat agctgcaaat aatgttaaaa
      301 acatttctat gatcatcttt acatttttca tgattgacaa ttgtttttgg ttaaaaaaag
      361 acaactaatg agtgtgataa tgcaatactt tgagtgatta tataatgata agtaaacagg
      421 tttgcgacaa ttggttatca gtattacaga aggattaaat atgtcattat tcacagaacc
      481 taaggaaatt gaaagattac cctctgaaga gattgaacga ttatatcccg tcctgcgata
      541 cagggtcttt atcagcattt tcctcggata tatggggtat tattttgtcc gaaacaccac
      601 cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag atcggcatta tctcctgtgc
      661 cggtttttta tcctatggta tatcaaagtt tgtttccgga cttatttctg acagaagtaa
      721 ttcaaaagta tttttgtctc ttggtttatt cctgtccgga ctggtaaatt ttctgatagg
      781 atatattccc gggatcatca catccgttac gttattttca acaatgtatt tacttaacgg
      841 ctggatccaa ggtatgggat atcctcctgg tgccaaaaca ctggtgttct ggtatgaaca
      901 tcgggaaagg atcacctggg ccacactgtg gaatttatcg cacaatgtcg ggggagctct
      961 ggcccccgtt ctgattggtt tcagttttgg ttttttcggc gacagtgctc tggatcatgc
     1021 ccgggccgca tttatcttcc caggagtact gtgtatggca atgagtgtgc tgatttactt
     1081 tattcaggtg gacagacctg tatcagtggg gcttcctccc attgaagaat ggaaaggtaa
     1141 cgtcgtttca cacccggcta aaggccgtga acaaggtccg agactctcca ttcctgatat
     1201 catcagaaaa catatcatcc gaaataataa actcatttac tgctgtatct atggttcgtt
     1261 tgtttatatt ctgagatacg gtattgtttc ctgggctcct aagtttttaa gtgacagtct
     1321 ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg ggggggggtt ctgtttttga
     1381 aatcggtggt gtggctggta tgcttcttgc cggatacctg tctgtcagac tgttcagaaa
     1441 ctcaaaacca ttaacaaata ctttatttct ggcacttacc attattttat taatcgcgta
     1501 ctggtacgtg ccatcaggca atgaatatct ctggctaaac tatacaatcc ttattctgtt
     1561 ggggctggca gtgtatggtc cggtgatgtt tattggtctt tattcaatgg agcttgttcc
     1621 taaagaagct gccggagccg catcagggct gtcaggcaca ttttcctata tcttcggctc
     1681 aattgttgcg acactgggaa tgggacttgt tgtcgattat ctgggatggg gggccacatt
     1741 tatcgtactg atactgtctg ctgtttttgc catcattttc acactgatga gtcgtgaacg
     1801 ctcactggag ttcgaaaaa
//
LOCUS       DAJTUP010000243         1763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTUP010000243 DAJTUP010000000
VERSION     DAJTUP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.249"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(158..1372)
                     /locus_tag="OO468_004112"
     CDS             complement(158..1372)
                     /locus_tag="OO468_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR6260994.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          534 a          427 c          361 g          441 t
ORIGIN      
        1 ccataaataa taaaaccagg gtcactgtct gaatacgacg aatccgttgc ggtttattac
       61 ccgcaacact ggttgacaca tcaacgtcca tttttagctc cttgagcgga attaatcgat
      121 ttgatgacta ttcagaaaat aattaagcga accgttatta ccagttcaac aatgtgccat
      181 cttccagtcg tgcgacgggg agatactccg ggtcatacgg atatttcgcc gccagtttct
      241 catcgaaaga aatacccagc cccggttcat cgcctgggtg catataacct tcctcgaagc
      301 gccagctgta cgggaaaact tcaagcattt gttcggaata gcccatgtac tcctgaacgc
      361 caaaatttgg tacccatagg tcaaaatgca gcgccgccgc atgacaaaca ggcgataaat
      421 ctgaagggcc atgcgaaccg gtacgtacct gataaagcga tgcaaaatcg gcaatccgcc
      481 gcatcccggt aatgccgcct gcatgggtga tcgtcgtgcg aatatagcca atcagctgct
      541 cttcgattaa ctgcttgcag tcccagatac tgttaaacac ttcaccgacc gcaatcggcg
      601 tcacggtatg ctggcgaatc agacgaaaac attcctggtt ctcggcaggc gtcggatctt
      661 ccatccagaa caggcggtaa tcctcaatgc tcttcccaaa gcgcgccgct tcgatggggg
      721 tcaggcgatg gtgcatgtca tgcaacagat gttcgttaaa accgaactta ttacgcaccg
      781 cttcgaataa cttcggcgta aaatcgagat acttttctga agaccacaac tgctcttctg
      841 gccacaggcc tttggttgcc ggttcatacg ccagtccttt gcccttcgcc aggccataag
      901 tggtttgcat acccggtaca ccgcactgca cacgaatcgc cttaaagccc atctgctggt
      961 gctttgcata gtcttcaagg acttcatcaa tcgtacgccc ggtggtgtgg caataaacca
     1021 tcacgccttc acgagaagca ccaccaagca gttgatacaa tggcatattt gccgctttag
     1081 ctttgatatc ccacaacgcc atatcaatgg cagaaatggc tgacatcgtg acggggccac
     1141 gtcgccagta agcaccttta taaaagaatt gccagatatc ttcgatacgg ctggcatcgc
     1201 ggccaattag ttgtggacaa agatgatctt tcagataaga agcgaccgac aattcgcgac
     1261 catttaacgt ggcatctcct aatccacaaa gtccgctttc agtggttatt ttaagcgtaa
     1321 cgaaattacg ccctgggcaa gtaacaaaaa catctgcagc aataattttc attattgcaa
     1381 tctcctgtgt tgtaaataga cgtttcaaac ctaattttac gcctactacc atacaagaga
     1441 gaaaaagcga ttattcaacc gtgaccacat taaaaacaaa aatttctacc gaggagaaaa
     1501 aataccctca aaaaccacct gaataattaa ccattattgt taaaacaaca cgttgaataa
     1561 tcacataaca aactccatta atcattgaaa atgagcacag aaatagaaat taattatatt
     1621 caattaatta aaatgtgtat tgcttgatgt atttaaaaat attattttca tggtgagagt
     1681 ggaggtaaag aagacacaat aataaaaagg aatgtgacaa ataatggttc tggttgggcg
     1741 ttaaatttaa ggtcaccaga atg
//
LOCUS       DAJTUP010000244         1762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTUP010000244 DAJTUP010000000
VERSION     DAJTUP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.250"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..291)
                     /locus_tag="OO468_004113"
     CDS             complement(<1..291)
                     /locus_tag="OO468_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HCR6260995.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKK"
     gene            complement(483..>1762)
                     /locus_tag="OO468_004114"
     CDS             complement(483..>1762)
                     /locus_tag="OO468_004114"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226111.1"
                     /note="formate acetyltransferase; catalyzes the formation
                     of formate and acetyl-CoA from pyruvate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional pyruvate/2-ketobutyrate formate
                     lyase"
                     /protein_id="HCR6260996.1"
                     /translation="TESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEK
                     LPLNFKKFAAKVSIDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARA
                     NLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITAL
                     NIIHYMHDKYSYEASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDG
                     LAIDFEIEGEYPQFGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNV
                     VYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFS
                     IVPNALGKDDEVRKTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYP
                     QLTIRVSGYAVRFNSLTKEQQQDVITRTFTQSM"
BASE COUNT          422 a          432 c          460 g          448 t
ORIGIN      
        1 ttttttacag gcgcggaacc agtcacgaac aaactcagct tgcaggattg cttcaccgcc
       61 ggatgcggta acgccgccgc cggaagcgtt cataaagtgg cgataggtca ccacttcctt
      121 catcaaatct tcaacggtaa cttctttacc gccatgcgtg tcccaggtgt cgcggttatg
      181 acaatacagg cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa tacctgggcc
      241 gtctacggtt ccacaggatt caaaggagtg aatgcgacca ataactgaca ttgcggtgtt
      301 tctccagatg tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc
      361 tgttttggca gtcaccttaa agtatagata gctgacaaaa aaggctctcg tgctaaaaaa
      421 ggccccactt tcgtggagcc tttattgtac gctttttact gtacgatttc agtcaaatct
      481 aattacatag attgagtgaa ggtacgagta ataacgtcct gctgctgttc tttagtcagc
      541 gagttgaaac gtactgcgta gccagataca cggatggtca gctgcggata tttttccggg
      601 ttttccatcg cgtcgagcag catttcacgg ttcatcacgt taacgttcag gtgctgacca
      661 ccttcgatgg atgcttcgtg gtggaagtaa ccatccatca gaccagccag gttggtctta
      721 cgaacttcgt cgtctttacc cagtgcgttc ggaacgatag agaaggtgta ggagatacca
      781 tctttagcgt aagcaaacgg cagtttagca acggaagtca gagacgctac agcacctttc
      841 tggtcacgac cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg
      901 tctggggtgt taccagtttt cttaccatac acaacgttag aagtgatggt cagaacagac
      961 tgagtcggga tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct
     1021 accaggtcaa cagccaggtc atctacacgc ggatcattgt taccaaactg cgggtattcg
     1081 ccttcgattt cgaagtcgat agccagaccg tcttcgtcac gaatcggttt aactttcgca
     1141 tatttgattg cagacaggga gtcagcagca acggacagac cagcgatacc acacgccatg
     1201 gtgcggataa cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg
     1261 tgcatgtagt ggatgatgtt cagtgcagtg atgtactgtt tagccagcca gtccatgaag
     1321 tgatccatgc gctccatcac ttcatcgtag ctcaggagat cgcctttgat cggttcagac
     1381 ttcggaccaa cctgcatttt cagtttttcg tcaacgccgc cgttgattgc gtacagcatg
     1441 gttttcgcca ggtttgcacg cgcaccgaag aactgcattt gtttaccaac gatcatcggg
     1501 cttacgcagc aagcgatagc gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg
     1561 ttctcatact gcagagaaga ggtgtcgatg gacactttag cggcgaattt cttgaagttc
     1621 agcggcagtt tttcagacca cagaatggtc atgttcggtt ccggagacgg ccccatagtg
     1681 tacagggtgt tcaggaaacg gaagctgttt ttggtaacca gggtacgacc atcgaggccc
     1741 ataccaccga tagattcggt tg
//
LOCUS       DAJTUP010000245         1724 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTUP010000245 DAJTUP010000000
VERSION     DAJTUP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.251"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            276..812
                     /locus_tag="OO468_004115"
     CDS             276..812
                     /locus_tag="OO468_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6260997.1"
                     /translation="MAQNESDIASLDTRVQSLESQVSDHETRIDALEYATTRKKSEVV
                     YSGVSVTIPTAPTNLVSLLKTLTPSSGTLAPFFDTVNNKMVVFNENKTLFFKLSIVGT
                     WPSGTANRSMQLTFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTI
                     QSNGAAFTATTIKIIAEQ"
     gene            812..1267
                     /locus_tag="OO468_004116"
     CDS             812..1267
                     /locus_tag="OO468_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR6260998.1"
                     /translation="MIQFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            1270..>1724
                     /locus_tag="OO468_004117"
     CDS             1270..>1724
                     /locus_tag="OO468_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6260999.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGA"
BASE COUNT          468 a          439 c          439 g          378 t
ORIGIN      
        1 aggctacacg gctcgacaca tcagtccttc cacgcaatat cttctcgcag tcgtatctgc
       61 tttacgttat cgcacagggc actgatgttg gtaacgtggc caacaaggcc aacgaggccg
      121 gacagggcgc ttatgacgca caagtcagga acgatgagca ggatgtgatt ctcgctgacc
      181 atgagcagcg aatttctgct gcggaagcaa cgcttgttaa tcatgaggag cgaatcagcc
      241 aggcagaatc aactcttcag gaacatgaaa cgcgaatcgc tcagaatgaa agcgatattg
      301 cgtcgcttga taccagagtt cagtcgctgg aatcgcaggt ttcagaccat gaaacgcgca
      361 tcgatgctct ggagtatgcc actactcgca agaagtcaga ggttgtttac tctggcgtat
      421 ctgtaaccat cccgacagcg ccgaccaacc ttgttagcct actgaaaacg ctcacgccgt
      481 catccggcac gttggcacca ttcttcgaca ccgttaacaa caagatggtt gtgttcaacg
      541 agaacaaaac cttgttcttc aagctgtcga tcgtcgggac gtggcccagc ggaaccgcca
      601 acaggtcaat gcagctaacc ttttccggct ctgttcctga tacgctggtc agcagtcgta
      661 atgcggcgac aacaaccgac aacatcctgt tagctacatt cttcagcgtg gataaagacg
      721 gatttcttgc cacaaatggc agtacgttaa ccattcagtc gaatggtgcg gcgtttactg
      781 ccacaaccat caagataatc gcggagcagt gatgattcag ttcaaaccaa cgcgaaacat
      841 cgacctgata gaagccgtgg gaaatcaccc cgacattatc gccgggagca acaacggtga
      901 tggatacgac tacaagcctg aatgccgtta cttcgaggtg aacgtgcacg ggcagttcgg
      961 cggcattgtt tactatcagg agattcagcc gctgacatta gattgccacg ccatgtacct
     1021 gccagagatt cgcggcttca gcaaggaaat tgggctggcg ttctggcgat acattctgac
     1081 taacaccacc gttcagtgcg taacatcgtt cgctgcacgc aaattccgac acggtcagat
     1141 gtactgcgca atgattggcc ttaagcgtgt aggaaccatc aagaaatact tcaaaggcgt
     1201 ggatgacgtg actttttaca gcgccacacg cgaagaacta atcgacttcc tgaatcacgg
     1261 gagatagcca tgttatatgc atttaagctg ggcagaaaac tgcgcggcga ggaaccttat
     1321 tgccctgaaa aaggcgggaa aggtggcagt tctgataaaa gcgcaaagta tgcagcagaa
     1381 gcccagaagt atgctgcaga cctgcaaaat cagcagtggc agacgatcat gaaaaacctt
     1441 gctccgttca cgcctcttgc ggagcagtat gttaaccagc ttcagaacct ttccagttta
     1501 gaaggtcagg ggcaggcact taatcagtat tacaactcta agcagtataa agaccttgca
     1561 gggcaggcgc gttaccagag tcttgctgct gcggaggcga cgggtggact tggttcgaca
     1621 gccacaagca atcaactggc tacgatcgcg ccgacactcg gtcagtcttg gttatcaaat
     1681 cagatgagca attacaacaa tctggcaaac gttgggcttg gtgc
//
LOCUS       DAJTUP010000246         1705 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTUP010000246 DAJTUP010000000
VERSION     DAJTUP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.252"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1591)
                     /locus_tag="OO468_004118"
     CDS             complement(<1..1591)
                     /locus_tag="OO468_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR6261000.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQD"
BASE COUNT          381 a          463 c          453 g          408 t
ORIGIN      
        1 catcctgcaa cggatactcg ccaccgccgc cttcgcgggg aacaccatcg gcaatatggt
       61 atttctcgat cattttgtgc tgttccagat agaactgatt caccgttttc agccagctgc
      121 gcgcgatttc atcacccaga aggtcatcgc catacatttt aaatccctga attgccatcc
      181 attgtaacgg tgcccagccg ttgggtttat cccactgttc accggtttcg tactcgcttg
      241 ccaaaatccc gccaggtgtc agtaaccggc tgcgtacggc gtttgccaga cgatcggcct
      301 gttcatggtt cgccatgccg acatagagcg gcacaatggc ggcagcggaa aacagcgcca
      361 gttgttcgcg tcgccagtcg taatcgcggt agatgccgtt ttcatcatcc cagaggtaac
      421 ggtttaccgc atcgcgacgg gcactggcct tctggcggaa cagcgcttct gtctctttct
      481 cgcctttcag cgccgagatg ttggcgatgg cgctctccag tttaaacagg aaggcattca
      541 gatcgatggg gatgaactgg gtggtacgaa tgctcgccag acgaccagta tcacgcagcc
      601 aacgggaaga gtaatcccaa ccggaggccg ctcccgcgcg taaatcgcgg tacacctcgt
      661 tgggcgggcg accagaatgt ttcgcggttt caacgtcctc aagccaggat tcgtcacgcg
      721 gcgtgtcgcg atcgtcccag taacggttga gcagcgatcc gtccggcatc cgcacaacat
      781 ggcgataggc ctgattaggg atcaacgatt ctgcaccgtc catccagaag gcatattcca
      841 ttttaaggtg gtcgagatag cggcgcgcac cgcgtacacc atcttcttca aacaactcca
      901 ccatcagcgc aaaaaccggt ggttgcgagc ggctcaaata ataggtgcgg ttgccgttgg
      961 ggatgtgacc gtagttttcg atcatccagg cgaagttatc ggccatgcat ttcagcaaat
     1021 cttcccgacc actttccgcc agtcccagca tggtgaaata ggaatcccaa tagtaggttt
     1081 cgctaaaacg gccgcccggg acaatatatg actgcggtag cgccagcaga gaagaccacg
     1141 gaatgtgatc ctgtggttcg cgggttagca ccggccacag ctggtcgata tgctctttca
     1201 gggaattttg cgggtccgat acatactcgc tggagtagac ctccggcagc cagaagtgat
     1261 tttcaacaaa cttgcgcaag tcaaaatcac gatgacggcg cactttacgg tagcggatta
     1321 aaatatccag cgggtccatt ttgggtgcgc agtcgggaaa ggttttactg tcggggaaaa
     1381 ttttcgccga ctgaacatgc tcgaacagtt cgagatagcg atcggcaggc gtcagcgcat
     1441 cagaggcggg cagcccttca atcatctcgg gttccggttc tgcctcgatc atctcatcca
     1501 gttttaattc atacgggtcc agttcatagc agagatcgac ctcgatcatc agttcgtctg
     1561 gattagggtt ttgaattttc tgattgagca taaggatcca acctccgaaa ttcgtcgcta
     1621 attatcgggc ttttctttaa gggtagtcat tcgctccgcc tggtgggcga caaagcgtgc
     1681 ggtggatcac gtttattaac ttgtc
//
LOCUS       DAJTUP010000247         1698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTUP010000247 DAJTUP010000000
VERSION     DAJTUP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.254"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..964
                     /gene="ccp"
                     /locus_tag="OO468_004119"
     CDS             <1..964
                     /gene="ccp"
                     /locus_tag="OO468_004119"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR6261001.1"
                     /translation="EILAWIAKQRAEYYASNDTAPEHRNEPVQPIPQKLPTDAQKVAL
                     GFALYHDPRLSADSTISCAHCHTLNAGGVDGRKTSIGVGGAVGPINAPTVFNSVFNVE
                     QFWDGRAATLQDQAGGPPLNPIEMASKSWDEIIAKLEKDPQLKAQFLEVYPQGFSGEN
                     ITDAIAEFEKTLITPDSPFDKWLRGDENALTAQQKKGYQLFKDNKCATCHGGIILGGR
                     SFEPLGLKKDFNFGEITAADIGRMNVTKEERDKLRQKVPGLRNVALTAPYFHRGDVPT
                     LDGAVKLMLRYQVGKELPQEDVDDIVAFLHSLNGVYTPYMQDKQ"
     gene            1175..>1698
                     /locus_tag="OO468_004120"
     CDS             1175..>1698
                     /locus_tag="OO468_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6261002.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDV"
BASE COUNT          462 a          357 c          436 g          443 t
ORIGIN      
        1 ggaaatactg gcctggattg caaaacagcg cgcggaatat tacgccagca atgatactgc
       61 tccggagcat cgcaatgaac cggtgcagcc catcccgcaa aaactgccta ccgatgcgca
      121 aaaagtggcg ttgggctttg cgctgtatca cgatccccgt ttatcggctg atagcaccat
      181 ttcatgcgct cattgccata cgttgaatgc ggggggcgtc gatggcagaa aaacatcgat
      241 tggtgttggt ggcgcagttg ggccgattaa tgcgccgacg gtatttaact cagtatttaa
      301 cgttgagcag ttctgggatg gtcgtgcggc aacattgcag gatcaggcag gtggaccgcc
      361 gttgaacccg attgaaatgg cgtcgaaatc ctgggacgaa attattgcta agctggaaaa
      421 agatccgcag cttaaagcgc agttcctcga agtttatccg caaggtttca gtggcgaaaa
      481 tattactgat gccattgctg aatttgagaa aacattaatt acgccggatt ccccatttga
      541 taaatggttg cgtggagatg aaaatgcgct gacggcgcaa cagaaaaaag gctatcaatt
      601 atttaaagat aataaatgtg caacttgtca tggtggtatt attctcggcg gacgttcctt
      661 tgaaccgttg gggctgaaaa aagactttaa ctttggggaa attacggcgg cggatattgg
      721 tcgtatgaat gtgactaaag aagagcgtga taaattgcgt cagaaagtac ccggtttacg
      781 taacgttgct ttaacggcac cgtacttcca tcgcggtgac gtgccgacgc tggacggggc
      841 ggtgaaactg atgctgcgct atcaggtagg caaagagctg ccgcaggagg atgtggatga
      901 tatcgtagct ttcctgcaca gtctgaacgg ggtgtacacg ccgtatatgc aggataaaca
      961 ataattaatt tgatcgcccg aacaggaatg tttgggcgat ttttattacg ataataaagt
     1021 ctgtttttaa tattatcatg ttaaatgttt atattataaa aagtcgtttt tctgcttagg
     1081 attttgttat ttaaattaag cctgtaatgc cttgcttcca ttgcggataa atcctacttt
     1141 tttattgcct tcaaataaat ttaaggagtt cgaaatggac cagaagctgt taacggattt
     1201 ccgctcagaa ctactcgatt cacgttttgg cgcaaaggcc atttctacta tcgcggagtc
     1261 aaaacgattt ccgctgcacg aaatgcgcga tgatgtcgca ttccagatta tcaatgatga
     1321 attatatctt gatggcaacg ctcgtcagaa cctagccact ttctgccaga cctgggacga
     1381 cgaaaacgtc cataaattga tggatttgtc gatcaataaa aactggatcg acaaagaaga
     1441 atatccgcaa tccgcagcca tcgacctgcg ttgcgtaaat atggttgccg atctgtggca
     1501 tgcgcctgcg ccgaaaaatg gtcaggccgt tggcaccaac accattggtt cttccgaggc
     1561 ctgtatgctc ggcgggatgg cgatgaaatg gcgttggcgc aagcgtatgg aagctgcagg
     1621 caaaccaacg gataaaccaa acctagtgtg cggtccggta caaatctgct ggcataaatt
     1681 cgcccgctac tgggatgt
//
LOCUS       DAJTUP010000248         1692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTUP010000248 DAJTUP010000000
VERSION     DAJTUP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.255"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            85..753
                     /locus_tag="OO468_004121"
     CDS             85..753
                     /locus_tag="OO468_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261003.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(996..1691)
                     /locus_tag="OO468_004122"
     CDS             complement(996..1691)
                     /locus_tag="OO468_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR6261004.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
BASE COUNT          486 a          391 c          350 g          465 t
ORIGIN      
        1 tttgctgata tttaacccga acattcctga tacagactat taatacaaca ttatctgcga
       61 cagattttat tttctggata tattatgagt gaacaaatca aacaggatat cgatctgatt
      121 gagattttat ttcatctgaa gaaaaagatt cgtgttatcc tttttattat ggctatctgt
      181 atggcaatgg tgctgttgtt tctgtatatc aataaagaca atacaaaagt gatttacagc
      241 cttaaaataa accagacaac gccaggtata cttgttagct gtgatagcaa taataatttt
      301 gcctgtcaga ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcagacc
      361 agcccagata tcaagaacag agaaataagg ctggaatggt caggagataa gagagattta
      421 ccaactgctg aagaggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca
      481 gaataccata atggcaggca agttctcgat gagatacaaa cgccttcagc aattaacagt
      541 gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat
      601 ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc catttgcctg
      661 gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg
      721 gtagatgaat accaacaaaa ctctgggcag taataaacag cattctgata gagagtgcct
      781 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
      841 ctttttcact gtaaatatcc tggcattact aatgcctgga tatttacaga agagcagcat
      901 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
      961 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     1021 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgag
     1081 ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg cgctttctga
     1141 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     1201 agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc
     1261 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctct
     1321 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     1381 tgcaaacgtt cactaatccc caattcctcc agcctcgtgt caccgctaat cacgaccgac
     1441 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     1501 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     1561 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     1621 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgctc
     1681 cgattatcca tt
//
LOCUS       DAJTUP010000249         1680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTUP010000249 DAJTUP010000000
VERSION     DAJTUP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.256"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..146)
                     /locus_tag="OO468_004123"
     CDS             complement(<1..146)
                     /locus_tag="OO468_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HCR6261005.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGN"
     gene            complement(121..921)
                     /gene="otsB"
                     /locus_tag="OO468_004124"
     CDS             complement(121..921)
                     /gene="otsB"
                     /locus_tag="OO468_004124"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR6261006.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1088..>1680)
                     /gene="araH"
                     /locus_tag="OO468_004125"
     CDS             complement(1088..>1680)
                     /gene="araH"
                     /locus_tag="OO468_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR6261007.1"
                     /translation="ITTLATMQIVRGLAYIISDGKAVGIEDESFFALGYANWFGLPAP
                     IWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVVRTKIIIFVLSGLVSAI
                     AGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKISYVVAGILILGTVENA
                     MNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
BASE COUNT          420 a          466 c          395 g          399 t
ORIGIN      
        1 tcattccctg tttcaccact ccagccaaac cacagtccgc ctgcggcttt cagtgccccc
       61 agtatgccaa cggcaaggcc accggcactg gcggcgtgct cgtctggtgg tgcaatccgg
      121 ttagatacta cgactaaacg actcatagtc atcactcctg ttattttctc ttttttgttg
      181 taatgcggcg gttatcattt caagccagct ccagacatcc ggcacacccg ccagtcgcca
      241 tgatgcctga gttgcacctg tgccaatttt tactgacatt ccgcccagtc ggttaacgac
      301 tgcgaagcca gattcatcgg ttaaatcatc gcccagaaat acgggcgttc gcccgataaa
      361 aggagcttcc tgcataaagg ctgcaatcgc ttcaccttta ctggtacctc tcggtttgat
      421 ctcgacaaca cactttccct gctgtaacgc catttgtggc cagatctgag taatacgttg
      481 cgctaatgtc attaatgcgt cttcatgctg cggagcctga cgataatgca gcgcaaaagc
      541 catccctttc gcctccagct ccgcgccggg atactgagcg atgaccgtat gcagttgcac
      601 gctaatatca cgcgcaatcg catccggcag atgaacgata tgtgttttac cattgatgtc
      661 acggcgctcc gccccatgca cgcccgctaa cgggaagcga taaggttttg ccagtgcgtc
      721 aagctccacc attgagcgcc ctgatatcaa tgccaatgca ccatcacttg cggttgccag
      781 tagctgtagt ccttgcagaa tattgtcagg cacgacgacc tgatcgggat gcggtttgat
      841 ttccgccagc gttccatcaa gatcaaaaaa ccaggcatat ttcgcggata gttcaggggt
      901 ttcggttaac ggttctgtca cccggctctc ctccttctct tttcttattg tgttgcgcag
      961 accgcgcttt acagacatgt aagtatagac agtgtgacgg aggtcgccat ttgcaacgaa
     1021 gaacagccaa ccgttgtggc ggctggctgt ggtgggaaaa aacgttaagt tgttgtggaa
     1081 aaaagcatca gacagtgcgc ttcgcttttt gcttgtaacg gtcgaagatc accgctgcca
     1141 gcaggattaa gccgcgaacc acgtactgcg cgaaaggaga aatattaagc aggttcatgg
     1201 cgttttccac ggtgcctaaa attaagatac ccgccaccac atatgagatt tttccgatgc
     1261 cacctttcag agaaacgcca cctaaaacgc aggcggagat aacaataagc tcataaccaa
     1321 tcgacgtcat tggctgccca ctagtcatac gtgaagccag aataattccg gctatcgcag
     1381 ataccaggcc agagagaaca aagataataa ttttggtgcg aacaaccggt acacccgcca
     1441 gacgcgcagc ctcttcgttc ccgccaatcg ccagggtatt acgaccaaag gtggttttat
     1501 tcagcagcaa accaaagata atcagacacg cgacggtgag ccagattggc gcaggcagac
     1561 cgaaccagtt agcgtaacca agggcaaaga agctttcatc ttcgataccg accgctttac
     1621 cgtctgaaat gatgtacgcc agacctcgaa caatctgcat cgttgccagt gtcgtgatca
//
LOCUS       DAJTUP010000250         1677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTUP010000250 DAJTUP010000000
VERSION     DAJTUP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.257"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>1677
                     /locus_tag="OO468_004126"
     CDS             <1..>1677
                     /locus_tag="OO468_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR6261008.1"
                     /translation="SQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAA"
BASE COUNT          484 a          406 c          399 g          388 t
ORIGIN      
        1 cctctcaggc gcgccgccct ttcctgctgg ctaatacccg taatttagac cgtgaaccta
       61 tcggcctgat ggatactgtc gccacggtca cgccggcaca attgcgccaa ttttatcaac
      121 gctggtatca accaaataat atgaccttta tcgtggtcgg cgatatcgac agtaaagaag
      181 cgctggcgct gataaaggat aatttaagta agcttccggc taacaaagca gctgaaaatc
      241 gcgtctggcc gacaaaagcc gaaaaccacc tgcgctttaa tatcattaat gataaagaaa
      301 accgggtgaa cggcatcgca ctctattatc gcctgccaat ggtacaagtg aacgatgagc
      361 aaagctttgt cgaacaagct gaatggagca tgttagttca gctgttcaat caacgtctgc
      421 aggaacgcat acagtcgggc gagttgaaga ctatttctgg cggcactgcg cgcagcgtta
      481 aaattgcacc cgattatcag tcgctgtttt tccgtgtaaa tgcacgagac gataatatgc
      541 aggatgctgc gaatgcatta atggcagagt tggcaaccat tgatcagcat ggtttttctg
      601 ctgaagaact cgatgatgtc aaatctaccc gcctcacctg gctgaaaaat gcggttgatc
      661 agcaagctga acgtgattta cgtatgctga ccagtcgcct ggcatccagc tcattaaata
      721 atacgccgtt cttgtcgccg gaagagacat atcaactttc gaaacgtctg tggcagcaaa
      781 ttaccgtgca aagtctggcg gaaaaatggc agcagttaag aaagaaccag gacgcatttt
      841 gggagcaaat ggtaaacaat gagcttgccg ccaaaaaagc attgtctcct gcggctatcc
      901 tggcgctgga aaaagagtac gccaacaaaa agctggcggc ttacatcttc ccaggcagaa
      961 atttatcgtt aacagtagac gctgacccac aggcggaaat tagcagcaaa gaaacgctgg
     1021 ctgagaatct gacatcatta acactttcca atggtgccaa ggttattctg gcaaaatccg
     1081 cgggtgaaga gcaaaagcta caaattattg ccgtatcgaa taaaggcgat ttaagtttcc
     1141 ctgcgcagca aaaatcactt atcgcgctgg caaataaagc agttagcgga agcggcgttg
     1201 gcgaactctc ctcttccagc ctgaaacgct ggagtgcgga aaactcggta accatgagca
     1261 gtaaagtcag tggcatgaat acgttgctct ccgttagcgc gcggactaat aaccctgaac
     1321 ctggtttcca gttgattaac cagcgaatca cccacagcac gattaacgac aatatttggg
     1381 catcgctaca aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga
     1441 aattcgccca gctgatgtat gagacgcgct atgctgatga ccgcacgaaa ttactgcaag
     1501 aaaatcagat tgcacagttt actgccgcag atgcgctggc tgccgatcgc cagttgtttt
     1561 catctccagc ggatatcacg tttgtcattg tcggtaatgt cgcagaagac aaactcgtgg
     1621 cgttaattac gcgttactta ggctcaatca aacactctga ttcgccatta gccgcag
//
LOCUS       DAJTUP010000251         1644 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTUP010000251 DAJTUP010000000
VERSION     DAJTUP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.258"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(860..1196)
                     /locus_tag="OO468_004127"
                     /pseudo
     CDS             complement(860..1196)
                     /locus_tag="OO468_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          517 a          329 c          334 g          464 t
ORIGIN      
        1 taatggcaaa aaaatcaggt ctgataagaa acacttctta gaatgcaatg aagacaaata
       61 acaacaatct gcatattgcc accaaccaca gatgtggcat cgttatcagc gcacttgatt
      121 ttctttatat tcactgacga gcttgccggg gcacctttgt ttgccggtca ttctggtatc
      181 atgcagatat ttacggcgta caagcccact agcatgacat tgtaataagg cctgagccta
      241 acgctttaat gtgtgtcata cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat
      301 cgtaagcgtc cagcgaaatc catctggtca taatctgaag catccgacaa agtggtgtcc
      361 accaaataag tgatctgcgc ccaatatcct gagccggaac tttgacaaag gccaacgaaa
      421 aatgggagcg gatgttacag agttctcggt acaggggaaa agtggtacct gtcgccagtt
      481 ctccgatctg tttaaccggg aaattatctc ctacagcttg tcagaaaggt cggtgatgga
      541 gcatcgttaa tacgatgcta tacgatgcat tcacagtgcc cggccagagg atgccccgtc
      601 gctgcatatg gatcagggtt ggcaatatcg aatcgcaggc tataggcaaa gttaaagccc
      661 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatgaag
      721 aacttcttca gcacactgct aaaacgcata gtgatcgagc gctgattctg gcgaacaact
      781 gaactaatac atcagaatct gcattatgtt aaataaatat gaaaagatgg tttaaatacc
      841 ccgttacttt tgacttacac tatacggtat cgcatcgttt aatattcgca ccggccagat
      901 ttttatttct attagttgtc acaatactga atgcgtacga ccacagtatt ctggctcctg
      961 tgtggttatg ctttaattct gcgttccggg cagataagca gttgcttgca ggaatccttc
     1021 ttgtgttaat gtcagttccc ctttttacca gtgctgattt ccacattccg tccaacagag
     1081 cttatagcct ttccctggat tatagcattg tccggctgaa gttctttttg aataataata
     1141 gaagcactgc tggcagatcc agtccgtttt tcataaccca ctgtactgat aaccataatc
     1201 taatcagtag aaattgagtc gaaaacaagc actactctat acaggataat tagaggtcag
     1261 tttgattatt cacaattcat catcagcatt ttctatttct gacgaaatca acatgaaaat
     1321 aaccatatat gataattatt ataataacgg ctttaattgg aatacatata ttacaacgta
     1381 ttatatataa ttggtattct gggaactata ttctcaaaat acagtagaaa cgaggtatgt
     1441 ttctggtgga aaggacagtg ggattaaaaa gtaagaattg ataaaaaaaa cgccagcaac
     1501 acatagtgcc ggcggaggga ataccccatg gagaaaatgt gatgcctaga gcatcatgaa
     1561 tatataatta aaaatagtta gcgttgtcaa tactttcaca aaaataattt tcgtagtata
     1621 gaaagatatt tttatgcatg acct
//
LOCUS       DAJTUP010000252         1600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTUP010000252 DAJTUP010000000
VERSION     DAJTUP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.259"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..360
                     /locus_tag="OO468_004128"
     CDS             <1..360
                     /locus_tag="OO468_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6261009.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO468_004129"
     CDS             361..726
                     /locus_tag="OO468_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR6261010.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO468_004130"
     CDS             723..1406
                     /locus_tag="OO468_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6261011.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          452 a          324 c          438 g          386 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc
//
LOCUS       DAJTUP010000253         1577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTUP010000253 DAJTUP010000000
VERSION     DAJTUP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.260"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(5..184)
                     /locus_tag="OO468_004131"
                     /pseudo
     CDS             complement(5..184)
                     /locus_tag="OO468_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            708..992
                     /locus_tag="OO468_004132"
     CDS             708..992
                     /locus_tag="OO468_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR6261012.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            992..1267
                     /locus_tag="OO468_004133"
     CDS             992..1267
                     /locus_tag="OO468_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR6261013.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(1493..>1577)
                     /locus_tag="OO468_004134"
     CDS             complement(1493..>1577)
                     /locus_tag="OO468_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6261014.1"
                     /translation="RKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          427 a          393 c          384 g          373 t
ORIGIN      
        1 aaaattactc tttttcgaac tccagtgagc gttcacgact catcagtgtg aaaatgatgg
       61 caaaaacagc agacagtatc agtacgataa atgtggcccc ccatcccaga taatcgacaa
      121 caagtcccat tcccagtgtc gcaacaattg agccgaagat ataggaaaat gtgcctgaca
      181 gccctaacag aataagttta cgcgtaaatc gccctgaatc cagaagagca ttaagtatca
      241 cataaacagg ttccatacca gtcctgaccg catcaggacg ccagtaaata aaatctcgta
      301 gtccaccggc atcagagaca agagtaatcg tcgttaccgg tatttcgact ggtttgtact
      361 ggtgtgagcg gacaaaacta ctgtacgtct ctcatacacg ctcgtaagcg gccctcacgg
      421 cccgctaacg cggagatacg ccccgactgc gggtaaaccc ttgtcgggac cactccgacc
      481 gcgcacagag gcattttcat cgctgacagg ccgcatggcc tcgcagagcc ggagggggac
      541 tggtgatatt cggacgaccg taccggcttg cgccgggctt cggcggtttt acacctgtgc
      601 tacatgtaac caccgtatgc cgccttatat gcgctgactc gcattatttg ccttgttccg
      661 tctgaaagta atcactatga ttaaatatga ttaacaacta atcgggtatg caaatgaaaa
      721 acaataccgc acaagcaaca aaagtaatta ccgcgcatgt gccattacct atggctgata
      781 aagtcgacca gatggccgcc agactggaac gctcccgggg ctgggttatc aaacaggcgc
      841 tttctgcatg gcttgcccaa gaggaggagc gtaatcgcct gacgctggaa gccctggacg
      901 atgtgacatc cggacaggtt atcgaccatc aggctgtaca gtcctgggcg gacagcctca
      961 gtactgacca tctgttaccg gtgccacgct gatggaactg aagtggacca gtaaggcact
     1021 ttctgatttg gcgcggttat atgattttct ggtgctggcc agtaaacctg ctgccgccag
     1081 aacggtgcag tccctgacac aggcgccggt cattctgtta actcatccac gtatgggaga
     1141 acagttgttt cagtttgaac ccagggaggt cagacggatt tttactggcg agtacgaaat
     1201 ccgttacgaa cttactggcc agactattta tgtattgcgc ctgtggcaca cacgagagaa
     1261 caggtagatt gggtgtggag gcaactttaa catagcctgc aggtattaat tctggagcat
     1321 tgttatcagg ccagatattt tgcattccat taaataatct tgtaggagat aaaataaatt
     1381 acgatgaaat aacgaaaatt accgctcgtg actggcggca atatcgcgct ccgggctggc
     1441 aaataaccca tcagaaacgt tattgccaga cattgcgggt aatgactcca acttactgat
     1501 agtgttttat gttcagataa tgcccgatga ccttgtcatg cagctccacc gattttgaga
     1561 acgacagtga cttccgt
//
LOCUS       DAJTUP010000254         1577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTUP010000254 DAJTUP010000000
VERSION     DAJTUP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.261"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..189
                     /locus_tag="OO468_004135"
     CDS             <1..189
                     /locus_tag="OO468_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6261015.1"
                     /translation="ENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLT
                     DEVLALRLSENGSQLHHS"
     gene            397..666
                     /locus_tag="OO468_004136"
                     /pseudo
     CDS             397..666
                     /locus_tag="OO468_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            663..889
                     /locus_tag="OO468_004137"
                     /pseudo
     CDS             663..889
                     /locus_tag="OO468_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            918..1508
                     /gene="ospG"
                     /locus_tag="OO468_004138"
     CDS             918..1508
                     /gene="ospG"
                     /locus_tag="OO468_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR6261016.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          449 a          314 c          398 g          416 t
ORIGIN      
        1 gagaatgagt accctcagag ggtggctgac cggctgaaag catcaggtct gagcggtgat
       61 gcggatgcgg agagggaagc cggtgcacag gtgatgcgtg agactgaaca gcagatttac
      121 cgtcagctga ctgacgaggt actggccctg cgattgtctg aaaacggctc acaactgcac
      181 cattcataat cacatcgcat aaaactgtca gcgccagtga taggacaggt gaaacctgtc
      241 aatacgtacc ggcttacgcc gggcttcggc ggttttactc ctctgccata tgcaacaacg
      301 gagtgccgcc ttacatgccg ctggcgcggc atatcctggt gacgatatct gaacagttat
      361 atactgtgta tatacgtggt aatgacgagg tggcaaatgg cacaggttaa tatgagtgta
      421 agaatcgacg cagaactgaa ggatgctttt atggccgctg caaaaagcat ggaccgtaat
      481 ggctctcagt taatccggga ttttatgcgc cagaccgttg aacggcagca caattcttgg
      541 ttccgtgact aggttgcggc aggccgtcag caactcgaat gcggtgatgt gcttccccat
      601 gacatggtcg aatcctctgc tgctgcatgg cgggatgaaa tgagcaggaa gattgctgat
      661 aaatgatgga gatgttctgg accatgctgg ccatccagga caggaagcgc attcgtagta
      721 cattgcggag cagaacctga tggccgccat tgaaccggac gaacggattg gttattcagc
      781 cagcagcctg gcagggcagc cgtataaagg ccgcaacggt cgtgtggaag gaacatctgg
      841 accacacaag gtagcgtgta acgttatttt gtgtgaaaat ctcttgtgat ttttaaatat
      901 cgtaacaaag gtatgacatg aaaataacat ctaccattat tcaaacacct tttccatttg
      961 agaataataa ttctcatgct ggcatagtaa cggagcccat tctcggtaag ttaataggtc
     1021 aggggtcgac agcagaaatc tttgaagatg tgaatgattc atctgctttg tataaaaagt
     1081 atgatcttat tggcaaccag tacaatgaga ttctggaaat ggcttggcaa gaatctgagc
     1141 tttttaatgc tttttatggc gatgaagcat ccgttgttat acagtatggc ggagatgtgt
     1201 acctccgaat gctgcgcgtg cctgggactc cccttagtga cattgataca gctgatatcc
     1261 ctgataatat agagagcctt tatctacagt tgatatgtaa attgaatgag ttgagtataa
     1321 tccattacga tcttaataca ggtaatatgc tgtatgataa agaaagtgaa agtttattcc
     1381 caatagattt tcgcaatatt tatgctgaat attacgctgc aaccaaaaaa gataaagaga
     1441 ttatcgaccg acgattacaa atgcgtacaa atgattttta ttcgttatta aacaggaaat
     1501 atttatagac gtatttgttg atgctataag ccagaggctc ttctatgaga atatggggac
     1561 cattccctcg cgatatt
//
LOCUS       DAJTUP010000255         1567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTUP010000255 DAJTUP010000000
VERSION     DAJTUP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.262"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..496
                     /locus_tag="OO468_004139"
     CDS             <1..496
                     /locus_tag="OO468_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR6261017.1"
                     /translation="LHASTNAPGSDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQK
                     THYSDTRWHEDSVRNRWFSVMAGLSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDN
                     KGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGV
                     GYKF"
     gene            560..>1567
                     /locus_tag="OO468_004140"
     CDS             560..>1567
                     /locus_tag="OO468_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6261018.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAG
                     EKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSD
                     FLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGI
                     NG"
BASE COUNT          373 a          381 c          493 g          320 t
ORIGIN      
        1 tcttcatgcc tcgacgaacg ctcccggcag tgatgatctg aacgggatta acgtgaaata
       61 ccgttatgag tttacggaca cgctggggct ggtgacgtca ttcagctatg ccaacgctga
      121 agatgagcaa aaaacgcatt acagtgatac ccgctggcat gaggattccg tgcgtaaccg
      181 ctggttcagc gtgatggcgg ggctgtctgt gcgcgtgaat gaatggttca gcgcgtatgc
      241 gatggcgggc gtggcttaca gccgtgtgtc gaccttctcc ggggattatc tccgcgtaac
      301 tgacaacaag gggaaaacgc acgatgtgct gaccgggagt gatgacaacc gccacagcaa
      361 cacgtctctg gcgtgggggg ctggcgtgca gtttaacccg accgaatccg tggccattga
      421 ccttgcttat gaaggttccg gcagtggcga ctggcgcact gacggtttca tcgtgggtgt
      481 cggttataag ttctgattag ccaggtaaca cagtgttatg acagcccgct ggttcaggcg
      541 ggctttttgt ggggtgaata tggcagtaaa gatttcaggt gtactgaaag atggggcggg
      601 taaacctgtc gtaaattgtg cgattgaact gcgggccaga agaaccagtc cgaccgttgt
      661 ggcacacgtt gttgccactt gcgtgacgga caataacggt gcttatgtga ttgaggctga
      721 gccggggtat tacgaggttg cgcttcactg taacggctgg cagccaaccc gtgtcgggga
      781 tattgatgtg gcaccgactg atgcaccggg gacactgaac gcgtttctga atgcaccaaa
      841 ggatggtgat ttacgtccgg aggtgatgaa gcgctttgag gaaatggtgg cgcaggcgca
      901 gcagagtgcc ggggctgcag ccggaaacgc acagcagacg gcgcaggatg tggcggcagc
      961 cgcaacggcc cgtgatgatg cacaacgttt tgcggagaaa gcccgacagg atgcaaccgt
     1021 cacagctgag gacagaaagg ccactgcgga agatgtgaca agcacaggag caaatgcagc
     1081 cgcagccgga cagagcgcac aggatgccgc aggttatgcc cgcgcagcag aacaggccaa
     1141 aaatgacatt gatgctgcgc tgaccggcac tctgaaaatg gctaaccatc tgtcagaaat
     1201 cgcagcagca ggcgaaaagg cacaacagaa gtcccgggat aatctggggc tgaaaagtgc
     1261 ggccacgatg gaagcacaga gcgacattta cgaccggaca aaaggccgtc tggcgatacc
     1321 cggcgcattc ggctttgggt gtgcttttct gcctgaagat gttatccgtt ttgacactaa
     1381 gagtgatttc ctggcctggg taaggaatgc gctgccaggt gaatattccg ttgctggccc
     1441 ctacggcatc atcatacccg acacacggtt tgaaggggtg ctcagcatcc ggtggactga
     1501 tgcacgccct gagacaacag aaccgcggta cagagccaaa tcccttactt tttacggcat
     1561 taacggc
//
LOCUS       DAJTUP010000256         1553 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTUP010000256 DAJTUP010000000
VERSION     DAJTUP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.263"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..327)
                     /locus_tag="OO468_004141"
                     /pseudo
     CDS             complement(1..327)
                     /locus_tag="OO468_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            756..1544
                     /gene="virF"
                     /locus_tag="OO468_004142"
     CDS             756..1544
                     /gene="virF"
                     /locus_tag="OO468_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR6261019.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          491 a          284 c          283 g          495 t
ORIGIN      
        1 attgtaatgc tcgatcgcca ctgcaaggtt atgcaatgcc gttcttggtt tcggcatgaa
       61 cgcgatgcag tcttccttca tcgttttcat gaaccgttct gctattccat tactctgagg
      121 gctactgact gccgtcgtgc agggctccag attcaactct ctggcgaact gccgtgtttc
      181 atgagccgta taagccgaac cgttatcggc cactggattg gccgttccgg tcctttatcg
      241 ccatatcgct cctgacatga catcctgcac cgttgctttg tcataatctc ccgtgcctgc
      301 tgcccagtct atggcttcac gatcgcaata tggaacgtaa cgctgtcatc gggagaatcg
      361 tcaatgcggc attcggttaa cgcttaccaa tatcaacacc aaccggattt gtggagcctc
      421 cagtctgaag gggctttatg cgttccgtat aggatattat gatgctggag ttttgcgaag
      481 ctccactcct ttccgaacat ttacacagat attgcctaag aaaagtgaaa ccataatatg
      541 gttatagtcc ctttcagtgc aaatacttag cttgttgcac agagaaatag aagctgcata
      601 agctctttct tcaaaaaatg taaataaagt taaatatagg aaaaattact taatctatct
      661 taataacgga aagattttgt atacaatcac tgttacagaa atttcttagt tactctgtaa
      721 acactaaata tagtttggta tattctgttg aatttatgat ggatatggga cataaaaaca
      781 aaatagatat aaaggttcgc ttgcataact atattatttt atatgcaaaa aggtgttcaa
      841 tgacggttag ctcaggcaat gaaactttga ctatcgatga agggcaaatt gcttttatag
      901 agcgaaatat acaaataaac gtctccataa aaaaatctga tagcattaat ccatttgaga
      961 ttataagcct tgacagaaat ttattattaa gcattattag aataatggaa ccaatttatt
     1021 catttcaaca ctcctattct gaggagaaaa gggggttaaa caaaaaaata ttcctcctct
     1081 ctgaggagga ggtttctatc gatttgttca aatctataaa agagatgcct ttcggcaaaa
     1141 gaaagatcta tagtttagct tgccttttat cagctgtttc tgatgaggaa gctttatata
     1201 cttcgatatc gatagcttct tctcttagtt tttctgatca gataaggaag attgttgaaa
     1261 aaaacatcga gaagagatgg cgtctttctg atatttcaaa taacttgaat ttatcagaaa
     1321 tagctgttag aaaacgattg gagagtgaaa aattaacatt tcaacaaatc cttcttgata
     1381 ttcgcatgca tcatgcagca aagcttttat tgaatagtca aagctatatt aatgatgtat
     1441 caagacttat cggaatatca agcccatctt attttataag gaaatttaat gaatattatg
     1501 gtataactcc aaagaaattt tacttatatc ataaaaaatt ttaaatgctt cat
//
LOCUS       DAJTUP010000257         1550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTUP010000257 DAJTUP010000000
VERSION     DAJTUP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.264"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1156)
                     /gene="arsB"
                     /locus_tag="OO468_004143"
     CDS             complement(<1..1156)
                     /gene="arsB"
                     /locus_tag="OO468_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR6261020.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLL"
     gene            complement(1210..>1550)
                     /gene="arsR"
                     /locus_tag="OO468_004144"
     CDS             complement(1210..>1550)
                     /gene="arsR"
                     /locus_tag="OO468_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR6261021.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT          426 a          436 c          389 g          299 t
ORIGIN      
        1 agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg cagccaatca
       61 cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca tcaatggaca
      121 acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag gcggtgagga
      181 atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg agtacgccag
      241 aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac atgccgagcg
      301 agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta atcgcatgac
      361 cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg ctcaccggaa
      421 tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca acccagccag
      481 ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc gccatatcgt
      541 agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg gcaacaatcg
      601 cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg ccaaagaaat
      661 cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg gtatcggcaa
      721 tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct aaagccagca
      781 gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca aacagggccg
      841 caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt ccattgcccc
      901 agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc agcaggctga
      961 tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac accaccggaa
     1021 tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc gttgcactcc
     1081 agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg acaaagatag
     1141 cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca tatgtgttta
     1201 gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc tggccaggtt
     1261 gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa taattttcgc
     1321 cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt gcttgcggtc
     1381 cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg gctgcgactg
     1441 atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa gcagtaaaac
     1501 gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
//
LOCUS       DAJTUP010000258         1549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTUP010000258 DAJTUP010000000
VERSION     DAJTUP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.265"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            351..476
                     /locus_tag="OO468_004145"
     CDS             351..476
                     /locus_tag="OO468_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCR6261022.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
     gene            complement(921..1397)
                     /locus_tag="OO468_004146"
     CDS             complement(921..1397)
                     /locus_tag="OO468_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6261023.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          361 a          406 c          382 g          400 t
ORIGIN      
        1 aaaaaaacaa ccacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgtg
       61 caacgcaacg ccctttgggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctatgcaag cctcgccagc cgtgattttt gccagatttc
      181 gtggttttca cgttcaacag ctccatcgtg tttttgccgt ccgttcaccg ttaaaacgcc
      241 tcagaaatcg ggacaggatg tgtaatcgta taaccgcgca tgcacgccga tacgattacc
      301 acttggtcag ggcttcaccc cgacaccccg catcagtgcg taagtgctca atgtggcttc
      361 cagttctctt ccgcttcctc gtcgattcgc tcgctacgct cggtcatcga cttgcggcga
      421 gcgggatcac ctctcgcatg ttgtcggtga tcccggtgtt gttcttttgg ggatgagtcg
      481 ccagatagcc cctcaaacgc gctcaaaagc tctcagacgc acgaaacggg ttttagatgc
      541 ccgccagggc atggaaggcg cgaaaaacgc ttacagaggc tctcaggagg tcaagcgcgc
      601 cgatacccct aaccaggggc tttcaggtcg ccaggaaccg cgccagcccc ctgcaaagcc
      661 catttgcacg gaacaggcag ccatgcggtt ggtaggtggt cgtagaccgt cggtgttgca
      721 gcgatggggg tctggggttc tccccagacg gtttccggta gcgctcaagg cgctgccgga
      781 accgtggcgg ctcggttttt tctggagaac ggggagcaaa aacacgcaaa ggattttgag
      841 gcggcaaaag agcaatgcag gatagacaat catggtcaga acgttaacac gtagcccctg
      901 attgtccgcc tgccgctagc tcaatttgaa aacatggcca tgattcggcg ctgaatatca
      961 ccgtctttgt tgaccagtgt tttagtgaca aacgctgcat ggtttttcgt agtgttgtat
     1021 cccttccaga cttcatgtgg atcgagggca tacgcacaaa ccttaccagc aacggggatt
     1081 ctaactaatg cattttttga ttctaagtaa ttgagccacc gaataattgt gcttctgcta
     1141 actccaagtt tttcagctag aaattgctga tccgcaacga cagcgccaca cgtaggatcg
     1201 atatgctccg ctaaaaatga gtacagcttc agtgctgata tgccttggct atccttcagc
     1261 agctctcgaa cacgttccca accttttggg gatacctgtg taaaccttga atttttcttt
     1321 gcttcttcga gatcctcggt gtttttcttt gcctcatctg aaagttgttt cagtcgtttt
     1381 tctgaatcaa taaacatgat tttttccctc tgaaaatccc atgcactaac gttggttcaa
     1441 atctaagcca tcatgacaca tctatgcaac accttgttgc gtgttgtcgc atcctggatg
     1501 cgacaacgtt gtgtcatcca ggatgcgaca ggtaaataaa aaacacact
//
LOCUS       DAJTUP010000259         1545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTUP010000259 DAJTUP010000000
VERSION     DAJTUP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.266"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>1545)
                     /locus_tag="OO468_004147"
     CDS             complement(<1..>1545)
                     /locus_tag="OO468_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6261024.1"
                     /translation="SQNGFAPVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLN
                     TASTGGDLDVTIKEEDGSEQRFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVL
                     QAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTE
                     TGQSYRFLYSKLFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRV
                     EGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTAR
                     QGTGNNHASDNTSEQIYMLSLSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALE
                     RNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAH
                     SEGVTLSQELGETIALVKAPGAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISD
                     NYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQ
                     DASLADS"
BASE COUNT          397 a          403 c          338 g          407 t
ORIGIN      
        1 tgaatctgcc aaacttgcat cttgtatcgc agcaatggct ccaaaaggta ccggtttacc
       61 atttgcattt aacactctga agagaacgcg ataacccaca tgagtcacaa actccgcttt
      121 aaccactgca ccacgcgtgg gaaccatcgt aacgacggta ttatcaagtt ctatattcga
      181 cttcgagaaa tagttatcgc tgattgctac ccgattttca tcataagggt ttagctgtgt
      241 cttgaccgta tagccgcgcc agtccgtcgc agcaccgcgc atattatcta tttctaacct
      301 ggcggcccca ggtgctttaa caagagcaat agtttcacct aactcctgac ttaacgttac
      361 cccttcgctg tgtgccagaa ttccccctct gataccataa ttcagcctct gggaattttg
      421 gctgtagtta tagctgccgt tcaccgtgcc ataagagcca tcataggtca gggacatatt
      481 accgccaacc tcatcatcat gactgttata ggactgcatt aaattccatg aaaggttatt
      541 tctttccagc gccgtaccgc tgagtccgag ttgatgtgag ctaccggaat tatcgttttg
      601 cgaaacagaa taggtggcgt aactattacc ccaccagccc gataaaggaa cagataaaga
      661 gagcatgtag atctgctcac tggtgttatc actggcatgg ttgttccccg tcccctgtct
      721 ggcagtattg gaataactcc aggagacgtt ccacgagaga cgcttccagc tactgctgta
      781 gccaaattgc attaatcgtt cgacatcatc ggtatgccag tattgttgcc ggcttaatgt
      841 caggtataaa ttgccataat ctctccccaa cgactgcgtt agcgttccct ccacgcgact
      901 acgtcggtta cctgtttccc aaaagtcttc agggctgttg cgccgcgatg cccactcatt
      961 gagggtatag tagccctcgg tggagtaacg atagccaacc aggcgcaagc tagtgtctgt
     1021 gtcgtcaaat agttttgaat agagaaagcg atatgactga cccgtttctg tatcatcatg
     1081 gctaaaattc gcacgagcat gtgtcacatc gaaagaaatt gcgcccaaag cgccaagatc
     1141 tttaccgaca cccagagctg cagaaccata attttcagca gcctgcatac ctccatataa
     1201 agtgatcccg tgggaaaaac catgaagtat ttgcgcctga aggacgtccg gtgtaaatcc
     1261 gtcttcatcg cgcaattcac ccacgctgac atcaacatct gtcagacctt cacgtttaag
     1321 aatcgccaat gaagcataag gttgggtgaa tcgttgttcg ctaccgtctt cttctttgat
     1381 ggttacgtcc aaatcgccac ctgtacttgc ggtgttgaga tcggtgatct caaaagcacc
     1441 ttgtggaacg gctgactgat aaatcacgta gccattctgc cgaatgatga cggtggcgtt
     1501 ggactttgca atcccacgaa ccacaggagc aaagccattc tggct
//
LOCUS       DAJTUP010000260         1525 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTUP010000260 DAJTUP010000000
VERSION     DAJTUP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.267"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..284
                     /locus_tag="OO468_004148"
                     /pseudo
     CDS             1..284
                     /locus_tag="OO468_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            286..652
                     /locus_tag="OO468_004149"
                     /pseudo
     CDS             286..652
                     /locus_tag="OO468_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            580..1053
                     /locus_tag="OO468_004150"
                     /pseudo
     CDS             580..1053
                     /locus_tag="OO468_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
BASE COUNT          377 a          378 c          460 g          310 t
ORIGIN      
        1 attaccactg tcagcaacag cgacatcatc atgataaacg cagtgcccgt gcgcagcgcg
       61 atgactggct gaagaaagag atactgcgcg tatacgatga gaatcatcag gtatacgctg
      121 tgcgtaaagt ctggcatcag ttgttacggg aaggtatcag agtggccaga tgcactgtgg
      181 cacgtctcat ggcggttatg gggcttgccg gtgttctccg gggtaaaaag gtccacacta
      241 ccgtcagccg gaaagccgtt gccgcaggcg accgcgtaaa ccgtcatcaa ggcaatgtgc
      301 cgcgtactcc gggtggcccg cagcggctgg tatacgtggt gtcagcggcg gacaaggata
      361 aacacacgtc cgcagttccg tcagcactgc gacagcgttg tccgcaaggc ttttacccag
      421 tccagcgtta cggtgcccca cgcctgacgg atgaactgtg tgctctggtt accactttaa
      481 cgtaaaaacc gtggcagcca gtctgcgccg tcaggaactg agcgcaaaag cctcccagaa
      541 gttcagcccg atcagttacc gtgcacacgg cctgcctgta tcagaaaatc tgctgacgca
      601 ggatttttac gccagtggcc cgaaccagaa gtgggcggga gacatcacgt actattacag
      661 ctcaccgact gcggggaaac acggggcgcc gggttattga aggtgacctg tccagctact
      721 tcgacacagt acatcaccgg ctgctgatga aagccgtctg ccgcaggatc agtgatacac
      781 gttttatgag actgctgtgg aaaacgccgt gttgatgttg gtctcttccg ggcggtcagt
      841 gaaagtatgc cacagggcgg tgtgatctcg ccgttgctgt cgaacatcat actgaatgag
      901 ttcgaccagt atctgaataa acgctacctg agtggaaaag ccagaaagga tcgctggtac
      961 tggaatcaca gtatccagcg aggccgaagt actgctgtca aagaaaactg gcaatggaaa
     1021 cctgccgttg cttactgttg ctatgccgat gactgtgtgc cacgaaggcg cgtcctgggg
     1081 acgtgagtcc ctgtgcagcc atgcagcaca agagatgagg gaaggcccct tggtgtcggc
     1141 gtgcaggcgc aggcaccaaa ccatccactg ctgctgcatt taagagatgt ggtggtgagc
     1201 gagatgggaa aaggtcggac gtgagccgat caggtatggt tcgaagagac gaacgaaagt
     1261 gaaccgctga tgatgtgtcg aaagcatata gatgatgtca aaacaatggt ggagtcgttc
     1321 ctgtgggata tgtttactgc ctgcgcggca tccggcataa aagcggcgtg aagtcgattc
     1381 aggctcttgt ggggaacgtg gacctgtcac cccgatgtga agggagaagc gcaagtggaa
     1441 gccccatgag cgccagagta ccaatgcggg gtacagggac ggagcaccaa gcgattgcgc
     1501 agtaaacatg cttgacaact taatt
//
LOCUS       DAJTUP010000261         1522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTUP010000261 DAJTUP010000000
VERSION     DAJTUP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.268"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..247)
                     /locus_tag="OO468_004151"
     CDS             complement(<1..247)
                     /locus_tag="OO468_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR6261025.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTA"
     gene            complement(244..>1522)
                     /gene="btsS"
                     /locus_tag="OO468_004152"
     CDS             complement(244..>1522)
                     /gene="btsS"
                     /locus_tag="OO468_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR6261026.1"
                     /translation="VFNPITAGAVTFVAEMVQMLIILAIARPYEDAVRLVSNIAAPMM
                     VTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAASTEGILRQGFNEVNSMKVAQV
                     LYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSTYTLKAIETGEVVYADGNEVP
                     YRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNRLFSSINRTLGEGIAQLLSAQ
                     ILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIKAVIRRDSEQASQLVQYLSTF
                     FRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQVNIAIPQELSQQQLPAFTLQP
                     IVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLYQPVTNASGLGMNLVDKR
                     LRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          372 a          410 c          358 g          382 t
ORIGIN      
        1 acgcagtgag aaaaacaata tacgggcgat gttccgggtc aagcatcccc accatttcca
       61 gaccactgat gcgcggcatc tggatatcga gaaacagcac atccgggcgc agtttatgca
      121 ccgcgccgat cccttccacg gcgtttgaac actctccaac gatttcaata tcgctctgct
      181 cctgcaaaaa tatacgcagg ttctcccgtg ctaacggttc atcatcgaca attaagactt
      241 taatcatgcc tcgtccctcc atggtagtcg taacgttatt cgggtgtaac tatcaggctc
      301 acaggcgacg cttattccat agtcatcgcc aaaccgttca cgtaaacgct tatccaccag
      361 attcatcccc agcccactgg cattggttac cggttgatac aaaccggcat tgtcttcgat
      421 ctccagcatc aaatgttgcc cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag
      481 cagttgtgat gtcccatgtt taatggcgtt ttccactatc ggttgcaggg taaacgcggg
      541 caattgctgc tgggataatt cttgcggaat agcaatgttg acctgcaacc gcgactggaa
      601 gcgcgccttt tcaatttgca gataagcgtt cacatgttca atttcgtcgg cgagagtaac
      661 aaactccgaa ggccgcttta agtttttgcg gaaaaaagtg gaaagatact gcaccagctg
      721 gctggcctgt tcgctgtcgc ggcggatcac cgctttaatg gtgttaagcg cattaaacaa
      781 aaaatgagga ttcacctggg cgtgaagcag tttgatttct gactgggtga gcatcgcttt
      841 ttgccgctca tattgccctg caagaatctg cgccgaaagc agttgcgcaa tcccctcgcc
      901 cagcgtgcgg tttattgaac tgaataaccg gtttttggct tcatacaatt tgatggtgcc
      961 catcacgcgc tgattttcgc cacgcaacgg aattaccagc gtcgacccca gtttgcattg
     1021 cggatgcaaa gagcaacgat aaggtacttc gttgccatca gcgtagacca cttcaccggt
     1081 ttcaatcgct tttaaggtgt aagtcgaaga aatcggtttg ccgggtaaat ggtggtcgtc
     1141 accaattccg gtaaaggcca gcaatttctc tcgatcggta atcgcgactg caccaatatc
     1201 cagctcctga tacagtacct gagccacttt catgctgttc acttcgttaa acccctgtcg
     1261 caaaatgcct tccgtcgagg ctgccacttt cagcgcagtg gcagaaaaag ccgaagtgta
     1321 tttttcaaac atcgcgcgtt tatcgagcaa tatacgcata aacagcgccg cgccgacggt
     1381 attggtgacc atcattggcg cagcaatatt actcaccaga cgcaccgcat cttcataagg
     1441 tcgggcgatc gcaaggatga tcagcatttg caccatttca gcgacgaacg tgacggcacc
     1501 ggcggtaatg gggttaaaga ct
//
LOCUS       DAJTUP010000262         1510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTUP010000262 DAJTUP010000000
VERSION     DAJTUP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.269"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1126)
                     /gene="mppA"
                     /locus_tag="OO468_004153"
     CDS             complement(<1..1126)
                     /gene="mppA"
                     /locus_tag="OO468_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6261027.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGP"
BASE COUNT          412 a          373 c          327 g          398 t
ORIGIN      
        1 gcggaccata accagctgcg ctcaacaaag tttttgcctg cgcattcagt tcttcctgac
       61 tcatttgttc aaacggcgaa ggttccggcg taaatccggc ggtaacatct ggtataaaat
      121 gccacgctgg cttttcgccc gtccccaaca ctttttcggt catcaggcgg cgatctatcg
      181 tcatacttaa tgccaggcga acgcgctgat ctgccgtcgg ccctttttgc gtgttaaagg
      241 cataataata ggtcccgagc tgcggtggcg tataaacctg ccccggaata tccttcaaca
      301 gcttctgata catatttttc gggaaggatt cggtgatatc aatatccccc gcgaggtaac
      361 gcttagtggc tgcggattcc tgattaattg gcaggaaggt cactttttgc agcaccgttt
      421 tggcgttatc ccaataatgg gtattcggta ccacgaccag tttttcattg actacgcgct
      481 ctttaagaac ataagcgcca ttgccgatca gatttccggg tttcgtccac tctttaccgc
      541 tttctacgtt ggctttttgc accgggaaga aggcaaagtt agcggttaaa ttcacaaacc
      601 acggcaacgg tttatcaagc tgaattttca aagtatgggc atcaactgcg gtgacgccaa
      661 gctgatcagg cgtagcttta ccatcaataa tcgcctgtgc gttgttgatt cccgccagcg
      721 cggcaaacca tgcaaatggc gacaatgttt ttgggtccac cagacgttgc cagctgtaga
      781 caaaatcttg cgccgttacc ggtgtgccat ccgcccattt tgcgttatcg cgcagggtaa
      841 aagtccagat acggttgtca ttacttttcc attgagtcgc gacgccgggg acaatctccc
      901 ctttttcgtt ctgattcacc agaccttcaa acagatcgcg aatgacctga atctctggca
      961 ggcccacggc tttagcggga tccagcgacg caggctcatc tttaatgtgg cgcaccagct
     1021 cctgcttctc tgccagtact gtgccgctcg gaacttctgc agcatacgaa agagaaatgc
     1081 tgctgaccaa cagcgcacaa cacgtaactg aaacagagtg cttcataagt ttccctctaa
     1141 ccgacattaa aattgatgcg taattatttg tttcgcctta aagaaatgca aacaacttcc
     1201 atcaaaaaag tgacaaagga agcatcaaat gttttaagta tcaatatatc aaatcagatt
     1261 tagtgtaaat aatagacgcc aaaatgtggc aaagcacata gaacaccgaa cactacgttc
     1321 gggcttcgca cgttcaaacc agttttcggt atcataatca gcatctgtcg ccattgtacg
     1381 aagaataaaa aataaccaaa acgttgaaag gaactgagaa tacgttaaga gaagaattaa
     1441 attaatgtat cagcaagtag tgctaaacag cacctttata cttcagccta tctataaata
     1501 atgagaatgc
//
LOCUS       DAJTUP010000263         1489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTUP010000263 DAJTUP010000000
VERSION     DAJTUP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.270"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..598)
                     /locus_tag="OO468_004154"
     CDS             complement(<1..598)
                     /locus_tag="OO468_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6261028.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERV"
     gene            complement(595..957)
                     /locus_tag="OO468_004155"
     CDS             complement(595..957)
                     /locus_tag="OO468_004155"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HCR6261029.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(1307..>1489)
                     /locus_tag="OO468_004156"
     CDS             complement(1307..>1489)
                     /locus_tag="OO468_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6261030.1"
                     /translation="GVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDLILNPS
                     VPNLSQSRNKEGTNDV"
BASE COUNT          424 a          333 c          302 g          430 t
ORIGIN      
        1 caacacgctc tgtgcgtaca tattcaacga catctgtctg accaccaacc cagctcagta
       61 tgcctttcat gaaaagattg cgctcaggca atagtttaat gttctccaca acctcacgag
      121 acatgagtcg aaaatctccg acattttcct ctatttttgg ggtgcttatt ttgttgtgta
      181 atttatagaa ccactcagct gtcttacgtt tcagccgtcc atcagttgag cggtctgaac
      241 gtttagcaag caccatgtca gcacctgcct gccacttttc aataagatga gggataacct
      301 caatcgggtc ttgcaggtca acatcaattg gaatcacggc atcgccggtt gtatggtcta
      361 accctgcaaa caatgctggt tctttaccaa agttgcgtgt aaatgacagc ggaacaacta
      421 gcggatcaga aacagccaga gcgttaatga ttgactccgt agcgtctttg cttccgtcat
      481 ttatgaaaac aatttctact tcatatggct tcaactcttc gaattcacgt accgttttat
      541 aaaaaattgg tatcgcttct tcttcattga agacaggaac gacaagagat atcttcattt
      601 cgcatcccta aagacaatga actttgaata gacgaaaccg cacaccaagc tgatggcgga
      661 gaaggtaaca agagtgacaa ttggaggaag tgaacatcta tcagcaaccc atccaacagt
      721 agcactcagt gttcccatga acccgacata taacatgtag cgcatcgttg tagttgatgc
      781 tttgaatgtg aattttgcat tcgcaaagaa gctaaaactc acagccacaa cgaaacctgc
      841 gaagtttgcc atagcctgat tggtatgcgc ggcatagata catacaccaa aaaccaccca
      901 gtgtataagt gtgttcagca cgcctataga tgtgtacttt gcaaatagct ttaacatttc
      961 ttctatcagc taataatcaa aggcatgaag tctatcatcc aagtctcaat cgatcgatac
     1021 ttgcggtagt tgatgagaaa aactctggca cacaaatctt tgcactggat tgcaaggctt
     1081 tgtgctcttc tgtgaatgtg tggctatatg tttgaagatc gttgtgccgt atttgtgaca
     1141 tatatatggc aacatcattc atcaactttc tgtttgtgcc atcaactata tcttagtgaa
     1201 tgcggttaat gcttgctaaa acagatagtt atgattggtg ctacagattc gtaatgcgaa
     1261 ggtcgtaggt tcgactccta ttatcggcac catttaaatc aataagttac acatcattag
     1321 taccttcctt atttcttgac tgggacaaat ttgggaccga tgggttcaga atcaagtcta
     1381 tttgccgtgc gtgttcggta aggtgattag gtgcgaggtg agcatatcga cgaaccattt
     1441 cgatagactc ccagcctccc atttcctgta acactgataa cgggactcc
//
LOCUS       DAJTUP010000264         1486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTUP010000264 DAJTUP010000000
VERSION     DAJTUP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.271"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..862
                     /locus_tag="OO468_004157"
     CDS             <1..862
                     /locus_tag="OO468_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6261031.1"
                     /translation="QQLPRNRKQRFKDVPLADLLSREDKTDCLDVVTINNKYLIKMAA
                     VFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQLLVAAKAYSQKTSGKP
                     YHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINEDDSSLPGKSIKNAHSDR
                     CVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKNGYGEQVSKWFNRTLLP
                     KVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGSITYDRYAKEVEVDRLV
                     NVMADVYKET"
BASE COUNT          428 a          292 c          355 g          411 t
ORIGIN      
        1 ccagcagtta cctaggaacc gtaagcagcg cttcaaggat gtcccacttg ctgacctgtt
       61 gagccgtgaa gataagacag attgtctgga tgtcgttacc atcaacaaca agtatcttat
      121 taagatggct gctgtcttta ggtgggctgt gcgcaatgac ttgattaaaa agaacatgac
      181 tgagggtctg gagcttaaag tccctcagcg taaagcctca ggggcccgta acgccttctc
      241 tacggagcag gtggggcaac tactggtcgc agctaaggca tactctcaga agacctctgg
      301 taagccgtat cattattatg ttaccgcctt ggctgctatc actggggcga gactcaatga
      361 aatagcccaa ctacaggtca aagacgtcag gaccactgag gcgggaaccg tttatattca
      421 tatcaatgaa gacgatagta gtctacccgg taagagcatc aagaacgctc atagtgaccg
      481 ctgtgtgcct ctggtcgatg gggcctatgg ttttatattg gctgacttta tggcgcttgt
      541 agagacccgt agaggcgctg acggtgatga cgctatggtg ttcgatggtc tgcgtctcat
      601 gaagaacggt tacggtgagc aggtgagtaa atggtttaat cggacgttgt tgcctaaagt
      661 cctcgtagac cgtagcggct tagcgtttca ctcgtttaga catacagttg cggcccagtt
      721 aaaacaacat ggggtcgagt tggcgtatgc acaggcgatc atggggcata gttcagggtc
      781 tattacgtac gaccgttatg ctaaagaggt cgaggttgat aggctggtca atgtgatggc
      841 tgatgtttat aaggagacct agaggagacc atagtctagg atgacactta ctacagacct
      901 gtgtggtcat gtttttgact catagttttc tctacttgtc aagggataat taatacgact
      961 cactataggg agaagggaca caaaaagttt tctcttctag gatgacactt actacagacc
     1021 tgtgtggtca tgtttttgac tcatggtttt ctctacttgt caaggataat taatacgact
     1081 cactataggg agaagggaca caaaaagttt tctcttctag gacaactaac cgtaacttat
     1141 cgcatccttt caggggcggg atgaccaatt acagacacca agcgttctac ttaaagtgaa
     1201 tgcttcctgt gtgctctggg tcagtcgata agtgaatagc aataagacac aatcatctta
     1261 attatggtct ctagtcccta caggggctta acttaaagtt aacttaaaga cacaattaaa
     1321 gattaactta tagtattact taaagttaac ttaaagacac aattagagat taactttaag
     1381 tattaaataa aagatttaat taaagaccaa ttaaagatta actctaaggg aataccttaa
     1441 ggggtgatga tatttataat aatcaatcac caaacctata ggtctt
//
LOCUS       DAJTUP010000265         1455 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTUP010000265 DAJTUP010000000
VERSION     DAJTUP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.272"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..771)
                     /gene="ipaH4.5"
                     /locus_tag="OO468_004158"
     CDS             complement(<1..771)
                     /gene="ipaH4.5"
                     /locus_tag="OO468_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR6261032.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSSLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNP"
     gene            complement(1199..>1455)
                     /locus_tag="OO468_004159"
     CDS             complement(1199..>1455)
                     /locus_tag="OO468_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6261033.1"
                     /translation="AVLKRTEADRWAQAEEQKYEMLENEYSQRVADRLKASGLSGDAD
                     AEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSRLHHS"
BASE COUNT          387 a          304 c          312 g          452 t
ORIGIN      
        1 tggattccct gaaatgttaa cattcgtaca aatgggcaat gtgattatat tttcgggtaa
       61 tgaagttagt aggttgttgg aaacatccag tgccaatagt tcttgcggta atctggataa
      121 tctgcgtagt tgattttcac ctaccttcaa atattttaag ctctgaggaa attctggtag
      181 ttcctgtagt ctgttgccgg aaacaaagag ttcttgtagt ctatgaggca attcaggcaa
      241 gacagtaagg cgattacctt caatacgtaa tattgttagc gttgatggaa gctctggtaa
      301 cgtctccaga ctattaaaac gtgccgagag tgattgtaaa tatggtggta atgatggcag
      361 acatgacaaa tttcgattga atgaaatatt aagcttaatc agttgagatg gaagtgtcgg
      421 gagtatattc aagttgttac catttacatg aagttctgtc aaaagcggag aattttcagg
      481 tagggagatt aactcattgt tggaaatatt aagttcacgt atatgcaaag ggagtggtgg
      541 taaagaacgt agtttcagtg agcttaaatt gagaacaggc tcccgggtat ccagacatat
      601 tttcaataat cgaaccgcct ggatgcgatt ttctgttgtt gttccttccc tagcccagtc
      661 atcccagatg cggtggtaat ctctggtaca ctgttcttct accgataaac gagatataca
      721 tgataagcca caaagggaac gaaaaaaaga atgattgttg atcggtttca tttattaacc
      781 cctcactatt catatcttgg tctattaatg caaataaaac gagaaagtct ttaaaaaaac
      841 aatcctagag ctttatcggc gttttcattc cggctggagg aatatgttcc tgtctttcca
      901 gtaaccgtcg attgttatat cagtctaccc atgttaatgt gaccagttct actttcatcc
      961 agtttggatt gatctttatc ccctatattt tcaaacaatt tttatcattt aactcattac
     1021 tatctcgata ttcccatagc aaacgatctg gttggttatc ccctcaaaaa agtcagttgc
     1081 acgccgtaaa tccctgccgg tattgagcga ggatttacgg gttgttgtta aggtccgtaa
     1141 tcgggtcaca gtttttccgg agtcaatccg gtctgcggtt tatgcttatg cgacgtgatt
     1201 atgaatggtg cagtcgtgag ccgttttcag acaatcgcag ggccagtacc tcgtcagtca
     1261 actgacggta aatctgctgt tcagtctcac gcatcacctg tgcaccggct tccctctccg
     1321 catccgcatc accgctcaga cctgatgctt tcagccggtc agccaccctc tgagagtact
     1381 cattctccag catctcatac ttctgctctt ctgcctgcgc ccagcggtca gcttccgtac
     1441 gcttcagtac agcat
//
LOCUS       DAJTUP010000266         1454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTUP010000266 DAJTUP010000000
VERSION     DAJTUP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.273"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1333)
                     /gene="ospC2"
                     /locus_tag="OO468_004160"
     CDS             complement(<1..1333)
                     /gene="ospC2"
                     /locus_tag="OO468_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR6261034.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNK"
BASE COUNT          457 a          239 c          222 g          536 t
ORIGIN      
        1 ttttgtttac atcaaccaat ccattactaa taaaatattc tagcacttta tagttggcac
       61 tatcttcact aagtaaatac tcaacatcat acagctcaca atacatattg ttgttcattt
      121 tctccatctc tgcgatatct tgttttgtaa aattaaattt agatagtaaa taaagtgcca
      181 tatcagattt tgcattgcct agagaataag caagagcttg atgagccatt tttttatcag
      241 tcaccttatt gttaatttct tcataattgg atgctttaat ggcatcctcc aaagatatat
      301 ctctgagtct tatctttttg aagttatctg tgcttagcat tcttggtata tgatactcaa
      361 gctgaaacac aaagtttata attctatcca gactaacagg atcaatgttt ttattataac
      421 aaaactctcg gaatctttga tctttactct ttcttataaa atcaataaga tgcaacccaa
      481 ttccaagcct catatcttta gtattaaata ttggcacatc agtttttccc tgattaccat
      541 gcacatacct gctaacttca tccacaacct ctttaaagtt atctaagaaa aatgattggt
      601 gctcatggta aaaaacaggt ggaatagcat tatcaaagtg gtcggttaat gtcatatatc
      661 catatggaga gtcatgatca atgatatacg cattagcacc aaaatccaca gtatgatgtg
      721 ttttatttag tgggagtgtt tcttgatgat tggaaatttc aaccccaaaa aaaacaaaat
      781 cgtggttagc tatttcttca atatcctcag agaatgtgtt tttatatgtt tgtatattct
      841 cagacttaat tttatcaaag gacttaatat taagagtatt atttgacagg ttagcgttac
      901 tttgatgtct gaagttaaaa tcagaattaa taataataga gagcaagtga cgttcatccg
      961 aagaaagagg atgagttttt tgtattattt cagaaagata gtgtctggac accttatcga
     1021 ggtgctcaag tgaccgaaat gatgaccgtg ctccagatgg cgcttcaata gaaagattta
     1081 atgatttgaa actggtccct tgagagaaca ttttactata actttgcgct gctatagact
     1141 tccttaaaaa gcttttcaca gtatcagcac agtgatctaa atgtttttca cttatgccag
     1201 aagaggatga gtttgttacg catgatatgt ttttttgtaa tgcttttgta tctttgttcg
     1261 gatttatttt tccatcaaca agatcaatat tgttctgaac attaatatga tttactgctt
     1321 caggtatttt cataaaacat cccccattat gtgcgttttt atctgcagtt atttagaata
     1381 atattatcta acttaatatt ttcttgtatg tattataacg aacatatttt aatatgttgt
     1441 acgattttca ctct
//
LOCUS       DAJTUP010000267         1433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTUP010000267 DAJTUP010000000
VERSION     DAJTUP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.274"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            82..1275
                     /locus_tag="OO468_004161"
     CDS             82..1275
                     /locus_tag="OO468_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR6261035.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(1295..1433)
                     /locus_tag="OO468_004162"
                     /pseudo
     CDS             complement(1295..1433)
                     /locus_tag="OO468_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          421 a          310 c          336 g          366 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac cattccatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt ggtcttcggg ccagcaaaag tcgttcaaca
     1021 ggaagaattc tggttctgag tctgctggca acgttgagca ctatcgtaat gtggttactc
     1081 ggttatcacg ctgaaaataa agggttacac ctgaaatatc aggctaacag cattaaatcc
     1141 cggcgggtta tctcatatct gacgttagcg aagaatgttc ttcgacactc gccacttatt
     1201 ttaagacgaa cagtactgag cacagttcta aaccatctgt ccagaaccta ccgaaatatg
     1261 gtgctggttt attagcagta attttgtggg gatccctcag cctgaagggg cgacacagtc
     1321 ggggagagaa gcccatctcc ttgccgaact gccacagcga aggatggaac cggtgctgac
     1381 cggtctgata tgcgtcacgt tgcagaacca cagttttcat attgtcatac aac
//
LOCUS       DAJTUP010000268         1410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTUP010000268 DAJTUP010000000
VERSION     DAJTUP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.275"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            365..>1410
                     /locus_tag="OO468_004163"
     CDS             365..>1410
                     /locus_tag="OO468_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR6261036.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          459 a          306 c          225 g          420 t
ORIGIN      
        1 acattgagat atgattttga tcattactaa atcctttgct gaagttacta tgtatataag
       61 cttattttat acatgattat tagcaacaaa tattaccaag tcaagtagtc aggctggcta
      121 aaatagttgg agatgttgta gtatcttact caagactaat gtcctctggt ataaatcctc
      181 tgcattgtga atatgtaaaa catcaaaaaa agatgaacca aatatacgaa aaaagcaaat
      241 atacttacta gggtagaaag catgatataa cactcattcc cttatggcga atattctgaa
      301 acaccctcct cattaggaga gatacattaa atacaattta ttcacaatct aattccatat
      361 ccatatgcga gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag
      421 cacaattaca tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt
      481 accggaagaa cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg
      541 gcataaactg gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat
      601 taaaaagctt gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat
      661 taaagaactt attgcaagag ataatttctt aacacatata tctgcattac cacattatct
      721 aataactttg gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat
      781 caaatcacta agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt
      841 aaaaaaactt gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct
      901 taagatactt aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact
      961 gcaacttctt tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat
     1021 tatcatgaga agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct
     1081 ttttcgttta gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac
     1141 tctgctattc ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat
     1201 ttccctgtca gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc
     1261 gcgccatatc agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga
     1321 aactgtggca gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc
     1381 tttttccacc gaggagaatt cccgggcatt
//
LOCUS       DAJTUP010000269         1398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTUP010000269 DAJTUP010000000
VERSION     DAJTUP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.276"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            124..375
                     /locus_tag="OO468_004164"
     CDS             124..375
                     /locus_tag="OO468_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261037.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            442..720
                     /locus_tag="OO468_004165"
                     /pseudo
     CDS             442..720
                     /locus_tag="OO468_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            722..>1398
                     /locus_tag="OO468_004166"
     CDS             722..>1398
                     /locus_tag="OO468_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR6261038.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          348 a          348 c          370 g          332 t
ORIGIN      
        1 tattttcaac gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta
       61 gcgcgcaggg ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt
      121 ctgatgatga acatcgaaga actgcgtaaa attttttgtg aagatggcct ctatgctgtg
      181 tgcgttgaaa atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg
      241 gctgcgttaa ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt
      301 gagtttgtca cttcattaca ggcattgaaa gagatcggaa taaaagctgg cttttctgct
      361 ttttcagaag aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca
      421 ccgtgccacc ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg
      481 accgttcttg cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag
      541 catccctcat gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga
      601 tgcgaggtta aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc
      661 ccttactccg aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa
      721 cgtgcgggta ttacttcgac ctgttctggt tccggaactc gggctggtgg tccttaagcc
      781 cggtcgtgaa tccatgccgg tattccacaa tacccgggta ctggtggagc cggaaccgaa
      841 aagcatgcgt aatctgccgt ccggggtcgt tcctgccgtt cgccagccgc tagtggaaga
      901 caaaacattg ctgccgtttt tcagtaacgc acgggtgatt cgtgctgctg gtggtgctgg
      961 cgcattgtct gactggctgt tgcgccatat taaatcctgc cagtggccac acggcgatta
     1021 tcatcacagc gaaaccgtca ttcaccgtta tggtaccggc gcaatggtat tgtgctggca
     1081 ctgcgacaac cagctgcgtg accagacatc cgaatcactc gagcaacttg ctcatcaaaa
     1141 cctgtcagca tggatgattg acgtcatcgg tcacgcaata agcggtacgc aggagcgtga
     1201 attatctctg gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc
     1261 ggaagcggta ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg
     1321 tgaaagcgac atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa
     1381 aaatcttgcg ccgctgcc
//
LOCUS       DAJTUP010000270         1390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTUP010000270 DAJTUP010000000
VERSION     DAJTUP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.277"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            61..333
                     /locus_tag="OO468_004167"
     CDS             61..333
                     /locus_tag="OO468_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR6261039.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(299..706)
                     /gene="nudG"
                     /locus_tag="OO468_004168"
     CDS             complement(299..706)
                     /gene="nudG"
                     /locus_tag="OO468_004168"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR6261040.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            793..>1390
                     /locus_tag="OO468_004169"
     CDS             793..>1390
                     /locus_tag="OO468_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR6261041.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWFAWIFGALCWMTTFTRVWSG"
BASE COUNT          292 a          343 c          378 g          377 t
ORIGIN      
        1 aagttttgcc acccggctac cgttatcagt agcagagaca ataacgttaa ggagttccct
       61 gtgagtcgag cattgttcgc cgtagtatta gcatttccat taattgcact ggctaacccg
      121 cattatcgtc cggacgtgga ggtaaacgta ccgccagaag tgttcagttc aggcgggcag
      181 tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc aaaattattc tgaaggggcg
      241 gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg atgacaaaac ccttagcact
      301 aatccgctgc tctggcggcg cgtaaagcca taaacgcctc taataatgga atgtcagcag
      361 gggccagcgg atattgcagc gcctcttcag gtgagcacca gaccagcgcc tgatgttcat
      421 gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc atgaagatgg ataatccggc
      481 ccgaaacttc tcgctgatgg ctggcaacat actcacccac agttgcttcg atgtccaatt
      541 cttcgtttaa ctcacgcacc agcgcctgct gctggctttc atccagctcg actttacctc
      601 cggcaaactc ccataatccc gcctgatcgc tctgggcggg gcgttgcgcg agtaaaattt
      661 tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat tttcataaat tacacacggg
      721 cagataagcg aaaaatgcca taatatccgc actgttgctc aatcagaaag gctttatatt
      781 taaggagtgc ctgtgctaga ccgccatctt catccccgga ttaaaccgtt gttgcatcag
      841 tgcgtgcggg ttcttgataa accgggcatt acgccagatg gtttaacatt agtgggattt
      901 gccatcggcg tgctggctct gccgtttctg gcgctgggct ggtatctggc ggcgctagtc
      961 gtcattttgt tgaacaggct gcttgatggt ctggacggtg cgctggcgcg gcgcagaggg
     1021 cttaccgacg cgggcggctt tcttgatatt tctctcgatt ttctctttta cgcgctggtg
     1081 ccgtttggct ttattcttgc tgcaccggag caaaatgcgc tggcaggcgg ctggttgttg
     1141 tttgcgttta tcggcacggg cagcagtttt ctcgcctttg ctgcactggc agcaaaacat
     1201 cagattgata accccggtta tgcacataag tcgttttact atctgggggg attaaccgaa
     1261 ggcacagaaa cgatcttact gtttgtgctg ggatgtttat tccctgcatg gtttgcgtgg
     1321 tttgcgtggt ttgcgtggat cttcggtgcg ttgtgctgga tgacgacctt tacgcgggtg
     1381 tggagtgggt
//
LOCUS       DAJTUP010000271         1390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTUP010000271 DAJTUP010000000
VERSION     DAJTUP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.278"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..1292)
                     /locus_tag="OO468_004170"
     CDS             complement(<1..1292)
                     /locus_tag="OO468_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCR6261042.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADS"
BASE COUNT          421 a          362 c          333 g          274 t
ORIGIN      
        1 gaatcagcag acgttacaaa gaagacgatg accatcgcca ttgcaatgaa tgacagcaca
       61 gaagagaacg ggaaatgctc caggaaatta aacagggcca gcgccacatc ctgctgaaca
      121 gtattggcga ggtctgtggc cccctggttc ataatgagat agatcgcgct gttaccaaac
      181 accgtcatcc acattagcgt aaaacccgcg ggaacaaaca gcacgccggt gacaaactcg
      241 cgaatggttc gcccgcggga gacccgtgcg atgaacatcc ccacaaacgg cgaccatgaa
      301 agccaccatc cccagtacag taatgtccag ccccccagcc agttgctcga cttgggctca
      361 tacgcgtaaa ggttgaacgt tttactcacc agttccgaaa gataaccgcc cgtattttcc
      421 acaaatgact tcagcagaag cacggttggt cccagacaca ggaccagcgc caggagcaac
      481 aaagccagac ccagattgag ttcagacagg atacgtattc ccttatccag accggacacc
      541 actgaaatcg tcgctaaccc cgtgatgacc acgatcagaa ttacctgcac cgtttcattg
      601 atgggcaccc cgaaaagatg gttcaaaccg gcattcacct gcaaaacacc gtaacccagt
      661 gatgtcgcaa cgccaaagac cgtgcctata acagcgaaaa tatcaaccgc atgtcctaca
      721 ggtccgtata tgcgatcgcc aataatggga tagagtgcgg agcgcagagt taaaggcaga
      781 ccgtgacggt aactgaagaa cgccagaatc agcgccacaa tggcataaat tgcccatgcg
      841 tgcagtcccc agtggaaaaa ggtcagacgc attgcttcct tagctgccgc aacggtttct
      901 ggagtgccaa cgggtggcga aagataatgc attacaggtt cggcaacgcc aaagaacatc
      961 aggccgatcc ccatccctgc cgaaaaaagc atcgcaaacc aggagtggta gctgaaatca
     1021 ggctgcgcat ggtccgggcc cagcttgata tcaccgtagc gtgagagtcc aaggaacgtg
     1081 acactcagta aaatcagggc cacagcaagg atgtagaacc agctggcatt cgtgaagatt
     1141 tgttgctgaa gtagtttaaa atttttgtcg gcgacatccg ggaatacggc ggcaaaggcg
     1201 acaagaagga aaattagcaa agcagatgta aagaataccg ctttgttaat ctgacttgta
     1261 gacttctttg ggattgtatc attttcactc ataatcattt attccattaa ttaaattcgc
     1321 actggataga aggtactacc tctcacaaag tagcaaagtt caactggcta tgcggcgtta
     1381 atcgtaatgc
//
LOCUS       DAJTUP010000272         1378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTUP010000272 DAJTUP010000000
VERSION     DAJTUP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.279"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..194
                     /locus_tag="OO468_004171"
     CDS             <1..194
                     /locus_tag="OO468_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HCR6261043.1"
                     /translation="EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIP
                     ALVAFALPPRAGATASRSA"
     gene            252..>1378
                     /locus_tag="OO468_004172"
     CDS             252..>1378
                     /locus_tag="OO468_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR6261044.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
BASE COUNT          365 a          337 c          378 g          298 t
ORIGIN      
        1 aggaaatcgc cttcgatatg agcgcggtcc tctcaactca ctcgctgttt ggcacgctga
       61 tggaagggat ttttggctat caggaagcac cgagcgtcag cgaagtcgcc gtctggttta
      121 tttatctcat cccggcgctg gtggcatttg ctctgccacc acgcgcaggg gcgacagcgt
      181 ctcgctccgc gtaacaaata cgacgcaaac tcttgcttag ttacaacata ctttaaaggg
      241 atagtctcgt catgaccatt aacttccgcc gtaacgcatt gcagttgagc gtggctgcgc
      301 tgttttcttc tgcttttatg gctaacgccg ctgatgtgcc gcaggtcaaa gtgaccgtga
      361 cggataagca gtgcgaaccg atgaccatta cggttaacgc cgggaaaaca cagttcatta
      421 ttcagaacca tagccagaag gcgctggagt gggagatcct caaaggcgtg atggtggtgg
      481 aagagcggga aaatatcgcc cctggtttta gccagaaaat gacggcgaat ttacagcctg
      541 gcgaatacga tatgacctgc ggtctgctga ctaacccgaa agggaagttg atcgtcaaag
      601 gtgaggcaac ggcggatgcg gcgcaaagtg atgcgctgtt aagtcttggt ggtgcaatta
      661 ctgcatataa agcgtatgtc atggcggaaa ccacgcagct ggtgaccgac accaaagcct
      721 ttaccgacgc gattaaagca ggcgatatcg aaaaagcgaa agcactgtat gcgccgacgc
      781 gccagcacta tgagcgtatt gaaccgattg ctgaactgtt ctccgatctg gatggcagca
      841 ttgacgcccg tgaagatgat tacgagcaaa aagccgccga cccaaaattc actggtttcc
      901 accgtctgga aaaagcattg tttggcgaca acaccaccaa agggatggat cagtacgctg
      961 agcagcttta taccgatgtg gtcgatttgc aaaaacgcat cagtgaactg gctttcccac
     1021 cttcaaaagt ggtcggcggc gcagccggac tgattgagga agtggcagcc agcaaaatca
     1081 gcggtgaaga agatcgctac agccacaccg atctgtggga tttccaggct aacgttgaag
     1141 gctcgcagaa aattgtcgat ctgctgcgtc cacaactgca aaaagccaac ccggaactgt
     1201 tggcaaaagt cgatgccaac tttaaaaagg tcgataccat tctggcgaaa taccgtacta
     1261 aagacggttt tgaaacctac gacaaattga ccgatgccga ccggaatgcg ctgaaagggc
     1321 cgattactgc gctggcggaa gatctggcgc aacttcgcgg tgtgctggga ctggatta
//
LOCUS       DAJTUP010000273         1370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTUP010000273 DAJTUP010000000
VERSION     DAJTUP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.280"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            400..1149
                     /locus_tag="OO468_004173"
     CDS             400..1149
                     /locus_tag="OO468_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR6261045.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          388 a          279 c          275 g          428 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcactatc
//
LOCUS       DAJTUP010000274         1364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTUP010000274 DAJTUP010000000
VERSION     DAJTUP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.281"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            69..1214
                     /gene="ssuD"
                     /locus_tag="OO468_004174"
     CDS             69..1214
                     /gene="ssuD"
                     /locus_tag="OO468_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR6261046.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            1223..>1364
                     /locus_tag="OO468_004175"
     CDS             1223..>1364
                     /locus_tag="OO468_004175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR6261047.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVV"
BASE COUNT          290 a          366 c          409 g          299 t
ORIGIN      
        1 ctggtgccga aaaaagtcga tattcgccag cgcatctggc agcccactca actggaagga
       61 aaacaattat gagtctgaat atgttctggt ttttaccgac ccacggtgac gggcactatc
      121 tgggaacgga agaaggttca cgcccggttg atcacggtta tctgcaacaa attgcgcaag
      181 cggcggatcg tctgggctat accggtgtgc tgatcccaac ggggcgatcg tgtgaagatg
      241 cgtggctggt tgcagcgtcg atgatcccgg tgacgcagcg gctgaagttt cttgtcgccc
      301 tgcgtcccag cgtaacctca cctactgttg ccgcccgcca ggccgccacg cttgaccgtc
      361 tctcaaatgg tcgtgcgttg tttaatctgg tcacaggcag cgatccacaa gagctggcag
      421 gcgacggcgt gttccttgat catagcgagc gctacgaagc ctcggcggaa tttacccagg
      481 tctggcggcg cttattgctg ggggaaaccg tcaatttcaa cggtaaacat attcatgtgc
      541 gcggagcaaa actgctcttc ccgccgattc aacagcctta tcctccgctt tactttggcg
      601 gatcgtcaga tgtcgcccag gagctggcgg cagaacaagt tgatctctac ctcacctggg
      661 gcgaaccgcc ggaacttgtt aaagagaaaa tcgaacaagt gcgggcgaaa gctgccgcgt
      721 atgggcgcaa aattcgtttc ggtattcgtc tgcatgtgat tgttcgtgaa actaacgacg
      781 aagcgtggca ggccgccgag cggttaatct cgcatcttga tgatgagact atcgccaaag
      841 cacaagccgc attcgcccgg acggattccg taggacaaca gcgaatggcg gcgctacata
      901 acggcaagcg cgacaatctg gagatcagcc ccaatttatg ggcgggcgtt ggcttagtgc
      961 gcggcggtgc cggaacggcg ctggtgggcg atggtcctac ggtcgccgcg cgaatcaacg
     1021 aatacgccgc gcttggcatc gacagctttg tgctttcggg ctacccgcat ctggaagaag
     1081 cgtatcgggt tggcgagttg ctgttcccac atctggatgt cgccattccg gaaattcccc
     1141 agccgcagcc gctgaatccg caaggcgaag cggtggcaaa tgattttatc ccccgtaaag
     1201 tcgcgcaaag ctaaggaaca cgatgatggc aacgccagtg aagaagtggt tattgcgcgt
     1261 tgccccctgg tttttaccgg tgggcatcgt ggcggtgtgg caactggcct cctcggttgg
     1321 ctggctgtcg acgcgtattt tgccttcacc ggaaggagtg gtga
//
LOCUS       DAJTUP010000275         1318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTUP010000275 DAJTUP010000000
VERSION     DAJTUP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.282"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            110..319
                     /locus_tag="OO468_004176"
     CDS             110..319
                     /locus_tag="OO468_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR6261048.1"
                     /translation="MAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKGSDFTQ
                     ALLGHRSSSMTDKYRDGRGREWKDI"
     gene            555..>1318
                     /gene="ybhA"
                     /locus_tag="OO468_004177"
     CDS             555..>1318
                     /gene="ybhA"
                     /locus_tag="OO468_004177"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR6261049.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIV"
BASE COUNT          372 a          311 c          320 g          315 t
ORIGIN      
        1 ccccaccacg atttccagat tgaatttaac acttgccgat gtactggata acttgaaaaa
       61 aataaacggt aaaaacgaaa agttactcgg agggaagaca gccaggacaa ttgcagcaca
      121 gttcaggatt gcccgagaca catccggatt gaaatgggaa ggtgacccac cgccatttca
      181 tgaaatccga agtttatccg gtcgcctgca ctcagcagaa aaaggcagtg atttcactca
      241 ggcgctcctt ggacatcgtt catccagcat gacggataaa taccgtgacg gacgagggag
      301 ggaatggaaa gacatttaga attaatttcg aatgatttcg aaatgccact tgtaattaat
      361 tgattataaa gaattaattc ctaaagagca tttgcttcat gaatgctcct ttgcatttag
      421 caactgactg taacagctaa aattagtcgc ttttggcggt gaatgctgaa ttaaaacgca
      481 gaattttgtc cgatagtggt atcggtgtac cattcgcgca gacgaaaatt cttaacctta
      541 atctggaata cgccatgacc acacgcgtga ttgctctcga cttagacggc accttattga
      601 ccccgaaaaa gaccttgctt ccatcatcga tagaagccct ggcccgcgct cgcgaagcag
      661 gctatcaatt aatcatcgtc acaggtcgcc atcacgtcgc tattcatcct ttttatcagg
      721 cgctggcgct ggatacacct gctatttgct gtaatggcac ctatttgtat gattatcatg
      781 caaaaaccgt gctggaagcg gacccaatgc ccgttaataa agccctgcaa ctcattgaga
      841 tgctgaatga acaccacatt cacggtctga tgtatgtcga tgatgcaatg gtctatgagc
      901 acccgaccgg gcatgtcatt cgcacgtcta actgggcgca aaccctgccg ccggaacagc
      961 gtccgacttt cacacaagtc gcttctctgg ctgaaacgac gcaacaagtt aacgccgtat
     1021 ggaagttcgc cctgacgcac gatgacctgc cgcaattgca gcattttggt aagcatgtcg
     1081 aacatgaact gggactggag tgtgaatggt cctggcacga tcaggttgat attgcacacg
     1141 gcggcaacag caaaggtaaa cgtttgacga aatgggttga ggcgcaaggt tggtcgatgg
     1201 aaaacgtcgt ggcattcggc gataacttta atgatatcag tatgctggaa gccgctggta
     1261 caggcgtggc gatgggcaac gccgatgacg cggtaaaagc gcgcgccaac attgtgat
//
LOCUS       DAJTUP010000276         1237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTUP010000276 DAJTUP010000000
VERSION     DAJTUP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.283"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..218)
                     /locus_tag="OO468_004178"
     CDS             complement(<1..218)
                     /locus_tag="OO468_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCR6261050.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLV"
     gene            complement(284..523)
                     /locus_tag="OO468_004179"
     CDS             complement(284..523)
                     /locus_tag="OO468_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR6261051.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(658..>1237)
                     /locus_tag="OO468_004180"
     CDS             complement(658..>1237)
                     /locus_tag="OO468_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR6261052.1"
                     /translation="LGQGVKFYPASRRITTSTGNVINLSENGYRFLLLLLQGESDKQH
                     IINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRYLGKVEKHVC
                     ENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTDKNIEWYRTK
                     QWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          368 a          278 c          217 g          374 t
ORIGIN      
        1 acgagcaaaa cgctcccggc tgcaaccaga ttgggttttt gatgctgtaa aatgagtgcc
       61 aaaaaaataa gcacggttaa caccagtaaa taacgatgac taataattct gagtaatata
      121 tccccccatg aaataaaaat aagaaagagc gcaacaaata aaagatcgac tcttagtaac
      181 catattgctg ccattgaaag actccatgta ccatacaagg ttgctgccag acatattcct
      241 ttacgtctga cagcctctag agagtttttt tattttttat aagttacgga ttactcgttc
      301 cggaaatcgt tacctgttga attgctgcag cgatagcgtc gaatgcatct ttaattgcgc
      361 ccaggaagcc tgagttctga tcgccaaaga tgaaagcaag cagagttgcc atagcaacac
      421 caatcaatgc atattcgatt gcagtaacac cacgttcatc cttcgcgaaa cgcgcaaagc
      481 actgtttagt tttaccatag tacttagtaa taaaagtatt catgttttcc tcgtatatta
      541 ttatttaaat tcaaaccacc agcttcatct gcgtgtcacc ggtatcccat atttttggga
      601 tgtggctata attatctatt cccgctattt agtcttcaaa aaaaattaca tttaatatca
      661 tttatcttga ttaagaaata gtaagactaa aaaaacaagc gtcgtcagcc agcacactgc
      721 taacacggta agaacgctaa taaaaacact ccactgcttc gttctatacc attcaatatt
      781 tttgtcagta tgttttattg ggagcagctc ttcagtagag aattcctggc tgttatcatt
      841 ctcaggaatg tttgcatcag tgtttacttc cctggcctcg ctatcgttgg tatagctact
      901 actgtcttca tcactgtcac gctcattctc acagacatgt ttctcgacct ttcccagata
      961 tctgacgcct tttcgcggaa tggttttaat tagagaactc gacaaacctg cgacgtctaa
     1021 tgcctttcta agcatgtaaa tctgcccata ataactgctg tcgcttaccg aacctttctg
     1081 ctcccgccaa acctcattga taatatgttg cttatcggat tctccctgta gtaaaaggag
     1141 taaaaatcga tagccgtttt cagacagatt gattacattg cctgtactgg tggtgatacg
     1201 tcgagacgct gggtaaaact taactccctg ccctaaa
//
LOCUS       DAJTUP010000277         1220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTUP010000277 DAJTUP010000000
VERSION     DAJTUP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.284"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..684)
                     /locus_tag="OO468_004181"
     CDS             complement(<1..684)
                     /locus_tag="OO468_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR6261053.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDI"
BASE COUNT          323 a          188 c          302 g          407 t
ORIGIN      
        1 tatgtcaaaa taaccgttcc ttaaattttc cggcaggtat ggaatatcgg ttaacatatt
       61 gtcgattgcc catatggagt gaatgttgtg aggtagaggg gggagttcac taagaagatt
      121 ccctattgcg ctaatctctt gtaaagaaaa tggtaaaggt ggtaaaactg ccagcccatt
      181 acatgataca tctaatgttt ccagtagttc tggtaaaaca ggaagagaac ataattgatt
      241 atttgagaca tgaagctcct tcaaagatat ggggagtgtg ggtagtgtga ttagttgatt
      301 gtgggacgca ttcaataatt taagtccttg aggcaaagca ggcagttcaa taagtctgtt
      361 atagctgaca ttaagttgtg taagggacgc aggcaatggg gagattaagc ttaaattatt
      421 tttacttata ttaattgatt taattcccgg ggggatttca ggtaatgttg tcaggcctaa
      481 ttcagacaag tccaggttcg tctcttggtt ttgtagacat gatactagtc gctgaaaagc
      541 gatgtctcgt tgttcttctt gtatgcggtt atttttccat tcagtccaat gggttagata
      601 attttcatat gcgcggctag tgtcgattga ataagtggag aacgaatttg aaattaatct
      661 gtgattgtta tttgtcggga gcataaatat cagggtgtat tctttatgtt gagaggtgtt
      721 ggattctttt ttatctgctt tgcaatgtta ttggttccct tcagcagtag acaggaactt
      781 ctggaggagg cggatatata gtttgtgtaa gcatattaac tccatatgtt atatattgaa
      841 gaacttctgc tttacactat cctaaactgg atcggtttaa tgtaaaaaaa cgatactttt
      901 tggggggggg gcaatttttc cttaaggtat gaaaaaagga gctgaagcta tataatagct
      961 attaatgcca ctggtatcag tgaatcatgt atataaaaaa ctgtgttaag gaggctgtcc
     1021 attagcggtt ttttattctt ctggaaccgc ggcatctttg cgtatctttt tgtcttctca
     1081 ccgtctggtc ggtgttttgc tgagactgct aatttcctgt ctttgctagt gctgtactgg
     1141 tactgcccaa tcatgattgg cgggggcggg cggagttgag accgcagaca cgtcgtatgc
     1201 aagaacgtgc tgcggctggc
//
LOCUS       DAJTUP010000278         1194 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTUP010000278 DAJTUP010000000
VERSION     DAJTUP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.285"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..635)
                     /locus_tag="OO468_004182"
     CDS             complement(<1..635)
                     /locus_tag="OO468_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR6261054.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLW"
     gene            complement(632..994)
                     /locus_tag="OO468_004183"
     CDS             complement(632..994)
                     /locus_tag="OO468_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR6261055.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          349 a          271 c          241 g          333 t
ORIGIN      
        1 ttccataatt tccagccatt aaattttgag atgccagcaa cacgctctgc gcgtacatat
       61 tcaacgacat ccgtcggacc acccacccag ctcagtatgc ctttcatgaa aagattgcgc
      121 tcaggcaaca gtttaatgtt ctccacaacc tcacgagaca tgagtcgaaa atctccgaca
      181 ttttcctcga tctttggggt gcttattttg ttgtgtaatt tatagaacca ctcagctgtc
      241 ttacgtttca gtcgtccatc agttgagcgg tctgaacgtt tagcaagcac catgtcagca
      301 cctgcctgcc acttttcaat aagatgaggg ataacctcaa ttgggtcttg caggtcgaca
      361 tcaataggaa ttacagcatc gccgcttgca tggtctaacc ctgcaaataa ggctggttct
      421 ttaccaaagt tgcgtgtaaa tgacagcgga acaactagcg ggtctgaaac agccagcgcg
      481 ttaataattg actctgtggc atctttactt ccgtcattta tgaatacaat ttctacttca
      541 tatggcttca actcttggaa ttcacgtacc gttttataga aaacaggtat cgcttcttct
      601 tcattgaaga caggaacgac aagagatatt ttcatttcgc atccctaaag acaatgaact
      661 ttgaatagac gaaaccgcac accagactga tggcggagaa ggtgataaga gtgactattg
      721 gaggaagtga gcatttatca gctgcccaac caacaatcac actcaatatt cccataaatc
      781 ccacgtataa catgtagcgc atcgctgtag tcaatgcttt gaatgtgaat cttgcattcg
      841 cgaagaagct aaagctcaca gccactacga aacctgtgaa gtttgccaga gcctgactgg
      901 tatgtgcggc atagatacat acaccaaaaa ccacccagtg tataagtgtg ttcagcacac
      961 ctatagatgt gtactttaca aataacttta acatttattt aatcaatgag ctctgaaagg
     1021 catgaagtct atcatccaag tctcaattga tcgatacttg ctatgtctga tgagacaaaa
     1081 ctgagacaca taaggcctca caatggcttg caaggcttta catgttttga tgtggtggga
     1141 cgtgtgagcg cagtgttgat gggataatcc tttgaattac aagcggattc ttat
//
LOCUS       DAJTUP010000279         1176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTUP010000279 DAJTUP010000000
VERSION     DAJTUP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.286"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..486)
                     /gene="ipaH7.8"
                     /locus_tag="OO468_004184"
     CDS             complement(<1..486)
                     /gene="ipaH7.8"
                     /locus_tag="OO468_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR6261056.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FL"
     gene            815..994
                     /locus_tag="OO468_004185"
                     /pseudo
     CDS             815..994
                     /locus_tag="OO468_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1011..1176)
                     /locus_tag="OO468_004186"
                     /pseudo
     CDS             complement(1011..1176)
                     /locus_tag="OO468_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          363 a          180 c          252 g          381 t
ORIGIN      
        1 taaaaatttc aatttttcgg gaagagccgg taagttatat agcctgttat tttcaacaaa
       61 taatgatgtt aaatggtttg gcaaaacagg aaggttttcc agttgattag aacgaacact
      121 taaagttgtt aatgactcag gaagttctgg tatcacagaa agcctgttat tatcagcatt
      181 aagttcttta agaaatgcag gcagctttgg caatgatgtt aatttattat tttctacaat
      241 caaagcagaa atatgctttg gtaattcggg aagagacgtc aaatttaggt ctgataaatt
      301 taatactgct tcttgattct gaaagcactg ggagagtctg ttaaaagcaa tgcctcgctc
      361 ttccccggga gaagagttct tttcccattc agtcaggatt ctcagataat gttcattact
      421 ggttgagttt gagttaatag agggggagca agaaactgat gagtgtgtat tatttacaga
      481 gaacataatg ctaggtcaac cttatatttg tgagaataaa tatttaaaat tgtatattta
      541 tacaaatttc acttaccatt tccgatatta agttttaata gtttccattt tacacataac
      601 agaagttagt tgtaatttat ttatggaggt tttctatgga attctttttt tattgtggct
      661 gcagtcaata ataacactga attcaacatt ccagtgagca aggaaatgta agccgagtaa
      721 gtatatttgt tttacatcat attttagtca agctcattta ttcctattat agaatgacaa
      781 attaaaatta ctgtaaaaaa acgatacaca tttaatacga gggaggccgg cagaaaaacg
      841 gcttgcggtc aggcaggaga aatgtgtgcc gcgaatgaag tccttgtatg actggataaa
      901 gctccagagg aaaactttgt caaaccatgc tgaaatggta aaggcgttca actatatcct
      961 gaatcactgg aatgcactga atgagttttg ccgggtagta cttccaatca ttagaatatg
     1021 tgcttttcaa tgaaggaacc gatgactttt tcgtggattt ccacggaacg cgagaagcag
     1081 attgttttac gtgccagccg cttgatgcgg gggcggaggg tcaggttgtt acgttcatac
     1141 gttgcgtgaa gattttgccg gtcaggtgtt tctcct
//
LOCUS       DAJTUP010000280         1162 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTUP010000280 DAJTUP010000000
VERSION     DAJTUP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.288"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            14..1054
                     /gene="gatD"
                     /locus_tag="OO468_004187"
     CDS             14..1054
                     /gene="gatD"
                     /locus_tag="OO468_004187"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR6261057.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT          287 a          245 c          316 g          314 t
ORIGIN      
        1 atcaggagtt tttatgaaat cagtagtgaa tgatactgat ggtatcgtgc gcgttgcaaa
       61 aagcgtcatt cctgaaatta aacatcagga tgaggtgcgg gtaaaaattg ccagctcggg
      121 cttatgtggt tccgatttac ccaggatatt taaaaatggt gcacattatt atccaataac
      181 gttaggccat gaatttagcg gctatattga tgcggtggga tccggtgttg atgatttaca
      241 ccctggcgat gcggttgcct gtgtgccgtt attaccctgt tttacttgtc cagagtgttt
      301 gaaagggttt tattcccagt gcgcaaaata tgattttatt ggctcgcggc gtgatggtgg
      361 atttgctgaa tatattgtcg ttaagcgaaa aaatgtcttt gctctaccca cggatatgcc
      421 tattgaggat ggggctttta ttgagccgat taccgttggc ctgcatgctt ttcatttagc
      481 gcaaggttgt gagaataaaa acgttattat tattggtgcc ggaaccattg gcctgctggc
      541 tattcagtgc gctgtcgcgc tgggagcaaa gagtgtgacg gcgatcgaca ttagctcaga
      601 aaaactggca ctggcaaaat ctttcggtgc gatgcaaaca tttaacagta gcgaaatgag
      661 cgcgccgcaa atgcagagcg ttttacgcga actgcgcttt aatcagctta tcctcgagac
      721 ggctggcgta ccgcaaactg tcgaactggc ggtagagatt gccggtcctc atgcccaact
      781 ggcgctggtg ggcacgttgc atcaggatct gcatttaaca tcggcaacgt ttggcaaaat
      841 attgcgtaaa gagctgacgg ttatcggcag ttggatgaac tattccagcc cttggccggg
      901 gcaggagtgg gaaacggcga gccggttgct gacagaacgt aagttaagcc tggagccatt
      961 aatcgctcac cgtggaagct ttgaaagctt cacccaggcg gtgcgtgaca tcgctcgtaa
     1021 tgccatgcag ggcaaagtgt tgctcattcc atgaaaccgc gggccagcgt gatgctggcc
     1081 cggtattgtg caaaacagat cattcaccaa tggtccccct tcgtttacac tagccacaat
     1141 tgaatatggg taaatgacga tg
//
LOCUS       DAJTUP010000281         1138 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTUP010000281 DAJTUP010000000
VERSION     DAJTUP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.289"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..424
                     /gene="mppA"
                     /locus_tag="OO468_004188"
     CDS             <1..424
                     /gene="mppA"
                     /locus_tag="OO468_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR6261058.1"
                     /translation="VASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYN
                     EPSTFLTLLTSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPI
                     APIYQYTNGRLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(475..>1138)
                     /gene="ynaI"
                     /locus_tag="OO468_004189"
     CDS             complement(475..>1138)
                     /gene="ynaI"
                     /locus_tag="OO468_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR6261059.1"
                     /translation="ITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNF
                     FSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSIS
                     VENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFAD
                     SSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQ
                     GR"
BASE COUNT          338 a          260 c          258 g          282 t
ORIGIN      
        1 tgtagcatcg atgtggaaaa agaaccttgg cgtagatgtt aaattgcaaa atcaggaatg
       61 gaaaacctat atcgatagcc gtaacaccgg caattttgat gttatccgcg cctcatgggt
      121 gggggattat aatgaaccct ccactttcct gacattatta acgtcaatgc attcaggaaa
      181 tatttcacgc tttaacaatc cggcatacga caaagttctg gcccaggcat cgacggaaaa
      241 taccgttaaa gcgcgtaatg ccgattacaa cgcggcagaa aaaatcctca tggagcaagc
      301 accgattgca ccaatttatc aatataccaa tggacgatta atcaaaccgt ggctgaaagg
      361 ttatcccatt aataaccctg aagatgtagc gtacagtcgg actatgtata ttgtgaagca
      421 ttgatgtgat gggaactggc gttacccttg tggataacgc cagtgatgtc tgatttagcg
      481 cccctgttcc ggcggtgtaa tgttatccat atacagcgtc tggctgggga aggcaaagtc
      541 cgcgccgtgt gactgtacaa tatcgataat cttcaaataa acgtcttgct gtgcagcaag
      601 ccactcagcc cataccgtgg ttttggtaaa gcaataaacc ataatattca atgacgagtc
      661 agcaaactgg ttgaaataaa ccagtaaggt ttgtcgctgg tcgatggccg ggtgattttt
      721 cagcatctca cgtacggctt cgacaataac gcccactttt gccgcatcct cataacgtaa
      781 accaatggtc gtggtaatgc ggcggttggt cattcgtcct gggttttcta cgctgatcga
      841 cgaaaacagc gagttcggta cgtacaatgg acgattatca aaggtcgtaa ttttggtaat
      901 tcgccagcca atttccgcta ctgtaccttc gatatttctg tccggtgaac ggatccagtc
      961 gccaatactg aaaggacggt cgaaatagag catgatcccg gaaaagaaat tactcagaat
     1021 atctttaccg gccataccga cagccagacc accaatacca ccaaaggtca gcaagccaga
     1081 aaggctcatg ccgaaatgtt cgccataaag cagaacaagc actacaataa tggtgatt
//
LOCUS       DAJTUP010000282         1103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTUP010000282 DAJTUP010000000
VERSION     DAJTUP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.290"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..263
                     /locus_tag="OO468_004190"
     CDS             <1..263
                     /locus_tag="OO468_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR6261060.1"
                     /translation="TDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVT
                     LHTDALHATRDEPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            275..646
                     /locus_tag="OO468_004191"
     CDS             275..646
                     /locus_tag="OO468_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6261061.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            633..>1103
                     /locus_tag="OO468_004192"
     CDS             633..>1103
                     /locus_tag="OO468_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6261062.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          256 a          252 c          339 g          256 t
ORIGIN      
        1 acactgacat ccgggataag tctgcccacc cggaaaatgc gctgaccgga catgaaaatg
       61 aggtgatatc agcgcagccg gagaccgtga ttcagaatat ggatgatctg gttacagtcg
      121 tggcactggt gacgctgcat accgatgcac ttcatgccac gcgggatgaa cctctggcat
      181 ttgtgccgcc gggaatggcg ttccgtgtct ttgccggtgt ggcagccgga atgacagaac
      241 gtggcctggc cagaatgcaa taacgggagg cagcgtggct gatttcgata atctgtttga
      301 tgttgcactt gatctcgcag acaaggccat tattcgcaat atggggatta gagcggtcat
      361 tacgtcaggc cggctaaaag ggatcatgat ttccggggtt tttgatgatc ctgaaaatat
      421 ttctctggtg gccggcggtg tgcgtattga agactcttta ccatccctgt ttgtgaaaac
      481 agcagatatt ttacggctgt gtcgcaatga ttcgctgatg attggtcgtg agtctttctg
      541 tgtggatcgt atcacccctg atgatggcgg atgtagttat atccggttgc ggcgtgaggg
      601 gctgccggga aacgtaaggg caggacgata ttatgaaggg gcttgagaat gccatccgca
      661 atctgaacag ccttgatacc cgtatggtgc cacaggccag cgcatgggcg ataaaccgtg
      721 tggcacagaa agcggtctcg gttgccaccc ggcaggttgc cgggaatacc gttgcgggcg
      781 ataaccaggt gaaagggatc cccctgaaac tggtacgtca gcgtgtccgg gtgtttaaag
      841 ccagtccgtc aggaaaaatg acggccagga tccgcgttaa ccggggcaat ccgcccgcca
      901 tcaaactgaa cacaacacgg cggcgtgctg gtgaaggact gagagtggga aaatactttt
      961 tccggggggc atttgttcag caactggcga atggccgctg gcatgttctg aggtgtcttc
     1021 ctgaagcgcg ttttgcaaca gggcatgacc atcagggcag gctaagaaaa aatcgtcttc
     1081 ctgtggaggt agtgaaaatc ccg
//
LOCUS       DAJTUP010000283         1084 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTUP010000283 DAJTUP010000000
VERSION     DAJTUP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.291"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..295
                     /locus_tag="OO468_004193"
                     /pseudo
     CDS             1..295
                     /locus_tag="OO468_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            662..>1084
                     /gene="ospC4"
                     /locus_tag="OO468_004194"
     CDS             662..>1084
                     /gene="ospC4"
                     /locus_tag="OO468_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR6261063.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNVLRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNF"
BASE COUNT          340 a          219 c          197 g          328 t
ORIGIN      
        1 acttccccgt gccccccggg tcatccggcc ggaagaggaa tgctgtccgg cctgtggtgg
       61 tgaactcagt ccactgggat gtgatgtgtc agagcagctg gagcttatca gcagcgcctt
      121 taaggttatc gaaaaacaac gccctaaact ggcctgtcgc cggtgcgacc atatcgtgca
      181 ggcaccggtg ccttcaaaac ccattgcacg cagttatgcc ggagcggggc ttctggccca
      241 tgttgttacc ggaaagtatg cagaccatct gccattatac cgccaaagtg atctactatt
      301 ccacacagct atttagctct ctttttgttg agctcccatg attctttcat ttattgttac
      361 taattatttt atgttatcat gatgttagaa cacgctctgc tctgtgtctc tattttatct
      421 atcatattct gaggattaac tacataacaa ggcgcaagat atatatacaa aaacaaataa
      481 taccgttgca ataattcctt ttagtaaagg aggtgtgatt aatttcaggg gatttcagag
      541 taaaaattgt acaacatatt aaaatatgtt catttcgtta taatacacac aaggaaatat
      601 taagttagat aatatattct aaataaccat agataaaaac gcacataatg gggaatattt
      661 tatgaaaata cctgaagcag taaatcatat taatgttcag aataatattg atcttgttga
      721 tggaaaaacg aatccgaata aagcaacaaa agcattacaa aaaaatgtat tacgcgtaac
      781 aaactcatct tcttctggca taagtgaaaa acatttagat cactgtgcta atactgtgaa
      841 aaacttttta aggaagtcta tagcggcgca aagttatagc aaaatgtttt ctcaagggac
      901 cagtttcaaa tcactaaatc tttctcttga agcgccatct ggagcacggt catcatttcg
      961 gtcacttgag catctcgata aggtgtccag gcactatatc tctgagataa tacaaaaagt
     1021 tcatcctctt tcttcagacg aacgtcactt actttctatt attattaatt ctaattttaa
     1081 cttc
//
LOCUS       DAJTUP010000284         1054 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTUP010000284 DAJTUP010000000
VERSION     DAJTUP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.292"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..136
                     /locus_tag="OO468_004195"
     CDS             <1..136
                     /locus_tag="OO468_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR6261064.1"
                     /translation="VGSQVRAGQPIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR
                     "
     gene            140..>1054
                     /locus_tag="OO468_004196"
     CDS             140..>1054
                     /locus_tag="OO468_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR6261065.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGW"
BASE COUNT          247 a          284 c          281 g          242 t
ORIGIN      
        1 cgttggttcg caggttcgcg cgggccagcc aattgcactg gtgggcagca gtggcggtca
       61 gggtcggcct tcactctatt tcgaaattcg ccgccagggt caggcggtca atccacagcc
      121 gtggttggga agataagttt tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct
      181 gttggcgctc tcctcccccg tacttgctgg caaacttgcc atcgtcattg atgattttgg
      241 gtatcgcccg cacaacgaaa accaggtgct ggcgatgcct tccgctatct ccgtcgctgt
      301 attacccgat tcaccgcacg ccagagagat ggcgaccaaa gcgcataaca gcggtcacga
      361 agtgttgatt catctcccga tggcaccgtt gagtaaacag ccgctggaga aaaatacgct
      421 acgcccggag atgagcagcg acgaaattga gcgcattatt cgtagtgcgg tcaataacgt
      481 gccctatgcc gtggggatca acaaccacat gggcagcaag atgacctcta acctgtttgg
      541 tatgcagaaa gtgatgcagg cgctggagcg ttacaatctt tacttcctcg acagcgtaac
      601 catcggtaat acccaggcga tgcgcgccgc gcaaggtact ggcgtgaagg tgatcaaacg
      661 gaaggtgttc ctcgacgatt cgcagaacga agcggacatc cgtgtgcaat ttaatcgcgc
      721 aattgatctg gcgcgtcgca acggttcaac cattgccatt gggcatcctc acccttcaac
      781 ggtacgcgtg ctacaacaga tggtttataa cctgccgcca gacattacgc tggtgaaagc
      841 cagcagcttg ctgaatgaac cgcaggttga tacttctaca ccgccgaaaa acgctgtgcc
      901 tgatgcaccg cgtaatccgt tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga
      961 acctgtttat gctaatcgct tctttgaagt attaagcgaa agcatcagcc agagcacgct
     1021 gatcgtttac ttccagcatc agtggcaagg ctgg
//
LOCUS       DAJTUP010000285         1038 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTUP010000285 DAJTUP010000000
VERSION     DAJTUP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.293"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..600
                     /locus_tag="OO468_004197"
     CDS             <1..600
                     /locus_tag="OO468_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6261066.1"
                     /translation="IRQYFSQVRTEKESIQALNHLRRVLHEVSPFAQEPVDCVLWVKA
                     DEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLVVDGFHRQLLGREA
                     DAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMSDIVRDLSRLGWTN
                     ERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            828..>1038
                     /locus_tag="OO468_004198"
     CDS             828..>1038
                     /locus_tag="OO468_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261067.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFS"
BASE COUNT          301 a          184 c          268 g          285 t
ORIGIN      
        1 attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc tctgaatcat
       61 ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga ctgtgtgttg
      121 tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat ggcactaggt
      181 gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc ggtggtggtg
      241 tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt actggggcgg
      301 gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat taatccggag
      361 agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc cagggggaaa
      421 caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg ctggacgaat
      481 gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct gaaacagatt
      541 agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac ggttcgttga
      601 tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa aaatgatacc
      661 gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta ggctttgata
      721 acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg cagatttaca
      781 aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg aaaaaacaga
      841 tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg aaaatattta
      901 aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc gaaccattcc
      961 acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata tttattaata
     1021 aattagttcc tttttctc
//
LOCUS       DAJTUP010000286         1028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTUP010000286 DAJTUP010000000
VERSION     DAJTUP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.294"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            68..220
                     /locus_tag="OO468_004199"
     CDS             68..220
                     /locus_tag="OO468_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR6261068.1"
                     /translation="MEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTM
                     SQQEVK"
     gene            220..570
                     /locus_tag="OO468_004200"
     CDS             220..570
                     /locus_tag="OO468_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6261069.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          344 a          229 c          245 g          210 t
ORIGIN      
        1 tgcttatgaa atcgcactcc tatctctgga agtagaacca gatgaacgcg aagcttatga
       61 attattcatg gaaaagcgtt ttggtgactt agtagatcgt cggagagcaa aaaacggcga
      121 taacgaatac atggcatggg atatgactct cggttggatc gtctggcagc aacgagctgg
      181 tatccatttc tcaacaatgt cacagcaaga ggtgaaataa tggagccata cagcctcaca
      241 ctcgatgagg cctgtcattt tctcaagata tccagaccga ctgccattaa ctggatacgc
      301 acagggcgtc ttcaggcaac acgcaaagat cccactaaga ataaatctcc ttacctcaca
      361 acacgacaag cctgcattgc ggctcttcag tctccgctgc atactgtcca ggtgagcgcg
      421 ggtgatggca taacagagga aagaaaatgt cactcttccg cagaggtgaa atatggtacg
      481 ccagtttcac attgccgaac ggtaaaagat ttaaacagtc tcttggaaca aaggacaaaa
      541 ggcaggcgac agaactccat gacaagctaa aggctgaagc atggcgggtc agcaaacttg
      601 gtgaaatacc tgatataacg ttcgaggaag cgtgtgtcag gtggcttgaa gagaaagcac
      661 ataaaaaatc actggacgat gacaaaagcc ggatcggatt ctggcttcaa catttcgcag
      721 gaatacaact aagagacatt actgaatcaa aaatttattc agcaatgcag aaaatgacga
      781 accggcgtca tgaggaaaac tggaaactca gggcagaatc atgcagaaaa aaagggaaac
      841 ctgttccaga atacacgcca aaaccagcgt ccgttgcaac gaaggctacg catctttcat
      901 ttataaaggc cctactaaga gccgcagagc gtgaatggaa aatgctggat aaggcaccaa
      961 ttattaaagt gcctcaacca aagaataaac ggatccgctg gctggagccc catgaagcac
     1021 aaaggctg
//
LOCUS       DAJTUP010000287         1016 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTUP010000287 DAJTUP010000000
VERSION     DAJTUP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.295"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(214..852)
                     /gene="ospI"
                     /locus_tag="OO468_004201"
     CDS             complement(214..852)
                     /gene="ospI"
                     /locus_tag="OO468_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR6261070.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          305 a          247 c          143 g          321 t
ORIGIN      
        1 caccgtcatg ccaggctcca ttgtctgctg aataatggca attttttcct gaggagtttt
       61 acgtttacga agctccagcc ccagcaaagt tccagtcatc tcaaaattag aactagtgtt
      121 agacatatat ccaagcctat ctcttatttt aagatacatc cactgtctgg cgtttcaagg
      181 ggccggctca aatctatgta caggctctca gcatcagcaa agcctcttac ttttccaact
      241 actactgtta gcatagtgat caaattcctg atcagataca tgaattacct gaaccgaact
      301 attttcatct acaaatgcat ctggcgattt ttctacagga tcataaagat taaatccctt
      361 tcctgtttga acatccaaaa acataatttt ttcaccatcg ttaaatccag ctatccaatg
      421 agtggcctgt tcaatagaaa tcattactac acttcctttt gaagtatttt gtaaaatatt
      481 ttctgcaata cgctcacgaa atttactata agtttcatta ggatgtgaca ctatgccttc
      541 actatcaatt tgctctaaat gcctatgaaa aaatccggtc atatactctg atatttcatt
      601 aggtacggga gcctctctta ctccataccc taacatagcc atacaagcat gaagcgcaca
      661 ccctgaacag ttaccatccc caccagacgg atttatcatt ttctgtaaag tcgctgccaa
      721 ttcagaatct ttatccatat caccaagact tttagagttt aaagattcca gcaatttttt
      781 aacatttaca tttccctcct ctgtatgtgc ctcatatcca gcagttccct gtaacgacac
      841 cccattaatc atattgtatc ctgacagtat atatactcac tatattttct tcataacaaa
      901 tatccctctg atatgacaac aaaaaacgga gccggattcc tgtttttgtg aactgcagct
      961 attcgtgtct aaaaagtgat ccacttacca ccaacggcct aatatataca gaacgg
//
LOCUS       DAJTUP010000288          988 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTUP010000288 DAJTUP010000000
VERSION     DAJTUP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.296"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OO468_004202"
     CDS             complement(10..813)
                     /gene="dkgB"
                     /locus_tag="OO468_004202"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR6261071.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          261 a          222 c          224 g          281 t
ORIGIN      
        1 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
       61 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
      121 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
      181 agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa tacgagcaat
      241 aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga tatgaatacc
      301 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
      361 gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca atgggatcgt
      421 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
      481 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
      541 aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca atttgtcttt
      601 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
      661 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
      721 gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt ctttcagacg
      781 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
      841 ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa acgcagagga
      901 tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag cgttaactcg
      961 aggcttctta ataagtggcg gaacggac
//
LOCUS       DAJTUP010000289          980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTUP010000289 DAJTUP010000000
VERSION     DAJTUP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.297"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..475)
                     /gene="speB"
                     /locus_tag="OO468_004203"
     CDS             complement(<1..475)
                     /gene="speB"
                     /locus_tag="OO468_004203"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR6261072.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANG"
     gene            complement(611..>980)
                     /locus_tag="OO468_004204"
     CDS             complement(611..>980)
                     /locus_tag="OO468_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261073.1"
                     /translation="YQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVNAGD
                     SLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPP
                     CSGQAFPADNNGYAPPPCRK"
BASE COUNT          244 a          262 c          257 g          217 t
ORIGIN      
        1 aaccgttcgc ataggtatcg gtgtgggcgt caaagtgtac cagcgccatt ttgccgaaat
       61 gcttcgcatg agcacgcagc agcggcagcg taacaaagtg gtcaccaccg aaggagagca
      121 tacgcttacc ggcagccagc agcttctcgg cgtgcgcctg cagcttttcg ctcatctcac
      181 gggcatcgcc aaaggcatat accagatcgc cgcagtccac gacgttcaga cgctcacgca
      241 tgtcgaaatt ccacgggaag cggttgtgtt cccaggccag attcgtcgaa acctgacgga
      301 tcgctgccgg accgtggcga ccacccgcac gaccagaagt ggccatatcg aacggcacgc
      361 cagtaatcac ccagtctgca tcgctgtcat acggctggaa gttcatcggc aggcgtaaaa
      421 aaccaaaggc attggaaacc agtgagttat cgtattgatg acctaaggtg ctcatggatg
      481 gactcctatt acaaagtcga tataaaaaaa cccttccgcg tcgttaggcc cgacgaggaa
      541 gggttggatt tgcgaaacac aagtttgtgc taattatcgc cttaaatggg cgaggcatca
      601 agcgaggttg ctatttccga cacggtggcg gcgcatagcc attgttatct gccggaaagg
      661 cttgtccaga gcacggcggc agcgcgtttt gcgccgggcc ttgtaattca aaggcggcaa
      721 agccgattac cccggtatta cgcacatcgc cttgcgccga attggctgcg taggaatcag
      781 caacgttggc gaactgaaaa gccgcttcag tatgtttatc tttgcggaat cctttaatca
      841 ctaacgaatc tccggcattc acaatatagc cgttgttatt cagcgaacct tgcttgccgt
      901 tcagcacatc caggccatca gccgtagcaa cgatctcgta gttggtgtta cgactgtaat
      961 tacggacata taattgataa
//
LOCUS       DAJTUP010000290          980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTUP010000290 DAJTUP010000000
VERSION     DAJTUP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.298"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..411)
                     /locus_tag="OO468_004205"
                     /pseudo
     CDS             complement(1..411)
                     /locus_tag="OO468_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(459..824)
                     /locus_tag="OO468_004206"
     CDS             complement(459..824)
                     /locus_tag="OO468_004206"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261074.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
BASE COUNT          235 a          285 c          214 g          246 t
ORIGIN      
        1 cgagccagcg gctcatgcat cataacctgc tctgccagac ggtaacccct cttcagcccc
       61 gtaaaccgca tctgacctcc gcgcagctcg cacttcagcg ggtcaacctt cagacactgc
      121 tgatacatcc ctctccttgt gatttgcctc gccgtttttc tcactgtctc cgttatggcg
      181 tacaccacat cttccagtaa tcgccgcttc accggactca ggaccccgta tcaccatccg
      241 gaacccctta tccgccacat gccaggagaa cctttccata aactcgtcgc cactcatcac
      301 aaggttttct tctcgttttg tccggtggct gttgtaacgc aggccaattt catcctgacc
      361 ggcataatgt tccaggcggc tcatctgcac cggctgcttc tccagccagt taagccatcg
      421 gtttccctgc tgaaaaatat cggcaaaacg gggaagcatc agaaaggcgg aataacgcca
      481 tccggccagt gaaccgtacc gcattcaggg caatacatgc cgtcggcgtt gataccggca
      541 ggaattgtcg caaattcccg ctccgtgcag cgggcaattt ccggataccc ttcgtcatca
      601 acacgtacaa accagaagac cagttttttg tttccggcat ccacaaagaa cggaatattc
      661 aggtctgcgc aacactcaac ggcatcgtca aaattatcaa agcgcagaac ctcagcttct
      721 tcttcgtcaa aaaaatcatc ttcgtgaagt tttacgacat agcggaggag tttgcttctt
      781 tgaggtgcag gttttcgttt gcgtggttta gctgaacggg ccatataact acctgaaaga
      841 caatgatatt gtctgttttt ataacggtaa tcacaggcca tgacaagccg cagccgtcag
      901 gccgcctact cggggcggtt tagaacgctt atttagtacc agatcttact tataataaag
      961 gggcttggca gtttggataa
//
LOCUS       DAJTUP010000291          937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTUP010000291 DAJTUP010000000
VERSION     DAJTUP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.299"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(191..856)
                     /locus_tag="OO468_004207"
     CDS             complement(191..856)
                     /locus_tag="OO468_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR6261075.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          242 a          231 c          211 g          253 t
ORIGIN      
        1 aacctacatg tgcaaagcat gaaattggcc tcagattaat gttaagcgtc ttgccggacg
       61 cgtaatgtta actggggctt ttctctatct gccttttggt gttcatgcct gaagcagata
      121 gcctcaagca cccgcagtca ttctacttac ctcgccaata tgaaatcaat cagaaaggtg
      181 ccccataaaa tcactccttc tcttctttac cgtagtggag ttgaccaatt ttgataagag
      241 ggcgtccctg agatttgcgg tgtagattgg tatcgcgcag agaatacaca cagccacaat
      301 attcctgctg atagaatttt tcgcgcttgc tgatttcaat catacgggac gagccgccct
      361 gcttgcgcca gttataatcc cagtacacca tgcccggata atgcgcgacg gctcgccgcc
      421 cacagtcgtt aacctgctgc atatttttcc agcgtgaaat gcccagtgaa ctgctgatca
      481 cactgaaacc attttcagcg gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca
      541 tggtgcaacg gatccctcgt tcgggctccc attccattcc tttggctcgt tcaaaccagt
      601 tgtctgtgtc gtaatcagca tcaataaacg gcacgccgtg ttgttcagca aagcgaatat
      661 tctcatcctt acgaattaaa tactctttct gaggatgaat gttcgggttg tagaaaaaga
      721 tggtgtagtc gatttccgag gcctgaagcg cctccatcac ctcaccggaa catggagcac
      781 agcaagagtg cagtagtagt ttgtttgccc cgtttgggag ctccaatttg ggccgtttga
      841 aatcagcaac tgtcataaat atgtttattg aggtcataaa aatatcaaag agtgtagcat
      901 tagaacgggg ctatcggaaa cagatgtgtg actgctc
//
LOCUS       DAJTUP010000292          931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTUP010000292 DAJTUP010000000
VERSION     DAJTUP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.300"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..269
                     /locus_tag="OO468_004208"
     CDS             <1..269
                     /locus_tag="OO468_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6261076.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OO468_004209"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OO468_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR6261077.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>931
                     /locus_tag="OO468_004210"
     CDS             613..>931
                     /locus_tag="OO468_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR6261078.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          253 a          238 c          258 g          182 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac c
//
LOCUS       DAJTUP010000293          882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTUP010000293 DAJTUP010000000
VERSION     DAJTUP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.301"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..503
                     /locus_tag="OO468_004211"
     CDS             <1..503
                     /locus_tag="OO468_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR6261079.1"
                     /translation="EQYQMTKRYSVTWLFSEDGKNWYEELKNFASDTIKIAYTGDGRV
                     VWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALL
                     AQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPA
                     SPEGSS"
     gene            527..>882
                     /locus_tag="OO468_004212"
     CDS             527..>882
                     /locus_tag="OO468_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR6261080.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMAG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGA"
BASE COUNT          243 a          194 c          246 g          199 t
ORIGIN      
        1 cagaacaata tcagatgaca aaacgatatt cggtcacctg gcttttttca gaagacggaa
       61 aaaactggta tgaagagctg aagaatttcg ccagcgacac aataaaaata gcttacaccg
      121 gagatggtcg cgtggtgtgg gtcggtaagg atgtgacagg catcgagcca cgcaatgcca
      181 gcgttatcga agttccggat attaccgcta accgacggat taccgcgccg ggttactggt
      241 tttaccgcaa tgatgaattt gtctttgact acagactcaa agcggaagat gagcgtgatg
      301 cccttctggc tcaggtcagt gcccggacag gggaatggga agaagacctg ctgctggggt
      361 taatcagcga cgaagataaa gaaaagctga aagcctgtcg tatttacgcg aaatcgctgc
      421 aggcgatgga tttcagcacc atcactgata aatcctcata caacgccatt gaatggcccg
      481 cctctccgga aggttcttcc tgatttaatt tatcgcgaga aaaacaatgt ctgtagtgat
      541 atcaggtgcg ctgactgatg gcgcaggtat ccccatgtcc ggataccata ttattctgaa
      601 atcccgggta aacaccccgg aagtggtgat gaacactgtt gctgatgtga tggcaggaaa
      661 cgatggtgaa tactgtttcc atgcgcggac tggaaaatat ggtgtgtatc tgaaacagga
      721 ctggcgcaac gagtacaacg ttggcgacat tgctgtatat gaggactcaa agcccggcac
      781 gctgaatgac tttctgattg ctcctgatga gggcgacctg aaaccggatg tcgtcaaacg
      841 ctttgaggaa atggtggcgc aggcgcagca gagcgccggg gc
//
LOCUS       DAJTUP010000294          877 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTUP010000294 DAJTUP010000000
VERSION     DAJTUP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.302"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            20..>877
                     /locus_tag="OO468_004213"
     CDS             20..>877
                     /locus_tag="OO468_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR6261081.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPYLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREE"
BASE COUNT          202 a          228 c          254 g          193 t
ORIGIN      
        1 gagtcgcccc gcccttctga tgctcccccg ttttgccgat atttttcagc agggaaaccg
       61 ctggcttaac tggctggaga aacaaccgga aggggcagtc cgtccggtgg taattgaatc
      121 agtgacaaaa atcatggcct gcgggaccac actgatgggc tacacgcagt ggtgttgttc
      181 atcaccggac tgttgccata caaaaaaggt ctgcttccgg tgtaaaagcc gctcctgccc
      241 gcactgcggg gtgaaggctg gcgcacagtg gatacagtat ctgctgagcc tggtccccta
      301 ctgcccgtgg cagcatattg tgttcacact tccctgccag tactggtccc tggtgttcca
      361 caaccggtgg ttactggcag agatgagccg cattgcagcg gatgtgatac tggaaatctg
      421 ccatcaggca gatgtggagc cggggatatt cacggtgatc cacacatggg ggcgtgacca
      481 gcagtggcat ccgcatatcc atttatcgac aactgccggt ggtgtgacgt cgggccacac
      541 ctggaaaaat cttcattttt acgcccgtaa ggtgatgagc atgtggcgct accggataac
      601 acggttgctg tcacggaaat acccttacct ggtgatgccg gatgcgctgg cagcagaagg
      661 aagcagtaaa cgggaatgga atcgcttcct ggacacgcat taccgccgtg gctggaatgt
      721 caacgtatcc cgggtaatgg ataatgccac ccatgtggcg gtgtactttg gctcttacct
      781 gaaaaagcca ccggtgccga tgagtcgtct ggagcattat gccggtcagg atgaaatcgg
      841 tctgcgttac aacagtcacc gaacaaaacg ggaagaa
//
LOCUS       DAJTUP010000295          870 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTUP010000295 DAJTUP010000000
VERSION     DAJTUP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.303"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..287
                     /locus_tag="OO468_004214"
     CDS             <1..287
                     /locus_tag="OO468_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCR6261082.1"
                     /translation="KMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPH
                     AAKLAMWQHLENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            632..>870
                     /gene="yqjA"
                     /locus_tag="OO468_004215"
     CDS             632..>870
                     /gene="yqjA"
                     /locus_tag="OO468_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HCR6261083.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAA"
BASE COUNT          235 a          207 c          193 g          235 t
ORIGIN      
        1 aaaaaatgtg gacccagcgt agccataacc cgtactggaa aaaactacac gaacacattg
       61 attcccgtac cgttgataac tggtgtgatg accacgatca aattctcaag gcgttaattc
      121 gcaaagatcc tcatgctgct aagctggcaa tgtggcagca tctggaaaac accaaaatca
      181 tgttatttaa cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctgttcgccg
      241 aaaacccggt agtgcatctc gatacggcca ccagcggcag caaatgaaat tcctgtccga
      301 caggcgctgc aatgcgcctg tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct
      361 gtgtaaatcc tctcgccacc ctcccctgca atcagcaaaa tcagactcca cggacatgta
      421 attttgataa cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgctt
      481 ttgaatgctt tcttatctca cgttttaaca gggaatagtt caggctgtgt tgatgtatca
      541 aacccgcaga acataccaaa acagcaataa cattgcggta gtgcatcttt taaaaccagc
      601 gtggcgttaa ccgattcacc aggaataatg aatggaactt ttgacccaat tgctgcaagc
      661 cctgtgggcg caggattttg aaaccctggc caatccatcg atgattggca tgttgtattt
      721 tgtcttgttt gtaattttgt tccttgaaaa tggcttgctt ccggcggcct ttttaccggg
      781 cgacagttta ctggtattgg tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca
      841 aacgattctg ctgctgaccg ttgccgccag
//
LOCUS       DAJTUP010000296          867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTUP010000296 DAJTUP010000000
VERSION     DAJTUP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.304"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            492..867
                     /locus_tag="OO468_004216"
                     /pseudo
     CDS             492..867
                     /locus_tag="OO468_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          297 a          189 c          133 g          248 t
ORIGIN      
        1 gaaagatcac atacaaagaa aaatttgttt attaacagtt cattgcgaaa ccatctagcc
       61 aacaaatgct aaattggttc gcaatcaggt acacaaatga aagacattaa cctctcacct
      121 tccacccggt tgaacgatgt taacactaaa aaaagggaga ttgtaccttt ccgtttcaca
      181 ttgattcgat tcgcaattcg tttgtagtga gtacaccagc aaaacgctta aagaaccact
      241 gataacatca cggtgcgtct gaaaggttta ctgcgagcgg atttacagca gcataaactg
      301 aatccagcaa agttcgttgc gtgatttggc ggtagtattt taaacgctca tgagattaat
      361 gacgaagtgg tcatatcaca atgataaaag tgacacaatt cttataacaa tttttcgtgc
      421 acatttcgtt ctggcgataa taattaatca tcatcctcaa aacctccaga tatatataag
      481 gcgaatagat tatgagtcaa aacgatatca ttatcagaac tcattataag tctcctcata
      541 gattgcacat cgatagcgac ataccaacac cttcatcaga gcctattaat aaatttgcgc
      601 gccagctcat caccctactt gatacctctg acttaagttc gatgctgtca tactgtgtta
      661 ctcaggaatt taccgcaaac tgtcgaaaat atcacaaaat tgttattcca ctgccctttt
      721 taccattaac tttgccactt cacccatcca tgcagaaaat atactcatta cattacacta
      781 taaaaaagaa atcatttcct tattactaga aaccacgcct attaaagcta accatttgcg
      841 aagcatactg gattatattg aacagga
//
LOCUS       DAJTUP010000297          861 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTUP010000297 DAJTUP010000000
VERSION     DAJTUP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.305"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..204
                     /locus_tag="OO468_004217"
     CDS             <1..204
                     /locus_tag="OO468_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR6261084.1"
                     /translation="LQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAP
                     QTFIGLLKGKNFGKVVIRVAGDD"
     gene            402..>861
                     /gene="mcbR"
                     /locus_tag="OO468_004218"
     CDS             402..>861
                     /gene="mcbR"
                     /locus_tag="OO468_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR6261085.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCE"
BASE COUNT          260 a          174 c          213 g          214 t
ORIGIN      
        1 ttgcaagggt ttattatcgc tcaggattat ggtcaccgca tccatgagtt tcagaaggag
       61 atggggcaat gggtgaaaga ggataaaatc cactaccgcg aagatattac tgatggttta
      121 gagaatgcgc cacagacgtt tatcggcctg ctgaagggta aaaacttcgg caaagtggtg
      181 atccgcgtcg cgggtgatga ttaaagtttc aacggcggcg taagccgcca taaaaatcca
      241 ggcaaaaagc ttattttcag ctttaattaa ctaaccacgt cattgttctg ctgctaataa
      301 caacaaaggt gggtggttta tgattattca ctttaataca ctaggtgaac tccttctgcc
      361 atgcaggcag ggttggacag aaaacacgcg aaggaattaa aatgcctgga acgggaaaaa
      421 tgaaacatgt cagtttgact ctgcaggttg agaacgacct gaaacatcag cttagtattg
      481 gcgcattaaa acctggcgca cgcctgatta ctaaaaatct ggcggagcaa ttaggtatga
      541 gtattacacc tgtgcgtgaa gcattattac gtctggtttc ggtgaatgcg ctttctgtcg
      601 cacctgcaca agcatttaca gtaccggaag tgaggcaaag tcagttagac gaaatcaatc
      661 ggatccgcta cgaactggaa ttaatggcag ttgccctggc tgttgaaaac ctctcctcgc
      721 aagacatagc agaacttcag gaattgctcg ggaaattaca gcaggcgcaa gaaaagggcg
      781 acatggaaca aatcattaat gtgaacaggc tatttcgctt agcgatttat catcgctcaa
      841 atatgcccat cctgtgtgag a
//
LOCUS       DAJTUP010000298          858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTUP010000298 DAJTUP010000000
VERSION     DAJTUP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.306"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(31..90)
                     /locus_tag="OO468_004219"
     CDS             complement(31..90)
                     /locus_tag="OO468_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR6261086.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(329..>858)
                     /locus_tag="OO468_004220"
     CDS             complement(329..>858)
                     /locus_tag="OO468_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261087.1"
                     /translation="QVALIAAAQDAETKAVELTVRAKAEKEAAEMQAAAIVELAEATH
                     KKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEPMKSIDGIKIIQ
                     VDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNEIGVSGGSLAAL
                     TSPLISTTPVAENVK"
BASE COUNT          183 a          232 c          204 g          239 t
ORIGIN      
        1 gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta cgagtaacaa
       61 cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg ccccttcctc
      121 gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg agcgccggac
      181 ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg caaaacgttg
      241 caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc cccggctttt
      301 tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg ggagttgttg
      361 agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt tcattgagca
      421 aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat gcttgttccg
      481 ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg ccactattca
      541 gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca acggattttt
      601 ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg gtttgttcat
      661 cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc gccagaccct
      721 ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct gcggcttctt
      781 tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc tgcgcggcag
      841 cgattagggc aacttgtt
//
LOCUS       DAJTUP010000299          851 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTUP010000299 DAJTUP010000000
VERSION     DAJTUP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.307"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..725
                     /gene="apbC"
                     /locus_tag="OO468_004221"
     CDS             <1..725
                     /gene="apbC"
                     /locus_tag="OO468_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR6261088.1"
                     /translation="LALAAEGAKVGILDADIYGPSIPTMLGAENQRPTSPDGTHMAPI
                     MSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDYLVLDMPPGTGDIQ
                     LTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMSVHICSNCGHH
                     EPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRPESEFTAIYRQLAD
                     RVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          196 a          215 c          231 g          209 t
ORIGIN      
        1 cgctggcact ggcggcggaa ggggcgaaag tcggcattct ggacgccgat atctacggcc
       61 catcaattcc caccatgctg ggcgcagaaa atcagcgtcc aacttcgccg gacggtaccc
      121 acatggcacc gattatgtct catggcctgg caaccaattc aattggttat ctggtcactg
      181 acgacaatgc gatggtgtgg cgtggaccga tggcgggcaa ggcgttaatg cagatgttgc
      241 aggaaacctt gtggccggat ctcgactatc tggtgcttga tatgccgcca ggcaccggtg
      301 acattcaatt gacgctggcg caaaacattc cagtaactgg tgcagtcgtg gtcaccaccc
      361 cgcaggacat cgcgttgatc gatgcgaaaa aaggcattgt gatgttcgag aaagtcgagg
      421 ttccggtgct cggtattgtc gagaacatga gtgtgcatat ttgcagtaac tgtggtcacc
      481 atgaaccgat tttcggtacc ggtggtgcag agaaactggc tgaaaaatac catacacagc
      541 tgctggggca gatgccgtta cacatctcct tacgtgagga tctcgataaa ggcacgccaa
      601 cggttatcag ccgtccggag agcgaattta ccgctatcta ccgccagctg gctgaccgcg
      661 ttgcagctca gctctactgg cagggtgaag tcattcctgg cgagatttcc ttccgcgcgg
      721 tctaacgcct attaaccatt ctggttattc ttcatacacc gcagagatgt tatattgatg
      781 cggtgtattt gtttttgccg cattaatgaa cacttattta atactgcgaa tattctgcgg
      841 accttcgtcc g
//
LOCUS       DAJTUP010000300          848 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTUP010000300 DAJTUP010000000
VERSION     DAJTUP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.308"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..509
                     /locus_tag="OO468_004222"
     CDS             <1..509
                     /locus_tag="OO468_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR6261089.1"
                     /translation="QLWWDKDADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTAWPH
                     DGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFKVFN
                     TCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKIPAP
                     IRPIARRT"
     gene            510..>848
                     /locus_tag="OO468_004223"
     CDS             510..>848
                     /locus_tag="OO468_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6261090.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPVVRKLVAEMRRNPIDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVG"
BASE COUNT          233 a          204 c          232 g          179 t
ORIGIN      
        1 ttcagctttg gtgggacaag gacgcagaca caatatacgt ttcacgcgtg tggaaggcga
       61 aagaaaaaac agccgttcag gcgtggggag ccgttaaatc atgggcgcat aaagtgccaa
      121 ctgcatggcc tcatgacgga aaccagcacg agaagggcgg cggtgagcag ctcaaagggc
      181 agtatgcaga tgctggtttt atgatgttgc aggagcatgc gacatggcct gatggcggta
      241 atgctgtgga gcctggcatc actgaattgc gcgacatgat gctcgatggt cgcttcaaag
      301 tattcaacac ctgtgagcca ttctttgagg agttccgcct ctatcaccgt gatgaaaacg
      361 ggaaaatcgt caagcttaac gacgacgttc tctcagccgt tcgctatgca tacatgatgc
      421 gccgcttcgc caaaatgatg cgcgacatca aaaaaccaaa agagaaaaag ataccagccc
      481 caatcaggcc catcgcacgg agaacttaaa tggccgacga aaacagactc aattccattc
      541 tgtgtaagtt tgacgcggac tggatggcga gcgatgaagc cagaaccgag gcgacaaatg
      601 acctgtattt tagccgagtg tcgcaatggg atgactggct atcaaactac actaccctgc
      661 aatatcgcgg acaattcgat gttgttcgcc cggtggtcag gaaactggtc gcagagatgc
      721 gccggaaccc tatcgacgtt ctcttccgac ccaaagacgg cgctaatcct gatgctgccg
      781 atgtgttgat gggaatgtat cgtactgata tgcgccataa cacggcaaag attgccgtta
      841 acgttggc
//
LOCUS       DAJTUP010000301          839 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTUP010000301 DAJTUP010000000
VERSION     DAJTUP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.309"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>839)
                     /locus_tag="OO468_004224"
     CDS             complement(<1..>839)
                     /locus_tag="OO468_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261091.1"
                     /translation="TDSWFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCF
                     GSSTIEYFSLTGATTVGAALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPS
                     VYIIGSGQVSPIASASIEKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDA
                     SSSANGPQWCVLKTGLYDDVYRAIDFIYEGNQITCGDKLESVTGKLQFDISSQYDKQQ
                     EHLLFTPLFKADNARVFDLEVESSTGVAQYADRLFLSATTDGINYGCEQMIEQNEPFV
                     YDKR"
BASE COUNT          208 a          213 c          218 g          200 t
ORIGIN      
        1 cgtttgtcgt aaacgaacgg ttcattctgc tcaatcatct gctcacaccc gtaattgatg
       61 ccgtcagtgg ttgcagagag aaaaaggcgg tcagcatact gcgcaacgcc agttgacgat
      121 tcaacctcaa ggtcgaacac tctggcgtta tccgctttga acagtggagt aaacagcagg
      181 tgttcctgtt gcttgtcgta ctggctgctg atgtcgaatt gcaatttccc ggtcacggac
      241 tccagcttat cgccgcacgt tatttgattg ccttcgtaaa tgaagtcgat agcgcggtac
      301 acatcgtcat acaggcctgt tttcagcaca caccattgcg gaccattggc gcttgaagat
      361 gcgtcgtaca ccagtacatg gcgcggaagg tggataatca gcaactcatg cgcatcaaac
      421 cgcagcgatt ccatcacgcc atcagccagt tcatcagcag tgtaggagcg gaggattttc
      481 tcaatgctcg cgctggcgat tggtgacacc tgaccggagc cgatgatgta tacagacggc
      541 gcacctgttg ccggattgct gatgaacgca taagaatcag cgaatggtgt tttgcagtaa
      601 gtcccggcaa tacctttctg caccatcagc gatggctgtg cgacatacaa agcggcacca
      661 acggtggttg cacctgtcag ggaaaaatat tcaatcgtcg atgagccaaa gcagacgatg
      721 aagtctcgcc atgtacctat gccgatgatg ccgtccggct gagactcggc acgatattgt
      781 gcactgtagc ggtcaggatg cgattcatct tcaaggtcag tgataaacca tgaatcagt
//
LOCUS       DAJTUP010000302          812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTUP010000302 DAJTUP010000000
VERSION     DAJTUP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.310"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..699)
                     /locus_tag="OO468_004225"
     CDS             complement(<1..699)
                     /locus_tag="OO468_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6261092.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLN"
BASE COUNT          188 a          179 c          201 g          244 t
ORIGIN      
        1 attgagaccc agatggtcga atatttcgat taacgcagcg tgtgacgcct tgttttccct
       61 taaggttgca ggcttccagt tctgatagtt ctccgcttcg tattgttcaa agaggctcgc
      121 tatggtaatg ctaggcgtaa cttctggttt tacttctgga gcattaacag atagggacac
      181 agagtctgct tggttatctt gcctatcaat atcattaaaa agatcgacgt aatctatcag
      241 aggctgactg tctcccgctt cgatacgcct catacaggct tttagcacct tcagggcctc
      301 attgatgtaa cgatgctggt caatgcttag cggctcacta gccagagcat cggtaaggct
      361 ctcaccaagt tctccgtatt ggtcccggta gatatccctc atgtcaggct caaagagatc
      421 tgaacggccc atagacagtt cacactcagc gatgtcctta aggtgttccc tcatttcctc
      481 ataggtagcg ttgggtctgt ctgcgtgtat tgccttgagt gctgccttta tgtgccttga
      541 gtaagccata gcggtgcgtc tatccttggt ccgtaatgat attgaggtca ttctatcatt
      601 gtttgattgt ctgaccctaa gacggaagta gtagacgccg ttacgctggt agagatagta
      661 gactgctgga agtttgtgac tactggatag ctgaatcata tgtctgcatg ccctgagagg
      721 gttacacagt gggttacagt aagtaactct gcaaggaggc ttaaagccgt atcccttagg
      781 attcagtagg ttggagtact ttaagaaagg tc
//
LOCUS       DAJTUP010000303          806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTUP010000303 DAJTUP010000000
VERSION     DAJTUP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.311"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..444
                     /locus_tag="OO468_004226"
     CDS             <1..444
                     /locus_tag="OO468_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR6261093.1"
                     /translation="ASNATLTDVEPQSMEDGVNDLEMMMAEWLGGDVSPGINVGYIFA
                     DADVAPEPGDEHGLSNNAINAVIFNLACRIAPDYALEAPAKLITTARYGKERLVKLSA
                     MDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEPCDNGGE"
     gene            445..>806
                     /locus_tag="OO468_004227"
     CDS             445..>806
                     /locus_tag="OO468_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261094.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTV"
BASE COUNT          211 a          184 c          223 g          188 t
ORIGIN      
        1 gcatcaaatg ccacgttaac cgatgtagaa ccgcagtcta tggaagacgg cgtcaacgac
       61 cttgaaatga tgatggctga atggcttggc ggtgatgtgt cacctggtat caacgttggc
      121 tacattttcg ctgatgcaga tgtcgctcca gaaccgggcg atgagcacgg tttatcaaat
      181 aacgctatca atgccgtcat tttcaacctt gcctgccgca ttgctccaga ttatgcgctg
      241 gaagcgccag caaaacttat aaccactgcc agatacggga aagagcgact cgtcaaactg
      301 tctgcaatgg acagagcaaa agccgctaaa tgtaagtccg gttatccaaa ccgtatgcct
      361 gttggtagcg gtaaccagtt ggcgaagtgg aatggttgga attacttcca ccggaaggaa
      421 ccttgcgata acgggggcga ataaatgccg attcagcaac ttccgcttat gaaaggtgtc
      481 ggcaaagatt tccgaaacgc cgactacatc gactatctgc cagtgaatat gctggctaca
      541 cccaaagaaa tactcaacag cagcggatat cttcgctcat tcccgggcat tgccaaacgt
      601 tctgatgtga atggtgtatc gcgcggtgtc gagtacaaca tggcgcagaa tgctgtttat
      661 cgcgtttgcg gtggcaagtt gtataaggga gaaagcgagg tcggtgatgt tgccggaagt
      721 ggtcgcgtat caatggcgca tggtcggaca tcacaggcgg taggcgttaa tggtcaactg
      781 gtcgagtatc gctatgatgg cacggt
//
LOCUS       DAJTUP010000304          803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTUP010000304 DAJTUP010000000
VERSION     DAJTUP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.312"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..527)
                     /locus_tag="OO468_004228"
                     /pseudo
     CDS             complement(1..527)
                     /locus_tag="OO468_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            535..759
                     /gene="tnpA"
                     /locus_tag="OO468_004229"
                     /pseudo
     CDS             535..759
                     /gene="tnpA"
                     /locus_tag="OO468_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          205 a          194 c          183 g          221 t
ORIGIN      
        1 gaagggtgag ccggagtgga tacttcactt tctgtctgaa tactgatgta ccacgtaccg
       61 caggactggc tgacagtgac atttttcaca acacccgtga cctgacggct attccggtag
      121 cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag cttaacgccc
      181 tgcgggtagc ggaatgcatc attctgtcac cgctttttga atcggggaaa agcagcccgc
      241 ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg caatggctgt
      301 gagggagaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac cagccaggaa
      361 gccattttcc cgtaagggat gtatttatta ccggccttat gattctcatt ctgacgtgcc
      421 agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acactcctgt
      481 tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg tcaaatgata
      541 ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt tttcctgatg
      601 catgttcacc tagtctttgt caccagatac cgacgccaga tttttgacca tgacgcgaca
      661 gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga actggttgaa
      721 atggatggcg aaccagatca cgtccatttg ttaattaact aacctcccaa actggcgata
      781 tccagtctgg taaacagcct caa
//
LOCUS       DAJTUP010000305          783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTUP010000305 DAJTUP010000000
VERSION     DAJTUP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.313"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            8..>783
                     /gene="curA"
                     /locus_tag="OO468_004230"
     CDS             8..>783
                     /gene="curA"
                     /locus_tag="OO468_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR6261095.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELP"
BASE COUNT          173 a          173 c          251 g          186 t
ORIGIN      
        1 gagcattatg gggcaacaaa agcagcgtaa tcgacgttgg gttctggcct cgcgtccaca
       61 tggcgcaccg gttccggaga atttccgtct tgaagaagat gatgtcgcca caccgggtga
      121 aggacaggtg ttactgcgca cagtttattt gtccctggac ccgtatatgc gtggacgtat
      181 gagcgatgag ccatcttatt caccgccggt tgatattggc ggcgtgatgg tcggcggtac
      241 ggtgagccgt gtcgtggagt cgaatcatcc tgattatcag cctggcgact gggtgctggg
      301 ctacagtgga tggcaggact atgacatatc cagtggtgat gacctggtga aacttggcga
      361 tcatccgcaa aatccatcgt ggtcgctggg tgtgctgggg atgccaggct ttaccgctta
      421 tatggggctg ctggatatcg gtcagcctaa agagggcgaa acgttggtgg tagctgcggc
      481 gacagaacca gtgggggcga cggtggggca aatcggcaaa cttaaaggtt gcagagtggt
      541 gggggtagcc ggtggcgcgg aaaaatgccg ccatgctacc gaggtgttag gcttcgatgt
      601 ttgccttgat caccacgcgg acgattttgc cgaacaactg gcgaaagcgt gcccaaaagg
      661 tattgatatc tattatgaaa acgtgggcgg taaggtattt gatgcagtgc taccattact
      721 taatacatct gcgcgcattc ccgtttgtgg cttagtgagc agctataacg ctacagagct
      781 acc
//
LOCUS       DAJTUP010000306          773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTUP010000306 DAJTUP010000000
VERSION     DAJTUP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.314"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>773
                     /locus_tag="OO468_004231"
     CDS             <1..>773
                     /locus_tag="OO468_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6261096.1"
                     /translation="LSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAE
                     YYEVEEKKEKVYIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRVYKS
                     IITCTHILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIMSFN
                     ADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYMENP
                     EVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTV"
BASE COUNT          215 a          179 c          205 g          174 t
ORIGIN      
        1 ttgtcacgca atggctggaa agagttcgca gaggattacg gtattgatcc tgacaccttg
       61 ccatctttcc agaatccgaa cgatacatgg ctgtttccgt gggtgtcgaa tgatgtcgtc
      121 tacgtcgctg agtattacga ggtcgaagag aagaaggaga aagtatacat ctaccgcgac
      181 ccgctgacag gtgagccggt cagctattac caacaggata tcaaagacgt catcgacgac
      241 ctggctaatc gtggattcat taaggtagca gagcgcaagg tgaagcgtcg gcgtgtgtat
      301 aagtcgatca tcacctgcac gcatatactg aaagaccgcg agaagatagc tggagagcat
      361 atcccaatcg ttcctgtgta tggcgaatgg tcatttgctg gtgacaagga gtgctacgaa
      421 ggtgtagtaa ggctgacgaa agacggtcaa cgccttcgta acatgatcat gtcattcaac
      481 gccgatattg ttgctcgttc accgaagaag aaacctacct tcttccctga gcaaatcgaa
      541 ggctacgaat acatgtacgg tggaaatgat gactatccgt actatctcca gaacaggact
      601 gatgaaaacg gtaacgacct gccgattggt ccaatctcct acatggaaaa ccctgaagtg
      661 ccgcaagcca acgcttacat gcttgaggca gccaccaacg cagtgaaaga ggtggctagt
      721 cttggcgttg atgcgcaggc ggcaaatggt caggtcgctt tcgataccgt caa
//
LOCUS       DAJTUP010000307          771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTUP010000307 DAJTUP010000000
VERSION     DAJTUP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.315"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            54..>771
                     /locus_tag="OO468_004232"
     CDS             54..>771
                     /locus_tag="OO468_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR6261097.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQI"
BASE COUNT          213 a          170 c          179 g          209 t
ORIGIN      
        1 tcagtgaccc gataaaaatg acgctgtaag caagggataa ggataggcgt attatgatga
       61 cgaccagaat agtggttggc ctcacggcag ggacgtgtct gattttctcg caaaacctga
      121 tggccgaggt cagtgtattc aatccggcgc ttctggaaat caaccatcaa tccggagtcg
      181 atattcgcca gtttaatcgg gcaaacctga tgcccccagg tgtttatagc gttgatattt
      241 ttatcaacgg taaaatgttt gaacgtcagg atgtgacatt tgttcaggat aatccagatg
      301 ctgatctgca cgcttgcttt attgccatta aaaaaacact gtcctccttt ggcataaaag
      361 ttgatgcgct caaatcgttc aatgatgtgg atgagacggt ttgcctcgat cctgctccac
      421 gtattgaagg ctcatcctgg cagtttgaca gtgataaatt gcagctgaat atatccattc
      481 accaaatcta catggacgcg atggcttatg attacatcag ccccacgcgt tgggatgagg
      541 ggattaatgc gctcaccatc aactacgatt tttctggttc acatacacta cgttcagatt
      601 atggttcaca agagacagat accagttatc tcaatctgcg caatggactg aatattggac
      661 cgtggcggct acgtaattac agtactttaa acaccagcga tggccgtgcg gaatacaact
      721 ccattagtac ctggatacag cgcgatattg ccgcgttaag aagccagatt a
//
LOCUS       DAJTUP010000308          769 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTUP010000308 DAJTUP010000000
VERSION     DAJTUP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.316"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(161..769)
                     /gene="cusB"
                     /locus_tag="OO468_004233"
                     /pseudo
     CDS             complement(161..769)
                     /gene="cusB"
                     /locus_tag="OO468_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT          193 a          207 c          184 g          185 t
ORIGIN      
        1 ggtacacaat atcacatgga caacagcgca cagtaaacaa ctatatttga cgttcgtaga
       61 catactcagg tgaaaaaaag gttgtttttc gagttataac agacagttaa ttaactacag
      121 tggacaatgc tagacaatga cagacacaga taagcaacct accttcctct ttcgcgacca
      181 cgttatcttt ggcgatattc attcccgcgc gcagatcaaa cgcggtgatc acgccatcaa
      241 tgggcgcttt gagcgtaaag cgagtctgga ttttttgcgt ggcgatcagg tggcgaatat
      301 ccgcctccgg cattcccgcc agtcgcagcc gctcaagaat gccttcagtc tgggtcgccg
      361 taccgccggt ttcgcgcaac agtaaatact cactctgcgc ttccacccag tcgggaatgg
      421 tcaggtcgag aagcggtgtg cccttttgta ctttatcgcc cacggtaagc ggatacacct
      481 tgtcgataaa cccggcagcg cgagcctgca caatggcata ctgatactcg ttgtaactga
      541 cattcgtcgg gaaactctgg gcaaaagtca gcggcccgcg cgtgacggta gccgttttca
      601 cccccagatt ctgcgtctga gtcgggtcaa tgcgcacacc agacgcagaa ctctcttcat
      661 cggcatattt cggcaccaga tccatatcca taaacggcga tttacctggt ttatcgaacc
      721 gcgtattggg atacatcggg tcgtaccaga ataagatttt acgttctgc
//
LOCUS       DAJTUP010000309          756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTUP010000309 DAJTUP010000000
VERSION     DAJTUP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.317"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..229)
                     /gene="pgrR"
                     /locus_tag="OO468_004234"
     CDS             complement(<1..229)
                     /gene="pgrR"
                     /locus_tag="OO468_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR6261098.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILH"
     gene            367..>756
                     /locus_tag="OO468_004235"
     CDS             367..>756
                     /locus_tag="OO468_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6261099.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQD"
BASE COUNT          218 a          184 c          167 g          187 t
ORIGIN      
        1 catgcaatat agggccaaga acagacaaaa gatgctcgcc cgcttcagtg ggtacaacgc
       61 tgcgcgtggt tcgcgtcaga agccgcaatc ccaatcgttc ttctatacgg cgcactatct
      121 ggcttaaagc tgactgcacc atgctcaggc gggctgctgc gcgagtgaag ctacgctcct
      181 ctgcaacgac gacaaacgcc atcagatcag cgatttcttc acgcttcatt cagtatctcc
      241 atacaatcta ttaatcattt aaatgatagg tctaatcatg atttagggtc taatcaactt
      301 aatgatcaat atctatagtg atatcaccaa ccgtcaaaac gaaccgacaa ctccatgagg
      361 aactgaatga tgaataataa agtcagcttc actaacagca ataatccaac catctctttg
      421 tctgcagtga tctatttccc tccaaaattt gatgaaaccc gtcagtatcc ggcaattgtg
      481 gtatctcacc caggtggggg cgttaaagag caaacggccg gaacctatgc cgaaaagctg
      541 gcggaaaagg gatttgtcac tattgcttat gacgcatctt atcaaggtga aagtggcggc
      601 gagccgcgtc agttagaaaa cccatacatt cgtactgaag acgtcagtgc cgtgattgat
      661 tacctgacaa cgctttctta tgtcgacaat acccgaattg gtgcgatggg aatttgtgcc
      721 ggtgcaggat atacagccaa tgctgcaatt caggat
//
LOCUS       DAJTUP010000310          756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTUP010000310 DAJTUP010000000
VERSION     DAJTUP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.318"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..332
                     /locus_tag="OO468_004236"
     CDS             <1..332
                     /locus_tag="OO468_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6261100.1"
                     /translation="KHAEMAKAFDYILNHWNALNEFCRDGRVEIDNNIGENALRSVAV
                     GRKNYLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLP
                     WNFSSVK"
     gene            365..756
                     /locus_tag="OO468_004237"
                     /pseudo
     CDS             365..756
                     /locus_tag="OO468_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
BASE COUNT          197 a          194 c          199 g          166 t
ORIGIN      
        1 cgaaacatgc ggagatggcg aaggcgttcg actatatcct gaatcactgg aatgcgctga
       61 acgagttctg tcgtgacggc cgggtggaaa tagacaacaa catcggtgaa aacgcgttac
      121 gatcggtggc ggttggaaga aaaaattatc tctttttcgg ctcagacaag ggaggagaaa
      181 gtgcggcgat catctacagt ctgctggtca cctgcaaaca gaacgaagtg gagccggagg
      241 actggttgcg cgaagtgatc gagaagctca atgactggcc gtcgaaccaa gtgcatgaac
      301 tgctgccctg gaacttctcg tctgtaaaat aatccttacg ctacgtactt ctcggggcgc
      361 ttacaatgaa atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc
      421 cgtgatgaac tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat
      481 gcagactcgc ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag
      541 ctacagcggg ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc
      601 cgaattcgac caatgggtcg caaaagcgaa acagtcgccg aacaccatgt ctgacatggc
      661 tgcgttcgaa aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt
      721 gaaaccagac ttgtttgccg atgtgattaa caagtt
//
LOCUS       DAJTUP010000311          751 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTUP010000311 DAJTUP010000000
VERSION     DAJTUP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.319"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            36..>751
                     /gene="rlmB"
                     /locus_tag="OO468_004238"
     CDS             36..>751
                     /gene="rlmB"
                     /locus_tag="OO468_004238"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR6261101.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAV"
BASE COUNT          177 a          196 c          218 g          160 t
ORIGIN      
        1 gtgcatcagg caaaacgtaa acaacgagta cattaatgag cgaaatgatt tacggcatcc
       61 acgcagtgca ggccctgctg gagcgcgccc ctgaacgttt tcaggaagtc tttattttaa
      121 aaggccgtga agataaacgt ctgttaccgc tgattcacgc ccttgagtct cagggcgtgg
      181 ttatccagtt ggcaaaccgc caatatctcg acgagaaaag cgacggtgcc gtgcatcagg
      241 gcattatcgc ccgcgtgaag ccaggacgtc agtatcagga aaacgatctg ccggatctga
      301 tcgcttcgct cgatcaaccg ttcctgctga tcctcgacgg cgtaaccgat ccgcacaacc
      361 tcggcgcgtg cctgcgtagt gcggacgccg caggcgttca tgcggtgatt gtgccgaaag
      421 atcgctccgc acagctcaac gctacggcga aaaaagtagc ctgcggcgcg gcagaaagcg
      481 ttccgctgat tcgggtgact aaccttgcgc gcaccatgcg tatgttgcag gaagagaata
      541 tctggatcgt cggtacggca ggcgaggcgg atcatacact ctatcagagc aaaatgaccg
      601 gacgcctggc gctggtgatg ggcgcggaag gtgaaggtat gcgtcgcctg acccgtgaac
      661 attgcgatga gttgatcagc atcccaatgg caggaagtgt ttcttccctg aacgtttcgg
      721 ttgcaaccgg aatttgctta tttgaagcgg t
//
LOCUS       DAJTUP010000312          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTUP010000312 DAJTUP010000000
VERSION     DAJTUP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.320"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>747)
                     /locus_tag="OO468_004239"
     CDS             complement(<1..>747)
                     /locus_tag="OO468_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6261102.1"
                     /translation="IAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKW"
BASE COUNT          243 a          172 c          184 g          148 t
ORIGIN      
        1 ccatttcaaa gggaggatga tgagatcggg aataaaaaca aatacgacta ataatgaata
       61 taaaaccagg acgtaaggga aaacgcctct gactgcatca tcgaatttga gttttgccac
      121 ccctgaaata acgttaagta cattaccgat aggcggtgta attaagccga ttgagcagtt
      181 aatgataaac atgacaccga aataaatcgg atcgattcct gcctctttaa ctaaaggcat
      241 taataccggg gtaagaatta ataccgtcgg cgttaaatcc atgaccatgc cgacaatcag
      301 gatagccacc ataatgacga taaacagcag acgcggtgaa tcgaccaacg gttgcagtaa
      361 atcagaaacc atcatcggca gttcagcaat ggtaatcagc caggcggaaa cttgtgccga
      421 ggccaccaga aacataacca ctgaggtggt tttcgccgcg ccaatgagta catgccaaag
      481 tgtggcgaag gtcatttcac ggtaaataac tgtggcgaca aacagcgcat agaaagcagc
      541 aacggccccg gcttcggtag gggtaaacag accggagcga aacccgccaa taatgatgac
      601 tggaagaaac agcgcccaga taccggagac aaaagagtgc cagatttcct gcatcgttgc
      661 tttttgctgg cggggtaaat taaggcgact ggcctgccgc caccaggtaa gcatcagcgt
      721 tgcgcccatc attatgccag gggcaat
//
LOCUS       DAJTUP010000313          747 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTUP010000313 DAJTUP010000000
VERSION     DAJTUP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.321"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..296
                     /locus_tag="OO468_004240"
     CDS             <1..296
                     /locus_tag="OO468_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR6261103.1"
                     /translation="YWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDE
                     DPDDLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          213 a          158 c          176 g          200 t
ORIGIN      
        1 ggtactggaa agaaaatttc ccgcattcgt atgaagctat ttatggcaat tatcaaaata
       61 atgttttagc caatattatt ttcgtcgact tccagcaaca aggtgaaaga ggactgacga
      121 acgcgcctga tgaagacccg gacgatttaa gcacgggata ttacggttca gcgtaccggt
      181 caccggagaa ctggacgacg gcactgcgaa gcagtcattt cagcacggca gcccgtcggg
      241 ggattatttc tgacagattt gtagaagcaa ttttgcagtt ttggcgcgaa aggtgagcgg
      301 ggagtttatt catgggggat gtccttgttc gttctcagag tagacaacaa aaaacaaatg
      361 agatcaaggt aatatctctt cacttgcggg ctcgaacagc aggcaattgc caatataata
      421 atctggcaca agggtgaaaa acgcgcaccg cataaaccgt tgttattgct tacagattag
      481 ccggatgcct taatagacat ccgcgccttt tcgattatgg ctcggaaatc tacgaacatc
      541 tacacagttt actggaacgt tttgggccac agcgttcgca gcaccgtcct gatatgccgt
      601 tctggcgatt ataagtcgac ggattctggc aaatagacga tgatgatatg cttaatgatg
      661 ttcaaagaaa ttaggccatt attatattat tgcttgcatt ttctttcatt cctaaaagca
      721 cccgccatta atcccaggcg cattatg
//
LOCUS       DAJTUP010000314          743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTUP010000314 DAJTUP010000000
VERSION     DAJTUP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.322"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..743
                     /locus_tag="OO468_004241"
                     /pseudo
     CDS             1..743
                     /locus_tag="OO468_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
BASE COUNT          194 a          183 c          194 g          172 t
ORIGIN      
        1 ggcctcagcg tacgaatttc accgaagggg gtcatccagt tccagtaccg ctatcgctgg
       61 catggcaagc ctaatcgact tgggcttggt cgatacccat ccctgtcttt gaaggatgcc
      121 agacagatca ctgctgactt gcgaaagctc tatttctcag gaacggatcc acgcacctat
      181 tttgaagaga aggtggagaa ctccatgacg gtcgcccagt gtctcgacta ctggttcgac
      241 aactacgtct ctacaactct cagagaaaag acccaggcac tttatcgatc aacggttatg
      301 aagcgcatgc atgacgcctt ccctaatcgt ccggcatctt ctatcacggt taagcaatgg
      361 gttgacctgc ttaccgaaga agaaaaagat aatccacgcc gagcaaggca ggtgctaagt
      421 caactaagat cagcaattag ttggtgcatg cggcgtcagt tgatagatag ttgcgcaatt
      481 atgagcatcc agccaaggga cttcggctcc cgcgctgagg taggggatcg ggtactgtcg
      541 tatcacgaac tggctaagat ttggcttgct attgaaagaa gccgtgcgtc tacgtcaaat
      601 aagctccttc atcagatgct tatgctgtgg ggggcgaggc tctcagagct taggctggca
      661 acaaagacag aatttgacct gctggacaac gtatggacgt accgaaagag catagcaaga
      721 tgggtaatgt tatccgccgt cca
//
LOCUS       DAJTUP010000315          735 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTUP010000315 DAJTUP010000000
VERSION     DAJTUP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.323"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..107
                     /locus_tag="OO468_004242"
     CDS             <1..107
                     /locus_tag="OO468_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR6261104.1"
                     /translation="ACSAASAEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            462..710
                     /locus_tag="OO468_004243"
     CDS             462..710
                     /locus_tag="OO468_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR6261105.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          228 a          170 c          153 g          184 t
ORIGIN      
        1 acgcctgtag tgccgccagc gccgagcaac acaataacag cattaatcat atctacccgc
       61 gcatcgcccg tgtgcgtagc gtggaagaga tccttcacgc gttatgattt acatcggtct
      121 accgcaatgg tcacatccta aatgggtgcg gttggggatc accagccttg aagagtatgc
      181 ccgccacttt aactgcgtga tacgggattt ttaaaaatca ctaaagaacg cccaagagca
      241 tgtgttttct ttagaataat caaagcgttg gattgccatg tcatatgaaa atcggcaaag
      301 ttcatatgta acaaagtgtc ccaatcatgc cccaaaatgc cccacaacaa atatttttgc
      361 cccatgcatg ccccaaaaaa tcactttccc tcactcttga cactgtttat ccatacagtt
      421 aaaaataata ctgtataaaa acacagtgca gagggatttt tatgcgtttt gaaatctgca
      481 tagccaaaga gaaaatgact aaaatgccaa ccggtgctgt ggatgcgtta aaggaagaat
      541 taacccgacg catcagtaaa cgttatgacg atgtagaggt gatcgtaaaa gccaccagca
      601 acgatggcct ttctgttacg cgcaccgccg ataaagattc agctaaaact tttgttcagg
      661 aaactctgaa agatacctgg gagtctgctg acgagtggtt tgttcactaa ttagcacgta
      721 aaatcagtaa cggct
//
LOCUS       DAJTUP010000316          733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTUP010000316 DAJTUP010000000
VERSION     DAJTUP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.324"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..188
                     /locus_tag="OO468_004244"
     CDS             <1..188
                     /locus_tag="OO468_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1364 family protein"
                     /protein_id="HCR6261106.1"
                     /translation="AGTCGTATKPHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMH
                     AEGVFRTQEIWRKEGYL"
     gene            185..>733
                     /locus_tag="OO468_004245"
     CDS             185..>733
                     /locus_tag="OO468_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR6261107.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTV"
BASE COUNT          212 a          146 c          213 g          162 t
ORIGIN      
        1 tggcggggac gtgcggaaca gcgacaaaac cacacgatat gcaggcagcg attgcctgta
       61 gctcatgcca cgatttaatc gacgggcggg taaaaaccag cgattacacc aaagaagaat
      121 tacgcctgat gcatgcagaa ggtgtttttc gcacacaaga aatctggaga aaggaaggtt
      181 atttatgatt tacccaacaa atacaggcaa aagcggggaa caccttcgtc tcaccacgct
      241 ggaaagtgtc tggattcagg gaaaactgcg catgtggggg cgctggtcgt atattggcgg
      301 cggtaagacg ggaaatatgt ttaaccagtt gctggcatcc aaaaaattga cgaaaacagc
      361 cgtcaatgaa gccctgcgca ggatgaaaaa agcgggaata gagaaacctg agctggaagc
      421 gtttttgcga gagatgatca atggcaagca aaagacctgg ctggcgcatt gtactgatgc
      481 agaggcgtta tgtattgata gagtcataag tgaggtgctg gcagagcatc ctggattgat
      541 tagtgtcctc cggcaacggt atgaagggcg ggggatgact aagcgtaaaa tggctgaatt
      601 gctgaatgac gcacaccctg agtggtgttt ccggacgtgc tgcagtcggg tagatgtatg
      661 gctaaatctt gctgaatata tgctctatct accgatgcgt gatgcattct cttccgggga
      721 tctaaaaacc gtt
//
LOCUS       DAJTUP010000317          715 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTUP010000317 DAJTUP010000000
VERSION     DAJTUP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.325"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>715
                     /gene="ssuA"
                     /locus_tag="OO468_004246"
     CDS             <1..>715
                     /gene="ssuA"
                     /locus_tag="OO468_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR6261108.1"
                     /translation="IGMVLAKSHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLG
                     STGDIPPIFAQAAGADLVYVGVEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSS
                     SHNLLLRALRQAGLKFTDIQPTYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVR
                     VLKDGTDLNQTGSFYLAARPYAEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTM
                     GLPAPVIASYLDHRPPTTIK"
BASE COUNT          173 a          200 c          181 g          161 t
ORIGIN      
        1 tattggtatg gtgctggcaa aaagccacca gttactggaa aaacgctatc cgcaatcaaa
       61 aatctcctgg gtggagttcc ccgcgggtcc gcaaatgttg gaagcgttaa acgttggcag
      121 tattgatctc ggcagtaccg gggatattcc gccaatcttt gcccaggctg ccggggctga
      181 tttggtatat gtgggcgtcg agccaccgaa gcccaaagcc gaagtgattc tggtggcaga
      241 aaacagcccg atcaaaaccg tagccgatct taaaggtcac aaagttgcct ttcagaaagg
      301 ttccagttca cacaaccttt tactgcgtgc actgcgtcag gccggactta aattcaccga
      361 tatccagccc acttacctga cgcctgccga tgcccgcgcc gcgttccagc aaggtaacgt
      421 tgacgcctgg gctatctggg atccctacta ctccgctgcg ttattacagg gcggcgtgcg
      481 ggtgctgaaa gacggcaccg atctcaatca aactggatcg ttttatctgg cagctcgtcc
      541 ctatgcagaa aaaaacggcg cttttattca gggcgtactg gcaaccttta gtgaggccga
      601 tgcgttaacc cgcagccagc gcgaacaaag catcgcttta ctggcaaaaa cgatgggctt
      661 accggcaccg gtgattgcct cttacttaga tcatcgccct cctaccacca tcaaa
//
LOCUS       DAJTUP010000318          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTUP010000318 DAJTUP010000000
VERSION     DAJTUP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.326"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            178..471
                     /locus_tag="OO468_004247"
     CDS             178..471
                     /locus_tag="OO468_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR6261109.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(512..>697)
                     /locus_tag="OO468_004248"
     CDS             complement(512..>697)
                     /locus_tag="OO468_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6261110.1"
                     /translation="DQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTL
                     MLLTALLVTAKVKMKKS"
BASE COUNT          228 a          159 c          142 g          168 t
ORIGIN      
        1 tcgctgcggt ttaaaaaacg aaacgctatc gataagaata aaaaggaata aaagtggaat
       61 ataaagtctg gcacttctta ctgaccacgc aagcgcgttt cgtacagcat gatgagagcg
      121 atgaaagcaa actacactta tgcttcattc gatacacatt tgtaaaaggt tgaatccatg
      181 aaaccaacga cactactgct tattttcact tttttcgcta tgccaggcat cgtatacgcc
      241 gagtcgcctt ttagctcact gcagtccgca aaagagaaaa ccacggtatt acaagattta
      301 cgtaaaattt gcacgccaca tgcgtcatta tcagatgaag cgtgggaaaa gttaatgttg
      361 tcagatgaaa acaataaaca acatatacgc gaggccatcg tggcgatgga acgtaataat
      421 cagagcaatt attgggaagc gttgggtaag gtagaatgcc cggatatgta agaacagcaa
      481 caactactga ccaacgcttc tttacctgat atcaggattt cttcattttc acctttgcag
      541 taaccaacaa tgcagtcagc aacatcaatg tgccggacaa catcaacggc gaagtcagac
      601 caatattatc cagcgcataa ccgccgattg ccgcgccaca ggtattagca agctgaataa
      661 ccgccacctg aatagacccg gctttttctg cctgatc
//
LOCUS       DAJTUP010000319          696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTUP010000319 DAJTUP010000000
VERSION     DAJTUP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.327"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..673
                     /locus_tag="OO468_004249"
     rRNA            <1..673
                     /locus_tag="OO468_004249"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          175 a          164 c          206 g          151 t
ORIGIN      
        1 ttaagtcgac cgcctgggga gtacggccgc aaggttaaaa ctcaaatgaa ttgacggggg
       61 cccgcacaag cggtggagca tgtggtttaa ttcgatgcaa cgcgaagaac cttacctggt
      121 cttgacatcc acggaagttt tcagagatga gaatgtgcct tcgggaaccg tgagacaggt
      181 gctgcatggc tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc
      241 aacccttatc ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata
      301 aactggagga aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca
      361 cgtgctacaa tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa
      421 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
      481 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      541 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      601 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      661 gatcacctcc ttaccttaaa gaagcgttct ttgtag
//
LOCUS       DAJTUP010000320          695 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTUP010000320 DAJTUP010000000
VERSION     DAJTUP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.328"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>695
                     /locus_tag="OO468_004250"
     CDS             <1..>695
                     /locus_tag="OO468_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR6261111.1"
                     /translation="KPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINT
                     AFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQS
                     EIYARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNG
                     KTKTGRLWVYVRDDRNAGSSLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYN
                     VLYETGRVKEAGC"
BASE COUNT          178 a          175 c          197 g          145 t
ORIGIN      
        1 aaaaccgctg ccggcagaac ttccccggga gacacatcgc cttctgcctg ctgaaaccag
       61 ttgcccggcc tgtggaggtg ttctgaaaga aatgggggaa acaatctcag agcaactgga
      121 tatcattaat accgccttta aagttatcga aaccatacgt cccaaactgg cctgtagccg
      181 gtgtgatgtc atcgttcagg caccacttcc tcctaaaccg atcgaacgcg gttatgccag
      241 tgcagggtta cttgcacgga tcctggtcag caaatatatg gaacatatcc ctttatatcg
      301 ccagtcagaa atatacgcgc gacagggcgt ggagctgagc cgtaatacca tggtgcgctg
      361 ggtatcagaa atggcagaca aactccgtcc tctgtatata gcgctgaatg actatgttct
      421 ggaggcagga aaggtgcacg cagatgacac tccggtgaaa gtactggccc cggggaacgg
      481 aaagacgaaa acgggtcgtc tgtgggtata cgtcagggat gatcgtaatg cgggttcatc
      541 cctgccggca gccgtctggt tcgcgtattc ggcagatcgc aaaggagaac atccgcagct
      601 ccacctggca aagtatcagg gcgtactgca ggctgatgcc tatgcaggtt ataacgtact
      661 gtacgaaacg ggccgggtga aggaagccgg gtgcc
//
LOCUS       DAJTUP010000321          683 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTUP010000321 DAJTUP010000000
VERSION     DAJTUP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.329"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>683
                     /locus_tag="OO468_004251"
     CDS             <1..>683
                     /locus_tag="OO468_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR6261112.1"
                     /translation="NIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCEEI
                     NRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCTNF
                     VFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQELC
                     GVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERVMD
                     RMVMNGGRWV"
BASE COUNT          168 a          160 c          214 g          141 t
ORIGIN      
        1 aatattgcga caggaggcgt tctggagcgt atccgcagac tgaccccacc acatgtaacc
       61 gccccattca gaacggttgc ggagtggcgc gagtggcaac ttgctgaagg ccagaaacgt
      121 tgcgaggaga tcaaccgcct gaatcgtcag ttgcgggtgg aaaaaatcct gaaccgctcc
      181 ggcatccagc cgttgcaccg gaagtgttcg tttgcgaatt accgggcgca gaacgacggt
      241 cagcgacatg cactgagtca ggcgaaatcc atagctgacg aactgatgac aggctgtacg
      301 aattttgtgt tcagcggtaa gcccggcacc ggaaaaaatc atcttgcagc cgcgattggc
      361 aaccggctga tggcgaaggg gcgtagcgtg attatcgtca ccgtgtccga tgtcatgagc
      421 gtgttgcatg agagctacga caacggcaaa tccggtgaaa aatttttaca ggagctttgt
      481 ggtgttgacc tgctggtcct ggatgaaatt ggcatgcagc gggatacgaa aaacgagcag
      541 gtggtactga accagattgt tgatcgccgg acggcatcgt tacgcggtgt ggggatgctg
      601 acaaatatta accatgcagc gatgaataca cttctcggcg agcgggtgat ggatcgcatg
      661 gtcatgaacg gcgggcgctg ggt
//
LOCUS       DAJTUP010000322          675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTUP010000322 DAJTUP010000000
VERSION     DAJTUP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.330"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
BASE COUNT          207 a          153 c          149 g          166 t
ORIGIN      
        1 agggaaaccc ataaccaccc gatacgtggg aactaatcgc tttttaactg ttccgcgtgc
       61 gctcagccgc attcaccgca tcacaaaatt cactttaaaa agggcggaca tcagccagca
      121 atgaaactga tgccgccaaa ggtaccaatc aacatggagt gttgtggcgt ggttgtcact
      181 taagcgtatg gtcaacctga caacccggtg tcctcaacgg ggaaggaata actccgccat
      241 acttaccgcc gcgtcatttc gcgttgtgtg cctgctttta actacgtcag gcgaggtggt
      301 tcctgttatt ccccaacaac aagaaatttg tataatctga ataccccaac aataagaaga
      361 gtgagcaagt gatgtattag cgatgtcagc tctcaaagag actgcatcaa tgttaaaatt
      421 attcaatgaa gcaaaaactc aggctgaggt tgatcgcgct gtgtctgaaa taaactctaa
      481 gttgtcaagc attcagcttg aatatttgtc atctcattga tgagctgaca atctctaaaa
      541 gcaatgaggc tgcgctcaaa gcaaaactca cggagataga aaagtttaag ttccaaagtg
      601 aagactgcga acctcgctaa ttgattactg gaacgattgt gtatgccaga cagtgcaata
      661 ctagctctgg caaga
//
LOCUS       DAJTUP010000323          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTUP010000323 DAJTUP010000000
VERSION     DAJTUP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.331"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            88..606
                     /locus_tag="OO468_004252"
     CDS             88..606
                     /locus_tag="OO468_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR6261113.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
BASE COUNT          188 a          148 c          172 g          164 t
ORIGIN      
        1 ggatttatat gtttgaaaat tattatattg gacgagcata cagaaaaagc aaatcacctt
       61 tacatataat agcgtggaca aaaaacagtg aacattaata aagataaaat tgtacaactc
      121 gcagataccg acactattga aaacctgaca tccgcgttga gtcaaagact tatcgcggat
      181 caattacgct taactaccgc cgaatcatgc accggcggta agttggctag cgccctgtgt
      241 gcagctgaag atacacccaa attttacggt gcaggctttg ttactttcac cgatcaggca
      301 aagatgaaaa tcctcagcgt aagccagcag tctcttgaac gatattctgc ggtgagtgag
      361 aaagtggcag cagaaatggc aacaggtgcc atagagcgtg cggatgctga tgtcagtatt
      421 gccattaccg gctacggcgg accggagggc ggtgaagatg gtacgccagc gggtaccgtc
      481 tggttcgcgt ggcatattaa aggccagaac tacactgcgg ttatgcattt tgctggcgac
      541 tgcgaaacgg tattagcttt agcggtgagg tttgccctcg cccagctgct gcaattactg
      601 ctataaccag gctggcctgg cgatatctcc aggccagcca ttggtggtgt ttatatgttc
      661 aagccacgat gt
//
LOCUS       DAJTUP010000324          648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTUP010000324 DAJTUP010000000
VERSION     DAJTUP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.332"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            129..635
                     /gene="mug"
                     /locus_tag="OO468_004253"
                     /pseudo
     CDS             129..635
                     /gene="mug"
                     /locus_tag="OO468_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT          157 a          145 c          185 g          161 t
ORIGIN      
        1 ggccatggta gcggattata aagtaactcc gtgccgcaat ccagccatta ccgcgcgcct
       61 gctgttttta taaacaatgt attttcaatc cgttatactt ttcctggtga tgttagaaag
      121 gagtaaatat ggttgaggat attttggctc cagggttacg ggtcgtgttt tgcggtatca
      181 accctgggct ttcatccgcc gggactggtt ttccctttgc tcatccggca aatcgcttct
      241 ggaaggtgat atatcaggcc gggtttaccg accgtcagtt gaagccgcag gaggcacagc
      301 atctgctgga ttatcgttgt ggcatcacca aactggtaga ccgtccaacg gtgcaagcca
      361 atgaagtttc aaagcaggag ctgcacgcag gtgggcgtaa gctgattgaa aaaattgaag
      421 attatcagcc gcaggcgttg gctattctgg gcaaacaagc atatgaacag ggattcagct
      481 agcgcggtgc acagtggggg aaacaaacgc tcaccattgg ttcgacgcag atttgggtgc
      541 tgccaaatcc cagcggttta agtcgcgttt cactggagaa actggttgaa gcgtatcgcg
      601 agctggacca ggcgctggta gtgcgtgggc gataaaaaac gtcaccgg
//
LOCUS       DAJTUP010000325          635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTUP010000325 DAJTUP010000000
VERSION     DAJTUP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.333"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            18..491
                     /locus_tag="OO468_004254"
     CDS             18..491
                     /locus_tag="OO468_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR6261114.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          127 g          185 t
ORIGIN      
        1 gagatgaacc ctatgcaatg tcctggttaa tatctcagtg tgctcatcag tgcactgata
       61 ataaaaagac ggagacagat gccatttacg ataaagttcg gagttcttat ttgctttcat
      121 gtattctgaa aaaaaacaaa aatgttgggc ttattcttca tgcaccatca tttgtttctg
      181 tgtctgaaaa aatcgccaga attgtgatgg caaattactc ccgtaactgg agtaattccg
      241 agttggccag tgcggtttta atgagtgaat cttctttaaa aagaagaatg tacaaagagg
      301 tcggttctat aagtacattt gtacataaaa taaagcttac agaagctatc aggaaattac
      361 gaaggacaaa tactcccatt tctgtcatat catctgagtt aggttacagt tcaccatcgt
      421 atttttctaa agtcttcttt aaatatttaa agacataccc tcaaaacata aggaagaaaa
      481 atggtcggta atgtcaccaa ttaggaggac ttgcagagcc gcttttttac gatactgaga
      541 tcaaacagac ataatctatg cggagattta cagcagggca tggttcacaa atctgacagc
      601 gatgaattat cggcactgcg ggcagaaaat gctcg
//
LOCUS       DAJTUP010000326          634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTUP010000326 DAJTUP010000000
VERSION     DAJTUP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.334"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..356)
                     /locus_tag="OO468_004255"
     CDS             complement(<1..356)
                     /locus_tag="OO468_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6261115.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRL"
     gene            complement(358..>634)
                     /locus_tag="OO468_004256"
     CDS             complement(358..>634)
                     /locus_tag="OO468_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261116.1"
                     /translation="DYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAGIY
                     KTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
BASE COUNT          172 a          171 c          150 g          141 t
ORIGIN      
        1 atcagccgcc ggtatacagc atcagacagg tcaacagtcc gggtatccga ttttccgttg
       61 taaaacggtg cctgattaaa cggacaggga tcatccatta ccggggtgtc catcctggac
      121 tcgctgaagc ccaggtaatt aaaatcgtcc tttaccgtca gccagcgact gacacccaca
      181 atctttcccc agacatcaag accatacttt tctgcggtat cgatgttcca gataagacca
      241 taaaaatcac tgataaaact gtcgggggaa agcgctgcgt taaagctgtt aatgagggca
      301 ttgagtcggg ggctggcagc atactgcgca agcacagtgg cagccacatt ctgcacgtta
      361 tgcctcctgg agtttcacac tgatatccga cacatccaga accggaatct catctatccc
      421 gaaagtgaca gccgttgtcc atgacgaacc atcacggctc acagtaaggc ccagaatatc
      481 aatatgttcc ggatcggttt tgtaaatgcc ggcatagtag cgcccggcag agacaacaga
      541 ggcaactctt gcccgcagac caccatctgt accattaaat gcgtccagca cggattgctg
      601 tacctgtcgg gtaatatctg aaggcaggta gtca
//
LOCUS       DAJTUP010000327          619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTUP010000327 DAJTUP010000000
VERSION     DAJTUP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.335"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>619)
                     /locus_tag="OO468_004257"
     CDS             complement(<1..>619)
                     /locus_tag="OO468_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6261117.1"
                     /translation="FVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALP
                     QELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLA
                     DEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLG
                     GEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIA"
BASE COUNT          150 a          178 c          171 g          120 t
ORIGIN      
        1 gcaataccct gcagtttcgg gtgatcgctg agatatttca gggaggcttt gtagtcttcc
       61 agcaacagtt cagcaaagtc catttcgaag ccgcgacgac acataatgcg catcaccacg
      121 atgtcggtgg cttcaccgcc gagagtgaag gccggaacct gccagccgcg cagacgcaga
      181 cgttcagaga ggtcgtacag ggtgtatccc ggatcttcac catctttcag tttgaagcaa
      241 accgccggga tgccttcgtc cgggcgaccg gtacagatga actcatacgg ccccagtttg
      301 gcgatctcat ccgccagata ggcagcgacc tggtaggaag cattctgtac tttggtatag
      361 ccttcacgac caaggcgcag gaattcatag tactgtgcaa tcacctgacc cgccgggcgg
      421 gagaagttga tggcaaaagt accaatctga ccaccgaggt agtcaacgtt gaacaccagt
      481 tcctgcggca gcgcttcttc gtcacgccag ataacccagc cgcagcccag cggagccaga
      541 ccgaatttat ggcctgaagc actgatcgat ttcacacgcg gcaggcggaa gtcccagacg
      601 atatccgggg cgacgaacg
//
LOCUS       DAJTUP010000328          619 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTUP010000328 DAJTUP010000000
VERSION     DAJTUP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.336"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..450
                     /locus_tag="OO468_004258"
     CDS             <1..450
                     /locus_tag="OO468_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR6261118.1"
                     /translation="AERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPIDLADARE
                     LVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIERQLLMVL
                     LENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
BASE COUNT          161 a          127 c          180 g          151 t
ORIGIN      
        1 gcggagcggg cgctgaacag tgttgaggcg tcacaaccgc atcaggatca aatggagaaa
       61 tcagcaaaag cgttaaccca acgttgggat gactggtttg ccgatccgat tgaccttgcc
      121 gacgcccgtg aactggtaac agatacacga caatttctgg cagatgtacc cgcgcatacc
      181 agctttacta acgcgcaact gctggaaatc atgatggcgc aggattttca ggatctcacc
      241 gggcaggtta ttaagcggat gatggatgtc attcaggaga tcgaacgcca gttgctgatg
      301 gtgctgttgg aaaacatccc ggaacaggag tcgcgtccaa aacgtgaaaa ccagagtttg
      361 cttaatggac ctcaggtcga taccagcaaa gccggcgtgg tagccagtca ggatcaggtg
      421 gacgatttgt tggatagtct tggattttga tttgtattgc ctgaacttcg gcctgcgcga
      481 tgatgccaag ccgatagcag ggtggcgggt atcgttcagg gagtacgtgg aggatggtta
      541 tttgtagttt gataatttta gatcagcgaa gcgtgattgt tgcgccagta aatttatcaa
      601 tgcattaaat tgtgaacga
//
LOCUS       DAJTUP010000329          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTUP010000329 DAJTUP010000000
VERSION     DAJTUP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.337"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..400
                     /locus_tag="OO468_004259"
     CDS             <1..400
                     /locus_tag="OO468_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6261119.1"
                     /translation="DISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            400..>617
                     /locus_tag="OO468_004260"
     CDS             400..>617
                     /locus_tag="OO468_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR6261120.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQG"
BASE COUNT          170 a          143 c          155 g          149 t
ORIGIN      
        1 cgatattagc agcgaagaat acgccattat gccactggct ccaatgaagg aggccgatgc
       61 gccgaacgaa gaactgcgtc aggcctggga gtattaccac acacctcgtg cgcagtatcc
      121 aacagcaccg ggttacgcta ctttgcgcag ccttaaccag attattacct atgatgctta
      181 ccatatggcg gaagtgtacc tgactcagcc gatgcagatt gtggcgggca gccaggcagg
      241 gagcaaatgg atgagtgacg acctgtacga tcgggcatca agccaggata aacgctatca
      301 cattgttgaa ggcgcaaacc atatggatct ttatgacggc aaagcctatg tggctgaagc
      361 aatttcagta ttagctccgt tctttgagga aactctgtaa tgaaacatat tcaaatccgc
      421 aatagtgata tggcctggca tattgctgcc aatattcagt ttccaccaaa ttttgatgag
      481 tcaaaacagt atccggcgat tatcagcgtc catccttttg gcagttgtaa ggagcagacg
      541 tctggcaata tctatggcaa ggcattggct gaaaaaggct atgtcgttct cgcttatgac
      601 gccagtttcc agggaga
//
LOCUS       DAJTUP010000330          610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTUP010000330 DAJTUP010000000
VERSION     DAJTUP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.338"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(48..608)
                     /locus_tag="OO468_004261"
     CDS             complement(48..608)
                     /locus_tag="OO468_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6261121.1"
                     /translation="MIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSDTADQ
                     IVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDLKVGT
                     PLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEMKFIA
                     RYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          164 a          156 c          133 g          157 t
ORIGIN      
        1 aacgactccc tggccaaagc caactgaata aaaaggccct tgataaatta tttcttatat
       61 tcgacagtaa attgtgaatc agcgttcgcc gtaccagccg ttacggtcgc attggttgcc
      121 acatagcgtg caataaactt catttcagcc ttgccttcag catcgatagc ggtataagca
      181 gagtcgttat aaagtttaac ctgcgaatta tcggactggt taaaaatacg aataccaacg
      241 ccggttgccg caatcgcttg tcctgtaccg gtaaaatcac cagggttgcc tgctgtaagc
      301 ggcgtaccca ctttcaggtc gccgtcgccg ccaggcgctt ctgtaccatc aaaacgaacg
      361 gctgcttgag tgtacgttgc cgggcaattt tcaagtttaa tactgaactt ctgtggcgac
      421 gccagactac ctacacctga aaatgcattt ttactgactt tacccagtgg gacaatttga
      481 tcggctgtat cggaggtaac ttcacaagta gtgtcgacaa tttcaccgac gaagttaaca
      541 acaccatctt ctgccagtgc atgcccggcc agcattagag atgccagagt gaatgcaccg
      601 cctataatat
//
LOCUS       DAJTUP010000331          588 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTUP010000331 DAJTUP010000000
VERSION     DAJTUP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.339"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..588
                     /locus_tag="OO468_004262"
                     /pseudo
     CDS             1..588
                     /locus_tag="OO468_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          145 a          167 c          163 g          113 t
ORIGIN      
        1 cgtaaacagc attcgcctga atttcgcagt gaagccctga agcttgctga acgcatcggt
       61 gttactgccg cagcccgtga actcagcctg tatgaatcac agctctacaa ctggcgcagt
      121 aaacagcaaa atcagcagac gtcttctgaa cgtgaactgg agatgtctac cgagattgca
      181 cgtctcaaac gccagctggc agaacgggat gaagagctgg ctatcctcca aaaggccgcg
      241 acatacttcg cgaagcgcct gaaatgaagt atgtctttat tgaaaaacat caggctgagt
      301 tcagcatcaa agcaatgtgc cgcgtgctcc gggtggcccg cagcggctgg tatacgtggt
      361 gtcagcggcg gacaaggata agcacgcgtc agcagttccg ccaacactgc gacagcgttg
      421 tcctcgcggc ttttacccgg tcaaaacagc gttacggtgc cccacgcctg acggatgaac
      481 tgcgtgctca gggttacccc tttaacgtaa aaaccgtggc ggcaagcctg cgccgtcagg
      541 gactgagggc aaaggcctcc cggaagttca gcccggtcag ctaccgcg
//
LOCUS       DAJTUP010000332          587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTUP010000332 DAJTUP010000000
VERSION     DAJTUP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.340"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>587
                     /locus_tag="OO468_004263"
     CDS             <1..>587
                     /locus_tag="OO468_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6261122.1"
                     /translation="LSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVYVAE
                     YYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRVYKS
                     IITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIMSFN
                     ADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYL"
BASE COUNT          158 a          132 c          163 g          134 t
ORIGIN      
        1 ttgtcgcgca atggctggaa agagttcgca gaggattacg gtattgaccc ggataccctg
       61 ccatctttcc agaatcctaa cgatacatgg ctattcccgt gggtgtcgaa tgatgtcgtc
      121 tacgtcgctg agtattacga ggtagaagag gagaaggaga aggtcttcat ctaccgcgac
      181 ccgctgacag gtgagccggt cagctattac cagcaggata tcaaagacgt catcgacgac
      241 ctggctaatc gtggattcat taaggtagca gagcgcaagg tgaagcgtcg gcgtgtgtat
      301 aagtcgatca tcacctgtac gcagatactg aaagaccgcg agaagatagc cggagagcat
      361 attccaatcg ttccagtgta tggcgaatgg tcattcgctg gtgacaagga gtgctacgag
      421 ggtgtggtaa ggctgacgaa agacggtcaa cgccttcgta acatgattat gtcgttcaac
      481 gccgatattg ttgctcggtc accgaagaag aaaccgacct tcttccctga gcaaatcgaa
      541 ggctacgaat acatgtacgg cggaaatgat gactatccgt actatct
//
LOCUS       DAJTUP010000333          579 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTUP010000333 DAJTUP010000000
VERSION     DAJTUP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.341"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..105)
                     /locus_tag="OO468_004264"
     CDS             complement(<1..105)
                     /locus_tag="OO468_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR6261123.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAE"
     gene            complement(152..>579)
                     /locus_tag="OO468_004265"
     CDS             complement(152..>579)
                     /locus_tag="OO468_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HCR6261124.1"
                     /translation="LLKPIDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGH
                     SRIYLLQMKDVAFVSSRMSGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLA
                     HLQEIRLEDNGQAELILRNGLTVPVSRRYLKSLKEAIGL"
BASE COUNT          141 a          155 c          129 g          154 t
ORIGIN      
        1 ttccgcggtg gcttcgacat tatccagcgc cagaatacgc accaggtcat tattattggc
       61 tttcaaaatg tagcgcacgc tgcgcagaat atcgttactt atcatgaggc cttcaggtgt
      121 tgatgaggca aaaagccatt ttagcagtct tttacaggcc aatcgcctct tttaagcttt
      181 tcagataacg gcggctgacc ggcacggtta agccattacg caaaatcaac tcggcctggc
      241 cgttatcttc cagacgaatc tcctgtaaat gcgcgaggtt aaccagatac tgacgatggc
      301 agcgcagtag tggtgtacga ctttccaagg tacgtaatgt caattcggta aagccctctt
      361 tcccttcgtg gctggtaacg tagacaccgc tcatccgact gctgacaaat gccacatctt
      421 tcatttgcag caaataaatc cgactatgcc ccgtacaagg gataaatttc agcgcctgtt
      481 gattttccgg taacagcgaa acatcctgct tgctgcgctc ctgacgcaat cgcgccagcg
      541 ttttctccag tcgcgcttca tcaattggct tcagcagat
//
LOCUS       DAJTUP010000334          577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTUP010000334 DAJTUP010000000
VERSION     DAJTUP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.342"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..98
                     /locus_tag="OO468_004266"
     CDS             <1..98
                     /locus_tag="OO468_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6261125.1"
                     /translation="RLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            156..512
                     /locus_tag="OO468_004267"
     CDS             156..512
                     /locus_tag="OO468_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261126.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          183 a          110 c          149 g          135 t
ORIGIN      
        1 ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag cgtgtactgg
       61 ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct atcaggcttc
      121 aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca gaagatgagt
      181 taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag gttaattttc
      241 tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt gacgaaaaag
      301 agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac tggatgctct
      361 ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc caggaggaac
      421 aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg aaagcgcgga
      481 tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta aaaatttaat
      541 ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAJTUP010000335          566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTUP010000335 DAJTUP010000000
VERSION     DAJTUP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.343"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>566
                     /locus_tag="OO468_004268"
     CDS             <1..>566
                     /locus_tag="OO468_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR6261127.1"
                     /translation="FPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTVFQ
                     TYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTPIG
                     IQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQAD
                     AIRTKAQAEADAIRLRGEALRQNPNVME"
BASE COUNT          167 a          159 c          140 g          100 t
ORIGIN      
        1 tcttcccgac cttcaaacaa atgaagacct acgatgagcc attcagcttc cagatgagtg
       61 acggtacaac catcggctat cacatcggcg tggcctacaa agttgatcca tccaaagtta
      121 ccacagtctt tcagacctac cgcaaaggcg tggacgacat taccgacacc gacctgcgcc
      181 agaagatagc cgacgcactc aaccgactgg ccagcaaaat gaccaccgac aaatttatcg
      241 acggcggcaa gtctgagctg ctggatgcag ctcttaaaga cattcaggaa gaaatgacgc
      301 ccatcggtat tcaggtaatg agcctctcat atgtgggtaa gccggagtac ccgcctactg
      361 ttatcgacag cattaatgcc aaagtcacgg cgaaccagaa aaccctgcaa tgcgagcagg
      421 aagtaaaaca gcgcgaagcg gaagccaaca tgttgcgcgc ggaagctgcc ggacaggcag
      481 atgcgattcg cacaaaagcc caggccgaag ccgatgctat tcgtttacgc ggtgaagctc
      541 tgcgccagaa cccaaacgtt atggag
//
LOCUS       DAJTUP010000336          556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTUP010000336 DAJTUP010000000
VERSION     DAJTUP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.344"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..556
                     /gene="espL1"
                     /locus_tag="OO468_004269"
                     /pseudo
     CDS             1..556
                     /gene="espL1"
                     /locus_tag="OO468_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
BASE COUNT          191 a          107 c           96 g          162 t
ORIGIN      
        1 aaatatttat gcaagaaatt cagtcactgg tagataatca catcattcat gaagataatc
       61 tggttaaatt actgcaaact aaaagtgcta acgaaacacc tggactttat atctccatgt
      121 tgtatggatt cgatgaaata atcgatatct ttctgaatac attagccact ccaatagcac
      181 taagagcttt taaacaaaaa actggtgatg agtattttag ccatgaaaat acatgatggt
      241 gagccaggat tatacgccgc aatggaaaat aatcaccctt tgtgtgtcac acggttcctc
      301 tctaaaatta atggcatcgc ctttaaatac aagttgagca aagctaacat catggattta
      361 ttaaaaggag ctacagcaca gggaacccct gcattataca tcgccatgag caagggtaat
      421 gaagacgtcg tgttatctta tatatcgacg ctgggtgctt ttgcaaaaaa acattctttt
      481 agtcaacatc agttatttac actattggcc gctaaaaatc atgacaacat gtcagctgtt
      541 catatagcca ttcatc
//
LOCUS       DAJTUP010000337          551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTUP010000337 DAJTUP010000000
VERSION     DAJTUP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.345"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>551
                     /locus_tag="OO468_004270"
     CDS             <1..>551
                     /locus_tag="OO468_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR6261128.1"
                     /translation="DTENIYLLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDT
                     CREFNGITTTGRMLYRNLKITSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPV
                     EDYTVHEITLGPGCNVPGYAGTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGV
                     ANSSGFALAALLNANIEMGNDPI"
BASE COUNT          178 a          106 c          112 g          155 t
ORIGIN      
        1 tggatacgga aaatatctat cttctgcctc ttgctcgtgg caataaccat aattacgatg
       61 gaaaaagcgt tgtcgaaatt agaaaactta atattggcaa acagtcctgg ccatttaatt
      121 atgtgacaga tacatgcaga gaattcaatg gtattacaac aacaggacgt atgctctatc
      181 gaaacttgaa aatcacctca gctttagacg aaatatatgg tggtatatgt aaaaaggcgc
      241 atgctgctac agagttagca gaaggtttac ggctaaattt atttatgaag agccctttcg
      301 atcctgttga ggactataca gttcatgaaa taacattagg ccctggttgc aatgttcctg
      361 gttatgccgg aaccacgata ggatatattt caacccttcc agcttctcag gcaaaaagat
      421 ggaccaatga gcagccaaga attgatatct atattgatca gataataact gtctcagggg
      481 tagcaaattc ttccggattc gcccttgccg cattacttaa tgctaatatt gagatgggaa
      541 atgacccaat c
//
LOCUS       DAJTUP010000338          545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTUP010000338 DAJTUP010000000
VERSION     DAJTUP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.346"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(54..>545)
                     /gene="fdhD"
                     /locus_tag="OO468_004271"
     CDS             complement(54..>545)
                     /gene="fdhD"
                     /locus_tag="OO468_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR6261129.1"
                     /translation="LAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLN
                     DFQPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLV
                     SSRASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQR
                     LSN"
BASE COUNT          135 a          166 c          122 g          122 t
ORIGIN      
        1 tcacgaaatg aataagtcct gcaatttaat atatatcgca ggacttattt taattaattg
       61 ctcaaacgct gcggatgggt ataaaccgtt gcccgacccg gtttacaaaa acctaccagt
      121 gtcagattac agcgctcagc cacttctaca gcaagcgtgg tcgcggcaga caccgcaaac
      181 aaaatctcta cgccgcacat cgccgacttt tgcaccattt cataactggc acgactggaa
      241 accagtaccg caccttgctg ccagctttcc ccttcttgtg accggcggcc taacagtttg
      301 tccagcgcca catggcgacc cacgtcttca tgcccgccga ccagttcgcc agatggcaac
      361 atccaggcag cggcgtgagt acaaccagtc agtcgcccca ctggctgaaa atcgttgaga
      421 tgacgtaatg catcatccag tttgttgaga tcaaacgtct gggtgaacgg tagcggctgc
      481 accggttttc cgatgtcatt aagttgctcc acgccgcata cgccacagcc cgtacgtccc
      541 gccag
//
LOCUS       DAJTUP010000339          544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTUP010000339 DAJTUP010000000
VERSION     DAJTUP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.347"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(194..532)
                     /locus_tag="OO468_004272"
     CDS             complement(194..532)
                     /locus_tag="OO468_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR6261130.1"
                     /translation="MQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQ
                     LQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVL
                     IEAMESRSLR"
BASE COUNT          153 a          120 c          137 g          134 t
ORIGIN      
        1 cgactcttgt gatttatatg tttgttttcg gtgaatatat tgttaattaa catcaatcat
       61 cagagagacg gcatcgggaa aggacaagtc tgtggagggc ttcataaaaa caagtgtaaa
      121 aggaataaaa ggcagaaata aaagtcagga gtcatgcccg tttgcaaaca agtggttcac
      181 aaacgggcaa tttttaacgt aacgagcgac tttccatcgc ctcgatgagc accgcatcat
      241 gtttcagcgt ttgccatgca tggatgactt ctgccggaaa atcaacgtga acgataaaat
      301 ctcgcccgcg gggaccgtag ccatcgctat catcgtgtaa acgcggcagc tcgccagtga
      361 gtagtgagag ataacgctca accggccata atccttgtaa ctgattaata aagattacac
      421 tgtctgcgtt atttcctaac tgaggaacga atccgggata acttaaccag cgtggtgcat
      481 cgggattgtc cccggtcata cggcggaacg ttgccatcgt gcgccgctgc atggtggggt
      541 cctg
//
LOCUS       DAJTUP010000340          542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTUP010000340 DAJTUP010000000
VERSION     DAJTUP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.348"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..542
                     /locus_tag="OO468_004273"
                     /pseudo
     CDS             1..542
                     /locus_tag="OO468_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
BASE COUNT          134 a          146 c          120 g          142 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 ct
//
LOCUS       DAJTUP010000341          541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTUP010000341 DAJTUP010000000
VERSION     DAJTUP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.349"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>541
                     /locus_tag="OO468_004274"
     CDS             <1..>541
                     /locus_tag="OO468_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6261131.1"
                     /translation="IYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTTHGIASVN
                     GDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTGTQEKITK
                     DKDIVFTNGGNVLFKDNLDFGCGGIIFDEGHEYNINGQRFTFKGAGIDIGKESIVNWN
                     ALYSSDDVLHKIGPGTLNVQ"
BASE COUNT          212 a           77 c          106 g          146 t
ORIGIN      
        1 gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca gcggctctgg
       61 agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa cccatggtat
      121 tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata aactggttag
      181 tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg taaccattaa
      241 aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc aagaaaaaat
      301 aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta aggataattt
      361 ggattttggt tgcggtggta ttatctttga cgaaggccat gaatataaca taaacggtca
      421 gagatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg taaactggaa
      481 tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc tgaatgttca
      541 a
//
LOCUS       DAJTUP010000342          534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTUP010000342 DAJTUP010000000
VERSION     DAJTUP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.350"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO468_004275"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO468_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR6261132.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
BASE COUNT          147 a          116 c          102 g          169 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atga
//
LOCUS       DAJTUP010000343          528 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTUP010000343 DAJTUP010000000
VERSION     DAJTUP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.351"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            26..409
                     /locus_tag="OO468_004276"
     CDS             26..409
                     /locus_tag="OO468_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261133.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            406..>528
                     /locus_tag="OO468_004277"
     CDS             406..>528
                     /locus_tag="OO468_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate transporter"
                     /protein_id="HCR6261134.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLIL"
BASE COUNT          128 a          144 c          147 g          109 t
ORIGIN      
        1 gtctggcggc tgaggaacat atactatgta cgcaaaatct tttctcgcac ttgatggcaa
       61 cggacgtctg accggtgccc gtaccgcaca gactgcacct tacgatagct acacctgcca
      121 cctttgcggc agtgcactca gataccaccc gcaatacgac actgaacggc cctggtttga
      181 acacgctgac gaagggctga cggagcacgg tcatgagtgc ccttatgtca ggccggaacg
      241 cagagaaata cggttgatta aacgtctgca gcaattcgtc ccggatacct tacccgtagt
      301 acgtaaagcc agctggcact gcaggcaatg tcaccacgat tattatgggg agcagtactg
      361 cacacactgc cagaccgggc gcttcagtga ggagggggga gcagaatgaa cggtataaaa
      421 acggggctgg ggatcacgcc gggtgagcac attatcagtg ccgactctgc gctcagcaga
      481 aatatccggc agtgtttctg cctttcctgc catggccggt tgattctg
//
LOCUS       DAJTUP010000344          522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTUP010000344 DAJTUP010000000
VERSION     DAJTUP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.352"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..336)
                     /locus_tag="OO468_004278"
     CDS             complement(<1..336)
                     /locus_tag="OO468_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR6261135.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVY"
BASE COUNT          170 a          103 c          119 g          130 t
ORIGIN      
        1 ataaaccgcg cctgcttctg aaggtttaat gtggaagctg actgatactg tcatttgtgc
       61 cggttgctgg tcgcggctgt aggcctgtaa tccctgatag accacagcct gattacgcgt
      121 ggaaattttc tccacgcttt ccataaaagg gatcttaaaa cccagccccg gttcggcaac
      181 ttttacgatt tttccgtagc gcagcaaaat gccgcgttca ccttcattaa cggtgtaata
      241 cgagagaaat ggcagcacaa ccacggcaag cacgccaatg gcgatagcaa gagatttttg
      301 tgggcgaaat gaggttaatg aaacaggtgc tttcatgaca atcttccttg agtaaagtat
      361 gggaatatgt cacatgagat gaggagaaaa cagccgacag gtgagaattc agaatctata
      421 aaggggtata gcgaaaaaac attgcattag aaaagtatgt tttcgaacat tcaataaata
      481 aatagagata tgcatcacaa atacaataac aaacaaaatg tt
//
LOCUS       DAJTUP010000345          519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTUP010000345 DAJTUP010000000
VERSION     DAJTUP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.353"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..519
                     /locus_tag="OO468_004279"
                     /pseudo
     CDS             1..519
                     /locus_tag="OO468_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          129 a          133 c          142 g          115 t
ORIGIN      
        1 aacccgccac accacgggcc ggacgcccat gggctgagca ccgtaaaatc atcaatggca
       61 tgttctgggt gttatgttcc agtgcaccat ggcgcgattt acccgaacga tatggggcat
      121 ggaaaactgt ttataaccgc tttaaccgat ggtcaaagtc aggcgtgatt aatattattt
      181 tcaacaggtt gctttcgcta cttgatgcaa acggctttat tgactggtct gccaccgcgc
      241 tggatggcag taatatccgg gcgctgaaat gtgccgccgg tgctcaaaaa aacatcccga
      301 tatcgacgga gataatgggc cgggtcgctc tcgcggcggt tttggcacca aaatccatct
      361 ggcaacagac ggaagtggcc tcccgttaaa tatcgtgcta agccccggac aagctcatga
      421 aagccagttc gcgcaacgac ttctggacgg gattggcgtt cagcgtcaga acggcagcat
      481 gaaacgccgt ggacatgcgg tactggctga taaagcgta
//
LOCUS       DAJTUP010000346          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTUP010000346 DAJTUP010000000
VERSION     DAJTUP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.354"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(35..307)
                     /locus_tag="OO468_004280"
     CDS             complement(35..307)
                     /locus_tag="OO468_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261136.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          117 a          129 c          145 g          127 t
ORIGIN      
        1 tgtgaacagg cgtttcagcg gtcggggctg aaaatcacca ccacttcggg tcatccgcct
       61 tcatctccgc ttctgtttcg tataaatcaa aacgacggca ggtatggcag aacgtgacgt
      121 attcgtacgg tgaatccggg tacattttgt ggaaggtttc ccagaaacag accgtgcagg
      181 agggatgacc ggtgataaag tctgcaaata cggacttata agggtgatta ttggctctgg
      241 cgacggcttt cagaacctct ttcaccattc tggtgtggac tttctggtgc tccaggttgt
      301 gtgacatggg aactcattct ggatggttac tctgaaagct catattctgc cacaccccga
      361 tttgcagccg ccaggctgcc gtggttcaca gataaaacac tctccaggaa acccggggcg
      421 gttcactgcg tgtctgggtt cgccagcatg agcgggatac cgggggcggt tcacacgaac
      481 ataccacaga cagttgattt tggtacgccc aggcgacg
//
LOCUS       DAJTUP010000347          516 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTUP010000347 DAJTUP010000000
VERSION     DAJTUP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.355"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..164
                     /locus_tag="OO468_004281"
     CDS             <1..164
                     /locus_tag="OO468_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR6261137.1"
                     /translation="AYVLNNGGLRMSYVFESALSLAELAIIQSSGALPSPPGVYVSVV
                     LKESRNEGQ"
     gene            151..>516
                     /locus_tag="OO468_004282"
     CDS             151..>516
                     /locus_tag="OO468_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6261138.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDP"
BASE COUNT          117 a          129 c          152 g          118 t
ORIGIN      
        1 gggcttatgt tctgaataat ggcgggctga ggatgagtta cgtctttgag tccgcgctct
       61 cgttggcaga actggcgatt atccagtcgt cgggtgcact gccatccccg ccgggtgttt
      121 atgtttcagt agttttaaag gagtcccgta atgaaggcca gtgataaacc ccgccagctg
      181 gcggtcccct ttgcgagtac cggagataaa aaccgtatcc cggacaaggc gacacagcag
      241 accagagaga gcggtaatgc tgcgtatgat tcaggttttc ctccggtgac catgacagcg
      301 gtctcagcgg gaggtatacc gccacacggc aaggatttta acggtctgat gtacgatatt
      361 accgcagcaa tacggttcgc ccaggctggc ggtttgtaca cgtataatgc cggttttgcg
      421 ggggccattg gtggatatgc aaaaggagcc attctcgccg gagtcgcaac aacagcggtc
      481 tggctgaata ccacggacga taacctgacc gatcct
//
LOCUS       DAJTUP010000348          514 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTUP010000348 DAJTUP010000000
VERSION     DAJTUP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.356"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..514
                     /locus_tag="OO468_004283"
                     /pseudo
     CDS             1..514
                     /locus_tag="OO468_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          107 a          141 c          148 g          118 t
ORIGIN      
        1 gtggcttctg tttctatcag ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac
       61 ggcaaaagca ccgccggaca tcagcgctat ctctgctctc actgccgtaa aacatggcaa
      121 ctgcagttca cttacaccgc ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc
      181 atgaatggcg ttggatgccg ggcaaccgcc cgcattatgg gcgttggcct caacacgatt
      241 ttccgccatt taaaaaactc aggccgcagt cggtaacctc gcgcatacag ccgggcagtg
      301 acgtcatcgt ctgcgcggaa atggacgaac agtggggata cgtcggggct aaatcgcgcc
      361 agcgctggct gttttacgcg tatgacaggc tccggaagac ggttgttgcg cacgtattcg
      421 gtgaacgcac tatggcgacg ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg
      481 tgatatggat gacggatggc tggccgctgt atga
//
LOCUS       DAJTUP010000349          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTUP010000349 DAJTUP010000000
VERSION     DAJTUP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.357"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            76..>510
                     /locus_tag="OO468_004284"
     CDS             76..>510
                     /locus_tag="OO468_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR6261139.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQ"
BASE COUNT          140 a          117 c          133 g          120 t
ORIGIN      
        1 tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt ccgcattcca
       61 ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca acttgctgct
      121 gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact gcctgccgaa
      181 cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc catcatcatg
      241 ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt ggtttccaac
      301 cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta cggtccgttt
      361 gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc ggcaactcag
      421 gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg cggcgaacaa
      481 tgcttccgtg attccgagtg ggatttacag
//
LOCUS       DAJTUP010000350          494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTUP010000350 DAJTUP010000000
VERSION     DAJTUP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.358"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..418
                     /locus_tag="OO468_004285"
     CDS             <1..418
                     /locus_tag="OO468_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR6261140.1"
                     /translation="ITEALVERFARSTLQILLVNHINHANEVDETFRQAMAKLRRVGV
                     TLLNQSVLLRGVNDNAQTLANLSNALFDAGVMPYYLHVLDKVQGAAHFMVSDDEARQI
                     MRELLTLVSGYLVPKLAREIGGEPSKTPLDLQLRQQ"
BASE COUNT          124 a          113 c          137 g          120 t
ORIGIN      
        1 tatcaccgag gcgctggttg aacgctttgc ccgttctacg ctgcaaatct tgctggtgaa
       61 tcacatcaat catgccaatg aggtagatga aacgttccgt caggcgatgg ctaaattgcg
      121 ccgtgtcggt gtcaccctgc ttaaccagag cgttctgtta cgtggtgtga acgataacgc
      181 acaaacgttg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct
      241 gcatgtgcta gataaagtac agggcgcggc gcattttatg gtgagtgatg acgaagcacg
      301 gcagattatg cgtgagttgc tgacattggt gtcgggatat ctggtgccga aactggcgcg
      361 agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagtaact
      421 gattaaacag ggaagttaaa cattaaaatt gcctgatgcg ctacgcttat caggcctaca
      481 tcgtgcattg aaat
//
LOCUS       DAJTUP010000351          489 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTUP010000351 DAJTUP010000000
VERSION     DAJTUP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.359"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(23..>489)
                     /locus_tag="OO468_004286"
     CDS             complement(23..>489)
                     /locus_tag="OO468_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR6261141.1"
                     /translation="HKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQV
                     FVVILGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSY
                     TVGSLLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          143 a          137 c          110 g           99 t
ORIGIN      
        1 ttcacacttt gcttgaattc atttacacgt gagcaacggg tttcggcgta tgaccggcgt
       61 tgcgaagcag catcagcaaa tagataaacg atacgctggc gatcatgata aacaataaat
      121 tatcggagaa attctgcatt agcatagcgc taaatgacgg gccaagcaga cttcccacgg
      181 tatagctcaa cagtaaggct tggttcatcg ccaccagttg atgatgttca actttctcac
      241 aagcccaggc catcgccacc ggatatagcg taaagccagc ggcaccgagg atgaataacg
      301 ctggggccat cgccgcctgg ctaagcatcg caatactgcc gagaatgacg acaaagacct
      361 gaacacgcaa aaccagcagt cgaccaaact tatccgccag acgtccaatc ggccattgtc
      421 cgaggatacc cgcactgacc agtaccgcca tccagaaacc aatactggca ttgctcaccc
      481 ctttgtgat
//
LOCUS       DAJTUP010000352          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTUP010000352 DAJTUP010000000
VERSION     DAJTUP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.360"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(216..>488)
                     /locus_tag="OO468_004287"
     CDS             complement(216..>488)
                     /locus_tag="OO468_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR6261142.1"
                     /translation="QKLSFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILA
                     TGENVSLGTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT          142 a           98 c           92 g          156 t
ORIGIN      
        1 ttttaatcta ttatcgttaa acaatagatt aaatacgata agttaagcat catctctcat
       61 agtgtttaaa ttaacatatt ttccaatata aaaaataaga ctctggcttc aattgtgcgc
      121 ggattttctt acaggtgtag gataatagcc ttcacaaaat aattatccgc gcaattttcg
      181 tgaatggagc gcatagacta aggtcaaaat cacaattatt catagataaa agtgacacca
      241 atgactgact ggacggttcc ggctgaaacc ttgtttcctg tttgtccata ggttgcgctt
      301 aaacccagag gtactttgct tttatttact gtccccaggg agacattttc tcccgtagcc
      361 agtatttttc cattacgcat tagagtgaca cccactcccg atgcttttgt tgcatcaggt
      421 gctgtgttag caaacacttg tcggctactg tcagtggtgg cacccgaaag ataaaatgag
      481 agcttttg
//
LOCUS       DAJTUP010000353          485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTUP010000353 DAJTUP010000000
VERSION     DAJTUP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.361"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..485
                     /locus_tag="OO468_004288"
                     /pseudo
     CDS             1..485
                     /locus_tag="OO468_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          140 a          126 c          124 g           95 t
ORIGIN      
        1 tcagggcaaa acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct
       61 caggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct
      121 cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc gcattatcct
      181 gtggtcacac tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac
      241 cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat
      301 ggcatcagcc acggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc
      361 taccagatgg gacgctggct tgctggcagg ctcatgaaag agctggggct ggtcagctgt
      421 cagcagccga ctcaccggta taaacgtggt ggtcatgaac atgttgctat ccctaactac
      481 cttga
//
LOCUS       DAJTUP010000354          481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTUP010000354 DAJTUP010000000
VERSION     DAJTUP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.362"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(337..>481)
                     /locus_tag="OO468_004289"
     CDS             complement(337..>481)
                     /locus_tag="OO468_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR6261143.1"
                     /translation="KYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVLAYE
                     PKK"
BASE COUNT          129 a          136 c          103 g          113 t
ORIGIN      
        1 catgcgaaga agggactacc atccgagttg ttgaggttcg gagtggcagc cccaggattg
       61 atagaaatat ctcttggggc ttcatctttc caaacctcag agttaacctg aaaccggaaa
      121 gcttcaggca cccgccgtta tcttaccctt ccttagcaaa atagctgttt atttatacag
      181 tgagtcctgt ttcggacttc tgtcggcaaa actcatcatt gctcttaaaa cgaaatcgcc
      241 cgcagccatc actgaccgcg ggcgaaagtg taaagcaggt gccttaccat cctgacctga
      301 caaccggata tgcggggatt actccccgca caacggctac ttcttcggtt cgtaagcgag
      361 aacagccctg aactcaatat tacgttcctt caccgtaaac tcacacagcg agtctccggc
      421 cagaagcgta aatcccagta ccgtcaaaca cagcactatg actgccacaa gggcatattt
      481 c
//
LOCUS       DAJTUP010000355          481 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTUP010000355 DAJTUP010000000
VERSION     DAJTUP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.363"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
BASE COUNT          165 a           90 c          105 g          121 t
ORIGIN      
        1 cgtactctct ggccagttgg gccacgcagg cccctagctg gtaagatttt tcaataagat
       61 caagcttaaa ctccatagag tcatagagta agaggcacga atcctgcggg ggcgaagaga
      121 atctgacatt gatagagtcc attaacgtta taagaggact ctatcatcgc caatcatcag
      181 acattgaaat agacgacact gagatgacgc ttacaattct tttggtttct gtaataagta
      241 atctgacatc ccccgtgccc agaagaggag agggatatca agcccaaggg ttaataattg
      301 atactacggt taaaattgcc ttcagtaaag gaaacgtgat taaattcagg ggatttcaga
      361 gtgaaaacca tacaacatat taaaatctgt tcatttagct ataatacata caagaaagtg
      421 tccagttaga taatgttatc taaataacca cagataaaaa cgcacataat tggggggggg
      481 g
//
LOCUS       DAJTUP010000356          478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTUP010000356 DAJTUP010000000
VERSION     DAJTUP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.364"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            183..>478
                     /locus_tag="OO468_004290"
     CDS             183..>478
                     /locus_tag="OO468_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="catalyzes the formation of a meso-diaminopimelyl-
                     meso-diaminopimelyl crosslink; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR6261144.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYA"
BASE COUNT          122 a          109 c          127 g          120 t
ORIGIN      
        1 cgcttttctt taaacttctt tacattaggt tatgtaaaaa cgtatcggcg tttatatact
       61 gaagataagc ctgatgagta acaggcttgc tcgtcatact ttcgtgagta ttggcgttgt
      121 acaggcaagt cgtaaaataa cagcctggct attcagagta tgataaaaac agggggcaag
      181 ggatgttgct taatatgatg tgtggtcgtc ggctgtcggc aatcagtttg tgcctggccg
      241 taacattcgc tccactgttc aatgcgcagg ccgatgagcc tgaagtaatc cctggcgaca
      301 gcccggtggc tgtcagtgaa cagggcgagg cactgccgca ggcgcaagcc acggcaataa
      361 tggcggggat ccagccattg cctgaaggtg cggcagaaaa agcccgcacg caaatcgaat
      421 ctcaattacc cgcaggttac aagccggttt atcttaacca gcttcaactg ttgtatgc
//
LOCUS       DAJTUP010000357          478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTUP010000357 DAJTUP010000000
VERSION     DAJTUP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.365"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>478
                     /gene="rspA"
                     /locus_tag="OO468_004291"
     CDS             <1..>478
                     /gene="rspA"
                     /locus_tag="OO468_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR6261145.1"
                     /translation="EDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLI
                     DYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWVPNFGV
                     QEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLPVARL"
BASE COUNT          116 a          134 c          115 g          113 t
ORIGIN      
        1 ggaagacccg acgccagcgg aaaaccaaga atgcttccgt ctcattcgcc aacataccgt
       61 cacacccatc gcagtgggtg aagtcttcaa cagcatctgg gactgcaaac aactgattga
      121 agagcaactc atcgattata tccgcaccac gctgacccat gcaggcggaa ttaccggtat
      181 gcgccggatt gccgattttg cttcgctgta tcaggtacgt actggctcac acggtccttc
      241 cgatttgtca ccagtctgca tggctgcggc gctgcacttt gatctgtggg tccccaattt
      301 cggtgtccag gaatacatgg gttattccga acaaatgctc gaagtcttcc cgcacaactg
      361 gactttcgat aacggctata tgcatccggg agacaaaccg ggtcttggta tcgaattcga
      421 tgaaaagctg gcggcgaaat atccctatga acctgcttat ctaccagtcg cacgtctg
//
LOCUS       DAJTUP010000358          478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTUP010000358 DAJTUP010000000
VERSION     DAJTUP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.366"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..478
                     /locus_tag="OO468_004292"
                     /pseudo
     CDS             1..478
                     /locus_tag="OO468_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          178 a           83 c           86 g          131 t
ORIGIN      
        1 taaaactaac gcacaatatt aattctcagg ataagaaaac taatgcaaaa ttaattctgg
       61 atggtagtgt caacacaaaa aatgatgttg aagtcagtaa tgccagtctt accatgtaag
      121 gccatgcaac agagcatgca atattcagaa gcacagcgag tcattgctcc ctggtatttc
      181 tttgtggaac ggactgggtc accgttttga aagaaacaga gagttcatat aataaaaagt
      241 tcaattctga ttacaaaagt aataatcagc agacctcatt tgatcagcct gactggaaaa
      301 ccggggtgtt taaatttgat acattacacc tgaacaatgc tgacttttca atatcacgca
      361 atgccaatgt tgaaggaaat atatcagcaa ataaatcagc tatcacaatc ggcgataaaa
      421 atgcttacat tgataatctt gcagggaaaa atattactaa taatggtttt gacttcaa
//
LOCUS       DAJTUP010000359          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTUP010000359 DAJTUP010000000
VERSION     DAJTUP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.367"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            214..>474
                     /locus_tag="OO468_004293"
     CDS             214..>474
                     /locus_tag="OO468_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Stimulates the peptidyltransferase activity of the
                     70S ribosome and enhances dipeptide synthesis with
                     N-formylmethionyl-tRNA and puromycin in vitro, suggesting
                     its involvement in the formation of the first peptide bond
                     of a protein; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR6261146.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDK"
BASE COUNT          125 a          113 c          116 g          120 t
ORIGIN      
        1 atagggctgg aaattgaagg ggcagtgcaa cacgccccgc aacccggacg gcattccata
       61 cgttgttctc ttaaggttaa ccaccggatt gatgagcgcg cacagtacca ccttttttgc
      121 accagcaaaa gtgcgaatac cacttgcccg aaaggcccgt cgcgagtact ttgtcgcgat
      181 atttttgaca ttttcgacta caggaatttt tcgatgccaa gagcgaacga aattaaaaaa
      241 ggtatggtac tgaattacaa cggcaaactg ctgctggcta aggatattga tattcagtcg
      301 cccactgccc gcggtgccgc tacgctgtac aaaatgcgtt tttctgatgt ccgtaccggg
      361 ctgaaagtag aagagcgttt caaaggtgat gatatcgttg acaccgtgac gctgacccgc
      421 cgttacgttg acttctccta tgtcgatggc aacgaatatg tctttatgga taaa
//
LOCUS       DAJTUP010000360          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTUP010000360 DAJTUP010000000
VERSION     DAJTUP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.368"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            133..>474
                     /locus_tag="OO468_004294"
     CDS             133..>474
                     /locus_tag="OO468_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCR6261147.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVS"
BASE COUNT          121 a          102 c          141 g          110 t
ORIGIN      
        1 cccacagccg gatgaactaa cgttcgtccg gtttgctatt gagttcctgc ctggatactt
       61 accgcgaaaa cagcacggct gacataaaat aaatatggtc taatggggaa atttctcgtg
      121 gagagggaac agatgcgata tccggttgat gtatacacag gcaagattca ggcttatccc
      181 gaaggcaaac ccagcgcaat tgccaaaatc caggttgatg gtgagttgat gctgacggag
      241 ctggggctgg aaggtgacga gcaggcggag aaaaaggttc acggcgggcc agacagagcg
      301 ctgtgtcatt atcctcgtga gcactatctc tactgggtgc gggaatttcc ggaacaggcg
      361 gagttgtttg ttgcgcctgc gtttggcgaa aatctctcaa ccgacggtct cacggaaagt
      421 aatgttcata tgggcgatat tttccgctgg ggagaggcat taattcaggt cagc
//
LOCUS       DAJTUP010000361          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTUP010000361 DAJTUP010000000
VERSION     DAJTUP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.369"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..474
                     /locus_tag="OO468_004295"
                     /pseudo
     CDS             1..474
                     /locus_tag="OO468_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          137 a          101 c          120 g          116 t
ORIGIN      
        1 ctacttcact actgatgatg gttacaggat catctcggca cgttttggtg tcccccgaac
       61 ccaggtcagg acatgggttg ccctctatga aaaacatgga gaaaaaggtt taattcccaa
      121 acctaaaggc gttagtgctg atccagagtt gcgtattaag gtcgtgaaag ctgtgatcga
      181 gcagcacatg tcccttaatc aggctgctgc tcactttatg cttgctggta gtggttctgt
      241 agccaggtgg ctgaaggtct atgaagagcg cggagaagct ggtttacgcg cgctcaagat
      301 tggcaccaaa agaaacattg caatatcagt tgatccagaa aaagcggcat cagcattgga
      361 gctgtcaaaa gaccgacgca ttgaggatct tgaaaggcaa gttcgatttc ttgaaacgcg
      421 gcttatgtat ctaaaaaagc tgaaagcctt agctcatccc acgaaaaagt gaaa
//
LOCUS       DAJTUP010000362          474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTUP010000362 DAJTUP010000000
VERSION     DAJTUP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.370"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>474
                     /locus_tag="OO468_004296"
     CDS             <1..>474
                     /locus_tag="OO468_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR6261148.1"
                     /translation="ELRLRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPE
                     KPEIAHEIAQHLLGQKKLPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSA
                     TVLQEQGVAALPRFAPYAASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTK"
BASE COUNT          138 a          112 c           98 g          126 t
ORIGIN      
        1 ttgaattacg cctacgtaaa catttatcac tggcggaaga gtctctctgg caaaaatgtg
       61 cacaaaaact tattgccgca attccacata ttccagaatg gcggcaacca ttaattgctt
      121 tgttattacc cgaaaaacca gaaattgcac atgaaattgc ccagcatcta ctggggcaaa
      181 aaaaattacc ctcgcttgag tggttaaaaa tagtggcgac tgatgagcac attcttgcct
      241 cattagaaaa atatcacgag ccatatgcca tttttgatga ttactattgt ggtgcgatat
      301 ggtcagccac cgtattacag gagcaaggtg ttgcagccct gccccgattt gctccctatg
      361 ccgcaagtga ctactgcgcc gatgtgttgc gtcatatcaa tcatccgttc gcattaacac
      421 tgcttatacg tgtagccggg catactaaac gctgtcacga tcgaatgacg aaag
//
LOCUS       DAJTUP010000363          473 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTUP010000363 DAJTUP010000000
VERSION     DAJTUP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.371"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..473
                     /locus_tag="OO468_004297"
                     /pseudo
     CDS             1..473
                     /locus_tag="OO468_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          104 a          117 c          147 g          105 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgc
//
LOCUS       DAJTUP010000364          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTUP010000364 DAJTUP010000000
VERSION     DAJTUP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.372"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>472
                     /locus_tag="OO468_004298"
     CDS             <1..>472
                     /locus_tag="OO468_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR6261149.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAJTUP010000365          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTUP010000365 DAJTUP010000000
VERSION     DAJTUP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.373"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..281)
                     /locus_tag="OO468_004299"
     CDS             complement(<1..281)
                     /locus_tag="OO468_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR6261150.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMA"
BASE COUNT          135 a          105 c           98 g          132 t
ORIGIN      
        1 gccatcagac gttcacgccc ttctgcattc tgcacaggag cttccagttg cgtaataaac
       61 gaatcataac gttcgttatc cagcacaaat aaacgttgat ccaggataat gtttttagct
      121 tcctggtacg ccagttccat gatgaaatcg gtacggttct tattgaccaa gctcgcagca
      181 taatcaataa cggctcgttc ctcatcagtt gcccggatgt taatctgatg ttctcgcggt
      241 gttttacgga cgcttgcagc tgtagacata aaaacctcca ttggtaacaa tttgctatgc
      301 aattaatgtt atcgcaaatg gtaacagaac gctatacaga atcacttacg ggaaaagcga
      361 tcccctgata aaaacaatgg cagagacctg aacattgtga cgatgatctg tgtcggcgca
      421 caaatcatgt ctgattacgg ccgtttaatg tgattttgat aatgaaatgc
//
LOCUS       DAJTUP010000366          465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTUP010000366 DAJTUP010000000
VERSION     DAJTUP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.374"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(28..459)
                     /locus_tag="OO468_004300"
     CDS             complement(28..459)
                     /locus_tag="OO468_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR6261151.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT          138 a          121 c           88 g          118 t
ORIGIN      
        1 tttagtatct gacacaaagc tatcattcta acctttccgc tcgacggaaa tcatggtgac
       61 aaaaggatag cgttgccacg ggattgcccc gcctttcata tacatatgtg aaatcgtgcc
      121 accaagataa agcagctgct caacgttcag tttcgctttg gcataacagg caaaatcata
      181 aaaatttgtc gcctgctggc tcaacaaaaa cacggcattc ccatgtttat taatcccaac
      241 accgttacga attttacgtg aggcgacgtt gggatgaata cgcggattaa ttacaccgtt
      301 ttccatcaac attggtcctg actgcaccgc aaactgaata tctttactgg ctttaaaagc
      361 atccagacga acgatgccga ctttatctcc cgcgacataa aacacgccgc caggacggat
      421 aaagaaattc ccttcacctg aagcgagatt taacgccacc ttctg
//
LOCUS       DAJTUP010000367          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTUP010000367 DAJTUP010000000
VERSION     DAJTUP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.375"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
BASE COUNT          139 a           98 c          100 g          127 t
ORIGIN      
        1 tttgatacgc cacggtgacg ttcaccgcct gtgcctgaaa agcgatgaat gccagtgcgg
       61 caagaagtgt gttatatgac taggtaattt attcaagata accgggaatg ggtaaaaaaa
      121 gacgggattt gatgggattc aataagttat gcgccgattg cattcaagat aaaacgagtt
      181 aaaaatgctc aatgcttcga tatcgataaa acattcaaga taggcgcgcc gtgtatttta
      241 attcttttaa gactcttcaa aaatgtcatc gtagccttta aaaacgaatt ttaaaggcac
      301 cctaaaggca ggtcaatgaa gttaacccgc cttcgcgttt acactctctg agagaatact
      361 agcttcaatc tcttccaaaa tctctctgac ttgctcgtcg ggcatatcaa cgagcatcaa
      421 cgctgccagg cgtttacggc cctctagttg cagtagctct tgag
//
LOCUS       DAJTUP010000368          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTUP010000368 DAJTUP010000000
VERSION     DAJTUP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.376"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..236)
                     /locus_tag="OO468_004301"
     CDS             complement(<1..236)
                     /locus_tag="OO468_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6261152.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQA"
     gene            complement(246..>461)
                     /locus_tag="OO468_004302"
     CDS             complement(246..>461)
                     /locus_tag="OO468_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6261153.1"
                     /translation="GQTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGA
                     GLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          110 a          136 c          105 g          110 t
ORIGIN      
        1 gcctgctgaa attctttctg ccgctgagcc tgcttttcct gctgaaaaac acccgcaata
       61 cctgaaagac cttgcaaagc agtcagccca acattgttag cgcctgaacg ctcaatatca
      121 ttgttctgcc tgataagctg aagcgtattg ccgatgtcat ttacgctcgg agcgtttgag
      181 ttgacgccgc cgataccagc caacaatccg ccgtttgttc cttgccaagt agccatgatt
      241 cccccttaaa acaacgagcc aagcaatccg ataccagcac caataccagc gccccaaggc
      301 gttgatgttc ccaaaaggct ggcaagacct gcaccggcaa tcgcaccaga cgtgccaccg
      361 ctaattgcag tctgaagact tgatggttta ttggcattag cagcggcaag agctgcgctt
      421 tgctgtgcaa tgctgctcat gttgttggcg tacgtctgcc c
//
LOCUS       DAJTUP010000369          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTUP010000369 DAJTUP010000000
VERSION     DAJTUP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.377"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..236)
                     /locus_tag="OO468_004303"
     CDS             complement(<1..236)
                     /locus_tag="OO468_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR6261154.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQFIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQA"
     gene            complement(246..>461)
                     /locus_tag="OO468_004304"
     CDS             complement(246..>461)
                     /locus_tag="OO468_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR6261155.1"
                     /translation="GQTYANNMSSIAQQSAAIAAANANKPSSLQTAISGGTSGAIAGA
                     GLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          111 a          135 c          101 g          114 t
ORIGIN      
        1 gcctgctgaa attctttctg ccgctgagcc tgcttttcct gctgaaacac ccccgcaata
       61 cctgaaaggc cttgcaaagc agtcagccca acattgttag cgcctgaacg ctcaatatca
      121 ttgttctgcc tgataaactg aagcgtattg ccgatgtcat ttacgctcgg agcgtttgag
      181 tttacgccgc cgataccagc caacaatcca ccatttgatc cttgccaagt agccatgatt
      241 accccttaaa acaacgagcc aagcaatccg ataccagcac caatgccagc gccccaaggt
      301 gttgatgttc ccaaaaggct ggcaagacct gcaccggcaa tcgcaccaga cgtgccaccg
      361 ctaattgcag tctgaagact tgatggttta ttggcattag cagcggcaat tgctgcactt
      421 tgctgtgcaa tgctgctcat gttgttggcg tatgtctgcc c
//
LOCUS       DAJTUP010000370          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTUP010000370 DAJTUP010000000
VERSION     DAJTUP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.378"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..364
                     /locus_tag="OO468_004305"
     CDS             <1..364
                     /locus_tag="OO468_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium transporter TrkA"
                     /protein_id="HCR6261156.1"
                     /translation="VQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIE
                     AVAHGDESTSRVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTD
                     KKFITDVERLFQPSPFFL"
BASE COUNT          127 a           98 c          107 g          129 t
ORIGIN      
        1 ggttcagggg agcgtgatcg atattgcaat ttcaccacaa caagcaacta tttctgcgtt
       61 gcttagccat gtgcgaaaag cagatattgt tggtgtttcc tcattgcgcc gcggcgtagc
      121 agaagctatt gaagccgttg ctcacggtga tgaaagcacc tcacgcgttg tcggcagagt
      181 cattgacgaa atcaagctac cgccaggaac gattattgga gcggtggtac gtggaaacga
      241 cgtgatgatt gccaatgaca atctgcgcat tgagcaaggc gatcacgtaa ttatgttcct
      301 cacagataaa aagtttatta ccgacgtcga aagactcttc cagccaagcc ctttcttctt
      361 gtaattaata aggcgtctaa tgacgcctta ttatttccct ttgattatca aggattaatt
      421 aaattcattc ctggcaggaa aatggcttaa catttgttag a
//
LOCUS       DAJTUP010000371          461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTUP010000371 DAJTUP010000000
VERSION     DAJTUP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.379"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..255)
                     /locus_tag="OO468_004306"
                     /pseudo
     CDS             complement(1..255)
                     /locus_tag="OO468_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT          126 a           95 c          106 g          134 t
ORIGIN      
        1 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
       61 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
      121 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacgacatc
      181 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgggtga cgccttcctg
      241 cggcaccagt aatataaagc gcgcaaaatt attcaagata tttttgaaaa tcattcaaga
      301 taggctatcg cgtgtgatag caaaaacatt caagataaaa atgcttgtta attaacaaat
      361 gatatcttct atatattttt attgtttata gatggctatt tcggtaaaaa tgcattttca
      421 gaatatcaac cagcggtaaa tcgaagtaaa gggagtgaat t
//
LOCUS       DAJTUP010000372          456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTUP010000372 DAJTUP010000000
VERSION     DAJTUP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.380"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(110..>456)
                     /locus_tag="OO468_004307"
     CDS             complement(110..>456)
                     /locus_tag="OO468_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR6261157.1"
                     /translation="EYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKA
                     ELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQK
                     AAFSKQSIAKVD"
BASE COUNT          110 a          116 c           82 g          148 t
ORIGIN      
        1 cctgtgccat ttttcaggca taaaaaaacc gctcggcggc gggttttgct tactttacca
       61 tcgcgtacaa aatcggcaaa gtatcagatt tgcatgagat atatgccttt taatctactt
      121 ttgcaatact ttgctttgaa aatgccgctt tttgttctga acgtgttctc atcacaaaca
      181 ataaagcctc actatccagt cggtgaaaaa tgtgtttcat tacaacccag tgaccagtaa
      241 atgttttgga ccagtttttg gttgttactc ccgccagtaa tgccagctcc tgatattcat
      301 aaccttcccc accaaaaagt tctgctttta ccgcctgcgc cgccagccag attaattttt
      361 tcaggcgttc ctgtgttttc tctgcaattt tcctagtgcc agaatgagcc ttaaactctt
      421 cccacgccca gtgggttatg gtgatctgat actccc
//
LOCUS       DAJTUP010000373          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTUP010000373 DAJTUP010000000
VERSION     DAJTUP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.381"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(174..404)
                     /locus_tag="OO468_004308"
                     /pseudo
     CDS             complement(174..404)
                     /locus_tag="OO468_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT          141 a          114 c           83 g          112 t
ORIGIN      
        1 gtttatgcaa tatattgaat ttgcatggtc ttgtaggcca gataagacgt tcacgtcgca
       61 tccggcatga acaaagcgca ctttgtcaaa aatctaacct actttttaat tcaggaaatt
      121 accgcaaagc ccacgccatc atacccaacg taacaaaact caggcacgtt ccccactcgc
      181 cccgagaaaa atagcattaa tgcgcccagc gtcagcataa aaatattgag cagaaggtaa
      241 attgttggcg taaagagtaa attgcaaacc tcaggaatag cggcaagccc agcccccagc
      301 atggcaataa ccgctgcaaa aaattgcgtc agtattggga tgccgaaaaa agtcattacc
      361 agcgaggtca aaatccattt tctattttgc attattcttt ccattctttc tgaatggcga
      421 aattatactc cccgagtccc cttgcccctt
//
LOCUS       DAJTUP010000374          449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTUP010000374 DAJTUP010000000
VERSION     DAJTUP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.382"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
BASE COUNT          152 a           98 c           64 g          135 t
ORIGIN      
        1 atgcttctta tacacaatta gaaataaaac actcacaacc caaaaataga gcatccggca
       61 aaagagtgag ttaacatcat tgttaatcaa ttcgaccagt cagtaaatat gctgactggt
      121 cgaacataat ttgctaaaaa gtcatcactg actgatgtca gtcccaccat ctgccagttc
      181 aatctacggg tatttccagc atatcgcggc acgcgattgc tggtctgttg atgtagtgga
      241 ttgtagttgc tgacttttct taccctcaga tttctgcccc acttgtcaat tcaatctgcc
      301 aataaataac ataattatat ttttattcct cacagaaatt cctttttaaa aatagcgaac
      361 ccactgtaaa aaacactttt tgtatacctc taccaagcgt aataaaataa acgctcataa
      421 ttcattataa ataataatga aatgacgcc
//
LOCUS       DAJTUP010000375          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTUP010000375 DAJTUP010000000
VERSION     DAJTUP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.383"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>447)
                     /gene="virK"
                     /locus_tag="OO468_004309"
     CDS             complement(<1..>447)
                     /gene="virK"
                     /locus_tag="OO468_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR6261158.1"
                     /translation="PTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNLPEKYSKFLF
                     PQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTFSVILTQNGH
                     IAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACN"
BASE COUNT          140 a           99 c           87 g          121 t
ORIGIN      
        1 attgcaagct ttcatcaacg cacaaaatgc ttcaaaaata atccgttttg ggaaaatccc
       61 ataacaggcc cgggttgcgc accgaataac atcaggtccg gtatttttag gggctccctg
      121 taatccgcca ataaaagcaa tgtgtccatt ttgagtcaga atgactgaaa atgttaaccg
      181 ggcaataact gttttattgt aaaacaatga caacatcaac tctccttccc ggtcaaaacc
      241 actgggagaa cactggatat caaaattctc tccatctttc ccgatgaatt ggactaatga
      301 taccgatttt tgtggaaaca aaaatttaga atatttttct ggcaaatttt gcacaaagcg
      361 ataatgctca agaatatatt gtccacgggt ccaggcgcgc ccccctttat gcaaataagg
      421 gcgatgaatt cttgctggca acgtggg
//
LOCUS       DAJTUP010000376          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTUP010000376 DAJTUP010000000
VERSION     DAJTUP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.384"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..447
                     /locus_tag="OO468_004310"
     CDS             <1..447
                     /locus_tag="OO468_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6261159.1"
                     /translation="DEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLR
                     IVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGHGFGKWLLSDAIH
                     RVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV"
BASE COUNT           95 a           87 c          117 g          148 t
ORIGIN      
        1 gatgagcata tacttcagcc atttgactgt ggaaatgagg tgctaagtaa ctggttacgt
       61 ggtcgggcta tgaaaaacca gatgctcaat gcttctcgca cgttcgttat ttgcttggag
      121 gatactttac gtattgtggg atactactca ttagctactg gttccgtcac tcatgccgag
      181 cttggtcgca gtttgcggca taacatgccc aatcctgtcc ctgttgttct gttagggcgt
      241 ctggctgttg atgtttgtac ccaggggcat ggtttcggta aatggctact aagtgatgcc
      301 attcaccggg ttgtcaatct ggctgatcag gttggcatta aggctgttat ggtacatgca
      361 attgatgatg acgccagagc attttatgag cgttttggtt ttgttcagtc agttgtggca
      421 cctaatacct tgttttataa agtctga
//
LOCUS       DAJTUP010000377          444 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTUP010000377 DAJTUP010000000
VERSION     DAJTUP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.385"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..349
                     /locus_tag="OO468_004311"
     CDS             <1..349
                     /locus_tag="OO468_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6261160.1"
                     /translation="IQAQGILLQGQAELLKAENQQAQIQVEAAKVEAQNQLNAAKIAE
                     IFNNMDLDKQAELREYLRLVGQFQQQRSKDARANAELLLKDADQTHSQRMDFANLMRQ
                     VQIPSGGVAETPQ"
BASE COUNT          133 a          117 c          107 g           87 t
ORIGIN      
        1 aattcaggcg cagggtatcc ttctgcaagg tcaggctgaa ttgctcaagg cagagaacca
       61 acaggcgcag attcaggttg aagctgccaa ggttgaagcc caaaaccaac tcaacgccgc
      121 gaagattgca gaaatattca acaatatgga cctcgacaag caggcagaac tgcgtgagta
      181 cctcaggctc gtaggtcaat tccagcaaca gcgcagcaaa gatgctcgtg ctaacgctga
      241 gctgcttctt aaagatgcag accagactca ttcacaacgc atggatttcg cgaatcttat
      301 gcgtcaagtt caaatcccct ccggcggagt agccgagaca cctcaataag agagagttaa
      361 tcatggacca aaccaccgac attcaggctt ctgaagaatt aaccctgccc ggcaatcatg
      421 cagcggcatc tgctgatggc ttag
//
LOCUS       DAJTUP010000378          439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTUP010000378 DAJTUP010000000
VERSION     DAJTUP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.386"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(1..279)
                     /locus_tag="OO468_004312"
     CDS             complement(1..279)
                     /locus_tag="OO468_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261161.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
BASE COUNT           95 a          107 c          135 g          102 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgcc
//
LOCUS       DAJTUP010000379          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTUP010000379 DAJTUP010000000
VERSION     DAJTUP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.387"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            35..434
                     /locus_tag="OO468_004313"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO468_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTUP010000380          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTUP010000380 DAJTUP010000000
VERSION     DAJTUP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.388"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..347)
                     /locus_tag="OO468_004314"
     CDS             complement(<1..347)
                     /locus_tag="OO468_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR6261162.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENG"
BASE COUNT          104 a          109 c          105 g          112 t
ORIGIN      
        1 ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat gatggcatcc
       61 tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag ggtggtgaga
      121 cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga ctgccctgca
      181 catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc ttcagcgcgg
      241 ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg aggtgcagat
      301 gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt ctcttacctt
      361 tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga cgttaaccag
      421 ttaacaatta
//
LOCUS       DAJTUP010000381          430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTUP010000381 DAJTUP010000000
VERSION     DAJTUP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.389"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>430)
                     /locus_tag="OO468_004315"
     CDS             complement(<1..>430)
                     /locus_tag="OO468_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HCR6261163.1"
                     /translation="FGLRHRFRILNNKIINRVTGSEFVFYGLWRNIEEIKSLEGIDML
                     WLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVTDFVWRNFVVDPPEGTLIRKIN
                     YDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESDDDAA"
BASE COUNT          117 a          115 c           93 g          105 t
ORIGIN      
        1 gccgcatcat catcagactc cggaacgccc tcatacacat gtttaaaacc atccggatca
       61 cggcgtcgcg ccgcgtcgat aaccttaagc atggtgtcag acagaaacgg attttcgtca
      121 tagttaattt tgcggatgag agtgccttcg ggcggatcaa caacgaagtt gcgccagacg
      181 aaatcagtaa caagtccggg gttgaatatg aaccagcatt ccgaaccctc tttacggatc
      241 gttggctcca gaattttcca ctggtattcc gtcagtgcgt gggcttcttc caaccacaac
      301 atatcgatcc cctccagtga cttaatttct tcgatgttgc gccataatcc ataaaaaaca
      361 aattccgagc cagtaacccg gttaatgatt ttgttgttca gaatacggaa acggtgccgc
      421 agaccaaacc
//
LOCUS       DAJTUP010000382          426 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTUP010000382 DAJTUP010000000
VERSION     DAJTUP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.390"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            130..>426
                     /locus_tag="OO468_004316"
     CDS             130..>426
                     /locus_tag="OO468_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261164.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIP"
BASE COUNT          132 a           93 c           88 g          113 t
ORIGIN      
        1 aaataggttc agttgcagaa cgttttgcga accattttcc agttctgata tttagccatt
       61 tagcctatag aatcgcactc cgcctctctg ttagtttgct aataattaaa ttattacaaa
      121 ggtgatcaaa tggaatggca acaattgctt aactgcaacc gtcggaaaga taatctaaaa
      181 aaaaccaaca tagcattttt acatcccctt gctagcggaa gacaagagat cgagcgggat
      241 tacgaccgat tactttttgc tgcaccaact cgtcggcttg cggataaaac acaagttttc
      301 ccgttagatc aaaatgacag catacggacg aggctgactc attctcacga agtagcaaac
      361 tttgctcgcg ggataggaat gaggctcgct ttcgagatga aggagcacat ctttggcgaa
      421 ataccc
//
LOCUS       DAJTUP010000383          424 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTUP010000383 DAJTUP010000000
VERSION     DAJTUP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.391"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..302
                     /locus_tag="OO468_004317"
     CDS             <1..302
                     /locus_tag="OO468_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR6261165.1"
                     /translation="DNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
BASE COUNT           76 a          111 c          107 g          130 t
ORIGIN      
        1 tggataacgc ccacgttcag gtcacctttc tggtggagaa actctccccc gcatggcaac
       61 gacgagtcgc tctggttacc cattccttaa tcttatttat ttgtggcgca ctggcctggg
      121 gcgcaacgct aaaaacaatt caggactgga gcgattattc accgatcctc ggtttgccca
      181 tcggcctgat gtatgccgct tgtctgccca ccagcctcgt tatcgcattc tttgaactgc
      241 gtcatttata tcaactcatc acgcgtagca attctctaac gtcgccaccg caaggagctt
      301 aatcatggct gtgctgattt ttctgggctg tctgttgggt ggtattgcta tcggtttgcc
      361 tattgcctgg gcactgttgt tgtgcggggc ggcgttaatg ttctggctgg acatgtttga
      421 tgtc
//
LOCUS       DAJTUP010000384          422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTUP010000384 DAJTUP010000000
VERSION     DAJTUP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.392"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>422)
                     /locus_tag="OO468_004318"
     CDS             complement(<1..>422)
                     /locus_tag="OO468_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6261166.1"
                     /translation="PLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQL
                     EGVKHSQRGSKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVN
                     KALRVMGYDTKVEVCGHGFRTMACSSLIESGLWSRDAV"
BASE COUNT          107 a          132 c           82 g          101 t
ORIGIN      
        1 ctaccgcatc cctcgaccat aatcccgact caatcaacga actacaagcc atcgtcctga
       61 agccatgccc acaaacctcg accttcgtgt cataccccat tacgcgcaaa gccttgttca
      121 ccgtattctc actcatcggc ttacgtggat cgtgatcgcc cacaaaaatc agctctcgat
      181 ttccgctcat gcttttgatc ttttccaaaa tacttagggc ctgccgcgac aaaggtacaa
      241 ggtgtggtgt ccgcatcttc gagccacgct gagaatgctt gactccttcc agttgctcac
      301 gctcacctgg gattgtccac atagccgttt caaaatctat ttctgaccag cgggcaaaac
      361 gcagctcgct cgaacggatg aaaaccaata aagtgagttc aaccgccagt cgagttaacg
      421 gt
//
LOCUS       DAJTUP010000385          421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTUP010000385 DAJTUP010000000
VERSION     DAJTUP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.393"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>421
                     /locus_tag="OO468_004319"
     CDS             <1..>421
                     /locus_tag="OO468_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6261167.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMD"
BASE COUNT           95 a          136 c          114 g           76 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 t
//
LOCUS       DAJTUP010000386          418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTUP010000386 DAJTUP010000000
VERSION     DAJTUP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.394"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
BASE COUNT          118 a          111 c           82 g          107 t
ORIGIN      
        1 ttgatgcggt gcctggtgcc tccaggtgac gttaaccagt taacaattac agttggcttt
       61 cccacccaaa ccaataagga ctaacatgac ttttaactgt gccgcgtgcg catagccgca
      121 ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagccagcaa ttaaaccgat
      181 gccgccaact ggtacttcac acagcaatgt cgttatttac aaccggaagc gcactcccac
      241 catttaaatt tcacagacaa gaccgactct ttatggatac cggaaatgcg ccttcgtgtt
      301 gtgcccggtt ttatttcacc acttccgggc tttggtggta tctttactga agttctcaca
      361 caaccagtaa ggaaatgaat atgccaacgt atctcgccag agtagaacta tataatgc
//
LOCUS       DAJTUP010000387          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTUP010000387 DAJTUP010000000
VERSION     DAJTUP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.395"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>413
                     /locus_tag="OO468_004320"
     CDS             <1..>413
                     /locus_tag="OO468_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR6261168.1"
                     /translation="REVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFSPRLCR
                     PFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVANQRKH
                     ETIQARPCDRWLEEQQSMLALPPEKKEYDVHPGEN"
BASE COUNT          101 a          114 c          113 g           85 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg atggtgcagt
      181 acacccgtaa cagtttttac atcccactaa tgactcgcct gcgcccgatg gggagcactg
      241 tcgatgttga aacagccaac cgccacggtc tgcgctggct gcacgatgtc gctaaccaac
      301 gaaagcatga aacaatccag gcccgtccct gtgatcgctg gctcgaagag cagcagtcca
      361 tgctggcact gcctccggag aaaaaagagt atgacgtgca tcctggtgaa aat
//
LOCUS       DAJTUP010000388          411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTUP010000388 DAJTUP010000000
VERSION     DAJTUP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.396"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            1..411
                     /locus_tag="OO468_004321"
                     /pseudo
     CDS             1..411
                     /locus_tag="OO468_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT           96 a          124 c          105 g           86 t
ORIGIN      
        1 ggcaatctac atagcctgcg aacgtggatc aaagagcgcg gtcaggatta ccccgcccag
       61 acactcacca cacatctgtt tattcctctg cgccgacggc ttcagtgcca acaaccgact
      121 ctccaggcgc tgctggcgat cctcgacggc gtactgatca actacatcgc catttgtctg
      181 gcttcggcac gtaaaaaaca gggtaaagat gcgctggtgg ttggctggaa tattcaggat
      241 accacccgtc tgtggctgga gggctggatt gccagtcaac aaggatggcg cattgatgtc
      301 ctcgctcgcc cactcgctca atcaactacg ccctgaacta ttcgaaggcc gtacattgct
      361 ggtgtggtgc ggtgaaaatc gaacctccgc ccaacagcag caactcacca g
//
LOCUS       DAJTUP010000389          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTUP010000389 DAJTUP010000000
VERSION     DAJTUP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.397"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            39..405
                     /gene="tagK"
                     /locus_tag="OO468_004322"
                     /pseudo
     CDS             39..405
                     /gene="tagK"
                     /locus_tag="OO468_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT          120 a           96 c           91 g           98 t
ORIGIN      
        1 ctggttgtct ggagattcag ggggccagtc tatacacaat gaaaccaaat attacgtggg
       61 gactccaaaa aatacaggcg cacgggatcg ccgataacac aatttacgcg actgaaggaa
      121 cacaaattat cttcaccgtt acctctccct ggattcctgt ttttaaggtg aatgatgatg
      181 taaagattgc cctcactctg acccgccacg aagaagcatg gtggattatc aatcagtcta
      241 ccgaatattg ctgtacggta aacgaccaga ttgtggaacc tcatcaccgt atgcgtctga
      301 atgaaggcga cctgatagaa tgggggttat cttcatagtg ccttgtacat ggcaatccat
      361 catctccgaa cctgagcaca gtaaaaacga tatcgcagac tgcca
//
LOCUS       DAJTUP010000390          403 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTUP010000390 DAJTUP010000000
VERSION     DAJTUP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.398"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(169..>403)
                     /locus_tag="OO468_004323"
     CDS             complement(169..>403)
                     /locus_tag="OO468_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR6261169.1"
                     /translation="TGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPA
                     QTAVLGGTFTDNKQCTLSEARHQLPITPFRMPS"
BASE COUNT          108 a          119 c           91 g           85 t
ORIGIN      
        1 ataacgacta ccagaaaagc acttcgcatc actcagacat gatttaacat aatatacatt
       61 atgcgtagta atattgtagt agctaaattg agatgtccgt ctctgactaa atagacatgt
      121 ccgcgctaca gtctcaccac agcgatcact gctttcaaag gatgggactc atgacggcat
      181 acggaacgga gtaatcggca actgatgccg ggcctcgctg agcgtgcact gcttattatc
      241 cgtgaacgtg ccgccaagaa ccgcggtttg tgccggacgc acaacgccta acccaaggca
      301 accaagctgc acgtcgcctc ctccaacgac caccagagtg cccgccttca gaccgcagag
      361 ttccgccgct tgtgaactta ccacgcccag caatgtgccg gtt
//
LOCUS       DAJTUP010000391          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTUP010000391 DAJTUP010000000
VERSION     DAJTUP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.399"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..337
                     /locus_tag="OO468_004324"
     CDS             <1..337
                     /locus_tag="OO468_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR6261170.1"
                     /translation="TEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDKVIRLVR
                     GQSYRGSRLGPVGISVPGTPTGTYIASPQFFITGCSEHSLPGSYCALSGVPDAHVSGA
                     MPGLFIRTS"
BASE COUNT           98 a           95 c          109 g           93 t
ORIGIN      
        1 cacagaagat attatttaca gaatcgtggc gagctctgtc cgcaacagat ggggagaccc
       61 tgacattggc gggctgatta ttgctgcgta ccagggagaa gctgacggtg ataaagtcat
      121 cagacttgtc agggggcagt catacagagg ctcacgactg ggaccggtgg ggatttcagt
      181 gcccggtact cccaccggaa cgtatatagc atccccacaa tttttcatta cgggatgttc
      241 agagcattca ttaccggggt catattgcgc cctgtccggg gtgccggatg cacatgtctc
      301 tggcgcaatg cccgggcttt ttattcgcac atcgtgagga atgcaccgtg gaaattaaaa
      361 aaatcattaa tccccgttat accgaaagtg gcgca
//
LOCUS       DAJTUP010000392          393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTUP010000392 DAJTUP010000000
VERSION     DAJTUP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.400"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>393)
                     /locus_tag="OO468_004325"
     CDS             complement(<1..>393)
                     /locus_tag="OO468_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
                     /protein_id="HCR6261171.1"
                     /translation="LDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCGFFLYPLKFS
                     FIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGWF
                     LLIAISTLTTWQHHFIDVITGLAVGMLI"
BASE COUNT          137 a          100 c           87 g           69 t
ORIGIN      
        1 caatcaacat acctaccgcc agccctgtga tgacatcaat aaaatgatgc tgccatgtcg
       61 tcagcgtcga aatggcgatg agtaaaaacc atccgccaca gactttacgc cacctcacag
      121 ccagatgctg acgaaagtga cgccaaagta gccagcagag aataatatgc agcgaaggag
      181 actggttata aggcagatca aacagttcaa gttgcgaaaa tagccatcct gtcaccccac
      241 tcacttcagg acggataaaa ctaaacttca gcgggtagag aaaaaaaccg cagcaggcca
      301 ttaccgttgc cagaataagc cggtggacaa gtcggcgttg ttcgaatgtc gagctacaaa
      361 cgaacagcga aaatccatat aaaagatcca gac
//
LOCUS       DAJTUP010000393          387 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTUP010000393 DAJTUP010000000
VERSION     DAJTUP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.401"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..358)
                     /locus_tag="OO468_004326"
     CDS             complement(<1..358)
                     /locus_tag="OO468_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR6261172.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNL"
BASE COUNT           94 a           88 c          101 g          104 t
ORIGIN      
        1 gtaaattact gtccgggaca acaggaatac gagacagcag gacccgattc ccaggcaggg
       61 atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag
      121 ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg gcaatgttaa
      181 ccgctgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg ttggcggcat
      241 atcttgctcc cgccaggtac tctgcttttg cggaatacca gagctggatg ttcaaaatag
      301 cgacaccggt aataaacaag catatcgcaa cgaggatggg tatgagtgta ttgcgcattt
      361 gcttgtcccg gccaaagggt ttaatac
//
LOCUS       DAJTUP010000394          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTUP010000394 DAJTUP010000000
VERSION     DAJTUP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.402"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>385
                     /locus_tag="OO468_004327"
     CDS             <1..>385
                     /locus_tag="OO468_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCR6261173.1"
                     /translation="DEVCEAHLATLLEQQPHGIAARLRARGEQVAFLGLLDTWPPETQ
                     NWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGNYADAVRLLTTAHSV
                     PFDGKATLFVAERTLQEGMSPERAWS"
BASE COUNT           86 a          100 c          123 g           76 t
ORIGIN      
        1 ggatgaagtc tgcgaagcgc atctggcaac gctacttgag caacaaccgc acggcattgc
       61 ggcgcggctg cgtgcccgtg gcgaacaggt ggcatttctt ggtttgctgg atacctggcc
      121 gccagagacg caaaactggc aggaaaaaga ggctaatggt ctggacccgg aagtgctggc
      181 ggagattaac cgcgaacgcg aggccttcct ggcggcacag cagggaagta cttcaacgga
      241 gttgtttacc accattgaag gcaactacgc tgatgctgtg cgcctgctga cgactgctca
      301 tagcgtaccg tttgatggta aagcgacgct gtttgttgct gaacgcacgc ttcaggaagg
      361 tatgagcccc gaacgcgcct ggtcg
//
LOCUS       DAJTUP010000395          383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTUP010000395 DAJTUP010000000
VERSION     DAJTUP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.403"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
BASE COUNT          113 a           93 c           73 g          104 t
ORIGIN      
        1 acagacctgt gctatcggaa agaaaaaagg ccatcagcaa aactctgatg acctgaatca
       61 cctttaccaa tattgtatga aaaaacacgc acgactaatt gacaataatt ttcatttcca
      121 ttaaaaaaga tcgcgtagca ttcttaattc atgagggact ttacccgcca gcaatctgag
      181 tagcgaaagc tgttcatccc caatgttttt gctggcgggt ccttttttct acggtcctcc
      241 tccccggagg ggggcatttc attatttttc taatcgttac tggtgagaag gcggaagaaa
      301 aagccagttc ttcgccccta aataacagca tgattccagt ccatgcaaaa tctgatttcc
      361 agctcacaca taccgagcgc tgt
//
LOCUS       DAJTUP010000396          381 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTUP010000396 DAJTUP010000000
VERSION     DAJTUP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.404"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..212)
                     /locus_tag="OO468_004328"
     CDS             complement(<1..212)
                     /locus_tag="OO468_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR6261174.1"
                     /translation="MTTVTVHCPRCNSDEVYRHGRSCSRHERFRCRSCKRVFQLTYSY
                     EARKLGVKEQIVEMAHNGAGGRDTART"
BASE COUNT          111 a           85 c           93 g           92 t
ORIGIN      
        1 gtcctggcgg tatcacggcc ccctgcgccg ttatgtgcca tttcaacgat ctgctcctta
       61 acgccgagtt ttctggcttc ataagagtaa gtgagctgga aaacacgttt acatgagcga
      121 caacggaaac gctcatgccg ggaacaacta cggccatgtc gataaacttc atcggaatta
      181 cagcgcggac aatgaacagt aacggtagtc atggaaaacc tcaaatacca gtagattacc
      241 agaaacttct atcgattgaa gacattacct tttgccgcaa taggtggggt aacattggtg
      301 aaaacgcatt acgccgggtg gcagttggaa aaaaattatc ttttttcggc tctgatcgtg
      361 gtggagaaaa tgcggcaata a
//
LOCUS       DAJTUP010000397          377 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTUP010000397 DAJTUP010000000
VERSION     DAJTUP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.405"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>377)
                     /locus_tag="OO468_004329"
     CDS             complement(<1..>377)
                     /locus_tag="OO468_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR6261175.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLG"
BASE COUNT          100 a          119 c           87 g           71 t
ORIGIN      
        1 accaagattg aggcgaatac cgccccagta gagcaggaag ccgagcacca cggcagtagc
       61 aagaccgagc attgcaccca gcggcggcca gatcccgaca tcttgttgaa atgccgccag
      121 caggaaaaag accgactcca gcccttccct tgcaacggca aaaaagacca tcatcaccag
      181 cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg actgcctgtt ccagttgcac
      241 tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc caggtaagga tcaccacggc
      301 gatcaccgcc acgatacctt caaacagttc ctgctctttt tgcggaaatt cgccggtggt
      361 ttcgttaatg aagatcc
//
LOCUS       DAJTUP010000398          376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTUP010000398 DAJTUP010000000
VERSION     DAJTUP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.406"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>376
                     /locus_tag="OO468_004330"
     CDS             <1..>376
                     /locus_tag="OO468_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR6261176.1"
                     /translation="NGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVT
                     YTDAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPAD
                     KMIPGDGRTLVVHFDKFGNVEFT"
BASE COUNT          102 a          102 c           88 g           84 t
ORIGIN      
        1 aatggtcgcg ctgtcactac gtctgttgcg gttgcggagt tcttccggaa aatgcacaaa
       61 aacgttattc agaagatcga aactctcgaa tgctcgccgg agtttaaccg gcttaatttt
      121 aagcccgtca cctataccga cgccaaaggc gaaaaacgcc caatgtacca aatcaccaaa
      181 aacggcttcg ttttcctggt gatgggcttc actggcaaaa aagccgccgc attcaaggaa
      241 gcctacattg ctgagttcga ccgcatggag gccgaactgc gccagaataa caccccacct
      301 gcagacaaga tgattccggg tgatggtcgc actctggttg ttcacttcga caaattcggc
      361 aatgtcgaat tcaccg
//
LOCUS       DAJTUP010000399          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTUP010000399 DAJTUP010000000
VERSION     DAJTUP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.407"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..68)
                     /locus_tag="OO468_004331"
     CDS             complement(<1..68)
                     /locus_tag="OO468_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR6261177.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPS"
     gene            complement(78..>373)
                     /locus_tag="OO468_004332"
     CDS             complement(78..>373)
                     /locus_tag="OO468_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261178.1"
                     /translation="DVRRELWKALLGWMPDPQGGEIVYVGGTLLDLNRYELYYQFDFT
                     AKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEHHLEMRFPQN"
BASE COUNT           92 a          107 c           89 g           85 t
ORIGIN      
        1 tcggaaggca aaaggtctcc acgggcaggg tcaggaaccg accgcccttt caggggtttc
       61 acaaacatga gggactctta attctgaggg aaacgcattt ccagatggtg ctcaatgtca
      121 ccatccgggc cagtaccagg atcgatgtaa tccacatcaa tactcagcag ggataaatcc
      181 ggcagggcat tcacgtcctc tgcctgtcgc gtgtcttctt ccgtgatttc atacttcgcc
      241 gtaaaatcaa actggtaata cagttcgtaa cggttcagat ccagcagggt gccaccgaca
      301 taaacaatct cgcccccttg cgggtccggc atccatccaa gcagggcttt ccagagttct
      361 ctccggacat cat
//
LOCUS       DAJTUP010000400          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTUP010000400 DAJTUP010000000
VERSION     DAJTUP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.408"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            20..>372
                     /locus_tag="OO468_004333"
     CDS             20..>372
                     /locus_tag="OO468_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6261179.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFG"
BASE COUNT           95 a           76 c           92 g          109 t
ORIGIN      
        1 cgcatcgcta aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac ttattgacag
       61 acaaagtggt gaactgattg gcatctgtac ttttacggca tattcgctgg aaagacaacg
      121 cgtttctggc gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca gattagtcat
      181 gttgggggga gcgcggaagt atcaaaagcg gggctttggt caggacctac tatgtgattt
      241 ttttgaacat gtaaaaaaaa ttcacccggc attaccaatt aaaggggttt atcttgatgc
      301 tgaccctgcc gccattaact tttatgctcg tctcggcttt gttcagcttt cagcgacacc
      361 aaatgctttt gg
//
LOCUS       DAJTUP010000401          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTUP010000401 DAJTUP010000000
VERSION     DAJTUP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.409"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..311)
                     /locus_tag="OO468_004334"
     CDS             complement(<1..311)
                     /locus_tag="OO468_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6261180.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTUP010000402          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTUP010000402 DAJTUP010000000
VERSION     DAJTUP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.410"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(273..>371)
                     /locus_tag="OO468_004335"
     CDS             complement(273..>371)
                     /locus_tag="OO468_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR6261181.1"
                     /translation="DTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          104 a           83 c           77 g          107 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtggg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt c
//
LOCUS       DAJTUP010000403          366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTUP010000403 DAJTUP010000000
VERSION     DAJTUP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.411"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            86..>366
                     /locus_tag="OO468_004336"
     CDS             86..>366
                     /locus_tag="OO468_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCR6261182.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINR"
BASE COUNT           88 a           96 c           88 g           94 t
ORIGIN      
        1 gattatcatg accctgcctg tgctgtttgt gacgctggct gcgctggcgc tacgtctctc
       61 tttcactttg taatgagata ctgatatgag caacattacc atttatcaca acccggcctg
      121 cggcacgtcg cgtaatacgc tggagatgat ccgcaacagc ggcacagaac cgaccattat
      181 ccattatctg gaaactccgc caacgcgcga tgaactggtc aaactcattg ccgatatggg
      241 gatttccgta cgcgcgctgc tgcgtaaaaa cgtcgaaccg tatgaggagc tgggccttgc
      301 ggaagataaa tttactgacg atcggttaat cgactttatg cttcagcacc cgattctgat
      361 taatcg
//
LOCUS       DAJTUP010000404          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTUP010000404 DAJTUP010000000
VERSION     DAJTUP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.412"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>365
                     /locus_tag="OO468_004337"
     CDS             <1..>365
                     /locus_tag="OO468_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR6261183.1"
                     /translation="KPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQP
                     IVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNR
                     ARGRHQITAMSEIVRELSQ"
BASE COUNT          113 a           88 c           76 g           88 t
ORIGIN      
        1 aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa tgattacaac
       61 ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga aatagatggt
      121 tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat cgtggatggt
      181 tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt aaaaggttat
      241 ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac tattcgtcat
      301 aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg tgaacttagc
      361 cagtt
//
LOCUS       DAJTUP010000405          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTUP010000405 DAJTUP010000000
VERSION     DAJTUP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.413"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..161
                     /locus_tag="OO468_004338"
     CDS             <1..161
                     /locus_tag="OO468_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR6261184.1"
                     /translation="FAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKRYR
                     KKLTEFNQ"
     gene            266..>361
                     /locus_tag="OO468_004339"
     CDS             266..>361
                     /locus_tag="OO468_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261185.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELP"
BASE COUNT          110 a           61 c           83 g          107 t
ORIGIN      
        1 attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt gcgtttgagg
       61 ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg tcctttacgc
      121 ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata cctgacaatt
      181 cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg ccagatatca
      241 gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca ctattggtgg
      301 cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc acgaattgcc
      361 g
//
LOCUS       DAJTUP010000406          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTUP010000406 DAJTUP010000000
VERSION     DAJTUP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.414"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>359
                     /locus_tag="OO468_004340"
     CDS             <1..>359
                     /locus_tag="OO468_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR6261186.1"
                     /translation="QQAQQQPQQPSAEQIQAQGILLQGQAELLKAENQQAQIQVEAAK
                     VEAQNQLNAAKIAEIFNNMDLDKQAELREYLKLVGQFQQQRSKDARANAELLLKDADQ
                     THSQRMDFANLMRQVQTP"
BASE COUNT          109 a          101 c           87 g           62 t
ORIGIN      
        1 cagcaggctc aacagcagcc gcagcagcca tcagcagagc aaattcaggc gcagggtatc
       61 cttctgcaag gtcaggctga attgctcaag gcagagaacc aacaggcgca gattcaggtt
      121 gaagccgcca aggttgaagc tcaaaaccaa ctcaacgccg cgaagattgc agaaatcttc
      181 aacaatatgg acctcgacaa gcaggcagaa ctgcgtgagt acctcaagct cgtaggtcaa
      241 ttccagcaac agcgcagcaa agacgctcgc gctaacgctg agctgcttct taaggatgca
      301 gaccagactc attcacaacg catggatttc gcgaatctta tgcgtcaagt tcaaacccc
//
LOCUS       DAJTUP010000407          356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTUP010000407 DAJTUP010000000
VERSION     DAJTUP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.415"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..216
                     /locus_tag="OO468_004341"
     CDS             <1..216
                     /locus_tag="OO468_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HCR6261187.1"
                     /translation="VYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRY
                     ALLDYIHETTIRSLDAYPVHSEHQNLN"
BASE COUNT          116 a           64 c           69 g          107 t
ORIGIN      
        1 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
       61 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgggtac
      121 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
      181 tatcctgtac actcagaaca tcagaattta aattaaggtt tgatgtattc tctgaataaa
      241 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
      301 gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta catggt
//
LOCUS       DAJTUP010000408          350 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTUP010000408 DAJTUP010000000
VERSION     DAJTUP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.416"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..226)
                     /gene="lysO"
                     /locus_tag="OO468_004342"
     CDS             complement(<1..226)
                     /gene="lysO"
                     /locus_tag="OO468_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR6261188.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLC"
BASE COUNT          132 a           66 c           85 g           67 t
ORIGIN      
        1 tacacagtaa aataacggta atactgacgg cagaataatg cagaatcgcc aacaggttac
       61 tggcgaggtt atcgagaaac gccagactga tacccataaa aaagagaata aggtaaacca
      121 tccagcttaa tagctgatta atagctttta acgcagcttg ttggcgaagc ggaatgaggt
      181 aacccacaat caggggaacc agaatgatta acagcccaga aaacatgaaa atccagtcct
      241 tgcaaagaag aagtcgaaat gcgcgatgac acactactga aagcggaagg gcgagtaaag
      301 ttgcaattaa aaggaaatgt tatgcatcag agggggattg gatttgcccc
//
LOCUS       DAJTUP010000409          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTUP010000409 DAJTUP010000000
VERSION     DAJTUP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.417"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..237
                     /locus_tag="OO468_004343"
     CDS             <1..237
                     /locus_tag="OO468_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR6261189.1"
                     /translation="PRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQR
                     PLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
BASE COUNT           89 a           80 c           92 g           88 t
ORIGIN      
        1 ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc gcagcttacc
       61 gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa cggtaaatcc
      121 attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt ctttggtagg
      181 atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt ctcttaatct
      241 tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac atcaacttaa
      301 tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatc
//
LOCUS       DAJTUP010000410          345 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTUP010000410 DAJTUP010000000
VERSION     DAJTUP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.418"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..305
                     /locus_tag="OO468_004344"
     CDS             <1..305
                     /locus_tag="OO468_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR6261190.1"
                     /translation="YVLNQWDALCYYSDDGLAEADNNTAERALRAVCLGKKNYVFFGS
                     DHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDDLLPWKVVLPSG"
BASE COUNT           76 a           84 c          104 g           81 t
ORIGIN      
        1 gctatgtact gaatcagtgg gacgccctct gttattacag tgatgacggt ctggcggagg
       61 cggacaataa cacagcggaa agagcgcttc gtgcagtctg tctcggaaag aaaaattacg
      121 tgttcttcgg tagcgatcac ggcggcgagc gtggtgcact gctgtacggg ctgatcggca
      181 cctgccgtct gaacggtatc gatccggaag cgtatctgcg ccatattctg agcgtactgc
      241 cggaatggcc ttccaaccga gttgacgacc tcctgccatg gaaggtggtg ctaccatcag
      301 gataatcgtc aatacggtgt tcggtgaacg tttacttgga gcatc
//
LOCUS       DAJTUP010000411          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTUP010000411 DAJTUP010000000
VERSION     DAJTUP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.419"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            34..>344
                     /locus_tag="OO468_004345"
     CDS             34..>344
                     /locus_tag="OO468_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-binding ATPase"
                     /protein_id="HCR6261191.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     I"
BASE COUNT           99 a           89 c           89 g           67 t
ORIGIN      
        1 ctgatggttt atctatacaa aaaaggagtc gggatgaacg aacaatccca ggccaaatca
       61 ccagaagcct tgcgcgcaat ggtcgccggg acactggcca attttcagca cccaaccctg
      121 aagcataacc tcaccacgct gaaagcgttg caccatgtcg cctggatgga cgacacgttg
      181 catgtggaac tggttatgcc gtttgtctgg catagcgcgt tcgaagagtt aaaagagcaa
      241 tgtagcgccg aactgctgcg catcaccggg gcgaaagcta tcgactggaa actgtcgcat
      301 aacatagcca ctctgaaacg cgtgaagaat cagccgggaa ttaa
//
LOCUS       DAJTUP010000412          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTUP010000412 DAJTUP010000000
VERSION     DAJTUP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.420"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO468_004346"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO468_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR6261192.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTUP010000413          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTUP010000413 DAJTUP010000000
VERSION     DAJTUP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.421"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>344
                     /locus_tag="OO468_004347"
     CDS             <1..>344
                     /locus_tag="OO468_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR6261193.1"
                     /translation="AWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALE
                     ALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRW
                     NAAVKRAGIRRR"
BASE COUNT          107 a           69 c           91 g           77 t
ORIGIN      
        1 tggcgtggga ggatgtggat ttcgagaagg gaattgtgaa tgtcagaaga aacctgacga
       61 tacttgatat gttcggtccc ccaaaaacaa atgccgggat ccggacggta gcattactgc
      121 agccggctct tgaagcactg aaggagcaat acaaactgac cgggcatcat cgcaaaagcg
      181 aaatcacttt ttatcatcgg gagtacggca gaactgaaaa acaaaaactg cattttgttt
      241 tcatgcccag ggtgtgtaac gaaaaacaga aaccttatta ctcggtaagc agtttgggta
      301 caagatggaa tgcagcagta aaacgtgctg gtattcgccg ccgt
//
LOCUS       DAJTUP010000414          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTUP010000414 DAJTUP010000000
VERSION     DAJTUP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.422"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..246
                     /locus_tag="OO468_004348"
     CDS             <1..246
                     /locus_tag="OO468_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR6261194.1"
                     /translation="CGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKA
                     EHLEARKAMMQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT          100 a           79 c           84 g           79 t
ORIGIN      
        1 tgcggtcacg gcttccgggc aatggcatgc agtgcgctga tggaatcggg tttatgggca
       61 aaggacgcag tagaacgcca gatgagtcat caggagcgca ataccgtgcg catggcttat
      121 attcataagg cagagcacct ggaagcccgc aaagcgatga tgcagtggtg gtcggattat
      181 ctggaagcat gccgagaatc ttatgcaccg ccttatacaa ttggtaaaaa taagtttatc
      241 ccatagtata ctaacgaatc ccacactctg gcaaattacc accacctcta caggaggtgg
      301 ttctatatag acttttatca acttcatgac gaaaaacacc ta
//
LOCUS       DAJTUP010000415          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTUP010000415 DAJTUP010000000
VERSION     DAJTUP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.423"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>340
                     /locus_tag="OO468_004349"
     CDS             <1..>340
                     /locus_tag="OO468_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6261195.1"
                     /translation="YLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGV
                     MFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYV
                     SFSEAAHAIT"
BASE COUNT           82 a           85 c          108 g           65 t
ORIGIN      
        1 cgtacctcgc cgttgttctc gacctgttcg caagaaaacc agtgggctgg gccatgtcgt
       61 tctcgccgga cagcaggctc accatgaaag cgctggaaat ggcatgggaa acccgtggta
      121 agcccggcgg ggtgatgttc cacagcgatc agggcagtca ttatacgagc aggcagttcc
      181 ggcagttatt gtggcgatac cagatcagac agagtatgag ccggcgcgga aactgctggg
      241 ataacagccc aatggaacgc ttcttcagga gtctgaagaa cgaatggatg ccggtggtgg
      301 gttacgtaag cttcagcgag gcagctcacg ccataacgga
//
LOCUS       DAJTUP010000416          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTUP010000416 DAJTUP010000000
VERSION     DAJTUP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.424"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..170)
                     /locus_tag="OO468_004350"
     CDS             complement(<1..170)
                     /locus_tag="OO468_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261196.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..>339
                     /locus_tag="OO468_004351"
     CDS             246..>339
                     /locus_tag="OO468_004351"
                     /inference="COORDINATES: protein motif:HMM:NF013765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261197.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFY"
BASE COUNT          107 a           93 c           60 g           79 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttacc
//
LOCUS       DAJTUP010000417          336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTUP010000417 DAJTUP010000000
VERSION     DAJTUP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.425"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>336
                     /locus_tag="OO468_004352"
     CDS             <1..>336
                     /locus_tag="OO468_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR6261198.1"
                     /translation="SSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLK
                     EAGLLASTGSTGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRR
                     LLERLGHTPP"
BASE COUNT           91 a           82 c           98 g           65 t
ORIGIN      
        1 atcgtccatg gaaacgacat tcgtgctgga tgcactggag caggcgttat gggcccgtcg
       61 accgtccggc acggtccatc acagtgataa aggttctcag tatgtatcgc tggcctacac
      121 acagcggctt aaggaagccg gattactggc atcaacagga agtacaggcg actcgtatga
      181 caacgcgatg gcggagagca tcaatggtct ttacaaagcg gaggtaatac accgtaagag
      241 ctggaaaaac cgtgcagaag tggaactggc cacactcacg tgggtggact ggtataacaa
      301 tcgacgattg ctggaaaggc tgggccatac tcctcc
//
LOCUS       DAJTUP010000418          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTUP010000418 DAJTUP010000000
VERSION     DAJTUP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.427"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>335)
                     /locus_tag="OO468_004353"
     CDS             complement(<1..>335)
                     /locus_tag="OO468_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR6261199.1"
                     /translation="DIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KQGPYEFICT"
BASE COUNT           84 a           95 c           88 g           68 t
ORIGIN      
        1 gtacagatga actcatacgg cccctgtttg gcgatttcat ccgccagata agcggcaacc
       61 tggtaagagg cgttctgtac tttggtatag ccttcacgac cgaggcgcag gaattcatag
      121 tactgtgcaa ttacctgacc cgccgggcgg gagaagttga tggcaaaagt accaatttga
      181 ccacccaggt agtcaacgtt gaacaccagt tcctgcggca gcgcttcttc gtcacgccag
      241 ataacccagc cgcagcccag cggagccaga ccgaatttat ggcctgaagc actgatcgat
      301 ttcacacgcg gcaggcggaa gtcccagacg atatc
//
LOCUS       DAJTUP010000419          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTUP010000419 DAJTUP010000000
VERSION     DAJTUP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.428"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            51..>332
                     /locus_tag="OO468_004354"
     CDS             51..>332
                     /locus_tag="OO468_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR6261200.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPD"
BASE COUNT           83 a           70 c           98 g           81 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg ac
//
LOCUS       DAJTUP010000420          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTUP010000420 DAJTUP010000000
VERSION     DAJTUP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.429"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..176)
                     /locus_tag="OO468_004355"
     CDS             complement(<1..176)
                     /locus_tag="OO468_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6261201.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNT"
     gene            complement(151..>330)
                     /locus_tag="OO468_004356"
     CDS             complement(151..>330)
                     /locus_tag="OO468_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR6261202.1"
                     /translation="HLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITFDNV
                     ALQIRTDHAPLDFTK"
BASE COUNT          104 a           67 c           79 g           80 t
ORIGIN      
        1 gtgttggttc ccggtaccct ggtcaggtaa gaaatgttat aagccatctt cccgaaagat
       61 tcaataatca acaggttatc gcttactata catacagcgt tgaagaaaaa taaggcagat
      121 ataaaaaagg ctccacgagg gagcctgata ttattttgtg aaatccagcg gcgcatggtc
      181 agtgcgtatt tgcagcgcga cgttatcaaa ggtgatcata gtcgatttac aaaaaataca
      241 cttagcgccc aaagggtttc gctccgtcac atcgaatgcc gatgtacggt actgtgaacc
      301 atgacaacat ggacagcgga aatggagatg
//
LOCUS       DAJTUP010000421          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTUP010000421 DAJTUP010000000
VERSION     DAJTUP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.430"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(179..>330)
                     /locus_tag="OO468_004357"
     CDS             complement(179..>330)
                     /locus_tag="OO468_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261203.1"
                     /translation="DPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTTTKML
                     FMCRE"
BASE COUNT          115 a           79 c           53 g           83 t
ORIGIN      
        1 tgctgcaaac tcgaaacgac gcccgtaaaa acaaaactaa aaaatataac gtcgatattc
       61 aaattaagta aatgtcaacc gacggggact atccccgtca tagacgttaa cgccccactc
      121 ctctctgtcg ctcaagaggt taatgacgaa tagcagagaa cataatgtaa acaaggcatc
      181 actcacgaca cataaataac atctttgtgg tggtttgatt gatttttcca ctccgcttac
      241 catctacacc aaaataacga tcatcacgac ataatttttc tttaacatcg caaaatacac
      301 cgttagcgaa tgtaaattcg ctgggatcgt
//
LOCUS       DAJTUP010000422          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTUP010000422 DAJTUP010000000
VERSION     DAJTUP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.432"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(150..>323)
                     /gene="pflA"
                     /locus_tag="OO468_004358"
     CDS             complement(150..>323)
                     /gene="pflA"
                     /locus_tag="OO468_004358"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="activates pyruvate formate-lyase 1 under anaerobic
                     conditions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR6261204.1"
                     /translation="DMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPKKETMERV
                     KGILEQYGHKVMF"
BASE COUNT           88 a           71 c           69 g           95 t
ORIGIN      
        1 aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat atggtagatg
       61 cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa aaagcggcaa
      121 ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc gtactgctca
      181 agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcgagtttg
      241 tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg gagaagttcg
      301 attttctcaa cgttacccat atc
//
LOCUS       DAJTUP010000423          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTUP010000423 DAJTUP010000000
VERSION     DAJTUP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.433"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>322)
                     /locus_tag="OO468_004359"
     CDS             complement(<1..>322)
                     /locus_tag="OO468_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR6261205.1"
                     /translation="EVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPT
                     LCNGRNVAASLPDDYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMI
                     ITGVR"
BASE COUNT           84 a           78 c           94 g           66 t
ORIGIN      
        1 gacgaacacc agtgatgatc atgaagcgaa cagcattctt ctggtgtaca gaggtgcagg
       61 cagcaacatt gagccagagt cgggcgattg attcaatatc ggtaaaaagc cgtgtggggg
      121 taggtttctg tacgcgggag gaaacataat catctggcag actggcggca acattgcggc
      181 cgttgcaaag agtaggtgcg cagaacttcc agaaccgacg gagctcggca aacaactcca
      241 gggcgttatt gttcgagcga gtggcgatcc actcgtccag cacttccacc agccgattgt
      301 atgttacgtc gctgaacacc tc
//
LOCUS       DAJTUP010000424          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTUP010000424 DAJTUP010000000
VERSION     DAJTUP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.434"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(31..89)
                     /locus_tag="OO468_004360"
                     /pseudo
     CDS             complement(31..89)
                     /locus_tag="OO468_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           70 a           95 c           72 g           82 t
ORIGIN      
        1 gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta cgagtaacaa
       61 ccaaccagta tgatgacgaa cttcatcata accctttcct tctataaggc cccttcctcg
      121 ggaggggctt tcccgtttca gccccttgct gactccccga ttgttgatga gcgccggacc
      181 ccgcgcaacg ctttccctga agcagttttg ccttccccga gcggtcacgc aaaacgctgc
      241 aacgtcctga gcatttgcac cttttagcgg aacgcagata taaaaaatcc ccggcttttt
      301 gggccaggga tatgtgtta
//
LOCUS       DAJTUP010000425          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTUP010000425 DAJTUP010000000
VERSION     DAJTUP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.435"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..236)
                     /locus_tag="OO468_004361"
     CDS             complement(<1..236)
                     /locus_tag="OO468_004361"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261206.1"
                     /translation="MNDFLSEISRFPVTTTESFFNYCFVVFGVIQKINILDSSQSGVK
                     NGADSEAVLAALIEHLELINPSGRLSQNTRSAML"
BASE COUNT           95 a           71 c           61 g           90 t
ORIGIN      
        1 tgaagcattg ccgaacgggt attttggctt aatctgccag aaggattgat caattctaaa
       61 tgttcaataa gcgcagccaa aaccgcctca ctatccgccc catttttaac gcctgactgg
      121 ctactgtcta aaatatttat tttctgaatc acaccaaaaa caacgaaaca ataattaaaa
      181 aaggattctg ttgttgttac cgggaaccga cttatttcgc tcagaaagtc gttcaaccag
      241 ctccgcagtg ataagattcg ctgatgatac agtgtatcct cactgcgtgc tggaggtgcc
      301 agtataatat gttgatc
//
LOCUS       DAJTUP010000426          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTUP010000426 DAJTUP010000000
VERSION     DAJTUP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.436"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(44..315)
                     /locus_tag="OO468_004362"
                     /pseudo
     CDS             complement(44..315)
                     /locus_tag="OO468_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          106 a           34 c           59 g          116 t
ORIGIN      
        1 ctggggaaaa caaattaaat aacatgagct gcatagtagg tcaattgttc ttcccagaaa
       61 catttaaaaa atttaagttt ttgctctgtt ttattatggg gaataaagtt ccaatatcgg
      121 atgtattatt agtatcaatg tgggttattt tattattgtc cagtcttaaa cttttgatag
      181 atggaagata agtcaggaaa tcaagattta tatatttcaa ctggttgtta gcagcgttta
      241 atgtctgaac attgcggaag ttgctataga atattttaat ttcagaaagc aaattgttgt
      301 taataagtaa cgtat
//
LOCUS       DAJTUP010000427          313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTUP010000427 DAJTUP010000000
VERSION     DAJTUP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.437"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..>313)
                     /locus_tag="OO468_004363"
     CDS             complement(<1..>313)
                     /locus_tag="OO468_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR6261207.1"
                     /translation="VEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKVI
                     IMDEPTDALTDTETESLFRVIRELKSQGHCIVYISHRMKEIFEICDDVTVFRDGQFIA
                     ER"
BASE COUNT           80 a           77 c           74 g           82 t
ORIGIN      
        1 cgcgctcagc aataaattgc ccatcacgaa aaacggtaac gtcatcgcaa atctcgaaga
       61 tttctttcat gcggtgggag atatagacaa tacagtggcc ttgcgatttc agctcgcgga
      121 tgacgcggaa cagggattcg gtttcggtat cggtcagcgc atcggtcggt tcatccataa
      181 tgatgacttt cgactcaaag ctcagcactt tggcgatttc aaccatttgc tggtcaccga
      241 tggaaagatc gcccactaac ttgtcgcttt taaagcgcag gttaagttta gccagcaatt
      301 tatccgcttc gac
//
LOCUS       DAJTUP010000428          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTUP010000428 DAJTUP010000000
VERSION     DAJTUP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.438"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>312
                     /locus_tag="OO468_004364"
     CDS             <1..>312
                     /locus_tag="OO468_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR6261208.1"
                     /translation="GIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMASVTGNIYA
                     DDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSMNNAIWHLN
                     S"
BASE COUNT           92 a           72 c           72 g           76 t
ORIGIN      
        1 gggaatccct gaacagaata gtaaaactgt aacgccgggt ctccactacg ctgctgatgg
       61 attcaggctg agtggtggaa atgcaaattt cattgccaga aatatggcat ctgtcaccgg
      121 aaatatttat gctgatgatg cagcaaccat tactctggga cagcctgaaa ctgaaacacc
      181 gactatatcg tctgcttatc aggcatgggc agagactctt ttgtatggct ttgataccgc
      241 ctatcgaggc gcaataacag cccccaaagc tacagttagc atgaataatg cgatctggca
      301 tctaaatagc ca
//
LOCUS       DAJTUP010000429          312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTUP010000429 DAJTUP010000000
VERSION     DAJTUP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.439"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
BASE COUNT          107 a           68 c           54 g           83 t
ORIGIN      
        1 tgagattatc cgattaaggt gaagcagaaa aagcacgcta ccgccccagg ctcaacagct
       61 acctgaatac tgataccgca ctgatattga cgcgctagtg tatcacttct ttcgcggcaa
      121 tccgacccga atgattttta agcacattgt tgattttcaa taaagaaatc aatcaaataa
      181 aatcattagc aaccatattc tttcactgtt gctctgcgat tccttcctgc acgtacagca
      241 gaaatcaatg aatcgcataa aaatacaaaa aaagtataga tgttcaacaa gaattggcgg
      301 gtaaattgct at
//
LOCUS       DAJTUP010000430          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTUP010000430 DAJTUP010000000
VERSION     DAJTUP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.440"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>310
                     /locus_tag="OO468_004365"
     CDS             <1..>310
                     /locus_tag="OO468_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR6261209.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     "
BASE COUNT           73 a           80 c           77 g           80 t
ORIGIN      
        1 gcttgagcta ctctcacttc aacaacgacc tgtctgcaac catgagcaac ggtacttacg
       61 ttgacggtag caccaactcc gacgcttggg gcttcggttt gaaaaccggt tacgacttca
      121 aactgggtga tgctggttac gtgactcctt acggcagcat ttctggtctg ttccagtctg
      181 gtgatgacta ccagctgagc aacgacatga aagttgacgg tcagccttac gacagcatgc
      241 gttatgaact gggtgtagat gcaggttata ccttcaccta cagcgaagat caggctctga
      301 ctccgtactt
//
LOCUS       DAJTUP010000431          310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTUP010000431 DAJTUP010000000
VERSION     DAJTUP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.441"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
BASE COUNT          104 a           52 c           73 g           81 t
ORIGIN      
        1 taaggatagg tgacatcgaa aaagcgtgca gaggaatgtg tgcttgtgtc tcagcccaac
       61 gtgagtgtct gtttttgagt cgttgagttc tacagtccaa agtgctgaaa ccattcaaga
      121 gtcaattggc gcgcattata cgctgaaatt aatccaaaag gaaaacaaaa acagcgtttc
      181 aaaaaatata caacttgtta gacaataaat tgttatcaaa gatttaacgt tccagggaaa
      241 aggttacgca aaaacttcgc tgtttgcgca acgtctggtg ccgaagttgt cagtgtgagg
      301 ataagtgaaa
//
LOCUS       DAJTUP010000432          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTUP010000432 DAJTUP010000000
VERSION     DAJTUP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.442"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>304
                     /locus_tag="OO468_004366"
     CDS             <1..>304
                     /locus_tag="OO468_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR6261210.1"
                     /translation="IPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIF"
BASE COUNT           48 a           84 c           92 g           80 t
ORIGIN      
        1 gattccgttc tttgtgctgg cgggtgaaat catgaatgcg ggcggcttgt taaaacgcat
       61 tgttgacctg ccgatgaaac tggtggggca taaaccaggc gggctgggct acgtcggcgt
      121 gctggcagct atgattatgg cgagcctttc tggttctgcc gttgcggata ccgccgccgt
      181 tgccgctcta ctggtgccga tgatgcgcag cgccaactac ccggtcaacc gggcggcggg
      241 gctgattgcc tctggcggca ttatcgcgcc aattattccc ccttccattc cgtttattat
      301 cttc
//
LOCUS       DAJTUP010000433          300 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTUP010000433 DAJTUP010000000
VERSION     DAJTUP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.443"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..>300
                     /locus_tag="OO468_004367"
     CDS             <1..>300
                     /locus_tag="OO468_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR6261211.1"
                     /translation="YRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTIFDGVSLY
                     ASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSLNLSI"
BASE COUNT           94 a           63 c           63 g           80 t
ORIGIN      
        1 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgcaattggg
       61 gctataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
      121 gggtaagtct gtatgcctcc ggttcacagc aagactattg gggcaataac gagaaaaaca
      181 gtaatatctc tgttggggtt tccggccagc aatggggaat tggttacagc ctgaattatc
      241 aatacagccg ctacactgat gaaaataatg accgtgcact ctctttgaat ctcagtattc
//
LOCUS       DAJTUP010000434          298 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTUP010000434 DAJTUP010000000
VERSION     DAJTUP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.denovo.444"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            <1..296
                     /locus_tag="OO468_004368"
     CDS             <1..296
                     /locus_tag="OO468_004368"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HCR6261212.1"
                     /translation="KEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLPAFHDDQTT
                     LGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSKLNN"
BASE COUNT           91 a           64 c           77 g           66 t
ORIGIN      
        1 caaaagaaaa atgggctgaa cgcattgcat tactgcgtga ttatcaggtg aacagcaaga
       61 tgatgcagtt gaccggtaac ccggaggtca aattcctcca ttgcctgccc gcgtttcatg
      121 acgaccaaac gacgcttggc aaaaaaatgg cggaagagtt tggcctacat ggcggaatgg
      181 aagtgactga tgaggtcttc gaatctgccg ccagcattgt atttgatcag gcggaaaacc
      241 gcatgcatac catcaaagcg gtgatggtcg cgacgctcag taaattaaat aattaata
//
LOCUS       DAJTUP010000435         8793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.guided.138, whole genome shotgun sequence.
ACCESSION   DAJTUP010000435 DAJTUP010000000
VERSION     DAJTUP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.guided.138"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            264..923
                     /locus_tag="OO468_004369"
     CDS             264..923
                     /locus_tag="OO468_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6261213.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="OO468_004370"
     CDS             complement(1124..1501)
                     /locus_tag="OO468_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6261214.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="OO468_004371"
     CDS             complement(1568..4534)
                     /locus_tag="OO468_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6261215.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5097)
                     /locus_tag="OO468_004372"
     CDS             complement(4537..5097)
                     /locus_tag="OO468_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6261216.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5223..5573)
                     /locus_tag="OO468_004373"
     CDS             complement(5223..5573)
                     /locus_tag="OO468_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6261217.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="OO468_004374"
     CDS             complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="OO468_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6261218.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6999..7829
                     /locus_tag="OO468_004375"
     CDS             6999..7829
                     /locus_tag="OO468_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6261219.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7942..8733
                     /gene="aadA1"
                     /locus_tag="OO468_004376"
     CDS             7942..8733
                     /gene="aadA1"
                     /locus_tag="OO468_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6261220.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         2001 a         2357 c         2482 g         1953 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5101 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5161 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5221 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5281 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5341 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5401 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5461 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5521 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5641 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5701 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5761 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5821 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5881 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5941 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     6001 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6061 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6121 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6181 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6241 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6301 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6361 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6421 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6481 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6541 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6601 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6841 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6901 gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat taattgttag
     6961 ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca atacatatca
     7021 acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag
     7081 atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg
     7141 atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg
     7201 caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag
     7261 atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca
     7321 atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc
     7381 aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag
     7441 acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct
     7501 taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc
     7561 cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata
     7621 atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa
     7681 acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg
     7741 cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga
     7801 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
     7861 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
     7921 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
     7981 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8041 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8101 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8161 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8221 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8281 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8341 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8401 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8461 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8521 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8581 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8641 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     8701 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     8761 cgcttcgcgg cgcggcttaa ctcaagcgtt aga
//
LOCUS       DAJTUP010000436         8790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.guided.140, whole genome shotgun sequence.
ACCESSION   DAJTUP010000436 DAJTUP010000000
VERSION     DAJTUP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.guided.140"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            264..923
                     /locus_tag="OO468_004377"
     CDS             264..923
                     /locus_tag="OO468_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR6261221.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="OO468_004378"
     CDS             complement(1124..1501)
                     /locus_tag="OO468_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR6261222.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="OO468_004379"
     CDS             complement(1568..4534)
                     /locus_tag="OO468_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR6261223.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5097)
                     /locus_tag="OO468_004380"
     CDS             complement(4537..5097)
                     /locus_tag="OO468_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR6261224.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5223..5573)
                     /locus_tag="OO468_004381"
     CDS             complement(5223..5573)
                     /locus_tag="OO468_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR6261225.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="OO468_004382"
     CDS             complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="OO468_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR6261226.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6999..7829
                     /locus_tag="OO468_004383"
     CDS             6999..7829
                     /locus_tag="OO468_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR6261227.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7942..8730
                     /gene="aadA1"
                     /locus_tag="OO468_004384"
     CDS             7942..8730
                     /gene="aadA1"
                     /locus_tag="OO468_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6261228.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         1998 a         2357 c         2482 g         1953 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5101 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5161 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5221 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5281 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5341 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5401 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5461 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5521 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5641 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5701 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5761 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5821 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5881 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5941 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     6001 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6061 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6121 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6181 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6241 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6301 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6361 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6421 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6481 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6541 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6601 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6841 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6901 gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat taattgttag
     6961 ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca atacatatca
     7021 acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag
     7081 atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg
     7141 atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg
     7201 caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag
     7261 atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca
     7321 atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc
     7381 aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag
     7441 acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct
     7501 taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc
     7561 cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata
     7621 atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa
     7681 acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg
     7741 cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga
     7801 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
     7861 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
     7921 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
     7981 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8041 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8101 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8161 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8221 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8281 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8341 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8401 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8461 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8521 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8581 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
     8641 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
     8701 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
     8761 ttcgcggcgc ggcttaactc aagcgttaga
//
LOCUS       DAJTUP010000437         6161 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.guided.172, whole genome shotgun sequence.
ACCESSION   DAJTUP010000437 DAJTUP010000000
VERSION     DAJTUP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.guided.172"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            59..1102
                     /locus_tag="OO468_004385"
     CDS             59..1102
                     /locus_tag="OO468_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR6261229.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /locus_tag="OO468_004386"
                     /pseudo
     CDS             1164..1778
                     /locus_tag="OO468_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="OO468_004387"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="OO468_004387"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR6261230.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="OO468_004388"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="OO468_004388"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR6261231.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="OO468_004389"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="OO468_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR6261232.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="OO468_004390"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="OO468_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR6261233.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..>6161)
                     /locus_tag="OO468_004391"
     CDS             complement(5846..>6161)
                     /locus_tag="OO468_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR6261234.1"
                     /translation="YSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKG
                     KDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDTVAVGMTY
                     QF"
BASE COUNT         1494 a         1516 c         1633 g         1518 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgaatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata g
//
LOCUS       DAJTUP010000438         2996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.guided.220, whole genome shotgun sequence.
ACCESSION   DAJTUP010000438 DAJTUP010000000
VERSION     DAJTUP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.guided.220"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            complement(<1..597)
                     /gene="tetR(B)"
                     /locus_tag="OO468_004392"
     CDS             complement(<1..597)
                     /gene="tetR(B)"
                     /locus_tag="OO468_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR6261235.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEK"
     gene            679..1884
                     /gene="tet(B)"
                     /locus_tag="OO468_004393"
     CDS             679..1884
                     /gene="tet(B)"
                     /locus_tag="OO468_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR6261236.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1997..2590)
                     /gene="tetC"
                     /locus_tag="OO468_004394"
     CDS             complement(1997..2590)
                     /gene="tetC"
                     /locus_tag="OO468_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR6261237.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2678..>2996
                     /locus_tag="OO468_004395"
     CDS             2678..>2996
                     /locus_tag="OO468_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR6261238.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFD"
BASE COUNT          809 a          548 c          613 g         1026 t
ORIGIN      
        1 tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct ctgcaccttg
       61 gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag tagtaggtgt
      121 ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac ctaaagtaaa
      181 atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt gttggcataa
      241 aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg tacctaaatg
      301 tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag cgttattacg
      361 taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt gcctatctaa
      421 catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc aatacaatgt
      481 aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt cgattccgac
      541 ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc tagacatcat
      601 taattcctaa tttttgttga cactctatca ttgatagagt tattttacca ctccctatca
      661 gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat tacgttactc
      721 gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg tgaatttatt
      781 gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc gttaatgcag
      841 gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg cccagtgctg
      901 ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc aagtgcgctt
      961 tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg ggctgtcgcg
     1021 gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt cggttggtta
     1081 ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt tgcaggagag
     1141 atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcgc tttccttgtg
     1201 gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac cgaagtaggg
     1261 gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc cattttgttg
     1321 attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg ggtgctattt
     1381 accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc gggtcttggt
     1441 cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa atggggcgaa
     1501 aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt tttagcgttt
     1561 atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg tgggatcgct
     1621 ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca aggtgcttta
     1681 cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt actgtttgct
     1741 gttatttata atcattcact accaatttgg gatggctgga tttggattat tggtttagcg
     1801 ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca agctcagggg
     1861 agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt ccgcgaaata
     1921 taatgaccct cttgataacc caagagggca ttttttacga taaagaagat ttagcttcaa
     1981 ataaaaccta tctattttat ttatctttca agctcaataa aaagccgcgg taaatagcaa
     2041 taaattggcc ttttttatcg gcaagctctt ttaggttttt cgcatgtatt gcgatatgca
     2101 taaaccagcc attgagtaag tttttaagca catcatcatc ataagcttta agttggttct
     2161 cttggatcaa tttgctgaca atggcgttta ccttaccagt aatgtattca aggctaattt
     2221 tttcaagttc attccaacca atgataggca tcacttcttg gatagggata aggtttttat
     2281 tattatcaat aatataatca agataatgtt caaatatact ttctaaggca gaccaaccat
     2341 ttgttaaatc agtttttgtt gtgatgtagg catcaatcat aattaattgc tgcttataac
     2401 aggcactgag taattgtttt ttatttttaa agtgatgata aaaggcacct ttggtcacca
     2461 acgcttttcc cgagatctca tctattgaaa cagcttgata gcctttttca acaaacaata
     2521 ttcgtgctga gttaaccagt gattgatagg tactcttaaa attttcttgt tgatgatttt
     2581 tattttccat gatagattta aaataacata ccgtcagtat gtttatggta tcatgatgat
     2641 gtggtcgtga caatcttaag aacatttagg ttattttatg tatattgaac agcattctcg
     2701 ctatcaaaat aaagctaata acatccaatt aagatatgat gataagcagt ttcatacaac
     2761 ggttatcaaa gatgttctat tatggattga acataattta gatcagtctt tactgcttga
     2821 tgatgtggcg aataaagcgg gttataccaa gtggtatttt cagcggctgt tcaaaaaagt
     2881 aacaggggtc acactggcta gctatattcg tgctcgtcgt ttgacgaaag cggctgttga
     2941 gttgaggttg acgaaaaaaa ctatccttga gatcgcatta aaatatcaat ttgatt
//
LOCUS       DAJTUP010000439         2003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.guided.239, whole genome shotgun sequence.
ACCESSION   DAJTUP010000439 DAJTUP010000000
VERSION     DAJTUP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.guided.239"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            2..475
                     /locus_tag="OO468_004396"
     CDS             2..475
                     /locus_tag="OO468_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6261239.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            570..1094
                     /gene="sat2"
                     /locus_tag="OO468_004397"
     CDS             570..1094
                     /gene="sat2"
                     /locus_tag="OO468_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6261240.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1152..1943
                     /gene="aadA1"
                     /locus_tag="OO468_004398"
     CDS             1152..1943
                     /gene="aadA1"
                     /locus_tag="OO468_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6261241.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          545 a          474 c          503 g          481 t
ORIGIN      
        1 tgtgaaacta tcactaatgg tagctatatc gaagaatgga gttatcggga atggccctga
       61 tattccatgg agtgccaaag gtgaacagct cctgtttaaa gctattacct ataaccaatg
      121 gctgttggtt ggacgcaaga cttttgaatc aatgggagca ttacccaacc gaaagtatgc
      181 ggtcgtaaca cgttcaagtt ttacatctga caatgagaac gtattgatct ttccatcaat
      241 taaagatgct ttaaccaacc taaagaaaat aacggatcat gtcattgttt caggtggtgg
      301 ggagatatac aaaagcctga tcgatcaagt agatacacta catatatcta caatagacat
      361 cgagccggaa ggtgatgttt actttcctga aatccccagc aattttaggc cagtttttac
      421 ccaagacttc gcctctaaca taaattatag ttaccaaatc tggcaaaagg gttaacaagt
      481 ggcagcaacg gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg ccaggtttgc
      541 gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg
      601 cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat
      661 ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct
      721 cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg
      781 tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg
      841 tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt
      901 gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg
      961 cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata
     1021 aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac
     1081 aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg
     1141 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     1201 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     1261 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     1321 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     1381 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     1441 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     1501 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     1561 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     1621 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     1681 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     1741 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     1801 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     1861 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga
     1921 tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg
     1981 cgcggcttaa ctcaagcgtt aga
//
LOCUS       DAJTUP010000440         2000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.guided.241, whole genome shotgun sequence.
ACCESSION   DAJTUP010000440 DAJTUP010000000
VERSION     DAJTUP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.guided.241"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            2..475
                     /locus_tag="OO468_004399"
     CDS             2..475
                     /locus_tag="OO468_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR6261242.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            570..1094
                     /gene="sat2"
                     /locus_tag="OO468_004400"
     CDS             570..1094
                     /gene="sat2"
                     /locus_tag="OO468_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR6261243.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1152..1940
                     /gene="aadA1"
                     /locus_tag="OO468_004401"
     CDS             1152..1940
                     /gene="aadA1"
                     /locus_tag="OO468_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR6261244.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          542 a          474 c          503 g          481 t
ORIGIN      
        1 tgtgaaacta tcactaatgg tagctatatc gaagaatgga gttatcggga atggccctga
       61 tattccatgg agtgccaaag gtgaacagct cctgtttaaa gctattacct ataaccaatg
      121 gctgttggtt ggacgcaaga cttttgaatc aatgggagca ttacccaacc gaaagtatgc
      181 ggtcgtaaca cgttcaagtt ttacatctga caatgagaac gtattgatct ttccatcaat
      241 taaagatgct ttaaccaacc taaagaaaat aacggatcat gtcattgttt caggtggtgg
      301 ggagatatac aaaagcctga tcgatcaagt agatacacta catatatcta caatagacat
      361 cgagccggaa ggtgatgttt actttcctga aatccccagc aattttaggc cagtttttac
      421 ccaagacttc gcctctaaca taaattatag ttaccaaatc tggcaaaagg gttaacaagt
      481 ggcagcaacg gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg ccaggtttgc
      541 gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg
      601 cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat
      661 ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct
      721 cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg
      781 tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg
      841 tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt
      901 gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg
      961 cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata
     1021 aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac
     1081 aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg
     1141 agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     1201 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     1261 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     1321 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     1381 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     1441 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     1501 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     1561 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     1621 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     1681 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     1741 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     1801 cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga caagaagatc
     1861 gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa ggcgagatca
     1921 ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc
     1981 ggcttaactc aagcgttaga
//
LOCUS       DAJTUP010000441         1703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.guided.253, whole genome shotgun sequence.
ACCESSION   DAJTUP010000441 DAJTUP010000000
VERSION     DAJTUP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.guided.253"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            41..1173
                     /locus_tag="OO468_004402"
                     /pseudo
     CDS             41..1173
                     /locus_tag="OO468_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
     gene            1262..>1703
                     /gene="blc"
                     /locus_tag="OO468_004403"
     CDS             1262..>1703
                     /gene="blc"
                     /locus_tag="OO468_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR6261245.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTI"
BASE COUNT          426 a          445 c          423 g          409 t
ORIGIN      
        1 cccgcctatg gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg
       61 cgccttatta attaccgcct cttactccac atttgctgcc cctcaacaaa tcaacgatat
      121 tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc
      181 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
      241 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
      301 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa
      361 atactggcct gaacttaccg ctaaacagtg gaatgggatc acactattac atcttgcgac
      421 ctacaccgct ggcggcctgc cattgcaggt gccggatgaa gtgaaatcct caagcgactt
      481 gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc
      541 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
      601 gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt
      661 acctccccca gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt
      721 ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc
      781 ccgctgggtg caaagcaatt tacccccctt gatatcaatg agaaaacgct tcaacaaggg
      841 atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg cctgggctgg
      901 gaaatgctgg actggccggt caatcctgac agcatcatta acggcagtga caataaaatt
      961 gcactggcag cacgcccggt aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca
     1021 tgggtacata aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa
     1081 aaagagctgg gtatcgtgat gctggctaac aaaaactatc ccaatccagc gggagtcgcc
     1141 gccgcctgtc agattctcaa cgctctacag taaaatttca tcgggtccga attttcggac
     1201 cttttctgcg cttttccttg ctgtcatcta cacttagaaa aaaaccagta aggaaacatt
     1261 tatgcgcctg ctccctctcg ttgccgcagc gacagctgca tttctggtcg ttgcctgtag
     1321 ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat ttcgacgcca aacgttatct
     1381 cggtacctgg tatgagattg cccgttttga tcaccgtttt gaacgtggac tggaaaaagt
     1441 caccgcaaca tacagcctgc gtgatgacgg cggcctgaat gtcattaata aaggctataa
     1501 ccctgacaga ggaatgtggc agcagagtga agggaaagcg tactttaccg gcgcaccaac
     1561 tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat ggcggttata acgttattgc
     1621 actcgatcgg gaataccgcc atgcgctggt ttgcggcccg gaccgcgact acctgtggat
     1681 actctcccgc acgccaacca ttt
//
LOCUS       DAJTUP010000442         1173 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600818
            SAMEA8900438-rid15817323.guided.287, whole genome shotgun sequence.
ACCESSION   DAJTUP010000442 DAJTUP010000000
VERSION     DAJTUP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900438
            Sequence Read Archive: ERR6004817
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477731.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477731.1
            Genome Coverage       :: 24x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 21:56:13
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,404
            CDSs (total)                      :: 4,339
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 65
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 54
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 363
            CDSs (without protein)            :: 363
            Pseudo Genes (ambiguous residues) :: 0 of 363
            Pseudo Genes (frameshifted)       :: 176 of 363
            Pseudo Genes (incomplete)         :: 84 of 363
            Pseudo Genes (internal stop)      :: 140 of 363
            Pseudo Genes (multiple problems)  :: 36 of 363
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900438-rid15817323.guided.287"
                     /serovar="S. flexneri 2b"
                     /isolate="600818"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-07-24"
     gene            41..1173
                     /locus_tag="OO468_004404"
                     /pseudo
     CDS             41..1173
                     /locus_tag="OO468_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
BASE COUNT          310 a          304 c          292 g          267 t
ORIGIN      
        1 cccgcctatg gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg
       61 cgccttatta attaccgcct cttactccac atttgctgcc cctcaacaaa tcaacgatat
      121 tgtgcatcgc acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc
      181 ggtaatttat cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa
      241 gcagcccgtc acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg
      301 cgtgcttggt ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa
      361 atactggcct gaacttaccg ctaaacagtg gaatgggatc acactattac atcttgcgac
      421 ctacaccgct ggcggcctgc cattgcaggt gccggatgaa gtgaaatcct caagcgactt
      481 gctgcgcttc tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc
      541 caactccagt atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga
      601 gcaggcgatg caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt
      661 acctccccca gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt
      721 ttcgccaggg gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc
      781 ccgctgggtg caaagcaatt tacccccctt gatatcaatg agaaaacgct tcaacaaggg
      841 atacaactgg cacaatctcg ctactggcaa accggcgata tgtatcaggg cctgggctgg
      901 gaaatgctgg actggccggt caatcctgac agcatcatta acggcagtga caataaaatt
      961 gcactggcag cacgcccggt aaaagcgatt aagcccccaa ctcctgcagt acgcgcatca
     1021 tgggtacata aaacgggggc gaccggcgga tttggtagct atgtcgcgtt tattccagaa
     1081 aaagagctgg gtatcgtgat gctggctaac aaaaactatc ccaatccagc gagagtcgcc
     1141 gccgcctgtc agattctcaa cgctctacag taa
//
